LOC101171180 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101171180
Entrez Gene ID	101171180
Full Name	FH1/FH2 domain-containing protein 3-like
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011481362.2	XP_011479664.1	FH1/FH2 domain-containing protein 3-like
XM_023960230.1	XP_023815998.1	FH1/FH2 domain-containing protein 3 isoform X1
XM_023960235.1	XP_023816003.1	FH1/FH2 domain-containing protein 3 isoform X6
XM_023960231.1	XP_023815999.1	FH1/FH2 domain-containing protein 3 isoform X2
XM_023960234.1	XP_023816002.1	FH1/FH2 domain-containing protein 3 isoform X5
XM_023960232.1	XP_023816000.1	FH1/FH2 domain-containing protein 3 isoform X3
XM_023960233.1	XP_023816001.1	FH1/FH2 domain-containing protein 3 isoform X4

Pathways from BioSystems
Gene Ontology

The following LOC101171180 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101171180 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa107319	XM_023960234.1 Latest version!	Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OJa107320	XM_023960235.1 Latest version!	Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OJa107315	XM_023960230.1 Latest version!	Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa107316	XM_023960231.1 Latest version!	Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa107317	XM_023960232.1 Latest version!	Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa107318	XM_023960233.1 Latest version!	Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OJa33837	XM_011481362.2 Latest version!	Oryzias latipes FH1/FH2 domain-containing protein 3-like (LOC101171180), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101171180 ( XM_023960234.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023816002.1 Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OJa107319
Clone ID Related Accession (Same CDS sequence)	XM_023960234.1
Accession Version	XM_023960234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	FH1/FH2 domain-containing protein 3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019869.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC TCGTCTGCAG GGTTCAGTTC TTAGATGACA CCGATCCGTT CAACAGCACC 
AACTTCCCGG AGCCCACGCG CCCGCCGCTC TACACCTTCA GGGAGGACAT CCCCCTCATC 
AACCAGCTCG CGGGCATCCA CCGGCTCCTC AAGGCTCCTC ACAAGCTCGA TGACTGCACA 
CTTCAGCTCT CCCACAATGG GACCTATCTG GATCTGGAGT CCTCCCTGGC CGAGCAAAGG 
GACGAGCTGG AGGGCTTTCA GCCCGACGAC ACTGGGTCCA GGGGGAGGAA GCACAGTGTC 
ATTCTACGGA CTCAGCTGAC GGTGCGCGTG CACGCCTGCA TCGAAAAACT CTACAACTGC 
AGTGGGCGGG ACTTAAGGAG GGCGCTGTTC TCCTTGAAGC AGATTTTTCA GGATGACAAA 
GATCTGGTCC ATGAGTTTGT GATGGCTGAA GGTCTGACGT GTCTCATCAA GGTGGGAGCA 
GAAGCCGACC AGAACTATCA GAACTACATT CTGAGAGCTC TGGGGCAGAT CATGCTGTAC 
GTGGACGGCA TGAACGGAGT CATCAGCCAC CCCGAGACCA TCCAGTGGCT CTACACGCTG 
GTCGGCTCAA AGTTTCGGTT GGTGGTGAAA ACGGCTCTGA AGCTGCTGCT GGTGTTTGTG 
GAGTACTCAG AGTCCAACGC GGCGCTGCTC ATTCAGGCCA TCGCCTCCGT GGACACGAAG 
GGAGGCTGCA AACCGTGGTC CAATGCCATG GAGATCCTAC ATGAGAAGGA TGGGGTGGAC 
ACGGAGTTGC TGGTCTACGC CATGACCCTC ATTAATAAGA CTTTGGCGGC ACTACCGGAT 
CAGGACTCCT TCTATGACAC GGTGGACAGC TTGGAGGAGC AAGGCATGGA GGCGGTCACC 
CAGAGGCACC TGGGGAGGAG AGGCACCGAC CTGGACCTGG TTGAGCAGCT CAACATTTAT 
GAGATGACCC TACGGCACGA AGATGGGGAC GACTGCCAGC CCCCATCCAC AGGTCGCCGG 
GACCGCCAGC GGGCCAGCCT CGGGGGAGGG GACAAACATC AAGGTCTGGA GAGGAGACGG 
AGCCGGCGGG CATCTCTTGG TCGATCGGGT CAGGCTTCTC CGTGCAGCCC AGCGTCACCG 
AGAGCCGGCT TTCATCCCTT CAGCGGACAC ACGGTTGAAG ACCTGAGTGA AAGAGAGGAG 
GAGGAGGAGG AGGAGGAGAC TCCTGTGACT GAGTCAGATT CGGATGAGCA GGAGGGGGCT 
CATCAGATCA CCTCCAGGAA CGGAGATCCT GCGTCTCACA CCTCGTTCAG CTCGCCAAGC 
ACGCCCACCA GCTCCAGACC GGCTCTGGGA GGACTGCTGT CATCCTCCTA CAGGCAGCAC 
CAGGAGTCTC TGGCTGCTGA GCGAGAGCGC CGCCGTGTGG AGCGAGAGGA GCGACTGCTC 
AGGATAGAGA GAGAGGAGAG AAACAAGCAC AGCCGCGACT ATGTGGATAA AATGGAAGAG 
GCCAGACTTG CGCGAGAGGA GAGGTATAAG AACGTGGAGC GCTTGGCCGC AGAGGAGTAT 
GAGAAGGAGC GTGTCCGAGG GTCAAGGACT CGCCCTGACC TCAACCTAAG CTTTGAGCCC 
TCCGAGGCCT GGCCTTCATC GCCGCGCAGC AGCACCCCAT CTTCCTTCGC CTCGCAGGAG 
GACGCAGAGG CAGAGGTGGA GGCGGTGACG CCAGTCGCGC TCTCTGAGAC GGGGAAAGCC 
ACAGATTCAA GCGACGGCGG CGGAGTGGAA GCGGAGGACA GAGAGGAAGA GCCCGCTGCT 
GAAGAGGAGG ACGAACACAA AGAAGAGGAG GGAGAACAGG AGGAAGCGGC AGCAGAGAAG 
GAAGAGGAGG AAGCTGAGGA GCAGGAGCCG CAGAAGCAGA GAGAAGAGGA GGTGGAGGAG 
GACAGTGGCA TCCTGAGTGA TAAGGAGCGA CAGAATGAGG AAGTAAACGA GAAAGACAAC 
TGCTCAGCGT CCAGCATCTC CTCTGCCAGC AGCACTTTAG AGCGAGAGGA GAGGGGAACC 
GGGGAGAATG GCCCCCCAGG CCTAAAGGAA GCAGAAGTCA GCGACAGTTG CAACAAACTC 
CTCAACAGCA AACTCTTCAT GCTGGACATG CTCTACTCCC AAAACAAGAA ACCATGCGAG 
GAGGAGGAGG AGGAAGAGGA GGAGGTGGAG AAGGAGAAGG AGAAAGGAGA AGGTGAGGAC 
AGAGAGACAG ACCAGGATGC CAGCGGAGAA CGCGGCGACG GGGAAAACAA GAAGGACGAC 
AAGTCAGACC ATCGCGGGAG CATCCGTCAG ATTGCCGAGC GGTTCGGAGA CCTGATCAAG 
GACGCCGGCT TGCCCCACCC CCACCCGGAC GACCCGGTCA ATGAGCTCCC TCCGCCCCCC 
GCGCCCAAGA AAGAGTCGGA CACCATCTGG GACCAGCTCA TGTCCAGCCC CCGAGAGCTG 
CGCATCGGAG ACGCCAACTT CACCGACCTG ACGGATGAGG ACGACAAGGA CATTCTGGAC 
GCTGTTCTGA TGGGAGGATG CAGTGACCTC CCACCCCCGC CGCCCCCTCT ACCCTTCATC 
CCACGCTTTC CACCACCTCC ACCAGTGCTA GGCATCTGCC CCTTGCCCCC TCCTTTGATG 
GGCAGCATGA GGCCTCCACC TCCACCGTCC TTTAGCTCTC CGATGCCAGG GCTGGCCCCC 
CAGGAGCAGC CTCTGTTCAA CAAAAAGAAG AAGACCATCC GACTCTTCTG GAGTGAAGTT 
CGGCCCACAG ACTGTCAGTA CAGGGACTAC AAACGAGGGG GAGACTCATT CTGGTCCAAA 
CTGGACACAG TCAAGCTGGA CACAGCCAAA CTGGAGAACC TGTTTGAGAC CAAGTCAAAG 
GAGATATCAG TTACAAAGAA AACAGCAGCT GATGGCAAAC GTCAGGAGAT CATCGTTTTG 
GACTCCAAGA GAAGCAACGC CATCAACATC GGGCTGACGG TCCTGCCGCC GCCTCGAACC 
ATCAAGACAG CCATCCTCAA CTTTGACGAA TACGCTCTCA ACAAGGAAGG CATAGAGAAA 
CTCCTGACAA TGATCCCAAC GGAGGAGGAG AAGCAGAAGA TCCAAGAGGC CCAGTTGGCC 
AATCCGGATG TGCCGTTGGG TTCTGCAGAG CAGTTTCTCC TCACTCTGTC CTCCATCAGT 
GAGCTCTCTG CCAGACTCCA CCTCTGGGCC TTTAAGATGG ACCAAGAAGC TACGGAAAAG 
GAAGTGGTGG AGCCACTGCA AGATCTGAAG GAGGGGATGG AGCAACTGGA GAAAAACAAA 
ACTCTGCGCT ACATCCTCTC CACCCTGCTC TCCATTGGAA ACTTCCTCAA CGGCACCAAT 
GCCAAAGGCT TTGAGCTGAC CTACCTGGAG AAGGTTCCAG AAGTGAAGGA CACGGTTCAC 
AAGCAGTCGC TGCTCCACCA TGTCTGCACG GTGGTGGTGG AAAACTTCCC CCAGAGCACC 
GACCTGTACT CAGAGATCGG AGCGATCACC CGCTCAGCAA AGGTGGATTT CGACCAGCTG 
CAGGAAAACC TGTGCCAGAT GGAGCGGCGC TGCAAAGCCT CATGGGACCA TCTGAAAGTC 
ATTGCCAAGC ATGAGATGAA GCCTCAGCTA AAACAGAAGA TGTCCGACTT CCTTAAAGAC 
TGCGCCGAGA GGATCATTAT CCTGAAGACG GTTCACCGAA GGATCATCAA CAGGTTCCAC 
TCCTTCCTGC TGTACCTCGG CCATCCTCCC TACAGCGTCA GAGAGATCAG CGTCAACAGG 
TTCAGCAAAA TCCTGAGCGA GTTTGCGCTG GAGTACCGAA CCACCCGCGA GCGCGTGCTG 
CAGCAGAAGC AGAAGCGAGC CGACCACCGC GAACGCAACA AGACCCGAGG AAAGATGATC 
ATGGACGTAA ACGCTCCTTC TGATGATGAA GAAGAGCGTG AGCATTCTGG TCCCGGCGGC 
TCAAGCAGCA GCAGCGGCGC CCCCAGTGGC ACCCAGGAGG TGGAGCAGCC TCAGGGGCTG 
GGCCACGCCG AGGACGCCGC AGAGCACGAG CACATGACAG CAGTGCTGAG GAGCAGCCTG 
AGCGGCAGCG CCGATAGAGA GGGCGGAGTG CCGGGTCTGC GCACCAGAAC CAGGTCCCGG 
CCTGGACGAG GAGGGCGCAG CATGCAGGCG TGGACTCCAC CCGCAGAGGA CGCTCAGACA 
TGTGGAGACG ATGCTGCAGA CGAGATCATG GAGCGGATCG TCCGGTCTGC CACACAGGGT 
CCAGGAACCA GAACACAGCC CAGAGAGCGC AGGAGGTCCA GGGCCAACCG GAAATCATTG 
AGACGGACTC TGAAGAACGG CCTCAGCCCA GAGGAAGCGC TGGCTTTAGG CCTGACCGAT 
TCTCCAGAGA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023816002.1
CDS	443..4837
Translation	MATLVCRVQFLDDTDPFNSTNFPEPTRPPLYTFREDIPLINQLAGIHRLLKAPHKLDDCTLQLSHNGTYLDLESSLAEQRDELEGFQPDDTGSRGRKHSVILRTQLTVRVHACIEKLYNCSGRDLRRALFSLKQIFQDDKDLVHEFVMAEGLTCLIKVGAEADQNYQNYILRALGQIMLYVDGMNGVISHPETIQWLYTLVGSKFRLVVKTALKLLLVFVEYSESNAALLIQAIASVDTKGGCKPWSNAMEILHEKDGVDTELLVYAMTLINKTLAALPDQDSFYDTVDSLEEQGMEAVTQRHLGRRGTDLDLVEQLNIYEMTLRHEDGDDCQPPSTGRRDRQRASLGGGDKHQGLERRRSRRASLGRSGQASPCSPASPRAGFHPFSGHTVEDLSEREEEEEEEETPVTESDSDEQEGAHQITSRNGDPASHTSFSSPSTPTSSRPALGGLLSSSYRQHQESLAAERERRRVEREERLLRIEREERNKHSRDYVDKMEEARLAREERYKNVERLAAEEYEKERVRGSRTRPDLNLSFEPSEAWPSSPRSSTPSSFASQEDAEAEVEAVTPVALSETGKATDSSDGGGVEAEDREEEPAAEEEDEHKEEEGEQEEAAAEKEEEEAEEQEPQKQREEEVEEDSGILSDKERQNEEVNEKDNCSASSISSASSTLEREERGTGENGPPGLKEAEVSDSCNKLLNSKLFMLDMLYSQNKKPCEEEEEEEEEVEKEKEKGEGEDRETDQDASGERGDGENKKDDKSDHRGSIRQIAERFGDLIKDAGLPHPHPDDPVNELPPPPAPKKESDTIWDQLMSSPRELRIGDANFTDLTDEDDKDILDAVLMGGCSDLPPPPPPLPFIPRFPPPPPVLGICPLPPPLMGSMRPPPPPSFSSPMPGLAPQEQPLFNKKKKTIRLFWSEVRPTDCQYRDYKRGGDSFWSKLDTVKLDTAKLENLFETKSKEISVTKKTAADGKRQEIIVLDSKRSNAINIGLTVLPPPRTIKTAILNFDEYALNKEGIEKLLTMIPTEEEKQKIQEAQLANPDVPLGSAEQFLLTLSSISELSARLHLWAFKMDQEATEKEVVEPLQDLKEGMEQLEKNKTLRYILSTLLSIGNFLNGTNAKGFELTYLEKVPEVKDTVHKQSLLHHVCTVVVENFPQSTDLYSEIGAITRSAKVDFDQLQENLCQMERRCKASWDHLKVIAKHEMKPQLKQKMSDFLKDCAERIIILKTVHRRIINRFHSFLLYLGHPPYSVREISVNRFSKILSEFALEYRTTRERVLQQKQKRADHRERNKTRGKMIMDVNAPSDDEEEREHSGPGGSSSSSGAPSGTQEVEQPQGLGHAEDAAEHEHMTAVLRSSLSGSADREGGVPGLRTRTRSRPGRGGRSMQAWTPPAEDAQTCGDDAADEIMERIVRSATQGPGTRTQPRERRRSRANRKSLRRTLKNGLSPEEALALGLTDSPET

Target ORF information:

RefSeq Version	XM_023960234.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023960234.1

ORF Insert Sequence:

