OT13G00110 cDNA ORF clone, Ostreococcus tauri

Gene
Clones
gRNAs

Summary

Gene Symbol	OT13G00110
Entrez Gene ID	9837403
Full Name	DNA topoisomerase, type IIA, subunit A/ C-terminal, alpha-beta
Synonyms	Ot13g00110
Gene Type	protein-coding
Organism	Ostreococcus tauri

mRNA and Protein(s)

mRNA	Protein	Name
XM_022982646.1	XP_022840150.1	DNA topoisomerase, type IIA, subunit A/ C-terminal, alpha-beta
XM_003082434.1	XP_003082482.1	putative DNA topoisomerase II (ISS)

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome of the model marine alga Ostreococcus tauri unfolds by assessing Illumina de novo assemblies.
Blanc-Mathieu R, Verhelst B, Derelle E, Rombauts S, Bouget FY, Carré I, Château A, Eyre-Walker A, Grimsley N, Moreau H, Piégu B, Rivals E, Schackwitz W, Van de Peer Y, Piganeau G
BMC genomics151103(2014 Dec)

Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features.
Derelle E, Ferraz C, Rombauts S, Rouzé P, Worden AZ, Robbens S, Partensky F, Degroeve S, Echeynié S, Cooke R, Saeys Y, Wuyts J, Jabbari K, Bowler C, Panaud O, Piégu B, Ball SG, Ral JP, Bouget FY, Piganeau G, De Baets B, Picard A, Delseny M, Demaille J, Van de Peer Y, Moreau H
Proceedings of the National Academy of Sciences of the United States of America103(31)11647-52(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following OT13G00110 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OT13G00110 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOd05723	XM_003082434.1 Latest version!	Ostreococcus tauri putative DNA topoisomerase II (ISS) (Ot13g00110) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOd77511	XM_022982646.1 Latest version!	Ostreococcus tauri DNA topoisomerase, type IIA, subunit A/ C-terminal, alpha-beta (OT_ostta13g00120), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OT13G00110 ( XM_003082434.1 ) cDNA ORF clone, Ostreococcus tauri -> XP_003082482.1 Ostreococcus tauri putative DNA topoisomerase II (ISS) (Ot13g00110) mRNA, complete cds.

Price & Availability↑

CloneID	OOd05723
Clone ID Related Accession (Same CDS sequence)	XM_003082434.1
Accession Version	XM_003082434.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1373472000000
Organism	Ostreococcus tauri
Product	putative DNA topoisomerase II (ISS)
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_014438). COMPLETENESS: incomplete on both ends.

