TJP1 cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	TJP1
Entrez Gene ID	100346390
Full Name	tight junction protein 1
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017348359.1	XP_017203848.1	tight junction protein ZO-1 isoform X3
XM_008269782.1	XP_008268004.1	tight junction protein ZO-1 isoform X5
XM_017348357.1	XP_017203846.1	tight junction protein ZO-1 isoform X1
XM_017348358.1	XP_017203847.1	tight junction protein ZO-1 isoform X2
XM_017348360.1	XP_017203849.1	tight junction protein ZO-1 isoform X4

Pathways from BioSystems
Gene Ontology

The following TJP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOb18836	XM_008269782.1 Latest version!	Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOb37767	XM_017348357.1 Latest version!	Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOb37768	XM_017348358.1 Latest version!	Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOb37769	XM_017348359.1 Latest version!	Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOb37770	XM_017348360.1 Latest version!	Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TJP1 ( XM_008269782.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_008268004.1 Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OOb18836
Clone ID Related Accession (Same CDS sequence)	XM_008269782.1
Accession Version	XM_008269782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013685.1) and transcript sequence (GBCI01037002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 477 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCCGCCA GAGTGGCGGC CGCCAAGAAC ACGGCCATGG AGGAAACAGC TATATGGGAA 
CAACACACAG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCGGGT 
GGAAGAGATA ACCCTCATTT TCAGAGTGGA GAGACCTCCA TAGTAATTTC AGATGTGCTG 
AAGGGAGGAC CAGCCGAGGG GCAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGG 
GTTTCCATGG ATAACGTTGA ACATGCCTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAGA TTACCATTCG ACGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TGTCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATCCA TGACCCGAGA 
AGTGGCCGCA GTGGTCTGGC AAACAGAAGG AGTGAGAAGA GTTGGGCAAG GGATAGAAGT 
GCGAGTCGAG AGAGGAGCTT GTCTCCGCGA TCAGATAGGC GGTCGGTGGC CTCTAGCCAG 
CCTGCGAAAC CCACCAAAGT CACTTTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCACATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAACATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACGGTGAC AGAAAATATG 
TCATTGACTG ATGCGAAGAC GTTGATAGAA AGGTCTAAAG GCAAGTTGAA AATGGTAGTC 
CAGAGAGATG AACGGGCGAC CTTGCTGAAC GTCCCTGACC TTTCTGACAG CATCCATTCT 
GCTAATGCCT CCGAGAGAGA TGACATTTCA GAAATCCAGT CACTGGCATC AGATCATTCT 
GGCCGGTCAC ATGACAGGCC TCCACGCCGC AGCAGCCGGT CCCGCTCTCC TGACCAGCGG 
TCTGAGCCTT CCGACCACTC CAGACAGTCT CCGCAGCAGC CCAGCAATGG CAGTCTCCGG 
AGCAGAGAAG AAGAGAGAAT TTCTAAGCCT GGGGCTGTCT CGACTCCTGT AAAGCATGGA 
GATGACCACA CACCTAAAAC AGTGGAAGAA ATTACTGTTG AGAGAAATGA GAAGCAAACA 
CCTGCCCTTC CAGAACCAAA GCCTGTGTAC GCTCAAGTAG GACAACCAGA CGTGGATTTA 
CCTGTCAGCC CATCTGATGG TGTCCTGCCT AACTCAGCGC ACGAGGACGG GATGCTTCGG 
CCCAGCATGA AATTGGTAAA ATTCAGAAAA GGAGATAGCG TGGGTCTGCG GCTGGCTGGT 
GGAAATGATG TCGGAATATT TGTGGCTGGC GTTCTGGAAG ATAGCCCTGC TGCTAAAGAA 
GGCTTAGAGG AGGGTGATCA GATTCTCAGG GTAAACAATG TGGATTTCAC CAATATCATT 
AGAGAAGAAG CTGTCCTTTT CCTGCTTGAC CTCCCTAAAG GAGAAGAAGT GACCATCCTG 
GCCCAGAAGA AGAAGGATGT TTATCGTCGC ATTGTAGAAT CAGATGTTGG AGATTCTTTC 
TATATTAGAA CCCATTTTGA ATACGAAAAG GAATCTCCCT ATGGACTTAG TTTTAACAAG 
GGAGAGGTAT TTCGTGTTGT GGATACCTTG TACAATGGAA AACTGGGCTC TTGGCTTGCT 
ATTCGAATTG GCAAAAATCA TAAGGAGGTG GAACGAGGCA TCATCCCTAA TAAGAACAGA 
GCTGAACAGC TGGCCAGTGT GCAGTATACA CTTCCCAAAA CTGCGGGGGG CGATCGTGCG 
GACTTCTGGA GGTTCCGGGG TCTCCGCAGC TCCAAGAGGA ATCTTCGGAA AAGCAGAGAG 
GATTTGTCAG CCCAGCCAGT TCAGACGAAA TTTCCAGCTT ATGAAAGGGT TGTTCTTCGA 
GAAGCTGGAT TCCTGAGGCC TGTAACCATC TTTGGACCCA TAGCCGATGT TGCCAGAGAA 
AAGCTGGCCA GAGAAGAGCC AGATATTTAT CAGATTGCAA AAAGTGAACC GCGAGATGCT 
GGAACTGACC AACGTAGCTC TGGCATTATT CGACTTCATA CAATAAAGCA AATAATAGAT 
CAAGACAAGC ATGCCTTATT GGACGTAACA CCAAATGCAG TAGATCGTCT TAATTATGCC 
CAGTGGTATC CAATTGTTGT TTTCCTTAAC CCTGATTCTA AGCAAGGTGT GAAAACAATG 
AGAATGAGGT TATGTCCAGA ATCTCGGAAA AGTGCCAGGA AGTTATATGA GCGCTCTCAC 
AAACTCCGTA AAAACAATCA CCATCTTTTC ACAACTACAA TTAACTTAAA TTCAATGAAC 
GATGGCTGGT ATGGTGCGTT GAAGGAAGCA ATCCAGCAAC AGCAGAACCA GCTGGTATGG 
GTTTCTGAGG GAAAGGCGGA TGGTGCTACA AGTGATGACC TTGATTTGCA TGATGATCGT 
CTGTCCTACC TGTCAGCCCC AGGTAGTGAA TACTCAATGT ATAGCACGGA CAGTAGACAC 
ACTTCTGACT ATGAAGACAC AGATACAGAA GGCGGGGCCT ACACTGATCA AGAACTAGAT 
GAAACTCTTA ATGATGAGGT TGGGACTCCA CCGGAGTCTG CCATTACACG GTCCTCTGAG 
CCTGTAAGAG AGGACTCCTC TGGGATGCAT CATGAAAACC AAACATATCC TCCCTACTCA 
CCACAAGCGC AGCCACAGCC AGTTCCTAGA ATAGACTCCC CGGGATTTAA GACAGCCTCT 
CAACAGGTCT ATAGAAAGGA TCCGTATCCT GAGGAAGTGA TGAGACAGAA TCCAGCTTTG 
AAACAGCCAG CTGTTGGTCA CCCAGGGCAG AGGCCTGAGA AAGAGCCCAG TCTGACCTAT 
GAGCCCCAGC TTCCCTACAT GGAGAAACAA GCCAGCAGAG ACCTCGAGCA GCCCACATAC 
AGATACGAGT CCTCCGGCTA CACAGACCCG TTTTCTCGAA ACTATGACCA TCGCCTGCGA 
TACGAGGACC GCATGCCTGC ATATGAAGAA CAGTGGTCGT ACTACGATGA CAAACAGCCC 
TACCAGCCTC GGCCGCCTTT CGATAGTCAG CACCCTCGAG ACCTTGACCC TAGACAGCAC 
CCTGACGAGC CCTCAGAACG AGGCTATTTC CCACGTTTTG AAGAGCCAGC CCCCCTGTCT 
TATGACAGTC GGCCACGCTA CGAACAGCCG CCCAGAACCT CCACCCTGCG GCATGAAGAG 
CAGCCAGCTC CGGGCTATGA TGTGCACAAC AGACAGAGAC CAGAAGCGCA GCCCTATCCT 
GCAGCAGCCC CTAAGTCGGC GGAGCCCAAG CAGTACTTTG ACCAGTACGG GCGAAGCTAC 
GAGCAAATCC CGCCTCAAGG GTTTGCCTCC AGAACAGGCC ACTACGAACC TCTCCACAGC 
GCCGCAGTCG TCCCTCCGCT CATACCTTCC TCTCAGCATA AGCCAGAAGT CCTGCCTTCC 
AGCACCAAGC CACTGCCCCC ACCTCCAGCC CTAACCGAGG AGGACGAGGA CCCAGCGATG 
AAACCACAGT CTGTGCTCAC CCGAGTGAAA ATGTTTGAAA ACAAAAGGTC TGCATCCCTG 
GAGAACAAGA AGGAGGTGAA TGACACGGCC AGTTTTAAGC CTCCAGAAGC ATCCAAACTT 
CCAGGTGCTC CCGTCACTGG GCCTAAATCC ACCCCTCAGA GCCAGTTCAG TGAACATGAC 
AAAACTTTGT ACAGGATCCC AGAGCCTCAA AAACCGCAAA TGAAGCCTCC TGAAGATATT 
GTCCGGTCAA ATCATTACGA TCCGGAAGAA GATGAAGAGT ATTACCGAAA GCAGCTCTCG 
TACTTTGACC GGAGAAGTTT CGAGAACAAG CCTGCGGCAC ACATTCCTGC CAGCCATCTC 
TCAGAGCCTG CCAAGCCGGC TCATTCTCAG AACCAACCAA ATTTTTCTAG TTATTCTTCA 
AAGGGGAAAG CTGAAGCTGA TGCTCTGGAC CGATCGTTTG GTGAGAAGCG CTATGAGCCA 
GTGCAGCCCA CCCCTCCCCC TCCCCCACTG CCCTCGCAGT ACTCGCAGGC GGCCCAGCCT 
GGCGCCAGCT CTTCTCTCCA CATGCACTCG AAGGGGATAC ATGGTGAAGG TAATTCAATG 
TCATTGGATT TTCAGAATTC TTTGGTATCC AAACCAGACC CACCTCCATC TCAGAGTAAG 
CCAGCAGCTT TCAGAGCACC GCACCGAGAC GATGCCGCTC AGCCTGCTTT ATATCCCCAG 
AAAAGCTTCC CGGAGAAAGC CCCCGTTAAT GGAGCTGAAC AGACTCAGAA AACCATCACT 
CCAGCCTACA ACCGATTCAC ACCAAAACCA TATACCAGTT CTGCCCGGCC ATTCGAGCGC 
AAATTTGAAA GTCCTAAGTT CAACCACAAT CTTCTGCCAG GTGACACTGT ACATAAACCT 
GACTTGTCTG CAAAAGCCCC AGCTTCTCCA AAAACTCTTA TGAAAGCGCA CAGTTCCACA 
CAGCCTCCTG AGTTTGACAG TGGAGTGGAA ACTTTCTCCA TCCATGCAGA GAAGCCCAAG 
TACCAAGTAA CTAATATCAG CACAGTGCCT AGAGCTGCTC CTGTGGGCCC ATCCGCTGTG 
GAAGAAGATG AAGACGAGGA TGGGCACACT GTGGTGGCCA CAGCCCGAGG CATATTTAAC 
AGCAATGGTG GGGTTTTGAG CTCCATAGAG ACCGGCGTCA GTATAATTAT CCCGCAAGGA 
GCCATTCCCG AAGGAATTGA GCAGGAAATC TATTTCAAGG TCTGCCGGGA CAACAGCATC 
CTCCCACCTT TGGATAAAGA GAAAGGTGAA ACCTTGCTAA GCCCCTTAGT GATGTGCGGG 
CCCCATGGCC TCAAGTTCCT GAAGCCCGTG GAGCTGCGCT TGCCACACTG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTACC TTGTGGGAGC AAACTGTGTG 
TCTGTCTTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008268004.1
CDS	451..5454
Translation	MSARVAAAKNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGLANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSSRSRSPDQRSEPSDHSRQSPQQPSNGSLRSREEERISKPGAVSTPVKHGDDHTPKTVEEITVERNEKQTPALPEPKPVYAQVGQPDVDLPVSPSDGVLPNSAHEDGMLRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVPRIDSPGFKTASQQVYRKDPYPEEVMRQNPALKQPAVGHPGQRPEKEPSLTYEPQLPYMEKQASRDLEQPTYRYESSGYTDPFSRNYDHRLRYEDRMPAYEEQWSYYDDKQPYQPRPPFDSQHPRDLDPRQHPDEPSERGYFPRFEEPAPLSYDSRPRYEQPPRTSTLRHEEQPAPGYDVHNRQRPEAQPYPAAAPKSAEPKQYFDQYGRSYEQIPPQGFASRTGHYEPLHSAAVVPPLIPSSQHKPEVLPSSTKPLPPPPALTEEDEDPAMKPQSVLTRVKMFENKRSASLENKKEVNDTASFKPPEASKLPGAPVTGPKSTPQSQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPAAHIPASHLSEPAKPAHSQNQPNFSSYSSKGKAEADALDRSFGEKRYEPVQPTPPPPPLPSQYSQAAQPGASSSLHMHSKGIHGEGNSMSLDFQNSLVSKPDPPPSQSKPAAFRAPHRDDAAQPALYPQKSFPEKAPVNGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPGDTVHKPDLSAKAPASPKTLMKAHSSTQPPEFDSGVETFSIHAEKPKYQVTNISTVPRAAPVGPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_008269782.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008269782.1

ORF Insert Sequence:

