Mfng cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mfng
Entrez Gene ID	17305
Full Name	MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Synonyms	AW546563
General protein information	Preferred Names MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase Names beta-1,3-N-acetylglucosaminyltransferase manic fringe O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase manic fringe homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15 E1\|15 37.69 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_008595.2	NP_032621.1	beta-1,3-N-acetylglucosaminyltransferase manic fringe
XM_017316492.1	XP_017171981.1	beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X1
XM_017316492.2	XP_017171981.1	beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X1
XM_006520551.1	XP_006520614.1	beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X2
XM_006520551.2	XP_006520614.1	beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X2
XM_006520552.3	XP_006520615.1	beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mfng	NP_032621.1
Gallus gallus (chicken)	MFNG	XP_416278.2
Rattus norvegicus (Norway rat)	Mfng	NP_954541.1
Canis lupus familiaris (dog)	MFNG	XP_852869.1
Homo sapiens (human)	MFNG	NP_002396.2
Pan troglodytes (chimpanzee)	MFNG	NP_001012433.1
Bos taurus (cattle)	MFNG	NP_001094521.1
Danio rerio (zebrafish)	mfng	NP_001007789.1

Gene Ontology
Bibliography

Related articles in PubMed

Manic Fringe deficiency imposes Jagged1 addiction to intestinal tumor cells.
López-Arribillaga E, Rodilla V, Colomer C, Vert A, Shelton A, Cheng JH, Yan B, Gonzalez-Perez A, Junttila MR, Iglesias M, Torres F, Albanell J, Villanueva A, Bigas A, Siebel CW, Espinosa L
Nature communications9(1)2992(2018 07)

Radical and lunatic fringes modulate notch ligands to support mammalian intestinal homeostasis.
Kadur Lakshminarasimha Murthy P, Srinivasan T, Bochter MS, Xi R, Varanko AK, Tung KL, Semerci F, Xu K, Maletic-Savatic M, Cole SE, Shen X
eLife7(2018 04)

Repression by PRDM13 is critical for generating precision in neuronal identity.
Mona B, Uruena A, Kollipara RK, Ma Z, Borromeo MD, Chang JC, Johnson JE
eLife6(2017 08)

Deciphering the Fringe-Mediated Notch Code: Identification of Activating and Inhibiting Sites Allowing Discrimination between Ligands.
Kakuda S, Haltiwanger RS
Developmental cell40(2)193-201(2017 01)

Fine-tuning of Notch signaling sets the boundary of the organ of Corti and establishes sensory cell fates.
Basch ML, Brown RM 2nd, Jen HI, Semerci F, Depreux F, Edlund RK, Zhang H, Norton CR, Gridley T, Cole SE, Doetzlhofer A, Maletic-Savatic M, Segil N, Groves AK
eLife5(2016 12)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mfng gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mfng cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02303	NM_008595.2 Latest version!	Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu51026	XM_006520551.2 Latest version!	Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu51026	XM_006520552.3 Latest version!	Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OMu79051	XM_017316492.2 Latest version!	Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu79051	XM_017316492.1	Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu51026	XM_006520551.1	Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mfng ( NM_008595.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032621.1 Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), mRNA.

