OT_ostta04g00400 cDNA ORF clone, Ostreococcus tauri

Gene
Clones
gRNAs

Summary

Gene Symbol	OT_ostta04g00400
Entrez Gene ID	9834173
Full Name	Isoleucine-tRNA ligase, type 1
Synonyms	Ot04g00470
Gene Type	protein-coding
Organism	Ostreococcus tauri

mRNA and Protein(s)

mRNA	Protein	Name
XM_003078517.1	XP_003078565.1	isoleucyl-tRNA synthetase (ISS)
XM_003078517.2	XP_003078565.2	Isoleucine-tRNA ligase, type 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome of the model marine alga Ostreococcus tauri unfolds by assessing Illumina de novo assemblies.
Blanc-Mathieu R, Verhelst B, Derelle E, Rombauts S, Bouget FY, Carré I, Château A, Eyre-Walker A, Grimsley N, Moreau H, Piégu B, Rivals E, Schackwitz W, Van de Peer Y, Piganeau G
BMC genomics151103(2014 Dec)

Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features.
Derelle E, Ferraz C, Rombauts S, Rouzé P, Worden AZ, Robbens S, Partensky F, Degroeve S, Echeynié S, Cooke R, Saeys Y, Wuyts J, Jabbari K, Bowler C, Panaud O, Piégu B, Ball SG, Ral JP, Bouget FY, Piganeau G, De Baets B, Picard A, Delseny M, Demaille J, Van de Peer Y, Moreau H
Proceedings of the National Academy of Sciences of the United States of America103(31)11647-52(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following OT_ostta04g00400 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OT_ostta04g00400 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOd02997	XM_003078517.1 Latest version!	Ostreococcus tauri isoleucyl-tRNA synthetase (ISS) (Ot04g00470) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OOd74722	XM_003078517.2 Latest version!	Ostreococcus tauri Isoleucine-tRNA ligase, type 1 (OT_ostta04g00400), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OT_ostta04g00400 ( XM_003078517.1 ) cDNA ORF clone, Ostreococcus tauri -> XP_003078565.1 Ostreococcus tauri isoleucyl-tRNA synthetase (ISS) (Ot04g00470) mRNA, complete cds.

Price & Availability↑

CloneID	OOd02997
Clone ID Related Accession (Same CDS sequence)	XM_003078517.1
Accession Version	XM_003078517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1373472000000
Organism	Ostreococcus tauri
Product	isoleucyl-tRNA synthetase (ISS)
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_014429). COMPLETENESS: incomplete on both ends.

