Fam186a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fam186a
Entrez Gene ID	72277
Full Name	RIKEN cDNA 1700030F18 gene
Synonyms	FAM186A
General protein information	Preferred Names RIKEN cDNA 1700030F18 gene Names protein FAM186A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 F1

mRNA and Protein(s)

mRNA	Protein	Name
XM_030248785.1	XP_030104645.1	protein FAM186A isoform X1
XM_030248786.1	XP_030104646.1	protein FAM186A isoform X2
XM_030248787.1	XP_030104647.1	protein FAM186A isoform X3
NM_028180.3	NP_082456.1	protein FAM186A

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A high-resolution anatomical atlas of the transcriptome in the mouse embryo.
Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, Magen A, Canidio E, Pagani M, Peluso I, Lin-Marq N, Koch M, Bilio M, Cantiello I, Verde R, De Masi C, Bianchi SA, Cicchini J, Perroud E, Mehmeti S, Dagand E, Schrinner S, Nürnberger A, Schmidt K, Metz K, Zwingmann C, Brieske N, Springer C, Hernandez AM, Herzog S, Grabbe F, Sieverding C, Fischer B, Schrader K, Brockmeyer M, Dettmer S, Helbig C, Alunni V, Battaini MA, Mura C, Henrichsen CN, Garcia-Lopez R, Echevarria D, Puelles E, Garcia-Calero E, Kruse S, Uhr M, Kauck C, Feng G, Milyaev N, Ong CK, Kumar L, Lam M, Semple CA, Gyenesei A, Mundlos S, Radelof U, Lehrach H, Sarmientos P, Reymond A, Davidson DR, Dollé P, Antonarakis SE, Yaspo ML, Martinez S, Baldock RA, Eichele G, Ballabio A
PLoS biology9(1)e1000582(2011 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fam186a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fam186a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu05264	NM_028180.3 Latest version!	Mus musculus family with sequence similarity 186, member A (Fam186a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu51614	XM_030248785.1 Latest version!	Mus musculus family with sequence similarity 186, member A (Fam186a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu51616	XM_030248786.1 Latest version!	Mus musculus family with sequence similarity 186, member A (Fam186a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMu86795	XM_030248787.1 Latest version!	Mus musculus family with sequence similarity 186, member A (Fam186a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fam186a ( NM_028180.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082456.1 Mus musculus family with sequence similarity 186, member A (Fam186a), mRNA.

Price & Availability↑

CloneID	OMu05264
Clone ID Related Accession (Same CDS sequence)	NM_028180.3
Accession Version	NM_028180.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1550764800000
Organism	Mus musculus(house mouse)
Product	protein FAM186A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC138177.12 and AK006542.1. On Jun 27, 2009 this sequence version replaced NM_028180.2. ##Evidence-Data-START## Transcript exon combination :: BC048613.1, CB273335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGCCTTCGA TCCCCAGCTT TAAGAAGATA CCCGGATTTA CCAAACTTCA GCGGCCGGTC 
TTGGAGTTAT TAATTGATGA CAGCAAAAGA TCTGACCTCT TCAAAACACT CGGACAAGCT 
TCAGTGGAGG CCGTGTGGAA CGCTGACCTG TCTACGTCAA GCTACCCAAT AATAGAGAAG 
GCACCGATGA GTGCGCTCTG GGCCCAGCTG GGTGGGTACC CAGATATTCC CAAGCTGCTG 
CAGTTAGACA TTCAGTCGAC CTTCAGAAAA TCTCTTGCTT CCATCCGGTC ACAGTCTAAG 
AAGATTCGTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082456.1
CDS	204..518
Translation	MPSIPSFKKIPGFTKLQRPVLELLIDDSKRSDLFKTLGQASVEAVWNADLSTSSYPIIEKAPMSALWAQLGGYPDIPKLLQLDIQSTFRKSLASIRSQSKKIRK

Target ORF information:

RefSeq Version	NM_028180.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus family with sequence similarity 186, member A (Fam186a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028180.3

ORF Insert Sequence:

ATGCCTTCGA TCCCCAGCTT TAAGAAGATA CCCGGATTTA CCAAACTTCA GCGGCCGGTC 
TTGGAGTTAT TAATTGATGA CAGCAAAAGA TCTGACCTCT TCAAAACACT CGGACAAGCT 
TCAGTGGAGG CCGTGTGGAA CGCTGACCTG TCTACGTCAA GCTACCCAAT AATAGAGAAG 
GCACCGATGA GTGCGCTCTG GGCCCAGCTG GGTGGGTACC CAGATATTCC CAAGCTGCTG 
CAGTTAGACA TTCAGTCGAC CTTCAGAAAA TCTCTTGCTT CCATCCGGTC ACAGTCTAAG 
AAGATTCGTA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam186a ( XM_030248785.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104645.1 Mus musculus family with sequence similarity 186, member A (Fam186a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu51614
Clone ID Related Accession (Same CDS sequence)	XM_030248785.1
Accession Version	XM_030248785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	protein FAM186A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTCA AAAGGCAGAG CAAAGTCTAC TATGAATCTG ACTCAGATGA TGATGATGAT 
GAATTCAAGG AACTTGCCAC GTTGAGAAAG CCCATAAGCA ACCGTGATAA TCCACTGAGC 
TTGCAATTTG AGATCCCAGC ATCAGTACAG TCAGTCATCC ATAAGATTGA GGAATCACAT 
ATCTTTCGGG CCAAAGAGGA AGTTATTTGG AGGCTGACTG AAATAATGAG CAACGTGGAG 
CTGATAATGA CTCGGTATAA TATCGACAGC ATGTCCCCTG GGAGGAAGGG TTCCAGCTCA 
GAAAGCCAGA AAAAGAAGAG AAAGGCTTTT TTAGAAAAGA TTGCCACAGT TATGACGAAT 
GTTGACCTTA GAGAAAGGAC TCTCTCCAAG ATTCTGTCCT GGCTAGAAGA ATGGAATCTT 
ATTCTGTCCG AGGTGTCTGC CATCAACATG GATGACTACT ACCACTGGAC TGTGAAGATG 
GAGCTGATAC CAGACACGCT GAAAAGGATC AGCAAGAATG TCGACTCTCT GATTCAAATG 
GCCTTATTAC TCGTGGAAGA GAAGAAGAGA GCGAAGAAAA GAATATTGGC TAGAGGCACT 
CTCTGGAAAG CTTGGAAAGA CCGAGCTATA AAGCGGCCTG CCACAGCCCA AGCTTTAAGA 
CTAGATCAGA TGATTTTTGA CCAGATCGGC TTAAACGCTA AGGTTTCAGA GATTCAGGGC 
ATGCTGCAGG AGCTCATCGG CACAGCGATG TTCAGCAAGC TGGAAAATAC TGCCATTAAA 
TACATGTCCA CCACAGTCAT CAATCTCTCC AAGGCCCTGA ACACCGTCAG CGATGAACTA 
AAACTCGTGA GCTCTCGAGT GGTCAAGTCT GTTCTGAAGG AAGAGCAAGA GGAAGATAAG 
GAGAAAGACC GCTCCAAGCA GGTCATCCAA GAACTCAGCG AGGAGAACGA GATGCTCCAG 
CAGAAACTTA GAGAATCCGA AGAAAAATGC GACCAACTTA TTCGCATCAA AAATTATCTT 
GGACGCCAGA TGATACACCC GGCTGGGTCC TTGAAAATAC TGCCTTTACA GGGGCCGTTT 
TTCAGCAGGG AGTCCAGGGA CTCTGAGAGG GAAGGAAAGA CAGACGACTC CCAGAAGGAA 
GGGACCAAAA GCTCCACAGT GACATGGGAC CCATCTTCCA GTGAAGGGGC TCCGGATGCA 
GCTAAGCAGC AAGTTGAGGA CAAAGCTGAG GCCCCCCAAG ACCAGATAAA ACTCAAAGGC 
CTGCCACTGA CAGAGGAGAC CTTGGACACA AATCTGGAAA CGGAGGAAAA GAAACTCACT 
CAACTGGATA TTCTAAAAGC AATGGACAAG AAGAAAGTGG GTAAAGCAAA GGCCGAGCAA 
GGCAGAGGGG GACCAAGTCC CATGTGGGAA CAACTCAAGA AGGGCAGATC TGAGCAGCTG 
ATTCCAGGCA GCACAGGCTC ACCAGAGAGT AGAGAGTCCA CCCTTAAATT AACAGACAAA 
GAAGCCAAGA GTGAAATAGA TCTGCTTCAG CCTGAGGGTA CCCCAGAGCT ACAAAAACCA 
GAAACAAAGT GGAAAAGACT CATAGCAAAA ACTGAAGAAA CAAAACGGTC TGGTAAGTTA 
CAACCCAAGA CTTCTAAGGT GACTTCAGTA ACCCCTGATG AAGGTGAGCA GGGCAGTCTA 
GAATCATTTC AAAGAGCCAT CCTGGCCTTC CTGAGAGAGA AGACGAATAA TGTAGGAAAG 
GCTTTTGATC CAAAGAGTAT ACGGAAGGAG GAGTCGTCAC TGGGAAAAGC AGAAGTTGAA 
AATTTAAACG TCATAAAGGC AAAAATAGAG GAATATTTCC AAAAAGTGGC TGAAACTGTG 
ACTGTAGCCT TGAGGACATA CAGAGATGAA AGAAAGGTTC AACCTAAAGA GAAGCCCAAG 
AAACAGCCAA AGGGAGCACT GTCACGGATG CTTCCTAAGC AGGGGGCGGT CGGCGCCAAG 
TCTGAGGTTG GCAGAGTTCT CCTCAGTGAG ATCACAGACC CAGCAATTAA GAAACTGGTA 
CAGATGCTCT TAAATGAGAT AGAGAGTGAC TTCAAATACA AAACGACGAA GGAACAGCCA 
CAGGCGGCGG TGGCGGCAGC GGCAGCGGCA GCGGCTGTGC AGAAGGAAGA GCCAGGACTG 
AGGACGAAGG ACAAAACGGA GCTGCTGGAG GTGTGGATGC AAGCATTCAA AGACTCACAA 
GGCCACCAGG TTCAGGACCT GGGAGATGCA GAGGATGCAG AAAAGCCGAA GTGGAGCGAG 
CCGGGTCATC AGGACCTGTG GAAGGGTGTC AGCAAGACCT CCATCAATCT AACTCCCAGG 
CTGATGAAGG CCCCGAAACC GCAGATAAGT CAGGCGCAGG CGCTCCAGGT GGAGCCTGGT 
GTAATGGGGG CCACGGAGGA ACAGCAGCCC AAACTCACTC TCTCCTCTTC CAGAATCCCC 
TTCGTTTCTC CAAAGCCCCA CCAAGTGCAT GTCCCTGAGC AGGCCCAGAT AATAGGCCCT 
CAGATGTCCC CTCAGCTTTC AATGGCATCT CTCACCTCTG AGCAGGCACA AACTTTGAGT 
GGTCCTCTTA CTCATGAACA CACTATAGAA TTAAAGACTG CTTTCACAGC AGGGCAGGAG 
CAAGAGCTTG GGGCTAAGCT GATGGTTCCT AGGATTCTTA AGCCGACACA GACTCCAAAG 
GAGTCTCTCA TATCAGAAAA ACCTGAGGCA GTGCAGATCT CCCAAGTGAC TACTGAAGAG 
ATATCTGGAG TCACAGATAC CCAAAGAACA ATTCCACCCT TCCAGCAGAT CCTGGAACAA 
GAAACCACTC CCACTCCTGA GCAAGACAAG GAACCAAGGA TTACTCTCAC CCGTCAGCAG 
ACTGAGGCCC TAGGTATCAC TCTCACCCCT GAGCAAACTA AGTCCCAGAG GATTAGTCTA 
ACCCATGAGC AGGCTCGGGC CCTAGGGGTC ACTCTCACTC CTGAGCAGTA CAAAGAGGAC 
AGAATCAGTC TGACTCCCCT ACAGGCCCAG GTCCTGGGAA TCACTCTCAA CCTTCAGCAG 
GCTAAGGCCC TGGGGATCAC TCTCACTCCT GAGCAAGTCA AGGCACAGAG GGTCAATCTG 
ATCCCTCAAC AGTACCAGGT CCACGGAACC ACTCTCACCA CTCAGCAGGC TGAAGCACAA 
AGAACTAATC TCACGCCCGA GCAAGCCAAG GCCCTGGGTC TCCCTCTGAT CCCTCCTAAG 
CCAATCACTT TCACCCGTGA GCAGACCCAG GCCCTGGGTA TCACTCCAAC CCATCAGCCA 
ATCACTCTCA CCTCTGAGCA GGTCCAGGCC CTGGGTATCA CTCCAACCCA TCAGCCAATC 
ACTCTCACCC CTGAGCAGGC CCAGGCCCTG GCACTCATTC TCACCACTGA GCAGGTCAAG 
ACTCAGAGAA TCAATCTGAG CCCTGATCAG ACCCAGGCCC TGGGTATCAC CCCGACCCCT 
CAGCCGATCA CTTTCACCCC TGAGCAGACC CAGGCTCTGG GTATCACCCC AACCCCTCAG 
CTGATCACAC TAACCCCTGA GCAGGCCAAG GCCTTGGCGA ACACTCTCAC CGCTGAGCAG 
GTCAGTCTGA GCCCTCAACA GGCTGAGGCC CTGGGTATCA CTCCGACCCC TCAGCCGACC 
ACTCTCACCC CTGAGCAGGC CCAGGCCCTG GGTATCACTC CTACCCCACA GCCGATCACA 
CTAACCCCTG AGCAGGCCCA GGCCCTGGGC ATCACTCCGA CCCCACAGCC GACCACACTA 
ACCCCTGAGC AGACCCAGGC CCTGGGTATC ACTCCTACCC CACAGCCGAT CACACTAACC 
CCTGAGCAGG CCCAGGCCCT GGGCATCACT CCGACCCCTC AGCCGATCAC ACTAACCCCT 
