SPEF2 cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPEF2
Entrez Gene ID	100353102
Full Name	sperm flagellar 2
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008262114.2	XP_008260336.1	sperm flagellar protein 2 isoform X4
XM_008262116.2	XP_008260338.1	sperm flagellar protein 2 isoform X5
XM_017344675.1	XP_017200164.1	sperm flagellar protein 2 isoform X2
XM_017344676.1	XP_017200165.1	sperm flagellar protein 2 isoform X3
XM_008262111.2	XP_008260333.1	sperm flagellar protein 2 isoform X1

Pathways from BioSystems
Gene Ontology

The following SPEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOb11242	XM_008262114.2 Latest version!	Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOb11244	XM_008262116.2 Latest version!	Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOb11239	XM_008262111.2 Latest version!	Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOb35039	XM_017344675.1 Latest version!	Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOb35040	XM_017344676.1 Latest version!	Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPEF2 ( XM_008262114.2 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_008260336.1 Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OOb11242
Clone ID Related Accession (Same CDS sequence)	XM_008262114.2
Accession Version	XM_008262114.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5271bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	sperm flagellar protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013679.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2016 this sequence version replaced XM_008262114.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCCCG GACCGTGAGC 
CCCAAGTCAT TTGCAAAGGC ATTTTCCACT GGCTATCTAA TTGGAGAAGT TCTGCACAAG 
TTTGAACTCC AGGGTGACTT CTCAGAATTT TCAGAAAGCA GGACTTCAAC TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTG CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCAAA GCATCATCAC AGAACAGCCT GGAGCGGCCA CAAAACTTTT ATATCAATTG 
TACATTGCTC TTCAGAGAAA AAAGAAAAGT GGCCTCACTG GACTAGAAAT GCAAACCATG 
CAGCCCCTGA CAAATTTAAG ACTTCAGAAC ATGAGAAGTG AGGCTTTTCG AGAGAGACTT 
AAAATCCTGA TACCACGTCA AACTGATTTC AATCTGATGC GGGTTACATA CAGGTTCCAA 
GAAAAATATA GACGCATGAG AGAAGATGTT GACCGTGTGC ATTCTGAGAA ACTTGAAAGA 
ATGCAAAAAC TCAGCGAAGA GCAAAGATGT TTTAATATCG AAAAGCAACG CTTAAGCAGA 
AGACGACAAA ATGAAATAAT GGCCCAAATC CAAGCAGCTA TCATACAGAT TCCTAAACCA 
GCATCAAATC ACACTTTGAA AGCACTAGAG GCCCAGAAAA TGATGAAAAA GAAAAAAGGT 
GCAGAGGATG TGGCCAATGA GATTAAGAAG TTTGAAGCTT TAATAAAAAA GGATCTTCAG 
GCAAAAGAAA GTGCCTCCAA GACTTCTGTA GACACAACAG GTCCGATGAC TACCGACTTG 
TTGAACACTT ACTCGGATGA TGACTACATT AAAAAAATCC AGAAGCGCCT AGAAGAAGAT 
GCATTTGCAC GAGAACAAAG AGAGAAAAGG CGGCGGAGAC TGCTAATGGA CCAGTTAATA 
GCGCATGAAG CGCAAGAGGA GGCTTACCGG GAGGAGCAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATCGCAGTG CAGCTCATGC ACGTCCGACA TGAGAAGGAT 
GTCCTATGGC AAAACAGGAT CTTCAGAGAA AAGCAGTATG AGGAAAGGCG ACTTAAAGAT 
TTCCAGGATG CACTTGATCG AGAAGCGGCC TTGGCAAAAC AAGCCAAGAT TGATTTCGAA 
GAACAAATCC TTAGAGAGAA GGAAATTCAT GAGCAGATCG CTGTGGAAAG AGCTCAAGCT 
CGTTACAAAA AGCATTATGC AGTGTGTGCA GAAATCTTGG ATCAGATACT TGACTTGTCC 
ACGAAAGTGG CAGATTATCG TATGTTGACA AATAATCTGA TTCCACATAA GATGATGCAT 
GATTGGAAGG AGCTATTTTT TAATGGAAAA CCCATATATG AGCAAGCCTC CATTAAGCAG 
ATAGCTGCTA AACCCACTGA AGAACAACTT ATAGAACTGG AAAAAAGGGA TTTGCTAGAT 
AGCAATGACT ACGACGACTA CAAGAATATG GTTGGGGAGT GGGCCTTACC AGAAGAAATG 
GCTGAAAATT TACCACCTTC CAACAATTGC ATATTAGGCC ATGTGCTTCA CAGACTAGTT 
GAAAAATCTC TTCCTCCTCA AGTTGAATCA ACAGAACCTG AATTACTTTC ATCTTCTGTT 
AAGGGATGCG TATTGGGAAA AACATTTAGT GGGAAAACTA CCACTCTAAA GTCTCTACAA 
AAAGACTTCC CTGTTAAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TATCATGACA ACGAAAAAGT CAGTGAGGCC TCATCCATTC CTCAAGAGGA TAGGAAAGAA 
GATCAGCTCA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AACAATTGCT GAAGAAAGGA 
AAGAGCATTC CAGACATGTT GCTGGTTAAC ATCATGGTAA ACGCTATTAA TGGGATACCT 
GTGAATCAGA ACTGGATCCT TGATGGTTTT CCAATGACAT TAAACCAAGC TCAACTTCTG 
GAGGAAACTC TCACAGGCTG CAACAGATAC CTCATGGAAG CAGAAGGAAA AGCAGCACAA 
ATATCTACAC TGGCTGTGGA TCCTACAACT TCCAAAGAAG TACCTCTGCC CTCTTCTGCA 
TTTGATTTTG TTATACTATT AGATATTTCT GATAATTCCT CACTGAGTCG CATGAATGAT 
GTCATGGCGG AAGAATTTTC ACGTGTAATT TCTCATGAAG ATACCAGTGA GCATGCAGCT 
GCTGAGAGCC AGCATGTAGA TGTGGATCCT AATTTGAGAG ACCAGATACA ACACAGGATT 
ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT 
TTGATAAGAA TCAATGCTGA AATAGATAAA AACTCTTTAT GTCAAAAAGT AAAAGAAATA 
TTTGCAACTG AAATAATGAA AAAAAAGAAT AAAGTTGAGA AAAGGTTAGA AGAAAAGGAA 
GCTGAGAAAA AAGCAGCTTC GCTGGCTGAG CCTCCACCCC CTACTCCTCC TCTTCCTCCT 
GAGCCAGAAA AAGAGAAGGA AACTCATCAG CAGCGGGACC CTTCAAAAAC GCCCCCTGCA 
AAAGGAAAAC CACAATCAGA AACTTCACCT GGTAAGCAAG ACTCTCTTCA GGATGGAAAA 
GGAAAGAAAG GTTCTCCTAA AGGAAAACCC CCAGGAGGAA AAGTCCAAGT GAAGAAATCA 
CCTGTTGGCT CTACAGATAT ACCACCTACT CCAGTAGCAC CACCGCCCCC TAAGCCAGGA 
TCTGAAGAAT GGGTCTATGT GAATGAACCA ATTCCTGAGG AAATACCTTC ATTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCTTAC ATAAACACTA TCAAAACCAT CCTCAGGCAT 
CTGAGAGAAG ACCAGCATGA AGTACTTAAA TACTTATATG AAATTACAAC AAGTTTCCAG 
GAGTTTCTAA GGCGTCCAGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCTTTTC CTGATGACTT GTGGGAAGAT GAGGAAACCA AGGCTGAACT ACACCAAAGA 
GTGAACGATT TACGAGACCG CCTGTGGGAC ATTTGTGATG CCAGGAAGGA AGAGGCTGAG 
CAGGAACGGC TGGATATCAT TAACGAAAGC TGGCTGCAGG ACTCCCTTGG AATCACGATG 
AACCACTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC AAAGAAACTC 
CTGCAAGATT ATTATAGGGC AATGGAACGC AAAATCCCAA TAGATGACAG CAAGAGATTT 
ACTCGAATCC CGTTGATCCG ACTGGATAGT AAAGATATTT CAGAAAACCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCCA GAAACAGGCG TGCCCAGACC AAAATTAAAA 
GCAATACTGA AAGGCAAGAT TGATTACTCA CTAGAAAACG TGCAGACAAA CTTTGAGGCA 
GATGAGAGGT TGGTAATGGA CACCTGGCAG CAGGCTTCCT TAGCGATATC TAACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTCATAGAA GAAGACAAAG AAAACCAACC AGCAGAGTCA 
AAAGAGAAAT CTCCCCAGAT GAGTACAAAC AAAAAAGGCA AAAAGGAAAA TGAGCCACCC 
AAGAATAAAA AGGAAGACAA ACATGGAAAA GGTAAGTCAC CACCTGTAGC TGAAGCTGCT 
CCTGTAGTGT TCACAGCAGA GGAAATCGCA GAAATTGAAA AGAAAAATGA ACTGAGGCTC 
AGAATAAAGG AGGAGCATCT TGCCGCCCTA CAGTTTGAAG AGATAGCTAC ACAGTTTCGA 
CTTGAATTGA TAAAATCCAA AGCATTGTCT GTCCTTGAAG ACCTAATAAC GAAGGTGGTC 
GATATATATA AACTCATGGA AAAATGGCTT GGTGAGAGAT ATTTGAATGA AATGTCCAGT 
GTAGAAAAAT TAACAGAAGT AGCTCGCTAT CACATTGAAA CAGCTACAAA AATCCAGAAT 
GAACTTTACT TAAACCAAGA AGACTTCTTC ATTAATGGTG ATATAAAAGT CTTCCCAGAT 
CCACCCCTGC CGAAACGTCC TCCACCTGTA GAAAAGGAAG AAGACGGCAC CCTGACTATT 
GAGCAGCTCG ACAATCTTCG AGATCAGTTC TTAGATATGG CGCCTAAAGG CATAATAGGA 
AGTAAAGCAT TTACTGACAT TCTGCTTGAT TTGATGACCT TGAACCTTGG AACAAACAAC 
TTCCCTAGTA GCTGGATGCA CCTCACCCAG CCTGAATTAC AGGAGTTGAC ATACTTGTTG 
ATAGTAAACT CAGAGTTTGT GGACTGGAGG AAATTCCTGT TAGTAACCGC ATTGCCTTGG 
CCCATCCCTC TGGAAGAAGA GCTCCTGGAG ACCCTGCAGA GGTTCAAGGC TGTGGATGAG 
GAGCAGATGG GCACCGTCAC TTTTGAGCAG TATATGCAGG CTGGCCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TGCCATATAA TAGGCAGGTT 
CATCTAATAG AGTTTTTCTT TAGGTTATTT GCTGACTATG AGATGGATCC ACCCCAGCTT 
GACTACACAC AAATGCTGCT GTATTTTGCT TGCCACCCAG ACCCTGTGGA AGGGGTCTAC 
AGGGCCCTCA GTGTGGCCAT TGGAACTCAT ATCTTCCGGA AAGTTGAAGG TCCAGTTATG 
ACAGCAGAAA AGACCTCCTC TACTGATTTG AGTCCCGTCG AGGGATATGT GGAAACTGAG 
GAAAATGCTG AAAGAGAGAA AAGGGAGTTC ACAGAGGAAA AGGAGGAAAG GGATCAGAAG 
GAAGTGGATA TCCTGGAAAA TGCAAACACT GAAAAGATTT CCATGGAAAC ACTACTCAGA 
GTGTTCCGAG GAGGAAGCGA AGTGAAGGAT GCGAACAGAT TTACCAGCCA CCTTAAGAGA 
GAGAACATTT ATGCAGAGAG CTTCATGAAA GTATTTCAAG AACTAGAGAC CAAGGACCTG 
GAGCCAATTG AAGTTGCTGT TCTCTTGAAG CATCCTTTCA TCCAAGACCT GATTGCAAAT 
TATCCAGACT ATAAAATCCC TGATATTAAA ATGATTCTCC AAAGGAGTGT ACATGTAGCT 
GGAAGTGATG GAGAGAGATC ACCCTCAAGA CTTACAGAGG AAAAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008260336.1
CDS	225..5495
Translation	MSEILCQWLNQELKVSRTVSPKSFAKAFSTGYLIGEVLHKFELQGDFSEFSESRTSTAKLNNFSRLEPTLHLLGVQFDQNVAQSIITEQPGAATKLLYQLYIALQRKKKSGLTGLEMQTMQPLTNLRLQNMRSEAFRERLKILIPRQTDFNLMRVTYRFQEKYRRMREDVDRVHSEKLERMQKLSEEQRCFNIEKQRLSRRRQNEIMAQIQAAIIQIPKPASNHTLKALEAQKMMKKKKGAEDVANEIKKFEALIKKDLQAKESASKTSVDTTGPMTTDLLNTYSDDDYIKKIQKRLEEDAFAREQREKRRRRLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKDVLWQNRIFREKQYEERRLKDFQDALDREAALAKQAKIDFEEQILREKEIHEQIAVERAQARYKKHYAVCAEILDQILDLSTKVADYRMLTNNLIPHKMMHDWKELFFNGKPIYEQASIKQIAAKPTEEQLIELEKRDLLDSNDYDDYKNMVGEWALPEEMAENLPPSNNCILGHVLHRLVEKSLPPQVESTEPELLSSSVKGCVLGKTFSGKTTTLKSLQKDFPVKILSIDTLVQEAIQAYHDNEKVSEASSIPQEDRKEDQLTVRAQLGAKSEQLLKKGKSIPDMLLVNIMVNAINGIPVNQNWILDGFPMTLNQAQLLEETLTGCNRYLMEAEGKAAQISTLAVDPTTSKEVPLPSSAFDFVILLDISDNSSLSRMNDVMAEEFSRVISHEDTSEHAAAESQHVDVDPNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIRINAEIDKNSLCQKVKEIFATEIMKKKNKVEKRLEEKEAEKKAASLAEPPPPTPPLPPEPEKEKETHQQRDPSKTPPAKGKPQSETSPGKQDSLQDGKGKKGSPKGKPPGGKVQVKKSPVGSTDIPPTPVAPPPPKPGSEEWVYVNEPIPEEIPSFLVPYWELIENSYINTIKTILRHLREDQHEVLKYLYEITTSFQEFLRRPDHKQDFVAQWQADFNSFPDDLWEDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDSLGITMNHFFSLMQAELNRFQDTKKLLQDYYRAMERKIPIDDSKRFTRIPLIRLDSKDISENQLRIPLVPRISISPETGVPRPKLKAILKGKIDYSLENVQTNFEADERLVMDTWQQASLAISNMVAAEIHQRLIEEDKENQPAESKEKSPQMSTNKKGKKENEPPKNKKEDKHGKGKSPPVAEAAPVVFTAEEIAEIEKKNELRLRIKEEHLAALQFEEIATQFRLELIKSKALSVLEDLITKVVDIYKLMEKWLGERYLNEMSSVEKLTEVARYHIETATKIQNELYLNQEDFFINGDIKVFPDPPLPKRPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGIIGSKAFTDILLDLMTLNLGTNNFPSSWMHLTQPELQELTYLLIVNSEFVDWRKFLLVTALPWPIPLEEELLETLQRFKAVDEEQMGTVTFEQYMQAGLWFTGDEDIKIPENPLEPLPYNRQVHLIEFFFRLFADYEMDPPQLDYTQMLLYFACHPDPVEGVYRALSVAIGTHIFRKVEGPVMTAEKTSSTDLSPVEGYVETEENAEREKREFTEEKEERDQKEVDILENANTEKISMETLLRVFRGGSEVKDANRFTSHLKRENIYAESFMKVFQELETKDLEPIEVAVLLKHPFIQDLIANYPDYKIPDIKMILQRSVHVAGSDGERSPSRLTEEKK

Target ORF information:

RefSeq Version	XM_008262114.2
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008262114.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCCCG GACCGTGAGC 
CCCAAGTCAT TTGCAAAGGC ATTTTCCACT GGCTATCTAA TTGGAGAAGT TCTGCACAAG 
TTTGAACTCC AGGGTGACTT CTCAGAATTT TCAGAAAGCA GGACTTCAAC TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTG CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCAAA GCATCATCAC AGAACAGCCT GGAGCGGCCA CAAAACTTTT ATATCAATTG 
TACATTGCTC TTCAGAGAAA AAAGAAAAGT GGCCTCACTG GACTAGAAAT GCAAACCATG 
CAGCCCCTGA CAAATTTAAG ACTTCAGAAC ATGAGAAGTG AGGCTTTTCG AGAGAGACTT 
AAAATCCTGA TACCACGTCA AACTGATTTC AATCTGATGC GGGTTACATA CAGGTTCCAA 
GAAAAATATA GACGCATGAG AGAAGATGTT GACCGTGTGC ATTCTGAGAA ACTTGAAAGA 
ATGCAAAAAC TCAGCGAAGA GCAAAGATGT TTTAATATCG AAAAGCAACG CTTAAGCAGA 
AGACGACAAA ATGAAATAAT GGCCCAAATC CAAGCAGCTA TCATACAGAT TCCTAAACCA 
GCATCAAATC ACACTTTGAA AGCACTAGAG GCCCAGAAAA TGATGAAAAA GAAAAAAGGT 
GCAGAGGATG TGGCCAATGA GATTAAGAAG TTTGAAGCTT TAATAAAAAA GGATCTTCAG 
GCAAAAGAAA GTGCCTCCAA GACTTCTGTA GACACAACAG GTCCGATGAC TACCGACTTG 
TTGAACACTT ACTCGGATGA TGACTACATT AAAAAAATCC AGAAGCGCCT AGAAGAAGAT 
GCATTTGCAC GAGAACAAAG AGAGAAAAGG CGGCGGAGAC TGCTAATGGA CCAGTTAATA 
GCGCATGAAG CGCAAGAGGA GGCTTACCGG GAGGAGCAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATCGCAGTG CAGCTCATGC ACGTCCGACA TGAGAAGGAT 
GTCCTATGGC AAAACAGGAT CTTCAGAGAA AAGCAGTATG AGGAAAGGCG ACTTAAAGAT 
TTCCAGGATG CACTTGATCG AGAAGCGGCC TTGGCAAAAC AAGCCAAGAT TGATTTCGAA 
GAACAAATCC TTAGAGAGAA GGAAATTCAT GAGCAGATCG CTGTGGAAAG AGCTCAAGCT 
CGTTACAAAA AGCATTATGC AGTGTGTGCA GAAATCTTGG ATCAGATACT TGACTTGTCC 
ACGAAAGTGG CAGATTATCG TATGTTGACA AATAATCTGA TTCCACATAA GATGATGCAT 
GATTGGAAGG AGCTATTTTT TAATGGAAAA CCCATATATG AGCAAGCCTC CATTAAGCAG 
ATAGCTGCTA AACCCACTGA AGAACAACTT ATAGAACTGG AAAAAAGGGA TTTGCTAGAT 
AGCAATGACT ACGACGACTA CAAGAATATG GTTGGGGAGT GGGCCTTACC AGAAGAAATG 
GCTGAAAATT TACCACCTTC CAACAATTGC ATATTAGGCC ATGTGCTTCA CAGACTAGTT 
GAAAAATCTC TTCCTCCTCA AGTTGAATCA ACAGAACCTG AATTACTTTC ATCTTCTGTT 
AAGGGATGCG TATTGGGAAA AACATTTAGT GGGAAAACTA CCACTCTAAA GTCTCTACAA 
AAAGACTTCC CTGTTAAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TATCATGACA ACGAAAAAGT CAGTGAGGCC TCATCCATTC CTCAAGAGGA TAGGAAAGAA 
GATCAGCTCA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AACAATTGCT GAAGAAAGGA 
AAGAGCATTC CAGACATGTT GCTGGTTAAC ATCATGGTAA ACGCTATTAA TGGGATACCT 
GTGAATCAGA ACTGGATCCT TGATGGTTTT CCAATGACAT TAAACCAAGC TCAACTTCTG 
GAGGAAACTC TCACAGGCTG CAACAGATAC CTCATGGAAG CAGAAGGAAA AGCAGCACAA 
ATATCTACAC TGGCTGTGGA TCCTACAACT TCCAAAGAAG TACCTCTGCC CTCTTCTGCA 
TTTGATTTTG TTATACTATT AGATATTTCT GATAATTCCT CACTGAGTCG CATGAATGAT 
GTCATGGCGG AAGAATTTTC ACGTGTAATT TCTCATGAAG ATACCAGTGA GCATGCAGCT 
GCTGAGAGCC AGCATGTAGA TGTGGATCCT AATTTGAGAG ACCAGATACA ACACAGGATT 
ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT 
TTGATAAGAA TCAATGCTGA AATAGATAAA AACTCTTTAT GTCAAAAAGT AAAAGAAATA 
TTTGCAACTG AAATAATGAA AAAAAAGAAT AAAGTTGAGA AAAGGTTAGA AGAAAAGGAA 
GCTGAGAAAA AAGCAGCTTC GCTGGCTGAG CCTCCACCCC CTACTCCTCC TCTTCCTCCT 
GAGCCAGAAA AAGAGAAGGA AACTCATCAG CAGCGGGACC CTTCAAAAAC GCCCCCTGCA 
AAAGGAAAAC CACAATCAGA AACTTCACCT GGTAAGCAAG ACTCTCTTCA GGATGGAAAA 
GGAAAGAAAG GTTCTCCTAA AGGAAAACCC CCAGGAGGAA AAGTCCAAGT GAAGAAATCA 
CCTGTTGGCT CTACAGATAT ACCACCTACT CCAGTAGCAC CACCGCCCCC TAAGCCAGGA 
TCTGAAGAAT GGGTCTATGT GAATGAACCA ATTCCTGAGG AAATACCTTC ATTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCTTAC ATAAACACTA TCAAAACCAT CCTCAGGCAT 
CTGAGAGAAG ACCAGCATGA AGTACTTAAA TACTTATATG AAATTACAAC AAGTTTCCAG 
GAGTTTCTAA GGCGTCCAGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCTTTTC CTGATGACTT GTGGGAAGAT GAGGAAACCA AGGCTGAACT ACACCAAAGA 
GTGAACGATT TACGAGACCG CCTGTGGGAC ATTTGTGATG CCAGGAAGGA AGAGGCTGAG 
CAGGAACGGC TGGATATCAT TAACGAAAGC TGGCTGCAGG ACTCCCTTGG AATCACGATG 
AACCACTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC AAAGAAACTC 
CTGCAAGATT ATTATAGGGC AATGGAACGC AAAATCCCAA TAGATGACAG CAAGAGATTT 
ACTCGAATCC CGTTGATCCG ACTGGATAGT AAAGATATTT CAGAAAACCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCCA GAAACAGGCG TGCCCAGACC AAAATTAAAA 
GCAATACTGA AAGGCAAGAT TGATTACTCA CTAGAAAACG TGCAGACAAA CTTTGAGGCA 
GATGAGAGGT TGGTAATGGA CACCTGGCAG CAGGCTTCCT TAGCGATATC TAACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTCATAGAA GAAGACAAAG AAAACCAACC AGCAGAGTCA 
AAAGAGAAAT CTCCCCAGAT GAGTACAAAC AAAAAAGGCA AAAAGGAAAA TGAGCCACCC 
AAGAATAAAA AGGAAGACAA ACATGGAAAA GGTAAGTCAC CACCTGTAGC TGAAGCTGCT 
CCTGTAGTGT TCACAGCAGA GGAAATCGCA GAAATTGAAA AGAAAAATGA ACTGAGGCTC 
AGAATAAAGG AGGAGCATCT TGCCGCCCTA CAGTTTGAAG AGATAGCTAC ACAGTTTCGA 
CTTGAATTGA TAAAATCCAA AGCATTGTCT GTCCTTGAAG ACCTAATAAC GAAGGTGGTC 
GATATATATA AACTCATGGA AAAATGGCTT GGTGAGAGAT ATTTGAATGA AATGTCCAGT 
GTAGAAAAAT TAACAGAAGT AGCTCGCTAT CACATTGAAA CAGCTACAAA AATCCAGAAT 
GAACTTTACT TAAACCAAGA AGACTTCTTC ATTAATGGTG ATATAAAAGT CTTCCCAGAT 
CCACCCCTGC CGAAACGTCC TCCACCTGTA GAAAAGGAAG AAGACGGCAC CCTGACTATT 
GAGCAGCTCG ACAATCTTCG AGATCAGTTC TTAGATATGG CGCCTAAAGG CATAATAGGA 
AGTAAAGCAT TTACTGACAT TCTGCTTGAT TTGATGACCT TGAACCTTGG AACAAACAAC 
TTCCCTAGTA GCTGGATGCA CCTCACCCAG CCTGAATTAC AGGAGTTGAC ATACTTGTTG 
ATAGTAAACT CAGAGTTTGT GGACTGGAGG AAATTCCTGT TAGTAACCGC ATTGCCTTGG 
CCCATCCCTC TGGAAGAAGA GCTCCTGGAG ACCCTGCAGA GGTTCAAGGC TGTGGATGAG 
GAGCAGATGG GCACCGTCAC TTTTGAGCAG TATATGCAGG CTGGCCTGTG GTTTACAGGA 
GATGAAGATA TAAAAATTCC AGAAAATCCT CTTGAACCCC TGCCATATAA TAGGCAGGTT 
CATCTAATAG AGTTTTTCTT TAGGTTATTT GCTGACTATG AGATGGATCC ACCCCAGCTT 
GACTACACAC AAATGCTGCT GTATTTTGCT TGCCACCCAG ACCCTGTGGA AGGGGTCTAC 
AGGGCCCTCA GTGTGGCCAT TGGAACTCAT ATCTTCCGGA AAGTTGAAGG TCCAGTTATG 
ACAGCAGAAA AGACCTCCTC TACTGATTTG AGTCCCGTCG AGGGATATGT GGAAACTGAG 
GAAAATGCTG AAAGAGAGAA AAGGGAGTTC ACAGAGGAAA AGGAGGAAAG GGATCAGAAG 
GAAGTGGATA TCCTGGAAAA TGCAAACACT GAAAAGATTT CCATGGAAAC ACTACTCAGA 
GTGTTCCGAG GAGGAAGCGA AGTGAAGGAT GCGAACAGAT TTACCAGCCA CCTTAAGAGA 
GAGAACATTT ATGCAGAGAG CTTCATGAAA GTATTTCAAG AACTAGAGAC CAAGGACCTG 
GAGCCAATTG AAGTTGCTGT TCTCTTGAAG CATCCTTTCA TCCAAGACCT GATTGCAAAT 
TATCCAGACT ATAAAATCCC TGATATTAAA ATGATTCTCC AAAGGAGTGT ACATGTAGCT 
GGAAGTGATG GAGAGAGATC ACCCTCAAGA CTTACAGAGG AAAAGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_008262116.2 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_008260338.1 Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OOb11244
Clone ID Related Accession (Same CDS sequence)	XM_008262116.2
Accession Version	XM_008262116.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	sperm flagellar protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013679.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2016 this sequence version replaced XM_008262116.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCCCG GACCGTGAGC 
CCCAAGTCAT TTGCAAAGGC ATTTTCCACT GGCTATCTAA TTGGAGAAGT TCTGCACAAG 
TTTGAACTCC AGGGTGACTT CTCAGAATTT TCAGAAAGCA GGACTTCAAC TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTG CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCAAA GCATCATCAC AGAACAGCCT GGAGCGGCCA CAAAACTTTT ATATCAATTG 
TACATTGCTC TTCAGAGAAA AAAGAAAAGT GGCCTCACTG GACTAGAAAT GCAAACCATG 
CAGCCCCTGA CAAATTTAAG ACTTCAGAAC ATGAGAAGTG AGGCTTTTCG AGAGCAACGC 
TTAAGCAGAA GACGACAAAA TGAAATAATG GCCCAAATCC AAGCAGCTAT CATACAGATT 
CCTAAACCAG CATCAAATCA CACTTTGAAA GCACTAGAGG CCCAGAAAAT GATGAAAAAG 
AAAAAAGGTG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG 
GATCTTCAGG CAAAAGAAAG TGCCTCCAAG ACTTCTGTAG ACACAACAGG TCCGATGACT 
ACCGACTTGT TGAACACTTA CTCGGATGAT GACTACATTA AAAAAATCCA GAAGCGCCTA 
GAAGAAGATG CATTTGCACG AGAACAAAGA GAGAAAAGGC GGCGGAGACT GCTAATGGAC 
CAGTTAATAG CGCATGAAGC GCAAGAGGAG GCTTACCGGG AGGAGCAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCGCAGTGC AGCTCATGCA CGTCCGACAT 
GAGAAGGATG TCCTATGGCA AAACAGGATC TTCAGAGAAA AGCAGTATGA GGAAAGGCGA 
CTTAAAGATT TCCAGGATGC ACTTGATCGA GAAGCGGCCT TGGCAAAACA AGCCAAGATT 
GATTTCGAAG AACAAATCCT TAGAGAGAAG GAAATTCATG AGCAGATCGC TGTGGAAAGA 
GCTCAAGCTC GTTACAAAAA GCATTATGCA GTGTGTGCAG AAATCTTGGA TCAGATACTT 
GACTTGTCCA CGAAAGTGGC AGATTATCGT ATGTTGACAA ATAATCTGAT TCCACATAAG 
ATGATGCATG ATTGGAAGGA GCTATTTTTT AATGGAAAAC CCATATATGA GCAAGCCTCC 
ATTAAGCAGA TAGCTGCTAA ACCCACTGAA GAACAACTTA TAGAACTGGA AAAAAGGGAT 
TTGCTAGATA GCAATGACTA CGACGACTAC AAGAATATGG TTGGGGAGTG GGCCTTACCA 
GAAGAAATGG CTGAAAATTT ACCACCTTCC AACAATTGCA TATTAGGCCA TGTGCTTCAC 
AGACTAGTTG AAAAATCTCT TCCTCCTCAA GTTGAATCAA CAGAACCTGA ATTACTTTCA 
TCTTCTGTTA AGGGATGCGT ATTGGGAAAA ACATTTAGTG GGAAAACTAC CACTCTAAAG 
TCTCTACAAA AAGACTTCCC TGTTAAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT ATCATGACAA CGAAAAAGTC AGTGAGGCCT CATCCATTCC TCAAGAGGAT 
AGGAAAGAAG ATCAGCTCAC TGTGCGGGCT CAGCTTGGTG CAAAATCAGA ACAATTGCTG 
AAGAAAGGAA AGAGCATTCC AGACATGTTG CTGGTTAACA TCATGGTAAA CGCTATTAAT 
GGGATACCTG TGAATCAGAA CTGGATCCTT GATGGTTTTC CAATGACATT AAACCAAGCT 
CAACTTCTGG AGGAAACTCT CACAGGCTGC AACAGATACC TCATGGAAGC AGAAGGAAAA 
GCAGCACAAA TATCTACACT GGCTGTGGAT CCTACAACTT CCAAAGAAGT ACCTCTGCCC 
TCTTCTGCAT TTGATTTTGT TATACTATTA GATATTTCTG ATAATTCCTC ACTGAGTCGC 
ATGAATGATG TCATGGCGGA AGAATTTTCA CGTGTAATTT CTCATGAAGA TACCAGTGAG 
CATGCAGCTG CTGAGAGCCA GCATGTAGAT GTGGATCCTA ATTTGAGAGA CCAGATACAA 
CACAGGATTA TAGGCTTCTT GGACAACTGG CCTTTATTGG AGCAATGGTT TTCAGAGCCA 
GAAAATATTT TGATAAGAAT CAATGCTGAA ATAGATAAAA ACTCTTTATG TCAAAAAGTA 
AAAGAAATAT TTGCAACTGA AATAATGAAA AAAAAGAATA AAGTTGAGAA AAGGTTAGAA 
GAAAAGGAAG CTGAGAAAAA AGCAGCTTCG CTGGCTGAGC CTCCACCCCC TACTCCTCCT 
CTTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACTCATCAGC AGCGGGACCC TTCAAAAACG 
CCCCCTGCAA AAGGAAAACC ACAATCAGAA ACTTCACCTG GTAAGCAAGA CTCTCTTCAG 
GATGGAAAAG GAAAGAAAGG TTCTCCTAAA GGAAAACCCC CAGGAGGAAA AGTCCAAGTG 
AAGAAATCAC CTGTTGGCTC TACAGATATA CCACCTACTC CAGTAGCACC ACCGCCCCCT 
AAGCCAGGAT CTGAAGAATG GGTCTATGTG AATGAACCAA TTCCTGAGGA AATACCTTCA 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCTTACA TAAACACTAT CAAAACCATC 
CTCAGGCATC TGAGAGAAGA CCAGCATGAA GTACTTAAAT ACTTATATGA AATTACAACA 
AGTTTCCAGG AGTTTCTAAG GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCTTTTCC TGATGACTTG TGGGAAGATG AGGAAACCAA GGCTGAACTA 
CACCAAAGAG TGAACGATTT ACGAGACCGC CTGTGGGACA TTTGTGATGC CAGGAAGGAA 
GAGGCTGAGC AGGAACGGCT GGATATCATT AACGAAAGCT GGCTGCAGGA CTCCCTTGGA 
ATCACGATGA ACCACTTCTT CTCACTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACA 
AAGAAACTCC TGCAAGATTA TTATAGGGCA ATGGAACGCA AAATCCCAAT AGATGACAGC 
AAGAGATTTA CTCGAATCCC GTTGATCCGA CTGGATAGTA AAGATATTTC AGAAAACCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCCAG AAACAGGCGT GCCCAGACCA 
AAATTAAAAG CAATACTGAA AGGCAAGATT GATTACTCAC TAGAAAACGT GCAGACAAAC 
TTTGAGGCAG ATGAGAGGTT GGTAATGGAC ACCTGGCAGC AGGCTTCCTT AGCGATATCT 
AACATGGTGG CTGCTGAAAT TCATCAGAGG CTCATAGAAG AAGACAAAGA AAACCAACCA 
GCAGAGTCAA AAGAGAAATC TCCCCAGATG AGTACAAACA AAAAAGGCAA AAAGGAAAAT 
GAGCCACCCA AGAATAAAAA GGAAGACAAA CATGGAAAAG GTAAGTCACC ACCTGTAGCT 
GAAGCTGCTC CTGTAGTGTT CACAGCAGAG GAAATCGCAG AAATTGAAAA GAAAAATGAA 
CTGAGGCTCA GAATAAAGGA GGAGCATCTT GCCGCCCTAC AGTTTGAAGA GATAGCTACA 
CAGTTTCGAC TTGAATTGAT AAAATCCAAA GCATTGTCTG TCCTTGAAGA CCTAATAACG 
AAGGTGGTCG ATATATATAA ACTCATGGAA AAATGGCTTG GTGAGAGATA TTTGAATGAA 
ATGTCCAGTG TAGAAAAATT AACAGAAGTA GCTCGCTATC ACATTGAAAC AGCTACAAAA 
ATCCAGAATG AACTTTACTT AAACCAAGAA GACTTCTTCA TTAATGGTGA TATAAAAGTC 
TTCCCAGATC CACCCCTGCC GAAACGTCCT CCACCTGTAG AAAAGGAAGA AGACGGCACC 
CTGACTATTG AGCAGCTCGA CAATCTTCGA GATCAGTTCT TAGATATGGC GCCTAAAGGC 
ATAATAGGAA GTAAAGCATT TACTGACATT CTGCTTGATT TGATGACCTT GAACCTTGGA 
ACAAACAACT TCCCTAGTAG CTGGATGCAC CTCACCCAGC CTGAATTACA GGAGTTGACA 
TACTTGTTGA TAGTAAACTC AGAGTTTGTG GACTGGAGGA AATTCCTGTT AGTAACCGCA 
TTGCCTTGGC CCATCCCTCT GGAAGAAGAG CTCCTGGAGA CCCTGCAGAG GTTCAAGGCT 
GTGGATGAGG AGCAGATGGG CACCGTCACT TTTGAGCAGT ATATGCAGGC TGGCCTGTGG 
TTTACAGGAG ATGAAGATAT AAAAATTCCA GAAAATCCTC TTGAACCCCT GCCATATAAT 
AGGCAGGTTC ATCTAATAGA GTTTTTCTTT AGGTTATTTG CTGACTATGA GATGGATCCA 
CCCCAGCTTG ACTACACACA AATGCTGCTG TATTTTGCTT GCCACCCAGA CCCTGTGGAA 
GGGGTCTACA GGGCCCTCAG TGTGGCCATT GGAACTCATA TCTTCCGGAA AGTTGAAGGT 
CCAGTTATGA CAGCAGAAAA GACCTCCTCT ACTGATTTGA GTCCCGTCGA GGGATATGTG 
GAAACTGAGG AAAATGCTGA AAGAGAGAAA AGGGAGTTCA CAGAGGAAAA GGAGGAAAGG 
GATCAGAAGG AAGTGGATAT CCTGGAAAAT GCAAACACTG AAAAGATTTC CATGGAAACA 
CTACTCAGAG TGTTCCGAGG AGGAAGCGAA GTGAAGGATG CGAACAGATT TACCAGCCAC 
CTTAAGAGAG AGAACATTTA TGCAGAGAGC TTCATGAAAG TATTTCAAGA ACTAGAGACC 
AAGGACCTGG AGCCAATTGA AGTTGCTGTT CTCTTGAAGC ATCCTTTCAT CCAAGACCTG 
ATTGCAAATT ATCCAGACTA TAAAATCCCT GATATTAAAA TGATTCTCCA AAGGAGTGTA 
CATGTAGCTG GAAGTGATGG AGAGAGATCA CCCTCAAGAC TTACAGAGGA AAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008260338.1
CDS	224..5323
Translation	MSEILCQWLNQELKVSRTVSPKSFAKAFSTGYLIGEVLHKFELQGDFSEFSESRTSTAKLNNFSRLEPTLHLLGVQFDQNVAQSIITEQPGAATKLLYQLYIALQRKKKSGLTGLEMQTMQPLTNLRLQNMRSEAFREQRLSRRRQNEIMAQIQAAIIQIPKPASNHTLKALEAQKMMKKKKGAEDVANEIKKFEALIKKDLQAKESASKTSVDTTGPMTTDLLNTYSDDDYIKKIQKRLEEDAFAREQREKRRRRLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKDVLWQNRIFREKQYEERRLKDFQDALDREAALAKQAKIDFEEQILREKEIHEQIAVERAQARYKKHYAVCAEILDQILDLSTKVADYRMLTNNLIPHKMMHDWKELFFNGKPIYEQASIKQIAAKPTEEQLIELEKRDLLDSNDYDDYKNMVGEWALPEEMAENLPPSNNCILGHVLHRLVEKSLPPQVESTEPELLSSSVKGCVLGKTFSGKTTTLKSLQKDFPVKILSIDTLVQEAIQAYHDNEKVSEASSIPQEDRKEDQLTVRAQLGAKSEQLLKKGKSIPDMLLVNIMVNAINGIPVNQNWILDGFPMTLNQAQLLEETLTGCNRYLMEAEGKAAQISTLAVDPTTSKEVPLPSSAFDFVILLDISDNSSLSRMNDVMAEEFSRVISHEDTSEHAAAESQHVDVDPNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIRINAEIDKNSLCQKVKEIFATEIMKKKNKVEKRLEEKEAEKKAASLAEPPPPTPPLPPEPEKEKETHQQRDPSKTPPAKGKPQSETSPGKQDSLQDGKGKKGSPKGKPPGGKVQVKKSPVGSTDIPPTPVAPPPPKPGSEEWVYVNEPIPEEIPSFLVPYWELIENSYINTIKTILRHLREDQHEVLKYLYEITTSFQEFLRRPDHKQDFVAQWQADFNSFPDDLWEDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDSLGITMNHFFSLMQAELNRFQDTKKLLQDYYRAMERKIPIDDSKRFTRIPLIRLDSKDISENQLRIPLVPRISISPETGVPRPKLKAILKGKIDYSLENVQTNFEADERLVMDTWQQASLAISNMVAAEIHQRLIEEDKENQPAESKEKSPQMSTNKKGKKENEPPKNKKEDKHGKGKSPPVAEAAPVVFTAEEIAEIEKKNELRLRIKEEHLAALQFEEIATQFRLELIKSKALSVLEDLITKVVDIYKLMEKWLGERYLNEMSSVEKLTEVARYHIETATKIQNELYLNQEDFFINGDIKVFPDPPLPKRPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGIIGSKAFTDILLDLMTLNLGTNNFPSSWMHLTQPELQELTYLLIVNSEFVDWRKFLLVTALPWPIPLEEELLETLQRFKAVDEEQMGTVTFEQYMQAGLWFTGDEDIKIPENPLEPLPYNRQVHLIEFFFRLFADYEMDPPQLDYTQMLLYFACHPDPVEGVYRALSVAIGTHIFRKVEGPVMTAEKTSSTDLSPVEGYVETEENAEREKREFTEEKEERDQKEVDILENANTEKISMETLLRVFRGGSEVKDANRFTSHLKRENIYAESFMKVFQELETKDLEPIEVAVLLKHPFIQDLIANYPDYKIPDIKMILQRSVHVAGSDGERSPSRLTEEKK