ATGTCGTCCA CGCGCGCGCG CGCGCGCGCG GTGATCGCAC GACGGGCGCG AGGCGGGACG 
ACGCTCGGTC GAACGGCGAG CGCGAGCGCG ACGCGACGCG GGCGAGGGAC GATCGCGCGC 
GCGAGCGGGG GGGAGAAGAA GGAGAAGAAT GCGTACGGTG AGACGGTGCG GTTGCCGAAG 
ACGAGTTTTG AGATGCGGGC GAACAGCGTG GTGAAGGAGC CGAAGATGCA GGAGTGGTGG 
CGGGAGCGCG GGGTGTACGA AACGCTGAGA GATCGAGACG GTGAGGCGTT CGTGCTGCAC 
GATGGACCGC CGTACGCGAA CGGGGATCTA CACATTGGAC ACGCGCTGAA TAAGATTTTG 
AAGGATTTCG TGAACCGGTG GGAGATGATG AATGGGAAAA AGGTGCGCTT CGTCCCTGGG 
TGGGACTGTC ACGGACTCCC GATCGAGCTC AAGGTGTTGC AGAGCATGGA CGCGGAGGCG 
CGAAAGAATT TGACGCCGAT CAAGCTTCGT TACAAGGCGA AGGCGTTCGC GGAGAAGACG 
GTGAAGGCGC AGCGCGAGCA GTTCAAGCGA TACGGCGTGT GGGCCGACTG GGAGGATCCC 
TACATGACGC TCCTGCCAGA GTACGAGGCG GCGCAGATGG AAGTTTTCGG TAGCATGTTC 
CTGAACGGAC ATATTTACCG CGGGAAGAAG CCCGTGCACT GGTCTCCGTC GAGCATGACG 
GCGCTCGCCG AGGCTGAGCT CGAATACCCC GAGGGACACT TCTCGCAGAG CATCTACGTC 
GCGTTTCCGG TCTCCGAAGT TCCAGATTCG ACGCCGAGCG CGCTGAGGGA TCTGCTCAAG 
GACGCATCTC TGGCTATTTG GACAACGACG CCGTGGACGA TGCCGGCGAA CGCGGCGGTG 
GCGGTAAATG CAAAGTTGGA CTACTCCGTG TGCAGAGTGG AATCGACGGG CAAAACGCTC 
GTCGTCGCCG AAGGTTTGCG CGAAGCCGTC GCGGCGAAGT TAGAGTCCGA GCTCACTACT 
CTTGGAACAT TCAAGGGTCA AGATTTCGAG AACGTGCAGT ACTCACATCC GTTCTACGAA 
CGCAAGTCGC CCGTCATCAT CGGCGGCGAG TACATCACCA CCGAAACTGG TACCGGTTTG 
GTTCACACCG CTCCCGGTCA CGGTCAGGAT GATTACATCT CGGGCATGAA GTACGGGTTG 
CCGTTGTATT CGCCGGTTGA TAACGCCGGT TTGTTCACCG CCGAGGCTGG TGGAGATTTG 
GAGGGAAAGG ATGTGTTGGG CGACGGCAAC GTGCTGTGCA TTGAAAAGTT AGAAACAGCT 
GGGGCGCTTC TCAAGCGCGA GAAGTACGGA CACAAATACC CGTACGACTG GCGCACGAAG 
AAGCCTACGA TCTTCCGCGC GACCGAGCAA TGGTTTGCGT CTGTCGAGGG CTTCCGTGAG 
GACGCTCTCA AGGAGTTGGA CAAGTTGCAG TTTATTCCTG AGTCCGGCTC GAAGAGAATG 
CGACCGATGG TTAGTGGGCG CGCTGACTGG TGCATCTCTC GTCAGCGAGC GTGGGGCGTA 
CCGATTCCGG CGTTTTACCA CAAAGACACG AACGAGGTGC TCATGGACGA AGCCATCATC 
AAACACGTCA CAGAGATCGT TCGCGCGAAG GGTACGAACG CGTGGTGGGA GTTGGAGGTC 
GAGGATCTCT TGCCCGAGGA GCACAAGGCC AAGGCGGGTG AGTACGTTCG CGGTACCGAT 
ACTATGGATG TGTGGTTTGA CTCCGGAAGC TCTTGGGCTG GTGTGGTGAA GACCCGCGGT 
ATGTCGTTTC CCGCCGACGT GTACCTGGAG GGCAGCGATC AGCACAGAGG TTGGTTCCAA 
TCGAGTCTTC TCACCGGCGT CGGTGCGATG GGTCAGGCGC CATACAAGAC GATTTTGACG 
CACGGCTTCG TTCTGGATGA GAAAGGATAC AAGATGTCCA AGTCTTTGGG TAACGTTATC 