ATGGCCACGC TCGTCTGCAG GGTTCAGTTC TTAGATGACA CCGATCCGTT CAACAGCACC 
AACTTCCCGG AGCCCACGCG CCCGCCGCTC TACACCTTCA GGGAGGACAT CCCCCTCATC 
AACCAGCTCG CGGGCATCCA CCGGCTCCTC AAGGCTCCTC ACAAGCTCGA TGACTGCACA 
CTTCAGCTCT CCCACAATGG GACCTATCTG GATCTGGAGT CCTCCCTGGC CGAGCAAAGG 
GACGAGCTGG AGGGCTTTCA GCCCGACGAC ACTGGGTCCA GGGGGAGGAA GCACAGTGTC 
ATTCTACGGA CTCAGCTGAC GGTGCGCGTG CACGCCTGCA TCGAAAAACT CTACAACTGC 
AGTGGGCGGG ACTTAAGGAG GGCGCTGTTC TCCTTGAAGC AGATTTTTCA GGATGACAAA 
GATCTGGTCC ATGAGTTTGT GATGGCTGAA GGTCTGACGT GTCTCATCAA GGTGGGAGCA 
GAAGCCGACC AGAACTATCA GAACTACATT CTGAGAGCTC TGGGGCAGAT CATGCTGTAC 
GTGGACGGCA TGAACGGAGT CATCAGCCAC CCCGAGACCA TCCAGTGGCT CTACACGCTG 
GTCGGCTCAA AGTTTCGGTT GGTGGTGAAA ACGGCTCTGA AGCTGCTGCT GGTGTTTGTG 
GAGTACTCAG AGTCCAACGC GGCGCTGCTC ATTCAGGCCA TCGCCTCCGT GGACACGAAG 
GGAGGCTGCA AACCGTGGTC CAATGCCATG GAGATCCTAC ATGAGAAGGA TGGGGTGGAC 
ACGGAGTTGC TGGTCTACGC CATGACCCTC ATTAATAAGA CTTTGGCGGC ACTACCGGAT 
CAGGACTCCT TCTATGACAC GGTGGACAGC TTGGAGGAGC AAGGCATGGA GGCGGTCACC 
CAGAGGCACC TGGGGAGGAG AGGCACCGAC CTGGACCTGG TTGAGCAGCT CAACATTTAT 
GAGATGACCC TACGGCACGA AGATGGGGAC GACTGCCAGC CCCCATCCAC AGGTCGCCGG 
GACCGCCAGC GGGCCAGCCT CGGGGGAGGG GACAAACATC AAGGTCTGGA GAGGAGACGG 
AGCCGGCGGG CATCTCTTGG TCGATCGGGT CAGGCTTCTC CGTGCAGCCC AGCGTCACCG 
AGAGCCGGCT TTCATCCCTT CAGCGGACAC ACGGTTGAAG ACCTGAGTGA AAGAGAGGAG 
GAGGAGGAGG AGGAGGAGAC TCCTGTGACT GAGTCAGATT CGGATGAGCA GGAGGGGGCT 
CATCAGATCA CCTCCAGGAA CGGAGATCCT GCGTCTCACA CCTCGTTCAG CTCGCCAAGC 
ACGCCCACCA GCTCCAGACC GGCTCTGGGA GGACTGCTGT CATCCTCCTA CAGGCAGCAC 
CAGGAGTCTC TGGCTGCTGA GCGAGAGCGC CGCCGTGTGG AGCGAGAGGA GCGACTGCTC 
AGGATAGAGA GAGAGGAGAG AAACAAGCAC AGCCGCGACT ATGTGGATAA AATGGAAGAG 
GCCAGACTTG CGCGAGAGGA GAGGTATAAG AACGTGGAGC GCTTGGCCGC AGAGGAGTAT 
GAGAAGGAGC GTGTCCGAGG GTCAAGGACT CGCCCTGACC TCAACCTAAG CTTTGAGCCC 
TCCGAGGCCT GGCCTTCATC GCCGCGCAGC AGCACCCCAT CTTCCTTCGC CTCGCAGGAG 
GACGCAGAGG CAGAGGTGGA GGCGGTGACG CCAGTCGCGC TCTCTGAGAC GGGGAAAGCC 
ACAGATTCAA GCGACGGCGG CGGAGTGGAA GCGGAGGACA GAGAGGAAGA GCCCGCTGCT 
GAAGAGGAGG ACGAACACAA AGAAGAGGAG GGAGAACAGG AGGAAGCGGC AGCAGAGAAG 
GAAGAGGAGG AAGCTGAGGA GCAGGAGCCG CAGAAGCAGA GAGAAGAGGA GGTGGAGGAG 
GACAGTGGCA TCCTGAGTGA TAAGGAGCGA CAGAATGAGG AAGTAAACGA GAAAGACAAC 
TGCTCAGCGT CCAGCATCTC CTCTGCCAGC AGCACTTTAG AGCGAGAGGA GAGGGGAACC 
GGGGAGAATG GCCCCCCAGG CCTAAAGGAA GCAGAAGTCA GCGACAGTTG CAACAAACTC 
CTCAACAGCA AACTCTTCAT GCTGGACATG CTCTACTCCC AAAACAAGAA ACCATGCGAG 
GAGGAGGAGG AGGAAGAGGA GGAGGTGGAG AAGGAGAAGG AGAAAGGAGA AGGTGAGGAC 
AGAGAGACAG ACCAGGATGC CAGCGGAGAA CGCGGCGACG GGGAAAACAA GAAGGACGAC 
AAGTCAGACC ATCGCGGGAG CATCCGTCAG ATTGCCGAGC GGTTCGGAGA CCTGATCAAG 
GACGCCGGCT TGCCCCACCC CCACCCGGAC GACCCGGTCA ATGAGCTCCC TCCGCCCCCC 
GCGCCCAAGA AAGAGTCGGA CACCATCTGG GACCAGCTCA TGTCCAGCCC CCGAGAGCTG 
CGCATCGGAG ACGCCAACTT CACCGACCTG ACGGATGAGG ACGACAAGGA CATTCTGGAC 
GCTGTTCTGA TGGGAGGATG CAGTGACCTC CCACCCCCGC CGCCCCCTCT ACCCTTCATC 
CCACGCTTTC CACCACCTCC ACCAGTGCTA GGCATCTGCC CCTTGCCCCC TCCTTTGATG 
GGCAGCATGA GGCCTCCACC TCCACCGTCC TTTAGCTCTC CGATGCCAGG GCTGGCCCCC 
CAGGAGCAGC CTCTGTTCAA CAAAAAGAAG AAGACCATCC GACTCTTCTG GAGTGAAGTT 
CGGCCCACAG ACTGTCAGTA CAGGGACTAC AAACGAGGGG GAGACTCATT CTGGTCCAAA 
CTGGACACAG TCAAGCTGGA CACAGCCAAA CTGGAGAACC TGTTTGAGAC CAAGTCAAAG 
GAGATATCAG TTACAAAGAA AACAGCAGCT GATGGCAAAC GTCAGGAGAT CATCGTTTTG 
GACTCCAAGA GAAGCAACGC CATCAACATC GGGCTGACGG TCCTGCCGCC GCCTCGAACC 
ATCAAGACAG CCATCCTCAA CTTTGACGAA TACGCTCTCA ACAAGGAAGG CATAGAGAAA 
CTCCTGACAA TGATCCCAAC GGAGGAGGAG AAGCAGAAGA TCCAAGAGGC CCAGTTGGCC 
AATCCGGATG TGCCGTTGGG TTCTGCAGAG CAGTTTCTCC TCACTCTGTC CTCCATCAGT 
GAGCTCTCTG CCAGACTCCA CCTCTGGGCC TTTAAGATGG ACCAAGAAGC TACGGAAAAG 
GAAGTGGTGG AGCCACTGCA AGATCTGAAG GAGGGGATGG AGCAACTGGA GAAAAACAAA 
ACTCTGCGCT ACATCCTCTC CACCCTGCTC TCCATTGGAA ACTTCCTCAA CGGCACCAAT 
GCCAAAGGCT TTGAGCTGAC CTACCTGGAG AAGGTTCCAG AAGTGAAGGA CACGGTTCAC 
AAGCAGTCGC TGCTCCACCA TGTCTGCACG GTGGTGGTGG AAAACTTCCC CCAGAGCACC 
GACCTGTACT CAGAGATCGG AGCGATCACC CGCTCAGCAA AGGTGGATTT CGACCAGCTG 
CAGGAAAACC TGTGCCAGAT GGAGCGGCGC TGCAAAGCCT CATGGGACCA TCTGAAAGTC 
ATTGCCAAGC ATGAGATGAA GCCTCAGCTA AAACAGAAGA TGTCCGACTT CCTTAAAGAC 
TGCGCCGAGA GGATCATTAT CCTGAAGACG GTTCACCGAA GGATCATCAA CAGGTTCCAC 
TCCTTCCTGC TGTACCTCGG CCATCCTCCC TACAGCGTCA GAGAGATCAG CGTCAACAGG 
TTCAGCAAAA TCCTGAGCGA GTTTGCGCTG GAGTACCGAA CCACCCGCGA GCGCGTGCTG 
CAGCAGAAGC AGAAGCGAGC CGACCACCGC GAACGCAACA AGACCCGAGG AAAGATGATC 
ATGGACGTAA ACGCTCCTTC TGATGATGAA GAAGAGCGTG AGCATTCTGG TCCCGGCGGC 
TCAAGCAGCA GCAGCGGCGC CCCCAGTGGC ACCCAGGAGG TGGAGCAGCC TCAGGGGCTG 
GGCCACGCCG AGGACGCCGC AGAGCACGAG CACATGACAG CAGTGCTGAG GAGCAGCCTG 
AGCGGCAGCG CCGATAGAGA GGGCGGAGTG CCGGGTCTGC GCACCAGAAC CAGGTCCCGG 
CCTGGACGAG GAGGGCGCAG CATGCAGGCG TGGACTCCAC CCGCAGAGGA CGCTCAGACA 
TGTGGAGACG ATGCTGCAGA CGAGATCATG GAGCGGATCG TCCGGTCTGC CACACAGGGT 
CCAGGAACCA GAACACAGCC CAGAGAGCGC AGGAGGTCCA GGGCCAACCG GAAATCATTG 
AGACGGACTC TGAAGAACGG CCTCAGCCCA GAGGAAGCGC TGGCTTTAGG CCTGACCGAT 
TCTCCAGAGA CGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101171180 ( XM_023960235.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023816003.1 Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OJa107320
Clone ID Related Accession (Same CDS sequence)	XM_023960235.1
Accession Version	XM_023960235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	FH1/FH2 domain-containing protein 3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019869.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC TCGTCTGCAG GGTTCAGTTC TTAGATGACA CCGATCCGTT CAACAGCACC 
AACTTCCCGG AGCCCACGCG CCCGCCGCTC TACACCTTCA GGGAGGACAT CCCCCTCATC 
AACCAGCTCG CGGGCATCCA CCGGCTCCTC AAGGCTCCTC ACAAGCTCGA TGACTGCACA 
CTTCAGCTCT CCCACAATGG GACCTATCTG GATCTGGAGT CCTCCCTGGC CGAGCAAAGG 
GACGAGCTGG AGGGCTTTCA GCCCGACGAC ACTGGGTCCA GGGGGAGGAA GCACAGTGTC 
ATTCTACGGA CTCAGCTGAC GGTGCGCGTG CACGCCTGCA TCGAAAAACT CTACAACTGC 
AGTGGGCGGG ACTTAAGGAG GGCGCTGTTC TCCTTGAAGC AGATTTTTCA GGATGACAAA 
GATCTGGTCC ATGAGTTTGT GATGGCTGAA GGTCTGACGT GTCTCATCAA GGTGGGAGCA 
GAAGCCGACC AGAACTATCA GAACTACATT CTGAGAGCTC TGGGGCAGAT CATGCTGTAC 
GTGGACGGCA TGAACGGAGT CATCAGCCAC CCCGAGACCA TCCAGTGGCT CTACACGCTG 
GTCGGCTCAA AGTTTCGGTT GGTGGTGAAA ACGGCTCTGA AGCTGCTGCT GGTGTTTGTG 
GAGTACTCAG AGTCCAACGC GGCGCTGCTC ATTCAGGCCA TCGCCTCCGT GGACACGAAG 
GGAGGCTGCA AACCGTGGTC CAATGCCATG GAGATCCTAC ATGAGAAGGA TGGGGTGGAC 
ACGGAGTTGC TGGTCTACGC CATGACCCTC ATTAATAAGA CTTTGGCGGC ACTACCGGAT 
CAGGACTCCT TCTATGACAC GGTGGACAGC TTGGAGGAGC AAGGCATGGA GGCGGTCACC 
CAGAGGCACC TGGGGAGGAG AGGCACCGAC CTGGACCTGG TTGAGCAGCT CAACATTTAT 
GAGATGACCC TACGGCACGA AGATGGGGAC GACTGCCAGC CCCCATCCAC AGGTCGCCGG 
GACCGCCAGC GGGCCAGCCT CGGGGGAGGG GACAAACATC AAGGTCTGGA GAGGAGACGG 
AGCCGGCGGG CATCTCTTGG TCGATCGGGT CAGGCTTCTC CGTGCAGCCC AGCGTCACCG 
AGAGCCGGCT TTCATCCCTT CAGCGGACAC ACGGTTGAAG ACCTGAGTGA AAGGAACGGA 
GATCCTGCGT CTCACACCTC GTTCAGCTCG CCAAGCACGC CCACCAGCTC CAGACCGGCT 
CTGGGAGGAC TGCTGTCATC CTCCTACAGG CAGCACCAGG AGTCTCTGGC TGCTGAGCGA 
GAGCGCCGCC GTGTGGAGCG AGAGGAGCGA CTGCTCAGGA TAGAGAGAGA GGAGAGAAAC 
AAGCACAGCC GCGACTATGT GGATAAAATG GAAGAGGCCA GACTTGCGCG AGAGGAGAGG 
TATAAGAACG TGGAGCGCTT GGCCGCAGAG GAGTATGAGA AGGAGCGTGT CCGAGGGTCA 
AGGACTCGCC CTGACCTCAA CCTAAGCTTT GAGCCCTCCG AGGCCTGGCC TTCATCGCCG 
CGCAGCAGCA CCCCATCTTC CTTCGCCTCG CAGGAGGACG CAGAGGCAGA GGTGGAGGCG 
GTGACGCCAG TCGCGCTCTC TGAGACGGGG AAAGCCACAG ATTCAAGCGA CGGCGGCGGA 
GTGGAAGCGG AGGACAGAGA GGAAGAGCCC GCTGCTGAAG AGGAGGACGA ACACAAAGAA 
GAGGAGGGAG AACAGGAGGA AGCGGCAGCA GAGAAGGAAG AGGAGGAAGC TGAGGAGCAG 
GAGCCGCAGA AGCAGAGAGA AGAGGAGGTG GAGGAGGACA GTGGCATCCT GAGTGATAAG 
GAGCGACAGA ATGAGGAAGT AAACGAGAAA GACAACTGCT CAGCGTCCAG CATCTCCTCT 
GCCAGCAGCA CTTTAGAGCG AGAGGAGAGG GGAACCGGGG AGAATGGCCC CCCAGGCCTA 
AAGGAAGCAG AAGTCAGCGA CAGTTGCAAC AAACTCCTCA ACAGCAAACT CTTCATGCTG 
GACATGCTCT ACTCCCAAAA CAAGAAACCA TGCGAGGAGG AGGAGGAGGA AGAGGAGGAG 
GTGGAGAAGG AGAAGGAGAA AGGAGAAGGT GAGGACAGAG AGACAGACCA GGATGCCAGC 
GGAGAACGCG GCGACGGGGA AAACAAGAAG GACGACAAGT CAGACCATCG CGGGAGCATC 
CGTCAGATTG CCGAGCGGTT CGGAGACCTG ATCAAGGACG CCGGCTTGCC CCACCCCCAC 
CCGGACGACC CGGTCAATGA GCTCCCTCCG CCCCCCGCGC CCAAGAAAGA GTCGGACACC 
ATCTGGGACC AGCTCATGTC CAGCCCCCGA GAGCTGCGCA TCGGAGACGC CAACTTCACC 
GACCTGACGG ATGAGGACGA CAAGGACATT CTGGACGCTG TTCTGATGGG AGGATGCAGT 
GACCTCCCAC CCCCGCCGCC CCCTCTACCC TTCATCCCAC GCTTTCCACC ACCTCCACCA 
GTGCTAGGCA TCTGCCCCTT GCCCCCTCCT TTGATGGGCA GCATGAGGCC TCCACCTCCA 
CCGTCCTTTA GCTCTCCGAT GCCAGGGCTG GCCCCCCAGG AGCAGCCTCT GTTCAACAAA 
AAGAAGAAGA CCATCCGACT CTTCTGGAGT GAAGTTCGGC CCACAGACTG TCAGTACAGG 
GACTACAAAC GAGGGGGAGA CTCATTCTGG TCCAAACTGG ACACAGTCAA GCTGGACACA 
GCCAAACTGG AGAACCTGTT TGAGACCAAG TCAAAGGAGA TATCAGTTAC AAAGAAAACA 
GCAGCTGATG GCAAACGTCA GGAGATCATC GTTTTGGACT CCAAGAGAAG CAACGCCATC 
AACATCGGGC TGACGGTCCT GCCGCCGCCT CGAACCATCA AGACAGCCAT CCTCAACTTT 
GACGAATACG CTCTCAACAA GGAAGGCATA GAGAAACTCC TGACAATGAT CCCAACGGAG 
GAGGAGAAGC AGAAGATCCA AGAGGCCCAG TTGGCCAATC CGGATGTGCC GTTGGGTTCT 
GCAGAGCAGT TTCTCCTCAC TCTGTCCTCC ATCAGTGAGC TCTCTGCCAG ACTCCACCTC 
TGGGCCTTTA AGATGGACCA AGAAGCTACG GAAAAGGAAG TGGTGGAGCC ACTGCAAGAT 
CTGAAGGAGG GGATGGAGCA ACTGGAGAAA AACAAAACTC TGCGCTACAT CCTCTCCACC 
CTGCTCTCCA TTGGAAACTT CCTCAACGGC ACCAATGCCA AAGGCTTTGA GCTGACCTAC 
CTGGAGAAGG TTCCAGAAGT GAAGGACACG GTTCACAAGC AGTCGCTGCT CCACCATGTC 
TGCACGGTGG TGGTGGAAAA CTTCCCCCAG AGCACCGACC TGTACTCAGA GATCGGAGCG 
ATCACCCGCT CAGCAAAGGT GGATTTCGAC CAGCTGCAGG AAAACCTGTG CCAGATGGAG 
CGGCGCTGCA AAGCCTCATG GGACCATCTG AAAGTCATTG CCAAGCATGA GATGAAGCCT 
CAGCTAAAAC AGAAGATGTC CGACTTCCTT AAAGACTGCG CCGAGAGGAT CATTATCCTG 
AAGACGGTTC ACCGAAGGAT CATCAACAGG TTCCACTCCT TCCTGCTGTA CCTCGGCCAT 
CCTCCCTACA GCGTCAGAGA GATCAGCGTC AACAGGTTCA GCAAAATCCT GAGCGAGTTT 
GCGCTGGAGT ACCGAACCAC CCGCGAGCGC GTGCTGCAGC AGAAGCAGAA GCGAGCCGAC 
CACCGCGAAC GCAACAAGAC CCGAGGAAAG ATGATCATGG ACGTAAACGC TCCTTCTGAT 
GATGAAGAAG AGCGTGAGCA TTCTGGTCCC GGCGGCTCAA GCAGCAGCAG CGGCGCCCCC 
AGTGGCACCC AGGAGGTGGA GCAGCCTCAG GGGCTGGGCC ACGCCGAGGA CGCCGCAGAG 
CACGAGCACA TGACAGCAGT GCTGAGGAGC AGCCTGAGCG GCAGCGCCGA TAGAGAGGGC 
GGAGTGCCGG GTCTGCGCAC CAGAACCAGG TCCCGGCCTG GACGAGGAGG GCGCAGCATG 
CAGGCGTGGA CTCCACCCGC AGAGGACGCT CAGACATGTG GAGACGATGC TGCAGACGAG 
ATCATGGAGC GGATCGTCCG GTCTGCCACA CAGGGTCCAG GAACCAGAAC ACAGCCCAGA 
GAGCGCAGGA GGTCCAGGGC CAACCGGAAA TCATTGAGAC GGACTCTGAA GAACGGCCTC 
AGCCCAGAGG AAGCGCTGGC TTTAGGCCTG ACCGATTCTC CAGAGACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023816003.1
CDS	441..4751
Translation	MATLVCRVQFLDDTDPFNSTNFPEPTRPPLYTFREDIPLINQLAGIHRLLKAPHKLDDCTLQLSHNGTYLDLESSLAEQRDELEGFQPDDTGSRGRKHSVILRTQLTVRVHACIEKLYNCSGRDLRRALFSLKQIFQDDKDLVHEFVMAEGLTCLIKVGAEADQNYQNYILRALGQIMLYVDGMNGVISHPETIQWLYTLVGSKFRLVVKTALKLLLVFVEYSESNAALLIQAIASVDTKGGCKPWSNAMEILHEKDGVDTELLVYAMTLINKTLAALPDQDSFYDTVDSLEEQGMEAVTQRHLGRRGTDLDLVEQLNIYEMTLRHEDGDDCQPPSTGRRDRQRASLGGGDKHQGLERRRSRRASLGRSGQASPCSPASPRAGFHPFSGHTVEDLSERNGDPASHTSFSSPSTPTSSRPALGGLLSSSYRQHQESLAAERERRRVEREERLLRIEREERNKHSRDYVDKMEEARLAREERYKNVERLAAEEYEKERVRGSRTRPDLNLSFEPSEAWPSSPRSSTPSSFASQEDAEAEVEAVTPVALSETGKATDSSDGGGVEAEDREEEPAAEEEDEHKEEEGEQEEAAAEKEEEEAEEQEPQKQREEEVEEDSGILSDKERQNEEVNEKDNCSASSISSASSTLEREERGTGENGPPGLKEAEVSDSCNKLLNSKLFMLDMLYSQNKKPCEEEEEEEEEVEKEKEKGEGEDRETDQDASGERGDGENKKDDKSDHRGSIRQIAERFGDLIKDAGLPHPHPDDPVNELPPPPAPKKESDTIWDQLMSSPRELRIGDANFTDLTDEDDKDILDAVLMGGCSDLPPPPPPLPFIPRFPPPPPVLGICPLPPPLMGSMRPPPPPSFSSPMPGLAPQEQPLFNKKKKTIRLFWSEVRPTDCQYRDYKRGGDSFWSKLDTVKLDTAKLENLFETKSKEISVTKKTAADGKRQEIIVLDSKRSNAINIGLTVLPPPRTIKTAILNFDEYALNKEGIEKLLTMIPTEEEKQKIQEAQLANPDVPLGSAEQFLLTLSSISELSARLHLWAFKMDQEATEKEVVEPLQDLKEGMEQLEKNKTLRYILSTLLSIGNFLNGTNAKGFELTYLEKVPEVKDTVHKQSLLHHVCTVVVENFPQSTDLYSEIGAITRSAKVDFDQLQENLCQMERRCKASWDHLKVIAKHEMKPQLKQKMSDFLKDCAERIIILKTVHRRIINRFHSFLLYLGHPPYSVREISVNRFSKILSEFALEYRTTRERVLQQKQKRADHRERNKTRGKMIMDVNAPSDDEEEREHSGPGGSSSSSGAPSGTQEVEQPQGLGHAEDAAEHEHMTAVLRSSLSGSADREGGVPGLRTRTRSRPGRGGRSMQAWTPPAEDAQTCGDDAADEIMERIVRSATQGPGTRTQPRERRRSRANRKSLRRTLKNGLSPEEALALGLTDSPET

Target ORF information:

RefSeq Version	XM_023960235.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023960235.1

ORF Insert Sequence:

ATGGCCACGC TCGTCTGCAG GGTTCAGTTC TTAGATGACA CCGATCCGTT CAACAGCACC 
AACTTCCCGG AGCCCACGCG CCCGCCGCTC TACACCTTCA GGGAGGACAT CCCCCTCATC 
AACCAGCTCG CGGGCATCCA CCGGCTCCTC AAGGCTCCTC ACAAGCTCGA TGACTGCACA 
CTTCAGCTCT CCCACAATGG GACCTATCTG GATCTGGAGT CCTCCCTGGC CGAGCAAAGG 
GACGAGCTGG AGGGCTTTCA GCCCGACGAC ACTGGGTCCA GGGGGAGGAA GCACAGTGTC 
ATTCTACGGA CTCAGCTGAC GGTGCGCGTG CACGCCTGCA TCGAAAAACT CTACAACTGC 
AGTGGGCGGG ACTTAAGGAG GGCGCTGTTC TCCTTGAAGC AGATTTTTCA GGATGACAAA 
GATCTGGTCC ATGAGTTTGT GATGGCTGAA GGTCTGACGT GTCTCATCAA GGTGGGAGCA 
GAAGCCGACC AGAACTATCA GAACTACATT CTGAGAGCTC TGGGGCAGAT CATGCTGTAC 
GTGGACGGCA TGAACGGAGT CATCAGCCAC CCCGAGACCA TCCAGTGGCT CTACACGCTG 
GTCGGCTCAA AGTTTCGGTT GGTGGTGAAA ACGGCTCTGA AGCTGCTGCT GGTGTTTGTG 
GAGTACTCAG AGTCCAACGC GGCGCTGCTC ATTCAGGCCA TCGCCTCCGT GGACACGAAG 
GGAGGCTGCA AACCGTGGTC CAATGCCATG GAGATCCTAC ATGAGAAGGA TGGGGTGGAC 
ACGGAGTTGC TGGTCTACGC CATGACCCTC ATTAATAAGA CTTTGGCGGC ACTACCGGAT 
CAGGACTCCT TCTATGACAC GGTGGACAGC TTGGAGGAGC AAGGCATGGA GGCGGTCACC 
CAGAGGCACC TGGGGAGGAG AGGCACCGAC CTGGACCTGG TTGAGCAGCT CAACATTTAT 
GAGATGACCC TACGGCACGA AGATGGGGAC GACTGCCAGC CCCCATCCAC AGGTCGCCGG 
GACCGCCAGC GGGCCAGCCT CGGGGGAGGG GACAAACATC AAGGTCTGGA GAGGAGACGG 
AGCCGGCGGG CATCTCTTGG TCGATCGGGT CAGGCTTCTC CGTGCAGCCC AGCGTCACCG 
AGAGCCGGCT TTCATCCCTT CAGCGGACAC ACGGTTGAAG ACCTGAGTGA AAGGAACGGA 
GATCCTGCGT CTCACACCTC GTTCAGCTCG CCAAGCACGC CCACCAGCTC CAGACCGGCT 
CTGGGAGGAC TGCTGTCATC CTCCTACAGG CAGCACCAGG AGTCTCTGGC TGCTGAGCGA 
GAGCGCCGCC GTGTGGAGCG AGAGGAGCGA CTGCTCAGGA TAGAGAGAGA GGAGAGAAAC 
AAGCACAGCC GCGACTATGT GGATAAAATG GAAGAGGCCA GACTTGCGCG AGAGGAGAGG 
TATAAGAACG TGGAGCGCTT GGCCGCAGAG GAGTATGAGA AGGAGCGTGT CCGAGGGTCA 
AGGACTCGCC CTGACCTCAA CCTAAGCTTT GAGCCCTCCG AGGCCTGGCC TTCATCGCCG 
CGCAGCAGCA CCCCATCTTC CTTCGCCTCG CAGGAGGACG CAGAGGCAGA GGTGGAGGCG 
GTGACGCCAG TCGCGCTCTC TGAGACGGGG AAAGCCACAG ATTCAAGCGA CGGCGGCGGA 
GTGGAAGCGG AGGACAGAGA GGAAGAGCCC GCTGCTGAAG AGGAGGACGA ACACAAAGAA 
GAGGAGGGAG AACAGGAGGA AGCGGCAGCA GAGAAGGAAG AGGAGGAAGC TGAGGAGCAG 
GAGCCGCAGA AGCAGAGAGA AGAGGAGGTG GAGGAGGACA GTGGCATCCT GAGTGATAAG 
GAGCGACAGA ATGAGGAAGT AAACGAGAAA GACAACTGCT CAGCGTCCAG CATCTCCTCT 
GCCAGCAGCA CTTTAGAGCG AGAGGAGAGG GGAACCGGGG AGAATGGCCC CCCAGGCCTA 
AAGGAAGCAG AAGTCAGCGA CAGTTGCAAC AAACTCCTCA ACAGCAAACT CTTCATGCTG 
GACATGCTCT ACTCCCAAAA CAAGAAACCA TGCGAGGAGG AGGAGGAGGA AGAGGAGGAG 
GTGGAGAAGG AGAAGGAGAA AGGAGAAGGT GAGGACAGAG AGACAGACCA GGATGCCAGC 
GGAGAACGCG GCGACGGGGA AAACAAGAAG GACGACAAGT CAGACCATCG CGGGAGCATC 
CGTCAGATTG CCGAGCGGTT CGGAGACCTG ATCAAGGACG CCGGCTTGCC CCACCCCCAC 
CCGGACGACC CGGTCAATGA GCTCCCTCCG CCCCCCGCGC CCAAGAAAGA GTCGGACACC 
ATCTGGGACC AGCTCATGTC CAGCCCCCGA GAGCTGCGCA TCGGAGACGC CAACTTCACC 
GACCTGACGG ATGAGGACGA CAAGGACATT CTGGACGCTG TTCTGATGGG AGGATGCAGT 
GACCTCCCAC CCCCGCCGCC CCCTCTACCC TTCATCCCAC GCTTTCCACC ACCTCCACCA 
GTGCTAGGCA TCTGCCCCTT GCCCCCTCCT TTGATGGGCA GCATGAGGCC TCCACCTCCA 
CCGTCCTTTA GCTCTCCGAT GCCAGGGCTG GCCCCCCAGG AGCAGCCTCT GTTCAACAAA 
AAGAAGAAGA CCATCCGACT CTTCTGGAGT GAAGTTCGGC CCACAGACTG TCAGTACAGG 
GACTACAAAC GAGGGGGAGA CTCATTCTGG TCCAAACTGG ACACAGTCAA GCTGGACACA 
GCCAAACTGG AGAACCTGTT TGAGACCAAG TCAAAGGAGA TATCAGTTAC AAAGAAAACA 
GCAGCTGATG GCAAACGTCA GGAGATCATC GTTTTGGACT CCAAGAGAAG CAACGCCATC 
AACATCGGGC TGACGGTCCT GCCGCCGCCT CGAACCATCA AGACAGCCAT CCTCAACTTT 
GACGAATACG CTCTCAACAA GGAAGGCATA GAGAAACTCC TGACAATGAT CCCAACGGAG 
GAGGAGAAGC AGAAGATCCA AGAGGCCCAG TTGGCCAATC CGGATGTGCC GTTGGGTTCT 
GCAGAGCAGT TTCTCCTCAC TCTGTCCTCC ATCAGTGAGC TCTCTGCCAG ACTCCACCTC 
TGGGCCTTTA AGATGGACCA AGAAGCTACG GAAAAGGAAG TGGTGGAGCC ACTGCAAGAT 
CTGAAGGAGG GGATGGAGCA ACTGGAGAAA AACAAAACTC TGCGCTACAT CCTCTCCACC 
CTGCTCTCCA TTGGAAACTT CCTCAACGGC ACCAATGCCA AAGGCTTTGA GCTGACCTAC 
CTGGAGAAGG TTCCAGAAGT GAAGGACACG GTTCACAAGC AGTCGCTGCT CCACCATGTC 
TGCACGGTGG TGGTGGAAAA CTTCCCCCAG AGCACCGACC TGTACTCAGA GATCGGAGCG 
ATCACCCGCT CAGCAAAGGT GGATTTCGAC CAGCTGCAGG AAAACCTGTG CCAGATGGAG 
CGGCGCTGCA AAGCCTCATG GGACCATCTG AAAGTCATTG CCAAGCATGA GATGAAGCCT 
CAGCTAAAAC AGAAGATGTC CGACTTCCTT AAAGACTGCG CCGAGAGGAT CATTATCCTG 
AAGACGGTTC ACCGAAGGAT CATCAACAGG TTCCACTCCT TCCTGCTGTA CCTCGGCCAT 
CCTCCCTACA GCGTCAGAGA GATCAGCGTC AACAGGTTCA GCAAAATCCT GAGCGAGTTT 
GCGCTGGAGT ACCGAACCAC CCGCGAGCGC GTGCTGCAGC AGAAGCAGAA GCGAGCCGAC 
CACCGCGAAC GCAACAAGAC CCGAGGAAAG ATGATCATGG ACGTAAACGC TCCTTCTGAT 
GATGAAGAAG AGCGTGAGCA TTCTGGTCCC GGCGGCTCAA GCAGCAGCAG CGGCGCCCCC 
AGTGGCACCC AGGAGGTGGA GCAGCCTCAG GGGCTGGGCC ACGCCGAGGA CGCCGCAGAG 
CACGAGCACA TGACAGCAGT GCTGAGGAGC AGCCTGAGCG GCAGCGCCGA TAGAGAGGGC 
GGAGTGCCGG GTCTGCGCAC CAGAACCAGG TCCCGGCCTG GACGAGGAGG GCGCAGCATG 
CAGGCGTGGA CTCCACCCGC AGAGGACGCT CAGACATGTG GAGACGATGC TGCAGACGAG 
ATCATGGAGC GGATCGTCCG GTCTGCCACA CAGGGTCCAG GAACCAGAAC ACAGCCCAGA 
GAGCGCAGGA GGTCCAGGGC CAACCGGAAA TCATTGAGAC GGACTCTGAA GAACGGCCTC 
AGCCCAGAGG AAGCGCTGGC TTTAGGCCTG ACCGATTCTC CAGAGACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101171180 ( XM_023960230.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023815998.1 Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X1, mRNA.

