LOC101160262 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101160262
Entrez Gene ID	101160262
Full Name	sphingosine kinase 2
Synonyms	sphk2
Gene Type	protein-coding
Organism	Oryzias latipes(Japanese medaka)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011485353.3	XP_011483655.1	sphingosine kinase 2
XM_011485353.2	XP_011483655.1	sphingosine kinase 2

Pathways from BioSystems
Gene Ontology

The following LOC101160262 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101160262 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OJa25489	XM_011485353.3 Latest version!	Oryzias latipes sphingosine kinase 2 (LOC101160262), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OJa25489	XM_011485353.2	Oryzias latipes sphingosine kinase 2 (LOC101160262), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101160262 ( XM_011485353.3 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011483655.1 Oryzias latipes sphingosine kinase 2 (LOC101160262), mRNA.

Price & Availability↑

CloneID	OJa25489
Clone ID Related Accession (Same CDS sequence)	XM_011485353.3 , XM_011485353.2
Accession Version	XM_011485353.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1518537600000
Organism	Oryzias latipes(Japanese medaka)
Product	sphingosine kinase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019874.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 15, 2018 this sequence version replaced XM_011485353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Oryzias latipes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATCCC CTGAACCGTC CTCGTCGCCT CCAGCAGAAG CCCTGCTTCA TGGCCAGTTT 
GCTAGCTGGG GGTCAGGCAC CAACAGCAAC AGTAACAGCC GTCCCAGCAG CCCCGGCGGC 
CTCGGAGGAC TTTCCCCTGC TGCATCCCCA ACATCTCCTC CAGTGTCCAA CTATGCCTTG 
ACTCTCACGC ACAGCCACAT TCACATTCAG AGGCTGTCCC CAAAGTCAGG GAAGGAAGCC 
CGACTGCTGC TGCCTCTGTC TGAGCTGGTG GGGTGCAGCT GCCCCCGCTC CCCTGCACCC 
CCACTGTTTG TGTTGTACTG GTACCCCCCA GGGAAACGAA GGAAAGGGGT GTCCCGTCGC 
AGGCAGGTGA GGGCCTATCT AGCTGAAAGC AGGCCTGAAG CTGAAAGGTG GTCTGCTGCT 
GTACAGTGTC TCCTCAGAGG GGTGACTGTT ACTGCTGACA CCGAATTTTC GAGAAGTCTT 
CTACCTCGTC CCAGGCGGCT GCTGTTATTG GTGAATCCAT TCAGTGGAAG GGGCCAAGCA 
ATGCAATGGT GTCAGACCCA CATCCTGCCA ATGATTAGAG AGGCCAATAT CAGCTACAAC 