GACCCTCGCA CGGTGATCGA GGGCGGAAAG AACCAAAAGC AGGAGCCAGG CTACGGCGCC 
GATACGCTTC GCCTGTGGGT TGCGAGCACG GACTATTCCG GAGACGTGAG TATTGGTATG 
AACATCATCA AACAAACGAG CGAGGCGTAT CGCAAGCTCA GAGGGACGAT TCGATTCGCG 
ATGGGCGTTC TGGATGATTT CAATCCGGCG CAGCAAGTGC CGTACGATGA TTTGCCAGTG 
TTCGACAAGT ACGTCCTTCA TCGCTTGAAC GCGACGGTGA AAGAGATTGA GAGCTCCTAC 
AAGGCGTTCG ACTACAGCCG AGTGGTCTCG GCGGTGACCT CTTTCACGAC ATTCCTTTCC 
AACGTCTACT TGGACGTCAG CAAGGATAAG TTGTACATTG GCGCACAAGA TGACTTCAAG 
CGACGAGCAT GTCAGACCGT AGTGTCTGCC TTGGTCGAGC GTATTATCGC TGCAATCGCA 
CCGCTTACGC CGCATATGGC AGAGGAGGCG TTCCAAGCGC TGCCGTACGA TAAGCCCAAC 
GGAGCAATTT CCGTTTTCAT CGCCGGTTGG CCGTCCACCC CGGCGGCCTG GTCCGAGCTC 
GACGCCAAGG ACGTGGAGTT CTGGGACTCA TTCTTAGAGA TCCGCGACGG CGTGAACAAG 
GTGCTCGAAG AAGCTCGTAC TGCCAAGCTT CTCGGAGCGT CTCTCGAGTC GAAGGTTACT 
ATGCACTGCT CTGACGCCAA ATTCCTGGCT AAGCTCACTG GCGAAGGCAT TGCCGAGGAG 
TTGAGATACC TCTTCATCGT TAGCGAAGTC CAGCTCGCTG ATTCTAGCGA TGTCGCAATC 
GCCGGGTGTG ACTTCAAGTC CGTCGTTGAC GTTCCCGGCG CCGGTACCGT GTCCATCGGT 
GTCGCTCGTG CCGCTGGCTC CAAGTGCGCT CGATGCTGGA ACTTCTCCCC TCTCGTCGGC 
TCCGACGCCA AGCACTCGCA GCTGTGCGAG CGGTGCGTCC CGATCGTGAA CGCCACGCAC 
CCGGATCTCG TCGTCCCTGA GCCCGCCGCC GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003078565.1
CDS	1..3036
Translation	MSSTRARARAVIARRARGGTTLGRTASASATRRGRGTIARASGGEKKEKNAYGETVRLPKTSFEMRANSVVKEPKMQEWWRERGVYETLRDRDGEAFVLHDGPPYANGDLHIGHALNKILKDFVNRWEMMNGKKVRFVPGWDCHGLPIELKVLQSMDAEARKNLTPIKLRYKAKAFAEKTVKAQREQFKRYGVWADWEDPYMTLLPEYEAAQMEVFGSMFLNGHIYRGKKPVHWSPSSMTALAEAELEYPEGHFSQSIYVAFPVSEVPDSTPSALRDLLKDASLAIWTTTPWTMPANAAVAVNAKLDYSVCRVESTGKTLVVAEGLREAVAAKLESELTTLGTFKGQDFENVQYSHPFYERKSPVIIGGEYITTETGTGLVHTAPGHGQDDYISGMKYGLPLYSPVDNAGLFTAEAGGDLEGKDVLGDGNVLCIEKLETAGALLKREKYGHKYPYDWRTKKPTIFRATEQWFASVEGFREDALKELDKLQFIPESGSKRMRPMVSGRADWCISRQRAWGVPIPAFYHKDTNEVLMDEAIIKHVTEIVRAKGTNAWWELEVEDLLPEEHKAKAGEYVRGTDTMDVWFDSGSSWAGVVKTRGMSFPADVYLEGSDQHRGWFQSSLLTGVGAMGQAPYKTILTHGFVLDEKGYKMSKSLGNVIDPRTVIEGGKNQKQEPGYGADTLRLWVASTDYSGDVSIGMNIIKQTSEAYRKLRGTIRFAMGVLDDFNPAQQVPYDDLPVFDKYVLHRLNATVKEIESSYKAFDYSRVVSAVTSFTTFLSNVYLDVSKDKLYIGAQDDFKRRACQTVVSALVERIIAAIAPLTPHMAEEAFQALPYDKPNGAISVFIAGWPSTPAAWSELDAKDVEFWDSFLEIRDGVNKVLEEARTAKLLGASLESKVTMHCSDAKFLAKLTGEGIAEELRYLFIVSEVQLADSSDVAIAGCDFKSVVDVPGAGTVSIGVARAAGSKCARCWNFSPLVGSDAKHSQLCERCVPIVNATHPDLVVPEPAAA

