LOC110521990 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC110521990
Entrez Gene ID	110521990
Full Name	C-1-tetrahydrofolate synthase, cytoplasmic-like
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021600024.1	XP_021455699.1	C-1-tetrahydrofolate synthase, cytoplasmic-like
XM_021600025.1	XP_021455700.1	C-1-tetrahydrofolate synthase, cytoplasmic-like
XM_021600023.1	XP_021455698.1	C-1-tetrahydrofolate synthase, cytoplasmic-like

Pathways from BioSystems
Gene Ontology

The following LOC110521990 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC110521990 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOf42555	XM_021600025.1 Latest version!	Oncorhynchus mykiss C-1-tetrahydrofolate synthase, cytoplasmic-like (LOC110521990), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OOf42555	XM_021600023.1 Latest version!	Oncorhynchus mykiss C-1-tetrahydrofolate synthase, cytoplasmic-like (LOC110521990), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OOf42555	XM_021600024.1 Latest version!	Oncorhynchus mykiss C-1-tetrahydrofolate synthase, cytoplasmic-like (LOC110521990), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC110521990 ( XM_021600024.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021455699.1 Oncorhynchus mykiss C-1-tetrahydrofolate synthase, cytoplasmic-like (LOC110521990), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOf42555
Clone ID Related Accession (Same CDS sequence)	XM_021600024.1 , XM_021600025.1 , XM_021600023.1
Accession Version	XM_021600024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	C-1-tetrahydrofolate synthase, cytoplasmic-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035080.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTCCT TCGCCGCTCT CACCCTCCGT CTAGGGTCCA CAGGATGCAG AGGAGGTAGG 
CGCAACCTGG CGACAATTGT CTCGGGCAAC AAGACGTCTC AGCTGGTGAG AGAAAGGTTG 
AAAGAGGACT TGGACAAGAT GAGGGGCCAG TTCCCTGGCT TCCGGCCAGG TCTGGTAGTG 
TTGCAGGTGG GTGACAGAGA CGATTCTAAC CTCTACATTA GCATGAAGCT AAAAGCCGCA 
GCTGAGATTG GAATCAATGC CAGCCATGTC AGACTGCCAA AGACTGCAAC AGAGGATGAG 
GTCCTCCGTA GAATTGTAGA GGTCAATGAG AACCTAGAGG TCCACGGCCT CATTGTCCAG 
CTGCCACTGG ACTCTATCAA CCCCATGGAC ACAGAGAAGG TGACTAATGC CGTGGCCCCA 
GAAAAGGATG TGGATGGGCT GACCAGTATC AACGCGGGGA AGCTCTCTCG GGGGGACCTG 
GGGGACTGTT TCATCCCCTG TACTCCTAAA GGCTGCATGA AGCTCATTAG TCAGACAGGC 
ACGTCAGTGG CAGGGAAGAA CGCTGTGGTG ATTGGCCGTA GCAAGATTGT GGGAGCGCCC 
ATGCACGACC TGCTTCTATG GAGCCATGCC ACCGTGACGA CCTGTCATTC CAAAACAACT 
GACCTGGCTG CACAAGTGGG AAGGGCAGAC ATATTGGTGG TGGGTGCAGG CAAGGCGGAG 
ATGGTGAAAG GCGAGTGGTT GAAGGAGGGT GCTGTGGTTA TCGACTGTGG CATCAACCAC 
ATCCCAGATG ACAGCAAAGC CAACGGGATG CGAGTGGTGG GGGATGTGCA TTACCCCTCT 
GCCAAGGAGA AGGCTGGGTT TATTACCCCT GTGCCCGGGG GAGTGGGACC AATGACCGTT 
GCTATGCTCA TGGAGAACAC TGTCCAGAGT GCAAAGCGCT TTCTCAAGAC GTACCAGCCA 
GGAAAGTGGA ACATTTCCTA CGCTAAGCTA AAGCCACAGA AACCTCAACC CAGGGACGCC 
GCGATCGCAC ACTCCTTCAC GCCAAAGTCA ATTGGCCGCC TGGCCCGGGA GGTTGGCCTC 
