nrap cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	nrap
Entrez Gene ID	110498821
Full Name	nebulin related anchoring protein
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021575458.1	XP_021431133.1	nebulin-related-anchoring protein isoform X2
XM_021575459.1	XP_021431134.1	nebulin-related-anchoring protein isoform X3
XM_021575462.1	XP_021431137.1	nebulin-related-anchoring protein isoform X6
XM_021575457.1	XP_021431132.1	nebulin-related-anchoring protein isoform X1
XM_021575460.1	XP_021431135.1	nebulin-related-anchoring protein isoform X4
XM_021575461.1	XP_021431136.1	nebulin-related-anchoring protein isoform X5
XM_021575464.1	XP_021431139.1	nebulin-related-anchoring protein isoform X7

Pathways from BioSystems
Gene Ontology

The following nrap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nrap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OOf25628	XM_021575457.1 Latest version!	Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf25629	XM_021575458.1 Latest version!	Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf25630	XM_021575459.1 Latest version!	Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf25631	XM_021575460.1 Latest version!	Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf25632	XM_021575461.1 Latest version!	Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf25633	XM_021575462.1 Latest version!	Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OOf25634	XM_021575464.1 Latest version!	Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nrap ( XM_021575457.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021431132.1 Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf25628
Clone ID Related Accession (Same CDS sequence)	XM_021575457.1
Accession Version	XM_021575457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	nebulin-related-anchoring protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT GCGCCAGGTG TGGGTTTGTG GTGTACCCAG CGGAGAAGAT CAATTGTATT 
GATCAGAACT GGCACAAAGC ATGCTTTCAC TGCGACGTCT GTAAAATGGT GCTCACAGCC 
AAGAACTTTG TTAGCCACAA GAAGCGGCCA TACTGCTCTG TACACAATCC AAGAAACAAT 
ATGTTCACCA GTGTCTATGA GACCCCCATC AATGCCAAGA AGCAAGCCAT TGCCAGCAGT 
GAGCAGGACT ATCGGGCTAA GTTTGAGGAG CAGCAGACCT ACTCGTACTC AGGCACCATC 
ACCAACCAGG AGATTGTGAG CGTGACACAG GCCCAAAAGA CCATCAGTGA TGTCAAATAC 
ACTGAGGAAT ATGAGCAGAG TAAAGGAAAA GGCAGCTTAT CCACCATGAT CACACCAGGA 
TACCAGATTT CCAAGAAACC AAATACCTTG CCCAGCAGCG CGGAGTACAA TAAAGGAGAG 
GAGAAAGTGT CAAAATATTC CTCTGTAGCC AAAACCCCTG AAGTGCTTCT GGCCAAAAGC 
CAAGGACAGA TCCATTATGT CTACACTGAA GAGTATGAAC AGCAGTTGGG TAAAGGCAGT 
TTCCCCGCAC ATCTCACTCC TGGCTACCAA GTTTCAAAGA AAGCCACAGA GATGGCCAGT 
GGTGTGAAGT ACCGTCAGAT GTATGAGCAG GAGATGAAGG GAAAGGCCAC CTCAGATGCT 
GGGGCAGCAG AATTCGCTTC TGCCAGAGAA AATGCTGGGA ACTTCAGCCA GATTGCCTAC 
ACTGAAGAGT ACGAGCAGCA GAGAGGGAAG GGCAGTTTCC CAGCCATGAT CACTCCTGCC 
TACCAGCTTG CCAAGAAAGC CCAGGCCCAC GCCAGTGATC TGAAATACAA GAAGGACCTG 
AATAAGATGA AGGGCTCATC CCACTTCCAC AACCTGACCA GTGAGGACAA CCTGGCCCTG 
AAGAGCGCCC GCAAAATCAA CAAGATCGTC AGCGAGGTTG AGTACAAGAA GGACCTTGAG 
AACACTAAAG GCCACAGCAT CAACTACTGT GAGACGCCCC AGTACAAGAA TGCATCCAAG 
GCTGCCAAGT TCAGCAGTGA TAACAAGTAC AAGGAGAAGT ATACCAACCA CATGAAAGGG 
CACTATGAAG GCTCTGAGAT GGACAAGAAT GCTGTACACG CTATGAACGT CAGAAAGCTG 
GCAAGCAATA TTGCCTACAA GCAGGAGTAT GAGCAGGAGA AAACTGACCA TGTAGAATAC 
AACTACCCAG CCACCATCAC ACCAGGCTAC CAGAGTCAGA GGAAACTGGA TCCACTCAAG 
GACAAGAACT ACAGACAGCA CATTGACCAA GTCAAGTACA GCTCGGTGAC AGATACCCCT 
GACATCGTCC AAGCCAGGAT CAACGCTCAA CAACTCAGCA ATGTTAACTA CAGACAACAC 
ATTGACCAAG TCAAGTACAG CTCGGTGACA GATACCCCTC AAATCGTCCA GGCCAGGATC 
AACGCTCAAC AACTCAGCAA TATAAAATAC AAGGAGGACT GGGAGAAGAC CAAGTCCAAA 
ACATGTGACA TCGGCCTGGA CACGCTGACC GTCAAGGCTG CCAAAGCATC TCGGGACCTG 
GCCAGTGATA TTAAGTACAA AGAGTCATTC ATGAAAAATA AAGATAAGAC AAGCAATGTC 
AGCGACTCCA AGACCCTGCA CTCTCTTCAG GCGTCCAAGA TGACCAGTGA GATTGAGTAC 
AAGAAGAACT CCAAGGAGAA CCAGTCCCAG TTCAGCTTGT CCATGGACAT GGTCAACCTG 
AGCCATGCTA AAAAAGCCCA GGCCCTTGCC AGCGACCTGG ACTACAGGAC GAAGCTCCAC 
CACTACACCA TCCTATCCGA CGACATCCAA GTACAGCAGG CCAAAAAGGC CTATAACCTG 
CAGAGCGAGA ATCAATACCG TTCAGATCTG ACCTGGATGA AGGGAGTTGG CTGGGAGGCT 
GATGGATCTC TGAATGTGAC CCAAGCCAAG AAGGCTGGAG AGCTTCTCAG TGACAAAAAG 
TACCGTCAGA AAGTGGACGA CGTCAAGTTC ACCCAGGTGG CCGATACTCC CTCTATCAAA 
CACGCCAAGA AGAGCCAGGA ACTGCAGAGC AAGCTAACGT ATAAGGCGGG CAACGAACAG 
ATGATGCACC AGTACACCAT GGATAAAGAT GAGCCACTCT TCAGGCAGGC CAAAGCCAAT 
GCAGACCTCC TCAGTGGGAA AGTGTATAAG AGTAGCTGGG AGCAACAGCG AGAAAAGGGC 
TTTGAGTTGC GCATGGACTC CTTGTCCATC CTCACAGCTA AGGCTAAGAG AGACCTGGCC 
AGTAACGTGA AATACAAGGA GAAGTACGAG CAGACTAAGG GGAAGATGAT TGGGGTGAAG 
ATGGTCACTG ACGACTCTCA GATGGCGCAC TCGGCTCTGG CCACCCGGTT GCAGAGTGAT 
CGCCACTACA GAAAGAAGTA CGAGGACACT AAAACCAAGC ATGGTGCCTC TTTGGACATG 
TTGACCATGA CCCACGCCAA GAAGGCCCAG GATCTGGCAA CTGAGACCAA CTACAGGACC 
TTCCTGCACA AGTACACAAC CCTGCCCGGC GACATGAAGG TGGCCTGGGC CAAGAAGGCC 
TACGGACTAC TGAGTGATAA ACAGTACAGG TCGGATCGGA ACTGGATGAA GGGCGTGGGC 
TGGGTGGCAG CAGATTTCCT GGATGTGAAA CAAGCAAAGA AGGCTGGCGA GCTCGTTAGC 
GAGAAAAAGT ACCGTCAACA TGTCAGCGAT CTGAAGTTCA CCAGCGTGGA GGACACTCCC 
GAGATGGTCC AGGCCAAACT CAGCAACAAA CTGGCCCTAG ACAGGCTGTA CAGGGAAAAG 
GGGGAACATA TGAAGCACAA CTACACACTC TCCGGAGATC TGCCTGAACA CGTGCAGGCC 
AAGCTCAATG CCATGAACAT CAGTGAGACC CGCTATAAGG AGTCGTGGAC TAAAATCAGA 
GATGGTGGCT ACAAGCTCCG TTTGGATGCC ATTCCCTTCC AGTCAGCCAA AGCGTCTGGG 
AACATACTTA GTGATCACAA GTACAAGGAG CAGTTTGAAA AGACCAAGGG GCATATGATT 
GGACTGAAGG GACTGGAGAA CGACATGAAC ATGTCTCACT CTGTGAATGC CAGTAAACTG 
CAGAGTGACA TCAAGTATAA GCAGGACTCT GTGAATAAGC GCTCTCAGTA CCACCTTTCT 
ATGGACATGT TGGAGATGGC CCATGCTAAA AAGGCCCAGA CCCTGGCCAG TGAGCAGGAC 
TACCGGCTTA CACTCCACCA GTACACTACT CAACCCGATG ACATGAAGGT GCAGGCCGCT 
AAGAGAGCCT ACCAACTACA GAGCGAGAAA GTGTACCGCT CTGACCTGAA CTACCTGCGT 
GGTGTAGCCT GGATCAGCAC CGGGGCCCTC CAGATGGAGG GCTTCAAAAG GGCCACAGAC 
CTTATTAGTG AGAAAAACTA CCGTCAGCAG CCCTGCTCCT TCAAGCACAC ATCTGTCGCA 
GACTCACCAG ACATTGTCCA TGCCAAACTC AGCAATAAGC TTACCAACGA GCGCCTGTAC 
AAGATGAAAG GGGAGAATGA CAGGCATAAC TACACAATGA CATTGGAGAG ACCAGAGATA 
ACACAGGCTA AGATCAACGC GGCTAACTTC AGCGAGACCA AGTACAGGGA GTCATGGCAC 
ACTCTAAGGG CTCAGGGCTA CAAACTGACC ATGCAGGACA TCCCCTTCCA GGCTGCCAAA 
ACCTCAATGG ATTTAGCCAG CGACGTCCAA TACAAGCACC ACCACCTGCT GGATAAGGGC 
AAGCACATCG GCGCCCGCAG CATTCTGGAT GACCCGCGCA TGCTACACTG CATGCAGATG 
GGCCGGCTGA CGAGTGAGCA GGAGTACCGC AAAGACGCAC TTTCCACCAG CGGCCAGTAC 
CACCTCAATC CAGATATGAT CAATTTAGTG ACAGCCAAGA ACGCCCAAGC TCTGGCCAGC 
GATCAGGACT ACAGGAAGAG GCTCCATGCG TACACTGTCC TCCCTGACGA TATTAAAGTC 
CAATGGGCCA AGAAGGCCTA TAACCTGCAG AGCGAGAAAC TGTACAAGTC GGATCTGAAC 
TTCATGAAGG GCGTGGCCTG GGATGGTGTG GGTGCGCCTC AAATGGAGTT GGCAAAGAAA 
GCCGGAGAAC TCACCAGTAA TAAGAAATAT CGTCAACTGC CAGACAGTCT GAAGTTCACC 
CAAGTGGCGG ACTCTCCAGA CATTGTTCAT GCTAAGAACA GCTACCTCCA GTGCAGTGAG 
AGGCTGTATA AGTCTGGAGA CTCGGACGCC ATGCACAAAT ACACCTTACA TGCCGACGAC 
CCAGACTTCA TCAGAGCAAG GATCAACGCC CAGCAGATTA GCAATAAAGC CTATAAAGCG 
TCTGGGGATG AGATGAGGTC GGCAGGCTAC GACTTGAGGC TGGATGCTAT TCCCTTCCAG 
ACGGCCAAGG CTTCCACGAA TATCGCCAGT GATTTCCGCT ACAAGGAAGC CTTTGTGAAG 
GAGAAGGGTC AGCAGGTGGG GCTGCTCAGT GTCGAAGATG ACCCAAAGAT GGTGCACTCC 
CTGGCAGCCA GCAAGCTCCA GAGCTGCCTG GGGTACAAGA GGCAGGCTCA AGAGACGCGC 
TCCCAGTTCA AGATGCATGC CAACCAGCCT GTGTTCCAGC AGGCCAAGAA GAGCCAAGCT 
CTGGCCAGCA ACCAGACCTA TCACCAGAGG CTCCATGACT ACACCTTTGA CCCCCAGCAG 
CTCAACGTCC AACACGCCAA GCAGGCCTAC AAGCTGCAGA GTGACGTGAA CTACAAGTCA 
GATCTGAACT GGATCAGGGG TGTGGGCTGG ACCCCCCCTG GCTCCCATAA GGTGGAGATG 
GCCCGGCGGG CTGCAGAACT GGGCCTGACG GAGAGCGTGA GCACTGAGGA GGCCATTGCC 
CAGTACCAAC ACATGATGAT GATGCAGCAG ATGGAGGAGC AGCAGCAGCT GTCAGAACCT 
GTTGAGACCA GCAAAGAGTT CCAGCAGTCG GGAGTCAATC TAGACGCAAT GGAGATCCTC 
CACGTCAAGA GGAAGAAGAC CATCCAAACG GTGAAGAAGT CCAATTCCTC CTCCATGGAT 
AGCAGCGTCA AGAAGTTAAT GATGTCTTCC AACTCAGCCA TAGAGTCAAT GATGGCTTCC 
ACCTCTGTCT CAGCCATTGA GAACACAGTC AAGATGTCCA CAACGAGTGA GGCATCGGCC 
ATAGAAAATG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021431132.1
CDS	198..5492
Translation	MQSCARCGFVVYPAEKINCIDQNWHKACFHCDVCKMVLTAKNFVSHKKRPYCSVHNPRNNMFTSVYETPINAKKQAIASSEQDYRAKFEEQQTYSYSGTITNQEIVSVTQAQKTISDVKYTEEYEQSKGKGSLSTMITPGYQISKKPNTLPSSAEYNKGEEKVSKYSSVAKTPEVLLAKSQGQIHYVYTEEYEQQLGKGSFPAHLTPGYQVSKKATEMASGVKYRQMYEQEMKGKATSDAGAAEFASARENAGNFSQIAYTEEYEQQRGKGSFPAMITPAYQLAKKAQAHASDLKYKKDLNKMKGSSHFHNLTSEDNLALKSARKINKIVSEVEYKKDLENTKGHSINYCETPQYKNASKAAKFSSDNKYKEKYTNHMKGHYEGSEMDKNAVHAMNVRKLASNIAYKQEYEQEKTDHVEYNYPATITPGYQSQRKLDPLKDKNYRQHIDQVKYSSVTDTPDIVQARINAQQLSNVNYRQHIDQVKYSSVTDTPQIVQARINAQQLSNIKYKEDWEKTKSKTCDIGLDTLTVKAAKASRDLASDIKYKESFMKNKDKTSNVSDSKTLHSLQASKMTSEIEYKKNSKENQSQFSLSMDMVNLSHAKKAQALASDLDYRTKLHHYTILSDDIQVQQAKKAYNLQSENQYRSDLTWMKGVGWEADGSLNVTQAKKAGELLSDKKYRQKVDDVKFTQVADTPSIKHAKKSQELQSKLTYKAGNEQMMHQYTMDKDEPLFRQAKANADLLSGKVYKSSWEQQREKGFELRMDSLSILTAKAKRDLASNVKYKEKYEQTKGKMIGVKMVTDDSQMAHSALATRLQSDRHYRKKYEDTKTKHGASLDMLTMTHAKKAQDLATETNYRTFLHKYTTLPGDMKVAWAKKAYGLLSDKQYRSDRNWMKGVGWVAADFLDVKQAKKAGELVSEKKYRQHVSDLKFTSVEDTPEMVQAKLSNKLALDRLYREKGEHMKHNYTLSGDLPEHVQAKLNAMNISETRYKESWTKIRDGGYKLRLDAIPFQSAKASGNILSDHKYKEQFEKTKGHMIGLKGLENDMNMSHSVNASKLQSDIKYKQDSVNKRSQYHLSMDMLEMAHAKKAQTLASEQDYRLTLHQYTTQPDDMKVQAAKRAYQLQSEKVYRSDLNYLRGVAWISTGALQMEGFKRATDLISEKNYRQQPCSFKHTSVADSPDIVHAKLSNKLTNERLYKMKGENDRHNYTMTLERPEITQAKINAANFSETKYRESWHTLRAQGYKLTMQDIPFQAAKTSMDLASDVQYKHHHLLDKGKHIGARSILDDPRMLHCMQMGRLTSEQEYRKDALSTSGQYHLNPDMINLVTAKNAQALASDQDYRKRLHAYTVLPDDIKVQWAKKAYNLQSEKLYKSDLNFMKGVAWDGVGAPQMELAKKAGELTSNKKYRQLPDSLKFTQVADSPDIVHAKNSYLQCSERLYKSGDSDAMHKYTLHADDPDFIRARINAQQISNKAYKASGDEMRSAGYDLRLDAIPFQTAKASTNIASDFRYKEAFVKEKGQQVGLLSVEDDPKMVHSLAASKLQSCLGYKRQAQETRSQFKMHANQPVFQQAKKSQALASNQTYHQRLHDYTFDPQQLNVQHAKQAYKLQSDVNYKSDLNWIRGVGWTPPGSHKVEMARRAAELGLTESVSTEEAIAQYQHMMMMQQMEEQQQLSEPVETSKEFQQSGVNLDAMEILHVKRKKTIQTVKKSNSSSMDSSVKKLMMSSNSAIESMMASTSVSAIENTVKMSTTSEASAIENA

Target ORF information:

RefSeq Version	XM_021575457.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021575457.1

ORF Insert Sequence:

ATGCAGTCCT GCGCCAGGTG TGGGTTTGTG GTGTACCCAG CGGAGAAGAT CAATTGTATT 
GATCAGAACT GGCACAAAGC ATGCTTTCAC TGCGACGTCT GTAAAATGGT GCTCACAGCC 
AAGAACTTTG TTAGCCACAA GAAGCGGCCA TACTGCTCTG TACACAATCC AAGAAACAAT 
ATGTTCACCA GTGTCTATGA GACCCCCATC AATGCCAAGA AGCAAGCCAT TGCCAGCAGT 
GAGCAGGACT ATCGGGCTAA GTTTGAGGAG CAGCAGACCT ACTCGTACTC AGGCACCATC 
ACCAACCAGG AGATTGTGAG CGTGACACAG GCCCAAAAGA CCATCAGTGA TGTCAAATAC 
ACTGAGGAAT ATGAGCAGAG TAAAGGAAAA GGCAGCTTAT CCACCATGAT CACACCAGGA 
TACCAGATTT CCAAGAAACC AAATACCTTG CCCAGCAGCG CGGAGTACAA TAAAGGAGAG 
GAGAAAGTGT CAAAATATTC CTCTGTAGCC AAAACCCCTG AAGTGCTTCT GGCCAAAAGC 
CAAGGACAGA TCCATTATGT CTACACTGAA GAGTATGAAC AGCAGTTGGG TAAAGGCAGT 
TTCCCCGCAC ATCTCACTCC TGGCTACCAA GTTTCAAAGA AAGCCACAGA GATGGCCAGT 
GGTGTGAAGT ACCGTCAGAT GTATGAGCAG GAGATGAAGG GAAAGGCCAC CTCAGATGCT 
GGGGCAGCAG AATTCGCTTC TGCCAGAGAA AATGCTGGGA ACTTCAGCCA GATTGCCTAC 
ACTGAAGAGT ACGAGCAGCA GAGAGGGAAG GGCAGTTTCC CAGCCATGAT CACTCCTGCC 
TACCAGCTTG CCAAGAAAGC CCAGGCCCAC GCCAGTGATC TGAAATACAA GAAGGACCTG 
AATAAGATGA AGGGCTCATC CCACTTCCAC AACCTGACCA GTGAGGACAA CCTGGCCCTG 
AAGAGCGCCC GCAAAATCAA CAAGATCGTC AGCGAGGTTG AGTACAAGAA GGACCTTGAG 
AACACTAAAG GCCACAGCAT CAACTACTGT GAGACGCCCC AGTACAAGAA TGCATCCAAG 
GCTGCCAAGT TCAGCAGTGA TAACAAGTAC AAGGAGAAGT ATACCAACCA CATGAAAGGG 
CACTATGAAG GCTCTGAGAT GGACAAGAAT GCTGTACACG CTATGAACGT CAGAAAGCTG 
GCAAGCAATA TTGCCTACAA GCAGGAGTAT GAGCAGGAGA AAACTGACCA TGTAGAATAC 
AACTACCCAG CCACCATCAC ACCAGGCTAC CAGAGTCAGA GGAAACTGGA TCCACTCAAG 
GACAAGAACT ACAGACAGCA CATTGACCAA GTCAAGTACA GCTCGGTGAC AGATACCCCT 
GACATCGTCC AAGCCAGGAT CAACGCTCAA CAACTCAGCA ATGTTAACTA CAGACAACAC 
ATTGACCAAG TCAAGTACAG CTCGGTGACA GATACCCCTC AAATCGTCCA GGCCAGGATC 
AACGCTCAAC AACTCAGCAA TATAAAATAC AAGGAGGACT GGGAGAAGAC CAAGTCCAAA 
ACATGTGACA TCGGCCTGGA CACGCTGACC GTCAAGGCTG CCAAAGCATC TCGGGACCTG 
GCCAGTGATA TTAAGTACAA AGAGTCATTC ATGAAAAATA AAGATAAGAC AAGCAATGTC 
AGCGACTCCA AGACCCTGCA CTCTCTTCAG GCGTCCAAGA TGACCAGTGA GATTGAGTAC 
AAGAAGAACT CCAAGGAGAA CCAGTCCCAG TTCAGCTTGT CCATGGACAT GGTCAACCTG 
AGCCATGCTA AAAAAGCCCA GGCCCTTGCC AGCGACCTGG ACTACAGGAC GAAGCTCCAC 
CACTACACCA TCCTATCCGA CGACATCCAA GTACAGCAGG CCAAAAAGGC CTATAACCTG 
CAGAGCGAGA ATCAATACCG TTCAGATCTG ACCTGGATGA AGGGAGTTGG CTGGGAGGCT 
GATGGATCTC TGAATGTGAC CCAAGCCAAG AAGGCTGGAG AGCTTCTCAG TGACAAAAAG 
TACCGTCAGA AAGTGGACGA CGTCAAGTTC ACCCAGGTGG CCGATACTCC CTCTATCAAA 
CACGCCAAGA AGAGCCAGGA ACTGCAGAGC AAGCTAACGT ATAAGGCGGG CAACGAACAG 
ATGATGCACC AGTACACCAT GGATAAAGAT GAGCCACTCT TCAGGCAGGC CAAAGCCAAT 
GCAGACCTCC TCAGTGGGAA AGTGTATAAG AGTAGCTGGG AGCAACAGCG AGAAAAGGGC 
TTTGAGTTGC GCATGGACTC CTTGTCCATC CTCACAGCTA AGGCTAAGAG AGACCTGGCC 
AGTAACGTGA AATACAAGGA GAAGTACGAG CAGACTAAGG GGAAGATGAT TGGGGTGAAG 
ATGGTCACTG ACGACTCTCA GATGGCGCAC TCGGCTCTGG CCACCCGGTT GCAGAGTGAT 
CGCCACTACA GAAAGAAGTA CGAGGACACT AAAACCAAGC ATGGTGCCTC TTTGGACATG 
TTGACCATGA CCCACGCCAA GAAGGCCCAG GATCTGGCAA CTGAGACCAA CTACAGGACC 
TTCCTGCACA AGTACACAAC CCTGCCCGGC GACATGAAGG TGGCCTGGGC CAAGAAGGCC 
TACGGACTAC TGAGTGATAA ACAGTACAGG TCGGATCGGA ACTGGATGAA GGGCGTGGGC 
TGGGTGGCAG CAGATTTCCT GGATGTGAAA CAAGCAAAGA AGGCTGGCGA GCTCGTTAGC 
GAGAAAAAGT ACCGTCAACA TGTCAGCGAT CTGAAGTTCA CCAGCGTGGA GGACACTCCC 
GAGATGGTCC AGGCCAAACT CAGCAACAAA CTGGCCCTAG ACAGGCTGTA CAGGGAAAAG 
GGGGAACATA TGAAGCACAA CTACACACTC TCCGGAGATC TGCCTGAACA CGTGCAGGCC 
AAGCTCAATG CCATGAACAT CAGTGAGACC CGCTATAAGG AGTCGTGGAC TAAAATCAGA 
GATGGTGGCT ACAAGCTCCG TTTGGATGCC ATTCCCTTCC AGTCAGCCAA AGCGTCTGGG 
AACATACTTA GTGATCACAA GTACAAGGAG CAGTTTGAAA AGACCAAGGG GCATATGATT 
GGACTGAAGG GACTGGAGAA CGACATGAAC ATGTCTCACT CTGTGAATGC CAGTAAACTG 
CAGAGTGACA TCAAGTATAA GCAGGACTCT GTGAATAAGC GCTCTCAGTA CCACCTTTCT 
ATGGACATGT TGGAGATGGC CCATGCTAAA AAGGCCCAGA CCCTGGCCAG TGAGCAGGAC 
TACCGGCTTA CACTCCACCA GTACACTACT CAACCCGATG ACATGAAGGT GCAGGCCGCT 
AAGAGAGCCT ACCAACTACA GAGCGAGAAA GTGTACCGCT CTGACCTGAA CTACCTGCGT 
GGTGTAGCCT GGATCAGCAC CGGGGCCCTC CAGATGGAGG GCTTCAAAAG GGCCACAGAC 
CTTATTAGTG AGAAAAACTA CCGTCAGCAG CCCTGCTCCT TCAAGCACAC ATCTGTCGCA 
GACTCACCAG ACATTGTCCA TGCCAAACTC AGCAATAAGC TTACCAACGA GCGCCTGTAC 
AAGATGAAAG GGGAGAATGA CAGGCATAAC TACACAATGA CATTGGAGAG ACCAGAGATA 
ACACAGGCTA AGATCAACGC GGCTAACTTC AGCGAGACCA AGTACAGGGA GTCATGGCAC 
ACTCTAAGGG CTCAGGGCTA CAAACTGACC ATGCAGGACA TCCCCTTCCA GGCTGCCAAA 
ACCTCAATGG ATTTAGCCAG CGACGTCCAA TACAAGCACC ACCACCTGCT GGATAAGGGC 
AAGCACATCG GCGCCCGCAG CATTCTGGAT GACCCGCGCA TGCTACACTG CATGCAGATG 
GGCCGGCTGA CGAGTGAGCA GGAGTACCGC AAAGACGCAC TTTCCACCAG CGGCCAGTAC 
CACCTCAATC CAGATATGAT CAATTTAGTG ACAGCCAAGA ACGCCCAAGC TCTGGCCAGC 
GATCAGGACT ACAGGAAGAG GCTCCATGCG TACACTGTCC TCCCTGACGA TATTAAAGTC 
CAATGGGCCA AGAAGGCCTA TAACCTGCAG AGCGAGAAAC TGTACAAGTC GGATCTGAAC 
TTCATGAAGG GCGTGGCCTG GGATGGTGTG GGTGCGCCTC AAATGGAGTT GGCAAAGAAA 
GCCGGAGAAC TCACCAGTAA TAAGAAATAT CGTCAACTGC CAGACAGTCT GAAGTTCACC 
CAAGTGGCGG ACTCTCCAGA CATTGTTCAT GCTAAGAACA GCTACCTCCA GTGCAGTGAG 
AGGCTGTATA AGTCTGGAGA CTCGGACGCC ATGCACAAAT ACACCTTACA TGCCGACGAC 
CCAGACTTCA TCAGAGCAAG GATCAACGCC CAGCAGATTA GCAATAAAGC CTATAAAGCG 
TCTGGGGATG AGATGAGGTC GGCAGGCTAC GACTTGAGGC TGGATGCTAT TCCCTTCCAG 
ACGGCCAAGG CTTCCACGAA TATCGCCAGT GATTTCCGCT ACAAGGAAGC CTTTGTGAAG 
GAGAAGGGTC AGCAGGTGGG GCTGCTCAGT GTCGAAGATG ACCCAAAGAT GGTGCACTCC 
CTGGCAGCCA GCAAGCTCCA GAGCTGCCTG GGGTACAAGA GGCAGGCTCA AGAGACGCGC 
TCCCAGTTCA AGATGCATGC CAACCAGCCT GTGTTCCAGC AGGCCAAGAA GAGCCAAGCT 
CTGGCCAGCA ACCAGACCTA TCACCAGAGG CTCCATGACT ACACCTTTGA CCCCCAGCAG 
CTCAACGTCC AACACGCCAA GCAGGCCTAC AAGCTGCAGA GTGACGTGAA CTACAAGTCA 
GATCTGAACT GGATCAGGGG TGTGGGCTGG ACCCCCCCTG GCTCCCATAA GGTGGAGATG 
GCCCGGCGGG CTGCAGAACT GGGCCTGACG GAGAGCGTGA GCACTGAGGA GGCCATTGCC 
CAGTACCAAC ACATGATGAT GATGCAGCAG ATGGAGGAGC AGCAGCAGCT GTCAGAACCT 
GTTGAGACCA GCAAAGAGTT CCAGCAGTCG GGAGTCAATC TAGACGCAAT GGAGATCCTC 
CACGTCAAGA GGAAGAAGAC CATCCAAACG GTGAAGAAGT CCAATTCCTC CTCCATGGAT 
AGCAGCGTCA AGAAGTTAAT GATGTCTTCC AACTCAGCCA TAGAGTCAAT GATGGCTTCC 
ACCTCTGTCT CAGCCATTGA GAACACAGTC AAGATGTCCA CAACGAGTGA GGCATCGGCC 
ATAGAAAATG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrap ( XM_021575458.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021431133.1 Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X2, mRNA.