GAGCAGACCC AGGCCCTGGG TATCACTCCT ACCCCACAGC CGATCACACT AACCCCTGAG 
CAGGCCCAGG CCCTGGGCAT CACTCCGACC CCTCAGCCGA TCACACTAAC CCCTGAGCAG 
ACCCAGGCCC TGGGTATCAC TCCTACCCCA CAGCCGATCA CACTAACCCC TGAGCAGGCC 
CAGGCCCTGG GCATCACTCC GACCCCTCAG CCGATCACAC TAACCCCTGA GCAGGCCCAG 
GCCCTGGGCA TCACTCCGAC CCCTCAGCCG ATCACACTAA CCCCTGAGCA GGCCCAGGCC 
CTGGGCATCA CTCCGACCCC TCAGCCGATC ACACTAACCC CTGAGCAGAC CCAGGCCCTG 
GGCATCACTC CGACCCCTCA GCCGATCACA CTAACCCCTG AGCAGGCCCA GGCCCTGGGC 
ATCACTCCGA CCCCTCAGCC GATCACACTA ACCCCTGAGC AGGTCCAGGC CCTGGGCATC 
ACTCCGACCC CTCAGCCGAT CACACTAACC CCTGAGCAGG CCCAGGCCCT GGGTATCACT 
CCTACCCCAC AGCCGATCAC ACTAACCCCT GAGCAGGCCC AGGCCCTGGG CATCACTCCG 
ACCCCTCAGC CGATCACACT AACCCCTGAG CAGACCCAGG CCCTGGGTAT CACTCCTACC 
CCACAGCCGA TCACACTAAC CCCTGAGCAG GCCCAGGCCC TGGGCATCAC TCCTACCCCT 
CAGCCGATCA CACTAACCCC TGAGCTGGTC CAGGCCCTGG GCATCACTCC GACCCCTCAG 
CCGATCACAC TAACCCCTGA GCAGGCCCAG GCCCTGGGCA TCACTCCGAC CCCTCAGCCG 
ACCACTCTCA GCCCTGAACA GGCCCAGGCC CTGGGTATCA CTCCGACCCC TCAGCCGATC 
ACACTAACCC CTGAGCAGGC CCAGGCCCTG GGCATCACTC CGACCCCTCA GCCGACCACT 
CTCAGCCCTG AACAGGCCCA GGCTCTGGGG ATTTCTCTCA TTCCTAAACA ACAGGAGATC 
AGTCTTAGTC CAGAGCAGGC CCAAGCTCTG GGGCTCACTC TCACCCCTCA GCAGGCCCAG 
GTACAAAAGA TATATCTCAC TCCTCAGCAG GCCCAGGCTC TGGGAATCAC TGTCAGCCCT 
GAGCAGGCCA AGGCCAAGGG GATCAGTCTG ACCCCTGAGG AGGCCCACTC CCTAGGGATA 
ATTCTCACAG TTGAGCAGGC CAAGGCAAAG AGGATTAATT TGACCCCTCA ACAAGCTCAG 
GACCTGGGGC TCACTCTCAC CCCTGAGCAG GCCCAGGATC TGGGGATTTC TCTCATTCCC 
AAACAAGAGA TCAGTTTTAG TCCCCTGCAG GCCCAAGCAA TGGGGCTCAC TCTCACCCCT 
CAGCAGGCCC AGGTACAAAA GATATATCTC ACTCCTCAGC AGGCCCAGGC TCTGGGAATC 
ACTGTCAGCC CTGAGCAGGC AAAGGGGATC AGTCTGACCC CTGAGGAGGC CCACTCCCTA 
GGGATAATTC TCACAGTTGA GCAGGCCAAG GCACAGAGGA TTAATTTGAC CCCTCAACAA 
GCTCAGGACC TGGGGCTCAC TCTCACCCCT GAGCAGGCCC AGGATCTGGG GATTTCTCTC 
ATTCCCAAAC AACAAGAGAT CAGTTTTAGT CCCCTGCAGG CCCAAGCAAT GGGGCTCACT 
CTCACCCCTC AGCAGGCCCA GGTACAAAAG ATATATCTCA CTCCTCAGCA GGCCCAGGCT 
CTGGGAATCA CTGTCAGCCC TGAGCAGGCA AAGGGGATCA GTCTGACCCC TGAGGAGGCC 
CACTCCCTAG GGATAATTCT CACAGTTGAG CAGGCCAAGG CACAGAGGAT TAATTTGACC 
CCTCAACAAG CTCAGGACCT GGGGATCACT CTCACCCCTG AGCAGGCCCA GGATCTGGGG 
ATTTCTCTCA TTCCCAAACA ACAAGAGATC AGTTTTAGTC CCCTGCAGGC CCAAGCAATG 
GGGCTCACTC TCACCCCTCA GCAGGCCCAG GTACAAAAGA TATATCTCAC TCCTCAGCAG 
GCCCAGGCTC TGGGAATCAC TGTCAGCCCT GAGCAGGCAA AGGGGATCAG TCTGACCCCT 
GAGGAGGCCC ACTCCCTGGG AATCGCTCTC ACAGTTGAGC AGGCCAAGGC ACAGAGGATT 
AATCTGACCC CTCAGCAAGC TCAGGACCTG GGGCTCACTC TCACCCCTGA GCAGGCCCAG 
GATCTGGGAA TAGCTTCTAC CCTTAAGCAG GCTGAAGCTG TGGGAATCAT TCCCACCCCT 
ACCCCTGAGC CTTACCAGGA AAGACGTTTA AGTCTGACCT CTGAGCAGGT TCAGGCCTTG 
AGGACCAGTT TCCCCACTAA GGAGACCCTG TCATTGGGGA TTTATCTCAC CCCTAAGCAG 
GCCCAGTACC TGGGAATCAC TCTCACTGCT AGTCAAGCCA AGGTAATGAA GATTTGCCTC 
ACCCCTGAGC AGGCCCAGGC CCTGGGGATC ACTGTCACCC CTAAGCAGGC CAAGGCACGG 
AGGACCAGTC TGACCCCTGA GCAGGCCCAG GCTCTGGGAG TCATTCTTAC TCCTGAACAG 
GCCCAGGCCC ACAGAATCAC AGTCACTCCT GAGCAGGCCC AGGCTCTGGG GATTGCTCTC 
ACACCTGAGC AGGCCCATGC CCTGGGGATT GCTCTCACAC CTGAGCAGGC CCAGGCACAA 
GGTATTTTTT CTCCTAAGAA ATTCCAGGAA TTGGATGTTC CATTAACCTA TAGACAAACA 
AAAGCACTAA GGAGCCATCT TTCTAAGAAG CAACTTGAAG CCCAGAGAAG TCAAATCCTT 
ATCTCTGAGT CTATCCAAAT CCCCAAACCA GCTGTCGCCT CTAAATATAC CCAGACAATT 
GAAATCCCAC TAACTACCCA ACCATTCCAG GCATTTGGAG CTCTACTTTC AAAGACAAAG 
GAGACAGAAA TGGGAATCTC TCCCCTCACT CCCAAGCAGC CCCAGGCAAT TGTAACTCCA 
CCTTCAAAGA GAAAGGAGAA AGAATTGGGA ATCTCTCCCC TCACTCCCAA GCAGCCCCAG 
GCAGTTGAGC CTGCCAAGGC AAAGCTGCCA CCCCTCACAC CCAGTCAGGC CCAGCCTTTG 
CAGAAGCAGC TTGCTCCAGA GCTCACACAA ACACTGCTGT TCACCATCAC TCTCCAGAAA 
GCCCAGCACT TGGGTGTCAC TTTTACCTAC GAACAAACTC AGGCAGCAGC TGTCACCCTC 
ACCTCTGAGC AGGTGGCAGC ACTAGAAGAC GCTCTCACTG AGAATCTGGC TTGGAGATGG 
GAGATTTCAG TCACACCAGG AATGGCTCAA GAGGCACCAA ACATCACTAC CACTAAGCAA 
CTGCAAGCCC TGGGGATTAC TGCTCGCCAG CCAGCTCAGG CATTTCCCAG CCCTTTTACC 
TTGGAGAAGC CTGCCACGTT GGCTACTTCC ACCGACAGAC TATCTCAAAG ATGGAAGGAT 
TCTTACCCTG CTTCTATTCC ACTGCAGGCA TTGAGGCCTT CTCCTACTCA AGCCCCATTT 
ACCCCTACGA CAAGCCTAGG AATAGGTAGT CTTCTGGACT CTGAGAAGCC ATGGATGTCT 
CCTACCTACA GACAGACTCT CACAGACAGG GGCCAAGACG TTCTTGCACA GCCCCTAGCA 
CCAGAAACCC CTCCTTCCCT CAGACAGCTC CTAGCACCTG GGGCTCCTCC CACTCCAGGG 
CCACCCCTTG GTCCTAGGCA TTTCTTCAAA CCTAGAGTCC CACCCACCTC TGGAGAGGTG 
CCAGGACTTG TTTCTGGAGG CTCTGCTGCC CATGAGGAGC TCCCAATGTC CAGAACCACC 
CCCCTCCAGC CTCCTGAATG GCAAGGCCCA TCAAGGCTCA TTCCCGAGCA AGGCTTCATG 
CCAGCAATCT CTTCCATCCC ACTGCATCCT TTCACAGCAG AGGCCCTACC AACCCCTGGA 
AGACCTCAGA GAAGTTCAAA AGCCAAACCT CTAAAACCTA AATCAGCAAG AGGTCTCCCA 
AATGTCACTC TGGGTTTTGA GACCTCTCAG GCTCCATTCC CTATTGAGAA GACCCAAATA 
CCTAAGACTC CTGACACTTC AGAACAAACT CAAGCACTCC AAGATGCCTT GGGTGTGCAA 
CCATTTGGAA TATTTCAGCC TTATGGCACC AGCTCCGGGA TAGCTCGGTC ACAGTCCCCT 
CTCATTGATG AGAAAGCCCT TTCTAGAGAG AAGCCTGGAA CACCACTACC ATCTCTTACC 
ACTCAACTCC CCCAAACACC ACAGATCTCA ACTTCTGAAA AGGGCCAGAA ACCATGGCTC 
CCGCCGATAG ACAAGCCCTG GACACCAACC CCTGTTTCTA GTACCAGAGA AGCCAAGATG 
ATAGTGTCCC CCACTGATCA GCACCCTGAA GACGGCTATG TCGTCGATGT GGAGGCTCAG 
AGGAAGAACC TGGTCACATT AAATCAGGCG GCACAAACTT CTGCACTCCC TGCACAGTAT 
CTCACAATTG CCAAGAATCT CATAATCGAA TTACTTCATA TAGATACAGT TCGGCTGGGG 
TATCTGTCCC GAAAGTATGT TGCCTATAGG CTGATCCAGC TTGCCAGAAA CCACCTAACC 
AAACGAGTGA AAACCATCCA AAACACTGGG AAAGGGTACG AGGTTCAGAG CTTGTACACG 
ATGCTTGATA GGATTGACCA GTACCAGAAG AAGGTGATGC ACAGCTGGAC GGACAAGCAG 
AAGCAGCTGG AACAGAGACG AAAGCAGTGC CTACGGTCTA TGACGCAGTT CTTCAGCCAG 
CTTGAAAGGG GATATAAACT CAGTCTTAGC CAGCCCATGC CTTCGATCCC CAGCTTTAAG 
AAGATACCCG GATTTACCAA ACTTCAGCGG CCGGTCTTGG AGTTATTAAT TGATGACAGC 
AAAAGATCTG ACCTCTTCAA AACACTCGGA CAAGCTTCAG TGGAGGCCGT GTGGAACGCT 
GACCTGTCTA CGTCAAGCTA CCCAATAATA GAGAAGGCAC CGATGAGTGC GCTCTGGGCC 
CAGCTGGGTG GGTACCCAGA TATTCCCAAG CTGCTGCAGT TAGACATTCA GTCGACCTTC 
AGAAAATCTC TTGCTTCCAT CCGGTCACAG TCTAAGAAGA TTCGTAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104645.1
CDS	147..9197
Translation	MPVKRQSKVYYESDSDDDDDEFKELATLRKPISNRDNPLSLQFEIPASVQSVIHKIEESHIFRAKEEVIWRLTEIMSNVELIMTRYNIDSMSPGRKGSSSESQKKKRKAFLEKIATVMTNVDLRERTLSKILSWLEEWNLILSEVSAINMDDYYHWTVKMELIPDTLKRISKNVDSLIQMALLLVEEKKRAKKRILARGTLWKAWKDRAIKRPATAQALRLDQMIFDQIGLNAKVSEIQGMLQELIGTAMFSKLENTAIKYMSTTVINLSKALNTVSDELKLVSSRVVKSVLKEEQEEDKEKDRSKQVIQELSEENEMLQQKLRESEEKCDQLIRIKNYLGRQMIHPAGSLKILPLQGPFFSRESRDSEREGKTDDSQKEGTKSSTVTWDPSSSEGAPDAAKQQVEDKAEAPQDQIKLKGLPLTEETLDTNLETEEKKLTQLDILKAMDKKKVGKAKAEQGRGGPSPMWEQLKKGRSEQLIPGSTGSPESRESTLKLTDKEAKSEIDLLQPEGTPELQKPETKWKRLIAKTEETKRSGKLQPKTSKVTSVTPDEGEQGSLESFQRAILAFLREKTNNVGKAFDPKSIRKEESSLGKAEVENLNVIKAKIEEYFQKVAETVTVALRTYRDERKVQPKEKPKKQPKGALSRMLPKQGAVGAKSEVGRVLLSEITDPAIKKLVQMLLNEIESDFKYKTTKEQPQAAVAAAAAAAAVQKEEPGLRTKDKTELLEVWMQAFKDSQGHQVQDLGDAEDAEKPKWSEPGHQDLWKGVSKTSINLTPRLMKAPKPQISQAQALQVEPGVMGATEEQQPKLTLSSSRIPFVSPKPHQVHVPEQAQIIGPQMSPQLSMASLTSEQAQTLSGPLTHEHTIELKTAFTAGQEQELGAKLMVPRILKPTQTPKESLISEKPEAVQISQVTTEEISGVTDTQRTIPPFQQILEQETTPTPEQDKEPRITLTRQQTEALGITLTPEQTKSQRISLTHEQARALGVTLTPEQYKEDRISLTPLQAQVLGITLNLQQAKALGITLTPEQVKAQRVNLIPQQYQVHGTTLTTQQAEAQRTNLTPEQAKALGLPLIPPKPITFTREQTQALGITPTHQPITLTSEQVQALGITPTHQPITLTPEQAQALALILTTEQVKTQRINLSPDQTQALGITPTPQPITFTPEQTQALGITPTPQLITLTPEQAKALANTLTAEQVSLSPQQAEALGITPTPQPTTLTPEQAQALGITPTPQPITLTPEQAQALGITPTPQPTTLTPEQTQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQTQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQTQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQTQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQVQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQTQALGITPTPQPITLTPEQAQALGITPTPQPITLTPELVQALGITPTPQPITLTPEQAQALGITPTPQPTTLSPEQAQALGITPTPQPITLTPEQAQALGITPTPQPTTLSPEQAQALGISLIPKQQEISLSPEQAQALGLTLTPQQAQVQKIYLTPQQAQALGITVSPEQAKAKGISLTPEEAHSLGIILTVEQAKAKRINLTPQQAQDLGLTLTPEQAQDLGISLIPKQEISFSPLQAQAMGLTLTPQQAQVQKIYLTPQQAQALGITVSPEQAKGISLTPEEAHSLGIILTVEQAKAQRINLTPQQAQDLGLTLTPEQAQDLGISLIPKQQEISFSPLQAQAMGLTLTPQQAQVQKIYLTPQQAQALGITVSPEQAKGISLTPEEAHSLGIILTVEQAKAQRINLTPQQAQDLGITLTPEQAQDLGISLIPKQQEISFSPLQAQAMGLTLTPQQAQVQKIYLTPQQAQALGITVSPEQAKGISLTPEEAHSLGIALTVEQAKAQRINLTPQQAQDLGLTLTPEQAQDLGIASTLKQAEAVGIIPTPTPEPYQERRLSLTSEQVQALRTSFPTKETLSLGIYLTPKQAQYLGITLTASQAKVMKICLTPEQAQALGITVTPKQAKARRTSLTPEQAQALGVILTPEQAQAHRITVTPEQAQALGIALTPEQAHALGIALTPEQAQAQGIFSPKKFQELDVPLTYRQTKALRSHLSKKQLEAQRSQILISESIQIPKPAVASKYTQTIEIPLTTQPFQAFGALLSKTKETEMGISPLTPKQPQAIVTPPSKRKEKELGISPLTPKQPQAVEPAKAKLPPLTPSQAQPLQKQLAPELTQTLLFTITLQKAQHLGVTFTYEQTQAAAVTLTSEQVAALEDALTENLAWRWEISVTPGMAQEAPNITTTKQLQALGITARQPAQAFPSPFTLEKPATLATSTDRLSQRWKDSYPASIPLQALRPSPTQAPFTPTTSLGIGSLLDSEKPWMSPTYRQTLTDRGQDVLAQPLAPETPPSLRQLLAPGAPPTPGPPLGPRHFFKPRVPPTSGEVPGLVSGGSAAHEELPMSRTTPLQPPEWQGPSRLIPEQGFMPAISSIPLHPFTAEALPTPGRPQRSSKAKPLKPKSARGLPNVTLGFETSQAPFPIEKTQIPKTPDTSEQTQALQDALGVQPFGIFQPYGTSSGIARSQSPLIDEKALSREKPGTPLPSLTTQLPQTPQISTSEKGQKPWLPPIDKPWTPTPVSSTREAKMIVSPTDQHPEDGYVVDVEAQRKNLVTLNQAAQTSALPAQYLTIAKNLIIELLHIDTVRLGYLSRKYVAYRLIQLARNHLTKRVKTIQNTGKGYEVQSLYTMLDRIDQYQKKVMHSWTDKQKQLEQRRKQCLRSMTQFFSQLERGYKLSLSQPMPSIPSFKKIPGFTKLQRPVLELLIDDSKRSDLFKTLGQASVEAVWNADLSTSSYPIIEKAPMSALWAQLGGYPDIPKLLQLDIQSTFRKSLASIRSQSKKIRK

