SPTA1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPTA1
Entrez Gene ID	100030300
Full Name	spectrin alpha, erythrocytic 1
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007481672.2	XP_007481734.2	LOW QUALITY PROTEIN: spectrin alpha chain, erythrocytic 1

Pathways from BioSystems
Gene Ontology

The following SPTA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMc40239	XM_007481672.2 Latest version!	Monodelphis domestica spectrin alpha, erythrocytic 1 (SPTA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPTA1 ( XM_007481672.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007481734.2 Monodelphis domestica spectrin alpha, erythrocytic 1 (SPTA1), mRNA.

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CloneID	OMc40239
Clone ID Related Accession (Same CDS sequence)	XM_007481672.2
Accession Version	XM_007481672.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	LOW QUALITY PROTEIN: spectrin alpha chain, erythrocytic 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008802.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007481672.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAGAGCA CTGGAATTAA AGTGCTGGAA ACAGCAGAAG AAATCCAGGG AAGACGACGA 
GAAGTGTTGA GTCGGTACCA GCGTTTCAAA CAGCTTGTTA CTGAACGTGG TCAAAAGCTT 
GAAGATTCCT ATCACTATCA GGTTTTCAGG CGAGATGCAG ATGACCTGGA GAAATGGATC 
TTGGAGAAAC TCAAGATTGC TTCTGATGAA AATTACAAAG ACCCAACTAA CATACAGGGG 
AAATATCAGA AACATCAATC CTTTGAAGCT GAAGTACAAG CAAAATCTAG AGTGATTCCA 
GAGCTGGAAG AAATCAGACG AGTTCGATTT GCTGAAGGAC ATTTTGCTCA TGAAGACACC 
AAGGAGCATC TGGAAGAACT GAAGAAACTG TGGGATTTGC TTTTAGAGCT GACTAAGGAG 
AAGGGCACTC GGTTGTTGCA GGTCCTGAAG CTGCAGCAGT ACTTGCAGGA GTGCGCTGAC 
ATCCTTGAGT GGATCAGTGA CAAAGAAACC ATTGTGACAT CAGTAGAACT GGGTGAAGAC 
TGGGAGAGAG CTGAAGTTCT GCACAAGAAA TTTGAGGAGT TCCAGGTGGA TCTGGCAGCT 
CGTGAGGGGC GAGTGGCTGC AGTGAATGAG TTTGCCAACC AGTGTGCCCA GGAAGATCAT 
TCGCAACTGG AACTAATCAA GACAAAGCAG GATGAAGTAA ATACCAATTG GCAGCGTCTC 
CAGGGCTTAG CTCTCCAGAG ACGAGAAACT TTGTCTGATG CTGCTGACTT GCAACGCTTC 
AACAGGGATG TAGCTGAAGC TATTCAGTGG ATCAAGGAGA AGGAGCCTCT TCTTACCTCG 
GAAGACTTTG GCAAAGATCT GGTTAGCTCT GAAGCCCTGT TCCACAGTCA CAAAGGGCTG 
GAAAGGAGCC TAGCTGTCAT GGAAGACAAG GTAAGAGAGC TGTGTGCTAA GGCAGATAAG 
CTACAGGTCT CCCATCCCTC AGATGCACGT GAGATCCAGG AGAAGAAAGA AGACCTCTCC 
TCTAACTGGG CACTAATCCG GGCTCTAGCC ACCAAAAGAT ATAAGAAGCT TCAGGCTTCA 
TACTGGTACC AACGCTTCCT CTCCGACTTT GATGAACTCA CAGGCTGGAT GGGAGAGAAA 
ACAGCCCTTA TCAATGCTGA TGAGCTGCCA ACAGATGTGG CAGGTGGGGA AGCCCTGTTA 
GACAGGCACC AACAGCATAA GCATGAAATT GATTCCTATG AAGACCGATT TCAGTCAGCT 