ATGCTCGTCG ATCGCGTCGA TCGCGTCGAG GGCGCGGAGG CGTACTCGGA CGATGATTTC 
GATGAAAACG CACCGGTCGT GACGCACGGC AAGGGCGAGA AGACGGCGAC CGCGAAGACC 
AAACCGCTGG CGAACGCGAC GAACAAGAAG ACGGTTGAGG AGATTTACCA AAAGAAGACG 
CAGCTCGAGC ACATCTTGTT GAGACCGGAC ACGTACGTCG GATCGTGCGA GCGAGCGGAG 
GAGAAGGCGT GGGTGTACGA CGACGCGACG AAATCTTTGG TGCACAGACA GGTCTCGTTC 
GTTCCGGGTC TTTACAAAAT CTTTGATGAG ATTCTCGTCA ATGCGACGGA TAACAAGGTG 
CGCGACCCAA AGATGAAGGA GCTGCGCGTG GACATCGACG TTGAGCAGGG CATCATCCGT 
GTGTACAACG ATGGTAACGG GATCCCGGTT GAGATGCACA AGGAGGAGGG CGTGTACGTC 
CCGGAGCTCA TTTTCGGTCA TCTCTTGACG TCGAGCAACT ACGACGACAA CGAGAAGAAG 
GTTGTCGGCG GGCGAAACGG GTACGGCGCC AAGTTGGCCA ATATTTTCTC CACAGAGTTC 
ATCATCGAGA CCTGCGATGG CTCTCGCGAG AAGAGATACC GACAAGTGTT CAACAATAAT 
ATGAGCGTCA AGGGCGAGCC GGTGGTGACA AAGTGCAAGA AGAGTGAAAA TTGGACGTGC 
GTCACTTTCA AGCCAGACTT TGCCAAGTTT GGCATGGAGT CTCTCGTCAA CGAAGATGTC 
GCCGCGTTGA TGTGCAAGCG CGTGTACGAT GCGGCGGGTA ACATCGGTAA GAACACGAGC 
ATCTATCTCA ACGGCGAGAA GCTCGCGATC AAGGGCTTCA ACGATTACGT AAGCATGTAC 
TTGAACAGCC AGGCGACGGC GCCGAAGATC TTTGATCTGT CCACGGATCG ATGGCAAGTG 
TGCGTCAGCA TCTCTGACTC GCAGCAGTTT CAGCAGTGCT CGTTCGTGAA CGGTATTTGT 
ACGATGAAGG GCGGCACGCA CGTCAACAAT GTGGCCGATC AAATTTCTCA GAAGCTGTTG 
GAGAAGATTC AGAAGAAGGA GAAGACCTGC AAGAATTTGA AGGCGTTTCA AGTCAAGAAC 
CATCTCTGGG TGTTCGTGAA CGCTCTCATT GAGAACCCGG CGTTTGATTC GCAGACCAAG 
GAGACACTCA TCACGCGACC GAACAAGTTT GGGAGCTCTT ATTCGCCGTC GGATTCCATC 
ATCAAGAAAC TCATGGACTC GGGCGTGGTT GAAAACATCA TGTCTTGGGC GCAGTTCAAG 
CAGAGCAAGG AACTGAAGAA GACTGACGGT GCCAAGCGCT CACGTCTGAC GGGAATCCCG 
AAGCTCGATG ACGCCAACGA TGCCGGTGGA CGAAACTCTG AGTCTTGTAC TTTGATTCTC 
ACCGAAGGGG ATTCTGCCAA GGCGCTCGCT GTGAGCGGTC TCTCCGTCGT TGGCAGAGAC 
AAGTATGGTG TCTTCCCGCT TCGTGGTAAA CTTCTGAACG TGCGCGATGC TTCGCATGAC 
CAAATCAAAA ACAACGCCGA AATCAACAAC ATCAAGCAAA TTCTCGGTCT GCAGCACGGC 
AAGGAGTACG ATAGCGCCAA ATCGTTGCGT TACGGTAAGC TCATGATCAT GACAGACCAA 
GATCATGATG GTTCTCACAT TAAGGGTCTT CTCATCAACT TCTTGCACGC TCACTTCCCA 
TCTCTGCTTA AGATTCCTGG TTTTTTGCTC GAGTTCATCA CGCCGATCAT CAAGGCGACC 
AAGGGGAAGC AGTCGAAGGT GTTCTACACG TTGCCCGAGT ACGAGACTTG GAAGGAGGCG 
AACCAAGGCT CGGCTGGATG GAGCATCAAG TACTACAAGG GTCTTGGTAC GAGCACAGCG 
AATGAAGCCA AGGAGTACTT CGCGTCTCTT GATCACCACC GCAAGAATTT CATCTGGGAA 
AGCGATGGTG ACGGTGACTT GATCGACATG TCGTTCAGCA AGAAGCGTGT CGAGGATCGC 
AAAGCTTGGC TGAATGCGTA CGAGCCAGGC ACATTCTTGG ATATGAGCGG CGAGGCGATT 
CGATACGACG AGTTTATCAA CAAGGAACTC ATTTTGTTCT CTAGAGCTGA CTTGTTACGC 
