AGL cDNA ORF clone, Oryctolagus cuniculus(rabbit)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGL
Entrez Gene ID	100009066
Full Name	amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Gene Type	protein-coding
Organism	Oryctolagus cuniculus(rabbit)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008263968.2	XP_008262190.1	glycogen debranching enzyme isoform X1
XM_008263966.2	XP_008262188.1	glycogen debranching enzyme isoform X1
XM_008263969.2	XP_008262191.1	glycogen debranching enzyme isoform X1
XM_017345486.1	XP_017200975.1	glycogen debranching enzyme isoform X1
NM_001082247.1	NP_001075716.1	glycogen debranching enzyme

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Molecular cloning, sequencing, and analysis of the cDNA for rabbit muscle glycogen debranching enzyme.
Liu W, de Castro ML, Takrama J, Bilous PT, Vinayagamoorthy T, Madsen NB, Bleackley RC
Archives of biochemistry and biophysics306(1)232-9(1993 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AGL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOb30325	NM_001082247.1 Latest version!	Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOb13095	XM_017345486.1 Latest version!	Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOb13095	XM_008263966.2 Latest version!	Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOb13095	XM_008263969.2 Latest version!	Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OOb13095	XM_008263968.2 Latest version!	Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGL ( NM_001082247.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> NP_001075716.1 Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), mRNA.

Price & Availability↑

CloneID	OOb30325
Clone ID Related Accession (Same CDS sequence)	NM_001082247.1
Accession Version	NM_001082247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569168000000
Organism	Oryctolagus cuniculus(rabbit)
Product	glycogen debranching enzyme
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L10605.1. On Jun 22, 2014 this sequence version replaced XM_008263967.1. ##Evidence-Data-START## Transcript exon combination :: L10605.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1876818, SAMEA1876819 [ECO:0000350] ##Evidence-Data-END##

ATGGGTAACT CATTCGACTT TGGAGTTCTT TTAATTCTTC TGAAATATTT CAAGTCTTCT 
AGAAGCCAAA ATGGACACAG TAAACAGATT CGAATTTTAC TACTGAACGA AATGGAAAAG 
CTGGAAAAGA CCCTCTTCAG ACTTGAACAA GGATTTGAGC TACAATTTCG ATTAGGCCCA 
ACTTTACAGG GAAAACCAGT TACTGTGTTT ACAAATTATC CATTTCCTGG AGAAACATTT 
AATCGAGAAA AATTCCGGTC CCTGGAGTGG GAAAATCCAA CAGAAAGAGA AGATGATTCT 
GATAAATACT GCAAACTCAA TCTGCAGCAG TCTGGTTCAT TTCAGTATTA CTTCCTTCAA 
GGAAATGAGA AAAGTGGTGG AGGTTACATA GTTGTGGATC CTATTTTACG TGTTGGTGCT 
GATAATCACA TGTTACACTT GGACTGTGTC ACTCTCCAGA CATTTTTAGC CAAGTGTTTG 
GGACCCTTTG ATGAATGGGA AAGCAGACTT AGGGTTGCAA AAGAATCAGG TTACAACATG 
ATCCATTTTA CCCCACTGCA GACTCTTGGA CTGTCTAGGT CGTGCTACTC CCTTGCTGAC 
CAATTAGAAT TAAATCCCGA CTTCTCAAGG CCTCATAAAA AATACACGTG GAGTGATGTG 
GGACAGTTAG TGGAAAAACT GAAAAGGGAA TGGAATGTTC TTTGCATTAC AGATGTTGTC 
TACAATCATA CCGCTGCTAA TAGTAAATGG ATTCAGGAAC ATCCAGAATG TGCATATAAT 
CTTGTGAACT CACCACACTT AAAACCTGCC TGGGTCTTAG ACAGAGCACT GTGGCATTTC 
TCCTGTGATG TTGCGGAAGG AAAATACAAA AACAGAGGAG TACCTGCTTT GATTGAGAAT 
GATCATCATT TGAATTGCAT TCGAAAAGTA ATTTGGGAGG ATATTTTTCC AAAGCTTCAT 
CTCTGGGAAT TTTTCCAAGT AGACGTTTAC AAAGCAGTTG AGAAATTTAG AGGACTTCTA 
ACACAAGAAA CTTGGAGGGT AATCAAGTCA GATCCAAAAC AACATCTCAA GATTATCCAG 
GATCCTGAAT ACAGACGGTT TGGCTGTACT GTAGATATGA ACATTGCACT AGCAACTTTC 
ATACCACACG ACAATGGGCC AGCTGCAATT GAAGAATGCT GTAACTGGTT CCGGAAGAGA 
ATTGAGGAAT TAAATTCAGA GAAGCATCAA CTCATGAACT ATCATCAGGA ACAGGCAGTT 
AACTGTCTTT TGGGAAATGT GTTTTATGAA CGACTGGCTG GCCATGGTCC TAAATTAGGA 
CCTGTCACTA GAAAATATCC TTTAGTTACC AGGTACTTTA CTTTTCCATT TGAGGAAATG 
CCTGTATCTA CAGAAGAAAC TATGATTCAC CTCCCAAACA AAGCTTGTTT CTTCATGGCA 
CATAATGGTT GGGTAATGGG AGATGATCCT CTTCGAAACT TTGCTGAACC AGGTTCAGAT 
GTTTATTTAA GAAGAGAACT TATTTGCTGG GGAGACAGTG TTAAATTACG CTATGGAACT 
AAACCAGAGG ACTGTCCTTA TCTCTGGGCA CACATGAGAA AATACACTGA AATAATTGCA 
ACTTATTTCC AAGGAGTACG TCTTGATAAT TGCCATTCAA CACCTCTTCA TGTAGCCGAG 
TACATGTTGG ATGCTGCTAG GAAATTGCAA CCCAATTTAT ATGTAGTAGC TGAACTGTTC 
ACAGGAAGTG AAGATCTGGA CAACATCTTT GTTACCAGAC TGGGCATTAG TTCCTTAATA 
AGAGAGGCAA TGAGTGCATA TAATAGTCAT GAAGAAGGCA GATTAGTTTA CCGATATGGA 
GGAGAACCTG TTGGATCCTT TGTTCAGCCC TGTTTGAGGC CTTTAATGCC GGCCATCGCA 
CACGCTCTGT TTATGGATAT TACCCATGAT AATGAGTGCC CTATTGTGCA TAGATCAGTG 
TACGATGCTC TTCCAAGTAC CACAATTGTT TCTATGGCTT GTTGTGCTAG TGGAAGTACA 
AGGGGCTATG ATGAATTAGT GCCACATCAG ATTTCAGTGG TTTCTGAAGA ACGTTTTTAC 
ACTAAATGGA ACCCTGAAGC CTTGCCATCA AATGCTGGTG AAGTTAATTT CCAAAGTGGA 
ATTATTGCAG CCAGGTGTGC TATCAATAAA CTTCATCAAG AGCTTGGAGC CAAAGGTTTT 
ATTCAGGTGT ATGTGGATCA AGTTGATGAA GATATAGTGG CAGTAACAAG ACACTCACCA 
AGTATCCATC AGTCTTTTGT GGCCGTATCT AGAACTGCTT TCAGGAATCC CAAGACTTCA 
TTTTACAGCA AGGATGTGCC TCAAATGTGC ATCCCTGGTA AAATTGAAGA AGTAGTTCTT 
GAAGCTCGAA CTATTGAGAG AAATATAAGT CCTTACAGGA AGGATGAGAA TTCGATAAAT 
GGAATGCCAA ATATCACAGT AGAAATTAGA GAACATATTC AGCTTAATGA AAGTAGAATT 
GTTAAACAAG CTGGAGTTAC TACAAAAGGT CCCAATGAAT ATATTCAAGA AATAGAATTT 
GAAAACTTGT CTCCAGGAAG TGTTATTATA TTCAGAGTTA GTCTTGATCC ACATGCACAA 
GTTGCTGTTG GAATTCTTCG AAATCATCTG ACACAGTTCA GTGCTCACTT TAAAGCAGGA 
AGCCTTGCTG TTGACAACTC AGATCCAATA TTAAAAATTC CTTTTGCTTC TATTGCCTCC 
AAATTAACTT TAGCTGAGAT AAATCAGATA CTTTACCGGT GTGAATCAGA AGAACAAGAA 
GATGGTGGAG GATGCTATGA CATACCAAAC TGGTCTTCCC TTAAATATGC AGGTCTGCAA 
GGGTTAATGT CCGTATTGGC AGAAATACGA CCAAAGAATG ACTTGGGACA CCCTTTCTGT 
GATAATTTGC GGTCTGGAGA TTGGATGATT GACTATGTCA GTGGCCGGCT TATTTCACGA 
TCAGGAACTA TTGCTGAAGT TGGTAAATGG TTGCAGGCTA TGTTCTTCTA CCTGAAGCAG 
ATCCCACGTT ATCTTATACC ATGTTACTTT GATGCTATAT TAATTGGTGC ATACACCACT 
CTTCTGGATA TAGCATGGAA GCAAATGTCA AGTTTTGTTC AGACTGGTTC AACCTTTGTC 
AAACACCTCT CTCTGGGTTC AGTCCAAATG TGTGGAGTAG GAAAATTCCC TTCTCTGCCA 
CTTCTTTCAC CTTCACTTAC GGATGTCCCA TATAGGCTAA ATGAGATCAC AAAAGAAAAG 
GAGCAATGTT GTGTTTCTCT GGCTGCAGGC TTACCTCATT TTTCTTCTGG TATTTTTCGC 
TGTTGGGGAA GAGACACTTT TATTGCACTT AGAGGTTTGC TGCTGATTAC TGGACGCTAC 
TTAGAAGCCA GGAATATTAT TTTAGCATTT GCTGGTACCC TGAGGCATGG TCTCATTCCT 
AATCTCCTGG GTGAAGGAAC TTATGCCAGA TACAATTGCC GTGATGCTGT GTGGTGGTGG 
CTACAGTGTA TTCAGGATTA CTGTAAAATG GTTCCAAATG GTCTAGACAT CCTCAAGTGC 
CCAGTCTCCA GAATGTATCC TACAGATGAT TCTGCCCCTT TGCCTGCTGG CACACTGGAT 
CAGCCATTGT TTGATGTAAT ACAGGAGGCT ATGCAAAGAC ACATGCAGGG CATCCAGTTT 
CGGGAAAGGA ATGCTGGTCC ACAAATAGAC CGAAACATGA AGGATGAAGG TTTTACTGTA 
ATTGCTGGGG TTAATGAAGA AACAGGATTT GTTTATGGAG GAAATCGCTT CAATTGTGGT 
ACGTGGATGG ATAAAATGGG AGAAAGTGAC AGAGCGAGAA ACAGAGGGAT CCCAGCCACT 
CCCAGAGATG GGTCTGCTGT GGAAATTGTG GGCCTATGTA AATCTACAGT TCGTTGGTTG 
CTGGAGTTAT CCAAAAAAAA TATTTTCCCT TATCATGAAG TCAGAGTAAA AAGACATGGA 
AAGGTGGTAA CGGTCTCATA TGAGGAGTGG AACAGGAAAA TACAAGACAA CTTTGAAAAG 
CGGTTTCATG TTTCAGAAGA TCCTTCAGCA TCCAATGAAG AACATCCAAA TCTGGTTCAC 
AAGCGTGGCA TCTACAAAGA TAGTTACGGA GCTTCAAGTC CTTGGTGTGA CTACCAGCTC 
AGGCCCAACT TTACAATAGC AATGGTTGTG GCCCCAGAAC TCTTTACTGC AGAAAAAGCA 
TGGAAAGCTT TGGAGATTGC AGAAAAAAAA TTGCTTGGTC CCCTGGGCAT GAAAACTTTA 
GATCCAGATG ATATGGTTTA CTGTGGAATT TACGACAATG CCTTAGACAA TGACAACTAT 
AATCTTGCTA AAGGTTTCAA TTATCACCAA GGACCTGAGT GGCTGTGGCC TGTTGGTTAT 
TTTCTTCGTG CAAAACTATA TTTTTCCAAA TTGATGGATC GGGAGACTAA TGCAAGGACT 
ATATTTTTGG TTAAAAATGT TCTTTCTCGG CATTATGTTC ATCTTGAGAG GTCCCCTTGG 
AAAGGACTCC CAGAACTGAC CAACGAGAAT GGCCAGTACT GTCCTTTCAG CTGTGAAACA 
CAAGCCTGGT CAATTGCTAC TATTCTTGAA ACACTTTATG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075716.1
CDS	236..4903
Translation	MGNSFDFGVLLILLKYFKSSRSQNGHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKPVTVFTNYPFPGETFNREKFRSLEWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHMLHLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPHKKYTWSDVGQLVEKLKREWNVLCITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWHFSCDVAEGKYKNRGVPALIENDHHLNCIRKVIWEDIFPKLHLWEFFQVDVYKAVEKFRGLLTQETWRVIKSDPKQHLKIIQDPEYRRFGCTVDMNIALATFIPHDNGPAAIEECCNWFRKRIEELNSEKHQLMNYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKYPLVTRYFTFPFEEMPVSTEETMIHLPNKACFFMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGTKPEDCPYLWAHMRKYTEIIATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSVYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNAGEVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSFVAVSRTAFRNPKTSFYSKDVPQMCIPGKIEEVVLEARTIERNISPYRKDENSINGMPNITVEIREHIQLNESRIVKQAGVTTKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSAHFKAGSLAVDNSDPILKIPFASIASKLTLAEINQILYRCESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSGRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQTGSTFVKHLSLGSVQMCGVGKFPSLPLLSPSLTDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFAGTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFDVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFTVIAGVNEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLCKSTVRWLLELSKKNIFPYHEVRVKRHGKVVTVSYEEWNRKIQDNFEKRFHVSEDPSASNEEHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSKLMDRETNARTIFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	NM_001082247.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082247.1

