USP53 cDNA ORF clone, Otolemur garnettii(small-eared galago)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP53
Entrez Gene ID	100954665
Full Name	ubiquitin specific peptidase 53
Gene Type	protein-coding
Organism	Otolemur garnettii(small-eared galago)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003800074.1	XP_003800122.1	inactive ubiquitin carboxyl-terminal hydrolase 53
XM_023519242.1	XP_023375010.1	inactive ubiquitin carboxyl-terminal hydrolase 53

Pathways from BioSystems
Gene Ontology

The following USP53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOt33438	XM_023519242.1 Latest version!	Otolemur garnettii ubiquitin specific peptidase 53 (USP53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OOt21231	XM_003800074.1 Latest version!	Otolemur garnettii ubiquitin specific peptidase 53 (USP53), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP53 ( XM_023519242.1 ) cDNA ORF clone, Otolemur garnettii(small-eared galago) -> XP_023375010.1 Otolemur garnettii ubiquitin specific peptidase 53 (USP53), mRNA.

Price & Availability↑

CloneID	OOt33438
Clone ID Related Accession (Same CDS sequence)	XM_023519242.1
Accession Version	XM_023519242.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1515600000000
Organism	Otolemur garnettii(small-eared galago)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003852559.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Otolemur garnettii Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACG CTGCAGAGTG CTTCGAAAAT ATCCTGGAGC GGATTCATTT TCACATAGTG 
CCAAGCAGAG ATGCAGACAT GTGTACCTCC AGGTCTTGTA TCACTCACCA GAAGTTTGCG 
ATGACTCTTT ATGAACAGTG CGTGTGTCGC AGCTGCGGAG CATCGTCAGA CCCTCTGCCT 
TTTACAGAAT TTGTGCGGTA CATTTCGACC ACAGCCCTAT GCAATGAAGT TGAAAGAATG 
GTGGAAAGGC ATGAACGCTT TAAACCTGAA ATGTTTGCCG AATTGCTACA AGCAGCAAAT 
ACAACAGATG ACTATAGGAA ATGTCCTAGT AACTGTGGCC AAAAAATAAA AATTCGCCGT 
GTTTTAATGA ATTGCCCAGA GATTGTCACA GTTGGTTTAG TCTGGGACTC GGAGCATTCC 
GACTTGACTG AAGACGTTGT TCGAAATCTA GCAACACAAC TTTATCTTCC TGGGCTTTTT 
TATAGAGTTA CTGATGAAAA TGCCAAAAAT AGTGAACTTC ACCTTGTTGG TGTGATCTGC 
TATACCAGCC GTCATTATTG TGCCTTTGCT TTTCATACGA AAAGTTCCAA ATGGGTATTT 
TTCGATGATG CAAATGTGAA AGAGATTGGA ACTAGATGGA AAGATGTTGT CTCCAAGTGT 
ATCCGATGCC ACTTTCAGCC ACTGCTTTTG TTTTACGCAA ACCCAGATGG CACAGCAGTT 
TCTACTGAAG ACGCACTCAG GCAGGTTGTC AACTGGTCAC ATTACAAGTC TGTTGCAGAA 
AATATGGGAT GTGAAAAGCC CACAGTTTCA AAGTTAGATA ATTCAAAAGA AAATGGATTT 