CTCATACAGA CTGAACGGCA GAATCATGCA AGAGAGCTCA TCAGAGAAGT GTCCCTCTCT 
GACTGGGATG GCATCGTCAT TGTCTCTGGA GATGGCTTGT TGCATGAGGT AATTAATGGT 
TTAATGGAGC GTCCAGACTG GGAACAAGCA ATAAAAACAC CTGTCGGCAT TCTGCCCTGC 
GGTTCCGGGA ATGCGCTGGC TGGTTCCATC AATCACAACG CAGGGTATGA CATGTGCCTT 
CGAGAGCCCC TCCTATTGAA CTGCTGCTTT CTGCTATGTC GAGGCGGGGT GCGACCGATG 
GACGTGGTCT CCGTGACAAC GAGCCCTGCT CCGTCCAACA ACAGTCATCC TGCAGCACCC 
AGGAGAGTCT TTTCCTTTCT TTCTGTTGCC TGGGGCTTTG TGTCTGATGT AGACATTGAA 
AGTGAGAGAT ATCGTGGCTT GGGCTCAGCC CGCTTCACCC TGGGCACTTT AGTTCGCATA 
GCTTCGCTCC GATCCTACAA AGGCCGCCTG TCCTTTCTGC CTCCCTCTGT CGTCCCTTCA 
TCCTCAGATG GCACGACCCC CCCGCCGAGG AGACCCCTGT CCCGCAGCAT CACTGAAGGC 
CTGGAGGGGT TTTGCCGATT GCCCATACAC CGCACCTGCT CTGACATGGG CATCAGCGAG 
CAAAGAAGTC TGCGGAGGGG TGAAGGAGAA AGGGAAAAGG AGGAGAGGCA GAGGGAAAGG 
GAGAGGAGAA GGGAGAGGGC CAGAGGGGGA GGAACTGGTG TGGTGAGGGC GAGCAGCCTT 
GCGGAGGACA GGGAAAAGGA GGGGGAGCTG GAGGCAGAGG ATGACAAGTC AAGGACTTGT 
TCAGAAAGGT CCGGGACAAG TTCTGAATCG AATGAAATGA ATAAAGAAAG GTTGGAGAAA 
ATTGAAGACG AGGGAACATT TGGACAAGAG TCTGTTGAGA TGGAGGAGGA TAGAGGGCAG 
GATGGAGGAG GGAGTGGCGA CTCTGTTGAA GAAGAGGAAG CGTTAGCCGC AAGAGAGCTA 
GATGAGGGCT ACGGGGTGGA CATGGGGCAA GAGGCGGATG AAGAAACAGA GGAGTCCATC 
ACTTACCCAG ACGACCCTCA GCGTGCCAGA CAGGGCGTTA GGAAGAATTC AGCCCCGTCC 
AGTGAGATCT GTAACGACCT GTCGAGTCAG CCTCTGCATA AGGTGGCTGA CGCAGATTCA 
AGCACAGCAG ATGGGGTGGA GGACATAGAT CTGAATGGAA CCTACTTCCA GAAAGATCCT 
TACACAGATG TGGCTCGGGA GCGATCTCTG ACCATCTCCT CTCCTTTTCG TCATTCTCCC 
TTTTCTTTCA AGCATAAAGC CCTGGACCAA AACCAAAACG CTTCCCGTCC AAGACCTCTG 
TCACTCCTGC AGCACTCCCA CACTAACTCT CTCCCCCCTA AAATGCCTTC CCTTTCACTG 
TCTCCTACTC CCCCGCCCTC CCCCTCTTGC GCCTCTCCAC ACTCATCCTA TTACCTTAAC 
CCTCGTCCAA ACACGCCAAA TTCCTCCTCG CCCTCTCCGT CTGTCCGCAC ACCGTCCTCA 
TCTTTCAACT TTGACATTTC CGAAGCAGCC AGACCCTTCA AGAACCGCCC GTTGGTCACG 
TTGCCTCCAG ATCTACCGAG AGACAACTTG TTACCCCCTC TTGACCAACC CCTCCCCACT 
AGAGACTGGG TGACCATCGA AGGGGACTTT GTGTTGGTCT TGGCCCTTTA TCAAACCCAC 
CTGGGAGCTG ACCTTCACGC CGCACCTCAA GCCAAGTTTG ACGACGGGCT CATTCACCTG 
ACATTTGTGC GGGCAGGAAT CTCCAGAGCC ACGCTACTAA GATTGTTCTT TGCCATGGAG 
AGAGGAACCC ACCACTCCGT CAGCTCGCCG TACGTGAGCC ACGTTACCTG CAAGGCCTTC 
AGGCTGCAAC CTCTATCTGC AAAAGGAACG CTCACCGTCG ATGGAGAACT TGTTCCTTAT 
