SPTBN1 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPTBN1
Entrez Gene ID	100078052
Full Name	spectrin beta, non-erythrocytic 1
Gene Type	protein-coding
Organism	Ornithorhynchus anatinus(platypus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029071831.1	XP_028927664.1	spectrin beta chain, non-erythrocytic 1 isoform X2
XM_003431278.4	XP_003431326.2	spectrin beta chain, non-erythrocytic 1 isoform X1
XM_003431278.3	XP_003431326.1	LOW QUALITY PROTEIN: spectrin beta chain, non-erythrocytic 1
XM_029071832.1	XP_028927665.1	spectrin beta chain, non-erythrocytic 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following SPTBN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOc134130	XM_003431278.4 Latest version!	Ornithorhynchus anatinus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OOc134131	XM_029071831.1 Latest version!	Ornithorhynchus anatinus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OOc134132	XM_029071832.1 Latest version!	Ornithorhynchus anatinus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OOc13690	XM_003431278.3 Latest version!	Ornithorhynchus anatinus spectrin beta, non-erythrocytic 1 (SPTBN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPTBN1 ( XM_003431278.4 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_003431326.2 Ornithorhynchus anatinus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOc134130
Clone ID Related Accession (Same CDS sequence)	XM_003431278.4
Accession Version	XM_003431278.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	spectrin beta chain, non-erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041736.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 29, 2019 this sequence version replaced XM_003431278.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACGA CGGTGGCCAC AGATTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAC 
GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAAA ACAGCTCCGC CCGGCTCTTT 
GAGCGATCCC GAATCAAGGC GCTAGCAGAT GAGCGGGAAG CAGTGCAGAA GAAGACGTTC 
ACCAAATGGG TCAACTCACA CCTGGCTCGG GTGTCTTGCC GAATCACCGA CTTGTACGCC 
GACCTGCGCG ATGGACGGAT GCTCATCAAA TTGCTCGAGG TTCTGTCCGG AGAGAGACTC 
CCCAAACCAA CCAAAGGAAG AATGCGTATC CACTGCCTGG AGAATGTGGA CAAAGCCCTC 
CAGTTCTTGA AGGAGCAGCG AGTGCACCTG GAAAACATGG GCTCGCACGA TATCGTCGAT 
GGAAACCACC GGCTGACGCT GGGCCTCATC TGGACCATCA TCCTGCGTTT CCAGATCCAA 
GATATCAGTG TGGAAACAGA AGATAATAAG GAGAAGAAAT CAGCCAAGGA CGCACTGCTG 
CTCTGGTGCC AGATGAAGAC GGCAGGTTAC CCCAATGTCA ACATCCATAA CTTCACCACA 
AGCTGGAGAG ACGGAATGGC TTTCAACGCA CTAATACACA AACACCGGCC TGACCTGATT 
GATTTTGATA AGCTGAAGAA GTCAAATGCA CACTACAATC TGCAGAACGC TTTTAATTTG 
GCTGAGCAGC ACCTTGGACT TACAAAGCTA TTGGACCCAG AGGATATCAG CGTTGACCAT 
CCTGACGAGA AGTCCATCAT CACCTACGTG GTGACATATT ACCACTACTT TTCCAAGATG 
AAGGCTCTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TGGACAACGC TATCGAAACA 
GAGAAGATGA TCGAAAAATA CGAATCACTC GCCTCTGACC TCCTGGAATG GATCGAACAG 
ACCATCATTA TCTTGAACAA TCGCAAATTT GCCAACTCAC TGGTGGGAGT CCAGCAGCAG 
CTCCAGGCGT TCAACACATA CCGCACAGTG GAGAAGCCGC CCAAATTTAC AGAAAAAGGG 
AACCTGGAAG TTCTGCTGTT CACCATCCAG AGCAAAATGA GGGCCAACAA TCAGAAAGTG 
TACATGCCCC GGGAGGGGAA GCTCATCTCC GACATCAACA AGGCCTGGGA ACGACTGGAA 
AAAGCTGAGC ACGAACGAGA ACTGGCACTG CGCAATGAGC TCATCCGACA GGAGAAACTG 
GAACAACTGG CCCGCCGATT TGATCGAAAG GCAGCTATGA GGGAGACGTG GCTGAGTGAA 
AACCAGCGTC TCGTGTCTCA GGACAACTTC GGGTTTGACC TCCCCGCCGT GGAGGCCGCC 
ACGAAAAAGC ACGAGGCCAT CGAGACGGAC ATCGCCGCCT ACGAGGAGCG AGTCCAGGCC 
GTGGTGGCCG TGGCCAAGGA GCTGGAGGCA GAGAATTATC ACGACATCAA GCGAATCACA 
GCGAGGAAGG ACAACGTGAT CCGCCTGTGG GAGTACCTGC TGGAGCTGCT CCGAGCCCGT 
AGGCAGCGCC TGGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAGAT GAAGGTGCTT TTGCTGTCTC AAGACTATGG CAAACATTTG 
CTGGGGGTAG AAGACCTGTT GCAGAAACAC GCACTGGTGG AGGCGGACAT TGGTATCCAA 
GCAGAAAGGG TGAGAGGTGT CAACGCCTCG GCTCAGAAGT TTGCCACAGA TGGGGAAGGT 
TACAAGCCCT GCGACCCCCA GGTGATCCGC GACCGCGTGG CGCACATGGA CTTCTGCTAC 
CAGGAGCTGT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAGGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCGGAAGAG GAGGGCTGGA TCAGGGAGAA GGAGCAGATC 
CTGTCCTCGG ACGACTATGG CAAAGACCTG ACCAGCGTCG TGCGCCTGCT CAGCAAGCAC 
AGGGCCTTCG AGGACGAGAT GAGCGGGCGC AGCGGCCACT TCGAGCAGGC CATCAAGGAA 
GGGGAAGACA TGATCGCCGA GGAGCACTTC GGGGCGGAGA AGATCCGGGA GCGGATCGGC 
TACATCCGGG AACAGTGGGC CAACCTGGAG CAGCTGTCGG CCATCCGGAA GAAGCGCCTG 
GAGGAGGCCT CTCTGCTCCA CCAGTTCCAG GCGGACGCGG ACGACATCGA CGCCTGGATG 
CTGGACATCC TCAAGATCGT GTCCAGCAAC GACGTGGGCC ACGACGAGTA CTCGACCCAG 
TCCCTGGTCA AGAAGCACAA GGACGTGGCC GAGGAGATTG CCAACTACCG GCCGGCCATC 
GACTCGCTGC ACGAGCAGGC CGGGGCCCTG CCCCGGGAGC ACGCCGAGTC TCCCGACGTG 
CGCGGCCGGC TGTCCGGCAT CGAGGAGCGC TACAAAGAGG TGGCGGAGCT CACCCGGCTC 
CGGAAGCAGG CCCTGCAGGA CACTCTGGCC CTCTACAAGA TGTTCAGCGA GGCCGACGCC 
TGCGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA GCATGCAGAT CCCGGAGAAG 
CTGGAGGACC TCGAGGTGAT CCAGCACAGG TTCGAGAGCT TAGAGCCGGA AATGAACAAC 
CAGGCGTCCC GTGTGGCCGT GGTGAACCAG ATCGCCCGAC AGCTGATGCA CAGCGGCCAT 
CCGAGCGAGA AGGAGATCAA GGCCCAGCAG GATAAGCTCA ACACAAGGTG GAGCCAGTTC 
CGAGAACTGG TAGACCGGAA GAAAGACGCC CTCCTCTCGG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAACGAAAC CAAGTCCTGG ATTCGGGAAA AGACCAAAGT CATCGAATCC 
ACCCAGGACC TGGGCAACGA CCTGGCGGGG GTCATGGCCC TGCAGAGGAA GCTGACCGGC 
ATGGAACGGG ATCTGGTGGC CATCGAGGCC AAGCTGACGG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CGGAGCACCC GGACCAGGCC CAGGCCATCC TGTCCCGCCT GGCGGAGATC 
AACGACGTGT GGGAGGAAAT GAAGACCACC CTGAAGAACC GCGAGGCGTC GCTGGGGGAG 
GCGAGCAAGC TCCAGCAGTT CCTGCGCGAC CTGGACGACT TCCAGTCCTG GCTGTCCCGC 
ACCCAGACGG CCATCGCCTC CGAGGACATG CCCAACACCA TGGCCGAGGC CGAGAAGCTG 
CTCACCCAGC ACGAGAACAT CAAGAATGAA ATCAACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACGCAG GGCCAGACGG ATGCCCAGTA CATGTTCCTG 
AGGCAGCGCC TGCAGGCTCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGGCAGAATC TCCTCTCCCA GTCTCACGCC TACCAGCTGT TCCTCAGGGA CACCAAGCAA 
GCGGAGGCAT TTCTCAATAA CCAGGAATAT GTCTTGGCCC ATACCGAGAT GCCCACCACC 
TTGGAGGCAG CAGAAGCTGC TATTAAGAAG CAAGAGGACT TCATGACCAC GATGGACGCC 
AACGAGGAAA AGATCAACGC GGTGGTGGAA ACCGGTCGAA GACTGGTGAG CGATGGCAAC 
ATTAACTCCG ACCGGATCCA AGAGAAAGTG GACTCCATCG ACGACAGACA TCGGAAGAAC 
CGGGAGGCAG CCAGCGAGCT TCTGATGCGA TTAAAAGATA ACAGGGATCT TCAGAAATTT 
CTGCAGGATT GCCAGGAGCT GTCCCTCTGG ATCAACGAGA AGATGCTTAC AGCCCAGGAC 
ATGTCCTACG ACGAAGCCAG AAACCTGCAC AGTAAATGGC TGAAGCACCA AGCGTTCATG 
GCAGAACTGG CTTCCAACAA AGAGTGGCTC GACAAAATCG AACAGGAAGG AATGCAGCTG 
ATTGCCGAGA AGCCCGAGAC AGAAGCAGTG GTGAAGGAGA AGCTGACGGG CTTACATAAG 
ATGTGGGAAG TTCTGGAATC CACCACCCAG ACGAAGGCCC AGCGGCTGTT CGATGCAAAT 
AAAGCCGAAC TCTTCACGCA GAGCTGTGCC GATCTGGACA AGTGGCTGAA TGGCCTGGAG 
AGCCAGATTC AGTCTGACGA CTACGGCAAA GACCTCACCA GCGTAAACAT CTTACTGAAA 
AAGCAGCAGA TGCTGGAGAA TCAGATGGAT GTGCGGAAGA AGGAGATTGA GGAACTGCAG 
AGCCAAGCCC AGGCCTTGAG CCAAGAAGGA AAAAGCACTG ACGAAGTGGA CAGCAAACGC 
CTCACCGTGC AGACCAAATT CATGGAGCTC CTGGAGCCTC TGACCGAACG GAAGAGCAAC 
CTGTTGGCTT CCAAAGAGAT TCATCAGTTC AACCGGGATG TGGAGGACGA GATTCTGTGG 
GTCGGTGAAA GGATGCCCAT AGCCACCTCC ACGGACCATG GCCACAACCT CCAGACCGTG 
CAGCTGTTGA TTAAGAAGAA CCAGACCCTC CAGAAAGAAA TCCAGGGCCA CCAGCCCCGC 
ATCGACGACA TCTACGAGAG GAGTCAGAAC ATCCTCACCG ACAGCAGCCT GAGCGCCGAA 
GCCATCAGGC AGAGGCTGGC GGACCTGAAG CAGCTGTGGA ACCTGGTCAT AGAGGAGACG 
GAGAAGCGGC ACCGGCGGCT GGAGGAATCC CACAAAGCTC AGCAGTACTA CTTCGACGCG 
GCCGAGGCCG AGGCCTGGAT GAGCGAGCAG GAGCTCTACA TGATGTCAGA AGAGAAAGCC 
AAGGATGAGC AGAGCGCCGT GTCTATGCTG AAGAAGCACC AGATTCTGGA ACAAGCGGTG 
GAGGACTACG CCGAGACCGT GCACCAGCTG TCCAAGACCA GCAGGGCTCT GGTGGCCGAC 
AATCACCCGG AGAGTGAGAG AATCAGCATG AGGCAGTCCA AAGTGGATAA ACTGTACGCA 
GGCCTGAAGG ATTTGGCCGA GGAGAGGAGA GGCAAACTGG ACGAGAGACA CAGGCTATTC 
CAGCTGAACC GGGAGGTGGA CGATCTGGAG CAGTGGATCG CCGAGAGGGA GGTGGTGGCT 
GGCTCCCATG AGCTGGGACA GGACTACGAG CACGTCACAA TGCTCCAAGA ACGATTCCGT 
GAATTTGCCC GCGACACCGG AAACATCGGG CAGGAGCGGG TGGACACGGT CAATCACATG 
GCCGACGACC TCATCAACTC CGGACATTCC GACGCCGCCA CCATCGCCGA GTGGAAGGAC 
GGCCTGAACG AGGCCTGGGC CGACCTCCTG GAGCTGATCG ACACGAGAAC GCAGATCCTG 
GCCGCCTCGT ACGAACTGCA CAAATTTTAC CACGATGCCA AGGAGATTTT CGGGCGCATC 
CAGGACAAAC ACAAGAAGCT GCCCGAGGAG CTCGGGAGGG ATCAGAACAC GGTGGAGACG 
CTCCAGAGGA TGCATACGAC ATTTGAGCAT GACATCCAGG CTTTGGGCAC CCAGGTGCGG 
CAGCTGCAGG AAGATGCCGC CCGGCTCCAG GCCGCCTACG CTGGAGACAA GGCCGACGAC 
ATCCAGAAGC GGGAGAATGA GGTCCTGGAA GCCTGGAAAG CCCTCCTGGA CGCCTGCGAG 
GGCCGCAGGG CCCGGCTGGT GGACACCGGA GACAAGTTCC GCTTCTTCAG CTTGGTGCGC 
GACCTCATGC TCTGGATGGA GGACGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG 
GATGTGTCGT CTGTGGAATT GCTCATGAAC AACCACCAGG GTATCAAAGC GGAAATTGAC 
GCCCGGAACG ACAGCTTCAC TACCTGCATC GAGCTGGGAA AATCACTGTT GGCAAGGAAA 
CACTATGCGT CGGAGGAGAT CAAGGAGAAG CTGTTGCAGT TGACGGAAAA GAGAAAAGAG 
ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGGC TGATCCTGGA GGTGCACCAG 
TTTTCGAGGG ACGCCAGCGT GGCCGAGGCC TGGCTTCTGG GCCAGGAGCC CTACCTGTCC 
AGCCGCGAGA TCGGCCAGAG CGTGGACGAG GTGGAGAAGC TCATCAAGCG GCACGAGGCC 
TTCGAGAAAT CGGCTGCCAC GTGGGACGAA CGGTTCTCGG CGCTGGAGAG GCTGACGACG 
TTGGAATTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGAAAGAG GCAGCCTCCT 
TCTCCGGAGC CGAGTACAAA GGTGTCAGAG GAAGCCGAAT CCCAGCAGCA GTGGGATGCT 
TCGAAAGGAG ACCAAGTTTC CCAAAACGGT TTGCCTGCCG AGCAGGGATC TCCACGGCTG 
GCGGAAACTG CCGAAACGAG TGAGATGGTC AACGGAGCCA CCGAGCAGAG GACGAGTTCC 
AAAGAATCCA GTCCGGTTCC TTCCCCGACT TCGGATCGTA AAGCCAAGAC CGTCCTCCAG 
GCCCAGACGG CCGCCACCTT GCCCGCCAAG ACCCAGGAGG TCCCGGCGGC CCAGATGGAG 
GGCTTCTTGA ATCGCAAGCA CGAGTGGGAG GCCCACAATA AGAAAGCCTC CAGCAGGTCT 
TGGCACAACG TATACTGTGT CATCAACAAC CAAGAGATGG GCTTCTACAA GGATGCGAAG 
GCCGCTTCTT CTGGGATACC GTACCACAAC GAGATTCCAG TTAGTTTGAA AGAAGCAGTG 
TGTGAAGTAG CCCTTGACTA CAAAAAGAAA AAGCACGTGT TTAAACTAAG ACTAAATGAC 
GGCAATGAAT ACCTTTTCCA AGCCAAAGAC GATGAGGAAA TGTCCACGTG GATCCAGGCC 
ATCACGTCCG CCATCTCCTC AGACAAGATG GACGTGTCCG CCAGCACCCA GAGCACTCCA 
GCCTCCAGCC GGGCCCAGAC GCTGCCCGCC AGCGTCACAA TAACCAGCGA GTCCAGTCCC 
GGCAAGCGGG AGAAGGACAA GGAAAAGGAC AAAGAGAAAA GGTTCAGCCT CTTTGGCAAA 
AAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003431326.2
CDS	177..7265
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYADLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVAKELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMDFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEQILSSDDYGKDLTSVVRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGAEKIRERIGYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEIANYRPAIDSLHEQAGALPREHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNSMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLTDLQKEAEKLESEHPDQAQAILSRLAEINDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTMAEAEKLLTQHENIKNEINNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQLFLRDTKQAEAFLNNQEYVLAHTEMPTTLEAAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEQEGMQLIAEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLNGLESQIQSDDYGKDLTSVNILLKKQQMLENQMDVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLTERKSNLLASKEIHQFNRDVEDEILWVGERMPIATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIYERSQNILTDSSLSAEAIRQRLADLKQLWNLVIEETEKRHRRLEESHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADNHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADDLINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKALLDACEGRRARLVDTGDKFRFFSLVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRQPPSPEPSTKVSEEAESQQQWDASKGDQVSQNGLPAEQGSPRLAETAETSEMVNGATEQRTSSKESSPVPSPTSDRKAKTVLQAQTAATLPAKTQEVPAAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKAASSGIPYHNEIPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMSTWIQAITSAISSDKMDVSASTQSTPASSRAQTLPASVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_003431278.4
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003431278.4

