LPH cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	LPH
Entrez Gene ID	100009256
Full Name	lactase phlorizin hydrolase
Synonyms	LCT
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001101689.1	NP_001095159.1	lactase-phlorizin hydrolase precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Location of the two catalytic sites in intestinal lactase-phlorizin hydrolase. Comparison with sucrase-isomaltase and with other glycosidases, the membrane anchor of lactase-phlorizin hydrolase.
Wacker H, Keller P, Falchetto R, Legler G, Semenza G
The Journal of biological chemistry267(26)18744-52(1992 Sep)

Complete primary structure of human and rabbit lactase-phlorizin hydrolase: implications for biosynthesis, membrane anchoring and evolution of the enzyme.
Mantei N, Villa M, Enzler T, Wacker H, Boll W, James P, Hunziker W, Semenza G
The EMBO journal7(9)2705-13(1988 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LPH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LPH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOb00094	NM_001101689.1 Latest version!	Oryctolagus cuniculus lactase phlorizin hydrolase (LPH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LPH ( NM_001101689.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> NP_001095159.1 Oryctolagus cuniculus lactase phlorizin hydrolase (LPH), mRNA.

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CloneID	OOb00094
Clone ID Related Accession (Same CDS sequence)	NM_001101689.1
Accession Version	NM_001101689.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1532016000000
Organism	Oryctolagus cuniculus(rabbit)
Product	lactase-phlorizin hydrolase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X07995.1. ##Evidence-Data-START## Transcript exon combination :: X07995.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1876840, SAMEA1876870 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCTCT TTTGGAGTAT AGTCTTTACT GTCCTCCTGA GTTTCTCCTG CCGGGGGTCA 
GACTGGGAAT CTGATAGCAA TTTCATTTCA GCTGCAGGGC CTCTAACCAC TGACCTACTG 
CTCAGCCTGC AGTATCCACA GGGAAACCAG ACTTCTGATT TTGCAGCAGG GGGCAAAGAC 
CTTTATGTTT GTTCCCAGCC ACTGCCCGCT TTCCTGCCAG AGTATTTCAG CAGTCTTCGT 
GCCAGTGAGA TCACCCATTA TAAAGTGTTT CTGTCATGGG CACAGCTCCT CCCAGCAGGA 