GGTGACCAGG CAAAACAGAG AGAAAATCAG AAATTTCAAA CCAGTACTAT TTCATCATTT 
AACCGGAGCC ACGCTCAGAC GAGTGGAGGC AGAGGACCAG TTAAATTAAG TCATATTGAT 
CAAAGGGAAA AGATAAAAGA CATTTCCAGA GAATGTGCTC TAAAAGCTAT TGAACAGAAA 
AACTTACTTT CTTCACAAAG GAAAGATTTA GAGAAGGGAC AAAGAAAAGA TTTAGGCCGA 
CATAGAGAAT TGGTTGAAGA AGATCTTTCA CATTTCCAGT CTGGATCACC TCCTGCCACA 
AACGGCTTTA GACAACATGG GAATCCACAT CTGTATCATA GTCAAGGAAA AGGACCATGT 
AAACATGACC GAGTGACCTG CCAGAGTCGA GCTTCTGCAC AAGTAAGTTC AAGTAAATCC 
CAGGTTCTTG TTCCAGGAGA GAAAATAACC GGCAAAGTGA GGAGTGACAG TGGCCCTGGA 
TATGATACAG ATGGCAGCCA AGATTCTAGG GATAAAGGAA GCAGCTGTCA TAGCAGCAGT 
AAAAGTCGGA ACCGAGGTTG GAAACCTATG AGAGAAACAT TAAATGTTGA CAGTATTTTT 
AATGAAAGTG AAAAAAGACA GCATAGTCCA AGACATAAAT CAAGCATTGG TAACAAACCT 
AAAGGGAGCA AAGATCAGAG TTTCAATAAT TGGCCAAAAG AGAATCCAAA GCAAAAAGGC 
TTAATGACCA TCTATGAAGA TGAAACAAAG CAGGAAGTAG GAAGCAGAAG TTCCCTGGAA 
TCTAATGGAA AAGGGACAGA GAAAAATAAA GGCCTCGCGG ACAGCAAAGT TTATGGTGAT 
AACTGGCAGA TGCAAAGGAC GGAGTCTGGA TACGAAAGCA GCGATCACAT CAGTAATGGA 
TCTGCTAATT TGGACTCCCC TGTTATCGAC GGAAATGGCA CAGCAGTGGA GATCAGTGGT 
GTTAAAGAAA CAGTATTCTT CAGTGACCAG ATTAAGACAA ACAGCCTGAA ACATGAAGGT 
GTGGACTGTA ACTCTCTTCA GAGCAAAAAC CACTTAGAAG GCTTTAGAAA AGAACTTAAG 
AATTGGGAAG CAGGCTATAA ATCTCATGAG TTCCACCCAG AATCACATCT CCAAATAAAA 
AATAGTTTGA TAAAAAGGTC ACATGTTCAT GAAACCAATG GAAAGTTACT TCCTCCACCC 
AGTCCACAAA CACTCAAGGA CCATAGTGCA AGAGAAACTA TCCATCAGGC AGATGAACAG 
AAGGCTGATA AAACAGATGA ATGCAGATTT TCTGAGAGGC TTAATATAGA AAATTCTGAG 
AGAAGAGGTT TGCCTTTTCA TGGTGATAAC TCTGCTTCTG GGAAGAGAGT GAACAGCAGT 
GAAATAGTCT TACCATCTTC ATTGCGGTCT TCACACATGA GAACTGTTGG GTTAAAGCCA 
GAAATTGCCC CCCTCTTCCA GCAACAAAAT ATGATGGAAC AATGTTACTC TGAGAACTCT 
CAGTCCAAGG AACATACAGT TCAGCCAACC TGCAGATCTG ATGGGTGTCA GATGCCAAAA 
CCTTTTTGCC AGAATCTACC ACCCCCGTTG CCACCAAAGA AATATGCTGT AACTAGTGTG 
CTACAGTCAG AGAAAAATGA ATCTACAGCT GATGTCAATC TTACAGAGGT GTTTCAAGCT 
AATTCTTCTA GCCTTCCGAA GCACAGTTTA AGTATAGCTT CAGAACCAAA TTTAGAAATG 
AGTACATATG TGAATGATGA AAGGCGGAAA GGAACATTTC AAGTGAAAGA ATGTGTTGAT 
AACACATCAG ACTACCCATC CAGCTCTTCA GCTAATGATT TTCAGGCAAA CTCAGGTGCA 
ATTATTGATG CATGTTGCCA ACCAGAACTA GACTCTAGTT CTCCCAGTAC AAATGAGACA 
ATATCATTAA CCACCTATTT TTCAGTTGAT AGCTGCATGA CTGACACATA CAGATTGAAG 
TATCATCAGA GACCTAAGCT CTGTTTTCCA GAAAGCAGCG GCTTTTGTAA TGATAATTCA 
GTATCTCAGA GTGACAGAGA GGTAGGGCCT GTGTTACATA ATAGTCTTGG TTCAGGCTAT 
CCTTCTGAGC AAAGATGTAA ACCTAGCATA CACTGTAATA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023375010.1