GCAGAAACTG GCCAGTATCT GTTGGATGCT GACCATGAGG CATCTGAAGA AGTTTGTGAA 
AAGATGACTG CTCTTGACAA CGATCACGCT GCACTGCTGG AATTATGGGA GAGACGTCGT 
CATCAATACG AGCAGTGCCT GGACCTACAC CTCTTCTATC GAGATAGTGA GCAAGTGGAC 
AGCTGGATGA GCAGACAGGA GGCATTCTTA GAGAATGAGG ACTTGGGGAA CTCTCTGGGC 
ACTGTTGAAG CCCTTCTTCA GAAGCATGAG GACTTTGAGG AGGCCTTTGG TGCCCAGGAA 
GAGAAAATTA CAACACTGGA CAAGACAGCA ACCAAACTGA TCGACAATGA CCATTATGAT 
TCAGAGAACA TCGCTGGGAT CAGGGATGGG CTTTTGGCAA GGCGAGATGC CCTCCGTGAC 
CGTGCTGCTT CCAGGCGAGG GAAGCTGAAA GATGCCCTGC TTCTGCAGCA GCTCTACCAA 
GACTCAGATG ACCTAAAGAA CTGGATCAAT AAGAAGAAAA AATTAGCAGA TGATGAGGAT 
TACAAGGACT CACAGAACCT AAAGAGCCGA GTTCAAAAGC AACAAGTATT TCAAGAGGAG 
CTGATGGCTA ATGAGGTTCG CCTCAACAAC CTGAAAAAAA CTGGCCAGGA GATGATTGAT 
GGCAACCACT ATGCCTCTGT TAATGTGACT GCCCGAATTA ATGAGGTCAC TGCCCTCTGG 
AAGGAGCTCC TAGAAACAAC AGAGCAGAAA GGATCCCAGC TGCATGAAGC CAATCAGAAG 
CTACTATTTG AACAGAATGT GGAGGATTTA GCCCGCTGGC TGGAGGAAGT GGAGGGTCAG 
GTTGCCTCTG AAGATTATGG GAAAGGCCTG GCTGATGTCC AGAATCTCCT TCGGAAACAT 
GGACTCTTAG AGACCAGTGT GAATGCTCAT CAGGACCAAG TAGACACAAT TACAGACCTA 
GCTGCCTATT TTGAGGAGGT GGGACATCCT GATGCTGAAG AAATGAAATT AAGGCAGGAA 
TCTCTGGTGT CAAGTTATGA GTCCCTGAAG GAACCAATGG ACAAAAGGAA GAGGAAGCTC 
ATTGACCTTC TGCTACTGCA ACAGATCTGC CGGGACACAG AAGATGAGGA GGCTTGGATT 
CAAGAGACAG AACCTTCTGC TGCCTCCACT TACCTTGGAA AGGACCTGAT TGCTGCTCAG 
AACCTGCTGA ATAGGCACCA GGTCATTTTG GCTGATATTG ACAGCCATGA ACCCCGAATT 
CATGCCATCA CAGAGAGAGG CAACAAATTG GTAGATGAAG GGCACTTTGC TGCAGAGGAT 
GTAGCCTCTA GAGTAAAGAG CATGAATGAG AACATGGAGT CCCTTCAGGC TCGGGCTGCC 
CAGCGACAGT GTGACCTGGA GGACAATGTC CATTTCCAGC AGTACCTTGC AGATCTACAT 
GAAGCAGAAA CCTGGATTCA GGAGAAGGAG CCCATTGTGG ACAACACAAA CTATGGGGAT 
GATGAGGAAG CTGCTGGGGC TCTTCTGAAG AAGCACGAAG CTTTCCTAGT GGACCTTAAT 
GCCTTTGGAA CCAGCATGAA GGCTCTGAGG AAGCAGGCAG ATGCATGCCG GCAACAAGCT 
GCACCTATGG AAGGGGCTGC GCAGGAAGAA CGGGTCATAG CTTTATATGA CTTCGAGGCC 
AGGAGCCCCA GAGAAGTCAC CATGAAGAAA GATGATGTCT TAACCCTGCT CAGCTCTATC 
AACAAGGACT GGTGGAAGGT AGAAGCTGAT GATCATCAGG GCTTTGTCCC AGCTGTGTAT 
GTCAGGAGAT TGGCCAGGGA TGAGCTCCCC AGGCCTGAGA GGCGGCGTCG AGAAGAGCCA 
AGTGACATTG CCCAACGCCA GGAGCAAATT GATAATCAGT ACCACTCTCT TCTGGAGAGG 
GCAGAAGACC GTCGGCGTCG CCTTTTACAG CGATACAAGG AGTTCCTGCT AGCTTATGAG 
GCTGGAGACA TGCTGGATTG GATCAGAGAG AAGAAGGAAG AAAACACTGG AGTAGAACTA 
GAAGATGTCC ATGAGCTGCA AAAAAAGTTT GATGACTTCC AGACGGATCT GAAGACCAAT 
GAACCTCGAC TTCAGGACAT TAACAAAGTG GCTGATGATC TTCGTTTAGA AGACCTTTTA 
ACTCCAGAAG GTGCTGAAAT CCAGCAGGAG TTAAATAGCC GCTGGGATTC CCTCCAGAGA 
CTTGCAGAGG AGCAGCGCCT GCTGCTGGGC AGTGCTCATG CTGTCCAAAT GTTCCACAGA 
