SI cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI
Entrez Gene ID	100009093
Full Name	sucrase-isomaltase
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001082266.1	NP_001075735.1	sucrase-isomaltase, intestinal

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The sucrase-isomaltase complex: primary structure, membrane-orientation, and evolution of a stalked, intrinsic brush border protein.
Hunziker W, Spiess M, Semenza G, Lodish HF
Cell46(2)227-34(1986 Jul)

N-Terminal sequences of pig intestinal sucrase-isomaltase and pro-sucrase--isomaltase. Implications for the biosynthesis and membrane insertion of pro-sucrase--isomaltase.
Sjöström H, Norén O, Christiansen LA, Wacker H, Spiess M, Bigler-Meier B, Rickli EE, Semenza G
FEBS letters148(2)321-5(1982 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOb00101	NM_001082266.1 Latest version!	Oryctolagus cuniculus sucrase-isomaltase (SI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI ( NM_001082266.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> NP_001075735.1 Oryctolagus cuniculus sucrase-isomaltase (SI), mRNA.

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CloneID	OOb00101
Clone ID Related Accession (Same CDS sequence)	NM_001082266.1
Accession Version	NM_001082266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550246400000
Organism	Oryctolagus cuniculus(rabbit)
Product	sucrase-isomaltase, intestinal
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M14046.1. ##Evidence-Data-START## Transcript exon combination :: M14046.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03328699 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAAGA GGAAATTTTC TGGCTTAGAA ATCACTCTGA TTGTCCTCTT TGTGATAGTT 
TTTATAATAG CTATTGCTCT AATTGCTGTG TTAGCAACTA AGACACCAGC AGTTGAAGAG 
GTTAATCCTT CCAGTTCAAC TCCAACTACG ACTTCAACTA CAACTTCAAC TTCCGGTTCA 
GTAAGCTGTC CCAGTGAGCT AAATGAGGTT GTCAATGAGA GAATAAACTG CATTCCAGAA 
CAATCTCCAA CACAGGCGAT TTGTGCACAA CGAAACTGCT GTTGGAGACC ATGGAACAAC 
TCAGATATCC CATGGTGCTT CTTTGTAGAC AATCATGGCT ACAATGTTGA AGGAATGACA 
