MYOF cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYOF
Entrez Gene ID	100023814
Full Name	myoferlin
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016426160.1	XP_016281646.1	myoferlin isoform X5
XM_016426154.1	XP_016281640.1	myoferlin isoform X1
XM_016426161.1	XP_016281647.1	myoferlin isoform X6
XM_007478795.2	XP_007478857.2	myoferlin isoform X4
XM_001375213.3	XP_001375250.1	myoferlin isoform X7
XM_007478794.2	XP_007478856.2	myoferlin isoform X2
XM_007478796.2	XP_007478858.1	myoferlin isoform X8
XM_016426157.1	XP_016281643.1	myoferlin isoform X3

Pathways from BioSystems
Gene Ontology

The following MYOF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYOF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc00887	XM_001375213.3 Latest version!	Monodelphis domestica myoferlin (MYOF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc00888	XM_007478796.2 Latest version!	Monodelphis domestica myoferlin (MYOF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc38444	XM_016426154.1 Latest version!	Monodelphis domestica myoferlin (MYOF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc38445	XM_007478794.2 Latest version!	Monodelphis domestica myoferlin (MYOF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc38446	XM_016426157.1 Latest version!	Monodelphis domestica myoferlin (MYOF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc38447	XM_007478795.2 Latest version!	Monodelphis domestica myoferlin (MYOF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc38448	XM_016426160.1 Latest version!	Monodelphis domestica myoferlin (MYOF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc38449	XM_016426161.1 Latest version!	Monodelphis domestica myoferlin (MYOF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYOF ( XM_001375213.3 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_001375250.1 Monodelphis domestica myoferlin (MYOF), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMc00887
Clone ID Related Accession (Same CDS sequence)	XM_001375213.3
Accession Version	XM_001375213.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	myoferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_001375213.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGGG TCATTGTGGA GTCTGCGAGT GATATCCCTA AAGGGAAATT TGGAAAACCG 
GATCCAATTG TCTCAGTTAT TTTTAAGGAT GAGAAGAAGA AAACGAAGAA AATTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATATTT GAATTTGATT TGAAGGGCAT ACCATTGGAC 
TTTTCATCTA CTCTTGGCAT CACTGTGAAA GATTTCGAGA CAATTGGACA AAATAAACTG 
ATTGGCGTTG CAACAATATC ACTGAAGGAC TTGATAGGTG GCCAGAGTCG ATCTCTTCCT 
TATAAACTCA TATCTTTGGT AAATGAAAAG GGTCAAGATA CTGGGGCTAC AATCGATCTG 
GTGGTTGCCT ATGATCCACC AGCAGCCATG GCAACAAATC CAAATGAACT AAGTGGGATC 
ACCATAGAAG GCACGGGAGA AGAAGAGGAA GAAGACACAG GAGATGAAGA TACTGCATTC 
AAAGTCATTG CCCCAAGGGT TCCAGCAACA AATATTTCTG AAGCCCAGCT GGCCAGGAGG 
ATTACAAAAG GAAAGAATAC CCGAAAGATC CTTTCCAATA AGCCACAAGA CTTCCAGATT 
CGTGTTAGAG TGATTGAAGG GCGCCAATTA AGTGGGAATA ACATCAAACC AGTGGTCAAA 
GTCCATGTCT GTGGTCAGAC ACATCGAACA AGAATAAAAA GAGGGAATAA TCCATTTTTT 
GACGAATTAT TTTTCTACAA TGTCCACATG ACCCCTCTTG AACTGGTTGA TGAGACAATA 
AGCATTCGGG TATATGATTC CCATTCTTTG AGAGCAGACT GTCTGATGGG AGAATTTAAG 
ATTGATATTG GTTACATTTA TGATGAACCT GGTCATGCTG TCATGAGAAA ATGGCTTCTT 
CTAAATGACC CTGAAGATAT AAGTTCTGGT GCTAAAGGTT ACATGAAAGT CAGCATGTTT 
GTCCTTGGAA CAGGAGATGA ACCACCAACA GAGAAGAGAG ATCGAGATAA TGACAGTGAT 
GATGTGGAGA GTAATTTGTT ACTTCCTGCT GGAATTGCCA TGCGGTGGGT CACTTTCCTC 
CTGAAAATTT ACAGAGCTGA GGATATTCCT CAGATGGATG ATGCCTTTTC ACAGACTGTC 
AAAGAAATAT TTGGAGCCAA TGTAGACAAG AAAAACTTAG TGGATCCATT TGTAGAAGTT 
TGTTTTGCTG GGAAAAAGGT TTCCACAGGC ATAATAGAGA AAAATGCAAA TCCAGAGTGG 
AATCAAGTTG TCAATCTTCA AATCAAGTTT CCTTCAATGT GTGAAAAAAT AAAGTTAACA 
GTATATGACT GGGACCGCCT TACCAAGAAT GATGTAGTAG GGACAACCTA TCTGTATCTT 
TCTAAAATTG CTGCATCGGG TGGGGAAGTT GAAGATTCCT CACCTTCGGG AGCTTGGGCT 
GCATCATCCA CAGAGAAAAC AGGAGAAACA GAAGTGGGCT TTGTACCAAC ATTTGGACCT 
TGTTATCTAA ATCTTTATGG AAGTCCAAGA GAATACACTG GATTCCCAGA TCCATATGAT 
GACTTGAATT CTGGAAAGGG TGAAGGAGTT GCCTACAGAG GTCGAATCTT GGTAGAACTG 
AATACTTTAC TTGAGAAAAC ACCCCCTGAC AAGAAGGTTG AAACCATATC CAATGATGAT 
TTGCTGGTTG TTGAGAAATA TCAGCGTAGG CGAAAATATA GCCTGTCTGC GGTATTTCAT 
TCTGCAACCA TGCTGCAAGA TATTGGAGAG GCCATTCAGT TTGAAGTCAG CATTGGAAAT 
TACGGCAATA AGTTTGATGC TACCTGTAAG CCTTTGGCAT CGACAACTCA ATACAGTCGG 
GCTGTGTTTG ATGGAAATTT CTATTATTAT TTGCCATGGG CCCAGAAGAA GCCAGTGGTG 
ACTCTAACTT CTTATTGGGA AGACATTAGT CACAGACTGG ATGCAGTGAA CACCATTCTT 
GTTATGGCAG AACGGCTGCA CGTGAACATT GAGAATTTAA AATCAGGAAT ACAAGCCAAA 
ATGTCTGGGA ATCATTTGGC TGAAATGTGG CTGAAGTTGA TTGATGAACT TATCGAAGAT 
ACAAGCATGC CATTGCCTTC ACTAGAAGGG AAATCTAATG TCACCATTCT AGACACTCAG 
ATCCAAAAGC TGCGTCTTCG ATCCCTTATC CAGATTCAAG AAGCAGCTAG AAAAATGAGG 
TCTGAAGCCA AACATGTGAA GTCTACTTTG ACAGAAATTG AAGATTGGTT TGATAAGCTA 
ATGCAACTTT CTGAAGAGCC ACAAAACAGT ATGCCCGATA TTATCATATG GATGATCCGA 
GGAGAGAAAA GATTGGCCTA TGCTAGAATT CCTGCACACC AGGTTTTATA TTCTACTACC 
AGTGAAGAAG CTTCTGGAAA GTACTGTGGG AAGACCCAGA CCATCTTTTT GAAGTACCCA 
CAGGACAGGA GCTATGGTGT GAAGGTTCCA GCAGAATTGC GGGTTAACAT TTGGCTTGGA 
TTAAGTGCTG TTGAAAAGAA GTTTAACAGC TTTGCTGAAG GAACATTCAC TGTCTTTGCA 
GAAATGTATG AAAATCAAGC TTTGATGTTT GGAAAATGGG GCACTTCTGG ACTTGTGGGT 
CGACATAAAT TTTCAGATGT CACAGGCAAG ATAAAGCTCA AGAAAGAGTT CTTTTTGCCC 
CCTAAAGGCT GGGAATGGGA AGGTGAATGG ATGGTGGATC CTGAAAGAAG TTTATTGACT 
GAAGCGGATG CTGGCCACAC ACACTTTACA GATGAAGTGT ATCAGAATGA AAGCCGCTAT 
CCAGGTGGAG AATGGAAGGC AATGGAGGAC AGCTACACAG ATGTGAATGG AGAAAAAGCA 
GCATCTCCTA ATGAGATGAC TTGCCCCCCT GGCTGGGAAT GGGAAGATGA TGCTTGGATG 
TATGATATAA ACCGAGCTGT AGATGAAAAA GGCTGGGAAT ATGGAATTAC TATTCCTCCT 
GATAACAAGC CAAAGTCCTG GGTTGCTGCA GAGAAGATGT ATCATACACA CAGGCGGCGG 
CGAATGGTCC GCAAACGCAA AAAGGATGTG ACACAGTCGG CTGCAAGTAC TGCAAGGGGG 
TGGGAAGCAC TAGAAGATAA AGAAGGCTGG GAATATGCAT CTTTAATTGG ATGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACTTTT CGTCGAAGAC GCTGGAGAAG AAAGATGGCT 
CCTTCTGAAA CACATGGTTC AGCTGCTATC TTTAAACTTG AAGGTGCCAT TGGAGCAGAC 
ACTACTGAAG ATGGAGATGA AAAGAGCACG GAGAAAAACA AACATAGTGC AACAACCGTG 
TTTGGTGCAA ACACTCCAAT TGTTTCCTGT AATTTTGACA GAGTATATAA TTATCATCTT 
CGCTGCTATA TATATCAAGC TCGAAATCTT ATGGCTTTAG ATAAAGATAG TTTTTCAGAT 
CCCTATGCTC ATGTTTCATT CCTCCATCGG AGCCAAACAA CTGAAATCAT CCACTCAACA 
CTGAATCCAA CCTGGGACCA AACTCTAATA TTTGATGACA TTGAAATTTT TGGAGAACCA 
CAAACAATCA TACAAAACCC ACCCAAAGTT GTCATTGAAC TCTTTGACAA TGACCAAGTG 
GGCAAGGATG AATTTTTAGG TCGAAGTTTT TGCTCCCCAG TTGTCAAGCT AAATCCAGAA 
GTTGACATCA CCCCTAAGCT TCTCTGGCAA CCAGTCATAA ATGGAGATAA GGCTTGTGGG 
GATGTACTTG TAGCTGCTGA GCTTATTCTC AAAGATAAGG TTGGCTCCAA TCTTCCAATT 
CTCCCATCTC AGAGGGCTCC AAATCTCTAC ATGGTTCCTC AAGGAATTAG GCCTGTGGTT 
CAACTCACTG CTATTGAGAT TTTAGCTTGG GGTTTAAGAA ACATGAAAAA CTACCAGATG 
GCTTCTGTGA CTTCCCCAAG TCTTATCATA GAGTGTGGAG GAGAAAGGAT AGAATCAGCA 
TTGATCAAAA ACCTCAAGAA GACACCCAAC TTTCCTAGTT CTGTTCTCTT CATGAAAGTG 
CTATTGCCCA AGGAAGAATT GTACACACCA CCATTGGTTA TCAAAGTGAT CGACCACAGA 
CAATTTGGGC GAAAGCCCAT CGTGGGTCAG TGCACCATCG ACCTTCTTGA ACAGTTTCGT 
TGTGACCCTT ATGCATTTAA GGAAGATATT GCACCCCAGG TAAAAGCTTC TATCTTGTCT 
GCCCCACTTA GCCGGGATGT GGTTATTGAA GTTGAGGACA CAAAACCATT GCTTCCAACC 
AAGTTAACTG AAAAGGAGGA AGAAGTTGTT GACTGGTGGA GCAAATTTTA TGCATCTGTT 
GGAGAACCTG AAAAGTGTGG GCAATATATC CAGAAGGAAT ATGCCAAACT AAAGGTATAT 
AATTGTGAAC TAGAGAGTGT TCCAGAGTTT AAGGGTCTGA CAGACTTTTC AGATACCTTT 
AAGCTTTACA GAGGGAAGTC TGATGAGATT GATGATCCTT CAGTGGTAGG AGAGTTCAAG 
GGCTCCTTTA GAATCTATCC ACTGCCTGAT GATCCAAATG TGCCAGCTCC TCCCAGGCAG 
TTTCGGGAAT TACCTGACAG TGTCCCACAG GAATGCATTG TGAGGATTTA TATTGTTCGG 
GGCATAGATC TCCAGCCTCA GGATAACAAT GGCCTGTGTG ACCCTTATAT AAAAATATCC 
CTGAGTAAAA AAGTCATTGA AGATCGAGAT AATTACATTC CAAATACACT CAACCCAATT 
TTTGGAAGGA TGTATGAGCT AAGCTGCTTC TTACCTCAAG AAAAAGACCT GAAAATTTCT 
GTCTATGATT ATGACACACT TACTCGTGAT GAAAAAGTAG GAGAAACTAT CATTGATCTA 
GAAAATCGCT TCCTTTCCCG ATTTGGGTCG AATTGTGGCA TAGCAGAGCA ATATTGCATT 
TCTGGAGTCA ATACTTGGAG AGATCAACTA AAACCAACCC AATTGTTATG CAATGTAGCT 
AAGTTTAAAG GATTGCCACT CCCTATTCTT TCTGAAGATG GAAATAGGCT AAGATTTGGA 
GGTCGAGAGT ACACCTTGGA AGAATTTGAG GCCAACAGAA ATCTGCACCA GCACCTGGGA 
CCTGCAGAGG AGCGTCTTGC TCTGTATATC CTTCGGACTC AGGGTCAAGT CCCTGAGCAT 
GTGGAAACAA GGACTTTGTA CAGCACCTTC CAACCAAATA TTTCACAGGG AAAACTTCAG 
ATGTGGGTGG ATGTTTTCCC CAAAAGTTTG GGGCCACCAG GCCCTCCTTT CAATATCACT 
CCCCGGAAGG CCAAGAGGTA TGTCCTACGT GTGATCATTT GGAACACTAA AGATGTCATT 
CTTGATGAGA AAAGTATCAC AGGAGAAGAG ATGACTGACA TCTATGTGAA AGGCTGGATT 
CCTGGGAATG AAGAAAACAA GCAGAAGACA GATGTCCATT ACAGGTCATT GGATGGTGAA 
GGAAACTTTA ATTGGAGATT TGTTTTCCCT TTTGAATACC TGCCTGCTGA ACAACTCTGT 
ATTGTTTCTA AAAAAGAACA TTTCTGGAGT ATTGACCAGA CTGAATTTCG AGTTCCTCCC 
AGACTCATCA TTCAGATATG GGACAATGAT AAGTTTTCTT TAGATGACTA CTTGGGTTTT 
CTGGAACTTG ATTTACACAA CACAATTATC CCTGCAAAGT CTTCAGAAAA GTGCAAATTG 
GACATGATTT CAGAGTATAG GGCAGTGAAT CCTCTAAAGT CCAAGACAGC CTCACTTTTT 
GAACAGAAAT CGATGAAAGG ATGGTGGCCA TGCTATGCAG AAAAAGATGG CGCACGTGTG 
ATGACTGGTA AAGTGGAGAT GACTTTGGAA GTCCTCAATG AGAAGGAGGT TGATGAAAGA 
CCAGCTGGGA AAGGAAGGGA TGAGCCCAAC ATGAACCCCA AGCTAGAATT ACCAAATCGA 
CCAGAAACTT CATTCCTTTG GTTTACCAAC CCATGCAAGA CAATGAAATT CATCGTGTGG 
CGTCGATTTA AATGGGTCAT CATTGGCTTA CTCATCCTGC TGGTCTTGTT GCTGTTTTTG 
GCAGTACTCC TATATTCTCT GCCGAACTAT ATATCAATGA AGATCGTGAG ACCAAATGCA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001375250.1
CDS	179..6364
Translation	MLRVIVESASDIPKGKFGKPDPIVSVIFKDEKKKTKKIDNELNPVWNEIFEFDLKGIPLDFSSTLGITVKDFETIGQNKLIGVATISLKDLIGGQSRSLPYKLISLVNEKGQDTGATIDLVVAYDPPAAMATNPNELSGITIEGTGEEEEEDTGDEDTAFKVIAPRVPATNISEAQLARRITKGKNTRKILSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHMTPLELVDETISIRVYDSHSLRADCLMGEFKIDIGYIYDEPGHAVMRKWLLLNDPEDISSGAKGYMKVSMFVLGTGDEPPTEKRDRDNDSDDVESNLLLPAGIAMRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGANVDKKNLVDPFVEVCFAGKKVSTGIIEKNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEDSSPSGAWAASSTEKTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDDLNSGKGEGVAYRGRILVELNTLLEKTPPDKKVETISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNFYYYLPWAQKKPVVTLTSYWEDISHRLDAVNTILVMAERLHVNIENLKSGIQAKMSGNHLAEMWLKLIDELIEDTSMPLPSLEGKSNVTILDTQIQKLRLRSLIQIQEAARKMRSEAKHVKSTLTEIEDWFDKLMQLSEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTTSEEASGKYCGKTQTIFLKYPQDRSYGVKVPAELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKKEFFLPPKGWEWEGEWMVDPERSLLTEADAGHTHFTDEVYQNESRYPGGEWKAMEDSYTDVNGEKAASPNEMTCPPGWEWEDDAWMYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRMVRKRKKDVTQSAASTARGWEALEDKEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGSAAIFKLEGAIGADTTEDGDEKSTEKNKHSATTVFGANTPIVSCNFDRVYNYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSQTTEIIHSTLNPTWDQTLIFDDIEIFGEPQTIIQNPPKVVIELFDNDQVGKDEFLGRSFCSPVVKLNPEVDITPKLLWQPVINGDKACGDVLVAAELILKDKVGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIIECGGERIESALIKNLKKTPNFPSSVLFMKVLLPKEELYTPPLVIKVIDHRQFGRKPIVGQCTIDLLEQFRCDPYAFKEDIAPQVKASILSAPLSRDVVIEVEDTKPLLPTKLTEKEEEVVDWWSKFYASVGEPEKCGQYIQKEYAKLKVYNCELESVPEFKGLTDFSDTFKLYRGKSDEIDDPSVVGEFKGSFRIYPLPDDPNVPAPPRQFRELPDSVPQECIVRIYIVRGIDLQPQDNNGLCDPYIKISLSKKVIEDRDNYIPNTLNPIFGRMYELSCFLPQEKDLKISVYDYDTLTRDEKVGETIIDLENRFLSRFGSNCGIAEQYCISGVNTWRDQLKPTQLLCNVAKFKGLPLPILSEDGNRLRFGGREYTLEEFEANRNLHQHLGPAEERLALYILRTQGQVPEHVETRTLYSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKRYVLRVIIWNTKDVILDEKSITGEEMTDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFEYLPAEQLCIVSKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHNTIIPAKSSEKCKLDMISEYRAVNPLKSKTASLFEQKSMKGWWPCYAEKDGARVMTGKVEMTLEVLNEKEVDERPAGKGRDEPNMNPKLELPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLILLVLLLFLAVLLYSLPNYISMKIVRPNAK

Target ORF information:

RefSeq Version	XM_001375213.3
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica myoferlin (MYOF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001375213.3

ORF Insert Sequence:

ATGCTTCGGG TCATTGTGGA GTCTGCGAGT GATATCCCTA AAGGGAAATT TGGAAAACCG 
GATCCAATTG TCTCAGTTAT TTTTAAGGAT GAGAAGAAGA AAACGAAGAA AATTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATATTT GAATTTGATT TGAAGGGCAT ACCATTGGAC 
TTTTCATCTA CTCTTGGCAT CACTGTGAAA GATTTCGAGA CAATTGGACA AAATAAACTG 
ATTGGCGTTG CAACAATATC ACTGAAGGAC TTGATAGGTG GCCAGAGTCG ATCTCTTCCT 
TATAAACTCA TATCTTTGGT AAATGAAAAG GGTCAAGATA CTGGGGCTAC AATCGATCTG 
GTGGTTGCCT ATGATCCACC AGCAGCCATG GCAACAAATC CAAATGAACT AAGTGGGATC 
ACCATAGAAG GCACGGGAGA AGAAGAGGAA GAAGACACAG GAGATGAAGA TACTGCATTC 
AAAGTCATTG CCCCAAGGGT TCCAGCAACA AATATTTCTG AAGCCCAGCT GGCCAGGAGG 
ATTACAAAAG GAAAGAATAC CCGAAAGATC CTTTCCAATA AGCCACAAGA CTTCCAGATT 
CGTGTTAGAG TGATTGAAGG GCGCCAATTA AGTGGGAATA ACATCAAACC AGTGGTCAAA 
GTCCATGTCT GTGGTCAGAC ACATCGAACA AGAATAAAAA GAGGGAATAA TCCATTTTTT 
GACGAATTAT TTTTCTACAA TGTCCACATG ACCCCTCTTG AACTGGTTGA TGAGACAATA 
AGCATTCGGG TATATGATTC CCATTCTTTG AGAGCAGACT GTCTGATGGG AGAATTTAAG 
ATTGATATTG GTTACATTTA TGATGAACCT GGTCATGCTG TCATGAGAAA ATGGCTTCTT 
CTAAATGACC CTGAAGATAT AAGTTCTGGT GCTAAAGGTT ACATGAAAGT CAGCATGTTT 
GTCCTTGGAA CAGGAGATGA ACCACCAACA GAGAAGAGAG ATCGAGATAA TGACAGTGAT 
GATGTGGAGA GTAATTTGTT ACTTCCTGCT GGAATTGCCA TGCGGTGGGT CACTTTCCTC 
CTGAAAATTT ACAGAGCTGA GGATATTCCT CAGATGGATG ATGCCTTTTC ACAGACTGTC 
AAAGAAATAT TTGGAGCCAA TGTAGACAAG AAAAACTTAG TGGATCCATT TGTAGAAGTT 
TGTTTTGCTG GGAAAAAGGT TTCCACAGGC ATAATAGAGA AAAATGCAAA TCCAGAGTGG 
AATCAAGTTG TCAATCTTCA AATCAAGTTT CCTTCAATGT GTGAAAAAAT AAAGTTAACA 
GTATATGACT GGGACCGCCT TACCAAGAAT GATGTAGTAG GGACAACCTA TCTGTATCTT 
TCTAAAATTG CTGCATCGGG TGGGGAAGTT GAAGATTCCT CACCTTCGGG AGCTTGGGCT 
GCATCATCCA CAGAGAAAAC AGGAGAAACA GAAGTGGGCT TTGTACCAAC ATTTGGACCT 
TGTTATCTAA ATCTTTATGG AAGTCCAAGA GAATACACTG GATTCCCAGA TCCATATGAT 
GACTTGAATT CTGGAAAGGG TGAAGGAGTT GCCTACAGAG GTCGAATCTT GGTAGAACTG 
AATACTTTAC TTGAGAAAAC ACCCCCTGAC AAGAAGGTTG AAACCATATC CAATGATGAT 
TTGCTGGTTG TTGAGAAATA TCAGCGTAGG CGAAAATATA GCCTGTCTGC GGTATTTCAT 
TCTGCAACCA TGCTGCAAGA TATTGGAGAG GCCATTCAGT TTGAAGTCAG CATTGGAAAT 
TACGGCAATA AGTTTGATGC TACCTGTAAG CCTTTGGCAT CGACAACTCA ATACAGTCGG 
GCTGTGTTTG ATGGAAATTT CTATTATTAT TTGCCATGGG CCCAGAAGAA GCCAGTGGTG 
ACTCTAACTT CTTATTGGGA AGACATTAGT CACAGACTGG ATGCAGTGAA CACCATTCTT 
GTTATGGCAG AACGGCTGCA CGTGAACATT GAGAATTTAA AATCAGGAAT ACAAGCCAAA 
ATGTCTGGGA ATCATTTGGC TGAAATGTGG CTGAAGTTGA TTGATGAACT TATCGAAGAT 
ACAAGCATGC CATTGCCTTC ACTAGAAGGG AAATCTAATG TCACCATTCT AGACACTCAG 
ATCCAAAAGC TGCGTCTTCG ATCCCTTATC CAGATTCAAG AAGCAGCTAG AAAAATGAGG 
TCTGAAGCCA AACATGTGAA GTCTACTTTG ACAGAAATTG AAGATTGGTT TGATAAGCTA 
ATGCAACTTT CTGAAGAGCC ACAAAACAGT ATGCCCGATA TTATCATATG GATGATCCGA 
GGAGAGAAAA GATTGGCCTA TGCTAGAATT CCTGCACACC AGGTTTTATA TTCTACTACC 
AGTGAAGAAG CTTCTGGAAA GTACTGTGGG AAGACCCAGA CCATCTTTTT GAAGTACCCA 
CAGGACAGGA GCTATGGTGT GAAGGTTCCA GCAGAATTGC GGGTTAACAT TTGGCTTGGA 
TTAAGTGCTG TTGAAAAGAA GTTTAACAGC TTTGCTGAAG GAACATTCAC TGTCTTTGCA 
GAAATGTATG AAAATCAAGC TTTGATGTTT GGAAAATGGG GCACTTCTGG ACTTGTGGGT 
CGACATAAAT TTTCAGATGT CACAGGCAAG ATAAAGCTCA AGAAAGAGTT CTTTTTGCCC 
CCTAAAGGCT GGGAATGGGA AGGTGAATGG ATGGTGGATC CTGAAAGAAG TTTATTGACT 
GAAGCGGATG CTGGCCACAC ACACTTTACA GATGAAGTGT ATCAGAATGA AAGCCGCTAT 
CCAGGTGGAG AATGGAAGGC AATGGAGGAC AGCTACACAG ATGTGAATGG AGAAAAAGCA 
GCATCTCCTA ATGAGATGAC TTGCCCCCCT GGCTGGGAAT GGGAAGATGA TGCTTGGATG 
TATGATATAA ACCGAGCTGT AGATGAAAAA GGCTGGGAAT ATGGAATTAC TATTCCTCCT 
GATAACAAGC CAAAGTCCTG GGTTGCTGCA GAGAAGATGT ATCATACACA CAGGCGGCGG 
CGAATGGTCC GCAAACGCAA AAAGGATGTG ACACAGTCGG CTGCAAGTAC TGCAAGGGGG 
TGGGAAGCAC TAGAAGATAA AGAAGGCTGG GAATATGCAT CTTTAATTGG ATGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACTTTT CGTCGAAGAC GCTGGAGAAG AAAGATGGCT 
CCTTCTGAAA CACATGGTTC AGCTGCTATC TTTAAACTTG AAGGTGCCAT TGGAGCAGAC 
ACTACTGAAG ATGGAGATGA AAAGAGCACG GAGAAAAACA AACATAGTGC AACAACCGTG 
TTTGGTGCAA ACACTCCAAT TGTTTCCTGT AATTTTGACA GAGTATATAA TTATCATCTT 
CGCTGCTATA TATATCAAGC TCGAAATCTT ATGGCTTTAG ATAAAGATAG TTTTTCAGAT 
CCCTATGCTC ATGTTTCATT CCTCCATCGG AGCCAAACAA CTGAAATCAT CCACTCAACA 
CTGAATCCAA CCTGGGACCA AACTCTAATA TTTGATGACA TTGAAATTTT TGGAGAACCA 
CAAACAATCA TACAAAACCC ACCCAAAGTT GTCATTGAAC TCTTTGACAA TGACCAAGTG 
GGCAAGGATG AATTTTTAGG TCGAAGTTTT TGCTCCCCAG TTGTCAAGCT AAATCCAGAA 
GTTGACATCA CCCCTAAGCT TCTCTGGCAA CCAGTCATAA ATGGAGATAA GGCTTGTGGG 
GATGTACTTG TAGCTGCTGA GCTTATTCTC AAAGATAAGG TTGGCTCCAA TCTTCCAATT 
CTCCCATCTC AGAGGGCTCC AAATCTCTAC ATGGTTCCTC AAGGAATTAG GCCTGTGGTT 
CAACTCACTG CTATTGAGAT TTTAGCTTGG GGTTTAAGAA ACATGAAAAA CTACCAGATG 
GCTTCTGTGA CTTCCCCAAG TCTTATCATA GAGTGTGGAG GAGAAAGGAT AGAATCAGCA 
TTGATCAAAA ACCTCAAGAA GACACCCAAC TTTCCTAGTT CTGTTCTCTT CATGAAAGTG 
CTATTGCCCA AGGAAGAATT GTACACACCA CCATTGGTTA TCAAAGTGAT CGACCACAGA 
CAATTTGGGC GAAAGCCCAT CGTGGGTCAG TGCACCATCG ACCTTCTTGA ACAGTTTCGT 
TGTGACCCTT ATGCATTTAA GGAAGATATT GCACCCCAGG TAAAAGCTTC TATCTTGTCT 
GCCCCACTTA GCCGGGATGT GGTTATTGAA GTTGAGGACA CAAAACCATT GCTTCCAACC 
AAGTTAACTG AAAAGGAGGA AGAAGTTGTT GACTGGTGGA GCAAATTTTA TGCATCTGTT 
GGAGAACCTG AAAAGTGTGG GCAATATATC CAGAAGGAAT ATGCCAAACT AAAGGTATAT 
AATTGTGAAC TAGAGAGTGT TCCAGAGTTT AAGGGTCTGA CAGACTTTTC AGATACCTTT 
AAGCTTTACA GAGGGAAGTC TGATGAGATT GATGATCCTT CAGTGGTAGG AGAGTTCAAG 
GGCTCCTTTA GAATCTATCC ACTGCCTGAT GATCCAAATG TGCCAGCTCC TCCCAGGCAG 
TTTCGGGAAT TACCTGACAG TGTCCCACAG GAATGCATTG TGAGGATTTA TATTGTTCGG 
GGCATAGATC TCCAGCCTCA GGATAACAAT GGCCTGTGTG ACCCTTATAT AAAAATATCC 
CTGAGTAAAA AAGTCATTGA AGATCGAGAT AATTACATTC CAAATACACT CAACCCAATT 
TTTGGAAGGA TGTATGAGCT AAGCTGCTTC TTACCTCAAG AAAAAGACCT GAAAATTTCT 
GTCTATGATT ATGACACACT TACTCGTGAT GAAAAAGTAG GAGAAACTAT CATTGATCTA 
GAAAATCGCT TCCTTTCCCG ATTTGGGTCG AATTGTGGCA TAGCAGAGCA ATATTGCATT 
TCTGGAGTCA ATACTTGGAG AGATCAACTA AAACCAACCC AATTGTTATG CAATGTAGCT 
AAGTTTAAAG GATTGCCACT CCCTATTCTT TCTGAAGATG GAAATAGGCT AAGATTTGGA 
GGTCGAGAGT ACACCTTGGA AGAATTTGAG GCCAACAGAA ATCTGCACCA GCACCTGGGA 
CCTGCAGAGG AGCGTCTTGC TCTGTATATC CTTCGGACTC AGGGTCAAGT CCCTGAGCAT 
GTGGAAACAA GGACTTTGTA CAGCACCTTC CAACCAAATA TTTCACAGGG AAAACTTCAG 
ATGTGGGTGG ATGTTTTCCC CAAAAGTTTG GGGCCACCAG GCCCTCCTTT CAATATCACT 
CCCCGGAAGG CCAAGAGGTA TGTCCTACGT GTGATCATTT GGAACACTAA AGATGTCATT 
CTTGATGAGA AAAGTATCAC AGGAGAAGAG ATGACTGACA TCTATGTGAA AGGCTGGATT 
CCTGGGAATG AAGAAAACAA GCAGAAGACA GATGTCCATT ACAGGTCATT GGATGGTGAA 
GGAAACTTTA ATTGGAGATT TGTTTTCCCT TTTGAATACC TGCCTGCTGA ACAACTCTGT 
ATTGTTTCTA AAAAAGAACA TTTCTGGAGT ATTGACCAGA CTGAATTTCG AGTTCCTCCC 
AGACTCATCA TTCAGATATG GGACAATGAT AAGTTTTCTT TAGATGACTA CTTGGGTTTT 
CTGGAACTTG ATTTACACAA CACAATTATC CCTGCAAAGT CTTCAGAAAA GTGCAAATTG 
GACATGATTT CAGAGTATAG GGCAGTGAAT CCTCTAAAGT CCAAGACAGC CTCACTTTTT 
GAACAGAAAT CGATGAAAGG ATGGTGGCCA TGCTATGCAG AAAAAGATGG CGCACGTGTG 
ATGACTGGTA AAGTGGAGAT GACTTTGGAA GTCCTCAATG AGAAGGAGGT TGATGAAAGA 
CCAGCTGGGA AAGGAAGGGA TGAGCCCAAC ATGAACCCCA AGCTAGAATT ACCAAATCGA 
CCAGAAACTT CATTCCTTTG GTTTACCAAC CCATGCAAGA CAATGAAATT CATCGTGTGG 
CGTCGATTTA AATGGGTCAT CATTGGCTTA CTCATCCTGC TGGTCTTGTT GCTGTTTTTG 
GCAGTACTCC TATATTCTCT GCCGAACTAT ATATCAATGA AGATCGTGAG ACCAAATGCA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_007478796.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007478858.1 Monodelphis domestica myoferlin (MYOF), transcript variant X8, mRNA.