TTCTCAGAGG AGGTGGAGCC CTACGGGACG ACCCGAGCCA AAGTTCGTCT GGAGGCCTTG 
AACCGCCTCA AGACACAGCC TAATGGGAAA TATTTGGTGG TCACAGGGAT CACACCCACA 
CCACTGGGTG AGGGAACTAC AACCACCACC CTGGGGTTGG CACAGGCCCT AGGGGCACAC 
CTTCATGTCA ACAGTTTTGC CTGTGTGCGC CAACCCAGCC GGGGCTCAAA CTTTGGGGTC 
AAAGGTGGTG CTGTTGGAGG TGGTTATTGC CAAGTCATTC CTATGGAAGA GGTCAGTCTC 
CACCTCACCA GTGACACACA AGCTGTCATG GCAGCCAGTA GCCTGGTGGT GGACACTATC 
AATGCACGTG TGTTATGCGA GTCAACACAG TCAGATAAGG CTCTCTTTGA ATGGCTGGTG 
CCACTGAGGG ACGGTCACAG GAAGTTCTCC CTGTCCCAAC TCAATAGGTT GAAGAGACTG 
GGTATAGAGA AACCAGAAAC CCTGAAACCA GAGGAGATAT ACCGATTTAT CCGTTTGGAC 
ATTGATCCAG AGACCAAGAC GCTGAGTGGA TATGTGGCCA GTGAGATGAT GGCGGTGTTG 
GCCCTCAGTA CCAGTCTGGC AGACATGACG CGCCGCTTGG CCAGGATGGT GGTGGCCTAC 
AGCCGCAAGG GGAAGCCTGT CACAACAGAG GACCTGGGTA TTAGTGGAGT GCTGGCCACA 
TTGATGAGAG ATGCTGTGAA GCCCAGTCTA ATGCAGACCA TGGAGGGGAC ACCTGTGTTT 
GTTCACACCT GTCCATTGTC AGATATTGCC CAGGGCAACT CGTCCATCCT GGCTGACCAG 
ATCGCCTTAA AGCTGGTGGG CCCAGAGGGG TTTGTGGTCA CCGAGGCAGA AGGAGGTGCT 
GAATTAGGCA TGGAGAAGTT CTTCGACATC AAGTGTCGCT CTTCGGGCCT GCATCCTGAC 
GTGGTGGTGA TGGTGGCTTC AGTTCCTGCC CTGAAGATGC ATGGGGGTGG ACCTGCGGTC 
ACGGCAGGTT CAGCCATGCC GAAGGAGTAC TCAGCGGAGA ACCTGACGTT ACTGGAAAAT 
GGCTGCAACC ACTTGAAGAG GCAGCTGGAG AATGCCCGGG CTTTTGGCTT ACCTGTCGTT 
GTAGCCATCA ACACCTTCAG CACCGACACT GATGCAGAGT TGGGCCTAGT CTGTGAGCAG 
GCTAAACTGG CGGGGGCCTT GGAGGCGGTG CCATGCTCAC ACTGGGCTGA AGGGGGTGCC 
GGAGCGGTGG CGTTGGGTCA GGCGGTACAG AGGGCAGCTG AGACACCACA CCAGATTAAC 
TTCCTGTATG ACCTGGAGAT GCCCATTGAC GACAAAATCA GAGTAATAGC AAAGTCAATG 
TATGGAGCGG ATGATGTGGA GCTCCTTCCT CAGGCCCAAC AGAAGGTGGC GCTGTTCTCA 
AAACAAGGCT TGGGCAACCT GCCCATATGC ATGGCAAAGA CCCACCTGTC CCTGTCCCAC 
GACCCAGAGA GGAAAGGGGT GCCCACCGGC TTTACCTTGC CCATCAGGGA CATCCATGCC 
AATTTGGGTG CTGGTTTTCT CTACCCCTTG GTTGGCACGT CGAGTGTAGC ACCCCAATCT 
CCAGCCTTCT CCTGTTTCCA TGACAACGAC TTCAGCACTG AGAGCAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021455699.1
CDS	1609..4359
Translation	MLSFAALTLRLGSTGCRGGRRNLATIVSGNKTSQLVRERLKEDLDKMRGQFPGFRPGLVVLQVGDRDDSNLYISMKLKAAAEIGINASHVRLPKTATEDEVLRRIVEVNENLEVHGLIVQLPLDSINPMDTEKVTNAVAPEKDVDGLTSINAGKLSRGDLGDCFIPCTPKGCMKLISQTGTSVAGKNAVVIGRSKIVGAPMHDLLLWSHATVTTCHSKTTDLAAQVGRADILVVGAGKAEMVKGEWLKEGAVVIDCGINHIPDDSKANGMRVVGDVHYPSAKEKAGFITPVPGGVGPMTVAMLMENTVQSAKRFLKTYQPGKWNISYAKLKPQKPQPRDAAIAHSFTPKSIGRLAREVGLFSEEVEPYGTTRAKVRLEALNRLKTQPNGKYLVVTGITPTPLGEGTTTTTLGLAQALGAHLHVNSFACVRQPSRGSNFGVKGGAVGGGYCQVIPMEEVSLHLTSDTQAVMAASSLVVDTINARVLCESTQSDKALFEWLVPLRDGHRKFSLSQLNRLKRLGIEKPETLKPEEIYRFIRLDIDPETKTLSGYVASEMMAVLALSTSLADMTRRLARMVVAYSRKGKPVTTEDLGISGVLATLMRDAVKPSLMQTMEGTPVFVHTCPLSDIAQGNSSILADQIALKLVGPEGFVVTEAEGGAELGMEKFFDIKCRSSGLHPDVVVMVASVPALKMHGGGPAVTAGSAMPKEYSAENLTLLENGCNHLKRQLENARAFGLPVVVAINTFSTDTDAELGLVCEQAKLAGALEAVPCSHWAEGGAGAVALGQAVQRAAETPHQINFLYDLEMPIDDKIRVIAKSMYGADDVELLPQAQQKVALFSKQGLGNLPICMAKTHLSLSHDPERKGVPTGFTLPIRDIHANLGAGFLYPLVGTSSVAPQSPAFSCFHDNDFSTESK