Price & Availability↑

CloneID	OMu02303
Clone ID Related Accession (Same CDS sequence)	NM_008595.2
Accession Version	NM_008595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568044800000
Organism	Mus musculus(house mouse)
Product	beta-1,3-N-acetylglucosaminyltransferase manic fringe
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL592169.14 and AL591946.14. On Nov 17, 2006 this sequence version replaced NM_008595.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U94349.1, BC010983.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGCACTGCC GACTTTTTCG GGGCATGGCG GGAGCCCTCT TTACCCTCCT GTGCGTGGGG 
CTCCTGTCTC TACGATACCA CTCAAGTTTG TCCCAGAGGA TGATACAGGG CGCGCTCAGG 
CTGAACCAAC GGAACCCAGG ACCCCTGGAG CTGCAGCTAG GCGACATCTT CATCGCAGTC 
AAGACTACCT GGGCCTTCCA TCGCTCCCGC CTGGACCTGC TACTAGACAC GTGGGTCTCC 
AGGATCAGGC AACAGACATT CATCTTCACT GACAGCCCAG ATGAACGCCT CCAGGAGAGA 
CTAGGCCCGC ACCTCGTGGT CACCAACTGT TCTGCAGAGC ACAGTCATCC TGCTCTGTCC 
TGCAAGATGG CTGCAGAGTT CGATGCCTTC TTGGTCAGTG GCCTCAGGTG GTTCTGCCAC 
GTGGATGATG ACAACTATGT GAACCCCAAG GCTCTGCTGC AGCTGTTGAA AACATTCCCG 
CAGGACCGTG ATGTCTATGT GGGCAAGCCC AGCCTGAACC GGCCCATCCA CGCCTCTGAG 
CTGCAGTCAA AAAACCGCAC GAAGCTGGTG CGGTTCTGGT TTGCCACAGG GGGTGCTGGT 
TTCTGCATCA ACCGCCAACT GGCTTTGAAG ATGGTGCCAT GGGCCAGCGG CTCCCACTTT 
GTGGACACTT CTGCTCTCAT CCGGCTCCCC GATGACTGCA CTGTGGGCTA CATCATCGAG 
TGCAAGCTGG GGGGTCGCCT GCAGCCCAGC CCCCTCTTCC ACTCACACCT GGAAACCCTG 
CAGCTGCTGG GGGCCGCCCA GCTTCCGGAG CAGGTCACCC TCAGCTACGG TGTCTTTGAG 
GGGAAACTGA ATGTCATCAA GCTACCGGGC CCCTTCTCCC ATGAAGAGGA CCCCTCCAGA 
TTCCGCTCCC TCCATTGTCT CCTCTACCCA GACACACCCT GGTGTCCGCT GCTGGCAGCG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032621.1
CDS	174..1139
Translation	MHCRLFRGMAGALFTLLCVGLLSLRYHSSLSQRMIQGALRLNQRNPGPLELQLGDIFIAVKTTWAFHRSRLDLLLDTWVSRIRQQTFIFTDSPDERLQERLGPHLVVTNCSAEHSHPALSCKMAAEFDAFLVSGLRWFCHVDDDNYVNPKALLQLLKTFPQDRDVYVGKPSLNRPIHASELQSKNRTKLVRFWFATGGAGFCINRQLALKMVPWASGSHFVDTSALIRLPDDCTVGYIIECKLGGRLQPSPLFHSHLETLQLLGAAQLPEQVTLSYGVFEGKLNVIKLPGPFSHEEDPSRFRSLHCLLYPDTPWCPLLAAP

Target ORF information:

RefSeq Version	NM_008595.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008595.2

ORF Insert Sequence:

ATGCACTGCC GACTTTTTCG GGGCATGGCG GGAGCCCTCT TTACCCTCCT GTGCGTGGGG 
CTCCTGTCTC TACGATACCA CTCAAGTTTG TCCCAGAGGA TGATACAGGG CGCGCTCAGG 
CTGAACCAAC GGAACCCAGG ACCCCTGGAG CTGCAGCTAG GCGACATCTT CATCGCAGTC 
AAGACTACCT GGGCCTTCCA TCGCTCCCGC CTGGACCTGC TACTAGACAC GTGGGTCTCC 
AGGATCAGGC AACAGACATT CATCTTCACT GACAGCCCAG ATGAACGCCT CCAGGAGAGA 
CTAGGCCCGC ACCTCGTGGT CACCAACTGT TCTGCAGAGC ACAGTCATCC TGCTCTGTCC 
TGCAAGATGG CTGCAGAGTT CGATGCCTTC TTGGTCAGTG GCCTCAGGTG GTTCTGCCAC 
GTGGATGATG ACAACTATGT GAACCCCAAG GCTCTGCTGC AGCTGTTGAA AACATTCCCG 
CAGGACCGTG ATGTCTATGT GGGCAAGCCC AGCCTGAACC GGCCCATCCA CGCCTCTGAG 
CTGCAGTCAA AAAACCGCAC GAAGCTGGTG CGGTTCTGGT TTGCCACAGG GGGTGCTGGT 
TTCTGCATCA ACCGCCAACT GGCTTTGAAG ATGGTGCCAT GGGCCAGCGG CTCCCACTTT 
GTGGACACTT CTGCTCTCAT CCGGCTCCCC GATGACTGCA CTGTGGGCTA CATCATCGAG 
TGCAAGCTGG GGGGTCGCCT GCAGCCCAGC CCCCTCTTCC ACTCACACCT GGAAACCCTG 
CAGCTGCTGG GGGCCGCCCA GCTTCCGGAG CAGGTCACCC TCAGCTACGG TGTCTTTGAG 
GGGAAACTGA ATGTCATCAA GCTACCGGGC CCCTTCTCCC ATGAAGAGGA CCCCTCCAGA 
TTCCGCTCCC TCCATTGTCT CCTCTACCCA GACACACCCT GGTGTCCGCT GCTGGCAGCG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfng ( XM_006520551.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520614.1 Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu51026
Clone ID Related Accession (Same CDS sequence)	XM_006520551.1 , XM_006520551.2 , XM_006520552.3
Accession Version	XM_006520551.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG AGTTCGATGC CTTCTTGGTC AGTGGCCTCA GGTGGTTCTG CCACGTGGAT 
GATGACAACT ATGTGAACCC CAAGGCTCTG CTGCAGCTGT TGAAAACATT CCCGCAGGAC 
CGTGATGTCT ATGTGGGCAA GCCCAGCCTG AACCGGCCCA TCCACGCCTC TGAGCTGCAG 
TCAAAAAACC GCACGAAGCT GGTGCGGTTC TGGTTTGCCA CAGGGGGTGC TGGTTTCTGC 
ATCAACCGCC AACTGGCTTT GAAGATGGTG CCATGGGCCA GCGGCTCCCA CTTTGTGGAC 
ACTTCTGCTC TCATCCGGCT CCCCGATGAC TGCACTGTGG GCTACATCAT CGAGTGCAAG 
CTGGGGGGTC GCCTGCAGCC CAGCCCCCTC TTCCACTCAC ACCTGGAAAC CCTGCAGCTG 
CTGGGGGCCG CCCAGCTTCC GGAGCAGGTC ACCCTCAGCT ACGGTGTCTT TGAGGGGAAA 
CTGAATGTCA TCAAGCTACC GGGCCCCTTC TCCCATGAAG AGGACCCCTC CAGATTCCGC 
TCCCTCCATT GTCTCCTCTA CCCAGACACA CCCTGGTGTC CGCTGCTGGC AGCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520614.1
CDS	212..811
Translation	MAAEFDAFLVSGLRWFCHVDDDNYVNPKALLQLLKTFPQDRDVYVGKPSLNRPIHASELQSKNRTKLVRFWFATGGAGFCINRQLALKMVPWASGSHFVDTSALIRLPDDCTVGYIIECKLGGRLQPSPLFHSHLETLQLLGAAQLPEQVTLSYGVFEGKLNVIKLPGPFSHEEDPSRFRSLHCLLYPDTPWCPLLAAP

Target ORF information:

RefSeq Version	XM_006520551.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520551.1

ORF Insert Sequence:

ATGGCTGCAG AGTTCGATGC CTTCTTGGTC AGTGGCCTCA GGTGGTTCTG CCACGTGGAT 
GATGACAACT ATGTGAACCC CAAGGCTCTG CTGCAGCTGT TGAAAACATT CCCGCAGGAC 
CGTGATGTCT ATGTGGGCAA GCCCAGCCTG AACCGGCCCA TCCACGCCTC TGAGCTGCAG 
TCAAAAAACC GCACGAAGCT GGTGCGGTTC TGGTTTGCCA CAGGGGGTGC TGGTTTCTGC 
ATCAACCGCC AACTGGCTTT GAAGATGGTG CCATGGGCCA GCGGCTCCCA CTTTGTGGAC 
ACTTCTGCTC TCATCCGGCT CCCCGATGAC TGCACTGTGG GCTACATCAT CGAGTGCAAG 
CTGGGGGGTC GCCTGCAGCC CAGCCCCCTC TTCCACTCAC ACCTGGAAAC CCTGCAGCTG 
CTGGGGGCCG CCCAGCTTCC GGAGCAGGTC ACCCTCAGCT ACGGTGTCTT TGAGGGGAAA 
CTGAATGTCA TCAAGCTACC GGGCCCCTTC TCCCATGAAG AGGACCCCTC CAGATTCCGC 
TCCCTCCATT GTCTCCTCTA CCCAGACACA CCCTGGTGTC CGCTGCTGGC AGCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfng ( XM_006520551.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520614.1 Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu51026
Clone ID Related Accession (Same CDS sequence)	XM_006520551.1 , XM_006520551.2 , XM_006520552.3
Accession Version	XM_006520551.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006520551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG AGTTCGATGC CTTCTTGGTC AGTGGCCTCA GGTGGTTCTG CCACGTGGAT 
GATGACAACT ATGTGAACCC CAAGGCTCTG CTGCAGCTGT TGAAAACATT CCCGCAGGAC 
CGTGATGTCT ATGTGGGCAA GCCCAGCCTG AACCGGCCCA TCCACGCCTC TGAGCTGCAG 
TCAAAAAACC GCACGAAGCT GGTGCGGTTC TGGTTTGCCA CAGGGGGTGC TGGTTTCTGC 
ATCAACCGCC AACTGGCTTT GAAGATGGTG CCATGGGCCA GCGGCTCCCA CTTTGTGGAC 
ACTTCTGCTC TCATCCGGCT CCCCGATGAC TGCACTGTGG GCTACATCAT CGAGTGCAAG 
CTGGGGGGTC GCCTGCAGCC CAGCCCCCTC TTCCACTCAC ACCTGGAAAC CCTGCAGCTG 
CTGGGGGCCG CCCAGCTTCC GGAGCAGGTC ACCCTCAGCT ACGGTGTCTT TGAGGGGAAA 
CTGAATGTCA TCAAGCTACC GGGCCCCTTC TCCCATGAAG AGGACCCCTC CAGATTCCGC 
TCCCTCCATT GTCTCCTCTA CCCAGACACA CCCTGGTGTC CGCTGCTGGC AGCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520614.1
CDS	1146..1745
Translation	MAAEFDAFLVSGLRWFCHVDDDNYVNPKALLQLLKTFPQDRDVYVGKPSLNRPIHASELQSKNRTKLVRFWFATGGAGFCINRQLALKMVPWASGSHFVDTSALIRLPDDCTVGYIIECKLGGRLQPSPLFHSHLETLQLLGAAQLPEQVTLSYGVFEGKLNVIKLPGPFSHEEDPSRFRSLHCLLYPDTPWCPLLAAP

Target ORF information:

RefSeq Version	XM_006520551.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520551.2

ORF Insert Sequence:

ATGGCTGCAG AGTTCGATGC CTTCTTGGTC AGTGGCCTCA GGTGGTTCTG CCACGTGGAT 
GATGACAACT ATGTGAACCC CAAGGCTCTG CTGCAGCTGT TGAAAACATT CCCGCAGGAC 
CGTGATGTCT ATGTGGGCAA GCCCAGCCTG AACCGGCCCA TCCACGCCTC TGAGCTGCAG 
TCAAAAAACC GCACGAAGCT GGTGCGGTTC TGGTTTGCCA CAGGGGGTGC TGGTTTCTGC 
ATCAACCGCC AACTGGCTTT GAAGATGGTG CCATGGGCCA GCGGCTCCCA CTTTGTGGAC 
ACTTCTGCTC TCATCCGGCT CCCCGATGAC TGCACTGTGG GCTACATCAT CGAGTGCAAG 
CTGGGGGGTC GCCTGCAGCC CAGCCCCCTC TTCCACTCAC ACCTGGAAAC CCTGCAGCTG 
CTGGGGGCCG CCCAGCTTCC GGAGCAGGTC ACCCTCAGCT ACGGTGTCTT TGAGGGGAAA 
CTGAATGTCA TCAAGCTACC GGGCCCCTTC TCCCATGAAG AGGACCCCTC CAGATTCCGC 
TCCCTCCATT GTCTCCTCTA CCCAGACACA CCCTGGTGTC CGCTGCTGGC AGCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfng ( XM_006520552.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006520615.1 Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu51026
Clone ID Related Accession (Same CDS sequence)	XM_006520551.1 , XM_006520551.2 , XM_006520552.3
Accession Version	XM_006520552.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006520552.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG AGTTCGATGC CTTCTTGGTC AGTGGCCTCA GGTGGTTCTG CCACGTGGAT 
GATGACAACT ATGTGAACCC CAAGGCTCTG CTGCAGCTGT TGAAAACATT CCCGCAGGAC 
CGTGATGTCT ATGTGGGCAA GCCCAGCCTG AACCGGCCCA TCCACGCCTC TGAGCTGCAG 
TCAAAAAACC GCACGAAGCT GGTGCGGTTC TGGTTTGCCA CAGGGGGTGC TGGTTTCTGC 
ATCAACCGCC AACTGGCTTT GAAGATGGTG CCATGGGCCA GCGGCTCCCA CTTTGTGGAC 
ACTTCTGCTC TCATCCGGCT CCCCGATGAC TGCACTGTGG GCTACATCAT CGAGTGCAAG 
CTGGGGGGTC GCCTGCAGCC CAGCCCCCTC TTCCACTCAC ACCTGGAAAC CCTGCAGCTG 
CTGGGGGCCG CCCAGCTTCC GGAGCAGGTC ACCCTCAGCT ACGGTGTCTT TGAGGGGAAA 
CTGAATGTCA TCAAGCTACC GGGCCCCTTC TCCCATGAAG AGGACCCCTC CAGATTCCGC 
TCCCTCCATT GTCTCCTCTA CCCAGACACA CCCTGGTGTC CGCTGCTGGC AGCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006520615.1
CDS	172..771
Translation	MAAEFDAFLVSGLRWFCHVDDDNYVNPKALLQLLKTFPQDRDVYVGKPSLNRPIHASELQSKNRTKLVRFWFATGGAGFCINRQLALKMVPWASGSHFVDTSALIRLPDDCTVGYIIECKLGGRLQPSPLFHSHLETLQLLGAAQLPEQVTLSYGVFEGKLNVIKLPGPFSHEEDPSRFRSLHCLLYPDTPWCPLLAAP

Target ORF information:

RefSeq Version	XM_006520552.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006520552.3

ORF Insert Sequence:

ATGGCTGCAG AGTTCGATGC CTTCTTGGTC AGTGGCCTCA GGTGGTTCTG CCACGTGGAT 
GATGACAACT ATGTGAACCC CAAGGCTCTG CTGCAGCTGT TGAAAACATT CCCGCAGGAC 
CGTGATGTCT ATGTGGGCAA GCCCAGCCTG AACCGGCCCA TCCACGCCTC TGAGCTGCAG 
TCAAAAAACC GCACGAAGCT GGTGCGGTTC TGGTTTGCCA CAGGGGGTGC TGGTTTCTGC 
ATCAACCGCC AACTGGCTTT GAAGATGGTG CCATGGGCCA GCGGCTCCCA CTTTGTGGAC 
ACTTCTGCTC TCATCCGGCT CCCCGATGAC TGCACTGTGG GCTACATCAT CGAGTGCAAG 
CTGGGGGGTC GCCTGCAGCC CAGCCCCCTC TTCCACTCAC ACCTGGAAAC CCTGCAGCTG 
CTGGGGGCCG CCCAGCTTCC GGAGCAGGTC ACCCTCAGCT ACGGTGTCTT TGAGGGGAAA 
CTGAATGTCA TCAAGCTACC GGGCCCCTTC TCCCATGAAG AGGACCCCTC CAGATTCCGC 
TCCCTCCATT GTCTCCTCTA CCCAGACACA CCCTGGTGTC CGCTGCTGGC AGCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfng ( XM_017316492.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171981.1 Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu79051
Clone ID Related Accession (Same CDS sequence)	XM_017316492.1 , XM_017316492.2
Accession Version	XM_017316492.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1466438400000
Organism	Mus musculus(house mouse)
Product	beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCTG GCGCTTTCCT GTCGTCTTGC TCACGGCGAT GGTGCCCTTG CCCTGAAAGG 
AAGGGCTGGC CAGAGTCACC CAGGAGGCCT ATGGGGCATA GCACAGAGAC ATTCATCTTC 
ACTGACAGCC CAGATGAACG CCTCCAGGAG AGACTAGGCC CGCACCTCGT GGTCACCAAC 
TGTTCTGCAG AGCACAGTCA TCCTGCTCTG TCCTGCAAGA TGGCTGCAGA GTTCGATGCC 
TTCTTGGTCA GTGGCCTCAG GTGGTTCTGC CACGTGGATG ATGACAACTA TGTGAACCCC 
AAGGCTCTGC TGCAGCTGTT GAAAACATTC CCGCAGGACC GTGATGTCTA TGTGGGCAAG 
CCCAGCCTGA ACCGGCCCAT CCACGCCTCT GAGCTGCAGT CAAAAAACCG CACGAAGCTG 
GTGCGGTTCT GGTTTGCCAC AGGGGGTGCT GGTTTCTGCA TCAACCGCCA ACTGGCTTTG 
AAGATGGTGC CATGGGCCAG CGGCTCCCAC TTTGTGGACA CTTCTGCTCT CATCCGGCTC 
CCCGATGACT GCACTGTGGG CTACATCATC GAGTGCAAGC TGGGGGGTCG CCTGCAGCCC 
AGCCCCCTCT TCCACTCACA CCTGGAAACC CTGCAGCTGC TGGGGGCCGC CCAGCTTCCG 
GAGCAGGTCA CCCTCAGCTA CGGTGTCTTT GAGGGGAAAC TGAATGTCAT CAAGCTACCG 
GGCCCCTTCT CCCATGAAGA GGACCCCTCC AGATTCCGCT CCCTCCATTG TCTCCTCTAC 
CCAGACACAC CCTGGTGTCC GCTGCTGGCA GCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171981.1
CDS	65..883
Translation	MVPGAFLSSCSRRWCPCPERKGWPESPRRPMGHSTETFIFTDSPDERLQERLGPHLVVTNCSAEHSHPALSCKMAAEFDAFLVSGLRWFCHVDDDNYVNPKALLQLLKTFPQDRDVYVGKPSLNRPIHASELQSKNRTKLVRFWFATGGAGFCINRQLALKMVPWASGSHFVDTSALIRLPDDCTVGYIIECKLGGRLQPSPLFHSHLETLQLLGAAQLPEQVTLSYGVFEGKLNVIKLPGPFSHEEDPSRFRSLHCLLYPDTPWCPLLAAP

Target ORF information:

RefSeq Version	XM_017316492.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316492.1

ORF Insert Sequence:

ATGGTCCCTG GCGCTTTCCT GTCGTCTTGC TCACGGCGAT GGTGCCCTTG CCCTGAAAGG 
AAGGGCTGGC CAGAGTCACC CAGGAGGCCT ATGGGGCATA GCACAGAGAC ATTCATCTTC 
ACTGACAGCC CAGATGAACG CCTCCAGGAG AGACTAGGCC CGCACCTCGT GGTCACCAAC 
TGTTCTGCAG AGCACAGTCA TCCTGCTCTG TCCTGCAAGA TGGCTGCAGA GTTCGATGCC 
TTCTTGGTCA GTGGCCTCAG GTGGTTCTGC CACGTGGATG ATGACAACTA TGTGAACCCC 
AAGGCTCTGC TGCAGCTGTT GAAAACATTC CCGCAGGACC GTGATGTCTA TGTGGGCAAG 
CCCAGCCTGA ACCGGCCCAT CCACGCCTCT GAGCTGCAGT CAAAAAACCG CACGAAGCTG 
GTGCGGTTCT GGTTTGCCAC AGGGGGTGCT GGTTTCTGCA TCAACCGCCA ACTGGCTTTG 
AAGATGGTGC CATGGGCCAG CGGCTCCCAC TTTGTGGACA CTTCTGCTCT CATCCGGCTC 
CCCGATGACT GCACTGTGGG CTACATCATC GAGTGCAAGC TGGGGGGTCG CCTGCAGCCC 
AGCCCCCTCT TCCACTCACA CCTGGAAACC CTGCAGCTGC TGGGGGCCGC CCAGCTTCCG 
GAGCAGGTCA CCCTCAGCTA CGGTGTCTTT GAGGGGAAAC TGAATGTCAT CAAGCTACCG 
GGCCCCTTCT CCCATGAAGA GGACCCCTCC AGATTCCGCT CCCTCCATTG TCTCCTCTAC 
CCAGACACAC CCTGGTGTCC GCTGCTGGCA GCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mfng ( XM_017316492.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171981.1 Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X1, mRNA.

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CloneID	OMu79051
Clone ID Related Accession (Same CDS sequence)	XM_017316492.1 , XM_017316492.2
Accession Version	XM_017316492.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1565107200000
Organism	Mus musculus(house mouse)
Product	beta-1,3-N-acetylglucosaminyltransferase manic fringe isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCCTG GCGCTTTCCT GTCGTCTTGC TCACGGCGAT GGTGCCCTTG CCCTGAAAGG 
AAGGGCTGGC CAGAGTCACC CAGGAGGCCT ATGGGGCATA GCACAGAGAC ATTCATCTTC 
ACTGACAGCC CAGATGAACG CCTCCAGGAG AGACTAGGCC CGCACCTCGT GGTCACCAAC 
TGTTCTGCAG AGCACAGTCA TCCTGCTCTG TCCTGCAAGA TGGCTGCAGA GTTCGATGCC 
TTCTTGGTCA GTGGCCTCAG GTGGTTCTGC CACGTGGATG ATGACAACTA TGTGAACCCC 
AAGGCTCTGC TGCAGCTGTT GAAAACATTC CCGCAGGACC GTGATGTCTA TGTGGGCAAG 
CCCAGCCTGA ACCGGCCCAT CCACGCCTCT GAGCTGCAGT CAAAAAACCG CACGAAGCTG 
GTGCGGTTCT GGTTTGCCAC AGGGGGTGCT GGTTTCTGCA TCAACCGCCA ACTGGCTTTG 
AAGATGGTGC CATGGGCCAG CGGCTCCCAC TTTGTGGACA CTTCTGCTCT CATCCGGCTC 
CCCGATGACT GCACTGTGGG CTACATCATC GAGTGCAAGC TGGGGGGTCG CCTGCAGCCC 
AGCCCCCTCT TCCACTCACA CCTGGAAACC CTGCAGCTGC TGGGGGCCGC CCAGCTTCCG 
GAGCAGGTCA CCCTCAGCTA CGGTGTCTTT GAGGGGAAAC TGAATGTCAT CAAGCTACCG 
GGCCCCTTCT CCCATGAAGA GGACCCCTCC AGATTCCGCT CCCTCCATTG TCTCCTCTAC 
CCAGACACAC CCTGGTGTCC GCTGCTGGCA GCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171981.1
CDS	63..881
Translation	MVPGAFLSSCSRRWCPCPERKGWPESPRRPMGHSTETFIFTDSPDERLQERLGPHLVVTNCSAEHSHPALSCKMAAEFDAFLVSGLRWFCHVDDDNYVNPKALLQLLKTFPQDRDVYVGKPSLNRPIHASELQSKNRTKLVRFWFATGGAGFCINRQLALKMVPWASGSHFVDTSALIRLPDDCTVGYIIECKLGGRLQPSPLFHSHLETLQLLGAAQLPEQVTLSYGVFEGKLNVIKLPGPFSHEEDPSRFRSLHCLLYPDTPWCPLLAAP