ACAACAAGTA CTGGACTTGA AGCCAGGTTA AACAGGAAAT CAACACCTAC ACTTTTTGGA 
AATGATATTA ACAATGTCCT CCTCACCACT GAAAGTCAGA CAGCCAATCG TTTACGGTTC 
AAGCTTACTG ATCCAAATAA TAAAAGATAT GAAGTTCCTC ATCAATTTGT GACAGAATTT 
GCTGGACCTG CAGCTACTGA GACATTATAT GATGTACAGG TTACAGAAAA TCCTTTTAGC 
ATCAAAGTTA TCAGGAAAAG TAACAACAGG ATTTTGTTTG ACAGCAGCAT TGGTCCCCTG 
GTGTACTCTG ACCAGTATTT ACAGATCTCA ACCAGACTTC CCAGTGAGTA CATGTATGGT 
TTTGGAGAAC ATGTTCACAA GAGATTTCGT CATGATTTAT ATTGGAAAAC ATGGCCGATC 
TTTACTCGGG ATCAACATAC TGATGATAAT AATAATAATT TATATGGCCA TCAGACATTC 
TTCATGTGTA TTGAAGACAC TACTGGAAAG TCATTTGGTG TTTTTTTAAT GAACAGCAAT 
GCAATGGAAA TTTTCATCCA GCCTACTCCA ATAGTAACCT ATAGAGTTAT CGGTGGCATC 
CTAGACTTTT ACATCTTTTT AGGAGATACA CCAGAACAGG TGGTCCAACA GTATCAAGAG 
CTCATTGGAC GACCAGCAAT GCCAGCATAC TGGAGTCTTG GATTCCAACT GAGTCGCTGG 
AATTACAATT CTCTAGATGT AGTCAAAGAG GTGGTAAGAA GAAACAGGGA AGCTCTCATA 
CCATTTGATA CACAAGTCAG TGATATTGAC TATATGGAAG ACAAGAAAGA CTTCACTTAT 
GATAGAGTGG CATATAATGG GCTCCCTGAC TTTGTTCAAG ATTTGCATGA CCATGGACAG 
AAATATGTTA TTATCTTGGA CCCTGCAATT TCTATAAATA GGCGTGCCAG TGGAGAAGCA 
TATGAATCCT ATGACAGAGG AAATGCACAA AATGTGTGGG TAAATGAGTC AGATGGAACT 
ACACCAATAG TTGGAGAGGT CTGGCCAGGA GATACAGTAT ATCCTGATTT CACCAGTCCA 
AACTGCATAG AATGGTGGGC AAATGAATGC AATATTTTCC ATCAAGAAGT GAATTATGAT 
GGACTTTGGA TTGACATGAA TGAAGTGTCC AGCTTTGTTC AAGGCTCAAA TAAGGGCTGT 
AATGACAACA CATTGAATTA TCCACCTTAC ATTCCTGATA TTGTTGATAA GCTCATGTAT 
TCCAAAACAC TTTGCATGGA TTCTGTGCAG TACTGGGGAA AACAGTATGA TGTTCACAGC 
CTATATGGAT ATAGCATGGC TATAGCCACT GAAAGAGCTG TAGAAAGAGT TTTCCCTAAC 
AAGAGAAGCT TCATCCTTAC TCGGTCAACT TTTGCTGGAA GTGGAAGACA TGCTGCACAT 
TGGCTAGGAG ATAACACGGC TACTTGGGAA CAAATGGAGT GGTCTATCAC TGGGATGCTA 
GAGTTCGGTT TGTTTGGAAT GCCTTTGGTT GGTGCAGATA TCTGTGGATT TCTGGCTGAA 
ACCACAGAAG AGCTTTGCAG AAGGTGGATG CAACTTGGGG CCTTTTATCC GTTTTCCAGA 
AATCATAATG CTGATGGATT TGAGCATCAG GATCCCGCAT TTTTTGGACA AGACTCACTT 
TTGGTTAAGT CATCTCGGCA CTACTTAAAT ATCCGCTATA CCTTATTGCC TTTCCTCTAT 
ACTCTATTTT ACAAAGCCCA TGCGTTTGGA GAAACAGTAG CAAGGCCAGT TCTTCATGAG 
TTTTATGAGG ACACAAACAG CTGGGTTGAG GACAGAGAGT TTTTATGGGG CCCCGCTTTA 