CDS	200..3184
Translation	MDDAAECFENILERIHFHIVPSRDADMCTSRSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMVERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTVGLVWDSEHSDLTEDVVRNLATQLYLPGLFYRVTDENAKNSELHLVGVICYTSRHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVVNWSHYKSVAENMGCEKPTVSKLDNSKENGFGDQAKQRENQKFQTSTISSFNRSHAQTSGGRGPVKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRELVEEDLSHFQSGSPPATNGFRQHGNPHLYHSQGKGPCKHDRVTCQSRASAQVSSSKSQVLVPGEKITGKVRSDSGPGYDTDGSQDSRDKGSSCHSSSKSRNRGWKPMRETLNVDSIFNESEKRQHSPRHKSSIGNKPKGSKDQSFNNWPKENPKQKGLMTIYEDETKQEVGSRSSLESNGKGTEKNKGLADSKVYGDNWQMQRTESGYESSDHISNGSANLDSPVIDGNGTAVEISGVKETVFFSDQIKTNSLKHEGVDCNSLQSKNHLEGFRKELKNWEAGYKSHEFHPESHLQIKNSLIKRSHVHETNGKLLPPPSPQTLKDHSARETIHQADEQKADKTDECRFSERLNIENSERRGLPFHGDNSASGKRVNSSEIVLPSSLRSSHMRTVGLKPEIAPLFQQQNMMEQCYSENSQSKEHTVQPTCRSDGCQMPKPFCQNLPPPLPPKKYAVTSVLQSEKNESTADVNLTEVFQANSSSLPKHSLSIASEPNLEMSTYVNDERRKGTFQVKECVDNTSDYPSSSSANDFQANSGAIIDACCQPELDSSSPSTNETISLTTYFSVDSCMTDTYRLKYHQRPKLCFPESSGFCNDNSVSQSDREVGPVLHNSLGSGYPSEQRCKPSIHCNR

Target ORF information:

RefSeq Version	XM_023519242.1
Organism	Otolemur garnettii(small-eared galago)
Definition	Otolemur garnettii ubiquitin specific peptidase 53 (USP53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023519242.1

ORF Insert Sequence:

ATGGATGACG CTGCAGAGTG CTTCGAAAAT ATCCTGGAGC GGATTCATTT TCACATAGTG 
CCAAGCAGAG ATGCAGACAT GTGTACCTCC AGGTCTTGTA TCACTCACCA GAAGTTTGCG 
ATGACTCTTT ATGAACAGTG CGTGTGTCGC AGCTGCGGAG CATCGTCAGA CCCTCTGCCT 
TTTACAGAAT TTGTGCGGTA CATTTCGACC ACAGCCCTAT GCAATGAAGT TGAAAGAATG 
GTGGAAAGGC ATGAACGCTT TAAACCTGAA ATGTTTGCCG AATTGCTACA AGCAGCAAAT 
ACAACAGATG ACTATAGGAA ATGTCCTAGT AACTGTGGCC AAAAAATAAA AATTCGCCGT 
GTTTTAATGA ATTGCCCAGA GATTGTCACA GTTGGTTTAG TCTGGGACTC GGAGCATTCC 
GACTTGACTG AAGACGTTGT TCGAAATCTA GCAACACAAC TTTATCTTCC TGGGCTTTTT 
TATAGAGTTA CTGATGAAAA TGCCAAAAAT AGTGAACTTC ACCTTGTTGG TGTGATCTGC 
TATACCAGCC GTCATTATTG TGCCTTTGCT TTTCATACGA AAAGTTCCAA ATGGGTATTT 
TTCGATGATG CAAATGTGAA AGAGATTGGA ACTAGATGGA AAGATGTTGT CTCCAAGTGT 
ATCCGATGCC ACTTTCAGCC ACTGCTTTTG TTTTACGCAA ACCCAGATGG CACAGCAGTT 
TCTACTGAAG ACGCACTCAG GCAGGTTGTC AACTGGTCAC ATTACAAGTC TGTTGCAGAA 
AATATGGGAT GTGAAAAGCC CACAGTTTCA AAGTTAGATA ATTCAAAAGA AAATGGATTT 
GGTGACCAGG CAAAACAGAG AGAAAATCAG AAATTTCAAA CCAGTACTAT TTCATCATTT 
AACCGGAGCC ACGCTCAGAC GAGTGGAGGC AGAGGACCAG TTAAATTAAG TCATATTGAT 
CAAAGGGAAA AGATAAAAGA CATTTCCAGA GAATGTGCTC TAAAAGCTAT TGAACAGAAA 