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CloneID	OOf25629
Clone ID Related Accession (Same CDS sequence)	XM_021575458.1
Accession Version	XM_021575458.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	nebulin-related-anchoring protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT GCGCCAGGTG TGGGTTTGTG GTGTACCCAG CGGAGAAGAT CAATTGTATT 
GATCAGAACT GGCACAAAGC ATGCTTTCAC TGCGACGTCT GTAAAATGGT GCTCACAGCC 
AAGAACTTTG TTAGCCACAA GAAGCGGCCA TACTGCTCTG TACACAATCC AAGAAACAAT 
ATGTTCACCA GTGTCTATGA GACCCCCATC AATGCCAAGA AGCAAGCCAT TGCCAGCAGT 
GAGCAGGACT ATCGGGCTAA GTTTGAGGAG CAGCAGACCT ACTCGTACTC AGGCACCATC 
ACCAACCAGG AGATTGTGAG CGTGACACAG GCCCAAAAGA CCATCAGTGA TGTCAAATAC 
ACTGAGGAAT ATGAGCAGAG TAAAGGAAAA GGCAGCTTAT CCACCATGAT CACACCAGGA 
TACCAGATTT CCAAGAAACC AAATACCTTG CCCAGCAGCG CGGAGTACAA TAAAGGAGAG 
GAGAAAGTGT CAAAATATTC CTCTGTAGCC AAAACCCCTG AAGTGCTTCT GGCCAAAAGC 
CAAGGACAGA TCCATTATGT CTACACTGAA GAGTATGAAC AGCAGTTGGG TAAAGGCAGT 
TTCCCCGCAC ATCTCACTCC TGGCTACCAA GTTTCAAAGA AAGCCACAGA GATGGCCAGT 
GGTGTGAAGT ACCGTCAGAT GTATGAGCAG GAGATGAAGG GAAAGGCCAC CTCAGATGCT 
GGGGCAGCAG AATTCGCTTC TGCCAGAGAA AATGCTGGGA ACTTCAGCCA GATTGCCTAC 
ACTGAAGAGT ACGAGCAGCA GAGAGGGAAG GGCAGTTTCC CAGCCATGAT CACTCCTGCC 
TACCAGCTTG CCAAGAAAGC CCAGGCCCAC GCCAGTGATC TGAAATACAA GAAGGACCTG 
AATAAGATGA AGGGCTCATC CCACTTCCAC AACCTGACCA GTGAGGACAA CCTGGCCCTG 
AAGAGCGCCC GCAAAATCAA CAAGATCGTC AGCGAGGTTG AGTACAAGAA GGACCTTGAG 
AACACTAAAG GCCACAGCAT CAACTACTGT GAGACGCCCC AGTACAAGAA TGCATCCAAG 
GCTGCCAAGT TCAGCAGTGA TAACAAGTAC AAGGAGAAGT ATACCAACCA CATGAAAGGG 
CACTATGAAG GCTCTGAGAT GGACAAGAAT GCTGTACACG CTATGAACGT CAGAAAGCTG 
GCAAGCAATA TTGCCTACAA GCAGGAGTAT GAGCAGGAGA AAACTGACCA TGTAGAATAC 
AACTACCCAG CCACCATCAC ACCAGGCTAC CAGAGTCAGA GGAAACTGGA TCCACTCAAG 
GACAAGAACT ACAGACAGCA CATTGACCAA GTCAAGTACA GCTCGGTGAC AGATACCCCT 
GACATCGTCC AAGCCAGGAT CAACGCTCAA CAACTCAGCA ATGTTAACTA CAGACAACAC 
ATTGACCAAG TCAAGTACAG CTCGGTGACA GATACCCCTC AAATCGTCCA GGCCAGGATC 
AACGCTCAAC AACTCAGCAA TATAAAATAC AAGGAGGACT GGGAGAAGAC CAAGTCCAAA 
ACATGTGACA TCGGCCTGGA CACGCTGACC GTCAAGGCTG CCAAAGCATC TCGGGACCTG 
GCCAGTGATA TTAAGTACAA AGAGTCATTC ATGAAAAATA AAGATAAGAC AAGCAATGTC 
AGCGACTCCA AGACCCTGCA CTCTCTTCAG GCGTCCAAGA TGACCAGTGA GATTGAGTAC 
AAGAAGAACT CCAAGGAGAA CCAGTCCCAG TTCAGCTTGT CCATGGACAT GGTCAACCTG 
AGCCATGCTA AAAAAGCCCA GGCCCTTGCC AGCGACCTGG ACTACAGGAC GAAGCTCCAC 
CACTACACCA TCCTATCCGA CGACATCCAA GTACAGCAGG CCAAAAAGGC CTATAACCTG 
CAGAGCGAGA ATCAATACCG TTCAGATCTG ACCTGGATGA AGGGAGTTGG CTGGGAGGCT 
GATGGATCTC TGAATGTGAC CCAAGCCAAG AAGGCTGGAG AGCTTCTCAG TGACAAAAAG 
TACCGTCAGA AAGTGGACGA CGTCAAGTTC ACCCAGGTGG CCGATACTCC CTCTATCAAA 
CACGCCAAGA AGAGCCAGGA ACTGCAGAGC AAGCTAACGT ATAAGGCGGG CAACGAACAG 
ATGATGCACC AGTACACCAT GGATAAAGAT GAGCCACTCT TCAGGCAGGC CAAAGCCAAT 
GCAGACCTCC TCAGTGGGAA AGTGTATAAG AGTAGCTGGG AGCAACAGCG AGAAAAGGGC 
TTTGAGTTGC GCATGGACTC CTTGTCCATC CTCACAGCTA AGGCTAAGAG AGACCTGGCC 
AGTAACGTGA AATACAAGGA GAAGTACGAG CAGACTAAGG GGAAGATGAT TGGGGTGAAG 
ATGGTCACTG ACGACTCTCA GATGGCGCAC TCGGCTCTGG CCACCCGGTT GCAGAGTGAT 
CGCCACTACA GAAAGAAGTA CGAGGACACT AAAACCAAGC ATGGTGCCTC TTTGGACATG 
TTGACCATGA CCCACGCCAA GAAGGCCCAG GATCTGGCAA CTGAGACCAA CTACAGGACC 
TTCCTGCACA AGTACACAAC CCTGCCCGGC GACATGAAGG TGGCCTGGGC CAAGAAGGCC 
TACGGACTAC TGAGTGATAA ACAGTACAGG TCGGATCGGA ACTGGATGAA GGGCGTGGGC 
TGGGTGGCAG CAGATTTCCT GGATGTGAAA CAAGCAAAGA AGGCTGGCGA GCTCGTTAGC 
GAGAAAAAGT ACCGTCAACA TGTCAGCGAT CTGAAGTTCA CCAGCGTGGA GGACACTCCC 
GAGATGGTCC AGGCCAAACT CAGCAACAAA CTGGCCCTAG ACAGGCTGTA CAGGGAAAAG 
GGGGAACATA TGAAGCACAA CTACACACTC TCCGGAGATC TGCCTGAACA CGTGCAGGCC 
AAGCTCAATG CCATGAACAT CAGTGAGACC CGCTATAAGG AGTCGTGGAC TAAAATCAGA 
GATGGTGGCT ACAAGCTCCG TTTGGATGCC ATTCCCTTCC AGTCAGCCAA AGCGTCTGGG 
AACATACTTA GTGATCACAA GTACAAGGAG CAGTTTGAAA AGACCAAGGG GCATATGATT 
GGACTGAAGG GACTGGAGAA CGACATGAAC ATGTCTCACT CTGTGAATGC CAGTAAACTG 
CAGAGTGACA TCAAGTATAA GCAGGACTCT GTGAATAAGC GCTCTCAGTA CCACCTTTCT 
ATGGACATGT TGGAGATGGC CCATGCTAAA AAGGCCCAGA CCCTGGCCAG TGAGCAGGAC 
TACCGGCTTA CACTCCACCA GTACACTACT CAACCCGATG ACATGAAGGT GCAGGCCGCT 
AAGAGAGCCT ACCAACTACA GAGCGAGAAA GTGTACCGCT CTGACCTGAA CTACCTGCGT 
GGTGTAGCCT GGATCAGCAC CGGGGCCCTC CAGATGGAGG GCTTCAAAAG GGCCACAGAC 
CTTATTAGTG AGAAAAACTA CCGTCAGCAG CCCTGCTCCT TCAAGCACAC ATCTGTCGCA 
GACTCACCAG ACATTGTCCA TGCCAAACTC AGCAATAAGC TTACCAACGA GCGCCTGTAC 
AAGATGAAAG GGGAGAATGA CAGGCATAAC TACACAATGA CATTGGAGAG ACCAGAGATA 
ACACAGGCTA AGATCAACGC GGCTAACTTC AGCGAGACCA AGTACAGGGA GTCATGGCAC 
ACTCTAAGGG CTCAGGGCTA CAAACTGACC ATGCAGGACA TCCCCTTCCA GGCTGCCAAA 
ACCTCAATGG ATTTAGCCAG CGACGTCCAA TACAAGCACC ACCACCTGCT GGATAAGGGC 
AAGCACATCG GCGCCCGCAG CATTCTGGAT GACCCGCGCA TGCTACACTG CATGCAGATG 
GGCCGGCTGA CGAGTGAGCA GGAGTACCGC AAAGACGCAC TTTCCACCAG CGGCCAGTAC 
CACCTCAATC CAGATATGAT CAATTTAGTG ACAGCCAAGA ACGCCCAAGC TCTGGCCAGC 
GATCAGGACT ACAGGAAGAG GCTCCATGCG TACACTGTCC TCCCTGACGA TATTAAAGTC 
CAATGGGCCA AGAAGGCCTA TAACCTGCAG AGCGAGAAAC TGTACAAGTC GGATCTGAAC 
TTCATGAAGG GCGTGGCCTG GGATGGTGTG GGTGCGCCTC AAATGGAGTT GGCAAAGAAA 
GCCGGAGAAC TCACCAGTAA TAAGAAATAT CGTCAACTGC CAGACAGTCT GAAGTTCACC 
CAAGTGGCGG ACTCTCCAGA CATTGTTCAT GCTAAGAACA GCTACCTCCA GTGCAGTGAG 
AGGCTGTATA AGTCTGGAGA CTCGGACGCC ATGCACAAAT ACACCTTACA TGCCGACGAC 
CCAGACTTCA TCAGAGCAAG GATCAACGCC CAGCAGATTA GCAATAAAGC CTATAAAGCG 
TCTGGGGATG AGATGAGGTC GGCAGGCTAC GACTTGAGGC TGGATGCTAT TCCCTTCCAG 
ACGGCCAAGG CTTCCACGAA TATCGCCAGT GATTTCCGCT ACAAGGAAGC CTTTGTGAAG 
GAGAAGGGTC AGCAGGTGGG GCTGCTCAGT GTCGAAGATG ACCCAAAGAT GGTGCACTCC 
CTGGCAGCCA GCAAGCTCCA GAGCTGCCTG GGGTACAAGA GGCAGGCTCA AGAGACGCGC 
TCCCAGTTCA AGATGCATGC CAACCAGCCT GTGTTCCAGC AGGCCAAGAA GAGCCAAGCT 
CTGGCCAGCA ACCAGACCTA TCACCAGAGG CTCCATGACT ACACCTTTGA CCCCCAGCAG 
CTCAACGTCC AACACGCCAA GCAGGCCTAC AAGCTGCAGA GTGACGTGAA CTACAAGTCA 
GATCTGAACT GGATCAGGGG TGTGGGCTGG ACCCCCCCTG GCTCCCATAA GGTGGAGATG 
GCCCGGCGGG CTGCAGAACT GGGCCTGACG GAGAGCGTGA GCACTGAGGA GGCCATTGCC 
CAGTACCAAC ACATGATGAT GATGCAGATG GAGGAGCAGC AGCAGCTGTC AGAACCTGTT 
GAGACCAGCA AAGAGTTCCA GCAGTCGGGA GTCAATCTAG ACGCAATGGA GATCCTCCAC 
GTCAAGAGGA AGAAGACCAT CCAAACGGTG AAGAAGTCCA ATTCCTCCTC CATGGATAGC 
AGCGTCAAGA AGTTAATGAT GTCTTCCAAC TCAGCCATAG AGTCAATGAT GGCTTCCACC 
TCTGTCTCAG CCATTGAGAA CACAGTCAAG ATGTCCACAA CGAGTGAGGC ATCGGCCATA 
GAAAATGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021431133.1
CDS	198..5489
Translation	MQSCARCGFVVYPAEKINCIDQNWHKACFHCDVCKMVLTAKNFVSHKKRPYCSVHNPRNNMFTSVYETPINAKKQAIASSEQDYRAKFEEQQTYSYSGTITNQEIVSVTQAQKTISDVKYTEEYEQSKGKGSLSTMITPGYQISKKPNTLPSSAEYNKGEEKVSKYSSVAKTPEVLLAKSQGQIHYVYTEEYEQQLGKGSFPAHLTPGYQVSKKATEMASGVKYRQMYEQEMKGKATSDAGAAEFASARENAGNFSQIAYTEEYEQQRGKGSFPAMITPAYQLAKKAQAHASDLKYKKDLNKMKGSSHFHNLTSEDNLALKSARKINKIVSEVEYKKDLENTKGHSINYCETPQYKNASKAAKFSSDNKYKEKYTNHMKGHYEGSEMDKNAVHAMNVRKLASNIAYKQEYEQEKTDHVEYNYPATITPGYQSQRKLDPLKDKNYRQHIDQVKYSSVTDTPDIVQARINAQQLSNVNYRQHIDQVKYSSVTDTPQIVQARINAQQLSNIKYKEDWEKTKSKTCDIGLDTLTVKAAKASRDLASDIKYKESFMKNKDKTSNVSDSKTLHSLQASKMTSEIEYKKNSKENQSQFSLSMDMVNLSHAKKAQALASDLDYRTKLHHYTILSDDIQVQQAKKAYNLQSENQYRSDLTWMKGVGWEADGSLNVTQAKKAGELLSDKKYRQKVDDVKFTQVADTPSIKHAKKSQELQSKLTYKAGNEQMMHQYTMDKDEPLFRQAKANADLLSGKVYKSSWEQQREKGFELRMDSLSILTAKAKRDLASNVKYKEKYEQTKGKMIGVKMVTDDSQMAHSALATRLQSDRHYRKKYEDTKTKHGASLDMLTMTHAKKAQDLATETNYRTFLHKYTTLPGDMKVAWAKKAYGLLSDKQYRSDRNWMKGVGWVAADFLDVKQAKKAGELVSEKKYRQHVSDLKFTSVEDTPEMVQAKLSNKLALDRLYREKGEHMKHNYTLSGDLPEHVQAKLNAMNISETRYKESWTKIRDGGYKLRLDAIPFQSAKASGNILSDHKYKEQFEKTKGHMIGLKGLENDMNMSHSVNASKLQSDIKYKQDSVNKRSQYHLSMDMLEMAHAKKAQTLASEQDYRLTLHQYTTQPDDMKVQAAKRAYQLQSEKVYRSDLNYLRGVAWISTGALQMEGFKRATDLISEKNYRQQPCSFKHTSVADSPDIVHAKLSNKLTNERLYKMKGENDRHNYTMTLERPEITQAKINAANFSETKYRESWHTLRAQGYKLTMQDIPFQAAKTSMDLASDVQYKHHHLLDKGKHIGARSILDDPRMLHCMQMGRLTSEQEYRKDALSTSGQYHLNPDMINLVTAKNAQALASDQDYRKRLHAYTVLPDDIKVQWAKKAYNLQSEKLYKSDLNFMKGVAWDGVGAPQMELAKKAGELTSNKKYRQLPDSLKFTQVADSPDIVHAKNSYLQCSERLYKSGDSDAMHKYTLHADDPDFIRARINAQQISNKAYKASGDEMRSAGYDLRLDAIPFQTAKASTNIASDFRYKEAFVKEKGQQVGLLSVEDDPKMVHSLAASKLQSCLGYKRQAQETRSQFKMHANQPVFQQAKKSQALASNQTYHQRLHDYTFDPQQLNVQHAKQAYKLQSDVNYKSDLNWIRGVGWTPPGSHKVEMARRAAELGLTESVSTEEAIAQYQHMMMMQMEEQQQLSEPVETSKEFQQSGVNLDAMEILHVKRKKTIQTVKKSNSSSMDSSVKKLMMSSNSAIESMMASTSVSAIENTVKMSTTSEASAIENA

Target ORF information:

RefSeq Version	XM_021575458.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021575458.1

ORF Insert Sequence:

ATGCAGTCCT GCGCCAGGTG TGGGTTTGTG GTGTACCCAG CGGAGAAGAT CAATTGTATT 
GATCAGAACT GGCACAAAGC ATGCTTTCAC TGCGACGTCT GTAAAATGGT GCTCACAGCC 
AAGAACTTTG TTAGCCACAA GAAGCGGCCA TACTGCTCTG TACACAATCC AAGAAACAAT 
ATGTTCACCA GTGTCTATGA GACCCCCATC AATGCCAAGA AGCAAGCCAT TGCCAGCAGT 
GAGCAGGACT ATCGGGCTAA GTTTGAGGAG CAGCAGACCT ACTCGTACTC AGGCACCATC 
ACCAACCAGG AGATTGTGAG CGTGACACAG GCCCAAAAGA CCATCAGTGA TGTCAAATAC 
ACTGAGGAAT ATGAGCAGAG TAAAGGAAAA GGCAGCTTAT CCACCATGAT CACACCAGGA 
TACCAGATTT CCAAGAAACC AAATACCTTG CCCAGCAGCG CGGAGTACAA TAAAGGAGAG 
GAGAAAGTGT CAAAATATTC CTCTGTAGCC AAAACCCCTG AAGTGCTTCT GGCCAAAAGC 
CAAGGACAGA TCCATTATGT CTACACTGAA GAGTATGAAC AGCAGTTGGG TAAAGGCAGT 
TTCCCCGCAC ATCTCACTCC TGGCTACCAA GTTTCAAAGA AAGCCACAGA GATGGCCAGT 
GGTGTGAAGT ACCGTCAGAT GTATGAGCAG GAGATGAAGG GAAAGGCCAC CTCAGATGCT 
GGGGCAGCAG AATTCGCTTC TGCCAGAGAA AATGCTGGGA ACTTCAGCCA GATTGCCTAC 
ACTGAAGAGT ACGAGCAGCA GAGAGGGAAG GGCAGTTTCC CAGCCATGAT CACTCCTGCC 
TACCAGCTTG CCAAGAAAGC CCAGGCCCAC GCCAGTGATC TGAAATACAA GAAGGACCTG 
AATAAGATGA AGGGCTCATC CCACTTCCAC AACCTGACCA GTGAGGACAA CCTGGCCCTG 
AAGAGCGCCC GCAAAATCAA CAAGATCGTC AGCGAGGTTG AGTACAAGAA GGACCTTGAG 
AACACTAAAG GCCACAGCAT CAACTACTGT GAGACGCCCC AGTACAAGAA TGCATCCAAG 
GCTGCCAAGT TCAGCAGTGA TAACAAGTAC AAGGAGAAGT ATACCAACCA CATGAAAGGG 
CACTATGAAG GCTCTGAGAT GGACAAGAAT GCTGTACACG CTATGAACGT CAGAAAGCTG 
GCAAGCAATA TTGCCTACAA GCAGGAGTAT GAGCAGGAGA AAACTGACCA TGTAGAATAC 
AACTACCCAG CCACCATCAC ACCAGGCTAC CAGAGTCAGA GGAAACTGGA TCCACTCAAG 
GACAAGAACT ACAGACAGCA CATTGACCAA GTCAAGTACA GCTCGGTGAC AGATACCCCT 
GACATCGTCC AAGCCAGGAT CAACGCTCAA CAACTCAGCA ATGTTAACTA CAGACAACAC 
ATTGACCAAG TCAAGTACAG CTCGGTGACA GATACCCCTC AAATCGTCCA GGCCAGGATC 
AACGCTCAAC AACTCAGCAA TATAAAATAC AAGGAGGACT GGGAGAAGAC CAAGTCCAAA 
ACATGTGACA TCGGCCTGGA CACGCTGACC GTCAAGGCTG CCAAAGCATC TCGGGACCTG 
GCCAGTGATA TTAAGTACAA AGAGTCATTC ATGAAAAATA AAGATAAGAC AAGCAATGTC 
AGCGACTCCA AGACCCTGCA CTCTCTTCAG GCGTCCAAGA TGACCAGTGA GATTGAGTAC 
AAGAAGAACT CCAAGGAGAA CCAGTCCCAG TTCAGCTTGT CCATGGACAT GGTCAACCTG 
AGCCATGCTA AAAAAGCCCA GGCCCTTGCC AGCGACCTGG ACTACAGGAC GAAGCTCCAC 
CACTACACCA TCCTATCCGA CGACATCCAA GTACAGCAGG CCAAAAAGGC CTATAACCTG 
CAGAGCGAGA ATCAATACCG TTCAGATCTG ACCTGGATGA AGGGAGTTGG CTGGGAGGCT 
GATGGATCTC TGAATGTGAC CCAAGCCAAG AAGGCTGGAG AGCTTCTCAG TGACAAAAAG 
TACCGTCAGA AAGTGGACGA CGTCAAGTTC ACCCAGGTGG CCGATACTCC CTCTATCAAA 
CACGCCAAGA AGAGCCAGGA ACTGCAGAGC AAGCTAACGT ATAAGGCGGG CAACGAACAG 
ATGATGCACC AGTACACCAT GGATAAAGAT GAGCCACTCT TCAGGCAGGC CAAAGCCAAT 
GCAGACCTCC TCAGTGGGAA AGTGTATAAG AGTAGCTGGG AGCAACAGCG AGAAAAGGGC 
TTTGAGTTGC GCATGGACTC CTTGTCCATC CTCACAGCTA AGGCTAAGAG AGACCTGGCC 
AGTAACGTGA AATACAAGGA GAAGTACGAG CAGACTAAGG GGAAGATGAT TGGGGTGAAG 
ATGGTCACTG ACGACTCTCA GATGGCGCAC TCGGCTCTGG CCACCCGGTT GCAGAGTGAT 
CGCCACTACA GAAAGAAGTA CGAGGACACT AAAACCAAGC ATGGTGCCTC TTTGGACATG 
TTGACCATGA CCCACGCCAA GAAGGCCCAG GATCTGGCAA CTGAGACCAA CTACAGGACC 
TTCCTGCACA AGTACACAAC CCTGCCCGGC GACATGAAGG TGGCCTGGGC CAAGAAGGCC 
TACGGACTAC TGAGTGATAA ACAGTACAGG TCGGATCGGA ACTGGATGAA GGGCGTGGGC 
TGGGTGGCAG CAGATTTCCT GGATGTGAAA CAAGCAAAGA AGGCTGGCGA GCTCGTTAGC 
GAGAAAAAGT ACCGTCAACA TGTCAGCGAT CTGAAGTTCA CCAGCGTGGA GGACACTCCC 
GAGATGGTCC AGGCCAAACT CAGCAACAAA CTGGCCCTAG ACAGGCTGTA CAGGGAAAAG 
GGGGAACATA TGAAGCACAA CTACACACTC TCCGGAGATC TGCCTGAACA CGTGCAGGCC 
AAGCTCAATG CCATGAACAT CAGTGAGACC CGCTATAAGG AGTCGTGGAC TAAAATCAGA 
GATGGTGGCT ACAAGCTCCG TTTGGATGCC ATTCCCTTCC AGTCAGCCAA AGCGTCTGGG 
AACATACTTA GTGATCACAA GTACAAGGAG CAGTTTGAAA AGACCAAGGG GCATATGATT 
GGACTGAAGG GACTGGAGAA CGACATGAAC ATGTCTCACT CTGTGAATGC CAGTAAACTG 
CAGAGTGACA TCAAGTATAA GCAGGACTCT GTGAATAAGC GCTCTCAGTA CCACCTTTCT 
ATGGACATGT TGGAGATGGC CCATGCTAAA AAGGCCCAGA CCCTGGCCAG TGAGCAGGAC 
TACCGGCTTA CACTCCACCA GTACACTACT CAACCCGATG ACATGAAGGT GCAGGCCGCT 
AAGAGAGCCT ACCAACTACA GAGCGAGAAA GTGTACCGCT CTGACCTGAA CTACCTGCGT 
GGTGTAGCCT GGATCAGCAC CGGGGCCCTC CAGATGGAGG GCTTCAAAAG GGCCACAGAC 
CTTATTAGTG AGAAAAACTA CCGTCAGCAG CCCTGCTCCT TCAAGCACAC ATCTGTCGCA 
GACTCACCAG ACATTGTCCA TGCCAAACTC AGCAATAAGC TTACCAACGA GCGCCTGTAC 
AAGATGAAAG GGGAGAATGA CAGGCATAAC TACACAATGA CATTGGAGAG ACCAGAGATA 
ACACAGGCTA AGATCAACGC GGCTAACTTC AGCGAGACCA AGTACAGGGA GTCATGGCAC 
ACTCTAAGGG CTCAGGGCTA CAAACTGACC ATGCAGGACA TCCCCTTCCA GGCTGCCAAA 
ACCTCAATGG ATTTAGCCAG CGACGTCCAA TACAAGCACC ACCACCTGCT GGATAAGGGC 
AAGCACATCG GCGCCCGCAG CATTCTGGAT GACCCGCGCA TGCTACACTG CATGCAGATG 
GGCCGGCTGA CGAGTGAGCA GGAGTACCGC AAAGACGCAC TTTCCACCAG CGGCCAGTAC 
CACCTCAATC CAGATATGAT CAATTTAGTG ACAGCCAAGA ACGCCCAAGC TCTGGCCAGC 
GATCAGGACT ACAGGAAGAG GCTCCATGCG TACACTGTCC TCCCTGACGA TATTAAAGTC 
CAATGGGCCA AGAAGGCCTA TAACCTGCAG AGCGAGAAAC TGTACAAGTC GGATCTGAAC 
TTCATGAAGG GCGTGGCCTG GGATGGTGTG GGTGCGCCTC AAATGGAGTT GGCAAAGAAA 
GCCGGAGAAC TCACCAGTAA TAAGAAATAT CGTCAACTGC CAGACAGTCT GAAGTTCACC 
CAAGTGGCGG ACTCTCCAGA CATTGTTCAT GCTAAGAACA GCTACCTCCA GTGCAGTGAG 
AGGCTGTATA AGTCTGGAGA CTCGGACGCC ATGCACAAAT ACACCTTACA TGCCGACGAC 
CCAGACTTCA TCAGAGCAAG GATCAACGCC CAGCAGATTA GCAATAAAGC CTATAAAGCG 
TCTGGGGATG AGATGAGGTC GGCAGGCTAC GACTTGAGGC TGGATGCTAT TCCCTTCCAG 
ACGGCCAAGG CTTCCACGAA TATCGCCAGT GATTTCCGCT ACAAGGAAGC CTTTGTGAAG 
GAGAAGGGTC AGCAGGTGGG GCTGCTCAGT GTCGAAGATG ACCCAAAGAT GGTGCACTCC 
CTGGCAGCCA GCAAGCTCCA GAGCTGCCTG GGGTACAAGA GGCAGGCTCA AGAGACGCGC 
TCCCAGTTCA AGATGCATGC CAACCAGCCT GTGTTCCAGC AGGCCAAGAA GAGCCAAGCT 
CTGGCCAGCA ACCAGACCTA TCACCAGAGG CTCCATGACT ACACCTTTGA CCCCCAGCAG 
CTCAACGTCC AACACGCCAA GCAGGCCTAC AAGCTGCAGA GTGACGTGAA CTACAAGTCA 
GATCTGAACT GGATCAGGGG TGTGGGCTGG ACCCCCCCTG GCTCCCATAA GGTGGAGATG 
GCCCGGCGGG CTGCAGAACT GGGCCTGACG GAGAGCGTGA GCACTGAGGA GGCCATTGCC 
CAGTACCAAC ACATGATGAT GATGCAGATG GAGGAGCAGC AGCAGCTGTC AGAACCTGTT 
GAGACCAGCA AAGAGTTCCA GCAGTCGGGA GTCAATCTAG ACGCAATGGA GATCCTCCAC 
GTCAAGAGGA AGAAGACCAT CCAAACGGTG AAGAAGTCCA ATTCCTCCTC CATGGATAGC 
AGCGTCAAGA AGTTAATGAT GTCTTCCAAC TCAGCCATAG AGTCAATGAT GGCTTCCACC 
TCTGTCTCAG CCATTGAGAA CACAGTCAAG ATGTCCACAA CGAGTGAGGC ATCGGCCATA 
GAAAATGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrap ( XM_021575459.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021431134.1 Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OOf25630
Clone ID Related Accession (Same CDS sequence)	XM_021575459.1
Accession Version	XM_021575459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	nebulin-related-anchoring protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT GCGCCAGGTG TGGGTTTGTG GTGTACCCAG CGGAGAAGAT CAATTGTATT 
GATCAGAACT GGCACAAAGC ATGCTTTCAC TGCGACGTCT GTAAAATGGT GCTCACAGCC 
AAGAACTTTG TTAGCCACAA GAAGCGGCCA TACTGCTCTG TACACAATCC AAGAAACAAT 
ATGTTCACCA GTGTCTATGA GACCCCCATC AATGCCAAGA AGCAAGCCAT TGCCAGCAGT 
GAGCAGGACT ATCGGGCTAA GTTTGAGGAG CAGCAGACCT ACTCGTACTC AGGCACCATC 
ACCAACCAGG AGATTGTGAG CGTGACACAG GCCCAAAAGA CCATCAGTGA TGTCAAATAC 
ACTGAGGAAT ATGAGCAGAG TAAAGGAAAA GGCAGCTTAT CCACCATGAT CACACCAGGA 
TACCAGATTT CCAAGAAACC AAATACCTTG CCCAGCAGCT ATGTCTACAC TGAAGAGTAT 
GAACAGCAGT TGGGTAAAGG CAGTTTCCCC GCACATCTCA CTCCTGGCTA CCAAGTTTCA 
AAGAAAGCCA CAGAGATGGC CAGTGGTGTG AAGTACCGTC AGATGTATGA GCAGGAGATG 
AAGGGAAAGG CCACCTCAGA TGCTGGGGCA GCAGAATTCG CTTCTGCCAG AGAAAATGCT 
GGGAACTTCA GCCAGATTGC CTACACTGAA GAGTACGAGC AGCAGAGAGG GAAGGGCAGT 
TTCCCAGCCA TGATCACTCC TGCCTACCAG CTTGCCAAGA AAGCCCAGGC CCACGCCAGT 
GATCTGAAAT ACAAGAAGGA CCTGAATAAG ATGAAGGGCT CATCCCACTT CCACAACCTG 
ACCAGTGAGG ACAACCTGGC CCTGAAGAGC GCCCGCAAAA TCAACAAGAT CGTCAGCGAG 
GTTGAGTACA AGAAGGACCT TGAGAACACT AAAGGCCACA GCATCAACTA CTGTGAGACG 
CCCCAGTACA AGAATGCATC CAAGGCTGCC AAGTTCAGCA GTGATAACAA GTACAAGGAG 
AAGTATACCA ACCACATGAA AGGGCACTAT GAAGGCTCTG AGATGGACAA GAATGCTGTA 
CACGCTATGA ACGTCAGAAA GCTGGCAAGC AATATTGCCT ACAAGCAGGA GTATGAGCAG 
GAGAAAACTG ACCATGTAGA ATACAACTAC CCAGCCACCA TCACACCAGG CTACCAGAGT 
CAGAGGAAAC TGGATCCACT CAAGGACAAG AACTACAGAC AGCACATTGA CCAAGTCAAG 
TACAGCTCGG TGACAGATAC CCCTGACATC GTCCAAGCCA GGATCAACGC TCAACAACTC 
AGCAATGTTA ACTACAGACA ACACATTGAC CAAGTCAAGT ACAGCTCGGT GACAGATACC 
CCTCAAATCG TCCAGGCCAG GATCAACGCT CAACAACTCA GCAATATAAA ATACAAGGAG 
GACTGGGAGA AGACCAAGTC CAAAACATGT GACATCGGCC TGGACACGCT GACCGTCAAG 
GCTGCCAAAG CATCTCGGGA CCTGGCCAGT GATATTAAGT ACAAAGAGTC ATTCATGAAA 
AATAAAGATA AGACAAGCAA TGTCAGCGAC TCCAAGACCC TGCACTCTCT TCAGGCGTCC 
AAGATGACCA GTGAGATTGA GTACAAGAAG AACTCCAAGG AGAACCAGTC CCAGTTCAGC 
TTGTCCATGG ACATGGTCAA CCTGAGCCAT GCTAAAAAAG CCCAGGCCCT TGCCAGCGAC 
CTGGACTACA GGACGAAGCT CCACCACTAC ACCATCCTAT CCGACGACAT CCAAGTACAG 
CAGGCCAAAA AGGCCTATAA CCTGCAGAGC GAGAATCAAT ACCGTTCAGA TCTGACCTGG 
ATGAAGGGAG TTGGCTGGGA GGCTGATGGA TCTCTGAATG TGACCCAAGC CAAGAAGGCT 
GGAGAGCTTC TCAGTGACAA AAAGTACCGT CAGAAAGTGG ACGACGTCAA GTTCACCCAG 
GTGGCCGATA CTCCCTCTAT CAAACACGCC AAGAAGAGCC AGGAACTGCA GAGCAAGCTA 
ACGTATAAGG CGGGCAACGA ACAGATGATG CACCAGTACA CCATGGATAA AGATGAGCCA 
CTCTTCAGGC AGGCCAAAGC CAATGCAGAC CTCCTCAGTG GGAAAGTGTA TAAGAGTAGC 
TGGGAGCAAC AGCGAGAAAA GGGCTTTGAG TTGCGCATGG ACTCCTTGTC CATCCTCACA 
GCTAAGGCTA AGAGAGACCT GGCCAGTAAC GTGAAATACA AGGAGAAGTA CGAGCAGACT 
AAGGGGAAGA TGATTGGGGT GAAGATGGTC ACTGACGACT CTCAGATGGC GCACTCGGCT 
CTGGCCACCC GGTTGCAGAG TGATCGCCAC TACAGAAAGA AGTACGAGGA CACTAAAACC 
AAGCATGGTG CCTCTTTGGA CATGTTGACC ATGACCCACG CCAAGAAGGC CCAGGATCTG 
GCAACTGAGA CCAACTACAG GACCTTCCTG CACAAGTACA CAACCCTGCC CGGCGACATG 
AAGGTGGCCT GGGCCAAGAA GGCCTACGGA CTACTGAGTG ATAAACAGTA CAGGTCGGAT 
CGGAACTGGA TGAAGGGCGT GGGCTGGGTG GCAGCAGATT TCCTGGATGT GAAACAAGCA 
AAGAAGGCTG GCGAGCTCGT TAGCGAGAAA AAGTACCGTC AACATGTCAG CGATCTGAAG 
TTCACCAGCG TGGAGGACAC TCCCGAGATG GTCCAGGCCA AACTCAGCAA CAAACTGGCC 
CTAGACAGGC TGTACAGGGA AAAGGGGGAA CATATGAAGC ACAACTACAC ACTCTCCGGA 
GATCTGCCTG AACACGTGCA GGCCAAGCTC AATGCCATGA ACATCAGTGA GACCCGCTAT 
AAGGAGTCGT GGACTAAAAT CAGAGATGGT GGCTACAAGC TCCGTTTGGA TGCCATTCCC 
TTCCAGTCAG CCAAAGCGTC TGGGAACATA CTTAGTGATC ACAAGTACAA GGAGCAGTTT 
GAAAAGACCA AGGGGCATAT GATTGGACTG AAGGGACTGG AGAACGACAT GAACATGTCT 
CACTCTGTGA ATGCCAGTAA ACTGCAGAGT GACATCAAGT ATAAGCAGGA CTCTGTGAAT 
AAGCGCTCTC AGTACCACCT TTCTATGGAC ATGTTGGAGA TGGCCCATGC TAAAAAGGCC 
CAGACCCTGG CCAGTGAGCA GGACTACCGG CTTACACTCC ACCAGTACAC TACTCAACCC 
GATGACATGA AGGTGCAGGC CGCTAAGAGA GCCTACCAAC TACAGAGCGA GAAAGTGTAC 
CGCTCTGACC TGAACTACCT GCGTGGTGTA GCCTGGATCA GCACCGGGGC CCTCCAGATG 
GAGGGCTTCA AAAGGGCCAC AGACCTTATT AGTGAGAAAA ACTACCGTCA GCAGCCCTGC 
TCCTTCAAGC ACACATCTGT CGCAGACTCA CCAGACATTG TCCATGCCAA ACTCAGCAAT 
AAGCTTACCA ACGAGCGCCT GTACAAGATG AAAGGGGAGA ATGACAGGCA TAACTACACA 
ATGACATTGG AGAGACCAGA GATAACACAG GCTAAGATCA ACGCGGCTAA CTTCAGCGAG 
ACCAAGTACA GGGAGTCATG GCACACTCTA AGGGCTCAGG GCTACAAACT GACCATGCAG 
GACATCCCCT TCCAGGCTGC CAAAACCTCA ATGGATTTAG CCAGCGACGT CCAATACAAG 
CACCACCACC TGCTGGATAA GGGCAAGCAC ATCGGCGCCC GCAGCATTCT GGATGACCCG 
CGCATGCTAC ACTGCATGCA GATGGGCCGG CTGACGAGTG AGCAGGAGTA CCGCAAAGAC 
GCACTTTCCA CCAGCGGCCA GTACCACCTC AATCCAGATA TGATCAATTT AGTGACAGCC 
AAGAACGCCC AAGCTCTGGC CAGCGATCAG GACTACAGGA AGAGGCTCCA TGCGTACACT 
GTCCTCCCTG ACGATATTAA AGTCCAATGG GCCAAGAAGG CCTATAACCT GCAGAGCGAG 
AAACTGTACA AGTCGGATCT GAACTTCATG AAGGGCGTGG CCTGGGATGG TGTGGGTGCG 
CCTCAAATGG AGTTGGCAAA GAAAGCCGGA GAACTCACCA GTAATAAGAA ATATCGTCAA 
CTGCCAGACA GTCTGAAGTT CACCCAAGTG GCGGACTCTC CAGACATTGT TCATGCTAAG 
AACAGCTACC TCCAGTGCAG TGAGAGGCTG TATAAGTCTG GAGACTCGGA CGCCATGCAC 
AAATACACCT TACATGCCGA CGACCCAGAC TTCATCAGAG CAAGGATCAA CGCCCAGCAG 
ATTAGCAATA AAGCCTATAA AGCGTCTGGG GATGAGATGA GGTCGGCAGG CTACGACTTG 
AGGCTGGATG CTATTCCCTT CCAGACGGCC AAGGCTTCCA CGAATATCGC CAGTGATTTC 
CGCTACAAGG AAGCCTTTGT GAAGGAGAAG GGTCAGCAGG TGGGGCTGCT CAGTGTCGAA 
GATGACCCAA AGATGGTGCA CTCCCTGGCA GCCAGCAAGC TCCAGAGCTG CCTGGGGTAC 
AAGAGGCAGG CTCAAGAGAC GCGCTCCCAG TTCAAGATGC ATGCCAACCA GCCTGTGTTC 
CAGCAGGCCA AGAAGAGCCA AGCTCTGGCC AGCAACCAGA CCTATCACCA GAGGCTCCAT 
GACTACACCT TTGACCCCCA GCAGCTCAAC GTCCAACACG CCAAGCAGGC CTACAAGCTG 
CAGAGTGACG TGAACTACAA GTCAGATCTG AACTGGATCA GGGGTGTGGG CTGGACCCCC 
CCTGGCTCCC ATAAGGTGGA GATGGCCCGG CGGGCTGCAG AACTGGGCCT GACGGAGAGC 
GTGAGCACTG AGGAGGCCAT TGCCCAGTAC CAACACATGA TGATGATGCA GCAGATGGAG 
GAGCAGCAGC AGCTGTCAGA ACCTGTTGAG ACCAGCAAAG AGTTCCAGCA GTCGGGAGTC 
AATCTAGACG CAATGGAGAT CCTCCACGTC AAGAGGAAGA AGACCATCCA AACGGTGAAG 
AAGTCCAATT CCTCCTCCAT GGATAGCAGC GTCAAGAAGT TAATGATGTC TTCCAACTCA 
GCCATAGAGT CAATGATGGC TTCCACCTCT GTCTCAGCCA TTGAGAACAC AGTCAAGATG 
TCCACAACGA GTGAGGCATC GGCCATAGAA AATGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021431134.1
CDS	198..5396
Translation	MQSCARCGFVVYPAEKINCIDQNWHKACFHCDVCKMVLTAKNFVSHKKRPYCSVHNPRNNMFTSVYETPINAKKQAIASSEQDYRAKFEEQQTYSYSGTITNQEIVSVTQAQKTISDVKYTEEYEQSKGKGSLSTMITPGYQISKKPNTLPSSYVYTEEYEQQLGKGSFPAHLTPGYQVSKKATEMASGVKYRQMYEQEMKGKATSDAGAAEFASARENAGNFSQIAYTEEYEQQRGKGSFPAMITPAYQLAKKAQAHASDLKYKKDLNKMKGSSHFHNLTSEDNLALKSARKINKIVSEVEYKKDLENTKGHSINYCETPQYKNASKAAKFSSDNKYKEKYTNHMKGHYEGSEMDKNAVHAMNVRKLASNIAYKQEYEQEKTDHVEYNYPATITPGYQSQRKLDPLKDKNYRQHIDQVKYSSVTDTPDIVQARINAQQLSNVNYRQHIDQVKYSSVTDTPQIVQARINAQQLSNIKYKEDWEKTKSKTCDIGLDTLTVKAAKASRDLASDIKYKESFMKNKDKTSNVSDSKTLHSLQASKMTSEIEYKKNSKENQSQFSLSMDMVNLSHAKKAQALASDLDYRTKLHHYTILSDDIQVQQAKKAYNLQSENQYRSDLTWMKGVGWEADGSLNVTQAKKAGELLSDKKYRQKVDDVKFTQVADTPSIKHAKKSQELQSKLTYKAGNEQMMHQYTMDKDEPLFRQAKANADLLSGKVYKSSWEQQREKGFELRMDSLSILTAKAKRDLASNVKYKEKYEQTKGKMIGVKMVTDDSQMAHSALATRLQSDRHYRKKYEDTKTKHGASLDMLTMTHAKKAQDLATETNYRTFLHKYTTLPGDMKVAWAKKAYGLLSDKQYRSDRNWMKGVGWVAADFLDVKQAKKAGELVSEKKYRQHVSDLKFTSVEDTPEMVQAKLSNKLALDRLYREKGEHMKHNYTLSGDLPEHVQAKLNAMNISETRYKESWTKIRDGGYKLRLDAIPFQSAKASGNILSDHKYKEQFEKTKGHMIGLKGLENDMNMSHSVNASKLQSDIKYKQDSVNKRSQYHLSMDMLEMAHAKKAQTLASEQDYRLTLHQYTTQPDDMKVQAAKRAYQLQSEKVYRSDLNYLRGVAWISTGALQMEGFKRATDLISEKNYRQQPCSFKHTSVADSPDIVHAKLSNKLTNERLYKMKGENDRHNYTMTLERPEITQAKINAANFSETKYRESWHTLRAQGYKLTMQDIPFQAAKTSMDLASDVQYKHHHLLDKGKHIGARSILDDPRMLHCMQMGRLTSEQEYRKDALSTSGQYHLNPDMINLVTAKNAQALASDQDYRKRLHAYTVLPDDIKVQWAKKAYNLQSEKLYKSDLNFMKGVAWDGVGAPQMELAKKAGELTSNKKYRQLPDSLKFTQVADSPDIVHAKNSYLQCSERLYKSGDSDAMHKYTLHADDPDFIRARINAQQISNKAYKASGDEMRSAGYDLRLDAIPFQTAKASTNIASDFRYKEAFVKEKGQQVGLLSVEDDPKMVHSLAASKLQSCLGYKRQAQETRSQFKMHANQPVFQQAKKSQALASNQTYHQRLHDYTFDPQQLNVQHAKQAYKLQSDVNYKSDLNWIRGVGWTPPGSHKVEMARRAAELGLTESVSTEEAIAQYQHMMMMQQMEEQQQLSEPVETSKEFQQSGVNLDAMEILHVKRKKTIQTVKKSNSSSMDSSVKKLMMSSNSAIESMMASTSVSAIENTVKMSTTSEASAIENA