TCGATTCCGA GCATGGTGGA CGGCTTCAAG CCCTCACAAC GCAAGGTTCT CTTCTCATGC 
TTCAAGCGCA AGCTCAAGTC GGATATCAAA GTCGCGCAGT TGAGCGGCTA TGTCTCGGAG 
CACTCCGCGT ATCATCACGG CGAAGCTTCA CTCGCGATGA CCATCGTCAA CTTGGCGCAA 
GATTTCGTCG GTTCGAACAA CATCAACTTG CTCGTGCCGA GCGGTCAATT TGGTACGCGT 
TTGCAAGGCG GTAAGGATCA CGCAAGCCCA CGTTACATCT TCACTCGCCT GCATGAGATC 
TGCCGTGCGA TTTTCCCGGA GTGCGACGAT GCTCTGCTGA ACTATCTTGA TGAAGATGGA 
CAACGTATCG AGCCGGACTT CTATTATCCG GTGCTTCCGC TCGTGCTTGT GAATGGCGCC 
GAAGGCATCG GTACGGGATG GTCGACGAGC ATTCCTAACT TCAACCCGCG TGACATCATC 
TCCAACATCA AACTCGTGCT TGAGGGCGAG GAAGCGCGTC AGATGCACCC GTGGTATAGA 
AACTTCAACG GCAGCATCGT CGAGGAGATT GTCAAGGGAG ATTCTCGTTA CACTGTCACT 
GGCTCTTACA TTATTCGTGA CGAATGCACG TTGGAAATCA CTGAGCTGCC GCTTCGATCC 
TGGACCACTG ATTACAAGGA TTTCTTGGAA GATATGCTCG CGCCGAAAAC GAAGAACGAA 
AAGCCTTTCA TCACTGACTA CAAGGAGTAC CACACTGACA CTACTGTGCA CTTCGTCATC 
ACGATGCCCC CTGAAAACTT GGCCGCGGCG CAGAGCGCGC CCGGCGGCAT TGAGAAGAAG 
TTCAAGTTGA GCACCAAGGT AAGCATCAGC AACATGCACT TGTTCAATGA ACACGGTGCG 
ATTACCAAGT ACGCCTCACC GATCGACGTT CTGCAAGCGT TCATCCCTCT CCGTCTCAAG 
GCGTACGCGC AACGCAGAGA CATGTTGATT CGTCTCGCTG AGTCTCAGCT CAAACGTCTC 
TCCAACAAGA TGCGTTTCAT TCTCGCCGTT GTCGATGGTA GTTTGGTCAA CTCGATGCTT 
TATGCTATGA CCGACTTCCC AAGACGGACA AGGGCACCAA ACCGCTGGCG CATCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003082482.1
CDS	1..3300
Translation	MLVDRVDRVEGAEAYSDDDFDENAPVVTHGKGEKTATAKTKPLANATNKKTVEEIYQKKTQLEHILLRPDTYVGSCERAEEKAWVYDDATKSLVHRQVSFVPGLYKIFDEILVNATDNKVRDPKMKELRVDIDVEQGIIRVYNDGNGIPVEMHKEEGVYVPELIFGHLLTSSNYDDNEKKVVGGRNGYGAKLANIFSTEFIIETCDGSREKRYRQVFNNNMSVKGEPVVTKCKKSENWTCVTFKPDFAKFGMESLVNEDVAALMCKRVYDAAGNIGKNTSIYLNGEKLAIKGFNDYVSMYLNSQATAPKIFDLSTDRWQVCVSISDSQQFQQCSFVNGICTMKGGTHVNNVADQISQKLLEKIQKKEKTCKNLKAFQVKNHLWVFVNALIENPAFDSQTKETLITRPNKFGSSYSPSDSIIKKLMDSGVVENIMSWAQFKQSKELKKTDGAKRSRLTGIPKLDDANDAGGRNSESCTLILTEGDSAKALAVSGLSVVGRDKYGVFPLRGKLLNVRDASHDQIKNNAEINNIKQILGLQHGKEYDSAKSLRYGKLMIMTDQDHDGSHIKGLLINFLHAHFPSLLKIPGFLLEFITPIIKATKGKQSKVFYTLPEYETWKEANQGSAGWSIKYYKGLGTSTANEAKEYFASLDHHRKNFIWESDGDGDLIDMSFSKKRVEDRKAWLNAYEPGTFLDMSGEAIRYDEFINKELILFSRADLLRSIPSMVDGFKPSQRKVLFSCFKRKLKSDIKVAQLSGYVSEHSAYHHGEASLAMTIVNLAQDFVGSNNINLLVPSGQFGTRLQGGKDHASPRYIFTRLHEICRAIFPECDDALLNYLDEDGQRIEPDFYYPVLPLVLVNGAEGIGTGWSTSIPNFNPRDIISNIKLVLEGEEARQMHPWYRNFNGSIVEEIVKGDSRYTVTGSYIIRDECTLEITELPLRSWTTDYKDFLEDMLAPKTKNEKPFITDYKEYHTDTTVHFVITMPPENLAAAQSAPGGIEKKFKLSTKVSISNMHLFNEHGAITKYASPIDVLQAFIPLRLKAYAQRRDMLIRLAESQLKRLSNKMRFILAVVDGSLVNSMLYAMTDFPRRTRAPNRWRIL