AACTTACTTT CTTCACAAAG GAAAGATTTA GAGAAGGGAC AAAGAAAAGA TTTAGGCCGA 
CATAGAGAAT TGGTTGAAGA AGATCTTTCA CATTTCCAGT CTGGATCACC TCCTGCCACA 
AACGGCTTTA GACAACATGG GAATCCACAT CTGTATCATA GTCAAGGAAA AGGACCATGT 
AAACATGACC GAGTGACCTG CCAGAGTCGA GCTTCTGCAC AAGTAAGTTC AAGTAAATCC 
CAGGTTCTTG TTCCAGGAGA GAAAATAACC GGCAAAGTGA GGAGTGACAG TGGCCCTGGA 
TATGATACAG ATGGCAGCCA AGATTCTAGG GATAAAGGAA GCAGCTGTCA TAGCAGCAGT 
AAAAGTCGGA ACCGAGGTTG GAAACCTATG AGAGAAACAT TAAATGTTGA CAGTATTTTT 
AATGAAAGTG AAAAAAGACA GCATAGTCCA AGACATAAAT CAAGCATTGG TAACAAACCT 
AAAGGGAGCA AAGATCAGAG TTTCAATAAT TGGCCAAAAG AGAATCCAAA GCAAAAAGGC 
TTAATGACCA TCTATGAAGA TGAAACAAAG CAGGAAGTAG GAAGCAGAAG TTCCCTGGAA 
TCTAATGGAA AAGGGACAGA GAAAAATAAA GGCCTCGCGG ACAGCAAAGT TTATGGTGAT 
AACTGGCAGA TGCAAAGGAC GGAGTCTGGA TACGAAAGCA GCGATCACAT CAGTAATGGA 
TCTGCTAATT TGGACTCCCC TGTTATCGAC GGAAATGGCA CAGCAGTGGA GATCAGTGGT 
GTTAAAGAAA CAGTATTCTT CAGTGACCAG ATTAAGACAA ACAGCCTGAA ACATGAAGGT 
GTGGACTGTA ACTCTCTTCA GAGCAAAAAC CACTTAGAAG GCTTTAGAAA AGAACTTAAG 
AATTGGGAAG CAGGCTATAA ATCTCATGAG TTCCACCCAG AATCACATCT CCAAATAAAA 
AATAGTTTGA TAAAAAGGTC ACATGTTCAT GAAACCAATG GAAAGTTACT TCCTCCACCC 
AGTCCACAAA CACTCAAGGA CCATAGTGCA AGAGAAACTA TCCATCAGGC AGATGAACAG 
AAGGCTGATA AAACAGATGA ATGCAGATTT TCTGAGAGGC TTAATATAGA AAATTCTGAG 
AGAAGAGGTT TGCCTTTTCA TGGTGATAAC TCTGCTTCTG GGAAGAGAGT GAACAGCAGT 
GAAATAGTCT TACCATCTTC ATTGCGGTCT TCACACATGA GAACTGTTGG GTTAAAGCCA 
GAAATTGCCC CCCTCTTCCA GCAACAAAAT ATGATGGAAC AATGTTACTC TGAGAACTCT 
CAGTCCAAGG AACATACAGT TCAGCCAACC TGCAGATCTG ATGGGTGTCA GATGCCAAAA 
CCTTTTTGCC AGAATCTACC ACCCCCGTTG CCACCAAAGA AATATGCTGT AACTAGTGTG 
CTACAGTCAG AGAAAAATGA ATCTACAGCT GATGTCAATC TTACAGAGGT GTTTCAAGCT 
AATTCTTCTA GCCTTCCGAA GCACAGTTTA AGTATAGCTT CAGAACCAAA TTTAGAAATG 
AGTACATATG TGAATGATGA AAGGCGGAAA GGAACATTTC AAGTGAAAGA ATGTGTTGAT 
AACACATCAG ACTACCCATC CAGCTCTTCA GCTAATGATT TTCAGGCAAA CTCAGGTGCA 
ATTATTGATG CATGTTGCCA ACCAGAACTA GACTCTAGTT CTCCCAGTAC AAATGAGACA 
ATATCATTAA CCACCTATTT TTCAGTTGAT AGCTGCATGA CTGACACATA CAGATTGAAG 
TATCATCAGA GACCTAAGCT CTGTTTTCCA GAAAGCAGCG GCTTTTGTAA TGATAATTCA 
GTATCTCAGA GTGACAGAGA GGTAGGGCCT GTGTTACATA ATAGTCTTGG TTCAGGCTAT 
CCTTCTGAGC AAAGATGTAA ACCTAGCATA CACTGTAATA GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP53 ( XM_003800074.1 ) cDNA ORF clone, Otolemur garnettii(small-eared galago) -> XP_003800122.1 Otolemur garnettii ubiquitin specific peptidase 53 (USP53), mRNA.