CTCATTACTC CTGTTTTGAC ACAGGGAGCA GAGACAGTGA GTGCATACAT TCCTGATGCT 
GTTTGGTATG ATTATGAAAC AGGTGCAAAA AGGCCATGGA GAAAGCAACG GGTGGAAATG 
AGTCTTCCAG CAGACAAAAT AGGACTGCAT CTTAGAGGAG GTTACATTAT CCCCATTCAA 
CAACCTGCTG TAACAACAAC AGCAAGTCGT ATGAATCCTC TAGGACTTAT AATTGCATTA 
AATGATGATA ACACAGCAGT AGGAGACTTT TTCTGGGATG ATGGAGAAAC TAAAGATACC 
GTACAAAATG ACAACTACAT ATTATATACA TTTGCAGTTT CTAACAACAA TTTAAATATC 
ACATGCACAC ATGAATTATA CTCAGAAGGA ACAACTTTAG CATTTCAGAC TATAAAAATT 
CTCGGGGTGA CGGAGACCGT TACACAGGTT ACAGTGGCAG AAAATAATCA GTCAATGAGT 
ACGCACTCTA ATTTCACATA TGATCCCTCC AACCAGGTTC TCTTAATTGA GAATCTCAAC 
TTTAACCTTG GAAGAAACTT CAGAGTTCAA TGGGATCAAA CTTTCTTAGA AAGCGAAAAG 
ATTACTTGTT ATCCAGATGC AGACATTGCA ACTCAGGAAA AGTGCACTCA ACGTGGATGT 
ATATGGGACA CGAATACTGT GAACCCTCGA GCACCTGAAT GTTACTTCCC TAAAACTGAC 
AACCCTTATT CAGTTAGCTC AACTCAGTAT TCACCGACGG GTATAACAGC TGACCTCCAG 
TTGAATCCTA CAAGAACCAG AATAACTTTA CCTTCTGAAC CCATTACAAA TCTTCGTGTG 
GAGGTGAAAT ATCACAAAAA TGATATGGTA CAGTTTAAGA TTTTTGATCC CCAAAACAAG 
AGATATGAAG TTCCGGTACC ACTAGACATT CCAGCCACTC CAACAAGCAC ACAGGAAAAC 
AGACTTTATG ATGTTGAAAT CAAGGAAAAC CCTTTTGGTA TCCAGATTCG ACGGAGAAGC 
ACTGGAAAAG TTATTTGGGA TTCTTGTCTA CCTGGTTTTG CTTTTAATGA TCAGTTCATT 
CAAATATCTA CTCGCCTGCC ATCGGAATAT ATATATGGTT TTGGAGAAGC AGAACACACA 
GCATTTAAGA GAGATCTGAA CTGGCATACT TGGGGGATGT TCACAAGAGA CCAACCCCCT 
GGTTACAAAC TTAATTCCTA TGGATTTCAT CCTTATTACA TGGCTCTGGA AGATGAAGGA 
AATGCCCATG GAGTTTTGTT ACTGAACAGC AATGCAATGG ATGTTACATT CATGCCAACT 
CCAGCTCTAA CTTATCGTGT AATTGGAGGA ATCTTGGATT TTTATATGTT CTTAGGCCCA 
ACTCCAGAAG TTGCAACCCA ACAATATCAT GAAGTAATTG GCCATCCAGT CATGCCACCG 
TATTGGTCTT TAGGATTCCA ATTATGTCGG TATGGATACA GAAATACTTC AGAGATTATT 
GAACTATATG AGGGGATGGT GGCTGCTGAC ATACCCTATG ATGTTCAATA CACAGATATT 
GATTACATGG AAAGGCAACT AGACTTTACA ATTGATGAAA ACTTTCGTGA ACTTCCTCAA 
TTTGTTGATA GAATAAGAGG AGAAGGAATG AGATACATTA TTATTCTGGA CCCAGCAATT 
TCAGGAAATG AAACACGGCC TTACCCTGCA TTTGACAGAG GGGAAGCTAA AGATGTCTTC 
GTCAAATGGC CTAATACCAG TGATATTTGT TGGGCAAAGG TTTGGCCAGA CTTGCCTAAT 
ATAACAATAG ATGAAAGTCT AACTGAAGAT GAAGCTGTTA ACGCTTCCCG AGCTCACGCA 