ORF Insert Sequence:

ATGACGACGA CGGTGGCCAC AGATTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAC 
GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAAA ACAGCTCCGC CCGGCTCTTT 
GAGCGATCCC GAATCAAGGC GCTAGCAGAT GAGCGGGAAG CAGTGCAGAA GAAGACGTTC 
ACCAAATGGG TCAACTCACA CCTGGCTCGG GTGTCTTGCC GAATCACCGA CTTGTACGCC 
GACCTGCGCG ATGGACGGAT GCTCATCAAA TTGCTCGAGG TTCTGTCCGG AGAGAGACTC 
CCCAAACCAA CCAAAGGAAG AATGCGTATC CACTGCCTGG AGAATGTGGA CAAAGCCCTC 
CAGTTCTTGA AGGAGCAGCG AGTGCACCTG GAAAACATGG GCTCGCACGA TATCGTCGAT 
GGAAACCACC GGCTGACGCT GGGCCTCATC TGGACCATCA TCCTGCGTTT CCAGATCCAA 
GATATCAGTG TGGAAACAGA AGATAATAAG GAGAAGAAAT CAGCCAAGGA CGCACTGCTG 
CTCTGGTGCC AGATGAAGAC GGCAGGTTAC CCCAATGTCA ACATCCATAA CTTCACCACA 
AGCTGGAGAG ACGGAATGGC TTTCAACGCA CTAATACACA AACACCGGCC TGACCTGATT 
GATTTTGATA AGCTGAAGAA GTCAAATGCA CACTACAATC TGCAGAACGC TTTTAATTTG 
GCTGAGCAGC ACCTTGGACT TACAAAGCTA TTGGACCCAG AGGATATCAG CGTTGACCAT 
CCTGACGAGA AGTCCATCAT CACCTACGTG GTGACATATT ACCACTACTT TTCCAAGATG 
AAGGCTCTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TGGACAACGC TATCGAAACA 
GAGAAGATGA TCGAAAAATA CGAATCACTC GCCTCTGACC TCCTGGAATG GATCGAACAG 
ACCATCATTA TCTTGAACAA TCGCAAATTT GCCAACTCAC TGGTGGGAGT CCAGCAGCAG 
CTCCAGGCGT TCAACACATA CCGCACAGTG GAGAAGCCGC CCAAATTTAC AGAAAAAGGG 
AACCTGGAAG TTCTGCTGTT CACCATCCAG AGCAAAATGA GGGCCAACAA TCAGAAAGTG 
TACATGCCCC GGGAGGGGAA GCTCATCTCC GACATCAACA AGGCCTGGGA ACGACTGGAA 
AAAGCTGAGC ACGAACGAGA ACTGGCACTG CGCAATGAGC TCATCCGACA GGAGAAACTG 
GAACAACTGG CCCGCCGATT TGATCGAAAG GCAGCTATGA GGGAGACGTG GCTGAGTGAA 
AACCAGCGTC TCGTGTCTCA GGACAACTTC GGGTTTGACC TCCCCGCCGT GGAGGCCGCC 
ACGAAAAAGC ACGAGGCCAT CGAGACGGAC ATCGCCGCCT ACGAGGAGCG AGTCCAGGCC 
GTGGTGGCCG TGGCCAAGGA GCTGGAGGCA GAGAATTATC ACGACATCAA GCGAATCACA 
GCGAGGAAGG ACAACGTGAT CCGCCTGTGG GAGTACCTGC TGGAGCTGCT CCGAGCCCGT 
AGGCAGCGCC TGGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAGAT GAAGGTGCTT TTGCTGTCTC AAGACTATGG CAAACATTTG 
CTGGGGGTAG AAGACCTGTT GCAGAAACAC GCACTGGTGG AGGCGGACAT TGGTATCCAA 
GCAGAAAGGG TGAGAGGTGT CAACGCCTCG GCTCAGAAGT TTGCCACAGA TGGGGAAGGT 
TACAAGCCCT GCGACCCCCA GGTGATCCGC GACCGCGTGG CGCACATGGA CTTCTGCTAC 
CAGGAGCTGT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAGGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCGGAAGAG GAGGGCTGGA TCAGGGAGAA GGAGCAGATC 
CTGTCCTCGG ACGACTATGG CAAAGACCTG ACCAGCGTCG TGCGCCTGCT CAGCAAGCAC 
AGGGCCTTCG AGGACGAGAT GAGCGGGCGC AGCGGCCACT TCGAGCAGGC CATCAAGGAA 
GGGGAAGACA TGATCGCCGA GGAGCACTTC GGGGCGGAGA AGATCCGGGA GCGGATCGGC 
TACATCCGGG AACAGTGGGC CAACCTGGAG CAGCTGTCGG CCATCCGGAA GAAGCGCCTG 
GAGGAGGCCT CTCTGCTCCA CCAGTTCCAG GCGGACGCGG ACGACATCGA CGCCTGGATG 
CTGGACATCC TCAAGATCGT GTCCAGCAAC GACGTGGGCC ACGACGAGTA CTCGACCCAG 
TCCCTGGTCA AGAAGCACAA GGACGTGGCC GAGGAGATTG CCAACTACCG GCCGGCCATC 
GACTCGCTGC ACGAGCAGGC CGGGGCCCTG CCCCGGGAGC ACGCCGAGTC TCCCGACGTG 
CGCGGCCGGC TGTCCGGCAT CGAGGAGCGC TACAAAGAGG TGGCGGAGCT CACCCGGCTC 
CGGAAGCAGG CCCTGCAGGA CACTCTGGCC CTCTACAAGA TGTTCAGCGA GGCCGACGCC 
TGCGAGCTCT GGATCGACGA GAAGGAGCAG TGGCTCAACA GCATGCAGAT CCCGGAGAAG 
CTGGAGGACC TCGAGGTGAT CCAGCACAGG TTCGAGAGCT TAGAGCCGGA AATGAACAAC 
CAGGCGTCCC GTGTGGCCGT GGTGAACCAG ATCGCCCGAC AGCTGATGCA CAGCGGCCAT 
CCGAGCGAGA AGGAGATCAA GGCCCAGCAG GATAAGCTCA ACACAAGGTG GAGCCAGTTC 
CGAGAACTGG TAGACCGGAA GAAAGACGCC CTCCTCTCGG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAACGAAAC CAAGTCCTGG ATTCGGGAAA AGACCAAAGT CATCGAATCC 
ACCCAGGACC TGGGCAACGA CCTGGCGGGG GTCATGGCCC TGCAGAGGAA GCTGACCGGC 
ATGGAACGGG ATCTGGTGGC CATCGAGGCC AAGCTGACGG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CGGAGCACCC GGACCAGGCC CAGGCCATCC TGTCCCGCCT GGCGGAGATC 
AACGACGTGT GGGAGGAAAT GAAGACCACC CTGAAGAACC GCGAGGCGTC GCTGGGGGAG 
GCGAGCAAGC TCCAGCAGTT CCTGCGCGAC CTGGACGACT TCCAGTCCTG GCTGTCCCGC 
ACCCAGACGG CCATCGCCTC CGAGGACATG CCCAACACCA TGGCCGAGGC CGAGAAGCTG 
CTCACCCAGC ACGAGAACAT CAAGAATGAA ATCAACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACGCAG GGCCAGACGG ATGCCCAGTA CATGTTCCTG 
AGGCAGCGCC TGCAGGCTCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGGCAGAATC TCCTCTCCCA GTCTCACGCC TACCAGCTGT TCCTCAGGGA CACCAAGCAA 
GCGGAGGCAT TTCTCAATAA CCAGGAATAT GTCTTGGCCC ATACCGAGAT GCCCACCACC 
TTGGAGGCAG CAGAAGCTGC TATTAAGAAG CAAGAGGACT TCATGACCAC GATGGACGCC 
AACGAGGAAA AGATCAACGC GGTGGTGGAA ACCGGTCGAA GACTGGTGAG CGATGGCAAC 
ATTAACTCCG ACCGGATCCA AGAGAAAGTG GACTCCATCG ACGACAGACA TCGGAAGAAC 
CGGGAGGCAG CCAGCGAGCT TCTGATGCGA TTAAAAGATA ACAGGGATCT TCAGAAATTT 
CTGCAGGATT GCCAGGAGCT GTCCCTCTGG ATCAACGAGA AGATGCTTAC AGCCCAGGAC 
ATGTCCTACG ACGAAGCCAG AAACCTGCAC AGTAAATGGC TGAAGCACCA AGCGTTCATG 
GCAGAACTGG CTTCCAACAA AGAGTGGCTC GACAAAATCG AACAGGAAGG AATGCAGCTG 
ATTGCCGAGA AGCCCGAGAC AGAAGCAGTG GTGAAGGAGA AGCTGACGGG CTTACATAAG 
ATGTGGGAAG TTCTGGAATC CACCACCCAG ACGAAGGCCC AGCGGCTGTT CGATGCAAAT 
AAAGCCGAAC TCTTCACGCA GAGCTGTGCC GATCTGGACA AGTGGCTGAA TGGCCTGGAG 
AGCCAGATTC AGTCTGACGA CTACGGCAAA GACCTCACCA GCGTAAACAT CTTACTGAAA 
AAGCAGCAGA TGCTGGAGAA TCAGATGGAT GTGCGGAAGA AGGAGATTGA GGAACTGCAG 
AGCCAAGCCC AGGCCTTGAG CCAAGAAGGA AAAAGCACTG ACGAAGTGGA CAGCAAACGC 
CTCACCGTGC AGACCAAATT CATGGAGCTC CTGGAGCCTC TGACCGAACG GAAGAGCAAC 
CTGTTGGCTT CCAAAGAGAT TCATCAGTTC AACCGGGATG TGGAGGACGA GATTCTGTGG 
GTCGGTGAAA GGATGCCCAT AGCCACCTCC ACGGACCATG GCCACAACCT CCAGACCGTG 
CAGCTGTTGA TTAAGAAGAA CCAGACCCTC CAGAAAGAAA TCCAGGGCCA CCAGCCCCGC 
ATCGACGACA TCTACGAGAG GAGTCAGAAC ATCCTCACCG ACAGCAGCCT GAGCGCCGAA 
GCCATCAGGC AGAGGCTGGC GGACCTGAAG CAGCTGTGGA ACCTGGTCAT AGAGGAGACG 
GAGAAGCGGC ACCGGCGGCT GGAGGAATCC CACAAAGCTC AGCAGTACTA CTTCGACGCG 
GCCGAGGCCG AGGCCTGGAT GAGCGAGCAG GAGCTCTACA TGATGTCAGA AGAGAAAGCC 
AAGGATGAGC AGAGCGCCGT GTCTATGCTG AAGAAGCACC AGATTCTGGA ACAAGCGGTG 
GAGGACTACG CCGAGACCGT GCACCAGCTG TCCAAGACCA GCAGGGCTCT GGTGGCCGAC 
AATCACCCGG AGAGTGAGAG AATCAGCATG AGGCAGTCCA AAGTGGATAA ACTGTACGCA 
GGCCTGAAGG ATTTGGCCGA GGAGAGGAGA GGCAAACTGG ACGAGAGACA CAGGCTATTC 
CAGCTGAACC GGGAGGTGGA CGATCTGGAG CAGTGGATCG CCGAGAGGGA GGTGGTGGCT 
GGCTCCCATG AGCTGGGACA GGACTACGAG CACGTCACAA TGCTCCAAGA ACGATTCCGT 
GAATTTGCCC GCGACACCGG AAACATCGGG CAGGAGCGGG TGGACACGGT CAATCACATG 
GCCGACGACC TCATCAACTC CGGACATTCC GACGCCGCCA CCATCGCCGA GTGGAAGGAC 
GGCCTGAACG AGGCCTGGGC CGACCTCCTG GAGCTGATCG ACACGAGAAC GCAGATCCTG 
GCCGCCTCGT ACGAACTGCA CAAATTTTAC CACGATGCCA AGGAGATTTT CGGGCGCATC 
CAGGACAAAC ACAAGAAGCT GCCCGAGGAG CTCGGGAGGG ATCAGAACAC GGTGGAGACG 
CTCCAGAGGA TGCATACGAC ATTTGAGCAT GACATCCAGG CTTTGGGCAC CCAGGTGCGG 
CAGCTGCAGG AAGATGCCGC CCGGCTCCAG GCCGCCTACG CTGGAGACAA GGCCGACGAC 
ATCCAGAAGC GGGAGAATGA GGTCCTGGAA GCCTGGAAAG CCCTCCTGGA CGCCTGCGAG 
GGCCGCAGGG CCCGGCTGGT GGACACCGGA GACAAGTTCC GCTTCTTCAG CTTGGTGCGC 
GACCTCATGC TCTGGATGGA GGACGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG 
GATGTGTCGT CTGTGGAATT GCTCATGAAC AACCACCAGG GTATCAAAGC GGAAATTGAC 
GCCCGGAACG ACAGCTTCAC TACCTGCATC GAGCTGGGAA AATCACTGTT GGCAAGGAAA 
CACTATGCGT CGGAGGAGAT CAAGGAGAAG CTGTTGCAGT TGACGGAAAA GAGAAAAGAG 
ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGGC TGATCCTGGA GGTGCACCAG 
TTTTCGAGGG ACGCCAGCGT GGCCGAGGCC TGGCTTCTGG GCCAGGAGCC CTACCTGTCC 
AGCCGCGAGA TCGGCCAGAG CGTGGACGAG GTGGAGAAGC TCATCAAGCG GCACGAGGCC 
TTCGAGAAAT CGGCTGCCAC GTGGGACGAA CGGTTCTCGG CGCTGGAGAG GCTGACGACG 
TTGGAATTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGAAAGAG GCAGCCTCCT 
TCTCCGGAGC CGAGTACAAA GGTGTCAGAG GAAGCCGAAT CCCAGCAGCA GTGGGATGCT 
TCGAAAGGAG ACCAAGTTTC CCAAAACGGT TTGCCTGCCG AGCAGGGATC TCCACGGCTG 
GCGGAAACTG CCGAAACGAG TGAGATGGTC AACGGAGCCA CCGAGCAGAG GACGAGTTCC 
AAAGAATCCA GTCCGGTTCC TTCCCCGACT TCGGATCGTA AAGCCAAGAC CGTCCTCCAG 
GCCCAGACGG CCGCCACCTT GCCCGCCAAG ACCCAGGAGG TCCCGGCGGC CCAGATGGAG 
GGCTTCTTGA ATCGCAAGCA CGAGTGGGAG GCCCACAATA AGAAAGCCTC CAGCAGGTCT 
TGGCACAACG TATACTGTGT CATCAACAAC CAAGAGATGG GCTTCTACAA GGATGCGAAG 
GCCGCTTCTT CTGGGATACC GTACCACAAC GAGATTCCAG TTAGTTTGAA AGAAGCAGTG 
TGTGAAGTAG CCCTTGACTA CAAAAAGAAA AAGCACGTGT TTAAACTAAG ACTAAATGAC 
GGCAATGAAT ACCTTTTCCA AGCCAAAGAC GATGAGGAAA TGTCCACGTG GATCCAGGCC 
ATCACGTCCG CCATCTCCTC AGACAAGATG GACGTGTCCG CCAGCACCCA GAGCACTCCA 
GCCTCCAGCC GGGCCCAGAC GCTGCCCGCC AGCGTCACAA TAACCAGCGA GTCCAGTCCC 
GGCAAGCGGG AGAAGGACAA GGAAAAGGAC AAAGAGAAAA GGTTCAGCCT CTTTGGCAAA 
AAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_029071831.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028927664.1 Ornithorhynchus anatinus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