Target ORF information:

RefSeq Version	XM_030248785.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus family with sequence similarity 186, member A (Fam186a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248785.1

ORF Insert Sequence:

ATGCCTGTCA AAAGGCAGAG CAAAGTCTAC TATGAATCTG ACTCAGATGA TGATGATGAT 
GAATTCAAGG AACTTGCCAC GTTGAGAAAG CCCATAAGCA ACCGTGATAA TCCACTGAGC 
TTGCAATTTG AGATCCCAGC ATCAGTACAG TCAGTCATCC ATAAGATTGA GGAATCACAT 
ATCTTTCGGG CCAAAGAGGA AGTTATTTGG AGGCTGACTG AAATAATGAG CAACGTGGAG 
CTGATAATGA CTCGGTATAA TATCGACAGC ATGTCCCCTG GGAGGAAGGG TTCCAGCTCA 
GAAAGCCAGA AAAAGAAGAG AAAGGCTTTT TTAGAAAAGA TTGCCACAGT TATGACGAAT 
GTTGACCTTA GAGAAAGGAC TCTCTCCAAG ATTCTGTCCT GGCTAGAAGA ATGGAATCTT 
ATTCTGTCCG AGGTGTCTGC CATCAACATG GATGACTACT ACCACTGGAC TGTGAAGATG 
GAGCTGATAC CAGACACGCT GAAAAGGATC AGCAAGAATG TCGACTCTCT GATTCAAATG 
GCCTTATTAC TCGTGGAAGA GAAGAAGAGA GCGAAGAAAA GAATATTGGC TAGAGGCACT 
CTCTGGAAAG CTTGGAAAGA CCGAGCTATA AAGCGGCCTG CCACAGCCCA AGCTTTAAGA 
CTAGATCAGA TGATTTTTGA CCAGATCGGC TTAAACGCTA AGGTTTCAGA GATTCAGGGC 
ATGCTGCAGG AGCTCATCGG CACAGCGATG TTCAGCAAGC TGGAAAATAC TGCCATTAAA 
TACATGTCCA CCACAGTCAT CAATCTCTCC AAGGCCCTGA ACACCGTCAG CGATGAACTA 
AAACTCGTGA GCTCTCGAGT GGTCAAGTCT GTTCTGAAGG AAGAGCAAGA GGAAGATAAG 
GAGAAAGACC GCTCCAAGCA GGTCATCCAA GAACTCAGCG AGGAGAACGA GATGCTCCAG 
CAGAAACTTA GAGAATCCGA AGAAAAATGC GACCAACTTA TTCGCATCAA AAATTATCTT 
GGACGCCAGA TGATACACCC GGCTGGGTCC TTGAAAATAC TGCCTTTACA GGGGCCGTTT 
TTCAGCAGGG AGTCCAGGGA CTCTGAGAGG GAAGGAAAGA CAGACGACTC CCAGAAGGAA 
GGGACCAAAA GCTCCACAGT GACATGGGAC CCATCTTCCA GTGAAGGGGC TCCGGATGCA 
GCTAAGCAGC AAGTTGAGGA CAAAGCTGAG GCCCCCCAAG ACCAGATAAA ACTCAAAGGC 
CTGCCACTGA CAGAGGAGAC CTTGGACACA AATCTGGAAA CGGAGGAAAA GAAACTCACT 
CAACTGGATA TTCTAAAAGC AATGGACAAG AAGAAAGTGG GTAAAGCAAA GGCCGAGCAA 
GGCAGAGGGG GACCAAGTCC CATGTGGGAA CAACTCAAGA AGGGCAGATC TGAGCAGCTG 
ATTCCAGGCA GCACAGGCTC ACCAGAGAGT AGAGAGTCCA CCCTTAAATT AACAGACAAA 
GAAGCCAAGA GTGAAATAGA TCTGCTTCAG CCTGAGGGTA CCCCAGAGCT ACAAAAACCA 
GAAACAAAGT GGAAAAGACT CATAGCAAAA ACTGAAGAAA CAAAACGGTC TGGTAAGTTA 
CAACCCAAGA CTTCTAAGGT GACTTCAGTA ACCCCTGATG AAGGTGAGCA GGGCAGTCTA 
GAATCATTTC AAAGAGCCAT CCTGGCCTTC CTGAGAGAGA AGACGAATAA TGTAGGAAAG 
GCTTTTGATC CAAAGAGTAT ACGGAAGGAG GAGTCGTCAC TGGGAAAAGC AGAAGTTGAA 
AATTTAAACG TCATAAAGGC AAAAATAGAG GAATATTTCC AAAAAGTGGC TGAAACTGTG 
ACTGTAGCCT TGAGGACATA CAGAGATGAA AGAAAGGTTC AACCTAAAGA GAAGCCCAAG 
AAACAGCCAA AGGGAGCACT GTCACGGATG CTTCCTAAGC AGGGGGCGGT CGGCGCCAAG 
TCTGAGGTTG GCAGAGTTCT CCTCAGTGAG ATCACAGACC CAGCAATTAA GAAACTGGTA 
CAGATGCTCT TAAATGAGAT AGAGAGTGAC TTCAAATACA AAACGACGAA GGAACAGCCA 
CAGGCGGCGG TGGCGGCAGC GGCAGCGGCA GCGGCTGTGC AGAAGGAAGA GCCAGGACTG 
AGGACGAAGG ACAAAACGGA GCTGCTGGAG GTGTGGATGC AAGCATTCAA AGACTCACAA 
GGCCACCAGG TTCAGGACCT GGGAGATGCA GAGGATGCAG AAAAGCCGAA GTGGAGCGAG 
CCGGGTCATC AGGACCTGTG GAAGGGTGTC AGCAAGACCT CCATCAATCT AACTCCCAGG 
CTGATGAAGG CCCCGAAACC GCAGATAAGT CAGGCGCAGG CGCTCCAGGT GGAGCCTGGT 
GTAATGGGGG CCACGGAGGA ACAGCAGCCC AAACTCACTC TCTCCTCTTC CAGAATCCCC 
TTCGTTTCTC CAAAGCCCCA CCAAGTGCAT GTCCCTGAGC AGGCCCAGAT AATAGGCCCT 
CAGATGTCCC CTCAGCTTTC AATGGCATCT CTCACCTCTG AGCAGGCACA AACTTTGAGT 
GGTCCTCTTA CTCATGAACA CACTATAGAA TTAAAGACTG CTTTCACAGC AGGGCAGGAG 
CAAGAGCTTG GGGCTAAGCT GATGGTTCCT AGGATTCTTA AGCCGACACA GACTCCAAAG 
GAGTCTCTCA TATCAGAAAA ACCTGAGGCA GTGCAGATCT CCCAAGTGAC TACTGAAGAG 
ATATCTGGAG TCACAGATAC CCAAAGAACA ATTCCACCCT TCCAGCAGAT CCTGGAACAA 
GAAACCACTC CCACTCCTGA GCAAGACAAG GAACCAAGGA TTACTCTCAC CCGTCAGCAG 
ACTGAGGCCC TAGGTATCAC TCTCACCCCT GAGCAAACTA AGTCCCAGAG GATTAGTCTA 
ACCCATGAGC AGGCTCGGGC CCTAGGGGTC ACTCTCACTC CTGAGCAGTA CAAAGAGGAC 
AGAATCAGTC TGACTCCCCT ACAGGCCCAG GTCCTGGGAA TCACTCTCAA CCTTCAGCAG 
GCTAAGGCCC TGGGGATCAC TCTCACTCCT GAGCAAGTCA AGGCACAGAG GGTCAATCTG 
ATCCCTCAAC AGTACCAGGT CCACGGAACC ACTCTCACCA CTCAGCAGGC TGAAGCACAA 
AGAACTAATC TCACGCCCGA GCAAGCCAAG GCCCTGGGTC TCCCTCTGAT CCCTCCTAAG 
CCAATCACTT TCACCCGTGA GCAGACCCAG GCCCTGGGTA TCACTCCAAC CCATCAGCCA 
ATCACTCTCA CCTCTGAGCA GGTCCAGGCC CTGGGTATCA CTCCAACCCA TCAGCCAATC 
ACTCTCACCC CTGAGCAGGC CCAGGCCCTG GCACTCATTC TCACCACTGA GCAGGTCAAG 
ACTCAGAGAA TCAATCTGAG CCCTGATCAG ACCCAGGCCC TGGGTATCAC CCCGACCCCT 
CAGCCGATCA CTTTCACCCC TGAGCAGACC CAGGCTCTGG GTATCACCCC AACCCCTCAG 
CTGATCACAC TAACCCCTGA GCAGGCCAAG GCCTTGGCGA ACACTCTCAC CGCTGAGCAG 
GTCAGTCTGA GCCCTCAACA GGCTGAGGCC CTGGGTATCA CTCCGACCCC TCAGCCGACC 
ACTCTCACCC CTGAGCAGGC CCAGGCCCTG GGTATCACTC CTACCCCACA GCCGATCACA 
CTAACCCCTG AGCAGGCCCA GGCCCTGGGC ATCACTCCGA CCCCACAGCC GACCACACTA 
ACCCCTGAGC AGACCCAGGC CCTGGGTATC ACTCCTACCC CACAGCCGAT CACACTAACC 
CCTGAGCAGG CCCAGGCCCT GGGCATCACT CCGACCCCTC AGCCGATCAC ACTAACCCCT 
GAGCAGACCC AGGCCCTGGG TATCACTCCT ACCCCACAGC CGATCACACT AACCCCTGAG 
CAGGCCCAGG CCCTGGGCAT CACTCCGACC CCTCAGCCGA TCACACTAAC CCCTGAGCAG 
ACCCAGGCCC TGGGTATCAC TCCTACCCCA CAGCCGATCA CACTAACCCC TGAGCAGGCC 
CAGGCCCTGG GCATCACTCC GACCCCTCAG CCGATCACAC TAACCCCTGA GCAGGCCCAG 
GCCCTGGGCA TCACTCCGAC CCCTCAGCCG ATCACACTAA CCCCTGAGCA GGCCCAGGCC 
CTGGGCATCA CTCCGACCCC TCAGCCGATC ACACTAACCC CTGAGCAGAC CCAGGCCCTG 
GGCATCACTC CGACCCCTCA GCCGATCACA CTAACCCCTG AGCAGGCCCA GGCCCTGGGC 
ATCACTCCGA CCCCTCAGCC GATCACACTA ACCCCTGAGC AGGTCCAGGC CCTGGGCATC 
ACTCCGACCC CTCAGCCGAT CACACTAACC CCTGAGCAGG CCCAGGCCCT GGGTATCACT 
CCTACCCCAC AGCCGATCAC ACTAACCCCT GAGCAGGCCC AGGCCCTGGG CATCACTCCG 
ACCCCTCAGC CGATCACACT AACCCCTGAG CAGACCCAGG CCCTGGGTAT CACTCCTACC 
CCACAGCCGA TCACACTAAC CCCTGAGCAG GCCCAGGCCC TGGGCATCAC TCCTACCCCT 
CAGCCGATCA CACTAACCCC TGAGCTGGTC CAGGCCCTGG GCATCACTCC GACCCCTCAG 
CCGATCACAC TAACCCCTGA GCAGGCCCAG GCCCTGGGCA TCACTCCGAC CCCTCAGCCG 
ACCACTCTCA GCCCTGAACA GGCCCAGGCC CTGGGTATCA CTCCGACCCC TCAGCCGATC 
ACACTAACCC CTGAGCAGGC CCAGGCCCTG GGCATCACTC CGACCCCTCA GCCGACCACT 
CTCAGCCCTG AACAGGCCCA GGCTCTGGGG ATTTCTCTCA TTCCTAAACA ACAGGAGATC 
AGTCTTAGTC CAGAGCAGGC CCAAGCTCTG GGGCTCACTC TCACCCCTCA GCAGGCCCAG 
GTACAAAAGA TATATCTCAC TCCTCAGCAG GCCCAGGCTC TGGGAATCAC TGTCAGCCCT 
GAGCAGGCCA AGGCCAAGGG GATCAGTCTG ACCCCTGAGG AGGCCCACTC CCTAGGGATA 
ATTCTCACAG TTGAGCAGGC CAAGGCAAAG AGGATTAATT TGACCCCTCA ACAAGCTCAG 
GACCTGGGGC TCACTCTCAC CCCTGAGCAG GCCCAGGATC TGGGGATTTC TCTCATTCCC 
AAACAAGAGA TCAGTTTTAG TCCCCTGCAG GCCCAAGCAA TGGGGCTCAC TCTCACCCCT 
CAGCAGGCCC AGGTACAAAA GATATATCTC ACTCCTCAGC AGGCCCAGGC TCTGGGAATC 
ACTGTCAGCC CTGAGCAGGC AAAGGGGATC AGTCTGACCC CTGAGGAGGC CCACTCCCTA 
GGGATAATTC TCACAGTTGA GCAGGCCAAG GCACAGAGGA TTAATTTGAC CCCTCAACAA 
GCTCAGGACC TGGGGCTCAC TCTCACCCCT GAGCAGGCCC AGGATCTGGG GATTTCTCTC 
ATTCCCAAAC AACAAGAGAT CAGTTTTAGT CCCCTGCAGG CCCAAGCAAT GGGGCTCACT 
CTCACCCCTC AGCAGGCCCA GGTACAAAAG ATATATCTCA CTCCTCAGCA GGCCCAGGCT 
CTGGGAATCA CTGTCAGCCC TGAGCAGGCA AAGGGGATCA GTCTGACCCC TGAGGAGGCC 
CACTCCCTAG GGATAATTCT CACAGTTGAG CAGGCCAAGG CACAGAGGAT TAATTTGACC 
CCTCAACAAG CTCAGGACCT GGGGATCACT CTCACCCCTG AGCAGGCCCA GGATCTGGGG 
ATTTCTCTCA TTCCCAAACA ACAAGAGATC AGTTTTAGTC CCCTGCAGGC CCAAGCAATG 
GGGCTCACTC TCACCCCTCA GCAGGCCCAG GTACAAAAGA TATATCTCAC TCCTCAGCAG 
GCCCAGGCTC TGGGAATCAC TGTCAGCCCT GAGCAGGCAA AGGGGATCAG TCTGACCCCT 
GAGGAGGCCC ACTCCCTGGG AATCGCTCTC ACAGTTGAGC AGGCCAAGGC ACAGAGGATT 
AATCTGACCC CTCAGCAAGC TCAGGACCTG GGGCTCACTC TCACCCCTGA GCAGGCCCAG 
GATCTGGGAA TAGCTTCTAC CCTTAAGCAG GCTGAAGCTG TGGGAATCAT TCCCACCCCT 
ACCCCTGAGC CTTACCAGGA AAGACGTTTA AGTCTGACCT CTGAGCAGGT TCAGGCCTTG 
AGGACCAGTT TCCCCACTAA GGAGACCCTG TCATTGGGGA TTTATCTCAC CCCTAAGCAG 
GCCCAGTACC TGGGAATCAC TCTCACTGCT AGTCAAGCCA AGGTAATGAA GATTTGCCTC 
ACCCCTGAGC AGGCCCAGGC CCTGGGGATC ACTGTCACCC CTAAGCAGGC CAAGGCACGG 
AGGACCAGTC TGACCCCTGA GCAGGCCCAG GCTCTGGGAG TCATTCTTAC TCCTGAACAG 
GCCCAGGCCC ACAGAATCAC AGTCACTCCT GAGCAGGCCC AGGCTCTGGG GATTGCTCTC 
ACACCTGAGC AGGCCCATGC CCTGGGGATT GCTCTCACAC CTGAGCAGGC CCAGGCACAA 
GGTATTTTTT CTCCTAAGAA ATTCCAGGAA TTGGATGTTC CATTAACCTA TAGACAAACA 
AAAGCACTAA GGAGCCATCT TTCTAAGAAG CAACTTGAAG CCCAGAGAAG TCAAATCCTT 
ATCTCTGAGT CTATCCAAAT CCCCAAACCA GCTGTCGCCT CTAAATATAC CCAGACAATT 
GAAATCCCAC TAACTACCCA ACCATTCCAG GCATTTGGAG CTCTACTTTC AAAGACAAAG 
GAGACAGAAA TGGGAATCTC TCCCCTCACT CCCAAGCAGC CCCAGGCAAT TGTAACTCCA 
CCTTCAAAGA GAAAGGAGAA AGAATTGGGA ATCTCTCCCC TCACTCCCAA GCAGCCCCAG 
GCAGTTGAGC CTGCCAAGGC AAAGCTGCCA CCCCTCACAC CCAGTCAGGC CCAGCCTTTG 
CAGAAGCAGC TTGCTCCAGA GCTCACACAA ACACTGCTGT TCACCATCAC TCTCCAGAAA 
GCCCAGCACT TGGGTGTCAC TTTTACCTAC GAACAAACTC AGGCAGCAGC TGTCACCCTC 
ACCTCTGAGC AGGTGGCAGC ACTAGAAGAC GCTCTCACTG AGAATCTGGC TTGGAGATGG 
GAGATTTCAG TCACACCAGG AATGGCTCAA GAGGCACCAA ACATCACTAC CACTAAGCAA 
CTGCAAGCCC TGGGGATTAC TGCTCGCCAG CCAGCTCAGG CATTTCCCAG CCCTTTTACC 
TTGGAGAAGC CTGCCACGTT GGCTACTTCC ACCGACAGAC TATCTCAAAG ATGGAAGGAT 
TCTTACCCTG CTTCTATTCC ACTGCAGGCA TTGAGGCCTT CTCCTACTCA AGCCCCATTT 
ACCCCTACGA CAAGCCTAGG AATAGGTAGT CTTCTGGACT CTGAGAAGCC ATGGATGTCT 
CCTACCTACA GACAGACTCT CACAGACAGG GGCCAAGACG TTCTTGCACA GCCCCTAGCA 
CCAGAAACCC CTCCTTCCCT CAGACAGCTC CTAGCACCTG GGGCTCCTCC CACTCCAGGG 
CCACCCCTTG GTCCTAGGCA TTTCTTCAAA CCTAGAGTCC CACCCACCTC TGGAGAGGTG 
CCAGGACTTG TTTCTGGAGG CTCTGCTGCC CATGAGGAGC TCCCAATGTC CAGAACCACC 
CCCCTCCAGC CTCCTGAATG GCAAGGCCCA TCAAGGCTCA TTCCCGAGCA AGGCTTCATG 
CCAGCAATCT CTTCCATCCC ACTGCATCCT TTCACAGCAG AGGCCCTACC AACCCCTGGA 
AGACCTCAGA GAAGTTCAAA AGCCAAACCT CTAAAACCTA AATCAGCAAG AGGTCTCCCA 
AATGTCACTC TGGGTTTTGA GACCTCTCAG GCTCCATTCC CTATTGAGAA GACCCAAATA 
CCTAAGACTC CTGACACTTC AGAACAAACT CAAGCACTCC AAGATGCCTT GGGTGTGCAA 
CCATTTGGAA TATTTCAGCC TTATGGCACC AGCTCCGGGA TAGCTCGGTC ACAGTCCCCT 
CTCATTGATG AGAAAGCCCT TTCTAGAGAG AAGCCTGGAA CACCACTACC ATCTCTTACC 
ACTCAACTCC CCCAAACACC ACAGATCTCA ACTTCTGAAA AGGGCCAGAA ACCATGGCTC 
CCGCCGATAG ACAAGCCCTG GACACCAACC CCTGTTTCTA GTACCAGAGA AGCCAAGATG 
ATAGTGTCCC CCACTGATCA GCACCCTGAA GACGGCTATG TCGTCGATGT GGAGGCTCAG 
AGGAAGAACC TGGTCACATT AAATCAGGCG GCACAAACTT CTGCACTCCC TGCACAGTAT 
CTCACAATTG CCAAGAATCT CATAATCGAA TTACTTCATA TAGATACAGT TCGGCTGGGG 
TATCTGTCCC GAAAGTATGT TGCCTATAGG CTGATCCAGC TTGCCAGAAA CCACCTAACC 
AAACGAGTGA AAACCATCCA AAACACTGGG AAAGGGTACG AGGTTCAGAG CTTGTACACG 
ATGCTTGATA GGATTGACCA GTACCAGAAG AAGGTGATGC ACAGCTGGAC GGACAAGCAG 
AAGCAGCTGG AACAGAGACG AAAGCAGTGC CTACGGTCTA TGACGCAGTT CTTCAGCCAG 
CTTGAAAGGG GATATAAACT CAGTCTTAGC CAGCCCATGC CTTCGATCCC CAGCTTTAAG 
AAGATACCCG GATTTACCAA ACTTCAGCGG CCGGTCTTGG AGTTATTAAT TGATGACAGC 
AAAAGATCTG ACCTCTTCAA AACACTCGGA CAAGCTTCAG TGGAGGCCGT GTGGAACGCT 
GACCTGTCTA CGTCAAGCTA CCCAATAATA GAGAAGGCAC CGATGAGTGC GCTCTGGGCC 
CAGCTGGGTG GGTACCCAGA TATTCCCAAG CTGCTGCAGT TAGACATTCA GTCGACCTTC 
AGAAAATCTC TTGCTTCCAT CCGGTCACAG TCTAAGAAGA TTCGTAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam186a ( XM_030248786.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104646.1 Mus musculus family with sequence similarity 186, member A (Fam186a), transcript variant X2, mRNA.