ORF Insert Sequence:

ATGGGTAACT CATTCGACTT TGGAGTTCTT TTAATTCTTC TGAAATATTT CAAGTCTTCT 
AGAAGCCAAA ATGGACACAG TAAACAGATT CGAATTTTAC TACTGAACGA AATGGAAAAG 
CTGGAAAAGA CCCTCTTCAG ACTTGAACAA GGATTTGAGC TACAATTTCG ATTAGGCCCA 
ACTTTACAGG GAAAACCAGT TACTGTGTTT ACAAATTATC CATTTCCTGG AGAAACATTT 
AATCGAGAAA AATTCCGGTC CCTGGAGTGG GAAAATCCAA CAGAAAGAGA AGATGATTCT 
GATAAATACT GCAAACTCAA TCTGCAGCAG TCTGGTTCAT TTCAGTATTA CTTCCTTCAA 
GGAAATGAGA AAAGTGGTGG AGGTTACATA GTTGTGGATC CTATTTTACG TGTTGGTGCT 
GATAATCACA TGTTACACTT GGACTGTGTC ACTCTCCAGA CATTTTTAGC CAAGTGTTTG 
GGACCCTTTG ATGAATGGGA AAGCAGACTT AGGGTTGCAA AAGAATCAGG TTACAACATG 
ATCCATTTTA CCCCACTGCA GACTCTTGGA CTGTCTAGGT CGTGCTACTC CCTTGCTGAC 
CAATTAGAAT TAAATCCCGA CTTCTCAAGG CCTCATAAAA AATACACGTG GAGTGATGTG 
GGACAGTTAG TGGAAAAACT GAAAAGGGAA TGGAATGTTC TTTGCATTAC AGATGTTGTC 
TACAATCATA CCGCTGCTAA TAGTAAATGG ATTCAGGAAC ATCCAGAATG TGCATATAAT 
CTTGTGAACT CACCACACTT AAAACCTGCC TGGGTCTTAG ACAGAGCACT GTGGCATTTC 
TCCTGTGATG TTGCGGAAGG AAAATACAAA AACAGAGGAG TACCTGCTTT GATTGAGAAT 
GATCATCATT TGAATTGCAT TCGAAAAGTA ATTTGGGAGG ATATTTTTCC AAAGCTTCAT 
CTCTGGGAAT TTTTCCAAGT AGACGTTTAC AAAGCAGTTG AGAAATTTAG AGGACTTCTA 
ACACAAGAAA CTTGGAGGGT AATCAAGTCA GATCCAAAAC AACATCTCAA GATTATCCAG 
GATCCTGAAT ACAGACGGTT TGGCTGTACT GTAGATATGA ACATTGCACT AGCAACTTTC 
ATACCACACG ACAATGGGCC AGCTGCAATT GAAGAATGCT GTAACTGGTT CCGGAAGAGA 
ATTGAGGAAT TAAATTCAGA GAAGCATCAA CTCATGAACT ATCATCAGGA ACAGGCAGTT 
AACTGTCTTT TGGGAAATGT GTTTTATGAA CGACTGGCTG GCCATGGTCC TAAATTAGGA 
CCTGTCACTA GAAAATATCC TTTAGTTACC AGGTACTTTA CTTTTCCATT TGAGGAAATG 
CCTGTATCTA CAGAAGAAAC TATGATTCAC CTCCCAAACA AAGCTTGTTT CTTCATGGCA 
CATAATGGTT GGGTAATGGG AGATGATCCT CTTCGAAACT TTGCTGAACC AGGTTCAGAT 
GTTTATTTAA GAAGAGAACT TATTTGCTGG GGAGACAGTG TTAAATTACG CTATGGAACT 
AAACCAGAGG ACTGTCCTTA TCTCTGGGCA CACATGAGAA AATACACTGA AATAATTGCA 
ACTTATTTCC AAGGAGTACG TCTTGATAAT TGCCATTCAA CACCTCTTCA TGTAGCCGAG 
TACATGTTGG ATGCTGCTAG GAAATTGCAA CCCAATTTAT ATGTAGTAGC TGAACTGTTC 
ACAGGAAGTG AAGATCTGGA CAACATCTTT GTTACCAGAC TGGGCATTAG TTCCTTAATA 
AGAGAGGCAA TGAGTGCATA TAATAGTCAT GAAGAAGGCA GATTAGTTTA CCGATATGGA 
GGAGAACCTG TTGGATCCTT TGTTCAGCCC TGTTTGAGGC CTTTAATGCC GGCCATCGCA 
CACGCTCTGT TTATGGATAT TACCCATGAT AATGAGTGCC CTATTGTGCA TAGATCAGTG 
TACGATGCTC TTCCAAGTAC CACAATTGTT TCTATGGCTT GTTGTGCTAG TGGAAGTACA 
AGGGGCTATG ATGAATTAGT GCCACATCAG ATTTCAGTGG TTTCTGAAGA ACGTTTTTAC 
ACTAAATGGA ACCCTGAAGC CTTGCCATCA AATGCTGGTG AAGTTAATTT CCAAAGTGGA 
ATTATTGCAG CCAGGTGTGC TATCAATAAA CTTCATCAAG AGCTTGGAGC CAAAGGTTTT 
ATTCAGGTGT ATGTGGATCA AGTTGATGAA GATATAGTGG CAGTAACAAG ACACTCACCA 
AGTATCCATC AGTCTTTTGT GGCCGTATCT AGAACTGCTT TCAGGAATCC CAAGACTTCA 
TTTTACAGCA AGGATGTGCC TCAAATGTGC ATCCCTGGTA AAATTGAAGA AGTAGTTCTT 
GAAGCTCGAA CTATTGAGAG AAATATAAGT CCTTACAGGA AGGATGAGAA TTCGATAAAT 
GGAATGCCAA ATATCACAGT AGAAATTAGA GAACATATTC AGCTTAATGA AAGTAGAATT 
GTTAAACAAG CTGGAGTTAC TACAAAAGGT CCCAATGAAT ATATTCAAGA AATAGAATTT 
GAAAACTTGT CTCCAGGAAG TGTTATTATA TTCAGAGTTA GTCTTGATCC ACATGCACAA 
GTTGCTGTTG GAATTCTTCG AAATCATCTG ACACAGTTCA GTGCTCACTT TAAAGCAGGA 
AGCCTTGCTG TTGACAACTC AGATCCAATA TTAAAAATTC CTTTTGCTTC TATTGCCTCC 
AAATTAACTT TAGCTGAGAT AAATCAGATA CTTTACCGGT GTGAATCAGA AGAACAAGAA 
GATGGTGGAG GATGCTATGA CATACCAAAC TGGTCTTCCC TTAAATATGC AGGTCTGCAA 
GGGTTAATGT CCGTATTGGC AGAAATACGA CCAAAGAATG ACTTGGGACA CCCTTTCTGT 
GATAATTTGC GGTCTGGAGA TTGGATGATT GACTATGTCA GTGGCCGGCT TATTTCACGA 
TCAGGAACTA TTGCTGAAGT TGGTAAATGG TTGCAGGCTA TGTTCTTCTA CCTGAAGCAG 
ATCCCACGTT ATCTTATACC ATGTTACTTT GATGCTATAT TAATTGGTGC ATACACCACT 
CTTCTGGATA TAGCATGGAA GCAAATGTCA AGTTTTGTTC AGACTGGTTC AACCTTTGTC 
AAACACCTCT CTCTGGGTTC AGTCCAAATG TGTGGAGTAG GAAAATTCCC TTCTCTGCCA 
CTTCTTTCAC CTTCACTTAC GGATGTCCCA TATAGGCTAA ATGAGATCAC AAAAGAAAAG 
GAGCAATGTT GTGTTTCTCT GGCTGCAGGC TTACCTCATT TTTCTTCTGG TATTTTTCGC 
TGTTGGGGAA GAGACACTTT TATTGCACTT AGAGGTTTGC TGCTGATTAC TGGACGCTAC 
TTAGAAGCCA GGAATATTAT TTTAGCATTT GCTGGTACCC TGAGGCATGG TCTCATTCCT 
AATCTCCTGG GTGAAGGAAC TTATGCCAGA TACAATTGCC GTGATGCTGT GTGGTGGTGG 
CTACAGTGTA TTCAGGATTA CTGTAAAATG GTTCCAAATG GTCTAGACAT CCTCAAGTGC 
CCAGTCTCCA GAATGTATCC TACAGATGAT TCTGCCCCTT TGCCTGCTGG CACACTGGAT 
CAGCCATTGT TTGATGTAAT ACAGGAGGCT ATGCAAAGAC ACATGCAGGG CATCCAGTTT 
CGGGAAAGGA ATGCTGGTCC ACAAATAGAC CGAAACATGA AGGATGAAGG TTTTACTGTA 
ATTGCTGGGG TTAATGAAGA AACAGGATTT GTTTATGGAG GAAATCGCTT CAATTGTGGT 
ACGTGGATGG ATAAAATGGG AGAAAGTGAC AGAGCGAGAA ACAGAGGGAT CCCAGCCACT 
CCCAGAGATG GGTCTGCTGT GGAAATTGTG GGCCTATGTA AATCTACAGT TCGTTGGTTG 
CTGGAGTTAT CCAAAAAAAA TATTTTCCCT TATCATGAAG TCAGAGTAAA AAGACATGGA 
AAGGTGGTAA CGGTCTCATA TGAGGAGTGG AACAGGAAAA TACAAGACAA CTTTGAAAAG 
CGGTTTCATG TTTCAGAAGA TCCTTCAGCA TCCAATGAAG AACATCCAAA TCTGGTTCAC 
AAGCGTGGCA TCTACAAAGA TAGTTACGGA GCTTCAAGTC CTTGGTGTGA CTACCAGCTC 
AGGCCCAACT TTACAATAGC AATGGTTGTG GCCCCAGAAC TCTTTACTGC AGAAAAAGCA 
TGGAAAGCTT TGGAGATTGC AGAAAAAAAA TTGCTTGGTC CCCTGGGCAT GAAAACTTTA 
GATCCAGATG ATATGGTTTA CTGTGGAATT TACGACAATG CCTTAGACAA TGACAACTAT 
AATCTTGCTA AAGGTTTCAA TTATCACCAA GGACCTGAGT GGCTGTGGCC TGTTGGTTAT 
TTTCTTCGTG CAAAACTATA TTTTTCCAAA TTGATGGATC GGGAGACTAA TGCAAGGACT 
ATATTTTTGG TTAAAAATGT TCTTTCTCGG CATTATGTTC ATCTTGAGAG GTCCCCTTGG 
AAAGGACTCC CAGAACTGAC CAACGAGAAT GGCCAGTACT GTCCTTTCAG CTGTGAAACA 
CAAGCCTGGT CAATTGCTAC TATTCTTGAA ACACTTTATG ATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_008263968.2 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_008262190.1 Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOb13095
Clone ID Related Accession (Same CDS sequence)	XM_008263968.2 , XM_008263966.2 , XM_008263969.2 , XM_017345486.1
Accession Version	XM_008263968.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2016 this sequence version replaced XM_008263968.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAG CTACAATTTC GATTAGGCCC AACTTTACAG 
GGAAAACCAG TTACTGTATT TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGAGAA 
AAATTCCGGT CCCTGGAGTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGCAAACTCA ATCTGCAACA GTCTGGTTCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATGTTACACT TGGACTGTGT CACTCTCCAG ACATTTTTAG CCAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GTTACAACAT GATCCATTTT 