CACTCCGGAG ACCCAGATGG GAACGCAGTG CGGTGCTATC GGCAGCTCCT TGAGGCCCTC 
AGGGCTGCGC AGCTTCAGCC CATGGTCGTC CTGCACCACC AGCACCTCCC TGCCAGCAGT 
GCCCTGAGGA GCGCCGTCTT CGCTGACCTC TTCGCTGAGT ATGCCACCTT CGCCTTCCAT 
GCCTTTGGGG ACCTCGTGGG GGTCTGGCTC ACCTTTAGTG ACTTGGAGGC AGCCATAAGG 
GAGCTCCCTC AGCCGGAGTC AAGAGCTTCG CGTCTCCAGC TCCTCACTGA GGCCCACAGA 
AAAGCCTATG AGATTTACCA CCAAAAGTAT GCTGCTCAGG GAGGAAAGGT CTCCGTGGTC 
CTCCAGGCTG AAGAGATCTC GGAGCTGCTG CTAGAATCAT CCACGTCTGC ACTTGCCAAG 
GACTCGATTG ATTTCCTCTC TCTTGACTTG TCTTATGAAT GCCAAAGTGA GATGAGTCTA 
CCAGAGAAGC TGAGTAAATT GCAGACCATT GAGCCAAAAG TGAAAGTTTT CATCTTCACT 
CTGAGACTCC AGGACTGCCC CTCCTCCAGG AAGAGCCCAG CCAGCCTGCT CTTCAGCTTT 
ATTGAAGCCA TAAATAAAGA CCAAGTGCTC ACCCTTGGGT TTGATGTTAA TGCGTTTCTG 
AACTGTTCAT CAACTTCCAA GAAAAGCATA TCTTGCTTTC TGACTGACAG CCTGGCCCTT 
CAGACAGACC ACGAGAGAGC TGCCAGGAAC TCAGCCCCTG TGTCGACCTA CCAGAGAGTC 
TGGGAAATGT TTGCTCACCA GCCCAGGGCA GAAAGGGATG CATTCCTGCA GGATACTTTT 
CCCCAAGGCT TCCTGTGGGG CGTCTCCACG GGAGCCTTTA ACGTGGAAGG AGGCTGGGCT 
GAGGGCGGAA GAGGACCCAG TGTCTGGGAT CAGTTTGGGC ATCTAAAAGC TGCCCAGGGC 
CAGGCGACAC CGGAGGTGGC CAGTGACAGC TACTACAAGT GGGCCTCTGA CGTGGCCCTG 
CTTCGCGGCC TCAGGGCTCA GGTGTACAAG TTCTCCATCT CCTGGTCCCG GATCTTCCCC 
ATGGGGCGTG GGAGCAGCCC TAGCCCCCAG GGCGTCGCCT ACTATAACAA GCTGATCGAC 
AGCCTGCTGG ACTCCCACAT TGAGCCCATG GCCACGCTTT TCCACTGGGA CCTGCCTCAG 
GCCCTACAGG ATGAGGGAGG GTGGCAGAAT GAGAGCGTGG TGGATGCCTT TGTGGACTAC 
GCAGCCTTCT GCTTCTCTGC GTTCGGGAAC CGTGTGAAGC TGTGGGTGAC CTTCCACGAG 
CCATGGGTGA TGAGCTACGC GGGTTATGGC ACCGGCCAAC ACGCACCAGG CATCTCAGAC 
CCAGGGATCG CCTCATTTCA GGTGGCTCAC TTGGTCCTCA AGGCCCACGC CAGGACTTGG 
CACCACTACA ACAGCCACCA TCGCCCACAG CAGCAGGGCC GTGTGGGCAT TGTGCTGAAC 
TCAGACTGGG CAGAACCCCT GTCTCCAGAG CGACCTGAGG ACCTTGCAGC CTCTGAGCGC 
TTCTTGCACT TCATGCTGGG CTGGTTTGCA CACCCCATCT TTGTGGATGG GGACTACCCG 
GCCACCATGA AGGCTCAGAT CCAGCAAAGG AATGAACAGT GCCCTAGTCC TGTCGCACAG 
CTCCCGGAGT TCACCGACAC AGAGAAGCAG CTCTTAAAAG GCTCTGCTGA TTTTCTGGGT 
TTGTCTCATT ACACGTCGCG CCTCATCAGC AAGGCCCCAG AAGACAGCTG CATCCCCAGC 
TATGATACCA TCGGAGGCTT CTCCCAACAC ACGGACCCTG CATGGCCCCA GACTTCGTCT 
CCTTGGATTC GCGTGGTGCC CTGGGGTATA AGGAGACTGT TGCAGTTTGT ATCCTTGGAA 
TACACAAAAG GAAAAGTTCC AATCTACCTG GCTGGGAATG GCATGCCCAT AGGGGAGAGT 