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CloneID	OMu51616
Clone ID Related Accession (Same CDS sequence)	XM_030248786.1
Accession Version	XM_030248786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	protein FAM186A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTCA AAAGGCAGAG CAAAGTCTAC TATGAATCTG ACTCAGATGA TGATGATGAT 
GAATTCAAGG AACTTGCCAC GTTGAGAAAG CCCATAAGCA ACCGTGATAA TCCACTGAGC 
TTGCAATTTG AGATCCCAGC ATCAGTACAG TCAGTCATCC ATAAGATTGA GGAATCACAT 
ATCTTTCGGG CCAAAGAGGA AGTTATTTGG AGGCTGACTG AAATAATGAG CAACGTGGAG 
CTGATAATGA CTCGGTATAA TATCGACAGC ATGTCCCCTG GGAGGAAGGG TTCCAGCTCA 
GAAAGCCAGA AAAAGAAGAG AAAGGCTTTT TTAGAAAAGA TTGCCACAGT TATGACGAAT 
GTTGACCTTA GAGAAAGGAC TCTCTCCAAG ATTCTGTCCT GGCTAGAAGA ATGGAATCTT 
ATTCTGTCCG AGGTGTCTGC CATCAACATG GATGACTACT ACCACTGGAC TGTGAAGATG 
GAGCTGATAC CAGACACGCT GAAAAGGATC AGCAAGAATG TCGACTCTCT GATTCAAATG 
GCCTTATTAC TCGTGGAAGA GAAGAAGAGA GCGAAGAAAA GAATATTGGC TAGAGGCACT 
CTCTGGAAAG CTTGGAAAGA CCGAGCTATA AAGCGGCCTG CCACAGCCCA AGCTTTAAGA 
CTAGATCAGA TGATTTTTGA CCAGATCGGC TTAAACGCTA AGGTTTCAGA GATTCAGGGC 
ATGCTGCAGG AGCTCATCGG CACAGCGATG TTCAGCAAGC TGGAAAATAC TGCCATTAAA 
TACATGTCCA CCACAGTCAT CAATCTCTCC AAGGCCCTGA ACACCGTCAG CGATGAACTA 
AAACTCGTGA GCTCTCGAGT GGTCAAGTCT GTTCTGAAGG AAGAGCAAGA GGAAGATAAG 
GAGAAAGACC GCTCCAAGCA GGTCATCCAA GAACTCAGCG AGGAGAACGA GATGCTCCAG 
CAGAAACTTA GAGAATCCGA AGAAAAATGC GACCAACTTA TTCGCATCAA AAATTATCTT 
GGACGCCAGA TGATACACCC GGCTGGGTCC TTGAAAATAC TGCCTTTACA GGGGCCGTTT 
TTCAGCAGGG AGTCCAGGGA CTCTGAGAGG GAAGGAAAGA CAGACGACTC CCAGAAGGAA 
GGGACCAAAA GCTCCACAGT GACATGGGAC CCATCTTCCA GTGAAGGGGC TCCGGATGCA 
GCTAAGCAGC AAGTTGAGGA CAAAGCTGAG GCCCCCCAAG ACCAGATAAA ACTCAAAGGC 
CTGCCACTGA CAGAGGAGAC CTTGGACACA AATCTGGAAA CGGAGGAAAA GAAACTCACT 
CAACTGGATA TTCTAAAAGC AATGGACAAG AAGAAAGTGG GTAAAGCAAA GGCCGAGCAA 
GGCAGAGGGG GACCAAGTCC CATGTGGGAA CAACTCAAGA AGGGCAGATC TGAGCAGCTG 
ATTCCAGGCA GCACAGGCTC ACCAGAGAGT AGAGAGTCCA CCCTTAAATT AACAGACAAA 
GAAGCCAAGA GTGAAATAGA TCTGCTTCAG CCTGAGGGTA CCCCAGAGCT ACAAAAACCA 
GAAACAAAGT GGAAAAGACT CATAGCAAAA ACTGAAGAAA CAAAACGGTC TGGTAAGTTA 
CAACCCAAGA CTTCTAAGGT GACTTCAGTA ACCCCTGATG AAGGTGAGCA GGGCAGTCTA 
GAATCATTTC AAAGAGCCAT CCTGGCCTTC CTGAGAGAGA AGACGAATAA TGTAGGAAAG 
GCTTTTGATC CAAAGAGTAT ACGGAAGGAG GAGTCGTCAC TGGGAAAAGC AGAAGTTGAA 
AATTTAAACG TCATAAAGGC AAAAATAGAG GAATATTTCC AAAAAGTGGC TGAAACTGTG 
ACTGTAGCCT TGAGGACATA CAGAGATGAA AGAAAGGTTC AACCTAAAGA GAAGCCCAAG 
AAACAGCCAA AGGGAGCACT GTCACGGATG CTTCCTAAGC AGGGGGCGGT CGGCGCCAAG 
TCTGAGGTTG GCAGAGTTCT CCTCAGTGAG ATCACAGACC CAGCAATTAA GAAACTGGTA 
CAGATGCTCT TAAATGAGAT AGAGAGTGAC TTCAAATACA AAACGACGAA GGAACAGCCA 
CAGGCGGCGG TGGCGGCAGC GGCAGCGGCA GCGGCTGTGC AGAAGGAAGA GCCAGGACTG 
AGGACGAAGG ACAAAACGGA GCTGCTGGAG GTGTGGATGC AAGCATTCAA AGACTCACAA 
GGCCACCAGG TTCAGGACCT GGGAGATGCA GAGGATGCAG AAAAGCCGAA GTGGAGCGAG 
CCGGGTCATC AGGACCTGTG GAAGGGTGTC AGCAAGACCT CCATCAATCT AACTCCCAGG 
CTGATGAAGG CCCCGAAACC GCAGATAAGT CAGGCGCAGG CGCTCCAGGT GGAGCCTGGT 
GTAATGGGGG CCACGGAGGA ACAGCAGCCC AAACTCACTC TCTCCTCTTC CAGAATCCCC 
TTCGTTTCTC CAAAGCCCCA CCAAGTGCAT GTCCCTGAGC AGGCCCAGAT AATAGGCCCT 
CAGATGTCCC CTCAGCTTTC AATGGCATCT CTCACCTCTG AGCAGGCACA AACTTTGAGT 
GGTCCTCTTA CTCATGAACA CACTATAGAA TTAAAGACTG CTTTCACAGC AGGGCAGGAG 
CAAGAGCTTG GGGCTAAGCT GATGGTTCCT AGGATTCTTA AGCCGACACA GACTCCAAAG 
GAGTCTCTCA TATCAGAAAA ACCTGAGGCA GTGCAGATCT CCCAAGTGAC TACTGAAGAG 
ATATCTGGAG TCACAGATAC CCAAAGAACA ATTCCACCCT TCCAGCAGAT CCTGGAACAA 
GAAACCACTC CCACTCCTGA GCAAGACAAG GAACCAAGGA TTACTCTCAC CCGTCAGCAG 
ACTGAGGCCC TAGGTATCAC TCTCACCCCT GAGCAAACTA AGTCCCAGAG GATTAGTCTA 
ACCCATGAGC AGGCTCGGGC CCTAGGGGTC ACTCTCACTC CTGAGCAGTA CAAAGAGGAC 
AGAATCAGTC TGACTCCCCT ACAGGCCCAG GTCCTGGGAA TCACTCTCAA CCTTCAGCAG 
GCTAAGGCCC TGGGGATCAC TCTCACTCCT GAGCAAGTCA AGGCACAGAG GGTCAATCTG 
ATCCCTCAAC AGTACCAGGT CCACGGAACC ACTCTCACCA CTCAGCAGGC TGAAGCACAA 
AGAACTAATC TCACGCCCGA GCAAGCCAAG GCCCTGGGTC TCCCTCTGAT CCCTCCTAAG 
CCAATCACTT TCACCCGTGA GCAGACCCAG GCCCTGGGTA TCACTCCAAC CCATCAGCCA 
ATCACTCTCA CCTCTGAGCA GGTCCAGGCC CTGGGTATCA CTCCAACCCA TCAGCCAATC 
ACTCTCACCC CTGAGCAGGC CCAGGCCCTG GCACTCATTC TCACCACTGA GCAGGTCAAG 
ACTCAGAGAA TCAATCTGAG CCCTGATCAG ACCCAGGCCC TGGGTATCAC CCCGACCCCT 
CAGCCGATCA CTTTCACCCC TGAGCAGACC CAGGCTCTGG GTATCACCCC AACCCCTCAG 
CTGATCACAC TAACCCCTGA GCAGGCCAAG GCCTTGGCGA ACACTCTCAC CGCTGAGCAG 
GTCAGTCTGA GCCCTCAACA GGCTGAGGCC CTGGGTATCA CTCCGACCCC TCAGCCGACC 
ACTCTCACCC CTGAGCAGGC CCAGGCCCTG GGTATCACTC CTACCCCACA GCCGATCACA 
CTAACCCCTG AGCAGGCCCA GGCCCTGGGC ATCACTCCGA CCCCTCAGCC GATCACACTA 
ACCCCTGAGC AGACCCAGGC CCTGGGTATC ACTCCTACCC CACAGCCGAT CACACTAACC 
CCTGAGCAGG CCCAGGCCCT GGGCATCACT CCGACCCCTC AGCCGATCAC ACTAACCCCT 
GAGCAGACCC AGGCCCTGGG TATCACTCCT ACCCCACAGC CGATCACACT AACCCCTGAG 
CAGGCCCAGG CCCTGGGCAT CACTCCGACC CCTCAGCCGA TCACACTAAC CCCTGAGCAG 
GCCCAGGCCC TGGGCATCAC TCCGACCCCT CAGCCGATCA CACTAACCCC TGAGCAGGCC 
CAGGCCCTGG GCATCACTCC GACCCCTCAG CCGATCACAC TAACCCCTGA GCAGACCCAG 
GCCCTGGGCA TCACTCCGAC CCCTCAGCCG ATCACACTAA CCCCTGAGCA GGCCCAGGCC 
CTGGGCATCA CTCCGACCCC TCAGCCGATC ACACTAACCC CTGAGCAGGT CCAGGCCCTG 
GGCATCACTC CGACCCCTCA GCCGATCACA CTAACCCCTG AGCAGGCCCA GGCCCTGGGT 
ATCACTCCTA CCCCACAGCC GATCACACTA ACCCCTGAGC AGGCCCAGGC CCTGGGCATC 
ACTCCGACCC CTCAGCCGAT CACACTAACC CCTGAGCAGA CCCAGGCCCT GGGTATCACT 
CCTACCCCAC AGCCGATCAC ACTAACCCCT GAGCAGGCCC AGGCCCTGGG CATCACTCCT 
ACCCCTCAGC CGATCACACT AACCCCTGAG CTGGTCCAGG CCCTGGGCAT CACTCCGACC 
CCTCAGCCGA TCACACTAAC CCCTGAGCAG GCCCAGGCCC TGGGCATCAC TCCGACCCCT 
CAGCCGACCA CTCTCAGCCC TGAACAGGCC CAGGCCCTGG GTATCACTCC GACCCCTCAG 
CCGATCACAC TAACCCCTGA GCAGGCCCAG GCCCTGGGCA TCACTCCGAC CCCTCAGCCG 
ACCACTCTCA GCCCTGAACA GGCCCAGGCT CTGGGGATTT CTCTCATTCC TAAACAACAG 
GAGATCAGTC TTAGTCCAGA GCAGGCCCAA GCTCTGGGGC TCACTCTCAC CCCTCAGCAG 
GCCCAGGTAC AAAAGATATA TCTCACTCCT CAGCAGGCCC AGGCTCTGGG AATCACTGTC 
AGCCCTGAGC AGGCCAAGGC CAAGGGGATC AGTCTGACCC CTGAGGAGGC CCACTCCCTA 
GGGATAATTC TCACAGTTGA GCAGGCCAAG GCAAAGAGGA TTAATTTGAC CCCTCAACAA 
GCTCAGGACC TGGGGCTCAC TCTCACCCCT GAGCAGGCCC AGGATCTGGG GATTTCTCTC 
ATTCCCAAAC AAGAGATCAG TTTTAGTCCC CTGCAGGCCC AAGCAATGGG GCTCACTCTC 
ACCCCTCAGC AGGCCCAGGT ACAAAAGATA TATCTCACTC CTCAGCAGGC CCAGGCTCTG 
GGAATCACTG TCAGCCCTGA GCAGGCAAAG GGGATCAGTC TGACCCCTGA GGAGGCCCAC 
TCCCTAGGGA TAATTCTCAC AGTTGAGCAG GCCAAGGCAC AGAGGATTAA TTTGACCCCT 
CAACAAGCTC AGGACCTGGG GCTCACTCTC ACCCCTGAGC AGGCCCAGGA TCTGGGGATT 
TCTCTCATTC CCAAACAACA AGAGATCAGT TTTAGTCCCC TGCAGGCCCA AGCAATGGGG 
CTCACTCTCA CCCCTCAGCA GGCCCAGGTA CAAAAGATAT ATCTCACTCC TCAGCAGGCC 
CAGGCTCTGG GAATCACTGT CAGCCCTGAG CAGGCAAAGG GGATCAGTCT GACCCCTGAG 
GAGGCCCACT CCCTAGGGAT AATTCTCACA GTTGAGCAGG CCAAGGCACA GAGGATTAAT 
TTGACCCCTC AACAAGCTCA GGACCTGGGG ATCACTCTCA CCCCTGAGCA GGCCCAGGAT 