ACCCCACTGC AGACTCTTGG ACTGTCTAGG TCGTGCTACT CCCTTGCTGA CCAATTAGAA 
TTAAATCCCG ACTTCTCAAG GCCTCATAAA AAATACACGT GGAGTGATGT GGGACAGTTA 
GTGGAAAAAC TGAAAAGGGA ATGGAATGTT CTTTGCATTA CAGATGTTGT CTACAATCAT 
ACCGCTGCTA ATAGTAAATG GATTCAGGAA CATCCAGAAT GTGCATATAA TCTTGTGAAC 
TCACCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT CTCCTGTGAT 
GTTGCGGAAG GAAAATACAA AAACAGAGGA GTACCTGCTT TGATTGAGAA TGATCATCAT 
TTGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGCTTCA TCTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGAAATTTA GAGGACTTCT AACACAAGAA 
ACTTGGAGGG TAATCAAGTC AGATCCAAAA CAACATCTCA AGATTATCCA GGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAGCAACTTT CATACCACAC 
GACAATGGGC CAGCTGCAAT TGAAGAATGC TGTAACTGGT TCCGGAAGAG AATTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTCATGAAC TATCATCAGG AACAGGCAGT TAACTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CTAAATTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGGTACTTT ACTTTTCCAT TTGAGGAAAT GCCTGTATCT 
ACAGAAGAAA CTATGATTCA CCTCCCAAAC AAAGCTTGTT TCTTCATGGC ACATAATGGT 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CAGGTTCAGA TGTTTATTTA 
AGAAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGAAC TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAGA AAATACACTG AAATAATTGC AACTTATTTC 
CAAGGAGTAC GTCTTGATAA TTGCCATTCA ACACCTCTTC ATGTAGCCGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAACATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAAGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CGGCCATCGC ACACGCTCTG 
TTTATGGATA TTACCCATGA TAATGAGTGC CCTATTGTGC ATAGATCAGT GTACGATGCT 
CTTCCAAGTA CCACAATTGT TTCTATGGCT TGTTGTGCTA GTGGAAGTAC AAGGGGCTAT 
GATGAATTAG TGCCACATCA GATTTCAGTG GTTTCTGAAG AACGTTTTTA CACTAAATGG 
AACCCTGAAG CCTTGCCATC AAATGCTGGT GAAGTTAATT TCCAAAGTGG AATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAA GAGCTTGGAG CCAAAGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGATATAGTG GCAGTAACAA GACACTCACC AAGTATCCAT 
CAGTCTGTTG TGGCCGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGATGTGC CTCAAATGTG CATCCCTGGT AAAATTGAAG AAGTAGTTCT TGAAGCTCGA 
ACTATTGAGA GAAATATAAG TCCTTACAGG AAGGATGAGA ATTCGATAAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAGAAT TGTTAAACAA 
GCTGGAGTTA CTACAAAAGG TCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTGCTCACT TTAAAGCAGG AAGCCTTGCT 
GTTGACAACT CAGATCCAAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAAATTAACT 
TTAGCTGAGA TAAATCAGAT ACTTTACCGG TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGATGCTATG ACATACCAAA CTGGTCTTCC CTTAAATATG CAGGTCTGCA AGGGTTAATG 
TCCGTATTGG CAGAAATACG ACCAAAGAAT GACTTGGGAC ACCCTTTCTG TGATAATTTG 
CGGTCTGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TATCTTATAC CATGTTACTT TGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAAATGTC AAGTTTTGTT CAGACTGGTT CAACCTTTGT CAAACACCTC 
TCTCTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAAATTCC CTTCTCTGCC ACTTCTTTCA 
CCTTCACTTA CGGATGTCCC ATATAGGCTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTTCG CTGTTGGGGA 
AGAGACACTT TTATTGCACT TAGAGGTTTG CTGCTGATTA CTGGACGCTA CTTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCTGGTACC CTGAGGCATG GTCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTTATGCCAG ATACAATTGC CGTGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TCCTCAAGTG CCCAGTCTCC 
AGAATGTATC CTACAGATGA TTCTGCCCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGATGTAA TACAGGAGGC TATGCAAAGA CACATGCAGG GCATCCAGTT TCGGGAAAGG 
AATGCTGGTC CACAAATAGA CCGAAACATG AAGGATGAAG GTTTTACTGT AATTGCTGGG 
GTTAATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGTGG TACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCGAGA AACAGAGGGA TCCCAGCCAC TCCCAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTATGT AAATCTACAG TTCGTTGGTT GCTGGAGTTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCAGAGTAA AAAGACATGG AAAGGTGGTA 
ACGGTCTCAT ATGAGGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCGGTTTCAT 
GTTTCAGAAG ATCCTTCAGC ATCCAATGAA GAACATCCAA ATCTGGTTCA CAAGCGTGGC 
ATCTACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCCAAC 
TTTACAATAG CAATGGTTGT GGCCCCAGAA CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTGGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTACGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTGTTGGTTA TTTTCTTCGT 
GCAAAACTAT ATTTTTCCAA ATTGATGGAT CGGGAGACTA ATGCAAGGAC TATATTTTTG 
GTTAAAAATG TTCTTTCTCG GCATTATGTT CATCTTGAGA GGTCCCCTTG GAAAGGACTC 
CCAGAACTGA CCAACGAGAA TGGCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA AACACTTTAT GATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008262190.1
CDS	242..4840
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKPVTVFTNYPFPGETFNREKFRSLEWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHMLHLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPHKKYTWSDVGQLVEKLKREWNVLCITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWHFSCDVAEGKYKNRGVPALIENDHHLNCIRKVIWEDIFPKLHLWEFFQVDVYKAVEKFRGLLTQETWRVIKSDPKQHLKIIQDPEYRRFGCTVDMNIALATFIPHDNGPAAIEECCNWFRKRIEELNSEKHQLMNYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKYPLVTRYFTFPFEEMPVSTEETMIHLPNKACFFMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGTKPEDCPYLWAHMRKYTEIIATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSVYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNAGEVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKDVPQMCIPGKIEEVVLEARTIERNISPYRKDENSINGMPNITVEIREHIQLNESRIVKQAGVTTKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSAHFKAGSLAVDNSDPILKIPFASIASKLTLAEINQILYRCESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSGRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQTGSTFVKHLSLGSVQMCGVGKFPSLPLLSPSLTDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFAGTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFDVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFTVIAGVNEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLCKSTVRWLLELSKKNIFPYHEVRVKRHGKVVTVSYEEWNRKIQDNFEKRFHVSEDPSASNEEHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSKLMDRETNARTIFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_008263968.2
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008263968.2