Target ORF information:

RefSeq Version	XM_003078517.1
Organism	Ostreococcus tauri
Definition	Ostreococcus tauri isoleucyl-tRNA synthetase (ISS) (Ot04g00470) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003078517.1

ORF Insert Sequence:

ATGTCGTCCA CGCGCGCGCG CGCGCGCGCG GTGATCGCAC GACGGGCGCG AGGCGGGACG 
ACGCTCGGTC GAACGGCGAG CGCGAGCGCG ACGCGACGCG GGCGAGGGAC GATCGCGCGC 
GCGAGCGGGG GGGAGAAGAA GGAGAAGAAT GCGTACGGTG AGACGGTGCG GTTGCCGAAG 
ACGAGTTTTG AGATGCGGGC GAACAGCGTG GTGAAGGAGC CGAAGATGCA GGAGTGGTGG 
CGGGAGCGCG GGGTGTACGA AACGCTGAGA GATCGAGACG GTGAGGCGTT CGTGCTGCAC 
GATGGACCGC CGTACGCGAA CGGGGATCTA CACATTGGAC ACGCGCTGAA TAAGATTTTG 
AAGGATTTCG TGAACCGGTG GGAGATGATG AATGGGAAAA AGGTGCGCTT CGTCCCTGGG 
TGGGACTGTC ACGGACTCCC GATCGAGCTC AAGGTGTTGC AGAGCATGGA CGCGGAGGCG 
CGAAAGAATT TGACGCCGAT CAAGCTTCGT TACAAGGCGA AGGCGTTCGC GGAGAAGACG 
GTGAAGGCGC AGCGCGAGCA GTTCAAGCGA TACGGCGTGT GGGCCGACTG GGAGGATCCC 
TACATGACGC TCCTGCCAGA GTACGAGGCG GCGCAGATGG AAGTTTTCGG TAGCATGTTC 
CTGAACGGAC ATATTTACCG CGGGAAGAAG CCCGTGCACT GGTCTCCGTC GAGCATGACG 
GCGCTCGCCG AGGCTGAGCT CGAATACCCC GAGGGACACT TCTCGCAGAG CATCTACGTC 
GCGTTTCCGG TCTCCGAAGT TCCAGATTCG ACGCCGAGCG CGCTGAGGGA TCTGCTCAAG 
GACGCATCTC TGGCTATTTG GACAACGACG CCGTGGACGA TGCCGGCGAA CGCGGCGGTG 
GCGGTAAATG CAAAGTTGGA CTACTCCGTG TGCAGAGTGG AATCGACGGG CAAAACGCTC 
GTCGTCGCCG AAGGTTTGCG CGAAGCCGTC GCGGCGAAGT TAGAGTCCGA GCTCACTACT 
CTTGGAACAT TCAAGGGTCA AGATTTCGAG AACGTGCAGT ACTCACATCC GTTCTACGAA 
CGCAAGTCGC CCGTCATCAT CGGCGGCGAG TACATCACCA CCGAAACTGG TACCGGTTTG 
GTTCACACCG CTCCCGGTCA CGGTCAGGAT GATTACATCT CGGGCATGAA GTACGGGTTG 
CCGTTGTATT CGCCGGTTGA TAACGCCGGT TTGTTCACCG CCGAGGCTGG TGGAGATTTG 
GAGGGAAAGG ATGTGTTGGG CGACGGCAAC GTGCTGTGCA TTGAAAAGTT AGAAACAGCT 
GGGGCGCTTC TCAAGCGCGA GAAGTACGGA CACAAATACC CGTACGACTG GCGCACGAAG 
AAGCCTACGA TCTTCCGCGC GACCGAGCAA TGGTTTGCGT CTGTCGAGGG CTTCCGTGAG 
GACGCTCTCA AGGAGTTGGA CAAGTTGCAG TTTATTCCTG AGTCCGGCTC GAAGAGAATG 
CGACCGATGG TTAGTGGGCG CGCTGACTGG TGCATCTCTC GTCAGCGAGC GTGGGGCGTA 
CCGATTCCGG CGTTTTACCA CAAAGACACG AACGAGGTGC TCATGGACGA AGCCATCATC 
AAACACGTCA CAGAGATCGT TCGCGCGAAG GGTACGAACG CGTGGTGGGA GTTGGAGGTC 
GAGGATCTCT TGCCCGAGGA GCACAAGGCC AAGGCGGGTG AGTACGTTCG CGGTACCGAT 
ACTATGGATG TGTGGTTTGA CTCCGGAAGC TCTTGGGCTG GTGTGGTGAA GACCCGCGGT 
ATGTCGTTTC CCGCCGACGT GTACCTGGAG GGCAGCGATC AGCACAGAGG TTGGTTCCAA 
TCGAGTCTTC TCACCGGCGT CGGTGCGATG GGTCAGGCGC CATACAAGAC GATTTTGACG 
CACGGCTTCG TTCTGGATGA GAAAGGATAC AAGATGTCCA AGTCTTTGGG TAACGTTATC 
GACCCTCGCA CGGTGATCGA GGGCGGAAAG AACCAAAAGC AGGAGCCAGG CTACGGCGCC 
GATACGCTTC GCCTGTGGGT TGCGAGCACG GACTATTCCG GAGACGTGAG TATTGGTATG 
AACATCATCA AACAAACGAG CGAGGCGTAT CGCAAGCTCA GAGGGACGAT TCGATTCGCG 
ATGGGCGTTC TGGATGATTT CAATCCGGCG CAGCAAGTGC CGTACGATGA TTTGCCAGTG 
TTCGACAAGT ACGTCCTTCA TCGCTTGAAC GCGACGGTGA AAGAGATTGA GAGCTCCTAC 
AAGGCGTTCG ACTACAGCCG AGTGGTCTCG GCGGTGACCT CTTTCACGAC ATTCCTTTCC 
AACGTCTACT TGGACGTCAG CAAGGATAAG TTGTACATTG GCGCACAAGA TGACTTCAAG 
CGACGAGCAT GTCAGACCGT AGTGTCTGCC TTGGTCGAGC GTATTATCGC TGCAATCGCA 
CCGCTTACGC CGCATATGGC AGAGGAGGCG TTCCAAGCGC TGCCGTACGA TAAGCCCAAC 
GGAGCAATTT CCGTTTTCAT CGCCGGTTGG CCGTCCACCC CGGCGGCCTG GTCCGAGCTC 
GACGCCAAGG ACGTGGAGTT CTGGGACTCA TTCTTAGAGA TCCGCGACGG CGTGAACAAG 
GTGCTCGAAG AAGCTCGTAC TGCCAAGCTT CTCGGAGCGT CTCTCGAGTC GAAGGTTACT 
ATGCACTGCT CTGACGCCAA ATTCCTGGCT AAGCTCACTG GCGAAGGCAT TGCCGAGGAG 
TTGAGATACC TCTTCATCGT TAGCGAAGTC CAGCTCGCTG ATTCTAGCGA TGTCGCAATC 
GCCGGGTGTG ACTTCAAGTC CGTCGTTGAC GTTCCCGGCG CCGGTACCGT GTCCATCGGT 
GTCGCTCGTG CCGCTGGCTC CAAGTGCGCT CGATGCTGGA ACTTCTCCCC TCTCGTCGGC 
TCCGACGCCA AGCACTCGCA GCTGTGCGAG CGGTGCGTCC CGATCGTGAA CGCCACGCAC 
CCGGATCTCG TCGTCCCTGA GCCCGCCGCC GCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OT_ostta04g00400 ( XM_003078517.2 ) cDNA ORF clone, Ostreococcus tauri -> XP_003078565.2 Ostreococcus tauri Isoleucine-tRNA ligase, type 1 (OT_ostta04g00400), partial mRNA.