Price & Availability↑

CloneID	OOt21231
Clone ID Related Accession (Same CDS sequence)	XM_003800074.1
Accession Version	XM_003800074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3246bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433952000000
Organism	Otolemur garnettii(small-eared galago)
Product	inactive ubiquitin carboxyl-terminal hydrolase 53
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003852559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Otolemur garnettii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGACTAAAA GACTAGGGAA ACTTGAAGAT CTTATTTATG GAGCAGGTGA TAAATGGGTT 
TTGTGGCAAC TGGATATATT CCGACGAAGC TTGCGGGTTC TCACTGGACA TATTTGTCAG 
GGAGACGCCT GCATATTTTG TGCATTGAAG ACAATATTTG CCCAGTTTCA ACACAGTCGG 
GAAAAAGCAC TTCCCTCGGA TAACATAAGG CATGCTCTTG CAGAAAGCTT CAAAGACGAG 
CAGCGATTTC AACTTGGCCT TATGGATGAC GCTGCAGAGT GCTTCGAAAA TATCCTGGAG 
CGGATTCATT TTCACATAGT GCCAAGCAGA GATGCAGACA TGTGTACCTC CAGGTCTTGT 
ATCACTCACC AGAAGTTTGC GATGACTCTT TATGAACAGT GCGTGTGTCG CAGCTGCGGA 
GCATCGTCAG ACCCTCTGCC TTTTACAGAA TTTGTGCGGT ACATTTCGAC CACAGCCCTA 
TGCAATGAAG TTGAAAGAAT GGTGGAAAGG CATGAACGCT TTAAACCTGA AATGTTTGCC 
GAATTGCTAC AAGCAGCAAA TACAACAGAT GACTATAGGA AATGTCCTAG TAACTGTGGC 
CAAAAAATAA AAATTCGCCG TGTTTTAATG AATTGCCCAG AGATTGTCAC AGTTGGTTTA 
GTCTGGGACT CGGAGCATTC CGACTTGACT GAAGACGTTG TTCGAAATCT AGCAACACAA 
CTTTATCTTC CTGGGCTTTT TTATAGAGTT ACTGATGAAA ATGCCAAAAA TAGTGAACTT 
CACCTTGTTG GTGTGATCTG CTATACCAGC CGTCATTATT GTGCCTTTGC TTTTCATACG 
AAAAGTTCCA AATGGGTATT TTTCGATGAT GCAAATGTGA AAGAGATTGG AACTAGATGG 
AAAGATGTTG TCTCCAAGTG TATCCGATGC CACTTTCAGC CACTGCTTTT GTTTTACGCA 
AACCCAGATG GCACAGCAGT TTCTACTGAA GACGCACTCA GGCAGGTTGT CAACTGGTCA 
CATTACAAGT CTGTTGCAGA AAATATGGGA TGTGAAAAGC CCACAGTTTC AAAGTTAGAT 
AATTCAAAAG AAAATGGATT TGGTGACCAG GCAAAACAGA GAGAAAATCA GAAATTTCAA 
ACCAGTACTA TTTCATCATT TAACCGGAGC CACGCTCAGA CGAGTGGAGG CAGAGGACCA 
GTTAAATTAA GTCATATTGA TCAAAGGGAA AAGATAAAAG ACATTTCCAG AGAATGTGCT 
CTAAAAGCTA TTGAACAGAA AAACTTACTT TCTTCACAAA GGAAAGATTT AGAGAAGGGA 
CAAAGAAAAG ATTTAGGCCG ACATAGAGAA TTGGTTGAAG AAGATCTTTC ACATTTCCAG 
TCTGGATCAC CTCCTGCCAC AAACGGCTTT AGACAACATG GGAATCCACA TCTGTATCAT 
AGTCAAGGAA AAGGACCATG TAAACATGAC CGAGTGACCT GCCAGAGTCG AGCTTCTGCA 
CAAGTAAGTT CAAGTAAATC CCAGGTTCTT GTTCCAGGAG AGAAAATAAC CGGCAAAGTG 
AGGAGTGACA GTGGCCCTGG ATATGATACA GATGGCAGCC AAGATTCTAG GGATAAAGGA 
