Zfp422 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Zfp422
Entrez Gene ID	67255
Full Name	zinc finger protein 422
Synonyms	2900028O21Rik,AU041373,KOX15,Krox-25-2,Krox-26,Krox25,Krox26,Znf22
General protein information	Preferred Names zinc finger protein 422 Names zinc finger protein 22 Kruppel type zinc finger protein Krox26 krox-25 protein zinc finger protein Krox-25 zinc finger protein Krox-26
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 E3
Summary	This gene encodes a putative C2H2 zinc finger transcription factor that may play a role in tooth development. A pseudogene related to this gene is located on chromosome 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001302439.1	NP_001289368.1	zinc finger protein 22
NM_001302440.1	NP_001289369.1	zinc finger protein 22
NM_026057.3	NP_080333.1	zinc finger protein 22
XM_030255555.1	XP_030111415.1	zinc finger protein 22 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ZNF22	XP_521459.1
Macaca mulatta (Rhesus monkey)	ZNF22	XP_001101436.1
Mus musculus (house mouse)	Zfp422	NP_080333.1
Homo sapiens (human)	ZNF22	NP_008894.2
Canis lupus familiaris (dog)	ZNF22	XP_003433683.1
Bos taurus (cattle)	ZNF22	NP_001070576.1
Rattus norvegicus (Norway rat)	Zfp422	NP_001012763.1

Gene Ontology
Bibliography

Related articles in PubMed

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Tbx22 expressions during palatal development in fetuses with glucocorticoid-/alcohol-induced C57BL/6N cleft palates.
Kim SM, Lee JH, Jabaiti S, Lee SK, Choi JY
The Journal of craniofacial surgery20(5)1316-26(2009 Sep)

Molecular cloning, chromosomal mapping, and characteristic expression in tooth organ of rat and mouse Krox-25.
Lee SK, Kim YS, Lee SS, Lee YJ, Song IS, Park SC, Kozak C, Yamada Y
Genomics83(2)243-53(2004 Feb)

The human KROX-26/ZNF22 gene is expressed at sites of tooth formation and maps to the locus for permanent tooth agenesis (He-Zhao deficiency).
Gao Y, Kobayashi H, Ganss B
Journal of dental research82(12)1002-7(2003 Dec)

Developmental expression pattern and DNA-binding properties of the zinc finger transcription factor Krox-26.
Teo W, Chen H, Poon T, Ganss B
Connective tissue research44 Suppl 1161-6(2003)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Zfp422 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Zfp422 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14970	NM_001302439.1 Latest version!	Mus musculus zinc finger protein 422 (Zfp422), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu14970	NM_026057.3 Latest version!	Mus musculus zinc finger protein 422 (Zfp422), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu14970	NM_001302440.1 Latest version!	Mus musculus zinc finger protein 422 (Zfp422), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu14970	XM_030255555.1 Latest version!	Mus musculus zinc finger protein 422 (Zfp422), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Zfp422 ( NM_001302439.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001289368.1 Mus musculus zinc finger protein 422 (Zfp422), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu14970
Clone ID Related Accession (Same CDS sequence)	NM_001302439.1 , NM_001302440.1 , NM_026057.3 , XM_030255555.1
Accession Version	NM_001302439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571673600000
Organism	Mus musculus(house mouse)
Product	zinc finger protein 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY074474.1, AK169251.1, AK152681.1 and AK082970.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGTTAG GAAAGCCCAA AGGGGGTATT TCTCGAAGCG CCAGCCAAGG AAAAGCCTAT 
GAGAGCAAGC GCAAGACAGC CCGCCAGCGA CAGAAATGGG GGGTGGCCAT TCGCTTCGAC 
TCGGGCTTGA GTCGACGGAG AAGGAACGTG GACGAGAAGC CTTACAAGTG TGCTAAATGC 
TCCAAGAGTT TCAGTCAGAG CTCCACGCTC TTTCAGCACA AGAAGATCCA TACTGGGAAG 
AAATCCCATA AATGTGCCGA TTGTGGGAAA AGTTTCTTTC AGAGTTCTAA CCTCATCCAG 
CACCGGCGGA TCCACACGGG GGAAAAGCCC TATAAGTGTG ACGAGTGTGG AGAGAGATTC 
AAGCAGAGCT CCAATCTCAT CCAGCACCAG AGGATCCACA CCGGCGAGAA GCCCTACTGC 
TGTGATGAGT GTGGCCGGTG CTTTAGCCAG AGTTCCCACC TCATTCAGCA CCAGAGAACC 
CACACCGGAG AGAAGCCCTA CCAGTGTGAG GAGTGTGACA AGTGCTTCAG CCAGAGCTCC 
CATCTGAGGC AGCACATGAA GGTGCACAAA GAAAAGAAGC CACATAAGAG GGGCAAAAAT 
GCCAGGGTGA AAACTCACCC GGTGTCCTGG AAAAGGGGCA AAGGGAGGAA GGCGGTGGCT 
GGGATCCGCC AGGTAAAGGG AGCCACTTCC GGCCTTTTTA AAAAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289368.1
CDS	249..962
Translation	MRLGKPKGGISRSASQGKAYESKRKTARQRQKWGVAIRFDSGLSRRRRNVDEKPYKCAKCSKSFSQSSTLFQHKKIHTGKKSHKCADCGKSFFQSSNLIQHRRIHTGEKPYKCDECGERFKQSSNLIQHQRIHTGEKPYCCDECGRCFSQSSHLIQHQRTHTGEKPYQCEECDKCFSQSSHLRQHMKVHKEKKPHKRGKNARVKTHPVSWKRGKGRKAVAGIRQVKGATSGLFKKKK