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CloneID	OMc00888
Clone ID Related Accession (Same CDS sequence)	XM_007478796.2
Accession Version	XM_007478796.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	myoferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007478796.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGGG TCATTGTGGA GTCTGCGAGT GATATCCCTA AAGGGAAATT TGGAAAACCG 
GATCCAATTG TCTCAGTTAT TTTTAAGGAT GAGAAGAAGA AAACGAAGAA AATTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATATTT GAATTTGATT TGAAGGGCAT ACCATTGGAC 
TTTTCATCTA CTCTTGGCAT CACTGTGAAA GATTTCGAGA CAATTGGACA AAATAAACTG 
ATTGGCGTTG CAACAATATC ACTGAAGGAC TTGATAGGTG GCCAGAGTCG ATCTCTTCCT 
TATAAACTCA TATCTTTGGT AAATGAAAAG GGTCAAGATA CTGGGGCTAC AATCGATCTG 
GTGGTTGCCT ATGATCCACC AGCAGCCATG GCAACAAATC CAAATGAACT AAGTGGGATC 
ACCATAGAAG GCACGGGAGA AGAAGAGGAA GAAGACACAG GAGATGAAGA TACTGCATTC 
AAAGTCATTG CCCCAAGGGT TCCAGCAACA AATATTTCTG AAGCCCAGCT GGCCAGGAGG 
ATTACAAAAG GAAAGAATAC CCGAAAGATC CTTTCCAATA AGCCACAAGA CTTCCAGATT 
CGTGTTAGAG TGATTGAAGG GCGCCAATTA AGTGGGAATA ACATCAAACC AGTGGTCAAA 
GTCCATGTCT GTGGTCAGAC ACATCGAACA AGAATAAAAA GAGGGAATAA TCCATTTTTT 
GACGAATTAT TTTTCTACAA TGTCCACATG ACCCCTCTTG AACTGGTTGA TGAGACAATA 
AGCATTCGGG TATATGATTC CCATTCTTTG AGAGCAGACT GTCTGATGGG AGAATTTAAG 
ATTGATATTG GTTACATTTA TGATGAACCT GGTCATGCTG TCATGAGAAA ATGGCTTCTT 
CTAAATGACC CTGAAGATAT AAGTTCTGGT GCTAAAGGTT ACATGAAAGT CAGCATGTTT 
GTCCTTGGAA CAGGAGATGA ACCACCAACA GAGAAGAGAG ATCGAGATAA TGACAGTGAT 
GATGTGGAGA GTAATTTGTT ACTTCCTGCT GGAATTGCCA TGCGGTGGGT CACTTTCCTC 
CTGAAAATTT ACAGAGCTGA GGATATTCCT CAGATGGATG ATGCCTTTTC ACAGACTGTC 
AAAGAAATAT TTGGAGCCAA TGTAGACAAG AAAAACTTAG TGGATCCATT TGTAGAAGTT 
TGTTTTGCTG GGAAAAAGGT TTCCACAGGC ATAATAGAGA AAAATGCAAA TCCAGAGTGG 
AATCAAGTTG TCAATCTTCA AATCAAGTTT CCTTCAATGT GTGAAAAAAT AAAGTTAACA 
GTATATGACT GGGACCGCCT TACCAAGAAT GATGTAGTAG GGACAACCTA TCTGTATCTT 
TCTAAAATTG CTGCATCGGG TGGGGAAGTT GAAGAGAAAA CAGGAGAAAC AGAAGTGGGC 
TTTGTACCAA CATTTGGACC TTGTTATCTA AATCTTTATG GAAGTCCAAG AGAATACACT 
GGATTCCCAG ATCCATATGA TGACTTGAAT TCTGGAAAGG GTGAAGGAGT TGCCTACAGA 
GGTCGAATCT TGGTAGAACT GAATACTTTA CTTGAGAAAA CACCCCCTGA CAAGAAGGTT 
GAAACCATAT CCAATGATGA TTTGCTGGTT GTTGAGAAAT ATCAGCGTAG GCGAAAATAT 
AGCCTGTCTG CGGTATTTCA TTCTGCAACC ATGCTGCAAG ATATTGGAGA GGCCATTCAG 
TTTGAAGTCA GCATTGGAAA TTACGGCAAT AAGTTTGATG CTACCTGTAA GCCTTTGGCA 
TCGACAACTC AATACAGTCG GGCTGTGTTT GATGGAAATT TCTATTATTA TTTGCCATGG 
GCCCAGAAGA AGCCAGTGGT GACTCTAACT TCTTATTGGG AAGACATTAG TCACAGACTG 
GATGCAGTGA ACACCATTCT TGTTATGGCA GAACGGCTGC ACGTGAACAT TGAGAATTTA 
AAATCAGGAA TACAAGCCAA AATGTCTGGG AATCATTTGG CTGAAATGTG GCTGAAGTTG 
ATTGATGAAC TTATCGAAGA TACAAGCATG CCATTGCCTT CACTAGAAGG GAAATCTAAT 
GTCACCATTC TAGACACTCA GATCCAAAAG CTGCGTCTTC GATCCCTTAT CCAGATTCAA 
GAAGCAGCTA GAAAAATGAG GTCTGAAGCC AAACATGTGA AGTCTACTTT GACAGAAATT 
GAAGATTGGT TTGATAAGCT AATGCAACTT TCTGAAGAGC CACAAAACAG TATGCCCGAT 
ATTATCATAT GGATGATCCG AGGAGAGAAA AGATTGGCCT ATGCTAGAAT TCCTGCACAC 
CAGGTTTTAT ATTCTACTAC CAGTGAAGAA GCTTCTGGAA AGTACTGTGG GAAGACCCAG 
ACCATCTTTT TGAAGTACCC ACAGGACAGG AGCTATGGTG TGAAGGTTCC AGCAGAATTG 
CGGGTTAACA TTTGGCTTGG ATTAAGTGCT GTTGAAAAGA AGTTTAACAG CTTTGCTGAA 
GGAACATTCA CTGTCTTTGC AGAAATGTAT GAAAATCAAG CTTTGATGTT TGGAAAATGG 
GGCACTTCTG GACTTGTGGG TCGACATAAA TTTTCAGATG TCACAGGCAA GATAAAGCTC 
AAGAAAGAGT TCTTTTTGCC CCCTAAAGGC TGGGAATGGG AAGGTGAATG GATGGTGGAT 
CCTGAAAGAA GTTTATTGAC TGAAGCGGAT GCTGGCCACA CACACTTTAC AGATGAAGTG 
TATCAGAATG AAAGCCGCTA TCCAGGTGGA GAATGGAAGG CAATGGAGGA CAGCTACACA 
GATGTGAATG GAGAAAAAGC AGCATCTCCT AATGAGATGA CTTGCCCCCC TGGCTGGGAA 
TGGGAAGATG ATGCTTGGAT GTATGATATA AACCGAGCTG TAGATGAAAA AGGCTGGGAA 
TATGGAATTA CTATTCCTCC TGATAACAAG CCAAAGTCCT GGGTTGCTGC AGAGAAGATG 
TATCATACAC ACAGGCGGCG GCGAATGGTC CGCAAACGCA AAAAGGATGT GACACAGTCG 
GCTGCAAGTA CTGCAAGGGG GTGGGAAGCA CTAGAAGATA AAGAAGGCTG GGAATATGCA 
TCTTTAATTG GATGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACTTT TCGTCGAAGA 
CGCTGGAGAA GAAAGATGGC TCCTTCTGAA ACACATGGTT CAGCTGCTAT CTTTAAACTT 
GAAGGTGCCA TTGGAGCAGA CACTACTGAA GATGGAGATG AAAAGAGCAC GGAGAAAAAC 
AAACATAGTG CAACAACCGT GTTTGGTGCA AACACTCCAA TTGTTTCCTG TAATTTTGAC 
AGAGTATATA ATTATCATCT TCGCTGCTAT ATATATCAAG CTCGAAATCT TATGGCTTTA 
GATAAAGATA GTTTTTCAGA TCCCTATGCT CATGTTTCAT TCCTCCATCG GAGCCAAACA 
ACTGAAATCA TCCACTCAAC ACTGAATCCA ACCTGGGACC AAACTCTAAT ATTTGATGAC 
ATTGAAATTT TTGGAGAACC ACAAACAATC ATACAAAACC CACCCAAAGT TGTCATTGAA 
CTCTTTGACA ATGACCAAGT GGGCAAGGAT GAATTTTTAG GTCGAAGTTT TTGCTCCCCA 
GTTGTCAAGC TAAATCCAGA AGTTGACATC ACCCCTAAGC TTCTCTGGCA ACCAGTCATA 
AATGGAGATA AGGCTTGTGG GGATGTACTT GTAGCTGCTG AGCTTATTCT CAAAGATAAG 
GTTGGCTCCA ATCTTCCAAT TCTCCCATCT CAGAGGGCTC CAAATCTCTA CATGGTTCCT 
CAAGGAATTA GGCCTGTGGT TCAACTCACT GCTATTGAGA TTTTAGCTTG GGGTTTAAGA 
AACATGAAAA ACTACCAGAT GGCTTCTGTG ACTTCCCCAA GTCTTATCAT AGAGTGTGGA 
GGAGAAAGGA TAGAATCAGC ATTGATCAAA AACCTCAAGA AGACACCCAA CTTTCCTAGT 
TCTGTTCTCT TCATGAAAGT GCTATTGCCC AAGGAAGAAT TGTACACACC ACCATTGGTT 
ATCAAAGTGA TCGACCACAG ACAATTTGGG CGAAAGCCCA TCGTGGGTCA GTGCACCATC 
GACCTTCTTG AACAGTTTCG TTGTGACCCT TATGCATTTA AGGAAGATAT TGCACCCCAG 
GTAAAAGCTT CTATCTTGTC TGCCCCACTT AGCCGGGATG TGGTTATTGA AGTTGAGGAC 
ACAAAACCAT TGCTTCCAAC CAAGTTAACT GAAAAGGAGG AAGAAGTTGT TGACTGGTGG 
AGCAAATTTT ATGCATCTGT TGGAGAACCT GAAAAGTGTG GGCAATATAT CCAGAAGGAA 
TATGCCAAAC TAAAGGTATA TAATTGTGAA CTAGAGAGTG TTCCAGAGTT TAAGGGTCTG 
ACAGACTTTT CAGATACCTT TAAGCTTTAC AGAGGGAAGT CTGATGAGAT TGATGATCCT 
TCAGTGGTAG GAGAGTTCAA GGGCTCCTTT AGAATCTATC CACTGCCTGA TGATCCAAAT 
GTGCCAGCTC CTCCCAGGCA GTTTCGGGAA TTACCTGACA GTGTCCCACA GGAATGCATT 
GTGAGGATTT ATATTGTTCG GGGCATAGAT CTCCAGCCTC AGGATAACAA TGGCCTGTGT 
GACCCTTATA TAAAAATATC CCTGAGTAAA AAAGTCATTG AAGATCGAGA TAATTACATT 
CCAAATACAC TCAACCCAAT TTTTGGAAGG ATGTATGAGC TAAGCTGCTT CTTACCTCAA 
GAAAAAGACC TGAAAATTTC TGTCTATGAT TATGACACAC TTACTCGTGA TGAAAAAGTA 
GGAGAAACTA TCATTGATCT AGAAAATCGC TTCCTTTCCC GATTTGGGTC GAATTGTGGC 
ATAGCAGAGC AATATTGCAT TTCTGGAGTC AATACTTGGA GAGATCAACT AAAACCAACC 
CAATTGTTAT GCAATGTAGC TAAGTTTAAA GGATTGCCAC TCCCTATTCT TTCTGAAGAT 
GGAAATAGGC TAAGATTTGG AGGTCGAGAG TACACCTTGG AAGAATTTGA GGCCAACAGA 
AATCTGCACC AGCACCTGGG ACCTGCAGAG GAGCGTCTTG CTCTGTATAT CCTTCGGACT 
CAGGGTCAAG TCCCTGAGCA TGTGGAAACA AGGACTTTGT ACAGCACCTT CCAACCAAAT 
ATTTCACAGG GAAAACTTCA GATGTGGGTG GATGTTTTCC CCAAAAGTTT GGGGCCACCA 
GGCCCTCCTT TCAATATCAC TCCCCGGAAG GCCAAGAGGT ATGTCCTACG TGTGATCATT 
TGGAACACTA AAGATGTCAT TCTTGATGAG AAAAGTATCA CAGGAGAAGA GATGACTGAC 
ATCTATGTGA AAGGCTGGAT TCCTGGGAAT GAAGAAAACA AGCAGAAGAC AGATGTCCAT 
TACAGGTCAT TGGATGGTGA AGGAAACTTT AATTGGAGAT TTGTTTTCCC TTTTGAATAC 
CTGCCTGCTG AACAACTCTG TATTGTTTCT AAAAAAGAAC ATTTCTGGAG TATTGACCAG 
ACTGAATTTC GAGTTCCTCC CAGACTCATC ATTCAGATAT GGGACAATGA TAAGTTTTCT 
TTAGATGACT ACTTGGGTTT TCTGGAACTT GATTTACACA ACACAATTAT CCCTGCAAAG 
TCTTCAGAAA AGTGCAAATT GGACATGATT TCAGAGTATA GGGCAGTGAA TCCTCTAAAG 
TCCAAGACAG CCTCACTTTT TGAACAGAAA TCGATGAAAG GATGGTGGCC ATGCTATGCA 
GAAAAAGATG GCGCACGTGT GATGACTGGT AAAGTGGAGA TGACTTTGGA AGTCCTCAAT 
GAGAAGGAGG TTGATGAAAG ACCAGCTGGG AAAGGAAGGG ATGAGCCCAA CATGAACCCC 
AAGCTAGAAT TACCAAATCG ACCAGAAACT TCATTCCTTT GGTTTACCAA CCCATGCAAG 
ACAATGAAAT TCATCGTGTG GCGTCGATTT AAATGGGTCA TCATTGGCTT ACTCATCCTG 
CTGGTCTTGT TGCTGTTTTT GGCAGTACTC CTATATTCTC TGCCGAACTA TATATCAATG 
AAGATCGTGA GACCAAATGC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007478858.1
CDS	179..6325
Translation	MLRVIVESASDIPKGKFGKPDPIVSVIFKDEKKKTKKIDNELNPVWNEIFEFDLKGIPLDFSSTLGITVKDFETIGQNKLIGVATISLKDLIGGQSRSLPYKLISLVNEKGQDTGATIDLVVAYDPPAAMATNPNELSGITIEGTGEEEEEDTGDEDTAFKVIAPRVPATNISEAQLARRITKGKNTRKILSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHMTPLELVDETISIRVYDSHSLRADCLMGEFKIDIGYIYDEPGHAVMRKWLLLNDPEDISSGAKGYMKVSMFVLGTGDEPPTEKRDRDNDSDDVESNLLLPAGIAMRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGANVDKKNLVDPFVEVCFAGKKVSTGIIEKNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEEKTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDDLNSGKGEGVAYRGRILVELNTLLEKTPPDKKVETISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNFYYYLPWAQKKPVVTLTSYWEDISHRLDAVNTILVMAERLHVNIENLKSGIQAKMSGNHLAEMWLKLIDELIEDTSMPLPSLEGKSNVTILDTQIQKLRLRSLIQIQEAARKMRSEAKHVKSTLTEIEDWFDKLMQLSEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTTSEEASGKYCGKTQTIFLKYPQDRSYGVKVPAELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKKEFFLPPKGWEWEGEWMVDPERSLLTEADAGHTHFTDEVYQNESRYPGGEWKAMEDSYTDVNGEKAASPNEMTCPPGWEWEDDAWMYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRMVRKRKKDVTQSAASTARGWEALEDKEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGSAAIFKLEGAIGADTTEDGDEKSTEKNKHSATTVFGANTPIVSCNFDRVYNYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSQTTEIIHSTLNPTWDQTLIFDDIEIFGEPQTIIQNPPKVVIELFDNDQVGKDEFLGRSFCSPVVKLNPEVDITPKLLWQPVINGDKACGDVLVAAELILKDKVGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIIECGGERIESALIKNLKKTPNFPSSVLFMKVLLPKEELYTPPLVIKVIDHRQFGRKPIVGQCTIDLLEQFRCDPYAFKEDIAPQVKASILSAPLSRDVVIEVEDTKPLLPTKLTEKEEEVVDWWSKFYASVGEPEKCGQYIQKEYAKLKVYNCELESVPEFKGLTDFSDTFKLYRGKSDEIDDPSVVGEFKGSFRIYPLPDDPNVPAPPRQFRELPDSVPQECIVRIYIVRGIDLQPQDNNGLCDPYIKISLSKKVIEDRDNYIPNTLNPIFGRMYELSCFLPQEKDLKISVYDYDTLTRDEKVGETIIDLENRFLSRFGSNCGIAEQYCISGVNTWRDQLKPTQLLCNVAKFKGLPLPILSEDGNRLRFGGREYTLEEFEANRNLHQHLGPAEERLALYILRTQGQVPEHVETRTLYSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKRYVLRVIIWNTKDVILDEKSITGEEMTDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFEYLPAEQLCIVSKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHNTIIPAKSSEKCKLDMISEYRAVNPLKSKTASLFEQKSMKGWWPCYAEKDGARVMTGKVEMTLEVLNEKEVDERPAGKGRDEPNMNPKLELPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLILLVLLLFLAVLLYSLPNYISMKIVRPNAK

Target ORF information:

RefSeq Version	XM_007478796.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica myoferlin (MYOF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007478796.2

ORF Insert Sequence:

ATGCTTCGGG TCATTGTGGA GTCTGCGAGT GATATCCCTA AAGGGAAATT TGGAAAACCG 
GATCCAATTG TCTCAGTTAT TTTTAAGGAT GAGAAGAAGA AAACGAAGAA AATTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATATTT GAATTTGATT TGAAGGGCAT ACCATTGGAC 
TTTTCATCTA CTCTTGGCAT CACTGTGAAA GATTTCGAGA CAATTGGACA AAATAAACTG 
ATTGGCGTTG CAACAATATC ACTGAAGGAC TTGATAGGTG GCCAGAGTCG ATCTCTTCCT 
TATAAACTCA TATCTTTGGT AAATGAAAAG GGTCAAGATA CTGGGGCTAC AATCGATCTG 
GTGGTTGCCT ATGATCCACC AGCAGCCATG GCAACAAATC CAAATGAACT AAGTGGGATC 
ACCATAGAAG GCACGGGAGA AGAAGAGGAA GAAGACACAG GAGATGAAGA TACTGCATTC 
AAAGTCATTG CCCCAAGGGT TCCAGCAACA AATATTTCTG AAGCCCAGCT GGCCAGGAGG 
ATTACAAAAG GAAAGAATAC CCGAAAGATC CTTTCCAATA AGCCACAAGA CTTCCAGATT 
CGTGTTAGAG TGATTGAAGG GCGCCAATTA AGTGGGAATA ACATCAAACC AGTGGTCAAA 
GTCCATGTCT GTGGTCAGAC ACATCGAACA AGAATAAAAA GAGGGAATAA TCCATTTTTT 
GACGAATTAT TTTTCTACAA TGTCCACATG ACCCCTCTTG AACTGGTTGA TGAGACAATA 
AGCATTCGGG TATATGATTC CCATTCTTTG AGAGCAGACT GTCTGATGGG AGAATTTAAG 
ATTGATATTG GTTACATTTA TGATGAACCT GGTCATGCTG TCATGAGAAA ATGGCTTCTT 
CTAAATGACC CTGAAGATAT AAGTTCTGGT GCTAAAGGTT ACATGAAAGT CAGCATGTTT 
GTCCTTGGAA CAGGAGATGA ACCACCAACA GAGAAGAGAG ATCGAGATAA TGACAGTGAT 
GATGTGGAGA GTAATTTGTT ACTTCCTGCT GGAATTGCCA TGCGGTGGGT CACTTTCCTC 
CTGAAAATTT ACAGAGCTGA GGATATTCCT CAGATGGATG ATGCCTTTTC ACAGACTGTC 
AAAGAAATAT TTGGAGCCAA TGTAGACAAG AAAAACTTAG TGGATCCATT TGTAGAAGTT 
TGTTTTGCTG GGAAAAAGGT TTCCACAGGC ATAATAGAGA AAAATGCAAA TCCAGAGTGG 
AATCAAGTTG TCAATCTTCA AATCAAGTTT CCTTCAATGT GTGAAAAAAT AAAGTTAACA 
GTATATGACT GGGACCGCCT TACCAAGAAT GATGTAGTAG GGACAACCTA TCTGTATCTT 
TCTAAAATTG CTGCATCGGG TGGGGAAGTT GAAGAGAAAA CAGGAGAAAC AGAAGTGGGC 
TTTGTACCAA CATTTGGACC TTGTTATCTA AATCTTTATG GAAGTCCAAG AGAATACACT 
GGATTCCCAG ATCCATATGA TGACTTGAAT TCTGGAAAGG GTGAAGGAGT TGCCTACAGA 
GGTCGAATCT TGGTAGAACT GAATACTTTA CTTGAGAAAA CACCCCCTGA CAAGAAGGTT 
GAAACCATAT CCAATGATGA TTTGCTGGTT GTTGAGAAAT ATCAGCGTAG GCGAAAATAT 
AGCCTGTCTG CGGTATTTCA TTCTGCAACC ATGCTGCAAG ATATTGGAGA GGCCATTCAG 
TTTGAAGTCA GCATTGGAAA TTACGGCAAT AAGTTTGATG CTACCTGTAA GCCTTTGGCA 
TCGACAACTC AATACAGTCG GGCTGTGTTT GATGGAAATT TCTATTATTA TTTGCCATGG 
GCCCAGAAGA AGCCAGTGGT GACTCTAACT TCTTATTGGG AAGACATTAG TCACAGACTG 
GATGCAGTGA ACACCATTCT TGTTATGGCA GAACGGCTGC ACGTGAACAT TGAGAATTTA 
AAATCAGGAA TACAAGCCAA AATGTCTGGG AATCATTTGG CTGAAATGTG GCTGAAGTTG 
ATTGATGAAC TTATCGAAGA TACAAGCATG CCATTGCCTT CACTAGAAGG GAAATCTAAT 
GTCACCATTC TAGACACTCA GATCCAAAAG CTGCGTCTTC GATCCCTTAT CCAGATTCAA 
GAAGCAGCTA GAAAAATGAG GTCTGAAGCC AAACATGTGA AGTCTACTTT GACAGAAATT 
GAAGATTGGT TTGATAAGCT AATGCAACTT TCTGAAGAGC CACAAAACAG TATGCCCGAT 
ATTATCATAT GGATGATCCG AGGAGAGAAA AGATTGGCCT ATGCTAGAAT TCCTGCACAC 
CAGGTTTTAT ATTCTACTAC CAGTGAAGAA GCTTCTGGAA AGTACTGTGG GAAGACCCAG 
ACCATCTTTT TGAAGTACCC ACAGGACAGG AGCTATGGTG TGAAGGTTCC AGCAGAATTG 
CGGGTTAACA TTTGGCTTGG ATTAAGTGCT GTTGAAAAGA AGTTTAACAG CTTTGCTGAA 
GGAACATTCA CTGTCTTTGC AGAAATGTAT GAAAATCAAG CTTTGATGTT TGGAAAATGG 
GGCACTTCTG GACTTGTGGG TCGACATAAA TTTTCAGATG TCACAGGCAA GATAAAGCTC 
AAGAAAGAGT TCTTTTTGCC CCCTAAAGGC TGGGAATGGG AAGGTGAATG GATGGTGGAT 
CCTGAAAGAA GTTTATTGAC TGAAGCGGAT GCTGGCCACA CACACTTTAC AGATGAAGTG 
TATCAGAATG AAAGCCGCTA TCCAGGTGGA GAATGGAAGG CAATGGAGGA CAGCTACACA 
GATGTGAATG GAGAAAAAGC AGCATCTCCT AATGAGATGA CTTGCCCCCC TGGCTGGGAA 
TGGGAAGATG ATGCTTGGAT GTATGATATA AACCGAGCTG TAGATGAAAA AGGCTGGGAA 
TATGGAATTA CTATTCCTCC TGATAACAAG CCAAAGTCCT GGGTTGCTGC AGAGAAGATG 
TATCATACAC ACAGGCGGCG GCGAATGGTC CGCAAACGCA AAAAGGATGT GACACAGTCG 
GCTGCAAGTA CTGCAAGGGG GTGGGAAGCA CTAGAAGATA AAGAAGGCTG GGAATATGCA 
TCTTTAATTG GATGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACTTT TCGTCGAAGA 
CGCTGGAGAA GAAAGATGGC TCCTTCTGAA ACACATGGTT CAGCTGCTAT CTTTAAACTT 
GAAGGTGCCA TTGGAGCAGA CACTACTGAA GATGGAGATG AAAAGAGCAC GGAGAAAAAC 
AAACATAGTG CAACAACCGT GTTTGGTGCA AACACTCCAA TTGTTTCCTG TAATTTTGAC 
AGAGTATATA ATTATCATCT TCGCTGCTAT ATATATCAAG CTCGAAATCT TATGGCTTTA 
GATAAAGATA GTTTTTCAGA TCCCTATGCT CATGTTTCAT TCCTCCATCG GAGCCAAACA 
ACTGAAATCA TCCACTCAAC ACTGAATCCA ACCTGGGACC AAACTCTAAT ATTTGATGAC 
ATTGAAATTT TTGGAGAACC ACAAACAATC ATACAAAACC CACCCAAAGT TGTCATTGAA 
CTCTTTGACA ATGACCAAGT GGGCAAGGAT GAATTTTTAG GTCGAAGTTT TTGCTCCCCA 
GTTGTCAAGC TAAATCCAGA AGTTGACATC ACCCCTAAGC TTCTCTGGCA ACCAGTCATA 
AATGGAGATA AGGCTTGTGG GGATGTACTT GTAGCTGCTG AGCTTATTCT CAAAGATAAG 
GTTGGCTCCA ATCTTCCAAT TCTCCCATCT CAGAGGGCTC CAAATCTCTA CATGGTTCCT 
CAAGGAATTA GGCCTGTGGT TCAACTCACT GCTATTGAGA TTTTAGCTTG GGGTTTAAGA 
AACATGAAAA ACTACCAGAT GGCTTCTGTG ACTTCCCCAA GTCTTATCAT AGAGTGTGGA 
GGAGAAAGGA TAGAATCAGC ATTGATCAAA AACCTCAAGA AGACACCCAA CTTTCCTAGT 
TCTGTTCTCT TCATGAAAGT GCTATTGCCC AAGGAAGAAT TGTACACACC ACCATTGGTT 
ATCAAAGTGA TCGACCACAG ACAATTTGGG CGAAAGCCCA TCGTGGGTCA GTGCACCATC 
GACCTTCTTG AACAGTTTCG TTGTGACCCT TATGCATTTA AGGAAGATAT TGCACCCCAG 
GTAAAAGCTT CTATCTTGTC TGCCCCACTT AGCCGGGATG TGGTTATTGA AGTTGAGGAC 
ACAAAACCAT TGCTTCCAAC CAAGTTAACT GAAAAGGAGG AAGAAGTTGT TGACTGGTGG 
AGCAAATTTT ATGCATCTGT TGGAGAACCT GAAAAGTGTG GGCAATATAT CCAGAAGGAA 
TATGCCAAAC TAAAGGTATA TAATTGTGAA CTAGAGAGTG TTCCAGAGTT TAAGGGTCTG 
ACAGACTTTT CAGATACCTT TAAGCTTTAC AGAGGGAAGT CTGATGAGAT TGATGATCCT 
TCAGTGGTAG GAGAGTTCAA GGGCTCCTTT AGAATCTATC CACTGCCTGA TGATCCAAAT 
GTGCCAGCTC CTCCCAGGCA GTTTCGGGAA TTACCTGACA GTGTCCCACA GGAATGCATT 
GTGAGGATTT ATATTGTTCG GGGCATAGAT CTCCAGCCTC AGGATAACAA TGGCCTGTGT 
GACCCTTATA TAAAAATATC CCTGAGTAAA AAAGTCATTG AAGATCGAGA TAATTACATT 
CCAAATACAC TCAACCCAAT TTTTGGAAGG ATGTATGAGC TAAGCTGCTT CTTACCTCAA 
GAAAAAGACC TGAAAATTTC TGTCTATGAT TATGACACAC TTACTCGTGA TGAAAAAGTA 
GGAGAAACTA TCATTGATCT AGAAAATCGC TTCCTTTCCC GATTTGGGTC GAATTGTGGC 
ATAGCAGAGC AATATTGCAT TTCTGGAGTC AATACTTGGA GAGATCAACT AAAACCAACC 
CAATTGTTAT GCAATGTAGC TAAGTTTAAA GGATTGCCAC TCCCTATTCT TTCTGAAGAT 
GGAAATAGGC TAAGATTTGG AGGTCGAGAG TACACCTTGG AAGAATTTGA GGCCAACAGA 
AATCTGCACC AGCACCTGGG ACCTGCAGAG GAGCGTCTTG CTCTGTATAT CCTTCGGACT 
CAGGGTCAAG TCCCTGAGCA TGTGGAAACA AGGACTTTGT ACAGCACCTT CCAACCAAAT 
ATTTCACAGG GAAAACTTCA GATGTGGGTG GATGTTTTCC CCAAAAGTTT GGGGCCACCA 
GGCCCTCCTT TCAATATCAC TCCCCGGAAG GCCAAGAGGT ATGTCCTACG TGTGATCATT 
TGGAACACTA AAGATGTCAT TCTTGATGAG AAAAGTATCA CAGGAGAAGA GATGACTGAC 
ATCTATGTGA AAGGCTGGAT TCCTGGGAAT GAAGAAAACA AGCAGAAGAC AGATGTCCAT 
TACAGGTCAT TGGATGGTGA AGGAAACTTT AATTGGAGAT TTGTTTTCCC TTTTGAATAC 
CTGCCTGCTG AACAACTCTG TATTGTTTCT AAAAAAGAAC ATTTCTGGAG TATTGACCAG 
ACTGAATTTC GAGTTCCTCC CAGACTCATC ATTCAGATAT GGGACAATGA TAAGTTTTCT 
TTAGATGACT ACTTGGGTTT TCTGGAACTT GATTTACACA ACACAATTAT CCCTGCAAAG 
TCTTCAGAAA AGTGCAAATT GGACATGATT TCAGAGTATA GGGCAGTGAA TCCTCTAAAG 
TCCAAGACAG CCTCACTTTT TGAACAGAAA TCGATGAAAG GATGGTGGCC ATGCTATGCA 
GAAAAAGATG GCGCACGTGT GATGACTGGT AAAGTGGAGA TGACTTTGGA AGTCCTCAAT 
GAGAAGGAGG TTGATGAAAG ACCAGCTGGG AAAGGAAGGG ATGAGCCCAA CATGAACCCC 
AAGCTAGAAT TACCAAATCG ACCAGAAACT TCATTCCTTT GGTTTACCAA CCCATGCAAG 
ACAATGAAAT TCATCGTGTG GCGTCGATTT AAATGGGTCA TCATTGGCTT ACTCATCCTG 
CTGGTCTTGT TGCTGTTTTT GGCAGTACTC CTATATTCTC TGCCGAACTA TATATCAATG 
AAGATCGTGA GACCAAATGC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_016426154.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016281640.1 Monodelphis domestica myoferlin (MYOF), transcript variant X1, mRNA.