Target ORF information:

RefSeq Version	XM_003082434.1
Organism	Ostreococcus tauri
Definition	Ostreococcus tauri putative DNA topoisomerase II (ISS) (Ot13g00110) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003082434.1

ORF Insert Sequence:

ATGCTCGTCG ATCGCGTCGA TCGCGTCGAG GGCGCGGAGG CGTACTCGGA CGATGATTTC 
GATGAAAACG CACCGGTCGT GACGCACGGC AAGGGCGAGA AGACGGCGAC CGCGAAGACC 
AAACCGCTGG CGAACGCGAC GAACAAGAAG ACGGTTGAGG AGATTTACCA AAAGAAGACG 
CAGCTCGAGC ACATCTTGTT GAGACCGGAC ACGTACGTCG GATCGTGCGA GCGAGCGGAG 
GAGAAGGCGT GGGTGTACGA CGACGCGACG AAATCTTTGG TGCACAGACA GGTCTCGTTC 
GTTCCGGGTC TTTACAAAAT CTTTGATGAG ATTCTCGTCA ATGCGACGGA TAACAAGGTG 
CGCGACCCAA AGATGAAGGA GCTGCGCGTG GACATCGACG TTGAGCAGGG CATCATCCGT 
GTGTACAACG ATGGTAACGG GATCCCGGTT GAGATGCACA AGGAGGAGGG CGTGTACGTC 
CCGGAGCTCA TTTTCGGTCA TCTCTTGACG TCGAGCAACT ACGACGACAA CGAGAAGAAG 
GTTGTCGGCG GGCGAAACGG GTACGGCGCC AAGTTGGCCA ATATTTTCTC CACAGAGTTC 
ATCATCGAGA CCTGCGATGG CTCTCGCGAG AAGAGATACC GACAAGTGTT CAACAATAAT 
ATGAGCGTCA AGGGCGAGCC GGTGGTGACA AAGTGCAAGA AGAGTGAAAA TTGGACGTGC 
GTCACTTTCA AGCCAGACTT TGCCAAGTTT GGCATGGAGT CTCTCGTCAA CGAAGATGTC 
GCCGCGTTGA TGTGCAAGCG CGTGTACGAT GCGGCGGGTA ACATCGGTAA GAACACGAGC 
ATCTATCTCA ACGGCGAGAA GCTCGCGATC AAGGGCTTCA ACGATTACGT AAGCATGTAC 
TTGAACAGCC AGGCGACGGC GCCGAAGATC TTTGATCTGT CCACGGATCG ATGGCAAGTG 
TGCGTCAGCA TCTCTGACTC GCAGCAGTTT CAGCAGTGCT CGTTCGTGAA CGGTATTTGT 
ACGATGAAGG GCGGCACGCA CGTCAACAAT GTGGCCGATC AAATTTCTCA GAAGCTGTTG 
GAGAAGATTC AGAAGAAGGA GAAGACCTGC AAGAATTTGA AGGCGTTTCA AGTCAAGAAC 
CATCTCTGGG TGTTCGTGAA CGCTCTCATT GAGAACCCGG CGTTTGATTC GCAGACCAAG 
GAGACACTCA TCACGCGACC GAACAAGTTT GGGAGCTCTT ATTCGCCGTC GGATTCCATC 
ATCAAGAAAC TCATGGACTC GGGCGTGGTT GAAAACATCA TGTCTTGGGC GCAGTTCAAG 
CAGAGCAAGG AACTGAAGAA GACTGACGGT GCCAAGCGCT CACGTCTGAC GGGAATCCCG 
AAGCTCGATG ACGCCAACGA TGCCGGTGGA CGAAACTCTG AGTCTTGTAC TTTGATTCTC 
ACCGAAGGGG ATTCTGCCAA GGCGCTCGCT GTGAGCGGTC TCTCCGTCGT TGGCAGAGAC 
AAGTATGGTG TCTTCCCGCT TCGTGGTAAA CTTCTGAACG TGCGCGATGC TTCGCATGAC 
CAAATCAAAA ACAACGCCGA AATCAACAAC ATCAAGCAAA TTCTCGGTCT GCAGCACGGC 
AAGGAGTACG ATAGCGCCAA ATCGTTGCGT TACGGTAAGC TCATGATCAT GACAGACCAA 
GATCATGATG GTTCTCACAT TAAGGGTCTT CTCATCAACT TCTTGCACGC TCACTTCCCA 
TCTCTGCTTA AGATTCCTGG TTTTTTGCTC GAGTTCATCA CGCCGATCAT CAAGGCGACC 
AAGGGGAAGC AGTCGAAGGT GTTCTACACG TTGCCCGAGT ACGAGACTTG GAAGGAGGCG 
AACCAAGGCT CGGCTGGATG GAGCATCAAG TACTACAAGG GTCTTGGTAC GAGCACAGCG 
AATGAAGCCA AGGAGTACTT CGCGTCTCTT GATCACCACC GCAAGAATTT CATCTGGGAA 
AGCGATGGTG ACGGTGACTT GATCGACATG TCGTTCAGCA AGAAGCGTGT CGAGGATCGC 
AAAGCTTGGC TGAATGCGTA CGAGCCAGGC ACATTCTTGG ATATGAGCGG CGAGGCGATT 
CGATACGACG AGTTTATCAA CAAGGAACTC ATTTTGTTCT CTAGAGCTGA CTTGTTACGC 
TCGATTCCGA GCATGGTGGA CGGCTTCAAG CCCTCACAAC GCAAGGTTCT CTTCTCATGC 
TTCAAGCGCA AGCTCAAGTC GGATATCAAA GTCGCGCAGT TGAGCGGCTA TGTCTCGGAG 
CACTCCGCGT ATCATCACGG CGAAGCTTCA CTCGCGATGA CCATCGTCAA CTTGGCGCAA 
GATTTCGTCG GTTCGAACAA CATCAACTTG CTCGTGCCGA GCGGTCAATT TGGTACGCGT 
TTGCAAGGCG GTAAGGATCA CGCAAGCCCA CGTTACATCT TCACTCGCCT GCATGAGATC 
TGCCGTGCGA TTTTCCCGGA GTGCGACGAT GCTCTGCTGA ACTATCTTGA TGAAGATGGA 
CAACGTATCG AGCCGGACTT CTATTATCCG GTGCTTCCGC TCGTGCTTGT GAATGGCGCC 
GAAGGCATCG GTACGGGATG GTCGACGAGC ATTCCTAACT TCAACCCGCG TGACATCATC 
TCCAACATCA AACTCGTGCT TGAGGGCGAG GAAGCGCGTC AGATGCACCC GTGGTATAGA 
AACTTCAACG GCAGCATCGT CGAGGAGATT GTCAAGGGAG ATTCTCGTTA CACTGTCACT 
GGCTCTTACA TTATTCGTGA CGAATGCACG TTGGAAATCA CTGAGCTGCC GCTTCGATCC 
TGGACCACTG ATTACAAGGA TTTCTTGGAA GATATGCTCG CGCCGAAAAC GAAGAACGAA 
AAGCCTTTCA TCACTGACTA CAAGGAGTAC CACACTGACA CTACTGTGCA CTTCGTCATC 
ACGATGCCCC CTGAAAACTT GGCCGCGGCG CAGAGCGCGC CCGGCGGCAT TGAGAAGAAG 
TTCAAGTTGA GCACCAAGGT AAGCATCAGC AACATGCACT TGTTCAATGA ACACGGTGCG 
ATTACCAAGT ACGCCTCACC GATCGACGTT CTGCAAGCGT TCATCCCTCT CCGTCTCAAG 
GCGTACGCGC AACGCAGAGA CATGTTGATT CGTCTCGCTG AGTCTCAGCT CAAACGTCTC 
TCCAACAAGA TGCGTTTCAT TCTCGCCGTT GTCGATGGTA GTTTGGTCAA CTCGATGCTT 
TATGCTATGA CCGACTTCCC AAGACGGACA AGGGCACCAA ACCGCTGGCG CATCCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OT13G00110 ( XM_022982646.1 ) cDNA ORF clone, Ostreococcus tauri -> XP_022840150.1 Ostreococcus tauri DNA topoisomerase, type IIA, subunit A/ C-terminal, alpha-beta (OT_ostta13g00120), partial mRNA.

