Xylt1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Xylt1
Entrez Gene ID	233781
Full Name	xylosyltransferase 1
Synonyms	8030490L12
General protein information	Preferred Names xylosyltransferase 1 Names xylosyltransferase 1 peptide O-xylosyltransferase 1 xylosyltransferase I
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_175645.3	NP_783576.2	xylosyltransferase 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	XYLT1	NP_001032366.1
Bos taurus (cattle)	XYLT1	XP_002698083.1
Mus musculus (house mouse)	Xylt1	NP_783576.2
Gallus gallus (chicken)	XYLT1	XP_414904.3
Drosophila melanogaster (fruit fly)	oxt	NP_647705.1
Macaca mulatta (Rhesus monkey)	LOC718187	XP_001110525.2
Xenopus tropicalis (tropical clawed frog)	xylt1	NP_001120412.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005811	XP_556482.2
Homo sapiens (human)	XYLT1	NP_071449.1
Canis lupus familiaris (dog)	XYLT1	NP_001008718.1
Rattus norvegicus (Norway rat)	Xylt1	XP_006230213.1
Danio rerio (zebrafish)	xylt1	NP_001165868.1

Gene Ontology
Bibliography

Related articles in PubMed

Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism.
Yu H, Takeuchi M, LeBarron J, Kantharia J, London E, Bakker H, Haltiwanger RS, Li H, Takeuchi H
Nature chemical biology11(11)847-54(2015 Nov)

Forward genetics defines Xylt1 as a key, conserved regulator of early chondrocyte maturation and skeletal length.
Mis EK, Liem KF Jr, Kong Y, Schwartz NB, Domowicz M, Weatherbee SD
Developmental biology385(1)67-82(2014 Jan)

Mouse Kif7/Costal2 is a cilia-associated protein that regulates Sonic hedgehog signaling.
Liem KF Jr, He M, Ocbina PJ, Anderson KV
Proceedings of the National Academy of Sciences of the United States of America106(32)13377-82(2009 Aug)

Analysis of HSF4 binding regions reveals its necessity for gene regulation during development and heat shock response in mouse lenses.
Fujimoto M, Oshima K, Shinkawa T, Wang BB, Inouye S, Hayashida N, Takii R, Nakai A
The Journal of biological chemistry283(44)29961-70(2008 Oct)

Biosynthesis of chondroitin and heparan sulfate in chinese hamster ovary cells depends on xylosyltransferase II.
Cuellar K, Chuong H, Hubbell SM, Hinsdale ME
The Journal of biological chemistry282(8)5195-200(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Xylt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Xylt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu20788	NM_175645.3 Latest version!	Mus musculus xylosyltransferase 1 (Xylt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Xylt1 ( NM_175645.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_783576.2 Mus musculus xylosyltransferase 1 (Xylt1), mRNA.