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CloneID	OMc38444
Clone ID Related Accession (Same CDS sequence)	XM_016426154.1
Accession Version	XM_016426154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	myoferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTTG GGGTGTTCAT TTTGATGATC TTGAAGGTCT CTTCCAGCTA TAAATACTAT 
CATCCTATTA TCTTTCAGCT GGGGGTATGC AGAGGGATTC ATGAAAGAGG GCCTAAAAGA 
AAGGAAGGAG CTTCAAAAGA TGAAGATGAG AAGAAGAAAA CGAAGAAAAT TGATAATGAA 
CTCAACCCTG TCTGGAATGA GATATTTGAA TTTGATTTGA AGGGCATACC ATTGGACTTT 
TCATCTACTC TTGGCATCAC TGTGAAAGAT TTCGAGACAA TTGGACAAAA TAAACTGATT 
GGCGTTGCAA CAATATCACT GAAGGACTTG ATAGGTGGCC AGAGTCGATC TCTTCCTTAT 
AAACTCATAT CTTTGGTAAA TGAAAAGGGT CAAGATACTG GGGCTACAAT CGATCTGGTG 
GTTGCCTATG ATCCACCAGC AGCCATGGCA ACAAATCCAA ATGAACTAAG TGGGATCACC 
ATAGAAGGCA CGGGAGAAGA AGAGGAAGAA GACACAGGAG ATGAAGATAC TGCATTCAAA 
GTCATTGCCC CAAGGGTTCC AGCAACAAAT ATTTCTGAAG CCCAGCTGGC CAGGAGGATT 
ACAAAAGGAA AGAATACCCG AAAGATCCTT TCCAATAAGC CACAAGACTT CCAGATTCGT 
GTTAGAGTGA TTGAAGGGCG CCAATTAAGT GGGAATAACA TCAAACCAGT GGTCAAAGTC 
CATGTCTGTG GTCAGACACA TCGAACAAGA ATAAAAAGAG GGAATAATCC ATTTTTTGAC 
GAATTATTTT TCTACAATGT CCACATGACC CCTCTTGAAC TGGTTGATGA GACAATAAGC 
ATTCGGGTAT ATGATTCCCA TTCTTTGAGA GCAGACTGTC TGATGGGAGA ATTTAAGATT 
GATATTGGTT ACATTTATGA TGAACCTGGT CATGCTGTCA TGAGAAAATG GCTTCTTCTA 
AATGACCCTG AAGATATAAG TTCTGGTGCT AAAGGTTACA TGAAAGTCAG CATGTTTGTC 
CTTGGAACAG GAGATGAACC ACCAACAGAG AAGAGAGATC GAGATAATGA CAGTGATGAT 
GTGGAGAGTA ATTTGTTACT TCCTGCTGGA ATTGCCATGC GGTGGGTCAC TTTCCTCCTG 
AAAATTTACA GAGCTGAGGA TATTCCTCAG ATGGATGATG CCTTTTCACA GACTGTCAAA 
GAAATATTTG GAGCCAATGT AGACAAGAAA AACTTAGTGG ATCCATTTGT AGAAGTTTGT 
TTTGCTGGGA AAAAGGTTTC CACAGGCATA ATAGAGAAAA ATGCAAATCC AGAGTGGAAT 
CAAGTTGTCA ATCTTCAAAT CAAGTTTCCT TCAATGTGTG AAAAAATAAA GTTAACAGTA 
TATGACTGGG ACCGCCTTAC CAAGAATGAT GTAGTAGGGA CAACCTATCT GTATCTTTCT 
AAAATTGCTG CATCGGGTGG GGAAGTTGAA GATTCCTCAC CTTCGGGAGC TTGGGCTGCA 
TCATCCACAG AGAAAACAGG AGAAACAGAA GTGGGCTTTG TACCAACATT TGGACCTTGT 
TATCTAAATC TTTATGGAAG TCCAAGAGAA TACACTGGAT TCCCAGATCC ATATGATGAC 
TTGAATTCTG GAAAGGGTGA AGGAGTTGCC TACAGAGGTC GAATCTTGGT AGAACTGAAT 
ACTTTACTTG AGAAAACACC CCCTGACAAG AAGGTTGAAA CCATATCCAA TGATGATTTG 
CTGGTTGTTG AGAAATATCA GCGTAGGCGA AAATATAGCC TGTCTGCGGT ATTTCATTCT 
GCAACCATGC TGCAAGATAT TGGAGAGGCC ATTCAGTTTG AAGTCAGCAT TGGAAATTAC 
GGCAATAAGT TTGATGCTAC CTGTAAGCCT TTGGCATCGA CAACTCAATA CAGTCGGGCT 
GTGTTTGATG GAAATTTCTA TTATTATTTG CCATGGGCCC AGAAGAAGCC AGTGGTGACT 
CTAACTTCTT ATTGGGAAGA CATTAGTCAC AGACTGGATG CAGTGAACAC CATTCTTGTT 
ATGGCAGAAC GGCTGCACGT GAACATTGAG AATTTAAAAT CAGGAATACA AGCCAAAATG 
TCTGGGAATC ATTTGGCTGA AATGTGGCTG AAGTTGATTG ATGAACTTAT CGAAGATACA 
AGCATGCCAT TGCCTTCACT AGAAGGGAAA TCTAATGTCA CCATTCTAGA CACTCAGATC 
CAAAAGCTGC GTCTTCGATC CCTTATCCAG ATTCAAGAAG CAGCTAGAAA AATGAGGTCT 
GAAGCCAAAC ATGTGAAGTC TACTTTGACA GAAATTGAAG ATTGGTTTGA TAAGCTAATG 
CAACTTTCTG AAGAGCCACA AAACAGTATG CCCGATATTA TCATATGGAT GATCCGAGGA 
GAGAAAAGAT TGGCCTATGC TAGAATTCCT GCACACCAGG TTTTATATTC TACTACCAGT 
GAAGAAGCTT CTGGAAAGTA CTGTGGGAAG ACCCAGACCA TCTTTTTGAA GTACCCACAG 
GACAGGAGCT ATGGTGTGAA GGTTCCAGCA GAATTGCGGG TTAACATTTG GCTTGGATTA 
AGTGCTGTTG AAAAGAAGTT TAACAGCTTT GCTGAAGGAA CATTCACTGT CTTTGCAGAA 
ATGTATGAAA ATCAAGCTTT GATGTTTGGA AAATGGGGCA CTTCTGGACT TGTGGGTCGA 
CATAAATTTT CAGATGTCAC AGGCAAGATA AAGCTCAAGA AAGAGTTCTT TTTGCCCCCT 
AAAGGCTGGG AATGGGAAGG TGAATGGATG GTGGATCCTG AAAGAAGTTT ATTGACTGAA 
GCGGATGCTG GCCACACACA CTTTACAGAT GAAGTGTATC AGAATGAAAG CCGCTATCCA 
GGTGGAGAAT GGAAGGCAAT GGAGGACAGC TACACAGATG TGAATGGAGA AAAAGCAGCA 
TCTCCTAATG AGATGACTTG CCCCCCTGGC TGGGAATGGG AAGATGATGC TTGGATGTAT 
GATATAAACC GAGCTGTAGA TGAAAAAGGC TGGGAATATG GAATTACTAT TCCTCCTGAT 
AACAAGCCAA AGTCCTGGGT TGCTGCAGAG AAGATGTATC ATACACACAG GCGGCGGCGA 
ATGGTCCGCA AACGCAAAAA GGATGTGACA CAGTCGGCTG CAAGTACTGC AAGGGGGTGG 
GAAGCACTAG AAGATAAAGA AGGCTGGGAA TATGCATCTT TAATTGGATG GAAATTTCAC 
TGGAAACAGC GTAGTTCAGA TACTTTTCGT CGAAGACGCT GGAGAAGAAA GATGGCTCCT 
TCTGAAACAC ATGGTTCAGC TGCTATCTTT AAACTTGAAG GTGCCATTGG AGCAGACACT 
ACTGAAGATG GAGATGAAAA GAGCACGGAG AAAAACAAAC ATAGTGCAAC AACCGTGTTT 
GGTGCAAACA CTCCAATTGT TTCCTGTAAT TTTGACAGAG TATATAATTA TCATCTTCGC 
TGCTATATAT ATCAAGCTCG AAATCTTATG GCTTTAGATA AAGATAGTTT TTCAGATCCC 
TATGCTCATG TTTCATTCCT CCATCGGAGC CAAACAACTG AAATCATCCA CTCAACACTG 
AATCCAACCT GGGACCAAAC TCTAATATTT GATGACATTG AAATTTTTGG AGAACCACAA 
ACAATCATAC AAAACCCACC CAAAGTTGTC ATTGAACTCT TTGACAATGA CCAAGTGGGC 
AAGGATGAAT TTTTAGGTCG AAGTTTTTGC TCCCCAGTTG TCAAGCTAAA TCCAGAAGTT 
GACATCACCC CTAAGCTTCT CTGGCAACCA GTCATAAATG GAGATAAGGC TTGTGGGGAT 
GTACTTGTAG CTGCTGAGCT TATTCTCAAA GATAAGGTTG GCTCCAATCT TCCAATTCTC 
CCATCTCAGA GGGCTCCAAA TCTCTACATG GTTCCTCAAG GAATTAGGCC TGTGGTTCAA 
CTCACTGCTA TTGAGATTTT AGCTTGGGGT TTAAGAAACA TGAAAAACTA CCAGATGGCT 
TCTGTGACTT CCCCAAGTCT TATCATAGAG TGTGGAGGAG AAAGGATAGA ATCAGCATTG 
ATCAAAAACC TCAAGAAGAC ACCCAACTTT CCTAGTTCTG TTCTCTTCAT GAAAGTGCTA 
TTGCCCAAGG AAGAATTGTA CACACCACCA TTGGTTATCA AAGTGATCGA CCACAGACAA 
TTTGGGCGAA AGCCCATCGT GGGTCAGTGC ACCATCGACC TTCTTGAACA GTTTCGTTGT 
GACCCTTATG CATTTAAGGA AGATATTGCA CCCCAGGTAA AAGCTTCTAT CTTGTCTGCC 
CCACTTAGCC GGGATGTGGT TATTGAAGTT GAGGACACAA AACCATTGCT TCCAACCAAG 
TTCTCGGTAT CTCCATCCTC TTGCCATTTG TGTGGCTCTC TGTCCTGCTG TTGCACACGT 
GTCCATGATC AGTTAACTGA AAAGGAGGAA GAAGTTGTTG ACTGGTGGAG CAAATTTTAT 
GCATCTGTTG GAGAACCTGA AAAGTGTGGG CAATATATCC AGAAGGAATA TGCCAAACTA 
AAGGTATATA ATTGTGAACT AGAGAGTGTT CCAGAGTTTA AGGGTCTGAC AGACTTTTCA 
GATACCTTTA AGCTTTACAG AGGGAAGTCT GATGAGATTG ATGATCCTTC AGTGGTAGGA 
GAGTTCAAGG GCTCCTTTAG AATCTATCCA CTGCCTGATG ATCCAAATGT GCCAGCTCCT 
CCCAGGCAGT TTCGGGAATT ACCTGACAGT GTCCCACAGG AATGCATTGT GAGGATTTAT 
ATTGTTCGGG GCATAGATCT CCAGCCTCAG GATAACAATG GCCTGTGTGA CCCTTATATA 
AAAATATCCC TGAGTAAAAA AGTCATTGAA GATCGAGATA ATTACATTCC AAATACACTC 
AACCCAATTT TTGGAAGGAT GTATGAGCTA AGCTGCTTCT TACCTCAAGA AAAAGACCTG 
AAAATTTCTG TCTATGATTA TGACACACTT ACTCGTGATG AAAAAGTAGG AGAAACTATC 
ATTGATCTAG AAAATCGCTT CCTTTCCCGA TTTGGGTCGA ATTGTGGCAT AGCAGAGCAA 
TATTGCATTT CTGGAGTCAA TACTTGGAGA GATCAACTAA AACCAACCCA ATTGTTATGC 
AATGTAGCTA AGTTTAAAGG ATTGCCACTC CCTATTCTTT CTGAAGATGG AAATAGGCTA 
AGATTTGGAG GTCGAGAGTA CACCTTGGAA GAATTTGAGG CCAACAGAAA TCTGCACCAG 
CACCTGGGAC CTGCAGAGGA GCGTCTTGCT CTGTATATCC TTCGGACTCA GGGTCAAGTC 
CCTGAGCATG TGGAAACAAG GACTTTGTAC AGCACCTTCC AACCAAATAT TTCACAGGGA 
AAACTTCAGA TGTGGGTGGA TGTTTTCCCC AAAAGTTTGG GGCCACCAGG CCCTCCTTTC 
AATATCACTC CCCGGAAGGC CAAGAGGTAT GTCCTACGTG TGATCATTTG GAACACTAAA 
GATGTCATTC TTGATGAGAA AAGTATCACA GGAGAAGAGA TGACTGACAT CTATGTGAAA 
GGCTGGATTC CTGGGAATGA AGAAAACAAG CAGAAGACAG ATGTCCATTA CAGGTCATTG 
GATGGTGAAG GAAACTTTAA TTGGAGATTT GTTTTCCCTT TTGAATACCT GCCTGCTGAA 
CAACTCTGTA TTGTTTCTAA AAAAGAACAT TTCTGGAGTA TTGACCAGAC TGAATTTCGA 
GTTCCTCCCA GACTCATCAT TCAGATATGG GACAATGATA AGTTTTCTTT AGATGACTAC 
TTGGGTTTTC TGGAACTTGA TTTACACAAC ACAATTATCC CTGCAAAGTC TTCAGAAAAG 
TGCAAATTGG ACATGATTTC AGAGTATAGG GCAGTGAATC CTCTAAAGTC CAAGACAGCC 
TCACTTTTTG AACAGAAATC GATGAAAGGA TGGTGGCCAT GCTATGCAGA AAAAGATGGC 
GCACGTGTGA TGACTGGTAA AGTGGAGATG ACTTTGGAAG TCCTCAATGA GAAGGAGGTT 
GATGAAAGAC CAGCTGGGAA AGGAAGGGAT GAGCCCAACA TGAACCCCAA GCTAGAATTA 
CCAAATCGAC CAGAAACTTC ATTCCTTTGG TTTACCAACC CATGCAAGAC AATGAAATTC 
ATCGTGTGGC GTCGATTTAA ATGGGTCATC ATTGGCTTAC TCATCCTGCT GGTCTTGTTG 
CTGTTTTTGG CAGTACTCCT ATATTCTCTG CCGAACTATA TATCAATGAA GATCGTGAGA 
CCAAATGCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016281640.1
CDS	16..6330
Translation	MGVGVFILMILKVSSSYKYYHPIIFQLGVCRGIHERGPKRKEGASKDEDEKKKTKKIDNELNPVWNEIFEFDLKGIPLDFSSTLGITVKDFETIGQNKLIGVATISLKDLIGGQSRSLPYKLISLVNEKGQDTGATIDLVVAYDPPAAMATNPNELSGITIEGTGEEEEEDTGDEDTAFKVIAPRVPATNISEAQLARRITKGKNTRKILSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHMTPLELVDETISIRVYDSHSLRADCLMGEFKIDIGYIYDEPGHAVMRKWLLLNDPEDISSGAKGYMKVSMFVLGTGDEPPTEKRDRDNDSDDVESNLLLPAGIAMRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGANVDKKNLVDPFVEVCFAGKKVSTGIIEKNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEDSSPSGAWAASSTEKTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDDLNSGKGEGVAYRGRILVELNTLLEKTPPDKKVETISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNFYYYLPWAQKKPVVTLTSYWEDISHRLDAVNTILVMAERLHVNIENLKSGIQAKMSGNHLAEMWLKLIDELIEDTSMPLPSLEGKSNVTILDTQIQKLRLRSLIQIQEAARKMRSEAKHVKSTLTEIEDWFDKLMQLSEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTTSEEASGKYCGKTQTIFLKYPQDRSYGVKVPAELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKKEFFLPPKGWEWEGEWMVDPERSLLTEADAGHTHFTDEVYQNESRYPGGEWKAMEDSYTDVNGEKAASPNEMTCPPGWEWEDDAWMYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRMVRKRKKDVTQSAASTARGWEALEDKEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGSAAIFKLEGAIGADTTEDGDEKSTEKNKHSATTVFGANTPIVSCNFDRVYNYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSQTTEIIHSTLNPTWDQTLIFDDIEIFGEPQTIIQNPPKVVIELFDNDQVGKDEFLGRSFCSPVVKLNPEVDITPKLLWQPVINGDKACGDVLVAAELILKDKVGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIIECGGERIESALIKNLKKTPNFPSSVLFMKVLLPKEELYTPPLVIKVIDHRQFGRKPIVGQCTIDLLEQFRCDPYAFKEDIAPQVKASILSAPLSRDVVIEVEDTKPLLPTKFSVSPSSCHLCGSLSCCCTRVHDQLTEKEEEVVDWWSKFYASVGEPEKCGQYIQKEYAKLKVYNCELESVPEFKGLTDFSDTFKLYRGKSDEIDDPSVVGEFKGSFRIYPLPDDPNVPAPPRQFRELPDSVPQECIVRIYIVRGIDLQPQDNNGLCDPYIKISLSKKVIEDRDNYIPNTLNPIFGRMYELSCFLPQEKDLKISVYDYDTLTRDEKVGETIIDLENRFLSRFGSNCGIAEQYCISGVNTWRDQLKPTQLLCNVAKFKGLPLPILSEDGNRLRFGGREYTLEEFEANRNLHQHLGPAEERLALYILRTQGQVPEHVETRTLYSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKRYVLRVIIWNTKDVILDEKSITGEEMTDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFEYLPAEQLCIVSKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHNTIIPAKSSEKCKLDMISEYRAVNPLKSKTASLFEQKSMKGWWPCYAEKDGARVMTGKVEMTLEVLNEKEVDERPAGKGRDEPNMNPKLELPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLILLVLLLFLAVLLYSLPNYISMKIVRPNAK

Target ORF information:

RefSeq Version	XM_016426154.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica myoferlin (MYOF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016426154.1

ORF Insert Sequence:

ATGGGGGTTG GGGTGTTCAT TTTGATGATC TTGAAGGTCT CTTCCAGCTA TAAATACTAT 
CATCCTATTA TCTTTCAGCT GGGGGTATGC AGAGGGATTC ATGAAAGAGG GCCTAAAAGA 
AAGGAAGGAG CTTCAAAAGA TGAAGATGAG AAGAAGAAAA CGAAGAAAAT TGATAATGAA 
CTCAACCCTG TCTGGAATGA GATATTTGAA TTTGATTTGA AGGGCATACC ATTGGACTTT 
TCATCTACTC TTGGCATCAC TGTGAAAGAT TTCGAGACAA TTGGACAAAA TAAACTGATT 
GGCGTTGCAA CAATATCACT GAAGGACTTG ATAGGTGGCC AGAGTCGATC TCTTCCTTAT 
AAACTCATAT CTTTGGTAAA TGAAAAGGGT CAAGATACTG GGGCTACAAT CGATCTGGTG 
GTTGCCTATG ATCCACCAGC AGCCATGGCA ACAAATCCAA ATGAACTAAG TGGGATCACC 
ATAGAAGGCA CGGGAGAAGA AGAGGAAGAA GACACAGGAG ATGAAGATAC TGCATTCAAA 
GTCATTGCCC CAAGGGTTCC AGCAACAAAT ATTTCTGAAG CCCAGCTGGC CAGGAGGATT 
ACAAAAGGAA AGAATACCCG AAAGATCCTT TCCAATAAGC CACAAGACTT CCAGATTCGT 
GTTAGAGTGA TTGAAGGGCG CCAATTAAGT GGGAATAACA TCAAACCAGT GGTCAAAGTC 
CATGTCTGTG GTCAGACACA TCGAACAAGA ATAAAAAGAG GGAATAATCC ATTTTTTGAC 
GAATTATTTT TCTACAATGT CCACATGACC CCTCTTGAAC TGGTTGATGA GACAATAAGC 
ATTCGGGTAT ATGATTCCCA TTCTTTGAGA GCAGACTGTC TGATGGGAGA ATTTAAGATT 
GATATTGGTT ACATTTATGA TGAACCTGGT CATGCTGTCA TGAGAAAATG GCTTCTTCTA 
AATGACCCTG AAGATATAAG TTCTGGTGCT AAAGGTTACA TGAAAGTCAG CATGTTTGTC 
CTTGGAACAG GAGATGAACC ACCAACAGAG AAGAGAGATC GAGATAATGA CAGTGATGAT 
GTGGAGAGTA ATTTGTTACT TCCTGCTGGA ATTGCCATGC GGTGGGTCAC TTTCCTCCTG 
AAAATTTACA GAGCTGAGGA TATTCCTCAG ATGGATGATG CCTTTTCACA GACTGTCAAA 
GAAATATTTG GAGCCAATGT AGACAAGAAA AACTTAGTGG ATCCATTTGT AGAAGTTTGT 
TTTGCTGGGA AAAAGGTTTC CACAGGCATA ATAGAGAAAA ATGCAAATCC AGAGTGGAAT 
CAAGTTGTCA ATCTTCAAAT CAAGTTTCCT TCAATGTGTG AAAAAATAAA GTTAACAGTA 
TATGACTGGG ACCGCCTTAC CAAGAATGAT GTAGTAGGGA CAACCTATCT GTATCTTTCT 
AAAATTGCTG CATCGGGTGG GGAAGTTGAA GATTCCTCAC CTTCGGGAGC TTGGGCTGCA 
TCATCCACAG AGAAAACAGG AGAAACAGAA GTGGGCTTTG TACCAACATT TGGACCTTGT 
TATCTAAATC TTTATGGAAG TCCAAGAGAA TACACTGGAT TCCCAGATCC ATATGATGAC 
TTGAATTCTG GAAAGGGTGA AGGAGTTGCC TACAGAGGTC GAATCTTGGT AGAACTGAAT 
ACTTTACTTG AGAAAACACC CCCTGACAAG AAGGTTGAAA CCATATCCAA TGATGATTTG 
CTGGTTGTTG AGAAATATCA GCGTAGGCGA AAATATAGCC TGTCTGCGGT ATTTCATTCT 
GCAACCATGC TGCAAGATAT TGGAGAGGCC ATTCAGTTTG AAGTCAGCAT TGGAAATTAC 
GGCAATAAGT TTGATGCTAC CTGTAAGCCT TTGGCATCGA CAACTCAATA CAGTCGGGCT 
GTGTTTGATG GAAATTTCTA TTATTATTTG CCATGGGCCC AGAAGAAGCC AGTGGTGACT 
CTAACTTCTT ATTGGGAAGA CATTAGTCAC AGACTGGATG CAGTGAACAC CATTCTTGTT 
ATGGCAGAAC GGCTGCACGT GAACATTGAG AATTTAAAAT CAGGAATACA AGCCAAAATG 
TCTGGGAATC ATTTGGCTGA AATGTGGCTG AAGTTGATTG ATGAACTTAT CGAAGATACA 
AGCATGCCAT TGCCTTCACT AGAAGGGAAA TCTAATGTCA CCATTCTAGA CACTCAGATC 
CAAAAGCTGC GTCTTCGATC CCTTATCCAG ATTCAAGAAG CAGCTAGAAA AATGAGGTCT 
GAAGCCAAAC ATGTGAAGTC TACTTTGACA GAAATTGAAG ATTGGTTTGA TAAGCTAATG 
CAACTTTCTG AAGAGCCACA AAACAGTATG CCCGATATTA TCATATGGAT GATCCGAGGA 
GAGAAAAGAT TGGCCTATGC TAGAATTCCT GCACACCAGG TTTTATATTC TACTACCAGT 
GAAGAAGCTT CTGGAAAGTA CTGTGGGAAG ACCCAGACCA TCTTTTTGAA GTACCCACAG 
GACAGGAGCT ATGGTGTGAA GGTTCCAGCA GAATTGCGGG TTAACATTTG GCTTGGATTA 
AGTGCTGTTG AAAAGAAGTT TAACAGCTTT GCTGAAGGAA CATTCACTGT CTTTGCAGAA 
ATGTATGAAA ATCAAGCTTT GATGTTTGGA AAATGGGGCA CTTCTGGACT TGTGGGTCGA 
CATAAATTTT CAGATGTCAC AGGCAAGATA AAGCTCAAGA AAGAGTTCTT TTTGCCCCCT 
AAAGGCTGGG AATGGGAAGG TGAATGGATG GTGGATCCTG AAAGAAGTTT ATTGACTGAA 
GCGGATGCTG GCCACACACA CTTTACAGAT GAAGTGTATC AGAATGAAAG CCGCTATCCA 
GGTGGAGAAT GGAAGGCAAT GGAGGACAGC TACACAGATG TGAATGGAGA AAAAGCAGCA 
TCTCCTAATG AGATGACTTG CCCCCCTGGC TGGGAATGGG AAGATGATGC TTGGATGTAT 
GATATAAACC GAGCTGTAGA TGAAAAAGGC TGGGAATATG GAATTACTAT TCCTCCTGAT 
AACAAGCCAA AGTCCTGGGT TGCTGCAGAG AAGATGTATC ATACACACAG GCGGCGGCGA 
ATGGTCCGCA AACGCAAAAA GGATGTGACA CAGTCGGCTG CAAGTACTGC AAGGGGGTGG 
GAAGCACTAG AAGATAAAGA AGGCTGGGAA TATGCATCTT TAATTGGATG GAAATTTCAC 
TGGAAACAGC GTAGTTCAGA TACTTTTCGT CGAAGACGCT GGAGAAGAAA GATGGCTCCT 
TCTGAAACAC ATGGTTCAGC TGCTATCTTT AAACTTGAAG GTGCCATTGG AGCAGACACT 
ACTGAAGATG GAGATGAAAA GAGCACGGAG AAAAACAAAC ATAGTGCAAC AACCGTGTTT 
GGTGCAAACA CTCCAATTGT TTCCTGTAAT TTTGACAGAG TATATAATTA TCATCTTCGC 
TGCTATATAT ATCAAGCTCG AAATCTTATG GCTTTAGATA AAGATAGTTT TTCAGATCCC 
TATGCTCATG TTTCATTCCT CCATCGGAGC CAAACAACTG AAATCATCCA CTCAACACTG 
AATCCAACCT GGGACCAAAC TCTAATATTT GATGACATTG AAATTTTTGG AGAACCACAA 
ACAATCATAC AAAACCCACC CAAAGTTGTC ATTGAACTCT TTGACAATGA CCAAGTGGGC 
AAGGATGAAT TTTTAGGTCG AAGTTTTTGC TCCCCAGTTG TCAAGCTAAA TCCAGAAGTT 
GACATCACCC CTAAGCTTCT CTGGCAACCA GTCATAAATG GAGATAAGGC TTGTGGGGAT 
GTACTTGTAG CTGCTGAGCT TATTCTCAAA GATAAGGTTG GCTCCAATCT TCCAATTCTC 
CCATCTCAGA GGGCTCCAAA TCTCTACATG GTTCCTCAAG GAATTAGGCC TGTGGTTCAA 
CTCACTGCTA TTGAGATTTT AGCTTGGGGT TTAAGAAACA TGAAAAACTA CCAGATGGCT 
TCTGTGACTT CCCCAAGTCT TATCATAGAG TGTGGAGGAG AAAGGATAGA ATCAGCATTG 
ATCAAAAACC TCAAGAAGAC ACCCAACTTT CCTAGTTCTG TTCTCTTCAT GAAAGTGCTA 
TTGCCCAAGG AAGAATTGTA CACACCACCA TTGGTTATCA AAGTGATCGA CCACAGACAA 
TTTGGGCGAA AGCCCATCGT GGGTCAGTGC ACCATCGACC TTCTTGAACA GTTTCGTTGT 
GACCCTTATG CATTTAAGGA AGATATTGCA CCCCAGGTAA AAGCTTCTAT CTTGTCTGCC 
CCACTTAGCC GGGATGTGGT TATTGAAGTT GAGGACACAA AACCATTGCT TCCAACCAAG 
TTCTCGGTAT CTCCATCCTC TTGCCATTTG TGTGGCTCTC TGTCCTGCTG TTGCACACGT 
GTCCATGATC AGTTAACTGA AAAGGAGGAA GAAGTTGTTG ACTGGTGGAG CAAATTTTAT 
GCATCTGTTG GAGAACCTGA AAAGTGTGGG CAATATATCC AGAAGGAATA TGCCAAACTA 
AAGGTATATA ATTGTGAACT AGAGAGTGTT CCAGAGTTTA AGGGTCTGAC AGACTTTTCA 
GATACCTTTA AGCTTTACAG AGGGAAGTCT GATGAGATTG ATGATCCTTC AGTGGTAGGA 
GAGTTCAAGG GCTCCTTTAG AATCTATCCA CTGCCTGATG ATCCAAATGT GCCAGCTCCT 
CCCAGGCAGT TTCGGGAATT ACCTGACAGT GTCCCACAGG AATGCATTGT GAGGATTTAT 
ATTGTTCGGG GCATAGATCT CCAGCCTCAG GATAACAATG GCCTGTGTGA CCCTTATATA 
AAAATATCCC TGAGTAAAAA AGTCATTGAA GATCGAGATA ATTACATTCC AAATACACTC 
AACCCAATTT TTGGAAGGAT GTATGAGCTA AGCTGCTTCT TACCTCAAGA AAAAGACCTG 
AAAATTTCTG TCTATGATTA TGACACACTT ACTCGTGATG AAAAAGTAGG AGAAACTATC 
ATTGATCTAG AAAATCGCTT CCTTTCCCGA TTTGGGTCGA ATTGTGGCAT AGCAGAGCAA 
TATTGCATTT CTGGAGTCAA TACTTGGAGA GATCAACTAA AACCAACCCA ATTGTTATGC 
AATGTAGCTA AGTTTAAAGG ATTGCCACTC CCTATTCTTT CTGAAGATGG AAATAGGCTA 
AGATTTGGAG GTCGAGAGTA CACCTTGGAA GAATTTGAGG CCAACAGAAA TCTGCACCAG 
CACCTGGGAC CTGCAGAGGA GCGTCTTGCT CTGTATATCC TTCGGACTCA GGGTCAAGTC 
CCTGAGCATG TGGAAACAAG GACTTTGTAC AGCACCTTCC AACCAAATAT TTCACAGGGA 
AAACTTCAGA TGTGGGTGGA TGTTTTCCCC AAAAGTTTGG GGCCACCAGG CCCTCCTTTC 
AATATCACTC CCCGGAAGGC CAAGAGGTAT GTCCTACGTG TGATCATTTG GAACACTAAA 
GATGTCATTC TTGATGAGAA AAGTATCACA GGAGAAGAGA TGACTGACAT CTATGTGAAA 
GGCTGGATTC CTGGGAATGA AGAAAACAAG CAGAAGACAG ATGTCCATTA CAGGTCATTG 
GATGGTGAAG GAAACTTTAA TTGGAGATTT GTTTTCCCTT TTGAATACCT GCCTGCTGAA 
CAACTCTGTA TTGTTTCTAA AAAAGAACAT TTCTGGAGTA TTGACCAGAC TGAATTTCGA 
GTTCCTCCCA GACTCATCAT TCAGATATGG GACAATGATA AGTTTTCTTT AGATGACTAC 
TTGGGTTTTC TGGAACTTGA TTTACACAAC ACAATTATCC CTGCAAAGTC TTCAGAAAAG 
TGCAAATTGG ACATGATTTC AGAGTATAGG GCAGTGAATC CTCTAAAGTC CAAGACAGCC 
TCACTTTTTG AACAGAAATC GATGAAAGGA TGGTGGCCAT GCTATGCAGA AAAAGATGGC 
GCACGTGTGA TGACTGGTAA AGTGGAGATG ACTTTGGAAG TCCTCAATGA GAAGGAGGTT 
GATGAAAGAC CAGCTGGGAA AGGAAGGGAT GAGCCCAACA TGAACCCCAA GCTAGAATTA 
CCAAATCGAC CAGAAACTTC ATTCCTTTGG TTTACCAACC CATGCAAGAC AATGAAATTC 
ATCGTGTGGC GTCGATTTAA ATGGGTCATC ATTGGCTTAC TCATCCTGCT GGTCTTGTTG 
CTGTTTTTGG CAGTACTCCT ATATTCTCTG CCGAACTATA TATCAATGAA GATCGTGAGA 
CCAAATGCAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_007478794.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007478856.2 Monodelphis domestica myoferlin (MYOF), transcript variant X2, mRNA.