ATGTCCGCCA GAGTGGCGGC CGCCAAGAAC ACGGCCATGG AGGAAACAGC TATATGGGAA 
CAACACACAG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCGGGT 
GGAAGAGATA ACCCTCATTT TCAGAGTGGA GAGACCTCCA TAGTAATTTC AGATGTGCTG 
AAGGGAGGAC CAGCCGAGGG GCAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGG 
GTTTCCATGG ATAACGTTGA ACATGCCTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAGA TTACCATTCG ACGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TGTCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATCCA TGACCCGAGA 
AGTGGCCGCA GTGGTCTGGC AAACAGAAGG AGTGAGAAGA GTTGGGCAAG GGATAGAAGT 
GCGAGTCGAG AGAGGAGCTT GTCTCCGCGA TCAGATAGGC GGTCGGTGGC CTCTAGCCAG 
CCTGCGAAAC CCACCAAAGT CACTTTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCACATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAACATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACGGTGAC AGAAAATATG 
TCATTGACTG ATGCGAAGAC GTTGATAGAA AGGTCTAAAG GCAAGTTGAA AATGGTAGTC 
CAGAGAGATG AACGGGCGAC CTTGCTGAAC GTCCCTGACC TTTCTGACAG CATCCATTCT 
GCTAATGCCT CCGAGAGAGA TGACATTTCA GAAATCCAGT CACTGGCATC AGATCATTCT 
GGCCGGTCAC ATGACAGGCC TCCACGCCGC AGCAGCCGGT CCCGCTCTCC TGACCAGCGG 
TCTGAGCCTT CCGACCACTC CAGACAGTCT CCGCAGCAGC CCAGCAATGG CAGTCTCCGG 
AGCAGAGAAG AAGAGAGAAT TTCTAAGCCT GGGGCTGTCT CGACTCCTGT AAAGCATGGA 
GATGACCACA CACCTAAAAC AGTGGAAGAA ATTACTGTTG AGAGAAATGA GAAGCAAACA 
CCTGCCCTTC CAGAACCAAA GCCTGTGTAC GCTCAAGTAG GACAACCAGA CGTGGATTTA 
CCTGTCAGCC CATCTGATGG TGTCCTGCCT AACTCAGCGC ACGAGGACGG GATGCTTCGG 
CCCAGCATGA AATTGGTAAA ATTCAGAAAA GGAGATAGCG TGGGTCTGCG GCTGGCTGGT 
GGAAATGATG TCGGAATATT TGTGGCTGGC GTTCTGGAAG ATAGCCCTGC TGCTAAAGAA 
GGCTTAGAGG AGGGTGATCA GATTCTCAGG GTAAACAATG TGGATTTCAC CAATATCATT 
AGAGAAGAAG CTGTCCTTTT CCTGCTTGAC CTCCCTAAAG GAGAAGAAGT GACCATCCTG 
GCCCAGAAGA AGAAGGATGT TTATCGTCGC ATTGTAGAAT CAGATGTTGG AGATTCTTTC 
TATATTAGAA CCCATTTTGA ATACGAAAAG GAATCTCCCT ATGGACTTAG TTTTAACAAG 
GGAGAGGTAT TTCGTGTTGT GGATACCTTG TACAATGGAA AACTGGGCTC TTGGCTTGCT 
ATTCGAATTG GCAAAAATCA TAAGGAGGTG GAACGAGGCA TCATCCCTAA TAAGAACAGA 
GCTGAACAGC TGGCCAGTGT GCAGTATACA CTTCCCAAAA CTGCGGGGGG CGATCGTGCG 
GACTTCTGGA GGTTCCGGGG TCTCCGCAGC TCCAAGAGGA ATCTTCGGAA AAGCAGAGAG 
GATTTGTCAG CCCAGCCAGT TCAGACGAAA TTTCCAGCTT ATGAAAGGGT TGTTCTTCGA 
GAAGCTGGAT TCCTGAGGCC TGTAACCATC TTTGGACCCA TAGCCGATGT TGCCAGAGAA 
AAGCTGGCCA GAGAAGAGCC AGATATTTAT CAGATTGCAA AAAGTGAACC GCGAGATGCT 
GGAACTGACC AACGTAGCTC TGGCATTATT CGACTTCATA CAATAAAGCA AATAATAGAT 
CAAGACAAGC ATGCCTTATT GGACGTAACA CCAAATGCAG TAGATCGTCT TAATTATGCC 
CAGTGGTATC CAATTGTTGT TTTCCTTAAC CCTGATTCTA AGCAAGGTGT GAAAACAATG 
AGAATGAGGT TATGTCCAGA ATCTCGGAAA AGTGCCAGGA AGTTATATGA GCGCTCTCAC 
AAACTCCGTA AAAACAATCA CCATCTTTTC ACAACTACAA TTAACTTAAA TTCAATGAAC 
GATGGCTGGT ATGGTGCGTT GAAGGAAGCA ATCCAGCAAC AGCAGAACCA GCTGGTATGG 
GTTTCTGAGG GAAAGGCGGA TGGTGCTACA AGTGATGACC TTGATTTGCA TGATGATCGT 
CTGTCCTACC TGTCAGCCCC AGGTAGTGAA TACTCAATGT ATAGCACGGA CAGTAGACAC 
ACTTCTGACT ATGAAGACAC AGATACAGAA GGCGGGGCCT ACACTGATCA AGAACTAGAT 
GAAACTCTTA ATGATGAGGT TGGGACTCCA CCGGAGTCTG CCATTACACG GTCCTCTGAG 
CCTGTAAGAG AGGACTCCTC TGGGATGCAT CATGAAAACC AAACATATCC TCCCTACTCA 
CCACAAGCGC AGCCACAGCC AGTTCCTAGA ATAGACTCCC CGGGATTTAA GACAGCCTCT 
CAACAGGTCT ATAGAAAGGA TCCGTATCCT GAGGAAGTGA TGAGACAGAA TCCAGCTTTG 
AAACAGCCAG CTGTTGGTCA CCCAGGGCAG AGGCCTGAGA AAGAGCCCAG TCTGACCTAT 
GAGCCCCAGC TTCCCTACAT GGAGAAACAA GCCAGCAGAG ACCTCGAGCA GCCCACATAC 
AGATACGAGT CCTCCGGCTA CACAGACCCG TTTTCTCGAA ACTATGACCA TCGCCTGCGA 
TACGAGGACC GCATGCCTGC ATATGAAGAA CAGTGGTCGT ACTACGATGA CAAACAGCCC 
TACCAGCCTC GGCCGCCTTT CGATAGTCAG CACCCTCGAG ACCTTGACCC TAGACAGCAC 
CCTGACGAGC CCTCAGAACG AGGCTATTTC CCACGTTTTG AAGAGCCAGC CCCCCTGTCT 
TATGACAGTC GGCCACGCTA CGAACAGCCG CCCAGAACCT CCACCCTGCG GCATGAAGAG 
CAGCCAGCTC CGGGCTATGA TGTGCACAAC AGACAGAGAC CAGAAGCGCA GCCCTATCCT 
GCAGCAGCCC CTAAGTCGGC GGAGCCCAAG CAGTACTTTG ACCAGTACGG GCGAAGCTAC 
GAGCAAATCC CGCCTCAAGG GTTTGCCTCC AGAACAGGCC ACTACGAACC TCTCCACAGC 
GCCGCAGTCG TCCCTCCGCT CATACCTTCC TCTCAGCATA AGCCAGAAGT CCTGCCTTCC 
AGCACCAAGC CACTGCCCCC ACCTCCAGCC CTAACCGAGG AGGACGAGGA CCCAGCGATG 
AAACCACAGT CTGTGCTCAC CCGAGTGAAA ATGTTTGAAA ACAAAAGGTC TGCATCCCTG 
GAGAACAAGA AGGAGGTGAA TGACACGGCC AGTTTTAAGC CTCCAGAAGC ATCCAAACTT 
CCAGGTGCTC CCGTCACTGG GCCTAAATCC ACCCCTCAGA GCCAGTTCAG TGAACATGAC 
AAAACTTTGT ACAGGATCCC AGAGCCTCAA AAACCGCAAA TGAAGCCTCC TGAAGATATT 
GTCCGGTCAA ATCATTACGA TCCGGAAGAA GATGAAGAGT ATTACCGAAA GCAGCTCTCG 
TACTTTGACC GGAGAAGTTT CGAGAACAAG CCTGCGGCAC ACATTCCTGC CAGCCATCTC 
TCAGAGCCTG CCAAGCCGGC TCATTCTCAG AACCAACCAA ATTTTTCTAG TTATTCTTCA 
AAGGGGAAAG CTGAAGCTGA TGCTCTGGAC CGATCGTTTG GTGAGAAGCG CTATGAGCCA 
GTGCAGCCCA CCCCTCCCCC TCCCCCACTG CCCTCGCAGT ACTCGCAGGC GGCCCAGCCT 
GGCGCCAGCT CTTCTCTCCA CATGCACTCG AAGGGGATAC ATGGTGAAGG TAATTCAATG 
TCATTGGATT TTCAGAATTC TTTGGTATCC AAACCAGACC CACCTCCATC TCAGAGTAAG 
CCAGCAGCTT TCAGAGCACC GCACCGAGAC GATGCCGCTC AGCCTGCTTT ATATCCCCAG 
AAAAGCTTCC CGGAGAAAGC CCCCGTTAAT GGAGCTGAAC AGACTCAGAA AACCATCACT 
CCAGCCTACA ACCGATTCAC ACCAAAACCA TATACCAGTT CTGCCCGGCC ATTCGAGCGC 
AAATTTGAAA GTCCTAAGTT CAACCACAAT CTTCTGCCAG GTGACACTGT ACATAAACCT 
GACTTGTCTG CAAAAGCCCC AGCTTCTCCA AAAACTCTTA TGAAAGCGCA CAGTTCCACA 
CAGCCTCCTG AGTTTGACAG TGGAGTGGAA ACTTTCTCCA TCCATGCAGA GAAGCCCAAG 
TACCAAGTAA CTAATATCAG CACAGTGCCT AGAGCTGCTC CTGTGGGCCC ATCCGCTGTG 
GAAGAAGATG AAGACGAGGA TGGGCACACT GTGGTGGCCA CAGCCCGAGG CATATTTAAC 
AGCAATGGTG GGGTTTTGAG CTCCATAGAG ACCGGCGTCA GTATAATTAT CCCGCAAGGA 
GCCATTCCCG AAGGAATTGA GCAGGAAATC TATTTCAAGG TCTGCCGGGA CAACAGCATC 
CTCCCACCTT TGGATAAAGA GAAAGGTGAA ACCTTGCTAA GCCCCTTAGT GATGTGCGGG 
CCCCATGGCC TCAAGTTCCT GAAGCCCGTG GAGCTGCGCT TGCCACACTG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTACC TTGTGGGAGC AAACTGTGTG 
TCTGTCTTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017348357.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_017203846.1 Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOb37767
Clone ID Related Accession (Same CDS sequence)	XM_017348357.1
Accession Version	XM_017348357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013685.1) and transcript sequence (GBCI01037002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 477 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCCGCCA GAGTGGCGGC CGCCAAGAAC ACGGCCATGG AGGAAACAGC TATATGGGAA 
CAACACACAG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCGGGT 
GGAAGAGATA ACCCTCATTT TCAGAGTGGA GAGACCTCCA TAGTAATTTC AGATGTGCTG 
AAGGGAGGAC CAGCCGAGGG GCAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGG 
GTTTCCATGG ATAACGTTGA ACATGCCTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAGA TTACCATTCG ACGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TGTCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATCCA TGACCCGAGA 
AGTGGCCGCA GTGGTCTGGC AAACAGAAGG AGTGAGAAGA GTTGGGCAAG GGATAGAAGT 
GCGAGTCGAG AGAGGAGCTT GTCTCCGCGA TCAGATAGGC GGTCGGTGGC CTCTAGCCAG 
CCTGCGAAAC CCACCAAAGT CACTTTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCACATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAACATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACGGTGAC AGAAAATATG 
TCATTGACTG ATGCGAAGAC GTTGATAGAA AGGTCTAAAG GCAAGTTGAA AATGGTAGTC 
CAGAGAGATG AACGGGCGAC CTTGCTGAAC GTCCCTGACC TTTCTGACAG CATCCATTCT 
GCTAATGCCT CCGAGAGAGA TGACATTTCA GAAATCCAGT CACTGGCATC AGATCATTCT 
GGCCGGTCAC ATGACAGGCC TCCACGCCGC AGCAGCCGGT CCCGCTCTCC TGACCAGCGG 
TCTGAGCCTT CCGACCACTC CAGACAGTCT CCGCAGCAGC CCAGCAATGG CAGTCTCCGG 
AGCAGAGAAG AAGAGAGAAT TTCTAAGCCT GGGGCTGTCT CGACTCCTGT AAAGCATGGA 
GATGACCACA CACCTAAAAC AGTGGAAGAA ATTACTGTTG AGAGAAATGA GAAGCAAACA 
CCTGCCCTTC CAGAACCAAA GCCTGTGTAC GCTCAAGTAG GACAACCAGA CGTGGATTTA 
CCTGTCAGCC CATCTGATGG TGTCCTGCCT AACTCAGCGC ACGAGGACGG GATGCTTCGG 
CCCAGCATGA AATTGGTAAA ATTCAGAAAA GGAGATAGCG TGGGTCTGCG GCTGGCTGGT 
GGAAATGATG TCGGAATATT TGTGGCTGGC GTTCTGGAAG ATAGCCCTGC TGCTAAAGAA 
GGCTTAGAGG AGGGTGATCA GATTCTCAGG GTAAACAATG TGGATTTCAC CAATATCATT 
AGAGAAGAAG CTGTCCTTTT CCTGCTTGAC CTCCCTAAAG GAGAAGAAGT GACCATCCTG 
GCCCAGAAGA AGAAGGATGT TTATCGTCGC ATTGTAGAAT CAGATGTTGG AGATTCTTTC 
TATATTAGAA CCCATTTTGA ATACGAAAAG GAATCTCCCT ATGGACTTAG TTTTAACAAG 
GGAGAGGTAT TTCGTGTTGT GGATACCTTG TACAATGGAA AACTGGGCTC TTGGCTTGCT 
ATTCGAATTG GCAAAAATCA TAAGGAGGTG GAACGAGGCA TCATCCCTAA TAAGAACAGA 
GCTGAACAGC TGGCCAGTGT GCAGTATACA CTTCCCAAAA CTGCGGGGGG CGATCGTGCG 
GACTTCTGGA GGTTCCGGGG TCTCCGCAGC TCCAAGAGGA ATCTTCGGAA AAGCAGAGAG 
GATTTGTCAG CCCAGCCAGT TCAGACGAAA TTTCCAGCTT ATGAAAGGGT TGTTCTTCGA 
GAAGCTGGAT TCCTGAGGCC TGTAACCATC TTTGGACCCA TAGCCGATGT TGCCAGAGAA 
AAGCTGGCCA GAGAAGAGCC AGATATTTAT CAGATTGCAA AAAGTGAACC GCGAGATGCT 
GGAACTGACC AACGTAGCTC TGGCATTATT CGACTTCATA CAATAAAGCA AATAATAGAT 
CAAGACAAGC ATGCCTTATT GGACGTAACA CCAAATGCAG TAGATCGTCT TAATTATGCC 
CAGTGGTATC CAATTGTTGT TTTCCTTAAC CCTGATTCTA AGCAAGGTGT GAAAACAATG 
AGAATGAGGT TATGTCCAGA ATCTCGGAAA AGTGCCAGGA AGTTATATGA GCGCTCTCAC 
AAACTCCGTA AAAACAATCA CCATCTTTTC ACAACTACAA TTAACTTAAA TTCAATGAAC 
GATGGCTGGT ATGGTGCGTT GAAGGAAGCA ATCCAGCAAC AGCAGAACCA GCTGGTATGG 
GTTTCTGAGG GAAAGGCGGA TGGTGCTACA AGTGATGACC TTGATTTGCA TGATGATCGT 
CTGTCCTACC TGTCAGCCCC AGGTAGTGAA TACTCAATGT ATAGCACGGA CAGTAGACAC 
ACTTCTGACT ATGAAGACAC AGATACAGAA GGCGGGGCCT ACACTGATCA AGAACTAGAT 
GAAACTCTTA ATGATGAGGT TGGGACTCCA CCGGAGTCTG CCATTACACG GTCCTCTGAG 
CCTGTAAGAG AGGACTCCTC TGGGATGCAT CATGAAAACC AAACATATCC TCCCTACTCA 
CCACAAGCGC AGCCACAGCC AGTTCCTAGA ATAGACTCCC CGGGATTTAA GACAGCCTCT 
CAACAGAAAG CAGAAGCCTC ATCTCCAGTC CCTTACCTTT CGCCTGAAAC AAACCCAGCA 
TCATCAACCT CTGCTGTTAA TCATAATGTA AATTTAACTA ATGTCAGACT GGAGGAGCCC 
ACCCCAGCTC CTCCCACCTC TCACTCACCA CAAGCTGATT CTTTAAGAAC ACCCAGCACT 
GAGGCAGCTC ACATAATGCT AAGAGATCAA GACCCATCAT TGTCGCCGCA TGTAGATCCA 
GCAAAGGTCT ATAGAAAGGA TCCGTATCCT GAGGAAGTGA TGAGACAGAA TCCAGCTTTG 
AAACAGCCAG CTGTTGGTCA CCCAGGGCAG AGGCCTGAGA AAGAGCCCAG TCTGACCTAT 
GAGCCCCAGC TTCCCTACAT GGAGAAACAA GCCAGCAGAG ACCTCGAGCA GCCCACATAC 
AGATACGAGT CCTCCGGCTA CACAGACCCG TTTTCTCGAA ACTATGACCA TCGCCTGCGA 
TACGAGGACC GCATGCCTGC ATATGAAGAA CAGTGGTCGT ACTACGATGA CAAACAGCCC 
TACCAGCCTC GGCCGCCTTT CGATAGTCAG CACCCTCGAG ACCTTGACCC TAGACAGCAC 
CCTGACGAGC CCTCAGAACG AGGCTATTTC CCACGTTTTG AAGAGCCAGC CCCCCTGTCT 
TATGACAGTC GGCCACGCTA CGAACAGCCG CCCAGAACCT CCACCCTGCG GCATGAAGAG 
CAGCCAGCTC CGGGCTATGA TGTGCACAAC AGACAGAGAC CAGAAGCGCA GCCCTATCCT 
GCAGCAGCCC CTAAGTCGGC GGAGCCCAAG CAGTACTTTG ACCAGTACGG GCGAAGCTAC 
GAGCAAATCC CGCCTCAAGG GTTTGCCTCC AGAACAGGCC ACTACGAACC TCTCCACAGC 
GCCGCAGTCG TCCCTCCGCT CATACCTTCC TCTCAGCATA AGCCAGAAGT CCTGCCTTCC 
AGCACCAAGC CACTGCCCCC ACCTCCAGCC CTAACCGAGG AGGACGAGGA CCCAGCGATG 
AAACCACAGT CTGTGCTCAC CCGAGTGAAA ATGTTTGAAA ACAAAAGGTC TGCATCCCTG 
GAGAACAAGA AGGAGGTGAA TGACACGGCC AGTTTTAAGC CTCCAGAAGC ATCCAAACTT 
CCAGGTGCTC CCGTCACTGG GCCTAAATCC ACCCCTCAGA GCCAGTTCAG TGAACATGAC 
AAAACTTTGT ACAGGATCCC AGAGCCTCAA AAACCGCAAA TGAAGCCTCC TGAAGATATT 
GTCCGGTCAA ATCATTACGA TCCGGAAGAA GATGAAGAGT ATTACCGAAA GCAGCTCTCG 
TACTTTGACC GGAGAAGTTT CGAGAACAAG CCTGCGGCAC ACATTCCTGC CAGCCATCTC 
TCAGAGCCTG CCAAGCCGGC TCATTCTCAG AACCAACCAA ATTTTTCTAG TTATTCTTCA 
AAGGGGAAAG CTGAAGCTGA TGCTCTGGAC CGATCGTTTG GTGAGAAGCG CTATGAGCCA 
GTGCAGCCCA CCCCTCCCCC TCCCCCACTG CCCTCGCAGT ACTCGCAGGC GGCCCAGCCT 
GGCGCCAGCT CTTCTCTCCA CATGCACTCG AAGGGGATAC ATGGTGAAGG TAATTCAATG 
TCATTGGATT TTCAGAATTC TTTGGTATCC AAACCAGACC CACCTCCATC TCAGAGTAAG 
CCAGCAGCTT TCAGAGCACC GCACCGAGAC GATGCCGCTC AGCCTGCTTT ATATCCCCAG 
AAAAGCTTCC CGGAGAAAGC CCCCGTTAAT GGAGCTGAAC AGACTCAGAA AACCATCACT 
CCAGCCTACA ACCGATTCAC ACCAAAACCA TATACCAGTT CTGCCCGGCC ATTCGAGCGC 
AAATTTGAAA GTCCTAAGTT CAACCACAAT CTTCTGCCAG GTGACACTGT ACATAAACCT 
GACTTGTCTG CAAAAGCCCC AGCTTCTCCA AAAACTCTTA TGAAAGCGCA CAGTTCCACA 
CAGCCTCCTG AGTTTGACAG TGGAGTGGAA ACTTTCTCCA TCCATGCAGA GAAGCCCAAG 
TACCAAGTAA CTAATATCAG CACAGTGCCT AGAGCTGCTC CTGTGGGCCC ATCCGCTGTG 
GAAGAAGATG AAGACGAGGA TGGGCACACT GTGGTGGCCA CAGCCCGAGG CATATTTAAC 
AGCAATGGTG GGGTTTTGAG CTCCATAGAG ACCGGCGTCA GTATAATTAT CCCGCAAGGA 
GCCATTCCCG AAGGAATTGA GCAGGAAATC TATTTCAAGG TCTGCCGGGA CAACAGCATC 
CTCCCACCTT TGGATAAAGA GAAAGGTGAA ACCTTGCTAA GCCCCTTAGT GATGTGCGGG 
CCCCATGGCC TCAAGTTCCT GAAGCCCGTG GAGCTGCGCT TGCCACACTG TGCGTCCATG 
ACTCCTGACG GTTGGTCTTT TGCTCTAAAA TCATCCGACT CCTCGTCGGG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTACC TTGTGGGAGC AAACTGTGTG 
TCTGTCTTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017203846.1
CDS	451..5754
Translation	MSARVAAAKNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGLANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSSRSRSPDQRSEPSDHSRQSPQQPSNGSLRSREEERISKPGAVSTPVKHGDDHTPKTVEEITVERNEKQTPALPEPKPVYAQVGQPDVDLPVSPSDGVLPNSAHEDGMLRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVPRIDSPGFKTASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPPTSHSPQADSLRTPSTEAAHIMLRDQDPSLSPHVDPAKVYRKDPYPEEVMRQNPALKQPAVGHPGQRPEKEPSLTYEPQLPYMEKQASRDLEQPTYRYESSGYTDPFSRNYDHRLRYEDRMPAYEEQWSYYDDKQPYQPRPPFDSQHPRDLDPRQHPDEPSERGYFPRFEEPAPLSYDSRPRYEQPPRTSTLRHEEQPAPGYDVHNRQRPEAQPYPAAAPKSAEPKQYFDQYGRSYEQIPPQGFASRTGHYEPLHSAAVVPPLIPSSQHKPEVLPSSTKPLPPPPALTEEDEDPAMKPQSVLTRVKMFENKRSASLENKKEVNDTASFKPPEASKLPGAPVTGPKSTPQSQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPAAHIPASHLSEPAKPAHSQNQPNFSSYSSKGKAEADALDRSFGEKRYEPVQPTPPPPPLPSQYSQAAQPGASSSLHMHSKGIHGEGNSMSLDFQNSLVSKPDPPPSQSKPAAFRAPHRDDAAQPALYPQKSFPEKAPVNGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPGDTVHKPDLSAKAPASPKTLMKAHSSTQPPEFDSGVETFSIHAEKPKYQVTNISTVPRAAPVGPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017348357.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017348357.1