Target ORF information:

RefSeq Version	NM_001302439.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 422 (Zfp422), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302439.1

ORF Insert Sequence:

ATGAGGTTAG GAAAGCCCAA AGGGGGTATT TCTCGAAGCG CCAGCCAAGG AAAAGCCTAT 
GAGAGCAAGC GCAAGACAGC CCGCCAGCGA CAGAAATGGG GGGTGGCCAT TCGCTTCGAC 
TCGGGCTTGA GTCGACGGAG AAGGAACGTG GACGAGAAGC CTTACAAGTG TGCTAAATGC 
TCCAAGAGTT TCAGTCAGAG CTCCACGCTC TTTCAGCACA AGAAGATCCA TACTGGGAAG 
AAATCCCATA AATGTGCCGA TTGTGGGAAA AGTTTCTTTC AGAGTTCTAA CCTCATCCAG 
CACCGGCGGA TCCACACGGG GGAAAAGCCC TATAAGTGTG ACGAGTGTGG AGAGAGATTC 
AAGCAGAGCT CCAATCTCAT CCAGCACCAG AGGATCCACA CCGGCGAGAA GCCCTACTGC 
TGTGATGAGT GTGGCCGGTG CTTTAGCCAG AGTTCCCACC TCATTCAGCA CCAGAGAACC 
CACACCGGAG AGAAGCCCTA CCAGTGTGAG GAGTGTGACA AGTGCTTCAG CCAGAGCTCC 
CATCTGAGGC AGCACATGAA GGTGCACAAA GAAAAGAAGC CACATAAGAG GGGCAAAAAT 
GCCAGGGTGA AAACTCACCC GGTGTCCTGG AAAAGGGGCA AAGGGAGGAA GGCGGTGGCT 
GGGATCCGCC AGGTAAAGGG AGCCACTTCC GGCCTTTTTA AAAAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfp422 ( NM_001302440.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001289369.1 Mus musculus zinc finger protein 422 (Zfp422), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu14970
Clone ID Related Accession (Same CDS sequence)	NM_001302439.1 , NM_001302440.1 , NM_026057.3 , XM_030255555.1
Accession Version	NM_001302440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571414400000
Organism	Mus musculus(house mouse)
Product	zinc finger protein 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY059739.1, AK169251.1, AK152681.1 and AK082970.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGTTAG GAAAGCCCAA AGGGGGTATT TCTCGAAGCG CCAGCCAAGG AAAAGCCTAT 
GAGAGCAAGC GCAAGACAGC CCGCCAGCGA CAGAAATGGG GGGTGGCCAT TCGCTTCGAC 
TCGGGCTTGA GTCGACGGAG AAGGAACGTG GACGAGAAGC CTTACAAGTG TGCTAAATGC 
TCCAAGAGTT TCAGTCAGAG CTCCACGCTC TTTCAGCACA AGAAGATCCA TACTGGGAAG 
AAATCCCATA AATGTGCCGA TTGTGGGAAA AGTTTCTTTC AGAGTTCTAA CCTCATCCAG 
CACCGGCGGA TCCACACGGG GGAAAAGCCC TATAAGTGTG ACGAGTGTGG AGAGAGATTC 
AAGCAGAGCT CCAATCTCAT CCAGCACCAG AGGATCCACA CCGGCGAGAA GCCCTACTGC 
TGTGATGAGT GTGGCCGGTG CTTTAGCCAG AGTTCCCACC TCATTCAGCA CCAGAGAACC 
CACACCGGAG AGAAGCCCTA CCAGTGTGAG GAGTGTGACA AGTGCTTCAG CCAGAGCTCC 
CATCTGAGGC AGCACATGAA GGTGCACAAA GAAAAGAAGC CACATAAGAG GGGCAAAAAT 
GCCAGGGTGA AAACTCACCC GGTGTCCTGG AAAAGGGGCA AAGGGAGGAA GGCGGTGGCT 
GGGATCCGCC AGGTAAAGGG AGCCACTTCC GGCCTTTTTA AAAAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289369.1
CDS	391..1104
Translation	MRLGKPKGGISRSASQGKAYESKRKTARQRQKWGVAIRFDSGLSRRRRNVDEKPYKCAKCSKSFSQSSTLFQHKKIHTGKKSHKCADCGKSFFQSSNLIQHRRIHTGEKPYKCDECGERFKQSSNLIQHQRIHTGEKPYCCDECGRCFSQSSHLIQHQRTHTGEKPYQCEECDKCFSQSSHLRQHMKVHKEKKPHKRGKNARVKTHPVSWKRGKGRKAVAGIRQVKGATSGLFKKKK