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CloneID	OOc134131
Clone ID Related Accession (Same CDS sequence)	XM_029071831.1
Accession Version	XM_029071831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7050bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	spectrin beta chain, non-erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041736.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAC AGCGGACGGC CGGCACGGCC GCACCCCCGT CCCCCGCCTA CACCGGGCAG 
GTGCCTTACA ATTACAACCA GCTGGAGGGG AGGTTCAAGC AGCTGCAAGA TGAGCGGGAA 
GCAGTGCAGA AGAAGACGTT CACCAAATGG GTCAACTCAC ACCTGGCTCG GGTGTCTTGC 
CGAATCACCG ACTTGTACGC CGACCTGCGC GATGGACGGA TGCTCATCAA ATTGCTCGAG 
GTTCTGTCCG GAGAGAGACT CCCCAAACCA ACCAAAGGAA GAATGCGTAT CCACTGCCTG 
GAGAATGTGG ACAAAGCCCT CCAGTTCTTG AAGGAGCAGC GAGTGCACCT GGAAAACATG 
GGCTCGCACG ATATCGTCGA TGGAAACCAC CGGCTGACGC TGGGCCTCAT CTGGACCATC 
ATCCTGCGTT TCCAGATCCA AGATATCAGT GTGGAAACAG AAGATAATAA GGAGAAGAAA 
TCAGCCAAGG ACGCACTGCT GCTCTGGTGC CAGATGAAGA CGGCAGGTTA CCCCAATGTC 
AACATCCATA ACTTCACCAC AAGCTGGAGA GACGGAATGG CTTTCAACGC ACTAATACAC 
AAACACCGGC CTGACCTGAT TGATTTTGAT AAGCTGAAGA AGTCAAATGC ACACTACAAT 
CTGCAGAACG CTTTTAATTT GGCTGAGCAG CACCTTGGAC TTACAAAGCT ATTGGACCCA 
GAGGATATCA GCGTTGACCA TCCTGACGAG AAGTCCATCA TCACCTACGT GGTGACATAT 
TACCACTACT TTTCCAAGAT GAAGGCTCTG GCTGTTGAAG GAAAGCGAAT TGGAAAGGTG 
CTGGACAACG CTATCGAAAC AGAGAAGATG ATCGAAAAAT ACGAATCACT CGCCTCTGAC 
CTCCTGGAAT GGATCGAACA GACCATCATT ATCTTGAACA ATCGCAAATT TGCCAACTCA 
CTGGTGGGAG TCCAGCAGCA GCTCCAGGCG TTCAACACAT ACCGCACAGT GGAGAAGCCG 
CCCAAATTTA CAGAAAAAGG GAACCTGGAA GTTCTGCTGT TCACCATCCA GAGCAAAATG 
AGGGCCAACA ATCAGAAAGT GTACATGCCC CGGGAGGGGA AGCTCATCTC CGACATCAAC 
AAGGCCTGGG AACGACTGGA AAAAGCTGAG CACGAACGAG AACTGGCACT GCGCAATGAG 
CTCATCCGAC AGGAGAAACT GGAACAACTG GCCCGCCGAT TTGATCGAAA GGCAGCTATG 
AGGGAGACGT GGCTGAGTGA AAACCAGCGT CTCGTGTCTC AGGACAACTT CGGGTTTGAC 
CTCCCCGCCG TGGAGGCCGC CACGAAAAAG CACGAGGCCA TCGAGACGGA CATCGCCGCC 
TACGAGGAGC GAGTCCAGGC CGTGGTGGCC GTGGCCAAGG AGCTGGAGGC AGAGAATTAT 
CACGACATCA AGCGAATCAC AGCGAGGAAG GACAACGTGA TCCGCCTGTG GGAGTACCTG 
CTGGAGCTGC TCCGAGCCCG TAGGCAGCGC CTGGAGATGA ACCTGGGGCT GCAGAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAGA TGAAGGTGCT TTTGCTGTCT 
CAAGACTATG GCAAACATTT GCTGGGGGTA GAAGACCTGT TGCAGAAACA CGCACTGGTG 
GAGGCGGACA TTGGTATCCA AGCAGAAAGG GTGAGAGGTG TCAACGCCTC GGCTCAGAAG 
TTTGCCACAG ATGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTGATCCG CGACCGCGTG 
GCGCACATGG ACTTCTGCTA CCAGGAGCTG TGCCAGCTGG CGGCCGAGCG CCGGGCCCGC 
CTGGAGGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCGGAAGA GGAGGGCTGG 
ATCAGGGAGA AGGAGCAGAT CCTGTCCTCG GACGACTATG GCAAAGACCT GACCAGCGTC 
GTGCGCCTGC TCAGCAAGCA CAGGGCCTTC GAGGACGAGA TGAGCGGGCG CAGCGGCCAC 
TTCGAGCAGG CCATCAAGGA AGGGGAAGAC ATGATCGCCG AGGAGCACTT CGGGGCGGAG 
AAGATCCGGG AGCGGATCGG CTACATCCGG GAACAGTGGG CCAACCTGGA GCAGCTGTCG 
GCCATCCGGA AGAAGCGCCT GGAGGAGGCC TCTCTGCTCC ACCAGTTCCA GGCGGACGCG 
GACGACATCG ACGCCTGGAT GCTGGACATC CTCAAGATCG TGTCCAGCAA CGACGTGGGC 
CACGACGAGT ACTCGACCCA GTCCCTGGTC AAGAAGCACA AGGACGTGGC CGAGGAGATT 
GCCAACTACC GGCCGGCCAT CGACTCGCTG CACGAGCAGG CCGGGGCCCT GCCCCGGGAG 
CACGCCGAGT CTCCCGACGT GCGCGGCCGG CTGTCCGGCA TCGAGGAGCG CTACAAAGAG 
GTGGCGGAGC TCACCCGGCT CCGGAAGCAG GCCCTGCAGG ACACTCTGGC CCTCTACAAG 
ATGTTCAGCG AGGCCGACGC CTGCGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AGCATGCAGA TCCCGGAGAA GCTGGAGGAC CTCGAGGTGA TCCAGCACAG GTTCGAGAGC 
TTAGAGCCGG AAATGAACAA CCAGGCGTCC CGTGTGGCCG TGGTGAACCA GATCGCCCGA 
CAGCTGATGC ACAGCGGCCA TCCGAGCGAG AAGGAGATCA AGGCCCAGCA GGATAAGCTC 
AACACAAGGT GGAGCCAGTT CCGAGAACTG GTAGACCGGA AGAAAGACGC CCTCCTCTCG 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAACGAAA CCAAGTCCTG GATTCGGGAA 
AAGACCAAAG TCATCGAATC CACCCAGGAC CTGGGCAACG ACCTGGCGGG GGTCATGGCC 
CTGCAGAGGA AGCTGACCGG CATGGAACGG GATCTGGTGG CCATCGAGGC CAAGCTGACG 
GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCGGAGCACC CGGACCAGGC CCAGGCCATC 
CTGTCCCGCC TGGCGGAGAT CAACGACGTG TGGGAGGAAA TGAAGACCAC CCTGAAGAAC 
CGCGAGGCGT CGCTGGGGGA GGCGAGCAAG CTCCAGCAGT TCCTGCGCGA CCTGGACGAC 
TTCCAGTCCT GGCTGTCCCG CACCCAGACG GCCATCGCCT CCGAGGACAT GCCCAACACC 
ATGGCCGAGG CCGAGAAGCT GCTCACCCAG CACGAGAACA TCAAGAATGA AATCAACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACGCA GGGCCAGACG 
GATGCCCAGT ACATGTTCCT GAGGCAGCGC CTGCAGGCTC TGGACACAGG CTGGAACGAG 
CTCCACAAGA TGTGGGAGAA CAGGCAGAAT CTCCTCTCCC AGTCTCACGC CTACCAGCTG 
TTCCTCAGGG ACACCAAGCA AGCGGAGGCA TTTCTCAATA ACCAGGAATA TGTCTTGGCC 
CATACCGAGA TGCCCACCAC CTTGGAGGCA GCAGAAGCTG CTATTAAGAA GCAAGAGGAC 
TTCATGACCA CGATGGACGC CAACGAGGAA AAGATCAACG CGGTGGTGGA AACCGGTCGA 
AGACTGGTGA GCGATGGCAA CATTAACTCC GACCGGATCC AAGAGAAAGT GGACTCCATC 
GACGACAGAC ATCGGAAGAA CCGGGAGGCA GCCAGCGAGC TTCTGATGCG ATTAAAAGAT 
AACAGGGATC TTCAGAAATT TCTGCAGGAT TGCCAGGAGC TGTCCCTCTG GATCAACGAG 
AAGATGCTTA CAGCCCAGGA CATGTCCTAC GACGAAGCCA GAAACCTGCA CAGTAAATGG 
CTGAAGCACC AAGCGTTCAT GGCAGAACTG GCTTCCAACA AAGAGTGGCT CGACAAAATC 
GAACAGGAAG GAATGCAGCT GATTGCCGAG AAGCCCGAGA CAGAAGCAGT GGTGAAGGAG 
AAGCTGACGG GCTTACATAA GATGTGGGAA GTTCTGGAAT CCACCACCCA GACGAAGGCC 
CAGCGGCTGT TCGATGCAAA TAAAGCCGAA CTCTTCACGC AGAGCTGTGC CGATCTGGAC 
AAGTGGCTGA ATGGCCTGGA GAGCCAGATT CAGTCTGACG ACTACGGCAA AGACCTCACC 
AGCGTAAACA TCTTACTGAA AAAGCAGCAG ATGCTGGAGA ATCAGATGGA TGTGCGGAAG 
AAGGAGATTG AGGAACTGCA GAGCCAAGCC CAGGCCTTGA GCCAAGAAGG AAAAAGCACT 
GACGAAGTGG ACAGCAAACG CCTCACCGTG CAGACCAAAT TCATGGAGCT CCTGGAGCCT 
CTGACCGAAC GGAAGAGCAA CCTGTTGGCT TCCAAAGAGA TTCATCAGTT CAACCGGGAT 
GTGGAGGACG AGATTCTGTG GGTCGGTGAA AGGATGCCCA TAGCCACCTC CACGGACCAT 
GGCCACAACC TCCAGACCGT GCAGCTGTTG ATTAAGAAGA ACCAGACCCT CCAGAAAGAA 
ATCCAGGGCC ACCAGCCCCG CATCGACGAC ATCTACGAGA GGAGTCAGAA CATCCTCACC 
GACAGCAGCC TGAGCGCCGA AGCCATCAGG CAGAGGCTGG CGGACCTGAA GCAGCTGTGG 
AACCTGGTCA TAGAGGAGAC GGAGAAGCGG CACCGGCGGC TGGAGGAATC CCACAAAGCT 
CAGCAGTACT ACTTCGACGC GGCCGAGGCC GAGGCCTGGA TGAGCGAGCA GGAGCTCTAC 
ATGATGTCAG AAGAGAAAGC CAAGGATGAG CAGAGCGCCG TGTCTATGCT GAAGAAGCAC 
CAGATTCTGG AACAAGCGGT GGAGGACTAC GCCGAGACCG TGCACCAGCT GTCCAAGACC 
AGCAGGGCTC TGGTGGCCGA CAATCACCCG GAGAGTGAGA GAATCAGCAT GAGGCAGTCC 
AAAGTGGATA AACTGTACGC AGGCCTGAAG GATTTGGCCG AGGAGAGGAG AGGCAAACTG 
GACGAGAGAC ACAGGCTATT CCAGCTGAAC CGGGAGGTGG ACGATCTGGA GCAGTGGATC 
GCCGAGAGGG AGGTGGTGGC TGGCTCCCAT GAGCTGGGAC AGGACTACGA GCACGTCACA 
ATGCTCCAAG AACGATTCCG TGAATTTGCC CGCGACACCG GAAACATCGG GCAGGAGCGG 
GTGGACACGG TCAATCACAT GGCCGACGAC CTCATCAACT CCGGACATTC CGACGCCGCC 
ACCATCGCCG AGTGGAAGGA CGGCCTGAAC GAGGCCTGGG CCGACCTCCT GGAGCTGATC 
GACACGAGAA CGCAGATCCT GGCCGCCTCG TACGAACTGC ACAAATTTTA CCACGATGCC 
AAGGAGATTT TCGGGCGCAT CCAGGACAAA CACAAGAAGC TGCCCGAGGA GCTCGGGAGG 
GATCAGAACA CGGTGGAGAC GCTCCAGAGG ATGCATACGA CATTTGAGCA TGACATCCAG 
GCTTTGGGCA CCCAGGTGCG GCAGCTGCAG GAAGATGCCG CCCGGCTCCA GGCCGCCTAC 
GCTGGAGACA AGGCCGACGA CATCCAGAAG CGGGAGAATG AGGTCCTGGA AGCCTGGAAA 
GCCCTCCTGG ACGCCTGCGA GGGCCGCAGG GCCCGGCTGG TGGACACCGG AGACAAGTTC 
CGCTTCTTCA GCTTGGTGCG CGACCTCATG CTCTGGATGG AGGACGTCAT CCGGCAGATC 
GAAGCCCAGG AGAAGCCCCG GGATGTGTCG TCTGTGGAAT TGCTCATGAA CAACCACCAG 
GGTATCAAAG CGGAAATTGA CGCCCGGAAC GACAGCTTCA CTACCTGCAT CGAGCTGGGA 
AAATCACTGT TGGCAAGGAA ACACTATGCG TCGGAGGAGA TCAAGGAGAA GCTGTTGCAG 
TTGACGGAAA AGAGAAAAGA GATGATTGAC AAGTGGGAAG ACCGATGGGA GTGGTTAAGG 
CTGATCCTGG AGGTGCACCA GTTTTCGAGG GACGCCAGCG TGGCCGAGGC CTGGCTTCTG 
GGCCAGGAGC CCTACCTGTC CAGCCGCGAG ATCGGCCAGA GCGTGGACGA GGTGGAGAAG 
CTCATCAAGC GGCACGAGGC CTTCGAGAAA TCGGCTGCCA CGTGGGACGA ACGGTTCTCG 
GCGCTGGAGA GGCTGACGAC GTTGGAATTA CTGGAAGTGC GCAGACAGCA AGAGGAAGAG 
GAGAGAAAGA GGCAGCCTCC TTCTCCGGAG CCGAGTACAA AGGTGTCAGA GGAAGCCGAA 
TCCCAGCAGC AGTGGGATGC TTCGAAAGGA GACCAAGTTT CCCAAAACGG TTTGCCTGCC 
GAGCAGGGAT CTCCACGGCT GGCGGAAACT GCCGAAACGA GTGAGATGGT CAACGGAGCC 
ACCGAGCAGA GGACGAGTTC CAAAGAATCC AGTCCGGTTC CTTCCCCGAC TTCGGATCGT 
AAAGCCAAGA CCGTCCTCCA GGCCCAGACG GCCGCCACCT TGCCCGCCAA GACCCAGGAG 
GTCCCGGCGG CCCAGATGGA GGGCTTCTTG AATCGCAAGC ACGAGTGGGA GGCCCACAAT 
AAGAAAGCCT CCAGCAGGTC TTGGCACAAC GTATACTGTG TCATCAACAA CCAAGAGATG 
GGCTTCTACA AGGATGCGAA GGCCGCTTCT TCTGGGATAC CGTACCACAA CGAGATTCCA 
GTTAGTTTGA AAGAAGCAGT GTGTGAAGTA GCCCTTGACT ACAAAAAGAA AAAGCACGTG 
TTTAAACTAA GACTAAATGA CGGCAATGAA TACCTTTTCC AAGCCAAAGA CGATGAGGAA 
ATGTCCACGT GGATCCAGGC CATCACGTCC GCCATCTCCT CAGACAAGAT GGACGTGTCC 
GCCAGCACCC AGAGCACTCC AGCCTCCAGC CGGGCCCAGA CGCTGCCCGC CAGCGTCACA 
ATAACCAGCG AGTCCAGTCC CGGCAAGCGG GAGAAGGACA AGGAAAAGGA CAAAGAGAAA 
AGGTTCAGCC TCTTTGGCAA AAAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028927664.1
CDS	19..7068
Translation	MELQRTAGTAAPPSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYADLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVAKELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMDFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEQILSSDDYGKDLTSVVRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGAEKIRERIGYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEIANYRPAIDSLHEQAGALPREHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNSMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLTDLQKEAEKLESEHPDQAQAILSRLAEINDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTMAEAEKLLTQHENIKNEINNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQLFLRDTKQAEAFLNNQEYVLAHTEMPTTLEAAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEQEGMQLIAEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLNGLESQIQSDDYGKDLTSVNILLKKQQMLENQMDVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLTERKSNLLASKEIHQFNRDVEDEILWVGERMPIATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIYERSQNILTDSSLSAEAIRQRLADLKQLWNLVIEETEKRHRRLEESHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADNHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADDLINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKALLDACEGRRARLVDTGDKFRFFSLVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRQPPSPEPSTKVSEEAESQQQWDASKGDQVSQNGLPAEQGSPRLAETAETSEMVNGATEQRTSSKESSPVPSPTSDRKAKTVLQAQTAATLPAKTQEVPAAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKAASSGIPYHNEIPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMSTWIQAITSAISSDKMDVSASTQSTPASSRAQTLPASVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_029071831.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029071831.1

