LOC101174706 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101174706
Entrez Gene ID	101174706
Full Name	collagen alpha-1(III) chain
Synonyms	fus
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020712200.1	XP_020567859.1	fibroin heavy chain isoform X2
XM_020712203.1	XP_020567862.1	fibroin heavy chain isoform X6
XM_020712202.1	XP_020567861.1	fibroin heavy chain isoform X5
XM_020712199.1	XP_020567858.1	fibroin heavy chain isoform X1
XM_020712201.1	XP_020567860.1	fibroin heavy chain isoform X3
XM_011488030.2	XP_011486332.1	fibroin heavy chain isoform X4
XM_011488035.2	XP_011486337.1	collagen alpha-1(III) chain isoform X7

Pathways from BioSystems
Gene Ontology

The following LOC101174706 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101174706 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa36527	XM_011488030.2 Latest version!	Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OJa36532	XM_011488035.2 Latest version!	Oryzias latipes collagen alpha-1(III) chain (LOC101174706), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OJa74382	XM_020712199.1 Latest version!	Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OJa74383	XM_020712200.1 Latest version!	Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OJa74384	XM_020712201.1 Latest version!	Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OJa74385	XM_020712202.1 Latest version!	Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OJa74386	XM_020712203.1 Latest version!	Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101174706 ( XM_011488030.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011486332.1 Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OJa36527
Clone ID Related Accession (Same CDS sequence)	XM_011488030.2
Accession Version	XM_011488030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	fibroin heavy chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019877.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011488030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGAC GATGCTTCCA TCTCACTACG GTGATCCTCT GGTTGGTGCA GGCAGTGCAT 
TCGGGGGGAT ATAAACCACA GAATGGCAGA GGTGTGGGGA CATTGCTGAT GCCATCAAAA 
GGTGTGGGTC ATGCAGTGAG GCCACAAAAC GGATATGCTG GATATCCCAT GAAGGGACCA 
GGTTATGGAG CCACAGCTGG GACGAACAAA GGCAGTGGAA TGAAAGGTTA CGGACAAACG 
GCTGGACAGA TGAGTGAACA AGGGGGTAGA CAGCTGGGAT ATGGTGTCAA AGCCGGCCCA 
GTCACTGGAC AGATGAACGG AGGCTTTGGT GCTCAACAAG CATTTGGTGG AGCAGGAGCC 
AAAGGCAATG GAAGAAATCC TGCAGGTTCG TTCAACAGAA ATGGAGTACA ACCTAATGTA 
GGATATGGCG TTTCAGCTGG GCCGTTATCT GGACCACAAA TCAAAGGTTT CAATCCTCAG 
GCTGGTGGAT ATGGAGCGAC GAAAGGAAAT GGTTATGAGG CAGCAGCTGC CTCACAGTAT 
ACACCTGGAA TAAGAGGCAA TGTATATGGA TCTAAAGCTG GCCCAAGCAT CAGACAACTG 
AAATCTGGTT ATGTTGCTCA AACTGGTGGT AATAGTGGGC AAGGAGAAAA ACCCAATGGG 
TATGGTGCTG TGGCTGGCCC AAGCACAGGA CAACAAATGA GAAATGGTTA CAATCTTCAA 
TCTGGAGGAC AAACGACTAA AGGCAATGGT CAAGGCGTGG TGGCTGGACC CTCTACGGCA 
AATGGAGCAT ATGGACAAGG AGGAATGGGA AGCAAACTTA TGAAGGGTTA TGGCCGAACA 
CCTTATGGAT CAGGTGTTCA GAGGGGAGTG TTGGGAGGCC TCGGAGTTCC TCGGCCTGCT 
AGGAACCAGC AGAAATCGTA TGGGAGCAGA ACTGGTGACA GAGGTCAGCC CAACAGCGGC 
TACAATGGCG GTTATGGCAG CACAGCTCTG GGACCTCGTT ATGGAAATGG CGGGATGAAA 
GGACCGGTAA AAGGCTATGC AAACTTACCA CATACTGGTA ATAGAGGAGC AGTTCTAAAC 
GGTTATGAAC AACAAGGCGG TGGAGTAGCT GCAAAACCAG CAAAATCAGG TGGTGGTAGT 
ATTCCTAATG GATATGGTGC CAGACCTAAC GGAAATCCTC ATTTAAATGG AGGTTCAGGG 
AGACCCCAGC CAAGTTTTGG AGCCACAGCT GAAGGCGGAG TTTTCAAAGG ATATGGCACT 
AAACCAAGTG GTCATGGTGG AAATGGTGCT GTTCTGAGTG GATATGGACG ACAGCCCAAC 
GGTTACAACC TGATGCCAAA TGGAAAGGGC CAGGCTGTTA GAGGAACAGA GGTATCAGGT 
GAAAGGAACC TCAAAGGTGG TGTGCACTTC CACCACCATC ATCATCAGCA GCAGCAGATG 
ACGGCACAGT TGGGGGATGT CACCCCACGA GCAGCTACCC CACCTATCCC GGCCCCTACA 
GGTGGTCTCC TTGTTATGGT GACCCAAGAG AAGTACCAAA AGCTGCCTTC ACCTTTGCCC 
CAGGGGAAAA AGTACAAACA CACAGCAATC CTGCCTGAGG AAAGCCCAGA ACCAGCACCT 
GCATATCCTG AAGCCAGAGG GCAGCACCTC TCACTGGAGC CTGCACCTAT TGGTCCAAAG 
GACAAGAATC AGAAGGGGGT CCATTCTGGA CCTGCAGTCG TGGTTCCCCA AAGCAAACCC 
GCACCAGAAC CAGCAGCTGT TCAAGGTTAT GTGACTGGAA ATGCTGTTCA TCTGCAAAAA 
GCTGCAGAAG CAGACGCTGT AGGTTTGGGG GGGGGCGCCC CAAAAACCAA AGCTGTTCCT 
GAAACAGCTG AGGAAAGGGG CGGTGTCCCT CCAAAGGAAA CACTGATCAC TGCGGGTGTG 
GGCGAGTCTG CAGCAGAGGA GGCTGACGCA CAACCTGACA GGCTGCAGGG CACAAGTATC 
CATGCTGGGG AGACGGAGAG TAGGGGATCT TCTGCCTCAA AGGGACCAGG AGCCAAACTT 
GACTGTGGAC TGAATGGTCA GTGGATGAAA ATACCAAGAC CTGATTACAA TGCAGCAGCT 
GGTAGTTTGG ATGGCTCACA AACTAAAGGT TATGGAGCAG GAGAAAATGC TTTTCTAAAC 
AATGGATATG GAGCAGGGCT TGGGTACCCG TATGCAGGGA AAGCAGCTCA AGCTGGATAT 
GGAAAAGGAG TTCACCCTGG GGCTGGATAT GAAAATGGAA ATCCATATGG AGCTCTCAGT 
GAAGCAGGCT ATAGAAATGG GTATGGAGCA GGACCTCAGC CTGACTATTC AAGTTATGGT 
CAAGGTGTGC CGACTATTGA TGGCAAATCA GGTGGAAACC CAGGACTTCC TGCAGGACTA 
GATGGAATGA GTCAGTTTGA GCCTCAGTCT GTTGGTCTGG GTCCAAATGG AAAAGGCGTC 
ATGTATGGTG TTGGGGGGCT GCAGTTTGGT GGACAACCTG GGAACTCCGG GATAAATGGT 
GCAGGACAGT ATGGAGGTGT CGTTGCAAGT ATGGGAGGGA GTCTTGTGCC TGGAATGCAC 
AGTTATGGTG GATATGCTTA TAACCAAAAT GCTCCTGGAA AACAGGGTTA TGGAAGACTT 
CCTTATGGAG TTCAACAGGC TGGACTTAAC CCTGAGGCTA AAGCCGCCGC CCAGTATGGT 
CATACTGGGT CATCATATCA GCCTGAATCT GTTGGCTTCG GACAACCAGG GAAAACAGCA 
GGATCATACA GCGGGGGACA GGCTGCCTTT GCACCTCAGG CTCTTGGGTT TGGGGGTGAT 
GATAAATCTG GTAAATATGA TAAACAGGGA GCTTTCCAAT CACAGCGTCT CCGGCCTGAA 
GAAACAGCCG GTCAGACATA TGAGCGCCAG TCTGTTGGGT TGGATTCAGC AGGAAAGTCC 
TATGTGAAAG GGGAAGTGCC AACTGCAACT CTAGCAGCTG AAAGTGAGAG GATTCCCATT 
AATAAATATG AAAATGTGGG CTACATAAAT GGACAAATGC AGCCAGAAGT TGCTGGGCTT 
CCTGCAGCTC CCACTGCCAG TTCCACCCTG GCCTTTTCCT CTCCTGATGG GATTCCTGTA 
GAGGATGTTC AGGACATTCC TGATCCAGAG GGCACAGCCA GCTTCTTGGA GTCGGGACCC 
GTTACAGAAA TGCTGGGCGA GTCTCGGGGA CCGGAGGATG CCGAGAACCT GCCACGTCAG 
ATACACATCC AGCAGCATCT CAAACTCCAT TTCAACCCAC AAGGCAAAAA AAATGGCAAA 
TATGATGCAA ATGGCTTCTT TGGGAACAAT GGCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011486332.1
CDS	113..3451
Translation	MVRRCFHLTTVILWLVQAVHSGGYKPQNGRGVGTLLMPSKGVGHAVRPQNGYAGYPMKGPGYGATAGTNKGSGMKGYGQTAGQMSEQGGRQLGYGVKAGPVTGQMNGGFGAQQAFGGAGAKGNGRNPAGSFNRNGVQPNVGYGVSAGPLSGPQIKGFNPQAGGYGATKGNGYEAAAASQYTPGIRGNVYGSKAGPSIRQLKSGYVAQTGGNSGQGEKPNGYGAVAGPSTGQQMRNGYNLQSGGQTTKGNGQGVVAGPSTANGAYGQGGMGSKLMKGYGRTPYGSGVQRGVLGGLGVPRPARNQQKSYGSRTGDRGQPNSGYNGGYGSTALGPRYGNGGMKGPVKGYANLPHTGNRGAVLNGYEQQGGGVAAKPAKSGGGSIPNGYGARPNGNPHLNGGSGRPQPSFGATAEGGVFKGYGTKPSGHGGNGAVLSGYGRQPNGYNLMPNGKGQAVRGTEVSGERNLKGGVHFHHHHHQQQQMTAQLGDVTPRAATPPIPAPTGGLLVMVTQEKYQKLPSPLPQGKKYKHTAILPEESPEPAPAYPEARGQHLSLEPAPIGPKDKNQKGVHSGPAVVVPQSKPAPEPAAVQGYVTGNAVHLQKAAEADAVGLGGGAPKTKAVPETAEERGGVPPKETLITAGVGESAAEEADAQPDRLQGTSIHAGETESRGSSASKGPGAKLDCGLNGQWMKIPRPDYNAAAGSLDGSQTKGYGAGENAFLNNGYGAGLGYPYAGKAAQAGYGKGVHPGAGYENGNPYGALSEAGYRNGYGAGPQPDYSSYGQGVPTIDGKSGGNPGLPAGLDGMSQFEPQSVGLGPNGKGVMYGVGGLQFGGQPGNSGINGAGQYGGVVASMGGSLVPGMHSYGGYAYNQNAPGKQGYGRLPYGVQQAGLNPEAKAAAQYGHTGSSYQPESVGFGQPGKTAGSYSGGQAAFAPQALGFGGDDKSGKYDKQGAFQSQRLRPEETAGQTYERQSVGLDSAGKSYVKGEVPTATLAAESERIPINKYENVGYINGQMQPEVAGLPAAPTASSTLAFSSPDGIPVEDVQDIPDPEGTASFLESGPVTEMLGESRGPEDAENLPRQIHIQQHLKLHFNPQGKKNGKYDANGFFGNNGY

Target ORF information:

RefSeq Version	XM_011488030.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011488030.2

ORF Insert Sequence:

ATGGTGAGAC GATGCTTCCA TCTCACTACG GTGATCCTCT GGTTGGTGCA GGCAGTGCAT 
TCGGGGGGAT ATAAACCACA GAATGGCAGA GGTGTGGGGA CATTGCTGAT GCCATCAAAA 
GGTGTGGGTC ATGCAGTGAG GCCACAAAAC GGATATGCTG GATATCCCAT GAAGGGACCA 
GGTTATGGAG CCACAGCTGG GACGAACAAA GGCAGTGGAA TGAAAGGTTA CGGACAAACG 
GCTGGACAGA TGAGTGAACA AGGGGGTAGA CAGCTGGGAT ATGGTGTCAA AGCCGGCCCA 
GTCACTGGAC AGATGAACGG AGGCTTTGGT GCTCAACAAG CATTTGGTGG AGCAGGAGCC 
AAAGGCAATG GAAGAAATCC TGCAGGTTCG TTCAACAGAA ATGGAGTACA ACCTAATGTA 
GGATATGGCG TTTCAGCTGG GCCGTTATCT GGACCACAAA TCAAAGGTTT CAATCCTCAG 
GCTGGTGGAT ATGGAGCGAC GAAAGGAAAT GGTTATGAGG CAGCAGCTGC CTCACAGTAT 
ACACCTGGAA TAAGAGGCAA TGTATATGGA TCTAAAGCTG GCCCAAGCAT CAGACAACTG 
AAATCTGGTT ATGTTGCTCA AACTGGTGGT AATAGTGGGC AAGGAGAAAA ACCCAATGGG 
TATGGTGCTG TGGCTGGCCC AAGCACAGGA CAACAAATGA GAAATGGTTA CAATCTTCAA 
TCTGGAGGAC AAACGACTAA AGGCAATGGT CAAGGCGTGG TGGCTGGACC CTCTACGGCA 
AATGGAGCAT ATGGACAAGG AGGAATGGGA AGCAAACTTA TGAAGGGTTA TGGCCGAACA 
CCTTATGGAT CAGGTGTTCA GAGGGGAGTG TTGGGAGGCC TCGGAGTTCC TCGGCCTGCT 
AGGAACCAGC AGAAATCGTA TGGGAGCAGA ACTGGTGACA GAGGTCAGCC CAACAGCGGC 
TACAATGGCG GTTATGGCAG CACAGCTCTG GGACCTCGTT ATGGAAATGG CGGGATGAAA 
GGACCGGTAA AAGGCTATGC AAACTTACCA CATACTGGTA ATAGAGGAGC AGTTCTAAAC 
GGTTATGAAC AACAAGGCGG TGGAGTAGCT GCAAAACCAG CAAAATCAGG TGGTGGTAGT 
ATTCCTAATG GATATGGTGC CAGACCTAAC GGAAATCCTC ATTTAAATGG AGGTTCAGGG 
AGACCCCAGC CAAGTTTTGG AGCCACAGCT GAAGGCGGAG TTTTCAAAGG ATATGGCACT 
AAACCAAGTG GTCATGGTGG AAATGGTGCT GTTCTGAGTG GATATGGACG ACAGCCCAAC 
GGTTACAACC TGATGCCAAA TGGAAAGGGC CAGGCTGTTA GAGGAACAGA GGTATCAGGT 
GAAAGGAACC TCAAAGGTGG TGTGCACTTC CACCACCATC ATCATCAGCA GCAGCAGATG 
ACGGCACAGT TGGGGGATGT CACCCCACGA GCAGCTACCC CACCTATCCC GGCCCCTACA 
GGTGGTCTCC TTGTTATGGT GACCCAAGAG AAGTACCAAA AGCTGCCTTC ACCTTTGCCC 
CAGGGGAAAA AGTACAAACA CACAGCAATC CTGCCTGAGG AAAGCCCAGA ACCAGCACCT 
GCATATCCTG AAGCCAGAGG GCAGCACCTC TCACTGGAGC CTGCACCTAT TGGTCCAAAG 
GACAAGAATC AGAAGGGGGT CCATTCTGGA CCTGCAGTCG TGGTTCCCCA AAGCAAACCC 
GCACCAGAAC CAGCAGCTGT TCAAGGTTAT GTGACTGGAA ATGCTGTTCA TCTGCAAAAA 
GCTGCAGAAG CAGACGCTGT AGGTTTGGGG GGGGGCGCCC CAAAAACCAA AGCTGTTCCT 
GAAACAGCTG AGGAAAGGGG CGGTGTCCCT CCAAAGGAAA CACTGATCAC TGCGGGTGTG 
GGCGAGTCTG CAGCAGAGGA GGCTGACGCA CAACCTGACA GGCTGCAGGG CACAAGTATC 
CATGCTGGGG AGACGGAGAG TAGGGGATCT TCTGCCTCAA AGGGACCAGG AGCCAAACTT 
GACTGTGGAC TGAATGGTCA GTGGATGAAA ATACCAAGAC CTGATTACAA TGCAGCAGCT 
GGTAGTTTGG ATGGCTCACA AACTAAAGGT TATGGAGCAG GAGAAAATGC TTTTCTAAAC 
AATGGATATG GAGCAGGGCT TGGGTACCCG TATGCAGGGA AAGCAGCTCA AGCTGGATAT 
GGAAAAGGAG TTCACCCTGG GGCTGGATAT GAAAATGGAA ATCCATATGG AGCTCTCAGT 
GAAGCAGGCT ATAGAAATGG GTATGGAGCA GGACCTCAGC CTGACTATTC AAGTTATGGT 
CAAGGTGTGC CGACTATTGA TGGCAAATCA GGTGGAAACC CAGGACTTCC TGCAGGACTA 
GATGGAATGA GTCAGTTTGA GCCTCAGTCT GTTGGTCTGG GTCCAAATGG AAAAGGCGTC 
ATGTATGGTG TTGGGGGGCT GCAGTTTGGT GGACAACCTG GGAACTCCGG GATAAATGGT 
GCAGGACAGT ATGGAGGTGT CGTTGCAAGT ATGGGAGGGA GTCTTGTGCC TGGAATGCAC 
AGTTATGGTG GATATGCTTA TAACCAAAAT GCTCCTGGAA AACAGGGTTA TGGAAGACTT 
CCTTATGGAG TTCAACAGGC TGGACTTAAC CCTGAGGCTA AAGCCGCCGC CCAGTATGGT 
CATACTGGGT CATCATATCA GCCTGAATCT GTTGGCTTCG GACAACCAGG GAAAACAGCA 
GGATCATACA GCGGGGGACA GGCTGCCTTT GCACCTCAGG CTCTTGGGTT TGGGGGTGAT 
GATAAATCTG GTAAATATGA TAAACAGGGA GCTTTCCAAT CACAGCGTCT CCGGCCTGAA 
GAAACAGCCG GTCAGACATA TGAGCGCCAG TCTGTTGGGT TGGATTCAGC AGGAAAGTCC 
TATGTGAAAG GGGAAGTGCC AACTGCAACT CTAGCAGCTG AAAGTGAGAG GATTCCCATT 
AATAAATATG AAAATGTGGG CTACATAAAT GGACAAATGC AGCCAGAAGT TGCTGGGCTT 
CCTGCAGCTC CCACTGCCAG TTCCACCCTG GCCTTTTCCT CTCCTGATGG GATTCCTGTA 
GAGGATGTTC AGGACATTCC TGATCCAGAG GGCACAGCCA GCTTCTTGGA GTCGGGACCC 
GTTACAGAAA TGCTGGGCGA GTCTCGGGGA CCGGAGGATG CCGAGAACCT GCCACGTCAG 
ATACACATCC AGCAGCATCT CAAACTCCAT TTCAACCCAC AAGGCAAAAA AAATGGCAAA 
TATGATGCAA ATGGCTTCTT TGGGAACAAT GGCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101174706 ( XM_011488035.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011486337.1 Oryzias latipes collagen alpha-1(III) chain (LOC101174706), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OJa36532
Clone ID Related Accession (Same CDS sequence)	XM_011488035.2
Accession Version	XM_011488035.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	collagen alpha-1(III) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011488035.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGAC GATGCTTCCA TCTCACTACG GTGATCCTCT GGTTGGTGCA GGCAGTGCAT 
TCGGGGGGAT ATAAACCACA GAATGGCAGA GGTGTGGGGA CATTGCTGAT GCCATCAAAA 
GGTGTGGGTC ATGCAGTGAG GCCACAAAAC GGATATGCTG GATATCCCAT GAAGGGACCA 
GGTTATGGAG CCACAGCTGG GACGAACAAA GGCAGTGGAA TGAAAGGTTA CGGACAAACG 
GCTGGACAGA TGAGTGAACA AGGGGGTAGA CAGCTGGGAT ATGGTGTCAA AGCCGGCCCA 
GTCACTGGAC AGATGAACGG AGGCTTTGGT GCTCAACAAG CATTTGGTGG AGCAGGAGCC 
AAAGGCAATG GAAGAAATCC TGCAGGTTCG TTCAACAGAA ATGGAGTACA ACCTAATGTA 
GGATATGGCG TTTCAGCTGG GCCGTTATCT GGACCACAAA TCAAAGGTTT CAATCCTCAG 
GCTGGTGGAT ATGGAGCGAC GAAAGGAAAT GGTTATGAGG CAGCAGCTGC CTCACAGTAT 
ACACCTGGAA TAAGAGGCAA TGTATATGGA TCTAAAGCTG GCCCAAGCAT CAGACAACTG 
AAATCTGGTT ATGTTGCTCA AACTGGTGGT AATAGTGGGC AAGGAGAAAA ACCCAATGGG 
TATGGTGCTG TGGCTGGCCC AAGCACAGGA CAACAAATGA GAAATGGTTA CAATCTTCAA 
TCTGGAGGAC AAACGACTAA AGGCAATGGT CAAGGCGTGG TGGCTGGACC CTCTACGGCA 
AATGGAGCAT ATGGACAAGG AGGAATGGGA AGCAAACTTA TGAAGGGTTA TGGCCGAACA 
CCTTATGGAT CAGGTGTTCA GAGGGGAGTG TTGGGAGGCC TCGGAGTTCC TCGGCCTGCT 
AGGAACCAGC AGAAATCGTA TGGGAGCAGA ACTGGTGACA GAGGTCAGCC CAACAGCGGC 
TACAATGGCG GTTATGGCAG CACAGCTCTG GGACCTCGTT ATGGAAATGG CGGGATGAAA 
GGACCGGTAA AAGGCTATGC AAACTTACCA CATACTGGTA ATAGAGGAGC AGTTCTAAAC 
GGTTATGAAC AACAAGGCGG TGGAGTAGCT GCAAAACCAG CAAAATCAGG TGGTGGTAGT 
ATTCCTAATG GATATGGTGC CAGACCTAAC GGAAATCCTC ATTTAAATGG AGGTTCAGGG 
AGACCCCAGC CAAGTTTTGG AGCCACAGCT GAAGGCGGAG TTTTCAAAGG ATATGGCACT 
AAACCAAGTG GTCATGGTGG AAATGGTGCT GTTCTGAGTG GATATGGACG ACAGCCCAAC 
GGTTACAACC TGATGCCAAA TGGAAAGGGC CAGGCTGTTA GAGGAACAGA GGTATCAGGT 
GAAAGGAACC TCAAAGGTGG TGTGCACTTC CACCACCATC ATCATCAGCA GCAGCAGATG 
ACGGCACAGT TGGGGGATGT CACCCCACGA GCAGCTACCC CACCTATCCC GGCCCCTACA 
GGTGGTCTCC TTGTTATGGT GACCCAAGAG AAGTACCAAA AGCTGCCTTC ACCTTTGCCC 
CAGGGGAAAA AGTACAAACA CACAGCAATC CTGCCTGAGG AAAGCCCAGA ACCAGCACCT 
GCATATCCTG AAGCCAGAGG GCAGCACCTC TCACTGGAGC CTGCACCTAT TGGTCCAAAG 
GACAAGAATC AGAAGGGGGT CCATTCTGGA CCTGCAGTCG TGGTTCCCCA AAGCAAACCC 
GCACCAGAAC CAGCAGCTGT TCAAGAGAGT AGGGGATCTT CTGCCTCAAA GGGACCAGGA 
GCCAAACTTG ACTGTGGACT GAATGGTCAG TGGATGAAAA TACCAAGACC TGATTACAAT 
GCAGCAGCTG GTAGTTTGGA TGGCTCACAA ACTAAAGGTT ATGGAGCAGG AGAAAATGCT 
TTTCTAAACA ATGGATATGG AGCAGGGCTT GGGTACCCGT ATGCAGGGAA AGCAGCTCAA 
GCTGGATATG GAAAAGGAGT TCACCCTGGG GCTGGATATG AAAATGGAAA TCCATATGGA 
GCTCTCAGTG AAGCAGGCTA TAGAAATGGG TATGGAGCAG GACCTCAGCC TGACTATTCA 
AGTTATGGTC AAGGTGTGCC GACTATTGAT GGCAAATCAG GTGGAAACCC AGGACTTCCT 
GCAGGACTAG ATGGAATGAG TCAGTTTGAG CCTCAGTCTG TTGGTCTGGG TCCAAATGGA 
AAAGGCGTCA TGTATGGTGT TGGGGGGCTG CAGTTTGGTG GACAACCTGG GAACTCCGGG 
ATAAATGGTG CAGGACAGTA TGGAGGTGTC GTTGCAAGTA TGGGAGGGAG TCTTGTGCCT 
GGAATGCACA GTTATGGTGG ATATGCTTAT AACCAAAATG CTCCTGGAAA ACAGGGTTAT 
GGAAGACTTC CTTATGGAGT TCAACAGGCT GGACTTAACC CTGAGGCTAA AGCCGCCGCC 
CAGTATGGTC ATACTGGGTC ATCATATCAG CCTGAATCTG TTGGCTTCGG ACAACCAGGG 
AAAACAGCAG GATCATACAG CGGGGGACAG GCTGCCTTTG CACCTCAGGC TCTTGGGTTT 
GGGGGTGATG ATAAATCTGG TAAATATGAT AAACAGGGAG CTTTCCAATC ACAGCGTCTC 
CGGCCTGAAG AAACAGCCGG TCAGACATAT GAGCGCCAGT CTGTTGGGTT GGATTCAGCA 
GGAAAGTCCT ATGTGAAAGG GGAAGTGCCA ACTGCAACTC TAGCAGCTGA AAGTGAGAGG 
ATTCCCATTA ATAAATATGA AAATGTGGGC TACATAAATG GACAAATGCA GCCAGAAGTT 
GCTGGGCTTC CTGCAGCTCC CACTGCCAGT TCCACCCTGG CCTTTTCCTC TCCTGATGGG 
ATTCCTGTAG AGGATGTTCA GGACATTCCT GATCCAGAGG GCACAGCCAG CTTCTTGGAG 
TCGGGACCCG TTACAGAAAT GCTGGGCGAG TCTCGGGGAC CGGAGGATGC CGAGAACCTG 
CCACGTCAGA TACACATCCA GCAGCATCTC AAACTCCATT TCAACCCACA AGGCAAAAAA 
AATGGCAAAT ATGATGCAAA TGGCTTCTTT GGGAACAATG GCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011486337.1
CDS	113..3220
Translation	MVRRCFHLTTVILWLVQAVHSGGYKPQNGRGVGTLLMPSKGVGHAVRPQNGYAGYPMKGPGYGATAGTNKGSGMKGYGQTAGQMSEQGGRQLGYGVKAGPVTGQMNGGFGAQQAFGGAGAKGNGRNPAGSFNRNGVQPNVGYGVSAGPLSGPQIKGFNPQAGGYGATKGNGYEAAAASQYTPGIRGNVYGSKAGPSIRQLKSGYVAQTGGNSGQGEKPNGYGAVAGPSTGQQMRNGYNLQSGGQTTKGNGQGVVAGPSTANGAYGQGGMGSKLMKGYGRTPYGSGVQRGVLGGLGVPRPARNQQKSYGSRTGDRGQPNSGYNGGYGSTALGPRYGNGGMKGPVKGYANLPHTGNRGAVLNGYEQQGGGVAAKPAKSGGGSIPNGYGARPNGNPHLNGGSGRPQPSFGATAEGGVFKGYGTKPSGHGGNGAVLSGYGRQPNGYNLMPNGKGQAVRGTEVSGERNLKGGVHFHHHHHQQQQMTAQLGDVTPRAATPPIPAPTGGLLVMVTQEKYQKLPSPLPQGKKYKHTAILPEESPEPAPAYPEARGQHLSLEPAPIGPKDKNQKGVHSGPAVVVPQSKPAPEPAAVQESRGSSASKGPGAKLDCGLNGQWMKIPRPDYNAAAGSLDGSQTKGYGAGENAFLNNGYGAGLGYPYAGKAAQAGYGKGVHPGAGYENGNPYGALSEAGYRNGYGAGPQPDYSSYGQGVPTIDGKSGGNPGLPAGLDGMSQFEPQSVGLGPNGKGVMYGVGGLQFGGQPGNSGINGAGQYGGVVASMGGSLVPGMHSYGGYAYNQNAPGKQGYGRLPYGVQQAGLNPEAKAAAQYGHTGSSYQPESVGFGQPGKTAGSYSGGQAAFAPQALGFGGDDKSGKYDKQGAFQSQRLRPEETAGQTYERQSVGLDSAGKSYVKGEVPTATLAAESERIPINKYENVGYINGQMQPEVAGLPAAPTASSTLAFSSPDGIPVEDVQDIPDPEGTASFLESGPVTEMLGESRGPEDAENLPRQIHIQQHLKLHFNPQGKKNGKYDANGFFGNNGY

Target ORF information:

RefSeq Version	XM_011488035.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes collagen alpha-1(III) chain (LOC101174706), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011488035.2

ORF Insert Sequence:

ATGGTGAGAC GATGCTTCCA TCTCACTACG GTGATCCTCT GGTTGGTGCA GGCAGTGCAT 
TCGGGGGGAT ATAAACCACA GAATGGCAGA GGTGTGGGGA CATTGCTGAT GCCATCAAAA 
GGTGTGGGTC ATGCAGTGAG GCCACAAAAC GGATATGCTG GATATCCCAT GAAGGGACCA 
GGTTATGGAG CCACAGCTGG GACGAACAAA GGCAGTGGAA TGAAAGGTTA CGGACAAACG 
GCTGGACAGA TGAGTGAACA AGGGGGTAGA CAGCTGGGAT ATGGTGTCAA AGCCGGCCCA 
GTCACTGGAC AGATGAACGG AGGCTTTGGT GCTCAACAAG CATTTGGTGG AGCAGGAGCC 
AAAGGCAATG GAAGAAATCC TGCAGGTTCG TTCAACAGAA ATGGAGTACA ACCTAATGTA 
GGATATGGCG TTTCAGCTGG GCCGTTATCT GGACCACAAA TCAAAGGTTT CAATCCTCAG 
GCTGGTGGAT ATGGAGCGAC GAAAGGAAAT GGTTATGAGG CAGCAGCTGC CTCACAGTAT 
ACACCTGGAA TAAGAGGCAA TGTATATGGA TCTAAAGCTG GCCCAAGCAT CAGACAACTG 
AAATCTGGTT ATGTTGCTCA AACTGGTGGT AATAGTGGGC AAGGAGAAAA ACCCAATGGG 
TATGGTGCTG TGGCTGGCCC AAGCACAGGA CAACAAATGA GAAATGGTTA CAATCTTCAA 
TCTGGAGGAC AAACGACTAA AGGCAATGGT CAAGGCGTGG TGGCTGGACC CTCTACGGCA 
AATGGAGCAT ATGGACAAGG AGGAATGGGA AGCAAACTTA TGAAGGGTTA TGGCCGAACA 
CCTTATGGAT CAGGTGTTCA GAGGGGAGTG TTGGGAGGCC TCGGAGTTCC TCGGCCTGCT 
AGGAACCAGC AGAAATCGTA TGGGAGCAGA ACTGGTGACA GAGGTCAGCC CAACAGCGGC 
TACAATGGCG GTTATGGCAG CACAGCTCTG GGACCTCGTT ATGGAAATGG CGGGATGAAA 
GGACCGGTAA AAGGCTATGC AAACTTACCA CATACTGGTA ATAGAGGAGC AGTTCTAAAC 
GGTTATGAAC AACAAGGCGG TGGAGTAGCT GCAAAACCAG CAAAATCAGG TGGTGGTAGT 
ATTCCTAATG GATATGGTGC CAGACCTAAC GGAAATCCTC ATTTAAATGG AGGTTCAGGG 
AGACCCCAGC CAAGTTTTGG AGCCACAGCT GAAGGCGGAG TTTTCAAAGG ATATGGCACT 
AAACCAAGTG GTCATGGTGG AAATGGTGCT GTTCTGAGTG GATATGGACG ACAGCCCAAC 
GGTTACAACC TGATGCCAAA TGGAAAGGGC CAGGCTGTTA GAGGAACAGA GGTATCAGGT 
GAAAGGAACC TCAAAGGTGG TGTGCACTTC CACCACCATC ATCATCAGCA GCAGCAGATG 
ACGGCACAGT TGGGGGATGT CACCCCACGA GCAGCTACCC CACCTATCCC GGCCCCTACA 
GGTGGTCTCC TTGTTATGGT GACCCAAGAG AAGTACCAAA AGCTGCCTTC ACCTTTGCCC 
CAGGGGAAAA AGTACAAACA CACAGCAATC CTGCCTGAGG AAAGCCCAGA ACCAGCACCT 
GCATATCCTG AAGCCAGAGG GCAGCACCTC TCACTGGAGC CTGCACCTAT TGGTCCAAAG 
GACAAGAATC AGAAGGGGGT CCATTCTGGA CCTGCAGTCG TGGTTCCCCA AAGCAAACCC 
GCACCAGAAC CAGCAGCTGT TCAAGAGAGT AGGGGATCTT CTGCCTCAAA GGGACCAGGA 
GCCAAACTTG ACTGTGGACT GAATGGTCAG TGGATGAAAA TACCAAGACC TGATTACAAT 
GCAGCAGCTG GTAGTTTGGA TGGCTCACAA ACTAAAGGTT ATGGAGCAGG AGAAAATGCT 
TTTCTAAACA ATGGATATGG AGCAGGGCTT GGGTACCCGT ATGCAGGGAA AGCAGCTCAA 
GCTGGATATG GAAAAGGAGT TCACCCTGGG GCTGGATATG AAAATGGAAA TCCATATGGA 
GCTCTCAGTG AAGCAGGCTA TAGAAATGGG TATGGAGCAG GACCTCAGCC TGACTATTCA 
AGTTATGGTC AAGGTGTGCC GACTATTGAT GGCAAATCAG GTGGAAACCC AGGACTTCCT 
GCAGGACTAG ATGGAATGAG TCAGTTTGAG CCTCAGTCTG TTGGTCTGGG TCCAAATGGA 
AAAGGCGTCA TGTATGGTGT TGGGGGGCTG CAGTTTGGTG GACAACCTGG GAACTCCGGG 
ATAAATGGTG CAGGACAGTA TGGAGGTGTC GTTGCAAGTA TGGGAGGGAG TCTTGTGCCT 
GGAATGCACA GTTATGGTGG ATATGCTTAT AACCAAAATG CTCCTGGAAA ACAGGGTTAT 
GGAAGACTTC CTTATGGAGT TCAACAGGCT GGACTTAACC CTGAGGCTAA AGCCGCCGCC 
CAGTATGGTC ATACTGGGTC ATCATATCAG CCTGAATCTG TTGGCTTCGG ACAACCAGGG 
AAAACAGCAG GATCATACAG CGGGGGACAG GCTGCCTTTG CACCTCAGGC TCTTGGGTTT 
GGGGGTGATG ATAAATCTGG TAAATATGAT AAACAGGGAG CTTTCCAATC ACAGCGTCTC 
CGGCCTGAAG AAACAGCCGG TCAGACATAT GAGCGCCAGT CTGTTGGGTT GGATTCAGCA 
GGAAAGTCCT ATGTGAAAGG GGAAGTGCCA ACTGCAACTC TAGCAGCTGA AAGTGAGAGG 
ATTCCCATTA ATAAATATGA AAATGTGGGC TACATAAATG GACAAATGCA GCCAGAAGTT 
GCTGGGCTTC CTGCAGCTCC CACTGCCAGT TCCACCCTGG CCTTTTCCTC TCCTGATGGG 
ATTCCTGTAG AGGATGTTCA GGACATTCCT GATCCAGAGG GCACAGCCAG CTTCTTGGAG 
TCGGGACCCG TTACAGAAAT GCTGGGCGAG TCTCGGGGAC CGGAGGATGC CGAGAACCTG 
CCACGTCAGA TACACATCCA GCAGCATCTC AAACTCCATT TCAACCCACA AGGCAAAAAA 
AATGGCAAAT ATGATGCAAA TGGCTTCTTT GGGAACAATG GCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101174706 ( XM_020712199.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020567858.1 Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OJa74382
Clone ID Related Accession (Same CDS sequence)	XM_020712199.1
Accession Version	XM_020712199.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	fibroin heavy chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019877.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGAC GATGCTTCCA TCTCACTACG GTGATCCTCT GGTTGGTGCA GGCAGTGCAT 
TCGGGGGGAT ATAAACCACA GAATGGCAGA GGTGTGGGGA CATTGCTGAT GCCATCAAAA 
GGTGTGGGTC ATGCAGTGAG GCCACAAAAC GGATATGCTG GATATCCCAT GAAGGGACCA 
GGTTATGGAG CCACAGCTGG GACGAACAAA GGCAGTGGAA TGAAAGGTTA CGGACAAACG 
GCTGGACAGA TGAGTGAACA AGGGGGTAGA CAGCTGGGAT ATGGTGTCAA AGCCGGCCCA 
GTCACTGGAC AGATGAACGG AGGCTTTGGT GCTCAACAAG CATTTGGTGG AGCAGGAGCC 
AAAGGCAATG GAAGAAATCC TGCAGGTTCG TTCAACAGAA ATGGAGTACA ACCTAATGTA 
GGATATGGCG TTTCAGCTGG GCCGTTATCT GGACCACAAA TCAAAGGTTT CAATCCTCAG 
GCTGGTGGAT ATGGAGCGAC GAAAGGAAAT GGTTATGAGG CAGCAGCTGC CTCACAGTAT 
ACACCTGGAA TAAGAGGCAA TGTATATGGA TCTAAAGCTG GCCCAAGCAT CAGACAACTG 
AAATCTGGTT ATGTTGCTCA AACTGGTGGT AATAGTGGGC AAGGAGAAAA ACCCAATGGG 
TATGGTGCTG TGGCTGGCCC AAGCACAGGA CAACAAATGA GAAATGGTTA CAATCTTCAA 
TCTGGAGGAC AAACGACTAA AGGCAATGGT CAAGGCGTGG TGGCTGGACC CTCTACGGCA 
AATGGAGCAT ATGGACAAGG AGGAATGGGA AGCAAACTTA TGAAGGGTTA TGGCCGAACA 
CCTTATGGAT CAGGTGTTCA GAGGGGAGTG TTGGGAGGCC TCGGAGTTCC TCGGCCTGCT 
AGGAACCAGC AGAAATCGTA TGGGAGCAGA ACTGGTGACA GAGGTCAGCC CAACAGCGGC 
TACAATGGCG GTTATGGCAG CACAGCTCTG GGACCTCGTT ATGGAAATGG CGGGATGAAA 
GGACCGGTAA AAGGCTATGC AAACTTACCA CATACTGGTA ATAGAGGAGC AGTTCTAAAC 
GGTTATGAAC AACAAGGCGG TGGAGTAGCT GCAAAACCAG CAAAATCAGG TGGTGGTAGT 
ATTCCTAATG GATATGGTGC CAGACCTAAC GGAAATCCTC ATTTAAATGG AGGTTCAGGG 
AGACCCCAGC CAAGTTTTGG AGCCACAGCT GAAGGCGGAG TTTTCAAAGG ATATGGCACT 
AAACCAAGTG GTCATGGTGG AAATGGTGCT GTTCTGAGTG GATATGGACG ACAGCCCAAC 
GGTTACAACC TGATGCCAAA TGGAAAGGGC CAGGCTGTTA GAGGAACAGA GGTATCAGGT 
GAAAGGAACC TCAAAGGTGG TGTGCACTTC CACCACCATC ATCATCAGCA GCAGCAGATG 
ACGGCACAGT TGGGGGATGT CACCCCACGA GCAGCTACCC CACCTATCCC GGCCCCTACA 
GGTGGTCTCC TTGTTATGGT GACCCAAGAG AAGTACCAAA AGCTGCCTTC ACCTTTGCCC 
CAGGGGAAAA AGTACAAACA CACAGCAATC CTGCCTGAGG AAAGCCCAGA ACCAGCACCT 
GCATATCCTG AAGCCAGAGG GCAGCACCTC TCACTGGAGC CTGCACCTAT TGGTCCAAAG 
GACAAGAATC AGAAGGGGGT CCATTCTGGA CCTGCAGTCG TGGTTCCCCA AAGCAAACCC 
GCACCAGAAC CAGCAGCTGT TCAAGGTTAT GTGACTGGAA ATGCTGTTCA TCTGCAAAAA 
GCTGCAGAAG CAGACGCTGT AGGTTTGGGG GGGGGCGCCC CAAAAACCAA AGCTGTTCCT 
GAAACAGCTG AGGAAAGGGG CGGTGTCCCT CCAAAGGAAA CACTGATCAC TGCGGGTGTG 
GGCGAGTCTG CAGCAGAGGA GGCTGACGCA CAACCTGACA GGCTGCAGGG CACAAGTATC 
CATGCTGGGG AGACGGGTAC TGAACCACAC CTAGGTTTGG AGAGTAGGGG ATCTTCTGCC 
TCAAAGGGAC CAGGAGCCAA ACTTGACTGT GGACTGAATG GTCAGTGGAT GAAAATACCA 
AGACCTGATT ACAATGCAGC AGCTGGTAGT TTGGATGGCT CACAAACTAA AGGTTATGGA 
GCAGGAGAAA ATGCTTTTCT AAACAATGGA TATGGAGCAG GGCTTGGGTA CCCGTATGCA 
GGGAAAGCAG CTCAAGCTGG ATATGGAAAA GGAGTTCACC CTGGGGCTGG ATATGAAAAT 
GGAAATCCAT ATGGAGCTCT CAGTGAAGCA GGCTATAGAA ATGGGTATGG AGCAGGACCT 
CAGCCTGACT ATTCAAGTTA TGGTCAAGGT GTGCCGACTA TTGATGGCAA ATCAGGTGGA 
AACCCAGGAC TTCCTGCAGG ACTAGATGGA ATGAGTCAGT TTGAGCCTCA GTCTGTTGGT 
CTGGGTCCAA ATGGAAAAGG CGTCATGTAT GGTGTTGGGG GGCTGCAGTT TGGTGGACAA 
CCTGGGAACT CCGGGATAAA TGGTGCAGGA CAGTATGGAG GTGTCGTTGC AAGTATGGGA 
GGGAGTCTTG TGCCTGGAAT GCACAGTTAT GGTGGATATG CTTATAACCA AAATGCTCCT 
GGAAAACAGG GTTATGGAAG ACTTCCTTAT GGAGTTCAAC AGGCTGGACT TAACCCTGAG 
GCTAAAGCCG CCGCCCAGTA TGGTCATACT GGGTCATCAT ATCAGCCTGA ATCTGTTGGC 
TTCGGACAAC CAGGGAAAAC AGCAGGATCA TACAGCGGGG GACAGGCTGC CTTTGCACCT 
CAGGCTCTTG GGTTTGGGGG TGATGATAAA TCTGGTAAAT ATGATAAACA GGGAGCTTTC 
CAATCACAGC GTCTCCGGCC TGAAGAAACA GCCGGTCAGA CATATGAGCG CCAGTCTGTT 
GGGTTGGATT CAGCAGGAAA GTCCTATGTG AAAGGGGAAG TGCCAACTGC AACTCTAGCA 
GCTGAAAGTG AGAGGATTCC CATTAATAAA TATGAAAATG TGGGCTACAT AAATGGACAA 
ATGCAGCCAG AAGTTGCTGG GCTTCCTGCA GCTCCCACTG CCAGTTCCAC CCTGGCCTTT 
TCCTCTCCTG ATGGGATTCC TGTAGAGGAT GTTCAGGACA TTCCTGATCC AGAGGGCACA 
GCCAGCTTCT TGGAGTCGGG ACCCGTTACA GAAATGCTGG GCGAGTCTCG GGGACCGGAG 
GATGCCGAGA ACCTGCCACG TCAGATACAC ATCCAGCAGC ATCTCAAACT CCATTTCAAC 
CCACAAGGCA AAAAAAATGG CAAATATGAT GCAAATGGCT TCTTTGGGAA CAATGGCTAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020567858.1
CDS	113..3475
Translation	MVRRCFHLTTVILWLVQAVHSGGYKPQNGRGVGTLLMPSKGVGHAVRPQNGYAGYPMKGPGYGATAGTNKGSGMKGYGQTAGQMSEQGGRQLGYGVKAGPVTGQMNGGFGAQQAFGGAGAKGNGRNPAGSFNRNGVQPNVGYGVSAGPLSGPQIKGFNPQAGGYGATKGNGYEAAAASQYTPGIRGNVYGSKAGPSIRQLKSGYVAQTGGNSGQGEKPNGYGAVAGPSTGQQMRNGYNLQSGGQTTKGNGQGVVAGPSTANGAYGQGGMGSKLMKGYGRTPYGSGVQRGVLGGLGVPRPARNQQKSYGSRTGDRGQPNSGYNGGYGSTALGPRYGNGGMKGPVKGYANLPHTGNRGAVLNGYEQQGGGVAAKPAKSGGGSIPNGYGARPNGNPHLNGGSGRPQPSFGATAEGGVFKGYGTKPSGHGGNGAVLSGYGRQPNGYNLMPNGKGQAVRGTEVSGERNLKGGVHFHHHHHQQQQMTAQLGDVTPRAATPPIPAPTGGLLVMVTQEKYQKLPSPLPQGKKYKHTAILPEESPEPAPAYPEARGQHLSLEPAPIGPKDKNQKGVHSGPAVVVPQSKPAPEPAAVQGYVTGNAVHLQKAAEADAVGLGGGAPKTKAVPETAEERGGVPPKETLITAGVGESAAEEADAQPDRLQGTSIHAGETGTEPHLGLESRGSSASKGPGAKLDCGLNGQWMKIPRPDYNAAAGSLDGSQTKGYGAGENAFLNNGYGAGLGYPYAGKAAQAGYGKGVHPGAGYENGNPYGALSEAGYRNGYGAGPQPDYSSYGQGVPTIDGKSGGNPGLPAGLDGMSQFEPQSVGLGPNGKGVMYGVGGLQFGGQPGNSGINGAGQYGGVVASMGGSLVPGMHSYGGYAYNQNAPGKQGYGRLPYGVQQAGLNPEAKAAAQYGHTGSSYQPESVGFGQPGKTAGSYSGGQAAFAPQALGFGGDDKSGKYDKQGAFQSQRLRPEETAGQTYERQSVGLDSAGKSYVKGEVPTATLAAESERIPINKYENVGYINGQMQPEVAGLPAAPTASSTLAFSSPDGIPVEDVQDIPDPEGTASFLESGPVTEMLGESRGPEDAENLPRQIHIQQHLKLHFNPQGKKNGKYDANGFFGNNGY

Target ORF information:

RefSeq Version	XM_020712199.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020712199.1

ORF Insert Sequence:

ATGGTGAGAC GATGCTTCCA TCTCACTACG GTGATCCTCT GGTTGGTGCA GGCAGTGCAT 
TCGGGGGGAT ATAAACCACA GAATGGCAGA GGTGTGGGGA CATTGCTGAT GCCATCAAAA 
GGTGTGGGTC ATGCAGTGAG GCCACAAAAC GGATATGCTG GATATCCCAT GAAGGGACCA 
GGTTATGGAG CCACAGCTGG GACGAACAAA GGCAGTGGAA TGAAAGGTTA CGGACAAACG 
GCTGGACAGA TGAGTGAACA AGGGGGTAGA CAGCTGGGAT ATGGTGTCAA AGCCGGCCCA 
GTCACTGGAC AGATGAACGG AGGCTTTGGT GCTCAACAAG CATTTGGTGG AGCAGGAGCC 
AAAGGCAATG GAAGAAATCC TGCAGGTTCG TTCAACAGAA ATGGAGTACA ACCTAATGTA 
GGATATGGCG TTTCAGCTGG GCCGTTATCT GGACCACAAA TCAAAGGTTT CAATCCTCAG 
GCTGGTGGAT ATGGAGCGAC GAAAGGAAAT GGTTATGAGG CAGCAGCTGC CTCACAGTAT 
ACACCTGGAA TAAGAGGCAA TGTATATGGA TCTAAAGCTG GCCCAAGCAT CAGACAACTG 
AAATCTGGTT ATGTTGCTCA AACTGGTGGT AATAGTGGGC AAGGAGAAAA ACCCAATGGG 
TATGGTGCTG TGGCTGGCCC AAGCACAGGA CAACAAATGA GAAATGGTTA CAATCTTCAA 
TCTGGAGGAC AAACGACTAA AGGCAATGGT CAAGGCGTGG TGGCTGGACC CTCTACGGCA 
AATGGAGCAT ATGGACAAGG AGGAATGGGA AGCAAACTTA TGAAGGGTTA TGGCCGAACA 
CCTTATGGAT CAGGTGTTCA GAGGGGAGTG TTGGGAGGCC TCGGAGTTCC TCGGCCTGCT 
AGGAACCAGC AGAAATCGTA TGGGAGCAGA ACTGGTGACA GAGGTCAGCC CAACAGCGGC 
TACAATGGCG GTTATGGCAG CACAGCTCTG GGACCTCGTT ATGGAAATGG CGGGATGAAA 
GGACCGGTAA AAGGCTATGC AAACTTACCA CATACTGGTA ATAGAGGAGC AGTTCTAAAC 
GGTTATGAAC AACAAGGCGG TGGAGTAGCT GCAAAACCAG CAAAATCAGG TGGTGGTAGT 
ATTCCTAATG GATATGGTGC CAGACCTAAC GGAAATCCTC ATTTAAATGG AGGTTCAGGG 
AGACCCCAGC CAAGTTTTGG AGCCACAGCT GAAGGCGGAG TTTTCAAAGG ATATGGCACT 
AAACCAAGTG GTCATGGTGG AAATGGTGCT GTTCTGAGTG GATATGGACG ACAGCCCAAC 
GGTTACAACC TGATGCCAAA TGGAAAGGGC CAGGCTGTTA GAGGAACAGA GGTATCAGGT 
GAAAGGAACC TCAAAGGTGG TGTGCACTTC CACCACCATC ATCATCAGCA GCAGCAGATG 
ACGGCACAGT TGGGGGATGT CACCCCACGA GCAGCTACCC CACCTATCCC GGCCCCTACA 
GGTGGTCTCC TTGTTATGGT GACCCAAGAG AAGTACCAAA AGCTGCCTTC ACCTTTGCCC 
CAGGGGAAAA AGTACAAACA CACAGCAATC CTGCCTGAGG AAAGCCCAGA ACCAGCACCT 
GCATATCCTG AAGCCAGAGG GCAGCACCTC TCACTGGAGC CTGCACCTAT TGGTCCAAAG 
GACAAGAATC AGAAGGGGGT CCATTCTGGA CCTGCAGTCG TGGTTCCCCA AAGCAAACCC 
GCACCAGAAC CAGCAGCTGT TCAAGGTTAT GTGACTGGAA ATGCTGTTCA TCTGCAAAAA 
GCTGCAGAAG CAGACGCTGT AGGTTTGGGG GGGGGCGCCC CAAAAACCAA AGCTGTTCCT 
GAAACAGCTG AGGAAAGGGG CGGTGTCCCT CCAAAGGAAA CACTGATCAC TGCGGGTGTG 
GGCGAGTCTG CAGCAGAGGA GGCTGACGCA CAACCTGACA GGCTGCAGGG CACAAGTATC 
CATGCTGGGG AGACGGGTAC TGAACCACAC CTAGGTTTGG AGAGTAGGGG ATCTTCTGCC 
TCAAAGGGAC CAGGAGCCAA ACTTGACTGT GGACTGAATG GTCAGTGGAT GAAAATACCA 
AGACCTGATT ACAATGCAGC AGCTGGTAGT TTGGATGGCT CACAAACTAA AGGTTATGGA 
GCAGGAGAAA ATGCTTTTCT AAACAATGGA TATGGAGCAG GGCTTGGGTA CCCGTATGCA 
GGGAAAGCAG CTCAAGCTGG ATATGGAAAA GGAGTTCACC CTGGGGCTGG ATATGAAAAT 
GGAAATCCAT ATGGAGCTCT CAGTGAAGCA GGCTATAGAA ATGGGTATGG AGCAGGACCT 
CAGCCTGACT ATTCAAGTTA TGGTCAAGGT GTGCCGACTA TTGATGGCAA ATCAGGTGGA 
AACCCAGGAC TTCCTGCAGG ACTAGATGGA ATGAGTCAGT TTGAGCCTCA GTCTGTTGGT 
CTGGGTCCAA ATGGAAAAGG CGTCATGTAT GGTGTTGGGG GGCTGCAGTT TGGTGGACAA 
CCTGGGAACT CCGGGATAAA TGGTGCAGGA CAGTATGGAG GTGTCGTTGC AAGTATGGGA 
GGGAGTCTTG TGCCTGGAAT GCACAGTTAT GGTGGATATG CTTATAACCA AAATGCTCCT 
GGAAAACAGG GTTATGGAAG ACTTCCTTAT GGAGTTCAAC AGGCTGGACT TAACCCTGAG 
GCTAAAGCCG CCGCCCAGTA TGGTCATACT GGGTCATCAT ATCAGCCTGA ATCTGTTGGC 
TTCGGACAAC CAGGGAAAAC AGCAGGATCA TACAGCGGGG GACAGGCTGC CTTTGCACCT 
CAGGCTCTTG GGTTTGGGGG TGATGATAAA TCTGGTAAAT ATGATAAACA GGGAGCTTTC 
CAATCACAGC GTCTCCGGCC TGAAGAAACA GCCGGTCAGA CATATGAGCG CCAGTCTGTT 
GGGTTGGATT CAGCAGGAAA GTCCTATGTG AAAGGGGAAG TGCCAACTGC AACTCTAGCA 
GCTGAAAGTG AGAGGATTCC CATTAATAAA TATGAAAATG TGGGCTACAT AAATGGACAA 
ATGCAGCCAG AAGTTGCTGG GCTTCCTGCA GCTCCCACTG CCAGTTCCAC CCTGGCCTTT 
TCCTCTCCTG ATGGGATTCC TGTAGAGGAT GTTCAGGACA TTCCTGATCC AGAGGGCACA 
GCCAGCTTCT TGGAGTCGGG ACCCGTTACA GAAATGCTGG GCGAGTCTCG GGGACCGGAG 
GATGCCGAGA ACCTGCCACG TCAGATACAC ATCCAGCAGC ATCTCAAACT CCATTTCAAC 
CCACAAGGCA AAAAAAATGG CAAATATGAT GCAAATGGCT TCTTTGGGAA CAATGGCTAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101174706 ( XM_020712200.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020567859.1 Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OJa74383
Clone ID Related Accession (Same CDS sequence)	XM_020712200.1
Accession Version	XM_020712200.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	fibroin heavy chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019877.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGAC GATGCTTCCA TCTCACTACG GTGATCCTCT GGTTGGTGCA GGCAGTGCAT 
TCGGGGGGAT ATAAACCACA GAATGGCAGA GGTGTGGGGA CATTGCTGAT GCCATCAAAA 
GGTGTGGGTC ATGCAGTGAG GCCACAAAAC GGATATGCTG GATATCCCAT GAAGGGACCA 
GGTTATGGAG CCACAGCTGG GACGAACAAA GGCAGTGGAA TGAAAGGTTA CGGACAAACG 
GCTGGACAGA TGAGTGAACA AGGGGGTAGA CAGCTGGGAT ATGGTGTCAA AGCCGGCCCA 
GTCACTGGAC AGATGAACGG AGGCTTTGGT GCTCAACAAG CATTTGGTGG AGCAGGAGCC 
AAAGGCAATG GAAGAAATCC TGCAGGTTCG TTCAACAGAA ATGGAGTACA ACCTAATGTA 
GGATATGGCG TTTCAGCTGG GCCGTTATCT GGACCACAAA TCAAAGGTTT CAATCCTCAG 
GCTGGTGGAT ATGGAGCGAC GAAAGGAAAT GGTTATGAGG CAGCAGCTGC CTCACAGTAT 
ACACCTGGAA TAAGAGGCAA TGTATATGGA TCTAAAGCTG GCCCAAGCAT CAGACAACTG 
AAATCTGGTT ATGTTGCTCA AACTGGTGGT AATAGTGGGC AAGGAGAAAA ACCCAATGGG 
TATGGTGCTG TGGCTGGCCC AAGCACAGGA CAACAAATGA GAAATGGTTA CAATCTTCAA 
TCTGGAGGAC AAACGACTAA AGGCAATGGT CAAGGCGTGG TGGCTGGACC CTCTACGGCA 
AATGGAGCAT ATGGACAAGG AGGAATGGGA AGCAAACTTA TGAAGGGTTA TGGCCGAACA 
CCTTATGGAT CAGGTGTTCA GAGGGGAGTG TTGGGAGGCC TCGGAGTTCC TCGGCCTGCT 
AGGAACCAGC AGAAATCGTA TGGGAGCAGA ACTGGTGACA GAGGTCAGCC CAACAGCGGC 
TACAATGGCG GTTATGGCAG CACAGCTCTG GGACCTCGTT ATGGAAATGG CGGGATGAAA 
GGACCGGTAA AAGGCTATGC AAACTTACCA CATACTGGTA ATAGAGGAGC AGTTCTAAAC 
GGTTATGAAC AACAAGGCGG TGGAGTAGCT GCAAAACCAG CAAAATCAGG TGGTGGTAGT 
ATTCCTAATG GATATGGTGC CAGACCTAAC GGAAATCCTC ATTTAAATGG AGGTTCAGGG 
AGACCCCAGC CAAGTTTTGG AGCCACAGCT GAAGGCGGAG TTTTCAAAGG ATATGGCACT 
AAACCAAGTG GTCATGGTGG AAATGGTGCT GTTCTGAGTG GATATGGACG ACAGCCCAAC 
GGTTACAACC TGATGCCAAA TGGAAAGGGC CAGGCTGTTA GAGGAACAGA GGTATCAGGT 
GAAAGGAACC TCAAAGGTGG TGTGCACTTC CACCACCATC ATCATCAGCA GCAGCAGATG 
ACGGCACAGT TGGGGGATGT CACCCCACGA GCAGCTACCC CACCTATCCC GGCCCCTACA 
GGTGGTCTCC TTGTTATGGT GACCCAAGAG AAGTACCAAA AGCTGCCTTC ACCTTTGCCC 
CAGGGGAAAA AGTACAAACA CACAGCAATC CTGCCTGAGG AAAGCCCAGA ACCAGCACCT 
GCATATCCTG AAGCCAGAGG GCAGCACCTC TCACTGGAGC CTGCACCTAT TGGTCCAAAG 
GACAAGAATC AGAAGGGGGT CCATTCTGGA CCTGCAGTCG TGGTTCCCCA AAGCAAACCC 
GCACCAGAAC CAGCAGCTGT TCAAGGTTAT GTGACTGGAA ATGCTGTTCA TCTGCAAAAA 
GCTGCAGAAG CAGACGCTGT AGGTTTGGGG GGGGGCGCCC CAAAAACCAA AGCTGTTCCT 
GAAACAGCTG AGGAAAGGGG CGGTGTCCCT CCAAAGGAAA CACTGATCAC TGCGGGTGTG 
GGCGAGTCTG CAGCAGAGGA GGCTGACGCA CAACCTGACA GGCTGCAGGG CACAAGTATC 
CATGCTGGGG AGACGGGTAC TGAACCACAC CTAGGTTTGG AGAGTAGGGG ATCTTCTGCC 
TCAAAGGGAC CAGGAGCCAA ACTTGACTGT GGACTGAATG GTCAGTGGAT GAAAATACCA 
AGACCTGATT ACAATGCAGC AGCTGGTAGT TTGGATGGCT CACAAACTAA AGGTTATGGA 
GCAGGAGAAA ATGCTTTTCT AAACAATGGA TATGGAGCAG GGCTTGGGTA CCCGTATGCA 
GGGAAAGCAG CTCAAGCTGG ATATGGAAAA GGAGTTCACC CTGGGGCTGG ATATGAAAAT 
GGAAATCCAT ATGGAGCTCT CAGTGAAGCA GGCTATAGAA ATGGGTATGG AGCAGGACCT 
CAGCCTGACT ATTCAAGTTA TGGTCAAGGT GTGCCGACTA TTGATGGCAA ATCAGGTGGA 
AACCCAGGAC TTCCTGCAGG ACTAGATGGA ATGAGTCAGT TTGAGCCTCA GTCTGTTGGT 
CTGGGTCCAA ATGGAAAAGG CGTCATGTAT GGTGTTGGGG GGCTGCAGTT TGGTGGACAA 
CCTGGGAACT CCGGGATAAA TGGTGCAGGA CAGTATGGAG GTGTCGTTGC AAGTATGGGA 
GGGAGTCTTG TGCCTGGAAT GCACAGTTAT GGTGGATATG CTTATAACCA AAATGCTCCT 
GGAAAACAGG GTTATGGAAG ACTTCCTTAT GGAGTTCAAC AGGCTGGACT TAACCCTGAG 
GCTAAAGCCG CCGCCCAGTA TGGTCATACT GGGTCATCAT ATCAGCCTGA ATCTGTTGGC 
TTCGGACAAC CAGGGAAAAC AGCAGGATCA TACAGCGGGG GACAGGCTGC CTTTGCACCT 
CAGGCTCTTG GGTTTGGGGG TGATGATAAA TCTGATAAAC AGGGAGCTTT CCAATCACAG 
CGTCTCCGGC CTGAAGAAAC AGCCGGTCAG ACATATGAGC GCCAGTCTGT TGGGTTGGAT 
TCAGCAGGAA AGTCCTATGT GAAAGGGGAA GTGCCAACTG CAACTCTAGC AGCTGAAAGT 
GAGAGGATTC CCATTAATAA ATATGAAAAT GTGGGCTACA TAAATGGACA AATGCAGCCA 
GAAGTTGCTG GGCTTCCTGC AGCTCCCACT GCCAGTTCCA CCCTGGCCTT TTCCTCTCCT 
GATGGGATTC CTGTAGAGGA TGTTCAGGAC ATTCCTGATC CAGAGGGCAC AGCCAGCTTC 
TTGGAGTCGG GACCCGTTAC AGAAATGCTG GGCGAGTCTC GGGGACCGGA GGATGCCGAG 
AACCTGCCAC GTCAGATACA CATCCAGCAG CATCTCAAAC TCCATTTCAA CCCACAAGGC 
AAAAAAAATG GCAAATATGA TGCAAATGGC TTCTTTGGGA ACAATGGCTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020567859.1
CDS	113..3466
Translation	MVRRCFHLTTVILWLVQAVHSGGYKPQNGRGVGTLLMPSKGVGHAVRPQNGYAGYPMKGPGYGATAGTNKGSGMKGYGQTAGQMSEQGGRQLGYGVKAGPVTGQMNGGFGAQQAFGGAGAKGNGRNPAGSFNRNGVQPNVGYGVSAGPLSGPQIKGFNPQAGGYGATKGNGYEAAAASQYTPGIRGNVYGSKAGPSIRQLKSGYVAQTGGNSGQGEKPNGYGAVAGPSTGQQMRNGYNLQSGGQTTKGNGQGVVAGPSTANGAYGQGGMGSKLMKGYGRTPYGSGVQRGVLGGLGVPRPARNQQKSYGSRTGDRGQPNSGYNGGYGSTALGPRYGNGGMKGPVKGYANLPHTGNRGAVLNGYEQQGGGVAAKPAKSGGGSIPNGYGARPNGNPHLNGGSGRPQPSFGATAEGGVFKGYGTKPSGHGGNGAVLSGYGRQPNGYNLMPNGKGQAVRGTEVSGERNLKGGVHFHHHHHQQQQMTAQLGDVTPRAATPPIPAPTGGLLVMVTQEKYQKLPSPLPQGKKYKHTAILPEESPEPAPAYPEARGQHLSLEPAPIGPKDKNQKGVHSGPAVVVPQSKPAPEPAAVQGYVTGNAVHLQKAAEADAVGLGGGAPKTKAVPETAEERGGVPPKETLITAGVGESAAEEADAQPDRLQGTSIHAGETGTEPHLGLESRGSSASKGPGAKLDCGLNGQWMKIPRPDYNAAAGSLDGSQTKGYGAGENAFLNNGYGAGLGYPYAGKAAQAGYGKGVHPGAGYENGNPYGALSEAGYRNGYGAGPQPDYSSYGQGVPTIDGKSGGNPGLPAGLDGMSQFEPQSVGLGPNGKGVMYGVGGLQFGGQPGNSGINGAGQYGGVVASMGGSLVPGMHSYGGYAYNQNAPGKQGYGRLPYGVQQAGLNPEAKAAAQYGHTGSSYQPESVGFGQPGKTAGSYSGGQAAFAPQALGFGGDDKSDKQGAFQSQRLRPEETAGQTYERQSVGLDSAGKSYVKGEVPTATLAAESERIPINKYENVGYINGQMQPEVAGLPAAPTASSTLAFSSPDGIPVEDVQDIPDPEGTASFLESGPVTEMLGESRGPEDAENLPRQIHIQQHLKLHFNPQGKKNGKYDANGFFGNNGY