Target ORF information:

RefSeq Version	NM_001302440.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 422 (Zfp422), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302440.1

ORF Insert Sequence:

ATGAGGTTAG GAAAGCCCAA AGGGGGTATT TCTCGAAGCG CCAGCCAAGG AAAAGCCTAT 
GAGAGCAAGC GCAAGACAGC CCGCCAGCGA CAGAAATGGG GGGTGGCCAT TCGCTTCGAC 
TCGGGCTTGA GTCGACGGAG AAGGAACGTG GACGAGAAGC CTTACAAGTG TGCTAAATGC 
TCCAAGAGTT TCAGTCAGAG CTCCACGCTC TTTCAGCACA AGAAGATCCA TACTGGGAAG 
AAATCCCATA AATGTGCCGA TTGTGGGAAA AGTTTCTTTC AGAGTTCTAA CCTCATCCAG 
CACCGGCGGA TCCACACGGG GGAAAAGCCC TATAAGTGTG ACGAGTGTGG AGAGAGATTC 
AAGCAGAGCT CCAATCTCAT CCAGCACCAG AGGATCCACA CCGGCGAGAA GCCCTACTGC 
TGTGATGAGT GTGGCCGGTG CTTTAGCCAG AGTTCCCACC TCATTCAGCA CCAGAGAACC 
CACACCGGAG AGAAGCCCTA CCAGTGTGAG GAGTGTGACA AGTGCTTCAG CCAGAGCTCC 
CATCTGAGGC AGCACATGAA GGTGCACAAA GAAAAGAAGC CACATAAGAG GGGCAAAAAT 
GCCAGGGTGA AAACTCACCC GGTGTCCTGG AAAAGGGGCA AAGGGAGGAA GGCGGTGGCT 
GGGATCCGCC AGGTAAAGGG AGCCACTTCC GGCCTTTTTA AAAAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfp422 ( NM_026057.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080333.1 Mus musculus zinc finger protein 422 (Zfp422), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14970
Clone ID Related Accession (Same CDS sequence)	NM_001302439.1 , NM_001302440.1 , NM_026057.3 , XM_030255555.1
Accession Version	NM_026057.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571673600000
Organism	Mus musculus(house mouse)
Product	zinc finger protein 22
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY082381.1, AK082970.1, AK169251.1 and AK152681.1. On Oct 22, 2014 this sequence version replaced NM_026057.2. Transcript Variant: This variant (1) represents the shortest transcript. Variants 1, 2 and 3 encode the same protein. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849388, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGTTAG GAAAGCCCAA AGGGGGTATT TCTCGAAGCG CCAGCCAAGG AAAAGCCTAT 
GAGAGCAAGC GCAAGACAGC CCGCCAGCGA CAGAAATGGG GGGTGGCCAT TCGCTTCGAC 
TCGGGCTTGA GTCGACGGAG AAGGAACGTG GACGAGAAGC CTTACAAGTG TGCTAAATGC 
TCCAAGAGTT TCAGTCAGAG CTCCACGCTC TTTCAGCACA AGAAGATCCA TACTGGGAAG 
AAATCCCATA AATGTGCCGA TTGTGGGAAA AGTTTCTTTC AGAGTTCTAA CCTCATCCAG 
CACCGGCGGA TCCACACGGG GGAAAAGCCC TATAAGTGTG ACGAGTGTGG AGAGAGATTC 
AAGCAGAGCT CCAATCTCAT CCAGCACCAG AGGATCCACA CCGGCGAGAA GCCCTACTGC 
TGTGATGAGT GTGGCCGGTG CTTTAGCCAG AGTTCCCACC TCATTCAGCA CCAGAGAACC 
CACACCGGAG AGAAGCCCTA CCAGTGTGAG GAGTGTGACA AGTGCTTCAG CCAGAGCTCC 
CATCTGAGGC AGCACATGAA GGTGCACAAA GAAAAGAAGC CACATAAGAG GGGCAAAAAT 
GCCAGGGTGA AAACTCACCC GGTGTCCTGG AAAAGGGGCA AAGGGAGGAA GGCGGTGGCT 
GGGATCCGCC AGGTAAAGGG AGCCACTTCC GGCCTTTTTA AAAAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080333.1
CDS	167..880
Translation	MRLGKPKGGISRSASQGKAYESKRKTARQRQKWGVAIRFDSGLSRRRRNVDEKPYKCAKCSKSFSQSSTLFQHKKIHTGKKSHKCADCGKSFFQSSNLIQHRRIHTGEKPYKCDECGERFKQSSNLIQHQRIHTGEKPYCCDECGRCFSQSSHLIQHQRTHTGEKPYQCEECDKCFSQSSHLRQHMKVHKEKKPHKRGKNARVKTHPVSWKRGKGRKAVAGIRQVKGATSGLFKKKK