Price & Availability↑

CloneID	OOd74722
Clone ID Related Accession (Same CDS sequence)	XM_003078517.2
Accession Version	XM_003078517.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1509984000000
Organism	Ostreococcus tauri
Product	Isoleucine-tRNA ligase, type 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_014429). On Nov 8, 2017 this sequence version replaced XM_003078517.1. COMPLETENESS: incomplete on both ends.

ATGGACGACG CGTCGGTCCG GGCGACGCCG ACGGGGGATG ACGTCGACGG CGCGCGCGTC 
GGCGCCGCCC GCATCGCCGC GCGCATGCTC CGCGGCGCGC TCGCGATGAC GACGCGCGCG 
AGGGCGGGGC CGGGGATGTC GTCCACGCGC GCGCGCGCGC GCGCGGTGAT CGCACGACGG 
GCGCGAGGCG GGACGACGCT CGGTCGAACG GCGAGCGCGA GCGCGACGCG ACGCGGGCGA 
GGGACGATCG CGCGCGCGAG CGGGGGGGAG AAGAAGGAGA AGAATGCGTA CGGTGAGACG 
GTGCGGTTGC CGAAGACGAG TTTTGAGATG CGGGCGAACA GCGTGGTGAA GGAGCCGAAG 
ATGCAGGAGT GGTGGCGGGA GCGCGGGGTG TACGAAACGC TGAGAGATCG AGACGGTGAG 
GCGTTCGTGC TGCACGATGG ACCGCCGTAC GCGAACGGGG ATCTACACAT TGGACACGCG 
CTGAATAAGA TTTTGAAGGA TTTCGTGAAC CGGTGGGAGA TGATGAATGG GAAAAAGGTG 
CGCTTCGTCC CTGGGTGGGA CTGTCACGGA CTCCCGATCG AGCTCAAGGT GTTGCAGAGC 
ATGGACGCGG AGGCGCGAAA GAATTTGACG CCGATCAAGC TTCGTTACAA GGCGAAGGCG 
TTCGCGGAGA AGACGGTGAA GGCGCAGCGC GAGCAGTTCA AGCGATACGG CGTGTGGGCC 
GACTGGGAGG ATCCCTACAT GACGCTCCTG CCAGAGTACG AGGCGGCGCA GATGGAAGTT 
TTCGGTAGCA TGTTCCTGAA CGGACATATT TACCGCGGGA AGAAGCCCGT GCACTGGTCT 
CCGTCGAGCA TGACGGCGCT CGCCGAGGCT GAGCTCGAAT ACCCCGAGGG ACACTTCTCG 
CAGAGCATCT ACGTCGCGTT TCCGGTCTCC GAAGTTCCAG ATTCGACGCC GAGCGCGCTG 
AGGGATCTGC TCAAGGACGC ATCTCTGGCT ATTTGGACAA CGACGCCGTG GACGATGCCG 
GCGAACGCGG CGGTGGCGGT AAATGCAAAG TTGGACTACT CCGTGTGCAG AGTGGAATCG 
ACGGGCAAAA CGCTCGTCGT CGCCGAAGGT TTGCGCGAAG CCGTCGCGGC GAAGTTAGAG 
TCCGAGCTCA CTACTCTTGG AACATTCAAG GGTCAAGATT TCGAGAACGT GCAGTACTCA 
CATCCGTTCT ACGAACGCAA GTCGCCCGTC ATCATCGGCG GCGAGTACAT CACCACCGAA 
ACTGGTACCG GTTTGGTTCA CACCGCTCCC GGTCACGGTC AGGATGATTA CATCTCGGGC 
ATGAAGTACG GGTTGCCGTT GTATTCGCCG GTTGATAACG CCGGTTTGTT CACCGCCGAG 
GCTGGTGGAG ATTTGGAGGG AAAGGATGTG TTGGGCGACG GCAACGTGCT GTGCATTGAA 
AAGTTAGAAA CAGCTGGGGC GCTTCTCAAG CGCGAGAAGT ACGGACACAA ATACCCGTAC 
GACTGGCGCA CGAAGAAGCC TACGATCTTC CGCGCGACCG AGCAATGGTT TGCGTCTGTC 
GAGGGCTTCC GTGAGGACGC TCTCAAGGAG TTGGACAAGT TGCAGTTTAT TCCTGAGTCC 
GGCTCGAAGA GAATGCGACC GATGGTTAGT GGGCGCGCTG ACTGGTGCAT CTCTCGTCAG 
CGAGCGTGGG GCGTACCGAT TCCGGCGTTT TACCACAAAG ACACGAACGA GGTGCTCATG 
GACGAAGCCA TCATCAAACA CGTCACAGAG ATCGTTCGCG CGAAGGGTAC GAACGCGTGG 
TGGGAGTTGG AGGTCGAGGA TCTCTTGCCC GAGGAGCACA AGGCCAAGGC GGGTGAGTAC 
GTTCGCGGTA CCGATACTAT GGATGTGTGG TTTGACTCCG GAAGCTCTTG GGCTGGTGTG 