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CloneID	OJa107315
Clone ID Related Accession (Same CDS sequence)	XM_023960230.1
Accession Version	XM_023960230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	FH1/FH2 domain-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019869.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC TCGTCTGCAG GGTTCAGTTC TTAGATGACA CCGATCCGTT CAACAGCACC 
AACTTCCCGG AGCCCACGCG CCCGCCGCTC TACACCTTCA GGGAGGACAT CCCCCTCATC 
AACCAGCTCG CGGGCATCCA CCGGCTCCTC AAGGCTCCTC ACAAGCTCGA TGACTGCACA 
CTTCAGCTCT CCCACAATGG GACCTATCTG GATCTGGAGT CCTCCCTGGC CGAGCAAAGG 
GACGAGCTGG AGGGCTTTCA GCCCGACGAC ACTGGGTCCA GGGGGAGGAA GCACAGTGTC 
ATTCTACGGA CTCAGCTGAC GGTGCGCGTG CACGCCTGCA TCGAAAAACT CTACAACTGC 
AGTGGGCGGG ACTTAAGGAG GGCGCTGTTC TCCTTGAAGC AGATTTTTCA GGATGACAAA 
GATCTGGTCC ATGAGTTTGT GATGGCTGAA GGTCTGACGT GTCTCATCAA GGTGGGAGCA 
GAAGCCGACC AGAACTATCA GAACTACATT CTGAGAGCTC TGGGGCAGAT CATGCTGTAC 
GTGGACGGCA TGAACGGAGT CATCAGCCAC CCCGAGACCA TCCAGTGGCT CTACACGCTG 
GTCGGCTCAA AGTTTCGGTT GGTGGTGAAA ACGGCTCTGA AGCTGCTGCT GGTGTTTGTG 
GAGTACTCAG AGTCCAACGC GGCGCTGCTC ATTCAGGCCA TCGCCTCCGT GGACACGAAG 
GGAGGCTGCA AACCGTGGTC CAATGCCATG GAGATCCTAC ATGAGAAGGA TGGGGTGGAC 
ACGGAGTTGC TGGTCTACGC CATGACCCTC ATTAATAAGA CTTTGGCGGC ACTACCGGAT 
CAGGACTCCT TCTATGACAC GGTGGACAGC TTGGAGGAGC AAGGCATGGA GGCGGTCACC 
CAGAGGCACC TGGGGAGGAG AGGCACCGAC CTGGACCTGG TTGAGCAGCT CAACATTTAT 
GAGATGACCC TACGGCACGA AGATGGGGAC GACTGCCAGC CCCCATCCAC AGGTCGCCGG 
GACCGCCAGC GGGCCAGCCT CGGGGGAGGG GACAAACATC AAGGTCTGGA GAGGAGACGG 
AGCCGGCGGG CATCTCTTGG TCGATCGGGT CAGGCTTCTC CGTGCAGCCC AGCGTCACCG 
AGAGCCGGCT TTCATCCCTT CAGCGGACAC ACGGTTGAAG ACCTGAGTGA AAGAGAGGAG 
GAGGAGGAGG AGGAGGAGAC TCCTGTGACT GAGTCAGATT CGGATGAGCA GGAGGGGGCT 
CATCAGATCA CCTCCAGTCC AAACTGGGGG GGCAAATACT TCTCTGTGAG TGACCCATCT 
GTTGACCCCT GTGTTTGTAG CCCCCCCAGA CCCCCTCCAC CCACCGCTCA CAGAACGAGC 
CATCGCTCCA TCACCTCCAG GAAGGAGAAA GAGCAGAAGA GCAGGACCCC TTCGCCCCCA 
GCAGCCCCAC CCTCACCATC TAAACCCCCC TGCAGCCCCC CAGCCCACCG CTCCCTGCTA 
TCTACCTTAC TGGCACGGAA GCGGTCCATC GTCACCGTTC CAGCCACCAC GGAGGTCAGT 
CCTCCTCTGC GCGGCAGCTC CCGCCCCTCC CCGGACCGCC TGCCCTACCT GCCCCACTCC 
CCCTTCCACC TCTTCTCATA CGATCTTGAG GAGGAGGCGT CCGCTTCGGC CCAGACCAAA 
CGCAGCACGG AGTCGGCATC TCCAAGGAAC GGAGATCCTG CGTCTCACAC CTCGTTCAGC 
TCGCCAAGCA CGCCCACCAG CTCCAGACCG GCTCTGGGAG GACTGCTGTC ATCCTCCTAC 
AGGCAGCACC AGGAGTCTCT GGCTGCTGAG CGAGAGCGCC GCCGTGTGGA GCGAGAGGAG 
CGACTGCTCA GGATAGAGAG AGAGGAGAGA AACAAGCACA GCCGCGACTA TGTGGATAAA 
ATGGAAGAGG CCAGACTTGC GCGAGAGGAG AGGTATAAGA ACGTGGAGCG CTTGGCCGCA 
GAGGAGTATG AGAAGGAGCG TGTCCGAGGG TCAAGGACTC GCCCTGACCT CAACCTAAGC 
TTTGAGCCCT CCGAGGCCTG GCCTTCATCG CCGCGCAGCA GCACCCCATC TTCCTTCGCC 
TCGCAGGAGG ACGCAGAGGC AGAGGTGGAG GCGGTGACGC CAGTCGCGCT CTCTGAGACG 
GGGAAAGCCA CAGATTCAAG CGACGGCGGC GGAGTGGAAG CGGAGGACAG AGAGGAAGAG 
CCCGCTGCTG AAGAGGAGGA CGAACACAAA GAAGAGGAGG GAGAACAGGA GGAAGCGGCA 
GCAGAGAAGG AAGAGGAGGA AGCTGAGGAG CAGGAGCCGC AGAAGCAGAG AGAAGAGGAG 
GTGGAGGAGG ACAGTGGCAT CCTGAGTGAT AAGGAGCGAC AGAATGAGGA AGTAAACGAG 
AAAGACAACT GCTCAGCGTC CAGCATCTCC TCTGCCAGCA GCACTTTAGA GCGAGAGGAG 
AGGGGAACCG GGGAGAATGG CCCCCCAGGC CTAAAGGAAG CAGAAGTCAG CGACAGTTGC 
AACAAACTCC TCAACAGCAA ACTCTTCATG CTGGACATGC TCTACTCCCA AAACAAGAAA 
CCATGCGAGG AGGAGGAGGA GGAAGAGGAG GAGGTGGAGA AGGAGAAGGA GAAAGGAGAA 
GGTGAGGACA GAGAGACAGA CCAGGATGCC AGCGGAGAAC GCGGCGACGG GGAAAACAAG 
AAGGACGACA AGTCAGACCA TCGCGGGAGC ATCCGTCAGA TTGCCGAGCG GTTCGGAGAC 
CTGATCAAGG ACGCCGGCTT GCCCCACCCC CACCCGGACG ACCCGGTCAA TGAGCTCCCT 
CCGCCCCCCG CGCCCAAGAA AGAGTCGGAC ACCATCTGGG ACCAGCTCAT GTCCAGCCCC 
CGAGAGCTGC GCATCGGAGA CGCCAACTTC ACCGACCTGA CGGATGAGGA CGACAAGGAC 
ATTCTGGACG CTGTTCTGAT GGGAGGATGC AGTGACCTCC CACCCCCGCC GCCCCCTCTA 
CCCTTCATCC CACGCTTTCC ACCACCTCCA CCAGTGCTAG GCATCTGCCC CTTGCCCCCT 
CCTTTGATGG GCAGCATGAG GCCTCCACCT CCACCGTCCT TTAGCTCTCC GATGCCAGGG 
CTGGCCCCCC AGGAGCAGCC TCTGTTCAAC AAAAAGAAGA AGACCATCCG ACTCTTCTGG 
AGTGAAGTTC GGCCCACAGA CTGTCAGTAC AGGGACTACA AACGAGGGGG AGACTCATTC 
TGGTCCAAAC TGGACACAGT CAAGCTGGAC ACAGCCAAAC TGGAGAACCT GTTTGAGACC 
AAGTCAAAGG AGATATCAGT TACAAAGAAA ACAGCAGCTG ATGGCAAACG TCAGGAGATC 
ATCGTTTTGG ACTCCAAGAG AAGCAACGCC ATCAACATCG GGCTGACGGT CCTGCCGCCG 
CCTCGAACCA TCAAGACAGC CATCCTCAAC TTTGACGAAT ACGCTCTCAA CAAGGAAGGC 
ATAGAGAAAC TCCTGACAAT GATCCCAACG GAGGAGGAGA AGCAGAAGAT CCAAGAGGCC 
CAGTTGGCCA ATCCGGATGT GCCGTTGGGT TCTGCAGAGC AGTTTCTCCT CACTCTGTCC 
TCCATCAGTG AGCTCTCTGC CAGACTCCAC CTCTGGGCCT TTAAGATGGA CCAAGAAGCT 
ACGGAAAAGG AAGTGGTGGA GCCACTGCAA GATCTGAAGG AGGGGATGGA GCAACTGGAG 
AAAAACAAAA CTCTGCGCTA CATCCTCTCC ACCCTGCTCT CCATTGGAAA CTTCCTCAAC 
GGCACCAATG CCAAAGGCTT TGAGCTGACC TACCTGGAGA AGGTTCCAGA AGTGAAGGAC 
ACGGTTCACA AGCAGTCGCT GCTCCACCAT GTCTGCACGG TGGTGGTGGA AAACTTCCCC 
CAGAGCACCG ACCTGTACTC AGAGATCGGA GCGATCACCC GCTCAGCAAA GGTGGATTTC 
GACCAGCTGC AGGAAAACCT GTGCCAGATG GAGCGGCGCT GCAAAGCCTC ATGGGACCAT 
CTGAAAGTCA TTGCCAAGCA TGAGATGAAG CCTCAGCTAA AACAGAAGAT GTCCGACTTC 
CTTAAAGACT GCGCCGAGAG GATCATTATC CTGAAGACGG TTCACCGAAG GATCATCAAC 
AGGTTCCACT CCTTCCTGCT GTACCTCGGC CATCCTCCCT ACAGCGTCAG AGAGATCAGC 
GTCAACAGGT TCAGCAAAAT CCTGAGCGAG TTTGCGCTGG AGTACCGAAC CACCCGCGAG 
CGCGTGCTGC AGCAGAAGCA GAAGCGAGCC GACCACCGCG AACGCAACAA GACCCGAGGA 
AAGATGATCA TGGACGTAAA CGCTCCTTCT GATGATGAAG AAGAGCGTGA GCATTCTGGT 
CCCGGCGGCT CAAGCAGCAG CAGCGGCGCC CCCAGTGGCA CCCAGGAGGT GGAGCAGCCT 
CAGGGGCTGG GCCACGCCGA GGACGCCGCA GAGCACGAGC ACATGACAGC AGTGCTGAGG 
AGCAGCCTGA GCGGCAGCGC CGATAGAGAG GGCGGAGTGC CGGGTCTGCG CACCAGAACC 
AGGTCCCGGC CTGGACGAGG AGGGCGCAGC ATGCAGGCGT GGACTCCACC CGCAGAGGAC 
GCTCAGACAT GTGGAGACGA TGCTGCAGAC GAGATCATGG AGCGGATCGT CCGGTCTGCC 
ACACAGGGTC CAGGAACCAG AACACAGCCC AGAGAGCGCA GGAGGTCCAG GGCCAACCGG 
AAATCATTGA GACGGACTCT GAAGAACGGC CTCAGCCCAG AGGAAGCGCT GGCTTTAGGC 
CTGACCGATT CTCCAGAGAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023815998.1
CDS	450..5273
Translation	MATLVCRVQFLDDTDPFNSTNFPEPTRPPLYTFREDIPLINQLAGIHRLLKAPHKLDDCTLQLSHNGTYLDLESSLAEQRDELEGFQPDDTGSRGRKHSVILRTQLTVRVHACIEKLYNCSGRDLRRALFSLKQIFQDDKDLVHEFVMAEGLTCLIKVGAEADQNYQNYILRALGQIMLYVDGMNGVISHPETIQWLYTLVGSKFRLVVKTALKLLLVFVEYSESNAALLIQAIASVDTKGGCKPWSNAMEILHEKDGVDTELLVYAMTLINKTLAALPDQDSFYDTVDSLEEQGMEAVTQRHLGRRGTDLDLVEQLNIYEMTLRHEDGDDCQPPSTGRRDRQRASLGGGDKHQGLERRRSRRASLGRSGQASPCSPASPRAGFHPFSGHTVEDLSEREEEEEEEETPVTESDSDEQEGAHQITSSPNWGGKYFSVSDPSVDPCVCSPPRPPPPTAHRTSHRSITSRKEKEQKSRTPSPPAAPPSPSKPPCSPPAHRSLLSTLLARKRSIVTVPATTEVSPPLRGSSRPSPDRLPYLPHSPFHLFSYDLEEEASASAQTKRSTESASPRNGDPASHTSFSSPSTPTSSRPALGGLLSSSYRQHQESLAAERERRRVEREERLLRIEREERNKHSRDYVDKMEEARLAREERYKNVERLAAEEYEKERVRGSRTRPDLNLSFEPSEAWPSSPRSSTPSSFASQEDAEAEVEAVTPVALSETGKATDSSDGGGVEAEDREEEPAAEEEDEHKEEEGEQEEAAAEKEEEEAEEQEPQKQREEEVEEDSGILSDKERQNEEVNEKDNCSASSISSASSTLEREERGTGENGPPGLKEAEVSDSCNKLLNSKLFMLDMLYSQNKKPCEEEEEEEEEVEKEKEKGEGEDRETDQDASGERGDGENKKDDKSDHRGSIRQIAERFGDLIKDAGLPHPHPDDPVNELPPPPAPKKESDTIWDQLMSSPRELRIGDANFTDLTDEDDKDILDAVLMGGCSDLPPPPPPLPFIPRFPPPPPVLGICPLPPPLMGSMRPPPPPSFSSPMPGLAPQEQPLFNKKKKTIRLFWSEVRPTDCQYRDYKRGGDSFWSKLDTVKLDTAKLENLFETKSKEISVTKKTAADGKRQEIIVLDSKRSNAINIGLTVLPPPRTIKTAILNFDEYALNKEGIEKLLTMIPTEEEKQKIQEAQLANPDVPLGSAEQFLLTLSSISELSARLHLWAFKMDQEATEKEVVEPLQDLKEGMEQLEKNKTLRYILSTLLSIGNFLNGTNAKGFELTYLEKVPEVKDTVHKQSLLHHVCTVVVENFPQSTDLYSEIGAITRSAKVDFDQLQENLCQMERRCKASWDHLKVIAKHEMKPQLKQKMSDFLKDCAERIIILKTVHRRIINRFHSFLLYLGHPPYSVREISVNRFSKILSEFALEYRTTRERVLQQKQKRADHRERNKTRGKMIMDVNAPSDDEEEREHSGPGGSSSSSGAPSGTQEVEQPQGLGHAEDAAEHEHMTAVLRSSLSGSADREGGVPGLRTRTRSRPGRGGRSMQAWTPPAEDAQTCGDDAADEIMERIVRSATQGPGTRTQPRERRRSRANRKSLRRTLKNGLSPEEALALGLTDSPET

Target ORF information:

RefSeq Version	XM_023960230.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023960230.1

ORF Insert Sequence:

ATGGCCACGC TCGTCTGCAG GGTTCAGTTC TTAGATGACA CCGATCCGTT CAACAGCACC 
AACTTCCCGG AGCCCACGCG CCCGCCGCTC TACACCTTCA GGGAGGACAT CCCCCTCATC 
AACCAGCTCG CGGGCATCCA CCGGCTCCTC AAGGCTCCTC ACAAGCTCGA TGACTGCACA 
CTTCAGCTCT CCCACAATGG GACCTATCTG GATCTGGAGT CCTCCCTGGC CGAGCAAAGG 
GACGAGCTGG AGGGCTTTCA GCCCGACGAC ACTGGGTCCA GGGGGAGGAA GCACAGTGTC 
ATTCTACGGA CTCAGCTGAC GGTGCGCGTG CACGCCTGCA TCGAAAAACT CTACAACTGC 
AGTGGGCGGG ACTTAAGGAG GGCGCTGTTC TCCTTGAAGC AGATTTTTCA GGATGACAAA 
GATCTGGTCC ATGAGTTTGT GATGGCTGAA GGTCTGACGT GTCTCATCAA GGTGGGAGCA 
GAAGCCGACC AGAACTATCA GAACTACATT CTGAGAGCTC TGGGGCAGAT CATGCTGTAC 
GTGGACGGCA TGAACGGAGT CATCAGCCAC CCCGAGACCA TCCAGTGGCT CTACACGCTG 
GTCGGCTCAA AGTTTCGGTT GGTGGTGAAA ACGGCTCTGA AGCTGCTGCT GGTGTTTGTG 
GAGTACTCAG AGTCCAACGC GGCGCTGCTC ATTCAGGCCA TCGCCTCCGT GGACACGAAG 
GGAGGCTGCA AACCGTGGTC CAATGCCATG GAGATCCTAC ATGAGAAGGA TGGGGTGGAC 
ACGGAGTTGC TGGTCTACGC CATGACCCTC ATTAATAAGA CTTTGGCGGC ACTACCGGAT 
CAGGACTCCT TCTATGACAC GGTGGACAGC TTGGAGGAGC AAGGCATGGA GGCGGTCACC 
CAGAGGCACC TGGGGAGGAG AGGCACCGAC CTGGACCTGG TTGAGCAGCT CAACATTTAT 
GAGATGACCC TACGGCACGA AGATGGGGAC GACTGCCAGC CCCCATCCAC AGGTCGCCGG 
GACCGCCAGC GGGCCAGCCT CGGGGGAGGG GACAAACATC AAGGTCTGGA GAGGAGACGG 
AGCCGGCGGG CATCTCTTGG TCGATCGGGT CAGGCTTCTC CGTGCAGCCC AGCGTCACCG 
AGAGCCGGCT TTCATCCCTT CAGCGGACAC ACGGTTGAAG ACCTGAGTGA AAGAGAGGAG 
GAGGAGGAGG AGGAGGAGAC TCCTGTGACT GAGTCAGATT CGGATGAGCA GGAGGGGGCT 
CATCAGATCA CCTCCAGTCC AAACTGGGGG GGCAAATACT TCTCTGTGAG TGACCCATCT 
GTTGACCCCT GTGTTTGTAG CCCCCCCAGA CCCCCTCCAC CCACCGCTCA CAGAACGAGC 
CATCGCTCCA TCACCTCCAG GAAGGAGAAA GAGCAGAAGA GCAGGACCCC TTCGCCCCCA 
GCAGCCCCAC CCTCACCATC TAAACCCCCC TGCAGCCCCC CAGCCCACCG CTCCCTGCTA 
TCTACCTTAC TGGCACGGAA GCGGTCCATC GTCACCGTTC CAGCCACCAC GGAGGTCAGT 
CCTCCTCTGC GCGGCAGCTC CCGCCCCTCC CCGGACCGCC TGCCCTACCT GCCCCACTCC 
CCCTTCCACC TCTTCTCATA CGATCTTGAG GAGGAGGCGT CCGCTTCGGC CCAGACCAAA 
CGCAGCACGG AGTCGGCATC TCCAAGGAAC GGAGATCCTG CGTCTCACAC CTCGTTCAGC 
TCGCCAAGCA CGCCCACCAG CTCCAGACCG GCTCTGGGAG GACTGCTGTC ATCCTCCTAC 
AGGCAGCACC AGGAGTCTCT GGCTGCTGAG CGAGAGCGCC GCCGTGTGGA GCGAGAGGAG 
CGACTGCTCA GGATAGAGAG AGAGGAGAGA AACAAGCACA GCCGCGACTA TGTGGATAAA 
ATGGAAGAGG CCAGACTTGC GCGAGAGGAG AGGTATAAGA ACGTGGAGCG CTTGGCCGCA 
GAGGAGTATG AGAAGGAGCG TGTCCGAGGG TCAAGGACTC GCCCTGACCT CAACCTAAGC 
TTTGAGCCCT CCGAGGCCTG GCCTTCATCG CCGCGCAGCA GCACCCCATC TTCCTTCGCC 
TCGCAGGAGG ACGCAGAGGC AGAGGTGGAG GCGGTGACGC CAGTCGCGCT CTCTGAGACG 
GGGAAAGCCA CAGATTCAAG CGACGGCGGC GGAGTGGAAG CGGAGGACAG AGAGGAAGAG 
CCCGCTGCTG AAGAGGAGGA CGAACACAAA GAAGAGGAGG GAGAACAGGA GGAAGCGGCA 
GCAGAGAAGG AAGAGGAGGA AGCTGAGGAG CAGGAGCCGC AGAAGCAGAG AGAAGAGGAG 
GTGGAGGAGG ACAGTGGCAT CCTGAGTGAT AAGGAGCGAC AGAATGAGGA AGTAAACGAG 
AAAGACAACT GCTCAGCGTC CAGCATCTCC TCTGCCAGCA GCACTTTAGA GCGAGAGGAG 
AGGGGAACCG GGGAGAATGG CCCCCCAGGC CTAAAGGAAG CAGAAGTCAG CGACAGTTGC 
AACAAACTCC TCAACAGCAA ACTCTTCATG CTGGACATGC TCTACTCCCA AAACAAGAAA 
CCATGCGAGG AGGAGGAGGA GGAAGAGGAG GAGGTGGAGA AGGAGAAGGA GAAAGGAGAA 
GGTGAGGACA GAGAGACAGA CCAGGATGCC AGCGGAGAAC GCGGCGACGG GGAAAACAAG 
AAGGACGACA AGTCAGACCA TCGCGGGAGC ATCCGTCAGA TTGCCGAGCG GTTCGGAGAC 
CTGATCAAGG ACGCCGGCTT GCCCCACCCC CACCCGGACG ACCCGGTCAA TGAGCTCCCT 
CCGCCCCCCG CGCCCAAGAA AGAGTCGGAC ACCATCTGGG ACCAGCTCAT GTCCAGCCCC 
CGAGAGCTGC GCATCGGAGA CGCCAACTTC ACCGACCTGA CGGATGAGGA CGACAAGGAC 
ATTCTGGACG CTGTTCTGAT GGGAGGATGC AGTGACCTCC CACCCCCGCC GCCCCCTCTA 
CCCTTCATCC CACGCTTTCC ACCACCTCCA CCAGTGCTAG GCATCTGCCC CTTGCCCCCT 
CCTTTGATGG GCAGCATGAG GCCTCCACCT CCACCGTCCT TTAGCTCTCC GATGCCAGGG 
CTGGCCCCCC AGGAGCAGCC TCTGTTCAAC AAAAAGAAGA AGACCATCCG ACTCTTCTGG 
AGTGAAGTTC GGCCCACAGA CTGTCAGTAC AGGGACTACA AACGAGGGGG AGACTCATTC 
TGGTCCAAAC TGGACACAGT CAAGCTGGAC ACAGCCAAAC TGGAGAACCT GTTTGAGACC 
AAGTCAAAGG AGATATCAGT TACAAAGAAA ACAGCAGCTG ATGGCAAACG TCAGGAGATC 
ATCGTTTTGG ACTCCAAGAG AAGCAACGCC ATCAACATCG GGCTGACGGT CCTGCCGCCG 
CCTCGAACCA TCAAGACAGC CATCCTCAAC TTTGACGAAT ACGCTCTCAA CAAGGAAGGC 
ATAGAGAAAC TCCTGACAAT GATCCCAACG GAGGAGGAGA AGCAGAAGAT CCAAGAGGCC 
CAGTTGGCCA ATCCGGATGT GCCGTTGGGT TCTGCAGAGC AGTTTCTCCT CACTCTGTCC 
TCCATCAGTG AGCTCTCTGC CAGACTCCAC CTCTGGGCCT TTAAGATGGA CCAAGAAGCT 
ACGGAAAAGG AAGTGGTGGA GCCACTGCAA GATCTGAAGG AGGGGATGGA GCAACTGGAG 
AAAAACAAAA CTCTGCGCTA CATCCTCTCC ACCCTGCTCT CCATTGGAAA CTTCCTCAAC 
GGCACCAATG CCAAAGGCTT TGAGCTGACC TACCTGGAGA AGGTTCCAGA AGTGAAGGAC 
ACGGTTCACA AGCAGTCGCT GCTCCACCAT GTCTGCACGG TGGTGGTGGA AAACTTCCCC 
CAGAGCACCG ACCTGTACTC AGAGATCGGA GCGATCACCC GCTCAGCAAA GGTGGATTTC 
GACCAGCTGC AGGAAAACCT GTGCCAGATG GAGCGGCGCT GCAAAGCCTC ATGGGACCAT 
CTGAAAGTCA TTGCCAAGCA TGAGATGAAG CCTCAGCTAA AACAGAAGAT GTCCGACTTC 
CTTAAAGACT GCGCCGAGAG GATCATTATC CTGAAGACGG TTCACCGAAG GATCATCAAC 
AGGTTCCACT CCTTCCTGCT GTACCTCGGC CATCCTCCCT ACAGCGTCAG AGAGATCAGC 
GTCAACAGGT TCAGCAAAAT CCTGAGCGAG TTTGCGCTGG AGTACCGAAC CACCCGCGAG 
CGCGTGCTGC AGCAGAAGCA GAAGCGAGCC GACCACCGCG AACGCAACAA GACCCGAGGA 
AAGATGATCA TGGACGTAAA CGCTCCTTCT GATGATGAAG AAGAGCGTGA GCATTCTGGT 
CCCGGCGGCT CAAGCAGCAG CAGCGGCGCC CCCAGTGGCA CCCAGGAGGT GGAGCAGCCT 
CAGGGGCTGG GCCACGCCGA GGACGCCGCA GAGCACGAGC ACATGACAGC AGTGCTGAGG 
AGCAGCCTGA GCGGCAGCGC CGATAGAGAG GGCGGAGTGC CGGGTCTGCG CACCAGAACC 
AGGTCCCGGC CTGGACGAGG AGGGCGCAGC ATGCAGGCGT GGACTCCACC CGCAGAGGAC 
GCTCAGACAT GTGGAGACGA TGCTGCAGAC GAGATCATGG AGCGGATCGT CCGGTCTGCC 
ACACAGGGTC CAGGAACCAG AACACAGCCC AGAGAGCGCA GGAGGTCCAG GGCCAACCGG 
AAATCATTGA GACGGACTCT GAAGAACGGC CTCAGCCCAG AGGAAGCGCT GGCTTTAGGC 
CTGACCGATT CTCCAGAGAC GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101171180 ( XM_023960231.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023815999.1 Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X2, mRNA.

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CloneID	OJa107316
Clone ID Related Accession (Same CDS sequence)	XM_023960231.1
Accession Version	XM_023960231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4800bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	FH1/FH2 domain-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019869.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC TCGTCTGCAG GGTTCAGTTC TTAGATGACA CCGATCCGTT CAACAGCACC 
AACTTCCCGG AGCCCACGCG CCCGCCGCTC TACACCTTCA GGGAGGACAT CCCCCTCATC 
AACCAGCTCG CGGGCATCCA CCGGCTCCTC AAGGCTCCTC ACAAGCTCGA TGACTGCACA 
CTTCAGCTCT CCCACAATGG GACCTATCTG GATCTGGAGT CCTCCCTGGC CGAGCAAAGG 
GACGAGCTGG AGGGCTTTCA GCCCGACGAC ACTGGGTCCA GGGGGAGGAA GCACAGTGTC 
ATTCTACGGA CTCAGCTGAC GGTGCGCGTG CACGCCTGCA TCGAAAAACT CTACAACTGC 
AGTGGGCGGG ACTTAAGGAG GGCGCTGTTC TCCTTGAAGC AGATTTTTCA GGATGACAAA 
GATCTGGTCC ATGAGTTTGT GATGGCTGAA GGTCTGACGT GTCTCATCAA GGTGGGAGCA 
GAAGCCGACC AGAACTATCA GAACTACATT CTGAGAGCTC TGGGGCAGAT CATGCTGTAC 
GTGGACGGCA TGAACGGAGT CATCAGCCAC CCCGAGACCA TCCAGTGGCT CTACACGCTG 
GTCGGCTCAA AGTTTCGGTT GGTGGTGAAA ACGGCTCTGA AGCTGCTGCT GGTGTTTGTG 
GAGTACTCAG AGTCCAACGC GGCGCTGCTC ATTCAGGCCA TCGCCTCCGT GGACACGAAG 
GGAGGCTGCA AACCGTGGTC CAATGCCATG GAGATCCTAC ATGAGAAGGA TGGGGTGGAC 
ACGGAGTTGC TGGTCTACGC CATGACCCTC ATTAATAAGA CTTTGGCGGC ACTACCGGAT 
CAGGACTCCT TCTATGACAC GGTGGACAGC TTGGAGGAGC AAGGCATGGA GGCGGTCACC 
CAGAGGCACC TGGGGAGGAG AGGCACCGAC CTGGACCTGG TTGAGCAGCT CAACATTTAT 
GAGATGACCC TACGGCACGA AGATGGGGAC GACTGCCAGC CCCCATCCAC AGGTCGCCGG 
GACCGCCAGC GGGCCAGCCT CGGGGGAGGG GACAAACATC AAGGTCTGGA GAGGAGACGG 
AGCCGGCGGG CATCTCTTGG TCGATCGGGT CAGGCTTCTC CGTGCAGCCC AGCGTCACCG 
AGAGCCGGCT TTCATCCCTT CAGCGGACAC ACGGTTGAAG ACCTGAGTGA AAGAGAGGAG 
GAGGAGGAGG AGGAGGAGAC TCCTGTGACT GAGTCAGATT CGGATGAGCA GGAGGGGGCT 
CATCAGATCA CCTCCAGTCC AAACTGGGGG GGCAAATACT TCTCTGTGAG TGACCCATCT 
GTTGACCCCT GTGTTTGTAG CCCCCCCAGA CCCCCTCCAC CCACCGCTCA CAGAACGAGC 
CATCGCTCCA TCACCTCCAG GAAGGAGAAA GAGCAGAAGA GCAGGACCCC TTCGCCCCCA 
GCAGCCCCAC CCTCACCATC TAAACCCCCC TGCAGCCCCC CAGCCCACCG CTCCCTGCTA 
TCTACCTTAC TGGCACGGAA GCGGTCCATC GTCACCGTTC CAGCCACCAC GGAGGTCAGT 
CCTCCTCTGC GCGGCAGCTC CCGCCCCTCC CCGGACCGCC TGCCCTACCT GCCCCACTCC 
CCCTTCCACC TCTTCTCATA CGATCTTGAG GAGGAGGCGT CCGCTTCGGC CCAGACCAAA 
CGCAGCACGG AGTCGGCATC TCCAAGGAAC GGAGATCCTG CGTCTCACAC CTCGTTCAGC 
TCGCCAAGCA CGCCCACCAG CTCCAGACCG GCTCTGGGAG GACTGCTGTC ATCCTCCTAC 
AGGCAGCACC AGGAGTCTCT GGCTGCTGAG CGAGAGCGCC GCCGTGTGGA GCGAGAGGAG 
CGACTGCTCA GGATAGAGAG AGAGGAGAGA AACAAGCACA GCCGCGACTA TGTGGATAAA 
ATGGAAGAGG CCAGACTTGC GCGAGAGGAG AGGTATAAGA ACGTGGAGCG CTTGGCCGCA 
GAGGAGTATG AGAAGGAGCG TGTCCGAGGG TCAAGGACTC GCCCTGACCT CAACCTAAGC 
TTTGAGCCCT CCGAGGCCTG GCCTTCATCG CCGCGCAGCA GCACCCCATC TTCCTTCGCC 
TCGCAGGAGG ACGCAGAGGC AGAGGTGGAG GCGGTGACGC CAGTCGCGCT CTCTGAGACG 
GGGAAAGCCA CAGATTCAAG CGACGGCGGC GGAGTGGAAG CGGAGGACAG AGAGGAAGAG 
CCCGCTGCTG AAGAGGAGGA CGAACACAAA GAAGAGGAGG GAGAACAGGA GGAAGCGGCA 
GCAGAGAAGG AAGAGGAGGA AGCTGAGGAG CAGGAGCCGC AGAAGCAGAG AGAAGAGGAG 
GTGGAGGAGG ACAGTGGCAT CCTGAGTGAT AAGGAGCGAC AGAATGAGGA AGTAAACGAG 
AAAGACAACT GCTCAGCGTC CAGCATCTCC TCTGCCAGCA GCACTTTAGA GCGAGAGGAG 
AGGGGAACCG GGGAGAATGG CCCCCCAGGC CTAAAGGAAG CAGAAGTCAG CGACAGTTGC 
AACAAACTCC TCAACAGCAA ACTCTTCATG CTGGACATGC TCTACTCCCA AAACAAGAAA 
CCATGCGAGG AGGAGGAGGA GGAAGAGGAG GAGGTGGAGA AGGAGAAGGA GAAAGGAGAA 
GGTGAGGACA GAGAGACAGA CCAGGATGCC AGCGGAGAAC GCGGCGACGG GGAAAACAAG 
AAGGACGACA AGTCAGACCA TCGCGGGAGC ATCCGTCAGA TTGCCGAGCG GTTCGGAGAC 
CTGATCAAGG ACGCCGGCTT GCCCCACCCC CACCCGGACG ACCCGGTCAA TGAGCTCCCT 
CCGCCCCCCG CGCCCAAGAA AGAGTCGGAC ACCATCTGGG ACCAGCTCAT GTCCAGCCCC 
CGAGAGCTGC GCATCGGAGA CGCCAACTTC ACCGACCTGA CGGATGAGGA CGACAAGGAC 
ATTCTGGACG CTGTTCTGAT GGGAGGATGC AGTGACCTCC CACCCCCGCC GCCCCCTCTA 
CCCTTCATCC CACGCTTTCC ACCACCTCCA CCAGTGCTAG GCATCTGCCC CTTGCCCCCT 
CCTTTGATGG GCAGCATGAG GCCTCCACCT CCACCGTCCT TTAGCTCTCC GATGCCAGGG 
CTGGCCCCCC AGGAGCAGCC TCTGTTCAAC AAAAAGAAGA AGACCATCCG ACTCTTCTGG 
AGTGAAGTTC GGCCCACAGA CTGTCAGTAC AGGGACTACA AACGAGGGGG AGACTCATTC 
TGGTCCAAAC TGGACACAGT CAAGCTGGAC ACAGCCAAAC TGGAGAACCT GTTTGAGACC 
AAGTCAAAGG AGATATCAGT TACAAAGAAA ACAGCAGCTG ATGGCAAACG TCAGGAGATC 
ATCGTTTTGG ACTCCAAGAG AAGCAACGCC ATCAACATCG GGCTGACGGT CCTGCCGCCG 
CCTCGAACCA TCAAGACAGC CATCCTCAAC TTTGACGAAT ACGCTCTCAA CAAGGAAGGC 
ATAGAGAAAC TCCTGACAAT GATCCCAACG GAGGAGGAGA AGCAGAAGAT CCAAGAGGCC 
CAGTTGGCCA ATCCGGATGT GCCGTTGGGT TCTGCAGAGC AGTTTCTCCT CACTCTGTCC 
TCCATCAGTG AGCTCTCTGC CAGACTCCAC CTCTGGGCCT TTAAGATGGA CCAAGAAGCT 
ACGGAAAAGG AAGTGGTGGA GCCACTGCAA GATCTGAAGG AGGGGATGGA GCAACTGGAG 
AAAAACAAAA CTCTGCGCTA CATCCTCTCC ACCCTGCTCT CCATTGGAAA CTTCCTCAAC 
GGCACCAATG CCAAAGGCTT TGAGCTGACC TACCTGGAGA AGGTTCCAGA AGTGAAGGAC 
ACGGTTCACA AGCAGTCGCT GCTCCACCAT GTCTGCACGG TGGTGGTGGA AAACTTCCCC 
CAGAGCACCG ACCTGTACTC AGAGATCGGA GCGATCACCC GCTCAGCAAA GGTGGATTTC 
GACCAGCTGC AGGAAAACCT GTGCCAGATG GAGCGGCGCT GCAAAGCCTC ATGGGACCAT 
CTGAAAGTCA TTGCCAAGCA TGAGATGAAG CCTCAGCTAA AACAGAAGAT GTCCGACTTC 
CTTAAAGACT GCGCCGAGAG GATCATTATC CTGAAGACGG TTCACCGAAG GATCATCAAC 
AGGTTCCACT CCTTCCTGCT GTACCTCGGC CATCCTCCCT ACAGCGTCAG AGAGATCAGC 
GTCAACAGGT TCAGCAAAAT CCTGAGCGAG TTTGCGCTGG AGTACCGAAC CACCCGCGAG 
CGCGTGCTGC AGCAGAAGCA GAAGCGAGCC GACCACCGCG AACGCAACAA GACCCGAGGA 
AAGATGATCA TGGACGTAAA CGCTCCTCAT TCTGGTCCCG GCGGCTCAAG CAGCAGCAGC 
GGCGCCCCCA GTGGCACCCA GGAGGTGGAG CAGCCTCAGG GGCTGGGCCA CGCCGAGGAC 
GCCGCAGAGC ACGAGCACAT GACAGCAGTG CTGAGGAGCA GCCTGAGCGG CAGCGCCGAT 
AGAGAGGGCG GAGTGCCGGG TCTGCGCACC AGAACCAGGT CCCGGCCTGG ACGAGGAGGG 
CGCAGCATGC AGGCGTGGAC TCCACCCGCA GAGGACGCTC AGACATGTGG AGACGATGCT 
GCAGACGAGA TCATGGAGCG GATCGTCCGG TCTGCCACAC AGGGTCCAGG AACCAGAACA 
CAGCCCAGAG AGCGCAGGAG GTCCAGGGCC AACCGGAAAT CATTGAGACG GACTCTGAAG 
AACGGCCTCA GCCCAGAGGA AGCGCTGGCT TTAGGCCTGA CCGATTCTCC AGAGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023815999.1
CDS	451..5250
Translation	MATLVCRVQFLDDTDPFNSTNFPEPTRPPLYTFREDIPLINQLAGIHRLLKAPHKLDDCTLQLSHNGTYLDLESSLAEQRDELEGFQPDDTGSRGRKHSVILRTQLTVRVHACIEKLYNCSGRDLRRALFSLKQIFQDDKDLVHEFVMAEGLTCLIKVGAEADQNYQNYILRALGQIMLYVDGMNGVISHPETIQWLYTLVGSKFRLVVKTALKLLLVFVEYSESNAALLIQAIASVDTKGGCKPWSNAMEILHEKDGVDTELLVYAMTLINKTLAALPDQDSFYDTVDSLEEQGMEAVTQRHLGRRGTDLDLVEQLNIYEMTLRHEDGDDCQPPSTGRRDRQRASLGGGDKHQGLERRRSRRASLGRSGQASPCSPASPRAGFHPFSGHTVEDLSEREEEEEEEETPVTESDSDEQEGAHQITSSPNWGGKYFSVSDPSVDPCVCSPPRPPPPTAHRTSHRSITSRKEKEQKSRTPSPPAAPPSPSKPPCSPPAHRSLLSTLLARKRSIVTVPATTEVSPPLRGSSRPSPDRLPYLPHSPFHLFSYDLEEEASASAQTKRSTESASPRNGDPASHTSFSSPSTPTSSRPALGGLLSSSYRQHQESLAAERERRRVEREERLLRIEREERNKHSRDYVDKMEEARLAREERYKNVERLAAEEYEKERVRGSRTRPDLNLSFEPSEAWPSSPRSSTPSSFASQEDAEAEVEAVTPVALSETGKATDSSDGGGVEAEDREEEPAAEEEDEHKEEEGEQEEAAAEKEEEEAEEQEPQKQREEEVEEDSGILSDKERQNEEVNEKDNCSASSISSASSTLEREERGTGENGPPGLKEAEVSDSCNKLLNSKLFMLDMLYSQNKKPCEEEEEEEEEVEKEKEKGEGEDRETDQDASGERGDGENKKDDKSDHRGSIRQIAERFGDLIKDAGLPHPHPDDPVNELPPPPAPKKESDTIWDQLMSSPRELRIGDANFTDLTDEDDKDILDAVLMGGCSDLPPPPPPLPFIPRFPPPPPVLGICPLPPPLMGSMRPPPPPSFSSPMPGLAPQEQPLFNKKKKTIRLFWSEVRPTDCQYRDYKRGGDSFWSKLDTVKLDTAKLENLFETKSKEISVTKKTAADGKRQEIIVLDSKRSNAINIGLTVLPPPRTIKTAILNFDEYALNKEGIEKLLTMIPTEEEKQKIQEAQLANPDVPLGSAEQFLLTLSSISELSARLHLWAFKMDQEATEKEVVEPLQDLKEGMEQLEKNKTLRYILSTLLSIGNFLNGTNAKGFELTYLEKVPEVKDTVHKQSLLHHVCTVVVENFPQSTDLYSEIGAITRSAKVDFDQLQENLCQMERRCKASWDHLKVIAKHEMKPQLKQKMSDFLKDCAERIIILKTVHRRIINRFHSFLLYLGHPPYSVREISVNRFSKILSEFALEYRTTRERVLQQKQKRADHRERNKTRGKMIMDVNAPHSGPGGSSSSSGAPSGTQEVEQPQGLGHAEDAAEHEHMTAVLRSSLSGSADREGGVPGLRTRTRSRPGRGGRSMQAWTPPAEDAQTCGDDAADEIMERIVRSATQGPGTRTQPRERRRSRANRKSLRRTLKNGLSPEEALALGLTDSPET