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAG CTACAATTTC GATTAGGCCC AACTTTACAG 
GGAAAACCAG TTACTGTATT TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGAGAA 
AAATTCCGGT CCCTGGAGTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGCAAACTCA ATCTGCAACA GTCTGGTTCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATGTTACACT TGGACTGTGT CACTCTCCAG ACATTTTTAG CCAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GTTACAACAT GATCCATTTT 
ACCCCACTGC AGACTCTTGG ACTGTCTAGG TCGTGCTACT CCCTTGCTGA CCAATTAGAA 
TTAAATCCCG ACTTCTCAAG GCCTCATAAA AAATACACGT GGAGTGATGT GGGACAGTTA 
GTGGAAAAAC TGAAAAGGGA ATGGAATGTT CTTTGCATTA CAGATGTTGT CTACAATCAT 
ACCGCTGCTA ATAGTAAATG GATTCAGGAA CATCCAGAAT GTGCATATAA TCTTGTGAAC 
TCACCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT CTCCTGTGAT 
GTTGCGGAAG GAAAATACAA AAACAGAGGA GTACCTGCTT TGATTGAGAA TGATCATCAT 
TTGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGCTTCA TCTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGAAATTTA GAGGACTTCT AACACAAGAA 
ACTTGGAGGG TAATCAAGTC AGATCCAAAA CAACATCTCA AGATTATCCA GGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAGCAACTTT CATACCACAC 
GACAATGGGC CAGCTGCAAT TGAAGAATGC TGTAACTGGT TCCGGAAGAG AATTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTCATGAAC TATCATCAGG AACAGGCAGT TAACTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CTAAATTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGGTACTTT ACTTTTCCAT TTGAGGAAAT GCCTGTATCT 
ACAGAAGAAA CTATGATTCA CCTCCCAAAC AAAGCTTGTT TCTTCATGGC ACATAATGGT 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CAGGTTCAGA TGTTTATTTA 
AGAAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGAAC TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAGA AAATACACTG AAATAATTGC AACTTATTTC 
CAAGGAGTAC GTCTTGATAA TTGCCATTCA ACACCTCTTC ATGTAGCCGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAACATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAAGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CGGCCATCGC ACACGCTCTG 
TTTATGGATA TTACCCATGA TAATGAGTGC CCTATTGTGC ATAGATCAGT GTACGATGCT 
CTTCCAAGTA CCACAATTGT TTCTATGGCT TGTTGTGCTA GTGGAAGTAC AAGGGGCTAT 
GATGAATTAG TGCCACATCA GATTTCAGTG GTTTCTGAAG AACGTTTTTA CACTAAATGG 
AACCCTGAAG CCTTGCCATC AAATGCTGGT GAAGTTAATT TCCAAAGTGG AATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAA GAGCTTGGAG CCAAAGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGATATAGTG GCAGTAACAA GACACTCACC AAGTATCCAT 
CAGTCTGTTG TGGCCGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGATGTGC CTCAAATGTG CATCCCTGGT AAAATTGAAG AAGTAGTTCT TGAAGCTCGA 
ACTATTGAGA GAAATATAAG TCCTTACAGG AAGGATGAGA ATTCGATAAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAGAAT TGTTAAACAA 
GCTGGAGTTA CTACAAAAGG TCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTGCTCACT TTAAAGCAGG AAGCCTTGCT 
GTTGACAACT CAGATCCAAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAAATTAACT 
TTAGCTGAGA TAAATCAGAT ACTTTACCGG TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGATGCTATG ACATACCAAA CTGGTCTTCC CTTAAATATG CAGGTCTGCA AGGGTTAATG 
TCCGTATTGG CAGAAATACG ACCAAAGAAT GACTTGGGAC ACCCTTTCTG TGATAATTTG 
CGGTCTGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TATCTTATAC CATGTTACTT TGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAAATGTC AAGTTTTGTT CAGACTGGTT CAACCTTTGT CAAACACCTC 
TCTCTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAAATTCC CTTCTCTGCC ACTTCTTTCA 
CCTTCACTTA CGGATGTCCC ATATAGGCTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTTCG CTGTTGGGGA 
AGAGACACTT TTATTGCACT TAGAGGTTTG CTGCTGATTA CTGGACGCTA CTTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCTGGTACC CTGAGGCATG GTCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTTATGCCAG ATACAATTGC CGTGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TCCTCAAGTG CCCAGTCTCC 
AGAATGTATC CTACAGATGA TTCTGCCCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGATGTAA TACAGGAGGC TATGCAAAGA CACATGCAGG GCATCCAGTT TCGGGAAAGG 
AATGCTGGTC CACAAATAGA CCGAAACATG AAGGATGAAG GTTTTACTGT AATTGCTGGG 
GTTAATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGTGG TACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCGAGA AACAGAGGGA TCCCAGCCAC TCCCAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTATGT AAATCTACAG TTCGTTGGTT GCTGGAGTTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCAGAGTAA AAAGACATGG AAAGGTGGTA 
ACGGTCTCAT ATGAGGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCGGTTTCAT 
GTTTCAGAAG ATCCTTCAGC ATCCAATGAA GAACATCCAA ATCTGGTTCA CAAGCGTGGC 
ATCTACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCCAAC 
TTTACAATAG CAATGGTTGT GGCCCCAGAA CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTGGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTACGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTGTTGGTTA TTTTCTTCGT 
GCAAAACTAT ATTTTTCCAA ATTGATGGAT CGGGAGACTA ATGCAAGGAC TATATTTTTG 
GTTAAAAATG TTCTTTCTCG GCATTATGTT CATCTTGAGA GGTCCCCTTG GAAAGGACTC 
CCAGAACTGA CCAACGAGAA TGGCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA AACACTTTAT GATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_008263966.2 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_008262188.1 Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

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CloneID	OOb13095
Clone ID Related Accession (Same CDS sequence)	XM_008263968.2 , XM_008263966.2 , XM_008263969.2 , XM_017345486.1
Accession Version	XM_008263966.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2016 this sequence version replaced XM_008263966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAG CTACAATTTC GATTAGGCCC AACTTTACAG 
GGAAAACCAG TTACTGTATT TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGAGAA 
AAATTCCGGT CCCTGGAGTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGCAAACTCA ATCTGCAACA GTCTGGTTCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATGTTACACT TGGACTGTGT CACTCTCCAG ACATTTTTAG CCAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GTTACAACAT GATCCATTTT 
ACCCCACTGC AGACTCTTGG ACTGTCTAGG TCGTGCTACT CCCTTGCTGA CCAATTAGAA 
TTAAATCCCG ACTTCTCAAG GCCTCATAAA AAATACACGT GGAGTGATGT GGGACAGTTA 
GTGGAAAAAC TGAAAAGGGA ATGGAATGTT CTTTGCATTA CAGATGTTGT CTACAATCAT 
ACCGCTGCTA ATAGTAAATG GATTCAGGAA CATCCAGAAT GTGCATATAA TCTTGTGAAC 
TCACCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT CTCCTGTGAT 
GTTGCGGAAG GAAAATACAA AAACAGAGGA GTACCTGCTT TGATTGAGAA TGATCATCAT 
TTGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGCTTCA TCTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGAAATTTA GAGGACTTCT AACACAAGAA 
ACTTGGAGGG TAATCAAGTC AGATCCAAAA CAACATCTCA AGATTATCCA GGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAGCAACTTT CATACCACAC 
GACAATGGGC CAGCTGCAAT TGAAGAATGC TGTAACTGGT TCCGGAAGAG AATTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTCATGAAC TATCATCAGG AACAGGCAGT TAACTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CTAAATTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGGTACTTT ACTTTTCCAT TTGAGGAAAT GCCTGTATCT 
ACAGAAGAAA CTATGATTCA CCTCCCAAAC AAAGCTTGTT TCTTCATGGC ACATAATGGT 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CAGGTTCAGA TGTTTATTTA 
AGAAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGAAC TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAGA AAATACACTG AAATAATTGC AACTTATTTC 
CAAGGAGTAC GTCTTGATAA TTGCCATTCA ACACCTCTTC ATGTAGCCGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAACATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAAGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CGGCCATCGC ACACGCTCTG 
TTTATGGATA TTACCCATGA TAATGAGTGC CCTATTGTGC ATAGATCAGT GTACGATGCT 
CTTCCAAGTA CCACAATTGT TTCTATGGCT TGTTGTGCTA GTGGAAGTAC AAGGGGCTAT 
GATGAATTAG TGCCACATCA GATTTCAGTG GTTTCTGAAG AACGTTTTTA CACTAAATGG 
AACCCTGAAG CCTTGCCATC AAATGCTGGT GAAGTTAATT TCCAAAGTGG AATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAA GAGCTTGGAG CCAAAGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGATATAGTG GCAGTAACAA GACACTCACC AAGTATCCAT 
CAGTCTGTTG TGGCCGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGATGTGC CTCAAATGTG CATCCCTGGT AAAATTGAAG AAGTAGTTCT TGAAGCTCGA 
ACTATTGAGA GAAATATAAG TCCTTACAGG AAGGATGAGA ATTCGATAAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAGAAT TGTTAAACAA 
GCTGGAGTTA CTACAAAAGG TCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTGCTCACT TTAAAGCAGG AAGCCTTGCT 
GTTGACAACT CAGATCCAAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAAATTAACT 
TTAGCTGAGA TAAATCAGAT ACTTTACCGG TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGATGCTATG ACATACCAAA CTGGTCTTCC CTTAAATATG CAGGTCTGCA AGGGTTAATG 
TCCGTATTGG CAGAAATACG ACCAAAGAAT GACTTGGGAC ACCCTTTCTG TGATAATTTG 
CGGTCTGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TATCTTATAC CATGTTACTT TGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAAATGTC AAGTTTTGTT CAGACTGGTT CAACCTTTGT CAAACACCTC 
TCTCTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAAATTCC CTTCTCTGCC ACTTCTTTCA 
CCTTCACTTA CGGATGTCCC ATATAGGCTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTTCG CTGTTGGGGA 
AGAGACACTT TTATTGCACT TAGAGGTTTG CTGCTGATTA CTGGACGCTA CTTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCTGGTACC CTGAGGCATG GTCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTTATGCCAG ATACAATTGC CGTGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TCCTCAAGTG CCCAGTCTCC 
AGAATGTATC CTACAGATGA TTCTGCCCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGATGTAA TACAGGAGGC TATGCAAAGA CACATGCAGG GCATCCAGTT TCGGGAAAGG 
AATGCTGGTC CACAAATAGA CCGAAACATG AAGGATGAAG GTTTTACTGT AATTGCTGGG 
GTTAATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGTGG TACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCGAGA AACAGAGGGA TCCCAGCCAC TCCCAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTATGT AAATCTACAG TTCGTTGGTT GCTGGAGTTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCAGAGTAA AAAGACATGG AAAGGTGGTA 
ACGGTCTCAT ATGAGGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCGGTTTCAT 
GTTTCAGAAG ATCCTTCAGC ATCCAATGAA GAACATCCAA ATCTGGTTCA CAAGCGTGGC 
ATCTACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCCAAC 
TTTACAATAG CAATGGTTGT GGCCCCAGAA CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTGGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTACGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTGTTGGTTA TTTTCTTCGT 
GCAAAACTAT ATTTTTCCAA ATTGATGGAT CGGGAGACTA ATGCAAGGAC TATATTTTTG 
GTTAAAAATG TTCTTTCTCG GCATTATGTT CATCTTGAGA GGTCCCCTTG GAAAGGACTC 
CCAGAACTGA CCAACGAGAA TGGCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA AACACTTTAT GATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008262188.1
CDS	275..4873
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKPVTVFTNYPFPGETFNREKFRSLEWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHMLHLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPHKKYTWSDVGQLVEKLKREWNVLCITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWHFSCDVAEGKYKNRGVPALIENDHHLNCIRKVIWEDIFPKLHLWEFFQVDVYKAVEKFRGLLTQETWRVIKSDPKQHLKIIQDPEYRRFGCTVDMNIALATFIPHDNGPAAIEECCNWFRKRIEELNSEKHQLMNYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKYPLVTRYFTFPFEEMPVSTEETMIHLPNKACFFMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGTKPEDCPYLWAHMRKYTEIIATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSVYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNAGEVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKDVPQMCIPGKIEEVVLEARTIERNISPYRKDENSINGMPNITVEIREHIQLNESRIVKQAGVTTKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSAHFKAGSLAVDNSDPILKIPFASIASKLTLAEINQILYRCESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSGRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQTGSTFVKHLSLGSVQMCGVGKFPSLPLLSPSLTDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFAGTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFDVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFTVIAGVNEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLCKSTVRWLLELSKKNIFPYHEVRVKRHGKVVTVSYEEWNRKIQDNFEKRFHVSEDPSASNEEHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSKLMDRETNARTIFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_008263966.2
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008263966.2