GTGAAGACCC GCGGTATGTC GTTTCCCGCC GACGTGTACC TGGAGGGCAG CGATCAGCAC 
AGAGGTTGGT TCCAATCGAG TCTTCTCACC GGCGTCGGTG CGATGGGTCA GGCGCCATAC 
AAGACGATTT TGACGCACGG CTTCGTTCTG GATGAGAAAG GATACAAGAT GTCCAAGTCT 
TTGGGTAACG TTATCGACCC TCGCACGGTG ATCGAGGGCG GAAAGAACCA AAAGCAGGAG 
CCAGGCTACG GCGCCGATAC GCTTCGCCTG TGGGTTGCGA GCACGGACTA TTCCGGAGAC 
GTGAGTATTG GTATGAACAT CATCAAACAA ACGAGCGAGG CGTATCGCAA GCTCAGAGGG 
ACGATTCGAT TCGCGATGGG CGTTCTGGAT GATTTCAATC CGGCGCAGCA AGTGCCGTAC 
GATGATTTGC CAGTGTTCGA CAAGTACGTC CTTCATCGCT TGAACGCGAC GGTGAAAGAG 
ATTGAGAGCT CCTACAAGGC GTTCGACTAC AGCCGAGTGG TCTCGGCGGT GACCTCTTTC 
ACGACATTCC TTTCCAACGT CTACTTGGAC GTCAGCAAGG ATAAGTTGTA CATTGGCGCA 
CAAGATGACT TCAAGCGACG AGCATGTCAG ACCGTAGTGT CTGCCTTGGT CGAGCGTATT 
ATCGCTGCAA TCGCACCGCT TACGCCGCAT ATGGCAGAGG AGGCGTTCCA AGCGCTGCCG 
TACGATAAGC CCAACGGAGC AATTTCCGTT TTCATCGCCG GTTGGCCGTC CACCCCGGCG 
GCCTGGTCCG AGCTCGACGC CAAGGACGTG GAGTTCTGGG ACTCATTCTT AGAGATCCGC 
GACGGCGTGA ACAAGGTGCT CGAAGAAGCT CGTACTGCCA AGCTTCTCGG AGCGTCTCTC 
GAGTCGAAGG TTACTATGCA CTGCTCTGAC GCCAAATTCC TGGCTAAGCT CACTGGCGAA 
GGCATTGCCG AGGAGTTGAG ATACCTCTTC ATCGTTAGCG AAGTCCAGCT CGCTGATTCT 
AGCGATGTCG CAATCGCCGG GTGTGACTTC AAGTCCGTCG TTGACGTTCC CGGCGCCGGT 
ACCGTGTCCA TCGGTGTCGC TCGTGCCGCT GGCTCCAAGT GCGCTCGATG CTGGAACTTC 
TCCCCTCTCG TCGGCTCCGA CGCCAAGCAC TCGCAGCTGT GCGAGCGGTG CGTCCCGATC 
GTGAACGCCA CGCACCCGGA TCTCGTCGTC CCTGAGCCCG CCGCCGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003078565.2
CDS	1..3171
Translation	MDDASVRATPTGDDVDGARVGAARIAARMLRGALAMTTRARAGPGMSSTRARARAVIARRARGGTTLGRTASASATRRGRGTIARASGGEKKEKNAYGETVRLPKTSFEMRANSVVKEPKMQEWWRERGVYETLRDRDGEAFVLHDGPPYANGDLHIGHALNKILKDFVNRWEMMNGKKVRFVPGWDCHGLPIELKVLQSMDAEARKNLTPIKLRYKAKAFAEKTVKAQREQFKRYGVWADWEDPYMTLLPEYEAAQMEVFGSMFLNGHIYRGKKPVHWSPSSMTALAEAELEYPEGHFSQSIYVAFPVSEVPDSTPSALRDLLKDASLAIWTTTPWTMPANAAVAVNAKLDYSVCRVESTGKTLVVAEGLREAVAAKLESELTTLGTFKGQDFENVQYSHPFYERKSPVIIGGEYITTETGTGLVHTAPGHGQDDYISGMKYGLPLYSPVDNAGLFTAEAGGDLEGKDVLGDGNVLCIEKLETAGALLKREKYGHKYPYDWRTKKPTIFRATEQWFASVEGFREDALKELDKLQFIPESGSKRMRPMVSGRADWCISRQRAWGVPIPAFYHKDTNEVLMDEAIIKHVTEIVRAKGTNAWWELEVEDLLPEEHKAKAGEYVRGTDTMDVWFDSGSSWAGVVKTRGMSFPADVYLEGSDQHRGWFQSSLLTGVGAMGQAPYKTILTHGFVLDEKGYKMSKSLGNVIDPRTVIEGGKNQKQEPGYGADTLRLWVASTDYSGDVSIGMNIIKQTSEAYRKLRGTIRFAMGVLDDFNPAQQVPYDDLPVFDKYVLHRLNATVKEIESSYKAFDYSRVVSAVTSFTTFLSNVYLDVSKDKLYIGAQDDFKRRACQTVVSALVERIIAAIAPLTPHMAEEAFQALPYDKPNGAISVFIAGWPSTPAAWSELDAKDVEFWDSFLEIRDGVNKVLEEARTAKLLGASLESKVTMHCSDAKFLAKLTGEGIAEELRYLFIVSEVQLADSSDVAIAGCDFKSVVDVPGAGTVSIGVARAAGSKCARCWNFSPLVGSDAKHSQLCERCVPIVNATHPDLVVPEPAAA