Target ORF information:

RefSeq Version	XM_023960231.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023960231.1

ORF Insert Sequence:

ATGGCCACGC TCGTCTGCAG GGTTCAGTTC TTAGATGACA CCGATCCGTT CAACAGCACC 
AACTTCCCGG AGCCCACGCG CCCGCCGCTC TACACCTTCA GGGAGGACAT CCCCCTCATC 
AACCAGCTCG CGGGCATCCA CCGGCTCCTC AAGGCTCCTC ACAAGCTCGA TGACTGCACA 
CTTCAGCTCT CCCACAATGG GACCTATCTG GATCTGGAGT CCTCCCTGGC CGAGCAAAGG 
GACGAGCTGG AGGGCTTTCA GCCCGACGAC ACTGGGTCCA GGGGGAGGAA GCACAGTGTC 
ATTCTACGGA CTCAGCTGAC GGTGCGCGTG CACGCCTGCA TCGAAAAACT CTACAACTGC 
AGTGGGCGGG ACTTAAGGAG GGCGCTGTTC TCCTTGAAGC AGATTTTTCA GGATGACAAA 
GATCTGGTCC ATGAGTTTGT GATGGCTGAA GGTCTGACGT GTCTCATCAA GGTGGGAGCA 
GAAGCCGACC AGAACTATCA GAACTACATT CTGAGAGCTC TGGGGCAGAT CATGCTGTAC 
GTGGACGGCA TGAACGGAGT CATCAGCCAC CCCGAGACCA TCCAGTGGCT CTACACGCTG 
GTCGGCTCAA AGTTTCGGTT GGTGGTGAAA ACGGCTCTGA AGCTGCTGCT GGTGTTTGTG 
GAGTACTCAG AGTCCAACGC GGCGCTGCTC ATTCAGGCCA TCGCCTCCGT GGACACGAAG 
GGAGGCTGCA AACCGTGGTC CAATGCCATG GAGATCCTAC ATGAGAAGGA TGGGGTGGAC 
ACGGAGTTGC TGGTCTACGC CATGACCCTC ATTAATAAGA CTTTGGCGGC ACTACCGGAT 
CAGGACTCCT TCTATGACAC GGTGGACAGC TTGGAGGAGC AAGGCATGGA GGCGGTCACC 
CAGAGGCACC TGGGGAGGAG AGGCACCGAC CTGGACCTGG TTGAGCAGCT CAACATTTAT 
GAGATGACCC TACGGCACGA AGATGGGGAC GACTGCCAGC CCCCATCCAC AGGTCGCCGG 
GACCGCCAGC GGGCCAGCCT CGGGGGAGGG GACAAACATC AAGGTCTGGA GAGGAGACGG 
AGCCGGCGGG CATCTCTTGG TCGATCGGGT CAGGCTTCTC CGTGCAGCCC AGCGTCACCG 
AGAGCCGGCT TTCATCCCTT CAGCGGACAC ACGGTTGAAG ACCTGAGTGA AAGAGAGGAG 
GAGGAGGAGG AGGAGGAGAC TCCTGTGACT GAGTCAGATT CGGATGAGCA GGAGGGGGCT 
CATCAGATCA CCTCCAGTCC AAACTGGGGG GGCAAATACT TCTCTGTGAG TGACCCATCT 
GTTGACCCCT GTGTTTGTAG CCCCCCCAGA CCCCCTCCAC CCACCGCTCA CAGAACGAGC 
CATCGCTCCA TCACCTCCAG GAAGGAGAAA GAGCAGAAGA GCAGGACCCC TTCGCCCCCA 
GCAGCCCCAC CCTCACCATC TAAACCCCCC TGCAGCCCCC CAGCCCACCG CTCCCTGCTA 
TCTACCTTAC TGGCACGGAA GCGGTCCATC GTCACCGTTC CAGCCACCAC GGAGGTCAGT 
CCTCCTCTGC GCGGCAGCTC CCGCCCCTCC CCGGACCGCC TGCCCTACCT GCCCCACTCC 
CCCTTCCACC TCTTCTCATA CGATCTTGAG GAGGAGGCGT CCGCTTCGGC CCAGACCAAA 
CGCAGCACGG AGTCGGCATC TCCAAGGAAC GGAGATCCTG CGTCTCACAC CTCGTTCAGC 
TCGCCAAGCA CGCCCACCAG CTCCAGACCG GCTCTGGGAG GACTGCTGTC ATCCTCCTAC 
AGGCAGCACC AGGAGTCTCT GGCTGCTGAG CGAGAGCGCC GCCGTGTGGA GCGAGAGGAG 
CGACTGCTCA GGATAGAGAG AGAGGAGAGA AACAAGCACA GCCGCGACTA TGTGGATAAA 
ATGGAAGAGG CCAGACTTGC GCGAGAGGAG AGGTATAAGA ACGTGGAGCG CTTGGCCGCA 
GAGGAGTATG AGAAGGAGCG TGTCCGAGGG TCAAGGACTC GCCCTGACCT CAACCTAAGC 
TTTGAGCCCT CCGAGGCCTG GCCTTCATCG CCGCGCAGCA GCACCCCATC TTCCTTCGCC 
TCGCAGGAGG ACGCAGAGGC AGAGGTGGAG GCGGTGACGC CAGTCGCGCT CTCTGAGACG 
GGGAAAGCCA CAGATTCAAG CGACGGCGGC GGAGTGGAAG CGGAGGACAG AGAGGAAGAG 
CCCGCTGCTG AAGAGGAGGA CGAACACAAA GAAGAGGAGG GAGAACAGGA GGAAGCGGCA 
GCAGAGAAGG AAGAGGAGGA AGCTGAGGAG CAGGAGCCGC AGAAGCAGAG AGAAGAGGAG 
GTGGAGGAGG ACAGTGGCAT CCTGAGTGAT AAGGAGCGAC AGAATGAGGA AGTAAACGAG 
AAAGACAACT GCTCAGCGTC CAGCATCTCC TCTGCCAGCA GCACTTTAGA GCGAGAGGAG 
AGGGGAACCG GGGAGAATGG CCCCCCAGGC CTAAAGGAAG CAGAAGTCAG CGACAGTTGC 
AACAAACTCC TCAACAGCAA ACTCTTCATG CTGGACATGC TCTACTCCCA AAACAAGAAA 
CCATGCGAGG AGGAGGAGGA GGAAGAGGAG GAGGTGGAGA AGGAGAAGGA GAAAGGAGAA 
GGTGAGGACA GAGAGACAGA CCAGGATGCC AGCGGAGAAC GCGGCGACGG GGAAAACAAG 
AAGGACGACA AGTCAGACCA TCGCGGGAGC ATCCGTCAGA TTGCCGAGCG GTTCGGAGAC 
CTGATCAAGG ACGCCGGCTT GCCCCACCCC CACCCGGACG ACCCGGTCAA TGAGCTCCCT 
CCGCCCCCCG CGCCCAAGAA AGAGTCGGAC ACCATCTGGG ACCAGCTCAT GTCCAGCCCC 
CGAGAGCTGC GCATCGGAGA CGCCAACTTC ACCGACCTGA CGGATGAGGA CGACAAGGAC 
ATTCTGGACG CTGTTCTGAT GGGAGGATGC AGTGACCTCC CACCCCCGCC GCCCCCTCTA 
CCCTTCATCC CACGCTTTCC ACCACCTCCA CCAGTGCTAG GCATCTGCCC CTTGCCCCCT 
CCTTTGATGG GCAGCATGAG GCCTCCACCT CCACCGTCCT TTAGCTCTCC GATGCCAGGG 
CTGGCCCCCC AGGAGCAGCC TCTGTTCAAC AAAAAGAAGA AGACCATCCG ACTCTTCTGG 
AGTGAAGTTC GGCCCACAGA CTGTCAGTAC AGGGACTACA AACGAGGGGG AGACTCATTC 
TGGTCCAAAC TGGACACAGT CAAGCTGGAC ACAGCCAAAC TGGAGAACCT GTTTGAGACC 
AAGTCAAAGG AGATATCAGT TACAAAGAAA ACAGCAGCTG ATGGCAAACG TCAGGAGATC 
ATCGTTTTGG ACTCCAAGAG AAGCAACGCC ATCAACATCG GGCTGACGGT CCTGCCGCCG 
CCTCGAACCA TCAAGACAGC CATCCTCAAC TTTGACGAAT ACGCTCTCAA CAAGGAAGGC 
ATAGAGAAAC TCCTGACAAT GATCCCAACG GAGGAGGAGA AGCAGAAGAT CCAAGAGGCC 
CAGTTGGCCA ATCCGGATGT GCCGTTGGGT TCTGCAGAGC AGTTTCTCCT CACTCTGTCC 
TCCATCAGTG AGCTCTCTGC CAGACTCCAC CTCTGGGCCT TTAAGATGGA CCAAGAAGCT 
ACGGAAAAGG AAGTGGTGGA GCCACTGCAA GATCTGAAGG AGGGGATGGA GCAACTGGAG 
AAAAACAAAA CTCTGCGCTA CATCCTCTCC ACCCTGCTCT CCATTGGAAA CTTCCTCAAC 
GGCACCAATG CCAAAGGCTT TGAGCTGACC TACCTGGAGA AGGTTCCAGA AGTGAAGGAC 
ACGGTTCACA AGCAGTCGCT GCTCCACCAT GTCTGCACGG TGGTGGTGGA AAACTTCCCC 
CAGAGCACCG ACCTGTACTC AGAGATCGGA GCGATCACCC GCTCAGCAAA GGTGGATTTC 
GACCAGCTGC AGGAAAACCT GTGCCAGATG GAGCGGCGCT GCAAAGCCTC ATGGGACCAT 
CTGAAAGTCA TTGCCAAGCA TGAGATGAAG CCTCAGCTAA AACAGAAGAT GTCCGACTTC 
CTTAAAGACT GCGCCGAGAG GATCATTATC CTGAAGACGG TTCACCGAAG GATCATCAAC 
AGGTTCCACT CCTTCCTGCT GTACCTCGGC CATCCTCCCT ACAGCGTCAG AGAGATCAGC 
GTCAACAGGT TCAGCAAAAT CCTGAGCGAG TTTGCGCTGG AGTACCGAAC CACCCGCGAG 
CGCGTGCTGC AGCAGAAGCA GAAGCGAGCC GACCACCGCG AACGCAACAA GACCCGAGGA 
AAGATGATCA TGGACGTAAA CGCTCCTCAT TCTGGTCCCG GCGGCTCAAG CAGCAGCAGC 
GGCGCCCCCA GTGGCACCCA GGAGGTGGAG CAGCCTCAGG GGCTGGGCCA CGCCGAGGAC 
GCCGCAGAGC ACGAGCACAT GACAGCAGTG CTGAGGAGCA GCCTGAGCGG CAGCGCCGAT 
AGAGAGGGCG GAGTGCCGGG TCTGCGCACC AGAACCAGGT CCCGGCCTGG ACGAGGAGGG 
CGCAGCATGC AGGCGTGGAC TCCACCCGCA GAGGACGCTC AGACATGTGG AGACGATGCT 
GCAGACGAGA TCATGGAGCG GATCGTCCGG TCTGCCACAC AGGGTCCAGG AACCAGAACA 
CAGCCCAGAG AGCGCAGGAG GTCCAGGGCC AACCGGAAAT CATTGAGACG GACTCTGAAG 
AACGGCCTCA GCCCAGAGGA AGCGCTGGCT TTAGGCCTGA CCGATTCTCC AGAGACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101171180 ( XM_023960232.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023816000.1 Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X3, mRNA.

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CloneID	OJa107317
Clone ID Related Accession (Same CDS sequence)	XM_023960232.1
Accession Version	XM_023960232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	FH1/FH2 domain-containing protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019869.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC TCGTCTGCAG GGTTCAGTTC TTAGATGACA CCGATCCGTT CAACAGCACC 
AACTTCCCGG AGCCCACGCG CCCGCCGCTC TACACCTTCA GGGAGGACAT CCCCCTCATC 
AACCAGCTCG CGGGCATCCA CCGGCTCCTC AAGGCTCCTC ACAAGCTCGA TGACTGCACA 
CTTCAGCTCT CCCACAATGG GACCTATCTG GATCTGGAGT CCTCCCTGGC CGAGCAAAGG 
GACGAGCTGG AGGGCTTTCA GCCCGACGAC ACTGGGTCCA GGGGGAGGAA GCACAGTGTC 
ATTCTACGGA CTCAGCTGAC GGTGCGCGTG CACGCCTGCA TCGAAAAACT CTACAACTGC 
AGTGGGCGGG ACTTAAGGAG GGCGCTGTTC TCCTTGAAGC AGATTTTTCA GGATGACAAA 
GATCTGGTCC ATGAGTTTGT GATGGCTGAA GGTCTGACGT GTCTCATCAA GGTGGGAGCA 
GAAGCCGACC AGAACTATCA GAACTACATT CTGAGAGCTC TGGGGCAGAT CATGCTGTAC 
GTGGACGGCA TGAACGGAGT CATCAGCCAC CCCGAGACCA TCCAGTGGCT CTACACGCTG 
GTCGGCTCAA AGTTTCGGTT GGTGGTGAAA ACGGCTCTGA AGCTGCTGCT GGTGTTTGTG 
GAGTACTCAG AGTCCAACGC GGCGCTGCTC ATTCAGGCCA TCGCCTCCGT GGACACGAAG 
GGAGGCTGCA AACCGTGGTC CAATGCCATG GAGATCCTAC ATGAGAAGGA TGGGGTGGAC 
ACGGAGTTGC TGGTCTACGC CATGACCCTC ATTAATAAGA CTTTGGCGGC ACTACCGGAT 
CAGGACTCCT TCTATGACAC GGTGGACAGC TTGGAGGAGC AAGGCATGGA GGCGGTCACC 
CAGAGGCACC TGGGGAGGAG AGGCACCGAC CTGGACCTGG TTGAGCAGCT CAACATTTAT 
GAGATGACCC TACGGCACGA AGATGGGGAC GACTGCCAGC CCCCATCCAC AGGTCGCCGG 
GACCGCCAGC GGGCCAGCCT CGGGGGAGGG GACAAACATC AAGGTCTGGA GAGGAGACGG 
AGCCGGCGGG CATCTCTTGG TCGATCGGGT CAGGCTTCTC CGTGCAGCCC AGCGTCACCG 
AGAGCCGGCT TTCATCCCTT CAGCGGACAC ACGGTTGAAG ACCTGAGTGA AAGAGAGGAG 
GAGGAGGAGG AGGAGGAGAC TCCTGTGACT GAGTCAGATT CGGATGAGCA GGAGGGGGCT 
CATCAGATCA CCTCCAGCCC CCCCAGACCC CCTCCACCCA CCGCTCACAG AACGAGCCAT 
CGCTCCATCA CCTCCAGGAA GGAGAAAGAG CAGAAGAGCA GGACCCCTTC GCCCCCAGCA 
GCCCCACCCT CACCATCTAA ACCCCCCTGC AGCCCCCCAG CCCACCGCTC CCTGCTATCT 
ACCTTACTGG CACGGAAGCG GTCCATCGTC ACCGTTCCAG CCACCACGGA GGTCAGTCCT 
CCTCTGCGCG GCAGCTCCCG CCCCTCCCCG GACCGCCTGC CCTACCTGCC CCACTCCCCC 
TTCCACCTCT TCTCATACGA TCTTGAGGAG GAGGCGTCCG CTTCGGCCCA GACCAAACGC 
AGCACGGAGT CGGCATCTCC AAGGAACGGA GATCCTGCGT CTCACACCTC GTTCAGCTCG 
CCAAGCACGC CCACCAGCTC CAGACCGGCT CTGGGAGGAC TGCTGTCATC CTCCTACAGG 
CAGCACCAGG AGTCTCTGGC TGCTGAGCGA GAGCGCCGCC GTGTGGAGCG AGAGGAGCGA 
CTGCTCAGGA TAGAGAGAGA GGAGAGAAAC AAGCACAGCC GCGACTATGT GGATAAAATG 
GAAGAGGCCA GACTTGCGCG AGAGGAGAGG TATAAGAACG TGGAGCGCTT GGCCGCAGAG 
GAGTATGAGA AGGAGCGTGT CCGAGGGTCA AGGACTCGCC CTGACCTCAA CCTAAGCTTT 
GAGCCCTCCG AGGCCTGGCC TTCATCGCCG CGCAGCAGCA CCCCATCTTC CTTCGCCTCG 
CAGGAGGACG CAGAGGCAGA GGTGGAGGCG GTGACGCCAG TCGCGCTCTC TGAGACGGGG 
AAAGCCACAG ATTCAAGCGA CGGCGGCGGA GTGGAAGCGG AGGACAGAGA GGAAGAGCCC 
GCTGCTGAAG AGGAGGACGA ACACAAAGAA GAGGAGGGAG AACAGGAGGA AGCGGCAGCA 
GAGAAGGAAG AGGAGGAAGC TGAGGAGCAG GAGCCGCAGA AGCAGAGAGA AGAGGAGGTG 
GAGGAGGACA GTGGCATCCT GAGTGATAAG GAGCGACAGA ATGAGGAAGT AAACGAGAAA 
GACAACTGCT CAGCGTCCAG CATCTCCTCT GCCAGCAGCA CTTTAGAGCG AGAGGAGAGG 
GGAACCGGGG AGAATGGCCC CCCAGGCCTA AAGGAAGCAG AAGTCAGCGA CAGTTGCAAC 
AAACTCCTCA ACAGCAAACT CTTCATGCTG GACATGCTCT ACTCCCAAAA CAAGAAACCA 
TGCGAGGAGG AGGAGGAGGA AGAGGAGGAG GTGGAGAAGG AGAAGGAGAA AGGAGAAGGT 
GAGGACAGAG AGACAGACCA GGATGCCAGC GGAGAACGCG GCGACGGGGA AAACAAGAAG 
GACGACAAGT CAGACCATCG CGGGAGCATC CGTCAGATTG CCGAGCGGTT CGGAGACCTG 
ATCAAGGACG CCGGCTTGCC CCACCCCCAC CCGGACGACC CGGTCAATGA GCTCCCTCCG 
CCCCCCGCGC CCAAGAAAGA GTCGGACACC ATCTGGGACC AGCTCATGTC CAGCCCCCGA 
GAGCTGCGCA TCGGAGACGC CAACTTCACC GACCTGACGG ATGAGGACGA CAAGGACATT 
CTGGACGCTG TTCTGATGGG AGGATGCAGT GACCTCCCAC CCCCGCCGCC CCCTCTACCC 
TTCATCCCAC GCTTTCCACC ACCTCCACCA GTGCTAGGCA TCTGCCCCTT GCCCCCTCCT 
TTGATGGGCA GCATGAGGCC TCCACCTCCA CCGTCCTTTA GCTCTCCGAT GCCAGGGCTG 
GCCCCCCAGG AGCAGCCTCT GTTCAACAAA AAGAAGAAGA CCATCCGACT CTTCTGGAGT 
GAAGTTCGGC CCACAGACTG TCAGTACAGG GACTACAAAC GAGGGGGAGA CTCATTCTGG 
TCCAAACTGG ACACAGTCAA GCTGGACACA GCCAAACTGG AGAACCTGTT TGAGACCAAG 
TCAAAGGAGA TATCAGTTAC AAAGAAAACA GCAGCTGATG GCAAACGTCA GGAGATCATC 
GTTTTGGACT CCAAGAGAAG CAACGCCATC AACATCGGGC TGACGGTCCT GCCGCCGCCT 
CGAACCATCA AGACAGCCAT CCTCAACTTT GACGAATACG CTCTCAACAA GGAAGGCATA 
GAGAAACTCC TGACAATGAT CCCAACGGAG GAGGAGAAGC AGAAGATCCA AGAGGCCCAG 
TTGGCCAATC CGGATGTGCC GTTGGGTTCT GCAGAGCAGT TTCTCCTCAC TCTGTCCTCC 
ATCAGTGAGC TCTCTGCCAG ACTCCACCTC TGGGCCTTTA AGATGGACCA AGAAGCTACG 
GAAAAGGAAG TGGTGGAGCC ACTGCAAGAT CTGAAGGAGG GGATGGAGCA ACTGGAGAAA 
AACAAAACTC TGCGCTACAT CCTCTCCACC CTGCTCTCCA TTGGAAACTT CCTCAACGGC 
ACCAATGCCA AAGGCTTTGA GCTGACCTAC CTGGAGAAGG TTCCAGAAGT GAAGGACACG 
GTTCACAAGC AGTCGCTGCT CCACCATGTC TGCACGGTGG TGGTGGAAAA CTTCCCCCAG 
AGCACCGACC TGTACTCAGA GATCGGAGCG ATCACCCGCT CAGCAAAGGT GGATTTCGAC 
CAGCTGCAGG AAAACCTGTG CCAGATGGAG CGGCGCTGCA AAGCCTCATG GGACCATCTG 
AAAGTCATTG CCAAGCATGA GATGAAGCCT CAGCTAAAAC AGAAGATGTC CGACTTCCTT 
AAAGACTGCG CCGAGAGGAT CATTATCCTG AAGACGGTTC ACCGAAGGAT CATCAACAGG 
TTCCACTCCT TCCTGCTGTA CCTCGGCCAT CCTCCCTACA GCGTCAGAGA GATCAGCGTC 
AACAGGTTCA GCAAAATCCT GAGCGAGTTT GCGCTGGAGT ACCGAACCAC CCGCGAGCGC 
GTGCTGCAGC AGAAGCAGAA GCGAGCCGAC CACCGCGAAC GCAACAAGAC CCGAGGAAAG 
ATGATCATGG ACGTAAACGC TCCTTCTGAT GATGAAGAAG AGCGTGAGCA TTCTGGTCCC 
GGCGGCTCAA GCAGCAGCAG CGGCGCCCCC AGTGGCACCC AGGAGGTGGA GCAGCCTCAG 
GGGCTGGGCC ACGCCGAGGA CGCCGCAGAG CACGAGCACA TGACAGCAGT GCTGAGGAGC 
AGCCTGAGCG GCAGCGCCGA TAGAGAGGGC GGAGTGCCGG GTCTGCGCAC CAGAACCAGG 
TCCCGGCCTG GACGAGGAGG GCGCAGCATG CAGGCGTGGA CTCCACCCGC AGAGGACGCT 
CAGACATGTG GAGACGATGC TGCAGACGAG ATCATGGAGC GGATCGTCCG GTCTGCCACA 
CAGGGTCCAG GAACCAGAAC ACAGCCCAGA GAGCGCAGGA GGTCCAGGGC CAACCGGAAA 
TCATTGAGAC GGACTCTGAA GAACGGCCTC AGCCCAGAGG AAGCGCTGGC TTTAGGCCTG 
ACCGATTCTC CAGAGACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023816000.1
CDS	449..5209
Translation	MATLVCRVQFLDDTDPFNSTNFPEPTRPPLYTFREDIPLINQLAGIHRLLKAPHKLDDCTLQLSHNGTYLDLESSLAEQRDELEGFQPDDTGSRGRKHSVILRTQLTVRVHACIEKLYNCSGRDLRRALFSLKQIFQDDKDLVHEFVMAEGLTCLIKVGAEADQNYQNYILRALGQIMLYVDGMNGVISHPETIQWLYTLVGSKFRLVVKTALKLLLVFVEYSESNAALLIQAIASVDTKGGCKPWSNAMEILHEKDGVDTELLVYAMTLINKTLAALPDQDSFYDTVDSLEEQGMEAVTQRHLGRRGTDLDLVEQLNIYEMTLRHEDGDDCQPPSTGRRDRQRASLGGGDKHQGLERRRSRRASLGRSGQASPCSPASPRAGFHPFSGHTVEDLSEREEEEEEEETPVTESDSDEQEGAHQITSSPPRPPPPTAHRTSHRSITSRKEKEQKSRTPSPPAAPPSPSKPPCSPPAHRSLLSTLLARKRSIVTVPATTEVSPPLRGSSRPSPDRLPYLPHSPFHLFSYDLEEEASASAQTKRSTESASPRNGDPASHTSFSSPSTPTSSRPALGGLLSSSYRQHQESLAAERERRRVEREERLLRIEREERNKHSRDYVDKMEEARLAREERYKNVERLAAEEYEKERVRGSRTRPDLNLSFEPSEAWPSSPRSSTPSSFASQEDAEAEVEAVTPVALSETGKATDSSDGGGVEAEDREEEPAAEEEDEHKEEEGEQEEAAAEKEEEEAEEQEPQKQREEEVEEDSGILSDKERQNEEVNEKDNCSASSISSASSTLEREERGTGENGPPGLKEAEVSDSCNKLLNSKLFMLDMLYSQNKKPCEEEEEEEEEVEKEKEKGEGEDRETDQDASGERGDGENKKDDKSDHRGSIRQIAERFGDLIKDAGLPHPHPDDPVNELPPPPAPKKESDTIWDQLMSSPRELRIGDANFTDLTDEDDKDILDAVLMGGCSDLPPPPPPLPFIPRFPPPPPVLGICPLPPPLMGSMRPPPPPSFSSPMPGLAPQEQPLFNKKKKTIRLFWSEVRPTDCQYRDYKRGGDSFWSKLDTVKLDTAKLENLFETKSKEISVTKKTAADGKRQEIIVLDSKRSNAINIGLTVLPPPRTIKTAILNFDEYALNKEGIEKLLTMIPTEEEKQKIQEAQLANPDVPLGSAEQFLLTLSSISELSARLHLWAFKMDQEATEKEVVEPLQDLKEGMEQLEKNKTLRYILSTLLSIGNFLNGTNAKGFELTYLEKVPEVKDTVHKQSLLHHVCTVVVENFPQSTDLYSEIGAITRSAKVDFDQLQENLCQMERRCKASWDHLKVIAKHEMKPQLKQKMSDFLKDCAERIIILKTVHRRIINRFHSFLLYLGHPPYSVREISVNRFSKILSEFALEYRTTRERVLQQKQKRADHRERNKTRGKMIMDVNAPSDDEEEREHSGPGGSSSSSGAPSGTQEVEQPQGLGHAEDAAEHEHMTAVLRSSLSGSADREGGVPGLRTRTRSRPGRGGRSMQAWTPPAEDAQTCGDDAADEIMERIVRSATQGPGTRTQPRERRRSRANRKSLRRTLKNGLSPEEALALGLTDSPET