Target ORF information:

RefSeq Version	XM_021575459.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021575459.1

ORF Insert Sequence:

ATGCAGTCCT GCGCCAGGTG TGGGTTTGTG GTGTACCCAG CGGAGAAGAT CAATTGTATT 
GATCAGAACT GGCACAAAGC ATGCTTTCAC TGCGACGTCT GTAAAATGGT GCTCACAGCC 
AAGAACTTTG TTAGCCACAA GAAGCGGCCA TACTGCTCTG TACACAATCC AAGAAACAAT 
ATGTTCACCA GTGTCTATGA GACCCCCATC AATGCCAAGA AGCAAGCCAT TGCCAGCAGT 
GAGCAGGACT ATCGGGCTAA GTTTGAGGAG CAGCAGACCT ACTCGTACTC AGGCACCATC 
ACCAACCAGG AGATTGTGAG CGTGACACAG GCCCAAAAGA CCATCAGTGA TGTCAAATAC 
ACTGAGGAAT ATGAGCAGAG TAAAGGAAAA GGCAGCTTAT CCACCATGAT CACACCAGGA 
TACCAGATTT CCAAGAAACC AAATACCTTG CCCAGCAGCT ATGTCTACAC TGAAGAGTAT 
GAACAGCAGT TGGGTAAAGG CAGTTTCCCC GCACATCTCA CTCCTGGCTA CCAAGTTTCA 
AAGAAAGCCA CAGAGATGGC CAGTGGTGTG AAGTACCGTC AGATGTATGA GCAGGAGATG 
AAGGGAAAGG CCACCTCAGA TGCTGGGGCA GCAGAATTCG CTTCTGCCAG AGAAAATGCT 
GGGAACTTCA GCCAGATTGC CTACACTGAA GAGTACGAGC AGCAGAGAGG GAAGGGCAGT 
TTCCCAGCCA TGATCACTCC TGCCTACCAG CTTGCCAAGA AAGCCCAGGC CCACGCCAGT 
GATCTGAAAT ACAAGAAGGA CCTGAATAAG ATGAAGGGCT CATCCCACTT CCACAACCTG 
ACCAGTGAGG ACAACCTGGC CCTGAAGAGC GCCCGCAAAA TCAACAAGAT CGTCAGCGAG 
GTTGAGTACA AGAAGGACCT TGAGAACACT AAAGGCCACA GCATCAACTA CTGTGAGACG 
CCCCAGTACA AGAATGCATC CAAGGCTGCC AAGTTCAGCA GTGATAACAA GTACAAGGAG 
AAGTATACCA ACCACATGAA AGGGCACTAT GAAGGCTCTG AGATGGACAA GAATGCTGTA 
CACGCTATGA ACGTCAGAAA GCTGGCAAGC AATATTGCCT ACAAGCAGGA GTATGAGCAG 
GAGAAAACTG ACCATGTAGA ATACAACTAC CCAGCCACCA TCACACCAGG CTACCAGAGT 
CAGAGGAAAC TGGATCCACT CAAGGACAAG AACTACAGAC AGCACATTGA CCAAGTCAAG 
TACAGCTCGG TGACAGATAC CCCTGACATC GTCCAAGCCA GGATCAACGC TCAACAACTC 
AGCAATGTTA ACTACAGACA ACACATTGAC CAAGTCAAGT ACAGCTCGGT GACAGATACC 
CCTCAAATCG TCCAGGCCAG GATCAACGCT CAACAACTCA GCAATATAAA ATACAAGGAG 
GACTGGGAGA AGACCAAGTC CAAAACATGT GACATCGGCC TGGACACGCT GACCGTCAAG 
GCTGCCAAAG CATCTCGGGA CCTGGCCAGT GATATTAAGT ACAAAGAGTC ATTCATGAAA 
AATAAAGATA AGACAAGCAA TGTCAGCGAC TCCAAGACCC TGCACTCTCT TCAGGCGTCC 
AAGATGACCA GTGAGATTGA GTACAAGAAG AACTCCAAGG AGAACCAGTC CCAGTTCAGC 
TTGTCCATGG ACATGGTCAA CCTGAGCCAT GCTAAAAAAG CCCAGGCCCT TGCCAGCGAC 
CTGGACTACA GGACGAAGCT CCACCACTAC ACCATCCTAT CCGACGACAT CCAAGTACAG 
CAGGCCAAAA AGGCCTATAA CCTGCAGAGC GAGAATCAAT ACCGTTCAGA TCTGACCTGG 
ATGAAGGGAG TTGGCTGGGA GGCTGATGGA TCTCTGAATG TGACCCAAGC CAAGAAGGCT 
GGAGAGCTTC TCAGTGACAA AAAGTACCGT CAGAAAGTGG ACGACGTCAA GTTCACCCAG 
GTGGCCGATA CTCCCTCTAT CAAACACGCC AAGAAGAGCC AGGAACTGCA GAGCAAGCTA 
ACGTATAAGG CGGGCAACGA ACAGATGATG CACCAGTACA CCATGGATAA AGATGAGCCA 
CTCTTCAGGC AGGCCAAAGC CAATGCAGAC CTCCTCAGTG GGAAAGTGTA TAAGAGTAGC 
TGGGAGCAAC AGCGAGAAAA GGGCTTTGAG TTGCGCATGG ACTCCTTGTC CATCCTCACA 
GCTAAGGCTA AGAGAGACCT GGCCAGTAAC GTGAAATACA AGGAGAAGTA CGAGCAGACT 
AAGGGGAAGA TGATTGGGGT GAAGATGGTC ACTGACGACT CTCAGATGGC GCACTCGGCT 
CTGGCCACCC GGTTGCAGAG TGATCGCCAC TACAGAAAGA AGTACGAGGA CACTAAAACC 
AAGCATGGTG CCTCTTTGGA CATGTTGACC ATGACCCACG CCAAGAAGGC CCAGGATCTG 
GCAACTGAGA CCAACTACAG GACCTTCCTG CACAAGTACA CAACCCTGCC CGGCGACATG 
AAGGTGGCCT GGGCCAAGAA GGCCTACGGA CTACTGAGTG ATAAACAGTA CAGGTCGGAT 
CGGAACTGGA TGAAGGGCGT GGGCTGGGTG GCAGCAGATT TCCTGGATGT GAAACAAGCA 
AAGAAGGCTG GCGAGCTCGT TAGCGAGAAA AAGTACCGTC AACATGTCAG CGATCTGAAG 
TTCACCAGCG TGGAGGACAC TCCCGAGATG GTCCAGGCCA AACTCAGCAA CAAACTGGCC 
CTAGACAGGC TGTACAGGGA AAAGGGGGAA CATATGAAGC ACAACTACAC ACTCTCCGGA 
GATCTGCCTG AACACGTGCA GGCCAAGCTC AATGCCATGA ACATCAGTGA GACCCGCTAT 
AAGGAGTCGT GGACTAAAAT CAGAGATGGT GGCTACAAGC TCCGTTTGGA TGCCATTCCC 
TTCCAGTCAG CCAAAGCGTC TGGGAACATA CTTAGTGATC ACAAGTACAA GGAGCAGTTT 
GAAAAGACCA AGGGGCATAT GATTGGACTG AAGGGACTGG AGAACGACAT GAACATGTCT 
CACTCTGTGA ATGCCAGTAA ACTGCAGAGT GACATCAAGT ATAAGCAGGA CTCTGTGAAT 
AAGCGCTCTC AGTACCACCT TTCTATGGAC ATGTTGGAGA TGGCCCATGC TAAAAAGGCC 
CAGACCCTGG CCAGTGAGCA GGACTACCGG CTTACACTCC ACCAGTACAC TACTCAACCC 
GATGACATGA AGGTGCAGGC CGCTAAGAGA GCCTACCAAC TACAGAGCGA GAAAGTGTAC 
CGCTCTGACC TGAACTACCT GCGTGGTGTA GCCTGGATCA GCACCGGGGC CCTCCAGATG 
GAGGGCTTCA AAAGGGCCAC AGACCTTATT AGTGAGAAAA ACTACCGTCA GCAGCCCTGC 
TCCTTCAAGC ACACATCTGT CGCAGACTCA CCAGACATTG TCCATGCCAA ACTCAGCAAT 
AAGCTTACCA ACGAGCGCCT GTACAAGATG AAAGGGGAGA ATGACAGGCA TAACTACACA 
ATGACATTGG AGAGACCAGA GATAACACAG GCTAAGATCA ACGCGGCTAA CTTCAGCGAG 
ACCAAGTACA GGGAGTCATG GCACACTCTA AGGGCTCAGG GCTACAAACT GACCATGCAG 
GACATCCCCT TCCAGGCTGC CAAAACCTCA ATGGATTTAG CCAGCGACGT CCAATACAAG 
CACCACCACC TGCTGGATAA GGGCAAGCAC ATCGGCGCCC GCAGCATTCT GGATGACCCG 
CGCATGCTAC ACTGCATGCA GATGGGCCGG CTGACGAGTG AGCAGGAGTA CCGCAAAGAC 
GCACTTTCCA CCAGCGGCCA GTACCACCTC AATCCAGATA TGATCAATTT AGTGACAGCC 
AAGAACGCCC AAGCTCTGGC CAGCGATCAG GACTACAGGA AGAGGCTCCA TGCGTACACT 
GTCCTCCCTG ACGATATTAA AGTCCAATGG GCCAAGAAGG CCTATAACCT GCAGAGCGAG 
AAACTGTACA AGTCGGATCT GAACTTCATG AAGGGCGTGG CCTGGGATGG TGTGGGTGCG 
CCTCAAATGG AGTTGGCAAA GAAAGCCGGA GAACTCACCA GTAATAAGAA ATATCGTCAA 
CTGCCAGACA GTCTGAAGTT CACCCAAGTG GCGGACTCTC CAGACATTGT TCATGCTAAG 
AACAGCTACC TCCAGTGCAG TGAGAGGCTG TATAAGTCTG GAGACTCGGA CGCCATGCAC 
AAATACACCT TACATGCCGA CGACCCAGAC TTCATCAGAG CAAGGATCAA CGCCCAGCAG 
ATTAGCAATA AAGCCTATAA AGCGTCTGGG GATGAGATGA GGTCGGCAGG CTACGACTTG 
AGGCTGGATG CTATTCCCTT CCAGACGGCC AAGGCTTCCA CGAATATCGC CAGTGATTTC 
CGCTACAAGG AAGCCTTTGT GAAGGAGAAG GGTCAGCAGG TGGGGCTGCT CAGTGTCGAA 
GATGACCCAA AGATGGTGCA CTCCCTGGCA GCCAGCAAGC TCCAGAGCTG CCTGGGGTAC 
AAGAGGCAGG CTCAAGAGAC GCGCTCCCAG TTCAAGATGC ATGCCAACCA GCCTGTGTTC 
CAGCAGGCCA AGAAGAGCCA AGCTCTGGCC AGCAACCAGA CCTATCACCA GAGGCTCCAT 
GACTACACCT TTGACCCCCA GCAGCTCAAC GTCCAACACG CCAAGCAGGC CTACAAGCTG 
CAGAGTGACG TGAACTACAA GTCAGATCTG AACTGGATCA GGGGTGTGGG CTGGACCCCC 
CCTGGCTCCC ATAAGGTGGA GATGGCCCGG CGGGCTGCAG AACTGGGCCT GACGGAGAGC 
GTGAGCACTG AGGAGGCCAT TGCCCAGTAC CAACACATGA TGATGATGCA GCAGATGGAG 
GAGCAGCAGC AGCTGTCAGA ACCTGTTGAG ACCAGCAAAG AGTTCCAGCA GTCGGGAGTC 
AATCTAGACG CAATGGAGAT CCTCCACGTC AAGAGGAAGA AGACCATCCA AACGGTGAAG 
AAGTCCAATT CCTCCTCCAT GGATAGCAGC GTCAAGAAGT TAATGATGTC TTCCAACTCA 
GCCATAGAGT CAATGATGGC TTCCACCTCT GTCTCAGCCA TTGAGAACAC AGTCAAGATG 
TCCACAACGA GTGAGGCATC GGCCATAGAA AATGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrap ( XM_021575460.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021431135.1 Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X4, mRNA.

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CloneID	OOf25631
Clone ID Related Accession (Same CDS sequence)	XM_021575460.1
Accession Version	XM_021575460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	nebulin-related-anchoring protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT GCGCCAGGTG TGGGTTTGTG GTGTACCCAG CGGAGAAGAT CAATTGTATT 
GATCAGAACT GGCACAAAGC ATGCTTTCAC TGCGACGTCT GTAAAATGGT GCTCACAGCC 
AAGAACTTTG TTAGCCACAA GAAGCGGCCA TACTGCTCTG TACACAATCC AAGAAACAAT 
ATGTTCACCA GTGTCTATGA GACCCCCATC AATGCCAAGA AGCAAGCCAT TGCCAGCAGT 
GAGCAGGACT ATCGGGCTAA GTTTGAGGAG CAGCAGACCT ACTCGTACTC AGGCACCATC 
ACCAACCAGG AGATTGTGAG CGTGACACAG GCCCAAAAGA CCATCAGTGA TGTCAAATAC 
ACTGAGGAAT ATGAGCAGAG TAAAGGAAAA GGCAGCTTAT CCACCATGAT CACACCAGGA 
TACCAGATTT CCAAGAAACC AAATACCTTG CCCAGCAGCG CGGAGTACAA TAAAGGAGAG 
GAGAAAGTGT CAAAATATTC CTCTGTAGCC AAAACCCCTG AAGTGCTTCT GGCCAAAAGC 
CAAGGACAGA TCCATTATGT CTACACTGAA GAGTATGAAC AGCAGTTGGG TAAAGGCAGT 
TTCCCCGCAC ATCTCACTCC TGGCTACCAA GTTTCAAAGA AAGCCACAGA GATGGCCAGT 
GGTGTGAAGT ACCGTCAGAT GTATGAGCAG GAGATGAAGG GAAAGGCCAC CTCAGATGCT 
GGGGCAGCAG AATTCGCTTC TGCCAGAGAA AATGCTGGGA ACTTCAGCCA GATTGCCTAC 
ACTGAAGAGT ACGAGCAGCA GAGAGGGAAG GGCAGTTTCC CAGCCATGAT CACTCCTGCC 
TACCAGCTTG CCAAGAAAGC CCAGGCCCAC GCCAGTGATC TGAAATACAA GAAGGACCTG 
AATAAGATGA AGGGCTCATC CCACTTCCAC AACCTGACCA GTGAGGACAA CCTGGCCCTG 
AAGAGCGCCC GCAAAATCAA CAAGATCGTC AGCGAGGTTG AGTACAAGAA GGACCTTGAG 
AACACTAAAG GCCACAGCAT CAACTACTGT GAGACGCCCC AGTACAAGAA TGCATCCAAG 
GCTGCCAAGT TCAGCAGTGA TAACAAGTAC AAGGAGAAGT ATACCAACCA CATGAAAGGG 
CACTATGAAG GCTCTGAGAT GGACAAGAAT GCTGTACACG CTATGAACGT CAGAAAGCTG 
GCAAGCAATA TTGCCTACAA GCAGGAGTAT GAGCAGGAGA AAACTGACCA TGTAGAATAC 
AACTACCCAG CCACCATCAC ACCAGGCTAC CAGAGTCAGA GGAAACTGGA TCCACTCAAG 
GACAAGAACT ACAGACAGCA CATTGACCAA GTCAAGTACA GCTCGGTGAC AGATACCCCT 
GACATCGTCC AAGCCAGGAT CAACGCTCAA CAACTCAGCA ATATAAAATA CAAGGAGGAC 
TGGGAGAAGA CCAAGTCCAA AACATGTGAC ATCGGCCTGG ACACGCTGAC CGTCAAGGCT 
GCCAAAGCAT CTCGGGACCT GGCCAGTGAT ATTAAGTACA AAGAGTCATT CATGAAAAAT 
AAAGATAAGA CAAGCAATGT CAGCGACTCC AAGACCCTGC ACTCTCTTCA GGCGTCCAAG 
ATGACCAGTG AGATTGAGTA CAAGAAGAAC TCCAAGGAGA ACCAGTCCCA GTTCAGCTTG 
TCCATGGACA TGGTCAACCT GAGCCATGCT AAAAAAGCCC AGGCCCTTGC CAGCGACCTG 
GACTACAGGA CGAAGCTCCA CCACTACACC ATCCTATCCG ACGACATCCA AGTACAGCAG 
GCCAAAAAGG CCTATAACCT GCAGAGCGAG AATCAATACC GTTCAGATCT GACCTGGATG 
AAGGGAGTTG GCTGGGAGGC TGATGGATCT CTGAATGTGA CCCAAGCCAA GAAGGCTGGA 
GAGCTTCTCA GTGACAAAAA GTACCGTCAG AAAGTGGACG ACGTCAAGTT CACCCAGGTG 
GCCGATACTC CCTCTATCAA ACACGCCAAG AAGAGCCAGG AACTGCAGAG CAAGCTAACG 
TATAAGGCGG GCAACGAACA GATGATGCAC CAGTACACCA TGGATAAAGA TGAGCCACTC 
TTCAGGCAGG CCAAAGCCAA TGCAGACCTC CTCAGTGGGA AAGTGTATAA GAGTAGCTGG 
GAGCAACAGC GAGAAAAGGG CTTTGAGTTG CGCATGGACT CCTTGTCCAT CCTCACAGCT 
AAGGCTAAGA GAGACCTGGC CAGTAACGTG AAATACAAGG AGAAGTACGA GCAGACTAAG 
GGGAAGATGA TTGGGGTGAA GATGGTCACT GACGACTCTC AGATGGCGCA CTCGGCTCTG 
GCCACCCGGT TGCAGAGTGA TCGCCACTAC AGAAAGAAGT ACGAGGACAC TAAAACCAAG 
CATGGTGCCT CTTTGGACAT GTTGACCATG ACCCACGCCA AGAAGGCCCA GGATCTGGCA 
ACTGAGACCA ACTACAGGAC CTTCCTGCAC AAGTACACAA CCCTGCCCGG CGACATGAAG 
GTGGCCTGGG CCAAGAAGGC CTACGGACTA CTGAGTGATA AACAGTACAG GTCGGATCGG 
AACTGGATGA AGGGCGTGGG CTGGGTGGCA GCAGATTTCC TGGATGTGAA ACAAGCAAAG 
AAGGCTGGCG AGCTCGTTAG CGAGAAAAAG TACCGTCAAC ATGTCAGCGA TCTGAAGTTC 
ACCAGCGTGG AGGACACTCC CGAGATGGTC CAGGCCAAAC TCAGCAACAA ACTGGCCCTA 
GACAGGCTGT ACAGGGAAAA GGGGGAACAT ATGAAGCACA ACTACACACT CTCCGGAGAT 
CTGCCTGAAC ACGTGCAGGC CAAGCTCAAT GCCATGAACA TCAGTGAGAC CCGCTATAAG 
GAGTCGTGGA CTAAAATCAG AGATGGTGGC TACAAGCTCC GTTTGGATGC CATTCCCTTC 
CAGTCAGCCA AAGCGTCTGG GAACATACTT AGTGATCACA AGTACAAGGA GCAGTTTGAA 
AAGACCAAGG GGCATATGAT TGGACTGAAG GGACTGGAGA ACGACATGAA CATGTCTCAC 
TCTGTGAATG CCAGTAAACT GCAGAGTGAC ATCAAGTATA AGCAGGACTC TGTGAATAAG 
CGCTCTCAGT ACCACCTTTC TATGGACATG TTGGAGATGG CCCATGCTAA AAAGGCCCAG 
ACCCTGGCCA GTGAGCAGGA CTACCGGCTT ACACTCCACC AGTACACTAC TCAACCCGAT 
GACATGAAGG TGCAGGCCGC TAAGAGAGCC TACCAACTAC AGAGCGAGAA AGTGTACCGC 
TCTGACCTGA ACTACCTGCG TGGTGTAGCC TGGATCAGCA CCGGGGCCCT CCAGATGGAG 
GGCTTCAAAA GGGCCACAGA CCTTATTAGT GAGAAAAACT ACCGTCAGCA GCCCTGCTCC 
TTCAAGCACA CATCTGTCGC AGACTCACCA GACATTGTCC ATGCCAAACT CAGCAATAAG 
CTTACCAACG AGCGCCTGTA CAAGATGAAA GGGGAGAATG ACAGGCATAA CTACACAATG 
ACATTGGAGA GACCAGAGAT AACACAGGCT AAGATCAACG CGGCTAACTT CAGCGAGACC 
AAGTACAGGG AGTCATGGCA CACTCTAAGG GCTCAGGGCT ACAAACTGAC CATGCAGGAC 
ATCCCCTTCC AGGCTGCCAA AACCTCAATG GATTTAGCCA GCGACGTCCA ATACAAGCAC 
CACCACCTGC TGGATAAGGG CAAGCACATC GGCGCCCGCA GCATTCTGGA TGACCCGCGC 
ATGCTACACT GCATGCAGAT GGGCCGGCTG ACGAGTGAGC AGGAGTACCG CAAAGACGCA 
CTTTCCACCA GCGGCCAGTA CCACCTCAAT CCAGATATGA TCAATTTAGT GACAGCCAAG 
AACGCCCAAG CTCTGGCCAG CGATCAGGAC TACAGGAAGA GGCTCCATGC GTACACTGTC 
CTCCCTGACG ATATTAAAGT CCAATGGGCC AAGAAGGCCT ATAACCTGCA GAGCGAGAAA 
CTGTACAAGT CGGATCTGAA CTTCATGAAG GGCGTGGCCT GGGATGGTGT GGGTGCGCCT 
CAAATGGAGT TGGCAAAGAA AGCCGGAGAA CTCACCAGTA ATAAGAAATA TCGTCAACTG 
CCAGACAGTC TGAAGTTCAC CCAAGTGGCG GACTCTCCAG ACATTGTTCA TGCTAAGAAC 
AGCTACCTCC AGTGCAGTGA GAGGCTGTAT AAGTCTGGAG ACTCGGACGC CATGCACAAA 
TACACCTTAC ATGCCGACGA CCCAGACTTC ATCAGAGCAA GGATCAACGC CCAGCAGATT 
AGCAATAAAG CCTATAAAGC GTCTGGGGAT GAGATGAGGT CGGCAGGCTA CGACTTGAGG 
CTGGATGCTA TTCCCTTCCA GACGGCCAAG GCTTCCACGA ATATCGCCAG TGATTTCCGC 
TACAAGGAAG CCTTTGTGAA GGAGAAGGGT CAGCAGGTGG GGCTGCTCAG TGTCGAAGAT 
GACCCAAAGA TGGTGCACTC CCTGGCAGCC AGCAAGCTCC AGAGCTGCCT GGGGTACAAG 
AGGCAGGCTC AAGAGACGCG CTCCCAGTTC AAGATGCATG CCAACCAGCC TGTGTTCCAG 
CAGGCCAAGA AGAGCCAAGC TCTGGCCAGC AACCAGACCT ATCACCAGAG GCTCCATGAC 
TACACCTTTG ACCCCCAGCA GCTCAACGTC CAACACGCCA AGCAGGCCTA CAAGCTGCAG 
AGTGACGTGA ACTACAAGTC AGATCTGAAC TGGATCAGGG GTGTGGGCTG GACCCCCCCT 
GGCTCCCATA AGGTGGAGAT GGCCCGGCGG GCTGCAGAAC TGGGCCTGAC GGAGAGCGTG 
AGCACTGAGG AGGCCATTGC CCAGTACCAA CACATGATGA TGATGCAGCA GATGGAGGAG 
CAGCAGCAGC TGTCAGAACC TGTTGAGACC AGCAAAGAGT TCCAGCAGTC GGGAGTCAAT 
CTAGACGCAA TGGAGATCCT CCACGTCAAG AGGAAGAAGA CCATCCAAAC GGTGAAGAAG 
TCCAATTCCT CCTCCATGGA TAGCAGCGTC AAGAAGTTAA TGATGTCTTC CAACTCAGCC 
ATAGAGTCAA TGATGGCTTC CACCTCTGTC TCAGCCATTG AGAACACAGT CAAGATGTCC 
ACAACGAGTG AGGCATCGGC CATAGAAAAT GCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021431135.1
CDS	198..5393
Translation	MQSCARCGFVVYPAEKINCIDQNWHKACFHCDVCKMVLTAKNFVSHKKRPYCSVHNPRNNMFTSVYETPINAKKQAIASSEQDYRAKFEEQQTYSYSGTITNQEIVSVTQAQKTISDVKYTEEYEQSKGKGSLSTMITPGYQISKKPNTLPSSAEYNKGEEKVSKYSSVAKTPEVLLAKSQGQIHYVYTEEYEQQLGKGSFPAHLTPGYQVSKKATEMASGVKYRQMYEQEMKGKATSDAGAAEFASARENAGNFSQIAYTEEYEQQRGKGSFPAMITPAYQLAKKAQAHASDLKYKKDLNKMKGSSHFHNLTSEDNLALKSARKINKIVSEVEYKKDLENTKGHSINYCETPQYKNASKAAKFSSDNKYKEKYTNHMKGHYEGSEMDKNAVHAMNVRKLASNIAYKQEYEQEKTDHVEYNYPATITPGYQSQRKLDPLKDKNYRQHIDQVKYSSVTDTPDIVQARINAQQLSNIKYKEDWEKTKSKTCDIGLDTLTVKAAKASRDLASDIKYKESFMKNKDKTSNVSDSKTLHSLQASKMTSEIEYKKNSKENQSQFSLSMDMVNLSHAKKAQALASDLDYRTKLHHYTILSDDIQVQQAKKAYNLQSENQYRSDLTWMKGVGWEADGSLNVTQAKKAGELLSDKKYRQKVDDVKFTQVADTPSIKHAKKSQELQSKLTYKAGNEQMMHQYTMDKDEPLFRQAKANADLLSGKVYKSSWEQQREKGFELRMDSLSILTAKAKRDLASNVKYKEKYEQTKGKMIGVKMVTDDSQMAHSALATRLQSDRHYRKKYEDTKTKHGASLDMLTMTHAKKAQDLATETNYRTFLHKYTTLPGDMKVAWAKKAYGLLSDKQYRSDRNWMKGVGWVAADFLDVKQAKKAGELVSEKKYRQHVSDLKFTSVEDTPEMVQAKLSNKLALDRLYREKGEHMKHNYTLSGDLPEHVQAKLNAMNISETRYKESWTKIRDGGYKLRLDAIPFQSAKASGNILSDHKYKEQFEKTKGHMIGLKGLENDMNMSHSVNASKLQSDIKYKQDSVNKRSQYHLSMDMLEMAHAKKAQTLASEQDYRLTLHQYTTQPDDMKVQAAKRAYQLQSEKVYRSDLNYLRGVAWISTGALQMEGFKRATDLISEKNYRQQPCSFKHTSVADSPDIVHAKLSNKLTNERLYKMKGENDRHNYTMTLERPEITQAKINAANFSETKYRESWHTLRAQGYKLTMQDIPFQAAKTSMDLASDVQYKHHHLLDKGKHIGARSILDDPRMLHCMQMGRLTSEQEYRKDALSTSGQYHLNPDMINLVTAKNAQALASDQDYRKRLHAYTVLPDDIKVQWAKKAYNLQSEKLYKSDLNFMKGVAWDGVGAPQMELAKKAGELTSNKKYRQLPDSLKFTQVADSPDIVHAKNSYLQCSERLYKSGDSDAMHKYTLHADDPDFIRARINAQQISNKAYKASGDEMRSAGYDLRLDAIPFQTAKASTNIASDFRYKEAFVKEKGQQVGLLSVEDDPKMVHSLAASKLQSCLGYKRQAQETRSQFKMHANQPVFQQAKKSQALASNQTYHQRLHDYTFDPQQLNVQHAKQAYKLQSDVNYKSDLNWIRGVGWTPPGSHKVEMARRAAELGLTESVSTEEAIAQYQHMMMMQQMEEQQQLSEPVETSKEFQQSGVNLDAMEILHVKRKKTIQTVKKSNSSSMDSSVKKLMMSSNSAIESMMASTSVSAIENTVKMSTTSEASAIENA