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAG CTACAATTTC GATTAGGCCC AACTTTACAG 
GGAAAACCAG TTACTGTATT TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGAGAA 
AAATTCCGGT CCCTGGAGTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGCAAACTCA ATCTGCAACA GTCTGGTTCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATGTTACACT TGGACTGTGT CACTCTCCAG ACATTTTTAG CCAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GTTACAACAT GATCCATTTT 
ACCCCACTGC AGACTCTTGG ACTGTCTAGG TCGTGCTACT CCCTTGCTGA CCAATTAGAA 
TTAAATCCCG ACTTCTCAAG GCCTCATAAA AAATACACGT GGAGTGATGT GGGACAGTTA 
GTGGAAAAAC TGAAAAGGGA ATGGAATGTT CTTTGCATTA CAGATGTTGT CTACAATCAT 
ACCGCTGCTA ATAGTAAATG GATTCAGGAA CATCCAGAAT GTGCATATAA TCTTGTGAAC 
TCACCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT CTCCTGTGAT 
GTTGCGGAAG GAAAATACAA AAACAGAGGA GTACCTGCTT TGATTGAGAA TGATCATCAT 
TTGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGCTTCA TCTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGAAATTTA GAGGACTTCT AACACAAGAA 
ACTTGGAGGG TAATCAAGTC AGATCCAAAA CAACATCTCA AGATTATCCA GGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAGCAACTTT CATACCACAC 
GACAATGGGC CAGCTGCAAT TGAAGAATGC TGTAACTGGT TCCGGAAGAG AATTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTCATGAAC TATCATCAGG AACAGGCAGT TAACTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CTAAATTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGGTACTTT ACTTTTCCAT TTGAGGAAAT GCCTGTATCT 
ACAGAAGAAA CTATGATTCA CCTCCCAAAC AAAGCTTGTT TCTTCATGGC ACATAATGGT 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CAGGTTCAGA TGTTTATTTA 
AGAAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGAAC TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAGA AAATACACTG AAATAATTGC AACTTATTTC 
CAAGGAGTAC GTCTTGATAA TTGCCATTCA ACACCTCTTC ATGTAGCCGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAACATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAAGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CGGCCATCGC ACACGCTCTG 
TTTATGGATA TTACCCATGA TAATGAGTGC CCTATTGTGC ATAGATCAGT GTACGATGCT 
CTTCCAAGTA CCACAATTGT TTCTATGGCT TGTTGTGCTA GTGGAAGTAC AAGGGGCTAT 
GATGAATTAG TGCCACATCA GATTTCAGTG GTTTCTGAAG AACGTTTTTA CACTAAATGG 
AACCCTGAAG CCTTGCCATC AAATGCTGGT GAAGTTAATT TCCAAAGTGG AATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAA GAGCTTGGAG CCAAAGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGATATAGTG GCAGTAACAA GACACTCACC AAGTATCCAT 
CAGTCTGTTG TGGCCGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGATGTGC CTCAAATGTG CATCCCTGGT AAAATTGAAG AAGTAGTTCT TGAAGCTCGA 
ACTATTGAGA GAAATATAAG TCCTTACAGG AAGGATGAGA ATTCGATAAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAGAAT TGTTAAACAA 
GCTGGAGTTA CTACAAAAGG TCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTGCTCACT TTAAAGCAGG AAGCCTTGCT 
GTTGACAACT CAGATCCAAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAAATTAACT 
TTAGCTGAGA TAAATCAGAT ACTTTACCGG TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGATGCTATG ACATACCAAA CTGGTCTTCC CTTAAATATG CAGGTCTGCA AGGGTTAATG 
TCCGTATTGG CAGAAATACG ACCAAAGAAT GACTTGGGAC ACCCTTTCTG TGATAATTTG 
CGGTCTGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TATCTTATAC CATGTTACTT TGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAAATGTC AAGTTTTGTT CAGACTGGTT CAACCTTTGT CAAACACCTC 
TCTCTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAAATTCC CTTCTCTGCC ACTTCTTTCA 
CCTTCACTTA CGGATGTCCC ATATAGGCTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTTCG CTGTTGGGGA 
AGAGACACTT TTATTGCACT TAGAGGTTTG CTGCTGATTA CTGGACGCTA CTTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCTGGTACC CTGAGGCATG GTCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTTATGCCAG ATACAATTGC CGTGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TCCTCAAGTG CCCAGTCTCC 
AGAATGTATC CTACAGATGA TTCTGCCCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGATGTAA TACAGGAGGC TATGCAAAGA CACATGCAGG GCATCCAGTT TCGGGAAAGG 
AATGCTGGTC CACAAATAGA CCGAAACATG AAGGATGAAG GTTTTACTGT AATTGCTGGG 
GTTAATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGTGG TACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCGAGA AACAGAGGGA TCCCAGCCAC TCCCAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTATGT AAATCTACAG TTCGTTGGTT GCTGGAGTTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCAGAGTAA AAAGACATGG AAAGGTGGTA 
ACGGTCTCAT ATGAGGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCGGTTTCAT 
GTTTCAGAAG ATCCTTCAGC ATCCAATGAA GAACATCCAA ATCTGGTTCA CAAGCGTGGC 
ATCTACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCCAAC 
TTTACAATAG CAATGGTTGT GGCCCCAGAA CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTGGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTACGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTGTTGGTTA TTTTCTTCGT 
GCAAAACTAT ATTTTTCCAA ATTGATGGAT CGGGAGACTA ATGCAAGGAC TATATTTTTG 
GTTAAAAATG TTCTTTCTCG GCATTATGTT CATCTTGAGA GGTCCCCTTG GAAAGGACTC 
CCAGAACTGA CCAACGAGAA TGGCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA AACACTTTAT GATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_008263969.2 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_008262191.1 Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.