GGGCCACTCC AGGCACAGGT TCATCCTTCC ATGGCTCGTC TCATTGTTGG AGACTACGGA 
GTGAAGATTA CCAGCTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011483655.1
CDS	1281..3941
Translation	MRSPEPSSSPPAEALLHGQFASWGSGTNSNSNSRPSSPGGLGGLSPAASPTSPPVSNYALTLTHSHIHIQRLSPKSGKEARLLLPLSELVGCSCPRSPAPPLFVLYWYPPGKRRKGVSRRRQVRAYLAESRPEAERWSAAVQCLLRGVTVTADTEFSRSLLPRPRRLLLLVNPFSGRGQAMQWCQTHILPMIREANISYNLIQTERQNHARELIREVSLSDWDGIVIVSGDGLLHEVINGLMERPDWEQAIKTPVGILPCGSGNALAGSINHNAGYDMCLREPLLLNCCFLLCRGGVRPMDVVSVTTSPAPSNNSHPAAPRRVFSFLSVAWGFVSDVDIESERYRGLGSARFTLGTLVRIASLRSYKGRLSFLPPSVVPSSSDGTTPPPRRPLSRSITEGLEGFCRLPIHRTCSDMGISEQRSLRRGEGEREKEERQRERERRRERARGGGTGVVRASSLAEDREKEGELEAEDDKSRTCSERSGTSSESNEMNKERLEKIEDEGTFGQESVEMEEDRGQDGGGSGDSVEEEEALAARELDEGYGVDMGQEADEETEESITYPDDPQRARQGVRKNSAPSSEICNDLSSQPLHKVADADSSTADGVEDIDLNGTYFQKDPYTDVARERSLTISSPFRHSPFSFKHKALDQNQNASRPRPLSLLQHSHTNSLPPKMPSLSLSPTPPPSPSCASPHSSYYLNPRPNTPNSSSPSPSVRTPSSSFNFDISEAARPFKNRPLVTLPPDLPRDNLLPPLDQPLPTRDWVTIEGDFVLVLALYQTHLGADLHAAPQAKFDDGLIHLTFVRAGISRATLLRLFFAMERGTHHSVSSPYVSHVTCKAFRLQPLSAKGTLTVDGELVPYGPLQAQVHPSMARLIVGDYGVKITSF

Target ORF information:

RefSeq Version	XM_011485353.3
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes sphingosine kinase 2 (LOC101160262), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011485353.3

ORF Insert Sequence:

ATGCGATCCC CTGAACCGTC CTCGTCGCCT CCAGCAGAAG CCCTGCTTCA TGGCCAGTTT 
GCTAGCTGGG GGTCAGGCAC CAACAGCAAC AGTAACAGCC GTCCCAGCAG CCCCGGCGGC 
CTCGGAGGAC TTTCCCCTGC TGCATCCCCA ACATCTCCTC CAGTGTCCAA CTATGCCTTG 
ACTCTCACGC ACAGCCACAT TCACATTCAG AGGCTGTCCC CAAAGTCAGG GAAGGAAGCC 
CGACTGCTGC TGCCTCTGTC TGAGCTGGTG GGGTGCAGCT GCCCCCGCTC CCCTGCACCC 
CCACTGTTTG TGTTGTACTG GTACCCCCCA GGGAAACGAA GGAAAGGGGT GTCCCGTCGC 
AGGCAGGTGA GGGCCTATCT AGCTGAAAGC AGGCCTGAAG CTGAAAGGTG GTCTGCTGCT 
GTACAGTGTC TCCTCAGAGG GGTGACTGTT ACTGCTGACA CCGAATTTTC GAGAAGTCTT 
CTACCTCGTC CCAGGCGGCT GCTGTTATTG GTGAATCCAT TCAGTGGAAG GGGCCAAGCA 
ATGCAATGGT GTCAGACCCA CATCCTGCCA ATGATTAGAG AGGCCAATAT CAGCTACAAC 
CTCATACAGA CTGAACGGCA GAATCATGCA AGAGAGCTCA TCAGAGAAGT GTCCCTCTCT 
GACTGGGATG GCATCGTCAT TGTCTCTGGA GATGGCTTGT TGCATGAGGT AATTAATGGT 
TTAATGGAGC GTCCAGACTG GGAACAAGCA ATAAAAACAC CTGTCGGCAT TCTGCCCTGC 
GGTTCCGGGA ATGCGCTGGC TGGTTCCATC AATCACAACG CAGGGTATGA CATGTGCCTT 