GATGCAGAGG ACACCAAGGA ACGCATTGAG AAGAAGCATC AGGCCTTGAG TGCTGCAGAC 
CCTGGCTCAG ATCTGTTCAG TGTTCAGGCG CTTCAGCGTC GCCATGATGC TTTTGAGAGG 
GATTTGGAAC CTCTTGGAGA AAAGGTGACT ACTTTGGGAG AGACAGCAGA GAGGCTCATT 
GAGACTCACC CAGATGCCAC TGAGGACCTG CGCAGGCAAC AGGTTGAGCT TGACGGGGCC 
TGGGATAATT TGCTTCGGCG CACAGAAGAA AGAAAGGATG ACCTGAGCGA GGCGCAGAAG 
CTCTATTTGT TCCTCAATCA GGCCAGGGAA ATATTGTCGT GGATCAATAG CATTCGTGGC 
CCTGTGTCAT CAGTGGAATT GGCTGAGGAC CTCACAGGAA CTGAAATCTT ACTTGAAAGA 
CATCAGGAAC ACCATGATGA GATGAAGGCT GAAGCTCCTA CATTCCAGGC CCTAGAAGAC 
TTTGGTATCG ACCTTCTAAG CAGTGGCCAC CGGGCTGGCC CTGAGATAGA AGGGAAGTTG 
CAATTGGTGA GACTTGAAAG AGATGATTTG GAGAAATCTT GGGAGCGGCG AAAGAAGATG 
CTTGATCAAT GTCTGGATTT CCAGCTGTTC CAAGGAGATT GTGAGCAAGC AGAGAGCTGG 
ATGGTGGCCC GAGAGAGTTC TCTGAGGACA GATGACAAAA GCTCACTGAG TAGTCTGGAG 
GCCTTGATGA AGAAGCGTGA TGATTTAGAT AAGGCAATCA ATGCCCAGGA AGAAAAGATA 
AATTCACTGA AAAGTTTTGC AGAGCGGCTC ATAGATGATG ACCACTATGC CAAGAAAGAA 
ATTGCTGACC GGCTTCAGAG AGTGTTGGAC AGGTGGAATG CTCTCAAAGG ACAGATGATT 
GCAGAAAGAG AACAGCTTAG TGATTATGCA GACCTGAAAC AATTCTACCG TGACATTGAA 
GATTTGTCAG ATTGGATCAA TGAGATGATG CCAACAGCCT GTGATGAGTC CTACAAAGAT 
CCCACTAATA TCCAGAGAAA ATACTTGAAG CATCAGACAT TTGAAAATGA AGTCTTCGGC 
CGGAATGATG AAGTCGAAAA TGTCATCAAA CTGGGGAACT CCCTTATTGA TCGCAGAGCA 
TGTGATGGCC AGGAGGAAGA TGTGAAGGAA CGAGTGTCTA GACTTAGTGA ACACTGGAAC 
CAGCTCCTGG AGAAAACCAA AGACAAAGGG CAGAAATTGA ACGAGGCAAG CCGGCAACAG 
AGGTTCAACA CAGGCATCCG TGACTTTGAA TTCTGGCTCT CTGAGGCAGA GACACTACTG 
GCGATGAAAG ATCAAGCCAG GGACTTGGCT TCAGCAGCCA ACTTACTTAA GAAGCATCAA 
CTGTTGGAAA CAGAAATGTC AGCTCGTGAG GACTTTCTCA AAGATCTGAA TGAACTGGCT 
ACTCACTTAA TCGACAGTGG GGCTTTCAAT GCTGATGAGA TCAAGGAGAA ACGGGATAAT 
ATCAATAACC GATTCCGGAA TGTCCAGGAC CTGGCTGCTG CGCATCACAA AAAGCTCAAG 
GAGGCCCATG CTCTTTTCCA GTTTTTCCAG GATCTGGATG ATGAGGAATC CTGGATTAAG 
GCAAAGCTTC TACGAGTGAG CTCCAAGGAC TATGGACGGG ATCTTCCTGG TGTTCAGAAC 
CTACAAAGGA AACATAAACG CCTGGAAGGG GAGCTGGATG CCCATGAACC AGCTATCCAG 
GGAGTGTTGG AGACTGCAGA AAAACTGAAA GACAGTGATG CTGTGGGGCG AGAGGAGATC 
CAAGAGAGGC TGGCTCAGCT GGTGCAGCAT TGGAATGAGC TGAAGGAGTT GACCAAGATT 
CGTGGGCTCC GACTAGATGA GTCCCAACAG TATCTTCAGT TCATTGAGAA TGTTGAGGAA 
GAAGAGGCCT GGATCAATGA GAAGACAGCT CTTGTTGCCC GAGGGGATAC AGGAGACATA 
TTACCTGCCA CACAGAGCTT ACTTAAGAAG CATGAAGCTT TGGAGAATGA TTTTGCAAAC 
CATGAGATTA AAGTTCAGGA AATATGCATT GAAGGAGAAG ACCTTCTGGG CAAGGAGGAC 
ACACAGCACA AAGACAAAAT CACAGCCAAG ATCAATGGTC TGAAGTCAAA GATCCCTGCT 
TTAGCCAAAG CAATGGCAGC CTGGAAGGCC CAGTTGGACG AGGATGCTGC TTTCCAATTG 
