MYL1 cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYL1
Entrez Gene ID	100009400
Full Name	myosin light chain 1
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008258923.1	XP_008257145.1	myosin light chain 1/3, skeletal muscle isoform isoform X1
NM_001101713.1	NP_001095183.1	myosin light chain 1/3, skeletal muscle isoform

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Abnormal movement of tropomyosin and response of myosin heads and actin during the ATPase cycle caused by the Arg167His, Arg167Gly and Lys168Glu mutations in TPM1 gene.
Borovikov YS, Rysev NA, Chernev AA, Avrova SV, Karpicheva OE, Borys D, Śliwińska M, Moraczewska J
Archives of biochemistry and biophysics606157-66(2016 09)

Amplitude of the actomyosin power stroke depends strongly on the isoform of the myosin essential light chain.
Guhathakurta P, Prochniewicz E, Thomas DD
Proceedings of the National Academy of Sciences of the United States of America112(15)4660-5(2015 Apr)

Does Interaction between the Motor and Regulatory Domains of the Myosin Head Occur during ATPase Cycle? Evidence from Thermal Unfolding Studies on Myosin Subfragment 1.
Logvinova DS, Markov DI, Nikolaeva OP, Sluchanko NN, Ushakov DS, Levitsky DI
PloS one10(9)e0137517(2015)

Comparison of orientation and rotational motion of skeletal muscle cross-bridges containing phosphorylated and dephosphorylated myosin regulatory light chain.
Midde K, Rich R, Marandos P, Fudala R, Li A, Gryczynski I, Borejdo J
The Journal of biological chemistry288(10)7012-23(2013 Mar)

The structural dynamics of actin during active interaction with myosin depends on the isoform of the essential light chain.
Prochniewicz E, Guhathakurta P, Thomas DD
Biochemistry52(9)1622-30(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MYL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOb00276	NM_001101713.1 Latest version!	Oryctolagus cuniculus myosin light chain 1 (MYL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OOb08264	XM_008258923.1 Latest version!	Oryctolagus cuniculus myosin light chain 1 (MYL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYL1 ( NM_001101713.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> NP_001095183.1 Oryctolagus cuniculus myosin light chain 1 (MYL1), mRNA.

Price & Availability↑

CloneID	OOb00276
Clone ID Related Accession (Same CDS sequence)	NM_001101713.1
Accession Version	NM_001101713.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577289600000
Organism	Oryctolagus cuniculus(rabbit)
Product	myosin light chain 1/3, skeletal muscle isoform
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X54041.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X54041.1, GBAX01120585.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03328771 [ECO:0000348] ##Evidence-Data-END##

ATGGCACCAA AGAAAGATGT GAAGAAACCT GCTGCTGCTG CTGCCCCAGC CCCTGCCCCA 
GCACCTGCAC CTGCACCTGC CCCAGCCAAA CCCAAAGAAG AAAAAATTGA CCTCTCTGCC 
ATTAAGATCG AGTTCTCTAA GGAACAGCAG GATGAATTCA AGGAGGCGTT TCTCCTGTAT 
GACAGAACAG GTGATTCCAA GATCACTTTA AGCCAGGTTG GTGATGTCCT TCGGGCTCTG 
GGAACAAATC CCACCAATGC AGAGGTCAAG AAGGTTCTGG GAAACCCCAG CAATGAAGAG 
ATGAATGCCA AGAAGATAGA GTTTGAACAG TTTCTGCCCA TGCTGCAAGC TATTTCCAAC 
AACAAGGACC AGGGCACTTA TGAAGATTTT GTTGAGGGGC TGCGTGTCTT TGACAAGGAA 
GGCAATGGCA CAGTCATGGG TGCTGAACTC CGCCATGTTC TAGCTACCTT GGGTGAAAAG 
ATGAAGGAGG AAGAAGTAGA AGCCCTGATG GCAGGTCAAG AAGACTCCAA TGGCTGCATC 
AACTATGAAG CTTTTGTGAA GCATATCATG TCTATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095183.1
CDS	62..640
Translation	MAPKKDVKKPAAAAAPAPAPAPAPAPAPAKPKEEKIDLSAIKIEFSKEQQDEFKEAFLLYDRTGDSKITLSQVGDVLRALGTNPTNAEVKKVLGNPSNEEMNAKKIEFEQFLPMLQAISNNKDQGTYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMKEEEVEALMAGQEDSNGCINYEAFVKHIMSI

Target ORF information:

RefSeq Version	NM_001101713.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus myosin light chain 1 (MYL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101713.1

ORF Insert Sequence:

ATGGCACCAA AGAAAGATGT GAAGAAACCT GCTGCTGCTG CTGCCCCAGC CCCTGCCCCA 
GCACCTGCAC CTGCACCTGC CCCAGCCAAA CCCAAAGAAG AAAAAATTGA CCTCTCTGCC 
ATTAAGATCG AGTTCTCTAA GGAACAGCAG GATGAATTCA AGGAGGCGTT TCTCCTGTAT 
GACAGAACAG GTGATTCCAA GATCACTTTA AGCCAGGTTG GTGATGTCCT TCGGGCTCTG 
GGAACAAATC CCACCAATGC AGAGGTCAAG AAGGTTCTGG GAAACCCCAG CAATGAAGAG 
ATGAATGCCA AGAAGATAGA GTTTGAACAG TTTCTGCCCA TGCTGCAAGC TATTTCCAAC 
AACAAGGACC AGGGCACTTA TGAAGATTTT GTTGAGGGGC TGCGTGTCTT TGACAAGGAA 
GGCAATGGCA CAGTCATGGG TGCTGAACTC CGCCATGTTC TAGCTACCTT GGGTGAAAAG 
ATGAAGGAGG AAGAAGTAGA AGCCCTGATG GCAGGTCAAG AAGACTCCAA TGGCTGCATC 
AACTATGAAG CTTTTGTGAA GCATATCATG TCTATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYL1 ( XM_008258923.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_008257145.1 Oryctolagus cuniculus myosin light chain 1 (MYL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOb08264
Clone ID Related Accession (Same CDS sequence)	XM_008258923.1
Accession Version	XM_008258923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	myosin light chain 1/3, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013675.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTCA GTGCTGACCA GATTGCTGAA TTCAAGGAGG CGTTTCTCCT GTATGACAGA 
ACAGGTGATT CCAAGATCAC TTTAAGCCAG GTTGGTGATG TCCTTCGGGC TCTGGGAACA 
AATCCCACCA ATGCAGAGGT CAAGAAGGTT CTGGGAAACC CCAGCAATGA AGAGATGAAT 
GCCAAGAAGA TAGAGTTTGA ACAGTTTCTG CCCATGCTGC AAGCTATTTC CAACAACAAG 
GACCAGGGCA CTTATGAAGA TTTTGTTGAG GGGCTGCGTG TCTTTGACAA GGAAGGCAAT 
GGCACAGTCA TGGGTGCTGA ACTCCGCCAT GTTCTAGCTA CCCTGGGTGA AAAGATGAAG 
GAGGAAGAAG TAGAAGCCCT GATGGCAGGT CAAGAAGACT CCAATGGCTG CATCAACTAT 
GAAGCTTTTG TGAAGCATAT CATGTCTATC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008257145.1
CDS	83..535
Translation	MSFSADQIAEFKEAFLLYDRTGDSKITLSQVGDVLRALGTNPTNAEVKKVLGNPSNEEMNAKKIEFEQFLPMLQAISNNKDQGTYEDFVEGLRVFDKEGNGTVMGAELRHVLATLGEKMKEEEVEALMAGQEDSNGCINYEAFVKHIMSI

Target ORF information:

RefSeq Version	XM_008258923.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus myosin light chain 1 (MYL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008258923.1

ORF Insert Sequence:

ATGTCCTTCA GTGCTGACCA GATTGCTGAA TTCAAGGAGG CGTTTCTCCT GTATGACAGA 
ACAGGTGATT CCAAGATCAC TTTAAGCCAG GTTGGTGATG TCCTTCGGGC TCTGGGAACA 
AATCCCACCA ATGCAGAGGT CAAGAAGGTT CTGGGAAACC CCAGCAATGA AGAGATGAAT 
GCCAAGAAGA TAGAGTTTGA ACAGTTTCTG CCCATGCTGC AAGCTATTTC CAACAACAAG 
GACCAGGGCA CTTATGAAGA TTTTGTTGAG GGGCTGCGTG TCTTTGACAA GGAAGGCAAT 
GGCACAGTCA TGGGTGCTGA ACTCCGCCAT GTTCTAGCTA CCCTGGGTGA AAAGATGAAG 
GAGGAAGAAG TAGAAGCCCT GATGGCAGGT CAAGAAGACT CCAATGGCTG CATCAACTAT 
GAAGCTTTTG TGAAGCATAT CATGTCTATC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Abnormal movement of tropomyosin and response of myosin heads and actin during the ATPase cycle caused by the Arg167His, Arg167Gly and Lys168Glu mutations in TPM1 gene.
Archives of biochemistry and biophysics606157-66(2016 09)
Borovikov YS,Rysev NA,Chernev AA,Avrova SV,Karpicheva OE,Borys D,Śliwińska M,Moraczewska J

Amplitude of the actomyosin power stroke depends strongly on the isoform of the myosin essential light chain.
Proceedings of the National Academy of Sciences of the United States of America112(15)4660-5(2015 Apr)
Guhathakurta P,Prochniewicz E,Thomas DD

Does Interaction between the Motor and Regulatory Domains of the Myosin Head Occur during ATPase Cycle? Evidence from Thermal Unfolding Studies on Myosin Subfragment 1.
PloS one10(9)e0137517(2015)
Logvinova DS,Markov DI,Nikolaeva OP,Sluchanko NN,Ushakov DS,Levitsky DI

Comparison of orientation and rotational motion of skeletal muscle cross-bridges containing phosphorylated and dephosphorylated myosin regulatory light chain.
The Journal of biological chemistry288(10)7012-23(2013 Mar)
Midde K,Rich R,Marandos P,Fudala R,Li A,Gryczynski I,Borejdo J

The structural dynamics of actin during active interaction with myosin depends on the isoform of the essential light chain.
Biochemistry52(9)1622-30(2013 Mar)
Prochniewicz E,Guhathakurta P,Thomas DD