Target ORF information:

RefSeq Version	NM_026057.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 422 (Zfp422), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026057.3

ORF Insert Sequence:

ATGAGGTTAG GAAAGCCCAA AGGGGGTATT TCTCGAAGCG CCAGCCAAGG AAAAGCCTAT 
GAGAGCAAGC GCAAGACAGC CCGCCAGCGA CAGAAATGGG GGGTGGCCAT TCGCTTCGAC 
TCGGGCTTGA GTCGACGGAG AAGGAACGTG GACGAGAAGC CTTACAAGTG TGCTAAATGC 
TCCAAGAGTT TCAGTCAGAG CTCCACGCTC TTTCAGCACA AGAAGATCCA TACTGGGAAG 
AAATCCCATA AATGTGCCGA TTGTGGGAAA AGTTTCTTTC AGAGTTCTAA CCTCATCCAG 
CACCGGCGGA TCCACACGGG GGAAAAGCCC TATAAGTGTG ACGAGTGTGG AGAGAGATTC 
AAGCAGAGCT CCAATCTCAT CCAGCACCAG AGGATCCACA CCGGCGAGAA GCCCTACTGC 
TGTGATGAGT GTGGCCGGTG CTTTAGCCAG AGTTCCCACC TCATTCAGCA CCAGAGAACC 
CACACCGGAG AGAAGCCCTA CCAGTGTGAG GAGTGTGACA AGTGCTTCAG CCAGAGCTCC 
CATCTGAGGC AGCACATGAA GGTGCACAAA GAAAAGAAGC CACATAAGAG GGGCAAAAAT 
GCCAGGGTGA AAACTCACCC GGTGTCCTGG AAAAGGGGCA AAGGGAGGAA GGCGGTGGCT 
GGGATCCGCC AGGTAAAGGG AGCCACTTCC GGCCTTTTTA AAAAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Zfp422 ( XM_030255555.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030111415.1 Mus musculus zinc finger protein 422 (Zfp422), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu14970
Clone ID Related Accession (Same CDS sequence)	NM_001302439.1 , NM_001302440.1 , NM_026057.3 , XM_030255555.1
Accession Version	XM_030255555.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	zinc finger protein 22 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000072.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGTTAG GAAAGCCCAA AGGGGGTATT TCTCGAAGCG CCAGCCAAGG AAAAGCCTAT 
GAGAGCAAGC GCAAGACAGC CCGCCAGCGA CAGAAATGGG GGGTGGCCAT TCGCTTCGAC 
TCGGGCTTGA GTCGACGGAG AAGGAACGTG GACGAGAAGC CTTACAAGTG TGCTAAATGC 
TCCAAGAGTT TCAGTCAGAG CTCCACGCTC TTTCAGCACA AGAAGATCCA TACTGGGAAG 
AAATCCCATA AATGTGCCGA TTGTGGGAAA AGTTTCTTTC AGAGTTCTAA CCTCATCCAG 
CACCGGCGGA TCCACACGGG GGAAAAGCCC TATAAGTGTG ACGAGTGTGG AGAGAGATTC 
AAGCAGAGCT CCAATCTCAT CCAGCACCAG AGGATCCACA CCGGCGAGAA GCCCTACTGC 
TGTGATGAGT GTGGCCGGTG CTTTAGCCAG AGTTCCCACC TCATTCAGCA CCAGAGAACC 
CACACCGGAG AGAAGCCCTA CCAGTGTGAG GAGTGTGACA AGTGCTTCAG CCAGAGCTCC 
CATCTGAGGC AGCACATGAA GGTGCACAAA GAAAAGAAGC CACATAAGAG GGGCAAAAAT 
GCCAGGGTGA AAACTCACCC GGTGTCCTGG AAAAGGGGCA AAGGGAGGAA GGCGGTGGCT 
GGGATCCGCC AGGTAAAGGG AGCCACTTCC GGCCTTTTTA AAAAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030111415.1
CDS	294..1007
Translation	MRLGKPKGGISRSASQGKAYESKRKTARQRQKWGVAIRFDSGLSRRRRNVDEKPYKCAKCSKSFSQSSTLFQHKKIHTGKKSHKCADCGKSFFQSSNLIQHRRIHTGEKPYKCDECGERFKQSSNLIQHQRIHTGEKPYCCDECGRCFSQSSHLIQHQRTHTGEKPYQCEECDKCFSQSSHLRQHMKVHKEKKPHKRGKNARVKTHPVSWKRGKGRKAVAGIRQVKGATSGLFKKKK