GCTTTTCCAG ATTTCTTCAG GAATTCCACA GCAGAGTGGT GGACAAGAGA AATTTTAGAT 
TTCTACAATA ACTATATGAA GTTTGATGGC TTGTGGATTG ATATGAATGA ACCATCAAGT 
TTTGTGAATG GAACAACTAC TAATGTATGC AGAAATACAG AGCTAAATTA TCCACCTTAT 
TTCCCAGAAC TTACAAAACG GACAGATGGA TTGCATTTCA GAACTATGTG CATGGAAACT 
GAGCATATTC TTAGTGATGG ATCATCAGTT TTACATTATG ATGTTCACAA TCTCTATGGA 
TGGTCACAAG CAAAGCCTAC TTATGATGCC TTGCAGAAGA CAACTGGCAA AAGAGGGATA 
GTTATTTCTC GTTCTACATA TCCTACTGCT GGGCGATGGG CAGGACACTG GCTTGGAGAC 
AACTATGCAC GGTGGGACAA TATGGACAAA TCGATCATTG GTATGATGGA ATTTAGTCTC 
TTTGGAATAT CATATACTGG AGCAGACATC TGTGGTTTCT TCAATGATTC AGAATATCAT 
CTCTGTACTC GCTGGACACA ACTTGGAGCA TTTTATCCAT TTGCAAGAAA TCACAACATT 
CAATTTACTA GAAGACAAGA CCCTGTTTCA TGGAATCAAA CTTTTGTTGA AATGACAAGG 
AATGTTCTGA ATATTAGATA TACTTTATTA CCTTATTTCT ATACACAATT GCATGAAATT 
CATGCTCATG GTGGCACTGT TATCCGACCC CTTATGCATG AGTTCTTTGA TGACAGAACA 
ACTTGGGACA TATTTCTACA GTTTCTGTGG GGTCCAGCAT TTATGGTTAC CCCTGTGTTG 
GAACCTTATA CTACTGTTGT ACGTGGCTAT GTCCCTAATG CTCGGTGGTT TGACTATCAT 
ACGGGAGAAG ATATTGGAAT CAGAGGACAA GTTCAAGATT TAACACTCCT TATGAATGCA 
ATAAATCTAC ATGTTCGTGG TGGTCACATC CTACCATGTC AGGAACCTGC TCGAACCACA 
TTTTTAAGTC GACAGAAGTA CATGAAGCTC ATTGTTGCTG CAGATGATAA TCACATGGCA 
CAAGGATCTC TCTTCTGGGA TGATGGGGAC ACTATAGATA CCTATGAGAG AGACCTGTAT 
TTATCAGTAC AATTTAATTT AAATAAGACA ACTTTGACAA GCACGCTATT GAAAACTGGT 
TACATAAATA AAACTGAAAT AAGACTTGGA TATGTCCATG TATGGGGCAT AGGAAACACT 
TTGATCAATG AAGTTAATTT AATGTATAAT GAAATAAATT ATCCACTTAT ATTTAACCAA 
ACCCAAGCCC AAGAGATATT AAATATTGAT CTGACGGCAC ATGAAGTTAC TCTAGATGAC 
CCAATAGAAA TCTCCTGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075735.1
CDS	27..5510
Translation	MAKRKFSGLEITLIVLFVIVFIIAIALIAVLATKTPAVEEVNPSSSTPTTTSTTTSTSGSVSCPSELNEVVNERINCIPEQSPTQAICAQRNCCWRPWNNSDIPWCFFVDNHGYNVEGMTTTSTGLEARLNRKSTPTLFGNDINNVLLTTESQTANRLRFKLTDPNNKRYEVPHQFVTEFAGPAATETLYDVQVTENPFSIKVIRKSNNRILFDSSIGPLVYSDQYLQISTRLPSEYMYGFGEHVHKRFRHDLYWKTWPIFTRDQHTDDNNNNLYGHQTFFMCIEDTTGKSFGVFLMNSNAMEIFIQPTPIVTYRVIGGILDFYIFLGDTPEQVVQQYQELIGRPAMPAYWSLGFQLSRWNYNSLDVVKEVVRRNREALIPFDTQVSDIDYMEDKKDFTYDRVAYNGLPDFVQDLHDHGQKYVIILDPAISINRRASGEAYESYDRGNAQNVWVNESDGTTPIVGEVWPGDTVYPDFTSPNCIEWWANECNIFHQEVNYDGLWIDMNEVSSFVQGSNKGCNDNTLNYPPYIPDIVDKLMYSKTLCMDSVQYWGKQYDVHSLYGYSMAIATERAVERVFPNKRSFILTRSTFAGSGRHAAHWLGDNTATWEQMEWSITGMLEFGLFGMPLVGADICGFLAETTEELCRRWMQLGAFYPFSRNHNADGFEHQDPAFFGQDSLLVKSSRHYLNIRYTLLPFLYTLFYKAHAFGETVARPVLHEFYEDTNSWVEDREFLWGPALLITPVLTQGAETVSAYIPDAVWYDYETGAKRPWRKQRVEMSLPADKIGLHLRGGYIIPIQQPAVTTTASRMNPLGLIIALNDDNTAVGDFFWDDGETKDTVQNDNYILYTFAVSNNNLNITCTHELYSEGTTLAFQTIKILGVTETVTQVTVAENNQSMSTHSNFTYDPSNQVLLIENLNFNLGRNFRVQWDQTFLESEKITCYPDADIATQEKCTQRGCIWDTNTVNPRAPECYFPKTDNPYSVSSTQYSPTGITADLQLNPTRTRITLPSEPITNLRVEVKYHKNDMVQFKIFDPQNKRYEVPVPLDIPATPTSTQENRLYDVEIKENPFGIQIRRRSTGKVIWDSCLPGFAFNDQFIQISTRLPSEYIYGFGEAEHTAFKRDLNWHTWGMFTRDQPPGYKLNSYGFHPYYMALEDEGNAHGVLLLNSNAMDVTFMPTPALTYRVIGGILDFYMFLGPTPEVATQQYHEVIGHPVMPPYWSLGFQLCRYGYRNTSEIIELYEGMVAADIPYDVQYTDIDYMERQLDFTIDENFRELPQFVDRIRGEGMRYIIILDPAISGNETRPYPAFDRGEAKDVFVKWPNTSDICWAKVWPDLPNITIDESLTEDEAVNASRAHAAFPDFFRNSTAEWWTREILDFYNNYMKFDGLWIDMNEPSSFVNGTTTNVCRNTELNYPPYFPELTKRTDGLHFRTMCMETEHILSDGSSVLHYDVHNLYGWSQAKPTYDALQKTTGKRGIVISRSTYPTAGRWAGHWLGDNYARWDNMDKSIIGMMEFSLFGISYTGADICGFFNDSEYHLCTRWTQLGAFYPFARNHNIQFTRRQDPVSWNQTFVEMTRNVLNIRYTLLPYFYTQLHEIHAHGGTVIRPLMHEFFDDRTTWDIFLQFLWGPAFMVTPVLEPYTTVVRGYVPNARWFDYHTGEDIGIRGQVQDLTLLMNAINLHVRGGHILPCQEPARTTFLSRQKYMKLIVAADDNHMAQGSLFWDDGDTIDTYERDLYLSVQFNLNKTTLTSTLLKTGYINKTEIRLGYVHVWGIGNTLINEVNLMYNEINYPLIFNQTQAQEILNIDLTAHEVTLDDPIEISWS

Target ORF information:

RefSeq Version	NM_001082266.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus sucrase-isomaltase (SI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082266.1

ORF Insert Sequence:

ATGGCCAAGA GGAAATTTTC TGGCTTAGAA ATCACTCTGA TTGTCCTCTT TGTGATAGTT 
TTTATAATAG CTATTGCTCT AATTGCTGTG TTAGCAACTA AGACACCAGC AGTTGAAGAG 
GTTAATCCTT CCAGTTCAAC TCCAACTACG ACTTCAACTA CAACTTCAAC TTCCGGTTCA 
GTAAGCTGTC CCAGTGAGCT AAATGAGGTT GTCAATGAGA GAATAAACTG CATTCCAGAA 
CAATCTCCAA CACAGGCGAT TTGTGCACAA CGAAACTGCT GTTGGAGACC ATGGAACAAC 
TCAGATATCC CATGGTGCTT CTTTGTAGAC AATCATGGCT ACAATGTTGA AGGAATGACA 
ACAACAAGTA CTGGACTTGA AGCCAGGTTA AACAGGAAAT CAACACCTAC ACTTTTTGGA 