ORF Insert Sequence:

ATGGAATTAC AGCGGACGGC CGGCACGGCC GCACCCCCGT CCCCCGCCTA CACCGGGCAG 
GTGCCTTACA ATTACAACCA GCTGGAGGGG AGGTTCAAGC AGCTGCAAGA TGAGCGGGAA 
GCAGTGCAGA AGAAGACGTT CACCAAATGG GTCAACTCAC ACCTGGCTCG GGTGTCTTGC 
CGAATCACCG ACTTGTACGC CGACCTGCGC GATGGACGGA TGCTCATCAA ATTGCTCGAG 
GTTCTGTCCG GAGAGAGACT CCCCAAACCA ACCAAAGGAA GAATGCGTAT CCACTGCCTG 
GAGAATGTGG ACAAAGCCCT CCAGTTCTTG AAGGAGCAGC GAGTGCACCT GGAAAACATG 
GGCTCGCACG ATATCGTCGA TGGAAACCAC CGGCTGACGC TGGGCCTCAT CTGGACCATC 
ATCCTGCGTT TCCAGATCCA AGATATCAGT GTGGAAACAG AAGATAATAA GGAGAAGAAA 
TCAGCCAAGG ACGCACTGCT GCTCTGGTGC CAGATGAAGA CGGCAGGTTA CCCCAATGTC 
AACATCCATA ACTTCACCAC AAGCTGGAGA GACGGAATGG CTTTCAACGC ACTAATACAC 
AAACACCGGC CTGACCTGAT TGATTTTGAT AAGCTGAAGA AGTCAAATGC ACACTACAAT 
CTGCAGAACG CTTTTAATTT GGCTGAGCAG CACCTTGGAC TTACAAAGCT ATTGGACCCA 
GAGGATATCA GCGTTGACCA TCCTGACGAG AAGTCCATCA TCACCTACGT GGTGACATAT 
TACCACTACT TTTCCAAGAT GAAGGCTCTG GCTGTTGAAG GAAAGCGAAT TGGAAAGGTG 
CTGGACAACG CTATCGAAAC AGAGAAGATG ATCGAAAAAT ACGAATCACT CGCCTCTGAC 
CTCCTGGAAT GGATCGAACA GACCATCATT ATCTTGAACA ATCGCAAATT TGCCAACTCA 
CTGGTGGGAG TCCAGCAGCA GCTCCAGGCG TTCAACACAT ACCGCACAGT GGAGAAGCCG 
CCCAAATTTA CAGAAAAAGG GAACCTGGAA GTTCTGCTGT TCACCATCCA GAGCAAAATG 
AGGGCCAACA ATCAGAAAGT GTACATGCCC CGGGAGGGGA AGCTCATCTC CGACATCAAC 
AAGGCCTGGG AACGACTGGA AAAAGCTGAG CACGAACGAG AACTGGCACT GCGCAATGAG 
CTCATCCGAC AGGAGAAACT GGAACAACTG GCCCGCCGAT TTGATCGAAA GGCAGCTATG 
AGGGAGACGT GGCTGAGTGA AAACCAGCGT CTCGTGTCTC AGGACAACTT CGGGTTTGAC 
CTCCCCGCCG TGGAGGCCGC CACGAAAAAG CACGAGGCCA TCGAGACGGA CATCGCCGCC 
TACGAGGAGC GAGTCCAGGC CGTGGTGGCC GTGGCCAAGG AGCTGGAGGC AGAGAATTAT 
CACGACATCA AGCGAATCAC AGCGAGGAAG GACAACGTGA TCCGCCTGTG GGAGTACCTG 
CTGGAGCTGC TCCGAGCCCG TAGGCAGCGC CTGGAGATGA ACCTGGGGCT GCAGAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAGA TGAAGGTGCT TTTGCTGTCT 
CAAGACTATG GCAAACATTT GCTGGGGGTA GAAGACCTGT TGCAGAAACA CGCACTGGTG 
GAGGCGGACA TTGGTATCCA AGCAGAAAGG GTGAGAGGTG TCAACGCCTC GGCTCAGAAG 
TTTGCCACAG ATGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTGATCCG CGACCGCGTG 
GCGCACATGG ACTTCTGCTA CCAGGAGCTG TGCCAGCTGG CGGCCGAGCG CCGGGCCCGC 
CTGGAGGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCGGAAGA GGAGGGCTGG 
ATCAGGGAGA AGGAGCAGAT CCTGTCCTCG GACGACTATG GCAAAGACCT GACCAGCGTC 
GTGCGCCTGC TCAGCAAGCA CAGGGCCTTC GAGGACGAGA TGAGCGGGCG CAGCGGCCAC 
TTCGAGCAGG CCATCAAGGA AGGGGAAGAC ATGATCGCCG AGGAGCACTT CGGGGCGGAG 
AAGATCCGGG AGCGGATCGG CTACATCCGG GAACAGTGGG CCAACCTGGA GCAGCTGTCG 
GCCATCCGGA AGAAGCGCCT GGAGGAGGCC TCTCTGCTCC ACCAGTTCCA GGCGGACGCG 
GACGACATCG ACGCCTGGAT GCTGGACATC CTCAAGATCG TGTCCAGCAA CGACGTGGGC 
CACGACGAGT ACTCGACCCA GTCCCTGGTC AAGAAGCACA AGGACGTGGC CGAGGAGATT 
GCCAACTACC GGCCGGCCAT CGACTCGCTG CACGAGCAGG CCGGGGCCCT GCCCCGGGAG 
CACGCCGAGT CTCCCGACGT GCGCGGCCGG CTGTCCGGCA TCGAGGAGCG CTACAAAGAG 
GTGGCGGAGC TCACCCGGCT CCGGAAGCAG GCCCTGCAGG ACACTCTGGC CCTCTACAAG 
ATGTTCAGCG AGGCCGACGC CTGCGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AGCATGCAGA TCCCGGAGAA GCTGGAGGAC CTCGAGGTGA TCCAGCACAG GTTCGAGAGC 
TTAGAGCCGG AAATGAACAA CCAGGCGTCC CGTGTGGCCG TGGTGAACCA GATCGCCCGA 
CAGCTGATGC ACAGCGGCCA TCCGAGCGAG AAGGAGATCA AGGCCCAGCA GGATAAGCTC 
AACACAAGGT GGAGCCAGTT CCGAGAACTG GTAGACCGGA AGAAAGACGC CCTCCTCTCG 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAACGAAA CCAAGTCCTG GATTCGGGAA 
AAGACCAAAG TCATCGAATC CACCCAGGAC CTGGGCAACG ACCTGGCGGG GGTCATGGCC 
CTGCAGAGGA AGCTGACCGG CATGGAACGG GATCTGGTGG CCATCGAGGC CAAGCTGACG 
GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCGGAGCACC CGGACCAGGC CCAGGCCATC 
CTGTCCCGCC TGGCGGAGAT CAACGACGTG TGGGAGGAAA TGAAGACCAC CCTGAAGAAC 
CGCGAGGCGT CGCTGGGGGA GGCGAGCAAG CTCCAGCAGT TCCTGCGCGA CCTGGACGAC 
TTCCAGTCCT GGCTGTCCCG CACCCAGACG GCCATCGCCT CCGAGGACAT GCCCAACACC 
ATGGCCGAGG CCGAGAAGCT GCTCACCCAG CACGAGAACA TCAAGAATGA AATCAACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACGCA GGGCCAGACG 
GATGCCCAGT ACATGTTCCT GAGGCAGCGC CTGCAGGCTC TGGACACAGG CTGGAACGAG 
CTCCACAAGA TGTGGGAGAA CAGGCAGAAT CTCCTCTCCC AGTCTCACGC CTACCAGCTG 
TTCCTCAGGG ACACCAAGCA AGCGGAGGCA TTTCTCAATA ACCAGGAATA TGTCTTGGCC 
CATACCGAGA TGCCCACCAC CTTGGAGGCA GCAGAAGCTG CTATTAAGAA GCAAGAGGAC 
TTCATGACCA CGATGGACGC CAACGAGGAA AAGATCAACG CGGTGGTGGA AACCGGTCGA 
AGACTGGTGA GCGATGGCAA CATTAACTCC GACCGGATCC AAGAGAAAGT GGACTCCATC 
GACGACAGAC ATCGGAAGAA CCGGGAGGCA GCCAGCGAGC TTCTGATGCG ATTAAAAGAT 
AACAGGGATC TTCAGAAATT TCTGCAGGAT TGCCAGGAGC TGTCCCTCTG GATCAACGAG 
AAGATGCTTA CAGCCCAGGA CATGTCCTAC GACGAAGCCA GAAACCTGCA CAGTAAATGG 
CTGAAGCACC AAGCGTTCAT GGCAGAACTG GCTTCCAACA AAGAGTGGCT CGACAAAATC 
GAACAGGAAG GAATGCAGCT GATTGCCGAG AAGCCCGAGA CAGAAGCAGT GGTGAAGGAG 
AAGCTGACGG GCTTACATAA GATGTGGGAA GTTCTGGAAT CCACCACCCA GACGAAGGCC 
CAGCGGCTGT TCGATGCAAA TAAAGCCGAA CTCTTCACGC AGAGCTGTGC CGATCTGGAC 
AAGTGGCTGA ATGGCCTGGA GAGCCAGATT CAGTCTGACG ACTACGGCAA AGACCTCACC 
AGCGTAAACA TCTTACTGAA AAAGCAGCAG ATGCTGGAGA ATCAGATGGA TGTGCGGAAG 
AAGGAGATTG AGGAACTGCA GAGCCAAGCC CAGGCCTTGA GCCAAGAAGG AAAAAGCACT 
GACGAAGTGG ACAGCAAACG CCTCACCGTG CAGACCAAAT TCATGGAGCT CCTGGAGCCT 
CTGACCGAAC GGAAGAGCAA CCTGTTGGCT TCCAAAGAGA TTCATCAGTT CAACCGGGAT 
GTGGAGGACG AGATTCTGTG GGTCGGTGAA AGGATGCCCA TAGCCACCTC CACGGACCAT 
GGCCACAACC TCCAGACCGT GCAGCTGTTG ATTAAGAAGA ACCAGACCCT CCAGAAAGAA 
ATCCAGGGCC ACCAGCCCCG CATCGACGAC ATCTACGAGA GGAGTCAGAA CATCCTCACC 
GACAGCAGCC TGAGCGCCGA AGCCATCAGG CAGAGGCTGG CGGACCTGAA GCAGCTGTGG 
AACCTGGTCA TAGAGGAGAC GGAGAAGCGG CACCGGCGGC TGGAGGAATC CCACAAAGCT 
CAGCAGTACT ACTTCGACGC GGCCGAGGCC GAGGCCTGGA TGAGCGAGCA GGAGCTCTAC 
ATGATGTCAG AAGAGAAAGC CAAGGATGAG CAGAGCGCCG TGTCTATGCT GAAGAAGCAC 
CAGATTCTGG AACAAGCGGT GGAGGACTAC GCCGAGACCG TGCACCAGCT GTCCAAGACC 
AGCAGGGCTC TGGTGGCCGA CAATCACCCG GAGAGTGAGA GAATCAGCAT GAGGCAGTCC 
AAAGTGGATA AACTGTACGC AGGCCTGAAG GATTTGGCCG AGGAGAGGAG AGGCAAACTG 
GACGAGAGAC ACAGGCTATT CCAGCTGAAC CGGGAGGTGG ACGATCTGGA GCAGTGGATC 
GCCGAGAGGG AGGTGGTGGC TGGCTCCCAT GAGCTGGGAC AGGACTACGA GCACGTCACA 
ATGCTCCAAG AACGATTCCG TGAATTTGCC CGCGACACCG GAAACATCGG GCAGGAGCGG 
GTGGACACGG TCAATCACAT GGCCGACGAC CTCATCAACT CCGGACATTC CGACGCCGCC 
ACCATCGCCG AGTGGAAGGA CGGCCTGAAC GAGGCCTGGG CCGACCTCCT GGAGCTGATC 
GACACGAGAA CGCAGATCCT GGCCGCCTCG TACGAACTGC ACAAATTTTA CCACGATGCC 
AAGGAGATTT TCGGGCGCAT CCAGGACAAA CACAAGAAGC TGCCCGAGGA GCTCGGGAGG 
GATCAGAACA CGGTGGAGAC GCTCCAGAGG ATGCATACGA CATTTGAGCA TGACATCCAG 
GCTTTGGGCA CCCAGGTGCG GCAGCTGCAG GAAGATGCCG CCCGGCTCCA GGCCGCCTAC 
GCTGGAGACA AGGCCGACGA CATCCAGAAG CGGGAGAATG AGGTCCTGGA AGCCTGGAAA 
GCCCTCCTGG ACGCCTGCGA GGGCCGCAGG GCCCGGCTGG TGGACACCGG AGACAAGTTC 
CGCTTCTTCA GCTTGGTGCG CGACCTCATG CTCTGGATGG AGGACGTCAT CCGGCAGATC 
GAAGCCCAGG AGAAGCCCCG GGATGTGTCG TCTGTGGAAT TGCTCATGAA CAACCACCAG 
GGTATCAAAG CGGAAATTGA CGCCCGGAAC GACAGCTTCA CTACCTGCAT CGAGCTGGGA 
AAATCACTGT TGGCAAGGAA ACACTATGCG TCGGAGGAGA TCAAGGAGAA GCTGTTGCAG 
TTGACGGAAA AGAGAAAAGA GATGATTGAC AAGTGGGAAG ACCGATGGGA GTGGTTAAGG 
CTGATCCTGG AGGTGCACCA GTTTTCGAGG GACGCCAGCG TGGCCGAGGC CTGGCTTCTG 
GGCCAGGAGC CCTACCTGTC CAGCCGCGAG ATCGGCCAGA GCGTGGACGA GGTGGAGAAG 
CTCATCAAGC GGCACGAGGC CTTCGAGAAA TCGGCTGCCA CGTGGGACGA ACGGTTCTCG 
GCGCTGGAGA GGCTGACGAC GTTGGAATTA CTGGAAGTGC GCAGACAGCA AGAGGAAGAG 
GAGAGAAAGA GGCAGCCTCC TTCTCCGGAG CCGAGTACAA AGGTGTCAGA GGAAGCCGAA 
TCCCAGCAGC AGTGGGATGC TTCGAAAGGA GACCAAGTTT CCCAAAACGG TTTGCCTGCC 
GAGCAGGGAT CTCCACGGCT GGCGGAAACT GCCGAAACGA GTGAGATGGT CAACGGAGCC 
ACCGAGCAGA GGACGAGTTC CAAAGAATCC AGTCCGGTTC CTTCCCCGAC TTCGGATCGT 
AAAGCCAAGA CCGTCCTCCA GGCCCAGACG GCCGCCACCT TGCCCGCCAA GACCCAGGAG 
GTCCCGGCGG CCCAGATGGA GGGCTTCTTG AATCGCAAGC ACGAGTGGGA GGCCCACAAT 
AAGAAAGCCT CCAGCAGGTC TTGGCACAAC GTATACTGTG TCATCAACAA CCAAGAGATG 
GGCTTCTACA AGGATGCGAA GGCCGCTTCT TCTGGGATAC CGTACCACAA CGAGATTCCA 
GTTAGTTTGA AAGAAGCAGT GTGTGAAGTA GCCCTTGACT ACAAAAAGAA AAAGCACGTG 
TTTAAACTAA GACTAAATGA CGGCAATGAA TACCTTTTCC AAGCCAAAGA CGATGAGGAA 
ATGTCCACGT GGATCCAGGC CATCACGTCC GCCATCTCCT CAGACAAGAT GGACGTGTCC 
GCCAGCACCC AGAGCACTCC AGCCTCCAGC CGGGCCCAGA CGCTGCCCGC CAGCGTCACA 
ATAACCAGCG AGTCCAGTCC CGGCAAGCGG GAGAAGGACA AGGAAAAGGA CAAAGAGAAA 
AGGTTCAGCC TCTTTGGCAA AAAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_029071832.1 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_028927665.1 Ornithorhynchus anatinus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