CGAGAGCCCC TCCTATTGAA CTGCTGCTTT CTGCTATGTC GAGGCGGGGT GCGACCGATG 
GACGTGGTCT CCGTGACAAC GAGCCCTGCT CCGTCCAACA ACAGTCATCC TGCAGCACCC 
AGGAGAGTCT TTTCCTTTCT TTCTGTTGCC TGGGGCTTTG TGTCTGATGT AGACATTGAA 
AGTGAGAGAT ATCGTGGCTT GGGCTCAGCC CGCTTCACCC TGGGCACTTT AGTTCGCATA 
GCTTCGCTCC GATCCTACAA AGGCCGCCTG TCCTTTCTGC CTCCCTCTGT CGTCCCTTCA 
TCCTCAGATG GCACGACCCC CCCGCCGAGG AGACCCCTGT CCCGCAGCAT CACTGAAGGC 
CTGGAGGGGT TTTGCCGATT GCCCATACAC CGCACCTGCT CTGACATGGG CATCAGCGAG 
CAAAGAAGTC TGCGGAGGGG TGAAGGAGAA AGGGAAAAGG AGGAGAGGCA GAGGGAAAGG 
GAGAGGAGAA GGGAGAGGGC CAGAGGGGGA GGAACTGGTG TGGTGAGGGC GAGCAGCCTT 
GCGGAGGACA GGGAAAAGGA GGGGGAGCTG GAGGCAGAGG ATGACAAGTC AAGGACTTGT 
TCAGAAAGGT CCGGGACAAG TTCTGAATCG AATGAAATGA ATAAAGAAAG GTTGGAGAAA 
ATTGAAGACG AGGGAACATT TGGACAAGAG TCTGTTGAGA TGGAGGAGGA TAGAGGGCAG 
GATGGAGGAG GGAGTGGCGA CTCTGTTGAA GAAGAGGAAG CGTTAGCCGC AAGAGAGCTA 
GATGAGGGCT ACGGGGTGGA CATGGGGCAA GAGGCGGATG AAGAAACAGA GGAGTCCATC 
ACTTACCCAG ACGACCCTCA GCGTGCCAGA CAGGGCGTTA GGAAGAATTC AGCCCCGTCC 
AGTGAGATCT GTAACGACCT GTCGAGTCAG CCTCTGCATA AGGTGGCTGA CGCAGATTCA 
AGCACAGCAG ATGGGGTGGA GGACATAGAT CTGAATGGAA CCTACTTCCA GAAAGATCCT 
TACACAGATG TGGCTCGGGA GCGATCTCTG ACCATCTCCT CTCCTTTTCG TCATTCTCCC 
TTTTCTTTCA AGCATAAAGC CCTGGACCAA AACCAAAACG CTTCCCGTCC AAGACCTCTG 
TCACTCCTGC AGCACTCCCA CACTAACTCT CTCCCCCCTA AAATGCCTTC CCTTTCACTG 
TCTCCTACTC CCCCGCCCTC CCCCTCTTGC GCCTCTCCAC ACTCATCCTA TTACCTTAAC 
CCTCGTCCAA ACACGCCAAA TTCCTCCTCG CCCTCTCCGT CTGTCCGCAC ACCGTCCTCA 
TCTTTCAACT TTGACATTTC CGAAGCAGCC AGACCCTTCA AGAACCGCCC GTTGGTCACG 
TTGCCTCCAG ATCTACCGAG AGACAACTTG TTACCCCCTC TTGACCAACC CCTCCCCACT 
AGAGACTGGG TGACCATCGA AGGGGACTTT GTGTTGGTCT TGGCCCTTTA TCAAACCCAC 
CTGGGAGCTG ACCTTCACGC CGCACCTCAA GCCAAGTTTG ACGACGGGCT CATTCACCTG 
ACATTTGTGC GGGCAGGAAT CTCCAGAGCC ACGCTACTAA GATTGTTCTT TGCCATGGAG 
AGAGGAACCC ACCACTCCGT CAGCTCGCCG TACGTGAGCC ACGTTACCTG CAAGGCCTTC 
AGGCTGCAAC CTCTATCTGC AAAAGGAACG CTCACCGTCG ATGGAGAACT TGTTCCTTAT 
GGGCCACTCC AGGCACAGGT TCATCCTTCC ATGGCTCGTC TCATTGTTGG AGACTACGGA 
GTGAAGATTA CCAGCTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101160262 ( XM_011485353.2 ) cDNA ORF clone, Oryzias latipes(Japanese medaka) -> XP_011483655.1 Oryzias latipes sphingosine kinase 2 (LOC101160262), mRNA.