AATGATATTA ACAATGTCCT CCTCACCACT GAAAGTCAGA CAGCCAATCG TTTACGGTTC 
AAGCTTACTG ATCCAAATAA TAAAAGATAT GAAGTTCCTC ATCAATTTGT GACAGAATTT 
GCTGGACCTG CAGCTACTGA GACATTATAT GATGTACAGG TTACAGAAAA TCCTTTTAGC 
ATCAAAGTTA TCAGGAAAAG TAACAACAGG ATTTTGTTTG ACAGCAGCAT TGGTCCCCTG 
GTGTACTCTG ACCAGTATTT ACAGATCTCA ACCAGACTTC CCAGTGAGTA CATGTATGGT 
TTTGGAGAAC ATGTTCACAA GAGATTTCGT CATGATTTAT ATTGGAAAAC ATGGCCGATC 
TTTACTCGGG ATCAACATAC TGATGATAAT AATAATAATT TATATGGCCA TCAGACATTC 
TTCATGTGTA TTGAAGACAC TACTGGAAAG TCATTTGGTG TTTTTTTAAT GAACAGCAAT 
GCAATGGAAA TTTTCATCCA GCCTACTCCA ATAGTAACCT ATAGAGTTAT CGGTGGCATC 
CTAGACTTTT ACATCTTTTT AGGAGATACA CCAGAACAGG TGGTCCAACA GTATCAAGAG 
CTCATTGGAC GACCAGCAAT GCCAGCATAC TGGAGTCTTG GATTCCAACT GAGTCGCTGG 
AATTACAATT CTCTAGATGT AGTCAAAGAG GTGGTAAGAA GAAACAGGGA AGCTCTCATA 
CCATTTGATA CACAAGTCAG TGATATTGAC TATATGGAAG ACAAGAAAGA CTTCACTTAT 
GATAGAGTGG CATATAATGG GCTCCCTGAC TTTGTTCAAG ATTTGCATGA CCATGGACAG 
AAATATGTTA TTATCTTGGA CCCTGCAATT TCTATAAATA GGCGTGCCAG TGGAGAAGCA 
TATGAATCCT ATGACAGAGG AAATGCACAA AATGTGTGGG TAAATGAGTC AGATGGAACT 
ACACCAATAG TTGGAGAGGT CTGGCCAGGA GATACAGTAT ATCCTGATTT CACCAGTCCA 
AACTGCATAG AATGGTGGGC AAATGAATGC AATATTTTCC ATCAAGAAGT GAATTATGAT 
GGACTTTGGA TTGACATGAA TGAAGTGTCC AGCTTTGTTC AAGGCTCAAA TAAGGGCTGT 
AATGACAACA CATTGAATTA TCCACCTTAC ATTCCTGATA TTGTTGATAA GCTCATGTAT 
TCCAAAACAC TTTGCATGGA TTCTGTGCAG TACTGGGGAA AACAGTATGA TGTTCACAGC 
CTATATGGAT ATAGCATGGC TATAGCCACT GAAAGAGCTG TAGAAAGAGT TTTCCCTAAC 
AAGAGAAGCT TCATCCTTAC TCGGTCAACT TTTGCTGGAA GTGGAAGACA TGCTGCACAT 
TGGCTAGGAG ATAACACGGC TACTTGGGAA CAAATGGAGT GGTCTATCAC TGGGATGCTA 
GAGTTCGGTT TGTTTGGAAT GCCTTTGGTT GGTGCAGATA TCTGTGGATT TCTGGCTGAA 
ACCACAGAAG AGCTTTGCAG AAGGTGGATG CAACTTGGGG CCTTTTATCC GTTTTCCAGA 
AATCATAATG CTGATGGATT TGAGCATCAG GATCCCGCAT TTTTTGGACA AGACTCACTT 
TTGGTTAAGT CATCTCGGCA CTACTTAAAT ATCCGCTATA CCTTATTGCC TTTCCTCTAT 
ACTCTATTTT ACAAAGCCCA TGCGTTTGGA GAAACAGTAG CAAGGCCAGT TCTTCATGAG 
TTTTATGAGG ACACAAACAG CTGGGTTGAG GACAGAGAGT TTTTATGGGG CCCCGCTTTA 
CTCATTACTC CTGTTTTGAC ACAGGGAGCA GAGACAGTGA GTGCATACAT TCCTGATGCT 