Target ORF information:

RefSeq Version	XM_023960232.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023960232.1

ORF Insert Sequence:

ATGGCCACGC TCGTCTGCAG GGTTCAGTTC TTAGATGACA CCGATCCGTT CAACAGCACC 
AACTTCCCGG AGCCCACGCG CCCGCCGCTC TACACCTTCA GGGAGGACAT CCCCCTCATC 
AACCAGCTCG CGGGCATCCA CCGGCTCCTC AAGGCTCCTC ACAAGCTCGA TGACTGCACA 
CTTCAGCTCT CCCACAATGG GACCTATCTG GATCTGGAGT CCTCCCTGGC CGAGCAAAGG 
GACGAGCTGG AGGGCTTTCA GCCCGACGAC ACTGGGTCCA GGGGGAGGAA GCACAGTGTC 
ATTCTACGGA CTCAGCTGAC GGTGCGCGTG CACGCCTGCA TCGAAAAACT CTACAACTGC 
AGTGGGCGGG ACTTAAGGAG GGCGCTGTTC TCCTTGAAGC AGATTTTTCA GGATGACAAA 
GATCTGGTCC ATGAGTTTGT GATGGCTGAA GGTCTGACGT GTCTCATCAA GGTGGGAGCA 
GAAGCCGACC AGAACTATCA GAACTACATT CTGAGAGCTC TGGGGCAGAT CATGCTGTAC 
GTGGACGGCA TGAACGGAGT CATCAGCCAC CCCGAGACCA TCCAGTGGCT CTACACGCTG 
GTCGGCTCAA AGTTTCGGTT GGTGGTGAAA ACGGCTCTGA AGCTGCTGCT GGTGTTTGTG 
GAGTACTCAG AGTCCAACGC GGCGCTGCTC ATTCAGGCCA TCGCCTCCGT GGACACGAAG 
GGAGGCTGCA AACCGTGGTC CAATGCCATG GAGATCCTAC ATGAGAAGGA TGGGGTGGAC 
ACGGAGTTGC TGGTCTACGC CATGACCCTC ATTAATAAGA CTTTGGCGGC ACTACCGGAT 
CAGGACTCCT TCTATGACAC GGTGGACAGC TTGGAGGAGC AAGGCATGGA GGCGGTCACC 
CAGAGGCACC TGGGGAGGAG AGGCACCGAC CTGGACCTGG TTGAGCAGCT CAACATTTAT 
GAGATGACCC TACGGCACGA AGATGGGGAC GACTGCCAGC CCCCATCCAC AGGTCGCCGG 
GACCGCCAGC GGGCCAGCCT CGGGGGAGGG GACAAACATC AAGGTCTGGA GAGGAGACGG 
AGCCGGCGGG CATCTCTTGG TCGATCGGGT CAGGCTTCTC CGTGCAGCCC AGCGTCACCG 
AGAGCCGGCT TTCATCCCTT CAGCGGACAC ACGGTTGAAG ACCTGAGTGA AAGAGAGGAG 
GAGGAGGAGG AGGAGGAGAC TCCTGTGACT GAGTCAGATT CGGATGAGCA GGAGGGGGCT 
CATCAGATCA CCTCCAGCCC CCCCAGACCC CCTCCACCCA CCGCTCACAG AACGAGCCAT 
CGCTCCATCA CCTCCAGGAA GGAGAAAGAG CAGAAGAGCA GGACCCCTTC GCCCCCAGCA 
GCCCCACCCT CACCATCTAA ACCCCCCTGC AGCCCCCCAG CCCACCGCTC CCTGCTATCT 
ACCTTACTGG CACGGAAGCG GTCCATCGTC ACCGTTCCAG CCACCACGGA GGTCAGTCCT 
CCTCTGCGCG GCAGCTCCCG CCCCTCCCCG GACCGCCTGC CCTACCTGCC CCACTCCCCC 
TTCCACCTCT TCTCATACGA TCTTGAGGAG GAGGCGTCCG CTTCGGCCCA GACCAAACGC 
AGCACGGAGT CGGCATCTCC AAGGAACGGA GATCCTGCGT CTCACACCTC GTTCAGCTCG 
CCAAGCACGC CCACCAGCTC CAGACCGGCT CTGGGAGGAC TGCTGTCATC CTCCTACAGG 
CAGCACCAGG AGTCTCTGGC TGCTGAGCGA GAGCGCCGCC GTGTGGAGCG AGAGGAGCGA 
CTGCTCAGGA TAGAGAGAGA GGAGAGAAAC AAGCACAGCC GCGACTATGT GGATAAAATG 
GAAGAGGCCA GACTTGCGCG AGAGGAGAGG TATAAGAACG TGGAGCGCTT GGCCGCAGAG 
GAGTATGAGA AGGAGCGTGT CCGAGGGTCA AGGACTCGCC CTGACCTCAA CCTAAGCTTT 
GAGCCCTCCG AGGCCTGGCC TTCATCGCCG CGCAGCAGCA CCCCATCTTC CTTCGCCTCG 
CAGGAGGACG CAGAGGCAGA GGTGGAGGCG GTGACGCCAG TCGCGCTCTC TGAGACGGGG 
AAAGCCACAG ATTCAAGCGA CGGCGGCGGA GTGGAAGCGG AGGACAGAGA GGAAGAGCCC 
GCTGCTGAAG AGGAGGACGA ACACAAAGAA GAGGAGGGAG AACAGGAGGA AGCGGCAGCA 
GAGAAGGAAG AGGAGGAAGC TGAGGAGCAG GAGCCGCAGA AGCAGAGAGA AGAGGAGGTG 
GAGGAGGACA GTGGCATCCT GAGTGATAAG GAGCGACAGA ATGAGGAAGT AAACGAGAAA 
GACAACTGCT CAGCGTCCAG CATCTCCTCT GCCAGCAGCA CTTTAGAGCG AGAGGAGAGG 
GGAACCGGGG AGAATGGCCC CCCAGGCCTA AAGGAAGCAG AAGTCAGCGA CAGTTGCAAC 
AAACTCCTCA ACAGCAAACT CTTCATGCTG GACATGCTCT ACTCCCAAAA CAAGAAACCA 
TGCGAGGAGG AGGAGGAGGA AGAGGAGGAG GTGGAGAAGG AGAAGGAGAA AGGAGAAGGT 
GAGGACAGAG AGACAGACCA GGATGCCAGC GGAGAACGCG GCGACGGGGA AAACAAGAAG 
GACGACAAGT CAGACCATCG CGGGAGCATC CGTCAGATTG CCGAGCGGTT CGGAGACCTG 
ATCAAGGACG CCGGCTTGCC CCACCCCCAC CCGGACGACC CGGTCAATGA GCTCCCTCCG 
CCCCCCGCGC CCAAGAAAGA GTCGGACACC ATCTGGGACC AGCTCATGTC CAGCCCCCGA 
GAGCTGCGCA TCGGAGACGC CAACTTCACC GACCTGACGG ATGAGGACGA CAAGGACATT 
CTGGACGCTG TTCTGATGGG AGGATGCAGT GACCTCCCAC CCCCGCCGCC CCCTCTACCC 
TTCATCCCAC GCTTTCCACC ACCTCCACCA GTGCTAGGCA TCTGCCCCTT GCCCCCTCCT 
TTGATGGGCA GCATGAGGCC TCCACCTCCA CCGTCCTTTA GCTCTCCGAT GCCAGGGCTG 
GCCCCCCAGG AGCAGCCTCT GTTCAACAAA AAGAAGAAGA CCATCCGACT CTTCTGGAGT 
GAAGTTCGGC CCACAGACTG TCAGTACAGG GACTACAAAC GAGGGGGAGA CTCATTCTGG 
TCCAAACTGG ACACAGTCAA GCTGGACACA GCCAAACTGG AGAACCTGTT TGAGACCAAG 
TCAAAGGAGA TATCAGTTAC AAAGAAAACA GCAGCTGATG GCAAACGTCA GGAGATCATC 
GTTTTGGACT CCAAGAGAAG CAACGCCATC AACATCGGGC TGACGGTCCT GCCGCCGCCT 
CGAACCATCA AGACAGCCAT CCTCAACTTT GACGAATACG CTCTCAACAA GGAAGGCATA 
GAGAAACTCC TGACAATGAT CCCAACGGAG GAGGAGAAGC AGAAGATCCA AGAGGCCCAG 
TTGGCCAATC CGGATGTGCC GTTGGGTTCT GCAGAGCAGT TTCTCCTCAC TCTGTCCTCC 
ATCAGTGAGC TCTCTGCCAG ACTCCACCTC TGGGCCTTTA AGATGGACCA AGAAGCTACG 
GAAAAGGAAG TGGTGGAGCC ACTGCAAGAT CTGAAGGAGG GGATGGAGCA ACTGGAGAAA 
AACAAAACTC TGCGCTACAT CCTCTCCACC CTGCTCTCCA TTGGAAACTT CCTCAACGGC 
ACCAATGCCA AAGGCTTTGA GCTGACCTAC CTGGAGAAGG TTCCAGAAGT GAAGGACACG 
GTTCACAAGC AGTCGCTGCT CCACCATGTC TGCACGGTGG TGGTGGAAAA CTTCCCCCAG 
AGCACCGACC TGTACTCAGA GATCGGAGCG ATCACCCGCT CAGCAAAGGT GGATTTCGAC 
CAGCTGCAGG AAAACCTGTG CCAGATGGAG CGGCGCTGCA AAGCCTCATG GGACCATCTG 
AAAGTCATTG CCAAGCATGA GATGAAGCCT CAGCTAAAAC AGAAGATGTC CGACTTCCTT 
AAAGACTGCG CCGAGAGGAT CATTATCCTG AAGACGGTTC ACCGAAGGAT CATCAACAGG 
TTCCACTCCT TCCTGCTGTA CCTCGGCCAT CCTCCCTACA GCGTCAGAGA GATCAGCGTC 
AACAGGTTCA GCAAAATCCT GAGCGAGTTT GCGCTGGAGT ACCGAACCAC CCGCGAGCGC 
GTGCTGCAGC AGAAGCAGAA GCGAGCCGAC CACCGCGAAC GCAACAAGAC CCGAGGAAAG 
ATGATCATGG ACGTAAACGC TCCTTCTGAT GATGAAGAAG AGCGTGAGCA TTCTGGTCCC 
GGCGGCTCAA GCAGCAGCAG CGGCGCCCCC AGTGGCACCC AGGAGGTGGA GCAGCCTCAG 
GGGCTGGGCC ACGCCGAGGA CGCCGCAGAG CACGAGCACA TGACAGCAGT GCTGAGGAGC 
AGCCTGAGCG GCAGCGCCGA TAGAGAGGGC GGAGTGCCGG GTCTGCGCAC CAGAACCAGG 
TCCCGGCCTG GACGAGGAGG GCGCAGCATG CAGGCGTGGA CTCCACCCGC AGAGGACGCT 
CAGACATGTG GAGACGATGC TGCAGACGAG ATCATGGAGC GGATCGTCCG GTCTGCCACA 
CAGGGTCCAG GAACCAGAAC ACAGCCCAGA GAGCGCAGGA GGTCCAGGGC CAACCGGAAA 
TCATTGAGAC GGACTCTGAA GAACGGCCTC AGCCCAGAGG AAGCGCTGGC TTTAGGCCTG 
ACCGATTCTC CAGAGACGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101171180 ( XM_023960233.1 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_023816001.1 Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X4, mRNA.