TTTAACTGGA AGGCTGATAC AGTAGAGTCC TGGATAGCTG AAAAAGAATC TTCCCTGAAG 
ACCAATGGTA ATGGAGGAGA CCTTGCTGCT TACCGTATGC TACTCACTAA GCAGGATACA 
CTGGATGATG GCCTGAAAAG CTTTGAGCAC AAACGGCTGT CAGAAATAAC AGACTTAAAA 
GACCAACTGG TGGCAGGGGA ACACAGCAGG GCAAGTACCA TCGAGGACCG GCATGCCGCC 
CTTCTCGAAC GCTGGGACCA GCTACTGGCT GCTGCTGCAG CCCGAAGGGC AAAGCTGCTG 
GAGGAGCAGC TCCCCCTTCA GAAGGCTGAA GAGTTATTCT TGGAGTTTGC CCAGAAGGCT 
TCAGCCTTCA ACAACTGGTG TGAGAATGCA GAAGAGGACC TCTCTGAACC TGTACACTGT 
GTCTCCTTAG AAGAAATCCA ACAACTTCAG AAGGACCATG AGGCCTTTCT GGTCTCCCTC 
CAGGGAGCTC GAGCTGACTT TGACCACTTG GTAGAGCTGG ATCAGCAAAT TAAGGCTTTG 
AACATGCCCT CCAGTCCCTA TACCTGGTTA ACCGTGGAAG TTCTAGAAGG CATCTGGTTG 
AATCTGCCCA ATATCATCAA GGAAAGAGAG CAGGAATTGC AAAAAGAGGA AGAGAGACAA 
ATGAAGAATC GTGAGCTCTG CCAAGAGTTT GAACAAGAGG CCAGTGCATT CTCCCAGTGG 
ATCCAGGAAA CCAGAGCTTA TTTCCTAGAT GGATCATTGC TCAAGGAAAC TGGAACCCTA 
GAATCCCAGC TGGAAGCTAA TAAACGGAAG CAGAAAGAAA TTCAAGTGAT GAAGCGTCAG 
TTGAACAAGA TTGAGGACCT GGGAGATATC ATGGAGGATG CTCTGATCTT GGACATCAAA 
TACAGCACCA TTGGATTAGC CCAGCAGTGG GATCAGCTCC ACCAGCTAGG AATAAGAATG 
CAACACAATC TAGAGCAACA GATCCAGGCC AAGGACATGA TTGGTGTGAG TGAAGAGACG 
CTAAAGGAAT TTAGCACAAC CTATCAACAT TTTGATGAGA CCTTGTCTGG AAGGCTGAGT 
CACAAGGAAT TCCGGTCCTG TCTCAGAGGC CTCAATTACT ATTTGCCCAT GGTGGAGGAA 
GGGGAACCCG AACCCAAGTT TGAGAAATTC CTAGATGATG TGGATCCAGG AAGGAAGGGC 
TACATCTCAC TGGAGGAATA TACCTCATTT CTGATTGACA AGGAGTCCGA GAACATAAAG 
TCCAGTGAAG AAATTGAAAA TGGCTTCCAG GCCCTGGCTG AGGGAAAGCC TTACATCACA 
AAGGATGACC TGAAACAGGC CCTCACTCCA GAGCAGGTAC AATTCTGTGC CTCTCGAATG 
CAGCCCTACA CAGATCCTCG TNNGGTCGTA CTGCTGGCTA TGACTATGTT GGATTCACAA 
ATTCATACTT TGGTAACTGA TGAGGAGATG TTAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007481734.2
CDS	275..7513
Translation	MESTGIKVLETAEEIQGRRREVLSRYQRFKQLVTERGQKLEDSYHYQVFRRDADDLEKWILEKLKIASDENYKDPTNIQGKYQKHQSFEAEVQAKSRVIPELEEIRRVRFAEGHFAHEDTKEHLEELKKLWDLLLELTKEKGTRLLQVLKLQQYLQECADILEWISDKETIVTSVELGEDWERAEVLHKKFEEFQVDLAAREGRVAAVNEFANQCAQEDHSQLELIKTKQDEVNTNWQRLQGLALQRRETLSDAADLQRFNRDVAEAIQWIKEKEPLLTSEDFGKDLVSSEALFHSHKGLERSLAVMEDKVRELCAKADKLQVSHPSDAREIQEKKEDLSSNWALIRALATKRYKKLQASYWYQRFLSDFDELTGWMGEKTALINADELPTDVAGGEALLDRHQQHKHEIDSYEDRFQSAAETGQYLLDADHEASEEVCEKMTALDNDHAALLELWERRRHQYEQCLDLHLFYRDSEQVDSWMSRQEAFLENEDLGNSLGTVEALLQKHEDFEEAFGAQEEKITTLDKTATKLIDNDHYDSENIAGIRDGLLARRDALRDRAASRRGKLKDALLLQQLYQDSDDLKNWINKKKKLADDEDYKDSQNLKSRVQKQQVFQEELMANEVRLNNLKKTGQEMIDGNHYASVNVTARINEVTALWKELLETTEQKGSQLHEANQKLLFEQNVEDLARWLEEVEGQVASEDYGKGLADVQNLLRKHGLLETSVNAHQDQVDTITDLAAYFEEVGHPDAEEMKLRQESLVSSYESLKEPMDKRKRKLIDLLLLQQICRDTEDEEAWIQETEPSAASTYLGKDLIAAQNLLNRHQVILADIDSHEPRIHAITERGNKLVDEGHFAAEDVASRVKSMNENMESLQARAAQRQCDLEDNVHFQQYLADLHEAETWIQEKEPIVDNTNYGDDEEAAGALLKKHEAFLVDLNAFGTSMKALRKQADACRQQAAPMEGAAQEERVIALYDFEARSPREVTMKKDDVLTLLSSINKDWWKVEADDHQGFVPAVYVRRLARDELPRPERRRREEPSDIAQRQEQIDNQYHSLLERAEDRRRRLLQRYKEFLLAYEAGDMLDWIREKKEENTGVELEDVHELQKKFDDFQTDLKTNEPRLQDINKVADDLRLEDLLTPEGAEIQQELNSRWDSLQRLAEEQRLLLGSAHAVQMFHRDAEDTKERIEKKHQALSAADPGSDLFSVQALQRRHDAFERDLEPLGEKVTTLGETAERLIETHPDATEDLRRQQVELDGAWDNLLRRTEERKDDLSEAQKLYLFLNQAREILSWINSIRGPVSSVELAEDLTGTEILLERHQEHHDEMKAEAPTFQALEDFGIDLLSSGHRAGPEIEGKLQLVRLERDDLEKSWERRKKMLDQCLDFQLFQGDCEQAESWMVARESSLRTDDKSSLSSLEALMKKRDDLDKAINAQEEKINSLKSFAERLIDDDHYAKKEIADRLQRVLDRWNALKGQMIAEREQLSDYADLKQFYRDIEDLSDWINEMMPTACDESYKDPTNIQRKYLKHQTFENEVFGRNDEVENVIKLGNSLIDRRACDGQEEDVKERVSRLSEHWNQLLEKTKDKGQKLNEASRQQRFNTGIRDFEFWLSEAETLLAMKDQARDLASAANLLKKHQLLETEMSAREDFLKDLNELATHLIDSGAFNADEIKEKRDNINNRFRNVQDLAAAHHKKLKEAHALFQFFQDLDDEESWIKAKLLRVSSKDYGRDLPGVQNLQRKHKRLEGELDAHEPAIQGVLETAEKLKDSDAVGREEIQERLAQLVQHWNELKELTKIRGLRLDESQQYLQFIENVEEEEAWINEKTALVARGDTGDILPATQSLLKKHEALENDFANHEIKVQEICIEGEDLLGKEDTQHKDKITAKINGLKSKIPALAKAMAAWKAQLDEDAAFQLFNWKADTVESWIAEKESSLKTNGNGGDLAAYRMLLTKQDTLDDGLKSFEHKRLSEITDLKDQLVAGEHSRASTIEDRHAALLERWDQLLAAAAARRAKLLEEQLPLQKAEELFLEFAQKASAFNNWCENAEEDLSEPVHCVSLEEIQQLQKDHEAFLVSLQGARADFDHLVELDQQIKALNMPSSPYTWLTVEVLEGIWLNLPNIIKEREQELQKEEERQMKNRELCQEFEQEASAFSQWIQETRAYFLDGSLLKETGTLESQLEANKRKQKEIQVMKRQLNKIEDLGDIMEDALILDIKYSTIGLAQQWDQLHQLGIRMQHNLEQQIQAKDMIGVSEETLKEFSTTYQHFDETLSGRLSHKEFRSCLRGLNYYLPMVEEGEPEPKFEKFLDDVDPGRKGYISLEEYTSFLIDKESENIKSSEEIENGFQALAEGKPYITKDDLKQALTPEQVQFCASRMQPYTDPRnullVVLLAMTMLDSQIHTLVTDEEMLM

Target ORF information:

RefSeq Version	XM_007481672.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica spectrin alpha, erythrocytic 1 (SPTA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007481672.2

ORF Insert Sequence:

ATGGAGAGCA CTGGAATTAA AGTGCTGGAA ACAGCAGAAG AAATCCAGGG AAGACGACGA 
GAAGTGTTGA GTCGGTACCA GCGTTTCAAA CAGCTTGTTA CTGAACGTGG TCAAAAGCTT 
GAAGATTCCT ATCACTATCA GGTTTTCAGG CGAGATGCAG ATGACCTGGA GAAATGGATC 
TTGGAGAAAC TCAAGATTGC TTCTGATGAA AATTACAAAG ACCCAACTAA CATACAGGGG 
AAATATCAGA AACATCAATC CTTTGAAGCT GAAGTACAAG CAAAATCTAG AGTGATTCCA 
GAGCTGGAAG AAATCAGACG AGTTCGATTT GCTGAAGGAC ATTTTGCTCA TGAAGACACC 
AAGGAGCATC TGGAAGAACT GAAGAAACTG TGGGATTTGC TTTTAGAGCT GACTAAGGAG 
AAGGGCACTC GGTTGTTGCA GGTCCTGAAG CTGCAGCAGT ACTTGCAGGA GTGCGCTGAC 
ATCCTTGAGT GGATCAGTGA CAAAGAAACC ATTGTGACAT CAGTAGAACT GGGTGAAGAC 
TGGGAGAGAG CTGAAGTTCT GCACAAGAAA TTTGAGGAGT TCCAGGTGGA TCTGGCAGCT 
CGTGAGGGGC GAGTGGCTGC AGTGAATGAG TTTGCCAACC AGTGTGCCCA GGAAGATCAT 