Target ORF information:

RefSeq Version	XM_020712200.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020712200.1

ORF Insert Sequence:

ATGGTGAGAC GATGCTTCCA TCTCACTACG GTGATCCTCT GGTTGGTGCA GGCAGTGCAT 
TCGGGGGGAT ATAAACCACA GAATGGCAGA GGTGTGGGGA CATTGCTGAT GCCATCAAAA 
GGTGTGGGTC ATGCAGTGAG GCCACAAAAC GGATATGCTG GATATCCCAT GAAGGGACCA 
GGTTATGGAG CCACAGCTGG GACGAACAAA GGCAGTGGAA TGAAAGGTTA CGGACAAACG 
GCTGGACAGA TGAGTGAACA AGGGGGTAGA CAGCTGGGAT ATGGTGTCAA AGCCGGCCCA 
GTCACTGGAC AGATGAACGG AGGCTTTGGT GCTCAACAAG CATTTGGTGG AGCAGGAGCC 
AAAGGCAATG GAAGAAATCC TGCAGGTTCG TTCAACAGAA ATGGAGTACA ACCTAATGTA 
GGATATGGCG TTTCAGCTGG GCCGTTATCT GGACCACAAA TCAAAGGTTT CAATCCTCAG 
GCTGGTGGAT ATGGAGCGAC GAAAGGAAAT GGTTATGAGG CAGCAGCTGC CTCACAGTAT 
ACACCTGGAA TAAGAGGCAA TGTATATGGA TCTAAAGCTG GCCCAAGCAT CAGACAACTG 
AAATCTGGTT ATGTTGCTCA AACTGGTGGT AATAGTGGGC AAGGAGAAAA ACCCAATGGG 
TATGGTGCTG TGGCTGGCCC AAGCACAGGA CAACAAATGA GAAATGGTTA CAATCTTCAA 
TCTGGAGGAC AAACGACTAA AGGCAATGGT CAAGGCGTGG TGGCTGGACC CTCTACGGCA 
AATGGAGCAT ATGGACAAGG AGGAATGGGA AGCAAACTTA TGAAGGGTTA TGGCCGAACA 
CCTTATGGAT CAGGTGTTCA GAGGGGAGTG TTGGGAGGCC TCGGAGTTCC TCGGCCTGCT 
AGGAACCAGC AGAAATCGTA TGGGAGCAGA ACTGGTGACA GAGGTCAGCC CAACAGCGGC 
TACAATGGCG GTTATGGCAG CACAGCTCTG GGACCTCGTT ATGGAAATGG CGGGATGAAA 
GGACCGGTAA AAGGCTATGC AAACTTACCA CATACTGGTA ATAGAGGAGC AGTTCTAAAC 
GGTTATGAAC AACAAGGCGG TGGAGTAGCT GCAAAACCAG CAAAATCAGG TGGTGGTAGT 
ATTCCTAATG GATATGGTGC CAGACCTAAC GGAAATCCTC ATTTAAATGG AGGTTCAGGG 
AGACCCCAGC CAAGTTTTGG AGCCACAGCT GAAGGCGGAG TTTTCAAAGG ATATGGCACT 
AAACCAAGTG GTCATGGTGG AAATGGTGCT GTTCTGAGTG GATATGGACG ACAGCCCAAC 
GGTTACAACC TGATGCCAAA TGGAAAGGGC CAGGCTGTTA GAGGAACAGA GGTATCAGGT 
GAAAGGAACC TCAAAGGTGG TGTGCACTTC CACCACCATC ATCATCAGCA GCAGCAGATG 
ACGGCACAGT TGGGGGATGT CACCCCACGA GCAGCTACCC CACCTATCCC GGCCCCTACA 
GGTGGTCTCC TTGTTATGGT GACCCAAGAG AAGTACCAAA AGCTGCCTTC ACCTTTGCCC 
CAGGGGAAAA AGTACAAACA CACAGCAATC CTGCCTGAGG AAAGCCCAGA ACCAGCACCT 
GCATATCCTG AAGCCAGAGG GCAGCACCTC TCACTGGAGC CTGCACCTAT TGGTCCAAAG 
GACAAGAATC AGAAGGGGGT CCATTCTGGA CCTGCAGTCG TGGTTCCCCA AAGCAAACCC 
GCACCAGAAC CAGCAGCTGT TCAAGGTTAT GTGACTGGAA ATGCTGTTCA TCTGCAAAAA 
GCTGCAGAAG CAGACGCTGT AGGTTTGGGG GGGGGCGCCC CAAAAACCAA AGCTGTTCCT 
GAAACAGCTG AGGAAAGGGG CGGTGTCCCT CCAAAGGAAA CACTGATCAC TGCGGGTGTG 
GGCGAGTCTG CAGCAGAGGA GGCTGACGCA CAACCTGACA GGCTGCAGGG CACAAGTATC 
CATGCTGGGG AGACGGGTAC TGAACCACAC CTAGGTTTGG AGAGTAGGGG ATCTTCTGCC 
TCAAAGGGAC CAGGAGCCAA ACTTGACTGT GGACTGAATG GTCAGTGGAT GAAAATACCA 
AGACCTGATT ACAATGCAGC AGCTGGTAGT TTGGATGGCT CACAAACTAA AGGTTATGGA 
GCAGGAGAAA ATGCTTTTCT AAACAATGGA TATGGAGCAG GGCTTGGGTA CCCGTATGCA 
GGGAAAGCAG CTCAAGCTGG ATATGGAAAA GGAGTTCACC CTGGGGCTGG ATATGAAAAT 
GGAAATCCAT ATGGAGCTCT CAGTGAAGCA GGCTATAGAA ATGGGTATGG AGCAGGACCT 
CAGCCTGACT ATTCAAGTTA TGGTCAAGGT GTGCCGACTA TTGATGGCAA ATCAGGTGGA 
AACCCAGGAC TTCCTGCAGG ACTAGATGGA ATGAGTCAGT TTGAGCCTCA GTCTGTTGGT 
CTGGGTCCAA ATGGAAAAGG CGTCATGTAT GGTGTTGGGG GGCTGCAGTT TGGTGGACAA 
CCTGGGAACT CCGGGATAAA TGGTGCAGGA CAGTATGGAG GTGTCGTTGC AAGTATGGGA 
GGGAGTCTTG TGCCTGGAAT GCACAGTTAT GGTGGATATG CTTATAACCA AAATGCTCCT 
GGAAAACAGG GTTATGGAAG ACTTCCTTAT GGAGTTCAAC AGGCTGGACT TAACCCTGAG 
GCTAAAGCCG CCGCCCAGTA TGGTCATACT GGGTCATCAT ATCAGCCTGA ATCTGTTGGC 
TTCGGACAAC CAGGGAAAAC AGCAGGATCA TACAGCGGGG GACAGGCTGC CTTTGCACCT 
CAGGCTCTTG GGTTTGGGGG TGATGATAAA TCTGATAAAC AGGGAGCTTT CCAATCACAG 
CGTCTCCGGC CTGAAGAAAC AGCCGGTCAG ACATATGAGC GCCAGTCTGT TGGGTTGGAT 
TCAGCAGGAA AGTCCTATGT GAAAGGGGAA GTGCCAACTG CAACTCTAGC AGCTGAAAGT 
GAGAGGATTC CCATTAATAA ATATGAAAAT GTGGGCTACA TAAATGGACA AATGCAGCCA 
GAAGTTGCTG GGCTTCCTGC AGCTCCCACT GCCAGTTCCA CCCTGGCCTT TTCCTCTCCT 
GATGGGATTC CTGTAGAGGA TGTTCAGGAC ATTCCTGATC CAGAGGGCAC AGCCAGCTTC 
TTGGAGTCGG GACCCGTTAC AGAAATGCTG GGCGAGTCTC GGGGACCGGA GGATGCCGAG 
AACCTGCCAC GTCAGATACA CATCCAGCAG CATCTCAAAC TCCATTTCAA CCCACAAGGC 
AAAAAAAATG GCAAATATGA TGCAAATGGC TTCTTTGGGA ACAATGGCTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101174706 ( XM_020712201.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020567860.1 Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X3, mRNA.

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CloneID	OJa74384
Clone ID Related Accession (Same CDS sequence)	XM_020712201.1
Accession Version	XM_020712201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	fibroin heavy chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019877.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGAC GATGCTTCCA TCTCACTACG GTGATCCTCT GGTTGGTGCA GGCAGTGCAT 
TCGGGGGGAT ATAAACCACA GAATGGCAGA GGTGTGGGGA CATTGCTGAT GCCATCAAAA 
GGTGTGGGTC ATGCAGTGAG GCCACAAAAC GGATATGCTG GATATCCCAT GAAGGGACCA 
GGTTATGGAG CCACAGCTGG GACGAACAAA GGCAGTGGAA TGAAAGGTTA CGGACAAACG 
GCTGGACAGA TGAGTGAACA AGGGGGTAGA CAGCTGGGAT ATGGTGTCAA AGCCGGCCCA 
GTCACTGGAC AGATGAACGG AGGCTTTGGT GCTCAACAAG CATTTGGTGG AGCAGGAGCC 
AAAGGCAATG GAAGAAATCC TGCAGGTTCG TTCAACAGAA ATGGAGTACA ACCTAATGTA 
GGATATGGCG TTTCAGCTGG GCCGTTATCT GGACCACAAA TCAAAGGTTT CAATCCTCAG 
GCTGGTGGAT ATGGAGCGAC GAAAGGAAAT GGTTATGAGG CAGCAGCTGC CTCACAGTAT 
ACACCTGGAA TAAGAGGCAA TGTATATGGA TCTAAAGCTG GCCCAAGCAT CAGACAACTG 
AAATCTGGTT ATGTTGCTCA AACTGGTGGT AATAGTGGGC AAGGAGAAAA ACCCAATGGG 
TATGGTGCTG TGGCTGGCCC AAGCACAGGA CAACAAATGA GAAATGGTTA CAATCTTCAA 
TCTGGAGGAC AAACGACTAA AGGCAATGGT CAAGGCGTGG TGGCTGGACC CTCTACGGCA 
AATGGAGCAT ATGGACAAGG AGGAATGGGA AGCAAACTTA TGAAGGGTTA TGGCCGAACA 
CCTTATGGAT CAGGTGTTCA GAGGGGAGTG TTGGGAGGCC TCGGAGTTCC TCGGCCTGCT 
AGGAACCAGC AGAAATCGTA TGGGAGCAGA ACTGGTGACA GAGGTCAGCC CAACAGCGGC 
TACAATGGCG GTTATGGCAG CACAGCTCTG GGACCTCGTT ATGGAAATGG CGGGATGAAA 
GGACCGGTAA AAGGCTATGC AAACTTACCA CATACTGGTA ATAGAGGAGC AGTTCTAAAC 
GGTTATGAAC AACAAGGCGG TGGAGTAGCT GCAAAACCAG CAAAATCAGG TGGTGGTAGT 
ATTCCTAATG GATATGGTGC CAGACCTAAC GGAAATCCTC ATTTAAATGG AGGTTCAGGG 
AGACCCCAGC CAAGTTTTGG AGCCACAGCT GAAGGCGGAG TTTTCAAAGG ATATGGCACT 
AAACCAAGTG GTCATGGTGG AAATGGTGCT GTTCTGAGTG GATATGGACG ACAGCCCAAC 
GGTTACAACC TGATGCCAAA TGGAAAGGGC CAGGCTGTTA GAGGAACAGA GGTATCAGGT 
GAAAGGAACC TCAAAGGTGG TGTGCACTTC CACCACCATC ATCATCAGCA GCAGCAGATG 
ACGGCACAGT TGGGGGATGT CACCCCACGA GCAGCTACCC CACCTATCCC GGCCCCTACA 
GGTGGTCTCC TTGTTATGGT GACCCAAGAG AAGTACCAAA AGCTGCCTTC ACCTTTGCCC 
CAGGGGAAAA AGTACAAACA CACAGCAATC CTGCCTGAGG AAAGCCCAGA ACCAGCACCT 
GCATATCCTG AAGCCAGAGG GCAGCACCTC TCACTGGAGC CTGCACCTAT TGGTCCAAAG 
GACAAGAATC AGAAGGGGGT CCATTCTGGA CCTGCAGTCG TGGTTCCCCA AAGCAAACCC 
GCACCAGAAC CAGCAGCTGT TCAAGGTTAT GTGACTGGAA ATGCTGTTCA TCTGCAAAAA 
GCTGCAGAAG CAGACGCTGT AGGTTTGGGG GGGGGCGCCC CAAAAACCAA AGCTGTTCCT 
GAAACAGCTG AGGAAAGGGG CGGTGTCCCT CCAAAGGAAA CACTGATCAC TGCGGGTGTG 
GGCGAGTCTG CAGCAGAGGA GGCTGACGCA CAACCTGACA GGCTGCAGGG CACAAGTATC 
CATGCTGGGG AGACGGGTAC TGAACCACAC CTAGGTTTGG AGAGTAGGGG ATCTTCTGCC 
TCAAAGGGAC CAGGAGCCAA ACTTGACTGT GGACTGAATG GTCAGTGGAT GAAAATACCA 
AGACCTGATT ACAATGCAGC AGCTGGTAGT TTGGATGGCT CACAAACTAA AGGTTATGGA 
GCAGGAGAAA ATGCTTTTCT AAACAATGGA TATGGAGCAG GGCTTGGGTA CCCGTATGCA 
GGGAAAGCAG CTCAAGCTGG ATATGGAAAA GGAGTTCACC CTGGGGCTGG ATATGAAAAT 
GGAAATCCAT ATGGAGGCTA TAGAAATGGG TATGGAGCAG GACCTCAGCC TGACTATTCA 
AGTTATGGTC AAGGTGTGCC GACTATTGAT GGCAAATCAG GTGGAAACCC AGGACTTCCT 
GCAGGACTAG ATGGAATGAG TCAGTTTGAG CCTCAGTCTG TTGGTCTGGG TCCAAATGGA 
AAAGGCGTCA TGTATGGTGT TGGGGGGCTG CAGTTTGGTG GACAACCTGG GAACTCCGGG 
ATAAATGGTG CAGGACAGTA TGGAGGTGTC GTTGCAAGTA TGGGAGGGAG TCTTGTGCCT 
GGAATGCACA GTTATGGTGG ATATGCTTAT AACCAAAATG CTCCTGGAAA ACAGGGTTAT 
GGAAGACTTC CTTATGGAGT TCAACAGGCT GGACTTAACC CTGAGGCTAA AGCCGCCGCC 
CAGTATGGTC ATACTGGGTC ATCATATCAG CCTGAATCTG TTGGCTTCGG ACAACCAGGG 
AAAACAGCAG GATCATACAG CGGGGGACAG GCTGCCTTTG CACCTCAGGC TCTTGGGTTT 
GGGGGTGATG ATAAATCTGG TAAATATGAT AAACAGGGAG CTTTCCAATC ACAGCGTCTC 
CGGCCTGAAG AAACAGCCGG TCAGACATAT GAGCGCCAGT CTGTTGGGTT GGATTCAGCA 
GGAAAGTCCT ATGTGAAAGG GGAAGTGCCA ACTGCAACTC TAGCAGCTGA AAGTGAGAGG 
ATTCCCATTA ATAAATATGA AAATGTGGGC TACATAAATG GACAAATGCA GCCAGAAGTT 
GCTGGGCTTC CTGCAGCTCC CACTGCCAGT TCCACCCTGG CCTTTTCCTC TCCTGATGGG 
ATTCCTGTAG AGGATGTTCA GGACATTCCT GATCCAGAGG GCACAGCCAG CTTCTTGGAG 
TCGGGACCCG TTACAGAAAT GCTGGGCGAG TCTCGGGGAC CGGAGGATGC CGAGAACCTG 
CCACGTCAGA TACACATCCA GCAGCATCTC AAACTCCATT TCAACCCACA AGGCAAAAAA 
AATGGCAAAT ATGATGCAAA TGGCTTCTTT GGGAACAATG GCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020567860.1
CDS	113..3460
Translation	MVRRCFHLTTVILWLVQAVHSGGYKPQNGRGVGTLLMPSKGVGHAVRPQNGYAGYPMKGPGYGATAGTNKGSGMKGYGQTAGQMSEQGGRQLGYGVKAGPVTGQMNGGFGAQQAFGGAGAKGNGRNPAGSFNRNGVQPNVGYGVSAGPLSGPQIKGFNPQAGGYGATKGNGYEAAAASQYTPGIRGNVYGSKAGPSIRQLKSGYVAQTGGNSGQGEKPNGYGAVAGPSTGQQMRNGYNLQSGGQTTKGNGQGVVAGPSTANGAYGQGGMGSKLMKGYGRTPYGSGVQRGVLGGLGVPRPARNQQKSYGSRTGDRGQPNSGYNGGYGSTALGPRYGNGGMKGPVKGYANLPHTGNRGAVLNGYEQQGGGVAAKPAKSGGGSIPNGYGARPNGNPHLNGGSGRPQPSFGATAEGGVFKGYGTKPSGHGGNGAVLSGYGRQPNGYNLMPNGKGQAVRGTEVSGERNLKGGVHFHHHHHQQQQMTAQLGDVTPRAATPPIPAPTGGLLVMVTQEKYQKLPSPLPQGKKYKHTAILPEESPEPAPAYPEARGQHLSLEPAPIGPKDKNQKGVHSGPAVVVPQSKPAPEPAAVQGYVTGNAVHLQKAAEADAVGLGGGAPKTKAVPETAEERGGVPPKETLITAGVGESAAEEADAQPDRLQGTSIHAGETGTEPHLGLESRGSSASKGPGAKLDCGLNGQWMKIPRPDYNAAAGSLDGSQTKGYGAGENAFLNNGYGAGLGYPYAGKAAQAGYGKGVHPGAGYENGNPYGGYRNGYGAGPQPDYSSYGQGVPTIDGKSGGNPGLPAGLDGMSQFEPQSVGLGPNGKGVMYGVGGLQFGGQPGNSGINGAGQYGGVVASMGGSLVPGMHSYGGYAYNQNAPGKQGYGRLPYGVQQAGLNPEAKAAAQYGHTGSSYQPESVGFGQPGKTAGSYSGGQAAFAPQALGFGGDDKSGKYDKQGAFQSQRLRPEETAGQTYERQSVGLDSAGKSYVKGEVPTATLAAESERIPINKYENVGYINGQMQPEVAGLPAAPTASSTLAFSSPDGIPVEDVQDIPDPEGTASFLESGPVTEMLGESRGPEDAENLPRQIHIQQHLKLHFNPQGKKNGKYDANGFFGNNGY