Target ORF information:

RefSeq Version	XM_021575460.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021575460.1

ORF Insert Sequence:

ATGCAGTCCT GCGCCAGGTG TGGGTTTGTG GTGTACCCAG CGGAGAAGAT CAATTGTATT 
GATCAGAACT GGCACAAAGC ATGCTTTCAC TGCGACGTCT GTAAAATGGT GCTCACAGCC 
AAGAACTTTG TTAGCCACAA GAAGCGGCCA TACTGCTCTG TACACAATCC AAGAAACAAT 
ATGTTCACCA GTGTCTATGA GACCCCCATC AATGCCAAGA AGCAAGCCAT TGCCAGCAGT 
GAGCAGGACT ATCGGGCTAA GTTTGAGGAG CAGCAGACCT ACTCGTACTC AGGCACCATC 
ACCAACCAGG AGATTGTGAG CGTGACACAG GCCCAAAAGA CCATCAGTGA TGTCAAATAC 
ACTGAGGAAT ATGAGCAGAG TAAAGGAAAA GGCAGCTTAT CCACCATGAT CACACCAGGA 
TACCAGATTT CCAAGAAACC AAATACCTTG CCCAGCAGCG CGGAGTACAA TAAAGGAGAG 
GAGAAAGTGT CAAAATATTC CTCTGTAGCC AAAACCCCTG AAGTGCTTCT GGCCAAAAGC 
CAAGGACAGA TCCATTATGT CTACACTGAA GAGTATGAAC AGCAGTTGGG TAAAGGCAGT 
TTCCCCGCAC ATCTCACTCC TGGCTACCAA GTTTCAAAGA AAGCCACAGA GATGGCCAGT 
GGTGTGAAGT ACCGTCAGAT GTATGAGCAG GAGATGAAGG GAAAGGCCAC CTCAGATGCT 
GGGGCAGCAG AATTCGCTTC TGCCAGAGAA AATGCTGGGA ACTTCAGCCA GATTGCCTAC 
ACTGAAGAGT ACGAGCAGCA GAGAGGGAAG GGCAGTTTCC CAGCCATGAT CACTCCTGCC 
TACCAGCTTG CCAAGAAAGC CCAGGCCCAC GCCAGTGATC TGAAATACAA GAAGGACCTG 
AATAAGATGA AGGGCTCATC CCACTTCCAC AACCTGACCA GTGAGGACAA CCTGGCCCTG 
AAGAGCGCCC GCAAAATCAA CAAGATCGTC AGCGAGGTTG AGTACAAGAA GGACCTTGAG 
AACACTAAAG GCCACAGCAT CAACTACTGT GAGACGCCCC AGTACAAGAA TGCATCCAAG 
GCTGCCAAGT TCAGCAGTGA TAACAAGTAC AAGGAGAAGT ATACCAACCA CATGAAAGGG 
CACTATGAAG GCTCTGAGAT GGACAAGAAT GCTGTACACG CTATGAACGT CAGAAAGCTG 
GCAAGCAATA TTGCCTACAA GCAGGAGTAT GAGCAGGAGA AAACTGACCA TGTAGAATAC 
AACTACCCAG CCACCATCAC ACCAGGCTAC CAGAGTCAGA GGAAACTGGA TCCACTCAAG 
GACAAGAACT ACAGACAGCA CATTGACCAA GTCAAGTACA GCTCGGTGAC AGATACCCCT 
GACATCGTCC AAGCCAGGAT CAACGCTCAA CAACTCAGCA ATATAAAATA CAAGGAGGAC 
TGGGAGAAGA CCAAGTCCAA AACATGTGAC ATCGGCCTGG ACACGCTGAC CGTCAAGGCT 
GCCAAAGCAT CTCGGGACCT GGCCAGTGAT ATTAAGTACA AAGAGTCATT CATGAAAAAT 
AAAGATAAGA CAAGCAATGT CAGCGACTCC AAGACCCTGC ACTCTCTTCA GGCGTCCAAG 
ATGACCAGTG AGATTGAGTA CAAGAAGAAC TCCAAGGAGA ACCAGTCCCA GTTCAGCTTG 
TCCATGGACA TGGTCAACCT GAGCCATGCT AAAAAAGCCC AGGCCCTTGC CAGCGACCTG 
GACTACAGGA CGAAGCTCCA CCACTACACC ATCCTATCCG ACGACATCCA AGTACAGCAG 
GCCAAAAAGG CCTATAACCT GCAGAGCGAG AATCAATACC GTTCAGATCT GACCTGGATG 
AAGGGAGTTG GCTGGGAGGC TGATGGATCT CTGAATGTGA CCCAAGCCAA GAAGGCTGGA 
GAGCTTCTCA GTGACAAAAA GTACCGTCAG AAAGTGGACG ACGTCAAGTT CACCCAGGTG 
GCCGATACTC CCTCTATCAA ACACGCCAAG AAGAGCCAGG AACTGCAGAG CAAGCTAACG 
TATAAGGCGG GCAACGAACA GATGATGCAC CAGTACACCA TGGATAAAGA TGAGCCACTC 
TTCAGGCAGG CCAAAGCCAA TGCAGACCTC CTCAGTGGGA AAGTGTATAA GAGTAGCTGG 
GAGCAACAGC GAGAAAAGGG CTTTGAGTTG CGCATGGACT CCTTGTCCAT CCTCACAGCT 
AAGGCTAAGA GAGACCTGGC CAGTAACGTG AAATACAAGG AGAAGTACGA GCAGACTAAG 
GGGAAGATGA TTGGGGTGAA GATGGTCACT GACGACTCTC AGATGGCGCA CTCGGCTCTG 
GCCACCCGGT TGCAGAGTGA TCGCCACTAC AGAAAGAAGT ACGAGGACAC TAAAACCAAG 
CATGGTGCCT CTTTGGACAT GTTGACCATG ACCCACGCCA AGAAGGCCCA GGATCTGGCA 
ACTGAGACCA ACTACAGGAC CTTCCTGCAC AAGTACACAA CCCTGCCCGG CGACATGAAG 
GTGGCCTGGG CCAAGAAGGC CTACGGACTA CTGAGTGATA AACAGTACAG GTCGGATCGG 
AACTGGATGA AGGGCGTGGG CTGGGTGGCA GCAGATTTCC TGGATGTGAA ACAAGCAAAG 
AAGGCTGGCG AGCTCGTTAG CGAGAAAAAG TACCGTCAAC ATGTCAGCGA TCTGAAGTTC 
ACCAGCGTGG AGGACACTCC CGAGATGGTC CAGGCCAAAC TCAGCAACAA ACTGGCCCTA 
GACAGGCTGT ACAGGGAAAA GGGGGAACAT ATGAAGCACA ACTACACACT CTCCGGAGAT 
CTGCCTGAAC ACGTGCAGGC CAAGCTCAAT GCCATGAACA TCAGTGAGAC CCGCTATAAG 
GAGTCGTGGA CTAAAATCAG AGATGGTGGC TACAAGCTCC GTTTGGATGC CATTCCCTTC 
CAGTCAGCCA AAGCGTCTGG GAACATACTT AGTGATCACA AGTACAAGGA GCAGTTTGAA 
AAGACCAAGG GGCATATGAT TGGACTGAAG GGACTGGAGA ACGACATGAA CATGTCTCAC 
TCTGTGAATG CCAGTAAACT GCAGAGTGAC ATCAAGTATA AGCAGGACTC TGTGAATAAG 
CGCTCTCAGT ACCACCTTTC TATGGACATG TTGGAGATGG CCCATGCTAA AAAGGCCCAG 
ACCCTGGCCA GTGAGCAGGA CTACCGGCTT ACACTCCACC AGTACACTAC TCAACCCGAT 
GACATGAAGG TGCAGGCCGC TAAGAGAGCC TACCAACTAC AGAGCGAGAA AGTGTACCGC 
TCTGACCTGA ACTACCTGCG TGGTGTAGCC TGGATCAGCA CCGGGGCCCT CCAGATGGAG 
GGCTTCAAAA GGGCCACAGA CCTTATTAGT GAGAAAAACT ACCGTCAGCA GCCCTGCTCC 
TTCAAGCACA CATCTGTCGC AGACTCACCA GACATTGTCC ATGCCAAACT CAGCAATAAG 
CTTACCAACG AGCGCCTGTA CAAGATGAAA GGGGAGAATG ACAGGCATAA CTACACAATG 
ACATTGGAGA GACCAGAGAT AACACAGGCT AAGATCAACG CGGCTAACTT CAGCGAGACC 
AAGTACAGGG AGTCATGGCA CACTCTAAGG GCTCAGGGCT ACAAACTGAC CATGCAGGAC 
ATCCCCTTCC AGGCTGCCAA AACCTCAATG GATTTAGCCA GCGACGTCCA ATACAAGCAC 
CACCACCTGC TGGATAAGGG CAAGCACATC GGCGCCCGCA GCATTCTGGA TGACCCGCGC 
ATGCTACACT GCATGCAGAT GGGCCGGCTG ACGAGTGAGC AGGAGTACCG CAAAGACGCA 
CTTTCCACCA GCGGCCAGTA CCACCTCAAT CCAGATATGA TCAATTTAGT GACAGCCAAG 
AACGCCCAAG CTCTGGCCAG CGATCAGGAC TACAGGAAGA GGCTCCATGC GTACACTGTC 
CTCCCTGACG ATATTAAAGT CCAATGGGCC AAGAAGGCCT ATAACCTGCA GAGCGAGAAA 
CTGTACAAGT CGGATCTGAA CTTCATGAAG GGCGTGGCCT GGGATGGTGT GGGTGCGCCT 
CAAATGGAGT TGGCAAAGAA AGCCGGAGAA CTCACCAGTA ATAAGAAATA TCGTCAACTG 
CCAGACAGTC TGAAGTTCAC CCAAGTGGCG GACTCTCCAG ACATTGTTCA TGCTAAGAAC 
AGCTACCTCC AGTGCAGTGA GAGGCTGTAT AAGTCTGGAG ACTCGGACGC CATGCACAAA 
TACACCTTAC ATGCCGACGA CCCAGACTTC ATCAGAGCAA GGATCAACGC CCAGCAGATT 
AGCAATAAAG CCTATAAAGC GTCTGGGGAT GAGATGAGGT CGGCAGGCTA CGACTTGAGG 
CTGGATGCTA TTCCCTTCCA GACGGCCAAG GCTTCCACGA ATATCGCCAG TGATTTCCGC 
TACAAGGAAG CCTTTGTGAA GGAGAAGGGT CAGCAGGTGG GGCTGCTCAG TGTCGAAGAT 
GACCCAAAGA TGGTGCACTC CCTGGCAGCC AGCAAGCTCC AGAGCTGCCT GGGGTACAAG 
AGGCAGGCTC AAGAGACGCG CTCCCAGTTC AAGATGCATG CCAACCAGCC TGTGTTCCAG 
CAGGCCAAGA AGAGCCAAGC TCTGGCCAGC AACCAGACCT ATCACCAGAG GCTCCATGAC 
TACACCTTTG ACCCCCAGCA GCTCAACGTC CAACACGCCA AGCAGGCCTA CAAGCTGCAG 
AGTGACGTGA ACTACAAGTC AGATCTGAAC TGGATCAGGG GTGTGGGCTG GACCCCCCCT 
GGCTCCCATA AGGTGGAGAT GGCCCGGCGG GCTGCAGAAC TGGGCCTGAC GGAGAGCGTG 
AGCACTGAGG AGGCCATTGC CCAGTACCAA CACATGATGA TGATGCAGCA GATGGAGGAG 
CAGCAGCAGC TGTCAGAACC TGTTGAGACC AGCAAAGAGT TCCAGCAGTC GGGAGTCAAT 
CTAGACGCAA TGGAGATCCT CCACGTCAAG AGGAAGAAGA CCATCCAAAC GGTGAAGAAG 
TCCAATTCCT CCTCCATGGA TAGCAGCGTC AAGAAGTTAA TGATGTCTTC CAACTCAGCC 
ATAGAGTCAA TGATGGCTTC CACCTCTGTC TCAGCCATTG AGAACACAGT CAAGATGTCC 
ACAACGAGTG AGGCATCGGC CATAGAAAAT GCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrap ( XM_021575461.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021431136.1 Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X5, mRNA.

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CloneID	OOf25632
Clone ID Related Accession (Same CDS sequence)	XM_021575461.1
Accession Version	XM_021575461.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	nebulin-related-anchoring protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT GCGCCAGGTG TGGGTTTGTG GTGTACCCAG CGGAGAAGAT CAATTGTATT 
GATCAGAACT GGCACAAAGC ATGCTTTCAC TGCGACGTCT GTAAAATGGT GCTCACAGCC 
AAGAACTTTG TTAGCCACAA GAAGCGGCCA TACTGCTCTG TACACAATCC AAGAAACAAT 
ATGTTCACCA GTGTCTATGA GACCCCCATC AATGCCAAGA AGCAAGCCAT TGCCAGCAGT 
GAGCAGGACT ATCGGGCTAA GTTTGAGGAG CAGCAGACCT ACTCGTACTC AGGCACCATC 
ACCAACCAGG AGATTGTGAG CGTGACACAG GCCCAAAAGA CCATCAGTGA TGTCAAATAC 
ACTGAGGAAT ATGAGCAGAG TAAAGGAAAA GGCAGCTTAT CCACCATGAT CACACCAGGA 
TACCAGATTT CCAAGAAACC AAATACCTTG CCCAGCAGCG CGGAGTACAA TAAAGGAGAG 
GAGAAAGTGT CAAAATATTC CTCTGTAGCC AAAACCCCTG AAGTGCTTCT GGCCAAAAGC 
CAAGGACAGA TCCATTATGT CTACACTGAA GAGTATGAAC AGCAGTTGGG TAAAGGCAGT 
TTCCCCGCAC ATCTCACTCC TGGCTACCAA GTTTCAAAGA AAGCCACAGA GATGGCCAGT 
GGTGTGAAGT ACCGTCAGAT GTATGAGCAG GAGATGAAGG GAAAGGCCAC CTCAGATGCT 
GGGGCAGCAG AATTCGCTTC TGCCAGAGAA AATGCTGGGA ACTTCAGCCA GATTGCCTAC 
ACTGAAGAGT ACGAGCAGCA GAGAGGGAAG GGCAGTTTCC CAGCCATGAT CACTCCTGCC 
TACCAGCTTG CCAAGAAAGC CCAGGCCCAC GCCAGTGATC TGAAATACAA GAAGGACCTG 
AATAAGATGA AGGGCTCATC CCACTTCCAC AACCTGACCA GTGAGGACAA CCTGGCCCTG 
AAGAGCGCCC GCAAAATCAA CAAGATCGTC AGCGAGGTTG AGTACAAGAA GGACCTTGAG 
AACACTAAAG GCCACAGCAT CAACTACTGT GAGACGCCCC AGTACAAGAA TGCATCCAAG 
GCTGCCAAGT TCAGCAGTGA TATTGCCTAC AAGCAGGAGT ATGAGCAGGA GAAAACTGAC 
CATGTAGAAT ACAACTACCC AGCCACCATC ACACCAGGCT ACCAGAGTCA GAGGAAACTG 
GATCCACTCA AGGACAAGAA CTACAGACAG CACATTGACC AAGTCAAGTA CAGCTCGGTG 
ACAGATACCC CTGACATCGT CCAAGCCAGG ATCAACGCTC AACAACTCAG CAATGTTAAC 
TACAGACAAC ACATTGACCA AGTCAAGTAC AGCTCGGTGA CAGATACCCC TCAAATCGTC 
CAGGCCAGGA TCAACGCTCA ACAACTCAGC AATATAAAAT ACAAGGAGGA CTGGGAGAAG 
ACCAAGTCCA AAACATGTGA CATCGGCCTG GACACGCTGA CCGTCAAGGC TGCCAAAGCA 
TCTCGGGACC TGGCCAGTGA TATTAAGTAC AAAGAGTCAT TCATGAAAAA TAAAGATAAG 
ACAAGCAATG TCAGCGACTC CAAGACCCTG CACTCTCTTC AGGCGTCCAA GATGACCAGT 
GAGATTGAGT ACAAGAAGAA CTCCAAGGAG AACCAGTCCC AGTTCAGCTT GTCCATGGAC 
ATGGTCAACC TGAGCCATGC TAAAAAAGCC CAGGCCCTTG CCAGCGACCT GGACTACAGG 
ACGAAGCTCC ACCACTACAC CATCCTATCC GACGACATCC AAGTACAGCA GGCCAAAAAG 
GCCTATAACC TGCAGAGCGA GAATCAATAC CGTTCAGATC TGACCTGGAT GAAGGGAGTT 
GGCTGGGAGG CTGATGGATC TCTGAATGTG ACCCAAGCCA AGAAGGCTGG AGAGCTTCTC 
AGTGACAAAA AGTACCGTCA GAAAGTGGAC GACGTCAAGT TCACCCAGGT GGCCGATACT 
CCCTCTATCA AACACGCCAA GAAGAGCCAG GAACTGCAGA GCAAGCTAAC GTATAAGGCG 
GGCAACGAAC AGATGATGCA CCAGTACACC ATGGATAAAG ATGAGCCACT CTTCAGGCAG 
GCCAAAGCCA ATGCAGACCT CCTCAGTGGG AAAGTGTATA AGAGTAGCTG GGAGCAACAG 
CGAGAAAAGG GCTTTGAGTT GCGCATGGAC TCCTTGTCCA TCCTCACAGC TAAGGCTAAG 
AGAGACCTGG CCAGTAACGT GAAATACAAG GAGAAGTACG AGCAGACTAA GGGGAAGATG 
ATTGGGGTGA AGATGGTCAC TGACGACTCT CAGATGGCGC ACTCGGCTCT GGCCACCCGG 
TTGCAGAGTG ATCGCCACTA CAGAAAGAAG TACGAGGACA CTAAAACCAA GCATGGTGCC 
TCTTTGGACA TGTTGACCAT GACCCACGCC AAGAAGGCCC AGGATCTGGC AACTGAGACC 
AACTACAGGA CCTTCCTGCA CAAGTACACA ACCCTGCCCG GCGACATGAA GGTGGCCTGG 
GCCAAGAAGG CCTACGGACT ACTGAGTGAT AAACAGTACA GGTCGGATCG GAACTGGATG 
AAGGGCGTGG GCTGGGTGGC AGCAGATTTC CTGGATGTGA AACAAGCAAA GAAGGCTGGC 
GAGCTCGTTA GCGAGAAAAA GTACCGTCAA CATGTCAGCG ATCTGAAGTT CACCAGCGTG 
GAGGACACTC CCGAGATGGT CCAGGCCAAA CTCAGCAACA AACTGGCCCT AGACAGGCTG 
TACAGGGAAA AGGGGGAACA TATGAAGCAC AACTACACAC TCTCCGGAGA TCTGCCTGAA 
CACGTGCAGG CCAAGCTCAA TGCCATGAAC ATCAGTGAGA CCCGCTATAA GGAGTCGTGG 
ACTAAAATCA GAGATGGTGG CTACAAGCTC CGTTTGGATG CCATTCCCTT CCAGTCAGCC 
AAAGCGTCTG GGAACATACT TAGTGATCAC AAGTACAAGG AGCAGTTTGA AAAGACCAAG 
GGGCATATGA TTGGACTGAA GGGACTGGAG AACGACATGA ACATGTCTCA CTCTGTGAAT 
GCCAGTAAAC TGCAGAGTGA CATCAAGTAT AAGCAGGACT CTGTGAATAA GCGCTCTCAG 
TACCACCTTT CTATGGACAT GTTGGAGATG GCCCATGCTA AAAAGGCCCA GACCCTGGCC 
AGTGAGCAGG ACTACCGGCT TACACTCCAC CAGTACACTA CTCAACCCGA TGACATGAAG 
GTGCAGGCCG CTAAGAGAGC CTACCAACTA CAGAGCGAGA AAGTGTACCG CTCTGACCTG 
AACTACCTGC GTGGTGTAGC CTGGATCAGC ACCGGGGCCC TCCAGATGGA GGGCTTCAAA 
AGGGCCACAG ACCTTATTAG TGAGAAAAAC TACCGTCAGC AGCCCTGCTC CTTCAAGCAC 
ACATCTGTCG CAGACTCACC AGACATTGTC CATGCCAAAC TCAGCAATAA GCTTACCAAC 
GAGCGCCTGT ACAAGATGAA AGGGGAGAAT GACAGGCATA ACTACACAAT GACATTGGAG 
AGACCAGAGA TAACACAGGC TAAGATCAAC GCGGCTAACT TCAGCGAGAC CAAGTACAGG 
GAGTCATGGC ACACTCTAAG GGCTCAGGGC TACAAACTGA CCATGCAGGA CATCCCCTTC 
CAGGCTGCCA AAACCTCAAT GGATTTAGCC AGCGACGTCC AATACAAGCA CCACCACCTG 
CTGGATAAGG GCAAGCACAT CGGCGCCCGC AGCATTCTGG ATGACCCGCG CATGCTACAC 
TGCATGCAGA TGGGCCGGCT GACGAGTGAG CAGGAGTACC GCAAAGACGC ACTTTCCACC 
AGCGGCCAGT ACCACCTCAA TCCAGATATG ATCAATTTAG TGACAGCCAA GAACGCCCAA 
GCTCTGGCCA GCGATCAGGA CTACAGGAAG AGGCTCCATG CGTACACTGT CCTCCCTGAC 
GATATTAAAG TCCAATGGGC CAAGAAGGCC TATAACCTGC AGAGCGAGAA ACTGTACAAG 
TCGGATCTGA ACTTCATGAA GGGCGTGGCC TGGGATGGTG TGGGTGCGCC TCAAATGGAG 
TTGGCAAAGA AAGCCGGAGA ACTCACCAGT AATAAGAAAT ATCGTCAACT GCCAGACAGT 
CTGAAGTTCA CCCAAGTGGC GGACTCTCCA GACATTGTTC ATGCTAAGAA CAGCTACCTC 
CAGTGCAGTG AGAGGCTGTA TAAGTCTGGA GACTCGGACG CCATGCACAA ATACACCTTA 
CATGCCGACG ACCCAGACTT CATCAGAGCA AGGATCAACG CCCAGCAGAT TAGCAATAAA 
GCCTATAAAG CGTCTGGGGA TGAGATGAGG TCGGCAGGCT ACGACTTGAG GCTGGATGCT 
ATTCCCTTCC AGACGGCCAA GGCTTCCACG AATATCGCCA GTGATTTCCG CTACAAGGAA 
GCCTTTGTGA AGGAGAAGGG TCAGCAGGTG GGGCTGCTCA GTGTCGAAGA TGACCCAAAG 
ATGGTGCACT CCCTGGCAGC CAGCAAGCTC CAGAGCTGCC TGGGGTACAA GAGGCAGGCT 
CAAGAGACGC GCTCCCAGTT CAAGATGCAT GCCAACCAGC CTGTGTTCCA GCAGGCCAAG 
AAGAGCCAAG CTCTGGCCAG CAACCAGACC TATCACCAGA GGCTCCATGA CTACACCTTT 
GACCCCCAGC AGCTCAACGT CCAACACGCC AAGCAGGCCT ACAAGCTGCA GAGTGACGTG 
AACTACAAGT CAGATCTGAA CTGGATCAGG GGTGTGGGCT GGACCCCCCC TGGCTCCCAT 
AAGGTGGAGA TGGCCCGGCG GGCTGCAGAA CTGGGCCTGA CGGAGAGCGT GAGCACTGAG 
GAGGCCATTG CCCAGTACCA ACACATGATG ATGATGCAGC AGATGGAGGA GCAGCAGCAG 
CTGTCAGAAC CTGTTGAGAC CAGCAAAGAG TTCCAGCAGT CGGGAGTCAA TCTAGACGCA 
ATGGAGATCC TCCACGTCAA GAGGAAGAAG ACCATCCAAA CGGTGAAGAA GTCCAATTCC 
TCCTCCATGG ATAGCAGCGT CAAGAAGTTA ATGATGTCTT CCAACTCAGC CATAGAGTCA 
ATGATGGCTT CCACCTCTGT CTCAGCCATT GAGAACACAG TCAAGATGTC CACAACGAGT 
GAGGCATCGG CCATAGAAAA TGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021431136.1
CDS	198..5384
Translation	MQSCARCGFVVYPAEKINCIDQNWHKACFHCDVCKMVLTAKNFVSHKKRPYCSVHNPRNNMFTSVYETPINAKKQAIASSEQDYRAKFEEQQTYSYSGTITNQEIVSVTQAQKTISDVKYTEEYEQSKGKGSLSTMITPGYQISKKPNTLPSSAEYNKGEEKVSKYSSVAKTPEVLLAKSQGQIHYVYTEEYEQQLGKGSFPAHLTPGYQVSKKATEMASGVKYRQMYEQEMKGKATSDAGAAEFASARENAGNFSQIAYTEEYEQQRGKGSFPAMITPAYQLAKKAQAHASDLKYKKDLNKMKGSSHFHNLTSEDNLALKSARKINKIVSEVEYKKDLENTKGHSINYCETPQYKNASKAAKFSSDIAYKQEYEQEKTDHVEYNYPATITPGYQSQRKLDPLKDKNYRQHIDQVKYSSVTDTPDIVQARINAQQLSNVNYRQHIDQVKYSSVTDTPQIVQARINAQQLSNIKYKEDWEKTKSKTCDIGLDTLTVKAAKASRDLASDIKYKESFMKNKDKTSNVSDSKTLHSLQASKMTSEIEYKKNSKENQSQFSLSMDMVNLSHAKKAQALASDLDYRTKLHHYTILSDDIQVQQAKKAYNLQSENQYRSDLTWMKGVGWEADGSLNVTQAKKAGELLSDKKYRQKVDDVKFTQVADTPSIKHAKKSQELQSKLTYKAGNEQMMHQYTMDKDEPLFRQAKANADLLSGKVYKSSWEQQREKGFELRMDSLSILTAKAKRDLASNVKYKEKYEQTKGKMIGVKMVTDDSQMAHSALATRLQSDRHYRKKYEDTKTKHGASLDMLTMTHAKKAQDLATETNYRTFLHKYTTLPGDMKVAWAKKAYGLLSDKQYRSDRNWMKGVGWVAADFLDVKQAKKAGELVSEKKYRQHVSDLKFTSVEDTPEMVQAKLSNKLALDRLYREKGEHMKHNYTLSGDLPEHVQAKLNAMNISETRYKESWTKIRDGGYKLRLDAIPFQSAKASGNILSDHKYKEQFEKTKGHMIGLKGLENDMNMSHSVNASKLQSDIKYKQDSVNKRSQYHLSMDMLEMAHAKKAQTLASEQDYRLTLHQYTTQPDDMKVQAAKRAYQLQSEKVYRSDLNYLRGVAWISTGALQMEGFKRATDLISEKNYRQQPCSFKHTSVADSPDIVHAKLSNKLTNERLYKMKGENDRHNYTMTLERPEITQAKINAANFSETKYRESWHTLRAQGYKLTMQDIPFQAAKTSMDLASDVQYKHHHLLDKGKHIGARSILDDPRMLHCMQMGRLTSEQEYRKDALSTSGQYHLNPDMINLVTAKNAQALASDQDYRKRLHAYTVLPDDIKVQWAKKAYNLQSEKLYKSDLNFMKGVAWDGVGAPQMELAKKAGELTSNKKYRQLPDSLKFTQVADSPDIVHAKNSYLQCSERLYKSGDSDAMHKYTLHADDPDFIRARINAQQISNKAYKASGDEMRSAGYDLRLDAIPFQTAKASTNIASDFRYKEAFVKEKGQQVGLLSVEDDPKMVHSLAASKLQSCLGYKRQAQETRSQFKMHANQPVFQQAKKSQALASNQTYHQRLHDYTFDPQQLNVQHAKQAYKLQSDVNYKSDLNWIRGVGWTPPGSHKVEMARRAAELGLTESVSTEEAIAQYQHMMMMQQMEEQQQLSEPVETSKEFQQSGVNLDAMEILHVKRKKTIQTVKKSNSSSMDSSVKKLMMSSNSAIESMMASTSVSAIENTVKMSTTSEASAIENA