ORF Insert Sequence:

ATGTCCGCCA GAGTGGCGGC CGCCAAGAAC ACGGCCATGG AGGAAACAGC TATATGGGAA 
CAACACACAG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCGGGT 
GGAAGAGATA ACCCTCATTT TCAGAGTGGA GAGACCTCCA TAGTAATTTC AGATGTGCTG 
AAGGGAGGAC CAGCCGAGGG GCAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGG 
GTTTCCATGG ATAACGTTGA ACATGCCTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAGA TTACCATTCG ACGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TGTCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATCCA TGACCCGAGA 
AGTGGCCGCA GTGGTCTGGC AAACAGAAGG AGTGAGAAGA GTTGGGCAAG GGATAGAAGT 
GCGAGTCGAG AGAGGAGCTT GTCTCCGCGA TCAGATAGGC GGTCGGTGGC CTCTAGCCAG 
CCTGCGAAAC CCACCAAAGT CACTTTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCACATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAACATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACGGTGAC AGAAAATATG 
TCATTGACTG ATGCGAAGAC GTTGATAGAA AGGTCTAAAG GCAAGTTGAA AATGGTAGTC 
CAGAGAGATG AACGGGCGAC CTTGCTGAAC GTCCCTGACC TTTCTGACAG CATCCATTCT 
GCTAATGCCT CCGAGAGAGA TGACATTTCA GAAATCCAGT CACTGGCATC AGATCATTCT 
GGCCGGTCAC ATGACAGGCC TCCACGCCGC AGCAGCCGGT CCCGCTCTCC TGACCAGCGG 
TCTGAGCCTT CCGACCACTC CAGACAGTCT CCGCAGCAGC CCAGCAATGG CAGTCTCCGG 
AGCAGAGAAG AAGAGAGAAT TTCTAAGCCT GGGGCTGTCT CGACTCCTGT AAAGCATGGA 
GATGACCACA CACCTAAAAC AGTGGAAGAA ATTACTGTTG AGAGAAATGA GAAGCAAACA 
CCTGCCCTTC CAGAACCAAA GCCTGTGTAC GCTCAAGTAG GACAACCAGA CGTGGATTTA 
CCTGTCAGCC CATCTGATGG TGTCCTGCCT AACTCAGCGC ACGAGGACGG GATGCTTCGG 
CCCAGCATGA AATTGGTAAA ATTCAGAAAA GGAGATAGCG TGGGTCTGCG GCTGGCTGGT 
GGAAATGATG TCGGAATATT TGTGGCTGGC GTTCTGGAAG ATAGCCCTGC TGCTAAAGAA 
GGCTTAGAGG AGGGTGATCA GATTCTCAGG GTAAACAATG TGGATTTCAC CAATATCATT 
AGAGAAGAAG CTGTCCTTTT CCTGCTTGAC CTCCCTAAAG GAGAAGAAGT GACCATCCTG 
GCCCAGAAGA AGAAGGATGT TTATCGTCGC ATTGTAGAAT CAGATGTTGG AGATTCTTTC 
TATATTAGAA CCCATTTTGA ATACGAAAAG GAATCTCCCT ATGGACTTAG TTTTAACAAG 
GGAGAGGTAT TTCGTGTTGT GGATACCTTG TACAATGGAA AACTGGGCTC TTGGCTTGCT 
ATTCGAATTG GCAAAAATCA TAAGGAGGTG GAACGAGGCA TCATCCCTAA TAAGAACAGA 
GCTGAACAGC TGGCCAGTGT GCAGTATACA CTTCCCAAAA CTGCGGGGGG CGATCGTGCG 
GACTTCTGGA GGTTCCGGGG TCTCCGCAGC TCCAAGAGGA ATCTTCGGAA AAGCAGAGAG 
GATTTGTCAG CCCAGCCAGT TCAGACGAAA TTTCCAGCTT ATGAAAGGGT TGTTCTTCGA 
GAAGCTGGAT TCCTGAGGCC TGTAACCATC TTTGGACCCA TAGCCGATGT TGCCAGAGAA 
AAGCTGGCCA GAGAAGAGCC AGATATTTAT CAGATTGCAA AAAGTGAACC GCGAGATGCT 
GGAACTGACC AACGTAGCTC TGGCATTATT CGACTTCATA CAATAAAGCA AATAATAGAT 
CAAGACAAGC ATGCCTTATT GGACGTAACA CCAAATGCAG TAGATCGTCT TAATTATGCC 
CAGTGGTATC CAATTGTTGT TTTCCTTAAC CCTGATTCTA AGCAAGGTGT GAAAACAATG 
AGAATGAGGT TATGTCCAGA ATCTCGGAAA AGTGCCAGGA AGTTATATGA GCGCTCTCAC 
AAACTCCGTA AAAACAATCA CCATCTTTTC ACAACTACAA TTAACTTAAA TTCAATGAAC 
GATGGCTGGT ATGGTGCGTT GAAGGAAGCA ATCCAGCAAC AGCAGAACCA GCTGGTATGG 
GTTTCTGAGG GAAAGGCGGA TGGTGCTACA AGTGATGACC TTGATTTGCA TGATGATCGT 
CTGTCCTACC TGTCAGCCCC AGGTAGTGAA TACTCAATGT ATAGCACGGA CAGTAGACAC 
ACTTCTGACT ATGAAGACAC AGATACAGAA GGCGGGGCCT ACACTGATCA AGAACTAGAT 
GAAACTCTTA ATGATGAGGT TGGGACTCCA CCGGAGTCTG CCATTACACG GTCCTCTGAG 
CCTGTAAGAG AGGACTCCTC TGGGATGCAT CATGAAAACC AAACATATCC TCCCTACTCA 
CCACAAGCGC AGCCACAGCC AGTTCCTAGA ATAGACTCCC CGGGATTTAA GACAGCCTCT 
CAACAGAAAG CAGAAGCCTC ATCTCCAGTC CCTTACCTTT CGCCTGAAAC AAACCCAGCA 
TCATCAACCT CTGCTGTTAA TCATAATGTA AATTTAACTA ATGTCAGACT GGAGGAGCCC 
ACCCCAGCTC CTCCCACCTC TCACTCACCA CAAGCTGATT CTTTAAGAAC ACCCAGCACT 
GAGGCAGCTC ACATAATGCT AAGAGATCAA GACCCATCAT TGTCGCCGCA TGTAGATCCA 
GCAAAGGTCT ATAGAAAGGA TCCGTATCCT GAGGAAGTGA TGAGACAGAA TCCAGCTTTG 
AAACAGCCAG CTGTTGGTCA CCCAGGGCAG AGGCCTGAGA AAGAGCCCAG TCTGACCTAT 
GAGCCCCAGC TTCCCTACAT GGAGAAACAA GCCAGCAGAG ACCTCGAGCA GCCCACATAC 
AGATACGAGT CCTCCGGCTA CACAGACCCG TTTTCTCGAA ACTATGACCA TCGCCTGCGA 
TACGAGGACC GCATGCCTGC ATATGAAGAA CAGTGGTCGT ACTACGATGA CAAACAGCCC 
TACCAGCCTC GGCCGCCTTT CGATAGTCAG CACCCTCGAG ACCTTGACCC TAGACAGCAC 
CCTGACGAGC CCTCAGAACG AGGCTATTTC CCACGTTTTG AAGAGCCAGC CCCCCTGTCT 
TATGACAGTC GGCCACGCTA CGAACAGCCG CCCAGAACCT CCACCCTGCG GCATGAAGAG 
CAGCCAGCTC CGGGCTATGA TGTGCACAAC AGACAGAGAC CAGAAGCGCA GCCCTATCCT 
GCAGCAGCCC CTAAGTCGGC GGAGCCCAAG CAGTACTTTG ACCAGTACGG GCGAAGCTAC 
GAGCAAATCC CGCCTCAAGG GTTTGCCTCC AGAACAGGCC ACTACGAACC TCTCCACAGC 
GCCGCAGTCG TCCCTCCGCT CATACCTTCC TCTCAGCATA AGCCAGAAGT CCTGCCTTCC 
AGCACCAAGC CACTGCCCCC ACCTCCAGCC CTAACCGAGG AGGACGAGGA CCCAGCGATG 
AAACCACAGT CTGTGCTCAC CCGAGTGAAA ATGTTTGAAA ACAAAAGGTC TGCATCCCTG 
GAGAACAAGA AGGAGGTGAA TGACACGGCC AGTTTTAAGC CTCCAGAAGC ATCCAAACTT 
CCAGGTGCTC CCGTCACTGG GCCTAAATCC ACCCCTCAGA GCCAGTTCAG TGAACATGAC 
AAAACTTTGT ACAGGATCCC AGAGCCTCAA AAACCGCAAA TGAAGCCTCC TGAAGATATT 
GTCCGGTCAA ATCATTACGA TCCGGAAGAA GATGAAGAGT ATTACCGAAA GCAGCTCTCG 
TACTTTGACC GGAGAAGTTT CGAGAACAAG CCTGCGGCAC ACATTCCTGC CAGCCATCTC 
TCAGAGCCTG CCAAGCCGGC TCATTCTCAG AACCAACCAA ATTTTTCTAG TTATTCTTCA 
AAGGGGAAAG CTGAAGCTGA TGCTCTGGAC CGATCGTTTG GTGAGAAGCG CTATGAGCCA 
GTGCAGCCCA CCCCTCCCCC TCCCCCACTG CCCTCGCAGT ACTCGCAGGC GGCCCAGCCT 
GGCGCCAGCT CTTCTCTCCA CATGCACTCG AAGGGGATAC ATGGTGAAGG TAATTCAATG 
TCATTGGATT TTCAGAATTC TTTGGTATCC AAACCAGACC CACCTCCATC TCAGAGTAAG 
CCAGCAGCTT TCAGAGCACC GCACCGAGAC GATGCCGCTC AGCCTGCTTT ATATCCCCAG 
AAAAGCTTCC CGGAGAAAGC CCCCGTTAAT GGAGCTGAAC AGACTCAGAA AACCATCACT 
CCAGCCTACA ACCGATTCAC ACCAAAACCA TATACCAGTT CTGCCCGGCC ATTCGAGCGC 
AAATTTGAAA GTCCTAAGTT CAACCACAAT CTTCTGCCAG GTGACACTGT ACATAAACCT 
GACTTGTCTG CAAAAGCCCC AGCTTCTCCA AAAACTCTTA TGAAAGCGCA CAGTTCCACA 
CAGCCTCCTG AGTTTGACAG TGGAGTGGAA ACTTTCTCCA TCCATGCAGA GAAGCCCAAG 
TACCAAGTAA CTAATATCAG CACAGTGCCT AGAGCTGCTC CTGTGGGCCC ATCCGCTGTG 
GAAGAAGATG AAGACGAGGA TGGGCACACT GTGGTGGCCA CAGCCCGAGG CATATTTAAC 
AGCAATGGTG GGGTTTTGAG CTCCATAGAG ACCGGCGTCA GTATAATTAT CCCGCAAGGA 
GCCATTCCCG AAGGAATTGA GCAGGAAATC TATTTCAAGG TCTGCCGGGA CAACAGCATC 
CTCCCACCTT TGGATAAAGA GAAAGGTGAA ACCTTGCTAA GCCCCTTAGT GATGTGCGGG 
CCCCATGGCC TCAAGTTCCT GAAGCCCGTG GAGCTGCGCT TGCCACACTG TGCGTCCATG 
ACTCCTGACG GTTGGTCTTT TGCTCTAAAA TCATCCGACT CCTCGTCGGG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTACC TTGTGGGAGC AAACTGTGTG 
TCTGTCTTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017348358.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_017203847.1 Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X2, mRNA.