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CloneID	OOc134132
Clone ID Related Accession (Same CDS sequence)	XM_029071832.1
Accession Version	XM_029071832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1556380800000
Organism	Ornithorhynchus anatinus(platypus)
Product	spectrin beta chain, non-erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_041736.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ornithorhynchus anatinus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTAC AGCGGACGGC CGGCACGGCC GCACCCCCGT CCCCCGCCTA CACCGGGCAG 
GTGCCTTACA ATTACAACCA GCTGGAGGGG AGGTTCAAGC AGCTGCAAGA TGAGCGGGAA 
GCAGTGCAGA AGAAGACGTT CACCAAATGG GTCAACTCAC ACCTGGCTCG GGTGTCTTGC 
CGAATCACCG ACTTGTACGC CGACCTGCGC GATGGACGGA TGCTCATCAA ATTGCTCGAG 
GTTCTGTCCG GAGAGAGACT CCCCAAACCA ACCAAAGGAA GAATGCGTAT CCACTGCCTG 
GAGAATGTGG ACAAAGCCCT CCAGTTCTTG AAGGAGCAGC GAGTGCACCT GGAAAACATG 
GGCTCGCACG ATATCGTCGA TGGAAACCAC CGGCTGACGC TGGGCCTCAT CTGGACCATC 
ATCCTGCGTT TCCAGATCCA AGATATCAGT GTGGAAACAG AAGATAATAA GGAGAAGAAA 
TCAGCCAAGG ACGCACTGCT GCTCTGGTGC CAGATGAAGA CGGCAGGTTA CCCCAATGTC 
AACATCCATA ACTTCACCAC AAGCTGGAGA GACGGAATGG CTTTCAACGC ACTAATACAC 
AAACACCGGC CTGACCTGAT TGATTTTGAT AAGCTGAAGA AGTCAAATGC ACACTACAAT 
CTGCAGAACG CTTTTAATTT GGCTGAGCAG CACCTTGGAC TTACAAAGCT ATTGGACCCA 
GAGGATATCA GCGTTGACCA TCCTGACGAG AAGTCCATCA TCACCTACGT GGTGACATAT 
TACCACTACT TTTCCAAGAT GAAGGCTCTG GCTGTTGAAG GAAAGCGAAT TGGAAAGGTG 
CTGGACAACG CTATCGAAAC AGAGAAGATG ATCGAAAAAT ACGAATCACT CGCCTCTGAC 
CTCCTGGAAT GGATCGAACA GACCATCATT ATCTTGAACA ATCGCAAATT TGCCAACTCA 
CTGGTGGGAG TCCAGCAGCA GCTCCAGGCG TTCAACACAT ACCGCACAGT GGAGAAGCCG 
CCCAAATTTA CAGAAAAAGG GAACCTGGAA GTTCTGCTGT TCACCATCCA GAGCAAAATG 
AGGGCCAACA ATCAGAAAGT GTACATGCCC CGGGAGGGGA AGCTCATCTC CGACATCAAC 
AAGGCCTGGG AACGACTGGA AAAAGCTGAG CACGAACGAG AACTGGCACT GCGCAATGAG 
CTCATCCGAC AGGAGAAACT GGAACAACTG GCCCGCCGAT TTGATCGAAA GGCAGCTATG 
AGGGAGACGT GGCTGAGTGA AAACCAGCGT CTCGTGTCTC AGGACAACTT CGGGTTTGAC 
CTCCCCGCCG TGGAGGCCGC CACGAAAAAG CACGAGGCCA TCGAGACGGA CATCGCCGCC 
TACGAGGAGC GAGTCCAGGC CGTGGTGGCC GTGGCCAAGG AGCTGGAGGC AGAGAATTAT 
CACGACATCA AGCGAATCAC AGCGAGGAAG GACAACGTGA TCCGCCTGTG GGAGTACCTG 
CTGGAGCTGC TCCGAGCCCG TAGGCAGCGC CTGGAGATGA ACCTGGGGCT GCAGAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAGA TGAAGGTGCT TTTGCTGTCT 
CAAGACTATG GCAAACATTT GCTGGGGGTA GAAGACCTGT TGCAGAAACA CGCACTGGTG 
GAGGCGGACA TTGGTATCCA AGCAGAAAGG GTGAGAGGTG TCAACGCCTC GGCTCAGAAG 
TTTGCCACAG ATGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTGATCCG CGACCGCGTG 
GCGCACATGG ACTTCTGCTA CCAGGAGCTG TGCCAGCTGG CGGCCGAGCG CCGGGCCCGC 
CTGGAGGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCGGAAGA GGAGGGCTGG 
ATCAGGGAGA AGGAGCAGAT CCTGTCCTCG GACGACTATG GCAAAGACCT GACCAGCGTC 
GTGCGCCTGC TCAGCAAGCA CAGGGCCTTC GAGGACGAGA TGAGCGGGCG CAGCGGCCAC 
TTCGAGCAGG CCATCAAGGA AGGGGAAGAC ATGATCGCCG AGGAGCACTT CGGGGCGGAG 
AAGATCCGGG AGCGGATCGG CTACATCCGG GAACAGTGGG CCAACCTGGA GCAGCTGTCG 
GCCATCCGGA AGAAGCGCCT GGAGGAGGCC TCTCTGCTCC ACCAGTTCCA GGCGGACGCG 
GACGACATCG ACGCCTGGAT GCTGGACATC CTCAAGATCG TGTCCAGCAA CGACGTGGGC 
CACGACGAGT ACTCGACCCA GTCCCTGGTC AAGAAGCACA AGGACGTGGC CGAGGAGATT 
GCCAACTACC GGCCGGCCAT CGACTCGCTG CACGAGCAGG CCGGGGCCCT GCCCCGGGAG 
CACGCCGAGT CTCCCGACGT GCGCGGCCGG CTGTCCGGCA TCGAGGAGCG CTACAAAGAG 
GTGGCGGAGC TCACCCGGCT CCGGAAGCAG GCCCTGCAGG ACACTCTGGC CCTCTACAAG 
ATGTTCAGCG AGGCCGACGC CTGCGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AGCATGCAGA TCCCGGAGAA GCTGGAGGAC CTCGAGGTGA TCCAGCACAG GTTCGAGAGC 
TTAGAGCCGG AAATGAACAA CCAGGCGTCC CGTGTGGCCG TGGTGAACCA GATCGCCCGA 
CAGCTGATGC ACAGCGGCCA TCCGAGCGAG AAGGAGATCA AGGCCCAGCA GGATAAGCTC 
AACACAAGGT GGAGCCAGTT CCGAGAACTG GTAGACCGGA AGAAAGACGC CCTCCTCTCG 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAACGAAA CCAAGTCCTG GATTCGGGAA 
AAGACCAAAG TCATCGAATC CACCCAGGAC CTGGGCAACG ACCTGGCGGG GGTCATGGCC 
CTGCAGAGGA AGCTGACCGG CATGGAACGG GATCTGGTGG CCATCGAGGC CAAGCTGACG 
GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCGGAGCACC CGGACCAGGC CCAGGCCATC 
CTGTCCCGCC TGGCGGAGAT CAACGACGTG TGGGAGGAAA TGAAGACCAC CCTGAAGAAC 
CGCGAGGCGT CGCTGGGGGA GGCGAGCAAG CTCCAGCAGT TCCTGCGCGA CCTGGACGAC 
TTCCAGTCCT GGCTGTCCCG CACCCAGACG GCCATCGCCT CCGAGGACAT GCCCAACACC 
ATGGCCGAGG CCGAGAAGCT GCTCACCCAG CACGAGAACA TCAAGAATGA AATCAACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACGCA GGGCCAGACG 
GATGCCCAGT ACATGTTCCT GAGGCAGCGC CTGCAGGCTC TGGACACAGG CTGGAACGAG 
CTCCACAAGA TGTGGGAGAA CAGGCAGAAT CTCCTCTCCC AGTCTCACGC CTACCAGCTG 
TTCCTCAGGG ACACCAAGCA AGCGGAGGCA TTTCTCAATA ACCAGGAATA TGTCTTGGCC 
CATACCGAGA TGCCCACCAC CTTGGAGGCA GCAGAAGCTG CTATTAAGAA GCAAGAGGAC 
TTCATGACCA CGATGGACGC CAACGAGGAA AAGATCAACG CGGTGGTGGA AACCGGTCGA 
AGACTGGTGA GCGATGGCAA CATTAACTCC GACCGGATCC AAGAGAAAGT GGACTCCATC 
GACGACAGAC ATCGGAAGAA CCGGGAGGCA GCCAGCGAGC TTCTGATGCG ATTAAAAGAT 
AACAGGGATC TTCAGAAATT TCTGCAGGAT TGCCAGGAGC TGTCCCTCTG GATCAACGAG 
AAGATGCTTA CAGCCCAGGA CATGTCCTAC GACGAAGCCA GAAACCTGCA CAGTAAATGG 
CTGAAGCACC AAGCGTTCAT GGCAGAACTG GCTTCCAACA AAGAGTGGCT CGACAAAATC 
GAACAGGAAG GAATGCAGCT GATTGCCGAG AAGCCCGAGA CAGAAGCAGT GGTGAAGGAG 
AAGCTGACGG GCTTACATAA GATGTGGGAA GTTCTGGAAT CCACCACCCA GACGAAGGCC 
CAGCGGCTGT TCGATGCAAA TAAAGCCGAA CTCTTCACGC AGAGCTGTGC CGATCTGGAC 
AAGTGGCTGA ATGGCCTGGA GAGCCAGATT CAGTCTGACG ACTACGGCAA AGACCTCACC 
AGCGTAAACA TCTTACTGAA AAAGCAGCAG ATGCTGGAGA ATCAGATGGA TGTGCGGAAG 
AAGGAGATTG AGGAACTGCA GAGCCAAGCC CAGGCCTTGA GCCAAGAAGG AAAAAGCACT 
GACGAAGTGG ACAGCAAACG CCTCACCGTG CAGACCAAAT TCATGGAGCT CCTGGAGCCT 
CTGACCGAAC GGAAGAGCAA CCTGTTGGCT TCCAAAGAGA TTCATCAGTT CAACCGGGAT 
GTGGAGGACG AGATTCTGTG GGTCGGTGAA AGGATGCCCA TAGCCACCTC CACGGACCAT 
GGCCACAACC TCCAGACCGT GCAGCTGTTG ATTAAGAAGA ACCAGACCCT CCAGAAAGAA 
ATCCAGGGCC ACCAGCCCCG CATCGACGAC ATCTACGAGA GGAGTCAGAA CATCCTCACC 
GACAGCAGCC TGAGCGCCGA AGCCATCAGG CAGAGGCTGG CGGACCTGAA GCAGCTGTGG 
AACCTGGTCA TAGAGGAGAC GGAGAAGCGG CACCGGCGGC TGGAGGAATC CCACAAAGCT 
CAGCAGTACT ACTTCGACGC GGCCGAGGCC GAGGCCTGGA TGAGCGAGCA GGAGCTCTAC 
ATGATGTCAG AAGAGAAAGC CAAGGATGAG CAGAGCGCCG TGTCTATGCT GAAGAAGCAC 
CAGATTCTGG AACAAGCGGT GGAGGACTAC GCCGAGACCG TGCACCAGCT GTCCAAGACC 
AGCAGGGCTC TGGTGGCCGA CAATCACCCG GAGAGTGAGA GAATCAGCAT GAGGCAGTCC 
AAAGTGGATA AACTGTACGC AGGCCTGAAG GATTTGGCCG AGGAGAGGAG AGGCAAACTG 
GACGAGAGAC ACAGGCTATT CCAGCTGAAC CGGGAGGTGG ACGATCTGGA GCAGTGGATC 
GCCGAGAGGG AGGTGGTGGC TGGCTCCCAT GAGCTGGGAC AGGACTACGA GCACGTCACA 
ATGCTCCAAG AACGATTCCG TGAATTTGCC CGCGACACCG GAAACATCGG GCAGGAGCGG 
GTGGACACGG TCAATCACAT GGCCGACGAC CTCATCAACT CCGGACATTC CGACGCCGCC 
ACCATCGCCG AGTGGAAGGA CGGCCTGAAC GAGGCCTGGG CCGACCTCCT GGAGCTGATC 
GACACGAGAA CGCAGATCCT GGCCGCCTCG TACGAACTGC ACAAATTTTA CCACGATGCC 
AAGGAGATTT TCGGGCGCAT CCAGGACAAA CACAAGAAGC TGCCCGAGGA GCTCGGGAGG 
GATCAGAACA CGGTGGAGAC GCTCCAGAGG ATGCATACGA CATTTGAGCA TGACATCCAG 
GCTTTGGGCA CCCAGGTGCG GCAGCTGCAG GAAGATGCCG CCCGGCTCCA GGCCGCCTAC 
GCTGGAGACA AGGCCGACGA CATCCAGAAG CGGGAGAATG AGGTCCTGGA AGCCTGGAAA 
GCCCTCCTGG ACGCCTGCGA GGGCCGCAGG GCCCGGCTGG TGGACACCGG AGACAAGTTC 
CGCTTCTTCA GCTTGGTGCG CGACCTCATG CTCTGGATGG AGGACGTCAT CCGGCAGATC 
GAAGCCCAGG AGAAGCCCCG GGATGTGTCG TCTGTGGAAT TGCTCATGAA CAACCACCAG 
GGTATCAAAG CGGAAATTGA CGCCCGGAAC GACAGCTTCA CTACCTGCAT CGAGCTGGGA 
AAATCACTGT TGGCAAGGAA ACACTATGCG TCGGAGGAGA TCAAGGAGAA GCTGTTGCAG 
TTGACGGAAA AGAGAAAAGA GATGATTGAC AAGTGGGAAG ACCGATGGGA GTGGTTAAGG 
CTGATCCTGG AGGTGCACCA GTTTTCGAGG GACGCCAGCG TGGCCGAGGC CTGGCTTCTG 
GGCCAGGAGC CCTACCTGTC CAGCCGCGAG ATCGGCCAGA GCGTGGACGA GGTGGAGAAG 
CTCATCAAGC GGCACGAGGC CTTCGAGAAA TCGGCTGCCA CGTGGGACGA ACGGTTCTCG 
GCGCTGGAGA GGCTGACGAC GTTGGAATTA CTGGAAGTGC GCAGACAGCA AGAGGAAGAG 
GAGAGAAAGA GGCAGCCTCC TTCTCCGGAG CCGAGTACAA AGGTGTCAGA GGAAGCCGAA 
TCCCAGCAGC AGTGGGATGC TTCGAAAGGA GACCAAGTTT CCCAAAACGG TTTGCCTGCC 
GAGCAGGGAT CTCCACGGGT TAGTTACCGT TCTCAAACCT ACCAAAACTA CAAAAACTTT 
AATAGCAGAT GGACAGCCAG TGACCGCCCC AGGTCTGGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028927665.1
CDS	20..6484
Translation	MELQRTAGTAAPPSPAYTGQVPYNYNQLEGRFKQLQDEREAVQKKTFTKWVNSHLARVSCRITDLYADLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVAKELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMDFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEQILSSDDYGKDLTSVVRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGAEKIRERIGYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEIANYRPAIDSLHEQAGALPREHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNSMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLTDLQKEAEKLESEHPDQAQAILSRLAEINDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTMAEAEKLLTQHENIKNEINNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQLFLRDTKQAEAFLNNQEYVLAHTEMPTTLEAAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEQEGMQLIAEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLNGLESQIQSDDYGKDLTSVNILLKKQQMLENQMDVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLTERKSNLLASKEIHQFNRDVEDEILWVGERMPIATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIYERSQNILTDSSLSAEAIRQRLADLKQLWNLVIEETEKRHRRLEESHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADNHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADDLINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKALLDACEGRRARLVDTGDKFRFFSLVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRQPPSPEPSTKVSEEAESQQQWDASKGDQVSQNGLPAEQGSPRVSYRSQTYQNYKNFNSRWTASDRPRSGL

Target ORF information:

RefSeq Version	XM_029071832.1
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus spectrin beta, non-erythrocytic 1 (SPTBN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029071832.1

ORF Insert Sequence:

ATGGAATTAC AGCGGACGGC CGGCACGGCC GCACCCCCGT CCCCCGCCTA CACCGGGCAG 
GTGCCTTACA ATTACAACCA GCTGGAGGGG AGGTTCAAGC AGCTGCAAGA TGAGCGGGAA 
GCAGTGCAGA AGAAGACGTT CACCAAATGG GTCAACTCAC ACCTGGCTCG GGTGTCTTGC 
CGAATCACCG ACTTGTACGC CGACCTGCGC GATGGACGGA TGCTCATCAA ATTGCTCGAG 
GTTCTGTCCG GAGAGAGACT CCCCAAACCA ACCAAAGGAA GAATGCGTAT CCACTGCCTG 
GAGAATGTGG ACAAAGCCCT CCAGTTCTTG AAGGAGCAGC GAGTGCACCT GGAAAACATG 
GGCTCGCACG ATATCGTCGA TGGAAACCAC CGGCTGACGC TGGGCCTCAT CTGGACCATC 
ATCCTGCGTT TCCAGATCCA AGATATCAGT GTGGAAACAG AAGATAATAA GGAGAAGAAA 
TCAGCCAAGG ACGCACTGCT GCTCTGGTGC CAGATGAAGA CGGCAGGTTA CCCCAATGTC 
AACATCCATA ACTTCACCAC AAGCTGGAGA GACGGAATGG CTTTCAACGC ACTAATACAC 
AAACACCGGC CTGACCTGAT TGATTTTGAT AAGCTGAAGA AGTCAAATGC ACACTACAAT 
CTGCAGAACG CTTTTAATTT GGCTGAGCAG CACCTTGGAC TTACAAAGCT ATTGGACCCA 
GAGGATATCA GCGTTGACCA TCCTGACGAG AAGTCCATCA TCACCTACGT GGTGACATAT 
TACCACTACT TTTCCAAGAT GAAGGCTCTG GCTGTTGAAG GAAAGCGAAT TGGAAAGGTG 
CTGGACAACG CTATCGAAAC AGAGAAGATG ATCGAAAAAT ACGAATCACT CGCCTCTGAC 
CTCCTGGAAT GGATCGAACA GACCATCATT ATCTTGAACA ATCGCAAATT TGCCAACTCA 
CTGGTGGGAG TCCAGCAGCA GCTCCAGGCG TTCAACACAT ACCGCACAGT GGAGAAGCCG 
CCCAAATTTA CAGAAAAAGG GAACCTGGAA GTTCTGCTGT TCACCATCCA GAGCAAAATG 
AGGGCCAACA ATCAGAAAGT GTACATGCCC CGGGAGGGGA AGCTCATCTC CGACATCAAC 
AAGGCCTGGG AACGACTGGA AAAAGCTGAG CACGAACGAG AACTGGCACT GCGCAATGAG 
CTCATCCGAC AGGAGAAACT GGAACAACTG GCCCGCCGAT TTGATCGAAA GGCAGCTATG 
AGGGAGACGT GGCTGAGTGA AAACCAGCGT CTCGTGTCTC AGGACAACTT CGGGTTTGAC 
CTCCCCGCCG TGGAGGCCGC CACGAAAAAG CACGAGGCCA TCGAGACGGA CATCGCCGCC 
TACGAGGAGC GAGTCCAGGC CGTGGTGGCC GTGGCCAAGG AGCTGGAGGC AGAGAATTAT 
CACGACATCA AGCGAATCAC AGCGAGGAAG GACAACGTGA TCCGCCTGTG GGAGTACCTG 
CTGGAGCTGC TCCGAGCCCG TAGGCAGCGC CTGGAGATGA ACCTGGGGCT GCAGAAGATA 
TTCCAGGAAA TGCTCTACAT TATGGACTGG ATGGATGAGA TGAAGGTGCT TTTGCTGTCT 
CAAGACTATG GCAAACATTT GCTGGGGGTA GAAGACCTGT TGCAGAAACA CGCACTGGTG 
GAGGCGGACA TTGGTATCCA AGCAGAAAGG GTGAGAGGTG TCAACGCCTC GGCTCAGAAG 
TTTGCCACAG ATGGGGAAGG TTACAAGCCC TGCGACCCCC AGGTGATCCG CGACCGCGTG 
GCGCACATGG ACTTCTGCTA CCAGGAGCTG TGCCAGCTGG CGGCCGAGCG CCGGGCCCGC 
CTGGAGGAGT CCCGCCGCCT CTGGAAGTTC TTCTGGGAGA TGGCGGAAGA GGAGGGCTGG 
ATCAGGGAGA AGGAGCAGAT CCTGTCCTCG GACGACTATG GCAAAGACCT GACCAGCGTC 
GTGCGCCTGC TCAGCAAGCA CAGGGCCTTC GAGGACGAGA TGAGCGGGCG CAGCGGCCAC 
TTCGAGCAGG CCATCAAGGA AGGGGAAGAC ATGATCGCCG AGGAGCACTT CGGGGCGGAG 
AAGATCCGGG AGCGGATCGG CTACATCCGG GAACAGTGGG CCAACCTGGA GCAGCTGTCG 
GCCATCCGGA AGAAGCGCCT GGAGGAGGCC TCTCTGCTCC ACCAGTTCCA GGCGGACGCG 
GACGACATCG ACGCCTGGAT GCTGGACATC CTCAAGATCG TGTCCAGCAA CGACGTGGGC 
CACGACGAGT ACTCGACCCA GTCCCTGGTC AAGAAGCACA AGGACGTGGC CGAGGAGATT 
GCCAACTACC GGCCGGCCAT CGACTCGCTG CACGAGCAGG CCGGGGCCCT GCCCCGGGAG 
CACGCCGAGT CTCCCGACGT GCGCGGCCGG CTGTCCGGCA TCGAGGAGCG CTACAAAGAG 
GTGGCGGAGC TCACCCGGCT CCGGAAGCAG GCCCTGCAGG ACACTCTGGC CCTCTACAAG 
ATGTTCAGCG AGGCCGACGC CTGCGAGCTC TGGATCGACG AGAAGGAGCA GTGGCTCAAC 
AGCATGCAGA TCCCGGAGAA GCTGGAGGAC CTCGAGGTGA TCCAGCACAG GTTCGAGAGC 
TTAGAGCCGG AAATGAACAA CCAGGCGTCC CGTGTGGCCG TGGTGAACCA GATCGCCCGA 
CAGCTGATGC ACAGCGGCCA TCCGAGCGAG AAGGAGATCA AGGCCCAGCA GGATAAGCTC 
AACACAAGGT GGAGCCAGTT CCGAGAACTG GTAGACCGGA AGAAAGACGC CCTCCTCTCG 
GCCCTGAGCA TCCAGAACTA CCACCTCGAG TGCAACGAAA CCAAGTCCTG GATTCGGGAA 
AAGACCAAAG TCATCGAATC CACCCAGGAC CTGGGCAACG ACCTGGCGGG GGTCATGGCC 
CTGCAGAGGA AGCTGACCGG CATGGAACGG GATCTGGTGG CCATCGAGGC CAAGCTGACG 
GACCTGCAGA AGGAGGCGGA GAAGCTGGAG TCGGAGCACC CGGACCAGGC CCAGGCCATC 
CTGTCCCGCC TGGCGGAGAT CAACGACGTG TGGGAGGAAA TGAAGACCAC CCTGAAGAAC 
CGCGAGGCGT CGCTGGGGGA GGCGAGCAAG CTCCAGCAGT TCCTGCGCGA CCTGGACGAC 
TTCCAGTCCT GGCTGTCCCG CACCCAGACG GCCATCGCCT CCGAGGACAT GCCCAACACC 
ATGGCCGAGG CCGAGAAGCT GCTCACCCAG CACGAGAACA TCAAGAATGA AATCAACAAC 
TACGAGGAGG ACTACCAGAA GATGAGGGAC ATGGGCGAGA TGGTCACGCA GGGCCAGACG 
GATGCCCAGT ACATGTTCCT GAGGCAGCGC CTGCAGGCTC TGGACACAGG CTGGAACGAG 
CTCCACAAGA TGTGGGAGAA CAGGCAGAAT CTCCTCTCCC AGTCTCACGC CTACCAGCTG 
TTCCTCAGGG ACACCAAGCA AGCGGAGGCA TTTCTCAATA ACCAGGAATA TGTCTTGGCC 
CATACCGAGA TGCCCACCAC CTTGGAGGCA GCAGAAGCTG CTATTAAGAA GCAAGAGGAC 
TTCATGACCA CGATGGACGC CAACGAGGAA AAGATCAACG CGGTGGTGGA AACCGGTCGA 
AGACTGGTGA GCGATGGCAA CATTAACTCC GACCGGATCC AAGAGAAAGT GGACTCCATC 
GACGACAGAC ATCGGAAGAA CCGGGAGGCA GCCAGCGAGC TTCTGATGCG ATTAAAAGAT 
AACAGGGATC TTCAGAAATT TCTGCAGGAT TGCCAGGAGC TGTCCCTCTG GATCAACGAG 
AAGATGCTTA CAGCCCAGGA CATGTCCTAC GACGAAGCCA GAAACCTGCA CAGTAAATGG 
CTGAAGCACC AAGCGTTCAT GGCAGAACTG GCTTCCAACA AAGAGTGGCT CGACAAAATC 
GAACAGGAAG GAATGCAGCT GATTGCCGAG AAGCCCGAGA CAGAAGCAGT GGTGAAGGAG 
AAGCTGACGG GCTTACATAA GATGTGGGAA GTTCTGGAAT CCACCACCCA GACGAAGGCC 
CAGCGGCTGT TCGATGCAAA TAAAGCCGAA CTCTTCACGC AGAGCTGTGC CGATCTGGAC 
AAGTGGCTGA ATGGCCTGGA GAGCCAGATT CAGTCTGACG ACTACGGCAA AGACCTCACC 
AGCGTAAACA TCTTACTGAA AAAGCAGCAG ATGCTGGAGA ATCAGATGGA TGTGCGGAAG 
AAGGAGATTG AGGAACTGCA GAGCCAAGCC CAGGCCTTGA GCCAAGAAGG AAAAAGCACT 
GACGAAGTGG ACAGCAAACG CCTCACCGTG CAGACCAAAT TCATGGAGCT CCTGGAGCCT 
CTGACCGAAC GGAAGAGCAA CCTGTTGGCT TCCAAAGAGA TTCATCAGTT CAACCGGGAT 
GTGGAGGACG AGATTCTGTG GGTCGGTGAA AGGATGCCCA TAGCCACCTC CACGGACCAT 
GGCCACAACC TCCAGACCGT GCAGCTGTTG ATTAAGAAGA ACCAGACCCT CCAGAAAGAA 
ATCCAGGGCC ACCAGCCCCG CATCGACGAC ATCTACGAGA GGAGTCAGAA CATCCTCACC 
GACAGCAGCC TGAGCGCCGA AGCCATCAGG CAGAGGCTGG CGGACCTGAA GCAGCTGTGG 
AACCTGGTCA TAGAGGAGAC GGAGAAGCGG CACCGGCGGC TGGAGGAATC CCACAAAGCT 
CAGCAGTACT ACTTCGACGC GGCCGAGGCC GAGGCCTGGA TGAGCGAGCA GGAGCTCTAC 
ATGATGTCAG AAGAGAAAGC CAAGGATGAG CAGAGCGCCG TGTCTATGCT GAAGAAGCAC 
CAGATTCTGG AACAAGCGGT GGAGGACTAC GCCGAGACCG TGCACCAGCT GTCCAAGACC 
AGCAGGGCTC TGGTGGCCGA CAATCACCCG GAGAGTGAGA GAATCAGCAT GAGGCAGTCC 
AAAGTGGATA AACTGTACGC AGGCCTGAAG GATTTGGCCG AGGAGAGGAG AGGCAAACTG 
GACGAGAGAC ACAGGCTATT CCAGCTGAAC CGGGAGGTGG ACGATCTGGA GCAGTGGATC 
GCCGAGAGGG AGGTGGTGGC TGGCTCCCAT GAGCTGGGAC AGGACTACGA GCACGTCACA 
ATGCTCCAAG AACGATTCCG TGAATTTGCC CGCGACACCG GAAACATCGG GCAGGAGCGG 
GTGGACACGG TCAATCACAT GGCCGACGAC CTCATCAACT CCGGACATTC CGACGCCGCC 
ACCATCGCCG AGTGGAAGGA CGGCCTGAAC GAGGCCTGGG CCGACCTCCT GGAGCTGATC 
GACACGAGAA CGCAGATCCT GGCCGCCTCG TACGAACTGC ACAAATTTTA CCACGATGCC 
AAGGAGATTT TCGGGCGCAT CCAGGACAAA CACAAGAAGC TGCCCGAGGA GCTCGGGAGG 
GATCAGAACA CGGTGGAGAC GCTCCAGAGG ATGCATACGA CATTTGAGCA TGACATCCAG 
GCTTTGGGCA CCCAGGTGCG GCAGCTGCAG GAAGATGCCG CCCGGCTCCA GGCCGCCTAC 
GCTGGAGACA AGGCCGACGA CATCCAGAAG CGGGAGAATG AGGTCCTGGA AGCCTGGAAA 
GCCCTCCTGG ACGCCTGCGA GGGCCGCAGG GCCCGGCTGG TGGACACCGG AGACAAGTTC 
CGCTTCTTCA GCTTGGTGCG CGACCTCATG CTCTGGATGG AGGACGTCAT CCGGCAGATC 
GAAGCCCAGG AGAAGCCCCG GGATGTGTCG TCTGTGGAAT TGCTCATGAA CAACCACCAG 
GGTATCAAAG CGGAAATTGA CGCCCGGAAC GACAGCTTCA CTACCTGCAT CGAGCTGGGA 
AAATCACTGT TGGCAAGGAA ACACTATGCG TCGGAGGAGA TCAAGGAGAA GCTGTTGCAG 
TTGACGGAAA AGAGAAAAGA GATGATTGAC AAGTGGGAAG ACCGATGGGA GTGGTTAAGG 
CTGATCCTGG AGGTGCACCA GTTTTCGAGG GACGCCAGCG TGGCCGAGGC CTGGCTTCTG 
GGCCAGGAGC CCTACCTGTC CAGCCGCGAG ATCGGCCAGA GCGTGGACGA GGTGGAGAAG 
CTCATCAAGC GGCACGAGGC CTTCGAGAAA TCGGCTGCCA CGTGGGACGA ACGGTTCTCG 
GCGCTGGAGA GGCTGACGAC GTTGGAATTA CTGGAAGTGC GCAGACAGCA AGAGGAAGAG 
GAGAGAAAGA GGCAGCCTCC TTCTCCGGAG CCGAGTACAA AGGTGTCAGA GGAAGCCGAA 
TCCCAGCAGC AGTGGGATGC TTCGAAAGGA GACCAAGTTT CCCAAAACGG TTTGCCTGCC 
GAGCAGGGAT CTCCACGGGT TAGTTACCGT TCTCAAACCT ACCAAAACTA CAAAAACTTT 
AATAGCAGAT GGACAGCCAG TGACCGCCCC AGGTCTGGAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTBN1 ( XM_003431278.3 ) cDNA ORF clone, Ornithorhynchus anatinus(platypus) -> XP_003431326.1 Ornithorhynchus anatinus spectrin beta, non-erythrocytic 1 (SPTBN1), mRNA.