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CloneID	OJa107318
Clone ID Related Accession (Same CDS sequence)	XM_023960233.1
Accession Version	XM_023960233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	FH1/FH2 domain-containing protein 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019869.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACGC TCGTCTGCAG GGTTCAGTTC TTAGATGACA CCGATCCGTT CAACAGCACC 
AACTTCCCGG AGCCCACGCG CCCGCCGCTC TACACCTTCA GGGAGGACAT CCCCCTCATC 
AACCAGCTCG CGGGCATCCA CCGGCTCCTC AAGGCTCCTC ACAAGCTCGA TGACTGCACA 
CTTCAGCTCT CCCACAATGG GACCTATCTG GATCTGGAGT CCTCCCTGGC CGAGCAAAGG 
GACGAGCTGG AGGGCTTTCA GCCCGACGAC ACTGGGTCCA GGGGGAGGAA GCACAGTGTC 
ATTCTACGGA CTCAGCTGAC GGTGCGCGTG CACGCCTGCA TCGAAAAACT CTACAACTGC 
AGTGGGCGGG ACTTAAGGAG GGCGCTGTTC TCCTTGAAGC AGATTTTTCA GGATGACAAA 
GATCTGGTCC ATGAGTTTGT GATGGCTGAA GGTCTGACGT GTCTCATCAA GGTGGGAGCA 
GAAGCCGACC AGAACTATCA GAACTACATT CTGAGAGCTC TGGGGCAGAT CATGCTGTAC 
GTGGACGGCA TGAACGGAGT CATCAGCCAC CCCGAGACCA TCCAGTGGCT CTACACGCTG 
GTCGGCTCAA AGTTTCGGTT GGTGGTGAAA ACGGCTCTGA AGCTGCTGCT GGTGTTTGTG 
GAGTACTCAG AGTCCAACGC GGCGCTGCTC ATTCAGGCCA TCGCCTCCGT GGACACGAAG 
GGAGGCTGCA AACCGTGGTC CAATGCCATG GAGATCCTAC ATGAGAAGGA TGGGGTGGAC 
ACGGAGTTGC TGGTCTACGC CATGACCCTC ATTAATAAGA CTTTGGCGGC ACTACCGGAT 
CAGGACTCCT TCTATGACAC GGTGGACAGC TTGGAGGAGC AAGGCATGGA GGCGGTCACC 
CAGAGGCACC TGGGGAGGAG AGGCACCGAC CTGGACCTGG TTGAGCAGCT CAACATTTAT 
GAGATGACCC TACGGCACGA AGATGGGGAC GACTGCCAGC CCCCATCCAC AGGTCGCCGG 
GACCGCCAGC GGGCCAGCCT CGGGGGAGGG GACAAACATC AAGGTCTGGA GAGGAGACGG 
AGCCGGCGGG CATCTCTTGG TCGATCGGGT CAGGCTTCTC CGTGCAGCCC AGCGTCACCG 
AGAGCCGGCT TTCATCCCTT CAGCGGACAC ACGGTTGAAG ACCTGAGTGA AAGCCCCCCC 
AGACCCCCTC CACCCACCGC TCACAGAACG AGCCATCGCT CCATCACCTC CAGGAAGGAG 
AAAGAGCAGA AGAGCAGGAC CCCTTCGCCC CCAGCAGCCC CACCCTCACC ATCTAAACCC 
CCCTGCAGCC CCCCAGCCCA CCGCTCCCTG CTATCTACCT TACTGGCACG GAAGCGGTCC 
ATCGTCACCG TTCCAGCCAC CACGGAGGTC AGTCCTCCTC TGCGCGGCAG CTCCCGCCCC 
TCCCCGGACC GCCTGCCCTA CCTGCCCCAC TCCCCCTTCC ACCTCTTCTC ATACGATCTT 
GAGGAGGAGG CGTCCGCTTC GGCCCAGACC AAACGCAGCA CGGAGTCGGC ATCTCCAAGG 
AACGGAGATC CTGCGTCTCA CACCTCGTTC AGCTCGCCAA GCACGCCCAC CAGCTCCAGA 
CCGGCTCTGG GAGGACTGCT GTCATCCTCC TACAGGCAGC ACCAGGAGTC TCTGGCTGCT 
GAGCGAGAGC GCCGCCGTGT GGAGCGAGAG GAGCGACTGC TCAGGATAGA GAGAGAGGAG 
AGAAACAAGC ACAGCCGCGA CTATGTGGAT AAAATGGAAG AGGCCAGACT TGCGCGAGAG 
GAGAGGTATA AGAACGTGGA GCGCTTGGCC GCAGAGGAGT ATGAGAAGGA GCGTGTCCGA 
GGGTCAAGGA CTCGCCCTGA CCTCAACCTA AGCTTTGAGC CCTCCGAGGC CTGGCCTTCA 
TCGCCGCGCA GCAGCACCCC ATCTTCCTTC GCCTCGCAGG AGGACGCAGA GGCAGAGGTG 
GAGGCGGTGA CGCCAGTCGC GCTCTCTGAG ACGGGGAAAG CCACAGATTC AAGCGACGGC 
GGCGGAGTGG AAGCGGAGGA CAGAGAGGAA GAGCCCGCTG CTGAAGAGGA GGACGAACAC 
AAAGAAGAGG AGGGAGAACA GGAGGAAGCG GCAGCAGAGA AGGAAGAGGA GGAAGCTGAG 
GAGCAGGAGC CGCAGAAGCA GAGAGAAGAG GAGGTGGAGG AGGACAGTGG CATCCTGAGT 
GATAAGGAGC GACAGAATGA GGAAGTAAAC GAGAAAGACA ACTGCTCAGC GTCCAGCATC 
TCCTCTGCCA GCAGCACTTT AGAGCGAGAG GAGAGGGGAA CCGGGGAGAA TGGCCCCCCA 
GGCCTAAAGG AAGCAGAAGT CAGCGACAGT TGCAACAAAC TCCTCAACAG CAAACTCTTC 
ATGCTGGACA TGCTCTACTC CCAAAACAAG AAACCATGCG AGGAGGAGGA GGAGGAAGAG 
GAGGAGGTGG AGAAGGAGAA GGAGAAAGGA GAAGGTGAGG ACAGAGAGAC AGACCAGGAT 
GCCAGCGGAG AACGCGGCGA CGGGGAAAAC AAGAAGGACG ACAAGTCAGA CCATCGCGGG 
AGCATCCGTC AGATTGCCGA GCGGTTCGGA GACCTGATCA AGGACGCCGG CTTGCCCCAC 
CCCCACCCGG ACGACCCGGT CAATGAGCTC CCTCCGCCCC CCGCGCCCAA GAAAGAGTCG 
GACACCATCT GGGACCAGCT CATGTCCAGC CCCCGAGAGC TGCGCATCGG AGACGCCAAC 
TTCACCGACC TGACGGATGA GGACGACAAG GACATTCTGG ACGCTGTTCT GATGGGAGGA 
TGCAGTGACC TCCCACCCCC GCCGCCCCCT CTACCCTTCA TCCCACGCTT TCCACCACCT 
CCACCAGTGC TAGGCATCTG CCCCTTGCCC CCTCCTTTGA TGGGCAGCAT GAGGCCTCCA 
CCTCCACCGT CCTTTAGCTC TCCGATGCCA GGGCTGGCCC CCCAGGAGCA GCCTCTGTTC 
AACAAAAAGA AGAAGACCAT CCGACTCTTC TGGAGTGAAG TTCGGCCCAC AGACTGTCAG 
TACAGGGACT ACAAACGAGG GGGAGACTCA TTCTGGTCCA AACTGGACAC AGTCAAGCTG 
GACACAGCCA AACTGGAGAA CCTGTTTGAG ACCAAGTCAA AGGAGATATC AGTTACAAAG 
AAAACAGCAG CTGATGGCAA ACGTCAGGAG ATCATCGTTT TGGACTCCAA GAGAAGCAAC 
GCCATCAACA TCGGGCTGAC GGTCCTGCCG CCGCCTCGAA CCATCAAGAC AGCCATCCTC 
AACTTTGACG AATACGCTCT CAACAAGGAA GGCATAGAGA AACTCCTGAC AATGATCCCA 
ACGGAGGAGG AGAAGCAGAA GATCCAAGAG GCCCAGTTGG CCAATCCGGA TGTGCCGTTG 
GGTTCTGCAG AGCAGTTTCT CCTCACTCTG TCCTCCATCA GTGAGCTCTC TGCCAGACTC 
CACCTCTGGG CCTTTAAGAT GGACCAAGAA GCTACGGAAA AGGAAGTGGT GGAGCCACTG 
CAAGATCTGA AGGAGGGGAT GGAGCAACTG GAGAAAAACA AAACTCTGCG CTACATCCTC 
TCCACCCTGC TCTCCATTGG AAACTTCCTC AACGGCACCA ATGCCAAAGG CTTTGAGCTG 
ACCTACCTGG AGAAGGTTCC AGAAGTGAAG GACACGGTTC ACAAGCAGTC GCTGCTCCAC 
CATGTCTGCA CGGTGGTGGT GGAAAACTTC CCCCAGAGCA CCGACCTGTA CTCAGAGATC 
GGAGCGATCA CCCGCTCAGC AAAGGTGGAT TTCGACCAGC TGCAGGAAAA CCTGTGCCAG 
ATGGAGCGGC GCTGCAAAGC CTCATGGGAC CATCTGAAAG TCATTGCCAA GCATGAGATG 
AAGCCTCAGC TAAAACAGAA GATGTCCGAC TTCCTTAAAG ACTGCGCCGA GAGGATCATT 
ATCCTGAAGA CGGTTCACCG AAGGATCATC AACAGGTTCC ACTCCTTCCT GCTGTACCTC 
GGCCATCCTC CCTACAGCGT CAGAGAGATC AGCGTCAACA GGTTCAGCAA AATCCTGAGC 
GAGTTTGCGC TGGAGTACCG AACCACCCGC GAGCGCGTGC TGCAGCAGAA GCAGAAGCGA 
GCCGACCACC GCGAACGCAA CAAGACCCGA GGAAAGATGA TCATGGACGT AAACGCTCCT 
TCTGATGATG AAGAAGAGCG TGAGCATTCT GGTCCCGGCG GCTCAAGCAG CAGCAGCGGC 
GCCCCCAGTG GCACCCAGGA GGTGGAGCAG CCTCAGGGGC TGGGCCACGC CGAGGACGCC 
GCAGAGCACG AGCACATGAC AGCAGTGCTG AGGAGCAGCC TGAGCGGCAG CGCCGATAGA 
GAGGGCGGAG TGCCGGGTCT GCGCACCAGA ACCAGGTCCC GGCCTGGACG AGGAGGGCGC 
AGCATGCAGG CGTGGACTCC ACCCGCAGAG GACGCTCAGA CATGTGGAGA CGATGCTGCA 
GACGAGATCA TGGAGCGGAT CGTCCGGTCT GCCACACAGG GTCCAGGAAC CAGAACACAG 
CCCAGAGAGC GCAGGAGGTC CAGGGCCAAC CGGAAATCAT TGAGACGGAC TCTGAAGAAC 
GGCCTCAGCC CAGAGGAAGC GCTGGCTTTA GGCCTGACCG ATTCTCCAGA GACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023816001.1
CDS	448..5124
Translation	MATLVCRVQFLDDTDPFNSTNFPEPTRPPLYTFREDIPLINQLAGIHRLLKAPHKLDDCTLQLSHNGTYLDLESSLAEQRDELEGFQPDDTGSRGRKHSVILRTQLTVRVHACIEKLYNCSGRDLRRALFSLKQIFQDDKDLVHEFVMAEGLTCLIKVGAEADQNYQNYILRALGQIMLYVDGMNGVISHPETIQWLYTLVGSKFRLVVKTALKLLLVFVEYSESNAALLIQAIASVDTKGGCKPWSNAMEILHEKDGVDTELLVYAMTLINKTLAALPDQDSFYDTVDSLEEQGMEAVTQRHLGRRGTDLDLVEQLNIYEMTLRHEDGDDCQPPSTGRRDRQRASLGGGDKHQGLERRRSRRASLGRSGQASPCSPASPRAGFHPFSGHTVEDLSESPPRPPPPTAHRTSHRSITSRKEKEQKSRTPSPPAAPPSPSKPPCSPPAHRSLLSTLLARKRSIVTVPATTEVSPPLRGSSRPSPDRLPYLPHSPFHLFSYDLEEEASASAQTKRSTESASPRNGDPASHTSFSSPSTPTSSRPALGGLLSSSYRQHQESLAAERERRRVEREERLLRIEREERNKHSRDYVDKMEEARLAREERYKNVERLAAEEYEKERVRGSRTRPDLNLSFEPSEAWPSSPRSSTPSSFASQEDAEAEVEAVTPVALSETGKATDSSDGGGVEAEDREEEPAAEEEDEHKEEEGEQEEAAAEKEEEEAEEQEPQKQREEEVEEDSGILSDKERQNEEVNEKDNCSASSISSASSTLEREERGTGENGPPGLKEAEVSDSCNKLLNSKLFMLDMLYSQNKKPCEEEEEEEEEVEKEKEKGEGEDRETDQDASGERGDGENKKDDKSDHRGSIRQIAERFGDLIKDAGLPHPHPDDPVNELPPPPAPKKESDTIWDQLMSSPRELRIGDANFTDLTDEDDKDILDAVLMGGCSDLPPPPPPLPFIPRFPPPPPVLGICPLPPPLMGSMRPPPPPSFSSPMPGLAPQEQPLFNKKKKTIRLFWSEVRPTDCQYRDYKRGGDSFWSKLDTVKLDTAKLENLFETKSKEISVTKKTAADGKRQEIIVLDSKRSNAINIGLTVLPPPRTIKTAILNFDEYALNKEGIEKLLTMIPTEEEKQKIQEAQLANPDVPLGSAEQFLLTLSSISELSARLHLWAFKMDQEATEKEVVEPLQDLKEGMEQLEKNKTLRYILSTLLSIGNFLNGTNAKGFELTYLEKVPEVKDTVHKQSLLHHVCTVVVENFPQSTDLYSEIGAITRSAKVDFDQLQENLCQMERRCKASWDHLKVIAKHEMKPQLKQKMSDFLKDCAERIIILKTVHRRIINRFHSFLLYLGHPPYSVREISVNRFSKILSEFALEYRTTRERVLQQKQKRADHRERNKTRGKMIMDVNAPSDDEEEREHSGPGGSSSSSGAPSGTQEVEQPQGLGHAEDAAEHEHMTAVLRSSLSGSADREGGVPGLRTRTRSRPGRGGRSMQAWTPPAEDAQTCGDDAADEIMERIVRSATQGPGTRTQPRERRRSRANRKSLRRTLKNGLSPEEALALGLTDSPET

Target ORF information:

RefSeq Version	XM_023960233.1
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes FH1/FH2 domain-containing protein 3 (LOC101171180), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023960233.1

ORF Insert Sequence:

ATGGCCACGC TCGTCTGCAG GGTTCAGTTC TTAGATGACA CCGATCCGTT CAACAGCACC 
AACTTCCCGG AGCCCACGCG CCCGCCGCTC TACACCTTCA GGGAGGACAT CCCCCTCATC 
AACCAGCTCG CGGGCATCCA CCGGCTCCTC AAGGCTCCTC ACAAGCTCGA TGACTGCACA 
CTTCAGCTCT CCCACAATGG GACCTATCTG GATCTGGAGT CCTCCCTGGC CGAGCAAAGG 
GACGAGCTGG AGGGCTTTCA GCCCGACGAC ACTGGGTCCA GGGGGAGGAA GCACAGTGTC 
ATTCTACGGA CTCAGCTGAC GGTGCGCGTG CACGCCTGCA TCGAAAAACT CTACAACTGC 
AGTGGGCGGG ACTTAAGGAG GGCGCTGTTC TCCTTGAAGC AGATTTTTCA GGATGACAAA 
GATCTGGTCC ATGAGTTTGT GATGGCTGAA GGTCTGACGT GTCTCATCAA GGTGGGAGCA 
GAAGCCGACC AGAACTATCA GAACTACATT CTGAGAGCTC TGGGGCAGAT CATGCTGTAC 
GTGGACGGCA TGAACGGAGT CATCAGCCAC CCCGAGACCA TCCAGTGGCT CTACACGCTG 
GTCGGCTCAA AGTTTCGGTT GGTGGTGAAA ACGGCTCTGA AGCTGCTGCT GGTGTTTGTG 
GAGTACTCAG AGTCCAACGC GGCGCTGCTC ATTCAGGCCA TCGCCTCCGT GGACACGAAG 
GGAGGCTGCA AACCGTGGTC CAATGCCATG GAGATCCTAC ATGAGAAGGA TGGGGTGGAC 
ACGGAGTTGC TGGTCTACGC CATGACCCTC ATTAATAAGA CTTTGGCGGC ACTACCGGAT 
CAGGACTCCT TCTATGACAC GGTGGACAGC TTGGAGGAGC AAGGCATGGA GGCGGTCACC 
CAGAGGCACC TGGGGAGGAG AGGCACCGAC CTGGACCTGG TTGAGCAGCT CAACATTTAT 
GAGATGACCC TACGGCACGA AGATGGGGAC GACTGCCAGC CCCCATCCAC AGGTCGCCGG 
GACCGCCAGC GGGCCAGCCT CGGGGGAGGG GACAAACATC AAGGTCTGGA GAGGAGACGG 
AGCCGGCGGG CATCTCTTGG TCGATCGGGT CAGGCTTCTC CGTGCAGCCC AGCGTCACCG 
AGAGCCGGCT TTCATCCCTT CAGCGGACAC ACGGTTGAAG ACCTGAGTGA AAGCCCCCCC 
AGACCCCCTC CACCCACCGC TCACAGAACG AGCCATCGCT CCATCACCTC CAGGAAGGAG 
AAAGAGCAGA AGAGCAGGAC CCCTTCGCCC CCAGCAGCCC CACCCTCACC ATCTAAACCC 
CCCTGCAGCC CCCCAGCCCA CCGCTCCCTG CTATCTACCT TACTGGCACG GAAGCGGTCC 
ATCGTCACCG TTCCAGCCAC CACGGAGGTC AGTCCTCCTC TGCGCGGCAG CTCCCGCCCC 
TCCCCGGACC GCCTGCCCTA CCTGCCCCAC TCCCCCTTCC ACCTCTTCTC ATACGATCTT 
GAGGAGGAGG CGTCCGCTTC GGCCCAGACC AAACGCAGCA CGGAGTCGGC ATCTCCAAGG 
AACGGAGATC CTGCGTCTCA CACCTCGTTC AGCTCGCCAA GCACGCCCAC CAGCTCCAGA 
CCGGCTCTGG GAGGACTGCT GTCATCCTCC TACAGGCAGC ACCAGGAGTC TCTGGCTGCT 
GAGCGAGAGC GCCGCCGTGT GGAGCGAGAG GAGCGACTGC TCAGGATAGA GAGAGAGGAG 
AGAAACAAGC ACAGCCGCGA CTATGTGGAT AAAATGGAAG AGGCCAGACT TGCGCGAGAG 
GAGAGGTATA AGAACGTGGA GCGCTTGGCC GCAGAGGAGT ATGAGAAGGA GCGTGTCCGA 
GGGTCAAGGA CTCGCCCTGA CCTCAACCTA AGCTTTGAGC CCTCCGAGGC CTGGCCTTCA 
TCGCCGCGCA GCAGCACCCC ATCTTCCTTC GCCTCGCAGG AGGACGCAGA GGCAGAGGTG 
GAGGCGGTGA CGCCAGTCGC GCTCTCTGAG ACGGGGAAAG CCACAGATTC AAGCGACGGC 
GGCGGAGTGG AAGCGGAGGA CAGAGAGGAA GAGCCCGCTG CTGAAGAGGA GGACGAACAC 
AAAGAAGAGG AGGGAGAACA GGAGGAAGCG GCAGCAGAGA AGGAAGAGGA GGAAGCTGAG 
GAGCAGGAGC CGCAGAAGCA GAGAGAAGAG GAGGTGGAGG AGGACAGTGG CATCCTGAGT 
GATAAGGAGC GACAGAATGA GGAAGTAAAC GAGAAAGACA ACTGCTCAGC GTCCAGCATC 
TCCTCTGCCA GCAGCACTTT AGAGCGAGAG GAGAGGGGAA CCGGGGAGAA TGGCCCCCCA 
GGCCTAAAGG AAGCAGAAGT CAGCGACAGT TGCAACAAAC TCCTCAACAG CAAACTCTTC 
ATGCTGGACA TGCTCTACTC CCAAAACAAG AAACCATGCG AGGAGGAGGA GGAGGAAGAG 
GAGGAGGTGG AGAAGGAGAA GGAGAAAGGA GAAGGTGAGG ACAGAGAGAC AGACCAGGAT 
GCCAGCGGAG AACGCGGCGA CGGGGAAAAC AAGAAGGACG ACAAGTCAGA CCATCGCGGG 
AGCATCCGTC AGATTGCCGA GCGGTTCGGA GACCTGATCA AGGACGCCGG CTTGCCCCAC 
CCCCACCCGG ACGACCCGGT CAATGAGCTC CCTCCGCCCC CCGCGCCCAA GAAAGAGTCG 
GACACCATCT GGGACCAGCT CATGTCCAGC CCCCGAGAGC TGCGCATCGG AGACGCCAAC 
TTCACCGACC TGACGGATGA GGACGACAAG GACATTCTGG ACGCTGTTCT GATGGGAGGA 
TGCAGTGACC TCCCACCCCC GCCGCCCCCT CTACCCTTCA TCCCACGCTT TCCACCACCT 
CCACCAGTGC TAGGCATCTG CCCCTTGCCC CCTCCTTTGA TGGGCAGCAT GAGGCCTCCA 
CCTCCACCGT CCTTTAGCTC TCCGATGCCA GGGCTGGCCC CCCAGGAGCA GCCTCTGTTC 
AACAAAAAGA AGAAGACCAT CCGACTCTTC TGGAGTGAAG TTCGGCCCAC AGACTGTCAG 
TACAGGGACT ACAAACGAGG GGGAGACTCA TTCTGGTCCA AACTGGACAC AGTCAAGCTG 
GACACAGCCA AACTGGAGAA CCTGTTTGAG ACCAAGTCAA AGGAGATATC AGTTACAAAG 
AAAACAGCAG CTGATGGCAA ACGTCAGGAG ATCATCGTTT TGGACTCCAA GAGAAGCAAC 
GCCATCAACA TCGGGCTGAC GGTCCTGCCG CCGCCTCGAA CCATCAAGAC AGCCATCCTC 
AACTTTGACG AATACGCTCT CAACAAGGAA GGCATAGAGA AACTCCTGAC AATGATCCCA 
ACGGAGGAGG AGAAGCAGAA GATCCAAGAG GCCCAGTTGG CCAATCCGGA TGTGCCGTTG 
GGTTCTGCAG AGCAGTTTCT CCTCACTCTG TCCTCCATCA GTGAGCTCTC TGCCAGACTC 
CACCTCTGGG CCTTTAAGAT GGACCAAGAA GCTACGGAAA AGGAAGTGGT GGAGCCACTG 
CAAGATCTGA AGGAGGGGAT GGAGCAACTG GAGAAAAACA AAACTCTGCG CTACATCCTC 
TCCACCCTGC TCTCCATTGG AAACTTCCTC AACGGCACCA ATGCCAAAGG CTTTGAGCTG 
ACCTACCTGG AGAAGGTTCC AGAAGTGAAG GACACGGTTC ACAAGCAGTC GCTGCTCCAC 
CATGTCTGCA CGGTGGTGGT GGAAAACTTC CCCCAGAGCA CCGACCTGTA CTCAGAGATC 
GGAGCGATCA CCCGCTCAGC AAAGGTGGAT TTCGACCAGC TGCAGGAAAA CCTGTGCCAG 
ATGGAGCGGC GCTGCAAAGC CTCATGGGAC CATCTGAAAG TCATTGCCAA GCATGAGATG 
AAGCCTCAGC TAAAACAGAA GATGTCCGAC TTCCTTAAAG ACTGCGCCGA GAGGATCATT 
ATCCTGAAGA CGGTTCACCG AAGGATCATC AACAGGTTCC ACTCCTTCCT GCTGTACCTC 
GGCCATCCTC CCTACAGCGT CAGAGAGATC AGCGTCAACA GGTTCAGCAA AATCCTGAGC 
GAGTTTGCGC TGGAGTACCG AACCACCCGC GAGCGCGTGC TGCAGCAGAA GCAGAAGCGA 
GCCGACCACC GCGAACGCAA CAAGACCCGA GGAAAGATGA TCATGGACGT AAACGCTCCT 
TCTGATGATG AAGAAGAGCG TGAGCATTCT GGTCCCGGCG GCTCAAGCAG CAGCAGCGGC 
GCCCCCAGTG GCACCCAGGA GGTGGAGCAG CCTCAGGGGC TGGGCCACGC CGAGGACGCC 
GCAGAGCACG AGCACATGAC AGCAGTGCTG AGGAGCAGCC TGAGCGGCAG CGCCGATAGA 
GAGGGCGGAG TGCCGGGTCT GCGCACCAGA ACCAGGTCCC GGCCTGGACG AGGAGGGCGC 
AGCATGCAGG CGTGGACTCC ACCCGCAGAG GACGCTCAGA CATGTGGAGA CGATGCTGCA 
GACGAGATCA TGGAGCGGAT CGTCCGGTCT GCCACACAGG GTCCAGGAAC CAGAACACAG 
CCCAGAGAGC GCAGGAGGTC CAGGGCCAAC CGGAAATCAT TGAGACGGAC TCTGAAGAAC 
GGCCTCAGCC CAGAGGAAGC GCTGGCTTTA GGCCTGACCG ATTCTCCAGA GACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101171180 ( XM_011481362.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011479664.1 Oryzias latipes FH1/FH2 domain-containing protein 3-like (LOC101171180), mRNA.