Target ORF information:

RefSeq Version	XM_008262116.2
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008262116.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCCCG GACCGTGAGC 
CCCAAGTCAT TTGCAAAGGC ATTTTCCACT GGCTATCTAA TTGGAGAAGT TCTGCACAAG 
TTTGAACTCC AGGGTGACTT CTCAGAATTT TCAGAAAGCA GGACTTCAAC TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTG CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCAAA GCATCATCAC AGAACAGCCT GGAGCGGCCA CAAAACTTTT ATATCAATTG 
TACATTGCTC TTCAGAGAAA AAAGAAAAGT GGCCTCACTG GACTAGAAAT GCAAACCATG 
CAGCCCCTGA CAAATTTAAG ACTTCAGAAC ATGAGAAGTG AGGCTTTTCG AGAGCAACGC 
TTAAGCAGAA GACGACAAAA TGAAATAATG GCCCAAATCC AAGCAGCTAT CATACAGATT 
CCTAAACCAG CATCAAATCA CACTTTGAAA GCACTAGAGG CCCAGAAAAT GATGAAAAAG 
AAAAAAGGTG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG 
GATCTTCAGG CAAAAGAAAG TGCCTCCAAG ACTTCTGTAG ACACAACAGG TCCGATGACT 
ACCGACTTGT TGAACACTTA CTCGGATGAT GACTACATTA AAAAAATCCA GAAGCGCCTA 
GAAGAAGATG CATTTGCACG AGAACAAAGA GAGAAAAGGC GGCGGAGACT GCTAATGGAC 
CAGTTAATAG CGCATGAAGC GCAAGAGGAG GCTTACCGGG AGGAGCAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCGCAGTGC AGCTCATGCA CGTCCGACAT 
GAGAAGGATG TCCTATGGCA AAACAGGATC TTCAGAGAAA AGCAGTATGA GGAAAGGCGA 
CTTAAAGATT TCCAGGATGC ACTTGATCGA GAAGCGGCCT TGGCAAAACA AGCCAAGATT 
GATTTCGAAG AACAAATCCT TAGAGAGAAG GAAATTCATG AGCAGATCGC TGTGGAAAGA 
GCTCAAGCTC GTTACAAAAA GCATTATGCA GTGTGTGCAG AAATCTTGGA TCAGATACTT 
GACTTGTCCA CGAAAGTGGC AGATTATCGT ATGTTGACAA ATAATCTGAT TCCACATAAG 
ATGATGCATG ATTGGAAGGA GCTATTTTTT AATGGAAAAC CCATATATGA GCAAGCCTCC 
ATTAAGCAGA TAGCTGCTAA ACCCACTGAA GAACAACTTA TAGAACTGGA AAAAAGGGAT 
TTGCTAGATA GCAATGACTA CGACGACTAC AAGAATATGG TTGGGGAGTG GGCCTTACCA 
GAAGAAATGG CTGAAAATTT ACCACCTTCC AACAATTGCA TATTAGGCCA TGTGCTTCAC 
AGACTAGTTG AAAAATCTCT TCCTCCTCAA GTTGAATCAA CAGAACCTGA ATTACTTTCA 
TCTTCTGTTA AGGGATGCGT ATTGGGAAAA ACATTTAGTG GGAAAACTAC CACTCTAAAG 
TCTCTACAAA AAGACTTCCC TGTTAAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT ATCATGACAA CGAAAAAGTC AGTGAGGCCT CATCCATTCC TCAAGAGGAT 
AGGAAAGAAG ATCAGCTCAC TGTGCGGGCT CAGCTTGGTG CAAAATCAGA ACAATTGCTG 
AAGAAAGGAA AGAGCATTCC AGACATGTTG CTGGTTAACA TCATGGTAAA CGCTATTAAT 
GGGATACCTG TGAATCAGAA CTGGATCCTT GATGGTTTTC CAATGACATT AAACCAAGCT 
CAACTTCTGG AGGAAACTCT CACAGGCTGC AACAGATACC TCATGGAAGC AGAAGGAAAA 
GCAGCACAAA TATCTACACT GGCTGTGGAT CCTACAACTT CCAAAGAAGT ACCTCTGCCC 
TCTTCTGCAT TTGATTTTGT TATACTATTA GATATTTCTG ATAATTCCTC ACTGAGTCGC 
ATGAATGATG TCATGGCGGA AGAATTTTCA CGTGTAATTT CTCATGAAGA TACCAGTGAG 
CATGCAGCTG CTGAGAGCCA GCATGTAGAT GTGGATCCTA ATTTGAGAGA CCAGATACAA 
CACAGGATTA TAGGCTTCTT GGACAACTGG CCTTTATTGG AGCAATGGTT TTCAGAGCCA 
GAAAATATTT TGATAAGAAT CAATGCTGAA ATAGATAAAA ACTCTTTATG TCAAAAAGTA 
AAAGAAATAT TTGCAACTGA AATAATGAAA AAAAAGAATA AAGTTGAGAA AAGGTTAGAA 
GAAAAGGAAG CTGAGAAAAA AGCAGCTTCG CTGGCTGAGC CTCCACCCCC TACTCCTCCT 
CTTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACTCATCAGC AGCGGGACCC TTCAAAAACG 
CCCCCTGCAA AAGGAAAACC ACAATCAGAA ACTTCACCTG GTAAGCAAGA CTCTCTTCAG 
GATGGAAAAG GAAAGAAAGG TTCTCCTAAA GGAAAACCCC CAGGAGGAAA AGTCCAAGTG 
AAGAAATCAC CTGTTGGCTC TACAGATATA CCACCTACTC CAGTAGCACC ACCGCCCCCT 
AAGCCAGGAT CTGAAGAATG GGTCTATGTG AATGAACCAA TTCCTGAGGA AATACCTTCA 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCTTACA TAAACACTAT CAAAACCATC 
CTCAGGCATC TGAGAGAAGA CCAGCATGAA GTACTTAAAT ACTTATATGA AATTACAACA 
AGTTTCCAGG AGTTTCTAAG GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCTTTTCC TGATGACTTG TGGGAAGATG AGGAAACCAA GGCTGAACTA 
CACCAAAGAG TGAACGATTT ACGAGACCGC CTGTGGGACA TTTGTGATGC CAGGAAGGAA 
GAGGCTGAGC AGGAACGGCT GGATATCATT AACGAAAGCT GGCTGCAGGA CTCCCTTGGA 
ATCACGATGA ACCACTTCTT CTCACTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACA 
AAGAAACTCC TGCAAGATTA TTATAGGGCA ATGGAACGCA AAATCCCAAT AGATGACAGC 
AAGAGATTTA CTCGAATCCC GTTGATCCGA CTGGATAGTA AAGATATTTC AGAAAACCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCCAG AAACAGGCGT GCCCAGACCA 
AAATTAAAAG CAATACTGAA AGGCAAGATT GATTACTCAC TAGAAAACGT GCAGACAAAC 
TTTGAGGCAG ATGAGAGGTT GGTAATGGAC ACCTGGCAGC AGGCTTCCTT AGCGATATCT 
AACATGGTGG CTGCTGAAAT TCATCAGAGG CTCATAGAAG AAGACAAAGA AAACCAACCA 
GCAGAGTCAA AAGAGAAATC TCCCCAGATG AGTACAAACA AAAAAGGCAA AAAGGAAAAT 
GAGCCACCCA AGAATAAAAA GGAAGACAAA CATGGAAAAG GTAAGTCACC ACCTGTAGCT 
GAAGCTGCTC CTGTAGTGTT CACAGCAGAG GAAATCGCAG AAATTGAAAA GAAAAATGAA 
CTGAGGCTCA GAATAAAGGA GGAGCATCTT GCCGCCCTAC AGTTTGAAGA GATAGCTACA 
CAGTTTCGAC TTGAATTGAT AAAATCCAAA GCATTGTCTG TCCTTGAAGA CCTAATAACG 
AAGGTGGTCG ATATATATAA ACTCATGGAA AAATGGCTTG GTGAGAGATA TTTGAATGAA 
ATGTCCAGTG TAGAAAAATT AACAGAAGTA GCTCGCTATC ACATTGAAAC AGCTACAAAA 
ATCCAGAATG AACTTTACTT AAACCAAGAA GACTTCTTCA TTAATGGTGA TATAAAAGTC 
TTCCCAGATC CACCCCTGCC GAAACGTCCT CCACCTGTAG AAAAGGAAGA AGACGGCACC 
CTGACTATTG AGCAGCTCGA CAATCTTCGA GATCAGTTCT TAGATATGGC GCCTAAAGGC 
ATAATAGGAA GTAAAGCATT TACTGACATT CTGCTTGATT TGATGACCTT GAACCTTGGA 
ACAAACAACT TCCCTAGTAG CTGGATGCAC CTCACCCAGC CTGAATTACA GGAGTTGACA 
TACTTGTTGA TAGTAAACTC AGAGTTTGTG GACTGGAGGA AATTCCTGTT AGTAACCGCA 
TTGCCTTGGC CCATCCCTCT GGAAGAAGAG CTCCTGGAGA CCCTGCAGAG GTTCAAGGCT 
GTGGATGAGG AGCAGATGGG CACCGTCACT TTTGAGCAGT ATATGCAGGC TGGCCTGTGG 
TTTACAGGAG ATGAAGATAT AAAAATTCCA GAAAATCCTC TTGAACCCCT GCCATATAAT 
AGGCAGGTTC ATCTAATAGA GTTTTTCTTT AGGTTATTTG CTGACTATGA GATGGATCCA 
CCCCAGCTTG ACTACACACA AATGCTGCTG TATTTTGCTT GCCACCCAGA CCCTGTGGAA 
GGGGTCTACA GGGCCCTCAG TGTGGCCATT GGAACTCATA TCTTCCGGAA AGTTGAAGGT 
CCAGTTATGA CAGCAGAAAA GACCTCCTCT ACTGATTTGA GTCCCGTCGA GGGATATGTG 
GAAACTGAGG AAAATGCTGA AAGAGAGAAA AGGGAGTTCA CAGAGGAAAA GGAGGAAAGG 
GATCAGAAGG AAGTGGATAT CCTGGAAAAT GCAAACACTG AAAAGATTTC CATGGAAACA 
CTACTCAGAG TGTTCCGAGG AGGAAGCGAA GTGAAGGATG CGAACAGATT TACCAGCCAC 
CTTAAGAGAG AGAACATTTA TGCAGAGAGC TTCATGAAAG TATTTCAAGA ACTAGAGACC 
AAGGACCTGG AGCCAATTGA AGTTGCTGTT CTCTTGAAGC ATCCTTTCAT CCAAGACCTG 
ATTGCAAATT ATCCAGACTA TAAAATCCCT GATATTAAAA TGATTCTCCA AAGGAGTGTA 
CATGTAGCTG GAAGTGATGG AGAGAGATCA CCCTCAAGAC TTACAGAGGA AAAGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_008262111.2 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_008260333.1 Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