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CloneID	OOc13690
Clone ID Related Accession (Same CDS sequence)	XM_003431278.3
Accession Version	XM_003431278.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1460563200000
Organism	Ornithorhynchus anatinus(platypus)
Product	LOW QUALITY PROTEIN: spectrin beta chain, non-erythrocytic 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001794503.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 15, 2016 this sequence version replaced XM_003431278.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ornithorhynchus anatinus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGACGACGA CGGTGGCCAC AGATTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAC 
GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAAA ACAGCTCCGC CCGGCTCTTT 
GAGCGATCCC GAATCAAGGC GCTAGCAGAT GAGCGGGAAG CAGTGCAGAA GAAGACGTTC 
ACCAAATGGG TCAACTCACA CCTGGCTCGG GTGTCTTGCC GAATCACCGA CTTGTACGCC 
GACCTGCGCG ATGGACGGAT GCTCATCAAA TTGCTCGAGG TTCTGTCCGG AGAGAGACTC 
CCCAAACCAA CCAAAGGAAG AATGCGTATC CACTGCCTGG AGAATGTGGA CAAAGCCCTC 
CAGTTCTTGA AGGAGCAGCG AGTGCACCTG GAAAACATGG GCTCGCACGA TATCGTCGAT 
GGAAACCACC GGCTGACGCT GGGCCTCATC TGGACCATCA TCCTGCGTTT CCAGATCCAA 
GATATCAGTG TGGAAACAGA AGATAATAAG GAGAAGAAAT CAGCCAAGGA CGCACTGCTG 
CTCTGGTGCC AGATGAAGAC GGCAGGTTAC CCCAATGTCA ACATCCATAA CTTCACCACA 
AGCTGGAGAG ACGGAATGGC TTTCAACGCA CTAATACACA AACACCGGCC TGACCTGATT 
GATTTTGATA AGCTGAAGAA GTCAAATGCA CACTACAATC TGCAGAACGC TTTTAATTTG 
GCTGAGCAGC ACCTTGGACT TACAAAGCTA TTGGACCCAG AGGATATCAG CGTTGACCAT 
CCTGACGAGA AGTCCATCAT CACCTACGTG GTGACATATT ACCACTACTT TTCCAAGATG 
AAGGCTCTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TGGACAACGC TATCGAAACA 
GAGAAGATGA TCGAAAAATA CGAATCACTC GCCTCTGACC TCCTGGAATG GATCGAACAG 
ACCATCATTA TCTTGAACAA TCGCAAATTT GCCAACTCAC TGGTGGGAGT CCAGCAGCAG 
CTCCAGGCGT TCAACACATA CCGCACAGTG GAGAAGCCGC CCAAATTTAC AGAAAAAGGG 
AACCTGGAAG TTCTGCTGTT CACCATCCAG AGCAAAATGA GGGCCAACAA TCAGAAAGTG 
TACATGCCCC GGGAGGGGAA GCTCATCTCC GACATCAACA AGGCCTGGGA ACGACTGGAA 
AAAGCTGAGC ACGAACGAGA ACTGGCACTG CGCAATGAGC TCATCCGACA GGAGAAACTG 
GAACAACTGG CCCGCCGATT TGATCGAAAG GCAGCTATGA GGGAGACGTG GCTGAGTGAA 
AACCAGCGTC TCGTGTCTCA GGACAACTTC GGGTTTGACC TCCCCGCCGT GGAGGCCGCC 
ACGAAAAAGC ACGAGGCCAT CGAGACGGAC ATCGCCGCCT ACGAGGAGCG AGTCCAGGCC 
GTAGTGGCCG TGGCCAAGGA GCTGGAGGCA GAGAATTATC ACGACATCAA GCGAATCACA 
GCGAGGAAGG ACAACGTGAT CCGCCTGTGG GAGTACCTGC TGGAGCTGCT CCGAGCCCGT 
AGGCAGCGCC TGGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAGAT GAAGGTGCTT TTGCTGTCTC AAGACTATGG CAAACATTTG 
CTGGGGGTAG AAGACCTGTT GCAGAAACAC GCACTGGTGG AGGCGGACAT TGGTATCCAA 
GCAGAAAGGG TGAGAGGTGT CAACGCCTCG GCTCAGAAGT TTGCCACAGA TGGGGAAGGT 
TACAAGCCCT GCGACCCCCA GGTGATCCGC GACCGCGTGG CGCACATGGA CTTCTGCTAC 
CAGGAGCTGT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAGGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCGGAAGAG GAGGGCTGGA TCAGGGAGAA GGAGCAGATC 
CTGTCCTCGG ACGACTATGG CAAAGACCTG ACCAGCGTCG TGCGCCTGCT CAGCAAGCAC 
AGGGCCTTCG AGGACGAGAT GAGCGGGCGC AGCGGCCACT TCGAGCAGGC CATCAAGGAA 
GGGGAAGACA TGATCGCCGA GGAGCACTTC GGGGCGGAGA AGATCCGGGA GCGGATCGGC 
TACATCCGGG AACAGTGGGC CAACCTGGAG CAGCTGTCGG CCATCCGGAA GAAGCGCCTG 
GAGGAGGCCT CTCTGCTCCA CCAGTTCCAG GCGGACGCGG ACGACATCGA CGCCTGGATG 
CTGGACATCC TCAAGATCGT GTCCAGCAAC GACGTGGGCC ACGACGAGTA CTCGACCCAG 
TCCCTGGTCA AGAAGCACAA GGACGTGGCC GAGGAGATTG CCAACTACCG GCCGGCCATC 
GACTCGCTGC ACGAGCAGGC CGGGGCCCTG CCCCGGGAGC ACGCCGAGTC GCCCGACGTG 
CGCGGCCGGC TGTCCGGCAT CGAGGAGCGC TACAAAGAGG TGGCGGAGCT CACCCGGCTC 
CGGAAGCAGG CCCTGCAGGA CACTCTGGCC CTCTACAAGA TGTTCAGCGA GGCCGACGCC 
TGCGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA GCATGCAGAT CCCGGAGAAG 
CTGGAGGACC TCGAGGTGAT CCAGCACAGG TTCGAGAGCT TAGAGCCGGA AATGAACAAC 
CAGGCGTCCC GTGTGGCCGT GGTGAACCAG ATCGCCCGAC AGCTGATGCA CAGCGGCCAT 
CCGAGCGAGA AGGAGATCAA GGCCCAGCAG GATAAGCTCA ACACAAGGTG GAGCCAGTTC 
CGAGAACTGG TAGACCGGAA GAAAGACGCC CTCCTCTCGG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAACGAAAC CAAGTCCTGG ATTCGGGAAA AGACCAAAGT CATCGAATCC 
ACCCAGGACC TGGGCAACGA CCTGGCGGGG GTCATGGCCC TGCAGAGGAA GCTGACCGGC 
ATGGAACGGG ATCTGGTGGC CATCGAGGCC AAGCTGACGG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CGGAGCACCC GGACCAGGCC CAGGCCATCC TGTCCCGCCT GGCGGAGATC 
AACGACGTGT GGGAGGAAAT GAAGACCACC CTGAAGAACC GCGAGGCGTC GCTGGGGGAG 
GCGAGCAAGC TCCAGCAGTT CCTGCGCGAC CTGGACGACT TCCAGTCCTG GCTGTCCCGC 
ACCCAGACGG CCATCGCCTC CGAGGACATG CCCAACACCA TGGCCGAGGC CGAGAAGCTG 
CTCACCCAGC ACGAGAACAT CAAGAATGAA ATCAACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACGCAG GGCCAGACGG ATGCCCAGTA CATGTTCCTG 
AGGCAGCGCC TGCAGGCTCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGGCAGAATC TCCTCTCCCA GTCTCACGCC TACCAGCTGT TCCTCAGGGA CACCAAGCAA 
GCGGAGGCAT TTCTCAATAA CCAGGAATAT GTCTTGGCCC ATACCGAGAT GCCCACCACC 
TTGGAGGCAG CAGAAGCTGC TATTAAGAAG CAAGAGGACT TCATGACCAC GATGGACGCC 
AACGAGGAAA AGATCAACGC GGTGGTGGAA ACCGGTCGAA GACTGGTGAG CGATGGCAAC 
ATTAACTCCG ACCGGATCCA AGAGAAAGTG GACTCCATCG ACGACAGACA TCGGAAGAAC 
CGGGAGGCAG CCAGCGAGCT TCTGATGCGA TTAAAAGATA ACAGGGATCT TCAGAAATTT 
CTGCAGGATT GCCAGGAGCT GTCCCTCTGG ATCAACGAGA AGATGCTTAC AGCCCAGGAC 
ATGTCCTACG ACGAAGCCAG AAACCTGCAC AGTAAATGGC TGAAGCACCA AGCGTTCATG 
GCAGAACTGG CTTCCAACAA AGAGTGGCTC GACAAAATCG AACAGGAAGG AATGCAGCTG 
ATTGCCGAGA AGCCCGAGAC AGAAGCAGTG GTGAAGGAGA AGCTGACGGG CTTACATAAG 
ATGTGGGAAG TTCTGGAATC CACCACCCAG ACGAAGGCCC AGCGGCTGTT CGATGCAAAT 
AAAGCCGAAC TCTTCACGCA GAGCTGTGCC GATCTGGACA AGTGGCTGAA TGGCCTGGAG 
AGCCAGATTC AGTCTGACGA CTACGGCAAA GACCTCACCA GCGTAAACAT CTTACTGAAA 
AAGCAGCAGA TGCTGGAGAA TCAGATGGAT GTGCGGAAGA AGGAGATTGA GGAACTGCAG 
AGCCAAGCCC AGGCCTTGAG CCAAGAAGGA AAAAGCACTG ACGAAGTGGA CAGCAAACGC 
CTCACCGTGC AGACCAAATT CATGGAGCTC CTGGAGCCTC TGACCGAACG GAAGAGCAAC 
CTGTTGGCTT CCAAAGAGAT TCATCAGTTC AACCGGGATG TGGAGGACGA GATTCTGTGG 
GTCGGTGAAA GGATGCCCAT AGCCACCTCC ACGGACCATG GCCACAACCT CCAGACCGTG 
CAGCTGTTGA TTAAGAAGAA CCAGACCCTC CAGAAAGAAA TCCAGGGCCA CCAGCCCCGC 
ATCGACGACA TCTACGAGAG GAGTCAGAAT ATCCTCACCG ACAGCAGCCT GAGCGCCGAA 
GCCATCAGGC AGAGGCTGGC GGACCTGAAG CAGCTGTGGA ACCTGGTCAT AGAGGAGACG 
GAAAAGCGGC ACCGGCGGCT GGAGGAATCC CACAAAGCTC AGCAGTACTA CTTCGATGCG 
GCCGAGGCCG AGGCCTGGAT GAGCGAGCAG GAGCTCTACA TGATGTCGGA AGAGAAAGCC 
AAGGATGAGC AGAGCGCCGT GTCTATGCTG AAGAAGCACC AGATTCTGGA ACAAGCGGTG 
GAGGACTACG CCGAGACCGT GCACCAGCTG TCCAAGACCA GCAGGGCTCT GGTGGCCGAC 
AATCACCCGG AGAGTGAGAG AATCAGCATG AGGCAGTCCA AAGTGGATAA ACTGTACGCA 
GGCCTCAAGG ATTTGGCCGA GGAGAGGAGA GGCAAACTGG ACGAGAGACA CAGGCTATTC 
CAGCTGAACC GGGAGGTGGA CGATCTGGAG CAGTGGATCG CCGAGAGGGA GGTGGTGGCT 
GGCTCCCATG AGCTGGGACA GGACTACGAG CACGTCACAA TGCTCCAAGA ACGATTCCGT 
GAATTTGCCC GCGACACCGG AAACATCGGG CAGGAGCGGG TGGACACGGT CAATCACATG 
GCCGACGACC TCATCAACTC CGGACATTCC GACGCCGCCA CCATCGCCGA GTGGAAGGAC 
GGCCTGAACG AGGCCTGGGC CGACCTCCTG GAGCTGATCG ACACGAGAAC GCAGATCCTG 
GCCGCCTCGT ACGAACTGCA CAAATTTTAC CACGATGCCA AGGAGATCTT CGGGCGCATC 
CAGGACAAAC ACAAGAAGCT GCCCGAGGAG CTCGGGAGGG ATCAGAACAC GGTGGAGACG 
CTCCAGAGGA TGCATACGAC ATTTGAGCAT GACATCCAGG CTTTGGGCAC CCAGGTGCGG 
CAGCTGCAGG AAGATGCCGC CCGGCTCCAG GCCGCCTACG CTGGAGACAA GGCCGACGAC 
ATCCAGAAGC GGGAGAATGA GGTCCTGGAA GCCTGGAAAG CCCTCCTGGA CGCCTGCGAG 
GGCCGCAGGN CCCGGCTGGT GGACACCGGA GACAAGTTCC GCTTCTTCAG CTTGGTGCGC 
GACCTCATGC TCTGGATGGA GGACGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG 
GATGTGTCGT CTGTGGAATT GCTCATGAAC AACCACCAGG GTATCAAAGC GGAAATTGAC 
GCCCGGAACG ACAGCTTCAC TACCTGCATC GAGCTGGGAA AATCACTGTT GGCAAGGAAA 
CACTATGCGT CGGAGGAGAT CAAGGAGAAG CTGTTGCAGT TGACGGAAAA GAGAAAAGAG 
ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGGC TGATCCTGGA GGTGCACCAG 
TTTTCGAGGG ACGCCAGCGT GGCCGAGGCC TGGCTTCTGG GCCAGGAGCC CTACCTGTCC 
AGCCGCGAGA TCGGCCAGAG CGTGGACGAG GTGGAGAAGC TCATCAAGCG GCACGAGGCC 
TTCGAGAAAT CGGCTGCCAC GTGGGACGAA CGGTTCTCGG CGCTGGAGAG GCTGACGACG 
TTGGAATTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGAAAGAG GCAGCCTCCT 
TCTCCGGAGC CGAGTACAAA GGTGTCAGAG GAAGCCGAAT CCCAGCAGCA GTGGGATGCT 
TCGAAAGGAG ACCAAGTTTC CCAAAACGGT TTGCCTGCCG AGCAGGGATC TCCACGGCTG 
GCGGAAACTG CCGAAACGAG TGAGATGGTC AACGGAGCCA CCGAGCAGAG GACGAGTTCC 
AAAGAATCCA GTCCGGTTCC TTCCCCGACT TCGGATCGTA AAGCCAAGAC CGTCCTCCAG 
GCCCAGACGG CCGCCACCTT GCCCGCCAAG ACCCAGGAGG TCCCGGCGGC CCAGATGGAG 
GGCTTCTTGA ATCGCAAGCA CGAGTGGGAG GCCCACAATA AGAAAGCCTC CAGCAGGTCT 
TGGCACAACG TATACTGTGT CATCAACAAC CAAGAGATGG GCTTCTACAA GGATGCGAAG 
GCCGCTTCTT CTGGGATACC GTACCACAAC GAGATTCCAG TTAGTTTGAA AGAAGCAGTG 
TGTGAAGTAG CCCTTGACTA CAAAAAGAAA AAGCACGTGT TTAAACTAAG ACTAAATGAC 
GGCAATGAAT ACCTTTTCCA AGCCAAAGAC GATGAGGAAA TGTCCACGTG GATCCAGGCC 
ATCACGTCCG CCATCTCCTC AGACAAGATG GACGTGTCCG CCAGCACCCA GAGCACTCCA 
GCCTCCAGCC GGGCCCAGAC GCTGCCCGCC AGCGTCACAA TAACCAGCGA GTCCAGTCCC 
GGCAAGCGGG AGAAGGACAA GGAAAAGGAC AAAGAGAAAA GGTTCAGCCT CTTTGGCAAA 
AAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003431326.1
CDS	171..7259
Translation	MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLARVSCRITDLYADLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVDGNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNALIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKMKALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTVEKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKLEQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVAKELEAENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHLLGVEDLLQKHALVEADIGIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMDFCYQELCQLAAERRARLEESRRLWKFFWEMAEEEGWIREKEQILSSDDYGKDLTSVVRLLSKHRAFEDEMSGRSGHFEQAIKEGEDMIAEEHFGAEKIRERIGYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSNDVGHDEYSTQSLVKKHKDVAEEIANYRPAIDSLHEQAGALPREHAESPDVRGRLSGIEERYKEVAELTRLRKQALQDTLALYKMFSEADACELWIDEKEQWLNSMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQIARQLMHSGHPSEKEIKAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIESTQDLGNDLAGVMALQRKLTGMERDLVAIEAKLTDLQKEAEKLESEHPDQAQAILSRLAEINDVWEEMKTTLKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTMAEAEKLLTQHENIKNEINNYEEDYQKMRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQLFLRDTKQAEAFLNNQEYVLAHTEMPTTLEAAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKNREAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWLDKIEQEGMQLIAEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLNGLESQIQSDDYGKDLTSVNILLKKQQMLENQMDVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMELLEPLTERKSNLLASKEIHQFNRDVEDEILWVGERMPIATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIYERSQNILTDSSLSAEAIRQRLADLKQLWNLVIEETEKRHRRLEESHKAQQYYFDAAEAEAWMSEQELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADNHPESERISMRQSKVDKLYAGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNIGQERVDTVNHMADDLINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGRIQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVLEAWKALLDACEGRRnullRLVDTGDKFRFFSLVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEIDARNDSFTTCIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAEAWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRQPPSPEPSTKVSEEAESQQQWDASKGDQVSQNGLPAEQGSPRLAETAETSEMVNGATEQRTSSKESSPVPSPTSDRKAKTVLQAQTAATLPAKTQEVPAAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAKAASSGIPYHNEIPVSLKEAVCEVALDYKKKKHVFKLRLNDGNEYLFQAKDDEEMSTWIQAITSAISSDKMDVSASTQSTPASSRAQTLPASVTITSESSPGKREKDKEKDKEKRFSLFGKKK

Target ORF information:

RefSeq Version	XM_003431278.3
Organism	Ornithorhynchus anatinus(platypus)
Definition	Ornithorhynchus anatinus spectrin beta, non-erythrocytic 1 (SPTBN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003431278.3

ORF Insert Sequence:

ATGACGACGA CGGTGGCCAC AGATTACGAC AACATTGAGA TCCAGCAGCA GTACAGCGAC 
GTCAACAACC GCTGGGACGT GGACGACTGG GACAATGAAA ACAGCTCCGC CCGGCTCTTT 
GAGCGATCCC GAATCAAGGC GCTAGCAGAT GAGCGGGAAG CAGTGCAGAA GAAGACGTTC 
ACCAAATGGG TCAACTCACA CCTGGCTCGG GTGTCTTGCC GAATCACCGA CTTGTACGCC 
GACCTGCGCG ATGGACGGAT GCTCATCAAA TTGCTCGAGG TTCTGTCCGG AGAGAGACTC 
CCCAAACCAA CCAAAGGAAG AATGCGTATC CACTGCCTGG AGAATGTGGA CAAAGCCCTC 
CAGTTCTTGA AGGAGCAGCG AGTGCACCTG GAAAACATGG GCTCGCACGA TATCGTCGAT 
GGAAACCACC GGCTGACGCT GGGCCTCATC TGGACCATCA TCCTGCGTTT CCAGATCCAA 
GATATCAGTG TGGAAACAGA AGATAATAAG GAGAAGAAAT CAGCCAAGGA CGCACTGCTG 
CTCTGGTGCC AGATGAAGAC GGCAGGTTAC CCCAATGTCA ACATCCATAA CTTCACCACA 
AGCTGGAGAG ACGGAATGGC TTTCAACGCA CTAATACACA AACACCGGCC TGACCTGATT 
GATTTTGATA AGCTGAAGAA GTCAAATGCA CACTACAATC TGCAGAACGC TTTTAATTTG 
GCTGAGCAGC ACCTTGGACT TACAAAGCTA TTGGACCCAG AGGATATCAG CGTTGACCAT 
CCTGACGAGA AGTCCATCAT CACCTACGTG GTGACATATT ACCACTACTT TTCCAAGATG 
AAGGCTCTGG CTGTTGAAGG AAAGCGAATT GGAAAGGTGC TGGACAACGC TATCGAAACA 
GAGAAGATGA TCGAAAAATA CGAATCACTC GCCTCTGACC TCCTGGAATG GATCGAACAG 
ACCATCATTA TCTTGAACAA TCGCAAATTT GCCAACTCAC TGGTGGGAGT CCAGCAGCAG 
CTCCAGGCGT TCAACACATA CCGCACAGTG GAGAAGCCGC CCAAATTTAC AGAAAAAGGG 
AACCTGGAAG TTCTGCTGTT CACCATCCAG AGCAAAATGA GGGCCAACAA TCAGAAAGTG 
TACATGCCCC GGGAGGGGAA GCTCATCTCC GACATCAACA AGGCCTGGGA ACGACTGGAA 
AAAGCTGAGC ACGAACGAGA ACTGGCACTG CGCAATGAGC TCATCCGACA GGAGAAACTG 
GAACAACTGG CCCGCCGATT TGATCGAAAG GCAGCTATGA GGGAGACGTG GCTGAGTGAA 
AACCAGCGTC TCGTGTCTCA GGACAACTTC GGGTTTGACC TCCCCGCCGT GGAGGCCGCC 
ACGAAAAAGC ACGAGGCCAT CGAGACGGAC ATCGCCGCCT ACGAGGAGCG AGTCCAGGCC 
GTAGTGGCCG TGGCCAAGGA GCTGGAGGCA GAGAATTATC ACGACATCAA GCGAATCACA 
GCGAGGAAGG ACAACGTGAT CCGCCTGTGG GAGTACCTGC TGGAGCTGCT CCGAGCCCGT 
AGGCAGCGCC TGGAGATGAA CCTGGGGCTG CAGAAGATAT TCCAGGAAAT GCTCTACATT 
ATGGACTGGA TGGATGAGAT GAAGGTGCTT TTGCTGTCTC AAGACTATGG CAAACATTTG 
CTGGGGGTAG AAGACCTGTT GCAGAAACAC GCACTGGTGG AGGCGGACAT TGGTATCCAA 
GCAGAAAGGG TGAGAGGTGT CAACGCCTCG GCTCAGAAGT TTGCCACAGA TGGGGAAGGT 
TACAAGCCCT GCGACCCCCA GGTGATCCGC GACCGCGTGG CGCACATGGA CTTCTGCTAC 
CAGGAGCTGT GCCAGCTGGC GGCCGAGCGC CGGGCCCGCC TGGAGGAGTC CCGCCGCCTC 
TGGAAGTTCT TCTGGGAGAT GGCGGAAGAG GAGGGCTGGA TCAGGGAGAA GGAGCAGATC 
CTGTCCTCGG ACGACTATGG CAAAGACCTG ACCAGCGTCG TGCGCCTGCT CAGCAAGCAC 
AGGGCCTTCG AGGACGAGAT GAGCGGGCGC AGCGGCCACT TCGAGCAGGC CATCAAGGAA 
GGGGAAGACA TGATCGCCGA GGAGCACTTC GGGGCGGAGA AGATCCGGGA GCGGATCGGC 
TACATCCGGG AACAGTGGGC CAACCTGGAG CAGCTGTCGG CCATCCGGAA GAAGCGCCTG 
GAGGAGGCCT CTCTGCTCCA CCAGTTCCAG GCGGACGCGG ACGACATCGA CGCCTGGATG 
CTGGACATCC TCAAGATCGT GTCCAGCAAC GACGTGGGCC ACGACGAGTA CTCGACCCAG 
TCCCTGGTCA AGAAGCACAA GGACGTGGCC GAGGAGATTG CCAACTACCG GCCGGCCATC 
GACTCGCTGC ACGAGCAGGC CGGGGCCCTG CCCCGGGAGC ACGCCGAGTC GCCCGACGTG 
CGCGGCCGGC TGTCCGGCAT CGAGGAGCGC TACAAAGAGG TGGCGGAGCT CACCCGGCTC 
CGGAAGCAGG CCCTGCAGGA CACTCTGGCC CTCTACAAGA TGTTCAGCGA GGCCGACGCC 
TGCGAGCTCT GGATTGACGA GAAGGAGCAG TGGCTCAACA GCATGCAGAT CCCGGAGAAG 
CTGGAGGACC TCGAGGTGAT CCAGCACAGG TTCGAGAGCT TAGAGCCGGA AATGAACAAC 
CAGGCGTCCC GTGTGGCCGT GGTGAACCAG ATCGCCCGAC AGCTGATGCA CAGCGGCCAT 
CCGAGCGAGA AGGAGATCAA GGCCCAGCAG GATAAGCTCA ACACAAGGTG GAGCCAGTTC 
CGAGAACTGG TAGACCGGAA GAAAGACGCC CTCCTCTCGG CCCTGAGCAT CCAGAACTAC 
CACCTCGAGT GCAACGAAAC CAAGTCCTGG ATTCGGGAAA AGACCAAAGT CATCGAATCC 
ACCCAGGACC TGGGCAACGA CCTGGCGGGG GTCATGGCCC TGCAGAGGAA GCTGACCGGC 
ATGGAACGGG ATCTGGTGGC CATCGAGGCC AAGCTGACGG ACCTGCAGAA GGAGGCGGAG 
AAGCTGGAGT CGGAGCACCC GGACCAGGCC CAGGCCATCC TGTCCCGCCT GGCGGAGATC 
AACGACGTGT GGGAGGAAAT GAAGACCACC CTGAAGAACC GCGAGGCGTC GCTGGGGGAG 
GCGAGCAAGC TCCAGCAGTT CCTGCGCGAC CTGGACGACT TCCAGTCCTG GCTGTCCCGC 
ACCCAGACGG CCATCGCCTC CGAGGACATG CCCAACACCA TGGCCGAGGC CGAGAAGCTG 
CTCACCCAGC ACGAGAACAT CAAGAATGAA ATCAACAACT ACGAGGAGGA CTACCAGAAG 
ATGAGGGACA TGGGCGAGAT GGTCACGCAG GGCCAGACGG ATGCCCAGTA CATGTTCCTG 
AGGCAGCGCC TGCAGGCTCT GGACACAGGC TGGAACGAGC TCCACAAGAT GTGGGAGAAC 
AGGCAGAATC TCCTCTCCCA GTCTCACGCC TACCAGCTGT TCCTCAGGGA CACCAAGCAA 
GCGGAGGCAT TTCTCAATAA CCAGGAATAT GTCTTGGCCC ATACCGAGAT GCCCACCACC 
TTGGAGGCAG CAGAAGCTGC TATTAAGAAG CAAGAGGACT TCATGACCAC GATGGACGCC 
AACGAGGAAA AGATCAACGC GGTGGTGGAA ACCGGTCGAA GACTGGTGAG CGATGGCAAC 
ATTAACTCCG ACCGGATCCA AGAGAAAGTG GACTCCATCG ACGACAGACA TCGGAAGAAC 
CGGGAGGCAG CCAGCGAGCT TCTGATGCGA TTAAAAGATA ACAGGGATCT TCAGAAATTT 
CTGCAGGATT GCCAGGAGCT GTCCCTCTGG ATCAACGAGA AGATGCTTAC AGCCCAGGAC 
ATGTCCTACG ACGAAGCCAG AAACCTGCAC AGTAAATGGC TGAAGCACCA AGCGTTCATG 
GCAGAACTGG CTTCCAACAA AGAGTGGCTC GACAAAATCG AACAGGAAGG AATGCAGCTG 
ATTGCCGAGA AGCCCGAGAC AGAAGCAGTG GTGAAGGAGA AGCTGACGGG CTTACATAAG 
ATGTGGGAAG TTCTGGAATC CACCACCCAG ACGAAGGCCC AGCGGCTGTT CGATGCAAAT 
AAAGCCGAAC TCTTCACGCA GAGCTGTGCC GATCTGGACA AGTGGCTGAA TGGCCTGGAG 
AGCCAGATTC AGTCTGACGA CTACGGCAAA GACCTCACCA GCGTAAACAT CTTACTGAAA 
AAGCAGCAGA TGCTGGAGAA TCAGATGGAT GTGCGGAAGA AGGAGATTGA GGAACTGCAG 
AGCCAAGCCC AGGCCTTGAG CCAAGAAGGA AAAAGCACTG ACGAAGTGGA CAGCAAACGC 
CTCACCGTGC AGACCAAATT CATGGAGCTC CTGGAGCCTC TGACCGAACG GAAGAGCAAC 
CTGTTGGCTT CCAAAGAGAT TCATCAGTTC AACCGGGATG TGGAGGACGA GATTCTGTGG 
GTCGGTGAAA GGATGCCCAT AGCCACCTCC ACGGACCATG GCCACAACCT CCAGACCGTG 
CAGCTGTTGA TTAAGAAGAA CCAGACCCTC CAGAAAGAAA TCCAGGGCCA CCAGCCCCGC 
ATCGACGACA TCTACGAGAG GAGTCAGAAT ATCCTCACCG ACAGCAGCCT GAGCGCCGAA 
GCCATCAGGC AGAGGCTGGC GGACCTGAAG CAGCTGTGGA ACCTGGTCAT AGAGGAGACG 
GAAAAGCGGC ACCGGCGGCT GGAGGAATCC CACAAAGCTC AGCAGTACTA CTTCGATGCG 
GCCGAGGCCG AGGCCTGGAT GAGCGAGCAG GAGCTCTACA TGATGTCGGA AGAGAAAGCC 
AAGGATGAGC AGAGCGCCGT GTCTATGCTG AAGAAGCACC AGATTCTGGA ACAAGCGGTG 
GAGGACTACG CCGAGACCGT GCACCAGCTG TCCAAGACCA GCAGGGCTCT GGTGGCCGAC 
AATCACCCGG AGAGTGAGAG AATCAGCATG AGGCAGTCCA AAGTGGATAA ACTGTACGCA 
GGCCTCAAGG ATTTGGCCGA GGAGAGGAGA GGCAAACTGG ACGAGAGACA CAGGCTATTC 
CAGCTGAACC GGGAGGTGGA CGATCTGGAG CAGTGGATCG CCGAGAGGGA GGTGGTGGCT 
GGCTCCCATG AGCTGGGACA GGACTACGAG CACGTCACAA TGCTCCAAGA ACGATTCCGT 
GAATTTGCCC GCGACACCGG AAACATCGGG CAGGAGCGGG TGGACACGGT CAATCACATG 
GCCGACGACC TCATCAACTC CGGACATTCC GACGCCGCCA CCATCGCCGA GTGGAAGGAC 
GGCCTGAACG AGGCCTGGGC CGACCTCCTG GAGCTGATCG ACACGAGAAC GCAGATCCTG 
GCCGCCTCGT ACGAACTGCA CAAATTTTAC CACGATGCCA AGGAGATCTT CGGGCGCATC 
CAGGACAAAC ACAAGAAGCT GCCCGAGGAG CTCGGGAGGG ATCAGAACAC GGTGGAGACG 
CTCCAGAGGA TGCATACGAC ATTTGAGCAT GACATCCAGG CTTTGGGCAC CCAGGTGCGG 
CAGCTGCAGG AAGATGCCGC CCGGCTCCAG GCCGCCTACG CTGGAGACAA GGCCGACGAC 
ATCCAGAAGC GGGAGAATGA GGTCCTGGAA GCCTGGAAAG CCCTCCTGGA CGCCTGCGAG 
GGCCGCAGGN CCCGGCTGGT GGACACCGGA GACAAGTTCC GCTTCTTCAG CTTGGTGCGC 
GACCTCATGC TCTGGATGGA GGACGTCATC CGGCAGATCG AAGCCCAGGA GAAGCCCCGG 
GATGTGTCGT CTGTGGAATT GCTCATGAAC AACCACCAGG GTATCAAAGC GGAAATTGAC 
GCCCGGAACG ACAGCTTCAC TACCTGCATC GAGCTGGGAA AATCACTGTT GGCAAGGAAA 
CACTATGCGT CGGAGGAGAT CAAGGAGAAG CTGTTGCAGT TGACGGAAAA GAGAAAAGAG 
ATGATTGACA AGTGGGAAGA CCGATGGGAG TGGTTAAGGC TGATCCTGGA GGTGCACCAG 
TTTTCGAGGG ACGCCAGCGT GGCCGAGGCC TGGCTTCTGG GCCAGGAGCC CTACCTGTCC 
AGCCGCGAGA TCGGCCAGAG CGTGGACGAG GTGGAGAAGC TCATCAAGCG GCACGAGGCC 
TTCGAGAAAT CGGCTGCCAC GTGGGACGAA CGGTTCTCGG CGCTGGAGAG GCTGACGACG 
TTGGAATTAC TGGAAGTGCG CAGACAGCAA GAGGAAGAGG AGAGAAAGAG GCAGCCTCCT 
TCTCCGGAGC CGAGTACAAA GGTGTCAGAG GAAGCCGAAT CCCAGCAGCA GTGGGATGCT 
TCGAAAGGAG ACCAAGTTTC CCAAAACGGT TTGCCTGCCG AGCAGGGATC TCCACGGCTG 
GCGGAAACTG CCGAAACGAG TGAGATGGTC AACGGAGCCA CCGAGCAGAG GACGAGTTCC 
AAAGAATCCA GTCCGGTTCC TTCCCCGACT TCGGATCGTA AAGCCAAGAC CGTCCTCCAG 
GCCCAGACGG CCGCCACCTT GCCCGCCAAG ACCCAGGAGG TCCCGGCGGC CCAGATGGAG 
GGCTTCTTGA ATCGCAAGCA CGAGTGGGAG GCCCACAATA AGAAAGCCTC CAGCAGGTCT 
TGGCACAACG TATACTGTGT CATCAACAAC CAAGAGATGG GCTTCTACAA GGATGCGAAG 
GCCGCTTCTT CTGGGATACC GTACCACAAC GAGATTCCAG TTAGTTTGAA AGAAGCAGTG 
TGTGAAGTAG CCCTTGACTA CAAAAAGAAA AAGCACGTGT TTAAACTAAG ACTAAATGAC 
GGCAATGAAT ACCTTTTCCA AGCCAAAGAC GATGAGGAAA TGTCCACGTG GATCCAGGCC 
ATCACGTCCG CCATCTCCTC AGACAAGATG GACGTGTCCG CCAGCACCCA GAGCACTCCA 
GCCTCCAGCC GGGCCCAGAC GCTGCCCGCC AGCGTCACAA TAACCAGCGA GTCCAGTCCC 
GGCAAGCGGG AGAAGGACAA GGAAAAGGAC AAAGAGAAAA GGTTCAGCCT CTTTGGCAAA 
AAGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SPTBN1 cDNA ORF clone, Ornithorhynchus anatinus(platypus)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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