Target ORF information:

RefSeq Version	XM_003078517.2
Organism	Ostreococcus tauri
Definition	Ostreococcus tauri Isoleucine-tRNA ligase, type 1 (OT_ostta04g00400), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003078517.2

ORF Insert Sequence:

ATGGACGACG CGTCGGTCCG GGCGACGCCG ACGGGGGATG ACGTCGACGG CGCGCGCGTC 
GGCGCCGCCC GCATCGCCGC GCGCATGCTC CGCGGCGCGC TCGCGATGAC GACGCGCGCG 
AGGGCGGGGC CGGGGATGTC GTCCACGCGC GCGCGCGCGC GCGCGGTGAT CGCACGACGG 
GCGCGAGGCG GGACGACGCT CGGTCGAACG GCGAGCGCGA GCGCGACGCG ACGCGGGCGA 
GGGACGATCG CGCGCGCGAG CGGGGGGGAG AAGAAGGAGA AGAATGCGTA CGGTGAGACG 
GTGCGGTTGC CGAAGACGAG TTTTGAGATG CGGGCGAACA GCGTGGTGAA GGAGCCGAAG 
ATGCAGGAGT GGTGGCGGGA GCGCGGGGTG TACGAAACGC TGAGAGATCG AGACGGTGAG 
GCGTTCGTGC TGCACGATGG ACCGCCGTAC GCGAACGGGG ATCTACACAT TGGACACGCG 
CTGAATAAGA TTTTGAAGGA TTTCGTGAAC CGGTGGGAGA TGATGAATGG GAAAAAGGTG 
CGCTTCGTCC CTGGGTGGGA CTGTCACGGA CTCCCGATCG AGCTCAAGGT GTTGCAGAGC 
ATGGACGCGG AGGCGCGAAA GAATTTGACG CCGATCAAGC TTCGTTACAA GGCGAAGGCG 
TTCGCGGAGA AGACGGTGAA GGCGCAGCGC GAGCAGTTCA AGCGATACGG CGTGTGGGCC 
GACTGGGAGG ATCCCTACAT GACGCTCCTG CCAGAGTACG AGGCGGCGCA GATGGAAGTT 
TTCGGTAGCA TGTTCCTGAA CGGACATATT TACCGCGGGA AGAAGCCCGT GCACTGGTCT 
CCGTCGAGCA TGACGGCGCT CGCCGAGGCT GAGCTCGAAT ACCCCGAGGG ACACTTCTCG 
CAGAGCATCT ACGTCGCGTT TCCGGTCTCC GAAGTTCCAG ATTCGACGCC GAGCGCGCTG 
AGGGATCTGC TCAAGGACGC ATCTCTGGCT ATTTGGACAA CGACGCCGTG GACGATGCCG 
GCGAACGCGG CGGTGGCGGT AAATGCAAAG TTGGACTACT CCGTGTGCAG AGTGGAATCG 
ACGGGCAAAA CGCTCGTCGT CGCCGAAGGT TTGCGCGAAG CCGTCGCGGC GAAGTTAGAG 
TCCGAGCTCA CTACTCTTGG AACATTCAAG GGTCAAGATT TCGAGAACGT GCAGTACTCA 
CATCCGTTCT ACGAACGCAA GTCGCCCGTC ATCATCGGCG GCGAGTACAT CACCACCGAA 
ACTGGTACCG GTTTGGTTCA CACCGCTCCC GGTCACGGTC AGGATGATTA CATCTCGGGC 
ATGAAGTACG GGTTGCCGTT GTATTCGCCG GTTGATAACG CCGGTTTGTT CACCGCCGAG 
GCTGGTGGAG ATTTGGAGGG AAAGGATGTG TTGGGCGACG GCAACGTGCT GTGCATTGAA 
AAGTTAGAAA CAGCTGGGGC GCTTCTCAAG CGCGAGAAGT ACGGACACAA ATACCCGTAC 
GACTGGCGCA CGAAGAAGCC TACGATCTTC CGCGCGACCG AGCAATGGTT TGCGTCTGTC 
GAGGGCTTCC GTGAGGACGC TCTCAAGGAG TTGGACAAGT TGCAGTTTAT TCCTGAGTCC 
GGCTCGAAGA GAATGCGACC GATGGTTAGT GGGCGCGCTG ACTGGTGCAT CTCTCGTCAG 
CGAGCGTGGG GCGTACCGAT TCCGGCGTTT TACCACAAAG ACACGAACGA GGTGCTCATG 
GACGAAGCCA TCATCAAACA CGTCACAGAG ATCGTTCGCG CGAAGGGTAC GAACGCGTGG 
TGGGAGTTGG AGGTCGAGGA TCTCTTGCCC GAGGAGCACA AGGCCAAGGC GGGTGAGTAC 
GTTCGCGGTA CCGATACTAT GGATGTGTGG TTTGACTCCG GAAGCTCTTG GGCTGGTGTG 