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CloneID	OOb11239
Clone ID Related Accession (Same CDS sequence)	XM_008262111.2
Accession Version	XM_008262111.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	sperm flagellar protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013679.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2016 this sequence version replaced XM_008262111.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCCCG GACCGTGAGC 
CCCAAGTCAT TTGCAAAGGC ATTTTCCACT GGCTATCTAA TTGGAGAAGT TCTGCACAAG 
TTTGAACTCC AGGGTGACTT CTCAGAATTT TCAGAAAGCA GGACTTCAAC TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTG CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCAAA GCATCATCAC AGAACAGCCT GGAGCGGCCA CAAAACTTTT ATATCAATTG 
TACATTGCTC TTCAGAGAAA AAAGAAAAGT GGCCTCACTG GACTAGAAAT GCAAACCATG 
CAGCCCCTGA CAAATTTAAG ACTTCAGAAC ATGAGAAGTG AGGCTTTTCG AGAGAGACTT 
AAAATCCTGA TACCACGTCA AACTGATTTC AATCTGATGC GGGTTACATA CAGGTTCCAA 
GAAAAATATA GACGCATGAG AGAAGATGTT GACCGTGTGC ATTCTGAGAA ACTTGAAAGA 
ATGCAAAAAC TCAGCGAAGA GCAAAGATGT TTTAATATCG AAAAGCAACG CTTAAGCAGA 
AGACGACAAA ATGAAATAAT GGCCCAAATC CAAGCAGCTA TCATACAGAT TCCTAAACCA 
GCATCAAATC ACACTTTGAA AGCACTAGAG GCCCAGAAAA TGATGAAAAA GAAAAAAGGT 
GCAGAGGATG TGGCCAATGA GATTAAGAAG TTTGAAGCTT TAATAAAAAA GGATCTTCAG 
GCAAAAGAAA GTGCCTCCAA GACTTCTGTA GACACAACAG GTCCGATGAC TACCGACTTG 
TTGAACACTT ACTCGGATGA TGACTACATT AAAAAAATCC AGAAGCGCCT AGAAGAAGAT 
GCATTTGCAC GAGAACAAAG AGAGAAAAGG CGGCGGAGAC TGCTAATGGA CCAGTTAATA 
GCGCATGAAG CGCAAGAGGA GGCTTACCGG GAGGAGCAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATCGCAGTG CAGCTCATGC ACGTCCGACA TGAGAAGGAT 
GTCCTATGGC AAAACAGGAT CTTCAGAGAA AAGCAGTATG AGGAAAGGCG ACTTAAAGAT 
TTCCAGGATG CACTTGATCG AGAAGCGGCC TTGGCAAAAC AAGCCAAGAT TGATTTCGAA 
GAACAAATCC TTAGAGAGAA GGAAATTCAT GAGCAGATCG CTGTGGAAAG AGCTCAAGCT 
CGTTACAAAA AGCATTATGC AGTGTGTGCA GAAATCTTGG ATCAGATACT TGACTTGTCC 
ACGAAAGTGG CAGATTATCG TATGTTGACA AATAATCTGA TTCCACATAA GATGATGCAT 
GATTGGAAGG AGCTATTTTT TAATGGAAAA CCCATATATG AGCAAGCCTC CATTAAGCAG 
ATAGCTGCTA AACCCACTGA AGAACAACTT ATAGAACTGG AAAAAAGGGA TTTGCTAGAT 
AGCAATGACT ACGACGACTA CAAGAATATG GTTGGGGAGT GGGCCTTACC AGAAGAAATG 
GCTGAAAATT TACCACCTTC CAACAATTGC ATATTAGGCC ATGTGCTTCA CAGACTAGTT 
GAAAAATCTC TTCCTCCTCA AGTTGAATCA ACAGAACCTG AATTACTTTC ATCTTCTGTT 
AAGGGATGCG TATTGGGAAA AACATTTAGT GGGAAAACTA CCACTCTAAA GTCTCTACAA 
AAAGACTTCC CTGTTAAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TATCATGACA ACGAAAAAGT CAGTGAGGCC TCATCCATTC CTCAAGAGGA TAGGAAAGAA 
GATCAGCTCA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AACAATTGCT GAAGAAAGGA 
AAGAGCATTC CAGACATGTT GCTGGTTAAC ATCATGGTAA ACGCTATTAA TGGGATACCT 
GTGAATCAGA ACTGGATCCT TGATGGTTTT CCAATGACAT TAAACCAAGC TCAACTTCTG 
GAGGAAACTC TCACAGGCTG CAACAGATAC CTCATGGAAG CAGAAGGAAA AGCAGCACAA 
ATATCTACAC TGGCTGTGGA TCCTACAACT TCCAAAGAAG TACCTCTGCC CTCTTCTGCA 
TTTGATTTTG TTATACTATT AGATATTTCT GATAATTCCT CACTGAGTCG CATGAATGAT 
GTCATGGCGG AAGAATTTTC ACGTGTAATT TCTCATGAAG ATACCAGTGA GCATGCAGCT 
GCTGAGAGCC AGCATGTAGA TGTGGATCCT AATTTGAGAG ACCAGATACA ACACAGGATT 
ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT 
TTGATAAGAA TCAATGCTGA AATAGATAAA AACTCTTTAT GTCAAAAAGT AAAAGAAATA 
TTTGCAACTG AAATAATGAA AAAAAAGAAT AAAGTTGAGA AAAGGTTAGA AGAAAAGGAA 
GCTGAGAAAA AAGCAGCTTC GCTGGCTGAG CCTCCACCCC CTACTCCTCC TCTTCCTCCT 
GAGCCAGAAA AAGAGAAGGA AACTCATCAG CAGCGGGACC CTTCAAAAAC GCCCCCTGCA 
AAAGGAAAAC CACAATCAGA AACTTCACCT GGTAAGCAAG ACTCTCTTCA GGATGGAAAA 
GGAAAGAAAG GTTCTCCTAA AGGAAAACCC CCAGGAGGAA AAGTCCAAGT GAAGAAATCA 
CCTGTTGGCT CTACAGATAT ACCACCTACT CCAGTAGCAC CACCGCCCCC TAAGCCAGGA 
TCTGAAGAAT GGGTCTATGT GAATGAACCA ATTCCTGAGG AAATACCTTC ATTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCTTAC ATAAACACTA TCAAAACCAT CCTCAGGCAT 
CTGAGAGAAG ACCAGCATGA AGTACTTAAA TACTTATATG AAATTACAAC AAGTTTCCAG 
GAGTTTCTAA GGCGTCCAGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCTTTTC CTGATGACTT GTGGGAAGAT GAGGAAACCA AGGCTGAACT ACACCAAAGA 
GTGAACGATT TACGAGACCG CCTGTGGGAC ATTTGTGATG CCAGGAAGGA AGAGGCTGAG 
CAGGAACGGC TGGATATCAT TAACGAAAGC TGGCTGCAGG ACTCCCTTGG AATCACGATG 
AACCACTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC AAAGAAACTC 
CTGCAAGATT ATTATAGGGC AATGGAACGC AAAATCCCAA TAGATGACAG CAAGAGATTT 
ACTCGAATCC CGTTGATCCG ACTGGATAGT AAAGATATTT CAGAAAACCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCCA GAAACAGGCG TGCCCAGACC AAAATTAAAA 
GCAATACTGA AAGGCAAGAT TGATTACTCA CTAGAAAACG TGCAGACAAA CTTTGAGGCA 
GATGAGAGGT TGGTAATGGA CACCTGGCAG CAGGCTTCCT TAGCGATATC TAACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTCATAGAA GAAGACAAAG AAAACCAACC AGCAGAGTCA 
AAAGAGAAAT CTCCCCAGAT GAGTACAAAC AAAAAAGGCA AAAAGGAAAA TGAGCCACCC 
AAGAATAAAA AGGAAGACAA ACATGGAAAA GGTAAGTCAC CACCTGTAGC TGAAGCTGCT 
CCTGTAGTGT TCACAGCAGA GGAAATCGCA GAAATTGAAA AGAAAAATGA ACTGAGGCTC 
AGAATAAAGG AGGAGCATCT TGCCGCCCTA CAGTTTGAAG AGATAGCTAC ACAGTTTCGA 
CTTGAATTGA TAAAATCCAA AGCATTGTCT GTCCTTGAAG ACCTAATAAC GAAGGTGGTC 
GATATATATA AACTCATGGA AAAATGGCTT GGTGAGAGAT ATTTGAATGA AATGTCCAGT 
GTAGAAAAAT TAACAGAAGT AGCTCGCTAT CACATTGAAA CAGCTACAAA AATCCAGAAT 
GAACTTTACT TAAACCAAGA AGACTTCTTC ATTAATGGTG ATATAAAAGT CTTCCCAGAT 
CCACCCCTGC CGAAACGTCC TCCACCTGTA GAAAAGGAAG AAGACGGCAC CCTGACTATT 
GAGCAGCTCG ACAATCTTCG AGATCAGTTC TTAGATATGG CGCCTAAAGG CAAGGATCCT 
TCGGATGCTG TGAGTCAAGT CACCAAACAG CAGAAAAGGC CACAGAAAAT CAGCCACTAC 
TTTCCAATGA GATACAATCA GTTTTTGAAA ATTTGGTTAA AAAAATACCC TTGGCTTGTC 
TATGATGACT TGTTAAATCT TATGTTCTGT GCCCTGTGTC GTAAGCATGG AGTGAAGTCA 
GGCGGAAGTC AAATGAGTTT TTTTTATGGC ACAGACAACT TTAGGACTGA ATTTCTCAAT 
GCACATCATC TCAGTGAGGC TCATGCCAAG GCTTTATTAA TGGAAGCAAC AAGTGGTTCT 
TCAGAGAACA GAGCCACCAC AGAACTAATG ATGAGGACCA TGAGCAAAGT AACTCTTGGG 
AGAGTAGAGA ATTTATTCAG ATCATGCCAT GCTATTGCCA AGACTGGACG TCCCCTCAAA 
GATTTTATCT GGATGTGCAA GTTGGATGAC ATGAAGGGTG TTGATATTGG CCCAGTATTC 
AGAACTAACA AGTCAGCACA AATCTTTACA TATTTCATTG CTGAAGTAGA GCGGAAAAGT 
CTAAGAGAGA AGCTCGAAAA AAGTAAATTT TTCTCTGTCA TCAGTGATGG AATCACAGAC 
AGTTTAATCA AGGAAGTCAA ACTTGTTTAT GTACAGTTTG CACATGCAGG AAAAGTGCAT 
TGTCAAATTG TTGGGGCACA GACAATAGAA AGGAAAGATC CCCTGGCAAT TAAAAATGCC 
ATCGAGAAAA CTCTAGAGAT AAATCTGCAA CTTAGGCTAT CAAGCCAAGA TTGGGCAACG 
AAACTGGTTG GTTTTGGAAG TGATGGTCCT CCTGACATAG AAGGAGAGAA AACTGAGGTG 
ACCTTGCTTT TAAGAGAAAT CCAGCCATGT GTGCAGACTG TTTATTGCTT TGCCCATCAC 
CTTGAACTAT CCTACAAGGC AGTGTTCCAG AGCATTCCTC TGTACGACAG TGTCAGAGAG 
CTTCTTCGCA GCATTTATCA CTTCTATCAC ACTTCTCCTC TTCGAAAGAG TTCTCTGATA 
GCTGCCTTCA AAGACCTTCA CCTTCGACCC ACAATACCTT CTCGAATAGA AGGTAGGAGG 
TGGCTTAATG GATTACAGAC AGCCCTCCAG AACTTTCTCA AGGGATATCC TGCAATTATC 
CACCAATTGC ATTCTGAAGG TGAAGAAAGA AGTGACACCA GCCAACAGAA AGCCAAAGAC 
CTGCTTGAGT TGCTGCTCCA AGACAGCATT GTCAAGTTTG CCCATTTCTT AGTGGATGTC 
GTTAACGTCC TCAGCATTCT GTCCCGCGTC AGTCAGAGCA GAAATTCCTC AATTGCTGAC 
ATATCCGCCA CCATGGAGTC CACACTGAAA ATGCTCCAAA TGTATCAGAC AAGGCCAGGC 
CCAAAAGAAT GCAAGGTGGA TGCAGTCACA CACTTTCACG GTAGCCGCCT CAGGGGAACA 
GAAAACATCT CTGCTGTGAG AAACGTGGTT TTAACACATC TTATCAAGAG GCTTCAAGGC 
TGCTTCAGAG ATGCCAGCCA AGATGTGGTG AGGGCAACCA TGATCGTAAG CTTTAAATTG 
TGGCCCACAA GGATAAATCA AGAATTTGGA GAGAAAGAGG TCTCCATCCT GACTTCACAT 
TACGAACCAG TATTAGAAGC CGCCAGCGTG AAAATTGATG AAGTGGACAC CGAGTGGAGC 
ATGTTGAAAT TAGAGATTTA TGCCAGATTT CAGAACATTC AAAAACTGAC CTGGGATTTT 
GTGAATTCCA TTTACTTACA CAAGTATCCA AATATTCTGA GGCTTGTTGA CCTAGTGCTG 
ACCCTCCCAG CAAGTTCAGC TGAAGCAGAA CGTGGCTTTA GTCAGATGAA ACGCATCACG 
TCACACACGC ATGCTAAAGT CACATCTGAA AGCATCACGG ATACCCTGAT CATTCAGCTG 
AACTCTCCAG ACATTAATAA TTTTGACCCT AGGAAAGCTA TCCATTTGTG GAATACAAGA 
ATGCTGTCTT CAGCTGGTGA CACAGGACCT AATTCAGATT GCTCCTCAGA CTCAGAAAGC 
CCTTGTGAAA GTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008260333.1
CDS	229..6546
Translation	MSEILCQWLNQELKVSRTVSPKSFAKAFSTGYLIGEVLHKFELQGDFSEFSESRTSTAKLNNFSRLEPTLHLLGVQFDQNVAQSIITEQPGAATKLLYQLYIALQRKKKSGLTGLEMQTMQPLTNLRLQNMRSEAFRERLKILIPRQTDFNLMRVTYRFQEKYRRMREDVDRVHSEKLERMQKLSEEQRCFNIEKQRLSRRRQNEIMAQIQAAIIQIPKPASNHTLKALEAQKMMKKKKGAEDVANEIKKFEALIKKDLQAKESASKTSVDTTGPMTTDLLNTYSDDDYIKKIQKRLEEDAFAREQREKRRRRLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKDVLWQNRIFREKQYEERRLKDFQDALDREAALAKQAKIDFEEQILREKEIHEQIAVERAQARYKKHYAVCAEILDQILDLSTKVADYRMLTNNLIPHKMMHDWKELFFNGKPIYEQASIKQIAAKPTEEQLIELEKRDLLDSNDYDDYKNMVGEWALPEEMAENLPPSNNCILGHVLHRLVEKSLPPQVESTEPELLSSSVKGCVLGKTFSGKTTTLKSLQKDFPVKILSIDTLVQEAIQAYHDNEKVSEASSIPQEDRKEDQLTVRAQLGAKSEQLLKKGKSIPDMLLVNIMVNAINGIPVNQNWILDGFPMTLNQAQLLEETLTGCNRYLMEAEGKAAQISTLAVDPTTSKEVPLPSSAFDFVILLDISDNSSLSRMNDVMAEEFSRVISHEDTSEHAAAESQHVDVDPNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIRINAEIDKNSLCQKVKEIFATEIMKKKNKVEKRLEEKEAEKKAASLAEPPPPTPPLPPEPEKEKETHQQRDPSKTPPAKGKPQSETSPGKQDSLQDGKGKKGSPKGKPPGGKVQVKKSPVGSTDIPPTPVAPPPPKPGSEEWVYVNEPIPEEIPSFLVPYWELIENSYINTIKTILRHLREDQHEVLKYLYEITTSFQEFLRRPDHKQDFVAQWQADFNSFPDDLWEDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDSLGITMNHFFSLMQAELNRFQDTKKLLQDYYRAMERKIPIDDSKRFTRIPLIRLDSKDISENQLRIPLVPRISISPETGVPRPKLKAILKGKIDYSLENVQTNFEADERLVMDTWQQASLAISNMVAAEIHQRLIEEDKENQPAESKEKSPQMSTNKKGKKENEPPKNKKEDKHGKGKSPPVAEAAPVVFTAEEIAEIEKKNELRLRIKEEHLAALQFEEIATQFRLELIKSKALSVLEDLITKVVDIYKLMEKWLGERYLNEMSSVEKLTEVARYHIETATKIQNELYLNQEDFFINGDIKVFPDPPLPKRPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGKDPSDAVSQVTKQQKRPQKISHYFPMRYNQFLKIWLKKYPWLVYDDLLNLMFCALCRKHGVKSGGSQMSFFYGTDNFRTEFLNAHHLSEAHAKALLMEATSGSSENRATTELMMRTMSKVTLGRVENLFRSCHAIAKTGRPLKDFIWMCKLDDMKGVDIGPVFRTNKSAQIFTYFIAEVERKSLREKLEKSKFFSVISDGITDSLIKEVKLVYVQFAHAGKVHCQIVGAQTIERKDPLAIKNAIEKTLEINLQLRLSSQDWATKLVGFGSDGPPDIEGEKTEVTLLLREIQPCVQTVYCFAHHLELSYKAVFQSIPLYDSVRELLRSIYHFYHTSPLRKSSLIAAFKDLHLRPTIPSRIEGRRWLNGLQTALQNFLKGYPAIIHQLHSEGEERSDTSQQKAKDLLELLLQDSIVKFAHFLVDVVNVLSILSRVSQSRNSSIADISATMESTLKMLQMYQTRPGPKECKVDAVTHFHGSRLRGTENISAVRNVVLTHLIKRLQGCFRDASQDVVRATMIVSFKLWPTRINQEFGEKEVSILTSHYEPVLEAASVKIDEVDTEWSMLKLEIYARFQNIQKLTWDFVNSIYLHKYPNILRLVDLVLTLPASSAEAERGFSQMKRITSHTHAKVTSESITDTLIIQLNSPDINNFDPRKAIHLWNTRMLSSAGDTGPNSDCSSDSESPCESD

Target ORF information:

RefSeq Version	XM_008262111.2
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008262111.2