Target ORF information:

RefSeq Version	XM_020712201.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020712201.1

ORF Insert Sequence:

ATGGTGAGAC GATGCTTCCA TCTCACTACG GTGATCCTCT GGTTGGTGCA GGCAGTGCAT 
TCGGGGGGAT ATAAACCACA GAATGGCAGA GGTGTGGGGA CATTGCTGAT GCCATCAAAA 
GGTGTGGGTC ATGCAGTGAG GCCACAAAAC GGATATGCTG GATATCCCAT GAAGGGACCA 
GGTTATGGAG CCACAGCTGG GACGAACAAA GGCAGTGGAA TGAAAGGTTA CGGACAAACG 
GCTGGACAGA TGAGTGAACA AGGGGGTAGA CAGCTGGGAT ATGGTGTCAA AGCCGGCCCA 
GTCACTGGAC AGATGAACGG AGGCTTTGGT GCTCAACAAG CATTTGGTGG AGCAGGAGCC 
AAAGGCAATG GAAGAAATCC TGCAGGTTCG TTCAACAGAA ATGGAGTACA ACCTAATGTA 
GGATATGGCG TTTCAGCTGG GCCGTTATCT GGACCACAAA TCAAAGGTTT CAATCCTCAG 
GCTGGTGGAT ATGGAGCGAC GAAAGGAAAT GGTTATGAGG CAGCAGCTGC CTCACAGTAT 
ACACCTGGAA TAAGAGGCAA TGTATATGGA TCTAAAGCTG GCCCAAGCAT CAGACAACTG 
AAATCTGGTT ATGTTGCTCA AACTGGTGGT AATAGTGGGC AAGGAGAAAA ACCCAATGGG 
TATGGTGCTG TGGCTGGCCC AAGCACAGGA CAACAAATGA GAAATGGTTA CAATCTTCAA 
TCTGGAGGAC AAACGACTAA AGGCAATGGT CAAGGCGTGG TGGCTGGACC CTCTACGGCA 
AATGGAGCAT ATGGACAAGG AGGAATGGGA AGCAAACTTA TGAAGGGTTA TGGCCGAACA 
CCTTATGGAT CAGGTGTTCA GAGGGGAGTG TTGGGAGGCC TCGGAGTTCC TCGGCCTGCT 
AGGAACCAGC AGAAATCGTA TGGGAGCAGA ACTGGTGACA GAGGTCAGCC CAACAGCGGC 
TACAATGGCG GTTATGGCAG CACAGCTCTG GGACCTCGTT ATGGAAATGG CGGGATGAAA 
GGACCGGTAA AAGGCTATGC AAACTTACCA CATACTGGTA ATAGAGGAGC AGTTCTAAAC 
GGTTATGAAC AACAAGGCGG TGGAGTAGCT GCAAAACCAG CAAAATCAGG TGGTGGTAGT 
ATTCCTAATG GATATGGTGC CAGACCTAAC GGAAATCCTC ATTTAAATGG AGGTTCAGGG 
AGACCCCAGC CAAGTTTTGG AGCCACAGCT GAAGGCGGAG TTTTCAAAGG ATATGGCACT 
AAACCAAGTG GTCATGGTGG AAATGGTGCT GTTCTGAGTG GATATGGACG ACAGCCCAAC 
GGTTACAACC TGATGCCAAA TGGAAAGGGC CAGGCTGTTA GAGGAACAGA GGTATCAGGT 
GAAAGGAACC TCAAAGGTGG TGTGCACTTC CACCACCATC ATCATCAGCA GCAGCAGATG 
ACGGCACAGT TGGGGGATGT CACCCCACGA GCAGCTACCC CACCTATCCC GGCCCCTACA 
GGTGGTCTCC TTGTTATGGT GACCCAAGAG AAGTACCAAA AGCTGCCTTC ACCTTTGCCC 
CAGGGGAAAA AGTACAAACA CACAGCAATC CTGCCTGAGG AAAGCCCAGA ACCAGCACCT 
GCATATCCTG AAGCCAGAGG GCAGCACCTC TCACTGGAGC CTGCACCTAT TGGTCCAAAG 
GACAAGAATC AGAAGGGGGT CCATTCTGGA CCTGCAGTCG TGGTTCCCCA AAGCAAACCC 
GCACCAGAAC CAGCAGCTGT TCAAGGTTAT GTGACTGGAA ATGCTGTTCA TCTGCAAAAA 
GCTGCAGAAG CAGACGCTGT AGGTTTGGGG GGGGGCGCCC CAAAAACCAA AGCTGTTCCT 
GAAACAGCTG AGGAAAGGGG CGGTGTCCCT CCAAAGGAAA CACTGATCAC TGCGGGTGTG 
GGCGAGTCTG CAGCAGAGGA GGCTGACGCA CAACCTGACA GGCTGCAGGG CACAAGTATC 
CATGCTGGGG AGACGGGTAC TGAACCACAC CTAGGTTTGG AGAGTAGGGG ATCTTCTGCC 
TCAAAGGGAC CAGGAGCCAA ACTTGACTGT GGACTGAATG GTCAGTGGAT GAAAATACCA 
AGACCTGATT ACAATGCAGC AGCTGGTAGT TTGGATGGCT CACAAACTAA AGGTTATGGA 
GCAGGAGAAA ATGCTTTTCT AAACAATGGA TATGGAGCAG GGCTTGGGTA CCCGTATGCA 
GGGAAAGCAG CTCAAGCTGG ATATGGAAAA GGAGTTCACC CTGGGGCTGG ATATGAAAAT 
GGAAATCCAT ATGGAGGCTA TAGAAATGGG TATGGAGCAG GACCTCAGCC TGACTATTCA 
AGTTATGGTC AAGGTGTGCC GACTATTGAT GGCAAATCAG GTGGAAACCC AGGACTTCCT 
GCAGGACTAG ATGGAATGAG TCAGTTTGAG CCTCAGTCTG TTGGTCTGGG TCCAAATGGA 
AAAGGCGTCA TGTATGGTGT TGGGGGGCTG CAGTTTGGTG GACAACCTGG GAACTCCGGG 
ATAAATGGTG CAGGACAGTA TGGAGGTGTC GTTGCAAGTA TGGGAGGGAG TCTTGTGCCT 
GGAATGCACA GTTATGGTGG ATATGCTTAT AACCAAAATG CTCCTGGAAA ACAGGGTTAT 
GGAAGACTTC CTTATGGAGT TCAACAGGCT GGACTTAACC CTGAGGCTAA AGCCGCCGCC 
CAGTATGGTC ATACTGGGTC ATCATATCAG CCTGAATCTG TTGGCTTCGG ACAACCAGGG 
AAAACAGCAG GATCATACAG CGGGGGACAG GCTGCCTTTG CACCTCAGGC TCTTGGGTTT 
GGGGGTGATG ATAAATCTGG TAAATATGAT AAACAGGGAG CTTTCCAATC ACAGCGTCTC 
CGGCCTGAAG AAACAGCCGG TCAGACATAT GAGCGCCAGT CTGTTGGGTT GGATTCAGCA 
GGAAAGTCCT ATGTGAAAGG GGAAGTGCCA ACTGCAACTC TAGCAGCTGA AAGTGAGAGG 
ATTCCCATTA ATAAATATGA AAATGTGGGC TACATAAATG GACAAATGCA GCCAGAAGTT 
GCTGGGCTTC CTGCAGCTCC CACTGCCAGT TCCACCCTGG CCTTTTCCTC TCCTGATGGG 
ATTCCTGTAG AGGATGTTCA GGACATTCCT GATCCAGAGG GCACAGCCAG CTTCTTGGAG 
TCGGGACCCG TTACAGAAAT GCTGGGCGAG TCTCGGGGAC CGGAGGATGC CGAGAACCTG 
CCACGTCAGA TACACATCCA GCAGCATCTC AAACTCCATT TCAACCCACA AGGCAAAAAA 
AATGGCAAAT ATGATGCAAA TGGCTTCTTT GGGAACAATG GCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101174706 ( XM_020712202.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020567861.1 Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OJa74385
Clone ID Related Accession (Same CDS sequence)	XM_020712202.1
Accession Version	XM_020712202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	fibroin heavy chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019877.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGAC GATGCTTCCA TCTCACTACG GTGATCCTCT GGTTGGTGCA GGCAGTGCAT 
TCGGGGGGAT ATAAACCACA GAATGGCAGA GGTGTGGGGA CATTGCTGAT GCCATCAAAA 
GGTGTGGGTC ATGCAGTGAG GCCACAAAAC GGATATGCTG GATATCCCAT GAAGGGACCA 
GGTTATGGAG CCACAGCTGG GACGAACAAA GGCAGTGGAA TGAAAGGTTA CGGACAAACG 
GCTGGACAGA TGAGTGAACA AGGGGGTAGA CAGCTGGGAT ATGGTGTCAA AGCCGGCCCA 
GTCACTGGAC AGATGAACGG AGGCTTTGGT GCTCAACAAG CATTTGGTGG AGCAGGAGCC 
AAAGGCAATG GAAGAAATCC TGCAGGTTCG TTCAACAGAA ATGGAGTACA ACCTAATGTA 
GGATATGGCG TTTCAGCTGG GCCGTTATCT GGACCACAAA TCAAAGGTTT CAATCCTCAG 
GCTGGTGGAT ATGGAGCGAC GAAAGGAAAT GGTTATGAGG CAGCAGCTGC CTCACAGTAT 
ACACCTGGAA TAAGAGGCAA TGTATATGGA TCTAAAGCTG GCCCAAGCAT CAGACAACTG 
AAATCTGGTT ATGTTGCTCA AACTGGTGGT AATAGTGGGC AAGGAGAAAA ACCCAATGGG 
TATGGTGCTG TGGCTGGCCC AAGCACAGGA CAACAAATGA GAAATGGTTA CAATCTTCAA 
TCTGGAGGAC AAACGACTAA AGGCAATGGT CAAGGCGTGG TGGCTGGACC CTCTACGGCA 
AATGGAGCAT ATGGACAAGG AGGAATGGGA AGCAAACTTA TGAAGGGTTA TGGCCGAACA 
CCTTATGGAT CAGGTGTTCA GAGGGGAGTG TTGGGAGGCC TCGGAGTTCC TCGGCCTGCT 
AGGAACCAGC AGAAATCGTA TGGGAGCAGA ACTGGTGACA GAGGTCAGCC CAACAGCGGC 
TACAATGGCG GTTATGGCAG CACAGCTCTG GGACCTCGTT ATGGAAATGG CGGGATGAAA 
GGACCGGTAA AAGGTTATGA ACAACAAGGC GGTGGAGTAG CTGCAAAACC AGCAAAATCA 
GGTGGTGGTA GTATTCCTAA TGGATATGGT GCCAGACCTA ACGGAAATCC TCATTTAAAT 
GGAGGTTCAG GGAGACCCCA GCCAAGTTTT GGAGCCACAG CTGAAGGCGG AGTTTTCAAA 
GGATATGGCA CTAAACCAAG TGGTCATGGT GGAAATGGTG CTGTTCTGAG TGGATATGGA 
CGACAGCCCA ACGGTTACAA CCTGATGCCA AATGGAAAGG GCCAGGCTGT TAGAGGAACA 
GAGGTATCAG GTGAAAGGAA CCTCAAAGGT GGTGTGCACT TCCACCACCA TCATCATCAG 
CAGCAGCAGA TGACGGCACA GTTGGGGGAT GTCACCCCAC GAGCAGCTAC CCCACCTATC 
CCGGCCCCTA CAGGTGGTCT CCTTGTTATG GTGACCCAAG AGAAGTACCA AAAGCTGCCT 
TCACCTTTGC CCCAGGGGAA AAAGTACAAA CACACAGCAA TCCTGCCTGA GGAAAGCCCA 
GAACCAGCAC CTGCATATCC TGAAGCCAGA GGGCAGCACC TCTCACTGGA GCCTGCACCT 
ATTGGTCCAA AGGACAAGAA TCAGAAGGGG GTCCATTCTG GACCTGCAGT CGTGGTTCCC 
CAAAGCAAAC CCGCACCAGA ACCAGCAGCT GTTCAAGGTT ATGTGACTGG AAATGCTGTT 
CATCTGCAAA AAGCTGCAGA AGCAGACGCT GTAGGTTTGG GGGGGGGCGC CCCAAAAACC 
AAAGCTGTTC CTGAAACAGC TGAGGAAAGG GGCGGTGTCC CTCCAAAGGA AACACTGATC 
ACTGCGGGTG TGGGCGAGTC TGCAGCAGAG GAGGCTGACG CACAACCTGA CAGGCTGCAG 
GGCACAAGTA TCCATGCTGG GGAGACGGGT ACTGAACCAC ACCTAGGTTT GGAGAGTAGG 
GGATCTTCTG CCTCAAAGGG ACCAGGAGCC AAACTTGACT GTGGACTGAA TGGTCAGTGG 
ATGAAAATAC CAAGACCTGA TTACAATGCA GCAGCTGGTA GTTTGGATGG CTCACAAACT 
AAAGGTTATG GAGCAGGAGA AAATGCTTTT CTAAACAATG GATATGGAGC AGGGCTTGGG 
TACCCGTATG CAGGGAAAGC AGCTCAAGCT GGATATGGAA AAGGAGTTCA CCCTGGGGCT 
GGATATGAAA ATGGAAATCC ATATGGAGCT CTCAGTGAAG CAGGCTATAG AAATGGGTAT 
GGAGCAGGAC CTCAGCCTGA CTATTCAAGT TATGGTCAAG GTGTGCCGAC TATTGATGGC 
AAATCAGGTG GAAACCCAGG ACTTCCTGCA GGACTAGATG GAATGAGTCA GTTTGAGCCT 
CAGTCTGTTG GTCTGGGTCC AAATGGAAAA GGCGTCATGT ATGGTGTTGG GGGGCTGCAG 
TTTGGTGGAC AACCTGGGAA CTCCGGGATA AATGGTGCAG GACAGTATGG AGGTGTCGTT 
GCAAGTATGG GAGGGAGTCT TGTGCCTGGA ATGCACAGTT ATGGTGGATA TGCTTATAAC 
CAAAATGCTC CTGGAAAACA GGGTTATGGA AGACTTCCTT ATGGAGTTCA ACAGGCTGGA 
CTTAACCCTG AGGCTAAAGC CGCCGCCCAG TATGGTCATA CTGGGTCATC ATATCAGCCT 
GAATCTGTTG GCTTCGGACA ACCAGGGAAA ACAGCAGGAT CATACAGCGG GGGACAGGCT 
GCCTTTGCAC CTCAGGCTCT TGGGTTTGGG GGTGATGATA AATCTGGTAA ATATGATAAA 
CAGGGAGCTT TCCAATCACA GCGTCTCCGG CCTGAAGAAA CAGCCGGTCA GACATATGAG 
CGCCAGTCTG TTGGGTTGGA TTCAGCAGGA AAGTCCTATG TGAAAGGGGA AGTGCCAACT 
GCAACTCTAG CAGCTGAAAG TGAGAGGATT CCCATTAATA AATATGAAAA TGTGGGCTAC 
ATAAATGGAC AAATGCAGCC AGAAGTTGCT GGGCTTCCTG CAGCTCCCAC TGCCAGTTCC 
ACCCTGGCCT TTTCCTCTCC TGATGGGATT CCTGTAGAGG ATGTTCAGGA CATTCCTGAT 
CCAGAGGGCA CAGCCAGCTT CTTGGAGTCG GGACCCGTTA CAGAAATGCT GGGCGAGTCT 
CGGGGACCGG AGGATGCCGA GAACCTGCCA CGTCAGATAC ACATCCAGCA GCATCTCAAA 
CTCCATTTCA ACCCACAAGG CAAAAAAAAT GGCAAATATG ATGCAAATGG CTTCTTTGGG 
AACAATGGCT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020567861.1
CDS	113..3427
Translation	MVRRCFHLTTVILWLVQAVHSGGYKPQNGRGVGTLLMPSKGVGHAVRPQNGYAGYPMKGPGYGATAGTNKGSGMKGYGQTAGQMSEQGGRQLGYGVKAGPVTGQMNGGFGAQQAFGGAGAKGNGRNPAGSFNRNGVQPNVGYGVSAGPLSGPQIKGFNPQAGGYGATKGNGYEAAAASQYTPGIRGNVYGSKAGPSIRQLKSGYVAQTGGNSGQGEKPNGYGAVAGPSTGQQMRNGYNLQSGGQTTKGNGQGVVAGPSTANGAYGQGGMGSKLMKGYGRTPYGSGVQRGVLGGLGVPRPARNQQKSYGSRTGDRGQPNSGYNGGYGSTALGPRYGNGGMKGPVKGYEQQGGGVAAKPAKSGGGSIPNGYGARPNGNPHLNGGSGRPQPSFGATAEGGVFKGYGTKPSGHGGNGAVLSGYGRQPNGYNLMPNGKGQAVRGTEVSGERNLKGGVHFHHHHHQQQQMTAQLGDVTPRAATPPIPAPTGGLLVMVTQEKYQKLPSPLPQGKKYKHTAILPEESPEPAPAYPEARGQHLSLEPAPIGPKDKNQKGVHSGPAVVVPQSKPAPEPAAVQGYVTGNAVHLQKAAEADAVGLGGGAPKTKAVPETAEERGGVPPKETLITAGVGESAAEEADAQPDRLQGTSIHAGETGTEPHLGLESRGSSASKGPGAKLDCGLNGQWMKIPRPDYNAAAGSLDGSQTKGYGAGENAFLNNGYGAGLGYPYAGKAAQAGYGKGVHPGAGYENGNPYGALSEAGYRNGYGAGPQPDYSSYGQGVPTIDGKSGGNPGLPAGLDGMSQFEPQSVGLGPNGKGVMYGVGGLQFGGQPGNSGINGAGQYGGVVASMGGSLVPGMHSYGGYAYNQNAPGKQGYGRLPYGVQQAGLNPEAKAAAQYGHTGSSYQPESVGFGQPGKTAGSYSGGQAAFAPQALGFGGDDKSGKYDKQGAFQSQRLRPEETAGQTYERQSVGLDSAGKSYVKGEVPTATLAAESERIPINKYENVGYINGQMQPEVAGLPAAPTASSTLAFSSPDGIPVEDVQDIPDPEGTASFLESGPVTEMLGESRGPEDAENLPRQIHIQQHLKLHFNPQGKKNGKYDANGFFGNNGY