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CloneID	OOb37768
Clone ID Related Accession (Same CDS sequence)	XM_017348358.1
Accession Version	XM_017348358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013685.1) and transcript sequence (GBCI01037002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 477 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCCGCCA GAGTGGCGGC CGCCAAGAAC ACGGCCATGG AGGAAACAGC TATATGGGAA 
CAACACACAG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCGGGT 
GGAAGAGATA ACCCTCATTT TCAGAGTGGA GAGACCTCCA TAGTAATTTC AGATGTGCTG 
AAGGGAGGAC CAGCCGAGGG GCAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGG 
GTTTCCATGG ATAACGTTGA ACATGCCTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAGA TTACCATTCG ACGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TGTCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATCCA TGACCCGAGA 
AGTGGCCGCA GTGGTCTGGC AAACAGAAGG AGTGAGAAGA GTTGGGCAAG GGATAGAAGT 
GCGAGTCGAG AGAGGAGCTT GTCTCCGCGA TCAGATAGGC GGTCGGTGGC CTCTAGCCAG 
CCTGCGAAAC CCACCAAAGT CACTTTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCACATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAACATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACGGTGAC AGAAAATATG 
TCATTGACTG ATGCGAAGAC GTTGATAGAA AGGTCTAAAG GCAAGTTGAA AATGGTAGTC 
CAGAGAGATG AACGGGCGAC CTTGCTGAAC GTCCCTGACC TTTCTGACAG CATCCATTCT 
GCTAATGCCT CCGAGAGAGA TGACATTTCA GAAATCCAGT CACTGGCATC AGATCATTCT 
GGCCGGTCAC ATGACAGGCC TCCACGCCGC AGCAGCCGGT CCCGCTCTCC TGACCAGCGG 
TCTGAGCCTT CCGACCACTC CAGACAGTCT CCGCAGCAGC CCAGCAATGG CAGTCTCCGG 
AGCAGAGAAG AAGAGAGAAT TTCTAAGCCT GGGGCTGTCT CGACTCCTGT AAAGCATGGA 
GATGACCACA CACCTAAAAC AGTGGAAGAA ATTACTGTTG AGAGAAATGA GAAGCAAACA 
CCTGCCCTTC CAGAACCAAA GCCTGTGTAC GCTCAAGTAG GACAACCAGA CGTGGATTTA 
CCTGTCAGCC CATCTGATGG TGTCCTGCCT AACTCAGCGC ACGAGGACGG GATGCTTCGG 
CCCAGCATGA AATTGGTAAA ATTCAGAAAA GGAGATAGCG TGGGTCTGCG GCTGGCTGGT 
GGAAATGATG TCGGAATATT TGTGGCTGGC GTTCTGGAAG ATAGCCCTGC TGCTAAAGAA 
GGCTTAGAGG AGGGTGATCA GATTCTCAGG GTAAACAATG TGGATTTCAC CAATATCATT 
AGAGAAGAAG CTGTCCTTTT CCTGCTTGAC CTCCCTAAAG GAGAAGAAGT GACCATCCTG 
GCCCAGAAGA AGAAGGATGT TTATCGTCGC ATTGTAGAAT CAGATGTTGG AGATTCTTTC 
TATATTAGAA CCCATTTTGA ATACGAAAAG GAATCTCCCT ATGGACTTAG TTTTAACAAG 
GGAGAGGTAT TTCGTGTTGT GGATACCTTG TACAATGGAA AACTGGGCTC TTGGCTTGCT 
ATTCGAATTG GCAAAAATCA TAAGGAGGTG GAACGAGGCA TCATCCCTAA TAAGAACAGA 
GCTGAACAGC TGGCCAGTGT GCAGTATACA CTTCCCAAAA CTGCGGGGGG CGATCGTGCG 
GACTTCTGGA GGTTCCGGGG TCTCCGCAGC TCCAAGAGGA ATCTTCGGAA AAGCAGAGAG 
GATTTGTCAG CCCAGCCAGT TCAGACGAAA TTTCCAGCTT ATGAAAGGGT TGTTCTTCGA 
GAAGCTGGAT TCCTGAGGCC TGTAACCATC TTTGGACCCA TAGCCGATGT TGCCAGAGAA 
AAGCTGGCCA GAGAAGAGCC AGATATTTAT CAGATTGCAA AAAGTGAACC GCGAGATGCT 
GGAACTGACC AACGTAGCTC TGGCATTATT CGACTTCATA CAATAAAGCA AATAATAGAT 
CAAGACAAGC ATGCCTTATT GGACGTAACA CCAAATGCAG TAGATCGTCT TAATTATGCC 
CAGTGGTATC CAATTGTTGT TTTCCTTAAC CCTGATTCTA AGCAAGGTGT GAAAACAATG 
AGAATGAGGT TATGTCCAGA ATCTCGGAAA AGTGCCAGGA AGTTATATGA GCGCTCTCAC 
AAACTCCGTA AAAACAATCA CCATCTTTTC ACAACTACAA TTAACTTAAA TTCAATGAAC 
GATGGCTGGT ATGGTGCGTT GAAGGAAGCA ATCCAGCAAC AGCAGAACCA GCTGGTATGG 
GTTTCTGAGG GAAAGGCGGA TGGTGCTACA AGTGATGACC TTGATTTGCA TGATGATCGT 
CTGTCCTACC TGTCAGCCCC AGGTAGTGAA TACTCAATGT ATAGCACGGA CAGTAGACAC 
ACTTCTGACT ATGAAGACAC AGATACAGAA GGCGGGGCCT ACACTGATCA AGAACTAGAT 
GAAACTCTTA ATGATGAGGT TGGGACTCCA CCGGAGTCTG CCATTACACG GTCCTCTGAG 
CCTGTAAGAG AGGACTCCTC TGGGATGCAT CATGAAAACC AAACATATCC TCCCTACTCA 
CCACAAGCGC AGCCACAGCC AGTTCCTAGA ATAGACTCCC CGGGATTTAA GACAGCCTCT 
CAACAGAAAG CAGAAGCCTC ATCTCCAGTC CCTTACCTTT CGCCTGAAAC AAACCCAGCA 
TCATCAACCT CTGCTGTTAA TCATAATGTA AATTTAACTA ATGTCAGACT GGAGGAGCCC 
ACCCCAGCTC CTCCCACCTC TCACTCACCA CAAGCTGATT CTTTAAGAAC ACCCAGCACT 
GAGGCAGCTC ACATAATGCT AAGAGATCAA GACCCATCAT TGTCGCCGCA TGTAGATCCA 
GCAAAGGTCT ATAGAAAGGA TCCGTATCCT GAGGAAGTGA TGAGACAGAA TCCAGCTTTG 
AAACAGCCAG CTGTTGGTCA CCCAGGGCAG AGGCCTGAGA AAGAGCCCAG TCTGACCTAT 
GAGCCCCAGC TTCCCTACAT GGAGAAACAA GCCAGCAGAG ACCTCGAGCA GCCCACATAC 
AGATACGAGT CCTCCGGCTA CACAGACCCG TTTTCTCGAA ACTATGACCA TCGCCTGCGA 
TACGAGGACC GCATGCCTGC ATATGAAGAA CAGTGGTCGT ACTACGATGA CAAACAGCCC 
TACCAGCCTC GGCCGCCTTT CGATAGTCAG CACCCTCGAG ACCTTGACCC TAGACAGCAC 
CCTGACGAGC CCTCAGAACG AGGCTATTTC CCACGTTTTG AAGAGCCAGC CCCCCTGTCT 
TATGACAGTC GGCCACGCTA CGAACAGCCG CCCAGAACCT CCACCCTGCG GCATGAAGAG 
CAGCCAGCTC CGGGCTATGA TGTGCACAAC AGACAGAGAC CAGAAGCGCA GCCCTATCCT 
GCAGCAGCCC CTAAGTCGGC GGAGCCCAAG CAGTACTTTG ACCAGTACGG GCGAAGCTAC 
GAGCAAATCC CGCCTCAAGG GTTTGCCTCC AGAACAGGCC ACTACGAACC TCTCCACAGC 
GCCGCAGTCG TCCCTCCGCT CATACCTTCC TCTCAGCATA AGCCAGAAGT CCTGCCTTCC 
AGCACCAAGC CACTGCCCCC ACCTCCAGCC CTAACCGAGG AGGACGAGGA CCCAGCGATG 
AAACCACAGT CTGTGCTCAC CCGAGTGAAA ATGTTTGAAA ACAAAAGGTC TGCATCCCTG 
GAGAACAAGA AGGAGGTGAA TGACACGGCC AGTTTTAAGC CTCCAGAAGC ATCCAAACTT 
CCAGGTGCTC CCGTCACTGG GCCTAAATCC ACCCCTCAGA GCCAGTTCAG TGAACATGAC 
AAAACTTTGT ACAGGATCCC AGAGCCTCAA AAACCGCAAA TGAAGCCTCC TGAAGATATT 
GTCCGGTCAA ATCATTACGA TCCGGAAGAA GATGAAGAGT ATTACCGAAA GCAGCTCTCG 
TACTTTGACC GGAGAAGTTT CGAGAACAAG CCTGCGGCAC ACATTCCTGC CAGCCATCTC 
TCAGAGCCTG CCAAGCCGGC TCATTCTCAG AACCAACCAA ATTTTTCTAG TTATTCTTCA 
AAGGGGAAAG CTGAAGCTGA TGCTCTGGAC CGATCGTTTG GTGAGAAGCG CTATGAGCCA 
GTGCAGCCCA CCCCTCCCCC TCCCCCACTG CCCTCGCAGT ACTCGCAGGC GGCCCAGCCT 
GGCGCCAGCT CTTCTCTCCA CATGCACTCG AAGGGGATAC ATGGTGAAGG TAATTCAATG 
TCATTGGATT TTCAGAATTC TTTGGTATCC AAACCAGACC CACCTCCATC TCAGAGTAAG 
CCAGCAGCTT TCAGAGCACC GCACCGAGAC GATGCCGCTC AGCCTGCTTT ATATCCCCAG 
AAAAGCTTCC CGGAGAAAGC CCCCGTTAAT GGAGCTGAAC AGACTCAGAA AACCATCACT 
CCAGCCTACA ACCGATTCAC ACCAAAACCA TATACCAGTT CTGCCCGGCC ATTCGAGCGC 
AAATTTGAAA GTCCTAAGTT CAACCACAAT CTTCTGCCAG GTGACACTGT ACATAAACCT 
GACTTGTCTG CAAAAGCCCC AGCTTCTCCA AAAACTCTTA TGAAAGCGCA CAGTTCCACA 
CAGCCTCCTG AGTTTGACAG TGGAGTGGAA ACTTTCTCCA TCCATGCAGA GAAGCCCAAG 
TACCAAGTAA CTAATATCAG CACAGTGCCT AGAGCTGCTC CTGTGGGCCC ATCCGCTGTG 
GAAGAAGATG AAGACGAGGA TGGGCACACT GTGGTGGCCA CAGCCCGAGG CATATTTAAC 
AGCAATGGTG GGGTTTTGAG CTCCATAGAG ACCGGCGTCA GTATAATTAT CCCGCAAGGA 
GCCATTCCCG AAGGAATTGA GCAGGAAATC TATTTCAAGG TCTGCCGGGA CAACAGCATC 
CTCCCACCTT TGGATAAAGA GAAAGGTGAA ACCTTGCTAA GCCCCTTAGT GATGTGCGGG 
CCCCATGGCC TCAAGTTCCT GAAGCCCGTG GAGCTGCGCT TGCCACACTG TGCGTCCATG 
ACTCCTGACG GTGATCCTAA AACCTGGCAA AACAAGTGTC TTCCTGGAGA TCCAAATTAC 
CTTGTGGGAG CAAACTGTGT GTCTGTCTTG ATTGACCACT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017203847.1
CDS	451..5715
Translation	MSARVAAAKNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGLANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSSRSRSPDQRSEPSDHSRQSPQQPSNGSLRSREEERISKPGAVSTPVKHGDDHTPKTVEEITVERNEKQTPALPEPKPVYAQVGQPDVDLPVSPSDGVLPNSAHEDGMLRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVPRIDSPGFKTASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPPTSHSPQADSLRTPSTEAAHIMLRDQDPSLSPHVDPAKVYRKDPYPEEVMRQNPALKQPAVGHPGQRPEKEPSLTYEPQLPYMEKQASRDLEQPTYRYESSGYTDPFSRNYDHRLRYEDRMPAYEEQWSYYDDKQPYQPRPPFDSQHPRDLDPRQHPDEPSERGYFPRFEEPAPLSYDSRPRYEQPPRTSTLRHEEQPAPGYDVHNRQRPEAQPYPAAAPKSAEPKQYFDQYGRSYEQIPPQGFASRTGHYEPLHSAAVVPPLIPSSQHKPEVLPSSTKPLPPPPALTEEDEDPAMKPQSVLTRVKMFENKRSASLENKKEVNDTASFKPPEASKLPGAPVTGPKSTPQSQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPAAHIPASHLSEPAKPAHSQNQPNFSSYSSKGKAEADALDRSFGEKRYEPVQPTPPPPPLPSQYSQAAQPGASSSLHMHSKGIHGEGNSMSLDFQNSLVSKPDPPPSQSKPAAFRAPHRDDAAQPALYPQKSFPEKAPVNGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPGDTVHKPDLSAKAPASPKTLMKAHSSTQPPEFDSGVETFSIHAEKPKYQVTNISTVPRAAPVGPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017348358.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017348358.1