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCCCG GACCGTGAGC 
CCCAAGTCAT TTGCAAAGGC ATTTTCCACT GGCTATCTAA TTGGAGAAGT TCTGCACAAG 
TTTGAACTCC AGGGTGACTT CTCAGAATTT TCAGAAAGCA GGACTTCAAC TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTG CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCAAA GCATCATCAC AGAACAGCCT GGAGCGGCCA CAAAACTTTT ATATCAATTG 
TACATTGCTC TTCAGAGAAA AAAGAAAAGT GGCCTCACTG GACTAGAAAT GCAAACCATG 
CAGCCCCTGA CAAATTTAAG ACTTCAGAAC ATGAGAAGTG AGGCTTTTCG AGAGAGACTT 
AAAATCCTGA TACCACGTCA AACTGATTTC AATCTGATGC GGGTTACATA CAGGTTCCAA 
GAAAAATATA GACGCATGAG AGAAGATGTT GACCGTGTGC ATTCTGAGAA ACTTGAAAGA 
ATGCAAAAAC TCAGCGAAGA GCAAAGATGT TTTAATATCG AAAAGCAACG CTTAAGCAGA 
AGACGACAAA ATGAAATAAT GGCCCAAATC CAAGCAGCTA TCATACAGAT TCCTAAACCA 
GCATCAAATC ACACTTTGAA AGCACTAGAG GCCCAGAAAA TGATGAAAAA GAAAAAAGGT 
GCAGAGGATG TGGCCAATGA GATTAAGAAG TTTGAAGCTT TAATAAAAAA GGATCTTCAG 
GCAAAAGAAA GTGCCTCCAA GACTTCTGTA GACACAACAG GTCCGATGAC TACCGACTTG 
TTGAACACTT ACTCGGATGA TGACTACATT AAAAAAATCC AGAAGCGCCT AGAAGAAGAT 
GCATTTGCAC GAGAACAAAG AGAGAAAAGG CGGCGGAGAC TGCTAATGGA CCAGTTAATA 
GCGCATGAAG CGCAAGAGGA GGCTTACCGG GAGGAGCAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATCGCAGTG CAGCTCATGC ACGTCCGACA TGAGAAGGAT 
GTCCTATGGC AAAACAGGAT CTTCAGAGAA AAGCAGTATG AGGAAAGGCG ACTTAAAGAT 
TTCCAGGATG CACTTGATCG AGAAGCGGCC TTGGCAAAAC AAGCCAAGAT TGATTTCGAA 
GAACAAATCC TTAGAGAGAA GGAAATTCAT GAGCAGATCG CTGTGGAAAG AGCTCAAGCT 
CGTTACAAAA AGCATTATGC AGTGTGTGCA GAAATCTTGG ATCAGATACT TGACTTGTCC 
ACGAAAGTGG CAGATTATCG TATGTTGACA AATAATCTGA TTCCACATAA GATGATGCAT 
GATTGGAAGG AGCTATTTTT TAATGGAAAA CCCATATATG AGCAAGCCTC CATTAAGCAG 
ATAGCTGCTA AACCCACTGA AGAACAACTT ATAGAACTGG AAAAAAGGGA TTTGCTAGAT 
AGCAATGACT ACGACGACTA CAAGAATATG GTTGGGGAGT GGGCCTTACC AGAAGAAATG 
GCTGAAAATT TACCACCTTC CAACAATTGC ATATTAGGCC ATGTGCTTCA CAGACTAGTT 
GAAAAATCTC TTCCTCCTCA AGTTGAATCA ACAGAACCTG AATTACTTTC ATCTTCTGTT 
AAGGGATGCG TATTGGGAAA AACATTTAGT GGGAAAACTA CCACTCTAAA GTCTCTACAA 
AAAGACTTCC CTGTTAAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TATCATGACA ACGAAAAAGT CAGTGAGGCC TCATCCATTC CTCAAGAGGA TAGGAAAGAA 
GATCAGCTCA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AACAATTGCT GAAGAAAGGA 
AAGAGCATTC CAGACATGTT GCTGGTTAAC ATCATGGTAA ACGCTATTAA TGGGATACCT 
GTGAATCAGA ACTGGATCCT TGATGGTTTT CCAATGACAT TAAACCAAGC TCAACTTCTG 
GAGGAAACTC TCACAGGCTG CAACAGATAC CTCATGGAAG CAGAAGGAAA AGCAGCACAA 
ATATCTACAC TGGCTGTGGA TCCTACAACT TCCAAAGAAG TACCTCTGCC CTCTTCTGCA 
TTTGATTTTG TTATACTATT AGATATTTCT GATAATTCCT CACTGAGTCG CATGAATGAT 
GTCATGGCGG AAGAATTTTC ACGTGTAATT TCTCATGAAG ATACCAGTGA GCATGCAGCT 
GCTGAGAGCC AGCATGTAGA TGTGGATCCT AATTTGAGAG ACCAGATACA ACACAGGATT 
ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT 
TTGATAAGAA TCAATGCTGA AATAGATAAA AACTCTTTAT GTCAAAAAGT AAAAGAAATA 
TTTGCAACTG AAATAATGAA AAAAAAGAAT AAAGTTGAGA AAAGGTTAGA AGAAAAGGAA 
GCTGAGAAAA AAGCAGCTTC GCTGGCTGAG CCTCCACCCC CTACTCCTCC TCTTCCTCCT 
GAGCCAGAAA AAGAGAAGGA AACTCATCAG CAGCGGGACC CTTCAAAAAC GCCCCCTGCA 
AAAGGAAAAC CACAATCAGA AACTTCACCT GGTAAGCAAG ACTCTCTTCA GGATGGAAAA 
GGAAAGAAAG GTTCTCCTAA AGGAAAACCC CCAGGAGGAA AAGTCCAAGT GAAGAAATCA 
CCTGTTGGCT CTACAGATAT ACCACCTACT CCAGTAGCAC CACCGCCCCC TAAGCCAGGA 
TCTGAAGAAT GGGTCTATGT GAATGAACCA ATTCCTGAGG AAATACCTTC ATTTTTAGTA 
CCTTACTGGG AACTAATAGA AAATTCTTAC ATAAACACTA TCAAAACCAT CCTCAGGCAT 
CTGAGAGAAG ACCAGCATGA AGTACTTAAA TACTTATATG AAATTACAAC AAGTTTCCAG 
GAGTTTCTAA GGCGTCCAGA TCACAAGCAA GATTTTGTAG CTCAATGGCA GGCTGATTTC 
AACTCTTTTC CTGATGACTT GTGGGAAGAT GAGGAAACCA AGGCTGAACT ACACCAAAGA 
GTGAACGATT TACGAGACCG CCTGTGGGAC ATTTGTGATG CCAGGAAGGA AGAGGCTGAG 
CAGGAACGGC TGGATATCAT TAACGAAAGC TGGCTGCAGG ACTCCCTTGG AATCACGATG 
AACCACTTCT TCTCACTGAT GCAGGCAGAA CTGAACCGTT TCCAAGATAC AAAGAAACTC 
CTGCAAGATT ATTATAGGGC AATGGAACGC AAAATCCCAA TAGATGACAG CAAGAGATTT 
ACTCGAATCC CGTTGATCCG ACTGGATAGT AAAGATATTT CAGAAAACCA GCTTAGAATT 
CCTCTAGTTC CTCGAATATC CATTTCTCCA GAAACAGGCG TGCCCAGACC AAAATTAAAA 
GCAATACTGA AAGGCAAGAT TGATTACTCA CTAGAAAACG TGCAGACAAA CTTTGAGGCA 
GATGAGAGGT TGGTAATGGA CACCTGGCAG CAGGCTTCCT TAGCGATATC TAACATGGTG 
GCTGCTGAAA TTCATCAGAG GCTCATAGAA GAAGACAAAG AAAACCAACC AGCAGAGTCA 
AAAGAGAAAT CTCCCCAGAT GAGTACAAAC AAAAAAGGCA AAAAGGAAAA TGAGCCACCC 
AAGAATAAAA AGGAAGACAA ACATGGAAAA GGTAAGTCAC CACCTGTAGC TGAAGCTGCT 
CCTGTAGTGT TCACAGCAGA GGAAATCGCA GAAATTGAAA AGAAAAATGA ACTGAGGCTC 
AGAATAAAGG AGGAGCATCT TGCCGCCCTA CAGTTTGAAG AGATAGCTAC ACAGTTTCGA 
CTTGAATTGA TAAAATCCAA AGCATTGTCT GTCCTTGAAG ACCTAATAAC GAAGGTGGTC 
GATATATATA AACTCATGGA AAAATGGCTT GGTGAGAGAT ATTTGAATGA AATGTCCAGT 
GTAGAAAAAT TAACAGAAGT AGCTCGCTAT CACATTGAAA CAGCTACAAA AATCCAGAAT 
GAACTTTACT TAAACCAAGA AGACTTCTTC ATTAATGGTG ATATAAAAGT CTTCCCAGAT 
CCACCCCTGC CGAAACGTCC TCCACCTGTA GAAAAGGAAG AAGACGGCAC CCTGACTATT 
GAGCAGCTCG ACAATCTTCG AGATCAGTTC TTAGATATGG CGCCTAAAGG CAAGGATCCT 
TCGGATGCTG TGAGTCAAGT CACCAAACAG CAGAAAAGGC CACAGAAAAT CAGCCACTAC 
TTTCCAATGA GATACAATCA GTTTTTGAAA ATTTGGTTAA AAAAATACCC TTGGCTTGTC 
TATGATGACT TGTTAAATCT TATGTTCTGT GCCCTGTGTC GTAAGCATGG AGTGAAGTCA 
GGCGGAAGTC AAATGAGTTT TTTTTATGGC ACAGACAACT TTAGGACTGA ATTTCTCAAT 
GCACATCATC TCAGTGAGGC TCATGCCAAG GCTTTATTAA TGGAAGCAAC AAGTGGTTCT 
TCAGAGAACA GAGCCACCAC AGAACTAATG ATGAGGACCA TGAGCAAAGT AACTCTTGGG 
AGAGTAGAGA ATTTATTCAG ATCATGCCAT GCTATTGCCA AGACTGGACG TCCCCTCAAA 
GATTTTATCT GGATGTGCAA GTTGGATGAC ATGAAGGGTG TTGATATTGG CCCAGTATTC 
AGAACTAACA AGTCAGCACA AATCTTTACA TATTTCATTG CTGAAGTAGA GCGGAAAAGT 
CTAAGAGAGA AGCTCGAAAA AAGTAAATTT TTCTCTGTCA TCAGTGATGG AATCACAGAC 
AGTTTAATCA AGGAAGTCAA ACTTGTTTAT GTACAGTTTG CACATGCAGG AAAAGTGCAT 
TGTCAAATTG TTGGGGCACA GACAATAGAA AGGAAAGATC CCCTGGCAAT TAAAAATGCC 
ATCGAGAAAA CTCTAGAGAT AAATCTGCAA CTTAGGCTAT CAAGCCAAGA TTGGGCAACG 
AAACTGGTTG GTTTTGGAAG TGATGGTCCT CCTGACATAG AAGGAGAGAA AACTGAGGTG 
ACCTTGCTTT TAAGAGAAAT CCAGCCATGT GTGCAGACTG TTTATTGCTT TGCCCATCAC 
CTTGAACTAT CCTACAAGGC AGTGTTCCAG AGCATTCCTC TGTACGACAG TGTCAGAGAG 
CTTCTTCGCA GCATTTATCA CTTCTATCAC ACTTCTCCTC TTCGAAAGAG TTCTCTGATA 
GCTGCCTTCA AAGACCTTCA CCTTCGACCC ACAATACCTT CTCGAATAGA AGGTAGGAGG 
TGGCTTAATG GATTACAGAC AGCCCTCCAG AACTTTCTCA AGGGATATCC TGCAATTATC 
CACCAATTGC ATTCTGAAGG TGAAGAAAGA AGTGACACCA GCCAACAGAA AGCCAAAGAC 
CTGCTTGAGT TGCTGCTCCA AGACAGCATT GTCAAGTTTG CCCATTTCTT AGTGGATGTC 
GTTAACGTCC TCAGCATTCT GTCCCGCGTC AGTCAGAGCA GAAATTCCTC AATTGCTGAC 
ATATCCGCCA CCATGGAGTC CACACTGAAA ATGCTCCAAA TGTATCAGAC AAGGCCAGGC 
CCAAAAGAAT GCAAGGTGGA TGCAGTCACA CACTTTCACG GTAGCCGCCT CAGGGGAACA 
GAAAACATCT CTGCTGTGAG AAACGTGGTT TTAACACATC TTATCAAGAG GCTTCAAGGC 
TGCTTCAGAG ATGCCAGCCA AGATGTGGTG AGGGCAACCA TGATCGTAAG CTTTAAATTG 
TGGCCCACAA GGATAAATCA AGAATTTGGA GAGAAAGAGG TCTCCATCCT GACTTCACAT 
TACGAACCAG TATTAGAAGC CGCCAGCGTG AAAATTGATG AAGTGGACAC CGAGTGGAGC 
ATGTTGAAAT TAGAGATTTA TGCCAGATTT CAGAACATTC AAAAACTGAC CTGGGATTTT 
GTGAATTCCA TTTACTTACA CAAGTATCCA AATATTCTGA GGCTTGTTGA CCTAGTGCTG 
ACCCTCCCAG CAAGTTCAGC TGAAGCAGAA CGTGGCTTTA GTCAGATGAA ACGCATCACG 
TCACACACGC ATGCTAAAGT CACATCTGAA AGCATCACGG ATACCCTGAT CATTCAGCTG 
AACTCTCCAG ACATTAATAA TTTTGACCCT AGGAAAGCTA TCCATTTGTG GAATACAAGA 
ATGCTGTCTT CAGCTGGTGA CACAGGACCT AATTCAGATT GCTCCTCAGA CTCAGAAAGC 
CCTTGTGAAA GTGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_017344675.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_017200164.1 Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