GAAAATCTTC TCAGTGATTC CTTGAGAGTA GACTACTTCA ATCAGTACAT CAATGAGGTG 
CTCAAGGCTA TCAAGGAGGA CTCAGTGGAT GTTCGTTCTT ACATTGCTCG CTCCCTCATG 
GATGGCTTTG AAGGCCCCGC CGGCTACAGC CAGAGATTCG GGCTATACCA TGTTAATTTC 
AACGAGAGCA GCAAGCCCAG AACTCCCAGG AAATCTGCCT TCCTTCTCAC CAGCATCATT 
GAAAAGAATG GTTTCCTCAC CAAGGCAGTA AAACAACCAC TGCCACCTAA CTCAGCACAC 
CTGCCTTCCA AAACCAGAGC CTCGGCCCTC CCATCAGAGG TGCCCTCCAA GGCTAAAGTG 
GTTTGGGAGA AGTTCTCCAA CCAGACCAAG TTTGAAAGAG ACTTGTTCTA TCACGGTACA 
TTCCGGGATG ACTTCCTGTG GGGCGTGTCC TCTTCAGCGT ATCAGATTGA AGGAGCTTGG 
GATGCTGATG GCAAAGGGCC CAGCATTTGG GATAACTTTA CTCACACCCC AGGGAACGGT 
GTGACGGACA ACTCCACAGG AGACATCGCC TGTGACAGCT ACAACCAACT GGATGCTGAT 
CTGAATGTGC TTCGAGCTTT GAAGGTGAAA GCCTATCGGT TCTCTCTCTC CTGGTCTCGC 
ATTTTCCCAA CCGGGACAAA CAGCTCTATC AATAGTCATG GTGTTGATTA TTACAACAGG 
CTGATCGATG GCTTGCTGGC AAGCGATATC TTTCCCATGG TGACACTGTT TCACTGGGAC 
CTGCCCCAGG CCCTCCAGGA TATAGGAGGC TGGGAGAATC CTTCCTTGAT TGATCTGTTT 
GACAGCTATG CAGACTATTG TTTCCAGACA TTTGGGGACA GAGTCAAGTT CTGGATCACC 
TTTAATGAGC CCACGTACTA TTCATGGTGG AGTTACGGCT CAGGGACCTT TCCCCCAAAT 
GTGAATGACC CAGGCTGGGC ACCTTATAGG ATCAGCCATG CCCTCATCAA AGCGCATGCC 
CGAGTCTACC ACACGTATGA TGAGAAATAC AGGCAGAGTC AGAACGGGGT CATCTCACTG 
AGTCTTGTCG CACAATGGGC AGAGCCCAAG TCACCGGATG TCCTAAGAGA CGTGGAAGCA 
GCTGACAGAA AGATGCAGTT CACCCTGGGC TGGTACGCCC ACCCCATTTT TAAAACTGGA 
GACTATCCCG ATGCCATGAA GTGGAAAGTG GGCAACAGGA GTGAACTCCA GCACTTAGCC 
ACATCCCGCC TGCCAAGCTT CACTGAGGAA GAGAAGAGCT ACATTAGGGG TACGGCCGAC 
GTCTTCTGCC TCAACACCTA TTCCTCCAAA ATTGTGCAGC ATAAGACACC CGCGCTCAAC 
CCACCCTCCT ACGAAGATGA CCAGGAATTG GCTGAGGAGG AGGATACCTC ATGGCCGACC 
ACGGCAATGA ACAGAGCTGC GTCCTTTGGA ATGCGGAGAC TCCTGAATTG GATCAAGGAA 
GAATATGGAG ACATCCCCAT TTACATCACA GAAAACGGGG TAGGCCTGAC AAATCCAAGA 
CTGGAGGATA TTGACAGGAT ATTTTACTAC AAAACCTACA TCAACGAAGC TTTGAAAGCC 
TACAGGCTTG ATGGTGTCAA CCTTCGAGGT TATTTTGCCT GGTCTCTGAT GGACAACTTT 
GAGTGGCTTC AAGGCTACAC TATCAAGTTT GGACTGTATC ATGTTGACTT TGAAAATGTG 
AACAGGCCTC GCACAGCAAG AATCTCAGCC AGTTACTACA CAGAACTCAT TACCAACAAT 
GGCATGCCAC TGCCCAGTGA GGATGAGTTC GTGTATGGAC AGTTTCCTGA GGGCTTCGTG 
TGGAGTACAT CTACTGCTGC ATTTCAGATC GAAGGTGCAT GGAGAGCAGA TGGCAAAGGC 
CTTGGTATCT GGGACACGTT TACTCACACA CGGCTGAAGA TTGAGAACGA TGACATCGCA 