Target ORF information:

RefSeq Version	XM_021575461.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021575461.1

ORF Insert Sequence:

ATGCAGTCCT GCGCCAGGTG TGGGTTTGTG GTGTACCCAG CGGAGAAGAT CAATTGTATT 
GATCAGAACT GGCACAAAGC ATGCTTTCAC TGCGACGTCT GTAAAATGGT GCTCACAGCC 
AAGAACTTTG TTAGCCACAA GAAGCGGCCA TACTGCTCTG TACACAATCC AAGAAACAAT 
ATGTTCACCA GTGTCTATGA GACCCCCATC AATGCCAAGA AGCAAGCCAT TGCCAGCAGT 
GAGCAGGACT ATCGGGCTAA GTTTGAGGAG CAGCAGACCT ACTCGTACTC AGGCACCATC 
ACCAACCAGG AGATTGTGAG CGTGACACAG GCCCAAAAGA CCATCAGTGA TGTCAAATAC 
ACTGAGGAAT ATGAGCAGAG TAAAGGAAAA GGCAGCTTAT CCACCATGAT CACACCAGGA 
TACCAGATTT CCAAGAAACC AAATACCTTG CCCAGCAGCG CGGAGTACAA TAAAGGAGAG 
GAGAAAGTGT CAAAATATTC CTCTGTAGCC AAAACCCCTG AAGTGCTTCT GGCCAAAAGC 
CAAGGACAGA TCCATTATGT CTACACTGAA GAGTATGAAC AGCAGTTGGG TAAAGGCAGT 
TTCCCCGCAC ATCTCACTCC TGGCTACCAA GTTTCAAAGA AAGCCACAGA GATGGCCAGT 
GGTGTGAAGT ACCGTCAGAT GTATGAGCAG GAGATGAAGG GAAAGGCCAC CTCAGATGCT 
GGGGCAGCAG AATTCGCTTC TGCCAGAGAA AATGCTGGGA ACTTCAGCCA GATTGCCTAC 
ACTGAAGAGT ACGAGCAGCA GAGAGGGAAG GGCAGTTTCC CAGCCATGAT CACTCCTGCC 
TACCAGCTTG CCAAGAAAGC CCAGGCCCAC GCCAGTGATC TGAAATACAA GAAGGACCTG 
AATAAGATGA AGGGCTCATC CCACTTCCAC AACCTGACCA GTGAGGACAA CCTGGCCCTG 
AAGAGCGCCC GCAAAATCAA CAAGATCGTC AGCGAGGTTG AGTACAAGAA GGACCTTGAG 
AACACTAAAG GCCACAGCAT CAACTACTGT GAGACGCCCC AGTACAAGAA TGCATCCAAG 
GCTGCCAAGT TCAGCAGTGA TATTGCCTAC AAGCAGGAGT ATGAGCAGGA GAAAACTGAC 
CATGTAGAAT ACAACTACCC AGCCACCATC ACACCAGGCT ACCAGAGTCA GAGGAAACTG 
GATCCACTCA AGGACAAGAA CTACAGACAG CACATTGACC AAGTCAAGTA CAGCTCGGTG 
ACAGATACCC CTGACATCGT CCAAGCCAGG ATCAACGCTC AACAACTCAG CAATGTTAAC 
TACAGACAAC ACATTGACCA AGTCAAGTAC AGCTCGGTGA CAGATACCCC TCAAATCGTC 
CAGGCCAGGA TCAACGCTCA ACAACTCAGC AATATAAAAT ACAAGGAGGA CTGGGAGAAG 
ACCAAGTCCA AAACATGTGA CATCGGCCTG GACACGCTGA CCGTCAAGGC TGCCAAAGCA 
TCTCGGGACC TGGCCAGTGA TATTAAGTAC AAAGAGTCAT TCATGAAAAA TAAAGATAAG 
ACAAGCAATG TCAGCGACTC CAAGACCCTG CACTCTCTTC AGGCGTCCAA GATGACCAGT 
GAGATTGAGT ACAAGAAGAA CTCCAAGGAG AACCAGTCCC AGTTCAGCTT GTCCATGGAC 
ATGGTCAACC TGAGCCATGC TAAAAAAGCC CAGGCCCTTG CCAGCGACCT GGACTACAGG 
ACGAAGCTCC ACCACTACAC CATCCTATCC GACGACATCC AAGTACAGCA GGCCAAAAAG 
GCCTATAACC TGCAGAGCGA GAATCAATAC CGTTCAGATC TGACCTGGAT GAAGGGAGTT 
GGCTGGGAGG CTGATGGATC TCTGAATGTG ACCCAAGCCA AGAAGGCTGG AGAGCTTCTC 
AGTGACAAAA AGTACCGTCA GAAAGTGGAC GACGTCAAGT TCACCCAGGT GGCCGATACT 
CCCTCTATCA AACACGCCAA GAAGAGCCAG GAACTGCAGA GCAAGCTAAC GTATAAGGCG 
GGCAACGAAC AGATGATGCA CCAGTACACC ATGGATAAAG ATGAGCCACT CTTCAGGCAG 
GCCAAAGCCA ATGCAGACCT CCTCAGTGGG AAAGTGTATA AGAGTAGCTG GGAGCAACAG 
CGAGAAAAGG GCTTTGAGTT GCGCATGGAC TCCTTGTCCA TCCTCACAGC TAAGGCTAAG 
AGAGACCTGG CCAGTAACGT GAAATACAAG GAGAAGTACG AGCAGACTAA GGGGAAGATG 
ATTGGGGTGA AGATGGTCAC TGACGACTCT CAGATGGCGC ACTCGGCTCT GGCCACCCGG 
TTGCAGAGTG ATCGCCACTA CAGAAAGAAG TACGAGGACA CTAAAACCAA GCATGGTGCC 
TCTTTGGACA TGTTGACCAT GACCCACGCC AAGAAGGCCC AGGATCTGGC AACTGAGACC 
AACTACAGGA CCTTCCTGCA CAAGTACACA ACCCTGCCCG GCGACATGAA GGTGGCCTGG 
GCCAAGAAGG CCTACGGACT ACTGAGTGAT AAACAGTACA GGTCGGATCG GAACTGGATG 
AAGGGCGTGG GCTGGGTGGC AGCAGATTTC CTGGATGTGA AACAAGCAAA GAAGGCTGGC 
GAGCTCGTTA GCGAGAAAAA GTACCGTCAA CATGTCAGCG ATCTGAAGTT CACCAGCGTG 
GAGGACACTC CCGAGATGGT CCAGGCCAAA CTCAGCAACA AACTGGCCCT AGACAGGCTG 
TACAGGGAAA AGGGGGAACA TATGAAGCAC AACTACACAC TCTCCGGAGA TCTGCCTGAA 
CACGTGCAGG CCAAGCTCAA TGCCATGAAC ATCAGTGAGA CCCGCTATAA GGAGTCGTGG 
ACTAAAATCA GAGATGGTGG CTACAAGCTC CGTTTGGATG CCATTCCCTT CCAGTCAGCC 
AAAGCGTCTG GGAACATACT TAGTGATCAC AAGTACAAGG AGCAGTTTGA AAAGACCAAG 
GGGCATATGA TTGGACTGAA GGGACTGGAG AACGACATGA ACATGTCTCA CTCTGTGAAT 
GCCAGTAAAC TGCAGAGTGA CATCAAGTAT AAGCAGGACT CTGTGAATAA GCGCTCTCAG 
TACCACCTTT CTATGGACAT GTTGGAGATG GCCCATGCTA AAAAGGCCCA GACCCTGGCC 
AGTGAGCAGG ACTACCGGCT TACACTCCAC CAGTACACTA CTCAACCCGA TGACATGAAG 
GTGCAGGCCG CTAAGAGAGC CTACCAACTA CAGAGCGAGA AAGTGTACCG CTCTGACCTG 
AACTACCTGC GTGGTGTAGC CTGGATCAGC ACCGGGGCCC TCCAGATGGA GGGCTTCAAA 
AGGGCCACAG ACCTTATTAG TGAGAAAAAC TACCGTCAGC AGCCCTGCTC CTTCAAGCAC 
ACATCTGTCG CAGACTCACC AGACATTGTC CATGCCAAAC TCAGCAATAA GCTTACCAAC 
GAGCGCCTGT ACAAGATGAA AGGGGAGAAT GACAGGCATA ACTACACAAT GACATTGGAG 
AGACCAGAGA TAACACAGGC TAAGATCAAC GCGGCTAACT TCAGCGAGAC CAAGTACAGG 
GAGTCATGGC ACACTCTAAG GGCTCAGGGC TACAAACTGA CCATGCAGGA CATCCCCTTC 
CAGGCTGCCA AAACCTCAAT GGATTTAGCC AGCGACGTCC AATACAAGCA CCACCACCTG 
CTGGATAAGG GCAAGCACAT CGGCGCCCGC AGCATTCTGG ATGACCCGCG CATGCTACAC 
TGCATGCAGA TGGGCCGGCT GACGAGTGAG CAGGAGTACC GCAAAGACGC ACTTTCCACC 
AGCGGCCAGT ACCACCTCAA TCCAGATATG ATCAATTTAG TGACAGCCAA GAACGCCCAA 
GCTCTGGCCA GCGATCAGGA CTACAGGAAG AGGCTCCATG CGTACACTGT CCTCCCTGAC 
GATATTAAAG TCCAATGGGC CAAGAAGGCC TATAACCTGC AGAGCGAGAA ACTGTACAAG 
TCGGATCTGA ACTTCATGAA GGGCGTGGCC TGGGATGGTG TGGGTGCGCC TCAAATGGAG 
TTGGCAAAGA AAGCCGGAGA ACTCACCAGT AATAAGAAAT ATCGTCAACT GCCAGACAGT 
CTGAAGTTCA CCCAAGTGGC GGACTCTCCA GACATTGTTC ATGCTAAGAA CAGCTACCTC 
CAGTGCAGTG AGAGGCTGTA TAAGTCTGGA GACTCGGACG CCATGCACAA ATACACCTTA 
CATGCCGACG ACCCAGACTT CATCAGAGCA AGGATCAACG CCCAGCAGAT TAGCAATAAA 
GCCTATAAAG CGTCTGGGGA TGAGATGAGG TCGGCAGGCT ACGACTTGAG GCTGGATGCT 
ATTCCCTTCC AGACGGCCAA GGCTTCCACG AATATCGCCA GTGATTTCCG CTACAAGGAA 
GCCTTTGTGA AGGAGAAGGG TCAGCAGGTG GGGCTGCTCA GTGTCGAAGA TGACCCAAAG 
ATGGTGCACT CCCTGGCAGC CAGCAAGCTC CAGAGCTGCC TGGGGTACAA GAGGCAGGCT 
CAAGAGACGC GCTCCCAGTT CAAGATGCAT GCCAACCAGC CTGTGTTCCA GCAGGCCAAG 
AAGAGCCAAG CTCTGGCCAG CAACCAGACC TATCACCAGA GGCTCCATGA CTACACCTTT 
GACCCCCAGC AGCTCAACGT CCAACACGCC AAGCAGGCCT ACAAGCTGCA GAGTGACGTG 
AACTACAAGT CAGATCTGAA CTGGATCAGG GGTGTGGGCT GGACCCCCCC TGGCTCCCAT 
AAGGTGGAGA TGGCCCGGCG GGCTGCAGAA CTGGGCCTGA CGGAGAGCGT GAGCACTGAG 
GAGGCCATTG CCCAGTACCA ACACATGATG ATGATGCAGC AGATGGAGGA GCAGCAGCAG 
CTGTCAGAAC CTGTTGAGAC CAGCAAAGAG TTCCAGCAGT CGGGAGTCAA TCTAGACGCA 
ATGGAGATCC TCCACGTCAA GAGGAAGAAG ACCATCCAAA CGGTGAAGAA GTCCAATTCC 
TCCTCCATGG ATAGCAGCGT CAAGAAGTTA ATGATGTCTT CCAACTCAGC CATAGAGTCA 
ATGATGGCTT CCACCTCTGT CTCAGCCATT GAGAACACAG TCAAGATGTC CACAACGAGT 
GAGGCATCGG CCATAGAAAA TGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrap ( XM_021575462.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021431137.1 Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X6, mRNA.

