Fbxo21 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fbxo21
Entrez Gene ID	231670
Full Name	F-box protein 21
Synonyms	2810425J22Rik,AU016673
General protein information	Preferred Names F-box protein 21 Names F-box only protein 21
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 F

mRNA and Protein(s)

mRNA	Protein	Name
NM_001346588.1	NP_001333517.1	F-box only protein 21 isoform 2
NM_145564.4	NP_663539.1	F-box only protein 21 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	FBXO21	NP_296373.1
Pan troglodytes (chimpanzee)	FBXO21	XP_001155956.1
Mus musculus (house mouse)	Fbxo21	NP_663539.1
Danio rerio (zebrafish)	fbxo21	XP_696130.3
Xenopus tropicalis (tropical clawed frog)	fbxo21	NP_001120516.1
Macaca mulatta (Rhesus monkey)	FBXO21	XP_001083109.1
Canis lupus familiaris (dog)	FBXO21	XP_003433428.1
Gallus gallus (chicken)	FBXO21	XP_425297.2
Rattus norvegicus (Norway rat)	Fbxo21	NP_001101808.2

Gene Ontology
Bibliography

Related articles in PubMed

Lys29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response.
Yu Z, Chen T, Li X, Yang M, Tang S, Zhu X, Gu Y, Su X, Xia M, Li W, Zhang X, Wang Q, Cao X, Wang J
eLife5(2016 04)

Systematic analysis and nomenclature of mammalian F-box proteins.
Jin J, Cardozo T, Lovering RC, Elledge SJ, Pagano M, Harper JW
Genes & development18(21)2573-80(2004 Nov)