Target ORF information:

RefSeq Version	XM_020712202.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020712202.1

ORF Insert Sequence:

ATGGTGAGAC GATGCTTCCA TCTCACTACG GTGATCCTCT GGTTGGTGCA GGCAGTGCAT 
TCGGGGGGAT ATAAACCACA GAATGGCAGA GGTGTGGGGA CATTGCTGAT GCCATCAAAA 
GGTGTGGGTC ATGCAGTGAG GCCACAAAAC GGATATGCTG GATATCCCAT GAAGGGACCA 
GGTTATGGAG CCACAGCTGG GACGAACAAA GGCAGTGGAA TGAAAGGTTA CGGACAAACG 
GCTGGACAGA TGAGTGAACA AGGGGGTAGA CAGCTGGGAT ATGGTGTCAA AGCCGGCCCA 
GTCACTGGAC AGATGAACGG AGGCTTTGGT GCTCAACAAG CATTTGGTGG AGCAGGAGCC 
AAAGGCAATG GAAGAAATCC TGCAGGTTCG TTCAACAGAA ATGGAGTACA ACCTAATGTA 
GGATATGGCG TTTCAGCTGG GCCGTTATCT GGACCACAAA TCAAAGGTTT CAATCCTCAG 
GCTGGTGGAT ATGGAGCGAC GAAAGGAAAT GGTTATGAGG CAGCAGCTGC CTCACAGTAT 
ACACCTGGAA TAAGAGGCAA TGTATATGGA TCTAAAGCTG GCCCAAGCAT CAGACAACTG 
AAATCTGGTT ATGTTGCTCA AACTGGTGGT AATAGTGGGC AAGGAGAAAA ACCCAATGGG 
TATGGTGCTG TGGCTGGCCC AAGCACAGGA CAACAAATGA GAAATGGTTA CAATCTTCAA 
TCTGGAGGAC AAACGACTAA AGGCAATGGT CAAGGCGTGG TGGCTGGACC CTCTACGGCA 
AATGGAGCAT ATGGACAAGG AGGAATGGGA AGCAAACTTA TGAAGGGTTA TGGCCGAACA 
CCTTATGGAT CAGGTGTTCA GAGGGGAGTG TTGGGAGGCC TCGGAGTTCC TCGGCCTGCT 
AGGAACCAGC AGAAATCGTA TGGGAGCAGA ACTGGTGACA GAGGTCAGCC CAACAGCGGC 
TACAATGGCG GTTATGGCAG CACAGCTCTG GGACCTCGTT ATGGAAATGG CGGGATGAAA 
GGACCGGTAA AAGGTTATGA ACAACAAGGC GGTGGAGTAG CTGCAAAACC AGCAAAATCA 
GGTGGTGGTA GTATTCCTAA TGGATATGGT GCCAGACCTA ACGGAAATCC TCATTTAAAT 
GGAGGTTCAG GGAGACCCCA GCCAAGTTTT GGAGCCACAG CTGAAGGCGG AGTTTTCAAA 
GGATATGGCA CTAAACCAAG TGGTCATGGT GGAAATGGTG CTGTTCTGAG TGGATATGGA 
CGACAGCCCA ACGGTTACAA CCTGATGCCA AATGGAAAGG GCCAGGCTGT TAGAGGAACA 
GAGGTATCAG GTGAAAGGAA CCTCAAAGGT GGTGTGCACT TCCACCACCA TCATCATCAG 
CAGCAGCAGA TGACGGCACA GTTGGGGGAT GTCACCCCAC GAGCAGCTAC CCCACCTATC 
CCGGCCCCTA CAGGTGGTCT CCTTGTTATG GTGACCCAAG AGAAGTACCA AAAGCTGCCT 
TCACCTTTGC CCCAGGGGAA AAAGTACAAA CACACAGCAA TCCTGCCTGA GGAAAGCCCA 
GAACCAGCAC CTGCATATCC TGAAGCCAGA GGGCAGCACC TCTCACTGGA GCCTGCACCT 
ATTGGTCCAA AGGACAAGAA TCAGAAGGGG GTCCATTCTG GACCTGCAGT CGTGGTTCCC 
CAAAGCAAAC CCGCACCAGA ACCAGCAGCT GTTCAAGGTT ATGTGACTGG AAATGCTGTT 
CATCTGCAAA AAGCTGCAGA AGCAGACGCT GTAGGTTTGG GGGGGGGCGC CCCAAAAACC 
AAAGCTGTTC CTGAAACAGC TGAGGAAAGG GGCGGTGTCC CTCCAAAGGA AACACTGATC 
ACTGCGGGTG TGGGCGAGTC TGCAGCAGAG GAGGCTGACG CACAACCTGA CAGGCTGCAG 
GGCACAAGTA TCCATGCTGG GGAGACGGGT ACTGAACCAC ACCTAGGTTT GGAGAGTAGG 
GGATCTTCTG CCTCAAAGGG ACCAGGAGCC AAACTTGACT GTGGACTGAA TGGTCAGTGG 
ATGAAAATAC CAAGACCTGA TTACAATGCA GCAGCTGGTA GTTTGGATGG CTCACAAACT 
AAAGGTTATG GAGCAGGAGA AAATGCTTTT CTAAACAATG GATATGGAGC AGGGCTTGGG 
TACCCGTATG CAGGGAAAGC AGCTCAAGCT GGATATGGAA AAGGAGTTCA CCCTGGGGCT 
GGATATGAAA ATGGAAATCC ATATGGAGCT CTCAGTGAAG CAGGCTATAG AAATGGGTAT 
GGAGCAGGAC CTCAGCCTGA CTATTCAAGT TATGGTCAAG GTGTGCCGAC TATTGATGGC 
AAATCAGGTG GAAACCCAGG ACTTCCTGCA GGACTAGATG GAATGAGTCA GTTTGAGCCT 
CAGTCTGTTG GTCTGGGTCC AAATGGAAAA GGCGTCATGT ATGGTGTTGG GGGGCTGCAG 
TTTGGTGGAC AACCTGGGAA CTCCGGGATA AATGGTGCAG GACAGTATGG AGGTGTCGTT 
GCAAGTATGG GAGGGAGTCT TGTGCCTGGA ATGCACAGTT ATGGTGGATA TGCTTATAAC 
CAAAATGCTC CTGGAAAACA GGGTTATGGA AGACTTCCTT ATGGAGTTCA ACAGGCTGGA 
CTTAACCCTG AGGCTAAAGC CGCCGCCCAG TATGGTCATA CTGGGTCATC ATATCAGCCT 
GAATCTGTTG GCTTCGGACA ACCAGGGAAA ACAGCAGGAT CATACAGCGG GGGACAGGCT 
GCCTTTGCAC CTCAGGCTCT TGGGTTTGGG GGTGATGATA AATCTGGTAA ATATGATAAA 
CAGGGAGCTT TCCAATCACA GCGTCTCCGG CCTGAAGAAA CAGCCGGTCA GACATATGAG 
CGCCAGTCTG TTGGGTTGGA TTCAGCAGGA AAGTCCTATG TGAAAGGGGA AGTGCCAACT 
GCAACTCTAG CAGCTGAAAG TGAGAGGATT CCCATTAATA AATATGAAAA TGTGGGCTAC 
ATAAATGGAC AAATGCAGCC AGAAGTTGCT GGGCTTCCTG CAGCTCCCAC TGCCAGTTCC 
ACCCTGGCCT TTTCCTCTCC TGATGGGATT CCTGTAGAGG ATGTTCAGGA CATTCCTGAT 
CCAGAGGGCA CAGCCAGCTT CTTGGAGTCG GGACCCGTTA CAGAAATGCT GGGCGAGTCT 
CGGGGACCGG AGGATGCCGA GAACCTGCCA CGTCAGATAC ACATCCAGCA GCATCTCAAA 
CTCCATTTCA ACCCACAAGG CAAAAAAAAT GGCAAATATG ATGCAAATGG CTTCTTTGGG 
AACAATGGCT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101174706 ( XM_020712203.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_020567862.1 Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OJa74386
Clone ID Related Accession (Same CDS sequence)	XM_020712203.1
Accession Version	XM_020712203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	fibroin heavy chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019877.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGAC GATGCTTCCA TCTCACTACG GTGATCCTCT GGTTGGTGCA GGCAGTGCAT 
TCGGGGGGAT ATAAACCACA GAATGGCAGA GGTGTGGGGA CATTGCTGAT GCCATCAAAA 
GGTGTGGGTC ATGCAGTGAG GCCACAAAAC GGATATGCTG GATATCCCAT GAAGGGACCA 
GGTTATGGAG CCACAGCTGG GACGAACAAA GGCAGTGGAA TGAAAGGTTA CGGACAAACG 
GCTGGACAGA TGAGTGAACA AGGGGGTAGA CAGCTGGGAT ATGGTGTCAA AGCCGGCCCA 
GTCACTGGAC AGATGAACGG AGGCTTTGGT GCTCAACAAG CATTTGGTGG AGCAGGAGCC 
AAAGGCAATG GAAGAAATCC TGCAGGTTCG TTCAACAGAA ATGGAGTACA ACCTAATGTA 
GGATATGGCG TTTCAGCTGG GCCGTTATCT GGACCACAAA TCAAAGGTTT CAATCCTCAG 
GCTGGTGGAT ATGGAGCGAC GAAAGGAAAT GGTTATGAGG CAGCAGCTGC CTCACAGTAT 
ACACCTGGAA TAAGAGGCAA TGTATATGGA TCTAAAGCTG GCCCAAGCAT CAGACAACTG 
AAATCTGGTT ATGTTGCTCA AACTGGTGGT AATAGTGGGC AAGGAGAAAA ACCCAATGGG 
TATGGTGCTG TGGCTGGCCC AAGCACAGGA CAACAAATGA GAAATGGTTA CAATCTTCAA 
TCTGGAGGAC AAACGACTAA AGGCAATGGT CAAGGCGTGG TGGCTGGACC CTCTACGGCA 
AATGGAGCAT ATGGACAAGG AGGAATGGGA AGCAAACTTA TGAAGGGTTA TGGCCGAACA 
CCTTATGGAT CAGGTGTTCA GAGGGGAGTG TTGGGAGGCC TCGGAGTTCC TCGGCCTGCT 
AGGAACCAGC AGAAATCGTA TGGGAGCAGA ACTGGTGACA GAGGTCAGCC CAACAGCGGC 
TACAATGGCG GTTATGGCAG CACAGCTCTG GGACCTCGTT ATGGAAATGG CGGGATGAAA 
GGACCGGTAA AAGGCTATGC AAACTTACCA CATACTGGTA ATAGAGGAGC AGTTCTAAAC 
GGTTATGAAC AACAAGGCGG TGGAGTAGCT GCAAAACCAG CAAAATCAGG TTTTGGAGCC 
ACAGCTGAAG GCGGAGTTTT CAAAGGATAT GGCACTAAAC CAAGTGGTCA TGGTGGAAAT 
GGTGCTGTTC TGAGTGGATA TGGACGACAG CCCAACGGTT ACAACCTGAT GCCAAATGGA 
AAGGGCCAGG CTGTTAGAGG AACAGAGGTA TCAGGTGAAA GGAACCTCAA AGGTGGTGTG 
CACTTCCACC ACCATCATCA TCAGCAGCAG CAGATGACGG CACAGTTGGG GGATGTCACC 
CCACGAGCAG CTACCCCACC TATCCCGGCC CCTACAGGTG GTCTCCTTGT TATGGTGACC 
CAAGAGAAGT ACCAAAAGCT GCCTTCACCT TTGCCCCAGG GGAAAAAGTA CAAACACACA 
GCAATCCTGC CTGAGGAAAG CCCAGAACCA GCACCTGCAT ATCCTGAAGC CAGAGGGCAG 
CACCTCTCAC TGGAGCCTGC ACCTATTGGT CCAAAGGACA AGAATCAGAA GGGGGTCCAT 
TCTGGACCTG CAGTCGTGGT TCCCCAAAGC AAACCCGCAC CAGAACCAGC AGCTGTTCAA 
GGTTATGTGA CTGGAAATGC TGTTCATCTG CAAAAAGCTG CAGAAGCAGA CGCTGTAGGT 
TTGGGGGGGG GCGCCCCAAA AACCAAAGCT GTTCCTGAAA CAGCTGAGGA AAGGGGCGGT 
GTCCCTCCAA AGGAAACACT GATCACTGCG GGTGTGGGCG AGTCTGCAGC AGAGGAGGCT 
GACGCACAAC CTGACAGGCT GCAGGGCACA AGTATCCATG CTGGGGAGAC GGGTACTGAA 
CCACACCTAG GTTTGGAGAG TAGGGGATCT TCTGCCTCAA AGGGACCAGG AGCCAAACTT 
GACTGTGGAC TGAATGGTCA GTGGATGAAA ATACCAAGAC CTGATTACAA TGCAGCAGCT 
GGTAGTTTGG ATGGCTCACA AACTAAAGGT TATGGAGCAG GAGAAAATGC TTTTCTAAAC 
AATGGATATG GAGCAGGGCT TGGGTACCCG TATGCAGGGA AAGCAGCTCA AGCTGGATAT 
GGAAAAGGAG TTCACCCTGG GGCTGGATAT GAAAATGGAA ATCCATATGG AGCTCTCAGT 
GAAGCAGGCT ATAGAAATGG GTATGGAGCA GGACCTCAGC CTGACTATTC AAGTTATGGT 
CAAGGTGTGC CGACTATTGA TGGCAAATCA GGTGGAAACC CAGGACTTCC TGCAGGACTA 
GATGGAATGA GTCAGTTTGA GCCTCAGTCT GTTGGTCTGG GTCCAAATGG AAAAGGCGTC 
ATGTATGGTG TTGGGGGGCT GCAGTTTGGT GGACAACCTG GGAACTCCGG GATAAATGGT 
GCAGGACAGT ATGGAGGTGT CGTTGCAAGT ATGGGAGGGA GTCTTGTGCC TGGAATGCAC 
AGTTATGGTG GATATGCTTA TAACCAAAAT GCTCCTGGAA AACAGGGTTA TGGAAGACTT 
CCTTATGGAG TTCAACAGGC TGGACTTAAC CCTGAGGCTA AAGCCGCCGC CCAGTATGGT 
CATACTGGGT CATCATATCA GCCTGAATCT GTTGGCTTCG GACAACCAGG GAAAACAGCA 
GGATCATACA GCGGGGGACA GGCTGCCTTT GCACCTCAGG CTCTTGGGTT TGGGGGTGAT 
GATAAATCTG GTAAATATGA TAAACAGGGA GCTTTCCAAT CACAGCGTCT CCGGCCTGAA 
GAAACAGCCG GTCAGACATA TGAGCGCCAG TCTGTTGGGT TGGATTCAGC AGGAAAGTCC 
TATGTGAAAG GGGAAGTGCC AACTGCAACT CTAGCAGCTG AAAGTGAGAG GATTCCCATT 
AATAAATATG AAAATGTGGG CTACATAAAT GGACAAATGC AGCCAGAAGT TGCTGGGCTT 
CCTGCAGCTC CCACTGCCAG TTCCACCCTG GCCTTTTCCT CTCCTGATGG GATTCCTGTA 
GAGGATGTTC AGGACATTCC TGATCCAGAG GGCACAGCCA GCTTCTTGGA GTCGGGACCC 
GTTACAGAAA TGCTGGGCGA GTCTCGGGGA CCGGAGGATG CCGAGAACCT GCCACGTCAG 
ATACACATCC AGCAGCATCT CAAACTCCAT TTCAACCCAC AAGGCAAAAA AAATGGCAAA 
TATGATGCAA ATGGCTTCTT TGGGAACAAT GGCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020567862.1
CDS	113..3391
Translation	MVRRCFHLTTVILWLVQAVHSGGYKPQNGRGVGTLLMPSKGVGHAVRPQNGYAGYPMKGPGYGATAGTNKGSGMKGYGQTAGQMSEQGGRQLGYGVKAGPVTGQMNGGFGAQQAFGGAGAKGNGRNPAGSFNRNGVQPNVGYGVSAGPLSGPQIKGFNPQAGGYGATKGNGYEAAAASQYTPGIRGNVYGSKAGPSIRQLKSGYVAQTGGNSGQGEKPNGYGAVAGPSTGQQMRNGYNLQSGGQTTKGNGQGVVAGPSTANGAYGQGGMGSKLMKGYGRTPYGSGVQRGVLGGLGVPRPARNQQKSYGSRTGDRGQPNSGYNGGYGSTALGPRYGNGGMKGPVKGYANLPHTGNRGAVLNGYEQQGGGVAAKPAKSGFGATAEGGVFKGYGTKPSGHGGNGAVLSGYGRQPNGYNLMPNGKGQAVRGTEVSGERNLKGGVHFHHHHHQQQQMTAQLGDVTPRAATPPIPAPTGGLLVMVTQEKYQKLPSPLPQGKKYKHTAILPEESPEPAPAYPEARGQHLSLEPAPIGPKDKNQKGVHSGPAVVVPQSKPAPEPAAVQGYVTGNAVHLQKAAEADAVGLGGGAPKTKAVPETAEERGGVPPKETLITAGVGESAAEEADAQPDRLQGTSIHAGETGTEPHLGLESRGSSASKGPGAKLDCGLNGQWMKIPRPDYNAAAGSLDGSQTKGYGAGENAFLNNGYGAGLGYPYAGKAAQAGYGKGVHPGAGYENGNPYGALSEAGYRNGYGAGPQPDYSSYGQGVPTIDGKSGGNPGLPAGLDGMSQFEPQSVGLGPNGKGVMYGVGGLQFGGQPGNSGINGAGQYGGVVASMGGSLVPGMHSYGGYAYNQNAPGKQGYGRLPYGVQQAGLNPEAKAAAQYGHTGSSYQPESVGFGQPGKTAGSYSGGQAAFAPQALGFGGDDKSGKYDKQGAFQSQRLRPEETAGQTYERQSVGLDSAGKSYVKGEVPTATLAAESERIPINKYENVGYINGQMQPEVAGLPAAPTASSTLAFSSPDGIPVEDVQDIPDPEGTASFLESGPVTEMLGESRGPEDAENLPRQIHIQQHLKLHFNPQGKKNGKYDANGFFGNNGY