CTGGGGATTT CTCTCATTCC CAAACAACAA GAGATCAGTT TTAGTCCCCT GCAGGCCCAA 
GCAATGGGGC TCACTCTCAC CCCTCAGCAG GCCCAGGTAC AAAAGATATA TCTCACTCCT 
CAGCAGGCCC AGGCTCTGGG AATCACTGTC AGCCCTGAGC AGGCAAAGGG GATCAGTCTG 
ACCCCTGAGG AGGCCCACTC CCTGGGAATC GCTCTCACAG TTGAGCAGGC CAAGGCACAG 
AGGATTAATC TGACCCCTCA GCAAGCTCAG GACCTGGGGC TCACTCTCAC CCCTGAGCAG 
GCCCAGGATC TGGGAATAGC TTCTACCCTT AAGCAGGCTG AAGCTGTGGG AATCATTCCC 
ACCCCTACCC CTGAGCCTTA CCAGGAAAGA CGTTTAAGTC TGACCTCTGA GCAGGTTCAG 
GCCTTGAGGA CCAGTTTCCC CACTAAGGAG ACCCTGTCAT TGGGGATTTA TCTCACCCCT 
AAGCAGGCCC AGTACCTGGG AATCACTCTC ACTGCTAGTC AAGCCAAGGT AATGAAGATT 
TGCCTCACCC CTGAGCAGGC CCAGGCCCTG GGGATCACTG TCACCCCTAA GCAGGCCAAG 
GCACGGAGGA CCAGTCTGAC CCCTGAGCAG GCCCAGGCTC TGGGAGTCAT TCTTACTCCT 
GAACAGGCCC AGGCCCACAG AATCACAGTC ACTCCTGAGC AGGCCCAGGC TCTGGGGATT 
GCTCTCACAC CTGAGCAGGC CCATGCCCTG GGGATTGCTC TCACACCTGA GCAGGCCCAG 
GCACAAGGTA TTTTTTCTCC TAAGAAATTC CAGGAATTGG ATGTTCCATT AACCTATAGA 
CAAACAAAAG CACTAAGGAG CCATCTTTCT AAGAAGCAAC TTGAAGCCCA GAGAAGTCAA 
ATCCTTATCT CTGAGTCTAT CCAAATCCCC AAACCAGCTG TCGCCTCTAA ATATACCCAG 
ACAATTGAAA TCCCACTAAC TACCCAACCA TTCCAGGCAT TTGGAGCTCT ACTTTCAAAG 
ACAAAGGAGA CAGAAATGGG AATCTCTCCC CTCACTCCCA AGCAGCCCCA GGCAATTGTA 
ACTCCACCTT CAAAGAGAAA GGAGAAAGAA TTGGGAATCT CTCCCCTCAC TCCCAAGCAG 
CCCCAGGCAG TTGAGCCTGC CAAGGCAAAG CTGCCACCCC TCACACCCAG TCAGGCCCAG 
CCTTTGCAGA AGCAGCTTGC TCCAGAGCTC ACACAAACAC TGCTGTTCAC CATCACTCTC 
CAGAAAGCCC AGCACTTGGG TGTCACTTTT ACCTACGAAC AAACTCAGGC AGCAGCTGTC 
ACCCTCACCT CTGAGCAGGT GGCAGCACTA GAAGACGCTC TCACTGAGAA TCTGGCTTGG 
AGATGGGAGA TTTCAGTCAC ACCAGGAATG GCTCAAGAGG CACCAAACAT CACTACCACT 
AAGCAACTGC AAGCCCTGGG GATTACTGCT CGCCAGCCAG CTCAGGCATT TCCCAGCCCT 
TTTACCTTGG AGAAGCCTGC CACGTTGGCT ACTTCCACCG ACAGACTATC TCAAAGATGG 
AAGGATTCTT ACCCTGCTTC TATTCCACTG CAGGCATTGA GGCCTTCTCC TACTCAAGCC 
CCATTTACCC CTACGACAAG CCTAGGAATA GGTAGTCTTC TGGACTCTGA GAAGCCATGG 
ATGTCTCCTA CCTACAGACA GACTCTCACA GACAGGGGCC AAGACGTTCT TGCACAGCCC 
CTAGCACCAG AAACCCCTCC TTCCCTCAGA CAGCTCCTAG CACCTGGGGC TCCTCCCACT 
CCAGGGCCAC CCCTTGGTCC TAGGCATTTC TTCAAACCTA GAGTCCCACC CACCTCTGGA 
GAGGTGCCAG GACTTGTTTC TGGAGGCTCT GCTGCCCATG AGGAGCTCCC AATGTCCAGA 
ACCACCCCCC TCCAGCCTCC TGAATGGCAA GGCCCATCAA GGCTCATTCC CGAGCAAGGC 
TTCATGCCAG CAATCTCTTC CATCCCACTG CATCCTTTCA CAGCAGAGGC CCTACCAACC 
CCTGGAAGAC CTCAGAGAAG TTCAAAAGCC AAACCTCTAA AACCTAAATC AGCAAGAGGT 
CTCCCAAATG TCACTCTGGG TTTTGAGACC TCTCAGGCTC CATTCCCTAT TGAGAAGACC 
CAAATACCTA AGACTCCTGA CACTTCAGAA CAAACTCAAG CACTCCAAGA TGCCTTGGGT 
GTGCAACCAT TTGGAATATT TCAGCCTTAT GGCACCAGCT CCGGGATAGC TCGGTCACAG 
TCCCCTCTCA TTGATGAGAA AGCCCTTTCT AGAGAGAAGC CTGGAACACC ACTACCATCT 
CTTACCACTC AACTCCCCCA AACACCACAG ATCTCAACTT CTGAAAAGGG CCAGAAACCA 
TGGCTCCCGC CGATAGACAA GCCCTGGACA CCAACCCCTG TTTCTAGTAC CAGAGAAGCC 
AAGATGATAG TGTCCCCCAC TGATCAGCAC CCTGAAGACG GCTATGTCGT CGATGTGGAG 
GCTCAGAGGA AGAACCTGGT CACATTAAAT CAGGCGGCAC AAACTTCTGC ACTCCCTGCA 
CAGTATCTCA CAATTGCCAA GAATCTCATA ATCGAATTAC TTCATATAGA TACAGTTCGG 
CTGGGGTATC TGTCCCGAAA GTATGTTGCC TATAGGCTGA TCCAGCTTGC CAGAAACCAC 
CTAACCAAAC GAGTGAAAAC CATCCAAAAC ACTGGGAAAG GGTACGAGGT TCAGAGCTTG 
TACACGATGC TTGATAGGAT TGACCAGTAC CAGAAGAAGG TGATGCACAG CTGGACGGAC 
AAGCAGAAGC AGCTGGAACA GAGACGAAAG CAGTGCCTAC GGTCTATGAC GCAGTTCTTC 
AGCCAGCTTG AAAGGGGATA TAAACTCAGT CTTAGCCAGC CCATGCCTTC GATCCCCAGC 
TTTAAGAAGA TACCCGGATT TACCAAACTT CAGCGGCCGG TCTTGGAGTT ATTAATTGAT 
GACAGCAAAA GATCTGACCT CTTCAAAACA CTCGGACAAG CTTCAGTGGA GGCCGTGTGG 
AACGCTGACC TGTCTACGTC AAGCTACCCA ATAATAGAGA AGGCACCGAT GAGTGCGCTC 
TGGGCCCAGC TGGGTGGGTA CCCAGATATT CCCAAGCTGC TGCAGTTAGA CATTCAGTCG 
ACCTTCAGAA AATCTCTTGC TTCCATCCGG TCACAGTCTA AGAAGATTCG TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104646.1
CDS	147..9083
Translation	MPVKRQSKVYYESDSDDDDDEFKELATLRKPISNRDNPLSLQFEIPASVQSVIHKIEESHIFRAKEEVIWRLTEIMSNVELIMTRYNIDSMSPGRKGSSSESQKKKRKAFLEKIATVMTNVDLRERTLSKILSWLEEWNLILSEVSAINMDDYYHWTVKMELIPDTLKRISKNVDSLIQMALLLVEEKKRAKKRILARGTLWKAWKDRAIKRPATAQALRLDQMIFDQIGLNAKVSEIQGMLQELIGTAMFSKLENTAIKYMSTTVINLSKALNTVSDELKLVSSRVVKSVLKEEQEEDKEKDRSKQVIQELSEENEMLQQKLRESEEKCDQLIRIKNYLGRQMIHPAGSLKILPLQGPFFSRESRDSEREGKTDDSQKEGTKSSTVTWDPSSSEGAPDAAKQQVEDKAEAPQDQIKLKGLPLTEETLDTNLETEEKKLTQLDILKAMDKKKVGKAKAEQGRGGPSPMWEQLKKGRSEQLIPGSTGSPESRESTLKLTDKEAKSEIDLLQPEGTPELQKPETKWKRLIAKTEETKRSGKLQPKTSKVTSVTPDEGEQGSLESFQRAILAFLREKTNNVGKAFDPKSIRKEESSLGKAEVENLNVIKAKIEEYFQKVAETVTVALRTYRDERKVQPKEKPKKQPKGALSRMLPKQGAVGAKSEVGRVLLSEITDPAIKKLVQMLLNEIESDFKYKTTKEQPQAAVAAAAAAAAVQKEEPGLRTKDKTELLEVWMQAFKDSQGHQVQDLGDAEDAEKPKWSEPGHQDLWKGVSKTSINLTPRLMKAPKPQISQAQALQVEPGVMGATEEQQPKLTLSSSRIPFVSPKPHQVHVPEQAQIIGPQMSPQLSMASLTSEQAQTLSGPLTHEHTIELKTAFTAGQEQELGAKLMVPRILKPTQTPKESLISEKPEAVQISQVTTEEISGVTDTQRTIPPFQQILEQETTPTPEQDKEPRITLTRQQTEALGITLTPEQTKSQRISLTHEQARALGVTLTPEQYKEDRISLTPLQAQVLGITLNLQQAKALGITLTPEQVKAQRVNLIPQQYQVHGTTLTTQQAEAQRTNLTPEQAKALGLPLIPPKPITFTREQTQALGITPTHQPITLTSEQVQALGITPTHQPITLTPEQAQALALILTTEQVKTQRINLSPDQTQALGITPTPQPITFTPEQTQALGITPTPQLITLTPEQAKALANTLTAEQVSLSPQQAEALGITPTPQPTTLTPEQAQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQTQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQTQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQTQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQVQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQTQALGITPTPQPITLTPEQAQALGITPTPQPITLTPELVQALGITPTPQPITLTPEQAQALGITPTPQPTTLSPEQAQALGITPTPQPITLTPEQAQALGITPTPQPTTLSPEQAQALGISLIPKQQEISLSPEQAQALGLTLTPQQAQVQKIYLTPQQAQALGITVSPEQAKAKGISLTPEEAHSLGIILTVEQAKAKRINLTPQQAQDLGLTLTPEQAQDLGISLIPKQEISFSPLQAQAMGLTLTPQQAQVQKIYLTPQQAQALGITVSPEQAKGISLTPEEAHSLGIILTVEQAKAQRINLTPQQAQDLGLTLTPEQAQDLGISLIPKQQEISFSPLQAQAMGLTLTPQQAQVQKIYLTPQQAQALGITVSPEQAKGISLTPEEAHSLGIILTVEQAKAQRINLTPQQAQDLGITLTPEQAQDLGISLIPKQQEISFSPLQAQAMGLTLTPQQAQVQKIYLTPQQAQALGITVSPEQAKGISLTPEEAHSLGIALTVEQAKAQRINLTPQQAQDLGLTLTPEQAQDLGIASTLKQAEAVGIIPTPTPEPYQERRLSLTSEQVQALRTSFPTKETLSLGIYLTPKQAQYLGITLTASQAKVMKICLTPEQAQALGITVTPKQAKARRTSLTPEQAQALGVILTPEQAQAHRITVTPEQAQALGIALTPEQAHALGIALTPEQAQAQGIFSPKKFQELDVPLTYRQTKALRSHLSKKQLEAQRSQILISESIQIPKPAVASKYTQTIEIPLTTQPFQAFGALLSKTKETEMGISPLTPKQPQAIVTPPSKRKEKELGISPLTPKQPQAVEPAKAKLPPLTPSQAQPLQKQLAPELTQTLLFTITLQKAQHLGVTFTYEQTQAAAVTLTSEQVAALEDALTENLAWRWEISVTPGMAQEAPNITTTKQLQALGITARQPAQAFPSPFTLEKPATLATSTDRLSQRWKDSYPASIPLQALRPSPTQAPFTPTTSLGIGSLLDSEKPWMSPTYRQTLTDRGQDVLAQPLAPETPPSLRQLLAPGAPPTPGPPLGPRHFFKPRVPPTSGEVPGLVSGGSAAHEELPMSRTTPLQPPEWQGPSRLIPEQGFMPAISSIPLHPFTAEALPTPGRPQRSSKAKPLKPKSARGLPNVTLGFETSQAPFPIEKTQIPKTPDTSEQTQALQDALGVQPFGIFQPYGTSSGIARSQSPLIDEKALSREKPGTPLPSLTTQLPQTPQISTSEKGQKPWLPPIDKPWTPTPVSSTREAKMIVSPTDQHPEDGYVVDVEAQRKNLVTLNQAAQTSALPAQYLTIAKNLIIELLHIDTVRLGYLSRKYVAYRLIQLARNHLTKRVKTIQNTGKGYEVQSLYTMLDRIDQYQKKVMHSWTDKQKQLEQRRKQCLRSMTQFFSQLERGYKLSLSQPMPSIPSFKKIPGFTKLQRPVLELLIDDSKRSDLFKTLGQASVEAVWNADLSTSSYPIIEKAPMSALWAQLGGYPDIPKLLQLDIQSTFRKSLASIRSQSKKIRK