Price & Availability↑

CloneID	OJa25489
Clone ID Related Accession (Same CDS sequence)	XM_011485353.3 , XM_011485353.2
Accession Version	XM_011485353.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2661bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1491408000000
Organism	Oryzias latipes(Japanese medaka)
Product	sphingosine kinase 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_019874.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 7, 2017 this sequence version replaced XM_011485353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryzias latipes Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATCCC CTGAACCGTC CTCGTCGCCT CCAGCAGAAG CCCTGCTTCA TGGCCAGTTT 
GCTAGCTGGG GGTCAGGCAC CAACAGCAAC AGTAACAGCC GTCCCAGCAG CCCCGGCGGC 
CTCGGAGGAC TTTCCCCTGC TGCATCCCCA ACATCTCCTC CAGTGTCCAA CTATGCCTTG 
ACTCTCACGC ACAGCCACAT TCACATTCAG AGGCTGTCCC CAAAGTCAGG GAAGGAAGCC 
CGACTGCTGC TGCCTCTGTC TGAGCTGGTG GGGTGCAGCT GCCCCCGCTC CCCTGCACCC 
CCACTGTTTG TGTTGTACTG GTACCCCCCA GGGAAACGAA GGAAAGGGGT GTCCCGTCGC 
AGGCAGGTGA GGGCCTATCT AGCTGAAAGC AGGCCTGAAG CTGAAAGGTG GTCTGCTGCT 
GTACAGTGTC TCCTCAGAGG GGTGACTGTT ACTGCTGACA CCGAATTTTC GAGAAGTCTT 
CTACCTCGTC CCAGGCGGCT GCTGTTATTG GTGAATCCAT TCAGTGGAAG GGGCCAAGCA 
ATGCAATGGT GTCAGACCCA CATCCTGCCA ATGATTAGAG AGGCCAATAT CAGCTACAAC 
CTCATACAGA CTGAACGGCA GAATCATGCA AGAGAGCTCA TCAGAGAAGT GTCCCTCTCT 
GACTGGGATG GCATCGTCAT TGTCTCTGGA GATGGCTTGT TGCATGAGGT AATTAATGGT 
TTAATGGAGC GTCCAGACTG GGAACAAGCA ATAAAAACAC CTGTCGGCAT TCTGCCCTGC 
GGTTCCGGGA ATGCGCTGGC TGGTTCCATC AATCACAACG CAGGGTATGA CATGTGCCTT 
CGAGAGCCCC TCCTATTGAA CTGCTGCTTT CTGCTATGTC GAGGCGGGGT GCGACCGATG 
GACGTGGTCT CCGTGACAAC GAGCCCTGCT CCGTCCAACA ACAGTCATCC TGCAGCACCC 
AGGAGAGTCT TTTCCTTTCT TTCTGTTGCC TGGGGCTTTG TGTCTGATGT AGACATTGAA 
AGTGAGAGAT ATCGTGGCTT GGGCTCAGCC CGCTTCACCC TGGGCACTTT AGTTCGCATA 
GCTTCGCTCC GATCCTACAA AGGCCGCCTG TCCTTTCTGC CTCCCTCTGT CGTCCCTTCA 
TCCTCAGATG GCACGACCCC CCCGCCGAGG AGACCCCTGT CCCGCAGCAT CACTGAAGGC 
CTGGAGGGGT TTTGCCGATT GCCCATACAC CGCACCTGCT CTGACATGGG CATCAGCGAG 