Target ORF information:

RefSeq Version	XM_021600024.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss C-1-tetrahydrofolate synthase, cytoplasmic-like (LOC110521990), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021600024.1

ORF Insert Sequence:

ATGCTTTCCT TCGCCGCTCT CACCCTCCGT CTAGGGTCCA CAGGATGCAG AGGAGGTAGG 
CGCAACCTGG CGACAATTGT CTCGGGCAAC AAGACGTCTC AGCTGGTGAG AGAAAGGTTG 
AAAGAGGACT TGGACAAGAT GAGGGGCCAG TTCCCTGGCT TCCGGCCAGG TCTGGTAGTG 
TTGCAGGTGG GTGACAGAGA CGATTCTAAC CTCTACATTA GCATGAAGCT AAAAGCCGCA 
GCTGAGATTG GAATCAATGC CAGCCATGTC AGACTGCCAA AGACTGCAAC AGAGGATGAG 
GTCCTCCGTA GAATTGTAGA GGTCAATGAG AACCTAGAGG TCCACGGCCT CATTGTCCAG 
CTGCCACTGG ACTCTATCAA CCCCATGGAC ACAGAGAAGG TGACTAATGC CGTGGCCCCA 
GAAAAGGATG TGGATGGGCT GACCAGTATC AACGCGGGGA AGCTCTCTCG GGGGGACCTG 
GGGGACTGTT TCATCCCCTG TACTCCTAAA GGCTGCATGA AGCTCATTAG TCAGACAGGC 
ACGTCAGTGG CAGGGAAGAA CGCTGTGGTG ATTGGCCGTA GCAAGATTGT GGGAGCGCCC 
ATGCACGACC TGCTTCTATG GAGCCATGCC ACCGTGACGA CCTGTCATTC CAAAACAACT 
GACCTGGCTG CACAAGTGGG AAGGGCAGAC ATATTGGTGG TGGGTGCAGG CAAGGCGGAG 
ATGGTGAAAG GCGAGTGGTT GAAGGAGGGT GCTGTGGTTA TCGACTGTGG CATCAACCAC 
ATCCCAGATG ACAGCAAAGC CAACGGGATG CGAGTGGTGG GGGATGTGCA TTACCCCTCT 
GCCAAGGAGA AGGCTGGGTT TATTACCCCT GTGCCCGGGG GAGTGGGACC AATGACCGTT 
GCTATGCTCA TGGAGAACAC TGTCCAGAGT GCAAAGCGCT TTCTCAAGAC GTACCAGCCA 
GGAAAGTGGA ACATTTCCTA CGCTAAGCTA AAGCCACAGA AACCTCAACC CAGGGACGCC 
GCGATCGCAC ACTCCTTCAC GCCAAAGTCA ATTGGCCGCC TGGCCCGGGA GGTTGGCCTC 
TTCTCAGAGG AGGTGGAGCC CTACGGGACG ACCCGAGCCA AAGTTCGTCT GGAGGCCTTG 
AACCGCCTCA AGACACAGCC TAATGGGAAA TATTTGGTGG TCACAGGGAT CACACCCACA 
CCACTGGGTG AGGGAACTAC AACCACCACC CTGGGGTTGG CACAGGCCCT AGGGGCACAC 
CTTCATGTCA ACAGTTTTGC CTGTGTGCGC CAACCCAGCC GGGGCTCAAA CTTTGGGGTC 
AAAGGTGGTG CTGTTGGAGG TGGTTATTGC CAAGTCATTC CTATGGAAGA GGTCAGTCTC 
CACCTCACCA GTGACACACA AGCTGTCATG GCAGCCAGTA GCCTGGTGGT GGACACTATC 
AATGCACGTG TGTTATGCGA GTCAACACAG TCAGATAAGG CTCTCTTTGA ATGGCTGGTG 
CCACTGAGGG ACGGTCACAG GAAGTTCTCC CTGTCCCAAC TCAATAGGTT GAAGAGACTG 
GGTATAGAGA AACCAGAAAC CCTGAAACCA GAGGAGATAT ACCGATTTAT CCGTTTGGAC 
ATTGATCCAG AGACCAAGAC GCTGAGTGGA TATGTGGCCA GTGAGATGAT GGCGGTGTTG 
GCCCTCAGTA CCAGTCTGGC AGACATGACG CGCCGCTTGG CCAGGATGGT GGTGGCCTAC 
AGCCGCAAGG GGAAGCCTGT CACAACAGAG GACCTGGGTA TTAGTGGAGT GCTGGCCACA 
TTGATGAGAG ATGCTGTGAA GCCCAGTCTA ATGCAGACCA TGGAGGGGAC ACCTGTGTTT 
GTTCACACCT GTCCATTGTC AGATATTGCC CAGGGCAACT CGTCCATCCT GGCTGACCAG 
ATCGCCTTAA AGCTGGTGGG CCCAGAGGGG TTTGTGGTCA CCGAGGCAGA AGGAGGTGCT 
GAATTAGGCA TGGAGAAGTT CTTCGACATC AAGTGTCGCT CTTCGGGCCT GCATCCTGAC 
GTGGTGGTGA TGGTGGCTTC AGTTCCTGCC CTGAAGATGC ATGGGGGTGG ACCTGCGGTC 
ACGGCAGGTT CAGCCATGCC GAAGGAGTAC TCAGCGGAGA ACCTGACGTT ACTGGAAAAT 