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CloneID	OOb13095
Clone ID Related Accession (Same CDS sequence)	XM_008263968.2 , XM_008263966.2 , XM_008263969.2 , XM_017345486.1
Accession Version	XM_008263969.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2016 this sequence version replaced XM_008263969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAG CTACAATTTC GATTAGGCCC AACTTTACAG 
GGAAAACCAG TTACTGTATT TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGAGAA 
AAATTCCGGT CCCTGGAGTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGCAAACTCA ATCTGCAACA GTCTGGTTCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATGTTACACT TGGACTGTGT CACTCTCCAG ACATTTTTAG CCAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GTTACAACAT GATCCATTTT 
ACCCCACTGC AGACTCTTGG ACTGTCTAGG TCGTGCTACT CCCTTGCTGA CCAATTAGAA 
TTAAATCCCG ACTTCTCAAG GCCTCATAAA AAATACACGT GGAGTGATGT GGGACAGTTA 
GTGGAAAAAC TGAAAAGGGA ATGGAATGTT CTTTGCATTA CAGATGTTGT CTACAATCAT 
ACCGCTGCTA ATAGTAAATG GATTCAGGAA CATCCAGAAT GTGCATATAA TCTTGTGAAC 
TCACCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT CTCCTGTGAT 
GTTGCGGAAG GAAAATACAA AAACAGAGGA GTACCTGCTT TGATTGAGAA TGATCATCAT 
TTGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGCTTCA TCTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGAAATTTA GAGGACTTCT AACACAAGAA 
ACTTGGAGGG TAATCAAGTC AGATCCAAAA CAACATCTCA AGATTATCCA GGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAGCAACTTT CATACCACAC 
GACAATGGGC CAGCTGCAAT TGAAGAATGC TGTAACTGGT TCCGGAAGAG AATTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTCATGAAC TATCATCAGG AACAGGCAGT TAACTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CTAAATTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGGTACTTT ACTTTTCCAT TTGAGGAAAT GCCTGTATCT 
ACAGAAGAAA CTATGATTCA CCTCCCAAAC AAAGCTTGTT TCTTCATGGC ACATAATGGT 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CAGGTTCAGA TGTTTATTTA 
AGAAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGAAC TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAGA AAATACACTG AAATAATTGC AACTTATTTC 
CAAGGAGTAC GTCTTGATAA TTGCCATTCA ACACCTCTTC ATGTAGCCGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAACATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAAGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CGGCCATCGC ACACGCTCTG 
TTTATGGATA TTACCCATGA TAATGAGTGC CCTATTGTGC ATAGATCAGT GTACGATGCT 
CTTCCAAGTA CCACAATTGT TTCTATGGCT TGTTGTGCTA GTGGAAGTAC AAGGGGCTAT 
GATGAATTAG TGCCACATCA GATTTCAGTG GTTTCTGAAG AACGTTTTTA CACTAAATGG 
AACCCTGAAG CCTTGCCATC AAATGCTGGT GAAGTTAATT TCCAAAGTGG AATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAA GAGCTTGGAG CCAAAGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGATATAGTG GCAGTAACAA GACACTCACC AAGTATCCAT 
CAGTCTGTTG TGGCCGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGATGTGC CTCAAATGTG CATCCCTGGT AAAATTGAAG AAGTAGTTCT TGAAGCTCGA 
ACTATTGAGA GAAATATAAG TCCTTACAGG AAGGATGAGA ATTCGATAAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAGAAT TGTTAAACAA 
GCTGGAGTTA CTACAAAAGG TCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTGCTCACT TTAAAGCAGG AAGCCTTGCT 
GTTGACAACT CAGATCCAAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAAATTAACT 
TTAGCTGAGA TAAATCAGAT ACTTTACCGG TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGATGCTATG ACATACCAAA CTGGTCTTCC CTTAAATATG CAGGTCTGCA AGGGTTAATG 
TCCGTATTGG CAGAAATACG ACCAAAGAAT GACTTGGGAC ACCCTTTCTG TGATAATTTG 
CGGTCTGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TATCTTATAC CATGTTACTT TGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAAATGTC AAGTTTTGTT CAGACTGGTT CAACCTTTGT CAAACACCTC 
TCTCTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAAATTCC CTTCTCTGCC ACTTCTTTCA 
CCTTCACTTA CGGATGTCCC ATATAGGCTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTTCG CTGTTGGGGA 
AGAGACACTT TTATTGCACT TAGAGGTTTG CTGCTGATTA CTGGACGCTA CTTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCTGGTACC CTGAGGCATG GTCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTTATGCCAG ATACAATTGC CGTGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TCCTCAAGTG CCCAGTCTCC 
AGAATGTATC CTACAGATGA TTCTGCCCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGATGTAA TACAGGAGGC TATGCAAAGA CACATGCAGG GCATCCAGTT TCGGGAAAGG 
AATGCTGGTC CACAAATAGA CCGAAACATG AAGGATGAAG GTTTTACTGT AATTGCTGGG 
GTTAATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGTGG TACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCGAGA AACAGAGGGA TCCCAGCCAC TCCCAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTATGT AAATCTACAG TTCGTTGGTT GCTGGAGTTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCAGAGTAA AAAGACATGG AAAGGTGGTA 
ACGGTCTCAT ATGAGGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCGGTTTCAT 
GTTTCAGAAG ATCCTTCAGC ATCCAATGAA GAACATCCAA ATCTGGTTCA CAAGCGTGGC 
ATCTACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCCAAC 
TTTACAATAG CAATGGTTGT GGCCCCAGAA CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTGGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTACGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTGTTGGTTA TTTTCTTCGT 
GCAAAACTAT ATTTTTCCAA ATTGATGGAT CGGGAGACTA ATGCAAGGAC TATATTTTTG 
GTTAAAAATG TTCTTTCTCG GCATTATGTT CATCTTGAGA GGTCCCCTTG GAAAGGACTC 
CCAGAACTGA CCAACGAGAA TGGCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA AACACTTTAT GATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008262191.1
CDS	316..4914
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKPVTVFTNYPFPGETFNREKFRSLEWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHMLHLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPHKKYTWSDVGQLVEKLKREWNVLCITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWHFSCDVAEGKYKNRGVPALIENDHHLNCIRKVIWEDIFPKLHLWEFFQVDVYKAVEKFRGLLTQETWRVIKSDPKQHLKIIQDPEYRRFGCTVDMNIALATFIPHDNGPAAIEECCNWFRKRIEELNSEKHQLMNYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKYPLVTRYFTFPFEEMPVSTEETMIHLPNKACFFMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGTKPEDCPYLWAHMRKYTEIIATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSVYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNAGEVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKDVPQMCIPGKIEEVVLEARTIERNISPYRKDENSINGMPNITVEIREHIQLNESRIVKQAGVTTKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSAHFKAGSLAVDNSDPILKIPFASIASKLTLAEINQILYRCESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSGRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQTGSTFVKHLSLGSVQMCGVGKFPSLPLLSPSLTDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFAGTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFDVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFTVIAGVNEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLCKSTVRWLLELSKKNIFPYHEVRVKRHGKVVTVSYEEWNRKIQDNFEKRFHVSEDPSASNEEHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSKLMDRETNARTIFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_008263969.2
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008263969.2

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAG CTACAATTTC GATTAGGCCC AACTTTACAG 
GGAAAACCAG TTACTGTATT TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGAGAA 
AAATTCCGGT CCCTGGAGTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGCAAACTCA ATCTGCAACA GTCTGGTTCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATGTTACACT TGGACTGTGT CACTCTCCAG ACATTTTTAG CCAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GTTACAACAT GATCCATTTT 
ACCCCACTGC AGACTCTTGG ACTGTCTAGG TCGTGCTACT CCCTTGCTGA CCAATTAGAA 
TTAAATCCCG ACTTCTCAAG GCCTCATAAA AAATACACGT GGAGTGATGT GGGACAGTTA 
GTGGAAAAAC TGAAAAGGGA ATGGAATGTT CTTTGCATTA CAGATGTTGT CTACAATCAT 
ACCGCTGCTA ATAGTAAATG GATTCAGGAA CATCCAGAAT GTGCATATAA TCTTGTGAAC 
TCACCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT CTCCTGTGAT 
GTTGCGGAAG GAAAATACAA AAACAGAGGA GTACCTGCTT TGATTGAGAA TGATCATCAT 
TTGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGCTTCA TCTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGAAATTTA GAGGACTTCT AACACAAGAA 
ACTTGGAGGG TAATCAAGTC AGATCCAAAA CAACATCTCA AGATTATCCA GGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAGCAACTTT CATACCACAC 
GACAATGGGC CAGCTGCAAT TGAAGAATGC TGTAACTGGT TCCGGAAGAG AATTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTCATGAAC TATCATCAGG AACAGGCAGT TAACTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CTAAATTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGGTACTTT ACTTTTCCAT TTGAGGAAAT GCCTGTATCT 
ACAGAAGAAA CTATGATTCA CCTCCCAAAC AAAGCTTGTT TCTTCATGGC ACATAATGGT 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CAGGTTCAGA TGTTTATTTA 
AGAAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGAAC TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAGA AAATACACTG AAATAATTGC AACTTATTTC 
CAAGGAGTAC GTCTTGATAA TTGCCATTCA ACACCTCTTC ATGTAGCCGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAACATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAAGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CGGCCATCGC ACACGCTCTG 
TTTATGGATA TTACCCATGA TAATGAGTGC CCTATTGTGC ATAGATCAGT GTACGATGCT 
CTTCCAAGTA CCACAATTGT TTCTATGGCT TGTTGTGCTA GTGGAAGTAC AAGGGGCTAT 
GATGAATTAG TGCCACATCA GATTTCAGTG GTTTCTGAAG AACGTTTTTA CACTAAATGG 
AACCCTGAAG CCTTGCCATC AAATGCTGGT GAAGTTAATT TCCAAAGTGG AATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAA GAGCTTGGAG CCAAAGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGATATAGTG GCAGTAACAA GACACTCACC AAGTATCCAT 
CAGTCTGTTG TGGCCGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGATGTGC CTCAAATGTG CATCCCTGGT AAAATTGAAG AAGTAGTTCT TGAAGCTCGA 
ACTATTGAGA GAAATATAAG TCCTTACAGG AAGGATGAGA ATTCGATAAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAGAAT TGTTAAACAA 
GCTGGAGTTA CTACAAAAGG TCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTGCTCACT TTAAAGCAGG AAGCCTTGCT 
GTTGACAACT CAGATCCAAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAAATTAACT 
TTAGCTGAGA TAAATCAGAT ACTTTACCGG TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGATGCTATG ACATACCAAA CTGGTCTTCC CTTAAATATG CAGGTCTGCA AGGGTTAATG 
TCCGTATTGG CAGAAATACG ACCAAAGAAT GACTTGGGAC ACCCTTTCTG TGATAATTTG 
CGGTCTGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TATCTTATAC CATGTTACTT TGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAAATGTC AAGTTTTGTT CAGACTGGTT CAACCTTTGT CAAACACCTC 
TCTCTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAAATTCC CTTCTCTGCC ACTTCTTTCA 
CCTTCACTTA CGGATGTCCC ATATAGGCTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTTCG CTGTTGGGGA 
AGAGACACTT TTATTGCACT TAGAGGTTTG CTGCTGATTA CTGGACGCTA CTTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCTGGTACC CTGAGGCATG GTCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTTATGCCAG ATACAATTGC CGTGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TCCTCAAGTG CCCAGTCTCC 
AGAATGTATC CTACAGATGA TTCTGCCCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGATGTAA TACAGGAGGC TATGCAAAGA CACATGCAGG GCATCCAGTT TCGGGAAAGG 
AATGCTGGTC CACAAATAGA CCGAAACATG AAGGATGAAG GTTTTACTGT AATTGCTGGG 
GTTAATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGTGG TACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCGAGA AACAGAGGGA TCCCAGCCAC TCCCAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTATGT AAATCTACAG TTCGTTGGTT GCTGGAGTTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCAGAGTAA AAAGACATGG AAAGGTGGTA 
ACGGTCTCAT ATGAGGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCGGTTTCAT 
GTTTCAGAAG ATCCTTCAGC ATCCAATGAA GAACATCCAA ATCTGGTTCA CAAGCGTGGC 
ATCTACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCCAAC 
TTTACAATAG CAATGGTTGT GGCCCCAGAA CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTGGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTACGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTGTTGGTTA TTTTCTTCGT 
GCAAAACTAT ATTTTTCCAA ATTGATGGAT CGGGAGACTA ATGCAAGGAC TATATTTTTG 
GTTAAAAATG TTCTTTCTCG GCATTATGTT CATCTTGAGA GGTCCCCTTG GAAAGGACTC 
CCAGAACTGA CCAACGAGAA TGGCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA AACACTTTAT GATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_017345486.1 ) cDNA ORF clone, Oryctolagus cuniculus(rabbit) -> XP_017200975.1 Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