ORF Insert Sequence:

ATGTCCGCCA GAGTGGCGGC CGCCAAGAAC ACGGCCATGG AGGAAACAGC TATATGGGAA 
CAACACACAG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCGGGT 
GGAAGAGATA ACCCTCATTT TCAGAGTGGA GAGACCTCCA TAGTAATTTC AGATGTGCTG 
AAGGGAGGAC CAGCCGAGGG GCAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGG 
GTTTCCATGG ATAACGTTGA ACATGCCTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAGA TTACCATTCG ACGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TGTCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATCCA TGACCCGAGA 
AGTGGCCGCA GTGGTCTGGC AAACAGAAGG AGTGAGAAGA GTTGGGCAAG GGATAGAAGT 
GCGAGTCGAG AGAGGAGCTT GTCTCCGCGA TCAGATAGGC GGTCGGTGGC CTCTAGCCAG 
CCTGCGAAAC CCACCAAAGT CACTTTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCACATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAACATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACGGTGAC AGAAAATATG 
TCATTGACTG ATGCGAAGAC GTTGATAGAA AGGTCTAAAG GCAAGTTGAA AATGGTAGTC 
CAGAGAGATG AACGGGCGAC CTTGCTGAAC GTCCCTGACC TTTCTGACAG CATCCATTCT 
GCTAATGCCT CCGAGAGAGA TGACATTTCA GAAATCCAGT CACTGGCATC AGATCATTCT 
GGCCGGTCAC ATGACAGGCC TCCACGCCGC AGCAGCCGGT CCCGCTCTCC TGACCAGCGG 
TCTGAGCCTT CCGACCACTC CAGACAGTCT CCGCAGCAGC CCAGCAATGG CAGTCTCCGG 
AGCAGAGAAG AAGAGAGAAT TTCTAAGCCT GGGGCTGTCT CGACTCCTGT AAAGCATGGA 
GATGACCACA CACCTAAAAC AGTGGAAGAA ATTACTGTTG AGAGAAATGA GAAGCAAACA 
CCTGCCCTTC CAGAACCAAA GCCTGTGTAC GCTCAAGTAG GACAACCAGA CGTGGATTTA 
CCTGTCAGCC CATCTGATGG TGTCCTGCCT AACTCAGCGC ACGAGGACGG GATGCTTCGG 
CCCAGCATGA AATTGGTAAA ATTCAGAAAA GGAGATAGCG TGGGTCTGCG GCTGGCTGGT 
GGAAATGATG TCGGAATATT TGTGGCTGGC GTTCTGGAAG ATAGCCCTGC TGCTAAAGAA 
GGCTTAGAGG AGGGTGATCA GATTCTCAGG GTAAACAATG TGGATTTCAC CAATATCATT 
AGAGAAGAAG CTGTCCTTTT CCTGCTTGAC CTCCCTAAAG GAGAAGAAGT GACCATCCTG 
GCCCAGAAGA AGAAGGATGT TTATCGTCGC ATTGTAGAAT CAGATGTTGG AGATTCTTTC 
TATATTAGAA CCCATTTTGA ATACGAAAAG GAATCTCCCT ATGGACTTAG TTTTAACAAG 
GGAGAGGTAT TTCGTGTTGT GGATACCTTG TACAATGGAA AACTGGGCTC TTGGCTTGCT 
ATTCGAATTG GCAAAAATCA TAAGGAGGTG GAACGAGGCA TCATCCCTAA TAAGAACAGA 
GCTGAACAGC TGGCCAGTGT GCAGTATACA CTTCCCAAAA CTGCGGGGGG CGATCGTGCG 
GACTTCTGGA GGTTCCGGGG TCTCCGCAGC TCCAAGAGGA ATCTTCGGAA AAGCAGAGAG 
GATTTGTCAG CCCAGCCAGT TCAGACGAAA TTTCCAGCTT ATGAAAGGGT TGTTCTTCGA 
GAAGCTGGAT TCCTGAGGCC TGTAACCATC TTTGGACCCA TAGCCGATGT TGCCAGAGAA 
AAGCTGGCCA GAGAAGAGCC AGATATTTAT CAGATTGCAA AAAGTGAACC GCGAGATGCT 
GGAACTGACC AACGTAGCTC TGGCATTATT CGACTTCATA CAATAAAGCA AATAATAGAT 
CAAGACAAGC ATGCCTTATT GGACGTAACA CCAAATGCAG TAGATCGTCT TAATTATGCC 
CAGTGGTATC CAATTGTTGT TTTCCTTAAC CCTGATTCTA AGCAAGGTGT GAAAACAATG 
AGAATGAGGT TATGTCCAGA ATCTCGGAAA AGTGCCAGGA AGTTATATGA GCGCTCTCAC 
AAACTCCGTA AAAACAATCA CCATCTTTTC ACAACTACAA TTAACTTAAA TTCAATGAAC 
GATGGCTGGT ATGGTGCGTT GAAGGAAGCA ATCCAGCAAC AGCAGAACCA GCTGGTATGG 
GTTTCTGAGG GAAAGGCGGA TGGTGCTACA AGTGATGACC TTGATTTGCA TGATGATCGT 
CTGTCCTACC TGTCAGCCCC AGGTAGTGAA TACTCAATGT ATAGCACGGA CAGTAGACAC 
ACTTCTGACT ATGAAGACAC AGATACAGAA GGCGGGGCCT ACACTGATCA AGAACTAGAT 
GAAACTCTTA ATGATGAGGT TGGGACTCCA CCGGAGTCTG CCATTACACG GTCCTCTGAG 
CCTGTAAGAG AGGACTCCTC TGGGATGCAT CATGAAAACC AAACATATCC TCCCTACTCA 
CCACAAGCGC AGCCACAGCC AGTTCCTAGA ATAGACTCCC CGGGATTTAA GACAGCCTCT 
CAACAGAAAG CAGAAGCCTC ATCTCCAGTC CCTTACCTTT CGCCTGAAAC AAACCCAGCA 
TCATCAACCT CTGCTGTTAA TCATAATGTA AATTTAACTA ATGTCAGACT GGAGGAGCCC 
ACCCCAGCTC CTCCCACCTC TCACTCACCA CAAGCTGATT CTTTAAGAAC ACCCAGCACT 
GAGGCAGCTC ACATAATGCT AAGAGATCAA GACCCATCAT TGTCGCCGCA TGTAGATCCA 
GCAAAGGTCT ATAGAAAGGA TCCGTATCCT GAGGAAGTGA TGAGACAGAA TCCAGCTTTG 
AAACAGCCAG CTGTTGGTCA CCCAGGGCAG AGGCCTGAGA AAGAGCCCAG TCTGACCTAT 
GAGCCCCAGC TTCCCTACAT GGAGAAACAA GCCAGCAGAG ACCTCGAGCA GCCCACATAC 
AGATACGAGT CCTCCGGCTA CACAGACCCG TTTTCTCGAA ACTATGACCA TCGCCTGCGA 
TACGAGGACC GCATGCCTGC ATATGAAGAA CAGTGGTCGT ACTACGATGA CAAACAGCCC 
TACCAGCCTC GGCCGCCTTT CGATAGTCAG CACCCTCGAG ACCTTGACCC TAGACAGCAC 
CCTGACGAGC CCTCAGAACG AGGCTATTTC CCACGTTTTG AAGAGCCAGC CCCCCTGTCT 
TATGACAGTC GGCCACGCTA CGAACAGCCG CCCAGAACCT CCACCCTGCG GCATGAAGAG 
CAGCCAGCTC CGGGCTATGA TGTGCACAAC AGACAGAGAC CAGAAGCGCA GCCCTATCCT 
GCAGCAGCCC CTAAGTCGGC GGAGCCCAAG CAGTACTTTG ACCAGTACGG GCGAAGCTAC 
GAGCAAATCC CGCCTCAAGG GTTTGCCTCC AGAACAGGCC ACTACGAACC TCTCCACAGC 
GCCGCAGTCG TCCCTCCGCT CATACCTTCC TCTCAGCATA AGCCAGAAGT CCTGCCTTCC 
AGCACCAAGC CACTGCCCCC ACCTCCAGCC CTAACCGAGG AGGACGAGGA CCCAGCGATG 
AAACCACAGT CTGTGCTCAC CCGAGTGAAA ATGTTTGAAA ACAAAAGGTC TGCATCCCTG 
GAGAACAAGA AGGAGGTGAA TGACACGGCC AGTTTTAAGC CTCCAGAAGC ATCCAAACTT 
CCAGGTGCTC CCGTCACTGG GCCTAAATCC ACCCCTCAGA GCCAGTTCAG TGAACATGAC 
AAAACTTTGT ACAGGATCCC AGAGCCTCAA AAACCGCAAA TGAAGCCTCC TGAAGATATT 
GTCCGGTCAA ATCATTACGA TCCGGAAGAA GATGAAGAGT ATTACCGAAA GCAGCTCTCG 
TACTTTGACC GGAGAAGTTT CGAGAACAAG CCTGCGGCAC ACATTCCTGC CAGCCATCTC 
TCAGAGCCTG CCAAGCCGGC TCATTCTCAG AACCAACCAA ATTTTTCTAG TTATTCTTCA 
AAGGGGAAAG CTGAAGCTGA TGCTCTGGAC CGATCGTTTG GTGAGAAGCG CTATGAGCCA 
GTGCAGCCCA CCCCTCCCCC TCCCCCACTG CCCTCGCAGT ACTCGCAGGC GGCCCAGCCT 
GGCGCCAGCT CTTCTCTCCA CATGCACTCG AAGGGGATAC ATGGTGAAGG TAATTCAATG 
TCATTGGATT TTCAGAATTC TTTGGTATCC AAACCAGACC CACCTCCATC TCAGAGTAAG 
CCAGCAGCTT TCAGAGCACC GCACCGAGAC GATGCCGCTC AGCCTGCTTT ATATCCCCAG 
AAAAGCTTCC CGGAGAAAGC CCCCGTTAAT GGAGCTGAAC AGACTCAGAA AACCATCACT 
CCAGCCTACA ACCGATTCAC ACCAAAACCA TATACCAGTT CTGCCCGGCC ATTCGAGCGC 
AAATTTGAAA GTCCTAAGTT CAACCACAAT CTTCTGCCAG GTGACACTGT ACATAAACCT 
GACTTGTCTG CAAAAGCCCC AGCTTCTCCA AAAACTCTTA TGAAAGCGCA CAGTTCCACA 
CAGCCTCCTG AGTTTGACAG TGGAGTGGAA ACTTTCTCCA TCCATGCAGA GAAGCCCAAG 
TACCAAGTAA CTAATATCAG CACAGTGCCT AGAGCTGCTC CTGTGGGCCC ATCCGCTGTG 
GAAGAAGATG AAGACGAGGA TGGGCACACT GTGGTGGCCA CAGCCCGAGG CATATTTAAC 
AGCAATGGTG GGGTTTTGAG CTCCATAGAG ACCGGCGTCA GTATAATTAT CCCGCAAGGA 
GCCATTCCCG AAGGAATTGA GCAGGAAATC TATTTCAAGG TCTGCCGGGA CAACAGCATC 
CTCCCACCTT TGGATAAAGA GAAAGGTGAA ACCTTGCTAA GCCCCTTAGT GATGTGCGGG 
CCCCATGGCC TCAAGTTCCT GAAGCCCGTG GAGCTGCGCT TGCCACACTG TGCGTCCATG 
ACTCCTGACG GTGATCCTAA AACCTGGCAA AACAAGTGTC TTCCTGGAGA TCCAAATTAC 
CTTGTGGGAG CAAACTGTGT GTCTGTCTTG ATTGACCACT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017348359.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_017203848.1 Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X3, mRNA.