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CloneID	OOf25633
Clone ID Related Accession (Same CDS sequence)	XM_021575462.1
Accession Version	XM_021575462.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	nebulin-related-anchoring protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCCT GCGCCAGGTG TGGGTTTGTG GTGTACCCAG CGGAGAAGAT CAATTGTATT 
GATCAGAACT GGCACAAAGC ATGCTTTCAC TGCGACGTCT GTAAAATGGT GCTCACAGCC 
AAGAACTTTG TTAGCCACAA GAAGCGGCCA TACTGCTCTG TACACAATCC AAGAAACAAT 
ATGTTCACCA GTGTCTATGA GACCCCCATC AATGCCAAGA AGCAAGCCAT TGCCAGCAGT 
GAGCAGGACT ATCGGGCTAA GTTTGAGGAG CAGCAGACCT ACTCGTACTC AGGCACCATC 
ACCAACCAGG AGATTGTGAG CGTGACACAG GCCCAAAAGA CCATCAGTGA TTATGTCTAC 
ACTGAAGAGT ATGAACAGCA GTTGGGTAAA GGCAGTTTCC CCGCACATCT CACTCCTGGC 
TACCAAGTTT CAAAGAAAGC CACAGAGATG GCCAGTGGTG TGAAGTACCG TCAGATGTAT 
GAGCAGGAGA TGAAGGGAAA GGCCACCTCA GATGCTGGGG CAGCAGAATT CGCTTCTGCC 
AGAGAAAATG CTGGGAACTT CAGCCAGATT GCCTACACTG AAGAGTACGA GCAGCAGAGA 
GGGAAGGGCA GTTTCCCAGC CATGATCACT CCTGCCTACC AGCTTGCCAA GAAAGCCCAG 
GCCCACGCCA GTGATCTGAA ATACAAGAAG GACCTGAATA AGATGAAGGG CTCATCCCAC 
TTCCACAACC TGACCAGTGA GGACAACCTG GCCCTGAAGA GCGCCCGCAA AATCAACAAG 
ATCGTCAGCG AGGTTGAGTA CAAGAAGGAC CTTGAGAACA CTAAAGGCCA CAGCATCAAC 
TACTGTGAGA CGCCCCAGTA CAAGAATGCA TCCAAGGCTG CCAAGTTCAG CAGTGATAAC 
AAGTACAAGG AGAAGTATAC CAACCACATG AAAGGGCACT ATGAAGGCTC TGAGATGGAC 
AAGAATGCTG TACACGCTAT GAACGTCAGA AAGCTGGCAA GCAATATTGC CTACAAGCAG 
GAGTATGAGC AGGAGAAAAC TGACCATGTA GAATACAACT ACCCAGCCAC CATCACACCA 
GGCTACCAGA GTCAGAGGAA ACTGGATCCA CTCAAGGACA AGAACTACAG ACAGCACATT 
GACCAAGTCA AGTACAGCTC GGTGACAGAT ACCCCTGACA TCGTCCAAGC CAGGATCAAC 
GCTCAACAAC TCAGCAATGT TAACTACAGA CAACACATTG ACCAAGTCAA GTACAGCTCG 
GTGACAGATA CCCCTCAAAT CGTCCAGGCC AGGATCAACG CTCAACAACT CAGCAATATA 
AAATACAAGG AGGACTGGGA GAAGACCAAG TCCAAAACAT GTGACATCGG CCTGGACACG 
CTGACCGTCA AGGCTGCCAA AGCATCTCGG GACCTGGCCA GTGATATTAA GTACAAAGAG 
TCATTCATGA AAAATAAAGA TAAGACAAGC AATGTCAGCG ACTCCAAGAC CCTGCACTCT 
CTTCAGGCGT CCAAGATGAC CAGTGAGATT GAGTACAAGA AGAACTCCAA GGAGAACCAG 
TCCCAGTTCA GCTTGTCCAT GGACATGGTC AACCTGAGCC ATGCTAAAAA AGCCCAGGCC 
CTTGCCAGCG ACCTGGACTA CAGGACGAAG CTCCACCACT ACACCATCCT ATCCGACGAC 
ATCCAAGTAC AGCAGGCCAA AAAGGCCTAT AACCTGCAGA GCGAGAATCA ATACCGTTCA 
GATCTGACCT GGATGAAGGG AGTTGGCTGG GAGGCTGATG GATCTCTGAA TGTGACCCAA 
GCCAAGAAGG CTGGAGAGCT TCTCAGTGAC AAAAAGTACC GTCAGAAAGT GGACGACGTC 
AAGTTCACCC AGGTGGCCGA TACTCCCTCT ATCAAACACG CCAAGAAGAG CCAGGAACTG 
CAGAGCAAGC TAACGTATAA GGCGGGCAAC GAACAGATGA TGCACCAGTA CACCATGGAT 
AAAGATGAGC CACTCTTCAG GCAGGCCAAA GCCAATGCAG ACCTCCTCAG TGGGAAAGTG 
TATAAGAGTA GCTGGGAGCA ACAGCGAGAA AAGGGCTTTG AGTTGCGCAT GGACTCCTTG 
TCCATCCTCA CAGCTAAGGC TAAGAGAGAC CTGGCCAGTA ACGTGAAATA CAAGGAGAAG 
TACGAGCAGA CTAAGGGGAA GATGATTGGG GTGAAGATGG TCACTGACGA CTCTCAGATG 
GCGCACTCGG CTCTGGCCAC CCGGTTGCAG AGTGATCGCC ACTACAGAAA GAAGTACGAG 
GACACTAAAA CCAAGCATGG TGCCTCTTTG GACATGTTGA CCATGACCCA CGCCAAGAAG 
GCCCAGGATC TGGCAACTGA GACCAACTAC AGGACCTTCC TGCACAAGTA CACAACCCTG 
CCCGGCGACA TGAAGGTGGC CTGGGCCAAG AAGGCCTACG GACTACTGAG TGATAAACAG 
TACAGGTCGG ATCGGAACTG GATGAAGGGC GTGGGCTGGG TGGCAGCAGA TTTCCTGGAT 
GTGAAACAAG CAAAGAAGGC TGGCGAGCTC GTTAGCGAGA AAAAGTACCG TCAACATGTC 
AGCGATCTGA AGTTCACCAG CGTGGAGGAC ACTCCCGAGA TGGTCCAGGC CAAACTCAGC 
AACAAACTGG CCCTAGACAG GCTGTACAGG GAAAAGGGGG AACATATGAA GCACAACTAC 
ACACTCTCCG GAGATCTGCC TGAACACGTG CAGGCCAAGC TCAATGCCAT GAACATCAGT 
GAGACCCGCT ATAAGGAGTC GTGGACTAAA ATCAGAGATG GTGGCTACAA GCTCCGTTTG 
GATGCCATTC CCTTCCAGTC AGCCAAAGCG TCTGGGAACA TACTTAGTGA TCACAAGTAC 
AAGGAGCAGT TTGAAAAGAC CAAGGGGCAT ATGATTGGAC TGAAGGGACT GGAGAACGAC 
ATGAACATGT CTCACTCTGT GAATGCCAGT AAACTGCAGA GTGACATCAA GTATAAGCAG 
GACTCTGTGA ATAAGCGCTC TCAGTACCAC CTTTCTATGG ACATGTTGGA GATGGCCCAT 
GCTAAAAAGG CCCAGACCCT GGCCAGTGAG CAGGACTACC GGCTTACACT CCACCAGTAC 
ACTACTCAAC CCGATGACAT GAAGGTGCAG GCCGCTAAGA GAGCCTACCA ACTACAGAGC 
GAGAAAGTGT ACCGCTCTGA CCTGAACTAC CTGCGTGGTG TAGCCTGGAT CAGCACCGGG 
GCCCTCCAGA TGGAGGGCTT CAAAAGGGCC ACAGACCTTA TTAGTGAGAA AAACTACCGT 
CAGCAGCCCT GCTCCTTCAA GCACACATCT GTCGCAGACT CACCAGACAT TGTCCATGCC 
AAACTCAGCA ATAAGCTTAC CAACGAGCGC CTGTACAAGA TGAAAGGGGA GAATGACAGG 
CATAACTACA CAATGACATT GGAGAGACCA GAGATAACAC AGGCTAAGAT CAACGCGGCT 
AACTTCAGCG AGACCAAGTA CAGGGAGTCA TGGCACACTC TAAGGGCTCA GGGCTACAAA 
CTGACCATGC AGGACATCCC CTTCCAGGCT GCCAAAACCT CAATGGATTT AGCCAGCGAC 
GTCCAATACA AGCACCACCA CCTGCTGGAT AAGGGCAAGC ACATCGGCGC CCGCAGCATT 
CTGGATGACC CGCGCATGCT ACACTGCATG CAGATGGGCC GGCTGACGAG TGAGCAGGAG 
TACCGCAAAG ACGCACTTTC CACCAGCGGC CAGTACCACC TCAATCCAGA TATGATCAAT 
TTAGTGACAG CCAAGAACGC CCAAGCTCTG GCCAGCGATC AGGACTACAG GAAGAGGCTC 
CATGCGTACA CTGTCCTCCC TGACGATATT AAAGTCCAAT GGGCCAAGAA GGCCTATAAC 
CTGCAGAGCG AGAAACTGTA CAAGTCGGAT CTGAACTTCA TGAAGGGCGT GGCCTGGGAT 
GGTGTGGGTG CGCCTCAAAT GGAGTTGGCA AAGAAAGCCG GAGAACTCAC CAGTAATAAG 
AAATATCGTC AACTGCCAGA CAGTCTGAAG TTCACCCAAG TGGCGGACTC TCCAGACATT 
GTTCATGCTA AGAACAGCTA CCTCCAGTGC AGTGAGAGGC TGTATAAGTC TGGAGACTCG 
GACGCCATGC ACAAATACAC CTTACATGCC GACGACCCAG ACTTCATCAG AGCAAGGATC 
AACGCCCAGC AGATTAGCAA TAAAGCCTAT AAAGCGTCTG GGGATGAGAT GAGGTCGGCA 
GGCTACGACT TGAGGCTGGA TGCTATTCCC TTCCAGACGG CCAAGGCTTC CACGAATATC 
GCCAGTGATT TCCGCTACAA GGAAGCCTTT GTGAAGGAGA AGGGTCAGCA GGTGGGGCTG 
CTCAGTGTCG AAGATGACCC AAAGATGGTG CACTCCCTGG CAGCCAGCAA GCTCCAGAGC 
TGCCTGGGGT ACAAGAGGCA GGCTCAAGAG ACGCGCTCCC AGTTCAAGAT GCATGCCAAC 
CAGCCTGTGT TCCAGCAGGC CAAGAAGAGC CAAGCTCTGG CCAGCAACCA GACCTATCAC 
CAGAGGCTCC ATGACTACAC CTTTGACCCC CAGCAGCTCA ACGTCCAACA CGCCAAGCAG 
GCCTACAAGC TGCAGAGTGA CGTGAACTAC AAGTCAGATC TGAACTGGAT CAGGGGTGTG 
GGCTGGACCC CCCCTGGCTC CCATAAGGTG GAGATGGCCC GGCGGGCTGC AGAACTGGGC 
CTGACGGAGA GCGTGAGCAC TGAGGAGGCC ATTGCCCAGT ACCAACACAT GATGATGATG 
CAGCAGATGG AGGAGCAGCA GCAGCTGTCA GAACCTGTTG AGACCAGCAA AGAGTTCCAG 
CAGTCGGGAG TCAATCTAGA CGCAATGGAG ATCCTCCACG TCAAGAGGAA GAAGACCATC 
CAAACGGTGA AGAAGTCCAA TTCCTCCTCC ATGGATAGCA GCGTCAAGAA GTTAATGATG 
TCTTCCAACT CAGCCATAGA GTCAATGATG GCTTCCACCT CTGTCTCAGC CATTGAGAAC 
ACAGTCAAGA TGTCCACAAC GAGTGAGGCA TCGGCCATAG AAAATGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021431137.1
CDS	198..5288
Translation	MQSCARCGFVVYPAEKINCIDQNWHKACFHCDVCKMVLTAKNFVSHKKRPYCSVHNPRNNMFTSVYETPINAKKQAIASSEQDYRAKFEEQQTYSYSGTITNQEIVSVTQAQKTISDYVYTEEYEQQLGKGSFPAHLTPGYQVSKKATEMASGVKYRQMYEQEMKGKATSDAGAAEFASARENAGNFSQIAYTEEYEQQRGKGSFPAMITPAYQLAKKAQAHASDLKYKKDLNKMKGSSHFHNLTSEDNLALKSARKINKIVSEVEYKKDLENTKGHSINYCETPQYKNASKAAKFSSDNKYKEKYTNHMKGHYEGSEMDKNAVHAMNVRKLASNIAYKQEYEQEKTDHVEYNYPATITPGYQSQRKLDPLKDKNYRQHIDQVKYSSVTDTPDIVQARINAQQLSNVNYRQHIDQVKYSSVTDTPQIVQARINAQQLSNIKYKEDWEKTKSKTCDIGLDTLTVKAAKASRDLASDIKYKESFMKNKDKTSNVSDSKTLHSLQASKMTSEIEYKKNSKENQSQFSLSMDMVNLSHAKKAQALASDLDYRTKLHHYTILSDDIQVQQAKKAYNLQSENQYRSDLTWMKGVGWEADGSLNVTQAKKAGELLSDKKYRQKVDDVKFTQVADTPSIKHAKKSQELQSKLTYKAGNEQMMHQYTMDKDEPLFRQAKANADLLSGKVYKSSWEQQREKGFELRMDSLSILTAKAKRDLASNVKYKEKYEQTKGKMIGVKMVTDDSQMAHSALATRLQSDRHYRKKYEDTKTKHGASLDMLTMTHAKKAQDLATETNYRTFLHKYTTLPGDMKVAWAKKAYGLLSDKQYRSDRNWMKGVGWVAADFLDVKQAKKAGELVSEKKYRQHVSDLKFTSVEDTPEMVQAKLSNKLALDRLYREKGEHMKHNYTLSGDLPEHVQAKLNAMNISETRYKESWTKIRDGGYKLRLDAIPFQSAKASGNILSDHKYKEQFEKTKGHMIGLKGLENDMNMSHSVNASKLQSDIKYKQDSVNKRSQYHLSMDMLEMAHAKKAQTLASEQDYRLTLHQYTTQPDDMKVQAAKRAYQLQSEKVYRSDLNYLRGVAWISTGALQMEGFKRATDLISEKNYRQQPCSFKHTSVADSPDIVHAKLSNKLTNERLYKMKGENDRHNYTMTLERPEITQAKINAANFSETKYRESWHTLRAQGYKLTMQDIPFQAAKTSMDLASDVQYKHHHLLDKGKHIGARSILDDPRMLHCMQMGRLTSEQEYRKDALSTSGQYHLNPDMINLVTAKNAQALASDQDYRKRLHAYTVLPDDIKVQWAKKAYNLQSEKLYKSDLNFMKGVAWDGVGAPQMELAKKAGELTSNKKYRQLPDSLKFTQVADSPDIVHAKNSYLQCSERLYKSGDSDAMHKYTLHADDPDFIRARINAQQISNKAYKASGDEMRSAGYDLRLDAIPFQTAKASTNIASDFRYKEAFVKEKGQQVGLLSVEDDPKMVHSLAASKLQSCLGYKRQAQETRSQFKMHANQPVFQQAKKSQALASNQTYHQRLHDYTFDPQQLNVQHAKQAYKLQSDVNYKSDLNWIRGVGWTPPGSHKVEMARRAAELGLTESVSTEEAIAQYQHMMMMQQMEEQQQLSEPVETSKEFQQSGVNLDAMEILHVKRKKTIQTVKKSNSSSMDSSVKKLMMSSNSAIESMMASTSVSAIENTVKMSTTSEASAIENA

Target ORF information:

RefSeq Version	XM_021575462.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021575462.1

ORF Insert Sequence:

ATGCAGTCCT GCGCCAGGTG TGGGTTTGTG GTGTACCCAG CGGAGAAGAT CAATTGTATT 
GATCAGAACT GGCACAAAGC ATGCTTTCAC TGCGACGTCT GTAAAATGGT GCTCACAGCC 
AAGAACTTTG TTAGCCACAA GAAGCGGCCA TACTGCTCTG TACACAATCC AAGAAACAAT 
ATGTTCACCA GTGTCTATGA GACCCCCATC AATGCCAAGA AGCAAGCCAT TGCCAGCAGT 
GAGCAGGACT ATCGGGCTAA GTTTGAGGAG CAGCAGACCT ACTCGTACTC AGGCACCATC 
ACCAACCAGG AGATTGTGAG CGTGACACAG GCCCAAAAGA CCATCAGTGA TTATGTCTAC 
ACTGAAGAGT ATGAACAGCA GTTGGGTAAA GGCAGTTTCC CCGCACATCT CACTCCTGGC 
TACCAAGTTT CAAAGAAAGC CACAGAGATG GCCAGTGGTG TGAAGTACCG TCAGATGTAT 
GAGCAGGAGA TGAAGGGAAA GGCCACCTCA GATGCTGGGG CAGCAGAATT CGCTTCTGCC 
AGAGAAAATG CTGGGAACTT CAGCCAGATT GCCTACACTG AAGAGTACGA GCAGCAGAGA 
GGGAAGGGCA GTTTCCCAGC CATGATCACT CCTGCCTACC AGCTTGCCAA GAAAGCCCAG 
GCCCACGCCA GTGATCTGAA ATACAAGAAG GACCTGAATA AGATGAAGGG CTCATCCCAC 
TTCCACAACC TGACCAGTGA GGACAACCTG GCCCTGAAGA GCGCCCGCAA AATCAACAAG 
ATCGTCAGCG AGGTTGAGTA CAAGAAGGAC CTTGAGAACA CTAAAGGCCA CAGCATCAAC 
TACTGTGAGA CGCCCCAGTA CAAGAATGCA TCCAAGGCTG CCAAGTTCAG CAGTGATAAC 
AAGTACAAGG AGAAGTATAC CAACCACATG AAAGGGCACT ATGAAGGCTC TGAGATGGAC 
AAGAATGCTG TACACGCTAT GAACGTCAGA AAGCTGGCAA GCAATATTGC CTACAAGCAG 
GAGTATGAGC AGGAGAAAAC TGACCATGTA GAATACAACT ACCCAGCCAC CATCACACCA 
GGCTACCAGA GTCAGAGGAA ACTGGATCCA CTCAAGGACA AGAACTACAG ACAGCACATT 
GACCAAGTCA AGTACAGCTC GGTGACAGAT ACCCCTGACA TCGTCCAAGC CAGGATCAAC 
GCTCAACAAC TCAGCAATGT TAACTACAGA CAACACATTG ACCAAGTCAA GTACAGCTCG 
GTGACAGATA CCCCTCAAAT CGTCCAGGCC AGGATCAACG CTCAACAACT CAGCAATATA 
AAATACAAGG AGGACTGGGA GAAGACCAAG TCCAAAACAT GTGACATCGG CCTGGACACG 
CTGACCGTCA AGGCTGCCAA AGCATCTCGG GACCTGGCCA GTGATATTAA GTACAAAGAG 
TCATTCATGA AAAATAAAGA TAAGACAAGC AATGTCAGCG ACTCCAAGAC CCTGCACTCT 
CTTCAGGCGT CCAAGATGAC CAGTGAGATT GAGTACAAGA AGAACTCCAA GGAGAACCAG 
TCCCAGTTCA GCTTGTCCAT GGACATGGTC AACCTGAGCC ATGCTAAAAA AGCCCAGGCC 
CTTGCCAGCG ACCTGGACTA CAGGACGAAG CTCCACCACT ACACCATCCT ATCCGACGAC 
ATCCAAGTAC AGCAGGCCAA AAAGGCCTAT AACCTGCAGA GCGAGAATCA ATACCGTTCA 
GATCTGACCT GGATGAAGGG AGTTGGCTGG GAGGCTGATG GATCTCTGAA TGTGACCCAA 
GCCAAGAAGG CTGGAGAGCT TCTCAGTGAC AAAAAGTACC GTCAGAAAGT GGACGACGTC 
AAGTTCACCC AGGTGGCCGA TACTCCCTCT ATCAAACACG CCAAGAAGAG CCAGGAACTG 
CAGAGCAAGC TAACGTATAA GGCGGGCAAC GAACAGATGA TGCACCAGTA CACCATGGAT 
AAAGATGAGC CACTCTTCAG GCAGGCCAAA GCCAATGCAG ACCTCCTCAG TGGGAAAGTG 
TATAAGAGTA GCTGGGAGCA ACAGCGAGAA AAGGGCTTTG AGTTGCGCAT GGACTCCTTG 
TCCATCCTCA CAGCTAAGGC TAAGAGAGAC CTGGCCAGTA ACGTGAAATA CAAGGAGAAG 
TACGAGCAGA CTAAGGGGAA GATGATTGGG GTGAAGATGG TCACTGACGA CTCTCAGATG 
GCGCACTCGG CTCTGGCCAC CCGGTTGCAG AGTGATCGCC ACTACAGAAA GAAGTACGAG 
GACACTAAAA CCAAGCATGG TGCCTCTTTG GACATGTTGA CCATGACCCA CGCCAAGAAG 
GCCCAGGATC TGGCAACTGA GACCAACTAC AGGACCTTCC TGCACAAGTA CACAACCCTG 
CCCGGCGACA TGAAGGTGGC CTGGGCCAAG AAGGCCTACG GACTACTGAG TGATAAACAG 
TACAGGTCGG ATCGGAACTG GATGAAGGGC GTGGGCTGGG TGGCAGCAGA TTTCCTGGAT 
GTGAAACAAG CAAAGAAGGC TGGCGAGCTC GTTAGCGAGA AAAAGTACCG TCAACATGTC 
AGCGATCTGA AGTTCACCAG CGTGGAGGAC ACTCCCGAGA TGGTCCAGGC CAAACTCAGC 
AACAAACTGG CCCTAGACAG GCTGTACAGG GAAAAGGGGG AACATATGAA GCACAACTAC 
ACACTCTCCG GAGATCTGCC TGAACACGTG CAGGCCAAGC TCAATGCCAT GAACATCAGT 
GAGACCCGCT ATAAGGAGTC GTGGACTAAA ATCAGAGATG GTGGCTACAA GCTCCGTTTG 
GATGCCATTC CCTTCCAGTC AGCCAAAGCG TCTGGGAACA TACTTAGTGA TCACAAGTAC 
AAGGAGCAGT TTGAAAAGAC CAAGGGGCAT ATGATTGGAC TGAAGGGACT GGAGAACGAC 
ATGAACATGT CTCACTCTGT GAATGCCAGT AAACTGCAGA GTGACATCAA GTATAAGCAG 
GACTCTGTGA ATAAGCGCTC TCAGTACCAC CTTTCTATGG ACATGTTGGA GATGGCCCAT 
GCTAAAAAGG CCCAGACCCT GGCCAGTGAG CAGGACTACC GGCTTACACT CCACCAGTAC 
ACTACTCAAC CCGATGACAT GAAGGTGCAG GCCGCTAAGA GAGCCTACCA ACTACAGAGC 
GAGAAAGTGT ACCGCTCTGA CCTGAACTAC CTGCGTGGTG TAGCCTGGAT CAGCACCGGG 
GCCCTCCAGA TGGAGGGCTT CAAAAGGGCC ACAGACCTTA TTAGTGAGAA AAACTACCGT 
CAGCAGCCCT GCTCCTTCAA GCACACATCT GTCGCAGACT CACCAGACAT TGTCCATGCC 
AAACTCAGCA ATAAGCTTAC CAACGAGCGC CTGTACAAGA TGAAAGGGGA GAATGACAGG 
CATAACTACA CAATGACATT GGAGAGACCA GAGATAACAC AGGCTAAGAT CAACGCGGCT 
AACTTCAGCG AGACCAAGTA CAGGGAGTCA TGGCACACTC TAAGGGCTCA GGGCTACAAA 
CTGACCATGC AGGACATCCC CTTCCAGGCT GCCAAAACCT CAATGGATTT AGCCAGCGAC 
GTCCAATACA AGCACCACCA CCTGCTGGAT AAGGGCAAGC ACATCGGCGC CCGCAGCATT 
CTGGATGACC CGCGCATGCT ACACTGCATG CAGATGGGCC GGCTGACGAG TGAGCAGGAG 
TACCGCAAAG ACGCACTTTC CACCAGCGGC CAGTACCACC TCAATCCAGA TATGATCAAT 
TTAGTGACAG CCAAGAACGC CCAAGCTCTG GCCAGCGATC AGGACTACAG GAAGAGGCTC 
CATGCGTACA CTGTCCTCCC TGACGATATT AAAGTCCAAT GGGCCAAGAA GGCCTATAAC 
CTGCAGAGCG AGAAACTGTA CAAGTCGGAT CTGAACTTCA TGAAGGGCGT GGCCTGGGAT 
GGTGTGGGTG CGCCTCAAAT GGAGTTGGCA AAGAAAGCCG GAGAACTCAC CAGTAATAAG 
AAATATCGTC AACTGCCAGA CAGTCTGAAG TTCACCCAAG TGGCGGACTC TCCAGACATT 
GTTCATGCTA AGAACAGCTA CCTCCAGTGC AGTGAGAGGC TGTATAAGTC TGGAGACTCG 
GACGCCATGC ACAAATACAC CTTACATGCC GACGACCCAG ACTTCATCAG AGCAAGGATC 
AACGCCCAGC AGATTAGCAA TAAAGCCTAT AAAGCGTCTG GGGATGAGAT GAGGTCGGCA 
GGCTACGACT TGAGGCTGGA TGCTATTCCC TTCCAGACGG CCAAGGCTTC CACGAATATC 
GCCAGTGATT TCCGCTACAA GGAAGCCTTT GTGAAGGAGA AGGGTCAGCA GGTGGGGCTG 
CTCAGTGTCG AAGATGACCC AAAGATGGTG CACTCCCTGG CAGCCAGCAA GCTCCAGAGC 
TGCCTGGGGT ACAAGAGGCA GGCTCAAGAG ACGCGCTCCC AGTTCAAGAT GCATGCCAAC 
CAGCCTGTGT TCCAGCAGGC CAAGAAGAGC CAAGCTCTGG CCAGCAACCA GACCTATCAC 
CAGAGGCTCC ATGACTACAC CTTTGACCCC CAGCAGCTCA ACGTCCAACA CGCCAAGCAG 
GCCTACAAGC TGCAGAGTGA CGTGAACTAC AAGTCAGATC TGAACTGGAT CAGGGGTGTG 
GGCTGGACCC CCCCTGGCTC CCATAAGGTG GAGATGGCCC GGCGGGCTGC AGAACTGGGC 
CTGACGGAGA GCGTGAGCAC TGAGGAGGCC ATTGCCCAGT ACCAACACAT GATGATGATG 
CAGCAGATGG AGGAGCAGCA GCAGCTGTCA GAACCTGTTG AGACCAGCAA AGAGTTCCAG 
CAGTCGGGAG TCAATCTAGA CGCAATGGAG ATCCTCCACG TCAAGAGGAA GAAGACCATC 
CAAACGGTGA AGAAGTCCAA TTCCTCCTCC ATGGATAGCA GCGTCAAGAA GTTAATGATG 
TCTTCCAACT CAGCCATAGA GTCAATGATG GCTTCCACCT CTGTCTCAGC CATTGAGAAC 
ACAGTCAAGA TGTCCACAAC GAGTGAGGCA TCGGCCATAG AAAATGCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nrap ( XM_021575464.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021431139.1 Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X7, mRNA.