GATGTGGCCT GTGACAGTTA CCACAAGATC TCTGAGGACG TGGTCGCCCT GCAGAACCTG 
GCCGTAACCC ACTACCGCTT TTCCATCTCC TGGTCTCGCA TCCTCCCTGA CGGAACCACC 
AACTACATCA ACGAAGCAGG CCTGAACTAC TACGTGCGGC TCATTGATGC GCTTCTGGCT 
GCCAACATCA AGCCCCAGGT GACCATGTAC CACTTCGACC TGCCCCAGGC GCTCCAGGAT 
GTTGGAGGCT GGGAGAATGA GACCATTGTG CAGAGGTTCA AGGAGTATGC AGATGTGCTC 
TTCCAGAGGC TGGGAGACAA AGTGAAGTTT TGGATCACAC TAAATGAACC CTTTGTTGTT 
GCCTACCATG GTTATGGCAC TGGGCTGTAT GCCCCAGGGA TCTACTTTAG GCCTGGCACT 
GCTCCCTACA TTGTTGGCCA CAACTTAATA AAGGCTCACG CTGAGGCCTG GCATCTGTAC 
AATGACGTGT ACCGGGCCAG CCAAGGAGGT GTGATTTCCA TCACCATCAG CAGTGACTGG 
GCTGAGCCCA GGGACCCCTC CAATCAAGAG GACGTGGAGG CAGCCAAGAG ATATGTTCAG 
TTCATGGGAG GCTGGTTTGC ACACCCCATT TTCAAGAACG GGGACTACAA CGAGGTGATG 
AAGACACAGA TCCGTGAGAG GAGCTTGGCC GCAGGACTCA ACGAGTCTCG GCTCCCAGAG 
TTCACAGAGA GTGAGAAGAG GAGGATCAAT GGCACCTATG ACTTTTTTGG GTTCAATCAC 
TATACCACTG TCTTGGCGTA CAACTTCAAC TATCCCTCCA TCATGTCCAC TGTTGATGCA 
GACAGGGGAG TTGCCTCCAT CGTAGATCGC TCTTGGCCAG GTTCTGGCTC TTACTGGCTG 
AAGATGACAC CTTTCGGCTT CAGGAGGATC CTGAACTGGA TAAAGGAGGA GTACAACAAC 
CCTCCGATTT ATGTAACAGA GAACGGGGTG TCCCATCGGG GAGACTCATA CCTCAACGAC 
ACTACCAGGA TCTACTACCT CCGCAGTTAC ATCAATGAGG CCCTCAAAGC TGTGCAGCAG 
GACAAGGTGG ACCTTCGAGG ATACACAGTC TGGACTCTAA TGGACAACTT CGAGTGGTAC 
ACAGGCTTCT CAGATAAGTT TGGTCTGCAT TTTGTGAACT ACAGTGACCC TTCTCTGCCA 
AGGATCCCCA GAGAGTCTGC CAAGTTTTAC GCCTCCATAG TCCGGTGCAA CGGCTTCCCT 
GACCCTGCAG AGGGGCCTCA CCCTTGTCTC CTCCAGCCAG AAGACACTGA CCCCACCATG 
AGCCCCGTGA GCCAGGAGGA AGTCCAGTTC CTGGGGCTGT CGCTCGGCTC GACGGAAGCA 
GAGACGGCTT TGTACGTGCT CTTTTCTCTC ATGCTGCTTG GAGTCTGTGG CTTGGCGTTT 
TTATCATACG CATTGTGCAA GAGCTCCAAG CAAAGGAAAA AACTAAGCCA ACAGGAACTG 
AGCCCCGTTT CTTCATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095159.1
CDS	43..5823
Translation	MELFWSIVFTVLLSFSCRGSDWESDSNFISAAGPLTTDLLLSLQYPQGNQTSDFAAGGKDLYVCSQPLPAFLPEYFSSLRASEITHYKVFLSWAQLLPAGHSGDPDGNAVRCYRQLLEALRAAQLQPMVVLHHQHLPASSALRSAVFADLFAEYATFAFHAFGDLVGVWLTFSDLEAAIRELPQPESRASRLQLLTEAHRKAYEIYHQKYAAQGGKVSVVLQAEEISELLLESSTSALAKDSIDFLSLDLSYECQSEMSLPEKLSKLQTIEPKVKVFIFTLRLQDCPSSRKSPASLLFSFIEAINKDQVLTLGFDVNAFLNCSSTSKKSISCFLTDSLALQTDHERAARNSAPVSTYQRVWEMFAHQPRAERDAFLQDTFPQGFLWGVSTGAFNVEGGWAEGGRGPSVWDQFGHLKAAQGQATPEVASDSYYKWASDVALLRGLRAQVYKFSISWSRIFPMGRGSSPSPQGVAYYNKLIDSLLDSHIEPMATLFHWDLPQALQDEGGWQNESVVDAFVDYAAFCFSAFGNRVKLWVTFHEPWVMSYAGYGTGQHAPGISDPGIASFQVAHLVLKAHARTWHHYNSHHRPQQQGRVGIVLNSDWAEPLSPERPEDLAASERFLHFMLGWFAHPIFVDGDYPATMKAQIQQRNEQCPSPVAQLPEFTDTEKQLLKGSADFLGLSHYTSRLISKAPEDSCIPSYDTIGGFSQHTDPAWPQTSSPWIRVVPWGIRRLLQFVSLEYTKGKVPIYLAGNGMPIGESENLLSDSLRVDYFNQYINEVLKAIKEDSVDVRSYIARSLMDGFEGPAGYSQRFGLYHVNFNESSKPRTPRKSAFLLTSIIEKNGFLTKAVKQPLPPNSAHLPSKTRASALPSEVPSKAKVVWEKFSNQTKFERDLFYHGTFRDDFLWGVSSSAYQIEGAWDADGKGPSIWDNFTHTPGNGVTDNSTGDIACDSYNQLDADLNVLRALKVKAYRFSLSWSRIFPTGTNSSINSHGVDYYNRLIDGLLASDIFPMVTLFHWDLPQALQDIGGWENPSLIDLFDSYADYCFQTFGDRVKFWITFNEPTYYSWWSYGSGTFPPNVNDPGWAPYRISHALIKAHARVYHTYDEKYRQSQNGVISLSLVAQWAEPKSPDVLRDVEAADRKMQFTLGWYAHPIFKTGDYPDAMKWKVGNRSELQHLATSRLPSFTEEEKSYIRGTADVFCLNTYSSKIVQHKTPALNPPSYEDDQELAEEEDTSWPTTAMNRAASFGMRRLLNWIKEEYGDIPIYITENGVGLTNPRLEDIDRIFYYKTYINEALKAYRLDGVNLRGYFAWSLMDNFEWLQGYTIKFGLYHVDFENVNRPRTARISASYYTELITNNGMPLPSEDEFVYGQFPEGFVWSTSTAAFQIEGAWRADGKGLGIWDTFTHTRLKIENDDIADVACDSYHKISEDVVALQNLAVTHYRFSISWSRILPDGTTNYINEAGLNYYVRLIDALLAANIKPQVTMYHFDLPQALQDVGGWENETIVQRFKEYADVLFQRLGDKVKFWITLNEPFVVAYHGYGTGLYAPGIYFRPGTAPYIVGHNLIKAHAEAWHLYNDVYRASQGGVISITISSDWAEPRDPSNQEDVEAAKRYVQFMGGWFAHPIFKNGDYNEVMKTQIRERSLAAGLNESRLPEFTESEKRRINGTYDFFGFNHYTTVLAYNFNYPSIMSTVDADRGVASIVDRSWPGSGSYWLKMTPFGFRRILNWIKEEYNNPPIYVTENGVSHRGDSYLNDTTRIYYLRSYINEALKAVQQDKVDLRGYTVWTLMDNFEWYTGFSDKFGLHFVNYSDPSLPRIPRESAKFYASIVRCNGFPDPAEGPHPCLLQPEDTDPTMSPVSQEEVQFLGLSLGSTEAETALYVLFSLMLLGVCGLAFLSYALCKSSKQRKKLSQQELSPVSSF

Target ORF information:

RefSeq Version	NM_001101689.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus lactase phlorizin hydrolase (LPH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101689.1

ORF Insert Sequence:

ATGGAGCTCT TTTGGAGTAT AGTCTTTACT GTCCTCCTGA GTTTCTCCTG CCGGGGGTCA 
GACTGGGAAT CTGATAGCAA TTTCATTTCA GCTGCAGGGC CTCTAACCAC TGACCTACTG 
CTCAGCCTGC AGTATCCACA GGGAAACCAG ACTTCTGATT TTGCAGCAGG GGGCAAAGAC 
CTTTATGTTT GTTCCCAGCC ACTGCCCGCT TTCCTGCCAG AGTATTTCAG CAGTCTTCGT 
GCCAGTGAGA TCACCCATTA TAAAGTGTTT CTGTCATGGG CACAGCTCCT CCCAGCAGGA 
CACTCCGGAG ACCCAGATGG GAACGCAGTG CGGTGCTATC GGCAGCTCCT TGAGGCCCTC 
AGGGCTGCGC AGCTTCAGCC CATGGTCGTC CTGCACCACC AGCACCTCCC TGCCAGCAGT 
GCCCTGAGGA GCGCCGTCTT CGCTGACCTC TTCGCTGAGT ATGCCACCTT CGCCTTCCAT 