Price & Availability↑

CloneID	OMu20788
Clone ID Related Accession (Same CDS sequence)	NM_175645.3
Accession Version	NM_175645.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568995200000
Organism	Mus musculus(house mouse)
Product	xylosyltransferase 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK033309.1, CJ128023.1, AJ291750.1, AC102076.9, BM964190.1, BI990840.1 and AC122836.4. On Dec 29, 2006 this sequence version replaced NM_175645.2. ##Evidence-Data-START## Transcript exon combination :: AJ539164.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGTGGCGG CGCCGTGCGC CCGGAGGCTG GCGCGGCGCT CGCACTCGGC GCTGCTGGCG 
GCGCTCATGG TGCTGCTGCT GCAGACGCTG GTGGTGTGGA ATTTCAGCAG CCTGGACTCC 
GGGGCTGGAG AGCAGCGGCG CGCGGGGGCA GCGGCGGGAG CAGCCGAGCA GCAGCAGCCC 
GCGGCCCCGC GCCGGGAGCG CAGGGACCTG GCTGCCCATC TGCCCGCAGC CCGCGGAGGA 
CCTGGAGGCC GAGCCGGCGG AGGAGGAGCA CGGGGAGGCG GCCCCGGAGG AGCCCGCGCA 
CAGCAGCCTG CCAGCCGCGG GGCACTAGCC TCCCGGGCGA GGGATCCACA ACCAAGTCCA 
CTGATCACCC TAGAGACCCA GGATGGCTAC TTCTCTCACC GTCCCAAAGA GAAAGTTCGG 
ACCGACAGCA ACAATGAGAA CTCAGTCCCC AAGGACTTTG AGAATGTGGA TAACAGCAAC 
TTCGCCCCCA GGACCCAGAA GCAGAAACAC CAGCCAGAGC TGGCAAAGAA GCCCCCCAGC 
AGGCAGAAAG AGCATTTGCA GAGAAAGCTG GATGCCCAGG ACAAACGACA GGGCCAGTCA 
GTCCTGGGGA AAGGCCCCAA GGAGGTGCTG CCTCCTCGGG AGAAAGCCAC AGGCAACAGT 
AGCCAAGGGA AGGATCTCTC AAGACACAGC CATGCCAGGA AGAGTGGTGG TGGTGGGTCC 
CCCGAAACCA AGTCTGACCA GGCCCCCAAG TGTGATATCT CTGGCAAGGA GGCCATCTCA 
GCACTGACCC GCGCTAAGTC CAAGCATTGT CGCCAGGAGA TTGCAGAAAC CTACTGTCGC 
CACAAGCTGG GGCTGCTGAT GCCAGAGAAG GTGGCTCGAT TCTGTCCCCT AGAAGGCAAA 
GCCAACAAGA ATGTCCAGTG GGATGAGGAT GCTGTGGAGT ACATGCCTGC CAACCCAGTC 
AGAATCGCCT TTGTCCTGGT GGTCCATGGC CGTGCCTCTC GACAGCTACA GCGCATGTTC 
AAGGCCATCT ACCACAAAGA CCATTTCTAC TATATCCATG TGGATAAGCG TTCCAATTAC 
CTGCATCGGC AAGTGCTCCA GTTCTCCAGG CAGTACGACA ATGTCCGAGT CACCTCCTGG 
AGGATGGCCA CCATTTGGGG TGGAGCCAGC CTCCTGTCCA CCTACCTGCA GAGCATGCGG 
GATCTACTAG AGATGACTGA CTGGCCCTGG GACTTCTTCA TCAACCTCAG TGCTGCTGAC 
TACCCCATCA GGACAAATGA CCAGCTGGTA GCATTTCTTT CCAGATATCG AGATATGAAC 
TTCCTGAAGT CACATGGCCG GGACAATGCA AGGTTCATCC GGAAGCAGGG CCTGGACCGC 
CTCTTCCTGG AGTGTGATAC ACACATGTGG CGCCTGGGGG ACCGGCGGAT CCCAGAGGGC 
ATTGCTGTGG ATGGTGGTTC TGATTGGTTC CTGCTAAACA GGAAGTTTGT AGAGTATGTG 
GCATTCTCCA CAGATGACCT GGTGACCAAG ATGAAGCAGT TCTACTCTTA CACCCTTCTC 
CCTGCTGAGT CCTTTTTCCA CACGGTTCTA GAGAACAGCC CCCACTGTGA CACCATGGTG 