Price & Availability↑

CloneID	OOd77511
Clone ID Related Accession (Same CDS sequence)	XM_022982646.1
Accession Version	XM_022982646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509984000000
Organism	Ostreococcus tauri
Product	DNA topoisomerase, type IIA, subunit A/ C-terminal, alpha-beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_014438). COMPLETENESS: incomplete on both ends.

ATGGCGATGC TCGTCGATCG CGTCGATCGC GTCGAGGGCG CGGAGGCGTA CTCGGACGAT 
GATTTCGATG AAAACGCACC GGTCGTGACG CACGGCAAGG GCGAGAAGAC GGCGACCGCG 
AAGACCAAAC CGCTGGCGAA CGCGACGAAC AAGAAGACGG TTGAGGAGAT TTACCAAAAG 
AAGACGCAGC TCGAGCACAT CTTGTTGAGA CCGGACACGT ACGTCGGATC GTGCGAGCGA 
GCGGAGGAGA AGGCGTGGGT GTACGACGAC GCGACGAAAT CTTTGGTGCA CAGACAGGTC 
TCGTTCGTTC CGGGTCTTTA CAAAATCTTT GATGAGATTC TCGTCAATGC GACGGATAAC 
AAGGTGCGCG ACCCAAAGAT GAAGGAGCTG CGCGTGGACA TCGACGTTGA GCAGGGCATC 
ATCCGTGTGT ACAACGATGG TAACGGGATC CCGGTTGAGA TGCACAAGGA GGAGGGCGTG 
TACGTCCCGG AGCTCATTTT CGGTCATCTC TTGACGTCGA GCAACTACGA CGACAACGAG 
AAGAAGGTTG TCGGCGGGCG AAACGGGTAC GGCGCCAAGT TGGCCAATAT TTTCTCCACA 
GAGTTCATCA TCGAGACCTG CGATGGCTCT CGCGAGAAGA GATACCGACA AGTGTTCAAC 
AATAATATGA GCGTCAAGGG CGAGCCGGTG GTGACAAAGT GCAAGAAGAG TGAAAATTGG 
ACGTGCGTCA CTTTCAAGCC AGACTTTGCC AAGTTTGGCA TGGAGTCTCT CGTCAACGAA 
GATGTCGCCG CGTTGATGTG CAAGCGCGTG TACGATGCGG CGGGTAACAT CGGTAAGAAC 
ACGAGCATCT ATCTCAACGG CGAGAAGCTC GCGATCAAGG GCTTCAACGA TTACGTAAGC 
ATGTACTTGA ACAGCCAGGC GACGGCGCCG AAGATCTTTG ATCTGTCCAC GGATCGATGG 
CAAGTGTGCG TCAGCATCTC TGACTCGCAG CAGTTTCAGC AGTGCTCGTT CGTGAACGGT 
ATTTGTACGA TGAAGGGCGG CACGCACGTC AACAATGTGG CCGATCAAAT TTCTCAGAAG 
CTGTTGGAGA AGATTCAGAA GAAGGAGAAG ACCTGCAAGA ATTTGAAGGC GTTTCAAGTC 
AAGAACCATC TCTGGGTGTT CGTGAACGCT CTCATTGAGA ACCCGGCGTT TGATTCGCAG 
ACCAAGGAGA CACTCATCAC GCGACCGAAC AAGTTTGGGA GCTCTTATTC GCCGTCGGAT 
TCCATCATCA AGAAACTCAT GGACTCGGGC GTGGTTGAAA ACATCATGTC TTGGGCGCAG 
TTCAAGCAGA GCAAGGAACT GAAGAAGACT GACGGTGCCA AGCGCTCACG TCTGACGGGA 
ATCCCGAAGC TCGATGACGC CAACGATGCC GGTGGACGAA ACTCTGAGTC TTGTACTTTG 
ATTCTCACCG AAGGGGATTC TGCCAAGGCG CTCGCTGTGA GCGGTCTCTC CGTCGTTGGC 
AGAGACAAGT ATGGTGTCTT CCCGCTTCGT GGTAAACTTC TGAACGTGCG CGATGCTTCG 
CATGACCAAA TCAAAAACAA CGCCGAAATC AACAACATCA AGCAAATTCT CGGTCTGCAG 
CACGGCAAGG AGTACGATAG CGCCAAATCG TTGCGTTACG GTAAGCTCAT GATCATGACA 
GACCAAGATC ATGATGGTTC TCACATTAAG GGTCTTCTCA TCAACTTCTT GCACGCTCAC 
TTCCCATCTC TGCTTAAGAT TCCTGGTTTT TTGCTCGAGT TCATCACGCC GATCATCAAG 
GCGACCAAGG GGAAGCAGTC GAAGGTGTTC TACACGTTGC CCGAGTACGA GACTTGGAAG 
GAGGCGAACC AAGGCTCGGC TGGATGGAGC ATCAAGTACT ACAAGGGTCT TGGTACGAGC 
ACAGCGAATG AAGCCAAGGA GTACTTCGCG TCTCTTGATC ACCACCGCAA GAATTTCATC 
TGGGAAAGCG ATGGTGACGG TGACTTGATC GACATGTCGT TCAGCAAGAA GCGTGTCGAG 
GATCGCAAAG CTTGGCTGAA TGCGTACGAG CCAGGCACAT TCTTGGATAT GAGCGGCGAG 
GCGATTCGAT ACGACGAGTT TATCAACAAG GAACTCATTT TGTTCTCTAG AGCTGACTTG 
TTACGCTCGA TTCCGAGCAT GGTGGACGGC TTCAAGCCCT CACAACGCAA GGTTCTCTTC 
TCATGCTTCA AGCGCAAGCT CAAGTCGGAT ATCAAAGTCG CGCAGTTGAG CGGCTATGTC 
TCGGAGCACT CCGCGTATCA TCACGGCGAA GCTTCACTCG CGATGACCAT CGTCAACTTG 
GCGCAAGATT TCGTCGGTTC GAACAACATC AACTTGCTCG TGCCGAGCGG TCAATTTGGT 
ACGCGTTTGC AAGGCGGTAA GGATCACGCA AGCCCACGTT ACATCTTCAC TCGCCTGCAT 
GAGATCTGCC GTGCGATTTT CCCGGAGTGC GACGATGCTC TGCTGAACTA TCTTGATGAA 
GATGGACAAC GTATCGAGCC GGACTTCTAT TATCCGGTGC TTCCGCTCGT GCTTGTGAAT 
GGCGCCGAAG GCATCGGTAC GGGATGGTCG ACGAGCATTC CTAACTTCAA CCCGCGTGAC 
