| Gene Symbol | PKM |
| Entrez Gene ID | 100008676 |
| Full Name | pyruvate kinase M1/2 |
| Synonyms | PKM2 |
| Gene Type | protein-coding |
| Organism | Oryctolagus cuniculus(rabbit) |
IVD Raw Materials
ORF » Species Summary » Oryctolagus cuniculus » PKM cDNA ORF clone
| Gene Symbol | PKM |
| Entrez Gene ID | 100008676 |
| Full Name | pyruvate kinase M1/2 |
| Synonyms | PKM2 |
| Gene Type | protein-coding |
| Organism | Oryctolagus cuniculus(rabbit) |
| mRNA | Protein | Name |
|---|---|---|
| NM_001195644.1 | NP_001182573.1 | pyruvate kinase PKM isoform 1 |
| NM_001195645.1 | NP_001182574.1 | pyruvate kinase PKM isoform 2 |
[Different properties of pyruvate kinase from rabbit and hare muscles].
Strumilo S, Tylicki A
Zhurnal evoliutsionnoi biokhimii i fiziologii51(2)103-7(2015 Mar-Apr)
The importance of polarity in the evolution of the K+ binding site of pyruvate kinase.
Ramírez-Silva L, Guerrero-Mendiola C, Cabrera N
International journal of molecular sciences15(12)22214-26(2014 Dec)
Identification of regions of rabbit muscle pyruvate kinase important for allosteric regulation by phenylalanine, detected by H/D exchange mass spectrometry.
Prasannan CB, Villar MT, Artigues A, Fenton AW
Biochemistry52(11)1998-2006(2013 Mar)
Distinguishing the chemical moiety of phosphoenolpyruvate that contributes to allostery in muscle pyruvate kinase.
Urness JM, Clapp KM, Timmons JC, Bai X, Chandrasoma N, Buszek KR, Fenton AW
Biochemistry52(1)1-3(2013 Jan)
Effects of ions on ligand binding to pyruvate kinase: mapping the binding site with infrared spectroscopy.
Kumar S, Barth A
The journal of physical chemistry. B115(20)6784-9(2011 May)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The following PKM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PKM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OOb00318 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001195644.1 | |
| Accession Version | NM_001195644.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 1568908800000 | |
| Organism | Oryctolagus cuniculus(rabbit) | |
| Product | pyruvate kinase PKM isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAGW02071942.1 and U44751.1. On Sep 23, 2010 this sequence version replaced XM_002717702.2. Transcript Variant: This variant (1) uses an alternate in-frame exon in the 3' coding region compared to variant 2. The resulting protein (isoform 1) has a different internal segment but is the same length as isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U44751.1, GBCA01044233.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1876818, SAMEA1876819 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## | |
1 | ATGTCGAAGT CCCACAGTGA AGCCGGGTCT GCCTTCATCC AGACGCAGCA GCTGCACGCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001182573.1 |
| CDS | 1..1596 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001195644.1 |
1 | ATGTCGAAGT CCCACAGTGA AGCCGGGTCT GCCTTCATCC AGACGCAGCA GCTGCACGCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OOb00322 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001195645.1 | |
| Accession Version | NM_001195645.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 1568908800000 | |
| Organism | Oryctolagus cuniculus(rabbit) | |
| Product | pyruvate kinase PKM isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAGW02071942.1 and AF032389.1. Transcript Variant: This variant (2) uses an alternate in-frame exon in the 3' coding region compared to variant 1. The resulting protein (isoform 2) has a different internal segment but is the same length as isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF032389.1, GBCA01044234.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1876818, SAMEA1876819 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## | |
1 | ATGTCGAAGT CCCACAGTGA AGCCGGGTCT GCCTTCATCC AGACGCAGCA GCTGCACGCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001182574.1 |
| CDS | 1..1596 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001195645.1 |
1 | ATGTCGAAGT CCCACAGTGA AGCCGGGTCT GCCTTCATCC AGACGCAGCA GCTGCACGCA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
[Different properties of pyruvate kinase from rabbit and hare muscles]. |
The importance of polarity in the evolution of the K+ binding site of pyruvate kinase. |
Identification of regions of rabbit muscle pyruvate kinase important for allosteric regulation by phenylalanine, detected by H/D exchange mass spectrometry. |
Distinguishing the chemical moiety of phosphoenolpyruvate that contributes to allostery in muscle pyruvate kinase. |
Effects of ions on ligand binding to pyruvate kinase: mapping the binding site with infrared spectroscopy. |