TCGCAACTGG AACTAATCAA GACAAAGCAG GATGAAGTAA ATACCAATTG GCAGCGTCTC 
CAGGGCTTAG CTCTCCAGAG ACGAGAAACT TTGTCTGATG CTGCTGACTT GCAACGCTTC 
AACAGGGATG TAGCTGAAGC TATTCAGTGG ATCAAGGAGA AGGAGCCTCT TCTTACCTCG 
GAAGACTTTG GCAAAGATCT GGTTAGCTCT GAAGCCCTGT TCCACAGTCA CAAAGGGCTG 
GAAAGGAGCC TAGCTGTCAT GGAAGACAAG GTAAGAGAGC TGTGTGCTAA GGCAGATAAG 
CTACAGGTCT CCCATCCCTC AGATGCACGT GAGATCCAGG AGAAGAAAGA AGACCTCTCC 
TCTAACTGGG CACTAATCCG GGCTCTAGCC ACCAAAAGAT ATAAGAAGCT TCAGGCTTCA 
TACTGGTACC AACGCTTCCT CTCCGACTTT GATGAACTCA CAGGCTGGAT GGGAGAGAAA 
ACAGCCCTTA TCAATGCTGA TGAGCTGCCA ACAGATGTGG CAGGTGGGGA AGCCCTGTTA 
GACAGGCACC AACAGCATAA GCATGAAATT GATTCCTATG AAGACCGATT TCAGTCAGCT 
GCAGAAACTG GCCAGTATCT GTTGGATGCT GACCATGAGG CATCTGAAGA AGTTTGTGAA 
AAGATGACTG CTCTTGACAA CGATCACGCT GCACTGCTGG AATTATGGGA GAGACGTCGT 
CATCAATACG AGCAGTGCCT GGACCTACAC CTCTTCTATC GAGATAGTGA GCAAGTGGAC 
AGCTGGATGA GCAGACAGGA GGCATTCTTA GAGAATGAGG ACTTGGGGAA CTCTCTGGGC 
ACTGTTGAAG CCCTTCTTCA GAAGCATGAG GACTTTGAGG AGGCCTTTGG TGCCCAGGAA 
GAGAAAATTA CAACACTGGA CAAGACAGCA ACCAAACTGA TCGACAATGA CCATTATGAT 
TCAGAGAACA TCGCTGGGAT CAGGGATGGG CTTTTGGCAA GGCGAGATGC CCTCCGTGAC 
CGTGCTGCTT CCAGGCGAGG GAAGCTGAAA GATGCCCTGC TTCTGCAGCA GCTCTACCAA 
GACTCAGATG ACCTAAAGAA CTGGATCAAT AAGAAGAAAA AATTAGCAGA TGATGAGGAT 
TACAAGGACT CACAGAACCT AAAGAGCCGA GTTCAAAAGC AACAAGTATT TCAAGAGGAG 
CTGATGGCTA ATGAGGTTCG CCTCAACAAC CTGAAAAAAA CTGGCCAGGA GATGATTGAT 
GGCAACCACT ATGCCTCTGT TAATGTGACT GCCCGAATTA ATGAGGTCAC TGCCCTCTGG 
AAGGAGCTCC TAGAAACAAC AGAGCAGAAA GGATCCCAGC TGCATGAAGC CAATCAGAAG 
CTACTATTTG AACAGAATGT GGAGGATTTA GCCCGCTGGC TGGAGGAAGT GGAGGGTCAG 
GTTGCCTCTG AAGATTATGG GAAAGGCCTG GCTGATGTCC AGAATCTCCT TCGGAAACAT 
GGACTCTTAG AGACCAGTGT GAATGCTCAT CAGGACCAAG TAGACACAAT TACAGACCTA 
GCTGCCTATT TTGAGGAGGT GGGACATCCT GATGCTGAAG AAATGAAATT AAGGCAGGAA 
TCTCTGGTGT CAAGTTATGA GTCCCTGAAG GAACCAATGG ACAAAAGGAA GAGGAAGCTC 
ATTGACCTTC TGCTACTGCA ACAGATCTGC CGGGACACAG AAGATGAGGA GGCTTGGATT 
CAAGAGACAG AACCTTCTGC TGCCTCCACT TACCTTGGAA AGGACCTGAT TGCTGCTCAG 
AACCTGCTGA ATAGGCACCA GGTCATTTTG GCTGATATTG ACAGCCATGA ACCCCGAATT 
CATGCCATCA CAGAGAGAGG CAACAAATTG GTAGATGAAG GGCACTTTGC TGCAGAGGAT 
GTAGCCTCTA GAGTAAAGAG CATGAATGAG AACATGGAGT CCCTTCAGGC TCGGGCTGCC 
CAGCGACAGT GTGACCTGGA GGACAATGTC CATTTCCAGC AGTACCTTGC AGATCTACAT 
GAAGCAGAAA CCTGGATTCA GGAGAAGGAG CCCATTGTGG ACAACACAAA CTATGGGGAT 
GATGAGGAAG CTGCTGGGGC TCTTCTGAAG AAGCACGAAG CTTTCCTAGT GGACCTTAAT 
GCCTTTGGAA CCAGCATGAA GGCTCTGAGG AAGCAGGCAG ATGCATGCCG GCAACAAGCT 