GTTTGGTATG ATTATGAAAC AGGTGCAAAA AGGCCATGGA GAAAGCAACG GGTGGAAATG 
AGTCTTCCAG CAGACAAAAT AGGACTGCAT CTTAGAGGAG GTTACATTAT CCCCATTCAA 
CAACCTGCTG TAACAACAAC AGCAAGTCGT ATGAATCCTC TAGGACTTAT AATTGCATTA 
AATGATGATA ACACAGCAGT AGGAGACTTT TTCTGGGATG ATGGAGAAAC TAAAGATACC 
GTACAAAATG ACAACTACAT ATTATATACA TTTGCAGTTT CTAACAACAA TTTAAATATC 
ACATGCACAC ATGAATTATA CTCAGAAGGA ACAACTTTAG CATTTCAGAC TATAAAAATT 
CTCGGGGTGA CGGAGACCGT TACACAGGTT ACAGTGGCAG AAAATAATCA GTCAATGAGT 
ACGCACTCTA ATTTCACATA TGATCCCTCC AACCAGGTTC TCTTAATTGA GAATCTCAAC 
TTTAACCTTG GAAGAAACTT CAGAGTTCAA TGGGATCAAA CTTTCTTAGA AAGCGAAAAG 
ATTACTTGTT ATCCAGATGC AGACATTGCA ACTCAGGAAA AGTGCACTCA ACGTGGATGT 
ATATGGGACA CGAATACTGT GAACCCTCGA GCACCTGAAT GTTACTTCCC TAAAACTGAC 
AACCCTTATT CAGTTAGCTC AACTCAGTAT TCACCGACGG GTATAACAGC TGACCTCCAG 
TTGAATCCTA CAAGAACCAG AATAACTTTA CCTTCTGAAC CCATTACAAA TCTTCGTGTG 
GAGGTGAAAT ATCACAAAAA TGATATGGTA CAGTTTAAGA TTTTTGATCC CCAAAACAAG 
AGATATGAAG TTCCGGTACC ACTAGACATT CCAGCCACTC CAACAAGCAC ACAGGAAAAC 
AGACTTTATG ATGTTGAAAT CAAGGAAAAC CCTTTTGGTA TCCAGATTCG ACGGAGAAGC 
ACTGGAAAAG TTATTTGGGA TTCTTGTCTA CCTGGTTTTG CTTTTAATGA TCAGTTCATT 
CAAATATCTA CTCGCCTGCC ATCGGAATAT ATATATGGTT TTGGAGAAGC AGAACACACA 
GCATTTAAGA GAGATCTGAA CTGGCATACT TGGGGGATGT TCACAAGAGA CCAACCCCCT 
GGTTACAAAC TTAATTCCTA TGGATTTCAT CCTTATTACA TGGCTCTGGA AGATGAAGGA 
AATGCCCATG GAGTTTTGTT ACTGAACAGC AATGCAATGG ATGTTACATT CATGCCAACT 
CCAGCTCTAA CTTATCGTGT AATTGGAGGA ATCTTGGATT TTTATATGTT CTTAGGCCCA 
ACTCCAGAAG TTGCAACCCA ACAATATCAT GAAGTAATTG GCCATCCAGT CATGCCACCG 
TATTGGTCTT TAGGATTCCA ATTATGTCGG TATGGATACA GAAATACTTC AGAGATTATT 
GAACTATATG AGGGGATGGT GGCTGCTGAC ATACCCTATG ATGTTCAATA CACAGATATT 
GATTACATGG AAAGGCAACT AGACTTTACA ATTGATGAAA ACTTTCGTGA ACTTCCTCAA 
TTTGTTGATA GAATAAGAGG AGAAGGAATG AGATACATTA TTATTCTGGA CCCAGCAATT 
TCAGGAAATG AAACACGGCC TTACCCTGCA TTTGACAGAG GGGAAGCTAA AGATGTCTTC 
GTCAAATGGC CTAATACCAG TGATATTTGT TGGGCAAAGG TTTGGCCAGA CTTGCCTAAT 
ATAACAATAG ATGAAAGTCT AACTGAAGAT GAAGCTGTTA ACGCTTCCCG AGCTCACGCA 
GCTTTTCCAG ATTTCTTCAG GAATTCCACA GCAGAGTGGT GGACAAGAGA AATTTTAGAT 