Price & Availability↑

CloneID	OJa33837
Clone ID Related Accession (Same CDS sequence)	XM_011481362.2
Accession Version	XM_011481362.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	FH1/FH2 domain-containing protein 3-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019869.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011481362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTCGCTCG ATGACTGCAC ACTTCAGCTC TCCCACAATG GGACCTATCT GGATCTGGAG 
TCCTCCCTGG CCGAGCAAAG GGACGAGCTG GAGGGCTTTC AGCCCGACGA CACTGGGTCC 
AGGGGGAGGA AGCACAGTGT CATTCTACGG ACTCAGCTGA CGGTGCGCGT GCACGCCTGC 
ATCGAAAAAC TCTACAACTG CAGTGGGCGG GACTTAAGGA GGGCGCTGTT CTCCTTGAAG 
CAGATTTTTC AGGATGACAA AGATCTGGTC CATGAGTTTG TGATGGCTGA AGGTCTGACG 
TGTCTCATCA AGGTGGGAGC AGAAGCCGAC CAGAACTATC AGAACTACAT TCTGAGAGCT 
CTGGGGCAGA TCATGCTGTA CGTGGACGGC ATGAACGGAG TCATCAGCCA CCCCGAGACC 
ATCCAGTGGC TCTACACGCT GGTCGGCTCA AAGTTTCGGT TGGTGGTGAA AACGGCTCTG 
AAGCTGCTGC TGGTGTTTGT GGAGTACTCA GAGTCCAACG CGGCGCTGCT CATTCAGGCC 
ATCGCCTCCG TGGACACGAA GGGAGGCTGC AAACCGTGGT CCAATGCCAT GGAGATCCTA 
CATGAGAAGG ATGGGGTGGA CACGGAGTTG CTGGTCTACG CCATGACCCT CATTAATAAG 
ACTTTGGCGG CACTACCGGA TCAGGACTCC TTCTATGACA CGGTGGACAG CTTGGAGGAG 
CAAGGCATGG AGGCGGTCAC CCAGAGGCAC CTGGGGAGGA GAGGCACCGA CCTGGACCTG 
GTTGAGCAGC TCAACATTTA TGAGATGACC CTACGGCACG AAGATGGGGA CGACTGCCAG 
CCCCCATCCA CAGGTCGCCG GGACCGCCAG CGGGCCAGCC TCGGGGGAGG GGACAAACAT 
CAAGGTCTGG AGAGGAGACG GAGCCGGCGG GCATCTCTTG GTCGATCGGG TCAGGCTTCT 
CCGTGCAGCC CAGCGTCACC GAGAGCCGGC TTTCATCCCT TCAGCGGACA CACGGTTGAA 
GACCTGAGTG AAAGAGAGGA GGAGGAGGAG GAGGAGGAGA CTCCTGTGAC TGAGTCAGAT 
TCGGATGAGC AGGAGGGGGC TCATCAGATC ACCTCCAGCC CCCCCAGACC CCCTCCACCC 
ACCGCTCACA GAACGAGCCA TCGCTCCATC ACCTCCAGGA AGGAGAAAGA GCAGAAGAGC 
AGGACCCCTT CGCCCCCAGC AGCCCCACCC TCACCATCTA AACCCCCCTG CAGCCCCCCA 
GCCCACCGCT CCCTGCTATC TACCTTACTG GCACGGAAGC GGTCCATCGT CACCGTTCCA 
GCCACCACGG AGGTCAGTCC TCCTCTGCGC GGCAGCTCCC GCCCCTCCCC GGACCGCCTG 
CCCTACCTGC CCCACTCCCC CTTCCACCTC TTCTCATACG ATCTTGAGGA GGAGGCGTCC 
GCTTCGGCCC AGACCAAACG CAGCACGGAG TCGGCATCTC CAAGGAACGG AGATCCTGCG 
TCTCACACCT CGTTCAGCTC GCCAAGCACG CCCACCAGCT CCAGACCGGC TCTGGGAGGA 
CTGCTGTCAT CCTCCTACAG GCAGCACCAG GAGTCTCTGG CTGCTGAGCG AGAGCGCCGC 
CGTGTGGAGC GAGAGGAGCG ACTGCTCAGG ATAGAGAGAG AGGAGAGAAA CAAGCACAGC 
CGCGACTATG TGGATAAAAT GGAAGAGGCC AGACTTGCGC GAGAGGAGAG GTATAAGAAC 
GTGGAGCGCT TGGCCGCAGA GGAGTATGAG AAGGAGCGTG TCCGAGGGTC AAGGACTCGC 
CCTGACCTCA ACCTAAGCTT TGAGCCCTCC GAGGCCTGGC CTTCATCGCC GCGCAGCAGC 
ACCCCATCTT CCTTCGCCTC GCAGGAGGAC GCAGAGGCAG AGGTGGAGGC GGTGACGCCA 
GTCGCGCTCT CTGAGACGGG GAAAGCCACA GATTCAAGCG ACGGCGGCGG AGTGGAAGCG 
GAGGACAGAG AGGAAGAGCC CGCTGCTGAA GAGGAGGACG AACACAAAGA AGAGGAGGGA 
GAACAGGAGG AAGCGGCAGC AGAGAAGGAA GAGGAGGAAG CTGAGGAGCA GGAGCCGCAG 
AAGCAGAGAG AAGAGGAGGT GGAGGAGGAC AGTGGCATCC TGAGTGATAA GGAGCGACAG 
AATGAGGAAG TAAACGAGAA AGACAACTGC TCAGCGTCCA GCATCTCCTC TGCCAGCAGC 
ACTTTAGAGC GAGAGGAGAG GGGAACCGGG GAGAATGGCC CCCCAGGCCT AAAGGAAGCA 
GAAGTCAGCG ACAGTTGCAA CAAACTCCTC AACAGCAAAC TCTTCATGCT GGACATGCTC 
TACTCCCAAA ACAAGAAACC ATGCGAGGAG GAGGAGGAGG AAGAGGAGGA GGTGGAGAAG 
GAGAAGGAGA AAGGAGAAGG TGAGGACAGA GAGACAGANC AGGATGCCAG CGGAGAACGC 
GGCGACGGGG AAAACAAGAA GGACGACAAG TCAGACCATC GCGGGAGCAT CCGTCAGATT 
GCCGAGCGGT TCGGAGACCT GATCAAGGAC GCCGGCTTGC CCCACCCCCA CCCGGACGAC 
CCGGTCAATG AGCTCCCTCC GCCCCCCGCG CCCAAGAAAG AGTCGGACAC CATCTGGGAC 
CAGCTCATGT CCAGCCCCCG AGAGCTGCGC ATCGGAGACG CCAACTTCAC CGACCTGACG 
GATGAGGACG ACAAGGACAT TCTGGACGCT GTTCTGATGG GAGGATGCAG TGACCTCCCA 
CCCCCGCCGC CCCCTCTACC CTTCATCCCA CGCTTTCCAC CACCTCCACC AGTGCTAGGC 
ATCTGCCCCT TGCCCCCTCC TTTGATGGGC AGCATGAGGC CTCCACCTCC ACCGTCCTTT 
AGCTCTCCGA TGCCAGGGCT GGCCCCCCAG GAGCAGCCTC TGTTCAACAA AAAGAAGAAG 
ACCATCCGAC TCTTCTGGAG TGAAGTTCGG CCCACAGACT GTCAGTACAG GGACTACAAA 
CGAGGGGGAG ACTCATTCTG GTCCAAACTG GACACAGTCA AGCTGGACAC AGCCAAACTG 
GAGAACCTGT TTGAGACCAA GTCAAAGGAG ATATCAGTTA CAAAGAAAAC AGCAGCTGAT 
GGCAAACGTC AGGAGATCAT CGTTTTGGAC TCCAAGAGAA GCAACGCCAT CAACATCGGG 
CTGACGGTCC TGCCGCCGCC TCGAACCATC AAGACAGCCA TCCTCAACTT TGACGAATAC 
GCTCTCAACA AGGAAGGCAT AGAGAAACTC CTGACAATGA TCCCAACGGA GGAGGAGAAG 
CAGAAGATCC AAGAGGCCCA GTTGGCCAAT CCGGATGTGC CGTTGGGTTC TGCAGAGCAG 
TTTCTCCTCA CTCTGTCCTC CATCAGTGAG CTCTCTGCCA GACTCCACCT CTGGGCCTTT 
AAGATGGACC AAGAAGCTAC GGAAAAGGAA GTGGTGGAGC CACTGCAAGA TCTGAAGGAG 
GGGATGGAGC AACTGGAGAA AAACAAAACT CTGCGCTACA TCCTCTCCAC CCTGCTCTCC 
ATTGGAAACT TCCTCAACGG CACCAATGCC AAAGGCTTTG AGCTGACCTA CCTGGAGAAG 
GTTCCAGAAG TGAAGGACAC GGTTCACAAG CAGTCGCTGC TCCACCATGT CTGCACGGTG 
GTGGTGGAAA ACTTCCCCCA GAGCACCGAC CTGTACTCAG AGATCGGAGC GATCACCCGC 
TCAGCAAAGG TGGATTTCGA CCAGCTGCAG GAAAACCTGT GCCAGATGGA GCGGCGCTGC 
AAAGCCTCAT GGGACCATCT GAAAGTCATT GCCAAGCATG AGATGAAGCC TCAGCTAAAA 
CAGAAGATGT CCGACTTCCT TAAAGACTGC GCCGAGAGGA TCATTATCCT GAAGACGGTT 
CACCGAAGGA TCATCAACAG GTTCCACTCC TTCCTGCTGT ACCTCGGCCA TCCTCCCTAC 
AGCGTCAGAG AGATCAGCGT CAACAGGTTC AGCAAAATCC TGAGCGAGTT TGCGCTGGAG 
TACCGAACCA CCCGCGAGCG CGTGCTGCAG CAGAAGCAGA AGCGAGCCGA CCACCGCGAA 
CGCAACAAGA CCCGAGGAAA GATGATCATG GACGTAAACG CTCCTTCTGA TGATGAAGAA 
GAGCGTGAGC ATTCTGGTCC CGGCGGCTCA AGCAGCAGCA GCGGCGCCCC CAGTGGCACC 
CAGGAGGTGG AGCAGCCTCA GGGGCTGGGC CACGCCGAGG ACGCCGCAGA GCACGAGCAC 
ATGACAGCAG TGCTGAGGAG CAGCCTGAGC GGCAGCGCCG ATAGAGAGGG CGGAGTGCCG 
GGTCTGCGCA CCAGAACCAG GTCCCGGCCT GGACGAGGAG GGCGCAGCAT GCAGGCGTGG 
ACTCCACCCG CAGAGGACGC TCAGACATGT GGAGACGATG CTGCAGACGA GATCATGGAG 
CGGATCGTCC GGTCTGCCAC ACAGGGTCCA GGAACCAGAA CACAGCCCAG AGAGCGCAGG 
AGGTCCAGGG CCAACCGGAA ATCATTGAGA CGGACTCTGA AGAACGGCCT CAGCCCAGAG 
GAAGCGCTGG CTTTAGGCCT GACCGATTCT CCAGAGACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011479664.1
CDS	1..4602
Translation	MSLDDCTLQLSHNGTYLDLESSLAEQRDELEGFQPDDTGSRGRKHSVILRTQLTVRVHACIEKLYNCSGRDLRRALFSLKQIFQDDKDLVHEFVMAEGLTCLIKVGAEADQNYQNYILRALGQIMLYVDGMNGVISHPETIQWLYTLVGSKFRLVVKTALKLLLVFVEYSESNAALLIQAIASVDTKGGCKPWSNAMEILHEKDGVDTELLVYAMTLINKTLAALPDQDSFYDTVDSLEEQGMEAVTQRHLGRRGTDLDLVEQLNIYEMTLRHEDGDDCQPPSTGRRDRQRASLGGGDKHQGLERRRSRRASLGRSGQASPCSPASPRAGFHPFSGHTVEDLSEREEEEEEEETPVTESDSDEQEGAHQITSSPPRPPPPTAHRTSHRSITSRKEKEQKSRTPSPPAAPPSPSKPPCSPPAHRSLLSTLLARKRSIVTVPATTEVSPPLRGSSRPSPDRLPYLPHSPFHLFSYDLEEEASASAQTKRSTESASPRNGDPASHTSFSSPSTPTSSRPALGGLLSSSYRQHQESLAAERERRRVEREERLLRIEREERNKHSRDYVDKMEEARLAREERYKNVERLAAEEYEKERVRGSRTRPDLNLSFEPSEAWPSSPRSSTPSSFASQEDAEAEVEAVTPVALSETGKATDSSDGGGVEAEDREEEPAAEEEDEHKEEEGEQEEAAAEKEEEEAEEQEPQKQREEEVEEDSGILSDKERQNEEVNEKDNCSASSISSASSTLEREERGTGENGPPGLKEAEVSDSCNKLLNSKLFMLDMLYSQNKKPCEEEEEEEEEVEKEKEKGEGEDRETnullQDASGERGDGENKKDDKSDHRGSIRQIAERFGDLIKDAGLPHPHPDDPVNELPPPPAPKKESDTIWDQLMSSPRELRIGDANFTDLTDEDDKDILDAVLMGGCSDLPPPPPPLPFIPRFPPPPPVLGICPLPPPLMGSMRPPPPPSFSSPMPGLAPQEQPLFNKKKKTIRLFWSEVRPTDCQYRDYKRGGDSFWSKLDTVKLDTAKLENLFETKSKEISVTKKTAADGKRQEIIVLDSKRSNAINIGLTVLPPPRTIKTAILNFDEYALNKEGIEKLLTMIPTEEEKQKIQEAQLANPDVPLGSAEQFLLTLSSISELSARLHLWAFKMDQEATEKEVVEPLQDLKEGMEQLEKNKTLRYILSTLLSIGNFLNGTNAKGFELTYLEKVPEVKDTVHKQSLLHHVCTVVVENFPQSTDLYSEIGAITRSAKVDFDQLQENLCQMERRCKASWDHLKVIAKHEMKPQLKQKMSDFLKDCAERIIILKTVHRRIINRFHSFLLYLGHPPYSVREISVNRFSKILSEFALEYRTTRERVLQQKQKRADHRERNKTRGKMIMDVNAPSDDEEEREHSGPGGSSSSSGAPSGTQEVEQPQGLGHAEDAAEHEHMTAVLRSSLSGSADREGGVPGLRTRTRSRPGRGGRSMQAWTPPAEDAQTCGDDAADEIMERIVRSATQGPGTRTQPRERRRSRANRKSLRRTLKNGLSPEEALALGLTDSPET

Target ORF information:

RefSeq Version	XM_011481362.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes FH1/FH2 domain-containing protein 3-like (LOC101171180), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011481362.2

ORF Insert Sequence:

ATGTCGCTCG ATGACTGCAC ACTTCAGCTC TCCCACAATG GGACCTATCT GGATCTGGAG 
TCCTCCCTGG CCGAGCAAAG GGACGAGCTG GAGGGCTTTC AGCCCGACGA CACTGGGTCC 
AGGGGGAGGA AGCACAGTGT CATTCTACGG ACTCAGCTGA CGGTGCGCGT GCACGCCTGC 
ATCGAAAAAC TCTACAACTG CAGTGGGCGG GACTTAAGGA GGGCGCTGTT CTCCTTGAAG 
CAGATTTTTC AGGATGACAA AGATCTGGTC CATGAGTTTG TGATGGCTGA AGGTCTGACG 
TGTCTCATCA AGGTGGGAGC AGAAGCCGAC CAGAACTATC AGAACTACAT TCTGAGAGCT 
CTGGGGCAGA TCATGCTGTA CGTGGACGGC ATGAACGGAG TCATCAGCCA CCCCGAGACC 
ATCCAGTGGC TCTACACGCT GGTCGGCTCA AAGTTTCGGT TGGTGGTGAA AACGGCTCTG 
AAGCTGCTGC TGGTGTTTGT GGAGTACTCA GAGTCCAACG CGGCGCTGCT CATTCAGGCC 
ATCGCCTCCG TGGACACGAA GGGAGGCTGC AAACCGTGGT CCAATGCCAT GGAGATCCTA 
CATGAGAAGG ATGGGGTGGA CACGGAGTTG CTGGTCTACG CCATGACCCT CATTAATAAG 
ACTTTGGCGG CACTACCGGA TCAGGACTCC TTCTATGACA CGGTGGACAG CTTGGAGGAG 
CAAGGCATGG AGGCGGTCAC CCAGAGGCAC CTGGGGAGGA GAGGCACCGA CCTGGACCTG 
GTTGAGCAGC TCAACATTTA TGAGATGACC CTACGGCACG AAGATGGGGA CGACTGCCAG 
CCCCCATCCA CAGGTCGCCG GGACCGCCAG CGGGCCAGCC TCGGGGGAGG GGACAAACAT 
CAAGGTCTGG AGAGGAGACG GAGCCGGCGG GCATCTCTTG GTCGATCGGG TCAGGCTTCT 
CCGTGCAGCC CAGCGTCACC GAGAGCCGGC TTTCATCCCT TCAGCGGACA CACGGTTGAA 
GACCTGAGTG AAAGAGAGGA GGAGGAGGAG GAGGAGGAGA CTCCTGTGAC TGAGTCAGAT 
TCGGATGAGC AGGAGGGGGC TCATCAGATC ACCTCCAGCC CCCCCAGACC CCCTCCACCC 
ACCGCTCACA GAACGAGCCA TCGCTCCATC ACCTCCAGGA AGGAGAAAGA GCAGAAGAGC 
AGGACCCCTT CGCCCCCAGC AGCCCCACCC TCACCATCTA AACCCCCCTG CAGCCCCCCA 
GCCCACCGCT CCCTGCTATC TACCTTACTG GCACGGAAGC GGTCCATCGT CACCGTTCCA 
GCCACCACGG AGGTCAGTCC TCCTCTGCGC GGCAGCTCCC GCCCCTCCCC GGACCGCCTG 
CCCTACCTGC CCCACTCCCC CTTCCACCTC TTCTCATACG ATCTTGAGGA GGAGGCGTCC 
GCTTCGGCCC AGACCAAACG CAGCACGGAG TCGGCATCTC CAAGGAACGG AGATCCTGCG 
TCTCACACCT CGTTCAGCTC GCCAAGCACG CCCACCAGCT CCAGACCGGC TCTGGGAGGA 
CTGCTGTCAT CCTCCTACAG GCAGCACCAG GAGTCTCTGG CTGCTGAGCG AGAGCGCCGC 
CGTGTGGAGC GAGAGGAGCG ACTGCTCAGG ATAGAGAGAG AGGAGAGAAA CAAGCACAGC 
CGCGACTATG TGGATAAAAT GGAAGAGGCC AGACTTGCGC GAGAGGAGAG GTATAAGAAC 
GTGGAGCGCT TGGCCGCAGA GGAGTATGAG AAGGAGCGTG TCCGAGGGTC AAGGACTCGC 
CCTGACCTCA ACCTAAGCTT TGAGCCCTCC GAGGCCTGGC CTTCATCGCC GCGCAGCAGC 
ACCCCATCTT CCTTCGCCTC GCAGGAGGAC GCAGAGGCAG AGGTGGAGGC GGTGACGCCA 
GTCGCGCTCT CTGAGACGGG GAAAGCCACA GATTCAAGCG ACGGCGGCGG AGTGGAAGCG 
GAGGACAGAG AGGAAGAGCC CGCTGCTGAA GAGGAGGACG AACACAAAGA AGAGGAGGGA 
GAACAGGAGG AAGCGGCAGC AGAGAAGGAA GAGGAGGAAG CTGAGGAGCA GGAGCCGCAG 
AAGCAGAGAG AAGAGGAGGT GGAGGAGGAC AGTGGCATCC TGAGTGATAA GGAGCGACAG 
AATGAGGAAG TAAACGAGAA AGACAACTGC TCAGCGTCCA GCATCTCCTC TGCCAGCAGC 
ACTTTAGAGC GAGAGGAGAG GGGAACCGGG GAGAATGGCC CCCCAGGCCT AAAGGAAGCA 
GAAGTCAGCG ACAGTTGCAA CAAACTCCTC AACAGCAAAC TCTTCATGCT GGACATGCTC 
TACTCCCAAA ACAAGAAACC ATGCGAGGAG GAGGAGGAGG AAGAGGAGGA GGTGGAGAAG 
GAGAAGGAGA AAGGAGAAGG TGAGGACAGA GAGACAGANC AGGATGCCAG CGGAGAACGC 
GGCGACGGGG AAAACAAGAA GGACGACAAG TCAGACCATC GCGGGAGCAT CCGTCAGATT 
GCCGAGCGGT TCGGAGACCT GATCAAGGAC GCCGGCTTGC CCCACCCCCA CCCGGACGAC 
CCGGTCAATG AGCTCCCTCC GCCCCCCGCG CCCAAGAAAG AGTCGGACAC CATCTGGGAC 
CAGCTCATGT CCAGCCCCCG AGAGCTGCGC ATCGGAGACG CCAACTTCAC CGACCTGACG 
GATGAGGACG ACAAGGACAT TCTGGACGCT GTTCTGATGG GAGGATGCAG TGACCTCCCA 
CCCCCGCCGC CCCCTCTACC CTTCATCCCA CGCTTTCCAC CACCTCCACC AGTGCTAGGC 
ATCTGCCCCT TGCCCCCTCC TTTGATGGGC AGCATGAGGC CTCCACCTCC ACCGTCCTTT 
AGCTCTCCGA TGCCAGGGCT GGCCCCCCAG GAGCAGCCTC TGTTCAACAA AAAGAAGAAG 
ACCATCCGAC TCTTCTGGAG TGAAGTTCGG CCCACAGACT GTCAGTACAG GGACTACAAA 
CGAGGGGGAG ACTCATTCTG GTCCAAACTG GACACAGTCA AGCTGGACAC AGCCAAACTG 
GAGAACCTGT TTGAGACCAA GTCAAAGGAG ATATCAGTTA CAAAGAAAAC AGCAGCTGAT 
GGCAAACGTC AGGAGATCAT CGTTTTGGAC TCCAAGAGAA GCAACGCCAT CAACATCGGG 
CTGACGGTCC TGCCGCCGCC TCGAACCATC AAGACAGCCA TCCTCAACTT TGACGAATAC 
GCTCTCAACA AGGAAGGCAT AGAGAAACTC CTGACAATGA TCCCAACGGA GGAGGAGAAG 
CAGAAGATCC AAGAGGCCCA GTTGGCCAAT CCGGATGTGC CGTTGGGTTC TGCAGAGCAG 
TTTCTCCTCA CTCTGTCCTC CATCAGTGAG CTCTCTGCCA GACTCCACCT CTGGGCCTTT 
AAGATGGACC AAGAAGCTAC GGAAAAGGAA GTGGTGGAGC CACTGCAAGA TCTGAAGGAG 
GGGATGGAGC AACTGGAGAA AAACAAAACT CTGCGCTACA TCCTCTCCAC CCTGCTCTCC 
ATTGGAAACT TCCTCAACGG CACCAATGCC AAAGGCTTTG AGCTGACCTA CCTGGAGAAG 
GTTCCAGAAG TGAAGGACAC GGTTCACAAG CAGTCGCTGC TCCACCATGT CTGCACGGTG 
GTGGTGGAAA ACTTCCCCCA GAGCACCGAC CTGTACTCAG AGATCGGAGC GATCACCCGC 
TCAGCAAAGG TGGATTTCGA CCAGCTGCAG GAAAACCTGT GCCAGATGGA GCGGCGCTGC 
AAAGCCTCAT GGGACCATCT GAAAGTCATT GCCAAGCATG AGATGAAGCC TCAGCTAAAA 
CAGAAGATGT CCGACTTCCT TAAAGACTGC GCCGAGAGGA TCATTATCCT GAAGACGGTT 
CACCGAAGGA TCATCAACAG GTTCCACTCC TTCCTGCTGT ACCTCGGCCA TCCTCCCTAC 
AGCGTCAGAG AGATCAGCGT CAACAGGTTC AGCAAAATCC TGAGCGAGTT TGCGCTGGAG 
TACCGAACCA CCCGCGAGCG CGTGCTGCAG CAGAAGCAGA AGCGAGCCGA CCACCGCGAA 
CGCAACAAGA CCCGAGGAAA GATGATCATG GACGTAAACG CTCCTTCTGA TGATGAAGAA 
GAGCGTGAGC ATTCTGGTCC CGGCGGCTCA AGCAGCAGCA GCGGCGCCCC CAGTGGCACC 
CAGGAGGTGG AGCAGCCTCA GGGGCTGGGC CACGCCGAGG ACGCCGCAGA GCACGAGCAC 
ATGACAGCAG TGCTGAGGAG CAGCCTGAGC GGCAGCGCCG ATAGAGAGGG CGGAGTGCCG 
GGTCTGCGCA CCAGAACCAG GTCCCGGCCT GGACGAGGAG GGCGCAGCAT GCAGGCGTGG 
ACTCCACCCG CAGAGGACGC TCAGACATGT GGAGACGATG CTGCAGACGA GATCATGGAG 
CGGATCGTCC GGTCTGCCAC ACAGGGTCCA GGAACCAGAA CACAGCCCAG AGAGCGCAGG 
AGGTCCAGGG CCAACCGGAA ATCATTGAGA CGGACTCTGA AGAACGGCCT CAGCCCAGAG 
GAAGCGCTGG CTTTAGGCCT GACCGATTCT CCAGAGACGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC101171180 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

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gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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