Target ORF information:

RefSeq Version	XM_017316492.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus MFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase (Mfng), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316492.2

ORF Insert Sequence:

ATGGTCCCTG GCGCTTTCCT GTCGTCTTGC TCACGGCGAT GGTGCCCTTG CCCTGAAAGG 
AAGGGCTGGC CAGAGTCACC CAGGAGGCCT ATGGGGCATA GCACAGAGAC ATTCATCTTC 
ACTGACAGCC CAGATGAACG CCTCCAGGAG AGACTAGGCC CGCACCTCGT GGTCACCAAC 
TGTTCTGCAG AGCACAGTCA TCCTGCTCTG TCCTGCAAGA TGGCTGCAGA GTTCGATGCC 
TTCTTGGTCA GTGGCCTCAG GTGGTTCTGC CACGTGGATG ATGACAACTA TGTGAACCCC 
AAGGCTCTGC TGCAGCTGTT GAAAACATTC CCGCAGGACC GTGATGTCTA TGTGGGCAAG 
CCCAGCCTGA ACCGGCCCAT CCACGCCTCT GAGCTGCAGT CAAAAAACCG CACGAAGCTG 
GTGCGGTTCT GGTTTGCCAC AGGGGGTGCT GGTTTCTGCA TCAACCGCCA ACTGGCTTTG 
AAGATGGTGC CATGGGCCAG CGGCTCCCAC TTTGTGGACA CTTCTGCTCT CATCCGGCTC 
CCCGATGACT GCACTGTGGG CTACATCATC GAGTGCAAGC TGGGGGGTCG CCTGCAGCCC 
AGCCCCCTCT TCCACTCACA CCTGGAAACC CTGCAGCTGC TGGGGGCCGC CCAGCTTCCG 
GAGCAGGTCA CCCTCAGCTA CGGTGTCTTT GAGGGGAAAC TGAATGTCAT CAAGCTACCG 
GGCCCCTTCT CCCATGAAGA GGACCCCTCC AGATTCCGCT CCCTCCATTG TCTCCTCTAC 
CCAGACACAC CCTGGTGTCC GCTGCTGGCA GCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Manic Fringe deficiency imposes Jagged1 addiction to intestinal tumor cells.
Nature communications9(1)2992(2018 07)
López-Arribillaga E,Rodilla V,Colomer C,Vert A,Shelton A,Cheng JH,Yan B,Gonzalez-Perez A,Junttila MR,Iglesias M,Torres F,Albanell J,Villanueva A,Bigas A,Siebel CW,Espinosa L

Radical and lunatic fringes modulate notch ligands to support mammalian intestinal homeostasis.
eLife7(2018 04)
Kadur Lakshminarasimha Murthy P,Srinivasan T,Bochter MS,Xi R,Varanko AK,Tung KL,Semerci F,Xu K,Maletic-Savatic M,Cole SE,Shen X

Repression by PRDM13 is critical for generating precision in neuronal identity.
eLife6(2017 08)
Mona B,Uruena A,Kollipara RK,Ma Z,Borromeo MD,Chang JC,Johnson JE

Deciphering the Fringe-Mediated Notch Code: Identification of Activating and Inhibiting Sites Allowing Discrimination between Ligands.
Developmental cell40(2)193-201(2017 01)
Kakuda S,Haltiwanger RS

Fine-tuning of Notch signaling sets the boundary of the organ of Corti and establishes sensory cell fates.
eLife5(2016 12)
Basch ML,Brown RM 2nd,Jen HI,Semerci F,Depreux F,Edlund RK,Zhang H,Norton CR,Gridley T,Cole SE,Doetzlhofer A,Maletic-Savatic M,Segil N,Groves AK

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Mfng cDNA ORF clone, Mus musculus(house mouse)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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