GGCTGCAACC ACTTGAAGAG GCAGCTGGAG AATGCCCGGG CTTTTGGCTT ACCTGTCGTT 
GTAGCCATCA ACACCTTCAG CACCGACACT GATGCAGAGT TGGGCCTAGT CTGTGAGCAG 
GCTAAACTGG CGGGGGCCTT GGAGGCGGTG CCATGCTCAC ACTGGGCTGA AGGGGGTGCC 
GGAGCGGTGG CGTTGGGTCA GGCGGTACAG AGGGCAGCTG AGACACCACA CCAGATTAAC 
TTCCTGTATG ACCTGGAGAT GCCCATTGAC GACAAAATCA GAGTAATAGC AAAGTCAATG 
TATGGAGCGG ATGATGTGGA GCTCCTTCCT CAGGCCCAAC AGAAGGTGGC GCTGTTCTCA 
AAACAAGGCT TGGGCAACCT GCCCATATGC ATGGCAAAGA CCCACCTGTC CCTGTCCCAC 
GACCCAGAGA GGAAAGGGGT GCCCACCGGC TTTACCTTGC CCATCAGGGA CATCCATGCC 
AATTTGGGTG CTGGTTTTCT CTACCCCTTG GTTGGCACGT CGAGTGTAGC ACCCCAATCT 
CCAGCCTTCT CCTGTTTCCA TGACAACGAC TTCAGCACTG AGAGCAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110521990 ( XM_021600025.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021455700.1 Oncorhynchus mykiss C-1-tetrahydrofolate synthase, cytoplasmic-like (LOC110521990), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOf42555
Clone ID Related Accession (Same CDS sequence)	XM_021600024.1 , XM_021600025.1 , XM_021600023.1
Accession Version	XM_021600025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	C-1-tetrahydrofolate synthase, cytoplasmic-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035080.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTCCT TCGCCGCTCT CACCCTCCGT CTAGGGTCCA CAGGATGCAG AGGAGGTAGG 
CGCAACCTGG CGACAATTGT CTCGGGCAAC AAGACGTCTC AGCTGGTGAG AGAAAGGTTG 
AAAGAGGACT TGGACAAGAT GAGGGGCCAG TTCCCTGGCT TCCGGCCAGG TCTGGTAGTG 
TTGCAGGTGG GTGACAGAGA CGATTCTAAC CTCTACATTA GCATGAAGCT AAAAGCCGCA 
GCTGAGATTG GAATCAATGC CAGCCATGTC AGACTGCCAA AGACTGCAAC AGAGGATGAG 
GTCCTCCGTA GAATTGTAGA GGTCAATGAG AACCTAGAGG TCCACGGCCT CATTGTCCAG 
CTGCCACTGG ACTCTATCAA CCCCATGGAC ACAGAGAAGG TGACTAATGC CGTGGCCCCA 
GAAAAGGATG TGGATGGGCT GACCAGTATC AACGCGGGGA AGCTCTCTCG GGGGGACCTG 
GGGGACTGTT TCATCCCCTG TACTCCTAAA GGCTGCATGA AGCTCATTAG TCAGACAGGC 
ACGTCAGTGG CAGGGAAGAA CGCTGTGGTG ATTGGCCGTA GCAAGATTGT GGGAGCGCCC 
ATGCACGACC TGCTTCTATG GAGCCATGCC ACCGTGACGA CCTGTCATTC CAAAACAACT 
GACCTGGCTG CACAAGTGGG AAGGGCAGAC ATATTGGTGG TGGGTGCAGG CAAGGCGGAG 
ATGGTGAAAG GCGAGTGGTT GAAGGAGGGT GCTGTGGTTA TCGACTGTGG CATCAACCAC 
ATCCCAGATG ACAGCAAAGC CAACGGGATG CGAGTGGTGG GGGATGTGCA TTACCCCTCT 
GCCAAGGAGA AGGCTGGGTT TATTACCCCT GTGCCCGGGG GAGTGGGACC AATGACCGTT 
GCTATGCTCA TGGAGAACAC TGTCCAGAGT GCAAAGCGCT TTCTCAAGAC GTACCAGCCA 
GGAAAGTGGA ACATTTCCTA CGCTAAGCTA AAGCCACAGA AACCTCAACC CAGGGACGCC 
GCGATCGCAC ACTCCTTCAC GCCAAAGTCA ATTGGCCGCC TGGCCCGGGA GGTTGGCCTC 
TTCTCAGAGG AGGTGGAGCC CTACGGGACG ACCCGAGCCA AAGTTCGTCT GGAGGCCTTG 
AACCGCCTCA AGACACAGCC TAATGGGAAA TATTTGGTGG TCACAGGGAT CACACCCACA 
CCACTGGGTG AGGGAACTAC AACCACCACC CTGGGGTTGG CACAGGCCCT AGGGGCACAC 