Construction of a multi-functional cDNA library specific for mouse pancreatic islets and its application to microarray.
Nishimura M, Yokoi N, Miki T, Horikawa Y, Yoshioka H, Takeda J, Ohara O, Seino S
DNA research : an international journal for rapid publication of reports on genes and genomes11(5)315-23(2004 Oct)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
Okazaki N, Kikuno R, Ohara R, Inamoto S, Hara Y, Nagase T, Ohara O, Koga H
DNA research : an international journal for rapid publication of reports on genes and genomes9(5)179-88(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Fbxo21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fbxo21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11793	NM_145564.4 Latest version!	Mus musculus F-box protein 21 (Fbxo21), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu35874	NM_001346588.1 Latest version!	Mus musculus F-box protein 21 (Fbxo21), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fbxo21 ( NM_145564.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_663539.1 Mus musculus F-box protein 21 (Fbxo21), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu11793
Clone ID Related Accession (Same CDS sequence)	NM_145564.4
Accession Version	NM_145564.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571241600000
Organism	Mus musculus(house mouse)
Product	F-box only protein 21 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK049723.1 and BP754360.1. On Oct 21, 2016 this sequence version replaced NM_145564.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK049723.1, SRR1660815.205993.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCGG TAGCGGGGGA CAGCGCTATG GAGGTGGTGC CGGCGCTGGC CGAGGAGGCC 
GCGGCTGAGG CGACGGGCCC CAGCTGCCTG GTGCAGCTGC CGGGCGAGGT GCTGGAGTAC 
ATCCTGTGCA GCGGCTCGCT CACGGCGCTG GACATCGGCC GCGTGTCCAG CACCTGCCGC 
CGCCTGCGCG AGGTGTGCCA GAGCAGCGGG CAGGTGTGGA AGGAGCAGTT CCGGGTGAGG 
TGGCCCTCCC TGATGAAGCA CTACAGCCCC ACCGACTACG TCAATTGGTT AGAGGAATAT 
AAAGTTCGGC AGAAAGCTGG GTTAGAAGCC CGGAAGATTG TAGCTTCCTT CTCCAAGCGG 
TTCTTTTCAG AGCACGTCCC CTGTAATGGC TTCAGTGACA TTGAAAACCT TGAGGGCCCA 
GAGATCTTTT TTGAGGATGA GCTGGTGTGT ATCCTAAACA TGGAAGGAAG GAAAGCTTTG 
ACCTGGAAAT ACTACGCAAA GAAAATTCTC TACTACCTGC GGCAGCAGAA AATCCTCAAT 