TTCTACAATA ACTATATGAA GTTTGATGGC TTGTGGATTG ATATGAATGA ACCATCAAGT 
TTTGTGAATG GAACAACTAC TAATGTATGC AGAAATACAG AGCTAAATTA TCCACCTTAT 
TTCCCAGAAC TTACAAAACG GACAGATGGA TTGCATTTCA GAACTATGTG CATGGAAACT 
GAGCATATTC TTAGTGATGG ATCATCAGTT TTACATTATG ATGTTCACAA TCTCTATGGA 
TGGTCACAAG CAAAGCCTAC TTATGATGCC TTGCAGAAGA CAACTGGCAA AAGAGGGATA 
GTTATTTCTC GTTCTACATA TCCTACTGCT GGGCGATGGG CAGGACACTG GCTTGGAGAC 
AACTATGCAC GGTGGGACAA TATGGACAAA TCGATCATTG GTATGATGGA ATTTAGTCTC 
TTTGGAATAT CATATACTGG AGCAGACATC TGTGGTTTCT TCAATGATTC AGAATATCAT 
CTCTGTACTC GCTGGACACA ACTTGGAGCA TTTTATCCAT TTGCAAGAAA TCACAACATT 
CAATTTACTA GAAGACAAGA CCCTGTTTCA TGGAATCAAA CTTTTGTTGA AATGACAAGG 
AATGTTCTGA ATATTAGATA TACTTTATTA CCTTATTTCT ATACACAATT GCATGAAATT 
CATGCTCATG GTGGCACTGT TATCCGACCC CTTATGCATG AGTTCTTTGA TGACAGAACA 
ACTTGGGACA TATTTCTACA GTTTCTGTGG GGTCCAGCAT TTATGGTTAC CCCTGTGTTG 
GAACCTTATA CTACTGTTGT ACGTGGCTAT GTCCCTAATG CTCGGTGGTT TGACTATCAT 
ACGGGAGAAG ATATTGGAAT CAGAGGACAA GTTCAAGATT TAACACTCCT TATGAATGCA 
ATAAATCTAC ATGTTCGTGG TGGTCACATC CTACCATGTC AGGAACCTGC TCGAACCACA 
TTTTTAAGTC GACAGAAGTA CATGAAGCTC ATTGTTGCTG CAGATGATAA TCACATGGCA 
CAAGGATCTC TCTTCTGGGA TGATGGGGAC ACTATAGATA CCTATGAGAG AGACCTGTAT 
TTATCAGTAC AATTTAATTT AAATAAGACA ACTTTGACAA GCACGCTATT GAAAACTGGT 
TACATAAATA AAACTGAAAT AAGACTTGGA TATGTCCATG TATGGGGCAT AGGAAACACT 
TTGATCAATG AAGTTAATTT AATGTATAAT GAAATAAATT ATCCACTTAT ATTTAACCAA 
ACCCAAGCCC AAGAGATATT AAATATTGAT CTGACGGCAC ATGAAGTTAC TCTAGATGAC 
CCAATAGAAA TCTCCTGGTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The sucrase-isomaltase complex: primary structure, membrane-orientation, and evolution of a stalked, intrinsic brush border protein.
Cell46(2)227-34(1986 Jul)
Hunziker W,Spiess M,Semenza G,Lodish HF

N-Terminal sequences of pig intestinal sucrase-isomaltase and pro-sucrase--isomaltase. Implications for the biosynthesis and membrane insertion of pro-sucrase--isomaltase.
FEBS letters148(2)321-5(1982 Nov)
Sjöström H,Norén O,Christiansen LA,Wacker H,Spiess M,Bigler-Meier B,Rickli EE,Semenza G

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