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CloneID	OOf25634
Clone ID Related Accession (Same CDS sequence)	XM_021575464.1
Accession Version	XM_021575464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1498406400000
Organism	Oncorhynchus mykiss(rainbow trout)
Product	nebulin-related-anchoring protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035096.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTCCT CTAAAACCCC TGAAGTGCTT CTGTCCAAAA GCCAAGGACA GATCCATTAT 
GTCTACACTG AAGAGTATGA ACAGCAGTTG GGTAAAGGCA GTTTCCCCGC ACATCTCACT 
CCTGGCTACC AAGTTTCAAA GAAAGCCACA GAGATGGCCA GTGGTGTGAA GTACCGTCAG 
ATGTATGAGC AGGAGATGAA GGGAAAGGCC ACCTCAGATG CTGGGGCAGC AGAATTCGCT 
TCTGCCAGAG AAAATGCTGG GAACTTCAGC CAGATTGCCT ACACTGAAGA GTACGAGCAG 
CAGAGAGGGA AGGGCAGTTT CCCAGCCATG ATCACTCCTG CCTACCAGCT TGCCAAGAAA 
GCCCAGGCCC ACGCCAGTGA TCTGAAATAC AAGAAGGACC TGAATAAGAT GAAGGGCTCA 
TCCCACTTCC ACAACCTGAC CAGTGAGGAC AACCTGGCCC TGAAGAGCGC CCGCAAAATC 
AACAAGATCG TCAGCGAGGT TGAGTACAAG AAGGACCTTG AGAACACTAA AGGCCACAGC 
ATCAACTACT GTGAGACGCC CCAGTACAAG AATGCATCCA AGGCTGCCAA GTTCAGCAGT 
GATAACAAGT ACAAGGAGAA GTATACCAAC CACATGAAAG GGCACTATGA AGGCTCTGAG 
ATGGACAAGA ATGCTGTACA CGCTATGAAC GTCAGAAAGC TGGCAAGCAA TATTGCCTAC 
AAGCAGGAGT ATGAGCAGGA GAAAACTGAC CATGTAGAAT ACAACTACCC AGCCACCATC 
ACACCAGGCT ACCAGAGTCA GAGGAAACTG GATCCACTCA AGGACAAGAA CTACAGACAG 
CACATTGACC AAGTCAAGTA CAGCTCGGTG ACAGATACCC CTGACATCGT CCAAGCCAGG 
ATCAACGCTC AACAACTCAG CAATGTTAAC TACAGACAAC ACATTGACCA AGTCAAGTAC 
AGCTCGGTGA CAGATACCCC TCAAATCGTC CAGGCCAGGA TCAACGCTCA ACAACTCAGC 
AATATAAAAT ACAAGGAGGA CTGGGAGAAG ACCAAGTCCA AAACATGTGA CATCGGCCTG 
GACACGCTGA CCGTCAAGGC TGCCAAAGCA TCTCGGGACC TGGCCAGTGA TATTAAGTAC 
AAAGAGTCAT TCATGAAAAA TAAAGATAAG ACAAGCAATG TCAGCGACTC CAAGACCCTG 
CACTCTCTTC AGGCGTCCAA GATGACCAGT GAGATTGAGT ACAAGAAGAA CTCCAAGGAG 
AACCAGTCCC AGTTCAGCTT GTCCATGGAC ATGGTCAACC TGAGCCATGC TAAAAAAGCC 
CAGGCCCTTG CCAGCGACCT GGACTACAGG ACGAAGCTCC ACCACTACAC CATCCTATCC 
GACGACATCC AAGTACAGCA GGCCAAAAAG GCCTATAACC TGCAGAGCGA GAATCAATAC 
CGTTCAGATC TGACCTGGAT GAAGGGAGTT GGCTGGGAGG CTGATGGATC TCTGAATGTG 
ACCCAAGCCA AGAAGGCTGG AGAGCTTCTC AGTGACAAAA AGTACCGTCA GAAAGTGGAC 
GACGTCAAGT TCACCCAGGT GGCCGATACT CCCTCTATCA AACACGCCAA GAAGAGCCAG 
GAACTGCAGA GCAAGCTAAC GTATAAGGCG GGCAACGAAC AGATGATGCA CCAGTACACC 
ATGGATAAAG ATGAGCCACT CTTCAGGCAG GCCAAAGCCA ATGCAGACCT CCTCAGTGGG 
AAAGTGTATA AGAGTAGCTG GGAGCAACAG CGAGAAAAGG GCTTTGAGTT GCGCATGGAC 
TCCTTGTCCA TCCTCACAGC TAAGGCTAAG AGAGACCTGG CCAGTAACGT GAAATACAAG 
GAGAAGTACG AGCAGACTAA GGGGAAGATG ATTGGGGTGA AGATGGTCAC TGACGACTCT 
CAGATGGCGC ACTCGGCTCT GGCCACCCGG TTGCAGAGTG ATCGCCACTA CAGAAAGAAG 
TACGAGGACA CTAAAACCAA GCATGGTGCC TCTTTGGACA TGTTGACCAT GACCCACGCC 
AAGAAGGCCC AGGATCTGGC AACTGAGACC AACTACAGGA CCTTCCTGCA CAAGTACACA 
ACCCTGCCCG GCGACATGAA GGTGGCCTGG GCCAAGAAGG CCTACGGACT ACTGAGTGAT 
AAACAGTACA GGTCGGATCG GAACTGGATG AAGGGCGTGG GCTGGGTGGC AGCAGATTTC 
CTGGATGTGA AACAAGCAAA GAAGGCTGGC GAGCTCGTTA GCGAGAAAAA GTACCGTCAA 
CATGTCAGCG ATCTGAAGTT CACCAGCGTG GAGGACACTC CCGAGATGGT CCAGGCCAAA 
CTCAGCAACA AACTGGCCCT AGACAGGCTG TACAGGGAAA AGGGGGAACA TATGAAGCAC 
AACTACACAC TCTCCGGAGA TCTGCCTGAA CACGTGCAGG CCAAGCTCAA TGCCATGAAC 
ATCAGTGAGA CCCGCTATAA GGAGTCGTGG ACTAAAATCA GAGATGGTGG CTACAAGCTC 
CGTTTGGATG CCATTCCCTT CCAGTCAGCC AAAGCGTCTG GGAACATACT TAGTGATCAC 
AAGTACAAGG AGCAGTTTGA AAAGACCAAG GGGCATATGA TTGGACTGAA GGGACTGGAG 
AACGACATGA ACATGTCTCA CTCTGTGAAT GCCAGTAAAC TGCAGAGTGA CATCAAGTAT 
AAGCAGGACT CTGTGAATAA GCGCTCTCAG TACCACCTTT CTATGGACAT GTTGGAGATG 
GCCCATGCTA AAAAGGCCCA GACCCTGGCC AGTGAGCAGG ACTACCGGCT TACACTCCAC 
CAGTACACTA CTCAACCCGA TGACATGAAG GTGCAGGCCG CTAAGAGAGC CTACCAACTA 
CAGAGCGAGA AAGTGTACCG CTCTGACCTG AACTACCTGC GTGGTGTAGC CTGGATCAGC 
ACCGGGGCCC TCCAGATGGA GGGCTTCAAA AGGGCCACAG ACCTTATTAG TGAGAAAAAC 
TACCGTCAGC AGCCCTGCTC CTTCAAGCAC ACATCTGTCG CAGACTCACC AGACATTGTC 
CATGCCAAAC TCAGCAATAA GCTTACCAAC GAGCGCCTGT ACAAGATGAA AGGGGAGAAT 
GACAGGCATA ACTACACAAT GACATTGGAG AGACCAGAGA TAACACAGGC TAAGATCAAC 
GCGGCTAACT TCAGCGAGAC CAAGTACAGG GAGTCATGGC ACACTCTAAG GGCTCAGGGC 
TACAAACTGA CCATGCAGGA CATCCCCTTC CAGGCTGCCA AAACCTCAAT GGATTTAGCC 
AGCGACGTCC AATACAAGCA CCACCACCTG CTGGATAAGG GCAAGCACAT CGGCGCCCGC 
AGCATTCTGG ATGACCCGCG CATGCTACAC TGCATGCAGA TGGGCCGGCT GACGAGTGAG 
CAGGAGTACC GCAAAGACGC ACTTTCCACC AGCGGCCAGT ACCACCTCAA TCCAGATATG 
ATCAATTTAG TGACAGCCAA GAACGCCCAA GCTCTGGCCA GCGATCAGGA CTACAGGAAG 
AGGCTCCATG CGTACACTGT CCTCCCTGAC GATATTAAAG TCCAATGGGC CAAGAAGGCC 
TATAACCTGC AGAGCGAGAA ACTGTACAAG TCGGATCTGA ACTTCATGAA GGGCGTGGCC 
TGGGATGGTG TGGGTGCGCC TCAAATGGAG TTGGCAAAGA AAGCCGGAGA ACTCACCAGT 
AATAAGAAAT ATCGTCAACT GCCAGACAGT CTGAAGTTCA CCCAAGTGGC GGACTCTCCA 
GACATTGTTC ATGCTAAGAA CAGCTACCTC CAGTGCAGTG AGAGGCTGTA TAAGTCTGGA 
GACTCGGACG CCATGCACAA ATACACCTTA CATGCCGACG ACCCAGACTT CATCAGAGCA 
AGGATCAACG CCCAGCAGAT TAGCAATAAA GCCTATAAAG CGTCTGGGGA TGAGATGAGG 
TCGGCAGGCT ACGACTTGAG GCTGGATGCT ATTCCCTTCC AGACGGCCAA GGCTTCCACG 
AATATCGCCA GTGATTTCCG CTACAAGGAA GCCTTTGTGA AGGAGAAGGG TCAGCAGGTG 
GGGCTGCTCA GTGTCGAAGA TGACCCAAAG ATGGTGCACT CCCTGGCAGC CAGCAAGCTC 
CAGAGCTGCC TGGGGTACAA GAGGCAGGCT CAAGAGACGC GCTCCCAGTT CAAGATGCAT 
GCCAACCAGC CTGTGTTCCA GCAGGCCAAG AAGAGCCAAG CTCTGGCCAG CAACCAGACC 
TATCACCAGA GGCTCCATGA CTACACCTTT GACCCCCAGC AGCTCAACGT CCAACACGCC 
AAGCAGGCCT ACAAGCTGCA GAGTGACGTG AACTACAAGT CAGATCTGAA CTGGATCAGG 
GGTGTGGGCT GGACCCCCCC TGGCTCCCAT AAGGTGGAGA TGGCCCGGCG GGCTGCAGAA 
CTGGGCCTGA CGGAGAGCGT GAGCACTGAG GAGGCCATTG CCCAGTACCA ACACATGATG 
ATGATGCAGC AGATGGAGGA GCAGCAGCAG CTGTCAGAAC CTGTTGAGAC CAGCAAAGAG 
TTCCAGCAGT CGGGAGTCAA TCTAGACGCA ATGGAGATCC TCCACGTCAA GAGGAAGAAG 
ACCATCCAAA CGGTGAAGAA GTCCAATTCC TCCTCCATGG ATAGCAGCGT CAAGAAGTTA 
ATGATGTCTT CCAACTCAGC CATAGAGTCA ATGATGGCTT CCACCTCTGT CTCAGCCATT 
GAGAACACAG TCAAGATGTC CACAACGAGT GAGGCATCGG CCATAGAAAA TGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021431139.1
CDS	26..4822
Translation	MLSSKTPEVLLSKSQGQIHYVYTEEYEQQLGKGSFPAHLTPGYQVSKKATEMASGVKYRQMYEQEMKGKATSDAGAAEFASARENAGNFSQIAYTEEYEQQRGKGSFPAMITPAYQLAKKAQAHASDLKYKKDLNKMKGSSHFHNLTSEDNLALKSARKINKIVSEVEYKKDLENTKGHSINYCETPQYKNASKAAKFSSDNKYKEKYTNHMKGHYEGSEMDKNAVHAMNVRKLASNIAYKQEYEQEKTDHVEYNYPATITPGYQSQRKLDPLKDKNYRQHIDQVKYSSVTDTPDIVQARINAQQLSNVNYRQHIDQVKYSSVTDTPQIVQARINAQQLSNIKYKEDWEKTKSKTCDIGLDTLTVKAAKASRDLASDIKYKESFMKNKDKTSNVSDSKTLHSLQASKMTSEIEYKKNSKENQSQFSLSMDMVNLSHAKKAQALASDLDYRTKLHHYTILSDDIQVQQAKKAYNLQSENQYRSDLTWMKGVGWEADGSLNVTQAKKAGELLSDKKYRQKVDDVKFTQVADTPSIKHAKKSQELQSKLTYKAGNEQMMHQYTMDKDEPLFRQAKANADLLSGKVYKSSWEQQREKGFELRMDSLSILTAKAKRDLASNVKYKEKYEQTKGKMIGVKMVTDDSQMAHSALATRLQSDRHYRKKYEDTKTKHGASLDMLTMTHAKKAQDLATETNYRTFLHKYTTLPGDMKVAWAKKAYGLLSDKQYRSDRNWMKGVGWVAADFLDVKQAKKAGELVSEKKYRQHVSDLKFTSVEDTPEMVQAKLSNKLALDRLYREKGEHMKHNYTLSGDLPEHVQAKLNAMNISETRYKESWTKIRDGGYKLRLDAIPFQSAKASGNILSDHKYKEQFEKTKGHMIGLKGLENDMNMSHSVNASKLQSDIKYKQDSVNKRSQYHLSMDMLEMAHAKKAQTLASEQDYRLTLHQYTTQPDDMKVQAAKRAYQLQSEKVYRSDLNYLRGVAWISTGALQMEGFKRATDLISEKNYRQQPCSFKHTSVADSPDIVHAKLSNKLTNERLYKMKGENDRHNYTMTLERPEITQAKINAANFSETKYRESWHTLRAQGYKLTMQDIPFQAAKTSMDLASDVQYKHHHLLDKGKHIGARSILDDPRMLHCMQMGRLTSEQEYRKDALSTSGQYHLNPDMINLVTAKNAQALASDQDYRKRLHAYTVLPDDIKVQWAKKAYNLQSEKLYKSDLNFMKGVAWDGVGAPQMELAKKAGELTSNKKYRQLPDSLKFTQVADSPDIVHAKNSYLQCSERLYKSGDSDAMHKYTLHADDPDFIRARINAQQISNKAYKASGDEMRSAGYDLRLDAIPFQTAKASTNIASDFRYKEAFVKEKGQQVGLLSVEDDPKMVHSLAASKLQSCLGYKRQAQETRSQFKMHANQPVFQQAKKSQALASNQTYHQRLHDYTFDPQQLNVQHAKQAYKLQSDVNYKSDLNWIRGVGWTPPGSHKVEMARRAAELGLTESVSTEEAIAQYQHMMMMQQMEEQQQLSEPVETSKEFQQSGVNLDAMEILHVKRKKTIQTVKKSNSSSMDSSVKKLMMSSNSAIESMMASTSVSAIENTVKMSTTSEASAIENA

Target ORF information:

RefSeq Version	XM_021575464.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss nebulin related anchoring protein (nrap), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021575464.1

ORF Insert Sequence:

ATGCTTTCCT CTAAAACCCC TGAAGTGCTT CTGTCCAAAA GCCAAGGACA GATCCATTAT 
GTCTACACTG AAGAGTATGA ACAGCAGTTG GGTAAAGGCA GTTTCCCCGC ACATCTCACT 
CCTGGCTACC AAGTTTCAAA GAAAGCCACA GAGATGGCCA GTGGTGTGAA GTACCGTCAG 
ATGTATGAGC AGGAGATGAA GGGAAAGGCC ACCTCAGATG CTGGGGCAGC AGAATTCGCT 
TCTGCCAGAG AAAATGCTGG GAACTTCAGC CAGATTGCCT ACACTGAAGA GTACGAGCAG 
CAGAGAGGGA AGGGCAGTTT CCCAGCCATG ATCACTCCTG CCTACCAGCT TGCCAAGAAA 
GCCCAGGCCC ACGCCAGTGA TCTGAAATAC AAGAAGGACC TGAATAAGAT GAAGGGCTCA 
TCCCACTTCC ACAACCTGAC CAGTGAGGAC AACCTGGCCC TGAAGAGCGC CCGCAAAATC 
AACAAGATCG TCAGCGAGGT TGAGTACAAG AAGGACCTTG AGAACACTAA AGGCCACAGC 
ATCAACTACT GTGAGACGCC CCAGTACAAG AATGCATCCA AGGCTGCCAA GTTCAGCAGT 
GATAACAAGT ACAAGGAGAA GTATACCAAC CACATGAAAG GGCACTATGA AGGCTCTGAG 
ATGGACAAGA ATGCTGTACA CGCTATGAAC GTCAGAAAGC TGGCAAGCAA TATTGCCTAC 
AAGCAGGAGT ATGAGCAGGA GAAAACTGAC CATGTAGAAT ACAACTACCC AGCCACCATC 
ACACCAGGCT ACCAGAGTCA GAGGAAACTG GATCCACTCA AGGACAAGAA CTACAGACAG 
CACATTGACC AAGTCAAGTA CAGCTCGGTG ACAGATACCC CTGACATCGT CCAAGCCAGG 
ATCAACGCTC AACAACTCAG CAATGTTAAC TACAGACAAC ACATTGACCA AGTCAAGTAC 
AGCTCGGTGA CAGATACCCC TCAAATCGTC CAGGCCAGGA TCAACGCTCA ACAACTCAGC 
AATATAAAAT ACAAGGAGGA CTGGGAGAAG ACCAAGTCCA AAACATGTGA CATCGGCCTG 
GACACGCTGA CCGTCAAGGC TGCCAAAGCA TCTCGGGACC TGGCCAGTGA TATTAAGTAC 
AAAGAGTCAT TCATGAAAAA TAAAGATAAG ACAAGCAATG TCAGCGACTC CAAGACCCTG 
CACTCTCTTC AGGCGTCCAA GATGACCAGT GAGATTGAGT ACAAGAAGAA CTCCAAGGAG 
AACCAGTCCC AGTTCAGCTT GTCCATGGAC ATGGTCAACC TGAGCCATGC TAAAAAAGCC 
CAGGCCCTTG CCAGCGACCT GGACTACAGG ACGAAGCTCC ACCACTACAC CATCCTATCC 
GACGACATCC AAGTACAGCA GGCCAAAAAG GCCTATAACC TGCAGAGCGA GAATCAATAC 
CGTTCAGATC TGACCTGGAT GAAGGGAGTT GGCTGGGAGG CTGATGGATC TCTGAATGTG 
ACCCAAGCCA AGAAGGCTGG AGAGCTTCTC AGTGACAAAA AGTACCGTCA GAAAGTGGAC 
GACGTCAAGT TCACCCAGGT GGCCGATACT CCCTCTATCA AACACGCCAA GAAGAGCCAG 
GAACTGCAGA GCAAGCTAAC GTATAAGGCG GGCAACGAAC AGATGATGCA CCAGTACACC 
ATGGATAAAG ATGAGCCACT CTTCAGGCAG GCCAAAGCCA ATGCAGACCT CCTCAGTGGG 
AAAGTGTATA AGAGTAGCTG GGAGCAACAG CGAGAAAAGG GCTTTGAGTT GCGCATGGAC 
TCCTTGTCCA TCCTCACAGC TAAGGCTAAG AGAGACCTGG CCAGTAACGT GAAATACAAG 
GAGAAGTACG AGCAGACTAA GGGGAAGATG ATTGGGGTGA AGATGGTCAC TGACGACTCT 
CAGATGGCGC ACTCGGCTCT GGCCACCCGG TTGCAGAGTG ATCGCCACTA CAGAAAGAAG 
TACGAGGACA CTAAAACCAA GCATGGTGCC TCTTTGGACA TGTTGACCAT GACCCACGCC 
AAGAAGGCCC AGGATCTGGC AACTGAGACC AACTACAGGA CCTTCCTGCA CAAGTACACA 
ACCCTGCCCG GCGACATGAA GGTGGCCTGG GCCAAGAAGG CCTACGGACT ACTGAGTGAT 
AAACAGTACA GGTCGGATCG GAACTGGATG AAGGGCGTGG GCTGGGTGGC AGCAGATTTC 
CTGGATGTGA AACAAGCAAA GAAGGCTGGC GAGCTCGTTA GCGAGAAAAA GTACCGTCAA 
CATGTCAGCG ATCTGAAGTT CACCAGCGTG GAGGACACTC CCGAGATGGT CCAGGCCAAA 
CTCAGCAACA AACTGGCCCT AGACAGGCTG TACAGGGAAA AGGGGGAACA TATGAAGCAC 
AACTACACAC TCTCCGGAGA TCTGCCTGAA CACGTGCAGG CCAAGCTCAA TGCCATGAAC 
ATCAGTGAGA CCCGCTATAA GGAGTCGTGG ACTAAAATCA GAGATGGTGG CTACAAGCTC 
CGTTTGGATG CCATTCCCTT CCAGTCAGCC AAAGCGTCTG GGAACATACT TAGTGATCAC 
AAGTACAAGG AGCAGTTTGA AAAGACCAAG GGGCATATGA TTGGACTGAA GGGACTGGAG 
AACGACATGA ACATGTCTCA CTCTGTGAAT GCCAGTAAAC TGCAGAGTGA CATCAAGTAT 
AAGCAGGACT CTGTGAATAA GCGCTCTCAG TACCACCTTT CTATGGACAT GTTGGAGATG 
GCCCATGCTA AAAAGGCCCA GACCCTGGCC AGTGAGCAGG ACTACCGGCT TACACTCCAC 
CAGTACACTA CTCAACCCGA TGACATGAAG GTGCAGGCCG CTAAGAGAGC CTACCAACTA 
CAGAGCGAGA AAGTGTACCG CTCTGACCTG AACTACCTGC GTGGTGTAGC CTGGATCAGC 
ACCGGGGCCC TCCAGATGGA GGGCTTCAAA AGGGCCACAG ACCTTATTAG TGAGAAAAAC 
TACCGTCAGC AGCCCTGCTC CTTCAAGCAC ACATCTGTCG CAGACTCACC AGACATTGTC 
CATGCCAAAC TCAGCAATAA GCTTACCAAC GAGCGCCTGT ACAAGATGAA AGGGGAGAAT 
GACAGGCATA ACTACACAAT GACATTGGAG AGACCAGAGA TAACACAGGC TAAGATCAAC 
GCGGCTAACT TCAGCGAGAC CAAGTACAGG GAGTCATGGC ACACTCTAAG GGCTCAGGGC 
TACAAACTGA CCATGCAGGA CATCCCCTTC CAGGCTGCCA AAACCTCAAT GGATTTAGCC 
AGCGACGTCC AATACAAGCA CCACCACCTG CTGGATAAGG GCAAGCACAT CGGCGCCCGC 
AGCATTCTGG ATGACCCGCG CATGCTACAC TGCATGCAGA TGGGCCGGCT GACGAGTGAG 
CAGGAGTACC GCAAAGACGC ACTTTCCACC AGCGGCCAGT ACCACCTCAA TCCAGATATG 
ATCAATTTAG TGACAGCCAA GAACGCCCAA GCTCTGGCCA GCGATCAGGA CTACAGGAAG 
AGGCTCCATG CGTACACTGT CCTCCCTGAC GATATTAAAG TCCAATGGGC CAAGAAGGCC 
TATAACCTGC AGAGCGAGAA ACTGTACAAG TCGGATCTGA ACTTCATGAA GGGCGTGGCC 
TGGGATGGTG TGGGTGCGCC TCAAATGGAG TTGGCAAAGA AAGCCGGAGA ACTCACCAGT 
AATAAGAAAT ATCGTCAACT GCCAGACAGT CTGAAGTTCA CCCAAGTGGC GGACTCTCCA 
GACATTGTTC ATGCTAAGAA CAGCTACCTC CAGTGCAGTG AGAGGCTGTA TAAGTCTGGA 
GACTCGGACG CCATGCACAA ATACACCTTA CATGCCGACG ACCCAGACTT CATCAGAGCA 
AGGATCAACG CCCAGCAGAT TAGCAATAAA GCCTATAAAG CGTCTGGGGA TGAGATGAGG 
TCGGCAGGCT ACGACTTGAG GCTGGATGCT ATTCCCTTCC AGACGGCCAA GGCTTCCACG 
AATATCGCCA GTGATTTCCG CTACAAGGAA GCCTTTGTGA AGGAGAAGGG TCAGCAGGTG 
GGGCTGCTCA GTGTCGAAGA TGACCCAAAG ATGGTGCACT CCCTGGCAGC CAGCAAGCTC 
CAGAGCTGCC TGGGGTACAA GAGGCAGGCT CAAGAGACGC GCTCCCAGTT CAAGATGCAT 
GCCAACCAGC CTGTGTTCCA GCAGGCCAAG AAGAGCCAAG CTCTGGCCAG CAACCAGACC 
TATCACCAGA GGCTCCATGA CTACACCTTT GACCCCCAGC AGCTCAACGT CCAACACGCC 
AAGCAGGCCT ACAAGCTGCA GAGTGACGTG AACTACAAGT CAGATCTGAA CTGGATCAGG 
GGTGTGGGCT GGACCCCCCC TGGCTCCCAT AAGGTGGAGA TGGCCCGGCG GGCTGCAGAA 
CTGGGCCTGA CGGAGAGCGT GAGCACTGAG GAGGCCATTG CCCAGTACCA ACACATGATG 
ATGATGCAGC AGATGGAGGA GCAGCAGCAG CTGTCAGAAC CTGTTGAGAC CAGCAAAGAG 
TTCCAGCAGT CGGGAGTCAA TCTAGACGCA ATGGAGATCC TCCACGTCAA GAGGAAGAAG 
ACCATCCAAA CGGTGAAGAA GTCCAATTCC TCCTCCATGG ATAGCAGCGT CAAGAAGTTA 
ATGATGTCTT CCAACTCAGC CATAGAGTCA ATGATGGCTT CCACCTCTGT CTCAGCCATT 
GAGAACACAG TCAAGATGTC CACAACGAGT GAGGCATCGG CCATAGAAAA TGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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nrap cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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