AACCTCAAGG CGTTCCTGCA GCAGCCCGAC GATTACGAGT CCTATCTCGA AGGTGCCGTT 
TACATCGACC AGTACTGCAA CCCACTCTCC GACATCAGCT TCAGAGACAT CCAGGCCCAG 
ATCCACAGCA TTGTGGAGCT TGTGTGCAAA ACCCTCCGTG GCATCAACAG CCGCCACCCC 
AGCCTGACCT TCAGGGCAGG TGAGTCCTCT ATGATAATGG AGATAGAACT TCAGAGCCAA 
GTGCTGGACG CTATCAACTA TGTCCTGTAT GACCAGCTGA AGTTCAAAGG GAACCGCATG 
GACTACTATA ATGCCTTGAA CCTCTACATG CACCAGGTGT TGACCCGCAG AACAGGAATC 
CCCATCAGCA TGTCTCTGCT CTACCTGACC GTCGCCCGGC AGCTGGGGGT TCCCCTGGAG 
CCTGTCAACT TCCCAAGCCA CTTCCTGCTG CGGTGGTGCC AAGGTGCAGA AGGGGCAACC 
CTGGACATCT TTGACTACAT CTACATAGAT GCTTTTGGGA AGGGCAAGCA GCTGACAGTG 
AAAGAGTGTG AGTACCTGAT TGGCCAGCAC GTGACGGCAG CACTCTACGG CGTGGTGAAT 
GTGAAGAAGG TGCTACAGCG GATGGTGGGC AACTTGCTGA GCCTGGGCAA GAGGGAAGGC 
ATTGACCAGT CCTACCAGCT CCTCAGAGAC TCCTTGGACC TGTACCTGGC GATGTACCCG 
GACCAGGTGC AGCTCCTGCT CCTCCAAGCC AGACTCTACT TCCACCTGGG CATCTGGCCA 
GAGAAGTCTT TCTGTCTTGT CTTGAAGGTG CTTGACATCC TCCAGCACAT CCAGACCCTG 
GACCCCGGGC AGCACGGGGC GGTGGGCTAT CTCGTGCAGC ATACCTTAGA GCACATTGAG 
CGTAAGAAGG AGGAGGTGGG CGTGGAGGTG AAGCTGCGCT CCGAGGAGAA GCACAGGGAT 
GTCTGCTACT CCATCGGGCT TGTGATGAAG CATAAGAGGT ACGGCTACAA CTGTGTGATC 
TACGGCTGGG ACCCTACGTG CATGATGGGG CATGAGTGGA TCCGAAACAT GAATGTGCAC 
AGCCTGCCTC ATGGCCACCA TCAGCCCTTC TACAACGTCC TGGTGGAGGA TGGCTCCTGC 
AGATATGCAG CCCAAGAAAA CCTGGAATAC AACGTGGAGC CTCAGGAAAT CTCGCACCCA 
GATGTGGGGC GCTACTTCTC GGAGTTCACA GGCACACACT ACATCCCAAA TGCAGAGCTG 
GAGATCCGGT ACCCCGAGGA CCTGGAGTTT GTGTACGAGA CAGTGCAGAA TATTTACAGT 
GCAAAGGAGG ACACAGCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663539.1
CDS	32..1915
Translation	MASVAGDSAMEVVPALAEEAAAEATGPSCLVQLPGEVLEYILCSGSLTALDIGRVSSTCRRLREVCQSSGQVWKEQFRVRWPSLMKHYSPTDYVNWLEEYKVRQKAGLEARKIVASFSKRFFSEHVPCNGFSDIENLEGPEIFFEDELVCILNMEGRKALTWKYYAKKILYYLRQQKILNNLKAFLQQPDDYESYLEGAVYIDQYCNPLSDISFRDIQAQIHSIVELVCKTLRGINSRHPSLTFRAGESSMIMEIELQSQVLDAINYVLYDQLKFKGNRMDYYNALNLYMHQVLTRRTGIPISMSLLYLTVARQLGVPLEPVNFPSHFLLRWCQGAEGATLDIFDYIYIDAFGKGKQLTVKECEYLIGQHVTAALYGVVNVKKVLQRMVGNLLSLGKREGIDQSYQLLRDSLDLYLAMYPDQVQLLLLQARLYFHLGIWPEKSFCLVLKVLDILQHIQTLDPGQHGAVGYLVQHTLEHIERKKEEVGVEVKLRSEEKHRDVCYSIGLVMKHKRYGYNCVIYGWDPTCMMGHEWIRNMNVHSLPHGHHQPFYNVLVEDGSCRYAAQENLEYNVEPQEISHPDVGRYFSEFTGTHYIPNAELEIRYPEDLEFVYETVQNIYSAKEDTAE