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CloneID	OOb37769
Clone ID Related Accession (Same CDS sequence)	XM_017348359.1
Accession Version	XM_017348359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013685.1) and transcript sequence (GBCI01037002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 477 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCCGCCA GAGTGGCGGC CGCCAAGAAC ACGGCCATGG AGGAAACAGC TATATGGGAA 
CAACACACAG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCGGGT 
GGAAGAGATA ACCCTCATTT TCAGAGTGGA GAGACCTCCA TAGTAATTTC AGATGTGCTG 
AAGGGAGGAC CAGCCGAGGG GCAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGG 
GTTTCCATGG ATAACGTTGA ACATGCCTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAGA TTACCATTCG ACGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TGTCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATCCA TGACCCGAGA 
AGTGGCCGCA GTGGTCTGGC AAACAGAAGG AGTGAGAAGA GTTGGGCAAG GGATAGAAGT 
GCGAGTCGAG AGAGGAGCTT GTCTCCGCGA TCAGATAGGC GGTCGGTGGC CTCTAGCCAG 
CCTGCGAAAC CCACCAAAGT CACTTTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCACATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAACATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACGGTGAC AGAAAATATG 
TCATTGACTG ATGCGAAGAC GTTGATAGAA AGGTCTAAAG GCAAGTTGAA AATGGTAGTC 
CAGAGAGATG AACGGGCGAC CTTGCTGAAC GTCCCTGACC TTTCTGACAG CATCCATTCT 
GCTAATGCCT CCGAGAGAGA TGACATTTCA GAAATCCAGT CACTGGCATC AGATCATTCT 
GGCCGGTCAC ATGACAGGCC TCCACGCCGC AGCAGCCGGT CCCGCTCTCC TGACCAGCGG 
TCTGAGCCTT CCGACCACTC CAGACAGTCT CCGCAGCAGC CCAGCAATGG CAGTCTCCGG 
AGCAGAGAAG AAGAGAGAAT TTCTAAGCCT GGGGCTGTCT CGACTCCTGT AAAGCATGGA 
GATGACCACA CACCTAAAAC AGTGGAAGAA ATTACTGTTG AGAGAAATGA GAAGCAAACA 
CCTGCCCTTC CAGAACCAAA GCCTGTGTAC GCTCAAGTAG GACAACCAGA CGTGGATTTA 
CCTGTCAGCC CATCTGATGG TGTCCTGCCT AACTCAGCGC ACGAGGACGG GATGCTTCGG 
CCCAGCATGA AATTGGTAAA ATTCAGAAAA GGAGATAGCG TGGGTCTGCG GCTGGCTGGT 
GGAAATGATG TCGGAATATT TGTGGCTGGC GTTCTGGAAG ATAGCCCTGC TGCTAAAGAA 
GGCTTAGAGG AGGGTGATCA GATTCTCAGG GTAAACAATG TGGATTTCAC CAATATCATT 
AGAGAAGAAG CTGTCCTTTT CCTGCTTGAC CTCCCTAAAG GAGAAGAAGT GACCATCCTG 
GCCCAGAAGA AGAAGGATGT TTATCGTCGC ATTGTAGAAT CAGATGTTGG AGATTCTTTC 
TATATTAGAA CCCATTTTGA ATACGAAAAG GAATCTCCCT ATGGACTTAG TTTTAACAAG 
GGAGAGGTAT TTCGTGTTGT GGATACCTTG TACAATGGAA AACTGGGCTC TTGGCTTGCT 
ATTCGAATTG GCAAAAATCA TAAGGAGGTG GAACGAGGCA TCATCCCTAA TAAGAACAGA 
GCTGAACAGC TGGCCAGTGT GCAGTATACA CTTCCCAAAA CTGCGGGGGG CGATCGTGCG 
GACTTCTGGA GGTTCCGGGG TCTCCGCAGC TCCAAGAGGA ATCTTCGGAA AAGCAGAGAG 
GATTTGTCAG CCCAGCCAGT TCAGACGAAA TTTCCAGCTT ATGAAAGGGT TGTTCTTCGA 
GAAGCTGGAT TCCTGAGGCC TGTAACCATC TTTGGACCCA TAGCCGATGT TGCCAGAGAA 
AAGCTGGCCA GAGAAGAGCC AGATATTTAT CAGATTGCAA AAAGTGAACC GCGAGATGCT 
GGAACTGACC AACGTAGCTC TGGCATTATT CGACTTCATA CAATAAAGCA AATAATAGAT 
CAAGACAAGC ATGCCTTATT GGACGTAACA CCAAATGCAG TAGATCGTCT TAATTATGCC 
CAGTGGTATC CAATTGTTGT TTTCCTTAAC CCTGATTCTA AGCAAGGTGT GAAAACAATG 
AGAATGAGGT TATGTCCAGA ATCTCGGAAA AGTGCCAGGA AGTTATATGA GCGCTCTCAC 
AAACTCCGTA AAAACAATCA CCATCTTTTC ACAACTACAA TTAACTTAAA TTCAATGAAC 
GATGGCTGGT ATGGTGCGTT GAAGGAAGCA ATCCAGCAAC AGCAGAACCA GCTGGTATGG 
GTTTCTGAGG GAAAGGCGGA TGGTGCTACA AGTGATGACC TTGATTTGCA TGATGATCGT 
CTGTCCTACC TGTCAGCCCC AGGTAGTGAA TACTCAATGT ATAGCACGGA CAGTAGACAC 
ACTTCTGACT ATGAAGACAC AGATACAGAA GGCGGGGCCT ACACTGATCA AGAACTAGAT 
GAAACTCTTA ATGATGAGGT TGGGACTCCA CCGGAGTCTG CCATTACACG GTCCTCTGAG 
CCTGTAAGAG AGGACTCCTC TGGGATGCAT CATGAAAACC AAACATATCC TCCCTACTCA 
CCACAAGCGC AGCCACAGCC AGTTCCTAGA ATAGACTCCC CGGGATTTAA GACAGCCTCT 
CAACAGAAAG CAGAAGCCTC ATCTCCAGTC CCTTACCTTT CGCCTGAAAC AAACCCAGCA 
TCATCAACCT CTGCTGTTAA TCATAATGTA AATTTAACTA ATGTCAGACT GGAGGAGCCC 
ACCCCAGCTC CTCCCACCTC TCACTCACCA CAAGCTGATT CTTTAAGAAC ACCCAGCACT 
GAGGCAGCTC ACATAATGCT AAGAGATCAA GACCCATCAT TGTCGCCGCA TGTAGATCCA 
GCAAAGGTCT ATAGAAAGGA TCCGTATCCT GAGGAAGTGA TGAGACAGAA TCCAGCTTTG 
AAACAGCCAG CTGTTGGTCA CCCAGGGCAG AGGCCTGAGA AAGAGCCCAG TCTGACCTAT 
GAGCCCCAGC TTCCCTACAT GGAGAAACAA GCCAGCAGAG ACCTCGAGCA GCCCACATAC 
AGATACGAGT CCTCCGGCTA CACAGACCCG TTTTCTCGAA ACTATGACCA TCGCCTGCGA 
TACGAGGACC GCATGCCTGC ATATGAAGAA CAGTGGTCGT ACTACGATGA CAAACAGCCC 
TACCAGCCTC GGCCGCCTTT CGATAGTCAG CACCCTCGAG ACCTTGACCC TAGACAGCAC 
CCTGACGAGC CCTCAGAACG AGGCTATTTC CCACGTTTTG AAGAGCCAGC CCCCCTGTCT 
TATGACAGTC GGCCACGCTA CGAACAGCCG CCCAGAACCT CCACCCTGCG GCATGAAGAG 
CAGCCAGCTC CGGGCTATGA TGTGCACAAC AGACAGAGAC CAGAAGCGCA GCCCTATCCT 
GCAGCAGCCC CTAAGTCGGC GGAGCCCAAG CAGTACTTTG ACCAGTACGG GCGAAGCTAC 
GAGCAAATCC CGCCTCAAGG GTTTGCCTCC AGAACAGGCC ACTACGAACC TCTCCACAGC 
GCCGCAGTCG TCCCTCCGCT CATACCTTCC TCTCAGCATA AGCCAGAAGT CCTGCCTTCC 
AGCACCAAGC CACTGCCCCC ACCTCCAGCC CTAACCGAGG AGGACGAGGA CCCAGCGATG 
AAACCACAGT CTGTGCTCAC CCGAGTGAAA ATGTTTGAAA ACAAAAGGTC TGCATCCCTG 
GAGAACAAGA AGGAGGTGAA TGACACGGCC AGTTTTAAGC CTCCAGAAGC ATCCAAACTT 
CCAGGTGCTC CCGTCACTGG GCCTAAATCC ACCCCTCAGA GCCAGTTCAG TGAACATGAC 
AAAACTTTGT ACAGGATCCC AGAGCCTCAA AAACCGCAAA TGAAGCCTCC TGAAGATATT 
GTCCGGTCAA ATCATTACGA TCCGGAAGAA GATGAAGAGT ATTACCGAAA GCAGCTCTCG 
TACTTTGACC GGAGAAGTTT CGAGAACAAG CCTGCGGCAC ACATTCCTGC CAGCCATCTC 
TCAGAGCCTG CCAAGCCGGC TCATTCTCAG AACCAACCAA ATTTTTCTAG TTATTCTTCA 
AAGGGGAAAG CTGAAGCTGA TGCTCTGGAC CGATCGTTTG GTGAGAAGCG CTATGAGCCA 
GTGCAGCCCA CCCCTCCCCC TCCCCCACTG CCCTCGCAGT ACTCGCAGGC GGCCCAGCCT 
GGCGCCAGCT CTTCTCTCCA CATGCACTCG AAGGGGATAC ATGGTGAAGG TAATTCAATG 
TCATTGGATT TTCAGAATTC TTTGGTATCC AAACCAGACC CACCTCCATC TCAGAGTAAG 
CCAGCAGCTT TCAGAGCACC GCACCGAGAC GATGCCGCTC AGCCTGCTTT ATATCCCCAG 
AAAAGCTTCC CGGAGAAAGC CCCCGTTAAT GGAGCTGAAC AGACTCAGAA AACCATCACT 
CCAGCCTACA ACCGATTCAC ACCAAAACCA TATACCAGTT CTGCCCGGCC ATTCGAGCGC 
AAATTTGAAA GTCCTAAGTT CAACCACAAT CTTCTGCCAG GTGACACTGT ACATAAACCT 
GACTTGTCTG CAAAAGCCCC AGCTTCTCCA AAAACTCTTA TGAAAGCGCA CAGTTCCACA 
CAGCCTCCTG AGTTTGACAG TGGAGTGGAA ACTTTCTCCA TCCATGCAGA GAAGCCCAAG 
TACCAAGTAA CTAATATCAG CACAGTGCCT AGAGCTGCTC CTGTGGGCCC ATCCGCTGTG 
GAAGAAGATG AAGACGAGGA TGGGCACACT GTGGTGGCCA CAGCCCGAGG CATATTTAAC 
AGCAATGGTG GGGTTTTGAG CTCCATAGAG ACCGGCGTCA GTATAATTAT CCCGCAAGGA 
GCCATTCCCG AAGGAATTGA GCAGGAAATC TATTTCAAGG TCTGCCGGGA CAACAGCATC 
CTCCCACCTT TGGATAAAGA GAAAGGTGAA ACCTTGCTAA GCCCCTTAGT GATGTGCGGG 
CCCCATGGCC TCAAGTTCCT GAAGCCCGTG GAGCTGCGCT TGCCACACTG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTACC TTGTGGGAGC AAACTGTGTG 
TCTGTCTTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017203848.1
CDS	451..5694
Translation	MSARVAAAKNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGLANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSSRSRSPDQRSEPSDHSRQSPQQPSNGSLRSREEERISKPGAVSTPVKHGDDHTPKTVEEITVERNEKQTPALPEPKPVYAQVGQPDVDLPVSPSDGVLPNSAHEDGMLRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVPRIDSPGFKTASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPPTSHSPQADSLRTPSTEAAHIMLRDQDPSLSPHVDPAKVYRKDPYPEEVMRQNPALKQPAVGHPGQRPEKEPSLTYEPQLPYMEKQASRDLEQPTYRYESSGYTDPFSRNYDHRLRYEDRMPAYEEQWSYYDDKQPYQPRPPFDSQHPRDLDPRQHPDEPSERGYFPRFEEPAPLSYDSRPRYEQPPRTSTLRHEEQPAPGYDVHNRQRPEAQPYPAAAPKSAEPKQYFDQYGRSYEQIPPQGFASRTGHYEPLHSAAVVPPLIPSSQHKPEVLPSSTKPLPPPPALTEEDEDPAMKPQSVLTRVKMFENKRSASLENKKEVNDTASFKPPEASKLPGAPVTGPKSTPQSQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPAAHIPASHLSEPAKPAHSQNQPNFSSYSSKGKAEADALDRSFGEKRYEPVQPTPPPPPLPSQYSQAAQPGASSSLHMHSKGIHGEGNSMSLDFQNSLVSKPDPPPSQSKPAAFRAPHRDDAAQPALYPQKSFPEKAPVNGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPGDTVHKPDLSAKAPASPKTLMKAHSSTQPPEFDSGVETFSIHAEKPKYQVTNISTVPRAAPVGPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017348359.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017348359.1

ORF Insert Sequence:

ATGTCCGCCA GAGTGGCGGC CGCCAAGAAC ACGGCCATGG AGGAAACAGC TATATGGGAA 
CAACACACAG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCGGGT 
GGAAGAGATA ACCCTCATTT TCAGAGTGGA GAGACCTCCA TAGTAATTTC AGATGTGCTG 
AAGGGAGGAC CAGCCGAGGG GCAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGG 
GTTTCCATGG ATAACGTTGA ACATGCCTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAGA TTACCATTCG ACGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TGTCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATCCA TGACCCGAGA 
AGTGGCCGCA GTGGTCTGGC AAACAGAAGG AGTGAGAAGA GTTGGGCAAG GGATAGAAGT 
GCGAGTCGAG AGAGGAGCTT GTCTCCGCGA TCAGATAGGC GGTCGGTGGC CTCTAGCCAG 
CCTGCGAAAC CCACCAAAGT CACTTTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCACATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAACATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACGGTGAC AGAAAATATG 
TCATTGACTG ATGCGAAGAC GTTGATAGAA AGGTCTAAAG GCAAGTTGAA AATGGTAGTC 
CAGAGAGATG AACGGGCGAC CTTGCTGAAC GTCCCTGACC TTTCTGACAG CATCCATTCT 
GCTAATGCCT CCGAGAGAGA TGACATTTCA GAAATCCAGT CACTGGCATC AGATCATTCT 
GGCCGGTCAC ATGACAGGCC TCCACGCCGC AGCAGCCGGT CCCGCTCTCC TGACCAGCGG 
TCTGAGCCTT CCGACCACTC CAGACAGTCT CCGCAGCAGC CCAGCAATGG CAGTCTCCGG 
AGCAGAGAAG AAGAGAGAAT TTCTAAGCCT GGGGCTGTCT CGACTCCTGT AAAGCATGGA 
GATGACCACA CACCTAAAAC AGTGGAAGAA ATTACTGTTG AGAGAAATGA GAAGCAAACA 
CCTGCCCTTC CAGAACCAAA GCCTGTGTAC GCTCAAGTAG GACAACCAGA CGTGGATTTA 
CCTGTCAGCC CATCTGATGG TGTCCTGCCT AACTCAGCGC ACGAGGACGG GATGCTTCGG 
CCCAGCATGA AATTGGTAAA ATTCAGAAAA GGAGATAGCG TGGGTCTGCG GCTGGCTGGT 
GGAAATGATG TCGGAATATT TGTGGCTGGC GTTCTGGAAG ATAGCCCTGC TGCTAAAGAA 
GGCTTAGAGG AGGGTGATCA GATTCTCAGG GTAAACAATG TGGATTTCAC CAATATCATT 
AGAGAAGAAG CTGTCCTTTT CCTGCTTGAC CTCCCTAAAG GAGAAGAAGT GACCATCCTG 
GCCCAGAAGA AGAAGGATGT TTATCGTCGC ATTGTAGAAT CAGATGTTGG AGATTCTTTC 
TATATTAGAA CCCATTTTGA ATACGAAAAG GAATCTCCCT ATGGACTTAG TTTTAACAAG 
GGAGAGGTAT TTCGTGTTGT GGATACCTTG TACAATGGAA AACTGGGCTC TTGGCTTGCT 
ATTCGAATTG GCAAAAATCA TAAGGAGGTG GAACGAGGCA TCATCCCTAA TAAGAACAGA 
GCTGAACAGC TGGCCAGTGT GCAGTATACA CTTCCCAAAA CTGCGGGGGG CGATCGTGCG 
GACTTCTGGA GGTTCCGGGG TCTCCGCAGC TCCAAGAGGA ATCTTCGGAA AAGCAGAGAG 
GATTTGTCAG CCCAGCCAGT TCAGACGAAA TTTCCAGCTT ATGAAAGGGT TGTTCTTCGA 
GAAGCTGGAT TCCTGAGGCC TGTAACCATC TTTGGACCCA TAGCCGATGT TGCCAGAGAA 
AAGCTGGCCA GAGAAGAGCC AGATATTTAT CAGATTGCAA AAAGTGAACC GCGAGATGCT 
GGAACTGACC AACGTAGCTC TGGCATTATT CGACTTCATA CAATAAAGCA AATAATAGAT 
CAAGACAAGC ATGCCTTATT GGACGTAACA CCAAATGCAG TAGATCGTCT TAATTATGCC 
CAGTGGTATC CAATTGTTGT TTTCCTTAAC CCTGATTCTA AGCAAGGTGT GAAAACAATG 
AGAATGAGGT TATGTCCAGA ATCTCGGAAA AGTGCCAGGA AGTTATATGA GCGCTCTCAC 
AAACTCCGTA AAAACAATCA CCATCTTTTC ACAACTACAA TTAACTTAAA TTCAATGAAC 
GATGGCTGGT ATGGTGCGTT GAAGGAAGCA ATCCAGCAAC AGCAGAACCA GCTGGTATGG 
GTTTCTGAGG GAAAGGCGGA TGGTGCTACA AGTGATGACC TTGATTTGCA TGATGATCGT 
CTGTCCTACC TGTCAGCCCC AGGTAGTGAA TACTCAATGT ATAGCACGGA CAGTAGACAC 
ACTTCTGACT ATGAAGACAC AGATACAGAA GGCGGGGCCT ACACTGATCA AGAACTAGAT 
GAAACTCTTA ATGATGAGGT TGGGACTCCA CCGGAGTCTG CCATTACACG GTCCTCTGAG 
CCTGTAAGAG AGGACTCCTC TGGGATGCAT CATGAAAACC AAACATATCC TCCCTACTCA 
CCACAAGCGC AGCCACAGCC AGTTCCTAGA ATAGACTCCC CGGGATTTAA GACAGCCTCT 
CAACAGAAAG CAGAAGCCTC ATCTCCAGTC CCTTACCTTT CGCCTGAAAC AAACCCAGCA 
TCATCAACCT CTGCTGTTAA TCATAATGTA AATTTAACTA ATGTCAGACT GGAGGAGCCC 
ACCCCAGCTC CTCCCACCTC TCACTCACCA CAAGCTGATT CTTTAAGAAC ACCCAGCACT 
GAGGCAGCTC ACATAATGCT AAGAGATCAA GACCCATCAT TGTCGCCGCA TGTAGATCCA 
GCAAAGGTCT ATAGAAAGGA TCCGTATCCT GAGGAAGTGA TGAGACAGAA TCCAGCTTTG 
AAACAGCCAG CTGTTGGTCA CCCAGGGCAG AGGCCTGAGA AAGAGCCCAG TCTGACCTAT 
GAGCCCCAGC TTCCCTACAT GGAGAAACAA GCCAGCAGAG ACCTCGAGCA GCCCACATAC 
AGATACGAGT CCTCCGGCTA CACAGACCCG TTTTCTCGAA ACTATGACCA TCGCCTGCGA 
TACGAGGACC GCATGCCTGC ATATGAAGAA CAGTGGTCGT ACTACGATGA CAAACAGCCC 
TACCAGCCTC GGCCGCCTTT CGATAGTCAG CACCCTCGAG ACCTTGACCC TAGACAGCAC 
CCTGACGAGC CCTCAGAACG AGGCTATTTC CCACGTTTTG AAGAGCCAGC CCCCCTGTCT 
TATGACAGTC GGCCACGCTA CGAACAGCCG CCCAGAACCT CCACCCTGCG GCATGAAGAG 
CAGCCAGCTC CGGGCTATGA TGTGCACAAC AGACAGAGAC CAGAAGCGCA GCCCTATCCT 
GCAGCAGCCC CTAAGTCGGC GGAGCCCAAG CAGTACTTTG ACCAGTACGG GCGAAGCTAC 
GAGCAAATCC CGCCTCAAGG GTTTGCCTCC AGAACAGGCC ACTACGAACC TCTCCACAGC 
GCCGCAGTCG TCCCTCCGCT CATACCTTCC TCTCAGCATA AGCCAGAAGT CCTGCCTTCC 
AGCACCAAGC CACTGCCCCC ACCTCCAGCC CTAACCGAGG AGGACGAGGA CCCAGCGATG 
AAACCACAGT CTGTGCTCAC CCGAGTGAAA ATGTTTGAAA ACAAAAGGTC TGCATCCCTG 
GAGAACAAGA AGGAGGTGAA TGACACGGCC AGTTTTAAGC CTCCAGAAGC ATCCAAACTT 
CCAGGTGCTC CCGTCACTGG GCCTAAATCC ACCCCTCAGA GCCAGTTCAG TGAACATGAC 
AAAACTTTGT ACAGGATCCC AGAGCCTCAA AAACCGCAAA TGAAGCCTCC TGAAGATATT 
GTCCGGTCAA ATCATTACGA TCCGGAAGAA GATGAAGAGT ATTACCGAAA GCAGCTCTCG 
TACTTTGACC GGAGAAGTTT CGAGAACAAG CCTGCGGCAC ACATTCCTGC CAGCCATCTC 
TCAGAGCCTG CCAAGCCGGC TCATTCTCAG AACCAACCAA ATTTTTCTAG TTATTCTTCA 
AAGGGGAAAG CTGAAGCTGA TGCTCTGGAC CGATCGTTTG GTGAGAAGCG CTATGAGCCA 
GTGCAGCCCA CCCCTCCCCC TCCCCCACTG CCCTCGCAGT ACTCGCAGGC GGCCCAGCCT 
GGCGCCAGCT CTTCTCTCCA CATGCACTCG AAGGGGATAC ATGGTGAAGG TAATTCAATG 
TCATTGGATT TTCAGAATTC TTTGGTATCC AAACCAGACC CACCTCCATC TCAGAGTAAG 
CCAGCAGCTT TCAGAGCACC GCACCGAGAC GATGCCGCTC AGCCTGCTTT ATATCCCCAG 
AAAAGCTTCC CGGAGAAAGC CCCCGTTAAT GGAGCTGAAC AGACTCAGAA AACCATCACT 
CCAGCCTACA ACCGATTCAC ACCAAAACCA TATACCAGTT CTGCCCGGCC ATTCGAGCGC 
AAATTTGAAA GTCCTAAGTT CAACCACAAT CTTCTGCCAG GTGACACTGT ACATAAACCT 
GACTTGTCTG CAAAAGCCCC AGCTTCTCCA AAAACTCTTA TGAAAGCGCA CAGTTCCACA 
CAGCCTCCTG AGTTTGACAG TGGAGTGGAA ACTTTCTCCA TCCATGCAGA GAAGCCCAAG 
TACCAAGTAA CTAATATCAG CACAGTGCCT AGAGCTGCTC CTGTGGGCCC ATCCGCTGTG 
GAAGAAGATG AAGACGAGGA TGGGCACACT GTGGTGGCCA CAGCCCGAGG CATATTTAAC 
AGCAATGGTG GGGTTTTGAG CTCCATAGAG ACCGGCGTCA GTATAATTAT CCCGCAAGGA 
GCCATTCCCG AAGGAATTGA GCAGGAAATC TATTTCAAGG TCTGCCGGGA CAACAGCATC 
CTCCCACCTT TGGATAAAGA GAAAGGTGAA ACCTTGCTAA GCCCCTTAGT GATGTGCGGG 
CCCCATGGCC TCAAGTTCCT GAAGCCCGTG GAGCTGCGCT TGCCACACTG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTACC TTGTGGGAGC AAACTGTGTG 
TCTGTCTTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017348360.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_017203849.1 Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X4, mRNA.

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CloneID	OOb37770
Clone ID Related Accession (Same CDS sequence)	XM_017348360.1
Accession Version	XM_017348360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013685.1) and transcript sequence (GBCI01037002.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 477 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTCCGCCA GAGTGGCGGC CGCCAAGAAC ACGGCCATGG AGGAAACAGC TATATGGGAA 
CAACACACAG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCGGGT 
GGAAGAGATA ACCCTCATTT TCAGAGTGGA GAGACCTCCA TAGTAATTTC AGATGTGCTG 
AAGGGAGGAC CAGCCGAGGG GCAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGG 
GTTTCCATGG ATAACGTTGA ACATGCCTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAGA TTACCATTCG ACGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TGTCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATCCA TGACCCGAGA 
AGTGGCCGCA GTGGTCTGGC AAACAGAAGG AGTGAGAAGA GTTGGGCAAG GGATAGAAGT 
GCGAGTCGAG AGAGGAGCTT GTCTCCGCGA TCAGATAGGC GGTCGGTGGC CTCTAGCCAG 
CCTGCGAAAC CCACCAAAGT CACTTTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCACATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAACATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACGGTGAC AGAAAATATG 
TCATTGACTG ATGCGAAGAC GTTGATAGAA AGGTCTAAAG GCAAGTTGAA AATGGTAGTC 
CAGAGAGATG AACGGGCGAC CTTGCTGAAC GTCCCTGACC TTTCTGACAG CATCCATTCT 
GCTAATGCCT CCGAGAGAGA TGACATTTCA GAAATCCAGT CACTGGCATC AGATCATTCT 
GGCCGGTCAC ATGACAGGCC TCCACGCCGC AGCAGCCGGT CCCGCTCTCC TGACCAGCGG 
TCTGAGCCTT CCGACCACTC CAGACAGTCT CCGCAGCAGC CCAGCAATGG CAGTCTCCGG 
AGCAGAGAAG AAGAGAGAAT TTCTAAGCCT GGGGCTGTCT CGACTCCTGT AAAGCATGGA 
GATGACCACA CACCTAAAAC AGTGGAAGAA ATTACTGTTG AGAGAAATGA GAAGCAAACA 
CCTGCCCTTC CAGAACCAAA GCCTGTGTAC GCTCAAGTAG GACAACCAGA CGTGGATTTA 
CCTGTCAGCC CATCTGATGG TGTCCTGCCT AACTCAGCGC ACGAGGACGG GATGCTTCGG 
CCCAGCATGA AATTGGTAAA ATTCAGAAAA GGAGATAGCG TGGGTCTGCG GCTGGCTGGT 
GGAAATGATG TCGGAATATT TGTGGCTGGC GTTCTGGAAG ATAGCCCTGC TGCTAAAGAA 
GGCTTAGAGG AGGGTGATCA GATTCTCAGG GTAAACAATG TGGATTTCAC CAATATCATT 
AGAGAAGAAG CTGTCCTTTT CCTGCTTGAC CTCCCTAAAG GAGAAGAAGT GACCATCCTG 
GCCCAGAAGA AGAAGGATGT TTATCGTCGC ATTGTAGAAT CAGATGTTGG AGATTCTTTC 
TATATTAGAA CCCATTTTGA ATACGAAAAG GAATCTCCCT ATGGACTTAG TTTTAACAAG 
GGAGAGGTAT TTCGTGTTGT GGATACCTTG TACAATGGAA AACTGGGCTC TTGGCTTGCT 
ATTCGAATTG GCAAAAATCA TAAGGAGGTG GAACGAGGCA TCATCCCTAA TAAGAACAGA 
GCTGAACAGC TGGCCAGTGT GCAGTATACA CTTCCCAAAA CTGCGGGGGG CGATCGTGCG 
GACTTCTGGA GGTTCCGGGG TCTCCGCAGC TCCAAGAGGA ATCTTCGGAA AAGCAGAGAG 
GATTTGTCAG CCCAGCCAGT TCAGACGAAA TTTCCAGCTT ATGAAAGGGT TGTTCTTCGA 
GAAGCTGGAT TCCTGAGGCC TGTAACCATC TTTGGACCCA TAGCCGATGT TGCCAGAGAA 
AAGCTGGCCA GAGAAGAGCC AGATATTTAT CAGATTGCAA AAAGTGAACC GCGAGATGCT 
GGAACTGACC AACGTAGCTC TGGCATTATT CGACTTCATA CAATAAAGCA AATAATAGAT 
CAAGACAAGC ATGCCTTATT GGACGTAACA CCAAATGCAG TAGATCGTCT TAATTATGCC 
CAGTGGTATC CAATTGTTGT TTTCCTTAAC CCTGATTCTA AGCAAGGTGT GAAAACAATG 
AGAATGAGGT TATGTCCAGA ATCTCGGAAA AGTGCCAGGA AGTTATATGA GCGCTCTCAC 
AAACTCCGTA AAAACAATCA CCATCTTTTC ACAACTACAA TTAACTTAAA TTCAATGAAC 
GATGGCTGGT ATGGTGCGTT GAAGGAAGCA ATCCAGCAAC AGCAGAACCA GCTGGTATGG 
GTTTCTGAGG GAAAGGCGGA TGGTGCTACA AGTGATGACC TTGATTTGCA TGATGATCGT 
CTGTCCTACC TGTCAGCCCC AGGTAGTGAA TACTCAATGT ATAGCACGGA CAGTAGACAC 
ACTTCTGACT ATGAAGACAC AGATACAGAA GGCGGGGCCT ACACTGATCA AGAACTAGAT 
GAAACTCTTA ATGATGAGGT TGGGACTCCA CCGGAGTCTG CCATTACACG GTCCTCTGAG 
CCTGTAAGAG AGGACTCCTC TGGGATGCAT CATGAAAACC AAACATATCC TCCCTACTCA 
CCACAAGCGC AGCCACAGCC AGTTCCTAGA ATAGACTCCC CGGGATTTAA GACAGCCTCT 
CAACAGGTCT ATAGAAAGGA TCCGTATCCT GAGGAAGTGA TGAGACAGAA TCCAGCTTTG 
AAACAGCCAG CTGTTGGTCA CCCAGGGCAG AGGCCTGAGA AAGAGCCCAG TCTGACCTAT 
GAGCCCCAGC TTCCCTACAT GGAGAAACAA GCCAGCAGAG ACCTCGAGCA GCCCACATAC 
AGATACGAGT CCTCCGGCTA CACAGACCCG TTTTCTCGAA ACTATGACCA TCGCCTGCGA 
TACGAGGACC GCATGCCTGC ATATGAAGAA CAGTGGTCGT ACTACGATGA CAAACAGCCC 
TACCAGCCTC GGCCGCCTTT CGATAGTCAG CACCCTCGAG ACCTTGACCC TAGACAGCAC 
CCTGACGAGC CCTCAGAACG AGGCTATTTC CCACGTTTTG AAGAGCCAGC CCCCCTGTCT 
TATGACAGTC GGCCACGCTA CGAACAGCCG CCCAGAACCT CCACCCTGCG GCATGAAGAG 
CAGCCAGCTC CGGGCTATGA TGTGCACAAC AGACAGAGAC CAGAAGCGCA GCCCTATCCT 
GCAGCAGCCC CTAAGTCGGC GGAGCCCAAG CAGTACTTTG ACCAGTACGG GCGAAGCTAC 
GAGCAAATCC CGCCTCAAGG GTTTGCCTCC AGAACAGGCC ACTACGAACC TCTCCACAGC 
GCCGCAGTCG TCCCTCCGCT CATACCTTCC TCTCAGCATA AGCCAGAAGT CCTGCCTTCC 
AGCACCAAGC CACTGCCCCC ACCTCCAGCC CTAACCGAGG AGGACGAGGA CCCAGCGATG 
AAACCACAGT CTGTGCTCAC CCGAGTGAAA ATGTTTGAAA ACAAAAGGTC TGCATCCCTG 
GAGAACAAGA AGGAGGTGAA TGACACGGCC AGTTTTAAGC CTCCAGAAGC ATCCAAACTT 
CCAGGTGCTC CCGTCACTGG GCCTAAATCC ACCCCTCAGA GCCAGTTCAG TGAACATGAC 
AAAACTTTGT ACAGGATCCC AGAGCCTCAA AAACCGCAAA TGAAGCCTCC TGAAGATATT 
GTCCGGTCAA ATCATTACGA TCCGGAAGAA GATGAAGAGT ATTACCGAAA GCAGCTCTCG 
TACTTTGACC GGAGAAGTTT CGAGAACAAG CCTGCGGCAC ACATTCCTGC CAGCCATCTC 
TCAGAGCCTG CCAAGCCGGC TCATTCTCAG AACCAACCAA ATTTTTCTAG TTATTCTTCA 
AAGGGGAAAG CTGAAGCTGA TGCTCTGGAC CGATCGTTTG GTGAGAAGCG CTATGAGCCA 
GTGCAGCCCA CCCCTCCCCC TCCCCCACTG CCCTCGCAGT ACTCGCAGGC GGCCCAGCCT 
GGCGCCAGCT CTTCTCTCCA CATGCACTCG AAGGGGATAC ATGGTGAAGG TAATTCAATG 
TCATTGGATT TTCAGAATTC TTTGGTATCC AAACCAGACC CACCTCCATC TCAGAGTAAG 
CCAGCAGCTT TCAGAGCACC GCACCGAGAC GATGCCGCTC AGCCTGCTTT ATATCCCCAG 
AAAAGCTTCC CGGAGAAAGC CCCCGTTAAT GGAGCTGAAC AGACTCAGAA AACCATCACT 
CCAGCCTACA ACCGATTCAC ACCAAAACCA TATACCAGTT CTGCCCGGCC ATTCGAGCGC 
AAATTTGAAA GTCCTAAGTT CAACCACAAT CTTCTGCCAG GTGACACTGT ACATAAACCT 
GACTTGTCTG CAAAAGCCCC AGCTTCTCCA AAAACTCTTA TGAAAGCGCA CAGTTCCACA 
CAGCCTCCTG AGTTTGACAG TGGAGTGGAA ACTTTCTCCA TCCATGCAGA GAAGCCCAAG 
TACCAAGTAA CTAATATCAG CACAGTGCCT AGAGCTGCTC CTGTGGGCCC ATCCGCTGTG 
GAAGAAGATG AAGACGAGGA TGGGCACACT GTGGTGGCCA CAGCCCGAGG CATATTTAAC 
AGCAATGGTG GGGTTTTGAG CTCCATAGAG ACCGGCGTCA GTATAATTAT CCCGCAAGGA 
GCCATTCCCG AAGGAATTGA GCAGGAAATC TATTTCAAGG TCTGCCGGGA CAACAGCATC 
CTCCCACCTT TGGATAAAGA GAAAGGTGAA ACCTTGCTAA GCCCCTTAGT GATGTGCGGG 
CCCCATGGCC TCAAGTTCCT GAAGCCCGTG GAGCTGCGCT TGCCACACTG TGCGTCCATG 
ACTCCTGACG GTTGGTCTTT TGCTCTAAAA TCATCCGACT CCTCGTCGGG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTACC TTGTGGGAGC AAACTGTGTG 
TCTGTCTTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017203849.1
CDS	451..5514
Translation	MSARVAAAKNTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGLANRRSEKSWARDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSSRSRSPDQRSEPSDHSRQSPQQPSNGSLRSREEERISKPGAVSTPVKHGDDHTPKTVEEITVERNEKQTPALPEPKPVYAQVGQPDVDLPVSPSDGVLPNSAHEDGMLRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPVPRIDSPGFKTASQQVYRKDPYPEEVMRQNPALKQPAVGHPGQRPEKEPSLTYEPQLPYMEKQASRDLEQPTYRYESSGYTDPFSRNYDHRLRYEDRMPAYEEQWSYYDDKQPYQPRPPFDSQHPRDLDPRQHPDEPSERGYFPRFEEPAPLSYDSRPRYEQPPRTSTLRHEEQPAPGYDVHNRQRPEAQPYPAAAPKSAEPKQYFDQYGRSYEQIPPQGFASRTGHYEPLHSAAVVPPLIPSSQHKPEVLPSSTKPLPPPPALTEEDEDPAMKPQSVLTRVKMFENKRSASLENKKEVNDTASFKPPEASKLPGAPVTGPKSTPQSQFSEHDKTLYRIPEPQKPQMKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPAAHIPASHLSEPAKPAHSQNQPNFSSYSSKGKAEADALDRSFGEKRYEPVQPTPPPPPLPSQYSQAAQPGASSSLHMHSKGIHGEGNSMSLDFQNSLVSKPDPPPSQSKPAAFRAPHRDDAAQPALYPQKSFPEKAPVNGAEQTQKTITPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPGDTVHKPDLSAKAPASPKTLMKAHSSTQPPEFDSGVETFSIHAEKPKYQVTNISTVPRAAPVGPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGIEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017348360.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus tight junction protein 1 (TJP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017348360.1