Target ORF information:

RefSeq Version	XM_030248786.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus family with sequence similarity 186, member A (Fam186a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248786.1

ORF Insert Sequence:

ATGCCTGTCA AAAGGCAGAG CAAAGTCTAC TATGAATCTG ACTCAGATGA TGATGATGAT 
GAATTCAAGG AACTTGCCAC GTTGAGAAAG CCCATAAGCA ACCGTGATAA TCCACTGAGC 
TTGCAATTTG AGATCCCAGC ATCAGTACAG TCAGTCATCC ATAAGATTGA GGAATCACAT 
ATCTTTCGGG CCAAAGAGGA AGTTATTTGG AGGCTGACTG AAATAATGAG CAACGTGGAG 
CTGATAATGA CTCGGTATAA TATCGACAGC ATGTCCCCTG GGAGGAAGGG TTCCAGCTCA 
GAAAGCCAGA AAAAGAAGAG AAAGGCTTTT TTAGAAAAGA TTGCCACAGT TATGACGAAT 
GTTGACCTTA GAGAAAGGAC TCTCTCCAAG ATTCTGTCCT GGCTAGAAGA ATGGAATCTT 
ATTCTGTCCG AGGTGTCTGC CATCAACATG GATGACTACT ACCACTGGAC TGTGAAGATG 
GAGCTGATAC CAGACACGCT GAAAAGGATC AGCAAGAATG TCGACTCTCT GATTCAAATG 
GCCTTATTAC TCGTGGAAGA GAAGAAGAGA GCGAAGAAAA GAATATTGGC TAGAGGCACT 
CTCTGGAAAG CTTGGAAAGA CCGAGCTATA AAGCGGCCTG CCACAGCCCA AGCTTTAAGA 
CTAGATCAGA TGATTTTTGA CCAGATCGGC TTAAACGCTA AGGTTTCAGA GATTCAGGGC 
ATGCTGCAGG AGCTCATCGG CACAGCGATG TTCAGCAAGC TGGAAAATAC TGCCATTAAA 
TACATGTCCA CCACAGTCAT CAATCTCTCC AAGGCCCTGA ACACCGTCAG CGATGAACTA 
AAACTCGTGA GCTCTCGAGT GGTCAAGTCT GTTCTGAAGG AAGAGCAAGA GGAAGATAAG 
GAGAAAGACC GCTCCAAGCA GGTCATCCAA GAACTCAGCG AGGAGAACGA GATGCTCCAG 
CAGAAACTTA GAGAATCCGA AGAAAAATGC GACCAACTTA TTCGCATCAA AAATTATCTT 
GGACGCCAGA TGATACACCC GGCTGGGTCC TTGAAAATAC TGCCTTTACA GGGGCCGTTT 
TTCAGCAGGG AGTCCAGGGA CTCTGAGAGG GAAGGAAAGA CAGACGACTC CCAGAAGGAA 
GGGACCAAAA GCTCCACAGT GACATGGGAC CCATCTTCCA GTGAAGGGGC TCCGGATGCA 
GCTAAGCAGC AAGTTGAGGA CAAAGCTGAG GCCCCCCAAG ACCAGATAAA ACTCAAAGGC 
CTGCCACTGA CAGAGGAGAC CTTGGACACA AATCTGGAAA CGGAGGAAAA GAAACTCACT 
CAACTGGATA TTCTAAAAGC AATGGACAAG AAGAAAGTGG GTAAAGCAAA GGCCGAGCAA 
GGCAGAGGGG GACCAAGTCC CATGTGGGAA CAACTCAAGA AGGGCAGATC TGAGCAGCTG 
ATTCCAGGCA GCACAGGCTC ACCAGAGAGT AGAGAGTCCA CCCTTAAATT AACAGACAAA 
GAAGCCAAGA GTGAAATAGA TCTGCTTCAG CCTGAGGGTA CCCCAGAGCT ACAAAAACCA 
GAAACAAAGT GGAAAAGACT CATAGCAAAA ACTGAAGAAA CAAAACGGTC TGGTAAGTTA 
CAACCCAAGA CTTCTAAGGT GACTTCAGTA ACCCCTGATG AAGGTGAGCA GGGCAGTCTA 
GAATCATTTC AAAGAGCCAT CCTGGCCTTC CTGAGAGAGA AGACGAATAA TGTAGGAAAG 
GCTTTTGATC CAAAGAGTAT ACGGAAGGAG GAGTCGTCAC TGGGAAAAGC AGAAGTTGAA 
AATTTAAACG TCATAAAGGC AAAAATAGAG GAATATTTCC AAAAAGTGGC TGAAACTGTG 
ACTGTAGCCT TGAGGACATA CAGAGATGAA AGAAAGGTTC AACCTAAAGA GAAGCCCAAG 
AAACAGCCAA AGGGAGCACT GTCACGGATG CTTCCTAAGC AGGGGGCGGT CGGCGCCAAG 
TCTGAGGTTG GCAGAGTTCT CCTCAGTGAG ATCACAGACC CAGCAATTAA GAAACTGGTA 
CAGATGCTCT TAAATGAGAT AGAGAGTGAC TTCAAATACA AAACGACGAA GGAACAGCCA 
CAGGCGGCGG TGGCGGCAGC GGCAGCGGCA GCGGCTGTGC AGAAGGAAGA GCCAGGACTG 
AGGACGAAGG ACAAAACGGA GCTGCTGGAG GTGTGGATGC AAGCATTCAA AGACTCACAA 
GGCCACCAGG TTCAGGACCT GGGAGATGCA GAGGATGCAG AAAAGCCGAA GTGGAGCGAG 
CCGGGTCATC AGGACCTGTG GAAGGGTGTC AGCAAGACCT CCATCAATCT AACTCCCAGG 
CTGATGAAGG CCCCGAAACC GCAGATAAGT CAGGCGCAGG CGCTCCAGGT GGAGCCTGGT 
GTAATGGGGG CCACGGAGGA ACAGCAGCCC AAACTCACTC TCTCCTCTTC CAGAATCCCC 
TTCGTTTCTC CAAAGCCCCA CCAAGTGCAT GTCCCTGAGC AGGCCCAGAT AATAGGCCCT 
CAGATGTCCC CTCAGCTTTC AATGGCATCT CTCACCTCTG AGCAGGCACA AACTTTGAGT 
GGTCCTCTTA CTCATGAACA CACTATAGAA TTAAAGACTG CTTTCACAGC AGGGCAGGAG 
CAAGAGCTTG GGGCTAAGCT GATGGTTCCT AGGATTCTTA AGCCGACACA GACTCCAAAG 
GAGTCTCTCA TATCAGAAAA ACCTGAGGCA GTGCAGATCT CCCAAGTGAC TACTGAAGAG 
ATATCTGGAG TCACAGATAC CCAAAGAACA ATTCCACCCT TCCAGCAGAT CCTGGAACAA 
GAAACCACTC CCACTCCTGA GCAAGACAAG GAACCAAGGA TTACTCTCAC CCGTCAGCAG 
ACTGAGGCCC TAGGTATCAC TCTCACCCCT GAGCAAACTA AGTCCCAGAG GATTAGTCTA 
ACCCATGAGC AGGCTCGGGC CCTAGGGGTC ACTCTCACTC CTGAGCAGTA CAAAGAGGAC 
AGAATCAGTC TGACTCCCCT ACAGGCCCAG GTCCTGGGAA TCACTCTCAA CCTTCAGCAG 
GCTAAGGCCC TGGGGATCAC TCTCACTCCT GAGCAAGTCA AGGCACAGAG GGTCAATCTG 
ATCCCTCAAC AGTACCAGGT CCACGGAACC ACTCTCACCA CTCAGCAGGC TGAAGCACAA 
AGAACTAATC TCACGCCCGA GCAAGCCAAG GCCCTGGGTC TCCCTCTGAT CCCTCCTAAG 
CCAATCACTT TCACCCGTGA GCAGACCCAG GCCCTGGGTA TCACTCCAAC CCATCAGCCA 
ATCACTCTCA CCTCTGAGCA GGTCCAGGCC CTGGGTATCA CTCCAACCCA TCAGCCAATC 
ACTCTCACCC CTGAGCAGGC CCAGGCCCTG GCACTCATTC TCACCACTGA GCAGGTCAAG 
ACTCAGAGAA TCAATCTGAG CCCTGATCAG ACCCAGGCCC TGGGTATCAC CCCGACCCCT 
CAGCCGATCA CTTTCACCCC TGAGCAGACC CAGGCTCTGG GTATCACCCC AACCCCTCAG 
CTGATCACAC TAACCCCTGA GCAGGCCAAG GCCTTGGCGA ACACTCTCAC CGCTGAGCAG 
GTCAGTCTGA GCCCTCAACA GGCTGAGGCC CTGGGTATCA CTCCGACCCC TCAGCCGACC 
ACTCTCACCC CTGAGCAGGC CCAGGCCCTG GGTATCACTC CTACCCCACA GCCGATCACA 
CTAACCCCTG AGCAGGCCCA GGCCCTGGGC ATCACTCCGA CCCCTCAGCC GATCACACTA 
ACCCCTGAGC AGACCCAGGC CCTGGGTATC ACTCCTACCC CACAGCCGAT CACACTAACC 
CCTGAGCAGG CCCAGGCCCT GGGCATCACT CCGACCCCTC AGCCGATCAC ACTAACCCCT 
GAGCAGACCC AGGCCCTGGG TATCACTCCT ACCCCACAGC CGATCACACT AACCCCTGAG 
CAGGCCCAGG CCCTGGGCAT CACTCCGACC CCTCAGCCGA TCACACTAAC CCCTGAGCAG 
GCCCAGGCCC TGGGCATCAC TCCGACCCCT CAGCCGATCA CACTAACCCC TGAGCAGGCC 
CAGGCCCTGG GCATCACTCC GACCCCTCAG CCGATCACAC TAACCCCTGA GCAGACCCAG 
GCCCTGGGCA TCACTCCGAC CCCTCAGCCG ATCACACTAA CCCCTGAGCA GGCCCAGGCC 
CTGGGCATCA CTCCGACCCC TCAGCCGATC ACACTAACCC CTGAGCAGGT CCAGGCCCTG 
GGCATCACTC CGACCCCTCA GCCGATCACA CTAACCCCTG AGCAGGCCCA GGCCCTGGGT 
ATCACTCCTA CCCCACAGCC GATCACACTA ACCCCTGAGC AGGCCCAGGC CCTGGGCATC 
ACTCCGACCC CTCAGCCGAT CACACTAACC CCTGAGCAGA CCCAGGCCCT GGGTATCACT 
CCTACCCCAC AGCCGATCAC ACTAACCCCT GAGCAGGCCC AGGCCCTGGG CATCACTCCT 
ACCCCTCAGC CGATCACACT AACCCCTGAG CTGGTCCAGG CCCTGGGCAT CACTCCGACC 
CCTCAGCCGA TCACACTAAC CCCTGAGCAG GCCCAGGCCC TGGGCATCAC TCCGACCCCT 
CAGCCGACCA CTCTCAGCCC TGAACAGGCC CAGGCCCTGG GTATCACTCC GACCCCTCAG 
CCGATCACAC TAACCCCTGA GCAGGCCCAG GCCCTGGGCA TCACTCCGAC CCCTCAGCCG 
ACCACTCTCA GCCCTGAACA GGCCCAGGCT CTGGGGATTT CTCTCATTCC TAAACAACAG 
GAGATCAGTC TTAGTCCAGA GCAGGCCCAA GCTCTGGGGC TCACTCTCAC CCCTCAGCAG 
GCCCAGGTAC AAAAGATATA TCTCACTCCT CAGCAGGCCC AGGCTCTGGG AATCACTGTC 
AGCCCTGAGC AGGCCAAGGC CAAGGGGATC AGTCTGACCC CTGAGGAGGC CCACTCCCTA 
GGGATAATTC TCACAGTTGA GCAGGCCAAG GCAAAGAGGA TTAATTTGAC CCCTCAACAA 
GCTCAGGACC TGGGGCTCAC TCTCACCCCT GAGCAGGCCC AGGATCTGGG GATTTCTCTC 
ATTCCCAAAC AAGAGATCAG TTTTAGTCCC CTGCAGGCCC AAGCAATGGG GCTCACTCTC 
ACCCCTCAGC AGGCCCAGGT ACAAAAGATA TATCTCACTC CTCAGCAGGC CCAGGCTCTG 
GGAATCACTG TCAGCCCTGA GCAGGCAAAG GGGATCAGTC TGACCCCTGA GGAGGCCCAC 
TCCCTAGGGA TAATTCTCAC AGTTGAGCAG GCCAAGGCAC AGAGGATTAA TTTGACCCCT 
CAACAAGCTC AGGACCTGGG GCTCACTCTC ACCCCTGAGC AGGCCCAGGA TCTGGGGATT 
TCTCTCATTC CCAAACAACA AGAGATCAGT TTTAGTCCCC TGCAGGCCCA AGCAATGGGG 
CTCACTCTCA CCCCTCAGCA GGCCCAGGTA CAAAAGATAT ATCTCACTCC TCAGCAGGCC 
CAGGCTCTGG GAATCACTGT CAGCCCTGAG CAGGCAAAGG GGATCAGTCT GACCCCTGAG 
GAGGCCCACT CCCTAGGGAT AATTCTCACA GTTGAGCAGG CCAAGGCACA GAGGATTAAT 
TTGACCCCTC AACAAGCTCA GGACCTGGGG ATCACTCTCA CCCCTGAGCA GGCCCAGGAT 
CTGGGGATTT CTCTCATTCC CAAACAACAA GAGATCAGTT TTAGTCCCCT GCAGGCCCAA 
GCAATGGGGC TCACTCTCAC CCCTCAGCAG GCCCAGGTAC AAAAGATATA TCTCACTCCT 
CAGCAGGCCC AGGCTCTGGG AATCACTGTC AGCCCTGAGC AGGCAAAGGG GATCAGTCTG 
ACCCCTGAGG AGGCCCACTC CCTGGGAATC GCTCTCACAG TTGAGCAGGC CAAGGCACAG 
AGGATTAATC TGACCCCTCA GCAAGCTCAG GACCTGGGGC TCACTCTCAC CCCTGAGCAG 
GCCCAGGATC TGGGAATAGC TTCTACCCTT AAGCAGGCTG AAGCTGTGGG AATCATTCCC 
ACCCCTACCC CTGAGCCTTA CCAGGAAAGA CGTTTAAGTC TGACCTCTGA GCAGGTTCAG 
GCCTTGAGGA CCAGTTTCCC CACTAAGGAG ACCCTGTCAT TGGGGATTTA TCTCACCCCT 
AAGCAGGCCC AGTACCTGGG AATCACTCTC ACTGCTAGTC AAGCCAAGGT AATGAAGATT 
TGCCTCACCC CTGAGCAGGC CCAGGCCCTG GGGATCACTG TCACCCCTAA GCAGGCCAAG 
GCACGGAGGA CCAGTCTGAC CCCTGAGCAG GCCCAGGCTC TGGGAGTCAT TCTTACTCCT 
GAACAGGCCC AGGCCCACAG AATCACAGTC ACTCCTGAGC AGGCCCAGGC TCTGGGGATT 
GCTCTCACAC CTGAGCAGGC CCATGCCCTG GGGATTGCTC TCACACCTGA GCAGGCCCAG 
GCACAAGGTA TTTTTTCTCC TAAGAAATTC CAGGAATTGG ATGTTCCATT AACCTATAGA 
CAAACAAAAG CACTAAGGAG CCATCTTTCT AAGAAGCAAC TTGAAGCCCA GAGAAGTCAA 
ATCCTTATCT CTGAGTCTAT CCAAATCCCC AAACCAGCTG TCGCCTCTAA ATATACCCAG 
ACAATTGAAA TCCCACTAAC TACCCAACCA TTCCAGGCAT TTGGAGCTCT ACTTTCAAAG 
ACAAAGGAGA CAGAAATGGG AATCTCTCCC CTCACTCCCA AGCAGCCCCA GGCAATTGTA 
ACTCCACCTT CAAAGAGAAA GGAGAAAGAA TTGGGAATCT CTCCCCTCAC TCCCAAGCAG 
CCCCAGGCAG TTGAGCCTGC CAAGGCAAAG CTGCCACCCC TCACACCCAG TCAGGCCCAG 
CCTTTGCAGA AGCAGCTTGC TCCAGAGCTC ACACAAACAC TGCTGTTCAC CATCACTCTC 
CAGAAAGCCC AGCACTTGGG TGTCACTTTT ACCTACGAAC AAACTCAGGC AGCAGCTGTC 
ACCCTCACCT CTGAGCAGGT GGCAGCACTA GAAGACGCTC TCACTGAGAA TCTGGCTTGG 
AGATGGGAGA TTTCAGTCAC ACCAGGAATG GCTCAAGAGG CACCAAACAT CACTACCACT 
AAGCAACTGC AAGCCCTGGG GATTACTGCT CGCCAGCCAG CTCAGGCATT TCCCAGCCCT 
TTTACCTTGG AGAAGCCTGC CACGTTGGCT ACTTCCACCG ACAGACTATC TCAAAGATGG 
AAGGATTCTT ACCCTGCTTC TATTCCACTG CAGGCATTGA GGCCTTCTCC TACTCAAGCC 
CCATTTACCC CTACGACAAG CCTAGGAATA GGTAGTCTTC TGGACTCTGA GAAGCCATGG 
ATGTCTCCTA CCTACAGACA GACTCTCACA GACAGGGGCC AAGACGTTCT TGCACAGCCC 
CTAGCACCAG AAACCCCTCC TTCCCTCAGA CAGCTCCTAG CACCTGGGGC TCCTCCCACT 
CCAGGGCCAC CCCTTGGTCC TAGGCATTTC TTCAAACCTA GAGTCCCACC CACCTCTGGA 
GAGGTGCCAG GACTTGTTTC TGGAGGCTCT GCTGCCCATG AGGAGCTCCC AATGTCCAGA 
ACCACCCCCC TCCAGCCTCC TGAATGGCAA GGCCCATCAA GGCTCATTCC CGAGCAAGGC 
TTCATGCCAG CAATCTCTTC CATCCCACTG CATCCTTTCA CAGCAGAGGC CCTACCAACC 
CCTGGAAGAC CTCAGAGAAG TTCAAAAGCC AAACCTCTAA AACCTAAATC AGCAAGAGGT 
CTCCCAAATG TCACTCTGGG TTTTGAGACC TCTCAGGCTC CATTCCCTAT TGAGAAGACC 
CAAATACCTA AGACTCCTGA CACTTCAGAA CAAACTCAAG CACTCCAAGA TGCCTTGGGT 
GTGCAACCAT TTGGAATATT TCAGCCTTAT GGCACCAGCT CCGGGATAGC TCGGTCACAG 
TCCCCTCTCA TTGATGAGAA AGCCCTTTCT AGAGAGAAGC CTGGAACACC ACTACCATCT 
CTTACCACTC AACTCCCCCA AACACCACAG ATCTCAACTT CTGAAAAGGG CCAGAAACCA 
TGGCTCCCGC CGATAGACAA GCCCTGGACA CCAACCCCTG TTTCTAGTAC CAGAGAAGCC 
AAGATGATAG TGTCCCCCAC TGATCAGCAC CCTGAAGACG GCTATGTCGT CGATGTGGAG 
GCTCAGAGGA AGAACCTGGT CACATTAAAT CAGGCGGCAC AAACTTCTGC ACTCCCTGCA 
CAGTATCTCA CAATTGCCAA GAATCTCATA ATCGAATTAC TTCATATAGA TACAGTTCGG 
CTGGGGTATC TGTCCCGAAA GTATGTTGCC TATAGGCTGA TCCAGCTTGC CAGAAACCAC 
CTAACCAAAC GAGTGAAAAC CATCCAAAAC ACTGGGAAAG GGTACGAGGT TCAGAGCTTG 
TACACGATGC TTGATAGGAT TGACCAGTAC CAGAAGAAGG TGATGCACAG CTGGACGGAC 
AAGCAGAAGC AGCTGGAACA GAGACGAAAG CAGTGCCTAC GGTCTATGAC GCAGTTCTTC 
AGCCAGCTTG AAAGGGGATA TAAACTCAGT CTTAGCCAGC CCATGCCTTC GATCCCCAGC 
TTTAAGAAGA TACCCGGATT TACCAAACTT CAGCGGCCGG TCTTGGAGTT ATTAATTGAT 
GACAGCAAAA GATCTGACCT CTTCAAAACA CTCGGACAAG CTTCAGTGGA GGCCGTGTGG 
AACGCTGACC TGTCTACGTC AAGCTACCCA ATAATAGAGA AGGCACCGAT GAGTGCGCTC 
TGGGCCCAGC TGGGTGGGTA CCCAGATATT CCCAAGCTGC TGCAGTTAGA CATTCAGTCG 
ACCTTCAGAA AATCTCTTGC TTCCATCCGG TCACAGTCTA AGAAGATTCG TAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fam186a ( XM_030248787.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030104647.1 Mus musculus family with sequence similarity 186, member A (Fam186a), transcript variant X3, mRNA.