Target ORF information:

RefSeq Version	NM_145564.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus F-box protein 21 (Fbxo21), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145564.4

ORF Insert Sequence:

ATGGCGTCGG TAGCGGGGGA CAGCGCTATG GAGGTGGTGC CGGCGCTGGC CGAGGAGGCC 
GCGGCTGAGG CGACGGGCCC CAGCTGCCTG GTGCAGCTGC CGGGCGAGGT GCTGGAGTAC 
ATCCTGTGCA GCGGCTCGCT CACGGCGCTG GACATCGGCC GCGTGTCCAG CACCTGCCGC 
CGCCTGCGCG AGGTGTGCCA GAGCAGCGGG CAGGTGTGGA AGGAGCAGTT CCGGGTGAGG 
TGGCCCTCCC TGATGAAGCA CTACAGCCCC ACCGACTACG TCAATTGGTT AGAGGAATAT 
AAAGTTCGGC AGAAAGCTGG GTTAGAAGCC CGGAAGATTG TAGCTTCCTT CTCCAAGCGG 
TTCTTTTCAG AGCACGTCCC CTGTAATGGC TTCAGTGACA TTGAAAACCT TGAGGGCCCA 
GAGATCTTTT TTGAGGATGA GCTGGTGTGT ATCCTAAACA TGGAAGGAAG GAAAGCTTTG 
ACCTGGAAAT ACTACGCAAA GAAAATTCTC TACTACCTGC GGCAGCAGAA AATCCTCAAT 
AACCTCAAGG CGTTCCTGCA GCAGCCCGAC GATTACGAGT CCTATCTCGA AGGTGCCGTT 
TACATCGACC AGTACTGCAA CCCACTCTCC GACATCAGCT TCAGAGACAT CCAGGCCCAG 
ATCCACAGCA TTGTGGAGCT TGTGTGCAAA ACCCTCCGTG GCATCAACAG CCGCCACCCC 
AGCCTGACCT TCAGGGCAGG TGAGTCCTCT ATGATAATGG AGATAGAACT TCAGAGCCAA 
GTGCTGGACG CTATCAACTA TGTCCTGTAT GACCAGCTGA AGTTCAAAGG GAACCGCATG 
GACTACTATA ATGCCTTGAA CCTCTACATG CACCAGGTGT TGACCCGCAG AACAGGAATC 
CCCATCAGCA TGTCTCTGCT CTACCTGACC GTCGCCCGGC AGCTGGGGGT TCCCCTGGAG 
CCTGTCAACT TCCCAAGCCA CTTCCTGCTG CGGTGGTGCC AAGGTGCAGA AGGGGCAACC 
CTGGACATCT TTGACTACAT CTACATAGAT GCTTTTGGGA AGGGCAAGCA GCTGACAGTG 
AAAGAGTGTG AGTACCTGAT TGGCCAGCAC GTGACGGCAG CACTCTACGG CGTGGTGAAT 
GTGAAGAAGG TGCTACAGCG GATGGTGGGC AACTTGCTGA GCCTGGGCAA GAGGGAAGGC 
ATTGACCAGT CCTACCAGCT CCTCAGAGAC TCCTTGGACC TGTACCTGGC GATGTACCCG 
GACCAGGTGC AGCTCCTGCT CCTCCAAGCC AGACTCTACT TCCACCTGGG CATCTGGCCA 
GAGAAGTCTT TCTGTCTTGT CTTGAAGGTG CTTGACATCC TCCAGCACAT CCAGACCCTG 
GACCCCGGGC AGCACGGGGC GGTGGGCTAT CTCGTGCAGC ATACCTTAGA GCACATTGAG 
CGTAAGAAGG AGGAGGTGGG CGTGGAGGTG AAGCTGCGCT CCGAGGAGAA GCACAGGGAT 
GTCTGCTACT CCATCGGGCT TGTGATGAAG CATAAGAGGT ACGGCTACAA CTGTGTGATC 
TACGGCTGGG ACCCTACGTG CATGATGGGG CATGAGTGGA TCCGAAACAT GAATGTGCAC 
AGCCTGCCTC ATGGCCACCA TCAGCCCTTC TACAACGTCC TGGTGGAGGA TGGCTCCTGC 
AGATATGCAG CCCAAGAAAA CCTGGAATAC AACGTGGAGC CTCAGGAAAT CTCGCACCCA 
GATGTGGGGC GCTACTTCTC GGAGTTCACA GGCACACACT ACATCCCAAA TGCAGAGCTG 
GAGATCCGGT ACCCCGAGGA CCTGGAGTTT GTGTACGAGA CAGTGCAGAA TATTTACAGT 
GCAAAGGAGG ACACAGCCGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fbxo21 ( NM_001346588.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001333517.1 Mus musculus F-box protein 21 (Fbxo21), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu35874
Clone ID Related Accession (Same CDS sequence)	NM_001346588.1
Accession Version	NM_001346588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571241600000
Organism	Mus musculus(house mouse)