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CloneID	OOb13095
Clone ID Related Accession (Same CDS sequence)	XM_008263968.2 , XM_008263966.2 , XM_008263969.2 , XM_017345486.1
Accession Version	XM_017345486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466524800000
Organism	Oryctolagus cuniculus(rabbit)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_013681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oryctolagus cuniculus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAG CTACAATTTC GATTAGGCCC AACTTTACAG 
GGAAAACCAG TTACTGTATT TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGAGAA 
AAATTCCGGT CCCTGGAGTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGCAAACTCA ATCTGCAACA GTCTGGTTCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATGTTACACT TGGACTGTGT CACTCTCCAG ACATTTTTAG CCAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GTTACAACAT GATCCATTTT 
ACCCCACTGC AGACTCTTGG ACTGTCTAGG TCGTGCTACT CCCTTGCTGA CCAATTAGAA 
TTAAATCCCG ACTTCTCAAG GCCTCATAAA AAATACACGT GGAGTGATGT GGGACAGTTA 
GTGGAAAAAC TGAAAAGGGA ATGGAATGTT CTTTGCATTA CAGATGTTGT CTACAATCAT 
ACCGCTGCTA ATAGTAAATG GATTCAGGAA CATCCAGAAT GTGCATATAA TCTTGTGAAC 
TCACCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT CTCCTGTGAT 
GTTGCGGAAG GAAAATACAA AAACAGAGGA GTACCTGCTT TGATTGAGAA TGATCATCAT 
TTGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGCTTCA TCTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGAAATTTA GAGGACTTCT AACACAAGAA 
ACTTGGAGGG TAATCAAGTC AGATCCAAAA CAACATCTCA AGATTATCCA GGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAGCAACTTT CATACCACAC 
GACAATGGGC CAGCTGCAAT TGAAGAATGC TGTAACTGGT TCCGGAAGAG AATTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTCATGAAC TATCATCAGG AACAGGCAGT TAACTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CTAAATTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGGTACTTT ACTTTTCCAT TTGAGGAAAT GCCTGTATCT 
ACAGAAGAAA CTATGATTCA CCTCCCAAAC AAAGCTTGTT TCTTCATGGC ACATAATGGT 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CAGGTTCAGA TGTTTATTTA 
AGAAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGAAC TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAGA AAATACACTG AAATAATTGC AACTTATTTC 
CAAGGAGTAC GTCTTGATAA TTGCCATTCA ACACCTCTTC ATGTAGCCGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAACATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAAGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CGGCCATCGC ACACGCTCTG 
TTTATGGATA TTACCCATGA TAATGAGTGC CCTATTGTGC ATAGATCAGT GTACGATGCT 
CTTCCAAGTA CCACAATTGT TTCTATGGCT TGTTGTGCTA GTGGAAGTAC AAGGGGCTAT 
GATGAATTAG TGCCACATCA GATTTCAGTG GTTTCTGAAG AACGTTTTTA CACTAAATGG 
AACCCTGAAG CCTTGCCATC AAATGCTGGT GAAGTTAATT TCCAAAGTGG AATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAA GAGCTTGGAG CCAAAGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGATATAGTG GCAGTAACAA GACACTCACC AAGTATCCAT 
CAGTCTGTTG TGGCCGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGATGTGC CTCAAATGTG CATCCCTGGT AAAATTGAAG AAGTAGTTCT TGAAGCTCGA 
ACTATTGAGA GAAATATAAG TCCTTACAGG AAGGATGAGA ATTCGATAAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAGAAT TGTTAAACAA 
GCTGGAGTTA CTACAAAAGG TCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTGCTCACT TTAAAGCAGG AAGCCTTGCT 
GTTGACAACT CAGATCCAAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAAATTAACT 
TTAGCTGAGA TAAATCAGAT ACTTTACCGG TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGATGCTATG ACATACCAAA CTGGTCTTCC CTTAAATATG CAGGTCTGCA AGGGTTAATG 
TCCGTATTGG CAGAAATACG ACCAAAGAAT GACTTGGGAC ACCCTTTCTG TGATAATTTG 
CGGTCTGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TATCTTATAC CATGTTACTT TGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAAATGTC AAGTTTTGTT CAGACTGGTT CAACCTTTGT CAAACACCTC 
TCTCTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAAATTCC CTTCTCTGCC ACTTCTTTCA 
CCTTCACTTA CGGATGTCCC ATATAGGCTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTTCG CTGTTGGGGA 
AGAGACACTT TTATTGCACT TAGAGGTTTG CTGCTGATTA CTGGACGCTA CTTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCTGGTACC CTGAGGCATG GTCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTTATGCCAG ATACAATTGC CGTGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TCCTCAAGTG CCCAGTCTCC 
AGAATGTATC CTACAGATGA TTCTGCCCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGATGTAA TACAGGAGGC TATGCAAAGA CACATGCAGG GCATCCAGTT TCGGGAAAGG 
AATGCTGGTC CACAAATAGA CCGAAACATG AAGGATGAAG GTTTTACTGT AATTGCTGGG 
GTTAATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGTGG TACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCGAGA AACAGAGGGA TCCCAGCCAC TCCCAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTATGT AAATCTACAG TTCGTTGGTT GCTGGAGTTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCAGAGTAA AAAGACATGG AAAGGTGGTA 
ACGGTCTCAT ATGAGGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCGGTTTCAT 
GTTTCAGAAG ATCCTTCAGC ATCCAATGAA GAACATCCAA ATCTGGTTCA CAAGCGTGGC 
ATCTACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCCAAC 
TTTACAATAG CAATGGTTGT GGCCCCAGAA CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTGGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTACGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTGTTGGTTA TTTTCTTCGT 
GCAAAACTAT ATTTTTCCAA ATTGATGGAT CGGGAGACTA ATGCAAGGAC TATATTTTTG 
GTTAAAAATG TTCTTTCTCG GCATTATGTT CATCTTGAGA GGTCCCCTTG GAAAGGACTC 
CCAGAACTGA CCAACGAGAA TGGCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA AACACTTTAT GATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017200975.1
CDS	335..4933
Translation	MGHSKQIRILLLNEMEKLEKTLFRLEQGFELQFRLGPTLQGKPVTVFTNYPFPGETFNREKFRSLEWENPTEREDDSDKYCKLNLQQSGSFQYYFLQGNEKSGGGYIVVDPILRVGADNHMLHLDCVTLQTFLAKCLGPFDEWESRLRVAKESGYNMIHFTPLQTLGLSRSCYSLADQLELNPDFSRPHKKYTWSDVGQLVEKLKREWNVLCITDVVYNHTAANSKWIQEHPECAYNLVNSPHLKPAWVLDRALWHFSCDVAEGKYKNRGVPALIENDHHLNCIRKVIWEDIFPKLHLWEFFQVDVYKAVEKFRGLLTQETWRVIKSDPKQHLKIIQDPEYRRFGCTVDMNIALATFIPHDNGPAAIEECCNWFRKRIEELNSEKHQLMNYHQEQAVNCLLGNVFYERLAGHGPKLGPVTRKYPLVTRYFTFPFEEMPVSTEETMIHLPNKACFFMAHNGWVMGDDPLRNFAEPGSDVYLRRELICWGDSVKLRYGTKPEDCPYLWAHMRKYTEIIATYFQGVRLDNCHSTPLHVAEYMLDAARKLQPNLYVVAELFTGSEDLDNIFVTRLGISSLIREAMSAYNSHEEGRLVYRYGGEPVGSFVQPCLRPLMPAIAHALFMDITHDNECPIVHRSVYDALPSTTIVSMACCASGSTRGYDELVPHQISVVSEERFYTKWNPEALPSNAGEVNFQSGIIAARCAINKLHQELGAKGFIQVYVDQVDEDIVAVTRHSPSIHQSVVAVSRTAFRNPKTSFYSKDVPQMCIPGKIEEVVLEARTIERNISPYRKDENSINGMPNITVEIREHIQLNESRIVKQAGVTTKGPNEYIQEIEFENLSPGSVIIFRVSLDPHAQVAVGILRNHLTQFSAHFKAGSLAVDNSDPILKIPFASIASKLTLAEINQILYRCESEEQEDGGGCYDIPNWSSLKYAGLQGLMSVLAEIRPKNDLGHPFCDNLRSGDWMIDYVSGRLISRSGTIAEVGKWLQAMFFYLKQIPRYLIPCYFDAILIGAYTTLLDIAWKQMSSFVQTGSTFVKHLSLGSVQMCGVGKFPSLPLLSPSLTDVPYRLNEITKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFIALRGLLLITGRYLEARNIILAFAGTLRHGLIPNLLGEGTYARYNCRDAVWWWLQCIQDYCKMVPNGLDILKCPVSRMYPTDDSAPLPAGTLDQPLFDVIQEAMQRHMQGIQFRERNAGPQIDRNMKDEGFTVIAGVNEETGFVYGGNRFNCGTWMDKMGESDRARNRGIPATPRDGSAVEIVGLCKSTVRWLLELSKKNIFPYHEVRVKRHGKVVTVSYEEWNRKIQDNFEKRFHVSEDPSASNEEHPNLVHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPELFTAEKAWKALEIAEKKLLGPLGMKTLDPDDMVYCGIYDNALDNDNYNLAKGFNYHQGPEWLWPVGYFLRAKLYFSKLMDRETNARTIFLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATILETLYDL

Target ORF information:

RefSeq Version	XM_017345486.1
Organism	Oryctolagus cuniculus(rabbit)
Definition	Oryctolagus cuniculus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017345486.1

ORF Insert Sequence:

ATGGGACACA GTAAACAGAT TCGAATTTTA CTACTGAACG AAATGGAAAA GCTGGAAAAG 
ACCCTCTTCA GACTTGAACA AGGATTTGAG CTACAATTTC GATTAGGCCC AACTTTACAG 
GGAAAACCAG TTACTGTATT TACAAATTAT CCATTTCCTG GAGAAACATT TAATCGAGAA 
AAATTCCGGT CCCTGGAGTG GGAAAATCCA ACAGAAAGAG AAGATGATTC TGATAAATAC 
TGCAAACTCA ATCTGCAACA GTCTGGTTCA TTTCAGTATT ACTTCCTTCA AGGAAATGAG 
AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTAC GTGTTGGTGC TGATAATCAC 
ATGTTACACT TGGACTGTGT CACTCTCCAG ACATTTTTAG CCAAGTGTTT GGGACCCTTT 
GATGAATGGG AAAGCAGACT TAGGGTTGCA AAAGAATCAG GTTACAACAT GATCCATTTT 
ACCCCACTGC AGACTCTTGG ACTGTCTAGG TCGTGCTACT CCCTTGCTGA CCAATTAGAA 
TTAAATCCCG ACTTCTCAAG GCCTCATAAA AAATACACGT GGAGTGATGT GGGACAGTTA 
GTGGAAAAAC TGAAAAGGGA ATGGAATGTT CTTTGCATTA CAGATGTTGT CTACAATCAT 
ACCGCTGCTA ATAGTAAATG GATTCAGGAA CATCCAGAAT GTGCATATAA TCTTGTGAAC 
TCACCACACT TAAAACCTGC CTGGGTCTTA GACAGAGCAC TGTGGCATTT CTCCTGTGAT 
GTTGCGGAAG GAAAATACAA AAACAGAGGA GTACCTGCTT TGATTGAGAA TGATCATCAT 
TTGAATTGCA TTCGAAAAGT AATTTGGGAG GATATTTTTC CAAAGCTTCA TCTCTGGGAA 
TTTTTCCAAG TAGACGTTTA CAAAGCAGTT GAGAAATTTA GAGGACTTCT AACACAAGAA 
ACTTGGAGGG TAATCAAGTC AGATCCAAAA CAACATCTCA AGATTATCCA GGATCCTGAA 
TACAGACGGT TTGGCTGTAC TGTAGATATG AACATTGCAC TAGCAACTTT CATACCACAC 
GACAATGGGC CAGCTGCAAT TGAAGAATGC TGTAACTGGT TCCGGAAGAG AATTGAGGAA 
TTAAATTCAG AGAAGCATCA ACTCATGAAC TATCATCAGG AACAGGCAGT TAACTGTCTT 
TTGGGAAATG TGTTTTATGA ACGACTGGCT GGCCATGGTC CTAAATTAGG ACCTGTCACT 
AGAAAATATC CTTTAGTTAC CAGGTACTTT ACTTTTCCAT TTGAGGAAAT GCCTGTATCT 
ACAGAAGAAA CTATGATTCA CCTCCCAAAC AAAGCTTGTT TCTTCATGGC ACATAATGGT 
TGGGTAATGG GAGATGATCC TCTTCGAAAC TTTGCTGAAC CAGGTTCAGA TGTTTATTTA 
AGAAGAGAAC TTATTTGCTG GGGAGACAGT GTTAAATTAC GCTATGGAAC TAAACCAGAG 
GACTGTCCTT ATCTCTGGGC ACACATGAGA AAATACACTG AAATAATTGC AACTTATTTC 
CAAGGAGTAC GTCTTGATAA TTGCCATTCA ACACCTCTTC ATGTAGCCGA GTACATGTTG 
GATGCTGCTA GGAAATTGCA ACCCAATTTA TATGTAGTAG CCGAACTGTT CACAGGAAGT 
GAAGATCTGG ACAACATCTT TGTTACCAGA CTGGGCATTA GTTCCTTAAT AAGAGAGGCA 
ATGAGTGCAT ATAATAGTCA TGAAGAAGGC AGATTAGTTT ACCGATATGG AGGAGAACCT 
GTTGGATCCT TTGTTCAGCC CTGTTTGAGG CCTTTAATGC CGGCCATCGC ACACGCTCTG 
TTTATGGATA TTACCCATGA TAATGAGTGC CCTATTGTGC ATAGATCAGT GTACGATGCT 
CTTCCAAGTA CCACAATTGT TTCTATGGCT TGTTGTGCTA GTGGAAGTAC AAGGGGCTAT 
GATGAATTAG TGCCACATCA GATTTCAGTG GTTTCTGAAG AACGTTTTTA CACTAAATGG 
AACCCTGAAG CCTTGCCATC AAATGCTGGT GAAGTTAATT TCCAAAGTGG AATTATTGCA 
GCCAGGTGTG CTATCAATAA ACTTCATCAA GAGCTTGGAG CCAAAGGTTT TATTCAGGTG 
TATGTGGATC AAGTTGATGA AGATATAGTG GCAGTAACAA GACACTCACC AAGTATCCAT 
CAGTCTGTTG TGGCCGTATC TAGAACTGCT TTCAGGAATC CCAAGACTTC ATTTTACAGC 
AAGGATGTGC CTCAAATGTG CATCCCTGGT AAAATTGAAG AAGTAGTTCT TGAAGCTCGA 
ACTATTGAGA GAAATATAAG TCCTTACAGG AAGGATGAGA ATTCGATAAA TGGAATGCCA 
AATATCACAG TAGAAATTAG AGAACATATT CAGCTTAATG AAAGTAGAAT TGTTAAACAA 
GCTGGAGTTA CTACAAAAGG TCCCAATGAA TATATTCAAG AAATAGAATT TGAAAACTTG 
TCTCCAGGAA GTGTTATTAT ATTCAGAGTT AGTCTTGATC CACATGCACA AGTTGCTGTT 
GGAATTCTTC GAAATCATCT GACACAGTTC AGTGCTCACT TTAAAGCAGG AAGCCTTGCT 
GTTGACAACT CAGATCCAAT ATTAAAAATT CCTTTTGCTT CTATTGCCTC CAAATTAACT 
TTAGCTGAGA TAAATCAGAT ACTTTACCGG TGTGAATCAG AAGAACAAGA AGATGGTGGA 
GGATGCTATG ACATACCAAA CTGGTCTTCC CTTAAATATG CAGGTCTGCA AGGGTTAATG 
TCCGTATTGG CAGAAATACG ACCAAAGAAT GACTTGGGAC ACCCTTTCTG TGATAATTTG 
CGGTCTGGAG ATTGGATGAT TGACTATGTC AGTGGCCGGC TTATTTCACG ATCAGGAACT 
ATTGCTGAAG TTGGTAAATG GTTGCAGGCT ATGTTCTTCT ACCTGAAGCA GATCCCACGT 
TATCTTATAC CATGTTACTT TGATGCTATA TTAATTGGTG CATACACCAC TCTTCTGGAT 
ATAGCATGGA AGCAAATGTC AAGTTTTGTT CAGACTGGTT CAACCTTTGT CAAACACCTC 
TCTCTGGGTT CAGTCCAAAT GTGTGGAGTA GGAAAATTCC CTTCTCTGCC ACTTCTTTCA 
CCTTCACTTA CGGATGTCCC ATATAGGCTA AATGAGATCA CAAAAGAAAA GGAGCAATGT 
TGTGTTTCTC TGGCTGCAGG CTTACCTCAT TTTTCTTCTG GTATTTTTCG CTGTTGGGGA 
AGAGACACTT TTATTGCACT TAGAGGTTTG CTGCTGATTA CTGGACGCTA CTTAGAAGCC 
AGGAATATTA TTTTAGCATT TGCTGGTACC CTGAGGCATG GTCTCATTCC TAATCTCCTG 
GGTGAAGGAA CTTATGCCAG ATACAATTGC CGTGATGCTG TGTGGTGGTG GCTACAGTGT 
ATTCAGGATT ACTGTAAAAT GGTTCCAAAT GGTCTAGACA TCCTCAAGTG CCCAGTCTCC 
AGAATGTATC CTACAGATGA TTCTGCCCCT TTGCCTGCTG GCACACTGGA TCAGCCATTG 
TTTGATGTAA TACAGGAGGC TATGCAAAGA CACATGCAGG GCATCCAGTT TCGGGAAAGG 
AATGCTGGTC CACAAATAGA CCGAAACATG AAGGATGAAG GTTTTACTGT AATTGCTGGG 
GTTAATGAAG AAACAGGATT TGTTTATGGA GGAAATCGCT TCAATTGTGG TACGTGGATG 
GATAAAATGG GAGAAAGTGA CAGAGCGAGA AACAGAGGGA TCCCAGCCAC TCCCAGAGAT 
GGGTCTGCTG TGGAAATTGT GGGCCTATGT AAATCTACAG TTCGTTGGTT GCTGGAGTTA 
TCCAAAAAAA ATATTTTCCC TTATCATGAA GTCAGAGTAA AAAGACATGG AAAGGTGGTA 
ACGGTCTCAT ATGAGGAGTG GAACAGGAAA ATACAAGACA ACTTTGAAAA GCGGTTTCAT 
GTTTCAGAAG ATCCTTCAGC ATCCAATGAA GAACATCCAA ATCTGGTTCA CAAGCGTGGC 
ATCTACAAAG ATAGTTACGG AGCTTCAAGT CCTTGGTGTG ACTACCAGCT CAGGCCCAAC 
TTTACAATAG CAATGGTTGT GGCCCCAGAA CTCTTTACTG CAGAAAAAGC ATGGAAAGCT 
TTGGAGATTG CAGAAAAAAA ATTGCTTGGT CCCCTGGGCA TGAAAACTTT AGATCCAGAT 
GATATGGTTT ACTGTGGAAT TTACGACAAT GCCTTAGACA ATGACAACTA TAATCTTGCT 
AAAGGTTTCA ATTATCACCA AGGACCTGAG TGGCTGTGGC CTGTTGGTTA TTTTCTTCGT 
GCAAAACTAT ATTTTTCCAA ATTGATGGAT CGGGAGACTA ATGCAAGGAC TATATTTTTG 
GTTAAAAATG TTCTTTCTCG GCATTATGTT CATCTTGAGA GGTCCCCTTG GAAAGGACTC 
CCAGAACTGA CCAACGAGAA TGGCCAGTAC TGTCCTTTCA GCTGTGAAAC ACAAGCCTGG 
TCAATTGCTA CTATTCTTGA AACACTTTAT GATTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular cloning, sequencing, and analysis of the cDNA for rabbit muscle glycogen debranching enzyme.
Archives of biochemistry and biophysics306(1)232-9(1993 Oct)
Liu W,de Castro ML,Takrama J,Bilous PT,Vinayagamoorthy T,Madsen NB,Bleackley RC

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AGL cDNA ORF clone, Oryctolagus cuniculus(rabbit)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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