AGCAGCTGTC ATAGCAGCAG TAAAAGTCGG AACCGAGGTT GGAAACCTAT GAGAGAAACA 
TTAAATGTTG ACAGTATTTT TAATGAAAGT GAAAAAAGAC AGCATAGTCC AAGACATAAA 
TCAAGCATTG GTAACAAACC TAAAGGGAGC AAAGATCAGA GTTTCAATAA TTGGCCAAAA 
GAGAATCCAA AGCAAAAAGG CTTAATGACC ATCTATGAAG ATGAAACAAA GCAGGAAGTA 
GGAAGCAGAA GTTCCCTGGA ATCTAATGGA AAAGGGACAG AGAAAAATAA AGGCCTCGCG 
GACAGCAAAG TTTATGGTGA TAACTGGCAG ATGCAAAGGA CGGAGTCTGG ATACGAAAGC 
AGCGATCACA TCAGTAATGG ATCTGCTAAT TTGGACTCCC CTGTTATCGA CGGAAATGGC 
ACAGCAGTGG AGATCAGTGG TGTTAAAGAA ACAGTATTCT TCAGTGACCA GATTAAGACA 
AACAGCCTGA AACATGAAGG TGTGGACTGT AACTCTCTTC AGAGCAAAAA CCACTTAGAA 
GGCTTTAGAA AAGAACTTAA GAATTGGGAA GCAGGCTATA AATCTCATGA GTTCCACCCA 
GAATCACATC TCCAAATAAA AAATAGTTTG ATAAAAAGGT CACATGTTCA TGAAACCAAT 
GGAAAGTTAC TTCCTCCACC CAGTCCACAA ACACTCAAGG ACCATAGTGC AAGAGAAACT 
ATCCATCAGG CAGATGAACA GAAGGCTGAT AAAACAGATG AATGCAGATT TTCTGAGAGG 
CTTAATATAG AAAATTCTGA GAGAAGAGGT TTGCCTTTTC ATGGTGATAA CTCTGCTTCT 
GGGAAGAGAG TGAACAGCAG TGAAATAGTC TTACCATCTT CATTGCGGTC TTCACACATG 
AGAACTGTTG GGTTAAAGCC AGAAATTGCC CCCCTCTTCC AGCAACAAAA TATGATGGAA 
CAATGTTACT CTGAGAACTC TCAGTCCAAG GAACATACAG TTCAGCCAAC CTGCAGATCT 
GATGGGTGTC AGATGCCAAA ACCTTTTTGC CAGAATCTAC CACCCCCGTT GCCACCAAAG 
AAATATGCTG TAACTAGTGT GCTACAGTCA GAGAAAAATG AATCTACAGC TGATGTCAAT 
CTTACAGAGG TGTTTCAAGC TAATTCTTCT AGCCTTCCGA AGCACAGTTT AAGTATAGCT 
TCAGAACCAA ATTTAGAAAT GAGTACATAT GTGAATGATG AAAGGCGGAA AGGAACATTT 
CAAGTGAAAG AATGTGTTGA TAACACATCA GACTACCCAT CCAGCTCTTC AGCTAATGAT 
TTTCAGGCAA ACTCAGGTGC AATTATTGAT GCATGTTGCC AACCAGAACT AGACTCTAGT 
TCTCCCAGTA CAAATGAGAC AATATCATTA ACCACCTATT TTTCAGTTGA TAGCTGCATG 
ACTGACACAT ACAGATTGAA GTATCATCAG AGACCTAAGC TCTGTTTTCC AGAAAGCAGC 
GGCTTTTGTA ATGATAATTC AGTATCTCAG AGTGACAGAG AGGTAGGGCC TGTGTTACAT 
AATAGTCTTG GTTCAGGCTA TCCTTCTGAG CAAAGATGTA AACCTAGCAT ACACTGTAAT 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003800122.1
CDS	1..3246
Translation	MTKRLGKLEDLIYGAGDKWVLWQLDIFRRSLRVLTGHICQGDACIFCALKTIFAQFQHSREKALPSDNIRHALAESFKDEQRFQLGLMDDAAECFENILERIHFHIVPSRDADMCTSRSCITHQKFAMTLYEQCVCRSCGASSDPLPFTEFVRYISTTALCNEVERMVERHERFKPEMFAELLQAANTTDDYRKCPSNCGQKIKIRRVLMNCPEIVTVGLVWDSEHSDLTEDVVRNLATQLYLPGLFYRVTDENAKNSELHLVGVICYTSRHYCAFAFHTKSSKWVFFDDANVKEIGTRWKDVVSKCIRCHFQPLLLFYANPDGTAVSTEDALRQVVNWSHYKSVAENMGCEKPTVSKLDNSKENGFGDQAKQRENQKFQTSTISSFNRSHAQTSGGRGPVKLSHIDQREKIKDISRECALKAIEQKNLLSSQRKDLEKGQRKDLGRHRELVEEDLSHFQSGSPPATNGFRQHGNPHLYHSQGKGPCKHDRVTCQSRASAQVSSSKSQVLVPGEKITGKVRSDSGPGYDTDGSQDSRDKGSSCHSSSKSRNRGWKPMRETLNVDSIFNESEKRQHSPRHKSSIGNKPKGSKDQSFNNWPKENPKQKGLMTIYEDETKQEVGSRSSLESNGKGTEKNKGLADSKVYGDNWQMQRTESGYESSDHISNGSANLDSPVIDGNGTAVEISGVKETVFFSDQIKTNSLKHEGVDCNSLQSKNHLEGFRKELKNWEAGYKSHEFHPESHLQIKNSLIKRSHVHETNGKLLPPPSPQTLKDHSARETIHQADEQKADKTDECRFSERLNIENSERRGLPFHGDNSASGKRVNSSEIVLPSSLRSSHMRTVGLKPEIAPLFQQQNMMEQCYSENSQSKEHTVQPTCRSDGCQMPKPFCQNLPPPLPPKKYAVTSVLQSEKNESTADVNLTEVFQANSSSLPKHSLSIASEPNLEMSTYVNDERRKGTFQVKECVDNTSDYPSSSSANDFQANSGAIIDACCQPELDSSSPSTNETISLTTYFSVDSCMTDTYRLKYHQRPKLCFPESSGFCNDNSVSQSDREVGPVLHNSLGSGYPSEQRCKPSIHCNR

Target ORF information:

RefSeq Version	XM_003800074.1
Organism	Otolemur garnettii(small-eared galago)
Definition	Otolemur garnettii ubiquitin specific peptidase 53 (USP53), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003800074.1

ORF Insert Sequence:

ATGACTAAAA GACTAGGGAA ACTTGAAGAT CTTATTTATG GAGCAGGTGA TAAATGGGTT 
TTGTGGCAAC TGGATATATT CCGACGAAGC TTGCGGGTTC TCACTGGACA TATTTGTCAG 
GGAGACGCCT GCATATTTTG TGCATTGAAG ACAATATTTG CCCAGTTTCA ACACAGTCGG 
GAAAAAGCAC TTCCCTCGGA TAACATAAGG CATGCTCTTG CAGAAAGCTT CAAAGACGAG 
CAGCGATTTC AACTTGGCCT TATGGATGAC GCTGCAGAGT GCTTCGAAAA TATCCTGGAG 
CGGATTCATT TTCACATAGT GCCAAGCAGA GATGCAGACA TGTGTACCTC CAGGTCTTGT 
ATCACTCACC AGAAGTTTGC GATGACTCTT TATGAACAGT GCGTGTGTCG CAGCTGCGGA 
GCATCGTCAG ACCCTCTGCC TTTTACAGAA TTTGTGCGGT ACATTTCGAC CACAGCCCTA 
TGCAATGAAG TTGAAAGAAT GGTGGAAAGG CATGAACGCT TTAAACCTGA AATGTTTGCC 
GAATTGCTAC AAGCAGCAAA TACAACAGAT GACTATAGGA AATGTCCTAG TAACTGTGGC 
CAAAAAATAA AAATTCGCCG TGTTTTAATG AATTGCCCAG AGATTGTCAC AGTTGGTTTA 
GTCTGGGACT CGGAGCATTC CGACTTGACT GAAGACGTTG TTCGAAATCT AGCAACACAA 
CTTTATCTTC CTGGGCTTTT TTATAGAGTT ACTGATGAAA ATGCCAAAAA TAGTGAACTT 
CACCTTGTTG GTGTGATCTG CTATACCAGC CGTCATTATT GTGCCTTTGC TTTTCATACG 
AAAAGTTCCA AATGGGTATT TTTCGATGAT GCAAATGTGA AAGAGATTGG AACTAGATGG 
AAAGATGTTG TCTCCAAGTG TATCCGATGC CACTTTCAGC CACTGCTTTT GTTTTACGCA 
AACCCAGATG GCACAGCAGT TTCTACTGAA GACGCACTCA GGCAGGTTGT CAACTGGTCA 
CATTACAAGT CTGTTGCAGA AAATATGGGA TGTGAAAAGC CCACAGTTTC AAAGTTAGAT 
AATTCAAAAG AAAATGGATT TGGTGACCAG GCAAAACAGA GAGAAAATCA GAAATTTCAA 
ACCAGTACTA TTTCATCATT TAACCGGAGC CACGCTCAGA CGAGTGGAGG CAGAGGACCA 
GTTAAATTAA GTCATATTGA TCAAAGGGAA AAGATAAAAG ACATTTCCAG AGAATGTGCT 
CTAAAAGCTA TTGAACAGAA AAACTTACTT TCTTCACAAA GGAAAGATTT AGAGAAGGGA 
CAAAGAAAAG ATTTAGGCCG ACATAGAGAA TTGGTTGAAG AAGATCTTTC ACATTTCCAG 