Product	F-box only protein 21 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK049723.1, AK048131.1, BY478760.1, BE954194.1 and BP754360.1. On Oct 21, 2016 this sequence version replaced XM_006530297.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. It encodes isoform 2, which lacks an internal in-frame segment and is shorter, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AB093270.1, AK048131.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGTCGG TAGCGGGGGA CAGCGCTATG GAGGTGGTGC CGGCGCTGGC CGAGGAGGCC 
GCGGCTGAGG CGACGGGCCC CAGCTGCCTG GTGCAGCTGC CGGGCGAGGT GCTGGAGTAC 
ATCCTGTGCA GCGGCTCGCT CACGGCGCTG GACATCGGCC GCGTGTCCAG CACCTGCCGC 
CGCCTGCGCG AGGTGTGCCA GAGCAGCGGG CAGGTGTGGA AGGAGCAGTT CCGGGTGAGG 
TGGCCCTCCC TGATGAAGCA CTACAGCCCC ACCGACTACG TCAATTGGTT AGAGGAATAT 
AAAGTTCGGC AGAAAGCTGG GTTAGAAGCC CGGAAGATTG TAGCTTCCTT CTCCAAGCGG 
TTCTTTTCAG AGCACGTCCC CTGTAATGGC TTCAGTGACA TTGAAAACCT TGAGGGCCCA 
GAGATCTTTT TTGAGGATGA GCTGGTGTGT ATCCTAAACA TGGAAGGAAG GAAAGCTTTG 
ACCTGGAAAT ACTACGCAAA GAAAATTCTC TACTACCTGC GGCAGCAGAA AATCCTCAAT 
AACCTCAAGG CGTTCCTGCA GCAGCCCGAC GATTACGAGT CCTATCTCGA AGGTGCCGTT 
TACATCGACC AGTACTGCAA CCCACTCTCC GACATCAGCT TCAGAGACAT CCAGGCCCAG 
ATCCACAGCA TTGTGGAGCT TGTGTGCAAA ACCCTCCGTG GCATCAACAG CCGCCACCCC 
AGCCTGACCT TCAGGGCAGG TGAGTCCTCT ATGATAATGG AGATAGAACT TCAGAGCCAA 
GTGCTGGACG CTATCAACTA TGTCCTGTAT GACCAGCTGA AGTTCAAAGG GAACCGCATG 
GACTACTATA ATGCCTTGAA CCTCTACATG CACCAGGTGT TGACCCGCAG AACAGGAATC 
CCCATCAGCA TGTCTCTGCT CTACCTGACC GTCGCCCGGC AGCTGGGGGT TCCCCTGGAG 
CCTGTCAACT TCCCAAGCCA CTTCCTGCTG CGGTGGTGCC AAGGTGCAGA AGGGGCAACC 
CTGGACATCT TTGACTACAT CTACATAGAT GCTTTTGGGA AGGGCAAGCA GCTGACAGTG 
AAAGAGTGTG AGTACCTGAT TGGCCAGCAC GTGACGGCAG CACTCTACGG CGTGGTGAAT 
GTGAAGAAGG TGCTACAGCG GATGGTGGGC AACTTGCTGA GCCTGGGCAA GAGGGAAGGC 
ATTGACCAGT CCTACCAGCT CCTCAGAGAC TCCTTGGACC TGTACCTGGC GATGTACCCG 
GACCAGGTGC AGCTCCTGCT CCTCCAAGCC AGACTCTACT TCCACCTGGG CATCTGGCCA 
GAGAAGGTGC TTGACATCCT CCAGCACATC CAGACCCTGG ACCCCGGGCA GCACGGGGCG 
GTGGGCTATC TCGTGCAGCA TACCTTAGAG CACATTGAGC GTAAGAAGGA GGAGGTGGGC 
GTGGAGGTGA AGCTGCGCTC CGAGGAGAAG CACAGGGATG TCTGCTACTC CATCGGGCTT 
GTGATGAAGC ATAAGAGGTA CGGCTACAAC TGTGTGATCT ACGGCTGGGA CCCTACGTGC 
ATGATGGGGC ATGAGTGGAT CCGAAACATG AATGTGCACA GCCTGCCTCA TGGCCACCAT 
CAGCCCTTCT ACAACGTCCT GGTGGAGGAT GGCTCCTGCA GATATGCAGC CCAAGAAAAC 
CTGGAATACA ACGTGGAGCC TCAGGAAATC TCGCACCCAG ATGTGGGGCG CTACTTCTCG 
GAGTTCACAG GCACACACTA CATCCCAAAT GCAGAGCTGG AGATCCGGTA CCCCGAGGAC 
CTGGAGTTTG TGTACGAGAC AGTGCAGAAT ATTTACAGTG CAAAGGAGGA CACAGCCGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333517.1
CDS	32..1894
Translation	MASVAGDSAMEVVPALAEEAAAEATGPSCLVQLPGEVLEYILCSGSLTALDIGRVSSTCRRLREVCQSSGQVWKEQFRVRWPSLMKHYSPTDYVNWLEEYKVRQKAGLEARKIVASFSKRFFSEHVPCNGFSDIENLEGPEIFFEDELVCILNMEGRKALTWKYYAKKILYYLRQQKILNNLKAFLQQPDDYESYLEGAVYIDQYCNPLSDISFRDIQAQIHSIVELVCKTLRGINSRHPSLTFRAGESSMIMEIELQSQVLDAINYVLYDQLKFKGNRMDYYNALNLYMHQVLTRRTGIPISMSLLYLTVARQLGVPLEPVNFPSHFLLRWCQGAEGATLDIFDYIYIDAFGKGKQLTVKECEYLIGQHVTAALYGVVNVKKVLQRMVGNLLSLGKREGIDQSYQLLRDSLDLYLAMYPDQVQLLLLQARLYFHLGIWPEKVLDILQHIQTLDPGQHGAVGYLVQHTLEHIERKKEEVGVEVKLRSEEKHRDVCYSIGLVMKHKRYGYNCVIYGWDPTCMMGHEWIRNMNVHSLPHGHHQPFYNVLVEDGSCRYAAQENLEYNVEPQEISHPDVGRYFSEFTGTHYIPNAELEIRYPEDLEFVYETVQNIYSAKEDTAE