GATAACAACC TGCGCATCAC CAACTGGAAC CGCAAGCTGG GCTGCAAGTG CCAGTACAAG 
CATATCGTGG ACTGGTGCGG CTGCTCTCCC AATGACTTCA AGCCTCAGGA CTTCCATCGC 
TTCCAGCAGA CAGCCCGGCC CACCTTCTTT GCCCGAAAGT TCGAAGCCAT AGTGAACCAG 
GAGATCATTG GGCAGCTGGA CTCTTACCTG TACGGGAACT ATCCTGCGGG CACCCCGGGC 
CTCCGCTCCT ACTGGGAGAA TGTCTACGAT GAACCAGATG GCATCCACAC CCTCAGCGAT 
GTAGCCCTCA CCCTGTACCA TTCCTTCATC CGTCTGGGTC TTCGAAGAGC TGAGTCATCC 
CTACACACGG ATGGGGAGAA CAGCTGCAGG TACTACCCAA TGGGCCACCC AGCTTCTGTC 
CATCTCTACT TCCTTGCTGA CCGATTCCAG GGCTTTCTGA TCAAGCATCA CGTGACCAAC 
CTTGCTGTGA GCAAACTGGA GACACTGGAG ACATGGATGA TGCCAAAGAA AGTCTTCAAG 
GTCGCAAGTC CCCCCAGTGA CTTTGGAAGG CTTCAGTTTT CTGAGGTTGG CACTGACTGG 
GATGCCAAGG AGAGGCTGTT CCGGAACTTT GGTGGTCTTT TGGGGCCCAT GGATGAGCCG 
GTGGGGATGC AGAAATGGGG AAAGGGGCCC AATGTGACCG TGACTGTTAT TTGGGTGGAT 
CCTGTCAACG TCATTGCAGC CACCTATGAT ATCCTGATTG AGTCCACTGC GGAATTCACA 
CACTACAAGC CCCCTTTGAA TCTGCCTCTG AGGCCTGGGG TCTGGACAGT GAAGATTCTC 
CATCACTGGG TGCCAGTGGC AGAGACCAAA TTCCTTGTGG CACCTTTGAC CTTTTCCAAC 
AAGCAGCCCA TCAAACCAGA GGAGGCTTTG AAGCTGCACA ATGGGCCACC TCGCAGTGCC 
TACATGGAGC AGAGTTTCCA GAGCCTGAAC CCAGTCCTCA GCCTGCACAT CAATCCTGCC 
CAAGTCGAAC AGGCCCGGAA GAATGCAGCC TTCACCGGGA CAGCGCTAGA AGCCTGGCTG 
GACTCGCTGG TGGGTGGGAC TTGGACTGCC ATGGACATCT GCACCACAGG CCCCACCGCC 
TGCCCAGTCA TGCAGACCTG CAGCCAAACA GCCTGGAGTT CCTTCAGCCC TGATCCCAAG 
TCAGAGCTGG GTGCAGTCAA ACCTGACGGA CGGCTCAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_783576.2
CDS	201..3062
Translation	MVAAPCARRLARRSHSALLAALMVLLLQTLVVWNFSSLDSGAGEQRRAGAAAGAAEQQQPAAPRRERRDLAAHLPAARGGPGGRAGGGGARGGGPGGARAQQPASRGALASRARDPQPSPLITLETQDGYFSHRPKEKVRTDSNNENSVPKDFENVDNSNFAPRTQKQKHQPELAKKPPSRQKEHLQRKLDAQDKRQGQSVLGKGPKEVLPPREKATGNSSQGKDLSRHSHARKSGGGGSPETKSDQAPKCDISGKEAISALTRAKSKHCRQEIAETYCRHKLGLLMPEKVARFCPLEGKANKNVQWDEDAVEYMPANPVRIAFVLVVHGRASRQLQRMFKAIYHKDHFYYIHVDKRSNYLHRQVLQFSRQYDNVRVTSWRMATIWGGASLLSTYLQSMRDLLEMTDWPWDFFINLSAADYPIRTNDQLVAFLSRYRDMNFLKSHGRDNARFIRKQGLDRLFLECDTHMWRLGDRRIPEGIAVDGGSDWFLLNRKFVEYVAFSTDDLVTKMKQFYSYTLLPAESFFHTVLENSPHCDTMVDNNLRITNWNRKLGCKCQYKHIVDWCGCSPNDFKPQDFHRFQQTARPTFFARKFEAIVNQEIIGQLDSYLYGNYPAGTPGLRSYWENVYDEPDGIHTLSDVALTLYHSFIRLGLRRAESSLHTDGENSCRYYPMGHPASVHLYFLADRFQGFLIKHHVTNLAVSKLETLETWMMPKKVFKVASPPSDFGRLQFSEVGTDWDAKERLFRNFGGLLGPMDEPVGMQKWGKGPNVTVTVIWVDPVNVIAATYDILIESTAEFTHYKPPLNLPLRPGVWTVKILHHWVPVAETKFLVAPLTFSNKQPIKPEEALKLHNGPPRSAYMEQSFQSLNPVLSLHINPAQVEQARKNAAFTGTALEAWLDSLVGGTWTAMDICTTGPTACPVMQTCSQTAWSSFSPDPKSELGAVKPDGRLR