Target ORF information:

RefSeq Version	XM_020712203.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes fibroin heavy chain (LOC101174706), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020712203.1

ORF Insert Sequence:

ATGGTGAGAC GATGCTTCCA TCTCACTACG GTGATCCTCT GGTTGGTGCA GGCAGTGCAT 
TCGGGGGGAT ATAAACCACA GAATGGCAGA GGTGTGGGGA CATTGCTGAT GCCATCAAAA 
GGTGTGGGTC ATGCAGTGAG GCCACAAAAC GGATATGCTG GATATCCCAT GAAGGGACCA 
GGTTATGGAG CCACAGCTGG GACGAACAAA GGCAGTGGAA TGAAAGGTTA CGGACAAACG 
GCTGGACAGA TGAGTGAACA AGGGGGTAGA CAGCTGGGAT ATGGTGTCAA AGCCGGCCCA 
GTCACTGGAC AGATGAACGG AGGCTTTGGT GCTCAACAAG CATTTGGTGG AGCAGGAGCC 
AAAGGCAATG GAAGAAATCC TGCAGGTTCG TTCAACAGAA ATGGAGTACA ACCTAATGTA 
GGATATGGCG TTTCAGCTGG GCCGTTATCT GGACCACAAA TCAAAGGTTT CAATCCTCAG 
GCTGGTGGAT ATGGAGCGAC GAAAGGAAAT GGTTATGAGG CAGCAGCTGC CTCACAGTAT 
ACACCTGGAA TAAGAGGCAA TGTATATGGA TCTAAAGCTG GCCCAAGCAT CAGACAACTG 
AAATCTGGTT ATGTTGCTCA AACTGGTGGT AATAGTGGGC AAGGAGAAAA ACCCAATGGG 
TATGGTGCTG TGGCTGGCCC AAGCACAGGA CAACAAATGA GAAATGGTTA CAATCTTCAA 
TCTGGAGGAC AAACGACTAA AGGCAATGGT CAAGGCGTGG TGGCTGGACC CTCTACGGCA 
AATGGAGCAT ATGGACAAGG AGGAATGGGA AGCAAACTTA TGAAGGGTTA TGGCCGAACA 
CCTTATGGAT CAGGTGTTCA GAGGGGAGTG TTGGGAGGCC TCGGAGTTCC TCGGCCTGCT 
AGGAACCAGC AGAAATCGTA TGGGAGCAGA ACTGGTGACA GAGGTCAGCC CAACAGCGGC 
TACAATGGCG GTTATGGCAG CACAGCTCTG GGACCTCGTT ATGGAAATGG CGGGATGAAA 
GGACCGGTAA AAGGCTATGC AAACTTACCA CATACTGGTA ATAGAGGAGC AGTTCTAAAC 
GGTTATGAAC AACAAGGCGG TGGAGTAGCT GCAAAACCAG CAAAATCAGG TTTTGGAGCC 
ACAGCTGAAG GCGGAGTTTT CAAAGGATAT GGCACTAAAC CAAGTGGTCA TGGTGGAAAT 
GGTGCTGTTC TGAGTGGATA TGGACGACAG CCCAACGGTT ACAACCTGAT GCCAAATGGA 
AAGGGCCAGG CTGTTAGAGG AACAGAGGTA TCAGGTGAAA GGAACCTCAA AGGTGGTGTG 
CACTTCCACC ACCATCATCA TCAGCAGCAG CAGATGACGG CACAGTTGGG GGATGTCACC 
CCACGAGCAG CTACCCCACC TATCCCGGCC CCTACAGGTG GTCTCCTTGT TATGGTGACC 
CAAGAGAAGT ACCAAAAGCT GCCTTCACCT TTGCCCCAGG GGAAAAAGTA CAAACACACA 
GCAATCCTGC CTGAGGAAAG CCCAGAACCA GCACCTGCAT ATCCTGAAGC CAGAGGGCAG 
CACCTCTCAC TGGAGCCTGC ACCTATTGGT CCAAAGGACA AGAATCAGAA GGGGGTCCAT 
TCTGGACCTG CAGTCGTGGT TCCCCAAAGC AAACCCGCAC CAGAACCAGC AGCTGTTCAA 
GGTTATGTGA CTGGAAATGC TGTTCATCTG CAAAAAGCTG CAGAAGCAGA CGCTGTAGGT 
TTGGGGGGGG GCGCCCCAAA AACCAAAGCT GTTCCTGAAA CAGCTGAGGA AAGGGGCGGT 
GTCCCTCCAA AGGAAACACT GATCACTGCG GGTGTGGGCG AGTCTGCAGC AGAGGAGGCT 
GACGCACAAC CTGACAGGCT GCAGGGCACA AGTATCCATG CTGGGGAGAC GGGTACTGAA 
CCACACCTAG GTTTGGAGAG TAGGGGATCT TCTGCCTCAA AGGGACCAGG AGCCAAACTT 
GACTGTGGAC TGAATGGTCA GTGGATGAAA ATACCAAGAC CTGATTACAA TGCAGCAGCT 
GGTAGTTTGG ATGGCTCACA AACTAAAGGT TATGGAGCAG GAGAAAATGC TTTTCTAAAC 
AATGGATATG GAGCAGGGCT TGGGTACCCG TATGCAGGGA AAGCAGCTCA AGCTGGATAT 
GGAAAAGGAG TTCACCCTGG GGCTGGATAT GAAAATGGAA ATCCATATGG AGCTCTCAGT 
GAAGCAGGCT ATAGAAATGG GTATGGAGCA GGACCTCAGC CTGACTATTC AAGTTATGGT 
CAAGGTGTGC CGACTATTGA TGGCAAATCA GGTGGAAACC CAGGACTTCC TGCAGGACTA 
GATGGAATGA GTCAGTTTGA GCCTCAGTCT GTTGGTCTGG GTCCAAATGG AAAAGGCGTC 
ATGTATGGTG TTGGGGGGCT GCAGTTTGGT GGACAACCTG GGAACTCCGG GATAAATGGT 
GCAGGACAGT ATGGAGGTGT CGTTGCAAGT ATGGGAGGGA GTCTTGTGCC TGGAATGCAC 
AGTTATGGTG GATATGCTTA TAACCAAAAT GCTCCTGGAA AACAGGGTTA TGGAAGACTT 
CCTTATGGAG TTCAACAGGC TGGACTTAAC CCTGAGGCTA AAGCCGCCGC CCAGTATGGT 
CATACTGGGT CATCATATCA GCCTGAATCT GTTGGCTTCG GACAACCAGG GAAAACAGCA 
GGATCATACA GCGGGGGACA GGCTGCCTTT GCACCTCAGG CTCTTGGGTT TGGGGGTGAT 
GATAAATCTG GTAAATATGA TAAACAGGGA GCTTTCCAAT CACAGCGTCT CCGGCCTGAA 
GAAACAGCCG GTCAGACATA TGAGCGCCAG TCTGTTGGGT TGGATTCAGC AGGAAAGTCC 
TATGTGAAAG GGGAAGTGCC AACTGCAACT CTAGCAGCTG AAAGTGAGAG GATTCCCATT 
AATAAATATG AAAATGTGGG CTACATAAAT GGACAAATGC AGCCAGAAGT TGCTGGGCTT 
CCTGCAGCTC CCACTGCCAG TTCCACCCTG GCCTTTTCCT CTCCTGATGG GATTCCTGTA 
GAGGATGTTC AGGACATTCC TGATCCAGAG GGCACAGCCA GCTTCTTGGA GTCGGGACCC 
GTTACAGAAA TGCTGGGCGA GTCTCGGGGA CCGGAGGATG CCGAGAACCT GCCACGTCAG 
ATACACATCC AGCAGCATCT CAAACTCCAT TTCAACCCAC AAGGCAAAAA AAATGGCAAA 
TATGATGCAA ATGGCTTCTT TGGGAACAAT GGCTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101174706 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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