CTTCATGTCA ACAGTTTTGC CTGTGTGCGC CAACCCAGCC GGGGCTCAAA CTTTGGGGTC 
AAAGGTGGTG CTGTTGGAGG TGGTTATTGC CAAGTCATTC CTATGGAAGA GGTCAGTCTC 
CACCTCACCA GTGACACACA AGCTGTCATG GCAGCCAGTA GCCTGGTGGT GGACACTATC 
AATGCACGTG TGTTATGCGA GTCAACACAG TCAGATAAGG CTCTCTTTGA ATGGCTGGTG 
CCACTGAGGG ACGGTCACAG GAAGTTCTCC CTGTCCCAAC TCAATAGGTT GAAGAGACTG 
GGTATAGAGA AACCAGAAAC CCTGAAACCA GAGGAGATAT ACCGATTTAT CCGTTTGGAC 
ATTGATCCAG AGACCAAGAC GCTGAGTGGA TATGTGGCCA GTGAGATGAT GGCGGTGTTG 
GCCCTCAGTA CCAGTCTGGC AGACATGACG CGCCGCTTGG CCAGGATGGT GGTGGCCTAC 
AGCCGCAAGG GGAAGCCTGT CACAACAGAG GACCTGGGTA TTAGTGGAGT GCTGGCCACA 
TTGATGAGAG ATGCTGTGAA GCCCAGTCTA ATGCAGACCA TGGAGGGGAC ACCTGTGTTT 
GTTCACACCT GTCCATTGTC AGATATTGCC CAGGGCAACT CGTCCATCCT GGCTGACCAG 
ATCGCCTTAA AGCTGGTGGG CCCAGAGGGG TTTGTGGTCA CCGAGGCAGA AGGAGGTGCT 
GAATTAGGCA TGGAGAAGTT CTTCGACATC AAGTGTCGCT CTTCGGGCCT GCATCCTGAC 
GTGGTGGTGA TGGTGGCTTC AGTTCCTGCC CTGAAGATGC ATGGGGGTGG ACCTGCGGTC 
ACGGCAGGTT CAGCCATGCC GAAGGAGTAC TCAGCGGAGA ACCTGACGTT ACTGGAAAAT 
GGCTGCAACC ACTTGAAGAG GCAGCTGGAG AATGCCCGGG CTTTTGGCTT ACCTGTCGTT 
GTAGCCATCA ACACCTTCAG CACCGACACT GATGCAGAGT TGGGCCTAGT CTGTGAGCAG 
GCTAAACTGG CGGGGGCCTT GGAGGCGGTG CCATGCTCAC ACTGGGCTGA AGGGGGTGCC 
GGAGCGGTGG CGTTGGGTCA GGCGGTACAG AGGGCAGCTG AGACACCACA CCAGATTAAC 
TTCCTGTATG ACCTGGAGAT GCCCATTGAC GACAAAATCA GAGTAATAGC AAAGTCAATG 
TATGGAGCGG ATGATGTGGA GCTCCTTCCT CAGGCCCAAC AGAAGGTGGC GCTGTTCTCA 
AAACAAGGCT TGGGCAACCT GCCCATATGC ATGGCAAAGA CCCACCTGTC CCTGTCCCAC 
GACCCAGAGA GGAAAGGGGT GCCCACCGGC TTTACCTTGC CCATCAGGGA CATCCATGCC 
AATTTGGGTG CTGGTTTTCT CTACCCCTTG GTTGGCACGT CGAGTGTAGC ACCCCAATCT 
CCAGCCTTCT CCTGTTTCCA TGACAACGAC TTCAGCACTG AGAGCAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021455700.1
CDS	1959..4709
Translation	MLSFAALTLRLGSTGCRGGRRNLATIVSGNKTSQLVRERLKEDLDKMRGQFPGFRPGLVVLQVGDRDDSNLYISMKLKAAAEIGINASHVRLPKTATEDEVLRRIVEVNENLEVHGLIVQLPLDSINPMDTEKVTNAVAPEKDVDGLTSINAGKLSRGDLGDCFIPCTPKGCMKLISQTGTSVAGKNAVVIGRSKIVGAPMHDLLLWSHATVTTCHSKTTDLAAQVGRADILVVGAGKAEMVKGEWLKEGAVVIDCGINHIPDDSKANGMRVVGDVHYPSAKEKAGFITPVPGGVGPMTVAMLMENTVQSAKRFLKTYQPGKWNISYAKLKPQKPQPRDAAIAHSFTPKSIGRLAREVGLFSEEVEPYGTTRAKVRLEALNRLKTQPNGKYLVVTGITPTPLGEGTTTTTLGLAQALGAHLHVNSFACVRQPSRGSNFGVKGGAVGGGYCQVIPMEEVSLHLTSDTQAVMAASSLVVDTINARVLCESTQSDKALFEWLVPLRDGHRKFSLSQLNRLKRLGIEKPETLKPEEIYRFIRLDIDPETKTLSGYVASEMMAVLALSTSLADMTRRLARMVVAYSRKGKPVTTEDLGISGVLATLMRDAVKPSLMQTMEGTPVFVHTCPLSDIAQGNSSILADQIALKLVGPEGFVVTEAEGGAELGMEKFFDIKCRSSGLHPDVVVMVASVPALKMHGGGPAVTAGSAMPKEYSAENLTLLENGCNHLKRQLENARAFGLPVVVAINTFSTDTDAELGLVCEQAKLAGALEAVPCSHWAEGGAGAVALGQAVQRAAETPHQINFLYDLEMPIDDKIRVIAKSMYGADDVELLPQAQQKVALFSKQGLGNLPICMAKTHLSLSHDPERKGVPTGFTLPIRDIHANLGAGFLYPLVGTSSVAPQSPAFSCFHDNDFSTESK