Target ORF information:

RefSeq Version	NM_175645.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus xylosyltransferase 1 (Xylt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175645.3

ORF Insert Sequence:

ATGGTGGCGG CGCCGTGCGC CCGGAGGCTG GCGCGGCGCT CGCACTCGGC GCTGCTGGCG 
GCGCTCATGG TGCTGCTGCT GCAGACGCTG GTGGTGTGGA ATTTCAGCAG CCTGGACTCC 
GGGGCTGGAG AGCAGCGGCG CGCGGGGGCA GCGGCGGGAG CAGCCGAGCA GCAGCAGCCC 
GCGGCCCCGC GCCGGGAGCG CAGGGACCTG GCTGCCCATC TGCCCGCAGC CCGCGGAGGA 
CCTGGAGGCC GAGCCGGCGG AGGAGGAGCA CGGGGAGGCG GCCCCGGAGG AGCCCGCGCA 
CAGCAGCCTG CCAGCCGCGG GGCACTAGCC TCCCGGGCGA GGGATCCACA ACCAAGTCCA 
CTGATCACCC TAGAGACCCA GGATGGCTAC TTCTCTCACC GTCCCAAAGA GAAAGTTCGG 
ACCGACAGCA ACAATGAGAA CTCAGTCCCC AAGGACTTTG AGAATGTGGA TAACAGCAAC 
TTCGCCCCCA GGACCCAGAA GCAGAAACAC CAGCCAGAGC TGGCAAAGAA GCCCCCCAGC 
AGGCAGAAAG AGCATTTGCA GAGAAAGCTG GATGCCCAGG ACAAACGACA GGGCCAGTCA 
GTCCTGGGGA AAGGCCCCAA GGAGGTGCTG CCTCCTCGGG AGAAAGCCAC AGGCAACAGT 
AGCCAAGGGA AGGATCTCTC AAGACACAGC CATGCCAGGA AGAGTGGTGG TGGTGGGTCC 
CCCGAAACCA AGTCTGACCA GGCCCCCAAG TGTGATATCT CTGGCAAGGA GGCCATCTCA 
GCACTGACCC GCGCTAAGTC CAAGCATTGT CGCCAGGAGA TTGCAGAAAC CTACTGTCGC 
CACAAGCTGG GGCTGCTGAT GCCAGAGAAG GTGGCTCGAT TCTGTCCCCT AGAAGGCAAA 
GCCAACAAGA ATGTCCAGTG GGATGAGGAT GCTGTGGAGT ACATGCCTGC CAACCCAGTC 
AGAATCGCCT TTGTCCTGGT GGTCCATGGC CGTGCCTCTC GACAGCTACA GCGCATGTTC 
AAGGCCATCT ACCACAAAGA CCATTTCTAC TATATCCATG TGGATAAGCG TTCCAATTAC 
CTGCATCGGC AAGTGCTCCA GTTCTCCAGG CAGTACGACA ATGTCCGAGT CACCTCCTGG 
AGGATGGCCA CCATTTGGGG TGGAGCCAGC CTCCTGTCCA CCTACCTGCA GAGCATGCGG 
GATCTACTAG AGATGACTGA CTGGCCCTGG GACTTCTTCA TCAACCTCAG TGCTGCTGAC 
TACCCCATCA GGACAAATGA CCAGCTGGTA GCATTTCTTT CCAGATATCG AGATATGAAC 
TTCCTGAAGT CACATGGCCG GGACAATGCA AGGTTCATCC GGAAGCAGGG CCTGGACCGC 
CTCTTCCTGG AGTGTGATAC ACACATGTGG CGCCTGGGGG ACCGGCGGAT CCCAGAGGGC 
ATTGCTGTGG ATGGTGGTTC TGATTGGTTC CTGCTAAACA GGAAGTTTGT AGAGTATGTG 
GCATTCTCCA CAGATGACCT GGTGACCAAG ATGAAGCAGT TCTACTCTTA CACCCTTCTC 
CCTGCTGAGT CCTTTTTCCA CACGGTTCTA GAGAACAGCC CCCACTGTGA CACCATGGTG 
GATAACAACC TGCGCATCAC CAACTGGAAC CGCAAGCTGG GCTGCAAGTG CCAGTACAAG 
CATATCGTGG ACTGGTGCGG CTGCTCTCCC AATGACTTCA AGCCTCAGGA CTTCCATCGC 
TTCCAGCAGA CAGCCCGGCC CACCTTCTTT GCCCGAAAGT TCGAAGCCAT AGTGAACCAG 
GAGATCATTG GGCAGCTGGA CTCTTACCTG TACGGGAACT ATCCTGCGGG CACCCCGGGC 
CTCCGCTCCT ACTGGGAGAA TGTCTACGAT GAACCAGATG GCATCCACAC CCTCAGCGAT 