ATCATCTCCA ACATCAAACT CGTGCTTGAG GGCGAGGAAG CGCGTCAGAT GCACCCGTGG 
TATAGAAACT TCAACGGCAG CATCGTCGAG GAGATTGTCA AGGGAGATTC TCGTTACACT 
GTCACTGGCT CTTACATTAT TCGTGACGAA TGCACGTTGG AAATCACTGA GCTGCCGCTT 
CGATCCTGGA CCACTGATTA CAAGGATTTC TTGGAAGATA TGCTCGCGCC GAAAACGAAG 
AACGAAAAGC CTTTCATCAC TGACTACAAG GAGTACCACA CTGACACTAC TGTGCACTTC 
GTCATCACGA TGCCCCCTGA AAACTTGGCC GCGGCGCAGA GCGCGCCCGG CGGCATTGAG 
AAGAAGTTCA AGTTGAGCAC CAAGGTAAGC ATCAGCAACA TGCACTTGTT CAATGAACAC 
GGTGCGATTA CCAAGTACGC CTCACCGATC GACGTTCTGC AAGCGTTCAT CCCTCTCCGT 
CTCAAGGCGT ACGCGCAACG CAGAGACATG TTGATTCGTC TCGCTGAGTC TCAGCTCAAA 
CGTCTCTCCA ACAAGATGCG TTTCATTCTC GCCGTTGTCG ATGGTAGTTT GGTCGTCAAT 
CGTAAGAAAA AGCAGGAACT CATCGAAGAA CTCGATGCTT TATGCTATGA CCGACTTCCC 
AAGACGGACA AGGGCACCGT TGCTGAACTT GACGAAGACG GTCAGTTCAT TCCGGAAGAA 
ACCGCTGGCG CATCCTTTGA TTACCTGCTC GGCATGCCGT TATGGAATTT GACGCTCGAA 
AAAGTGGAAG AGCTGGTGTC CGAGAAAGAG GGCAAAGAAG CTGAGGTCGC CGAACTTCAC 
AAGACGACCG ATAAGGATCT GTGGATTCGG GATCTGGAGA ACCTCGAGCA GAAACTCGAT 
CTCCTCGAAG CCGAAGACGA AGAGGCTCGC CAGGTTCTTG AAAGGCAGCA ACGTGCGGCC 
GCGCGCAACA ACACTACTGC GGGTAAGAAG GCGGCAAAGA AGAAGCTTGC CAAGCAACGT 
GCAGCGAACA GCAGCGACGA AGATGAGTGG GATGATGACT TCAGCGATGA TGACTTTGAA 
ATCTCGAAGC CAAAGAAGAG GGCTGCGCCC AAACCGAAGG CGGCGCCCAA ACCGAAGGCG 
GCGCCTGCGC CCGTGGCCGC GCCCGTTCTG GAACCGCCGA AGGATGAAAG CGACGCTGAG 
GAAACGGAAC CGATGTCTCT CGCCGAGCGC TTGGCTTTGC GAGGAGGCGC AGCGCTGAGC 
AAGCCCGCCA AATCGTCTAC CGCAGCATTC GATATGTCCT CCATGAGCAC GGCGGTGACG 
GCTGCCGCTG CGAAGAAACC TGCCGCTGCG AAGAAGCCTG CCGCTGCGAA GAAACCGGCT 
GCTGCGAAGA AGCCTGCGCC CGCAAAGAAG GCTATGTCTG ATTCTGAAGA TTTTGACGAC 
GAGAGCGATT CTGAAGTGGT CGCTCCAGTG GCGCCGAGAT CTACTGCTCG CCCGCAACGA 
GCACGCCCGG TCAAGAAGTA CGCAGTTTCG GAATCCGAAG AAGAGTCTGA AGAAGATTTC 
TCTGAAGAGG ATGACGAAAG CGACTTCAGC GAATCTGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022840150.1
CDS	1..4242
Translation	MAMLVDRVDRVEGAEAYSDDDFDENAPVVTHGKGEKTATAKTKPLANATNKKTVEEIYQKKTQLEHILLRPDTYVGSCERAEEKAWVYDDATKSLVHRQVSFVPGLYKIFDEILVNATDNKVRDPKMKELRVDIDVEQGIIRVYNDGNGIPVEMHKEEGVYVPELIFGHLLTSSNYDDNEKKVVGGRNGYGAKLANIFSTEFIIETCDGSREKRYRQVFNNNMSVKGEPVVTKCKKSENWTCVTFKPDFAKFGMESLVNEDVAALMCKRVYDAAGNIGKNTSIYLNGEKLAIKGFNDYVSMYLNSQATAPKIFDLSTDRWQVCVSISDSQQFQQCSFVNGICTMKGGTHVNNVADQISQKLLEKIQKKEKTCKNLKAFQVKNHLWVFVNALIENPAFDSQTKETLITRPNKFGSSYSPSDSIIKKLMDSGVVENIMSWAQFKQSKELKKTDGAKRSRLTGIPKLDDANDAGGRNSESCTLILTEGDSAKALAVSGLSVVGRDKYGVFPLRGKLLNVRDASHDQIKNNAEINNIKQILGLQHGKEYDSAKSLRYGKLMIMTDQDHDGSHIKGLLINFLHAHFPSLLKIPGFLLEFITPIIKATKGKQSKVFYTLPEYETWKEANQGSAGWSIKYYKGLGTSTANEAKEYFASLDHHRKNFIWESDGDGDLIDMSFSKKRVEDRKAWLNAYEPGTFLDMSGEAIRYDEFINKELILFSRADLLRSIPSMVDGFKPSQRKVLFSCFKRKLKSDIKVAQLSGYVSEHSAYHHGEASLAMTIVNLAQDFVGSNNINLLVPSGQFGTRLQGGKDHASPRYIFTRLHEICRAIFPECDDALLNYLDEDGQRIEPDFYYPVLPLVLVNGAEGIGTGWSTSIPNFNPRDIISNIKLVLEGEEARQMHPWYRNFNGSIVEEIVKGDSRYTVTGSYIIRDECTLEITELPLRSWTTDYKDFLEDMLAPKTKNEKPFITDYKEYHTDTTVHFVITMPPENLAAAQSAPGGIEKKFKLSTKVSISNMHLFNEHGAITKYASPIDVLQAFIPLRLKAYAQRRDMLIRLAESQLKRLSNKMRFILAVVDGSLVVNRKKKQELIEELDALCYDRLPKTDKGTVAELDEDGQFIPEETAGASFDYLLGMPLWNLTLEKVEELVSEKEGKEAEVAELHKTTDKDLWIRDLENLEQKLDLLEAEDEEARQVLERQQRAAARNNTTAGKKAAKKKLAKQRAANSSDEDEWDDDFSDDDFEISKPKKRAAPKPKAAPKPKAAPAPVAAPVLEPPKDESDAEETEPMSLAERLALRGGAALSKPAKSSTAAFDMSSMSTAVTAAAAKKPAAAKKPAAAKKPAAAKKPAPAKKAMSDSEDFDDESDSEVVAPVAPRSTARPQRARPVKKYAVSESEEESEEDFSEEDDESDFSESE