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CloneID	OMc38445
Clone ID Related Accession (Same CDS sequence)	XM_007478794.2
Accession Version	XM_007478794.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	myoferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007478794.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTTG GGGTGTTCAT TTTGATGATC TTGAAGGTCT CTTCCAGCTA TAAATACTAT 
CATCCTATTA TCTTTCAGCT GGGGGTATGC AGAGGGATTC ATGAAAGAGG GCCTAAAAGA 
AAGGAAGGAG CTTCAAAAGA TGAAGATGAG AAGAAGAAAA CGAAGAAAAT TGATAATGAA 
CTCAACCCTG TCTGGAATGA GATATTTGAA TTTGATTTGA AGGGCATACC ATTGGACTTT 
TCATCTACTC TTGGCATCAC TGTGAAAGAT TTCGAGACAA TTGGACAAAA TAAACTGATT 
GGCGTTGCAA CAATATCACT GAAGGACTTG ATAGGTGGCC AGAGTCGATC TCTTCCTTAT 
AAACTCATAT CTTTGGTAAA TGAAAAGGGT CAAGATACTG GGGCTACAAT CGATCTGGTG 
GTTGCCTATG ATCCACCAGC AGCCATGGCA ACAAATCCAA ATGAACTAAG TGGGATCACC 
ATAGAAGGCA CGGGAGAAGA AGAGGAAGAA GACACAGGAG ATGAAGATAC TGCATTCAAA 
GTCATTGCCC CAAGGGTTCC AGCAACAAAT ATTTCTGAAG CCCAGCTGGC CAGGAGGATT 
ACAAAAGGAA AGAATACCCG AAAGATCCTT TCCAATAAGC CACAAGACTT CCAGATTCGT 
GTTAGAGTGA TTGAAGGGCG CCAATTAAGT GGGAATAACA TCAAACCAGT GGTCAAAGTC 
CATGTCTGTG GTCAGACACA TCGAACAAGA ATAAAAAGAG GGAATAATCC ATTTTTTGAC 
GAATTATTTT TCTACAATGT CCACATGACC CCTCTTGAAC TGGTTGATGA GACAATAAGC 
ATTCGGGTAT ATGATTCCCA TTCTTTGAGA GCAGACTGTC TGATGGGAGA ATTTAAGATT 
GATATTGGTT ACATTTATGA TGAACCTGGT CATGCTGTCA TGAGAAAATG GCTTCTTCTA 
AATGACCCTG AAGATATAAG TTCTGGTGCT AAAGGTTACA TGAAAGTCAG CATGTTTGTC 
CTTGGAACAG GAGATGAACC ACCAACAGAG AAGAGAGATC GAGATAATGA CAGTGATGAT 
GTGGAGAGTA ATTTGTTACT TCCTGCTGGA ATTGCCATGC GGTGGGTCAC TTTCCTCCTG 
AAAATTTACA GAGCTGAGGA TATTCCTCAG ATGGATGATG CCTTTTCACA GACTGTCAAA 
GAAATATTTG GAGCCAATGT AGACAAGAAA AACTTAGTGG ATCCATTTGT AGAAGTTTGT 
TTTGCTGGGA AAAAGGTTTC CACAGGCATA ATAGAGAAAA ATGCAAATCC AGAGTGGAAT 
CAAGTTGTCA ATCTTCAAAT CAAGTTTCCT TCAATGTGTG AAAAAATAAA GTTAACAGTA 
TATGACTGGG ACCGCCTTAC CAAGAATGAT GTAGTAGGGA CAACCTATCT GTATCTTTCT 
AAAATTGCTG CATCGGGTGG GGAAGTTGAA GATTCCTCAC CTTCGGGAGC TTGGGCTGCA 
TCATCCACAG AGAAAACAGG AGAAACAGAA GTGGGCTTTG TACCAACATT TGGACCTTGT 
TATCTAAATC TTTATGGAAG TCCAAGAGAA TACACTGGAT TCCCAGATCC ATATGATGAC 
TTGAATTCTG GAAAGGGTGA AGGAGTTGCC TACAGAGGTC GAATCTTGGT AGAACTGAAT 
ACTTTACTTG AGAAAACACC CCCTGACAAG AAGGTTGAAA CCATATCCAA TGATGATTTG 
CTGGTTGTTG AGAAATATCA GCGTAGGCGA AAATATAGCC TGTCTGCGGT ATTTCATTCT 
GCAACCATGC TGCAAGATAT TGGAGAGGCC ATTCAGTTTG AAGTCAGCAT TGGAAATTAC 
GGCAATAAGT TTGATGCTAC CTGTAAGCCT TTGGCATCGA CAACTCAATA CAGTCGGGCT 
GTGTTTGATG GAAATTTCTA TTATTATTTG CCATGGGCCC AGAAGAAGCC AGTGGTGACT 
CTAACTTCTT ATTGGGAAGA CATTAGTCAC AGACTGGATG CAGTGAACAC CATTCTTGTT 
ATGGCAGAAC GGCTGCACGT GAACATTGAG AATTTAAAAT CAGGAATACA AGCCAAAATG 
TCTGGGAATC ATTTGGCTGA AATGTGGCTG AAGTTGATTG ATGAACTTAT CGAAGATACA 
AGCATGCCAT TGCCTTCACT AGAAGGGAAA TCTAATGTCA CCATTCTAGA CACTCAGATC 
CAAAAGCTGC GTCTTCGATC CCTTATCCAG ATTCAAGAAG CAGCTAGAAA AATGAGGTCT 
GAAGCCAAAC ATGTGAAGTC TACTTTGACA GAAATTGAAG ATTGGTTTGA TAAGCTAATG 
CAACTTTCTG AAGAGCCACA AAACAGTATG CCCGATATTA TCATATGGAT GATCCGAGGA 
GAGAAAAGAT TGGCCTATGC TAGAATTCCT GCACACCAGG TTTTATATTC TACTACCAGT 
GAAGAAGCTT CTGGAAAGTA CTGTGGGAAG ACCCAGACCA TCTTTTTGAA GTACCCACAG 
GACAGGAGCT ATGGTGTGAA GGTTCCAGCA GAATTGCGGG TTAACATTTG GCTTGGATTA 
AGTGCTGTTG AAAAGAAGTT TAACAGCTTT GCTGAAGGAA CATTCACTGT CTTTGCAGAA 
ATGTATGAAA ATCAAGCTTT GATGTTTGGA AAATGGGGCA CTTCTGGACT TGTGGGTCGA 
CATAAATTTT CAGATGTCAC AGGCAAGATA AAGCTCAAGA AAGAGTTCTT TTTGCCCCCT 
AAAGGCTGGG AATGGGAAGG TGAATGGATG GTGGATCCTG AAAGAAGTTT ATTGACTGAA 
GCGGATGCTG GCCACACACA CTTTACAGAT GAAGTGTATC AGAATGAAAG CCGCTATCCA 
GGTGGAGAAT GGAAGGCAAT GGAGGACAGC TACACAGATG TGAATGGAGA AAAAGCAGCA 
TCTCCTAATG AGATGACTTG CCCCCCTGGC TGGGAATGGG AAGATGATGC TTGGATGTAT 
GATATAAACC GAGCTGTAGA TGAAAAAGGC TGGGAATATG GAATTACTAT TCCTCCTGAT 
AACAAGCCAA AGTCCTGGGT TGCTGCAGAG AAGATGTATC ATACACACAG GCGGCGGCGA 
ATGGTCCGCA AACGCAAAAA GGATGTGACA CAGTCGGCTG CAAGTACTGC AAGGGGGTGG 
GAAGCACTAG AAGATAAAGA AGGCTGGGAA TATGCATCTT TAATTGGATG GAAATTTCAC 
TGGAAACAGC GTAGTTCAGA TACTTTTCGT CGAAGACGCT GGAGAAGAAA GATGGCTCCT 
TCTGAAACAC ATGGTTCAGC TGCTATCTTT AAACTTGAAG GTGCCATTGG AGCAGACACT 
ACTGAAGATG GAGATGAAAA GAGCACGGAG AAAAACAAAC ATAGTGCAAC AACCGTGTTT 
GGTGCAAACA CTCCAATTGT TTCCTGTAAT TTTGACAGAG TATATAATTA TCATCTTCGC 
TGCTATATAT ATCAAGCTCG AAATCTTATG GCTTTAGATA AAGATAGTTT TTCAGATCCC 
TATGCTCATG TTTCATTCCT CCATCGGAGC CAAACAACTG AAATCATCCA CTCAACACTG 
AATCCAACCT GGGACCAAAC TCTAATATTT GATGACATTG AAATTTTTGG AGAACCACAA 
ACAATCATAC AAAACCCACC CAAAGTTGTC ATTGAACTCT TTGACAATGA CCAAGTGGGC 
AAGGATGAAT TTTTAGGTCG AAGTTTTTGC TCCCCAGTTG TCAAGCTAAA TCCAGAAGTT 
GACATCACCC CTAAGCTTCT CTGGCAACCA GTCATAAATG GAGATAAGGC TTGTGGGGAT 
GTACTTGTAG CTGCTGAGCT TATTCTCAAA GATAAGGTTG GCTCCAATCT TCCAATTCTC 
CCATCTCAGA GGGCTCCAAA TCTCTACATG GTTCCTCAAG GAATTAGGCC TGTGGTTCAA 
CTCACTGCTA TTGAGATTTT AGCTTGGGGT TTAAGAAACA TGAAAAACTA CCAGATGGCT 
TCTGTGACTT CCCCAAGTCT TATCATAGAG TGTGGAGGAG AAAGGATAGA ATCAGCATTG 
ATCAAAAACC TCAAGAAGAC ACCCAACTTT CCTAGTTCTG TTCTCTTCAT GAAAGTGCTA 
TTGCCCAAGG AAGAATTGTA CACACCACCA TTGGTTATCA AAGTGATCGA CCACAGACAA 
TTTGGGCGAA AGCCCATCGT GGGTCAGTGC ACCATCGACC TTCTTGAACA GTTTCGTTGT 
GACCCTTATG CATTTAAGGA AGATATTGCA CCCCAGGTAA AAGCTTCTAT CTTGTCTGCC 
CCACTTAGCC GGGATGTGGT TATTGAAGTT GAGGACACAA AACCATTGCT TCCAACCAAG 
TGTTTAAGCA GCATGTCCAC AGCAATCAGC AAAATGACTC CTCCGAGAAC AGTGCATTTA 
ACTGAAAAGG AGGAAGAAGT TGTTGACTGG TGGAGCAAAT TTTATGCATC TGTTGGAGAA 
CCTGAAAAGT GTGGGCAATA TATCCAGAAG GAATATGCCA AACTAAAGGT ATATAATTGT 
GAACTAGAGA GTGTTCCAGA GTTTAAGGGT CTGACAGACT TTTCAGATAC CTTTAAGCTT 
TACAGAGGGA AGTCTGATGA GATTGATGAT CCTTCAGTGG TAGGAGAGTT CAAGGGCTCC 
TTTAGAATCT ATCCACTGCC TGATGATCCA AATGTGCCAG CTCCTCCCAG GCAGTTTCGG 
GAATTACCTG ACAGTGTCCC ACAGGAATGC ATTGTGAGGA TTTATATTGT TCGGGGCATA 
GATCTCCAGC CTCAGGATAA CAATGGCCTG TGTGACCCTT ATATAAAAAT ATCCCTGAGT 
AAAAAAGTCA TTGAAGATCG AGATAATTAC ATTCCAAATA CACTCAACCC AATTTTTGGA 
AGGATGTATG AGCTAAGCTG CTTCTTACCT CAAGAAAAAG ACCTGAAAAT TTCTGTCTAT 
GATTATGACA CACTTACTCG TGATGAAAAA GTAGGAGAAA CTATCATTGA TCTAGAAAAT 
CGCTTCCTTT CCCGATTTGG GTCGAATTGT GGCATAGCAG AGCAATATTG CATTTCTGGA 
GTCAATACTT GGAGAGATCA ACTAAAACCA ACCCAATTGT TATGCAATGT AGCTAAGTTT 
AAAGGATTGC CACTCCCTAT TCTTTCTGAA GATGGAAATA GGCTAAGATT TGGAGGTCGA 
GAGTACACCT TGGAAGAATT TGAGGCCAAC AGAAATCTGC ACCAGCACCT GGGACCTGCA 
GAGGAGCGTC TTGCTCTGTA TATCCTTCGG ACTCAGGGTC AAGTCCCTGA GCATGTGGAA 
ACAAGGACTT TGTACAGCAC CTTCCAACCA AATATTTCAC AGGGAAAACT TCAGATGTGG 
GTGGATGTTT TCCCCAAAAG TTTGGGGCCA CCAGGCCCTC CTTTCAATAT CACTCCCCGG 
AAGGCCAAGA GGTATGTCCT ACGTGTGATC ATTTGGAACA CTAAAGATGT CATTCTTGAT 
GAGAAAAGTA TCACAGGAGA AGAGATGACT GACATCTATG TGAAAGGCTG GATTCCTGGG 
AATGAAGAAA ACAAGCAGAA GACAGATGTC CATTACAGGT CATTGGATGG TGAAGGAAAC 
TTTAATTGGA GATTTGTTTT CCCTTTTGAA TACCTGCCTG CTGAACAACT CTGTATTGTT 
TCTAAAAAAG AACATTTCTG GAGTATTGAC CAGACTGAAT TTCGAGTTCC TCCCAGACTC 
ATCATTCAGA TATGGGACAA TGATAAGTTT TCTTTAGATG ACTACTTGGG TTTTCTGGAA 
CTTGATTTAC ACAACACAAT TATCCCTGCA AAGTCTTCAG AAAAGTGCAA ATTGGACATG 
ATTTCAGAGT ATAGGGCAGT GAATCCTCTA AAGTCCAAGA CAGCCTCACT TTTTGAACAG 
AAATCGATGA AAGGATGGTG GCCATGCTAT GCAGAAAAAG ATGGCGCACG TGTGATGACT 
GGTAAAGTGG AGATGACTTT GGAAGTCCTC AATGAGAAGG AGGTTGATGA AAGACCAGCT 
GGGAAAGGAA GGGATGAGCC CAACATGAAC CCCAAGCTAG AATTACCAAA TCGACCAGAA 
ACTTCATTCC TTTGGTTTAC CAACCCATGC AAGACAATGA AATTCATCGT GTGGCGTCGA 
TTTAAATGGG TCATCATTGG CTTACTCATC CTGCTGGTCT TGTTGCTGTT TTTGGCAGTA 
CTCCTATATT CTCTGCCGAA CTATATATCA ATGAAGATCG TGAGACCAAA TGCAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007478856.2
CDS	16..6315
Translation	MGVGVFILMILKVSSSYKYYHPIIFQLGVCRGIHERGPKRKEGASKDEDEKKKTKKIDNELNPVWNEIFEFDLKGIPLDFSSTLGITVKDFETIGQNKLIGVATISLKDLIGGQSRSLPYKLISLVNEKGQDTGATIDLVVAYDPPAAMATNPNELSGITIEGTGEEEEEDTGDEDTAFKVIAPRVPATNISEAQLARRITKGKNTRKILSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHMTPLELVDETISIRVYDSHSLRADCLMGEFKIDIGYIYDEPGHAVMRKWLLLNDPEDISSGAKGYMKVSMFVLGTGDEPPTEKRDRDNDSDDVESNLLLPAGIAMRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGANVDKKNLVDPFVEVCFAGKKVSTGIIEKNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEDSSPSGAWAASSTEKTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDDLNSGKGEGVAYRGRILVELNTLLEKTPPDKKVETISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNFYYYLPWAQKKPVVTLTSYWEDISHRLDAVNTILVMAERLHVNIENLKSGIQAKMSGNHLAEMWLKLIDELIEDTSMPLPSLEGKSNVTILDTQIQKLRLRSLIQIQEAARKMRSEAKHVKSTLTEIEDWFDKLMQLSEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTTSEEASGKYCGKTQTIFLKYPQDRSYGVKVPAELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKKEFFLPPKGWEWEGEWMVDPERSLLTEADAGHTHFTDEVYQNESRYPGGEWKAMEDSYTDVNGEKAASPNEMTCPPGWEWEDDAWMYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRMVRKRKKDVTQSAASTARGWEALEDKEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGSAAIFKLEGAIGADTTEDGDEKSTEKNKHSATTVFGANTPIVSCNFDRVYNYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSQTTEIIHSTLNPTWDQTLIFDDIEIFGEPQTIIQNPPKVVIELFDNDQVGKDEFLGRSFCSPVVKLNPEVDITPKLLWQPVINGDKACGDVLVAAELILKDKVGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIIECGGERIESALIKNLKKTPNFPSSVLFMKVLLPKEELYTPPLVIKVIDHRQFGRKPIVGQCTIDLLEQFRCDPYAFKEDIAPQVKASILSAPLSRDVVIEVEDTKPLLPTKCLSSMSTAISKMTPPRTVHLTEKEEEVVDWWSKFYASVGEPEKCGQYIQKEYAKLKVYNCELESVPEFKGLTDFSDTFKLYRGKSDEIDDPSVVGEFKGSFRIYPLPDDPNVPAPPRQFRELPDSVPQECIVRIYIVRGIDLQPQDNNGLCDPYIKISLSKKVIEDRDNYIPNTLNPIFGRMYELSCFLPQEKDLKISVYDYDTLTRDEKVGETIIDLENRFLSRFGSNCGIAEQYCISGVNTWRDQLKPTQLLCNVAKFKGLPLPILSEDGNRLRFGGREYTLEEFEANRNLHQHLGPAEERLALYILRTQGQVPEHVETRTLYSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKRYVLRVIIWNTKDVILDEKSITGEEMTDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFEYLPAEQLCIVSKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHNTIIPAKSSEKCKLDMISEYRAVNPLKSKTASLFEQKSMKGWWPCYAEKDGARVMTGKVEMTLEVLNEKEVDERPAGKGRDEPNMNPKLELPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLILLVLLLFLAVLLYSLPNYISMKIVRPNAK

Target ORF information:

RefSeq Version	XM_007478794.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica myoferlin (MYOF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007478794.2

ORF Insert Sequence:

ATGGGGGTTG GGGTGTTCAT TTTGATGATC TTGAAGGTCT CTTCCAGCTA TAAATACTAT 
CATCCTATTA TCTTTCAGCT GGGGGTATGC AGAGGGATTC ATGAAAGAGG GCCTAAAAGA 
AAGGAAGGAG CTTCAAAAGA TGAAGATGAG AAGAAGAAAA CGAAGAAAAT TGATAATGAA 
CTCAACCCTG TCTGGAATGA GATATTTGAA TTTGATTTGA AGGGCATACC ATTGGACTTT 
TCATCTACTC TTGGCATCAC TGTGAAAGAT TTCGAGACAA TTGGACAAAA TAAACTGATT 
GGCGTTGCAA CAATATCACT GAAGGACTTG ATAGGTGGCC AGAGTCGATC TCTTCCTTAT 
AAACTCATAT CTTTGGTAAA TGAAAAGGGT CAAGATACTG GGGCTACAAT CGATCTGGTG 
GTTGCCTATG ATCCACCAGC AGCCATGGCA ACAAATCCAA ATGAACTAAG TGGGATCACC 
ATAGAAGGCA CGGGAGAAGA AGAGGAAGAA GACACAGGAG ATGAAGATAC TGCATTCAAA 
GTCATTGCCC CAAGGGTTCC AGCAACAAAT ATTTCTGAAG CCCAGCTGGC CAGGAGGATT 
ACAAAAGGAA AGAATACCCG AAAGATCCTT TCCAATAAGC CACAAGACTT CCAGATTCGT 
GTTAGAGTGA TTGAAGGGCG CCAATTAAGT GGGAATAACA TCAAACCAGT GGTCAAAGTC 
CATGTCTGTG GTCAGACACA TCGAACAAGA ATAAAAAGAG GGAATAATCC ATTTTTTGAC 
GAATTATTTT TCTACAATGT CCACATGACC CCTCTTGAAC TGGTTGATGA GACAATAAGC 
ATTCGGGTAT ATGATTCCCA TTCTTTGAGA GCAGACTGTC TGATGGGAGA ATTTAAGATT 
GATATTGGTT ACATTTATGA TGAACCTGGT CATGCTGTCA TGAGAAAATG GCTTCTTCTA 
AATGACCCTG AAGATATAAG TTCTGGTGCT AAAGGTTACA TGAAAGTCAG CATGTTTGTC 
CTTGGAACAG GAGATGAACC ACCAACAGAG AAGAGAGATC GAGATAATGA CAGTGATGAT 
GTGGAGAGTA ATTTGTTACT TCCTGCTGGA ATTGCCATGC GGTGGGTCAC TTTCCTCCTG 
AAAATTTACA GAGCTGAGGA TATTCCTCAG ATGGATGATG CCTTTTCACA GACTGTCAAA 
GAAATATTTG GAGCCAATGT AGACAAGAAA AACTTAGTGG ATCCATTTGT AGAAGTTTGT 
TTTGCTGGGA AAAAGGTTTC CACAGGCATA ATAGAGAAAA ATGCAAATCC AGAGTGGAAT 
CAAGTTGTCA ATCTTCAAAT CAAGTTTCCT TCAATGTGTG AAAAAATAAA GTTAACAGTA 
TATGACTGGG ACCGCCTTAC CAAGAATGAT GTAGTAGGGA CAACCTATCT GTATCTTTCT 
AAAATTGCTG CATCGGGTGG GGAAGTTGAA GATTCCTCAC CTTCGGGAGC TTGGGCTGCA 
TCATCCACAG AGAAAACAGG AGAAACAGAA GTGGGCTTTG TACCAACATT TGGACCTTGT 
TATCTAAATC TTTATGGAAG TCCAAGAGAA TACACTGGAT TCCCAGATCC ATATGATGAC 
TTGAATTCTG GAAAGGGTGA AGGAGTTGCC TACAGAGGTC GAATCTTGGT AGAACTGAAT 
ACTTTACTTG AGAAAACACC CCCTGACAAG AAGGTTGAAA CCATATCCAA TGATGATTTG 
CTGGTTGTTG AGAAATATCA GCGTAGGCGA AAATATAGCC TGTCTGCGGT ATTTCATTCT 
GCAACCATGC TGCAAGATAT TGGAGAGGCC ATTCAGTTTG AAGTCAGCAT TGGAAATTAC 
GGCAATAAGT TTGATGCTAC CTGTAAGCCT TTGGCATCGA CAACTCAATA CAGTCGGGCT 
GTGTTTGATG GAAATTTCTA TTATTATTTG CCATGGGCCC AGAAGAAGCC AGTGGTGACT 
CTAACTTCTT ATTGGGAAGA CATTAGTCAC AGACTGGATG CAGTGAACAC CATTCTTGTT 
ATGGCAGAAC GGCTGCACGT GAACATTGAG AATTTAAAAT CAGGAATACA AGCCAAAATG 
TCTGGGAATC ATTTGGCTGA AATGTGGCTG AAGTTGATTG ATGAACTTAT CGAAGATACA 
AGCATGCCAT TGCCTTCACT AGAAGGGAAA TCTAATGTCA CCATTCTAGA CACTCAGATC 
CAAAAGCTGC GTCTTCGATC CCTTATCCAG ATTCAAGAAG CAGCTAGAAA AATGAGGTCT 
GAAGCCAAAC ATGTGAAGTC TACTTTGACA GAAATTGAAG ATTGGTTTGA TAAGCTAATG 
CAACTTTCTG AAGAGCCACA AAACAGTATG CCCGATATTA TCATATGGAT GATCCGAGGA 
GAGAAAAGAT TGGCCTATGC TAGAATTCCT GCACACCAGG TTTTATATTC TACTACCAGT 
GAAGAAGCTT CTGGAAAGTA CTGTGGGAAG ACCCAGACCA TCTTTTTGAA GTACCCACAG 
GACAGGAGCT ATGGTGTGAA GGTTCCAGCA GAATTGCGGG TTAACATTTG GCTTGGATTA 
AGTGCTGTTG AAAAGAAGTT TAACAGCTTT GCTGAAGGAA CATTCACTGT CTTTGCAGAA 
ATGTATGAAA ATCAAGCTTT GATGTTTGGA AAATGGGGCA CTTCTGGACT TGTGGGTCGA 
CATAAATTTT CAGATGTCAC AGGCAAGATA AAGCTCAAGA AAGAGTTCTT TTTGCCCCCT 
AAAGGCTGGG AATGGGAAGG TGAATGGATG GTGGATCCTG AAAGAAGTTT ATTGACTGAA 
GCGGATGCTG GCCACACACA CTTTACAGAT GAAGTGTATC AGAATGAAAG CCGCTATCCA 
GGTGGAGAAT GGAAGGCAAT GGAGGACAGC TACACAGATG TGAATGGAGA AAAAGCAGCA 
TCTCCTAATG AGATGACTTG CCCCCCTGGC TGGGAATGGG AAGATGATGC TTGGATGTAT 
GATATAAACC GAGCTGTAGA TGAAAAAGGC TGGGAATATG GAATTACTAT TCCTCCTGAT 
AACAAGCCAA AGTCCTGGGT TGCTGCAGAG AAGATGTATC ATACACACAG GCGGCGGCGA 
ATGGTCCGCA AACGCAAAAA GGATGTGACA CAGTCGGCTG CAAGTACTGC AAGGGGGTGG 
GAAGCACTAG AAGATAAAGA AGGCTGGGAA TATGCATCTT TAATTGGATG GAAATTTCAC 
TGGAAACAGC GTAGTTCAGA TACTTTTCGT CGAAGACGCT GGAGAAGAAA GATGGCTCCT 
TCTGAAACAC ATGGTTCAGC TGCTATCTTT AAACTTGAAG GTGCCATTGG AGCAGACACT 
ACTGAAGATG GAGATGAAAA GAGCACGGAG AAAAACAAAC ATAGTGCAAC AACCGTGTTT 
GGTGCAAACA CTCCAATTGT TTCCTGTAAT TTTGACAGAG TATATAATTA TCATCTTCGC 
TGCTATATAT ATCAAGCTCG AAATCTTATG GCTTTAGATA AAGATAGTTT TTCAGATCCC 
TATGCTCATG TTTCATTCCT CCATCGGAGC CAAACAACTG AAATCATCCA CTCAACACTG 
AATCCAACCT GGGACCAAAC TCTAATATTT GATGACATTG AAATTTTTGG AGAACCACAA 
ACAATCATAC AAAACCCACC CAAAGTTGTC ATTGAACTCT TTGACAATGA CCAAGTGGGC 
AAGGATGAAT TTTTAGGTCG AAGTTTTTGC TCCCCAGTTG TCAAGCTAAA TCCAGAAGTT 
GACATCACCC CTAAGCTTCT CTGGCAACCA GTCATAAATG GAGATAAGGC TTGTGGGGAT 
GTACTTGTAG CTGCTGAGCT TATTCTCAAA GATAAGGTTG GCTCCAATCT TCCAATTCTC 
CCATCTCAGA GGGCTCCAAA TCTCTACATG GTTCCTCAAG GAATTAGGCC TGTGGTTCAA 
CTCACTGCTA TTGAGATTTT AGCTTGGGGT TTAAGAAACA TGAAAAACTA CCAGATGGCT 
TCTGTGACTT CCCCAAGTCT TATCATAGAG TGTGGAGGAG AAAGGATAGA ATCAGCATTG 
ATCAAAAACC TCAAGAAGAC ACCCAACTTT CCTAGTTCTG TTCTCTTCAT GAAAGTGCTA 
TTGCCCAAGG AAGAATTGTA CACACCACCA TTGGTTATCA AAGTGATCGA CCACAGACAA 
TTTGGGCGAA AGCCCATCGT GGGTCAGTGC ACCATCGACC TTCTTGAACA GTTTCGTTGT 
GACCCTTATG CATTTAAGGA AGATATTGCA CCCCAGGTAA AAGCTTCTAT CTTGTCTGCC 
CCACTTAGCC GGGATGTGGT TATTGAAGTT GAGGACACAA AACCATTGCT TCCAACCAAG 
TGTTTAAGCA GCATGTCCAC AGCAATCAGC AAAATGACTC CTCCGAGAAC AGTGCATTTA 
ACTGAAAAGG AGGAAGAAGT TGTTGACTGG TGGAGCAAAT TTTATGCATC TGTTGGAGAA 
CCTGAAAAGT GTGGGCAATA TATCCAGAAG GAATATGCCA AACTAAAGGT ATATAATTGT 
GAACTAGAGA GTGTTCCAGA GTTTAAGGGT CTGACAGACT TTTCAGATAC CTTTAAGCTT 
TACAGAGGGA AGTCTGATGA GATTGATGAT CCTTCAGTGG TAGGAGAGTT CAAGGGCTCC 
TTTAGAATCT ATCCACTGCC TGATGATCCA AATGTGCCAG CTCCTCCCAG GCAGTTTCGG 
GAATTACCTG ACAGTGTCCC ACAGGAATGC ATTGTGAGGA TTTATATTGT TCGGGGCATA 
GATCTCCAGC CTCAGGATAA CAATGGCCTG TGTGACCCTT ATATAAAAAT ATCCCTGAGT 
AAAAAAGTCA TTGAAGATCG AGATAATTAC ATTCCAAATA CACTCAACCC AATTTTTGGA 
AGGATGTATG AGCTAAGCTG CTTCTTACCT CAAGAAAAAG ACCTGAAAAT TTCTGTCTAT 
GATTATGACA CACTTACTCG TGATGAAAAA GTAGGAGAAA CTATCATTGA TCTAGAAAAT 
CGCTTCCTTT CCCGATTTGG GTCGAATTGT GGCATAGCAG AGCAATATTG CATTTCTGGA 
GTCAATACTT GGAGAGATCA ACTAAAACCA ACCCAATTGT TATGCAATGT AGCTAAGTTT 
AAAGGATTGC CACTCCCTAT TCTTTCTGAA GATGGAAATA GGCTAAGATT TGGAGGTCGA 
GAGTACACCT TGGAAGAATT TGAGGCCAAC AGAAATCTGC ACCAGCACCT GGGACCTGCA 
GAGGAGCGTC TTGCTCTGTA TATCCTTCGG ACTCAGGGTC AAGTCCCTGA GCATGTGGAA 
ACAAGGACTT TGTACAGCAC CTTCCAACCA AATATTTCAC AGGGAAAACT TCAGATGTGG 
GTGGATGTTT TCCCCAAAAG TTTGGGGCCA CCAGGCCCTC CTTTCAATAT CACTCCCCGG 
AAGGCCAAGA GGTATGTCCT ACGTGTGATC ATTTGGAACA CTAAAGATGT CATTCTTGAT 
GAGAAAAGTA TCACAGGAGA AGAGATGACT GACATCTATG TGAAAGGCTG GATTCCTGGG 
AATGAAGAAA ACAAGCAGAA GACAGATGTC CATTACAGGT CATTGGATGG TGAAGGAAAC 
TTTAATTGGA GATTTGTTTT CCCTTTTGAA TACCTGCCTG CTGAACAACT CTGTATTGTT 
TCTAAAAAAG AACATTTCTG GAGTATTGAC CAGACTGAAT TTCGAGTTCC TCCCAGACTC 
ATCATTCAGA TATGGGACAA TGATAAGTTT TCTTTAGATG ACTACTTGGG TTTTCTGGAA 
CTTGATTTAC ACAACACAAT TATCCCTGCA AAGTCTTCAG AAAAGTGCAA ATTGGACATG 
ATTTCAGAGT ATAGGGCAGT GAATCCTCTA AAGTCCAAGA CAGCCTCACT TTTTGAACAG 
AAATCGATGA AAGGATGGTG GCCATGCTAT GCAGAAAAAG ATGGCGCACG TGTGATGACT 
GGTAAAGTGG AGATGACTTT GGAAGTCCTC AATGAGAAGG AGGTTGATGA AAGACCAGCT 
GGGAAAGGAA GGGATGAGCC CAACATGAAC CCCAAGCTAG AATTACCAAA TCGACCAGAA 
ACTTCATTCC TTTGGTTTAC CAACCCATGC AAGACAATGA AATTCATCGT GTGGCGTCGA 
TTTAAATGGG TCATCATTGG CTTACTCATC CTGCTGGTCT TGTTGCTGTT TTTGGCAGTA 
CTCCTATATT CTCTGCCGAA CTATATATCA ATGAAGATCG TGAGACCAAA TGCAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_016426157.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016281643.1 Monodelphis domestica myoferlin (MYOF), transcript variant X3, mRNA.