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CloneID	OMu86795
Clone ID Related Accession (Same CDS sequence)	XM_030248787.1
Accession Version	XM_030248787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8604bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	protein FAM186A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGACT ACTACCACTG GACTGTGAAG ATGGAGCTGA TACCAGACAC GCTGAAAAGG 
ATCAGCAAGA ATGTCGACTC TCTGATTCAA ATGGCCTTAT TACTCGTGGA AGAGAAGAAG 
AGAGCGAAGA AAAGAATATT GGCTAGAGGC ACTCTCTGGA AAGCTTGGAA AGACCGAGCT 
ATAAAGCGGC CTGCCACAGC CCAAGCTTTA AGACTAGATC AGATGATTTT TGACCAGATC 
GGCTTAAACG CTAAGGTTTC AGAGATTCAG GGCATGCTGC AGGAGCTCAT CGGCACAGCG 
ATGTTCAGCA AGCTGGAAAA TACTGCCATT AAATACATGT CCACCACAGT CATCAATCTC 
TCCAAGGCCC TGAACACCGT CAGCGATGAA CTAAAACTCG TGAGCTCTCG AGTGGTCAAG 
TCTGTTCTGA AGGAAGAGCA AGAGGAAGAT AAGGAGAAAG ACCGCTCCAA GCAGGTCATC 
CAAGAACTCA GCGAGGAGAA CGAGATGCTC CAGCAGAAAC TTAGAGAATC CGAAGAAAAA 
TGCGACCAAC TTATTCGCAT CAAAAATTAT CTTGGACGCC AGATGATACA CCCGGCTGGG 
TCCTTGAAAA TACTGCCTTT ACAGGGGCCG TTTTTCAGCA GGGAGTCCAG GGACTCTGAG 
AGGGAAGGAA AGACAGACGA CTCCCAGAAG GAAGGGACCA AAAGCTCCAC AGTGACATGG 
GACCCATCTT CCAGTGAAGG GGCTCCGGAT GCAGCTAAGC AGCAAGTTGA GGACAAAGCT 
GAGGCCCCCC AAGACCAGAT AAAACTCAAA GGCCTGCCAC TGACAGAGGA GACCTTGGAC 
ACAAATCTGG AAACGGAGGA AAAGAAACTC ACTCAACTGG ATATTCTAAA AGCAATGGAC 
AAGAAGAAAG TGGGTAAAGC AAAGGCCGAG CAAGGCAGAG GGGGACCAAG TCCCATGTGG 
GAACAACTCA AGAAGGGCAG ATCTGAGCAG CTGATTCCAG GCAGCACAGG CTCACCAGAG 
AGTAGAGAGT CCACCCTTAA ATTAACAGAC AAAGAAGCCA AGAGTGAAAT AGATCTGCTT 
CAGCCTGAGG GTACCCCAGA GCTACAAAAA CCAGAAACAA AGTGGAAAAG ACTCATAGCA 
AAAACTGAAG AAACAAAACG GTCTGGTAAG TTACAACCCA AGACTTCTAA GGTGACTTCA 
GTAACCCCTG ATGAAGGTGA GCAGGGCAGT CTAGAATCAT TTCAAAGAGC CATCCTGGCC 
TTCCTGAGAG AGAAGACGAA TAATGTAGGA AAGGCTTTTG ATCCAAAGAG TATACGGAAG 
GAGGAGTCGT CACTGGGAAA AGCAGAAGTT GAAAATTTAA ACGTCATAAA GGCAAAAATA 
GAGGAATATT TCCAAAAAGT GGCTGAAACT GTGACTGTAG CCTTGAGGAC ATACAGAGAT 
GAAAGAAAGG TTCAACCTAA AGAGAAGCCC AAGAAACAGC CAAAGGGAGC ACTGTCACGG 
ATGCTTCCTA AGCAGGGGGC GGTCGGCGCC AAGTCTGAGG TTGGCAGAGT TCTCCTCAGT 
GAGATCACAG ACCCAGCAAT TAAGAAACTG GTACAGATGC TCTTAAATGA GATAGAGAGT 
GACTTCAAAT ACAAAACGAC GAAGGAACAG CCACAGGCGG CGGTGGCGGC AGCGGCAGCG 
GCAGCGGCTG TGCAGAAGGA AGAGCCAGGA CTGAGGACGA AGGACAAAAC GGAGCTGCTG 
GAGGTGTGGA TGCAAGCATT CAAAGACTCA CAAGGCCACC AGGTTCAGGA CCTGGGAGAT 
GCAGAGGATG CAGAAAAGCC GAAGTGGAGC GAGCCGGGTC ATCAGGACCT GTGGAAGGGT 
GTCAGCAAGA CCTCCATCAA TCTAACTCCC AGGCTGATGA AGGCCCCGAA ACCGCAGATA 
AGTCAGGCGC AGGCGCTCCA GGTGGAGCCT GGTGTAATGG GGGCCACGGA GGAACAGCAG 
CCCAAACTCA CTCTCTCCTC TTCCAGAATC CCCTTCGTTT CTCCAAAGCC CCACCAAGTG 
CATGTCCCTG AGCAGGCCCA GATAATAGGC CCTCAGATGT CCCCTCAGCT TTCAATGGCA 
TCTCTCACCT CTGAGCAGGC ACAAACTTTG AGTGGTCCTC TTACTCATGA ACACACTATA 
GAATTAAAGA CTGCTTTCAC AGCAGGGCAG GAGCAAGAGC TTGGGGCTAA GCTGATGGTT 
CCTAGGATTC TTAAGCCGAC ACAGACTCCA AAGGAGTCTC TCATATCAGA AAAACCTGAG 
GCAGTGCAGA TCTCCCAAGT GACTACTGAA GAGATATCTG GAGTCACAGA TACCCAAAGA 
ACAATTCCAC CCTTCCAGCA GATCCTGGAA CAAGAAACCA CTCCCACTCC TGAGCAAGAC 
AAGGAACCAA GGATTACTCT CACCCGTCAG CAGACTGAGG CCCTAGGTAT CACTCTCACC 
CCTGAGCAAA CTAAGTCCCA GAGGATTAGT CTAACCCATG AGCAGGCTCG GGCCCTAGGG 
GTCACTCTCA CTCCTGAGCA GTACAAAGAG GACAGAATCA GTCTGACTCC CCTACAGGCC 
CAGGTCCTGG GAATCACTCT CAACCTTCAG CAGGCTAAGG CCCTGGGGAT CACTCTCACT 
CCTGAGCAAG TCAAGGCACA GAGGGTCAAT CTGATCCCTC AACAGTACCA GGTCCACGGA 
ACCACTCTCA CCACTCAGCA GGCTGAAGCA CAAAGAACTA ATCTCACGCC CGAGCAAGCC 
AAGGCCCTGG GTCTCCCTCT GATCCCTCCT AAGCCAATCA CTTTCACCCG TGAGCAGACC 
CAGGCCCTGG GTATCACTCC AACCCATCAG CCAATCACTC TCACCTCTGA GCAGGTCCAG 
GCCCTGGGTA TCACTCCAAC CCATCAGCCA ATCACTCTCA CCCCTGAGCA GGCCCAGGCC 
CTGGCACTCA TTCTCACCAC TGAGCAGGTC AAGACTCAGA GAATCAATCT GAGCCCTGAT 
CAGACCCAGG CCCTGGGTAT CACCCCGACC CCTCAGCCGA TCACTTTCAC CCCTGAGCAG 
ACCCAGGCTC TGGGTATCAC CCCAACCCCT CAGCTGATCA CACTAACCCC TGAGCAGGCC 
AAGGCCTTGG CGAACACTCT CACCGCTGAG CAGGTCAGTC TGAGCCCTCA ACAGGCTGAG 
GCCCTGGGTA TCACTCCGAC CCCTCAGCCG ACCACTCTCA CCCCTGAGCA GGCCCAGGCC 
CTGGGTATCA CTCCTACCCC ACAGCCGATC ACACTAACCC CTGAGCAGGC CCAGGCCCTG 
GGCATCACTC CGACCCCACA GCCGACCACA CTAACCCCTG AGCAGACCCA GGCCCTGGGT 
ATCACTCCTA CCCCACAGCC GATCACACTA ACCCCTGAGC AGGCCCAGGC CCTGGGCATC 
ACTCCGACCC CTCAGCCGAT CACACTAACC CCTGAGCAGA CCCAGGCCCT GGGTATCACT 
CCTACCCCAC AGCCGATCAC ACTAACCCCT GAGCAGGCCC AGGCCCTGGG CATCACTCCG 
ACCCCTCAGC CGATCACACT AACCCCTGAG CAGACCCAGG CCCTGGGTAT CACTCCTACC 
CCACAGCCGA TCACACTAAC CCCTGAGCAG GCCCAGGCCC TGGGCATCAC TCCGACCCCT 
CAGCCGATCA CACTAACCCC TGAGCAGGCC CAGGCCCTGG GCATCACTCC GACCCCTCAG 
CCGATCACAC TAACCCCTGA GCAGGCCCAG GCCCTGGGCA TCACTCCGAC CCCTCAGCCG 
ATCACACTAA CCCCTGAGCA GACCCAGGCC CTGGGCATCA CTCCGACCCC TCAGCCGATC 
ACACTAACCC CTGAGCAGGC CCAGGCCCTG GGCATCACTC CGACCCCTCA GCCGATCACA 
CTAACCCCTG AGCAGGTCCA GGCCCTGGGC ATCACTCCGA CCCCTCAGCC GATCACACTA 
ACCCCTGAGC AGGCCCAGGC CCTGGGTATC ACTCCTACCC CACAGCCGAT CACACTAACC 
CCTGAGCAGG CCCAGGCCCT GGGCATCACT CCGACCCCTC AGCCGATCAC ACTAACCCCT 
GAGCAGACCC AGGCCCTGGG TATCACTCCT ACCCCACAGC CGATCACACT AACCCCTGAG 
CAGGCCCAGG CCCTGGGCAT CACTCCTACC CCTCAGCCGA TCACACTAAC CCCTGAGCTG 
GTCCAGGCCC TGGGCATCAC TCCGACCCCT CAGCCGATCA CACTAACCCC TGAGCAGGCC 
CAGGCCCTGG GCATCACTCC GACCCCTCAG CCGACCACTC TCAGCCCTGA ACAGGCCCAG 
GCCCTGGGTA TCACTCCGAC CCCTCAGCCG ATCACACTAA CCCCTGAGCA GGCCCAGGCC 
CTGGGCATCA CTCCGACCCC TCAGCCGACC ACTCTCAGCC CTGAACAGGC CCAGGCTCTG 
GGGATTTCTC TCATTCCTAA ACAACAGGAG ATCAGTCTTA GTCCAGAGCA GGCCCAAGCT 
CTGGGGCTCA CTCTCACCCC TCAGCAGGCC CAGGTACAAA AGATATATCT CACTCCTCAG 
CAGGCCCAGG CTCTGGGAAT CACTGTCAGC CCTGAGCAGG CCAAGGCCAA GGGGATCAGT 
CTGACCCCTG AGGAGGCCCA CTCCCTAGGG ATAATTCTCA CAGTTGAGCA GGCCAAGGCA 
AAGAGGATTA ATTTGACCCC TCAACAAGCT CAGGACCTGG GGCTCACTCT CACCCCTGAG 
CAGGCCCAGG ATCTGGGGAT TTCTCTCATT CCCAAACAAG AGATCAGTTT TAGTCCCCTG 
CAGGCCCAAG CAATGGGGCT CACTCTCACC CCTCAGCAGG CCCAGGTACA AAAGATATAT 
CTCACTCCTC AGCAGGCCCA GGCTCTGGGA ATCACTGTCA GCCCTGAGCA GGCAAAGGGG 
ATCAGTCTGA CCCCTGAGGA GGCCCACTCC CTAGGGATAA TTCTCACAGT TGAGCAGGCC 
AAGGCACAGA GGATTAATTT GACCCCTCAA CAAGCTCAGG ACCTGGGGCT CACTCTCACC 
CCTGAGCAGG CCCAGGATCT GGGGATTTCT CTCATTCCCA AACAACAAGA GATCAGTTTT 
AGTCCCCTGC AGGCCCAAGC AATGGGGCTC ACTCTCACCC CTCAGCAGGC CCAGGTACAA 
AAGATATATC TCACTCCTCA GCAGGCCCAG GCTCTGGGAA TCACTGTCAG CCCTGAGCAG 
GCAAAGGGGA TCAGTCTGAC CCCTGAGGAG GCCCACTCCC TAGGGATAAT TCTCACAGTT 
GAGCAGGCCA AGGCACAGAG GATTAATTTG ACCCCTCAAC AAGCTCAGGA CCTGGGGATC 
ACTCTCACCC CTGAGCAGGC CCAGGATCTG GGGATTTCTC TCATTCCCAA ACAACAAGAG 
ATCAGTTTTA GTCCCCTGCA GGCCCAAGCA ATGGGGCTCA CTCTCACCCC TCAGCAGGCC 
CAGGTACAAA AGATATATCT CACTCCTCAG CAGGCCCAGG CTCTGGGAAT CACTGTCAGC 
CCTGAGCAGG CAAAGGGGAT CAGTCTGACC CCTGAGGAGG CCCACTCCCT GGGAATCGCT 
CTCACAGTTG AGCAGGCCAA GGCACAGAGG ATTAATCTGA CCCCTCAGCA AGCTCAGGAC 
CTGGGGCTCA CTCTCACCCC TGAGCAGGCC CAGGATCTGG GAATAGCTTC TACCCTTAAG 
CAGGCTGAAG CTGTGGGAAT CATTCCCACC CCTACCCCTG AGCCTTACCA GGAAAGACGT 
TTAAGTCTGA CCTCTGAGCA GGTTCAGGCC TTGAGGACCA GTTTCCCCAC TAAGGAGACC 
CTGTCATTGG GGATTTATCT CACCCCTAAG CAGGCCCAGT ACCTGGGAAT CACTCTCACT 
GCTAGTCAAG CCAAGGTAAT GAAGATTTGC CTCACCCCTG AGCAGGCCCA GGCCCTGGGG 
ATCACTGTCA CCCCTAAGCA GGCCAAGGCA CGGAGGACCA GTCTGACCCC TGAGCAGGCC 
CAGGCTCTGG GAGTCATTCT TACTCCTGAA CAGGCCCAGG CCCACAGAAT CACAGTCACT 
CCTGAGCAGG CCCAGGCTCT GGGGATTGCT CTCACACCTG AGCAGGCCCA TGCCCTGGGG 
ATTGCTCTCA CACCTGAGCA GGCCCAGGCA CAAGGTATTT TTTCTCCTAA GAAATTCCAG 
GAATTGGATG TTCCATTAAC CTATAGACAA ACAAAAGCAC TAAGGAGCCA TCTTTCTAAG 
AAGCAACTTG AAGCCCAGAG AAGTCAAATC CTTATCTCTG AGTCTATCCA AATCCCCAAA 
CCAGCTGTCG CCTCTAAATA TACCCAGACA ATTGAAATCC CACTAACTAC CCAACCATTC 
CAGGCATTTG GAGCTCTACT TTCAAAGACA AAGGAGACAG AAATGGGAAT CTCTCCCCTC 
ACTCCCAAGC AGCCCCAGGC AATTGTAACT CCACCTTCAA AGAGAAAGGA GAAAGAATTG 
GGAATCTCTC CCCTCACTCC CAAGCAGCCC CAGGCAGTTG AGCCTGCCAA GGCAAAGCTG 
CCACCCCTCA CACCCAGTCA GGCCCAGCCT TTGCAGAAGC AGCTTGCTCC AGAGCTCACA 
CAAACACTGC TGTTCACCAT CACTCTCCAG AAAGCCCAGC ACTTGGGTGT CACTTTTACC 
TACGAACAAA CTCAGGCAGC AGCTGTCACC CTCACCTCTG AGCAGGTGGC AGCACTAGAA 
GACGCTCTCA CTGAGAATCT GGCTTGGAGA TGGGAGATTT CAGTCACACC AGGAATGGCT 
CAAGAGGCAC CAAACATCAC TACCACTAAG CAACTGCAAG CCCTGGGGAT TACTGCTCGC 
CAGCCAGCTC AGGCATTTCC CAGCCCTTTT ACCTTGGAGA AGCCTGCCAC GTTGGCTACT 
TCCACCGACA GACTATCTCA AAGATGGAAG GATTCTTACC CTGCTTCTAT TCCACTGCAG 
GCATTGAGGC CTTCTCCTAC TCAAGCCCCA TTTACCCCTA CGACAAGCCT AGGAATAGGT 
AGTCTTCTGG ACTCTGAGAA GCCATGGATG TCTCCTACCT ACAGACAGAC TCTCACAGAC 
AGGGGCCAAG ACGTTCTTGC ACAGCCCCTA GCACCAGAAA CCCCTCCTTC CCTCAGACAG 
CTCCTAGCAC CTGGGGCTCC TCCCACTCCA GGGCCACCCC TTGGTCCTAG GCATTTCTTC 
AAACCTAGAG TCCCACCCAC CTCTGGAGAG GTGCCAGGAC TTGTTTCTGG AGGCTCTGCT 
GCCCATGAGG AGCTCCCAAT GTCCAGAACC ACCCCCCTCC AGCCTCCTGA ATGGCAAGGC 
CCATCAAGGC TCATTCCCGA GCAAGGCTTC ATGCCAGCAA TCTCTTCCAT CCCACTGCAT 
CCTTTCACAG CAGAGGCCCT ACCAACCCCT GGAAGACCTC AGAGAAGTTC AAAAGCCAAA 
CCTCTAAAAC CTAAATCAGC AAGAGGTCTC CCAAATGTCA CTCTGGGTTT TGAGACCTCT 
CAGGCTCCAT TCCCTATTGA GAAGACCCAA ATACCTAAGA CTCCTGACAC TTCAGAACAA 
ACTCAAGCAC TCCAAGATGC CTTGGGTGTG CAACCATTTG GAATATTTCA GCCTTATGGC 
ACCAGCTCCG GGATAGCTCG GTCACAGTCC CCTCTCATTG ATGAGAAAGC CCTTTCTAGA 
GAGAAGCCTG GAACACCACT ACCATCTCTT ACCACTCAAC TCCCCCAAAC ACCACAGATC 
TCAACTTCTG AAAAGGGCCA GAAACCATGG CTCCCGCCGA TAGACAAGCC CTGGACACCA 
ACCCCTGTTT CTAGTACCAG AGAAGCCAAG ATGATAGTGT CCCCCACTGA TCAGCACCCT 
GAAGACGGCT ATGTCGTCGA TGTGGAGGCT CAGAGGAAGA ACCTGGTCAC ATTAAATCAG 
GCGGCACAAA CTTCTGCACT CCCTGCACAG TATCTCACAA TTGCCAAGAA TCTCATAATC 
GAATTACTTC ATATAGATAC AGTTCGGCTG GGGTATCTGT CCCGAAAGTA TGTTGCCTAT 
AGGCTGATCC AGCTTGCCAG AAACCACCTA ACCAAACGAG TGAAAACCAT CCAAAACACT 
GGGAAAGGGT ACGAGGTTCA GAGCTTGTAC ACGATGCTTG ATAGGATTGA CCAGTACCAG 
AAGAAGGTGA TGCACAGCTG GACGGACAAG CAGAAGCAGC TGGAACAGAG ACGAAAGCAG 
TGCCTACGGT CTATGACGCA GTTCTTCAGC CAGCTTGAAA GGGGATATAA ACTCAGTCTT 
AGCCAGCCCA TGCCTTCGAT CCCCAGCTTT AAGAAGATAC CCGGATTTAC CAAACTTCAG 
CGGCCGGTCT TGGAGTTATT AATTGATGAC AGCAAAAGAT CTGACCTCTT CAAAACACTC 
GGACAAGCTT CAGTGGAGGC CGTGTGGAAC GCTGACCTGT CTACGTCAAG CTACCCAATA 
ATAGAGAAGG CACCGATGAG TGCGCTCTGG GCCCAGCTGG GTGGGTACCC AGATATTCCC 
AAGCTGCTGC AGTTAGACAT TCAGTCGACC TTCAGAAAAT CTCTTGCTTC CATCCGGTCA 
CAGTCTAAGA AGATTCGTAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030104647.1
CDS	86..8689
Translation	MDDYYHWTVKMELIPDTLKRISKNVDSLIQMALLLVEEKKRAKKRILARGTLWKAWKDRAIKRPATAQALRLDQMIFDQIGLNAKVSEIQGMLQELIGTAMFSKLENTAIKYMSTTVINLSKALNTVSDELKLVSSRVVKSVLKEEQEEDKEKDRSKQVIQELSEENEMLQQKLRESEEKCDQLIRIKNYLGRQMIHPAGSLKILPLQGPFFSRESRDSEREGKTDDSQKEGTKSSTVTWDPSSSEGAPDAAKQQVEDKAEAPQDQIKLKGLPLTEETLDTNLETEEKKLTQLDILKAMDKKKVGKAKAEQGRGGPSPMWEQLKKGRSEQLIPGSTGSPESRESTLKLTDKEAKSEIDLLQPEGTPELQKPETKWKRLIAKTEETKRSGKLQPKTSKVTSVTPDEGEQGSLESFQRAILAFLREKTNNVGKAFDPKSIRKEESSLGKAEVENLNVIKAKIEEYFQKVAETVTVALRTYRDERKVQPKEKPKKQPKGALSRMLPKQGAVGAKSEVGRVLLSEITDPAIKKLVQMLLNEIESDFKYKTTKEQPQAAVAAAAAAAAVQKEEPGLRTKDKTELLEVWMQAFKDSQGHQVQDLGDAEDAEKPKWSEPGHQDLWKGVSKTSINLTPRLMKAPKPQISQAQALQVEPGVMGATEEQQPKLTLSSSRIPFVSPKPHQVHVPEQAQIIGPQMSPQLSMASLTSEQAQTLSGPLTHEHTIELKTAFTAGQEQELGAKLMVPRILKPTQTPKESLISEKPEAVQISQVTTEEISGVTDTQRTIPPFQQILEQETTPTPEQDKEPRITLTRQQTEALGITLTPEQTKSQRISLTHEQARALGVTLTPEQYKEDRISLTPLQAQVLGITLNLQQAKALGITLTPEQVKAQRVNLIPQQYQVHGTTLTTQQAEAQRTNLTPEQAKALGLPLIPPKPITFTREQTQALGITPTHQPITLTSEQVQALGITPTHQPITLTPEQAQALALILTTEQVKTQRINLSPDQTQALGITPTPQPITFTPEQTQALGITPTPQLITLTPEQAKALANTLTAEQVSLSPQQAEALGITPTPQPTTLTPEQAQALGITPTPQPITLTPEQAQALGITPTPQPTTLTPEQTQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQTQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQTQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQTQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQVQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQAQALGITPTPQPITLTPEQTQALGITPTPQPITLTPEQAQALGITPTPQPITLTPELVQALGITPTPQPITLTPEQAQALGITPTPQPTTLSPEQAQALGITPTPQPITLTPEQAQALGITPTPQPTTLSPEQAQALGISLIPKQQEISLSPEQAQALGLTLTPQQAQVQKIYLTPQQAQALGITVSPEQAKAKGISLTPEEAHSLGIILTVEQAKAKRINLTPQQAQDLGLTLTPEQAQDLGISLIPKQEISFSPLQAQAMGLTLTPQQAQVQKIYLTPQQAQALGITVSPEQAKGISLTPEEAHSLGIILTVEQAKAQRINLTPQQAQDLGLTLTPEQAQDLGISLIPKQQEISFSPLQAQAMGLTLTPQQAQVQKIYLTPQQAQALGITVSPEQAKGISLTPEEAHSLGIILTVEQAKAQRINLTPQQAQDLGITLTPEQAQDLGISLIPKQQEISFSPLQAQAMGLTLTPQQAQVQKIYLTPQQAQALGITVSPEQAKGISLTPEEAHSLGIALTVEQAKAQRINLTPQQAQDLGLTLTPEQAQDLGIASTLKQAEAVGIIPTPTPEPYQERRLSLTSEQVQALRTSFPTKETLSLGIYLTPKQAQYLGITLTASQAKVMKICLTPEQAQALGITVTPKQAKARRTSLTPEQAQALGVILTPEQAQAHRITVTPEQAQALGIALTPEQAHALGIALTPEQAQAQGIFSPKKFQELDVPLTYRQTKALRSHLSKKQLEAQRSQILISESIQIPKPAVASKYTQTIEIPLTTQPFQAFGALLSKTKETEMGISPLTPKQPQAIVTPPSKRKEKELGISPLTPKQPQAVEPAKAKLPPLTPSQAQPLQKQLAPELTQTLLFTITLQKAQHLGVTFTYEQTQAAAVTLTSEQVAALEDALTENLAWRWEISVTPGMAQEAPNITTTKQLQALGITARQPAQAFPSPFTLEKPATLATSTDRLSQRWKDSYPASIPLQALRPSPTQAPFTPTTSLGIGSLLDSEKPWMSPTYRQTLTDRGQDVLAQPLAPETPPSLRQLLAPGAPPTPGPPLGPRHFFKPRVPPTSGEVPGLVSGGSAAHEELPMSRTTPLQPPEWQGPSRLIPEQGFMPAISSIPLHPFTAEALPTPGRPQRSSKAKPLKPKSARGLPNVTLGFETSQAPFPIEKTQIPKTPDTSEQTQALQDALGVQPFGIFQPYGTSSGIARSQSPLIDEKALSREKPGTPLPSLTTQLPQTPQISTSEKGQKPWLPPIDKPWTPTPVSSTREAKMIVSPTDQHPEDGYVVDVEAQRKNLVTLNQAAQTSALPAQYLTIAKNLIIELLHIDTVRLGYLSRKYVAYRLIQLARNHLTKRVKTIQNTGKGYEVQSLYTMLDRIDQYQKKVMHSWTDKQKQLEQRRKQCLRSMTQFFSQLERGYKLSLSQPMPSIPSFKKIPGFTKLQRPVLELLIDDSKRSDLFKTLGQASVEAVWNADLSTSSYPIIEKAPMSALWAQLGGYPDIPKLLQLDIQSTFRKSLASIRSQSKKIRK