Target ORF information:

RefSeq Version	XM_021600025.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss C-1-tetrahydrofolate synthase, cytoplasmic-like (LOC110521990), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021600025.1

ORF Insert Sequence:

ATGCTTTCCT TCGCCGCTCT CACCCTCCGT CTAGGGTCCA CAGGATGCAG AGGAGGTAGG 
CGCAACCTGG CGACAATTGT CTCGGGCAAC AAGACGTCTC AGCTGGTGAG AGAAAGGTTG 
AAAGAGGACT TGGACAAGAT GAGGGGCCAG TTCCCTGGCT TCCGGCCAGG TCTGGTAGTG 
TTGCAGGTGG GTGACAGAGA CGATTCTAAC CTCTACATTA GCATGAAGCT AAAAGCCGCA 
GCTGAGATTG GAATCAATGC CAGCCATGTC AGACTGCCAA AGACTGCAAC AGAGGATGAG 
GTCCTCCGTA GAATTGTAGA GGTCAATGAG AACCTAGAGG TCCACGGCCT CATTGTCCAG 
CTGCCACTGG ACTCTATCAA CCCCATGGAC ACAGAGAAGG TGACTAATGC CGTGGCCCCA 
GAAAAGGATG TGGATGGGCT GACCAGTATC AACGCGGGGA AGCTCTCTCG GGGGGACCTG 
GGGGACTGTT TCATCCCCTG TACTCCTAAA GGCTGCATGA AGCTCATTAG TCAGACAGGC 
ACGTCAGTGG CAGGGAAGAA CGCTGTGGTG ATTGGCCGTA GCAAGATTGT GGGAGCGCCC 
ATGCACGACC TGCTTCTATG GAGCCATGCC ACCGTGACGA CCTGTCATTC CAAAACAACT 
GACCTGGCTG CACAAGTGGG AAGGGCAGAC ATATTGGTGG TGGGTGCAGG CAAGGCGGAG 
ATGGTGAAAG GCGAGTGGTT GAAGGAGGGT GCTGTGGTTA TCGACTGTGG CATCAACCAC 
ATCCCAGATG ACAGCAAAGC CAACGGGATG CGAGTGGTGG GGGATGTGCA TTACCCCTCT 
GCCAAGGAGA AGGCTGGGTT TATTACCCCT GTGCCCGGGG GAGTGGGACC AATGACCGTT 
GCTATGCTCA TGGAGAACAC TGTCCAGAGT GCAAAGCGCT TTCTCAAGAC GTACCAGCCA 
GGAAAGTGGA ACATTTCCTA CGCTAAGCTA AAGCCACAGA AACCTCAACC CAGGGACGCC 
GCGATCGCAC ACTCCTTCAC GCCAAAGTCA ATTGGCCGCC TGGCCCGGGA GGTTGGCCTC 
TTCTCAGAGG AGGTGGAGCC CTACGGGACG ACCCGAGCCA AAGTTCGTCT GGAGGCCTTG 
AACCGCCTCA AGACACAGCC TAATGGGAAA TATTTGGTGG TCACAGGGAT CACACCCACA 
CCACTGGGTG AGGGAACTAC AACCACCACC CTGGGGTTGG CACAGGCCCT AGGGGCACAC 
CTTCATGTCA ACAGTTTTGC CTGTGTGCGC CAACCCAGCC GGGGCTCAAA CTTTGGGGTC 
AAAGGTGGTG CTGTTGGAGG TGGTTATTGC CAAGTCATTC CTATGGAAGA GGTCAGTCTC 
CACCTCACCA GTGACACACA AGCTGTCATG GCAGCCAGTA GCCTGGTGGT GGACACTATC 
AATGCACGTG TGTTATGCGA GTCAACACAG TCAGATAAGG CTCTCTTTGA ATGGCTGGTG 
CCACTGAGGG ACGGTCACAG GAAGTTCTCC CTGTCCCAAC TCAATAGGTT GAAGAGACTG 
GGTATAGAGA AACCAGAAAC CCTGAAACCA GAGGAGATAT ACCGATTTAT CCGTTTGGAC 
ATTGATCCAG AGACCAAGAC GCTGAGTGGA TATGTGGCCA GTGAGATGAT GGCGGTGTTG 
GCCCTCAGTA CCAGTCTGGC AGACATGACG CGCCGCTTGG CCAGGATGGT GGTGGCCTAC 
AGCCGCAAGG GGAAGCCTGT CACAACAGAG GACCTGGGTA TTAGTGGAGT GCTGGCCACA 
TTGATGAGAG ATGCTGTGAA GCCCAGTCTA ATGCAGACCA TGGAGGGGAC ACCTGTGTTT 
GTTCACACCT GTCCATTGTC AGATATTGCC CAGGGCAACT CGTCCATCCT GGCTGACCAG 
ATCGCCTTAA AGCTGGTGGG CCCAGAGGGG TTTGTGGTCA CCGAGGCAGA AGGAGGTGCT 
GAATTAGGCA TGGAGAAGTT CTTCGACATC AAGTGTCGCT CTTCGGGCCT GCATCCTGAC 
GTGGTGGTGA TGGTGGCTTC AGTTCCTGCC CTGAAGATGC ATGGGGGTGG ACCTGCGGTC 
ACGGCAGGTT CAGCCATGCC GAAGGAGTAC TCAGCGGAGA ACCTGACGTT ACTGGAAAAT 
GGCTGCAACC ACTTGAAGAG GCAGCTGGAG AATGCCCGGG CTTTTGGCTT ACCTGTCGTT 
GTAGCCATCA ACACCTTCAG CACCGACACT GATGCAGAGT TGGGCCTAGT CTGTGAGCAG 
GCTAAACTGG CGGGGGCCTT GGAGGCGGTG CCATGCTCAC ACTGGGCTGA AGGGGGTGCC 
GGAGCGGTGG CGTTGGGTCA GGCGGTACAG AGGGCAGCTG AGACACCACA CCAGATTAAC 
TTCCTGTATG ACCTGGAGAT GCCCATTGAC GACAAAATCA GAGTAATAGC AAAGTCAATG 
TATGGAGCGG ATGATGTGGA GCTCCTTCCT CAGGCCCAAC AGAAGGTGGC GCTGTTCTCA 
AAACAAGGCT TGGGCAACCT GCCCATATGC ATGGCAAAGA CCCACCTGTC CCTGTCCCAC 
GACCCAGAGA GGAAAGGGGT GCCCACCGGC TTTACCTTGC CCATCAGGGA CATCCATGCC 
AATTTGGGTG CTGGTTTTCT CTACCCCTTG GTTGGCACGT CGAGTGTAGC ACCCCAATCT 
CCAGCCTTCT CCTGTTTCCA TGACAACGAC TTCAGCACTG AGAGCAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC110521990 ( XM_021600023.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021455698.1 Oncorhynchus mykiss C-1-tetrahydrofolate synthase, cytoplasmic-like (LOC110521990), transcript variant X1, mRNA.