TCTGGATCAC CTCCTGCCAC AAACGGCTTT AGACAACATG GGAATCCACA TCTGTATCAT 
AGTCAAGGAA AAGGACCATG TAAACATGAC CGAGTGACCT GCCAGAGTCG AGCTTCTGCA 
CAAGTAAGTT CAAGTAAATC CCAGGTTCTT GTTCCAGGAG AGAAAATAAC CGGCAAAGTG 
AGGAGTGACA GTGGCCCTGG ATATGATACA GATGGCAGCC AAGATTCTAG GGATAAAGGA 
AGCAGCTGTC ATAGCAGCAG TAAAAGTCGG AACCGAGGTT GGAAACCTAT GAGAGAAACA 
TTAAATGTTG ACAGTATTTT TAATGAAAGT GAAAAAAGAC AGCATAGTCC AAGACATAAA 
TCAAGCATTG GTAACAAACC TAAAGGGAGC AAAGATCAGA GTTTCAATAA TTGGCCAAAA 
GAGAATCCAA AGCAAAAAGG CTTAATGACC ATCTATGAAG ATGAAACAAA GCAGGAAGTA 
GGAAGCAGAA GTTCCCTGGA ATCTAATGGA AAAGGGACAG AGAAAAATAA AGGCCTCGCG 
GACAGCAAAG TTTATGGTGA TAACTGGCAG ATGCAAAGGA CGGAGTCTGG ATACGAAAGC 
AGCGATCACA TCAGTAATGG ATCTGCTAAT TTGGACTCCC CTGTTATCGA CGGAAATGGC 
ACAGCAGTGG AGATCAGTGG TGTTAAAGAA ACAGTATTCT TCAGTGACCA GATTAAGACA 
AACAGCCTGA AACATGAAGG TGTGGACTGT AACTCTCTTC AGAGCAAAAA CCACTTAGAA 
GGCTTTAGAA AAGAACTTAA GAATTGGGAA GCAGGCTATA AATCTCATGA GTTCCACCCA 
GAATCACATC TCCAAATAAA AAATAGTTTG ATAAAAAGGT CACATGTTCA TGAAACCAAT 
GGAAAGTTAC TTCCTCCACC CAGTCCACAA ACACTCAAGG ACCATAGTGC AAGAGAAACT 
ATCCATCAGG CAGATGAACA GAAGGCTGAT AAAACAGATG AATGCAGATT TTCTGAGAGG 
CTTAATATAG AAAATTCTGA GAGAAGAGGT TTGCCTTTTC ATGGTGATAA CTCTGCTTCT 
GGGAAGAGAG TGAACAGCAG TGAAATAGTC TTACCATCTT CATTGCGGTC TTCACACATG 
AGAACTGTTG GGTTAAAGCC AGAAATTGCC CCCCTCTTCC AGCAACAAAA TATGATGGAA 
CAATGTTACT CTGAGAACTC TCAGTCCAAG GAACATACAG TTCAGCCAAC CTGCAGATCT 
GATGGGTGTC AGATGCCAAA ACCTTTTTGC CAGAATCTAC CACCCCCGTT GCCACCAAAG 
AAATATGCTG TAACTAGTGT GCTACAGTCA GAGAAAAATG AATCTACAGC TGATGTCAAT 
CTTACAGAGG TGTTTCAAGC TAATTCTTCT AGCCTTCCGA AGCACAGTTT AAGTATAGCT 
TCAGAACCAA ATTTAGAAAT GAGTACATAT GTGAATGATG AAAGGCGGAA AGGAACATTT 
CAAGTGAAAG AATGTGTTGA TAACACATCA GACTACCCAT CCAGCTCTTC AGCTAATGAT 
TTTCAGGCAA ACTCAGGTGC AATTATTGAT GCATGTTGCC AACCAGAACT AGACTCTAGT 
TCTCCCAGTA CAAATGAGAC AATATCATTA ACCACCTATT TTTCAGTTGA TAGCTGCATG 
ACTGACACAT ACAGATTGAA GTATCATCAG AGACCTAAGC TCTGTTTTCC AGAAAGCAGC 
GGCTTTTGTA ATGATAATTC AGTATCTCAG AGTGACAGAG AGGTAGGGCC TGTGTTACAT 
AATAGTCTTG GTTCAGGCTA TCCTTCTGAG CAAAGATGTA AACCTAGCAT ACACTGTAAT 
AGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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USP53 cDNA ORF clone, Otolemur garnettii(small-eared galago)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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