CAAAGAAGTC TGCGGAGGGG TGAAGGAGAA AGGGAAAAGG AGGAGAGGCA GAGGGAAAGG 
GAGAGGAGAA GGGAGAGGGC CAGAGGGGGA GGAACTGGTG TGGTGAGGGC GAGCAGCCTT 
GCGGAGGACA GGGAAAAGGA GGGGGAGCTG GAGGCAGAGG ATGACAAGTC AAGGACTTGT 
TCAGAAAGGT CCGGGACAAG TTCTGAATCG AATGAAATGA ATAAAGAAAG GTTGGAGAAA 
ATTGAAGACG AGGGAACATT TGGACAAGAG TCTGTTGAGA TGGAGGAGGA TAGAGGGCAG 
GATGGAGGAG GGAGTGGCGA CTCTGTTGAA GAAGAGGAAG CGTTAGCCGC AAGAGAGCTA 
GATGAGGGCT ACGGGGTGGA CATGGGGCAA GAGGCGGATG AAGAAACAGA GGAGTCCATC 
ACTTACCCAG ACGACCCTCA GCGTGCCAGA CAGGGCGTTA GGAAGAATTC AGCCCCGTCC 
AGTGAGATCT GTAACGACCT GTCGAGTCAG CCTCTGCATA AGGTGGCTGA CGCAGATTCA 
AGCACAGCAG ATGGGGTGGA GGACATAGAT CTGAATGGAA CCTACTTCCA GAAAGATCCT 
TACACAGATG TGGCTCGGGA GCGATCTCTG ACCATCTCCT CTCCTTTTCG TCATTCTCCC 
TTTTCTTTCA AGCATAAAGC CCTGGACCAA AACCAAAACG CTTCCCGTCC AAGACCTCTG 
TCACTCCTGC AGCACTCCCA CACTAACTCT CTCCCCCCTA AAATGCCTTC CCTTTCACTG 
TCTCCTACTC CCCCGCCCTC CCCCTCTTGC GCCTCTCCAC ACTCATCCTA TTACCTTAAC 
CCTCGTCCAA ACACGCCAAA TTCCTCCTCG CCCTCTCCGT CTGTCCGCAC ACCGTCCTCA 
TCTTTCAACT TTGACATTTC CGAAGCAGCC AGACCCTTCA AGAACCGCCC GTTGGTCACG 
TTGCCTCCAG ATCTACCGAG AGACAACTTG TTACCCCCTC TTGACCAACC CCTCCCCACT 
AGAGACTGGG TGACCATCGA AGGGGACTTT GTGTTGGTCT TGGCCCTTTA TCAAACCCAC 
CTGGGAGCTG ACCTTCACGC CGCACCTCAA GCCAAGTTTG ACGACGGGCT CATTCACCTG 
ACATTTGTGC GGGCAGGAAT CTCCAGAGCC ACGCTACTAA GATTGTTCTT TGCCATGGAG 
AGAGGAACCC ACCACTCCGT CAGCTCGCCG TACGTGAGCC ACGTTACCTG CAAGGCCTTC 
AGGCTGCAAC CTCTATCTGC AAAAGGAACG CTCACCGTCG ATGGAGAACT TGTTCCTTAT 
GGGCCACTCC AGGCACAGGT TCATCCTTCC ATGGCTCGTC TCATTGTTGG AGACTACGGA 
GTGAAGATTA CCAGCTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011483655.1
CDS	1193..3853
Translation	MRSPEPSSSPPAEALLHGQFASWGSGTNSNSNSRPSSPGGLGGLSPAASPTSPPVSNYALTLTHSHIHIQRLSPKSGKEARLLLPLSELVGCSCPRSPAPPLFVLYWYPPGKRRKGVSRRRQVRAYLAESRPEAERWSAAVQCLLRGVTVTADTEFSRSLLPRPRRLLLLVNPFSGRGQAMQWCQTHILPMIREANISYNLIQTERQNHARELIREVSLSDWDGIVIVSGDGLLHEVINGLMERPDWEQAIKTPVGILPCGSGNALAGSINHNAGYDMCLREPLLLNCCFLLCRGGVRPMDVVSVTTSPAPSNNSHPAAPRRVFSFLSVAWGFVSDVDIESERYRGLGSARFTLGTLVRIASLRSYKGRLSFLPPSVVPSSSDGTTPPPRRPLSRSITEGLEGFCRLPIHRTCSDMGISEQRSLRRGEGEREKEERQRERERRRERARGGGTGVVRASSLAEDREKEGELEAEDDKSRTCSERSGTSSESNEMNKERLEKIEDEGTFGQESVEMEEDRGQDGGGSGDSVEEEEALAARELDEGYGVDMGQEADEETEESITYPDDPQRARQGVRKNSAPSSEICNDLSSQPLHKVADADSSTADGVEDIDLNGTYFQKDPYTDVARERSLTISSPFRHSPFSFKHKALDQNQNASRPRPLSLLQHSHTNSLPPKMPSLSLSPTPPPSPSCASPHSSYYLNPRPNTPNSSSPSPSVRTPSSSFNFDISEAARPFKNRPLVTLPPDLPRDNLLPPLDQPLPTRDWVTIEGDFVLVLALYQTHLGADLHAAPQAKFDDGLIHLTFVRAGISRATLLRLFFAMERGTHHSVSSPYVSHVTCKAFRLQPLSAKGTLTVDGELVPYGPLQAQVHPSMARLIVGDYGVKITSF

Target ORF information:

RefSeq Version	XM_011485353.2
Organism	Oryzias latipes(Japanese medaka)
Definition	Oryzias latipes sphingosine kinase 2 (LOC101160262), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011485353.2

ORF Insert Sequence:

ATGCGATCCC CTGAACCGTC CTCGTCGCCT CCAGCAGAAG CCCTGCTTCA TGGCCAGTTT 
GCTAGCTGGG GGTCAGGCAC CAACAGCAAC AGTAACAGCC GTCCCAGCAG CCCCGGCGGC 
CTCGGAGGAC TTTCCCCTGC TGCATCCCCA ACATCTCCTC CAGTGTCCAA CTATGCCTTG 
ACTCTCACGC ACAGCCACAT TCACATTCAG AGGCTGTCCC CAAAGTCAGG GAAGGAAGCC 
CGACTGCTGC TGCCTCTGTC TGAGCTGGTG GGGTGCAGCT GCCCCCGCTC CCCTGCACCC 
CCACTGTTTG TGTTGTACTG GTACCCCCCA GGGAAACGAA GGAAAGGGGT GTCCCGTCGC 
AGGCAGGTGA GGGCCTATCT AGCTGAAAGC AGGCCTGAAG CTGAAAGGTG GTCTGCTGCT 
GTACAGTGTC TCCTCAGAGG GGTGACTGTT ACTGCTGACA CCGAATTTTC GAGAAGTCTT 
CTACCTCGTC CCAGGCGGCT GCTGTTATTG GTGAATCCAT TCAGTGGAAG GGGCCAAGCA 
ATGCAATGGT GTCAGACCCA CATCCTGCCA ATGATTAGAG AGGCCAATAT CAGCTACAAC 
CTCATACAGA CTGAACGGCA GAATCATGCA AGAGAGCTCA TCAGAGAAGT GTCCCTCTCT 
GACTGGGATG GCATCGTCAT TGTCTCTGGA GATGGCTTGT TGCATGAGGT AATTAATGGT 
TTAATGGAGC GTCCAGACTG GGAACAAGCA ATAAAAACAC CTGTCGGCAT TCTGCCCTGC 
GGTTCCGGGA ATGCGCTGGC TGGTTCCATC AATCACAACG CAGGGTATGA CATGTGCCTT 
CGAGAGCCCC TCCTATTGAA CTGCTGCTTT CTGCTATGTC GAGGCGGGGT GCGACCGATG 
GACGTGGTCT CCGTGACAAC GAGCCCTGCT CCGTCCAACA ACAGTCATCC TGCAGCACCC 
AGGAGAGTCT TTTCCTTTCT TTCTGTTGCC TGGGGCTTTG TGTCTGATGT AGACATTGAA 
AGTGAGAGAT ATCGTGGCTT GGGCTCAGCC CGCTTCACCC TGGGCACTTT AGTTCGCATA 