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CloneID	OOf42555
Clone ID Related Accession (Same CDS sequence)	XM_021600024.1 , XM_021600025.1 , XM_021600023.1
Accession Version	XM_021600023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	C-1-tetrahydrofolate synthase, cytoplasmic-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035080.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTCCT TCGCCGCTCT CACCCTCCGT CTAGGGTCCA CAGGATGCAG AGGAGGTAGG 
CGCAACCTGG CGACAATTGT CTCGGGCAAC AAGACGTCTC AGCTGGTGAG AGAAAGGTTG 
AAAGAGGACT TGGACAAGAT GAGGGGCCAG TTCCCTGGCT TCCGGCCAGG TCTGGTAGTG 
TTGCAGGTGG GTGACAGAGA CGATTCTAAC CTCTACATTA GCATGAAGCT AAAAGCCGCA 
GCTGAGATTG GAATCAATGC CAGCCATGTC AGACTGCCAA AGACTGCAAC AGAGGATGAG 
GTCCTCCGTA GAATTGTAGA GGTCAATGAG AACCTAGAGG TCCACGGCCT CATTGTCCAG 
CTGCCACTGG ACTCTATCAA CCCCATGGAC ACAGAGAAGG TGACTAATGC CGTGGCCCCA 
GAAAAGGATG TGGATGGGCT GACCAGTATC AACGCGGGGA AGCTCTCTCG GGGGGACCTG 
GGGGACTGTT TCATCCCCTG TACTCCTAAA GGCTGCATGA AGCTCATTAG TCAGACAGGC 
ACGTCAGTGG CAGGGAAGAA CGCTGTGGTG ATTGGCCGTA GCAAGATTGT GGGAGCGCCC 
ATGCACGACC TGCTTCTATG GAGCCATGCC ACCGTGACGA CCTGTCATTC CAAAACAACT 
GACCTGGCTG CACAAGTGGG AAGGGCAGAC ATATTGGTGG TGGGTGCAGG CAAGGCGGAG 
ATGGTGAAAG GCGAGTGGTT GAAGGAGGGT GCTGTGGTTA TCGACTGTGG CATCAACCAC 
ATCCCAGATG ACAGCAAAGC CAACGGGATG CGAGTGGTGG GGGATGTGCA TTACCCCTCT 
GCCAAGGAGA AGGCTGGGTT TATTACCCCT GTGCCCGGGG GAGTGGGACC AATGACCGTT 
GCTATGCTCA TGGAGAACAC TGTCCAGAGT GCAAAGCGCT TTCTCAAGAC GTACCAGCCA 
GGAAAGTGGA ACATTTCCTA CGCTAAGCTA AAGCCACAGA AACCTCAACC CAGGGACGCC 
GCGATCGCAC ACTCCTTCAC GCCAAAGTCA ATTGGCCGCC TGGCCCGGGA GGTTGGCCTC 
TTCTCAGAGG AGGTGGAGCC CTACGGGACG ACCCGAGCCA AAGTTCGTCT GGAGGCCTTG 
AACCGCCTCA AGACACAGCC TAATGGGAAA TATTTGGTGG TCACAGGGAT CACACCCACA 
CCACTGGGTG AGGGAACTAC AACCACCACC CTGGGGTTGG CACAGGCCCT AGGGGCACAC 
CTTCATGTCA ACAGTTTTGC CTGTGTGCGC CAACCCAGCC GGGGCTCAAA CTTTGGGGTC 
AAAGGTGGTG CTGTTGGAGG TGGTTATTGC CAAGTCATTC CTATGGAAGA GGTCAGTCTC 
CACCTCACCA GTGACACACA AGCTGTCATG GCAGCCAGTA GCCTGGTGGT GGACACTATC 
AATGCACGTG TGTTATGCGA GTCAACACAG TCAGATAAGG CTCTCTTTGA ATGGCTGGTG 
CCACTGAGGG ACGGTCACAG GAAGTTCTCC CTGTCCCAAC TCAATAGGTT GAAGAGACTG 
GGTATAGAGA AACCAGAAAC CCTGAAACCA GAGGAGATAT ACCGATTTAT CCGTTTGGAC 
ATTGATCCAG AGACCAAGAC GCTGAGTGGA TATGTGGCCA GTGAGATGAT GGCGGTGTTG 
GCCCTCAGTA CCAGTCTGGC AGACATGACG CGCCGCTTGG CCAGGATGGT GGTGGCCTAC 
AGCCGCAAGG GGAAGCCTGT CACAACAGAG GACCTGGGTA TTAGTGGAGT GCTGGCCACA 
TTGATGAGAG ATGCTGTGAA GCCCAGTCTA ATGCAGACCA TGGAGGGGAC ACCTGTGTTT 
GTTCACACCT GTCCATTGTC AGATATTGCC CAGGGCAACT CGTCCATCCT GGCTGACCAG 
ATCGCCTTAA AGCTGGTGGG CCCAGAGGGG TTTGTGGTCA CCGAGGCAGA AGGAGGTGCT 
GAATTAGGCA TGGAGAAGTT CTTCGACATC AAGTGTCGCT CTTCGGGCCT GCATCCTGAC 
GTGGTGGTGA TGGTGGCTTC AGTTCCTGCC CTGAAGATGC ATGGGGGTGG ACCTGCGGTC 
ACGGCAGGTT CAGCCATGCC GAAGGAGTAC TCAGCGGAGA ACCTGACGTT ACTGGAAAAT 
GGCTGCAACC ACTTGAAGAG GCAGCTGGAG AATGCCCGGG CTTTTGGCTT ACCTGTCGTT 
GTAGCCATCA ACACCTTCAG CACCGACACT GATGCAGAGT TGGGCCTAGT CTGTGAGCAG 
GCTAAACTGG CGGGGGCCTT GGAGGCGGTG CCATGCTCAC ACTGGGCTGA AGGGGGTGCC 
GGAGCGGTGG CGTTGGGTCA GGCGGTACAG AGGGCAGCTG AGACACCACA CCAGATTAAC 
TTCCTGTATG ACCTGGAGAT GCCCATTGAC GACAAAATCA GAGTAATAGC AAAGTCAATG 
TATGGAGCGG ATGATGTGGA GCTCCTTCCT CAGGCCCAAC AGAAGGTGGC GCTGTTCTCA 
AAACAAGGCT TGGGCAACCT GCCCATATGC ATGGCAAAGA CCCACCTGTC CCTGTCCCAC 
GACCCAGAGA GGAAAGGGGT GCCCACCGGC TTTACCTTGC CCATCAGGGA CATCCATGCC 
AATTTGGGTG CTGGTTTTCT CTACCCCTTG GTTGGCACGT CGAGTGTAGC ACCCCAATCT 
CCAGCCTTCT CCTGTTTCCA TGACAACGAC TTCAGCACTG AGAGCAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021455698.1
CDS	1957..4707
Translation	MLSFAALTLRLGSTGCRGGRRNLATIVSGNKTSQLVRERLKEDLDKMRGQFPGFRPGLVVLQVGDRDDSNLYISMKLKAAAEIGINASHVRLPKTATEDEVLRRIVEVNENLEVHGLIVQLPLDSINPMDTEKVTNAVAPEKDVDGLTSINAGKLSRGDLGDCFIPCTPKGCMKLISQTGTSVAGKNAVVIGRSKIVGAPMHDLLLWSHATVTTCHSKTTDLAAQVGRADILVVGAGKAEMVKGEWLKEGAVVIDCGINHIPDDSKANGMRVVGDVHYPSAKEKAGFITPVPGGVGPMTVAMLMENTVQSAKRFLKTYQPGKWNISYAKLKPQKPQPRDAAIAHSFTPKSIGRLAREVGLFSEEVEPYGTTRAKVRLEALNRLKTQPNGKYLVVTGITPTPLGEGTTTTTLGLAQALGAHLHVNSFACVRQPSRGSNFGVKGGAVGGGYCQVIPMEEVSLHLTSDTQAVMAASSLVVDTINARVLCESTQSDKALFEWLVPLRDGHRKFSLSQLNRLKRLGIEKPETLKPEEIYRFIRLDIDPETKTLSGYVASEMMAVLALSTSLADMTRRLARMVVAYSRKGKPVTTEDLGISGVLATLMRDAVKPSLMQTMEGTPVFVHTCPLSDIAQGNSSILADQIALKLVGPEGFVVTEAEGGAELGMEKFFDIKCRSSGLHPDVVVMVASVPALKMHGGGPAVTAGSAMPKEYSAENLTLLENGCNHLKRQLENARAFGLPVVVAINTFSTDTDAELGLVCEQAKLAGALEAVPCSHWAEGGAGAVALGQAVQRAAETPHQINFLYDLEMPIDDKIRVIAKSMYGADDVELLPQAQQKVALFSKQGLGNLPICMAKTHLSLSHDPERKGVPTGFTLPIRDIHANLGAGFLYPLVGTSSVAPQSPAFSCFHDNDFSTESK