ORF Insert Sequence:

ATGTCCGCCA GAGTGGCGGC CGCCAAGAAC ACGGCCATGG AGGAAACAGC TATATGGGAA 
CAACACACAG TGACACTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCGGGT 
GGAAGAGATA ACCCTCATTT TCAGAGTGGA GAGACCTCCA TAGTAATTTC AGATGTGCTG 
AAGGGAGGAC CAGCCGAGGG GCAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAATGGG 
GTTTCCATGG ATAACGTTGA ACATGCCTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAGA TTACCATTCG ACGGAAGAAG AAAGTTCAGA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TGTCTGATAA TGAAGAAGAT AGTTACGATG AGGAAATCCA TGACCCGAGA 
AGTGGCCGCA GTGGTCTGGC AAACAGAAGG AGTGAGAAGA GTTGGGCAAG GGATAGAAGT 
GCGAGTCGAG AGAGGAGCTT GTCTCCGCGA TCAGATAGGC GGTCGGTGGC CTCTAGCCAG 
CCTGCGAAAC CCACCAAAGT CACTTTGGTG AAGTCTCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCCA GCCACATATT TGTAAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAACATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACGGTGAC AGAAAATATG 
TCATTGACTG ATGCGAAGAC GTTGATAGAA AGGTCTAAAG GCAAGTTGAA AATGGTAGTC 
CAGAGAGATG AACGGGCGAC CTTGCTGAAC GTCCCTGACC TTTCTGACAG CATCCATTCT 
GCTAATGCCT CCGAGAGAGA TGACATTTCA GAAATCCAGT CACTGGCATC AGATCATTCT 
GGCCGGTCAC ATGACAGGCC TCCACGCCGC AGCAGCCGGT CCCGCTCTCC TGACCAGCGG 
TCTGAGCCTT CCGACCACTC CAGACAGTCT CCGCAGCAGC CCAGCAATGG CAGTCTCCGG 
AGCAGAGAAG AAGAGAGAAT TTCTAAGCCT GGGGCTGTCT CGACTCCTGT AAAGCATGGA 
GATGACCACA CACCTAAAAC AGTGGAAGAA ATTACTGTTG AGAGAAATGA GAAGCAAACA 
CCTGCCCTTC CAGAACCAAA GCCTGTGTAC GCTCAAGTAG GACAACCAGA CGTGGATTTA 
CCTGTCAGCC CATCTGATGG TGTCCTGCCT AACTCAGCGC ACGAGGACGG GATGCTTCGG 
CCCAGCATGA AATTGGTAAA ATTCAGAAAA GGAGATAGCG TGGGTCTGCG GCTGGCTGGT 
GGAAATGATG TCGGAATATT TGTGGCTGGC GTTCTGGAAG ATAGCCCTGC TGCTAAAGAA 
GGCTTAGAGG AGGGTGATCA GATTCTCAGG GTAAACAATG TGGATTTCAC CAATATCATT 
AGAGAAGAAG CTGTCCTTTT CCTGCTTGAC CTCCCTAAAG GAGAAGAAGT GACCATCCTG 
GCCCAGAAGA AGAAGGATGT TTATCGTCGC ATTGTAGAAT CAGATGTTGG AGATTCTTTC 
TATATTAGAA CCCATTTTGA ATACGAAAAG GAATCTCCCT ATGGACTTAG TTTTAACAAG 
GGAGAGGTAT TTCGTGTTGT GGATACCTTG TACAATGGAA AACTGGGCTC TTGGCTTGCT 
ATTCGAATTG GCAAAAATCA TAAGGAGGTG GAACGAGGCA TCATCCCTAA TAAGAACAGA 
GCTGAACAGC TGGCCAGTGT GCAGTATACA CTTCCCAAAA CTGCGGGGGG CGATCGTGCG 
GACTTCTGGA GGTTCCGGGG TCTCCGCAGC TCCAAGAGGA ATCTTCGGAA AAGCAGAGAG 
GATTTGTCAG CCCAGCCAGT TCAGACGAAA TTTCCAGCTT ATGAAAGGGT TGTTCTTCGA 
GAAGCTGGAT TCCTGAGGCC TGTAACCATC TTTGGACCCA TAGCCGATGT TGCCAGAGAA 
AAGCTGGCCA GAGAAGAGCC AGATATTTAT CAGATTGCAA AAAGTGAACC GCGAGATGCT 
GGAACTGACC AACGTAGCTC TGGCATTATT CGACTTCATA CAATAAAGCA AATAATAGAT 
CAAGACAAGC ATGCCTTATT GGACGTAACA CCAAATGCAG TAGATCGTCT TAATTATGCC 
CAGTGGTATC CAATTGTTGT TTTCCTTAAC CCTGATTCTA AGCAAGGTGT GAAAACAATG 
AGAATGAGGT TATGTCCAGA ATCTCGGAAA AGTGCCAGGA AGTTATATGA GCGCTCTCAC 
AAACTCCGTA AAAACAATCA CCATCTTTTC ACAACTACAA TTAACTTAAA TTCAATGAAC 
GATGGCTGGT ATGGTGCGTT GAAGGAAGCA ATCCAGCAAC AGCAGAACCA GCTGGTATGG 
GTTTCTGAGG GAAAGGCGGA TGGTGCTACA AGTGATGACC TTGATTTGCA TGATGATCGT 
CTGTCCTACC TGTCAGCCCC AGGTAGTGAA TACTCAATGT ATAGCACGGA CAGTAGACAC 
ACTTCTGACT ATGAAGACAC AGATACAGAA GGCGGGGCCT ACACTGATCA AGAACTAGAT 
GAAACTCTTA ATGATGAGGT TGGGACTCCA CCGGAGTCTG CCATTACACG GTCCTCTGAG 
CCTGTAAGAG AGGACTCCTC TGGGATGCAT CATGAAAACC AAACATATCC TCCCTACTCA 
CCACAAGCGC AGCCACAGCC AGTTCCTAGA ATAGACTCCC CGGGATTTAA GACAGCCTCT 
CAACAGGTCT ATAGAAAGGA TCCGTATCCT GAGGAAGTGA TGAGACAGAA TCCAGCTTTG 
AAACAGCCAG CTGTTGGTCA CCCAGGGCAG AGGCCTGAGA AAGAGCCCAG TCTGACCTAT 
GAGCCCCAGC TTCCCTACAT GGAGAAACAA GCCAGCAGAG ACCTCGAGCA GCCCACATAC 
AGATACGAGT CCTCCGGCTA CACAGACCCG TTTTCTCGAA ACTATGACCA TCGCCTGCGA 
TACGAGGACC GCATGCCTGC ATATGAAGAA CAGTGGTCGT ACTACGATGA CAAACAGCCC 
TACCAGCCTC GGCCGCCTTT CGATAGTCAG CACCCTCGAG ACCTTGACCC TAGACAGCAC 
CCTGACGAGC CCTCAGAACG AGGCTATTTC CCACGTTTTG AAGAGCCAGC CCCCCTGTCT 
TATGACAGTC GGCCACGCTA CGAACAGCCG CCCAGAACCT CCACCCTGCG GCATGAAGAG 
CAGCCAGCTC CGGGCTATGA TGTGCACAAC AGACAGAGAC CAGAAGCGCA GCCCTATCCT 
GCAGCAGCCC CTAAGTCGGC GGAGCCCAAG CAGTACTTTG ACCAGTACGG GCGAAGCTAC 
GAGCAAATCC CGCCTCAAGG GTTTGCCTCC AGAACAGGCC ACTACGAACC TCTCCACAGC 
GCCGCAGTCG TCCCTCCGCT CATACCTTCC TCTCAGCATA AGCCAGAAGT CCTGCCTTCC 
AGCACCAAGC CACTGCCCCC ACCTCCAGCC CTAACCGAGG AGGACGAGGA CCCAGCGATG 
AAACCACAGT CTGTGCTCAC CCGAGTGAAA ATGTTTGAAA ACAAAAGGTC TGCATCCCTG 
GAGAACAAGA AGGAGGTGAA TGACACGGCC AGTTTTAAGC CTCCAGAAGC ATCCAAACTT 
CCAGGTGCTC CCGTCACTGG GCCTAAATCC ACCCCTCAGA GCCAGTTCAG TGAACATGAC 
AAAACTTTGT ACAGGATCCC AGAGCCTCAA AAACCGCAAA TGAAGCCTCC TGAAGATATT 
GTCCGGTCAA ATCATTACGA TCCGGAAGAA GATGAAGAGT ATTACCGAAA GCAGCTCTCG 
TACTTTGACC GGAGAAGTTT CGAGAACAAG CCTGCGGCAC ACATTCCTGC CAGCCATCTC 
TCAGAGCCTG CCAAGCCGGC TCATTCTCAG AACCAACCAA ATTTTTCTAG TTATTCTTCA 
AAGGGGAAAG CTGAAGCTGA TGCTCTGGAC CGATCGTTTG GTGAGAAGCG CTATGAGCCA 
GTGCAGCCCA CCCCTCCCCC TCCCCCACTG CCCTCGCAGT ACTCGCAGGC GGCCCAGCCT 
GGCGCCAGCT CTTCTCTCCA CATGCACTCG AAGGGGATAC ATGGTGAAGG TAATTCAATG 
TCATTGGATT TTCAGAATTC TTTGGTATCC AAACCAGACC CACCTCCATC TCAGAGTAAG 
CCAGCAGCTT TCAGAGCACC GCACCGAGAC GATGCCGCTC AGCCTGCTTT ATATCCCCAG 
AAAAGCTTCC CGGAGAAAGC CCCCGTTAAT GGAGCTGAAC AGACTCAGAA AACCATCACT 
CCAGCCTACA ACCGATTCAC ACCAAAACCA TATACCAGTT CTGCCCGGCC ATTCGAGCGC 
AAATTTGAAA GTCCTAAGTT CAACCACAAT CTTCTGCCAG GTGACACTGT ACATAAACCT 
GACTTGTCTG CAAAAGCCCC AGCTTCTCCA AAAACTCTTA TGAAAGCGCA CAGTTCCACA 
CAGCCTCCTG AGTTTGACAG TGGAGTGGAA ACTTTCTCCA TCCATGCAGA GAAGCCCAAG 
TACCAAGTAA CTAATATCAG CACAGTGCCT AGAGCTGCTC CTGTGGGCCC ATCCGCTGTG 
GAAGAAGATG AAGACGAGGA TGGGCACACT GTGGTGGCCA CAGCCCGAGG CATATTTAAC 
AGCAATGGTG GGGTTTTGAG CTCCATAGAG ACCGGCGTCA GTATAATTAT CCCGCAAGGA 
GCCATTCCCG AAGGAATTGA GCAGGAAATC TATTTCAAGG TCTGCCGGGA CAACAGCATC 
CTCCCACCTT TGGATAAAGA GAAAGGTGAA ACCTTGCTAA GCCCCTTAGT GATGTGCGGG 
CCCCATGGCC TCAAGTTCCT GAAGCCCGTG GAGCTGCGCT TGCCACACTG TGCGTCCATG 
ACTCCTGACG GTTGGTCTTT TGCTCTAAAA TCATCCGACT CCTCGTCGGG TGATCCTAAA 
ACCTGGCAAA ACAAGTGTCT TCCTGGAGAT CCAAATTACC TTGTGGGAGC AAACTGTGTG 
TCTGTCTTGA TTGACCACTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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TJP1 cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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