GTAGCCCTCA CCCTGTACCA TTCCTTCATC CGTCTGGGTC TTCGAAGAGC TGAGTCATCC 
CTACACACGG ATGGGGAGAA CAGCTGCAGG TACTACCCAA TGGGCCACCC AGCTTCTGTC 
CATCTCTACT TCCTTGCTGA CCGATTCCAG GGCTTTCTGA TCAAGCATCA CGTGACCAAC 
CTTGCTGTGA GCAAACTGGA GACACTGGAG ACATGGATGA TGCCAAAGAA AGTCTTCAAG 
GTCGCAAGTC CCCCCAGTGA CTTTGGAAGG CTTCAGTTTT CTGAGGTTGG CACTGACTGG 
GATGCCAAGG AGAGGCTGTT CCGGAACTTT GGTGGTCTTT TGGGGCCCAT GGATGAGCCG 
GTGGGGATGC AGAAATGGGG AAAGGGGCCC AATGTGACCG TGACTGTTAT TTGGGTGGAT 
CCTGTCAACG TCATTGCAGC CACCTATGAT ATCCTGATTG AGTCCACTGC GGAATTCACA 
CACTACAAGC CCCCTTTGAA TCTGCCTCTG AGGCCTGGGG TCTGGACAGT GAAGATTCTC 
CATCACTGGG TGCCAGTGGC AGAGACCAAA TTCCTTGTGG CACCTTTGAC CTTTTCCAAC 
AAGCAGCCCA TCAAACCAGA GGAGGCTTTG AAGCTGCACA ATGGGCCACC TCGCAGTGCC 
TACATGGAGC AGAGTTTCCA GAGCCTGAAC CCAGTCCTCA GCCTGCACAT CAATCCTGCC 
CAAGTCGAAC AGGCCCGGAA GAATGCAGCC TTCACCGGGA CAGCGCTAGA AGCCTGGCTG 
GACTCGCTGG TGGGTGGGAC TTGGACTGCC ATGGACATCT GCACCACAGG CCCCACCGCC 
TGCCCAGTCA TGCAGACCTG CAGCCAAACA GCCTGGAGTT CCTTCAGCCC TGATCCCAAG 
TCAGAGCTGG GTGCAGTCAA ACCTGACGGA CGGCTCAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism.
Nature chemical biology11(11)847-54(2015 Nov)
Yu H,Takeuchi M,LeBarron J,Kantharia J,London E,Bakker H,Haltiwanger RS,Li H,Takeuchi H

Forward genetics defines Xylt1 as a key, conserved regulator of early chondrocyte maturation and skeletal length.
Developmental biology385(1)67-82(2014 Jan)
Mis EK,Liem KF Jr,Kong Y,Schwartz NB,Domowicz M,Weatherbee SD

Mouse Kif7/Costal2 is a cilia-associated protein that regulates Sonic hedgehog signaling.
Proceedings of the National Academy of Sciences of the United States of America106(32)13377-82(2009 Aug)
Liem KF Jr,He M,Ocbina PJ,Anderson KV

Analysis of HSF4 binding regions reveals its necessity for gene regulation during development and heat shock response in mouse lenses.
The Journal of biological chemistry283(44)29961-70(2008 Oct)
Fujimoto M,Oshima K,Shinkawa T,Wang BB,Inouye S,Hayashida N,Takii R,Nakai A

Biosynthesis of chondroitin and heparan sulfate in chinese hamster ovary cells depends on xylosyltransferase II.
The Journal of biological chemistry282(8)5195-200(2007 Feb)
Cuellar K,Chuong H,Hubbell SM,Hinsdale ME

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Xylt1 cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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