Target ORF information:

RefSeq Version	XM_022982646.1
Organism	Ostreococcus tauri
Definition	Ostreococcus tauri DNA topoisomerase, type IIA, subunit A/ C-terminal, alpha-beta (OT_ostta13g00120), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022982646.1

ORF Insert Sequence:

ATGGCGATGC TCGTCGATCG CGTCGATCGC GTCGAGGGCG CGGAGGCGTA CTCGGACGAT 
GATTTCGATG AAAACGCACC GGTCGTGACG CACGGCAAGG GCGAGAAGAC GGCGACCGCG 
AAGACCAAAC CGCTGGCGAA CGCGACGAAC AAGAAGACGG TTGAGGAGAT TTACCAAAAG 
AAGACGCAGC TCGAGCACAT CTTGTTGAGA CCGGACACGT ACGTCGGATC GTGCGAGCGA 
GCGGAGGAGA AGGCGTGGGT GTACGACGAC GCGACGAAAT CTTTGGTGCA CAGACAGGTC 
TCGTTCGTTC CGGGTCTTTA CAAAATCTTT GATGAGATTC TCGTCAATGC GACGGATAAC 
AAGGTGCGCG ACCCAAAGAT GAAGGAGCTG CGCGTGGACA TCGACGTTGA GCAGGGCATC 
ATCCGTGTGT ACAACGATGG TAACGGGATC CCGGTTGAGA TGCACAAGGA GGAGGGCGTG 
TACGTCCCGG AGCTCATTTT CGGTCATCTC TTGACGTCGA GCAACTACGA CGACAACGAG 
AAGAAGGTTG TCGGCGGGCG AAACGGGTAC GGCGCCAAGT TGGCCAATAT TTTCTCCACA 
GAGTTCATCA TCGAGACCTG CGATGGCTCT CGCGAGAAGA GATACCGACA AGTGTTCAAC 
AATAATATGA GCGTCAAGGG CGAGCCGGTG GTGACAAAGT GCAAGAAGAG TGAAAATTGG 
ACGTGCGTCA CTTTCAAGCC AGACTTTGCC AAGTTTGGCA TGGAGTCTCT CGTCAACGAA 
GATGTCGCCG CGTTGATGTG CAAGCGCGTG TACGATGCGG CGGGTAACAT CGGTAAGAAC 
ACGAGCATCT ATCTCAACGG CGAGAAGCTC GCGATCAAGG GCTTCAACGA TTACGTAAGC 
ATGTACTTGA ACAGCCAGGC GACGGCGCCG AAGATCTTTG ATCTGTCCAC GGATCGATGG 
CAAGTGTGCG TCAGCATCTC TGACTCGCAG CAGTTTCAGC AGTGCTCGTT CGTGAACGGT 
ATTTGTACGA TGAAGGGCGG CACGCACGTC AACAATGTGG CCGATCAAAT TTCTCAGAAG 
CTGTTGGAGA AGATTCAGAA GAAGGAGAAG ACCTGCAAGA ATTTGAAGGC GTTTCAAGTC 
AAGAACCATC TCTGGGTGTT CGTGAACGCT CTCATTGAGA ACCCGGCGTT TGATTCGCAG 
ACCAAGGAGA CACTCATCAC GCGACCGAAC AAGTTTGGGA GCTCTTATTC GCCGTCGGAT 
TCCATCATCA AGAAACTCAT GGACTCGGGC GTGGTTGAAA ACATCATGTC TTGGGCGCAG 
TTCAAGCAGA GCAAGGAACT GAAGAAGACT GACGGTGCCA AGCGCTCACG TCTGACGGGA 
ATCCCGAAGC TCGATGACGC CAACGATGCC GGTGGACGAA ACTCTGAGTC TTGTACTTTG 
ATTCTCACCG AAGGGGATTC TGCCAAGGCG CTCGCTGTGA GCGGTCTCTC CGTCGTTGGC 
AGAGACAAGT ATGGTGTCTT CCCGCTTCGT GGTAAACTTC TGAACGTGCG CGATGCTTCG 
CATGACCAAA TCAAAAACAA CGCCGAAATC AACAACATCA AGCAAATTCT CGGTCTGCAG 
CACGGCAAGG AGTACGATAG CGCCAAATCG TTGCGTTACG GTAAGCTCAT GATCATGACA 
GACCAAGATC ATGATGGTTC TCACATTAAG GGTCTTCTCA TCAACTTCTT GCACGCTCAC 
TTCCCATCTC TGCTTAAGAT TCCTGGTTTT TTGCTCGAGT TCATCACGCC GATCATCAAG 
GCGACCAAGG GGAAGCAGTC GAAGGTGTTC TACACGTTGC CCGAGTACGA GACTTGGAAG 
GAGGCGAACC AAGGCTCGGC TGGATGGAGC ATCAAGTACT ACAAGGGTCT TGGTACGAGC 
ACAGCGAATG AAGCCAAGGA GTACTTCGCG TCTCTTGATC ACCACCGCAA GAATTTCATC 
TGGGAAAGCG ATGGTGACGG TGACTTGATC GACATGTCGT TCAGCAAGAA GCGTGTCGAG 
GATCGCAAAG CTTGGCTGAA TGCGTACGAG CCAGGCACAT TCTTGGATAT GAGCGGCGAG 
GCGATTCGAT ACGACGAGTT TATCAACAAG GAACTCATTT TGTTCTCTAG AGCTGACTTG 
TTACGCTCGA TTCCGAGCAT GGTGGACGGC TTCAAGCCCT CACAACGCAA GGTTCTCTTC 
TCATGCTTCA AGCGCAAGCT CAAGTCGGAT ATCAAAGTCG CGCAGTTGAG CGGCTATGTC 
TCGGAGCACT CCGCGTATCA TCACGGCGAA GCTTCACTCG CGATGACCAT CGTCAACTTG 
GCGCAAGATT TCGTCGGTTC GAACAACATC AACTTGCTCG TGCCGAGCGG TCAATTTGGT 