GCCTTTGGGG ACCTCGTGGG GGTCTGGCTC ACCTTTAGTG ACTTGGAGGC AGCCATAAGG 
GAGCTCCCTC AGCCGGAGTC AAGAGCTTCG CGTCTCCAGC TCCTCACTGA GGCCCACAGA 
AAAGCCTATG AGATTTACCA CCAAAAGTAT GCTGCTCAGG GAGGAAAGGT CTCCGTGGTC 
CTCCAGGCTG AAGAGATCTC GGAGCTGCTG CTAGAATCAT CCACGTCTGC ACTTGCCAAG 
GACTCGATTG ATTTCCTCTC TCTTGACTTG TCTTATGAAT GCCAAAGTGA GATGAGTCTA 
CCAGAGAAGC TGAGTAAATT GCAGACCATT GAGCCAAAAG TGAAAGTTTT CATCTTCACT 
CTGAGACTCC AGGACTGCCC CTCCTCCAGG AAGAGCCCAG CCAGCCTGCT CTTCAGCTTT 
ATTGAAGCCA TAAATAAAGA CCAAGTGCTC ACCCTTGGGT TTGATGTTAA TGCGTTTCTG 
AACTGTTCAT CAACTTCCAA GAAAAGCATA TCTTGCTTTC TGACTGACAG CCTGGCCCTT 
CAGACAGACC ACGAGAGAGC TGCCAGGAAC TCAGCCCCTG TGTCGACCTA CCAGAGAGTC 
TGGGAAATGT TTGCTCACCA GCCCAGGGCA GAAAGGGATG CATTCCTGCA GGATACTTTT 
CCCCAAGGCT TCCTGTGGGG CGTCTCCACG GGAGCCTTTA ACGTGGAAGG AGGCTGGGCT 
GAGGGCGGAA GAGGACCCAG TGTCTGGGAT CAGTTTGGGC ATCTAAAAGC TGCCCAGGGC 
CAGGCGACAC CGGAGGTGGC CAGTGACAGC TACTACAAGT GGGCCTCTGA CGTGGCCCTG 
CTTCGCGGCC TCAGGGCTCA GGTGTACAAG TTCTCCATCT CCTGGTCCCG GATCTTCCCC 
ATGGGGCGTG GGAGCAGCCC TAGCCCCCAG GGCGTCGCCT ACTATAACAA GCTGATCGAC 
AGCCTGCTGG ACTCCCACAT TGAGCCCATG GCCACGCTTT TCCACTGGGA CCTGCCTCAG 
GCCCTACAGG ATGAGGGAGG GTGGCAGAAT GAGAGCGTGG TGGATGCCTT TGTGGACTAC 
GCAGCCTTCT GCTTCTCTGC GTTCGGGAAC CGTGTGAAGC TGTGGGTGAC CTTCCACGAG 
CCATGGGTGA TGAGCTACGC GGGTTATGGC ACCGGCCAAC ACGCACCAGG CATCTCAGAC 
CCAGGGATCG CCTCATTTCA GGTGGCTCAC TTGGTCCTCA AGGCCCACGC CAGGACTTGG 
CACCACTACA ACAGCCACCA TCGCCCACAG CAGCAGGGCC GTGTGGGCAT TGTGCTGAAC 
TCAGACTGGG CAGAACCCCT GTCTCCAGAG CGACCTGAGG ACCTTGCAGC CTCTGAGCGC 
TTCTTGCACT TCATGCTGGG CTGGTTTGCA CACCCCATCT TTGTGGATGG GGACTACCCG 
GCCACCATGA AGGCTCAGAT CCAGCAAAGG AATGAACAGT GCCCTAGTCC TGTCGCACAG 
CTCCCGGAGT TCACCGACAC AGAGAAGCAG CTCTTAAAAG GCTCTGCTGA TTTTCTGGGT 
TTGTCTCATT ACACGTCGCG CCTCATCAGC AAGGCCCCAG AAGACAGCTG CATCCCCAGC 
TATGATACCA TCGGAGGCTT CTCCCAACAC ACGGACCCTG CATGGCCCCA GACTTCGTCT 
CCTTGGATTC GCGTGGTGCC CTGGGGTATA AGGAGACTGT TGCAGTTTGT ATCCTTGGAA 
TACACAAAAG GAAAAGTTCC AATCTACCTG GCTGGGAATG GCATGCCCAT AGGGGAGAGT 
GAAAATCTTC TCAGTGATTC CTTGAGAGTA GACTACTTCA ATCAGTACAT CAATGAGGTG 
CTCAAGGCTA TCAAGGAGGA CTCAGTGGAT GTTCGTTCTT ACATTGCTCG CTCCCTCATG 
GATGGCTTTG AAGGCCCCGC CGGCTACAGC CAGAGATTCG GGCTATACCA TGTTAATTTC 