Target ORF information:

RefSeq Version	XM_021600023.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss C-1-tetrahydrofolate synthase, cytoplasmic-like (LOC110521990), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021600023.1

ORF Insert Sequence:

ATGCTTTCCT TCGCCGCTCT CACCCTCCGT CTAGGGTCCA CAGGATGCAG AGGAGGTAGG 
CGCAACCTGG CGACAATTGT CTCGGGCAAC AAGACGTCTC AGCTGGTGAG AGAAAGGTTG 
AAAGAGGACT TGGACAAGAT GAGGGGCCAG TTCCCTGGCT TCCGGCCAGG TCTGGTAGTG 
TTGCAGGTGG GTGACAGAGA CGATTCTAAC CTCTACATTA GCATGAAGCT AAAAGCCGCA 
GCTGAGATTG GAATCAATGC CAGCCATGTC AGACTGCCAA AGACTGCAAC AGAGGATGAG 
GTCCTCCGTA GAATTGTAGA GGTCAATGAG AACCTAGAGG TCCACGGCCT CATTGTCCAG 
CTGCCACTGG ACTCTATCAA CCCCATGGAC ACAGAGAAGG TGACTAATGC CGTGGCCCCA 
GAAAAGGATG TGGATGGGCT GACCAGTATC AACGCGGGGA AGCTCTCTCG GGGGGACCTG 
GGGGACTGTT TCATCCCCTG TACTCCTAAA GGCTGCATGA AGCTCATTAG TCAGACAGGC 
ACGTCAGTGG CAGGGAAGAA CGCTGTGGTG ATTGGCCGTA GCAAGATTGT GGGAGCGCCC 
ATGCACGACC TGCTTCTATG GAGCCATGCC ACCGTGACGA CCTGTCATTC CAAAACAACT 
GACCTGGCTG CACAAGTGGG AAGGGCAGAC ATATTGGTGG TGGGTGCAGG CAAGGCGGAG 
ATGGTGAAAG GCGAGTGGTT GAAGGAGGGT GCTGTGGTTA TCGACTGTGG CATCAACCAC 
ATCCCAGATG ACAGCAAAGC CAACGGGATG CGAGTGGTGG GGGATGTGCA TTACCCCTCT 
GCCAAGGAGA AGGCTGGGTT TATTACCCCT GTGCCCGGGG GAGTGGGACC AATGACCGTT 
GCTATGCTCA TGGAGAACAC TGTCCAGAGT GCAAAGCGCT TTCTCAAGAC GTACCAGCCA 
GGAAAGTGGA ACATTTCCTA CGCTAAGCTA AAGCCACAGA AACCTCAACC CAGGGACGCC 
GCGATCGCAC ACTCCTTCAC GCCAAAGTCA ATTGGCCGCC TGGCCCGGGA GGTTGGCCTC 
TTCTCAGAGG AGGTGGAGCC CTACGGGACG ACCCGAGCCA AAGTTCGTCT GGAGGCCTTG 
AACCGCCTCA AGACACAGCC TAATGGGAAA TATTTGGTGG TCACAGGGAT CACACCCACA 
CCACTGGGTG AGGGAACTAC AACCACCACC CTGGGGTTGG CACAGGCCCT AGGGGCACAC 
CTTCATGTCA ACAGTTTTGC CTGTGTGCGC CAACCCAGCC GGGGCTCAAA CTTTGGGGTC 
AAAGGTGGTG CTGTTGGAGG TGGTTATTGC CAAGTCATTC CTATGGAAGA GGTCAGTCTC 
CACCTCACCA GTGACACACA AGCTGTCATG GCAGCCAGTA GCCTGGTGGT GGACACTATC 
AATGCACGTG TGTTATGCGA GTCAACACAG TCAGATAAGG CTCTCTTTGA ATGGCTGGTG 
CCACTGAGGG ACGGTCACAG GAAGTTCTCC CTGTCCCAAC TCAATAGGTT GAAGAGACTG 
GGTATAGAGA AACCAGAAAC CCTGAAACCA GAGGAGATAT ACCGATTTAT CCGTTTGGAC 
ATTGATCCAG AGACCAAGAC GCTGAGTGGA TATGTGGCCA GTGAGATGAT GGCGGTGTTG 
GCCCTCAGTA CCAGTCTGGC AGACATGACG CGCCGCTTGG CCAGGATGGT GGTGGCCTAC 
AGCCGCAAGG GGAAGCCTGT CACAACAGAG GACCTGGGTA TTAGTGGAGT GCTGGCCACA 
TTGATGAGAG ATGCTGTGAA GCCCAGTCTA ATGCAGACCA TGGAGGGGAC ACCTGTGTTT 
GTTCACACCT GTCCATTGTC AGATATTGCC CAGGGCAACT CGTCCATCCT GGCTGACCAG 
ATCGCCTTAA AGCTGGTGGG CCCAGAGGGG TTTGTGGTCA CCGAGGCAGA AGGAGGTGCT 
GAATTAGGCA TGGAGAAGTT CTTCGACATC AAGTGTCGCT CTTCGGGCCT GCATCCTGAC 
GTGGTGGTGA TGGTGGCTTC AGTTCCTGCC CTGAAGATGC ATGGGGGTGG ACCTGCGGTC 
ACGGCAGGTT CAGCCATGCC GAAGGAGTAC TCAGCGGAGA ACCTGACGTT ACTGGAAAAT 
GGCTGCAACC ACTTGAAGAG GCAGCTGGAG AATGCCCGGG CTTTTGGCTT ACCTGTCGTT 
GTAGCCATCA ACACCTTCAG CACCGACACT GATGCAGAGT TGGGCCTAGT CTGTGAGCAG 
GCTAAACTGG CGGGGGCCTT GGAGGCGGTG CCATGCTCAC ACTGGGCTGA AGGGGGTGCC 
GGAGCGGTGG CGTTGGGTCA GGCGGTACAG AGGGCAGCTG AGACACCACA CCAGATTAAC 
TTCCTGTATG ACCTGGAGAT GCCCATTGAC GACAAAATCA GAGTAATAGC AAAGTCAATG 
TATGGAGCGG ATGATGTGGA GCTCCTTCCT CAGGCCCAAC AGAAGGTGGC GCTGTTCTCA 
AAACAAGGCT TGGGCAACCT GCCCATATGC ATGGCAAAGA CCCACCTGTC CCTGTCCCAC 
GACCCAGAGA GGAAAGGGGT GCCCACCGGC TTTACCTTGC CCATCAGGGA CATCCATGCC 
AATTTGGGTG CTGGTTTTCT CTACCCCTTG GTTGGCACGT CGAGTGTAGC ACCCCAATCT 
CCAGCCTTCT CCTGTTTCCA TGACAACGAC TTCAGCACTG AGAGCAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC110521990 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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