GCACCTATGG AAGGGGCTGC GCAGGAAGAA CGGGTCATAG CTTTATATGA CTTCGAGGCC 
AGGAGCCCCA GAGAAGTCAC CATGAAGAAA GATGATGTCT TAACCCTGCT CAGCTCTATC 
AACAAGGACT GGTGGAAGGT AGAAGCTGAT GATCATCAGG GCTTTGTCCC AGCTGTGTAT 
GTCAGGAGAT TGGCCAGGGA TGAGCTCCCC AGGCCTGAGA GGCGGCGTCG AGAAGAGCCA 
AGTGACATTG CCCAACGCCA GGAGCAAATT GATAATCAGT ACCACTCTCT TCTGGAGAGG 
GCAGAAGACC GTCGGCGTCG CCTTTTACAG CGATACAAGG AGTTCCTGCT AGCTTATGAG 
GCTGGAGACA TGCTGGATTG GATCAGAGAG AAGAAGGAAG AAAACACTGG AGTAGAACTA 
GAAGATGTCC ATGAGCTGCA AAAAAAGTTT GATGACTTCC AGACGGATCT GAAGACCAAT 
GAACCTCGAC TTCAGGACAT TAACAAAGTG GCTGATGATC TTCGTTTAGA AGACCTTTTA 
ACTCCAGAAG GTGCTGAAAT CCAGCAGGAG TTAAATAGCC GCTGGGATTC CCTCCAGAGA 
CTTGCAGAGG AGCAGCGCCT GCTGCTGGGC AGTGCTCATG CTGTCCAAAT GTTCCACAGA 
GATGCAGAGG ACACCAAGGA ACGCATTGAG AAGAAGCATC AGGCCTTGAG TGCTGCAGAC 
CCTGGCTCAG ATCTGTTCAG TGTTCAGGCG CTTCAGCGTC GCCATGATGC TTTTGAGAGG 
GATTTGGAAC CTCTTGGAGA AAAGGTGACT ACTTTGGGAG AGACAGCAGA GAGGCTCATT 
GAGACTCACC CAGATGCCAC TGAGGACCTG CGCAGGCAAC AGGTTGAGCT TGACGGGGCC 
TGGGATAATT TGCTTCGGCG CACAGAAGAA AGAAAGGATG ACCTGAGCGA GGCGCAGAAG 
CTCTATTTGT TCCTCAATCA GGCCAGGGAA ATATTGTCGT GGATCAATAG CATTCGTGGC 
CCTGTGTCAT CAGTGGAATT GGCTGAGGAC CTCACAGGAA CTGAAATCTT ACTTGAAAGA 
CATCAGGAAC ACCATGATGA GATGAAGGCT GAAGCTCCTA CATTCCAGGC CCTAGAAGAC 
TTTGGTATCG ACCTTCTAAG CAGTGGCCAC CGGGCTGGCC CTGAGATAGA AGGGAAGTTG 
CAATTGGTGA GACTTGAAAG AGATGATTTG GAGAAATCTT GGGAGCGGCG AAAGAAGATG 
CTTGATCAAT GTCTGGATTT CCAGCTGTTC CAAGGAGATT GTGAGCAAGC AGAGAGCTGG 
ATGGTGGCCC GAGAGAGTTC TCTGAGGACA GATGACAAAA GCTCACTGAG TAGTCTGGAG 
GCCTTGATGA AGAAGCGTGA TGATTTAGAT AAGGCAATCA ATGCCCAGGA AGAAAAGATA 
AATTCACTGA AAAGTTTTGC AGAGCGGCTC ATAGATGATG ACCACTATGC CAAGAAAGAA 
ATTGCTGACC GGCTTCAGAG AGTGTTGGAC AGGTGGAATG CTCTCAAAGG ACAGATGATT 
GCAGAAAGAG AACAGCTTAG TGATTATGCA GACCTGAAAC AATTCTACCG TGACATTGAA 
GATTTGTCAG ATTGGATCAA TGAGATGATG CCAACAGCCT GTGATGAGTC CTACAAAGAT 
CCCACTAATA TCCAGAGAAA ATACTTGAAG CATCAGACAT TTGAAAATGA AGTCTTCGGC 
CGGAATGATG AAGTCGAAAA TGTCATCAAA CTGGGGAACT CCCTTATTGA TCGCAGAGCA 
TGTGATGGCC AGGAGGAAGA TGTGAAGGAA CGAGTGTCTA GACTTAGTGA ACACTGGAAC 
CAGCTCCTGG AGAAAACCAA AGACAAAGGG CAGAAATTGA ACGAGGCAAG CCGGCAACAG 
AGGTTCAACA CAGGCATCCG TGACTTTGAA TTCTGGCTCT CTGAGGCAGA GACACTACTG 
GCGATGAAAG ATCAAGCCAG GGACTTGGCT TCAGCAGCCA ACTTACTTAA GAAGCATCAA 
CTGTTGGAAA CAGAAATGTC AGCTCGTGAG GACTTTCTCA AAGATCTGAA TGAACTGGCT 
ACTCACTTAA TCGACAGTGG GGCTTTCAAT GCTGATGAGA TCAAGGAGAA ACGGGATAAT 
ATCAATAACC GATTCCGGAA TGTCCAGGAC CTGGCTGCTG CGCATCACAA AAAGCTCAAG 
GAGGCCCATG CTCTTTTCCA GTTTTTCCAG GATCTGGATG ATGAGGAATC CTGGATTAAG 