AACGAGAGCA GCAAGCCCAG AACTCCCAGG AAATCTGCCT TCCTTCTCAC CAGCATCATT 
GAAAAGAATG GTTTCCTCAC CAAGGCAGTA AAACAACCAC TGCCACCTAA CTCAGCACAC 
CTGCCTTCCA AAACCAGAGC CTCGGCCCTC CCATCAGAGG TGCCCTCCAA GGCTAAAGTG 
GTTTGGGAGA AGTTCTCCAA CCAGACCAAG TTTGAAAGAG ACTTGTTCTA TCACGGTACA 
TTCCGGGATG ACTTCCTGTG GGGCGTGTCC TCTTCAGCGT ATCAGATTGA AGGAGCTTGG 
GATGCTGATG GCAAAGGGCC CAGCATTTGG GATAACTTTA CTCACACCCC AGGGAACGGT 
GTGACGGACA ACTCCACAGG AGACATCGCC TGTGACAGCT ACAACCAACT GGATGCTGAT 
CTGAATGTGC TTCGAGCTTT GAAGGTGAAA GCCTATCGGT TCTCTCTCTC CTGGTCTCGC 
ATTTTCCCAA CCGGGACAAA CAGCTCTATC AATAGTCATG GTGTTGATTA TTACAACAGG 
CTGATCGATG GCTTGCTGGC AAGCGATATC TTTCCCATGG TGACACTGTT TCACTGGGAC 
CTGCCCCAGG CCCTCCAGGA TATAGGAGGC TGGGAGAATC CTTCCTTGAT TGATCTGTTT 
GACAGCTATG CAGACTATTG TTTCCAGACA TTTGGGGACA GAGTCAAGTT CTGGATCACC 
TTTAATGAGC CCACGTACTA TTCATGGTGG AGTTACGGCT CAGGGACCTT TCCCCCAAAT 
GTGAATGACC CAGGCTGGGC ACCTTATAGG ATCAGCCATG CCCTCATCAA AGCGCATGCC 
CGAGTCTACC ACACGTATGA TGAGAAATAC AGGCAGAGTC AGAACGGGGT CATCTCACTG 
AGTCTTGTCG CACAATGGGC AGAGCCCAAG TCACCGGATG TCCTAAGAGA CGTGGAAGCA 
GCTGACAGAA AGATGCAGTT CACCCTGGGC TGGTACGCCC ACCCCATTTT TAAAACTGGA 
GACTATCCCG ATGCCATGAA GTGGAAAGTG GGCAACAGGA GTGAACTCCA GCACTTAGCC 
ACATCCCGCC TGCCAAGCTT CACTGAGGAA GAGAAGAGCT ACATTAGGGG TACGGCCGAC 
GTCTTCTGCC TCAACACCTA TTCCTCCAAA ATTGTGCAGC ATAAGACACC CGCGCTCAAC 
CCACCCTCCT ACGAAGATGA CCAGGAATTG GCTGAGGAGG AGGATACCTC ATGGCCGACC 
ACGGCAATGA ACAGAGCTGC GTCCTTTGGA ATGCGGAGAC TCCTGAATTG GATCAAGGAA 
GAATATGGAG ACATCCCCAT TTACATCACA GAAAACGGGG TAGGCCTGAC AAATCCAAGA 
CTGGAGGATA TTGACAGGAT ATTTTACTAC AAAACCTACA TCAACGAAGC TTTGAAAGCC 
TACAGGCTTG ATGGTGTCAA CCTTCGAGGT TATTTTGCCT GGTCTCTGAT GGACAACTTT 
GAGTGGCTTC AAGGCTACAC TATCAAGTTT GGACTGTATC ATGTTGACTT TGAAAATGTG 
AACAGGCCTC GCACAGCAAG AATCTCAGCC AGTTACTACA CAGAACTCAT TACCAACAAT 
GGCATGCCAC TGCCCAGTGA GGATGAGTTC GTGTATGGAC AGTTTCCTGA GGGCTTCGTG 
TGGAGTACAT CTACTGCTGC ATTTCAGATC GAAGGTGCAT GGAGAGCAGA TGGCAAAGGC 
CTTGGTATCT GGGACACGTT TACTCACACA CGGCTGAAGA TTGAGAACGA TGACATCGCA 
GATGTGGCCT GTGACAGTTA CCACAAGATC TCTGAGGACG TGGTCGCCCT GCAGAACCTG 
GCCGTAACCC ACTACCGCTT TTCCATCTCC TGGTCTCGCA TCCTCCCTGA CGGAACCACC 
AACTACATCA ACGAAGCAGG CCTGAACTAC TACGTGCGGC TCATTGATGC GCTTCTGGCT 
GCCAACATCA AGCCCCAGGT GACCATGTAC CACTTCGACC TGCCCCAGGC GCTCCAGGAT 