Target ORF information:

RefSeq Version	XM_030255555.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus zinc finger protein 422 (Zfp422), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030255555.1

ORF Insert Sequence:

ATGAGGTTAG GAAAGCCCAA AGGGGGTATT TCTCGAAGCG CCAGCCAAGG AAAAGCCTAT 
GAGAGCAAGC GCAAGACAGC CCGCCAGCGA CAGAAATGGG GGGTGGCCAT TCGCTTCGAC 
TCGGGCTTGA GTCGACGGAG AAGGAACGTG GACGAGAAGC CTTACAAGTG TGCTAAATGC 
TCCAAGAGTT TCAGTCAGAG CTCCACGCTC TTTCAGCACA AGAAGATCCA TACTGGGAAG 
AAATCCCATA AATGTGCCGA TTGTGGGAAA AGTTTCTTTC AGAGTTCTAA CCTCATCCAG 
CACCGGCGGA TCCACACGGG GGAAAAGCCC TATAAGTGTG ACGAGTGTGG AGAGAGATTC 
AAGCAGAGCT CCAATCTCAT CCAGCACCAG AGGATCCACA CCGGCGAGAA GCCCTACTGC 
TGTGATGAGT GTGGCCGGTG CTTTAGCCAG AGTTCCCACC TCATTCAGCA CCAGAGAACC 
CACACCGGAG AGAAGCCCTA CCAGTGTGAG GAGTGTGACA AGTGCTTCAG CCAGAGCTCC 
CATCTGAGGC AGCACATGAA GGTGCACAAA GAAAAGAAGC CACATAAGAG GGGCAAAAAT 
GCCAGGGTGA AAACTCACCC GGTGTCCTGG AAAAGGGGCA AAGGGAGGAA GGCGGTGGCT 
GGGATCCGCC AGGTAAAGGG AGCCACTTCC GGCCTTTTTA AAAAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

Tbx22 expressions during palatal development in fetuses with glucocorticoid-/alcohol-induced C57BL/6N cleft palates.
The Journal of craniofacial surgery20(5)1316-26(2009 Sep)
Kim SM,Lee JH,Jabaiti S,Lee SK,Choi JY

Molecular cloning, chromosomal mapping, and characteristic expression in tooth organ of rat and mouse Krox-25.
Genomics83(2)243-53(2004 Feb)
Lee SK,Kim YS,Lee SS,Lee YJ,Song IS,Park SC,Kozak C,Yamada Y

The human KROX-26/ZNF22 gene is expressed at sites of tooth formation and maps to the locus for permanent tooth agenesis (He-Zhao deficiency).
Journal of dental research82(12)1002-7(2003 Dec)
Gao Y,Kobayashi H,Ganss B

Developmental expression pattern and DNA-binding properties of the zinc finger transcription factor Krox-26.
Connective tissue research44 Suppl 1161-6(2003)
Teo W,Chen H,Poon T,Ganss B

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Zfp422 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

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