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CloneID	OOb35039
Clone ID Related Accession (Same CDS sequence)	XM_017344675.1
Accession Version	XM_017344675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	sperm flagellar protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013679.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCCCG GACCGTGAGC 
CCCAAGTCAT TTGCAAAGGC ATTTTCCACT GGCTATCTAA TTGGAGAAGT TCTGCACAAG 
TTTGAACTCC AGGGTGACTT CTCAGAATTT TCAGAAAGCA GGACTTCAAC TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTG CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCAAA GCATCATCAC AGAACAGCCT GGAGCGGCCA CAAAACTTTT ATATCAATTG 
TACATTGCTC TTCAGAGAAA AAAGAAAAGT GGCCTCACTG GACTAGAAAT GCAAACCATG 
CAGCCCCTGA CAAATTTAAG ACTTCAGAAC ATGAGAAGTG AGGCTTTTCG AGAGAGACTT 
AAAATCCTGA TACCACGTCA AACTGATTTC AATCTGATGC GGGTTACATA CAGGTTCCAA 
GAAAAATATA GACGCATGAG AGAAGATGTT GACCGTGTGC ATTCTGAGAA ACTTGAAAGA 
ATGCAAAAAC TCAGCGAAGA GCAAAGATGT TTTAATATCG AAAAGCAACG CTTAAGCAGA 
AGACGACAAA ATGAAATAAT GGCCCAAATC CAAGCAGCTA TCATACAGAT TCCTAAACCA 
GCATCAAATC ACACTTTGAA AGCACTAGAG GCCCAGAAAA TGATGAAAAA GAAAAAAGGT 
GCAGAGGATG TGGCCAATGA GATTAAGAAG TTTGAAGCTT TAATAAAAAA GGATCTTCAG 
GCAAAAGAAA GTGCCTCCAA GACTTCTGTA GACACAACAG GTCCGATGAC TACCGACTTG 
TTGAACACTT ACTCGGATGA TGACTACATT AAAAAAATCC AGAAGCGCCT AGAAGAAGAT 
GCATTTGCAC GAGAACAAAG AGAGAAAAGG CGGCGGAGAC TGCTAATGGA CCAGTTAATA 
GCGCATGAAG CGCAAGAGGA GGCTTACCGG GAGGAGCAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATCGCAGTG CAGCTCATGC ACGTCCGACA TGAGAAGGAT 
GTCCTATGGC AAAACAGGAT CTTCAGAGAA AAGCAGTATG AGGAAAGGCG ACTTAAAGAT 
TTCCAGGATG CACTTGATCG AGAAGCGGCC TTGGCAAAAC AAGCCAAGAT TGATTTCGAA 
GAACAAATCC TTAGAGAGAA GGAAATTCAT GAGCAGATCG CTGTGGAAAG AGCTCAAGCT 
CGTTACAAAA AGCATTATGC AGTGTGTGCA GAAATCTTGG ATCAGATACT TGACTTGTCC 
ACGAAAGTGG CAGATTATCG TATGTTGACA AATAATCTGA TTCCACATAA GATGATGCAT 
GATTGGAAGG AGCTATTTTT TAATGGAAAA CCCATATATG AGCAAGCCTC CATTAAGCAG 
ATAGCTGCTA AACCCACTGA AGAACAACTT ATAGAACTGG AAAAAAGGGA TTTGCTAGAT 
AGCAATGACT ACGACGACTA CAAGAATATG GTTGGGGAGT GGGCCTTACC AGAAGAAATG 
GCTGAAAATT TACCACCTTC CAACAATTGC ATATTAGGCC ATGTGCTTCA CAGACTAGTT 
GAAAAATCTC TTCCTCCTCA AGTTGAATCA ACAGAACCTG AATTACTTTC ATCTTCTGTT 
AAGGGATGCG TATTGGGAAA AACATTTAGT GGGAAAACTA CCACTCTAAA GTCTCTACAA 
AAAGACTTCC CTGTTAAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TATCATGACA ACGAAAAAGT CAGTGAGGCC TCATCCATTC CTCAAGAGGA TAGGAAAGAA 
GATCAGCTCA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AACAATTGCT GAAGAAAGGA 
AAGAGCATTC CAGACATGTT GCTGGTTAAC ATCATGGTAA ACGCTATTAA TGGGATACCT 
GTGAATCAGA ACTGGATCCT TGATGGTTTT CCAATGACAT TAAACCAAGC TCAACTTCTG 
GAGGAAACTC TCACAGGCTG CAACAGATAC CTCATGGAAG CAGAAGGAAA AGCAGCACAA 
ATATCTACAC TGGCTGTGGA TCCTACAACT TCCAAAGAAG TACCTCTGCC CTCTTCTGCA 
TTTGATTTTG TTATACTATT AGATATTTCT GATAATTCCT CACTGAGTCG CATGAATGAT 
GTCATGGCGG AAGAATTTTC ACGTGTAATT TCTCATGAAG ATACCAGTGA GCATGCAGCT 
GCTGAGAGCC AGCATGTAGA TGTGGATCCT AATTTGAGAG ACCAGATACA ACACAGGATT 
ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT 
TTGATAAGAA TCAATGCTGA AATAGATAAA AACTCTTTAT GTCAAAAAGT AAAAGAAATA 
TTTGCAACTG AAATAATGAA AAAAAAGAAT AAAGTTGAGA AAAGGTTAGA AGAAAAGGAA 
GCTGAGAAAA AAGCAGCTTC GCTGGCTGAG CCTCCACCCC CTACTCCTCC TCTTCCTCCT 
GAGCCAGAAA AAGAGAAGGA AACTCATCAG CAGCGGGACC CTTCAAAAAC GCCCCCTGCA 
AAAGGAAAAC CACAATCAGG TTCTCCTAAA GGAAAACCCC CAGGAGGAAA AGTCCAAGTG 
AAGAAATCAC CTGTTGGCTC TACAGATATA CCACCTACTC CAGTAGCACC ACCGCCCCCT 
AAGCCAGGAT CTGAAGAATG GGTCTATGTG AATGAACCAA TTCCTGAGGA AATACCTTCA 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCTTACA TAAACACTAT CAAAACCATC 
CTCAGGCATC TGAGAGAAGA CCAGCATGAA GTACTTAAAT ACTTATATGA AATTACAACA 
AGTTTCCAGG AGTTTCTAAG GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCTTTTCC TGATGACTTG TGGGAAGATG AGGAAACCAA GGCTGAACTA 
CACCAAAGAG TGAACGATTT ACGAGACCGC CTGTGGGACA TTTGTGATGC CAGGAAGGAA 
GAGGCTGAGC AGGAACGGCT GGATATCATT AACGAAAGCT GGCTGCAGGA CTCCCTTGGA 
ATCACGATGA ACCACTTCTT CTCACTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACA 
AAGAAACTCC TGCAAGATTA TTATAGGGCA ATGGAACGCA AAATCCCAAT AGATGACAGC 
AAGAGATTTA CTCGAATCCC GTTGATCCGA CTGGATAGTA AAGATATTTC AGAAAACCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCCAG AAACAGGCGT GCCCAGACCA 
AAATTAAAAG CAATACTGAA AGGCAAGATT GATTACTCAC TAGAAAACGT GCAGACAAAC 
TTTGAGGCAG ATGAGAGGTT GGTAATGGAC ACCTGGCAGC AGGCTTCCTT AGCGATATCT 
AACATGGTGG CTGCTGAAAT TCATCAGAGG CTCATAGAAG AAGACAAAGA AAACCAACCA 
GCAGAGTCAA AAGAGAAATC TCCCCAGATG AGTACAAACA AAAAAGGCAA AAAGGAAAAT 
GAGCCACCCA AGAATAAAAA GGAAGACAAA CATGGAAAAG GTAAGTCACC ACCTGTAGCT 
GAAGCTGCTC CTGTAGTGTT CACAGCAGAG GAAATCGCAG AAATTGAAAA GAAAAATGAA 
CTGAGGCTCA GAATAAAGGA GGAGCATCTT GCCGCCCTAC AGTTTGAAGA GATAGCTACA 
CAGTTTCGAC TTGAATTGAT AAAATCCAAA GCATTGTCTG TCCTTGAAGA CCTAATAACG 
AAGGTGGTCG ATATATATAA ACTCATGGAA AAATGGCTTG GTGAGAGATA TTTGAATGAA 
ATGTCCAGTG TAGAAAAATT AACAGAAGTA GCTCGCTATC ACATTGAAAC AGCTACAAAA 
ATCCAGAATG AACTTTACTT AAACCAAGAA GACTTCTTCA TTAATGGTGA TATAAAAGTC 
TTCCCAGATC CACCCCTGCC GAAACGTCCT CCACCTGTAG AAAAGGAAGA AGACGGCACC 
CTGACTATTG AGCAGCTCGA CAATCTTCGA GATCAGTTCT TAGATATGGC GCCTAAAGGC 
AAGGATCCTT CGGATGCTGT GAGTCAAGTC ACCAAACAGC AGAAAAGGCC ACAGAAAATC 
AGCCACTACT TTCCAATGAG ATACAATCAG TTTTTGAAAA TTTGGTTAAA AAAATACCCT 
TGGCTTGTCT ATGATGACTT GTTAAATCTT ATGTTCTGTG CCCTGTGTCG TAAGCATGGA 
GTGAAGTCAG GCGGAAGTCA AATGAGTTTT TTTTATGGCA CAGACAACTT TAGGACTGAA 
TTTCTCAATG CACATCATCT CAGTGAGGCT CATGCCAAGG CTTTATTAAT GGAAGCAACA 
AGTGGTTCTT CAGAGAACAG AGCCACCACA GAACTAATGA TGAGGACCAT GAGCAAAGTA 
ACTCTTGGGA GAGTAGAGAA TTTATTCAGA TCATGCCATG CTATTGCCAA GACTGGACGT 
CCCCTCAAAG ATTTTATCTG GATGTGCAAG TTGGATGACA TGAAGGGTGT TGATATTGGC 
CCAGTATTCA GAACTAACAA GTCAGCACAA ATCTTTACAT ATTTCATTGC TGAAGTAGAG 
CGGAAAAGTC TAAGAGAGAA GCTCGAAAAA AGTAAATTTT TCTCTGTCAT CAGTGATGGA 
ATCACAGACA GTTTAATCAA GGAAGTCAAA CTTGTTTATG TACAGTTTGC ACATGCAGGA 
AAAGTGCATT GTCAAATTGT TGGGGCACAG ACAATAGAAA GGAAAGATCC CCTGGCAATT 
AAAAATGCCA TCGAGAAAAC TCTAGAGATA AATCTGCAAC TTAGGCTATC AAGCCAAGAT 
TGGGCAACGA AACTGGTTGG TTTTGGAAGT GATGGTCCTC CTGACATAGA AGGAGAGAAA 
ACTGAGGTGA CCTTGCTTTT AAGAGAAATC CAGCCATGTG TGCAGACTGT TTATTGCTTT 
GCCCATCACC TTGAACTATC CTACAAGGCA GTGTTCCAGA GCATTCCTCT GTACGACAGT 
GTCAGAGAGC TTCTTCGCAG CATTTATCAC TTCTATCACA CTTCTCCTCT TCGAAAGAGT 
TCTCTGATAG CTGCCTTCAA AGACCTTCAC CTTCGACCCA CAATACCTTC TCGAATAGAA 
GGTAGGAGGT GGCTTAATGG ATTACAGACA GCCCTCCAGA ACTTTCTCAA GGGATATCCT 
GCAATTATCC ACCAATTGCA TTCTGAAGGT GAAGAAAGAA GTGACACCAG CCAACAGAAA 
GCCAAAGACC TGCTTGAGTT GCTGCTCCAA GACAGCATTG TCAAGTTTGC CCATTTCTTA 
GTGGATGTCG TTAACGTCCT CAGCATTCTG TCCCGCGTCA GTCAGAGCAG AAATTCCTCA 
ATTGCTGACA TATCCGCCAC CATGGAGTCC ACACTGAAAA TGCTCCAAAT GTATCAGACA 
AGGCCAGGCC CAAAAGAATG CAAGGTGGAT GCAGTCACAC ACTTTCACGG TAGCCGCCTC 
AGGGGAACAG AAAACATCTC TGCTGTGAGA AACGTGGTTT TAACACATCT TATCAAGAGG 
CTTCAAGGCT GCTTCAGAGA TGCCAGCCAA GATGTGGTGA GGGCAACCAT GATCGTAAGC 
TTTAAATTGT GGCCCACAAG GATAAATCAA GAATTTGGAG AGAAAGAGGT CTCCATCCTG 
ACTTCACATT ACGAACCAGT ATTAGAAGCC GCCAGCGTGA AAATTGATGA AGTGGACACC 
GAGTGGAGCA TGTTGAAATT AGAGATTTAT GCCAGATTTC AGAACATTCA AAAACTGACC 
TGGGATTTTG TGAATTCCAT TTACTTACAC AAGTATCCAA ATATTCTGAG GCTTGTTGAC 
CTAGTGCTGA CCCTCCCAGC AAGTTCAGCT GAAGCAGAAC GTGGCTTTAG TCAGATGAAA 
CGCATCACGT CACACACGCA TGCTAAAGTC ACATCTGAAA GCATCACGGA TACCCTGATC 
ATTCAGCTGA ACTCTCCAGA CATTAATAAT TTTGACCCTA GGAAAGCTAT CCATTTGTGG 
AATACAAGAA TGCTGTCTTC AGCTGGTGAC ACAGGACCTA ATTCAGATTG CTCCTCAGAC 
TCAGAAAGCC CTTGTGAAAG TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017200164.1
CDS	229..6495
Translation	MSEILCQWLNQELKVSRTVSPKSFAKAFSTGYLIGEVLHKFELQGDFSEFSESRTSTAKLNNFSRLEPTLHLLGVQFDQNVAQSIITEQPGAATKLLYQLYIALQRKKKSGLTGLEMQTMQPLTNLRLQNMRSEAFRERLKILIPRQTDFNLMRVTYRFQEKYRRMREDVDRVHSEKLERMQKLSEEQRCFNIEKQRLSRRRQNEIMAQIQAAIIQIPKPASNHTLKALEAQKMMKKKKGAEDVANEIKKFEALIKKDLQAKESASKTSVDTTGPMTTDLLNTYSDDDYIKKIQKRLEEDAFAREQREKRRRRLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKDVLWQNRIFREKQYEERRLKDFQDALDREAALAKQAKIDFEEQILREKEIHEQIAVERAQARYKKHYAVCAEILDQILDLSTKVADYRMLTNNLIPHKMMHDWKELFFNGKPIYEQASIKQIAAKPTEEQLIELEKRDLLDSNDYDDYKNMVGEWALPEEMAENLPPSNNCILGHVLHRLVEKSLPPQVESTEPELLSSSVKGCVLGKTFSGKTTTLKSLQKDFPVKILSIDTLVQEAIQAYHDNEKVSEASSIPQEDRKEDQLTVRAQLGAKSEQLLKKGKSIPDMLLVNIMVNAINGIPVNQNWILDGFPMTLNQAQLLEETLTGCNRYLMEAEGKAAQISTLAVDPTTSKEVPLPSSAFDFVILLDISDNSSLSRMNDVMAEEFSRVISHEDTSEHAAAESQHVDVDPNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIRINAEIDKNSLCQKVKEIFATEIMKKKNKVEKRLEEKEAEKKAASLAEPPPPTPPLPPEPEKEKETHQQRDPSKTPPAKGKPQSGSPKGKPPGGKVQVKKSPVGSTDIPPTPVAPPPPKPGSEEWVYVNEPIPEEIPSFLVPYWELIENSYINTIKTILRHLREDQHEVLKYLYEITTSFQEFLRRPDHKQDFVAQWQADFNSFPDDLWEDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDSLGITMNHFFSLMQAELNRFQDTKKLLQDYYRAMERKIPIDDSKRFTRIPLIRLDSKDISENQLRIPLVPRISISPETGVPRPKLKAILKGKIDYSLENVQTNFEADERLVMDTWQQASLAISNMVAAEIHQRLIEEDKENQPAESKEKSPQMSTNKKGKKENEPPKNKKEDKHGKGKSPPVAEAAPVVFTAEEIAEIEKKNELRLRIKEEHLAALQFEEIATQFRLELIKSKALSVLEDLITKVVDIYKLMEKWLGERYLNEMSSVEKLTEVARYHIETATKIQNELYLNQEDFFINGDIKVFPDPPLPKRPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGKDPSDAVSQVTKQQKRPQKISHYFPMRYNQFLKIWLKKYPWLVYDDLLNLMFCALCRKHGVKSGGSQMSFFYGTDNFRTEFLNAHHLSEAHAKALLMEATSGSSENRATTELMMRTMSKVTLGRVENLFRSCHAIAKTGRPLKDFIWMCKLDDMKGVDIGPVFRTNKSAQIFTYFIAEVERKSLREKLEKSKFFSVISDGITDSLIKEVKLVYVQFAHAGKVHCQIVGAQTIERKDPLAIKNAIEKTLEINLQLRLSSQDWATKLVGFGSDGPPDIEGEKTEVTLLLREIQPCVQTVYCFAHHLELSYKAVFQSIPLYDSVRELLRSIYHFYHTSPLRKSSLIAAFKDLHLRPTIPSRIEGRRWLNGLQTALQNFLKGYPAIIHQLHSEGEERSDTSQQKAKDLLELLLQDSIVKFAHFLVDVVNVLSILSRVSQSRNSSIADISATMESTLKMLQMYQTRPGPKECKVDAVTHFHGSRLRGTENISAVRNVVLTHLIKRLQGCFRDASQDVVRATMIVSFKLWPTRINQEFGEKEVSILTSHYEPVLEAASVKIDEVDTEWSMLKLEIYARFQNIQKLTWDFVNSIYLHKYPNILRLVDLVLTLPASSAEAERGFSQMKRITSHTHAKVTSESITDTLIIQLNSPDINNFDPRKAIHLWNTRMLSSAGDTGPNSDCSSDSESPCESD

Target ORF information:

RefSeq Version	XM_017344675.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017344675.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCCCG GACCGTGAGC 
CCCAAGTCAT TTGCAAAGGC ATTTTCCACT GGCTATCTAA TTGGAGAAGT TCTGCACAAG 
TTTGAACTCC AGGGTGACTT CTCAGAATTT TCAGAAAGCA GGACTTCAAC TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTG CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCAAA GCATCATCAC AGAACAGCCT GGAGCGGCCA CAAAACTTTT ATATCAATTG 
TACATTGCTC TTCAGAGAAA AAAGAAAAGT GGCCTCACTG GACTAGAAAT GCAAACCATG 
CAGCCCCTGA CAAATTTAAG ACTTCAGAAC ATGAGAAGTG AGGCTTTTCG AGAGAGACTT 
AAAATCCTGA TACCACGTCA AACTGATTTC AATCTGATGC GGGTTACATA CAGGTTCCAA 
GAAAAATATA GACGCATGAG AGAAGATGTT GACCGTGTGC ATTCTGAGAA ACTTGAAAGA 
ATGCAAAAAC TCAGCGAAGA GCAAAGATGT TTTAATATCG AAAAGCAACG CTTAAGCAGA 
AGACGACAAA ATGAAATAAT GGCCCAAATC CAAGCAGCTA TCATACAGAT TCCTAAACCA 
GCATCAAATC ACACTTTGAA AGCACTAGAG GCCCAGAAAA TGATGAAAAA GAAAAAAGGT 
GCAGAGGATG TGGCCAATGA GATTAAGAAG TTTGAAGCTT TAATAAAAAA GGATCTTCAG 
GCAAAAGAAA GTGCCTCCAA GACTTCTGTA GACACAACAG GTCCGATGAC TACCGACTTG 
TTGAACACTT ACTCGGATGA TGACTACATT AAAAAAATCC AGAAGCGCCT AGAAGAAGAT 
GCATTTGCAC GAGAACAAAG AGAGAAAAGG CGGCGGAGAC TGCTAATGGA CCAGTTAATA 
GCGCATGAAG CGCAAGAGGA GGCTTACCGG GAGGAGCAGC TGATTAACCG GCTGATGCGG 
CAGTCCCAGC AGGAGCGCAG GATCGCAGTG CAGCTCATGC ACGTCCGACA TGAGAAGGAT 
GTCCTATGGC AAAACAGGAT CTTCAGAGAA AAGCAGTATG AGGAAAGGCG ACTTAAAGAT 
TTCCAGGATG CACTTGATCG AGAAGCGGCC TTGGCAAAAC AAGCCAAGAT TGATTTCGAA 
GAACAAATCC TTAGAGAGAA GGAAATTCAT GAGCAGATCG CTGTGGAAAG AGCTCAAGCT 
CGTTACAAAA AGCATTATGC AGTGTGTGCA GAAATCTTGG ATCAGATACT TGACTTGTCC 
ACGAAAGTGG CAGATTATCG TATGTTGACA AATAATCTGA TTCCACATAA GATGATGCAT 
GATTGGAAGG AGCTATTTTT TAATGGAAAA CCCATATATG AGCAAGCCTC CATTAAGCAG 
ATAGCTGCTA AACCCACTGA AGAACAACTT ATAGAACTGG AAAAAAGGGA TTTGCTAGAT 
AGCAATGACT ACGACGACTA CAAGAATATG GTTGGGGAGT GGGCCTTACC AGAAGAAATG 
GCTGAAAATT TACCACCTTC CAACAATTGC ATATTAGGCC ATGTGCTTCA CAGACTAGTT 
GAAAAATCTC TTCCTCCTCA AGTTGAATCA ACAGAACCTG AATTACTTTC ATCTTCTGTT 
AAGGGATGCG TATTGGGAAA AACATTTAGT GGGAAAACTA CCACTCTAAA GTCTCTACAA 
AAAGACTTCC CTGTTAAGAT ACTTTCTATT GACACTCTTG TCCAAGAAGC TATCCAAGCA 
TATCATGACA ACGAAAAAGT CAGTGAGGCC TCATCCATTC CTCAAGAGGA TAGGAAAGAA 
GATCAGCTCA CTGTGCGGGC TCAGCTTGGT GCAAAATCAG AACAATTGCT GAAGAAAGGA 
AAGAGCATTC CAGACATGTT GCTGGTTAAC ATCATGGTAA ACGCTATTAA TGGGATACCT 
GTGAATCAGA ACTGGATCCT TGATGGTTTT CCAATGACAT TAAACCAAGC TCAACTTCTG 
GAGGAAACTC TCACAGGCTG CAACAGATAC CTCATGGAAG CAGAAGGAAA AGCAGCACAA 
ATATCTACAC TGGCTGTGGA TCCTACAACT TCCAAAGAAG TACCTCTGCC CTCTTCTGCA 
TTTGATTTTG TTATACTATT AGATATTTCT GATAATTCCT CACTGAGTCG CATGAATGAT 
GTCATGGCGG AAGAATTTTC ACGTGTAATT TCTCATGAAG ATACCAGTGA GCATGCAGCT 
GCTGAGAGCC AGCATGTAGA TGTGGATCCT AATTTGAGAG ACCAGATACA ACACAGGATT 
ATAGGCTTCT TGGACAACTG GCCTTTATTG GAGCAATGGT TTTCAGAGCC AGAAAATATT 
TTGATAAGAA TCAATGCTGA AATAGATAAA AACTCTTTAT GTCAAAAAGT AAAAGAAATA 
TTTGCAACTG AAATAATGAA AAAAAAGAAT AAAGTTGAGA AAAGGTTAGA AGAAAAGGAA 
GCTGAGAAAA AAGCAGCTTC GCTGGCTGAG CCTCCACCCC CTACTCCTCC TCTTCCTCCT 
GAGCCAGAAA AAGAGAAGGA AACTCATCAG CAGCGGGACC CTTCAAAAAC GCCCCCTGCA 
AAAGGAAAAC CACAATCAGG TTCTCCTAAA GGAAAACCCC CAGGAGGAAA AGTCCAAGTG 
AAGAAATCAC CTGTTGGCTC TACAGATATA CCACCTACTC CAGTAGCACC ACCGCCCCCT 
AAGCCAGGAT CTGAAGAATG GGTCTATGTG AATGAACCAA TTCCTGAGGA AATACCTTCA 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCTTACA TAAACACTAT CAAAACCATC 
CTCAGGCATC TGAGAGAAGA CCAGCATGAA GTACTTAAAT ACTTATATGA AATTACAACA 
AGTTTCCAGG AGTTTCTAAG GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCTTTTCC TGATGACTTG TGGGAAGATG AGGAAACCAA GGCTGAACTA 
CACCAAAGAG TGAACGATTT ACGAGACCGC CTGTGGGACA TTTGTGATGC CAGGAAGGAA 
GAGGCTGAGC AGGAACGGCT GGATATCATT AACGAAAGCT GGCTGCAGGA CTCCCTTGGA 
ATCACGATGA ACCACTTCTT CTCACTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACA 
AAGAAACTCC TGCAAGATTA TTATAGGGCA ATGGAACGCA AAATCCCAAT AGATGACAGC 
AAGAGATTTA CTCGAATCCC GTTGATCCGA CTGGATAGTA AAGATATTTC AGAAAACCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCCAG AAACAGGCGT GCCCAGACCA 
AAATTAAAAG CAATACTGAA AGGCAAGATT GATTACTCAC TAGAAAACGT GCAGACAAAC 
TTTGAGGCAG ATGAGAGGTT GGTAATGGAC ACCTGGCAGC AGGCTTCCTT AGCGATATCT 
AACATGGTGG CTGCTGAAAT TCATCAGAGG CTCATAGAAG AAGACAAAGA AAACCAACCA 
GCAGAGTCAA AAGAGAAATC TCCCCAGATG AGTACAAACA AAAAAGGCAA AAAGGAAAAT 
GAGCCACCCA AGAATAAAAA GGAAGACAAA CATGGAAAAG GTAAGTCACC ACCTGTAGCT 
GAAGCTGCTC CTGTAGTGTT CACAGCAGAG GAAATCGCAG AAATTGAAAA GAAAAATGAA 
CTGAGGCTCA GAATAAAGGA GGAGCATCTT GCCGCCCTAC AGTTTGAAGA GATAGCTACA 
CAGTTTCGAC TTGAATTGAT AAAATCCAAA GCATTGTCTG TCCTTGAAGA CCTAATAACG 
AAGGTGGTCG ATATATATAA ACTCATGGAA AAATGGCTTG GTGAGAGATA TTTGAATGAA 
ATGTCCAGTG TAGAAAAATT AACAGAAGTA GCTCGCTATC ACATTGAAAC AGCTACAAAA 
ATCCAGAATG AACTTTACTT AAACCAAGAA GACTTCTTCA TTAATGGTGA TATAAAAGTC 
TTCCCAGATC CACCCCTGCC GAAACGTCCT CCACCTGTAG AAAAGGAAGA AGACGGCACC 
CTGACTATTG AGCAGCTCGA CAATCTTCGA GATCAGTTCT TAGATATGGC GCCTAAAGGC 
AAGGATCCTT CGGATGCTGT GAGTCAAGTC ACCAAACAGC AGAAAAGGCC ACAGAAAATC 
AGCCACTACT TTCCAATGAG ATACAATCAG TTTTTGAAAA TTTGGTTAAA AAAATACCCT 
TGGCTTGTCT ATGATGACTT GTTAAATCTT ATGTTCTGTG CCCTGTGTCG TAAGCATGGA 
GTGAAGTCAG GCGGAAGTCA AATGAGTTTT TTTTATGGCA CAGACAACTT TAGGACTGAA 
TTTCTCAATG CACATCATCT CAGTGAGGCT CATGCCAAGG CTTTATTAAT GGAAGCAACA 
AGTGGTTCTT CAGAGAACAG AGCCACCACA GAACTAATGA TGAGGACCAT GAGCAAAGTA 
ACTCTTGGGA GAGTAGAGAA TTTATTCAGA TCATGCCATG CTATTGCCAA GACTGGACGT 
CCCCTCAAAG ATTTTATCTG GATGTGCAAG TTGGATGACA TGAAGGGTGT TGATATTGGC 
CCAGTATTCA GAACTAACAA GTCAGCACAA ATCTTTACAT ATTTCATTGC TGAAGTAGAG 
CGGAAAAGTC TAAGAGAGAA GCTCGAAAAA AGTAAATTTT TCTCTGTCAT CAGTGATGGA 
ATCACAGACA GTTTAATCAA GGAAGTCAAA CTTGTTTATG TACAGTTTGC ACATGCAGGA 
AAAGTGCATT GTCAAATTGT TGGGGCACAG ACAATAGAAA GGAAAGATCC CCTGGCAATT 
AAAAATGCCA TCGAGAAAAC TCTAGAGATA AATCTGCAAC TTAGGCTATC AAGCCAAGAT 
TGGGCAACGA AACTGGTTGG TTTTGGAAGT GATGGTCCTC CTGACATAGA AGGAGAGAAA 
ACTGAGGTGA CCTTGCTTTT AAGAGAAATC CAGCCATGTG TGCAGACTGT TTATTGCTTT 
GCCCATCACC TTGAACTATC CTACAAGGCA GTGTTCCAGA GCATTCCTCT GTACGACAGT 
GTCAGAGAGC TTCTTCGCAG CATTTATCAC TTCTATCACA CTTCTCCTCT TCGAAAGAGT 
TCTCTGATAG CTGCCTTCAA AGACCTTCAC CTTCGACCCA CAATACCTTC TCGAATAGAA 
GGTAGGAGGT GGCTTAATGG ATTACAGACA GCCCTCCAGA ACTTTCTCAA GGGATATCCT 
GCAATTATCC ACCAATTGCA TTCTGAAGGT GAAGAAAGAA GTGACACCAG CCAACAGAAA 
GCCAAAGACC TGCTTGAGTT GCTGCTCCAA GACAGCATTG TCAAGTTTGC CCATTTCTTA 
GTGGATGTCG TTAACGTCCT CAGCATTCTG TCCCGCGTCA GTCAGAGCAG AAATTCCTCA 
ATTGCTGACA TATCCGCCAC CATGGAGTCC ACACTGAAAA TGCTCCAAAT GTATCAGACA 
AGGCCAGGCC CAAAAGAATG CAAGGTGGAT GCAGTCACAC ACTTTCACGG TAGCCGCCTC 
AGGGGAACAG AAAACATCTC TGCTGTGAGA AACGTGGTTT TAACACATCT TATCAAGAGG 
CTTCAAGGCT GCTTCAGAGA TGCCAGCCAA GATGTGGTGA GGGCAACCAT GATCGTAAGC 
TTTAAATTGT GGCCCACAAG GATAAATCAA GAATTTGGAG AGAAAGAGGT CTCCATCCTG 
ACTTCACATT ACGAACCAGT ATTAGAAGCC GCCAGCGTGA AAATTGATGA AGTGGACACC 
GAGTGGAGCA TGTTGAAATT AGAGATTTAT GCCAGATTTC AGAACATTCA AAAACTGACC 
TGGGATTTTG TGAATTCCAT TTACTTACAC AAGTATCCAA ATATTCTGAG GCTTGTTGAC 
CTAGTGCTGA CCCTCCCAGC AAGTTCAGCT GAAGCAGAAC GTGGCTTTAG TCAGATGAAA 
CGCATCACGT CACACACGCA TGCTAAAGTC ACATCTGAAA GCATCACGGA TACCCTGATC 
ATTCAGCTGA ACTCTCCAGA CATTAATAAT TTTGACCCTA GGAAAGCTAT CCATTTGTGG 
AATACAAGAA TGCTGTCTTC AGCTGGTGAC ACAGGACCTA ATTCAGATTG CTCCTCAGAC 
TCAGAAAGCC CTTGTGAAAG TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPEF2 ( XM_017344676.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_017200165.1 Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