GTGAAGACCC GCGGTATGTC GTTTCCCGCC GACGTGTACC TGGAGGGCAG CGATCAGCAC 
AGAGGTTGGT TCCAATCGAG TCTTCTCACC GGCGTCGGTG CGATGGGTCA GGCGCCATAC 
AAGACGATTT TGACGCACGG CTTCGTTCTG GATGAGAAAG GATACAAGAT GTCCAAGTCT 
TTGGGTAACG TTATCGACCC TCGCACGGTG ATCGAGGGCG GAAAGAACCA AAAGCAGGAG 
CCAGGCTACG GCGCCGATAC GCTTCGCCTG TGGGTTGCGA GCACGGACTA TTCCGGAGAC 
GTGAGTATTG GTATGAACAT CATCAAACAA ACGAGCGAGG CGTATCGCAA GCTCAGAGGG 
ACGATTCGAT TCGCGATGGG CGTTCTGGAT GATTTCAATC CGGCGCAGCA AGTGCCGTAC 
GATGATTTGC CAGTGTTCGA CAAGTACGTC CTTCATCGCT TGAACGCGAC GGTGAAAGAG 
ATTGAGAGCT CCTACAAGGC GTTCGACTAC AGCCGAGTGG TCTCGGCGGT GACCTCTTTC 
ACGACATTCC TTTCCAACGT CTACTTGGAC GTCAGCAAGG ATAAGTTGTA CATTGGCGCA 
CAAGATGACT TCAAGCGACG AGCATGTCAG ACCGTAGTGT CTGCCTTGGT CGAGCGTATT 
ATCGCTGCAA TCGCACCGCT TACGCCGCAT ATGGCAGAGG AGGCGTTCCA AGCGCTGCCG 
TACGATAAGC CCAACGGAGC AATTTCCGTT TTCATCGCCG GTTGGCCGTC CACCCCGGCG 
GCCTGGTCCG AGCTCGACGC CAAGGACGTG GAGTTCTGGG ACTCATTCTT AGAGATCCGC 
GACGGCGTGA ACAAGGTGCT CGAAGAAGCT CGTACTGCCA AGCTTCTCGG AGCGTCTCTC 
GAGTCGAAGG TTACTATGCA CTGCTCTGAC GCCAAATTCC TGGCTAAGCT CACTGGCGAA 
GGCATTGCCG AGGAGTTGAG ATACCTCTTC ATCGTTAGCG AAGTCCAGCT CGCTGATTCT 
AGCGATGTCG CAATCGCCGG GTGTGACTTC AAGTCCGTCG TTGACGTTCC CGGCGCCGGT 
ACCGTGTCCA TCGGTGTCGC TCGTGCCGCT GGCTCCAAGT GCGCTCGATG CTGGAACTTC 
TCCCCTCTCG TCGGCTCCGA CGCCAAGCAC TCGCAGCTGT GCGAGCGGTG CGTCCCGATC 
GTGAACGCCA CGCACCCGGA TCTCGTCGTC CCTGAGCCCG CCGCCGCGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome of the model marine alga Ostreococcus tauri unfolds by assessing Illumina de novo assemblies.
BMC genomics151103(2014 Dec)
Blanc-Mathieu R,Verhelst B,Derelle E,Rombauts S,Bouget FY,Carré I,Château A,Eyre-Walker A,Grimsley N,Moreau H,Piégu B,Rivals E,Schackwitz W,Van de Peer Y,Piganeau G

Genome analysis of the smallest free-living eukaryote Ostreococcus tauri unveils many unique features.
Proceedings of the National Academy of Sciences of the United States of America103(31)11647-52(2006 Aug)
Derelle E,Ferraz C,Rombauts S,Rouzé P,Worden AZ,Robbens S,Partensky F,Degroeve S,Echeynié S,Cooke R,Saeys Y,Wuyts J,Jabbari K,Bowler C,Panaud O,Piégu B,Ball SG,Ral JP,Bouget FY,Piganeau G,De Baets B,Picard A,Delseny M,Demaille J,Van de Peer Y,Moreau H

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OT_ostta04g00400 cDNA ORF clone, Ostreococcus tauri

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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