GCAAAGCTTC TACGAGTGAG CTCCAAGGAC TATGGACGGG ATCTTCCTGG TGTTCAGAAC 
CTACAAAGGA AACATAAACG CCTGGAAGGG GAGCTGGATG CCCATGAACC AGCTATCCAG 
GGAGTGTTGG AGACTGCAGA AAAACTGAAA GACAGTGATG CTGTGGGGCG AGAGGAGATC 
CAAGAGAGGC TGGCTCAGCT GGTGCAGCAT TGGAATGAGC TGAAGGAGTT GACCAAGATT 
CGTGGGCTCC GACTAGATGA GTCCCAACAG TATCTTCAGT TCATTGAGAA TGTTGAGGAA 
GAAGAGGCCT GGATCAATGA GAAGACAGCT CTTGTTGCCC GAGGGGATAC AGGAGACATA 
TTACCTGCCA CACAGAGCTT ACTTAAGAAG CATGAAGCTT TGGAGAATGA TTTTGCAAAC 
CATGAGATTA AAGTTCAGGA AATATGCATT GAAGGAGAAG ACCTTCTGGG CAAGGAGGAC 
ACACAGCACA AAGACAAAAT CACAGCCAAG ATCAATGGTC TGAAGTCAAA GATCCCTGCT 
TTAGCCAAAG CAATGGCAGC CTGGAAGGCC CAGTTGGACG AGGATGCTGC TTTCCAATTG 
TTTAACTGGA AGGCTGATAC AGTAGAGTCC TGGATAGCTG AAAAAGAATC TTCCCTGAAG 
ACCAATGGTA ATGGAGGAGA CCTTGCTGCT TACCGTATGC TACTCACTAA GCAGGATACA 
CTGGATGATG GCCTGAAAAG CTTTGAGCAC AAACGGCTGT CAGAAATAAC AGACTTAAAA 
GACCAACTGG TGGCAGGGGA ACACAGCAGG GCAAGTACCA TCGAGGACCG GCATGCCGCC 
CTTCTCGAAC GCTGGGACCA GCTACTGGCT GCTGCTGCAG CCCGAAGGGC AAAGCTGCTG 
GAGGAGCAGC TCCCCCTTCA GAAGGCTGAA GAGTTATTCT TGGAGTTTGC CCAGAAGGCT 
TCAGCCTTCA ACAACTGGTG TGAGAATGCA GAAGAGGACC TCTCTGAACC TGTACACTGT 
GTCTCCTTAG AAGAAATCCA ACAACTTCAG AAGGACCATG AGGCCTTTCT GGTCTCCCTC 
CAGGGAGCTC GAGCTGACTT TGACCACTTG GTAGAGCTGG ATCAGCAAAT TAAGGCTTTG 
AACATGCCCT CCAGTCCCTA TACCTGGTTA ACCGTGGAAG TTCTAGAAGG CATCTGGTTG 
AATCTGCCCA ATATCATCAA GGAAAGAGAG CAGGAATTGC AAAAAGAGGA AGAGAGACAA 
ATGAAGAATC GTGAGCTCTG CCAAGAGTTT GAACAAGAGG CCAGTGCATT CTCCCAGTGG 
ATCCAGGAAA CCAGAGCTTA TTTCCTAGAT GGATCATTGC TCAAGGAAAC TGGAACCCTA 
GAATCCCAGC TGGAAGCTAA TAAACGGAAG CAGAAAGAAA TTCAAGTGAT GAAGCGTCAG 
TTGAACAAGA TTGAGGACCT GGGAGATATC ATGGAGGATG CTCTGATCTT GGACATCAAA 
TACAGCACCA TTGGATTAGC CCAGCAGTGG GATCAGCTCC ACCAGCTAGG AATAAGAATG 
CAACACAATC TAGAGCAACA GATCCAGGCC AAGGACATGA TTGGTGTGAG TGAAGAGACG 
CTAAAGGAAT TTAGCACAAC CTATCAACAT TTTGATGAGA CCTTGTCTGG AAGGCTGAGT 
CACAAGGAAT TCCGGTCCTG TCTCAGAGGC CTCAATTACT ATTTGCCCAT GGTGGAGGAA 
GGGGAACCCG AACCCAAGTT TGAGAAATTC CTAGATGATG TGGATCCAGG AAGGAAGGGC 
TACATCTCAC TGGAGGAATA TACCTCATTT CTGATTGACA AGGAGTCCGA GAACATAAAG 
TCCAGTGAAG AAATTGAAAA TGGCTTCCAG GCCCTGGCTG AGGGAAAGCC TTACATCACA 
AAGGATGACC TGAAACAGGC CCTCACTCCA GAGCAGGTAC AATTCTGTGC CTCTCGAATG 
CAGCCCTACA CAGATCCTCG TNNGGTCGTA CTGCTGGCTA TGACTATGTT GGATTCACAA 
ATTCATACTT TGGTAACTGA TGAGGAGATG TTAATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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SPTA1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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