ACGCGTTTGC AAGGCGGTAA GGATCACGCA AGCCCACGTT ACATCTTCAC TCGCCTGCAT 
GAGATCTGCC GTGCGATTTT CCCGGAGTGC GACGATGCTC TGCTGAACTA TCTTGATGAA 
GATGGACAAC GTATCGAGCC GGACTTCTAT TATCCGGTGC TTCCGCTCGT GCTTGTGAAT 
GGCGCCGAAG GCATCGGTAC GGGATGGTCG ACGAGCATTC CTAACTTCAA CCCGCGTGAC 
ATCATCTCCA ACATCAAACT CGTGCTTGAG GGCGAGGAAG CGCGTCAGAT GCACCCGTGG 
TATAGAAACT TCAACGGCAG CATCGTCGAG GAGATTGTCA AGGGAGATTC TCGTTACACT 
GTCACTGGCT CTTACATTAT TCGTGACGAA TGCACGTTGG AAATCACTGA GCTGCCGCTT 
CGATCCTGGA CCACTGATTA CAAGGATTTC TTGGAAGATA TGCTCGCGCC GAAAACGAAG 
AACGAAAAGC CTTTCATCAC TGACTACAAG GAGTACCACA CTGACACTAC TGTGCACTTC 
GTCATCACGA TGCCCCCTGA AAACTTGGCC GCGGCGCAGA GCGCGCCCGG CGGCATTGAG 
AAGAAGTTCA AGTTGAGCAC CAAGGTAAGC ATCAGCAACA TGCACTTGTT CAATGAACAC 
GGTGCGATTA CCAAGTACGC CTCACCGATC GACGTTCTGC AAGCGTTCAT CCCTCTCCGT 
CTCAAGGCGT ACGCGCAACG CAGAGACATG TTGATTCGTC TCGCTGAGTC TCAGCTCAAA 
CGTCTCTCCA ACAAGATGCG TTTCATTCTC GCCGTTGTCG ATGGTAGTTT GGTCGTCAAT 
CGTAAGAAAA AGCAGGAACT CATCGAAGAA CTCGATGCTT TATGCTATGA CCGACTTCCC 
AAGACGGACA AGGGCACCGT TGCTGAACTT GACGAAGACG GTCAGTTCAT TCCGGAAGAA 
ACCGCTGGCG CATCCTTTGA TTACCTGCTC GGCATGCCGT TATGGAATTT GACGCTCGAA 
AAAGTGGAAG AGCTGGTGTC CGAGAAAGAG GGCAAAGAAG CTGAGGTCGC CGAACTTCAC 
AAGACGACCG ATAAGGATCT GTGGATTCGG GATCTGGAGA ACCTCGAGCA GAAACTCGAT 
CTCCTCGAAG CCGAAGACGA AGAGGCTCGC CAGGTTCTTG AAAGGCAGCA ACGTGCGGCC 
GCGCGCAACA ACACTACTGC GGGTAAGAAG GCGGCAAAGA AGAAGCTTGC CAAGCAACGT 
GCAGCGAACA GCAGCGACGA AGATGAGTGG GATGATGACT TCAGCGATGA TGACTTTGAA 
ATCTCGAAGC CAAAGAAGAG GGCTGCGCCC AAACCGAAGG CGGCGCCCAA ACCGAAGGCG 
GCGCCTGCGC CCGTGGCCGC GCCCGTTCTG GAACCGCCGA AGGATGAAAG CGACGCTGAG 
GAAACGGAAC CGATGTCTCT CGCCGAGCGC TTGGCTTTGC GAGGAGGCGC AGCGCTGAGC 
AAGCCCGCCA AATCGTCTAC CGCAGCATTC GATATGTCCT CCATGAGCAC GGCGGTGACG 
GCTGCCGCTG CGAAGAAACC TGCCGCTGCG AAGAAGCCTG CCGCTGCGAA GAAACCGGCT 
GCTGCGAAGA AGCCTGCGCC CGCAAAGAAG GCTATGTCTG ATTCTGAAGA TTTTGACGAC 
GAGAGCGATT CTGAAGTGGT CGCTCCAGTG GCGCCGAGAT CTACTGCTCG CCCGCAACGA 
GCACGCCCGG TCAAGAAGTA CGCAGTTTCG GAATCCGAAG AAGAGTCTGA AGAAGATTTC 
TCTGAAGAGG ATGACGAAAG CGACTTCAGC GAATCTGAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome of the model marine alga Ostreococcus tauri unfolds by assessing Illumina de novo assemblies.
BMC genomics151103(2014 Dec)
Blanc-Mathieu R,Verhelst B,Derelle E,Rombauts S,Bouget FY,Carré I,Château A,Eyre-Walker A,Grimsley N,Moreau H,Piégu B,Rivals E,Schackwitz W,Van de Peer Y,Piganeau G

Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features.
Proceedings of the National Academy of Sciences of the United States of America103(31)11647-52(2006 Aug)
Derelle E,Ferraz C,Rombauts S,Rouzé P,Worden AZ,Robbens S,Partensky F,Degroeve S,Echeynié S,Cooke R,Saeys Y,Wuyts J,Jabbari K,Bowler C,Panaud O,Piégu B,Ball SG,Ral JP,Bouget FY,Piganeau G,De Baets B,Picard A,Delseny M,Demaille J,Van de Peer Y,Moreau H

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OT13G00110 cDNA ORF clone, Ostreococcus tauri

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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