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CloneID	OOb35040
Clone ID Related Accession (Same CDS sequence)	XM_017344676.1
Accession Version	XM_017344676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	sperm flagellar protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013679.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCCCG GACCGTGAGC 
CCCAAGTCAT TTGCAAAGGC ATTTTCCACT GGCTATCTAA TTGGAGAAGT TCTGCACAAG 
TTTGAACTCC AGGGTGACTT CTCAGAATTT TCAGAAAGCA GGACTTCAAC TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTG CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCAAA GCATCATCAC AGAACAGCCT GGAGCGGCCA CAAAACTTTT ATATCAATTG 
TACATTGCTC TTCAGAGAAA AAAGAAAAGT GGCCTCACTG GACTAGAAAT GCAAACCATG 
CAGCCCCTGA CAAATTTAAG ACTTCAGAAC ATGAGAAGTG AGGCTTTTCG AGAGCAACGC 
TTAAGCAGAA GACGACAAAA TGAAATAATG GCCCAAATCC AAGCAGCTAT CATACAGATT 
CCTAAACCAG CATCAAATCA CACTTTGAAA GCACTAGAGG CCCAGAAAAT GATGAAAAAG 
AAAAAAGGTG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG 
GATCTTCAGG CAAAAGAAAG TGCCTCCAAG ACTTCTGTAG ACACAACAGG TCCGATGACT 
ACCGACTTGT TGAACACTTA CTCGGATGAT GACTACATTA AAAAAATCCA GAAGCGCCTA 
GAAGAAGATG CATTTGCACG AGAACAAAGA GAGAAAAGGC GGCGGAGACT GCTAATGGAC 
CAGTTAATAG CGCATGAAGC GCAAGAGGAG GCTTACCGGG AGGAGCAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCGCAGTGC AGCTCATGCA CGTCCGACAT 
GAGAAGGATG TCCTATGGCA AAACAGGATC TTCAGAGAAA AGCAGTATGA GGAAAGGCGA 
CTTAAAGATT TCCAGGATGC ACTTGATCGA GAAGCGGCCT TGGCAAAACA AGCCAAGATT 
GATTTCGAAG AACAAATCCT TAGAGAGAAG GAAATTCATG AGCAGATCGC TGTGGAAAGA 
GCTCAAGCTC GTTACAAAAA GCATTATGCA GTGTGTGCAG AAATCTTGGA TCAGATACTT 
GACTTGTCCA CGAAAGTGGC AGATTATCGT ATGTTGACAA ATAATCTGAT TCCACATAAG 
ATGATGCATG ATTGGAAGGA GCTATTTTTT AATGGAAAAC CCATATATGA GCAAGCCTCC 
ATTAAGCAGA TAGCTGCTAA ACCCACTGAA GAACAACTTA TAGAACTGGA AAAAAGGGAT 
TTGCTAGATA GCAATGACTA CGACGACTAC AAGAATATGG TTGGGGAGTG GGCCTTACCA 
GAAGAAATGG CTGAAAATTT ACCACCTTCC AACAATTGCA TATTAGGCCA TGTGCTTCAC 
AGACTAGTTG AAAAATCTCT TCCTCCTCAA GTTGAATCAA CAGAACCTGA ATTACTTTCA 
TCTTCTGTTA AGGGATGCGT ATTGGGAAAA ACATTTAGTG GGAAAACTAC CACTCTAAAG 
TCTCTACAAA AAGACTTCCC TGTTAAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT ATCATGACAA CGAAAAAGTC AGTGAGGCCT CATCCATTCC TCAAGAGGAT 
AGGAAAGAAG ATCAGCTCAC TGTGCGGGCT CAGCTTGGTG CAAAATCAGA ACAATTGCTG 
AAGAAAGGAA AGAGCATTCC AGACATGTTG CTGGTTAACA TCATGGTAAA CGCTATTAAT 
GGGATACCTG TGAATCAGAA CTGGATCCTT GATGGTTTTC CAATGACATT AAACCAAGCT 
CAACTTCTGG AGGAAACTCT CACAGGCTGC AACAGATACC TCATGGAAGC AGAAGGAAAA 
GCAGCACAAA TATCTACACT GGCTGTGGAT CCTACAACTT CCAAAGAAGT ACCTCTGCCC 
TCTTCTGCAT TTGATTTTGT TATACTATTA GATATTTCTG ATAATTCCTC ACTGAGTCGC 
ATGAATGATG TCATGGCGGA AGAATTTTCA CGTGTAATTT CTCATGAAGA TACCAGTGAG 
CATGCAGCTG CTGAGAGCCA GCATGTAGAT GTGGATCCTA ATTTGAGAGA CCAGATACAA 
CACAGGATTA TAGGCTTCTT GGACAACTGG CCTTTATTGG AGCAATGGTT TTCAGAGCCA 
GAAAATATTT TGATAAGAAT CAATGCTGAA ATAGATAAAA ACTCTTTATG TCAAAAAGTA 
AAAGAAATAT TTGCAACTGA AATAATGAAA AAAAAGAATA AAGTTGAGAA AAGGTTAGAA 
GAAAAGGAAG CTGAGAAAAA AGCAGCTTCG CTGGCTGAGC CTCCACCCCC TACTCCTCCT 
CTTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACTCATCAGC AGCGGGACCC TTCAAAAACG 
CCCCCTGCAA AAGGAAAACC ACAATCAGAA ACTTCACCTG GTAAGCAAGA CTCTCTTCAG 
GATGGAAAAG GAAAGAAAGG TTCTCCTAAA GGAAAACCCC CAGGAGGAAA AGTCCAAGTG 
AAGAAATCAC CTGTTGGCTC TACAGATATA CCACCTACTC CAGTAGCACC ACCGCCCCCT 
AAGCCAGGAT CTGAAGAATG GGTCTATGTG AATGAACCAA TTCCTGAGGA AATACCTTCA 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCTTACA TAAACACTAT CAAAACCATC 
CTCAGGCATC TGAGAGAAGA CCAGCATGAA GTACTTAAAT ACTTATATGA AATTACAACA 
AGTTTCCAGG AGTTTCTAAG GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCTTTTCC TGATGACTTG TGGGAAGATG AGGAAACCAA GGCTGAACTA 
CACCAAAGAG TGAACGATTT ACGAGACCGC CTGTGGGACA TTTGTGATGC CAGGAAGGAA 
GAGGCTGAGC AGGAACGGCT GGATATCATT AACGAAAGCT GGCTGCAGGA CTCCCTTGGA 
ATCACGATGA ACCACTTCTT CTCACTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACA 
AAGAAACTCC TGCAAGATTA TTATAGGGCA ATGGAACGCA AAATCCCAAT AGATGACAGC 
AAGAGATTTA CTCGAATCCC GTTGATCCGA CTGGATAGTA AAGATATTTC AGAAAACCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCCAG AAACAGGCGT GCCCAGACCA 
AAATTAAAAG CAATACTGAA AGGCAAGATT GATTACTCAC TAGAAAACGT GCAGACAAAC 
TTTGAGGCAG ATGAGAGGTT GGTAATGGAC ACCTGGCAGC AGGCTTCCTT AGCGATATCT 
AACATGGTGG CTGCTGAAAT TCATCAGAGG CTCATAGAAG AAGACAAAGA AAACCAACCA 
GCAGAGTCAA AAGAGAAATC TCCCCAGATG AGTACAAACA AAAAAGGCAA AAAGGAAAAT 
GAGCCACCCA AGAATAAAAA GGAAGACAAA CATGGAAAAG GTAAGTCACC ACCTGTAGCT 
GAAGCTGCTC CTGTAGTGTT CACAGCAGAG GAAATCGCAG AAATTGAAAA GAAAAATGAA 
CTGAGGCTCA GAATAAAGGA GGAGCATCTT GCCGCCCTAC AGTTTGAAGA GATAGCTACA 
CAGTTTCGAC TTGAATTGAT AAAATCCAAA GCATTGTCTG TCCTTGAAGA CCTAATAACG 
AAGGTGGTCG ATATATATAA ACTCATGGAA AAATGGCTTG GTGAGAGATA TTTGAATGAA 
ATGTCCAGTG TAGAAAAATT AACAGAAGTA GCTCGCTATC ACATTGAAAC AGCTACAAAA 
ATCCAGAATG AACTTTACTT AAACCAAGAA GACTTCTTCA TTAATGGTGA TATAAAAGTC 
TTCCCAGATC CACCCCTGCC GAAACGTCCT CCACCTGTAG AAAAGGAAGA AGACGGCACC 
CTGACTATTG AGCAGCTCGA CAATCTTCGA GATCAGTTCT TAGATATGGC GCCTAAAGGC 
AAGGATCCTT CGGATGCTGT GAGTCAAGTC ACCAAACAGC AGAAAAGGCC ACAGAAAATC 
AGCCACTACT TTCCAATGAG ATACAATCAG TTTTTGAAAA TTTGGTTAAA AAAATACCCT 
TGGCTTGTCT ATGATGACTT GTTAAATCTT ATGTTCTGTG CCCTGTGTCG TAAGCATGGA 
GTGAAGTCAG GCGGAAGTCA AATGAGTTTT TTTTATGGCA CAGACAACTT TAGGACTGAA 
TTTCTCAATG CACATCATCT CAGTGAGGCT CATGCCAAGG CTTTATTAAT GGAAGCAACA 
AGTGGTTCTT CAGAGAACAG AGCCACCACA GAACTAATGA TGAGGACCAT GAGCAAAGTA 
ACTCTTGGGA GAGTAGAGAA TTTATTCAGA TCATGCCATG CTATTGCCAA GACTGGACGT 
CCCCTCAAAG ATTTTATCTG GATGTGCAAG TTGGATGACA TGAAGGGTGT TGATATTGGC 
CCAGTATTCA GAACTAACAA GTCAGCACAA ATCTTTACAT ATTTCATTGC TGAAGTAGAG 
CGGAAAAGTC TAAGAGAGAA GCTCGAAAAA AGTAAATTTT TCTCTGTCAT CAGTGATGGA 
ATCACAGACA GTTTAATCAA GGAAGTCAAA CTTGTTTATG TACAGTTTGC ACATGCAGGA 
AAAGTGCATT GTCAAATTGT TGGGGCACAG ACAATAGAAA GGAAAGATCC CCTGGCAATT 
AAAAATGCCA TCGAGAAAAC TCTAGAGATA AATCTGCAAC TTAGGCTATC AAGCCAAGAT 
TGGGCAACGA AACTGGTTGG TTTTGGAAGT GATGGTCCTC CTGACATAGA AGGAGAGAAA 
ACTGAGGTGA CCTTGCTTTT AAGAGAAATC CAGCCATGTG TGCAGACTGT TTATTGCTTT 
GCCCATCACC TTGAACTATC CTACAAGGCA GTGTTCCAGA GCATTCCTCT GTACGACAGT 
GTCAGAGAGC TTCTTCGCAG CATTTATCAC TTCTATCACA CTTCTCCTCT TCGAAAGAGT 
TCTCTGATAG CTGCCTTCAA AGACCTTCAC CTTCGACCCA CAATACCTTC TCGAATAGAA 
GGTAGGAGGT GGCTTAATGG ATTACAGACA GCCCTCCAGA ACTTTCTCAA GGGATATCCT 
GCAATTATCC ACCAATTGCA TTCTGAAGGT GAAGAAAGAA GTGACACCAG CCAACAGAAA 
GCCAAAGACC TGCTTGAGTT GCTGCTCCAA GACAGCATTG TCAAGTTTGC CCATTTCTTA 
GTGGATGTCG TTAACGTCCT CAGCATTCTG TCCCGCGTCA GTCAGAGCAG AAATTCCTCA 
ATTGCTGACA TATCCGCCAC CATGGAGTCC ACACTGAAAA TGCTCCAAAT GTATCAGACA 
AGGCCAGGCC CAAAAGAATG CAAGGTGGAT GCAGTCACAC ACTTTCACGG TAGCCGCCTC 
AGGGGAACAG AAAACATCTC TGCTGTGAGA AACGTGGTTT TAACACATCT TATCAAGAGG 
CTTCAAGGCT GCTTCAGAGA TGCCAGCCAA GATGTGGTGA GGGCAACCAT GATCGTAAGC 
TTTAAATTGT GGCCCACAAG GATAAATCAA GAATTTGGAG AGAAAGAGGT CTCCATCCTG 
ACTTCACATT ACGAACCAGT ATTAGAAGCC GCCAGCGTGA AAATTGATGA AGTGGACACC 
GAGTGGAGCA TGTTGAAATT AGAGATTTAT GCCAGATTTC AGAACATTCA AAAACTGACC 
TGGGATTTTG TGAATTCCAT TTACTTACAC AAGTATCCAA ATATTCTGAG GCTTGTTGAC 
CTAGTGCTGA CCCTCCCAGC AAGTTCAGCT GAAGCAGAAC GTGGCTTTAG TCAGATGAAA 
CGCATCACGT CACACACGCA TGCTAAAGTC ACATCTGAAA GCATCACGGA TACCCTGATC 
ATTCAGCTGA ACTCTCCAGA CATTAATAAT TTTGACCCTA GGAAAGCTAT CCATTTGTGG 
AATACAAGAA TGCTGTCTTC AGCTGGTGAC ACAGGACCTA ATTCAGATTG CTCCTCAGAC 
TCAGAAAGCC CTTGTGAAAG TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017200165.1
CDS	228..6374
Translation	MSEILCQWLNQELKVSRTVSPKSFAKAFSTGYLIGEVLHKFELQGDFSEFSESRTSTAKLNNFSRLEPTLHLLGVQFDQNVAQSIITEQPGAATKLLYQLYIALQRKKKSGLTGLEMQTMQPLTNLRLQNMRSEAFREQRLSRRRQNEIMAQIQAAIIQIPKPASNHTLKALEAQKMMKKKKGAEDVANEIKKFEALIKKDLQAKESASKTSVDTTGPMTTDLLNTYSDDDYIKKIQKRLEEDAFAREQREKRRRRLLMDQLIAHEAQEEAYREEQLINRLMRQSQQERRIAVQLMHVRHEKDVLWQNRIFREKQYEERRLKDFQDALDREAALAKQAKIDFEEQILREKEIHEQIAVERAQARYKKHYAVCAEILDQILDLSTKVADYRMLTNNLIPHKMMHDWKELFFNGKPIYEQASIKQIAAKPTEEQLIELEKRDLLDSNDYDDYKNMVGEWALPEEMAENLPPSNNCILGHVLHRLVEKSLPPQVESTEPELLSSSVKGCVLGKTFSGKTTTLKSLQKDFPVKILSIDTLVQEAIQAYHDNEKVSEASSIPQEDRKEDQLTVRAQLGAKSEQLLKKGKSIPDMLLVNIMVNAINGIPVNQNWILDGFPMTLNQAQLLEETLTGCNRYLMEAEGKAAQISTLAVDPTTSKEVPLPSSAFDFVILLDISDNSSLSRMNDVMAEEFSRVISHEDTSEHAAAESQHVDVDPNLRDQIQHRIIGFLDNWPLLEQWFSEPENILIRINAEIDKNSLCQKVKEIFATEIMKKKNKVEKRLEEKEAEKKAASLAEPPPPTPPLPPEPEKEKETHQQRDPSKTPPAKGKPQSETSPGKQDSLQDGKGKKGSPKGKPPGGKVQVKKSPVGSTDIPPTPVAPPPPKPGSEEWVYVNEPIPEEIPSFLVPYWELIENSYINTIKTILRHLREDQHEVLKYLYEITTSFQEFLRRPDHKQDFVAQWQADFNSFPDDLWEDEETKAELHQRVNDLRDRLWDICDARKEEAEQERLDIINESWLQDSLGITMNHFFSLMQAELNRFQDTKKLLQDYYRAMERKIPIDDSKRFTRIPLIRLDSKDISENQLRIPLVPRISISPETGVPRPKLKAILKGKIDYSLENVQTNFEADERLVMDTWQQASLAISNMVAAEIHQRLIEEDKENQPAESKEKSPQMSTNKKGKKENEPPKNKKEDKHGKGKSPPVAEAAPVVFTAEEIAEIEKKNELRLRIKEEHLAALQFEEIATQFRLELIKSKALSVLEDLITKVVDIYKLMEKWLGERYLNEMSSVEKLTEVARYHIETATKIQNELYLNQEDFFINGDIKVFPDPPLPKRPPPVEKEEDGTLTIEQLDNLRDQFLDMAPKGKDPSDAVSQVTKQQKRPQKISHYFPMRYNQFLKIWLKKYPWLVYDDLLNLMFCALCRKHGVKSGGSQMSFFYGTDNFRTEFLNAHHLSEAHAKALLMEATSGSSENRATTELMMRTMSKVTLGRVENLFRSCHAIAKTGRPLKDFIWMCKLDDMKGVDIGPVFRTNKSAQIFTYFIAEVERKSLREKLEKSKFFSVISDGITDSLIKEVKLVYVQFAHAGKVHCQIVGAQTIERKDPLAIKNAIEKTLEINLQLRLSSQDWATKLVGFGSDGPPDIEGEKTEVTLLLREIQPCVQTVYCFAHHLELSYKAVFQSIPLYDSVRELLRSIYHFYHTSPLRKSSLIAAFKDLHLRPTIPSRIEGRRWLNGLQTALQNFLKGYPAIIHQLHSEGEERSDTSQQKAKDLLELLLQDSIVKFAHFLVDVVNVLSILSRVSQSRNSSIADISATMESTLKMLQMYQTRPGPKECKVDAVTHFHGSRLRGTENISAVRNVVLTHLIKRLQGCFRDASQDVVRATMIVSFKLWPTRINQEFGEKEVSILTSHYEPVLEAASVKIDEVDTEWSMLKLEIYARFQNIQKLTWDFVNSIYLHKYPNILRLVDLVLTLPASSAEAERGFSQMKRITSHTHAKVTSESITDTLIIQLNSPDINNFDPRKAIHLWNTRMLSSAGDTGPNSDCSSDSESPCESD