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CloneID	OMc38446
Clone ID Related Accession (Same CDS sequence)	XM_016426157.1
Accession Version	XM_016426157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	myoferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTTG GGGTGTTCAT TTTGATGATC TTGAAGGTCT CTTCCAGCTA TAAATACTAT 
CATCCTATTA TCTTTCAGCT GGGGGTATGC AGAGGGATTC ATGAAAGAGG GCCTAAAAGA 
AAGGAAGGAG CTTCAAAAGA TGAAGATGAG AAGAAGAAAA CGAAGAAAAT TGATAATGAA 
CTCAACCCTG TCTGGAATGA GATATTTGAA TTTGATTTGA AGGGCATACC ATTGGACTTT 
TCATCTACTC TTGGCATCAC TGTGAAAGAT TTCGAGACAA TTGGACAAAA TAAACTGATT 
GGCGTTGCAA CAATATCACT GAAGGACTTG ATAGGTGGCC AGAGTCGATC TCTTCCTTAT 
AAACTCATAT CTTTGGTAAA TGAAAAGGGT CAAGATACTG GGGCTACAAT CGATCTGGTG 
GTTGCCTATG ATCCACCAGC AGCCATGGCA ACAAATCCAA ATGAACTAAG TGGGATCACC 
ATAGAAGGCA CGGGAGAAGA AGAGGAAGAA GACACAGGAG ATGAAGATAC TGCATTCAAA 
GTCATTGCCC CAAGGGTTCC AGCAACAAAT ATTTCTGAAG CCCAGCTGGC CAGGAGGATT 
ACAAAAGGAA AGAATACCCG AAAGATCCTT TCCAATAAGC CACAAGACTT CCAGATTCGT 
GTTAGAGTGA TTGAAGGGCG CCAATTAAGT GGGAATAACA TCAAACCAGT GGTCAAAGTC 
CATGTCTGTG GTCAGACACA TCGAACAAGA ATAAAAAGAG GGAATAATCC ATTTTTTGAC 
GAATTATTTT TCTACAATGT CCACATGACC CCTCTTGAAC TGGTTGATGA GACAATAAGC 
ATTCGGGTAT ATGATTCCCA TTCTTTGAGA GCAGACTGTC TGATGGGAGA ATTTAAGATT 
GATATTGGTT ACATTTATGA TGAACCTGGT CATGCTGTCA TGAGAAAATG GCTTCTTCTA 
AATGACCCTG AAGATATAAG TTCTGGTGCT AAAGGTTACA TGAAAGTCAG CATGTTTGTC 
CTTGGAACAG GAGATGAACC ACCAACAGAG AAGAGAGATC GAGATAATGA CAGTGATGAT 
GTGGAGAGTA ATTTGTTACT TCCTGCTGGA ATTGCCATGC GGTGGGTCAC TTTCCTCCTG 
AAAATTTACA GAGCTGAGGA TATTCCTCAG ATGGATGATG CCTTTTCACA GACTGTCAAA 
GAAATATTTG GAGCCAATGT AGACAAGAAA AACTTAGTGG ATCCATTTGT AGAAGTTTGT 
TTTGCTGGGA AAAAGGTTTC CACAGGCATA ATAGAGAAAA ATGCAAATCC AGAGTGGAAT 
CAAGTTGTCA ATCTTCAAAT CAAGTTTCCT TCAATGTGTG AAAAAATAAA GTTAACAGTA 
TATGACTGGG ACCGCCTTAC CAAGAATGAT GTAGTAGGGA CAACCTATCT GTATCTTTCT 
AAAATTGCTG CATCGGGTGG GGAAGTTGAA GAGAAAACAG GAGAAACAGA AGTGGGCTTT 
GTACCAACAT TTGGACCTTG TTATCTAAAT CTTTATGGAA GTCCAAGAGA ATACACTGGA 
TTCCCAGATC CATATGATGA CTTGAATTCT GGAAAGGGTG AAGGAGTTGC CTACAGAGGT 
CGAATCTTGG TAGAACTGAA TACTTTACTT GAGAAAACAC CCCCTGACAA GAAGGTTGAA 
ACCATATCCA ATGATGATTT GCTGGTTGTT GAGAAATATC AGCGTAGGCG AAAATATAGC 
CTGTCTGCGG TATTTCATTC TGCAACCATG CTGCAAGATA TTGGAGAGGC CATTCAGTTT 
GAAGTCAGCA TTGGAAATTA CGGCAATAAG TTTGATGCTA CCTGTAAGCC TTTGGCATCG 
ACAACTCAAT ACAGTCGGGC TGTGTTTGAT GGAAATTTCT ATTATTATTT GCCATGGGCC 
CAGAAGAAGC CAGTGGTGAC TCTAACTTCT TATTGGGAAG ACATTAGTCA CAGACTGGAT 
GCAGTGAACA CCATTCTTGT TATGGCAGAA CGGCTGCACG TGAACATTGA GAATTTAAAA 
TCAGGAATAC AAGCCAAAAT GTCTGGGAAT CATTTGGCTG AAATGTGGCT GAAGTTGATT 
GATGAACTTA TCGAAGATAC AAGCATGCCA TTGCCTTCAC TAGAAGGGAA ATCTAATGTC 
ACCATTCTAG ACACTCAGAT CCAAAAGCTG CGTCTTCGAT CCCTTATCCA GATTCAAGAA 
GCAGCTAGAA AAATGAGGTC TGAAGCCAAA CATGTGAAGT CTACTTTGAC AGAAATTGAA 
GATTGGTTTG ATAAGCTAAT GCAACTTTCT GAAGAGCCAC AAAACAGTAT GCCCGATATT 
ATCATATGGA TGATCCGAGG AGAGAAAAGA TTGGCCTATG CTAGAATTCC TGCACACCAG 
GTTTTATATT CTACTACCAG TGAAGAAGCT TCTGGAAAGT ACTGTGGGAA GACCCAGACC 
ATCTTTTTGA AGTACCCACA GGACAGGAGC TATGGTGTGA AGGTTCCAGC AGAATTGCGG 
GTTAACATTT GGCTTGGATT AAGTGCTGTT GAAAAGAAGT TTAACAGCTT TGCTGAAGGA 
ACATTCACTG TCTTTGCAGA AATGTATGAA AATCAAGCTT TGATGTTTGG AAAATGGGGC 
ACTTCTGGAC TTGTGGGTCG ACATAAATTT TCAGATGTCA CAGGCAAGAT AAAGCTCAAG 
AAAGAGTTCT TTTTGCCCCC TAAAGGCTGG GAATGGGAAG GTGAATGGAT GGTGGATCCT 
GAAAGAAGTT TATTGACTGA AGCGGATGCT GGCCACACAC ACTTTACAGA TGAAGTGTAT 
CAGAATGAAA GCCGCTATCC AGGTGGAGAA TGGAAGGCAA TGGAGGACAG CTACACAGAT 
GTGAATGGAG AAAAAGCAGC ATCTCCTAAT GAGATGACTT GCCCCCCTGG CTGGGAATGG 
GAAGATGATG CTTGGATGTA TGATATAAAC CGAGCTGTAG ATGAAAAAGG CTGGGAATAT 
GGAATTACTA TTCCTCCTGA TAACAAGCCA AAGTCCTGGG TTGCTGCAGA GAAGATGTAT 
CATACACACA GGCGGCGGCG AATGGTCCGC AAACGCAAAA AGGATGTGAC ACAGTCGGCT 
GCAAGTACTG CAAGGGGGTG GGAAGCACTA GAAGATAAAG AAGGCTGGGA ATATGCATCT 
TTAATTGGAT GGAAATTTCA CTGGAAACAG CGTAGTTCAG ATACTTTTCG TCGAAGACGC 
TGGAGAAGAA AGATGGCTCC TTCTGAAACA CATGGTTCAG CTGCTATCTT TAAACTTGAA 
GGTGCCATTG GAGCAGACAC TACTGAAGAT GGAGATGAAA AGAGCACGGA GAAAAACAAA 
CATAGTGCAA CAACCGTGTT TGGTGCAAAC ACTCCAATTG TTTCCTGTAA TTTTGACAGA 
GTATATAATT ATCATCTTCG CTGCTATATA TATCAAGCTC GAAATCTTAT GGCTTTAGAT 
AAAGATAGTT TTTCAGATCC CTATGCTCAT GTTTCATTCC TCCATCGGAG CCAAACAACT 
GAAATCATCC ACTCAACACT GAATCCAACC TGGGACCAAA CTCTAATATT TGATGACATT 
GAAATTTTTG GAGAACCACA AACAATCATA CAAAACCCAC CCAAAGTTGT CATTGAACTC 
TTTGACAATG ACCAAGTGGG CAAGGATGAA TTTTTAGGTC GAAGTTTTTG CTCCCCAGTT 
GTCAAGCTAA ATCCAGAAGT TGACATCACC CCTAAGCTTC TCTGGCAACC AGTCATAAAT 
GGAGATAAGG CTTGTGGGGA TGTACTTGTA GCTGCTGAGC TTATTCTCAA AGATAAGGTT 
GGCTCCAATC TTCCAATTCT CCCATCTCAG AGGGCTCCAA ATCTCTACAT GGTTCCTCAA 
GGAATTAGGC CTGTGGTTCA ACTCACTGCT ATTGAGATTT TAGCTTGGGG TTTAAGAAAC 
ATGAAAAACT ACCAGATGGC TTCTGTGACT TCCCCAAGTC TTATCATAGA GTGTGGAGGA 
GAAAGGATAG AATCAGCATT GATCAAAAAC CTCAAGAAGA CACCCAACTT TCCTAGTTCT 
GTTCTCTTCA TGAAAGTGCT ATTGCCCAAG GAAGAATTGT ACACACCACC ATTGGTTATC 
AAAGTGATCG ACCACAGACA ATTTGGGCGA AAGCCCATCG TGGGTCAGTG CACCATCGAC 
CTTCTTGAAC AGTTTCGTTG TGACCCTTAT GCATTTAAGG AAGATATTGC ACCCCAGGTA 
AAAGCTTCTA TCTTGTCTGC CCCACTTAGC CGGGATGTGG TTATTGAAGT TGAGGACACA 
AAACCATTGC TTCCAACCAA GTTCTCGGTA TCTCCATCCT CTTGCCATTT GTGTGGCTCT 
CTGTCCTGCT GTTGCACACG TGTCCATGAT CAGTTAACTG AAAAGGAGGA AGAAGTTGTT 
GACTGGTGGA GCAAATTTTA TGCATCTGTT GGAGAACCTG AAAAGTGTGG GCAATATATC 
CAGAAGGAAT ATGCCAAACT AAAGGTATAT AATTGTGAAC TAGAGAGTGT TCCAGAGTTT 
AAGGGTCTGA CAGACTTTTC AGATACCTTT AAGCTTTACA GAGGGAAGTC TGATGAGATT 
GATGATCCTT CAGTGGTAGG AGAGTTCAAG GGCTCCTTTA GAATCTATCC ACTGCCTGAT 
GATCCAAATG TGCCAGCTCC TCCCAGGCAG TTTCGGGAAT TACCTGACAG TGTCCCACAG 
GAATGCATTG TGAGGATTTA TATTGTTCGG GGCATAGATC TCCAGCCTCA GGATAACAAT 
GGCCTGTGTG ACCCTTATAT AAAAATATCC CTGAGTAAAA AAGTCATTGA AGATCGAGAT 
AATTACATTC CAAATACACT CAACCCAATT TTTGGAAGGA TGTATGAGCT AAGCTGCTTC 
TTACCTCAAG AAAAAGACCT GAAAATTTCT GTCTATGATT ATGACACACT TACTCGTGAT 
GAAAAAGTAG GAGAAACTAT CATTGATCTA GAAAATCGCT TCCTTTCCCG ATTTGGGTCG 
AATTGTGGCA TAGCAGAGCA ATATTGCATT TCTGGAGTCA ATACTTGGAG AGATCAACTA 
AAACCAACCC AATTGTTATG CAATGTAGCT AAGTTTAAAG GATTGCCACT CCCTATTCTT 
TCTGAAGATG GAAATAGGCT AAGATTTGGA GGTCGAGAGT ACACCTTGGA AGAATTTGAG 
GCCAACAGAA ATCTGCACCA GCACCTGGGA CCTGCAGAGG AGCGTCTTGC TCTGTATATC 
CTTCGGACTC AGGGTCAAGT CCCTGAGCAT GTGGAAACAA GGACTTTGTA CAGCACCTTC 
CAACCAAATA TTTCACAGGG AAAACTTCAG ATGTGGGTGG ATGTTTTCCC CAAAAGTTTG 
GGGCCACCAG GCCCTCCTTT CAATATCACT CCCCGGAAGG CCAAGAGGTA TGTCCTACGT 
GTGATCATTT GGAACACTAA AGATGTCATT CTTGATGAGA AAAGTATCAC AGGAGAAGAG 
ATGACTGACA TCTATGTGAA AGGCTGGATT CCTGGGAATG AAGAAAACAA GCAGAAGACA 
GATGTCCATT ACAGGTCATT GGATGGTGAA GGAAACTTTA ATTGGAGATT TGTTTTCCCT 
TTTGAATACC TGCCTGCTGA ACAACTCTGT ATTGTTTCTA AAAAAGAACA TTTCTGGAGT 
ATTGACCAGA CTGAATTTCG AGTTCCTCCC AGACTCATCA TTCAGATATG GGACAATGAT 
AAGTTTTCTT TAGATGACTA CTTGGGTTTT CTGGAACTTG ATTTACACAA CACAATTATC 
CCTGCAAAGT CTTCAGAAAA GTGCAAATTG GACATGATTT CAGAGTATAG GGCAGTGAAT 
CCTCTAAAGT CCAAGACAGC CTCACTTTTT GAACAGAAAT CGATGAAAGG ATGGTGGCCA 
TGCTATGCAG AAAAAGATGG CGCACGTGTG ATGACTGGTA AAGTGGAGAT GACTTTGGAA 
GTCCTCAATG AGAAGGAGGT TGATGAAAGA CCAGCTGGGA AAGGAAGGGA TGAGCCCAAC 
ATGAACCCCA AGCTAGAATT ACCAAATCGA CCAGAAACTT CATTCCTTTG GTTTACCAAC 
CCATGCAAGA CAATGAAATT CATCGTGTGG CGTCGATTTA AATGGGTCAT CATTGGCTTA 
CTCATCCTGC TGGTCTTGTT GCTGTTTTTG GCAGTACTCC TATATTCTCT GCCGAACTAT 
ATATCAATGA AGATCGTGAG ACCAAATGCA AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016281643.1
CDS	16..6291
Translation	MGVGVFILMILKVSSSYKYYHPIIFQLGVCRGIHERGPKRKEGASKDEDEKKKTKKIDNELNPVWNEIFEFDLKGIPLDFSSTLGITVKDFETIGQNKLIGVATISLKDLIGGQSRSLPYKLISLVNEKGQDTGATIDLVVAYDPPAAMATNPNELSGITIEGTGEEEEEDTGDEDTAFKVIAPRVPATNISEAQLARRITKGKNTRKILSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHMTPLELVDETISIRVYDSHSLRADCLMGEFKIDIGYIYDEPGHAVMRKWLLLNDPEDISSGAKGYMKVSMFVLGTGDEPPTEKRDRDNDSDDVESNLLLPAGIAMRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGANVDKKNLVDPFVEVCFAGKKVSTGIIEKNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEEKTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDDLNSGKGEGVAYRGRILVELNTLLEKTPPDKKVETISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNFYYYLPWAQKKPVVTLTSYWEDISHRLDAVNTILVMAERLHVNIENLKSGIQAKMSGNHLAEMWLKLIDELIEDTSMPLPSLEGKSNVTILDTQIQKLRLRSLIQIQEAARKMRSEAKHVKSTLTEIEDWFDKLMQLSEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTTSEEASGKYCGKTQTIFLKYPQDRSYGVKVPAELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKKEFFLPPKGWEWEGEWMVDPERSLLTEADAGHTHFTDEVYQNESRYPGGEWKAMEDSYTDVNGEKAASPNEMTCPPGWEWEDDAWMYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRMVRKRKKDVTQSAASTARGWEALEDKEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGSAAIFKLEGAIGADTTEDGDEKSTEKNKHSATTVFGANTPIVSCNFDRVYNYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSQTTEIIHSTLNPTWDQTLIFDDIEIFGEPQTIIQNPPKVVIELFDNDQVGKDEFLGRSFCSPVVKLNPEVDITPKLLWQPVINGDKACGDVLVAAELILKDKVGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIIECGGERIESALIKNLKKTPNFPSSVLFMKVLLPKEELYTPPLVIKVIDHRQFGRKPIVGQCTIDLLEQFRCDPYAFKEDIAPQVKASILSAPLSRDVVIEVEDTKPLLPTKFSVSPSSCHLCGSLSCCCTRVHDQLTEKEEEVVDWWSKFYASVGEPEKCGQYIQKEYAKLKVYNCELESVPEFKGLTDFSDTFKLYRGKSDEIDDPSVVGEFKGSFRIYPLPDDPNVPAPPRQFRELPDSVPQECIVRIYIVRGIDLQPQDNNGLCDPYIKISLSKKVIEDRDNYIPNTLNPIFGRMYELSCFLPQEKDLKISVYDYDTLTRDEKVGETIIDLENRFLSRFGSNCGIAEQYCISGVNTWRDQLKPTQLLCNVAKFKGLPLPILSEDGNRLRFGGREYTLEEFEANRNLHQHLGPAEERLALYILRTQGQVPEHVETRTLYSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKRYVLRVIIWNTKDVILDEKSITGEEMTDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFEYLPAEQLCIVSKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHNTIIPAKSSEKCKLDMISEYRAVNPLKSKTASLFEQKSMKGWWPCYAEKDGARVMTGKVEMTLEVLNEKEVDERPAGKGRDEPNMNPKLELPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLILLVLLLFLAVLLYSLPNYISMKIVRPNAK

Target ORF information:

RefSeq Version	XM_016426157.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica myoferlin (MYOF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016426157.1

ORF Insert Sequence:

ATGGGGGTTG GGGTGTTCAT TTTGATGATC TTGAAGGTCT CTTCCAGCTA TAAATACTAT 
CATCCTATTA TCTTTCAGCT GGGGGTATGC AGAGGGATTC ATGAAAGAGG GCCTAAAAGA 
AAGGAAGGAG CTTCAAAAGA TGAAGATGAG AAGAAGAAAA CGAAGAAAAT TGATAATGAA 
CTCAACCCTG TCTGGAATGA GATATTTGAA TTTGATTTGA AGGGCATACC ATTGGACTTT 
TCATCTACTC TTGGCATCAC TGTGAAAGAT TTCGAGACAA TTGGACAAAA TAAACTGATT 
GGCGTTGCAA CAATATCACT GAAGGACTTG ATAGGTGGCC AGAGTCGATC TCTTCCTTAT 
AAACTCATAT CTTTGGTAAA TGAAAAGGGT CAAGATACTG GGGCTACAAT CGATCTGGTG 
GTTGCCTATG ATCCACCAGC AGCCATGGCA ACAAATCCAA ATGAACTAAG TGGGATCACC 
ATAGAAGGCA CGGGAGAAGA AGAGGAAGAA GACACAGGAG ATGAAGATAC TGCATTCAAA 
GTCATTGCCC CAAGGGTTCC AGCAACAAAT ATTTCTGAAG CCCAGCTGGC CAGGAGGATT 
ACAAAAGGAA AGAATACCCG AAAGATCCTT TCCAATAAGC CACAAGACTT CCAGATTCGT 
GTTAGAGTGA TTGAAGGGCG CCAATTAAGT GGGAATAACA TCAAACCAGT GGTCAAAGTC 
CATGTCTGTG GTCAGACACA TCGAACAAGA ATAAAAAGAG GGAATAATCC ATTTTTTGAC 
GAATTATTTT TCTACAATGT CCACATGACC CCTCTTGAAC TGGTTGATGA GACAATAAGC 
ATTCGGGTAT ATGATTCCCA TTCTTTGAGA GCAGACTGTC TGATGGGAGA ATTTAAGATT 
GATATTGGTT ACATTTATGA TGAACCTGGT CATGCTGTCA TGAGAAAATG GCTTCTTCTA 
AATGACCCTG AAGATATAAG TTCTGGTGCT AAAGGTTACA TGAAAGTCAG CATGTTTGTC 
CTTGGAACAG GAGATGAACC ACCAACAGAG AAGAGAGATC GAGATAATGA CAGTGATGAT 
GTGGAGAGTA ATTTGTTACT TCCTGCTGGA ATTGCCATGC GGTGGGTCAC TTTCCTCCTG 
AAAATTTACA GAGCTGAGGA TATTCCTCAG ATGGATGATG CCTTTTCACA GACTGTCAAA 
GAAATATTTG GAGCCAATGT AGACAAGAAA AACTTAGTGG ATCCATTTGT AGAAGTTTGT 
TTTGCTGGGA AAAAGGTTTC CACAGGCATA ATAGAGAAAA ATGCAAATCC AGAGTGGAAT 
CAAGTTGTCA ATCTTCAAAT CAAGTTTCCT TCAATGTGTG AAAAAATAAA GTTAACAGTA 
TATGACTGGG ACCGCCTTAC CAAGAATGAT GTAGTAGGGA CAACCTATCT GTATCTTTCT 
AAAATTGCTG CATCGGGTGG GGAAGTTGAA GAGAAAACAG GAGAAACAGA AGTGGGCTTT 
GTACCAACAT TTGGACCTTG TTATCTAAAT CTTTATGGAA GTCCAAGAGA ATACACTGGA 
TTCCCAGATC CATATGATGA CTTGAATTCT GGAAAGGGTG AAGGAGTTGC CTACAGAGGT 
CGAATCTTGG TAGAACTGAA TACTTTACTT GAGAAAACAC CCCCTGACAA GAAGGTTGAA 
ACCATATCCA ATGATGATTT GCTGGTTGTT GAGAAATATC AGCGTAGGCG AAAATATAGC 
CTGTCTGCGG TATTTCATTC TGCAACCATG CTGCAAGATA TTGGAGAGGC CATTCAGTTT 
GAAGTCAGCA TTGGAAATTA CGGCAATAAG TTTGATGCTA CCTGTAAGCC TTTGGCATCG 
ACAACTCAAT ACAGTCGGGC TGTGTTTGAT GGAAATTTCT ATTATTATTT GCCATGGGCC 
CAGAAGAAGC CAGTGGTGAC TCTAACTTCT TATTGGGAAG ACATTAGTCA CAGACTGGAT 
GCAGTGAACA CCATTCTTGT TATGGCAGAA CGGCTGCACG TGAACATTGA GAATTTAAAA 
TCAGGAATAC AAGCCAAAAT GTCTGGGAAT CATTTGGCTG AAATGTGGCT GAAGTTGATT 
GATGAACTTA TCGAAGATAC AAGCATGCCA TTGCCTTCAC TAGAAGGGAA ATCTAATGTC 
ACCATTCTAG ACACTCAGAT CCAAAAGCTG CGTCTTCGAT CCCTTATCCA GATTCAAGAA 
GCAGCTAGAA AAATGAGGTC TGAAGCCAAA CATGTGAAGT CTACTTTGAC AGAAATTGAA 
GATTGGTTTG ATAAGCTAAT GCAACTTTCT GAAGAGCCAC AAAACAGTAT GCCCGATATT 
ATCATATGGA TGATCCGAGG AGAGAAAAGA TTGGCCTATG CTAGAATTCC TGCACACCAG 
GTTTTATATT CTACTACCAG TGAAGAAGCT TCTGGAAAGT ACTGTGGGAA GACCCAGACC 
ATCTTTTTGA AGTACCCACA GGACAGGAGC TATGGTGTGA AGGTTCCAGC AGAATTGCGG 
GTTAACATTT GGCTTGGATT AAGTGCTGTT GAAAAGAAGT TTAACAGCTT TGCTGAAGGA 
ACATTCACTG TCTTTGCAGA AATGTATGAA AATCAAGCTT TGATGTTTGG AAAATGGGGC 
ACTTCTGGAC TTGTGGGTCG ACATAAATTT TCAGATGTCA CAGGCAAGAT AAAGCTCAAG 
AAAGAGTTCT TTTTGCCCCC TAAAGGCTGG GAATGGGAAG GTGAATGGAT GGTGGATCCT 
GAAAGAAGTT TATTGACTGA AGCGGATGCT GGCCACACAC ACTTTACAGA TGAAGTGTAT 
CAGAATGAAA GCCGCTATCC AGGTGGAGAA TGGAAGGCAA TGGAGGACAG CTACACAGAT 
GTGAATGGAG AAAAAGCAGC ATCTCCTAAT GAGATGACTT GCCCCCCTGG CTGGGAATGG 
GAAGATGATG CTTGGATGTA TGATATAAAC CGAGCTGTAG ATGAAAAAGG CTGGGAATAT 
GGAATTACTA TTCCTCCTGA TAACAAGCCA AAGTCCTGGG TTGCTGCAGA GAAGATGTAT 
CATACACACA GGCGGCGGCG AATGGTCCGC AAACGCAAAA AGGATGTGAC ACAGTCGGCT 
GCAAGTACTG CAAGGGGGTG GGAAGCACTA GAAGATAAAG AAGGCTGGGA ATATGCATCT 
TTAATTGGAT GGAAATTTCA CTGGAAACAG CGTAGTTCAG ATACTTTTCG TCGAAGACGC 
TGGAGAAGAA AGATGGCTCC TTCTGAAACA CATGGTTCAG CTGCTATCTT TAAACTTGAA 
GGTGCCATTG GAGCAGACAC TACTGAAGAT GGAGATGAAA AGAGCACGGA GAAAAACAAA 
CATAGTGCAA CAACCGTGTT TGGTGCAAAC ACTCCAATTG TTTCCTGTAA TTTTGACAGA 
GTATATAATT ATCATCTTCG CTGCTATATA TATCAAGCTC GAAATCTTAT GGCTTTAGAT 
AAAGATAGTT TTTCAGATCC CTATGCTCAT GTTTCATTCC TCCATCGGAG CCAAACAACT 
GAAATCATCC ACTCAACACT GAATCCAACC TGGGACCAAA CTCTAATATT TGATGACATT 
GAAATTTTTG GAGAACCACA AACAATCATA CAAAACCCAC CCAAAGTTGT CATTGAACTC 
TTTGACAATG ACCAAGTGGG CAAGGATGAA TTTTTAGGTC GAAGTTTTTG CTCCCCAGTT 
GTCAAGCTAA ATCCAGAAGT TGACATCACC CCTAAGCTTC TCTGGCAACC AGTCATAAAT 
GGAGATAAGG CTTGTGGGGA TGTACTTGTA GCTGCTGAGC TTATTCTCAA AGATAAGGTT 
GGCTCCAATC TTCCAATTCT CCCATCTCAG AGGGCTCCAA ATCTCTACAT GGTTCCTCAA 
GGAATTAGGC CTGTGGTTCA ACTCACTGCT ATTGAGATTT TAGCTTGGGG TTTAAGAAAC 
ATGAAAAACT ACCAGATGGC TTCTGTGACT TCCCCAAGTC TTATCATAGA GTGTGGAGGA 
GAAAGGATAG AATCAGCATT GATCAAAAAC CTCAAGAAGA CACCCAACTT TCCTAGTTCT 
GTTCTCTTCA TGAAAGTGCT ATTGCCCAAG GAAGAATTGT ACACACCACC ATTGGTTATC 
AAAGTGATCG ACCACAGACA ATTTGGGCGA AAGCCCATCG TGGGTCAGTG CACCATCGAC 
CTTCTTGAAC AGTTTCGTTG TGACCCTTAT GCATTTAAGG AAGATATTGC ACCCCAGGTA 
AAAGCTTCTA TCTTGTCTGC CCCACTTAGC CGGGATGTGG TTATTGAAGT TGAGGACACA 
AAACCATTGC TTCCAACCAA GTTCTCGGTA TCTCCATCCT CTTGCCATTT GTGTGGCTCT 
CTGTCCTGCT GTTGCACACG TGTCCATGAT CAGTTAACTG AAAAGGAGGA AGAAGTTGTT 
GACTGGTGGA GCAAATTTTA TGCATCTGTT GGAGAACCTG AAAAGTGTGG GCAATATATC 
CAGAAGGAAT ATGCCAAACT AAAGGTATAT AATTGTGAAC TAGAGAGTGT TCCAGAGTTT 
AAGGGTCTGA CAGACTTTTC AGATACCTTT AAGCTTTACA GAGGGAAGTC TGATGAGATT 
GATGATCCTT CAGTGGTAGG AGAGTTCAAG GGCTCCTTTA GAATCTATCC ACTGCCTGAT 
GATCCAAATG TGCCAGCTCC TCCCAGGCAG TTTCGGGAAT TACCTGACAG TGTCCCACAG 
GAATGCATTG TGAGGATTTA TATTGTTCGG GGCATAGATC TCCAGCCTCA GGATAACAAT 
GGCCTGTGTG ACCCTTATAT AAAAATATCC CTGAGTAAAA AAGTCATTGA AGATCGAGAT 
AATTACATTC CAAATACACT CAACCCAATT TTTGGAAGGA TGTATGAGCT AAGCTGCTTC 
TTACCTCAAG AAAAAGACCT GAAAATTTCT GTCTATGATT ATGACACACT TACTCGTGAT 
GAAAAAGTAG GAGAAACTAT CATTGATCTA GAAAATCGCT TCCTTTCCCG ATTTGGGTCG 
AATTGTGGCA TAGCAGAGCA ATATTGCATT TCTGGAGTCA ATACTTGGAG AGATCAACTA 
AAACCAACCC AATTGTTATG CAATGTAGCT AAGTTTAAAG GATTGCCACT CCCTATTCTT 
TCTGAAGATG GAAATAGGCT AAGATTTGGA GGTCGAGAGT ACACCTTGGA AGAATTTGAG 
GCCAACAGAA ATCTGCACCA GCACCTGGGA CCTGCAGAGG AGCGTCTTGC TCTGTATATC 
CTTCGGACTC AGGGTCAAGT CCCTGAGCAT GTGGAAACAA GGACTTTGTA CAGCACCTTC 
CAACCAAATA TTTCACAGGG AAAACTTCAG ATGTGGGTGG ATGTTTTCCC CAAAAGTTTG 
GGGCCACCAG GCCCTCCTTT CAATATCACT CCCCGGAAGG CCAAGAGGTA TGTCCTACGT 
GTGATCATTT GGAACACTAA AGATGTCATT CTTGATGAGA AAAGTATCAC AGGAGAAGAG 
ATGACTGACA TCTATGTGAA AGGCTGGATT CCTGGGAATG AAGAAAACAA GCAGAAGACA 
GATGTCCATT ACAGGTCATT GGATGGTGAA GGAAACTTTA ATTGGAGATT TGTTTTCCCT 
TTTGAATACC TGCCTGCTGA ACAACTCTGT ATTGTTTCTA AAAAAGAACA TTTCTGGAGT 
ATTGACCAGA CTGAATTTCG AGTTCCTCCC AGACTCATCA TTCAGATATG GGACAATGAT 
AAGTTTTCTT TAGATGACTA CTTGGGTTTT CTGGAACTTG ATTTACACAA CACAATTATC 
CCTGCAAAGT CTTCAGAAAA GTGCAAATTG GACATGATTT CAGAGTATAG GGCAGTGAAT 
CCTCTAAAGT CCAAGACAGC CTCACTTTTT GAACAGAAAT CGATGAAAGG ATGGTGGCCA 
TGCTATGCAG AAAAAGATGG CGCACGTGTG ATGACTGGTA AAGTGGAGAT GACTTTGGAA 
GTCCTCAATG AGAAGGAGGT TGATGAAAGA CCAGCTGGGA AAGGAAGGGA TGAGCCCAAC 
ATGAACCCCA AGCTAGAATT ACCAAATCGA CCAGAAACTT CATTCCTTTG GTTTACCAAC 
CCATGCAAGA CAATGAAATT CATCGTGTGG CGTCGATTTA AATGGGTCAT CATTGGCTTA 
CTCATCCTGC TGGTCTTGTT GCTGTTTTTG GCAGTACTCC TATATTCTCT GCCGAACTAT 
ATATCAATGA AGATCGTGAG ACCAAATGCA AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_007478795.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007478857.2 Monodelphis domestica myoferlin (MYOF), transcript variant X4, mRNA.

