Ppp1r14b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp1r14b
Entrez Gene ID	18938
Full Name	protein phosphatase 1, regulatory (inhibitor) subunit 14B
Synonyms	AOM172,PHI-1,PLCB3N,Png
General protein information	Preferred Names protein phosphatase 1, regulatory (inhibitor) subunit 14B Names protein phosphatase 1 regulatory subunit 14B phosphatase holoenzyme inhibitor 1 phospholipase C-beta-3 neighbouring gene protein phospholipase c neighboring
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 19 Map Location: 19\|19 A

mRNA and Protein(s)

mRNA	Protein	Name
NM_008889.2	NP_032915.2	protein phosphatase 1 regulatory subunit 14B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPP1R14B	XP_533244.2
Mus musculus (house mouse)	Ppp1r14b	NP_032915.2
Rattus norvegicus (Norway rat)	Ppp1r14b	NP_742042.1
Danio rerio (zebrafish)	ppp1r14bb	NP_001002692.1
Danio rerio (zebrafish)	ppp1r14ba	NP_001039318.1
Homo sapiens (human)	PPP1R14B	NP_619634.1
Pan troglodytes (chimpanzee)	PPP1R14B	XP_003313096.1
Xenopus tropicalis (tropical clawed frog)	ppp1r14b	NP_001016307.1

Gene Ontology
Bibliography

Related articles in PubMed

Constitutive phosphorylation of inhibitor-1 at Ser67 and Thr75 depresses calcium cycling in cardiomyocytes and leads to remodeling upon aging.
Florea S, Anjak A, Cai WF, Qian J, Vafiadaki E, Figueria S, Haghighi K, Rubinstein J, Lorenz J, Kranias EG
Basic research in cardiology107(5)279(2012 Sep)

Genomic analysis of mouse retinal development.
Blackshaw S, Harpavat S, Trimarchi J, Cai L, Huang H, Kuo WP, Weber G, Lee K, Fraioli RE, Cho SH, Yung R, Asch E, Ohno-Machado L, Wong WH, Cepko CL
PLoS biology2(9)E247(2004 Sep)

Juxtamembrane localization of the protein phosphatase-1 inhibitor protein PHI-1 in smooth muscle cells.
Tountas NA, Mandell JW, Everett AD, Brautigan DL
Histochemistry and cell biology121(4)343-50(2004 Apr)

Phosphorylation of the myosin phosphatase inhibitors, CPI-17 and PHI-1, by integrin-linked kinase.
Deng JT, Sutherland C, Brautigan DL, Eto M, Walsh MP
The Biochemical journal367(Pt 2)517-24(2002 Oct)

A novel phosphoprotein inhibitor of protein type-1 phosphatase holoenzymes.
Eto M, Karginov A, Brautigan DL
Biochemistry38(51)16952-7(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ppp1r14b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp1r14b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03010	NM_008889.2 Latest version!	Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14B (Ppp1r14b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp1r14b ( NM_008889.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032915.2 Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14B (Ppp1r14b), mRNA.

Price & Availability↑

CloneID	OMu03010
Clone ID Related Accession (Same CDS sequence)	NM_008889.2
Accession Version	NM_008889.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1569081600000
Organism	Mus musculus(house mouse)
Product	protein phosphatase 1 regulatory subunit 14B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC082545.1 and CF739461.1. On or before Dec 7, 2005 this sequence version replaced XM_913032.1, NM_008889.1. ##Evidence-Data-START## Transcript exon combination :: BC082545.1, X97490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACA GCGGCCCCGC GGGGGGCGCG GCGTTAGCTG CCCCGGCCCC CGGGCCGGGC 
AGTGGCAGCA CAGGGCCCCG AGTCTACTTC CAGAGTCCCC CTGGGGCCGC AGGCGAAGGC 
CCGGGGGGCG CTGATGACGA TGGCCCGGTG AGACGGCAAG GGAAGGTCAC CGTCAAGTAC 
GACCGCAAGG AGCTACGGAA GCGCCTCAAC CTGGAGGAGT GGATTTTAGA ACAGCTCACG 
CGACTCTACG ACTGCCAGGA GGAGGAGATC CCGGAGCTCG AGATAGATGT GGATGAACTT 
TTAGACATGG AGAGTGATGA TACCCGGGCT GCTAGGGTCA AGGAGCTGTT AGTTGACTGT 
TACAAACCCA CTGAGGCCTT CATCTCTGGC CTGCTGGACA AGATCCGGGG CATGCAGAAG 
CTGAGCACAC CCCAGAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032915.2
CDS	202..645
Translation	MADSGPAGGAALAAPAPGPGSGSTGPRVYFQSPPGAAGEGPGGADDDGPVRRQGKVTVKYDRKELRKRLNLEEWILEQLTRLYDCQEEEIPELEIDVDELLDMESDDTRAARVKELLVDCYKPTEAFISGLLDKIRGMQKLSTPQKK

Target ORF information:

RefSeq Version	NM_008889.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protein phosphatase 1, regulatory inhibitor subunit 14B (Ppp1r14b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008889.2

ORF Insert Sequence:

ATGGCGGACA GCGGCCCCGC GGGGGGCGCG GCGTTAGCTG CCCCGGCCCC CGGGCCGGGC 
AGTGGCAGCA CAGGGCCCCG AGTCTACTTC CAGAGTCCCC CTGGGGCCGC AGGCGAAGGC 
CCGGGGGGCG CTGATGACGA TGGCCCGGTG AGACGGCAAG GGAAGGTCAC CGTCAAGTAC 
GACCGCAAGG AGCTACGGAA GCGCCTCAAC CTGGAGGAGT GGATTTTAGA ACAGCTCACG 
CGACTCTACG ACTGCCAGGA GGAGGAGATC CCGGAGCTCG AGATAGATGT GGATGAACTT 
TTAGACATGG AGAGTGATGA TACCCGGGCT GCTAGGGTCA AGGAGCTGTT AGTTGACTGT 
TACAAACCCA CTGAGGCCTT CATCTCTGGC CTGCTGGACA AGATCCGGGG CATGCAGAAG 
CTGAGCACAC CCCAGAAGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Constitutive phosphorylation of inhibitor-1 at Ser67 and Thr75 depresses calcium cycling in cardiomyocytes and leads to remodeling upon aging.
Basic research in cardiology107(5)279(2012 Sep)
Florea S,Anjak A,Cai WF,Qian J,Vafiadaki E,Figueria S,Haghighi K,Rubinstein J,Lorenz J,Kranias EG

Genomic analysis of mouse retinal development.
PLoS biology2(9)E247(2004 Sep)
Blackshaw S,Harpavat S,Trimarchi J,Cai L,Huang H,Kuo WP,Weber G,Lee K,Fraioli RE,Cho SH,Yung R,Asch E,Ohno-Machado L,Wong WH,Cepko CL

Juxtamembrane localization of the protein phosphatase-1 inhibitor protein PHI-1 in smooth muscle cells.
Histochemistry and cell biology121(4)343-50(2004 Apr)
Tountas NA,Mandell JW,Everett AD,Brautigan DL

Phosphorylation of the myosin phosphatase inhibitors, CPI-17 and PHI-1, by integrin-linked kinase.
The Biochemical journal367(Pt 2)517-24(2002 Oct)
Deng JT,Sutherland C,Brautigan DL,Eto M,Walsh MP

A novel phosphoprotein inhibitor of protein type-1 phosphatase holoenzymes.
Biochemistry38(51)16952-7(1999 Dec)
Eto M,Karginov A,Brautigan DL