Target ORF information:

RefSeq Version	XM_017344676.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus sperm flagellar 2 (SPEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017344676.1

ORF Insert Sequence:

ATGTCGGAGA TCCTGTGCCA GTGGCTCAAC CAGGAGTTGA AGGTGTCCCG GACCGTGAGC 
CCCAAGTCAT TTGCAAAGGC ATTTTCCACT GGCTATCTAA TTGGAGAAGT TCTGCACAAG 
TTTGAACTCC AGGGTGACTT CTCAGAATTT TCAGAAAGCA GGACTTCAAC TGCCAAACTT 
AATAATTTTT CTCGCTTGGA GCCAACACTG CACCTTCTGG GTGTGCAGTT TGATCAGAAT 
GTGGCCCAAA GCATCATCAC AGAACAGCCT GGAGCGGCCA CAAAACTTTT ATATCAATTG 
TACATTGCTC TTCAGAGAAA AAAGAAAAGT GGCCTCACTG GACTAGAAAT GCAAACCATG 
CAGCCCCTGA CAAATTTAAG ACTTCAGAAC ATGAGAAGTG AGGCTTTTCG AGAGCAACGC 
TTAAGCAGAA GACGACAAAA TGAAATAATG GCCCAAATCC AAGCAGCTAT CATACAGATT 
CCTAAACCAG CATCAAATCA CACTTTGAAA GCACTAGAGG CCCAGAAAAT GATGAAAAAG 
AAAAAAGGTG CAGAGGATGT GGCCAATGAG ATTAAGAAGT TTGAAGCTTT AATAAAAAAG 
GATCTTCAGG CAAAAGAAAG TGCCTCCAAG ACTTCTGTAG ACACAACAGG TCCGATGACT 
ACCGACTTGT TGAACACTTA CTCGGATGAT GACTACATTA AAAAAATCCA GAAGCGCCTA 
GAAGAAGATG CATTTGCACG AGAACAAAGA GAGAAAAGGC GGCGGAGACT GCTAATGGAC 
CAGTTAATAG CGCATGAAGC GCAAGAGGAG GCTTACCGGG AGGAGCAGCT GATTAACCGG 
CTGATGCGGC AGTCCCAGCA GGAGCGCAGG ATCGCAGTGC AGCTCATGCA CGTCCGACAT 
GAGAAGGATG TCCTATGGCA AAACAGGATC TTCAGAGAAA AGCAGTATGA GGAAAGGCGA 
CTTAAAGATT TCCAGGATGC ACTTGATCGA GAAGCGGCCT TGGCAAAACA AGCCAAGATT 
GATTTCGAAG AACAAATCCT TAGAGAGAAG GAAATTCATG AGCAGATCGC TGTGGAAAGA 
GCTCAAGCTC GTTACAAAAA GCATTATGCA GTGTGTGCAG AAATCTTGGA TCAGATACTT 
GACTTGTCCA CGAAAGTGGC AGATTATCGT ATGTTGACAA ATAATCTGAT TCCACATAAG 
ATGATGCATG ATTGGAAGGA GCTATTTTTT AATGGAAAAC CCATATATGA GCAAGCCTCC 
ATTAAGCAGA TAGCTGCTAA ACCCACTGAA GAACAACTTA TAGAACTGGA AAAAAGGGAT 
TTGCTAGATA GCAATGACTA CGACGACTAC AAGAATATGG TTGGGGAGTG GGCCTTACCA 
GAAGAAATGG CTGAAAATTT ACCACCTTCC AACAATTGCA TATTAGGCCA TGTGCTTCAC 
AGACTAGTTG AAAAATCTCT TCCTCCTCAA GTTGAATCAA CAGAACCTGA ATTACTTTCA 
TCTTCTGTTA AGGGATGCGT ATTGGGAAAA ACATTTAGTG GGAAAACTAC CACTCTAAAG 
TCTCTACAAA AAGACTTCCC TGTTAAGATA CTTTCTATTG ACACTCTTGT CCAAGAAGCT 
ATCCAAGCAT ATCATGACAA CGAAAAAGTC AGTGAGGCCT CATCCATTCC TCAAGAGGAT 
AGGAAAGAAG ATCAGCTCAC TGTGCGGGCT CAGCTTGGTG CAAAATCAGA ACAATTGCTG 
AAGAAAGGAA AGAGCATTCC AGACATGTTG CTGGTTAACA TCATGGTAAA CGCTATTAAT 
GGGATACCTG TGAATCAGAA CTGGATCCTT GATGGTTTTC CAATGACATT AAACCAAGCT 
CAACTTCTGG AGGAAACTCT CACAGGCTGC AACAGATACC TCATGGAAGC AGAAGGAAAA 
GCAGCACAAA TATCTACACT GGCTGTGGAT CCTACAACTT CCAAAGAAGT ACCTCTGCCC 
TCTTCTGCAT TTGATTTTGT TATACTATTA GATATTTCTG ATAATTCCTC ACTGAGTCGC 
ATGAATGATG TCATGGCGGA AGAATTTTCA CGTGTAATTT CTCATGAAGA TACCAGTGAG 
CATGCAGCTG CTGAGAGCCA GCATGTAGAT GTGGATCCTA ATTTGAGAGA CCAGATACAA 
CACAGGATTA TAGGCTTCTT GGACAACTGG CCTTTATTGG AGCAATGGTT TTCAGAGCCA 
GAAAATATTT TGATAAGAAT CAATGCTGAA ATAGATAAAA ACTCTTTATG TCAAAAAGTA 
AAAGAAATAT TTGCAACTGA AATAATGAAA AAAAAGAATA AAGTTGAGAA AAGGTTAGAA 
GAAAAGGAAG CTGAGAAAAA AGCAGCTTCG CTGGCTGAGC CTCCACCCCC TACTCCTCCT 
CTTCCTCCTG AGCCAGAAAA AGAGAAGGAA ACTCATCAGC AGCGGGACCC TTCAAAAACG 
CCCCCTGCAA AAGGAAAACC ACAATCAGAA ACTTCACCTG GTAAGCAAGA CTCTCTTCAG 
GATGGAAAAG GAAAGAAAGG TTCTCCTAAA GGAAAACCCC CAGGAGGAAA AGTCCAAGTG 
AAGAAATCAC CTGTTGGCTC TACAGATATA CCACCTACTC CAGTAGCACC ACCGCCCCCT 
AAGCCAGGAT CTGAAGAATG GGTCTATGTG AATGAACCAA TTCCTGAGGA AATACCTTCA 
TTTTTAGTAC CTTACTGGGA ACTAATAGAA AATTCTTACA TAAACACTAT CAAAACCATC 
CTCAGGCATC TGAGAGAAGA CCAGCATGAA GTACTTAAAT ACTTATATGA AATTACAACA 
AGTTTCCAGG AGTTTCTAAG GCGTCCAGAT CACAAGCAAG ATTTTGTAGC TCAATGGCAG 
GCTGATTTCA ACTCTTTTCC TGATGACTTG TGGGAAGATG AGGAAACCAA GGCTGAACTA 
CACCAAAGAG TGAACGATTT ACGAGACCGC CTGTGGGACA TTTGTGATGC CAGGAAGGAA 
GAGGCTGAGC AGGAACGGCT GGATATCATT AACGAAAGCT GGCTGCAGGA CTCCCTTGGA 
ATCACGATGA ACCACTTCTT CTCACTGATG CAGGCAGAAC TGAACCGTTT CCAAGATACA 
AAGAAACTCC TGCAAGATTA TTATAGGGCA ATGGAACGCA AAATCCCAAT AGATGACAGC 
AAGAGATTTA CTCGAATCCC GTTGATCCGA CTGGATAGTA AAGATATTTC AGAAAACCAG 
CTTAGAATTC CTCTAGTTCC TCGAATATCC ATTTCTCCAG AAACAGGCGT GCCCAGACCA 
AAATTAAAAG CAATACTGAA AGGCAAGATT GATTACTCAC TAGAAAACGT GCAGACAAAC 
TTTGAGGCAG ATGAGAGGTT GGTAATGGAC ACCTGGCAGC AGGCTTCCTT AGCGATATCT 
AACATGGTGG CTGCTGAAAT TCATCAGAGG CTCATAGAAG AAGACAAAGA AAACCAACCA 
GCAGAGTCAA AAGAGAAATC TCCCCAGATG AGTACAAACA AAAAAGGCAA AAAGGAAAAT 
GAGCCACCCA AGAATAAAAA GGAAGACAAA CATGGAAAAG GTAAGTCACC ACCTGTAGCT 
GAAGCTGCTC CTGTAGTGTT CACAGCAGAG GAAATCGCAG AAATTGAAAA GAAAAATGAA 
CTGAGGCTCA GAATAAAGGA GGAGCATCTT GCCGCCCTAC AGTTTGAAGA GATAGCTACA 
CAGTTTCGAC TTGAATTGAT AAAATCCAAA GCATTGTCTG TCCTTGAAGA CCTAATAACG 
AAGGTGGTCG ATATATATAA ACTCATGGAA AAATGGCTTG GTGAGAGATA TTTGAATGAA 
ATGTCCAGTG TAGAAAAATT AACAGAAGTA GCTCGCTATC ACATTGAAAC AGCTACAAAA 
ATCCAGAATG AACTTTACTT AAACCAAGAA GACTTCTTCA TTAATGGTGA TATAAAAGTC 
TTCCCAGATC CACCCCTGCC GAAACGTCCT CCACCTGTAG AAAAGGAAGA AGACGGCACC 
CTGACTATTG AGCAGCTCGA CAATCTTCGA GATCAGTTCT TAGATATGGC GCCTAAAGGC 
AAGGATCCTT CGGATGCTGT GAGTCAAGTC ACCAAACAGC AGAAAAGGCC ACAGAAAATC 
AGCCACTACT TTCCAATGAG ATACAATCAG TTTTTGAAAA TTTGGTTAAA AAAATACCCT 
TGGCTTGTCT ATGATGACTT GTTAAATCTT ATGTTCTGTG CCCTGTGTCG TAAGCATGGA 
GTGAAGTCAG GCGGAAGTCA AATGAGTTTT TTTTATGGCA CAGACAACTT TAGGACTGAA 
TTTCTCAATG CACATCATCT CAGTGAGGCT CATGCCAAGG CTTTATTAAT GGAAGCAACA 
AGTGGTTCTT CAGAGAACAG AGCCACCACA GAACTAATGA TGAGGACCAT GAGCAAAGTA 
ACTCTTGGGA GAGTAGAGAA TTTATTCAGA TCATGCCATG CTATTGCCAA GACTGGACGT 
CCCCTCAAAG ATTTTATCTG GATGTGCAAG TTGGATGACA TGAAGGGTGT TGATATTGGC 
CCAGTATTCA GAACTAACAA GTCAGCACAA ATCTTTACAT ATTTCATTGC TGAAGTAGAG 
CGGAAAAGTC TAAGAGAGAA GCTCGAAAAA AGTAAATTTT TCTCTGTCAT CAGTGATGGA 
ATCACAGACA GTTTAATCAA GGAAGTCAAA CTTGTTTATG TACAGTTTGC ACATGCAGGA 
AAAGTGCATT GTCAAATTGT TGGGGCACAG ACAATAGAAA GGAAAGATCC CCTGGCAATT 
AAAAATGCCA TCGAGAAAAC TCTAGAGATA AATCTGCAAC TTAGGCTATC AAGCCAAGAT 
TGGGCAACGA AACTGGTTGG TTTTGGAAGT GATGGTCCTC CTGACATAGA AGGAGAGAAA 
ACTGAGGTGA CCTTGCTTTT AAGAGAAATC CAGCCATGTG TGCAGACTGT TTATTGCTTT 
GCCCATCACC TTGAACTATC CTACAAGGCA GTGTTCCAGA GCATTCCTCT GTACGACAGT 
GTCAGAGAGC TTCTTCGCAG CATTTATCAC TTCTATCACA CTTCTCCTCT TCGAAAGAGT 
TCTCTGATAG CTGCCTTCAA AGACCTTCAC CTTCGACCCA CAATACCTTC TCGAATAGAA 
GGTAGGAGGT GGCTTAATGG ATTACAGACA GCCCTCCAGA ACTTTCTCAA GGGATATCCT 
GCAATTATCC ACCAATTGCA TTCTGAAGGT GAAGAAAGAA GTGACACCAG CCAACAGAAA 
GCCAAAGACC TGCTTGAGTT GCTGCTCCAA GACAGCATTG TCAAGTTTGC CCATTTCTTA 
GTGGATGTCG TTAACGTCCT CAGCATTCTG TCCCGCGTCA GTCAGAGCAG AAATTCCTCA 
ATTGCTGACA TATCCGCCAC CATGGAGTCC ACACTGAAAA TGCTCCAAAT GTATCAGACA 
AGGCCAGGCC CAAAAGAATG CAAGGTGGAT GCAGTCACAC ACTTTCACGG TAGCCGCCTC 
AGGGGAACAG AAAACATCTC TGCTGTGAGA AACGTGGTTT TAACACATCT TATCAAGAGG 
CTTCAAGGCT GCTTCAGAGA TGCCAGCCAA GATGTGGTGA GGGCAACCAT GATCGTAAGC 
TTTAAATTGT GGCCCACAAG GATAAATCAA GAATTTGGAG AGAAAGAGGT CTCCATCCTG 
ACTTCACATT ACGAACCAGT ATTAGAAGCC GCCAGCGTGA AAATTGATGA AGTGGACACC 
GAGTGGAGCA TGTTGAAATT AGAGATTTAT GCCAGATTTC AGAACATTCA AAAACTGACC 
TGGGATTTTG TGAATTCCAT TTACTTACAC AAGTATCCAA ATATTCTGAG GCTTGTTGAC 
CTAGTGCTGA CCCTCCCAGC AAGTTCAGCT GAAGCAGAAC GTGGCTTTAG TCAGATGAAA 
CGCATCACGT CACACACGCA TGCTAAAGTC ACATCTGAAA GCATCACGGA TACCCTGATC 
ATTCAGCTGA ACTCTCCAGA CATTAATAAT TTTGACCCTA GGAAAGCTAT CCATTTGTGG 
AATACAAGAA TGCTGTCTTC AGCTGGTGAC ACAGGACCTA ATTCAGATTG CTCCTCAGAC 
TCAGAAAGCC CTTGTGAAAG TGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SPEF2 cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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