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CloneID	OMc38447
Clone ID Related Accession (Same CDS sequence)	XM_007478795.2
Accession Version	XM_007478795.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	myoferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007478795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGTTG GGGTGTTCAT TTTGATGATC TTGAAGGTCT CTTCCAGCTA TAAATACTAT 
CATCCTATTA TCTTTCAGCT GGGGGTATGC AGAGGGATTC ATGAAAGAGG GCCTAAAAGA 
AAGGAAGGAG CTTCAAAAGA TGAAGATGAG AAGAAGAAAA CGAAGAAAAT TGATAATGAA 
CTCAACCCTG TCTGGAATGA GATATTTGAA TTTGATTTGA AGGGCATACC ATTGGACTTT 
TCATCTACTC TTGGCATCAC TGTGAAAGAT TTCGAGACAA TTGGACAAAA TAAACTGATT 
GGCGTTGCAA CAATATCACT GAAGGACTTG ATAGGTGGCC AGAGTCGATC TCTTCCTTAT 
AAACTCATAT CTTTGGTAAA TGAAAAGGGT CAAGATACTG GGGCTACAAT CGATCTGGTG 
GTTGCCTATG ATCCACCAGC AGCCATGGCA ACAAATCCAA ATGAACTAAG TGGGATCACC 
ATAGAAGGCA CGGGAGAAGA AGAGGAAGAA GACACAGGAG ATGAAGATAC TGCATTCAAA 
GTCATTGCCC CAAGGGTTCC AGCAACAAAT ATTTCTGAAG CCCAGCTGGC CAGGAGGATT 
ACAAAAGGAA AGAATACCCG AAAGATCCTT TCCAATAAGC CACAAGACTT CCAGATTCGT 
GTTAGAGTGA TTGAAGGGCG CCAATTAAGT GGGAATAACA TCAAACCAGT GGTCAAAGTC 
CATGTCTGTG GTCAGACACA TCGAACAAGA ATAAAAAGAG GGAATAATCC ATTTTTTGAC 
GAATTATTTT TCTACAATGT CCACATGACC CCTCTTGAAC TGGTTGATGA GACAATAAGC 
ATTCGGGTAT ATGATTCCCA TTCTTTGAGA GCAGACTGTC TGATGGGAGA ATTTAAGATT 
GATATTGGTT ACATTTATGA TGAACCTGGT CATGCTGTCA TGAGAAAATG GCTTCTTCTA 
AATGACCCTG AAGATATAAG TTCTGGTGCT AAAGGTTACA TGAAAGTCAG CATGTTTGTC 
CTTGGAACAG GAGATGAACC ACCAACAGAG AAGAGAGATC GAGATAATGA CAGTGATGAT 
GTGGAGAGTA ATTTGTTACT TCCTGCTGGA ATTGCCATGC GGTGGGTCAC TTTCCTCCTG 
AAAATTTACA GAGCTGAGGA TATTCCTCAG ATGGATGATG CCTTTTCACA GACTGTCAAA 
GAAATATTTG GAGCCAATGT AGACAAGAAA AACTTAGTGG ATCCATTTGT AGAAGTTTGT 
TTTGCTGGGA AAAAGGTTTC CACAGGCATA ATAGAGAAAA ATGCAAATCC AGAGTGGAAT 
CAAGTTGTCA ATCTTCAAAT CAAGTTTCCT TCAATGTGTG AAAAAATAAA GTTAACAGTA 
TATGACTGGG ACCGCCTTAC CAAGAATGAT GTAGTAGGGA CAACCTATCT GTATCTTTCT 
AAAATTGCTG CATCGGGTGG GGAAGTTGAA GATTCCTCAC CTTCGGGAGC TTGGGCTGCA 
TCATCCACAG AGAAAACAGG AGAAACAGAA GTGGGCTTTG TACCAACATT TGGACCTTGT 
TATCTAAATC TTTATGGAAG TCCAAGAGAA TACACTGGAT TCCCAGATCC ATATGATGAC 
TTGAATTCTG GAAAGGGTGA AGGAGTTGCC TACAGAGGTC GAATCTTGGT AGAACTGAAT 
ACTTTACTTG AGAAAACACC CCCTGACAAG AAGGTTGAAA CCATATCCAA TGATGATTTG 
CTGGTTGTTG AGAAATATCA GCGTAGGCGA AAATATAGCC TGTCTGCGGT ATTTCATTCT 
GCAACCATGC TGCAAGATAT TGGAGAGGCC ATTCAGTTTG AAGTCAGCAT TGGAAATTAC 
GGCAATAAGT TTGATGCTAC CTGTAAGCCT TTGGCATCGA CAACTCAATA CAGTCGGGCT 
GTGTTTGATG GAAATTTCTA TTATTATTTG CCATGGGCCC AGAAGAAGCC AGTGGTGACT 
CTAACTTCTT ATTGGGAAGA CATTAGTCAC AGACTGGATG CAGTGAACAC CATTCTTGTT 
ATGGCAGAAC GGCTGCACGT GAACATTGAG AATTTAAAAT CAGGAATACA AGCCAAAATG 
TCTGGGAATC ATTTGGCTGA AATGTGGCTG AAGTTGATTG ATGAACTTAT CGAAGATACA 
AGCATGCCAT TGCCTTCACT AGAAGGGAAA TCTAATGTCA CCATTCTAGA CACTCAGATC 
CAAAAGCTGC GTCTTCGATC CCTTATCCAG ATTCAAGAAG CAGCTAGAAA AATGAGGTCT 
GAAGCCAAAC ATGTGAAGTC TACTTTGACA GAAATTGAAG ATTGGTTTGA TAAGCTAATG 
CAACTTTCTG AAGAGCCACA AAACAGTATG CCCGATATTA TCATATGGAT GATCCGAGGA 
GAGAAAAGAT TGGCCTATGC TAGAATTCCT GCACACCAGG TTTTATATTC TACTACCAGT 
GAAGAAGCTT CTGGAAAGTA CTGTGGGAAG ACCCAGACCA TCTTTTTGAA GTACCCACAG 
GACAGGAGCT ATGGTGTGAA GGTTCCAGCA GAATTGCGGG TTAACATTTG GCTTGGATTA 
AGTGCTGTTG AAAAGAAGTT TAACAGCTTT GCTGAAGGAA CATTCACTGT CTTTGCAGAA 
ATGTATGAAA ATCAAGCTTT GATGTTTGGA AAATGGGGCA CTTCTGGACT TGTGGGTCGA 
CATAAATTTT CAGATGTCAC AGGCAAGATA AAGCTCAAGA AAGAGTTCTT TTTGCCCCCT 
AAAGGCTGGG AATGGGAAGG TGAATGGATG GTGGATCCTG AAAGAAGTTT ATTGACTGAA 
GCGGATGCTG GCCACACACA CTTTACAGAT GAAGTGTATC AGAATGAAAG CCGCTATCCA 
GGTGGAGAAT GGAAGGCAAT GGAGGACAGC TACACAGATG TGAATGGAGA AAAAGCAGCA 
TCTCCTAATG AGATGACTTG CCCCCCTGGC TGGGAATGGG AAGATGATGC TTGGATGTAT 
GATATAAACC GAGCTGTAGA TGAAAAAGGC TGGGAATATG GAATTACTAT TCCTCCTGAT 
AACAAGCCAA AGTCCTGGGT TGCTGCAGAG AAGATGTATC ATACACACAG GCGGCGGCGA 
ATGGTCCGCA AACGCAAAAA GGATGTGACA CAGTCGGCTG CAAGTACTGC AAGGGGGTGG 
GAAGCACTAG AAGATAAAGA AGGCTGGGAA TATGCATCTT TAATTGGATG GAAATTTCAC 
TGGAAACAGC GTAGTTCAGA TACTTTTCGT CGAAGACGCT GGAGAAGAAA GATGGCTCCT 
TCTGAAACAC ATGGTTCAGC TGCTATCTTT AAACTTGAAG GTGCCATTGG AGCAGACACT 
ACTGAAGATG GAGATGAAAA GAGCACGGAG AAAAACAAAC ATAGTGCAAC AACCGTGTTT 
GGTGCAAACA CTCCAATTGT TTCCTGTAAT TTTGACAGAG TATATAATTA TCATCTTCGC 
TGCTATATAT ATCAAGCTCG AAATCTTATG GCTTTAGATA AAGATAGTTT TTCAGATCCC 
TATGCTCATG TTTCATTCCT CCATCGGAGC CAAACAACTG AAATCATCCA CTCAACACTG 
AATCCAACCT GGGACCAAAC TCTAATATTT GATGACATTG AAATTTTTGG AGAACCACAA 
ACAATCATAC AAAACCCACC CAAAGTTGTC ATTGAACTCT TTGACAATGA CCAAGTGGGC 
AAGGATGAAT TTTTAGGTCG AAGTTTTTGC TCCCCAGTTG TCAAGCTAAA TCCAGAAGTT 
GACATCACCC CTAAGCTTCT CTGGCAACCA GTCATAAATG GAGATAAGGC TTGTGGGGAT 
GTACTTGTAG CTGCTGAGCT TATTCTCAAA GATAAGGTTG GCTCCAATCT TCCAATTCTC 
CCATCTCAGA GGGCTCCAAA TCTCTACATG GTTCCTCAAG GAATTAGGCC TGTGGTTCAA 
CTCACTGCTA TTGAGATTTT AGCTTGGGGT TTAAGAAACA TGAAAAACTA CCAGATGGCT 
TCTGTGACTT CCCCAAGTCT TATCATAGAG TGTGGAGGAG AAAGGATAGA ATCAGCATTG 
ATCAAAAACC TCAAGAAGAC ACCCAACTTT CCTAGTTCTG TTCTCTTCAT GAAAGTGCTA 
TTGCCCAAGG AAGAATTGTA CACACCACCA TTGGTTATCA AAGTGATCGA CCACAGACAA 
TTTGGGCGAA AGCCCATCGT GGGTCAGTGC ACCATCGACC TTCTTGAACA GTTTCGTTGT 
GACCCTTATG CATTTAAGGA AGATATTGCA CCCCAGGTAA AAGCTTCTAT CTTGTCTGCC 
CCACTTAGCC GGGATGTGGT TATTGAAGTT GAGGACACAA AACCATTGCT TCCAACCAAG 
TTAACTGAAA AGGAGGAAGA AGTTGTTGAC TGGTGGAGCA AATTTTATGC ATCTGTTGGA 
GAACCTGAAA AGTGTGGGCA ATATATCCAG AAGGAATATG CCAAACTAAA GGTATATAAT 
TGTGAACTAG AGAGTGTTCC AGAGTTTAAG GGTCTGACAG ACTTTTCAGA TACCTTTAAG 
CTTTACAGAG GGAAGTCTGA TGAGATTGAT GATCCTTCAG TGGTAGGAGA GTTCAAGGGC 
TCCTTTAGAA TCTATCCACT GCCTGATGAT CCAAATGTGC CAGCTCCTCC CAGGCAGTTT 
CGGGAATTAC CTGACAGTGT CCCACAGGAA TGCATTGTGA GGATTTATAT TGTTCGGGGC 
ATAGATCTCC AGCCTCAGGA TAACAATGGC CTGTGTGACC CTTATATAAA AATATCCCTG 
AGTAAAAAAG TCATTGAAGA TCGAGATAAT TACATTCCAA ATACACTCAA CCCAATTTTT 
GGAAGGATGT ATGAGCTAAG CTGCTTCTTA CCTCAAGAAA AAGACCTGAA AATTTCTGTC 
TATGATTATG ACACACTTAC TCGTGATGAA AAAGTAGGAG AAACTATCAT TGATCTAGAA 
AATCGCTTCC TTTCCCGATT TGGGTCGAAT TGTGGCATAG CAGAGCAATA TTGCATTTCT 
GGAGTCAATA CTTGGAGAGA TCAACTAAAA CCAACCCAAT TGTTATGCAA TGTAGCTAAG 
TTTAAAGGAT TGCCACTCCC TATTCTTTCT GAAGATGGAA ATAGGCTAAG ATTTGGAGGT 
CGAGAGTACA CCTTGGAAGA ATTTGAGGCC AACAGAAATC TGCACCAGCA CCTGGGACCT 
GCAGAGGAGC GTCTTGCTCT GTATATCCTT CGGACTCAGG GTCAAGTCCC TGAGCATGTG 
GAAACAAGGA CTTTGTACAG CACCTTCCAA CCAAATATTT CACAGGGAAA ACTTCAGATG 
TGGGTGGATG TTTTCCCCAA AAGTTTGGGG CCACCAGGCC CTCCTTTCAA TATCACTCCC 
CGGAAGGCCA AGAGGTATGT CCTACGTGTG ATCATTTGGA ACACTAAAGA TGTCATTCTT 
GATGAGAAAA GTATCACAGG AGAAGAGATG ACTGACATCT ATGTGAAAGG CTGGATTCCT 
GGGAATGAAG AAAACAAGCA GAAGACAGAT GTCCATTACA GGTCATTGGA TGGTGAAGGA 
AACTTTAATT GGAGATTTGT TTTCCCTTTT GAATACCTGC CTGCTGAACA ACTCTGTATT 
GTTTCTAAAA AAGAACATTT CTGGAGTATT GACCAGACTG AATTTCGAGT TCCTCCCAGA 
CTCATCATTC AGATATGGGA CAATGATAAG TTTTCTTTAG ATGACTACTT GGGTTTTCTG 
GAACTTGATT TACACAACAC AATTATCCCT GCAAAGTCTT CAGAAAAGTG CAAATTGGAC 
ATGATTTCAG AGTATAGGGC AGTGAATCCT CTAAAGTCCA AGACAGCCTC ACTTTTTGAA 
CAGAAATCGA TGAAAGGATG GTGGCCATGC TATGCAGAAA AAGATGGCGC ACGTGTGATG 
ACTGGTAAAG TGGAGATGAC TTTGGAAGTC CTCAATGAGA AGGAGGTTGA TGAAAGACCA 
GCTGGGAAAG GAAGGGATGA GCCCAACATG AACCCCAAGC TAGAATTACC AAATCGACCA 
GAAACTTCAT TCCTTTGGTT TACCAACCCA TGCAAGACAA TGAAATTCAT CGTGTGGCGT 
CGATTTAAAT GGGTCATCAT TGGCTTACTC ATCCTGCTGG TCTTGTTGCT GTTTTTGGCA 
GTACTCCTAT ATTCTCTGCC GAACTATATA TCAATGAAGA TCGTGAGACC AAATGCAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007478857.2
CDS	16..6258
Translation	MGVGVFILMILKVSSSYKYYHPIIFQLGVCRGIHERGPKRKEGASKDEDEKKKTKKIDNELNPVWNEIFEFDLKGIPLDFSSTLGITVKDFETIGQNKLIGVATISLKDLIGGQSRSLPYKLISLVNEKGQDTGATIDLVVAYDPPAAMATNPNELSGITIEGTGEEEEEDTGDEDTAFKVIAPRVPATNISEAQLARRITKGKNTRKILSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHMTPLELVDETISIRVYDSHSLRADCLMGEFKIDIGYIYDEPGHAVMRKWLLLNDPEDISSGAKGYMKVSMFVLGTGDEPPTEKRDRDNDSDDVESNLLLPAGIAMRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGANVDKKNLVDPFVEVCFAGKKVSTGIIEKNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEDSSPSGAWAASSTEKTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDDLNSGKGEGVAYRGRILVELNTLLEKTPPDKKVETISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNFYYYLPWAQKKPVVTLTSYWEDISHRLDAVNTILVMAERLHVNIENLKSGIQAKMSGNHLAEMWLKLIDELIEDTSMPLPSLEGKSNVTILDTQIQKLRLRSLIQIQEAARKMRSEAKHVKSTLTEIEDWFDKLMQLSEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTTSEEASGKYCGKTQTIFLKYPQDRSYGVKVPAELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKKEFFLPPKGWEWEGEWMVDPERSLLTEADAGHTHFTDEVYQNESRYPGGEWKAMEDSYTDVNGEKAASPNEMTCPPGWEWEDDAWMYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRMVRKRKKDVTQSAASTARGWEALEDKEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGSAAIFKLEGAIGADTTEDGDEKSTEKNKHSATTVFGANTPIVSCNFDRVYNYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSQTTEIIHSTLNPTWDQTLIFDDIEIFGEPQTIIQNPPKVVIELFDNDQVGKDEFLGRSFCSPVVKLNPEVDITPKLLWQPVINGDKACGDVLVAAELILKDKVGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIIECGGERIESALIKNLKKTPNFPSSVLFMKVLLPKEELYTPPLVIKVIDHRQFGRKPIVGQCTIDLLEQFRCDPYAFKEDIAPQVKASILSAPLSRDVVIEVEDTKPLLPTKLTEKEEEVVDWWSKFYASVGEPEKCGQYIQKEYAKLKVYNCELESVPEFKGLTDFSDTFKLYRGKSDEIDDPSVVGEFKGSFRIYPLPDDPNVPAPPRQFRELPDSVPQECIVRIYIVRGIDLQPQDNNGLCDPYIKISLSKKVIEDRDNYIPNTLNPIFGRMYELSCFLPQEKDLKISVYDYDTLTRDEKVGETIIDLENRFLSRFGSNCGIAEQYCISGVNTWRDQLKPTQLLCNVAKFKGLPLPILSEDGNRLRFGGREYTLEEFEANRNLHQHLGPAEERLALYILRTQGQVPEHVETRTLYSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKRYVLRVIIWNTKDVILDEKSITGEEMTDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFEYLPAEQLCIVSKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHNTIIPAKSSEKCKLDMISEYRAVNPLKSKTASLFEQKSMKGWWPCYAEKDGARVMTGKVEMTLEVLNEKEVDERPAGKGRDEPNMNPKLELPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLILLVLLLFLAVLLYSLPNYISMKIVRPNAK

Target ORF information:

RefSeq Version	XM_007478795.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica myoferlin (MYOF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007478795.2

ORF Insert Sequence:

ATGGGGGTTG GGGTGTTCAT TTTGATGATC TTGAAGGTCT CTTCCAGCTA TAAATACTAT 
CATCCTATTA TCTTTCAGCT GGGGGTATGC AGAGGGATTC ATGAAAGAGG GCCTAAAAGA 
AAGGAAGGAG CTTCAAAAGA TGAAGATGAG AAGAAGAAAA CGAAGAAAAT TGATAATGAA 
CTCAACCCTG TCTGGAATGA GATATTTGAA TTTGATTTGA AGGGCATACC ATTGGACTTT 
TCATCTACTC TTGGCATCAC TGTGAAAGAT TTCGAGACAA TTGGACAAAA TAAACTGATT 
GGCGTTGCAA CAATATCACT GAAGGACTTG ATAGGTGGCC AGAGTCGATC TCTTCCTTAT 
AAACTCATAT CTTTGGTAAA TGAAAAGGGT CAAGATACTG GGGCTACAAT CGATCTGGTG 
GTTGCCTATG ATCCACCAGC AGCCATGGCA ACAAATCCAA ATGAACTAAG TGGGATCACC 
ATAGAAGGCA CGGGAGAAGA AGAGGAAGAA GACACAGGAG ATGAAGATAC TGCATTCAAA 
GTCATTGCCC CAAGGGTTCC AGCAACAAAT ATTTCTGAAG CCCAGCTGGC CAGGAGGATT 
ACAAAAGGAA AGAATACCCG AAAGATCCTT TCCAATAAGC CACAAGACTT CCAGATTCGT 
GTTAGAGTGA TTGAAGGGCG CCAATTAAGT GGGAATAACA TCAAACCAGT GGTCAAAGTC 
CATGTCTGTG GTCAGACACA TCGAACAAGA ATAAAAAGAG GGAATAATCC ATTTTTTGAC 
GAATTATTTT TCTACAATGT CCACATGACC CCTCTTGAAC TGGTTGATGA GACAATAAGC 
ATTCGGGTAT ATGATTCCCA TTCTTTGAGA GCAGACTGTC TGATGGGAGA ATTTAAGATT 
GATATTGGTT ACATTTATGA TGAACCTGGT CATGCTGTCA TGAGAAAATG GCTTCTTCTA 
AATGACCCTG AAGATATAAG TTCTGGTGCT AAAGGTTACA TGAAAGTCAG CATGTTTGTC 
CTTGGAACAG GAGATGAACC ACCAACAGAG AAGAGAGATC GAGATAATGA CAGTGATGAT 
GTGGAGAGTA ATTTGTTACT TCCTGCTGGA ATTGCCATGC GGTGGGTCAC TTTCCTCCTG 
AAAATTTACA GAGCTGAGGA TATTCCTCAG ATGGATGATG CCTTTTCACA GACTGTCAAA 
GAAATATTTG GAGCCAATGT AGACAAGAAA AACTTAGTGG ATCCATTTGT AGAAGTTTGT 
TTTGCTGGGA AAAAGGTTTC CACAGGCATA ATAGAGAAAA ATGCAAATCC AGAGTGGAAT 
CAAGTTGTCA ATCTTCAAAT CAAGTTTCCT TCAATGTGTG AAAAAATAAA GTTAACAGTA 
TATGACTGGG ACCGCCTTAC CAAGAATGAT GTAGTAGGGA CAACCTATCT GTATCTTTCT 
AAAATTGCTG CATCGGGTGG GGAAGTTGAA GATTCCTCAC CTTCGGGAGC TTGGGCTGCA 
TCATCCACAG AGAAAACAGG AGAAACAGAA GTGGGCTTTG TACCAACATT TGGACCTTGT 
TATCTAAATC TTTATGGAAG TCCAAGAGAA TACACTGGAT TCCCAGATCC ATATGATGAC 
TTGAATTCTG GAAAGGGTGA AGGAGTTGCC TACAGAGGTC GAATCTTGGT AGAACTGAAT 
ACTTTACTTG AGAAAACACC CCCTGACAAG AAGGTTGAAA CCATATCCAA TGATGATTTG 
CTGGTTGTTG AGAAATATCA GCGTAGGCGA AAATATAGCC TGTCTGCGGT ATTTCATTCT 
GCAACCATGC TGCAAGATAT TGGAGAGGCC ATTCAGTTTG AAGTCAGCAT TGGAAATTAC 
GGCAATAAGT TTGATGCTAC CTGTAAGCCT TTGGCATCGA CAACTCAATA CAGTCGGGCT 
GTGTTTGATG GAAATTTCTA TTATTATTTG CCATGGGCCC AGAAGAAGCC AGTGGTGACT 
CTAACTTCTT ATTGGGAAGA CATTAGTCAC AGACTGGATG CAGTGAACAC CATTCTTGTT 
ATGGCAGAAC GGCTGCACGT GAACATTGAG AATTTAAAAT CAGGAATACA AGCCAAAATG 
TCTGGGAATC ATTTGGCTGA AATGTGGCTG AAGTTGATTG ATGAACTTAT CGAAGATACA 
AGCATGCCAT TGCCTTCACT AGAAGGGAAA TCTAATGTCA CCATTCTAGA CACTCAGATC 
CAAAAGCTGC GTCTTCGATC CCTTATCCAG ATTCAAGAAG CAGCTAGAAA AATGAGGTCT 
GAAGCCAAAC ATGTGAAGTC TACTTTGACA GAAATTGAAG ATTGGTTTGA TAAGCTAATG 
CAACTTTCTG AAGAGCCACA AAACAGTATG CCCGATATTA TCATATGGAT GATCCGAGGA 
GAGAAAAGAT TGGCCTATGC TAGAATTCCT GCACACCAGG TTTTATATTC TACTACCAGT 
GAAGAAGCTT CTGGAAAGTA CTGTGGGAAG ACCCAGACCA TCTTTTTGAA GTACCCACAG 
GACAGGAGCT ATGGTGTGAA GGTTCCAGCA GAATTGCGGG TTAACATTTG GCTTGGATTA 
AGTGCTGTTG AAAAGAAGTT TAACAGCTTT GCTGAAGGAA CATTCACTGT CTTTGCAGAA 
ATGTATGAAA ATCAAGCTTT GATGTTTGGA AAATGGGGCA CTTCTGGACT TGTGGGTCGA 
CATAAATTTT CAGATGTCAC AGGCAAGATA AAGCTCAAGA AAGAGTTCTT TTTGCCCCCT 
AAAGGCTGGG AATGGGAAGG TGAATGGATG GTGGATCCTG AAAGAAGTTT ATTGACTGAA 
GCGGATGCTG GCCACACACA CTTTACAGAT GAAGTGTATC AGAATGAAAG CCGCTATCCA 
GGTGGAGAAT GGAAGGCAAT GGAGGACAGC TACACAGATG TGAATGGAGA AAAAGCAGCA 
TCTCCTAATG AGATGACTTG CCCCCCTGGC TGGGAATGGG AAGATGATGC TTGGATGTAT 
GATATAAACC GAGCTGTAGA TGAAAAAGGC TGGGAATATG GAATTACTAT TCCTCCTGAT 
AACAAGCCAA AGTCCTGGGT TGCTGCAGAG AAGATGTATC ATACACACAG GCGGCGGCGA 
ATGGTCCGCA AACGCAAAAA GGATGTGACA CAGTCGGCTG CAAGTACTGC AAGGGGGTGG 
GAAGCACTAG AAGATAAAGA AGGCTGGGAA TATGCATCTT TAATTGGATG GAAATTTCAC 
TGGAAACAGC GTAGTTCAGA TACTTTTCGT CGAAGACGCT GGAGAAGAAA GATGGCTCCT 
TCTGAAACAC ATGGTTCAGC TGCTATCTTT AAACTTGAAG GTGCCATTGG AGCAGACACT 
ACTGAAGATG GAGATGAAAA GAGCACGGAG AAAAACAAAC ATAGTGCAAC AACCGTGTTT 
GGTGCAAACA CTCCAATTGT TTCCTGTAAT TTTGACAGAG TATATAATTA TCATCTTCGC 
TGCTATATAT ATCAAGCTCG AAATCTTATG GCTTTAGATA AAGATAGTTT TTCAGATCCC 
TATGCTCATG TTTCATTCCT CCATCGGAGC CAAACAACTG AAATCATCCA CTCAACACTG 
AATCCAACCT GGGACCAAAC TCTAATATTT GATGACATTG AAATTTTTGG AGAACCACAA 
ACAATCATAC AAAACCCACC CAAAGTTGTC ATTGAACTCT TTGACAATGA CCAAGTGGGC 
AAGGATGAAT TTTTAGGTCG AAGTTTTTGC TCCCCAGTTG TCAAGCTAAA TCCAGAAGTT 
GACATCACCC CTAAGCTTCT CTGGCAACCA GTCATAAATG GAGATAAGGC TTGTGGGGAT 
GTACTTGTAG CTGCTGAGCT TATTCTCAAA GATAAGGTTG GCTCCAATCT TCCAATTCTC 
CCATCTCAGA GGGCTCCAAA TCTCTACATG GTTCCTCAAG GAATTAGGCC TGTGGTTCAA 
CTCACTGCTA TTGAGATTTT AGCTTGGGGT TTAAGAAACA TGAAAAACTA CCAGATGGCT 
TCTGTGACTT CCCCAAGTCT TATCATAGAG TGTGGAGGAG AAAGGATAGA ATCAGCATTG 
ATCAAAAACC TCAAGAAGAC ACCCAACTTT CCTAGTTCTG TTCTCTTCAT GAAAGTGCTA 
TTGCCCAAGG AAGAATTGTA CACACCACCA TTGGTTATCA AAGTGATCGA CCACAGACAA 
TTTGGGCGAA AGCCCATCGT GGGTCAGTGC ACCATCGACC TTCTTGAACA GTTTCGTTGT 
GACCCTTATG CATTTAAGGA AGATATTGCA CCCCAGGTAA AAGCTTCTAT CTTGTCTGCC 
CCACTTAGCC GGGATGTGGT TATTGAAGTT GAGGACACAA AACCATTGCT TCCAACCAAG 
TTAACTGAAA AGGAGGAAGA AGTTGTTGAC TGGTGGAGCA AATTTTATGC ATCTGTTGGA 
GAACCTGAAA AGTGTGGGCA ATATATCCAG AAGGAATATG CCAAACTAAA GGTATATAAT 
TGTGAACTAG AGAGTGTTCC AGAGTTTAAG GGTCTGACAG ACTTTTCAGA TACCTTTAAG 
CTTTACAGAG GGAAGTCTGA TGAGATTGAT GATCCTTCAG TGGTAGGAGA GTTCAAGGGC 
TCCTTTAGAA TCTATCCACT GCCTGATGAT CCAAATGTGC CAGCTCCTCC CAGGCAGTTT 
CGGGAATTAC CTGACAGTGT CCCACAGGAA TGCATTGTGA GGATTTATAT TGTTCGGGGC 
ATAGATCTCC AGCCTCAGGA TAACAATGGC CTGTGTGACC CTTATATAAA AATATCCCTG 
AGTAAAAAAG TCATTGAAGA TCGAGATAAT TACATTCCAA ATACACTCAA CCCAATTTTT 
GGAAGGATGT ATGAGCTAAG CTGCTTCTTA CCTCAAGAAA AAGACCTGAA AATTTCTGTC 
TATGATTATG ACACACTTAC TCGTGATGAA AAAGTAGGAG AAACTATCAT TGATCTAGAA 
AATCGCTTCC TTTCCCGATT TGGGTCGAAT TGTGGCATAG CAGAGCAATA TTGCATTTCT 
GGAGTCAATA CTTGGAGAGA TCAACTAAAA CCAACCCAAT TGTTATGCAA TGTAGCTAAG 
TTTAAAGGAT TGCCACTCCC TATTCTTTCT GAAGATGGAA ATAGGCTAAG ATTTGGAGGT 
CGAGAGTACA CCTTGGAAGA ATTTGAGGCC AACAGAAATC TGCACCAGCA CCTGGGACCT 
GCAGAGGAGC GTCTTGCTCT GTATATCCTT CGGACTCAGG GTCAAGTCCC TGAGCATGTG 
GAAACAAGGA CTTTGTACAG CACCTTCCAA CCAAATATTT CACAGGGAAA ACTTCAGATG 
TGGGTGGATG TTTTCCCCAA AAGTTTGGGG CCACCAGGCC CTCCTTTCAA TATCACTCCC 
CGGAAGGCCA AGAGGTATGT CCTACGTGTG ATCATTTGGA ACACTAAAGA TGTCATTCTT 
GATGAGAAAA GTATCACAGG AGAAGAGATG ACTGACATCT ATGTGAAAGG CTGGATTCCT 
GGGAATGAAG AAAACAAGCA GAAGACAGAT GTCCATTACA GGTCATTGGA TGGTGAAGGA 
AACTTTAATT GGAGATTTGT TTTCCCTTTT GAATACCTGC CTGCTGAACA ACTCTGTATT 
GTTTCTAAAA AAGAACATTT CTGGAGTATT GACCAGACTG AATTTCGAGT TCCTCCCAGA 
CTCATCATTC AGATATGGGA CAATGATAAG TTTTCTTTAG ATGACTACTT GGGTTTTCTG 
GAACTTGATT TACACAACAC AATTATCCCT GCAAAGTCTT CAGAAAAGTG CAAATTGGAC 
ATGATTTCAG AGTATAGGGC AGTGAATCCT CTAAAGTCCA AGACAGCCTC ACTTTTTGAA 
CAGAAATCGA TGAAAGGATG GTGGCCATGC TATGCAGAAA AAGATGGCGC ACGTGTGATG 
ACTGGTAAAG TGGAGATGAC TTTGGAAGTC CTCAATGAGA AGGAGGTTGA TGAAAGACCA 
GCTGGGAAAG GAAGGGATGA GCCCAACATG AACCCCAAGC TAGAATTACC AAATCGACCA 
GAAACTTCAT TCCTTTGGTT TACCAACCCA TGCAAGACAA TGAAATTCAT CGTGTGGCGT 
CGATTTAAAT GGGTCATCAT TGGCTTACTC ATCCTGCTGG TCTTGTTGCT GTTTTTGGCA 
GTACTCCTAT ATTCTCTGCC GAACTATATA TCAATGAAGA TCGTGAGACC AAATGCAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_016426160.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016281646.1 Monodelphis domestica myoferlin (MYOF), transcript variant X5, mRNA.