GTTGGAGGCT GGGAGAATGA GACCATTGTG CAGAGGTTCA AGGAGTATGC AGATGTGCTC 
TTCCAGAGGC TGGGAGACAA AGTGAAGTTT TGGATCACAC TAAATGAACC CTTTGTTGTT 
GCCTACCATG GTTATGGCAC TGGGCTGTAT GCCCCAGGGA TCTACTTTAG GCCTGGCACT 
GCTCCCTACA TTGTTGGCCA CAACTTAATA AAGGCTCACG CTGAGGCCTG GCATCTGTAC 
AATGACGTGT ACCGGGCCAG CCAAGGAGGT GTGATTTCCA TCACCATCAG CAGTGACTGG 
GCTGAGCCCA GGGACCCCTC CAATCAAGAG GACGTGGAGG CAGCCAAGAG ATATGTTCAG 
TTCATGGGAG GCTGGTTTGC ACACCCCATT TTCAAGAACG GGGACTACAA CGAGGTGATG 
AAGACACAGA TCCGTGAGAG GAGCTTGGCC GCAGGACTCA ACGAGTCTCG GCTCCCAGAG 
TTCACAGAGA GTGAGAAGAG GAGGATCAAT GGCACCTATG ACTTTTTTGG GTTCAATCAC 
TATACCACTG TCTTGGCGTA CAACTTCAAC TATCCCTCCA TCATGTCCAC TGTTGATGCA 
GACAGGGGAG TTGCCTCCAT CGTAGATCGC TCTTGGCCAG GTTCTGGCTC TTACTGGCTG 
AAGATGACAC CTTTCGGCTT CAGGAGGATC CTGAACTGGA TAAAGGAGGA GTACAACAAC 
CCTCCGATTT ATGTAACAGA GAACGGGGTG TCCCATCGGG GAGACTCATA CCTCAACGAC 
ACTACCAGGA TCTACTACCT CCGCAGTTAC ATCAATGAGG CCCTCAAAGC TGTGCAGCAG 
GACAAGGTGG ACCTTCGAGG ATACACAGTC TGGACTCTAA TGGACAACTT CGAGTGGTAC 
ACAGGCTTCT CAGATAAGTT TGGTCTGCAT TTTGTGAACT ACAGTGACCC TTCTCTGCCA 
AGGATCCCCA GAGAGTCTGC CAAGTTTTAC GCCTCCATAG TCCGGTGCAA CGGCTTCCCT 
GACCCTGCAG AGGGGCCTCA CCCTTGTCTC CTCCAGCCAG AAGACACTGA CCCCACCATG 
AGCCCCGTGA GCCAGGAGGA AGTCCAGTTC CTGGGGCTGT CGCTCGGCTC GACGGAAGCA 
GAGACGGCTT TGTACGTGCT CTTTTCTCTC ATGCTGCTTG GAGTCTGTGG CTTGGCGTTT 
TTATCATACG CATTGTGCAA GAGCTCCAAG CAAAGGAAAA AACTAAGCCA ACAGGAACTG 
AGCCCCGTTT CTTCATTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Location of the two catalytic sites in intestinal lactase-phlorizin hydrolase. Comparison with sucrase-isomaltase and with other glycosidases, the membrane anchor of lactase-phlorizin hydrolase.
The Journal of biological chemistry267(26)18744-52(1992 Sep)
Wacker H,Keller P,Falchetto R,Legler G,Semenza G

Complete primary structure of human and rabbit lactase-phlorizin hydrolase: implications for biosynthesis, membrane anchoring and evolution of the enzyme.
The EMBO journal7(9)2705-13(1988 Sep)
Mantei N,Villa M,Enzler T,Wacker H,Boll W,James P,Hunziker W,Semenza G

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LPH cDNA ORF clone, Oryctolagus cuniculus(rabbit)

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