GCTTCGCTCC GATCCTACAA AGGCCGCCTG TCCTTTCTGC CTCCCTCTGT CGTCCCTTCA 
TCCTCAGATG GCACGACCCC CCCGCCGAGG AGACCCCTGT CCCGCAGCAT CACTGAAGGC 
CTGGAGGGGT TTTGCCGATT GCCCATACAC CGCACCTGCT CTGACATGGG CATCAGCGAG 
CAAAGAAGTC TGCGGAGGGG TGAAGGAGAA AGGGAAAAGG AGGAGAGGCA GAGGGAAAGG 
GAGAGGAGAA GGGAGAGGGC CAGAGGGGGA GGAACTGGTG TGGTGAGGGC GAGCAGCCTT 
GCGGAGGACA GGGAAAAGGA GGGGGAGCTG GAGGCAGAGG ATGACAAGTC AAGGACTTGT 
TCAGAAAGGT CCGGGACAAG TTCTGAATCG AATGAAATGA ATAAAGAAAG GTTGGAGAAA 
ATTGAAGACG AGGGAACATT TGGACAAGAG TCTGTTGAGA TGGAGGAGGA TAGAGGGCAG 
GATGGAGGAG GGAGTGGCGA CTCTGTTGAA GAAGAGGAAG CGTTAGCCGC AAGAGAGCTA 
GATGAGGGCT ACGGGGTGGA CATGGGGCAA GAGGCGGATG AAGAAACAGA GGAGTCCATC 
ACTTACCCAG ACGACCCTCA GCGTGCCAGA CAGGGCGTTA GGAAGAATTC AGCCCCGTCC 
AGTGAGATCT GTAACGACCT GTCGAGTCAG CCTCTGCATA AGGTGGCTGA CGCAGATTCA 
AGCACAGCAG ATGGGGTGGA GGACATAGAT CTGAATGGAA CCTACTTCCA GAAAGATCCT 
TACACAGATG TGGCTCGGGA GCGATCTCTG ACCATCTCCT CTCCTTTTCG TCATTCTCCC 
TTTTCTTTCA AGCATAAAGC CCTGGACCAA AACCAAAACG CTTCCCGTCC AAGACCTCTG 
TCACTCCTGC AGCACTCCCA CACTAACTCT CTCCCCCCTA AAATGCCTTC CCTTTCACTG 
TCTCCTACTC CCCCGCCCTC CCCCTCTTGC GCCTCTCCAC ACTCATCCTA TTACCTTAAC 
CCTCGTCCAA ACACGCCAAA TTCCTCCTCG CCCTCTCCGT CTGTCCGCAC ACCGTCCTCA 
TCTTTCAACT TTGACATTTC CGAAGCAGCC AGACCCTTCA AGAACCGCCC GTTGGTCACG 
TTGCCTCCAG ATCTACCGAG AGACAACTTG TTACCCCCTC TTGACCAACC CCTCCCCACT 
AGAGACTGGG TGACCATCGA AGGGGACTTT GTGTTGGTCT TGGCCCTTTA TCAAACCCAC 
CTGGGAGCTG ACCTTCACGC CGCACCTCAA GCCAAGTTTG ACGACGGGCT CATTCACCTG 
ACATTTGTGC GGGCAGGAAT CTCCAGAGCC ACGCTACTAA GATTGTTCTT TGCCATGGAG 
AGAGGAACCC ACCACTCCGT CAGCTCGCCG TACGTGAGCC ACGTTACCTG CAAGGCCTTC 
AGGCTGCAAC CTCTATCTGC AAAAGGAACG CTCACCGTCG ATGGAGAACT TGTTCCTTAT 
GGGCCACTCC AGGCACAGGT TCATCCTTCC ATGGCTCGTC TCATTGTTGG AGACTACGGA 
GTGAAGATTA CCAGCTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC101160262 cDNA ORF clone, Oryzias latipes(Japanese medaka)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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