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CloneID	OMc38448
Clone ID Related Accession (Same CDS sequence)	XM_016426160.1
Accession Version	XM_016426160.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	myoferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGGG TCATTGTGGA GTCTGCGAGT GATATCCCTA AAGGGAAATT TGGAAAACCG 
GATCCAATTG TCTCAGTTAT TTTTAAGGAT GAGAAGAAGA AAACGAAGAA AATTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATATTT GAATTTGATT TGAAGGGCAT ACCATTGGAC 
TTTTCATCTA CTCTTGGCAT CACTGTGAAA GATTTCGAGA CAATTGGACA AAATAAACTG 
ATTGGCGTTG CAACAATATC ACTGAAGGAC TTGATAGGTG GCCAGAGTCG ATCTCTTCCT 
TATAAACTCA TATCTTTGGT AAATGAAAAG GGTCAAGATA CTGGGGCTAC AATCGATCTG 
GTGGTTGCCT ATGATCCACC AGCAGCCATG GCAACAAATC CAAATGAACT AAGTGGGATC 
ACCATAGAAG GCACGGGAGA AGAAGAGGAA GAAGACACAG GAGATGAAGA TACTGCATTC 
AAAGTCATTG CCCCAAGGGT TCCAGCAACA AATATTTCTG AAGCCCAGCT GGCCAGGAGG 
ATTACAAAAG GAAAGAATAC CCGAAAGATC CTTTCCAATA AGCCACAAGA CTTCCAGATT 
CGTGTTAGAG TGATTGAAGG GCGCCAATTA AGTGGGAATA ACATCAAACC AGTGGTCAAA 
GTCCATGTCT GTGGTCAGAC ACATCGAACA AGAATAAAAA GAGGGAATAA TCCATTTTTT 
GACGAATTAT TTTTCTACAA TGTCCACATG ACCCCTCTTG AACTGGTTGA TGAGACAATA 
AGCATTCGGG TATATGATTC CCATTCTTTG AGAGCAGACT GTCTGATGGG AGAATTTAAG 
ATTGATATTG GTTACATTTA TGATGAACCT GGTCATGCTG TCATGAGAAA ATGGCTTCTT 
CTAAATGACC CTGAAGATAT AAGTTCTGGT GCTAAAGGTT ACATGAAAGT CAGCATGTTT 
GTCCTTGGAA CAGGAGATGA ACCACCAACA GAGAAGAGAG ATCGAGATAA TGACAGTGAT 
GATGTGGAGA GTAATTTGTT ACTTCCTGCT GGAATTGCCA TGCGGTGGGT CACTTTCCTC 
CTGAAAATTT ACAGAGCTGA GGATATTCCT CAGATGGATG ATGCCTTTTC ACAGACTGTC 
AAAGAAATAT TTGGAGCCAA TGTAGACAAG AAAAACTTAG TGGATCCATT TGTAGAAGTT 
TGTTTTGCTG GGAAAAAGGT TTCCACAGGC ATAATAGAGA AAAATGCAAA TCCAGAGTGG 
AATCAAGTTG TCAATCTTCA AATCAAGTTT CCTTCAATGT GTGAAAAAAT AAAGTTAACA 
GTATATGACT GGGACCGCCT TACCAAGAAT GATGTAGTAG GGACAACCTA TCTGTATCTT 
TCTAAAATTG CTGCATCGGG TGGGGAAGTT GAAGATTCCT CACCTTCGGG AGCTTGGGCT 
GCATCATCCA CAGAGAAAAC AGGAGAAACA GAAGTGGGCT TTGTACCAAC ATTTGGACCT 
TGTTATCTAA ATCTTTATGG AAGTCCAAGA GAATACACTG GATTCCCAGA TCCATATGAT 
GACTTGAATT CTGGAAAGGG TGAAGGAGTT GCCTACAGAG GTCGAATCTT GGTAGAACTG 
AATACTTTAC TTGAGAAAAC ACCCCCTGAC AAGAAGGTTG AAACCATATC CAATGATGAT 
TTGCTGGTTG TTGAGAAATA TCAGCGTAGG CGAAAATATA GCCTGTCTGC GGTATTTCAT 
TCTGCAACCA TGCTGCAAGA TATTGGAGAG GCCATTCAGT TTGAAGTCAG CATTGGAAAT 
TACGGCAATA AGTTTGATGC TACCTGTAAG CCTTTGGCAT CGACAACTCA ATACAGTCGG 
GCTGTGTTTG ATGGAAATTT CTATTATTAT TTGCCATGGG CCCAGAAGAA GCCAGTGGTG 
ACTCTAACTT CTTATTGGGA AGACATTAGT CACAGACTGG ATGCAGTGAA CACCATTCTT 
GTTATGGCAG AACGGCTGCA CGTGAACATT GAGAATTTAA AATCAGGAAT ACAAGCCAAA 
ATGTCTGGGA ATCATTTGGC TGAAATGTGG CTGAAGTTGA TTGATGAACT TATCGAAGAT 
ACAAGCATGC CATTGCCTTC ACTAGAAGGG AAATCTAATG TCACCATTCT AGACACTCAG 
ATCCAAAAGC TGCGTCTTCG ATCCCTTATC CAGATTCAAG AAGCAGCTAG AAAAATGAGG 
TCTGAAGCCA AACATGTGAA GTCTACTTTG ACAGAAATTG AAGATTGGTT TGATAAGCTA 
ATGCAACTTT CTGAAGAGCC ACAAAACAGT ATGCCCGATA TTATCATATG GATGATCCGA 
GGAGAGAAAA GATTGGCCTA TGCTAGAATT CCTGCACACC AGGTTTTATA TTCTACTACC 
AGTGAAGAAG CTTCTGGAAA GTACTGTGGG AAGACCCAGA CCATCTTTTT GAAGTACCCA 
CAGGACAGGA GCTATGGTGT GAAGGTTCCA GCAGAATTGC GGGTTAACAT TTGGCTTGGA 
TTAAGTGCTG TTGAAAAGAA GTTTAACAGC TTTGCTGAAG GAACATTCAC TGTCTTTGCA 
GAAATGTATG AAAATCAAGC TTTGATGTTT GGAAAATGGG GCACTTCTGG ACTTGTGGGT 
CGACATAAAT TTTCAGATGT CACAGGCAAG ATAAAGCTCA AGAAAGAGTT CTTTTTGCCC 
CCTAAAGGCT GGGAATGGGA AGGTGAATGG ATGGTGGATC CTGAAAGAAG TTTATTGACT 
GAAGCGGATG CTGGCCACAC ACACTTTACA GATGAAGTGT ATCAGAATGA AAGCCGCTAT 
CCAGGTGGAG AATGGAAGGC AATGGAGGAC AGCTACACAG ATGTGAATGG AGAAAAAGCA 
GCATCTCCTA ATGAGATGAC TTGCCCCCCT GGCTGGGAAT GGGAAGATGA TGCTTGGATG 
TATGATATAA ACCGAGCTGT AGATGAAAAA GGCTGGGAAT ATGGAATTAC TATTCCTCCT 
GATAACAAGC CAAAGTCCTG GGTTGCTGCA GAGAAGATGT ATCATACACA CAGGCGGCGG 
CGAATGGTCC GCAAACGCAA AAAGGATGTG ACACAGTCGG CTGCAAGTAC TGCAAGGGGG 
TGGGAAGCAC TAGAAGATAA AGAAGGCTGG GAATATGCAT CTTTAATTGG ATGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACTTTT CGTCGAAGAC GCTGGAGAAG AAAGATGGCT 
CCTTCTGAAA CACATGGTTC AGCTGCTATC TTTAAACTTG AAGGTGCCAT TGGAGCAGAC 
ACTACTGAAG ATGGAGATGA AAAGAGCACG GAGAAAAACA AACATAGTGC AACAACCGTG 
TTTGGTGCAA ACACTCCAAT TGTTTCCTGT AATTTTGACA GAGTATATAA TTATCATCTT 
CGCTGCTATA TATATCAAGC TCGAAATCTT ATGGCTTTAG ATAAAGATAG TTTTTCAGAT 
CCCTATGCTC ATGTTTCATT CCTCCATCGG AGCCAAACAA CTGAAATCAT CCACTCAACA 
CTGAATCCAA CCTGGGACCA AACTCTAATA TTTGATGACA TTGAAATTTT TGGAGAACCA 
CAAACAATCA TACAAAACCC ACCCAAAGTT GTCATTGAAC TCTTTGACAA TGACCAAGTG 
GGCAAGGATG AATTTTTAGG TCGAAGTTTT TGCTCCCCAG TTGTCAAGCT AAATCCAGAA 
GTTGACATCA CCCCTAAGCT TCTCTGGCAA CCAGTCATAA ATGGAGATAA GGCTTGTGGG 
GATGTACTTG TAGCTGCTGA GCTTATTCTC AAAGATAAGG TTGGCTCCAA TCTTCCAATT 
CTCCCATCTC AGAGGGCTCC AAATCTCTAC ATGGTTCCTC AAGGAATTAG GCCTGTGGTT 
CAACTCACTG CTATTGAGAT TTTAGCTTGG GGTTTAAGAA ACATGAAAAA CTACCAGATG 
GCTTCTGTGA CTTCCCCAAG TCTTATCATA GAGTGTGGAG GAGAAAGGAT AGAATCAGCA 
TTGATCAAAA ACCTCAAGAA GACACCCAAC TTTCCTAGTT CTGTTCTCTT CATGAAAGTG 
CTATTGCCCA AGGAAGAATT GTACACACCA CCATTGGTTA TCAAAGTGAT CGACCACAGA 
CAATTTGGGC GAAAGCCCAT CGTGGGTCAG TGCACCATCG ACCTTCTTGA ACAGTTTCGT 
TGTGACCCTT ATGCATTTAA GGAAGATATT GCACCCCAGG TAAAAGCTTC TATCTTGTCT 
GCCCCACTTA GCCGGGATGT GGTTATTGAA GTTGAGGACA CAAAACCATT GCTTCCAACC 
AAGTTCTCGG TATCTCCATC CTCTTGCCAT TTGTGTGGCT CTCTGTCCTG CTGTTGCACA 
CGTGTCCATG ATCAGTTAAC TGAAAAGGAG GAAGAAGTTG TTGACTGGTG GAGCAAATTT 
TATGCATCTG TTGGAGAACC TGAAAAGTGT GGGCAATATA TCCAGAAGGA ATATGCCAAA 
CTAAAGGTAT ATAATTGTGA ACTAGAGAGT GTTCCAGAGT TTAAGGGTCT GACAGACTTT 
TCAGATACCT TTAAGCTTTA CAGAGGGAAG TCTGATGAGA TTGATGATCC TTCAGTGGTA 
GGAGAGTTCA AGGGCTCCTT TAGAATCTAT CCACTGCCTG ATGATCCAAA TGTGCCAGCT 
CCTCCCAGGC AGTTTCGGGA ATTACCTGAC AGTGTCCCAC AGGAATGCAT TGTGAGGATT 
TATATTGTTC GGGGCATAGA TCTCCAGCCT CAGGATAACA ATGGCCTGTG TGACCCTTAT 
ATAAAAATAT CCCTGAGTAA AAAAGTCATT GAAGATCGAG ATAATTACAT TCCAAATACA 
CTCAACCCAA TTTTTGGAAG GATGTATGAG CTAAGCTGCT TCTTACCTCA AGAAAAAGAC 
CTGAAAATTT CTGTCTATGA TTATGACACA CTTACTCGTG ATGAAAAAGT AGGAGAAACT 
ATCATTGATC TAGAAAATCG CTTCCTTTCC CGATTTGGGT CGAATTGTGG CATAGCAGAG 
CAATATTGCA TTTCTGGAGT CAATACTTGG AGAGATCAAC TAAAACCAAC CCAATTGTTA 
TGCAATGTAG CTAAGTTTAA AGGATTGCCA CTCCCTATTC TTTCTGAAGA TGGAAATAGG 
CTAAGATTTG GAGGTCGAGA GTACACCTTG GAAGAATTTG AGGCCAACAG AAATCTGCAC 
CAGCACCTGG GACCTGCAGA GGAGCGTCTT GCTCTGTATA TCCTTCGGAC TCAGGGTCAA 
GTCCCTGAGC ATGTGGAAAC AAGGACTTTG TACAGCACCT TCCAACCAAA TATTTCACAG 
GGAAAACTTC AGATGTGGGT GGATGTTTTC CCCAAAAGTT TGGGGCCACC AGGCCCTCCT 
TTCAATATCA CTCCCCGGAA GGCCAAGAGG TATGTCCTAC GTGTGATCAT TTGGAACACT 
AAAGATGTCA TTCTTGATGA GAAAAGTATC ACAGGAGAAG AGATGACTGA CATCTATGTG 
AAAGGCTGGA TTCCTGGGAA TGAAGAAAAC AAGCAGAAGA CAGATGTCCA TTACAGGTCA 
TTGGATGGTG AAGGAAACTT TAATTGGAGA TTTGTTTTCC CTTTTGAATA CCTGCCTGCT 
GAACAACTCT GTATTGTTTC TAAAAAAGAA CATTTCTGGA GTATTGACCA GACTGAATTT 
CGAGTTCCTC CCAGACTCAT CATTCAGATA TGGGACAATG ATAAGTTTTC TTTAGATGAC 
TACTTGGGTT TTCTGGAACT TGATTTACAC AACACAATTA TCCCTGCAAA GTCTTCAGAA 
AAGTGCAAAT TGGACATGAT TTCAGAGTAT AGGGCAGTGA ATCCTCTAAA GTCCAAGACA 
GCCTCACTTT TTGAACAGAA ATCGATGAAA GGATGGTGGC CATGCTATGC AGAAAAAGAT 
GGCGCACGTG TGATGACTGG TAAAGTGGAG ATGACTTTGG AAGTCCTCAA TGAGAAGGAG 
GTTGATGAAA GACCAGCTGG GAAAGGAAGG GATGAGCCCA ACATGAACCC CAAGCTAGAA 
TTACCAAATC GACCAGAAAC TTCATTCCTT TGGTTTACCA ACCCATGCAA GACAATGAAA 
TTCATCGTGT GGCGTCGATT TAAATGGGTC ATCATTGGCT TACTCATCCT GCTGGTCTTG 
TTGCTGTTTT TGGCAGTACT CCTATATTCT CTGCCGAACT ATATATCAAT GAAGATCGTG 
AGACCAAATG CAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016281646.1
CDS	181..6438
Translation	MLRVIVESASDIPKGKFGKPDPIVSVIFKDEKKKTKKIDNELNPVWNEIFEFDLKGIPLDFSSTLGITVKDFETIGQNKLIGVATISLKDLIGGQSRSLPYKLISLVNEKGQDTGATIDLVVAYDPPAAMATNPNELSGITIEGTGEEEEEDTGDEDTAFKVIAPRVPATNISEAQLARRITKGKNTRKILSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHMTPLELVDETISIRVYDSHSLRADCLMGEFKIDIGYIYDEPGHAVMRKWLLLNDPEDISSGAKGYMKVSMFVLGTGDEPPTEKRDRDNDSDDVESNLLLPAGIAMRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGANVDKKNLVDPFVEVCFAGKKVSTGIIEKNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEDSSPSGAWAASSTEKTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDDLNSGKGEGVAYRGRILVELNTLLEKTPPDKKVETISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNFYYYLPWAQKKPVVTLTSYWEDISHRLDAVNTILVMAERLHVNIENLKSGIQAKMSGNHLAEMWLKLIDELIEDTSMPLPSLEGKSNVTILDTQIQKLRLRSLIQIQEAARKMRSEAKHVKSTLTEIEDWFDKLMQLSEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTTSEEASGKYCGKTQTIFLKYPQDRSYGVKVPAELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKKEFFLPPKGWEWEGEWMVDPERSLLTEADAGHTHFTDEVYQNESRYPGGEWKAMEDSYTDVNGEKAASPNEMTCPPGWEWEDDAWMYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRMVRKRKKDVTQSAASTARGWEALEDKEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGSAAIFKLEGAIGADTTEDGDEKSTEKNKHSATTVFGANTPIVSCNFDRVYNYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSQTTEIIHSTLNPTWDQTLIFDDIEIFGEPQTIIQNPPKVVIELFDNDQVGKDEFLGRSFCSPVVKLNPEVDITPKLLWQPVINGDKACGDVLVAAELILKDKVGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIIECGGERIESALIKNLKKTPNFPSSVLFMKVLLPKEELYTPPLVIKVIDHRQFGRKPIVGQCTIDLLEQFRCDPYAFKEDIAPQVKASILSAPLSRDVVIEVEDTKPLLPTKFSVSPSSCHLCGSLSCCCTRVHDQLTEKEEEVVDWWSKFYASVGEPEKCGQYIQKEYAKLKVYNCELESVPEFKGLTDFSDTFKLYRGKSDEIDDPSVVGEFKGSFRIYPLPDDPNVPAPPRQFRELPDSVPQECIVRIYIVRGIDLQPQDNNGLCDPYIKISLSKKVIEDRDNYIPNTLNPIFGRMYELSCFLPQEKDLKISVYDYDTLTRDEKVGETIIDLENRFLSRFGSNCGIAEQYCISGVNTWRDQLKPTQLLCNVAKFKGLPLPILSEDGNRLRFGGREYTLEEFEANRNLHQHLGPAEERLALYILRTQGQVPEHVETRTLYSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKRYVLRVIIWNTKDVILDEKSITGEEMTDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFEYLPAEQLCIVSKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHNTIIPAKSSEKCKLDMISEYRAVNPLKSKTASLFEQKSMKGWWPCYAEKDGARVMTGKVEMTLEVLNEKEVDERPAGKGRDEPNMNPKLELPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLILLVLLLFLAVLLYSLPNYISMKIVRPNAK

Target ORF information:

RefSeq Version	XM_016426160.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica myoferlin (MYOF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016426160.1

ORF Insert Sequence:

ATGCTTCGGG TCATTGTGGA GTCTGCGAGT GATATCCCTA AAGGGAAATT TGGAAAACCG 
GATCCAATTG TCTCAGTTAT TTTTAAGGAT GAGAAGAAGA AAACGAAGAA AATTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATATTT GAATTTGATT TGAAGGGCAT ACCATTGGAC 
TTTTCATCTA CTCTTGGCAT CACTGTGAAA GATTTCGAGA CAATTGGACA AAATAAACTG 
ATTGGCGTTG CAACAATATC ACTGAAGGAC TTGATAGGTG GCCAGAGTCG ATCTCTTCCT 
TATAAACTCA TATCTTTGGT AAATGAAAAG GGTCAAGATA CTGGGGCTAC AATCGATCTG 
GTGGTTGCCT ATGATCCACC AGCAGCCATG GCAACAAATC CAAATGAACT AAGTGGGATC 
ACCATAGAAG GCACGGGAGA AGAAGAGGAA GAAGACACAG GAGATGAAGA TACTGCATTC 
AAAGTCATTG CCCCAAGGGT TCCAGCAACA AATATTTCTG AAGCCCAGCT GGCCAGGAGG 
ATTACAAAAG GAAAGAATAC CCGAAAGATC CTTTCCAATA AGCCACAAGA CTTCCAGATT 
CGTGTTAGAG TGATTGAAGG GCGCCAATTA AGTGGGAATA ACATCAAACC AGTGGTCAAA 
GTCCATGTCT GTGGTCAGAC ACATCGAACA AGAATAAAAA GAGGGAATAA TCCATTTTTT 
GACGAATTAT TTTTCTACAA TGTCCACATG ACCCCTCTTG AACTGGTTGA TGAGACAATA 
AGCATTCGGG TATATGATTC CCATTCTTTG AGAGCAGACT GTCTGATGGG AGAATTTAAG 
ATTGATATTG GTTACATTTA TGATGAACCT GGTCATGCTG TCATGAGAAA ATGGCTTCTT 
CTAAATGACC CTGAAGATAT AAGTTCTGGT GCTAAAGGTT ACATGAAAGT CAGCATGTTT 
GTCCTTGGAA CAGGAGATGA ACCACCAACA GAGAAGAGAG ATCGAGATAA TGACAGTGAT 
GATGTGGAGA GTAATTTGTT ACTTCCTGCT GGAATTGCCA TGCGGTGGGT CACTTTCCTC 
CTGAAAATTT ACAGAGCTGA GGATATTCCT CAGATGGATG ATGCCTTTTC ACAGACTGTC 
AAAGAAATAT TTGGAGCCAA TGTAGACAAG AAAAACTTAG TGGATCCATT TGTAGAAGTT 
TGTTTTGCTG GGAAAAAGGT TTCCACAGGC ATAATAGAGA AAAATGCAAA TCCAGAGTGG 
AATCAAGTTG TCAATCTTCA AATCAAGTTT CCTTCAATGT GTGAAAAAAT AAAGTTAACA 
GTATATGACT GGGACCGCCT TACCAAGAAT GATGTAGTAG GGACAACCTA TCTGTATCTT 
TCTAAAATTG CTGCATCGGG TGGGGAAGTT GAAGATTCCT CACCTTCGGG AGCTTGGGCT 
GCATCATCCA CAGAGAAAAC AGGAGAAACA GAAGTGGGCT TTGTACCAAC ATTTGGACCT 
TGTTATCTAA ATCTTTATGG AAGTCCAAGA GAATACACTG GATTCCCAGA TCCATATGAT 
GACTTGAATT CTGGAAAGGG TGAAGGAGTT GCCTACAGAG GTCGAATCTT GGTAGAACTG 
AATACTTTAC TTGAGAAAAC ACCCCCTGAC AAGAAGGTTG AAACCATATC CAATGATGAT 
TTGCTGGTTG TTGAGAAATA TCAGCGTAGG CGAAAATATA GCCTGTCTGC GGTATTTCAT 
TCTGCAACCA TGCTGCAAGA TATTGGAGAG GCCATTCAGT TTGAAGTCAG CATTGGAAAT 
TACGGCAATA AGTTTGATGC TACCTGTAAG CCTTTGGCAT CGACAACTCA ATACAGTCGG 
GCTGTGTTTG ATGGAAATTT CTATTATTAT TTGCCATGGG CCCAGAAGAA GCCAGTGGTG 
ACTCTAACTT CTTATTGGGA AGACATTAGT CACAGACTGG ATGCAGTGAA CACCATTCTT 
GTTATGGCAG AACGGCTGCA CGTGAACATT GAGAATTTAA AATCAGGAAT ACAAGCCAAA 
ATGTCTGGGA ATCATTTGGC TGAAATGTGG CTGAAGTTGA TTGATGAACT TATCGAAGAT 
ACAAGCATGC CATTGCCTTC ACTAGAAGGG AAATCTAATG TCACCATTCT AGACACTCAG 
ATCCAAAAGC TGCGTCTTCG ATCCCTTATC CAGATTCAAG AAGCAGCTAG AAAAATGAGG 
TCTGAAGCCA AACATGTGAA GTCTACTTTG ACAGAAATTG AAGATTGGTT TGATAAGCTA 
ATGCAACTTT CTGAAGAGCC ACAAAACAGT ATGCCCGATA TTATCATATG GATGATCCGA 
GGAGAGAAAA GATTGGCCTA TGCTAGAATT CCTGCACACC AGGTTTTATA TTCTACTACC 
AGTGAAGAAG CTTCTGGAAA GTACTGTGGG AAGACCCAGA CCATCTTTTT GAAGTACCCA 
CAGGACAGGA GCTATGGTGT GAAGGTTCCA GCAGAATTGC GGGTTAACAT TTGGCTTGGA 
TTAAGTGCTG TTGAAAAGAA GTTTAACAGC TTTGCTGAAG GAACATTCAC TGTCTTTGCA 
GAAATGTATG AAAATCAAGC TTTGATGTTT GGAAAATGGG GCACTTCTGG ACTTGTGGGT 
CGACATAAAT TTTCAGATGT CACAGGCAAG ATAAAGCTCA AGAAAGAGTT CTTTTTGCCC 
CCTAAAGGCT GGGAATGGGA AGGTGAATGG ATGGTGGATC CTGAAAGAAG TTTATTGACT 
GAAGCGGATG CTGGCCACAC ACACTTTACA GATGAAGTGT ATCAGAATGA AAGCCGCTAT 
CCAGGTGGAG AATGGAAGGC AATGGAGGAC AGCTACACAG ATGTGAATGG AGAAAAAGCA 
GCATCTCCTA ATGAGATGAC TTGCCCCCCT GGCTGGGAAT GGGAAGATGA TGCTTGGATG 
TATGATATAA ACCGAGCTGT AGATGAAAAA GGCTGGGAAT ATGGAATTAC TATTCCTCCT 
GATAACAAGC CAAAGTCCTG GGTTGCTGCA GAGAAGATGT ATCATACACA CAGGCGGCGG 
CGAATGGTCC GCAAACGCAA AAAGGATGTG ACACAGTCGG CTGCAAGTAC TGCAAGGGGG 
TGGGAAGCAC TAGAAGATAA AGAAGGCTGG GAATATGCAT CTTTAATTGG ATGGAAATTT 
CACTGGAAAC AGCGTAGTTC AGATACTTTT CGTCGAAGAC GCTGGAGAAG AAAGATGGCT 
CCTTCTGAAA CACATGGTTC AGCTGCTATC TTTAAACTTG AAGGTGCCAT TGGAGCAGAC 
ACTACTGAAG ATGGAGATGA AAAGAGCACG GAGAAAAACA AACATAGTGC AACAACCGTG 
TTTGGTGCAA ACACTCCAAT TGTTTCCTGT AATTTTGACA GAGTATATAA TTATCATCTT 
CGCTGCTATA TATATCAAGC TCGAAATCTT ATGGCTTTAG ATAAAGATAG TTTTTCAGAT 
CCCTATGCTC ATGTTTCATT CCTCCATCGG AGCCAAACAA CTGAAATCAT CCACTCAACA 
CTGAATCCAA CCTGGGACCA AACTCTAATA TTTGATGACA TTGAAATTTT TGGAGAACCA 
CAAACAATCA TACAAAACCC ACCCAAAGTT GTCATTGAAC TCTTTGACAA TGACCAAGTG 
GGCAAGGATG AATTTTTAGG TCGAAGTTTT TGCTCCCCAG TTGTCAAGCT AAATCCAGAA 
GTTGACATCA CCCCTAAGCT TCTCTGGCAA CCAGTCATAA ATGGAGATAA GGCTTGTGGG 
GATGTACTTG TAGCTGCTGA GCTTATTCTC AAAGATAAGG TTGGCTCCAA TCTTCCAATT 
CTCCCATCTC AGAGGGCTCC AAATCTCTAC ATGGTTCCTC AAGGAATTAG GCCTGTGGTT 
CAACTCACTG CTATTGAGAT TTTAGCTTGG GGTTTAAGAA ACATGAAAAA CTACCAGATG 
GCTTCTGTGA CTTCCCCAAG TCTTATCATA GAGTGTGGAG GAGAAAGGAT AGAATCAGCA 
TTGATCAAAA ACCTCAAGAA GACACCCAAC TTTCCTAGTT CTGTTCTCTT CATGAAAGTG 
CTATTGCCCA AGGAAGAATT GTACACACCA CCATTGGTTA TCAAAGTGAT CGACCACAGA 
CAATTTGGGC GAAAGCCCAT CGTGGGTCAG TGCACCATCG ACCTTCTTGA ACAGTTTCGT 
TGTGACCCTT ATGCATTTAA GGAAGATATT GCACCCCAGG TAAAAGCTTC TATCTTGTCT 
GCCCCACTTA GCCGGGATGT GGTTATTGAA GTTGAGGACA CAAAACCATT GCTTCCAACC 
AAGTTCTCGG TATCTCCATC CTCTTGCCAT TTGTGTGGCT CTCTGTCCTG CTGTTGCACA 
CGTGTCCATG ATCAGTTAAC TGAAAAGGAG GAAGAAGTTG TTGACTGGTG GAGCAAATTT 
TATGCATCTG TTGGAGAACC TGAAAAGTGT GGGCAATATA TCCAGAAGGA ATATGCCAAA 
CTAAAGGTAT ATAATTGTGA ACTAGAGAGT GTTCCAGAGT TTAAGGGTCT GACAGACTTT 
TCAGATACCT TTAAGCTTTA CAGAGGGAAG TCTGATGAGA TTGATGATCC TTCAGTGGTA 
GGAGAGTTCA AGGGCTCCTT TAGAATCTAT CCACTGCCTG ATGATCCAAA TGTGCCAGCT 
CCTCCCAGGC AGTTTCGGGA ATTACCTGAC AGTGTCCCAC AGGAATGCAT TGTGAGGATT 
TATATTGTTC GGGGCATAGA TCTCCAGCCT CAGGATAACA ATGGCCTGTG TGACCCTTAT 
ATAAAAATAT CCCTGAGTAA AAAAGTCATT GAAGATCGAG ATAATTACAT TCCAAATACA 
CTCAACCCAA TTTTTGGAAG GATGTATGAG CTAAGCTGCT TCTTACCTCA AGAAAAAGAC 
CTGAAAATTT CTGTCTATGA TTATGACACA CTTACTCGTG ATGAAAAAGT AGGAGAAACT 
ATCATTGATC TAGAAAATCG CTTCCTTTCC CGATTTGGGT CGAATTGTGG CATAGCAGAG 
CAATATTGCA TTTCTGGAGT CAATACTTGG AGAGATCAAC TAAAACCAAC CCAATTGTTA 
TGCAATGTAG CTAAGTTTAA AGGATTGCCA CTCCCTATTC TTTCTGAAGA TGGAAATAGG 
CTAAGATTTG GAGGTCGAGA GTACACCTTG GAAGAATTTG AGGCCAACAG AAATCTGCAC 
CAGCACCTGG GACCTGCAGA GGAGCGTCTT GCTCTGTATA TCCTTCGGAC TCAGGGTCAA 
GTCCCTGAGC ATGTGGAAAC AAGGACTTTG TACAGCACCT TCCAACCAAA TATTTCACAG 
GGAAAACTTC AGATGTGGGT GGATGTTTTC CCCAAAAGTT TGGGGCCACC AGGCCCTCCT 
TTCAATATCA CTCCCCGGAA GGCCAAGAGG TATGTCCTAC GTGTGATCAT TTGGAACACT 
AAAGATGTCA TTCTTGATGA GAAAAGTATC ACAGGAGAAG AGATGACTGA CATCTATGTG 
AAAGGCTGGA TTCCTGGGAA TGAAGAAAAC AAGCAGAAGA CAGATGTCCA TTACAGGTCA 
TTGGATGGTG AAGGAAACTT TAATTGGAGA TTTGTTTTCC CTTTTGAATA CCTGCCTGCT 
GAACAACTCT GTATTGTTTC TAAAAAAGAA CATTTCTGGA GTATTGACCA GACTGAATTT 
CGAGTTCCTC CCAGACTCAT CATTCAGATA TGGGACAATG ATAAGTTTTC TTTAGATGAC 
TACTTGGGTT TTCTGGAACT TGATTTACAC AACACAATTA TCCCTGCAAA GTCTTCAGAA 
AAGTGCAAAT TGGACATGAT TTCAGAGTAT AGGGCAGTGA ATCCTCTAAA GTCCAAGACA 
GCCTCACTTT TTGAACAGAA ATCGATGAAA GGATGGTGGC CATGCTATGC AGAAAAAGAT 
GGCGCACGTG TGATGACTGG TAAAGTGGAG ATGACTTTGG AAGTCCTCAA TGAGAAGGAG 
GTTGATGAAA GACCAGCTGG GAAAGGAAGG GATGAGCCCA ACATGAACCC CAAGCTAGAA 
TTACCAAATC GACCAGAAAC TTCATTCCTT TGGTTTACCA ACCCATGCAA GACAATGAAA 
TTCATCGTGT GGCGTCGATT TAAATGGGTC ATCATTGGCT TACTCATCCT GCTGGTCTTG 
TTGCTGTTTT TGGCAGTACT CCTATATTCT CTGCCGAACT ATATATCAAT GAAGATCGTG 
AGACCAAATG CAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOF ( XM_016426161.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_016281647.1 Monodelphis domestica myoferlin (MYOF), transcript variant X6, mRNA.