Target ORF information:

RefSeq Version	NM_001346588.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus F-box protein 21 (Fbxo21), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346588.1

ORF Insert Sequence:

ATGGCGTCGG TAGCGGGGGA CAGCGCTATG GAGGTGGTGC CGGCGCTGGC CGAGGAGGCC 
GCGGCTGAGG CGACGGGCCC CAGCTGCCTG GTGCAGCTGC CGGGCGAGGT GCTGGAGTAC 
ATCCTGTGCA GCGGCTCGCT CACGGCGCTG GACATCGGCC GCGTGTCCAG CACCTGCCGC 
CGCCTGCGCG AGGTGTGCCA GAGCAGCGGG CAGGTGTGGA AGGAGCAGTT CCGGGTGAGG 
TGGCCCTCCC TGATGAAGCA CTACAGCCCC ACCGACTACG TCAATTGGTT AGAGGAATAT 
AAAGTTCGGC AGAAAGCTGG GTTAGAAGCC CGGAAGATTG TAGCTTCCTT CTCCAAGCGG 
TTCTTTTCAG AGCACGTCCC CTGTAATGGC TTCAGTGACA TTGAAAACCT TGAGGGCCCA 
GAGATCTTTT TTGAGGATGA GCTGGTGTGT ATCCTAAACA TGGAAGGAAG GAAAGCTTTG 
ACCTGGAAAT ACTACGCAAA GAAAATTCTC TACTACCTGC GGCAGCAGAA AATCCTCAAT 
AACCTCAAGG CGTTCCTGCA GCAGCCCGAC GATTACGAGT CCTATCTCGA AGGTGCCGTT 
TACATCGACC AGTACTGCAA CCCACTCTCC GACATCAGCT TCAGAGACAT CCAGGCCCAG 
ATCCACAGCA TTGTGGAGCT TGTGTGCAAA ACCCTCCGTG GCATCAACAG CCGCCACCCC 
AGCCTGACCT TCAGGGCAGG TGAGTCCTCT ATGATAATGG AGATAGAACT TCAGAGCCAA 
GTGCTGGACG CTATCAACTA TGTCCTGTAT GACCAGCTGA AGTTCAAAGG GAACCGCATG 
GACTACTATA ATGCCTTGAA CCTCTACATG CACCAGGTGT TGACCCGCAG AACAGGAATC 
CCCATCAGCA TGTCTCTGCT CTACCTGACC GTCGCCCGGC AGCTGGGGGT TCCCCTGGAG 
CCTGTCAACT TCCCAAGCCA CTTCCTGCTG CGGTGGTGCC AAGGTGCAGA AGGGGCAACC 
CTGGACATCT TTGACTACAT CTACATAGAT GCTTTTGGGA AGGGCAAGCA GCTGACAGTG 
AAAGAGTGTG AGTACCTGAT TGGCCAGCAC GTGACGGCAG CACTCTACGG CGTGGTGAAT 
GTGAAGAAGG TGCTACAGCG GATGGTGGGC AACTTGCTGA GCCTGGGCAA GAGGGAAGGC 
ATTGACCAGT CCTACCAGCT CCTCAGAGAC TCCTTGGACC TGTACCTGGC GATGTACCCG 
GACCAGGTGC AGCTCCTGCT CCTCCAAGCC AGACTCTACT TCCACCTGGG CATCTGGCCA 
GAGAAGGTGC TTGACATCCT CCAGCACATC CAGACCCTGG ACCCCGGGCA GCACGGGGCG 
GTGGGCTATC TCGTGCAGCA TACCTTAGAG CACATTGAGC GTAAGAAGGA GGAGGTGGGC 
GTGGAGGTGA AGCTGCGCTC CGAGGAGAAG CACAGGGATG TCTGCTACTC CATCGGGCTT 
GTGATGAAGC ATAAGAGGTA CGGCTACAAC TGTGTGATCT ACGGCTGGGA CCCTACGTGC 
ATGATGGGGC ATGAGTGGAT CCGAAACATG AATGTGCACA GCCTGCCTCA TGGCCACCAT 
CAGCCCTTCT ACAACGTCCT GGTGGAGGAT GGCTCCTGCA GATATGCAGC CCAAGAAAAC 
CTGGAATACA ACGTGGAGCC TCAGGAAATC TCGCACCCAG ATGTGGGGCG CTACTTCTCG 
GAGTTCACAG GCACACACTA CATCCCAAAT GCAGAGCTGG AGATCCGGTA CCCCGAGGAC 
CTGGAGTTTG TGTACGAGAC AGTGCAGAAT ATTTACAGTG CAAAGGAGGA CACAGCCGAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lys29-linkage of ASK1 by Skp1-Cullin 1-Fbxo21 ubiquitin ligase complex is required for antiviral innate response.
eLife5(2016 04)
Yu Z,Chen T,Li X,Yang M,Tang S,Zhu X,Gu Y,Su X,Xia M,Li W,Zhang X,Wang Q,Cao X,Wang J

Systematic analysis and nomenclature of mammalian F-box proteins.
Genes & development18(21)2573-80(2004 Nov)
Jin J,Cardozo T,Lovering RC,Elledge SJ,Pagano M,Harper JW

Construction of a multi-functional cDNA library specific for mouse pancreatic islets and its application to microarray.
DNA research : an international journal for rapid publication of reports on genes and genomes11(5)315-23(2004 Oct)
Nishimura M,Yokoi N,Miki T,Horikawa Y,Yoshioka H,Takeda J,Ohara O,Seino S

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Prediction of the coding sequences of mouse homologues of KIAA gene: I. The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries.
DNA research : an international journal for rapid publication of reports on genes and genomes9(5)179-88(2002 Oct)
Okazaki N,Kikuno R,Ohara R,Inamoto S,Hara Y,Nagase T,Ohara O,Koga H

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Fbxo21 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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