Target ORF information:

RefSeq Version	XM_030248787.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus family with sequence similarity 186, member A (Fam186a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030248787.1

ORF Insert Sequence:

ATGGATGACT ACTACCACTG GACTGTGAAG ATGGAGCTGA TACCAGACAC GCTGAAAAGG 
ATCAGCAAGA ATGTCGACTC TCTGATTCAA ATGGCCTTAT TACTCGTGGA AGAGAAGAAG 
AGAGCGAAGA AAAGAATATT GGCTAGAGGC ACTCTCTGGA AAGCTTGGAA AGACCGAGCT 
ATAAAGCGGC CTGCCACAGC CCAAGCTTTA AGACTAGATC AGATGATTTT TGACCAGATC 
GGCTTAAACG CTAAGGTTTC AGAGATTCAG GGCATGCTGC AGGAGCTCAT CGGCACAGCG 
ATGTTCAGCA AGCTGGAAAA TACTGCCATT AAATACATGT CCACCACAGT CATCAATCTC 
TCCAAGGCCC TGAACACCGT CAGCGATGAA CTAAAACTCG TGAGCTCTCG AGTGGTCAAG 
TCTGTTCTGA AGGAAGAGCA AGAGGAAGAT AAGGAGAAAG ACCGCTCCAA GCAGGTCATC 
CAAGAACTCA GCGAGGAGAA CGAGATGCTC CAGCAGAAAC TTAGAGAATC CGAAGAAAAA 
TGCGACCAAC TTATTCGCAT CAAAAATTAT CTTGGACGCC AGATGATACA CCCGGCTGGG 
TCCTTGAAAA TACTGCCTTT ACAGGGGCCG TTTTTCAGCA GGGAGTCCAG GGACTCTGAG 
AGGGAAGGAA AGACAGACGA CTCCCAGAAG GAAGGGACCA AAAGCTCCAC AGTGACATGG 
GACCCATCTT CCAGTGAAGG GGCTCCGGAT GCAGCTAAGC AGCAAGTTGA GGACAAAGCT 
GAGGCCCCCC AAGACCAGAT AAAACTCAAA GGCCTGCCAC TGACAGAGGA GACCTTGGAC 
ACAAATCTGG AAACGGAGGA AAAGAAACTC ACTCAACTGG ATATTCTAAA AGCAATGGAC 
AAGAAGAAAG TGGGTAAAGC AAAGGCCGAG CAAGGCAGAG GGGGACCAAG TCCCATGTGG 
GAACAACTCA AGAAGGGCAG ATCTGAGCAG CTGATTCCAG GCAGCACAGG CTCACCAGAG 
AGTAGAGAGT CCACCCTTAA ATTAACAGAC AAAGAAGCCA AGAGTGAAAT AGATCTGCTT 
CAGCCTGAGG GTACCCCAGA GCTACAAAAA CCAGAAACAA AGTGGAAAAG ACTCATAGCA 
AAAACTGAAG AAACAAAACG GTCTGGTAAG TTACAACCCA AGACTTCTAA GGTGACTTCA 
GTAACCCCTG ATGAAGGTGA GCAGGGCAGT CTAGAATCAT TTCAAAGAGC CATCCTGGCC 
TTCCTGAGAG AGAAGACGAA TAATGTAGGA AAGGCTTTTG ATCCAAAGAG TATACGGAAG 
GAGGAGTCGT CACTGGGAAA AGCAGAAGTT GAAAATTTAA ACGTCATAAA GGCAAAAATA 
GAGGAATATT TCCAAAAAGT GGCTGAAACT GTGACTGTAG CCTTGAGGAC ATACAGAGAT 
GAAAGAAAGG TTCAACCTAA AGAGAAGCCC AAGAAACAGC CAAAGGGAGC ACTGTCACGG 
ATGCTTCCTA AGCAGGGGGC GGTCGGCGCC AAGTCTGAGG TTGGCAGAGT TCTCCTCAGT 
GAGATCACAG ACCCAGCAAT TAAGAAACTG GTACAGATGC TCTTAAATGA GATAGAGAGT 
GACTTCAAAT ACAAAACGAC GAAGGAACAG CCACAGGCGG CGGTGGCGGC AGCGGCAGCG 
GCAGCGGCTG TGCAGAAGGA AGAGCCAGGA CTGAGGACGA AGGACAAAAC GGAGCTGCTG 
GAGGTGTGGA TGCAAGCATT CAAAGACTCA CAAGGCCACC AGGTTCAGGA CCTGGGAGAT 
GCAGAGGATG CAGAAAAGCC GAAGTGGAGC GAGCCGGGTC ATCAGGACCT GTGGAAGGGT 
GTCAGCAAGA CCTCCATCAA TCTAACTCCC AGGCTGATGA AGGCCCCGAA ACCGCAGATA 
AGTCAGGCGC AGGCGCTCCA GGTGGAGCCT GGTGTAATGG GGGCCACGGA GGAACAGCAG 
CCCAAACTCA CTCTCTCCTC TTCCAGAATC CCCTTCGTTT CTCCAAAGCC CCACCAAGTG 
CATGTCCCTG AGCAGGCCCA GATAATAGGC CCTCAGATGT CCCCTCAGCT TTCAATGGCA 
TCTCTCACCT CTGAGCAGGC ACAAACTTTG AGTGGTCCTC TTACTCATGA ACACACTATA 
GAATTAAAGA CTGCTTTCAC AGCAGGGCAG GAGCAAGAGC TTGGGGCTAA GCTGATGGTT 
CCTAGGATTC TTAAGCCGAC ACAGACTCCA AAGGAGTCTC TCATATCAGA AAAACCTGAG 
GCAGTGCAGA TCTCCCAAGT GACTACTGAA GAGATATCTG GAGTCACAGA TACCCAAAGA 
ACAATTCCAC CCTTCCAGCA GATCCTGGAA CAAGAAACCA CTCCCACTCC TGAGCAAGAC 
AAGGAACCAA GGATTACTCT CACCCGTCAG CAGACTGAGG CCCTAGGTAT CACTCTCACC 
CCTGAGCAAA CTAAGTCCCA GAGGATTAGT CTAACCCATG AGCAGGCTCG GGCCCTAGGG 
GTCACTCTCA CTCCTGAGCA GTACAAAGAG GACAGAATCA GTCTGACTCC CCTACAGGCC 
CAGGTCCTGG GAATCACTCT CAACCTTCAG CAGGCTAAGG CCCTGGGGAT CACTCTCACT 
CCTGAGCAAG TCAAGGCACA GAGGGTCAAT CTGATCCCTC AACAGTACCA GGTCCACGGA 
ACCACTCTCA CCACTCAGCA GGCTGAAGCA CAAAGAACTA ATCTCACGCC CGAGCAAGCC 
AAGGCCCTGG GTCTCCCTCT GATCCCTCCT AAGCCAATCA CTTTCACCCG TGAGCAGACC 
CAGGCCCTGG GTATCACTCC AACCCATCAG CCAATCACTC TCACCTCTGA GCAGGTCCAG 
GCCCTGGGTA TCACTCCAAC CCATCAGCCA ATCACTCTCA CCCCTGAGCA GGCCCAGGCC 
CTGGCACTCA TTCTCACCAC TGAGCAGGTC AAGACTCAGA GAATCAATCT GAGCCCTGAT 
CAGACCCAGG CCCTGGGTAT CACCCCGACC CCTCAGCCGA TCACTTTCAC CCCTGAGCAG 
ACCCAGGCTC TGGGTATCAC CCCAACCCCT CAGCTGATCA CACTAACCCC TGAGCAGGCC 
AAGGCCTTGG CGAACACTCT CACCGCTGAG CAGGTCAGTC TGAGCCCTCA ACAGGCTGAG 
GCCCTGGGTA TCACTCCGAC CCCTCAGCCG ACCACTCTCA CCCCTGAGCA GGCCCAGGCC 
CTGGGTATCA CTCCTACCCC ACAGCCGATC ACACTAACCC CTGAGCAGGC CCAGGCCCTG 
GGCATCACTC CGACCCCACA GCCGACCACA CTAACCCCTG AGCAGACCCA GGCCCTGGGT 
ATCACTCCTA CCCCACAGCC GATCACACTA ACCCCTGAGC AGGCCCAGGC CCTGGGCATC 
ACTCCGACCC CTCAGCCGAT CACACTAACC CCTGAGCAGA CCCAGGCCCT GGGTATCACT 
CCTACCCCAC AGCCGATCAC ACTAACCCCT GAGCAGGCCC AGGCCCTGGG CATCACTCCG 
ACCCCTCAGC CGATCACACT AACCCCTGAG CAGACCCAGG CCCTGGGTAT CACTCCTACC 
CCACAGCCGA TCACACTAAC CCCTGAGCAG GCCCAGGCCC TGGGCATCAC TCCGACCCCT 
CAGCCGATCA CACTAACCCC TGAGCAGGCC CAGGCCCTGG GCATCACTCC GACCCCTCAG 
CCGATCACAC TAACCCCTGA GCAGGCCCAG GCCCTGGGCA TCACTCCGAC CCCTCAGCCG 
ATCACACTAA CCCCTGAGCA GACCCAGGCC CTGGGCATCA CTCCGACCCC TCAGCCGATC 
ACACTAACCC CTGAGCAGGC CCAGGCCCTG GGCATCACTC CGACCCCTCA GCCGATCACA 
CTAACCCCTG AGCAGGTCCA GGCCCTGGGC ATCACTCCGA CCCCTCAGCC GATCACACTA 
ACCCCTGAGC AGGCCCAGGC CCTGGGTATC ACTCCTACCC CACAGCCGAT CACACTAACC 
CCTGAGCAGG CCCAGGCCCT GGGCATCACT CCGACCCCTC AGCCGATCAC ACTAACCCCT 
GAGCAGACCC AGGCCCTGGG TATCACTCCT ACCCCACAGC CGATCACACT AACCCCTGAG 
CAGGCCCAGG CCCTGGGCAT CACTCCTACC CCTCAGCCGA TCACACTAAC CCCTGAGCTG 
GTCCAGGCCC TGGGCATCAC TCCGACCCCT CAGCCGATCA CACTAACCCC TGAGCAGGCC 
CAGGCCCTGG GCATCACTCC GACCCCTCAG CCGACCACTC TCAGCCCTGA ACAGGCCCAG 
GCCCTGGGTA TCACTCCGAC CCCTCAGCCG ATCACACTAA CCCCTGAGCA GGCCCAGGCC 
CTGGGCATCA CTCCGACCCC TCAGCCGACC ACTCTCAGCC CTGAACAGGC CCAGGCTCTG 
GGGATTTCTC TCATTCCTAA ACAACAGGAG ATCAGTCTTA GTCCAGAGCA GGCCCAAGCT 
CTGGGGCTCA CTCTCACCCC TCAGCAGGCC CAGGTACAAA AGATATATCT CACTCCTCAG 
CAGGCCCAGG CTCTGGGAAT CACTGTCAGC CCTGAGCAGG CCAAGGCCAA GGGGATCAGT 
CTGACCCCTG AGGAGGCCCA CTCCCTAGGG ATAATTCTCA CAGTTGAGCA GGCCAAGGCA 
AAGAGGATTA ATTTGACCCC TCAACAAGCT CAGGACCTGG GGCTCACTCT CACCCCTGAG 
CAGGCCCAGG ATCTGGGGAT TTCTCTCATT CCCAAACAAG AGATCAGTTT TAGTCCCCTG 
CAGGCCCAAG CAATGGGGCT CACTCTCACC CCTCAGCAGG CCCAGGTACA AAAGATATAT 
CTCACTCCTC AGCAGGCCCA GGCTCTGGGA ATCACTGTCA GCCCTGAGCA GGCAAAGGGG 
ATCAGTCTGA CCCCTGAGGA GGCCCACTCC CTAGGGATAA TTCTCACAGT TGAGCAGGCC 
AAGGCACAGA GGATTAATTT GACCCCTCAA CAAGCTCAGG ACCTGGGGCT CACTCTCACC 
CCTGAGCAGG CCCAGGATCT GGGGATTTCT CTCATTCCCA AACAACAAGA GATCAGTTTT 
AGTCCCCTGC AGGCCCAAGC AATGGGGCTC ACTCTCACCC CTCAGCAGGC CCAGGTACAA 
AAGATATATC TCACTCCTCA GCAGGCCCAG GCTCTGGGAA TCACTGTCAG CCCTGAGCAG 
GCAAAGGGGA TCAGTCTGAC CCCTGAGGAG GCCCACTCCC TAGGGATAAT TCTCACAGTT 
GAGCAGGCCA AGGCACAGAG GATTAATTTG ACCCCTCAAC AAGCTCAGGA CCTGGGGATC 
ACTCTCACCC CTGAGCAGGC CCAGGATCTG GGGATTTCTC TCATTCCCAA ACAACAAGAG 
ATCAGTTTTA GTCCCCTGCA GGCCCAAGCA ATGGGGCTCA CTCTCACCCC TCAGCAGGCC 
CAGGTACAAA AGATATATCT CACTCCTCAG CAGGCCCAGG CTCTGGGAAT CACTGTCAGC 
CCTGAGCAGG CAAAGGGGAT CAGTCTGACC CCTGAGGAGG CCCACTCCCT GGGAATCGCT 
CTCACAGTTG AGCAGGCCAA GGCACAGAGG ATTAATCTGA CCCCTCAGCA AGCTCAGGAC 
CTGGGGCTCA CTCTCACCCC TGAGCAGGCC CAGGATCTGG GAATAGCTTC TACCCTTAAG 
CAGGCTGAAG CTGTGGGAAT CATTCCCACC CCTACCCCTG AGCCTTACCA GGAAAGACGT 
TTAAGTCTGA CCTCTGAGCA GGTTCAGGCC TTGAGGACCA GTTTCCCCAC TAAGGAGACC 
CTGTCATTGG GGATTTATCT CACCCCTAAG CAGGCCCAGT ACCTGGGAAT CACTCTCACT 
GCTAGTCAAG CCAAGGTAAT GAAGATTTGC CTCACCCCTG AGCAGGCCCA GGCCCTGGGG 
ATCACTGTCA CCCCTAAGCA GGCCAAGGCA CGGAGGACCA GTCTGACCCC TGAGCAGGCC 
CAGGCTCTGG GAGTCATTCT TACTCCTGAA CAGGCCCAGG CCCACAGAAT CACAGTCACT 
CCTGAGCAGG CCCAGGCTCT GGGGATTGCT CTCACACCTG AGCAGGCCCA TGCCCTGGGG 
ATTGCTCTCA CACCTGAGCA GGCCCAGGCA CAAGGTATTT TTTCTCCTAA GAAATTCCAG 
GAATTGGATG TTCCATTAAC CTATAGACAA ACAAAAGCAC TAAGGAGCCA TCTTTCTAAG 
AAGCAACTTG AAGCCCAGAG AAGTCAAATC CTTATCTCTG AGTCTATCCA AATCCCCAAA 
CCAGCTGTCG CCTCTAAATA TACCCAGACA ATTGAAATCC CACTAACTAC CCAACCATTC 
CAGGCATTTG GAGCTCTACT TTCAAAGACA AAGGAGACAG AAATGGGAAT CTCTCCCCTC 
ACTCCCAAGC AGCCCCAGGC AATTGTAACT CCACCTTCAA AGAGAAAGGA GAAAGAATTG 
GGAATCTCTC CCCTCACTCC CAAGCAGCCC CAGGCAGTTG AGCCTGCCAA GGCAAAGCTG 
CCACCCCTCA CACCCAGTCA GGCCCAGCCT TTGCAGAAGC AGCTTGCTCC AGAGCTCACA 
CAAACACTGC TGTTCACCAT CACTCTCCAG AAAGCCCAGC ACTTGGGTGT CACTTTTACC 
TACGAACAAA CTCAGGCAGC AGCTGTCACC CTCACCTCTG AGCAGGTGGC AGCACTAGAA 
GACGCTCTCA CTGAGAATCT GGCTTGGAGA TGGGAGATTT CAGTCACACC AGGAATGGCT 
CAAGAGGCAC CAAACATCAC TACCACTAAG CAACTGCAAG CCCTGGGGAT TACTGCTCGC 
CAGCCAGCTC AGGCATTTCC CAGCCCTTTT ACCTTGGAGA AGCCTGCCAC GTTGGCTACT 
TCCACCGACA GACTATCTCA AAGATGGAAG GATTCTTACC CTGCTTCTAT TCCACTGCAG 
GCATTGAGGC CTTCTCCTAC TCAAGCCCCA TTTACCCCTA CGACAAGCCT AGGAATAGGT 
AGTCTTCTGG ACTCTGAGAA GCCATGGATG TCTCCTACCT ACAGACAGAC TCTCACAGAC 
AGGGGCCAAG ACGTTCTTGC ACAGCCCCTA GCACCAGAAA CCCCTCCTTC CCTCAGACAG 
CTCCTAGCAC CTGGGGCTCC TCCCACTCCA GGGCCACCCC TTGGTCCTAG GCATTTCTTC 
AAACCTAGAG TCCCACCCAC CTCTGGAGAG GTGCCAGGAC TTGTTTCTGG AGGCTCTGCT 
GCCCATGAGG AGCTCCCAAT GTCCAGAACC ACCCCCCTCC AGCCTCCTGA ATGGCAAGGC 
CCATCAAGGC TCATTCCCGA GCAAGGCTTC ATGCCAGCAA TCTCTTCCAT CCCACTGCAT 
CCTTTCACAG CAGAGGCCCT ACCAACCCCT GGAAGACCTC AGAGAAGTTC AAAAGCCAAA 
CCTCTAAAAC CTAAATCAGC AAGAGGTCTC CCAAATGTCA CTCTGGGTTT TGAGACCTCT 
CAGGCTCCAT TCCCTATTGA GAAGACCCAA ATACCTAAGA CTCCTGACAC TTCAGAACAA 
ACTCAAGCAC TCCAAGATGC CTTGGGTGTG CAACCATTTG GAATATTTCA GCCTTATGGC 
ACCAGCTCCG GGATAGCTCG GTCACAGTCC CCTCTCATTG ATGAGAAAGC CCTTTCTAGA 
GAGAAGCCTG GAACACCACT ACCATCTCTT ACCACTCAAC TCCCCCAAAC ACCACAGATC 
TCAACTTCTG AAAAGGGCCA GAAACCATGG CTCCCGCCGA TAGACAAGCC CTGGACACCA 
ACCCCTGTTT CTAGTACCAG AGAAGCCAAG ATGATAGTGT CCCCCACTGA TCAGCACCCT 
GAAGACGGCT ATGTCGTCGA TGTGGAGGCT CAGAGGAAGA ACCTGGTCAC ATTAAATCAG 
GCGGCACAAA CTTCTGCACT CCCTGCACAG TATCTCACAA TTGCCAAGAA TCTCATAATC 
GAATTACTTC ATATAGATAC AGTTCGGCTG GGGTATCTGT CCCGAAAGTA TGTTGCCTAT 
AGGCTGATCC AGCTTGCCAG AAACCACCTA ACCAAACGAG TGAAAACCAT CCAAAACACT 
GGGAAAGGGT ACGAGGTTCA GAGCTTGTAC ACGATGCTTG ATAGGATTGA CCAGTACCAG 
AAGAAGGTGA TGCACAGCTG GACGGACAAG CAGAAGCAGC TGGAACAGAG ACGAAAGCAG 
TGCCTACGGT CTATGACGCA GTTCTTCAGC CAGCTTGAAA GGGGATATAA ACTCAGTCTT 
AGCCAGCCCA TGCCTTCGAT CCCCAGCTTT AAGAAGATAC CCGGATTTAC CAAACTTCAG 
CGGCCGGTCT TGGAGTTATT AATTGATGAC AGCAAAAGAT CTGACCTCTT CAAAACACTC 
GGACAAGCTT CAGTGGAGGC CGTGTGGAAC GCTGACCTGT CTACGTCAAG CTACCCAATA 
ATAGAGAAGG CACCGATGAG TGCGCTCTGG GCCCAGCTGG GTGGGTACCC AGATATTCCC 
AAGCTGCTGC AGTTAGACAT TCAGTCGACC TTCAGAAAAT CTCTTGCTTC CATCCGGTCA 
CAGTCTAAGA AGATTCGTAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A high-resolution anatomical atlas of the transcriptome in the mouse embryo.
PLoS biology9(1)e1000582(2011 Jan)
Diez-Roux G,Banfi S,Sultan M,Geffers L,Anand S,Rozado D,Magen A,Canidio E,Pagani M,Peluso I,Lin-Marq N,Koch M,Bilio M,Cantiello I,Verde R,De Masi C,Bianchi SA,Cicchini J,Perroud E,Mehmeti S,Dagand E,Schrinner S,Nürnberger A,Schmidt K,Metz K,Zwingmann C,Brieske N,Springer C,Hernandez AM,Herzog S,Grabbe F,Sieverding C,Fischer B,Schrader K,Brockmeyer M,Dettmer S,Helbig C,Alunni V,Battaini MA,Mura C,Henrichsen CN,Garcia-Lopez R,Echevarria D,Puelles E,Garcia-Calero E,Kruse S,Uhr M,Kauck C,Feng G,Milyaev N,Ong CK,Kumar L,Lam M,Semple CA,Gyenesei A,Mundlos S,Radelof U,Lehrach H,Sarmientos P,Reymond A,Davidson DR,Dollé P,Antonarakis SE,Yaspo ML,Martinez S,Baldock RA,Eichele G,Ballabio A

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Fam186a cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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