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CloneID	OMc38449
Clone ID Related Accession (Same CDS sequence)	XM_016426161.1
Accession Version	XM_016426161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	myoferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008801.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCGGG TCATTGTGGA GTCTGCGAGT GATATCCCTA AAGGGAAATT TGGAAAACCG 
GATCCAATTG TCTCAGTTAT TTTTAAGGAT GAGAAGAAGA AAACGAAGAA AATTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATATTT GAATTTGATT TGAAGGGCAT ACCATTGGAC 
TTTTCATCTA CTCTTGGCAT CACTGTGAAA GATTTCGAGA CAATTGGACA AAATAAACTG 
ATTGGCGTTG CAACAATATC ACTGAAGGAC TTGATAGGTG GCCAGAGTCG ATCTCTTCCT 
TATAAACTCA TATCTTTGGT AAATGAAAAG GGTCAAGATA CTGGGGCTAC AATCGATCTG 
GTGGTTGCCT ATGATCCACC AGCAGCCATG GCAACAAATC CAAATGAACT AAGTGGGATC 
ACCATAGAAG GCACGGGAGA AGAAGAGGAA GAAGACACAG GAGATGAAGA TACTGCATTC 
AAAGTCATTG CCCCAAGGGT TCCAGCAACA AATATTTCTG AAGCCCAGCT GGCCAGGAGG 
ATTACAAAAG GAAAGAATAC CCGAAAGATC CTTTCCAATA AGCCACAAGA CTTCCAGATT 
CGTGTTAGAG TGATTGAAGG GCGCCAATTA AGTGGGAATA ACATCAAACC AGTGGTCAAA 
GTCCATGTCT GTGGTCAGAC ACATCGAACA AGAATAAAAA GAGGGAATAA TCCATTTTTT 
GACGAATTAT TTTTCTACAA TGTCCACATG ACCCCTCTTG AACTGGTTGA TGAGACAATA 
AGCATTCGGG TATATGATTC CCATTCTTTG AGAGCAGACT GTCTGATGGG AGAATTTAAG 
ATTGATATTG GTTACATTTA TGATGAACCT GGTCATGCTG TCATGAGAAA ATGGCTTCTT 
CTAAATGACC CTGAAGATAT AAGTTCTGGT GCTAAAGGTT ACATGAAAGT CAGCATGTTT 
GTCCTTGGAA CAGGAGATGA ACCACCAACA GAGAAGAGAG ATCGAGATAA TGACAGTGAT 
GATGTGGAGA GTAATTTGTT ACTTCCTGCT GGAATTGCCA TGCGGTGGGT CACTTTCCTC 
CTGAAAATTT ACAGAGCTGA GGATATTCCT CAGATGGATG ATGCCTTTTC ACAGACTGTC 
AAAGAAATAT TTGGAGCCAA TGTAGACAAG AAAAACTTAG TGGATCCATT TGTAGAAGTT 
TGTTTTGCTG GGAAAAAGGT TTCCACAGGC ATAATAGAGA AAAATGCAAA TCCAGAGTGG 
AATCAAGTTG TCAATCTTCA AATCAAGTTT CCTTCAATGT GTGAAAAAAT AAAGTTAACA 
GTATATGACT GGGACCGCCT TACCAAGAAT GATGTAGTAG GGACAACCTA TCTGTATCTT 
TCTAAAATTG CTGCATCGGG TGGGGAAGTT GAAGAGAAAA CAGGAGAAAC AGAAGTGGGC 
TTTGTACCAA CATTTGGACC TTGTTATCTA AATCTTTATG GAAGTCCAAG AGAATACACT 
GGATTCCCAG ATCCATATGA TGACTTGAAT TCTGGAAAGG GTGAAGGAGT TGCCTACAGA 
GGTCGAATCT TGGTAGAACT GAATACTTTA CTTGAGAAAA CACCCCCTGA CAAGAAGGTT 
GAAACCATAT CCAATGATGA TTTGCTGGTT GTTGAGAAAT ATCAGCGTAG GCGAAAATAT 
AGCCTGTCTG CGGTATTTCA TTCTGCAACC ATGCTGCAAG ATATTGGAGA GGCCATTCAG 
TTTGAAGTCA GCATTGGAAA TTACGGCAAT AAGTTTGATG CTACCTGTAA GCCTTTGGCA 
TCGACAACTC AATACAGTCG GGCTGTGTTT GATGGAAATT TCTATTATTA TTTGCCATGG 
GCCCAGAAGA AGCCAGTGGT GACTCTAACT TCTTATTGGG AAGACATTAG TCACAGACTG 
GATGCAGTGA ACACCATTCT TGTTATGGCA GAACGGCTGC ACGTGAACAT TGAGAATTTA 
AAATCAGGAA TACAAGCCAA AATGTCTGGG AATCATTTGG CTGAAATGTG GCTGAAGTTG 
ATTGATGAAC TTATCGAAGA TACAAGCATG CCATTGCCTT CACTAGAAGG GAAATCTAAT 
GTCACCATTC TAGACACTCA GATCCAAAAG CTGCGTCTTC GATCCCTTAT CCAGATTCAA 
GAAGCAGCTA GAAAAATGAG GTCTGAAGCC AAACATGTGA AGTCTACTTT GACAGAAATT 
GAAGATTGGT TTGATAAGCT AATGCAACTT TCTGAAGAGC CACAAAACAG TATGCCCGAT 
ATTATCATAT GGATGATCCG AGGAGAGAAA AGATTGGCCT ATGCTAGAAT TCCTGCACAC 
CAGGTTTTAT ATTCTACTAC CAGTGAAGAA GCTTCTGGAA AGTACTGTGG GAAGACCCAG 
ACCATCTTTT TGAAGTACCC ACAGGACAGG AGCTATGGTG TGAAGGTTCC AGCAGAATTG 
CGGGTTAACA TTTGGCTTGG ATTAAGTGCT GTTGAAAAGA AGTTTAACAG CTTTGCTGAA 
GGAACATTCA CTGTCTTTGC AGAAATGTAT GAAAATCAAG CTTTGATGTT TGGAAAATGG 
GGCACTTCTG GACTTGTGGG TCGACATAAA TTTTCAGATG TCACAGGCAA GATAAAGCTC 
AAGAAAGAGT TCTTTTTGCC CCCTAAAGGC TGGGAATGGG AAGGTGAATG GATGGTGGAT 
CCTGAAAGAA GTTTATTGAC TGAAGCGGAT GCTGGCCACA CACACTTTAC AGATGAAGTG 
TATCAGAATG AAAGCCGCTA TCCAGGTGGA GAATGGAAGG CAATGGAGGA CAGCTACACA 
GATGTGAATG GAGAAAAAGC AGCATCTCCT AATGAGATGA CTTGCCCCCC TGGCTGGGAA 
TGGGAAGATG ATGCTTGGAT GTATGATATA AACCGAGCTG TAGATGAAAA AGGCTGGGAA 
TATGGAATTA CTATTCCTCC TGATAACAAG CCAAAGTCCT GGGTTGCTGC AGAGAAGATG 
TATCATACAC ACAGGCGGCG GCGAATGGTC CGCAAACGCA AAAAGGATGT GACACAGTCG 
GCTGCAAGTA CTGCAAGGGG GTGGGAAGCA CTAGAAGATA AAGAAGGCTG GGAATATGCA 
TCTTTAATTG GATGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACTTT TCGTCGAAGA 
CGCTGGAGAA GAAAGATGGC TCCTTCTGAA ACACATGGTT CAGCTGCTAT CTTTAAACTT 
GAAGGTGCCA TTGGAGCAGA CACTACTGAA GATGGAGATG AAAAGAGCAC GGAGAAAAAC 
AAACATAGTG CAACAACCGT GTTTGGTGCA AACACTCCAA TTGTTTCCTG TAATTTTGAC 
AGAGTATATA ATTATCATCT TCGCTGCTAT ATATATCAAG CTCGAAATCT TATGGCTTTA 
GATAAAGATA GTTTTTCAGA TCCCTATGCT CATGTTTCAT TCCTCCATCG GAGCCAAACA 
ACTGAAATCA TCCACTCAAC ACTGAATCCA ACCTGGGACC AAACTCTAAT ATTTGATGAC 
ATTGAAATTT TTGGAGAACC ACAAACAATC ATACAAAACC CACCCAAAGT TGTCATTGAA 
CTCTTTGACA ATGACCAAGT GGGCAAGGAT GAATTTTTAG GTCGAAGTTT TTGCTCCCCA 
GTTGTCAAGC TAAATCCAGA AGTTGACATC ACCCCTAAGC TTCTCTGGCA ACCAGTCATA 
AATGGAGATA AGGCTTGTGG GGATGTACTT GTAGCTGCTG AGCTTATTCT CAAAGATAAG 
GTTGGCTCCA ATCTTCCAAT TCTCCCATCT CAGAGGGCTC CAAATCTCTA CATGGTTCCT 
CAAGGAATTA GGCCTGTGGT TCAACTCACT GCTATTGAGA TTTTAGCTTG GGGTTTAAGA 
AACATGAAAA ACTACCAGAT GGCTTCTGTG ACTTCCCCAA GTCTTATCAT AGAGTGTGGA 
GGAGAAAGGA TAGAATCAGC ATTGATCAAA AACCTCAAGA AGACACCCAA CTTTCCTAGT 
TCTGTTCTCT TCATGAAAGT GCTATTGCCC AAGGAAGAAT TGTACACACC ACCATTGGTT 
ATCAAAGTGA TCGACCACAG ACAATTTGGG CGAAAGCCCA TCGTGGGTCA GTGCACCATC 
GACCTTCTTG AACAGTTTCG TTGTGACCCT TATGCATTTA AGGAAGATAT TGCACCCCAG 
GTAAAAGCTT CTATCTTGTC TGCCCCACTT AGCCGGGATG TGGTTATTGA AGTTGAGGAC 
ACAAAACCAT TGCTTCCAAC CAAGTTCTCG GTATCTCCAT CCTCTTGCCA TTTGTGTGGC 
TCTCTGTCCT GCTGTTGCAC ACGTGTCCAT GATCAGTTAA CTGAAAAGGA GGAAGAAGTT 
GTTGACTGGT GGAGCAAATT TTATGCATCT GTTGGAGAAC CTGAAAAGTG TGGGCAATAT 
ATCCAGAAGG AATATGCCAA ACTAAAGGTA TATAATTGTG AACTAGAGAG TGTTCCAGAG 
TTTAAGGGTC TGACAGACTT TTCAGATACC TTTAAGCTTT ACAGAGGGAA GTCTGATGAG 
ATTGATGATC CTTCAGTGGT AGGAGAGTTC AAGGGCTCCT TTAGAATCTA TCCACTGCCT 
GATGATCCAA ATGTGCCAGC TCCTCCCAGG CAGTTTCGGG AATTACCTGA CAGTGTCCCA 
CAGGAATGCA TTGTGAGGAT TTATATTGTT CGGGGCATAG ATCTCCAGCC TCAGGATAAC 
AATGGCCTGT GTGACCCTTA TATAAAAATA TCCCTGAGTA AAAAAGTCAT TGAAGATCGA 
GATAATTACA TTCCAAATAC ACTCAACCCA ATTTTTGGAA GGATGTATGA GCTAAGCTGC 
TTCTTACCTC AAGAAAAAGA CCTGAAAATT TCTGTCTATG ATTATGACAC ACTTACTCGT 
GATGAAAAAG TAGGAGAAAC TATCATTGAT CTAGAAAATC GCTTCCTTTC CCGATTTGGG 
TCGAATTGTG GCATAGCAGA GCAATATTGC ATTTCTGGAG TCAATACTTG GAGAGATCAA 
CTAAAACCAA CCCAATTGTT ATGCAATGTA GCTAAGTTTA AAGGATTGCC ACTCCCTATT 
CTTTCTGAAG ATGGAAATAG GCTAAGATTT GGAGGTCGAG AGTACACCTT GGAAGAATTT 
GAGGCCAACA GAAATCTGCA CCAGCACCTG GGACCTGCAG AGGAGCGTCT TGCTCTGTAT 
ATCCTTCGGA CTCAGGGTCA AGTCCCTGAG CATGTGGAAA CAAGGACTTT GTACAGCACC 
TTCCAACCAA ATATTTCACA GGGAAAACTT CAGATGTGGG TGGATGTTTT CCCCAAAAGT 
TTGGGGCCAC CAGGCCCTCC TTTCAATATC ACTCCCCGGA AGGCCAAGAG GTATGTCCTA 
CGTGTGATCA TTTGGAACAC TAAAGATGTC ATTCTTGATG AGAAAAGTAT CACAGGAGAA 
GAGATGACTG ACATCTATGT GAAAGGCTGG ATTCCTGGGA ATGAAGAAAA CAAGCAGAAG 
ACAGATGTCC ATTACAGGTC ATTGGATGGT GAAGGAAACT TTAATTGGAG ATTTGTTTTC 
CCTTTTGAAT ACCTGCCTGC TGAACAACTC TGTATTGTTT CTAAAAAAGA ACATTTCTGG 
AGTATTGACC AGACTGAATT TCGAGTTCCT CCCAGACTCA TCATTCAGAT ATGGGACAAT 
GATAAGTTTT CTTTAGATGA CTACTTGGGT TTTCTGGAAC TTGATTTACA CAACACAATT 
ATCCCTGCAA AGTCTTCAGA AAAGTGCAAA TTGGACATGA TTTCAGAGTA TAGGGCAGTG 
AATCCTCTAA AGTCCAAGAC AGCCTCACTT TTTGAACAGA AATCGATGAA AGGATGGTGG 
CCATGCTATG CAGAAAAAGA TGGCGCACGT GTGATGACTG GTAAAGTGGA GATGACTTTG 
GAAGTCCTCA ATGAGAAGGA GGTTGATGAA AGACCAGCTG GGAAAGGAAG GGATGAGCCC 
AACATGAACC CCAAGCTAGA ATTACCAAAT CGACCAGAAA CTTCATTCCT TTGGTTTACC 
AACCCATGCA AGACAATGAA ATTCATCGTG TGGCGTCGAT TTAAATGGGT CATCATTGGC 
TTACTCATCC TGCTGGTCTT GTTGCTGTTT TTGGCAGTAC TCCTATATTC TCTGCCGAAC 
TATATATCAA TGAAGATCGT GAGACCAAAT GCAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016281647.1
CDS	180..6398
Translation	MLRVIVESASDIPKGKFGKPDPIVSVIFKDEKKKTKKIDNELNPVWNEIFEFDLKGIPLDFSSTLGITVKDFETIGQNKLIGVATISLKDLIGGQSRSLPYKLISLVNEKGQDTGATIDLVVAYDPPAAMATNPNELSGITIEGTGEEEEEDTGDEDTAFKVIAPRVPATNISEAQLARRITKGKNTRKILSNKPQDFQIRVRVIEGRQLSGNNIKPVVKVHVCGQTHRTRIKRGNNPFFDELFFYNVHMTPLELVDETISIRVYDSHSLRADCLMGEFKIDIGYIYDEPGHAVMRKWLLLNDPEDISSGAKGYMKVSMFVLGTGDEPPTEKRDRDNDSDDVESNLLLPAGIAMRWVTFLLKIYRAEDIPQMDDAFSQTVKEIFGANVDKKNLVDPFVEVCFAGKKVSTGIIEKNANPEWNQVVNLQIKFPSMCEKIKLTVYDWDRLTKNDVVGTTYLYLSKIAASGGEVEEKTGETEVGFVPTFGPCYLNLYGSPREYTGFPDPYDDLNSGKGEGVAYRGRILVELNTLLEKTPPDKKVETISNDDLLVVEKYQRRRKYSLSAVFHSATMLQDIGEAIQFEVSIGNYGNKFDATCKPLASTTQYSRAVFDGNFYYYLPWAQKKPVVTLTSYWEDISHRLDAVNTILVMAERLHVNIENLKSGIQAKMSGNHLAEMWLKLIDELIEDTSMPLPSLEGKSNVTILDTQIQKLRLRSLIQIQEAARKMRSEAKHVKSTLTEIEDWFDKLMQLSEEPQNSMPDIIIWMIRGEKRLAYARIPAHQVLYSTTSEEASGKYCGKTQTIFLKYPQDRSYGVKVPAELRVNIWLGLSAVEKKFNSFAEGTFTVFAEMYENQALMFGKWGTSGLVGRHKFSDVTGKIKLKKEFFLPPKGWEWEGEWMVDPERSLLTEADAGHTHFTDEVYQNESRYPGGEWKAMEDSYTDVNGEKAASPNEMTCPPGWEWEDDAWMYDINRAVDEKGWEYGITIPPDNKPKSWVAAEKMYHTHRRRRMVRKRKKDVTQSAASTARGWEALEDKEGWEYASLIGWKFHWKQRSSDTFRRRRWRRKMAPSETHGSAAIFKLEGAIGADTTEDGDEKSTEKNKHSATTVFGANTPIVSCNFDRVYNYHLRCYIYQARNLMALDKDSFSDPYAHVSFLHRSQTTEIIHSTLNPTWDQTLIFDDIEIFGEPQTIIQNPPKVVIELFDNDQVGKDEFLGRSFCSPVVKLNPEVDITPKLLWQPVINGDKACGDVLVAAELILKDKVGSNLPILPSQRAPNLYMVPQGIRPVVQLTAIEILAWGLRNMKNYQMASVTSPSLIIECGGERIESALIKNLKKTPNFPSSVLFMKVLLPKEELYTPPLVIKVIDHRQFGRKPIVGQCTIDLLEQFRCDPYAFKEDIAPQVKASILSAPLSRDVVIEVEDTKPLLPTKFSVSPSSCHLCGSLSCCCTRVHDQLTEKEEEVVDWWSKFYASVGEPEKCGQYIQKEYAKLKVYNCELESVPEFKGLTDFSDTFKLYRGKSDEIDDPSVVGEFKGSFRIYPLPDDPNVPAPPRQFRELPDSVPQECIVRIYIVRGIDLQPQDNNGLCDPYIKISLSKKVIEDRDNYIPNTLNPIFGRMYELSCFLPQEKDLKISVYDYDTLTRDEKVGETIIDLENRFLSRFGSNCGIAEQYCISGVNTWRDQLKPTQLLCNVAKFKGLPLPILSEDGNRLRFGGREYTLEEFEANRNLHQHLGPAEERLALYILRTQGQVPEHVETRTLYSTFQPNISQGKLQMWVDVFPKSLGPPGPPFNITPRKAKRYVLRVIIWNTKDVILDEKSITGEEMTDIYVKGWIPGNEENKQKTDVHYRSLDGEGNFNWRFVFPFEYLPAEQLCIVSKKEHFWSIDQTEFRVPPRLIIQIWDNDKFSLDDYLGFLELDLHNTIIPAKSSEKCKLDMISEYRAVNPLKSKTASLFEQKSMKGWWPCYAEKDGARVMTGKVEMTLEVLNEKEVDERPAGKGRDEPNMNPKLELPNRPETSFLWFTNPCKTMKFIVWRRFKWVIIGLLILLVLLLFLAVLLYSLPNYISMKIVRPNAK

Target ORF information:

RefSeq Version	XM_016426161.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica myoferlin (MYOF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016426161.1

ORF Insert Sequence:

ATGCTTCGGG TCATTGTGGA GTCTGCGAGT GATATCCCTA AAGGGAAATT TGGAAAACCG 
GATCCAATTG TCTCAGTTAT TTTTAAGGAT GAGAAGAAGA AAACGAAGAA AATTGATAAT 
GAACTCAACC CTGTCTGGAA TGAGATATTT GAATTTGATT TGAAGGGCAT ACCATTGGAC 
TTTTCATCTA CTCTTGGCAT CACTGTGAAA GATTTCGAGA CAATTGGACA AAATAAACTG 
ATTGGCGTTG CAACAATATC ACTGAAGGAC TTGATAGGTG GCCAGAGTCG ATCTCTTCCT 
TATAAACTCA TATCTTTGGT AAATGAAAAG GGTCAAGATA CTGGGGCTAC AATCGATCTG 
GTGGTTGCCT ATGATCCACC AGCAGCCATG GCAACAAATC CAAATGAACT AAGTGGGATC 
ACCATAGAAG GCACGGGAGA AGAAGAGGAA GAAGACACAG GAGATGAAGA TACTGCATTC 
AAAGTCATTG CCCCAAGGGT TCCAGCAACA AATATTTCTG AAGCCCAGCT GGCCAGGAGG 
ATTACAAAAG GAAAGAATAC CCGAAAGATC CTTTCCAATA AGCCACAAGA CTTCCAGATT 
CGTGTTAGAG TGATTGAAGG GCGCCAATTA AGTGGGAATA ACATCAAACC AGTGGTCAAA 
GTCCATGTCT GTGGTCAGAC ACATCGAACA AGAATAAAAA GAGGGAATAA TCCATTTTTT 
GACGAATTAT TTTTCTACAA TGTCCACATG ACCCCTCTTG AACTGGTTGA TGAGACAATA 
AGCATTCGGG TATATGATTC CCATTCTTTG AGAGCAGACT GTCTGATGGG AGAATTTAAG 
ATTGATATTG GTTACATTTA TGATGAACCT GGTCATGCTG TCATGAGAAA ATGGCTTCTT 
CTAAATGACC CTGAAGATAT AAGTTCTGGT GCTAAAGGTT ACATGAAAGT CAGCATGTTT 
GTCCTTGGAA CAGGAGATGA ACCACCAACA GAGAAGAGAG ATCGAGATAA TGACAGTGAT 
GATGTGGAGA GTAATTTGTT ACTTCCTGCT GGAATTGCCA TGCGGTGGGT CACTTTCCTC 
CTGAAAATTT ACAGAGCTGA GGATATTCCT CAGATGGATG ATGCCTTTTC ACAGACTGTC 
AAAGAAATAT TTGGAGCCAA TGTAGACAAG AAAAACTTAG TGGATCCATT TGTAGAAGTT 
TGTTTTGCTG GGAAAAAGGT TTCCACAGGC ATAATAGAGA AAAATGCAAA TCCAGAGTGG 
AATCAAGTTG TCAATCTTCA AATCAAGTTT CCTTCAATGT GTGAAAAAAT AAAGTTAACA 
GTATATGACT GGGACCGCCT TACCAAGAAT GATGTAGTAG GGACAACCTA TCTGTATCTT 
TCTAAAATTG CTGCATCGGG TGGGGAAGTT GAAGAGAAAA CAGGAGAAAC AGAAGTGGGC 
TTTGTACCAA CATTTGGACC TTGTTATCTA AATCTTTATG GAAGTCCAAG AGAATACACT 
GGATTCCCAG ATCCATATGA TGACTTGAAT TCTGGAAAGG GTGAAGGAGT TGCCTACAGA 
GGTCGAATCT TGGTAGAACT GAATACTTTA CTTGAGAAAA CACCCCCTGA CAAGAAGGTT 
GAAACCATAT CCAATGATGA TTTGCTGGTT GTTGAGAAAT ATCAGCGTAG GCGAAAATAT 
AGCCTGTCTG CGGTATTTCA TTCTGCAACC ATGCTGCAAG ATATTGGAGA GGCCATTCAG 
TTTGAAGTCA GCATTGGAAA TTACGGCAAT AAGTTTGATG CTACCTGTAA GCCTTTGGCA 
TCGACAACTC AATACAGTCG GGCTGTGTTT GATGGAAATT TCTATTATTA TTTGCCATGG 
GCCCAGAAGA AGCCAGTGGT GACTCTAACT TCTTATTGGG AAGACATTAG TCACAGACTG 
GATGCAGTGA ACACCATTCT TGTTATGGCA GAACGGCTGC ACGTGAACAT TGAGAATTTA 
AAATCAGGAA TACAAGCCAA AATGTCTGGG AATCATTTGG CTGAAATGTG GCTGAAGTTG 
ATTGATGAAC TTATCGAAGA TACAAGCATG CCATTGCCTT CACTAGAAGG GAAATCTAAT 
GTCACCATTC TAGACACTCA GATCCAAAAG CTGCGTCTTC GATCCCTTAT CCAGATTCAA 
GAAGCAGCTA GAAAAATGAG GTCTGAAGCC AAACATGTGA AGTCTACTTT GACAGAAATT 
GAAGATTGGT TTGATAAGCT AATGCAACTT TCTGAAGAGC CACAAAACAG TATGCCCGAT 
ATTATCATAT GGATGATCCG AGGAGAGAAA AGATTGGCCT ATGCTAGAAT TCCTGCACAC 
CAGGTTTTAT ATTCTACTAC CAGTGAAGAA GCTTCTGGAA AGTACTGTGG GAAGACCCAG 
ACCATCTTTT TGAAGTACCC ACAGGACAGG AGCTATGGTG TGAAGGTTCC AGCAGAATTG 
CGGGTTAACA TTTGGCTTGG ATTAAGTGCT GTTGAAAAGA AGTTTAACAG CTTTGCTGAA 
GGAACATTCA CTGTCTTTGC AGAAATGTAT GAAAATCAAG CTTTGATGTT TGGAAAATGG 
GGCACTTCTG GACTTGTGGG TCGACATAAA TTTTCAGATG TCACAGGCAA GATAAAGCTC 
AAGAAAGAGT TCTTTTTGCC CCCTAAAGGC TGGGAATGGG AAGGTGAATG GATGGTGGAT 
CCTGAAAGAA GTTTATTGAC TGAAGCGGAT GCTGGCCACA CACACTTTAC AGATGAAGTG 
TATCAGAATG AAAGCCGCTA TCCAGGTGGA GAATGGAAGG CAATGGAGGA CAGCTACACA 
GATGTGAATG GAGAAAAAGC AGCATCTCCT AATGAGATGA CTTGCCCCCC TGGCTGGGAA 
TGGGAAGATG ATGCTTGGAT GTATGATATA AACCGAGCTG TAGATGAAAA AGGCTGGGAA 
TATGGAATTA CTATTCCTCC TGATAACAAG CCAAAGTCCT GGGTTGCTGC AGAGAAGATG 
TATCATACAC ACAGGCGGCG GCGAATGGTC CGCAAACGCA AAAAGGATGT GACACAGTCG 
GCTGCAAGTA CTGCAAGGGG GTGGGAAGCA CTAGAAGATA AAGAAGGCTG GGAATATGCA 
TCTTTAATTG GATGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACTTT TCGTCGAAGA 
CGCTGGAGAA GAAAGATGGC TCCTTCTGAA ACACATGGTT CAGCTGCTAT CTTTAAACTT 
GAAGGTGCCA TTGGAGCAGA CACTACTGAA GATGGAGATG AAAAGAGCAC GGAGAAAAAC 
AAACATAGTG CAACAACCGT GTTTGGTGCA AACACTCCAA TTGTTTCCTG TAATTTTGAC 
AGAGTATATA ATTATCATCT TCGCTGCTAT ATATATCAAG CTCGAAATCT TATGGCTTTA 
GATAAAGATA GTTTTTCAGA TCCCTATGCT CATGTTTCAT TCCTCCATCG GAGCCAAACA 
ACTGAAATCA TCCACTCAAC ACTGAATCCA ACCTGGGACC AAACTCTAAT ATTTGATGAC 
ATTGAAATTT TTGGAGAACC ACAAACAATC ATACAAAACC CACCCAAAGT TGTCATTGAA 
CTCTTTGACA ATGACCAAGT GGGCAAGGAT GAATTTTTAG GTCGAAGTTT TTGCTCCCCA 
GTTGTCAAGC TAAATCCAGA AGTTGACATC ACCCCTAAGC TTCTCTGGCA ACCAGTCATA 
AATGGAGATA AGGCTTGTGG GGATGTACTT GTAGCTGCTG AGCTTATTCT CAAAGATAAG 
GTTGGCTCCA ATCTTCCAAT TCTCCCATCT CAGAGGGCTC CAAATCTCTA CATGGTTCCT 
CAAGGAATTA GGCCTGTGGT TCAACTCACT GCTATTGAGA TTTTAGCTTG GGGTTTAAGA 
AACATGAAAA ACTACCAGAT GGCTTCTGTG ACTTCCCCAA GTCTTATCAT AGAGTGTGGA 
GGAGAAAGGA TAGAATCAGC ATTGATCAAA AACCTCAAGA AGACACCCAA CTTTCCTAGT 
TCTGTTCTCT TCATGAAAGT GCTATTGCCC AAGGAAGAAT TGTACACACC ACCATTGGTT 
ATCAAAGTGA TCGACCACAG ACAATTTGGG CGAAAGCCCA TCGTGGGTCA GTGCACCATC 
GACCTTCTTG AACAGTTTCG TTGTGACCCT TATGCATTTA AGGAAGATAT TGCACCCCAG 
GTAAAAGCTT CTATCTTGTC TGCCCCACTT AGCCGGGATG TGGTTATTGA AGTTGAGGAC 
ACAAAACCAT TGCTTCCAAC CAAGTTCTCG GTATCTCCAT CCTCTTGCCA TTTGTGTGGC 
TCTCTGTCCT GCTGTTGCAC ACGTGTCCAT GATCAGTTAA CTGAAAAGGA GGAAGAAGTT 
GTTGACTGGT GGAGCAAATT TTATGCATCT GTTGGAGAAC CTGAAAAGTG TGGGCAATAT 
ATCCAGAAGG AATATGCCAA ACTAAAGGTA TATAATTGTG AACTAGAGAG TGTTCCAGAG 
TTTAAGGGTC TGACAGACTT TTCAGATACC TTTAAGCTTT ACAGAGGGAA GTCTGATGAG 
ATTGATGATC CTTCAGTGGT AGGAGAGTTC AAGGGCTCCT TTAGAATCTA TCCACTGCCT 
GATGATCCAA ATGTGCCAGC TCCTCCCAGG CAGTTTCGGG AATTACCTGA CAGTGTCCCA 
CAGGAATGCA TTGTGAGGAT TTATATTGTT CGGGGCATAG ATCTCCAGCC TCAGGATAAC 
AATGGCCTGT GTGACCCTTA TATAAAAATA TCCCTGAGTA AAAAAGTCAT TGAAGATCGA 
GATAATTACA TTCCAAATAC ACTCAACCCA ATTTTTGGAA GGATGTATGA GCTAAGCTGC 
TTCTTACCTC AAGAAAAAGA CCTGAAAATT TCTGTCTATG ATTATGACAC ACTTACTCGT 
GATGAAAAAG TAGGAGAAAC TATCATTGAT CTAGAAAATC GCTTCCTTTC CCGATTTGGG 
TCGAATTGTG GCATAGCAGA GCAATATTGC ATTTCTGGAG TCAATACTTG GAGAGATCAA 
CTAAAACCAA CCCAATTGTT ATGCAATGTA GCTAAGTTTA AAGGATTGCC ACTCCCTATT 
CTTTCTGAAG ATGGAAATAG GCTAAGATTT GGAGGTCGAG AGTACACCTT GGAAGAATTT 
GAGGCCAACA GAAATCTGCA CCAGCACCTG GGACCTGCAG AGGAGCGTCT TGCTCTGTAT 
ATCCTTCGGA CTCAGGGTCA AGTCCCTGAG CATGTGGAAA CAAGGACTTT GTACAGCACC 
TTCCAACCAA ATATTTCACA GGGAAAACTT CAGATGTGGG TGGATGTTTT CCCCAAAAGT 
TTGGGGCCAC CAGGCCCTCC TTTCAATATC ACTCCCCGGA AGGCCAAGAG GTATGTCCTA 
CGTGTGATCA TTTGGAACAC TAAAGATGTC ATTCTTGATG AGAAAAGTAT CACAGGAGAA 
GAGATGACTG ACATCTATGT GAAAGGCTGG ATTCCTGGGA ATGAAGAAAA CAAGCAGAAG 
ACAGATGTCC ATTACAGGTC ATTGGATGGT GAAGGAAACT TTAATTGGAG ATTTGTTTTC 
CCTTTTGAAT ACCTGCCTGC TGAACAACTC TGTATTGTTT CTAAAAAAGA ACATTTCTGG 
AGTATTGACC AGACTGAATT TCGAGTTCCT CCCAGACTCA TCATTCAGAT ATGGGACAAT 
GATAAGTTTT CTTTAGATGA CTACTTGGGT TTTCTGGAAC TTGATTTACA CAACACAATT 
ATCCCTGCAA AGTCTTCAGA AAAGTGCAAA TTGGACATGA TTTCAGAGTA TAGGGCAGTG 
AATCCTCTAA AGTCCAAGAC AGCCTCACTT TTTGAACAGA AATCGATGAA AGGATGGTGG 
CCATGCTATG CAGAAAAAGA TGGCGCACGT GTGATGACTG GTAAAGTGGA GATGACTTTG 
GAAGTCCTCA ATGAGAAGGA GGTTGATGAA AGACCAGCTG GGAAAGGAAG GGATGAGCCC 
AACATGAACC CCAAGCTAGA ATTACCAAAT CGACCAGAAA CTTCATTCCT TTGGTTTACC 
AACCCATGCA AGACAATGAA ATTCATCGTG TGGCGTCGAT TTAAATGGGT CATCATTGGC 
TTACTCATCC TGCTGGTCTT GTTGCTGTTT TTGGCAGTAC TCCTATATTC TCTGCCGAAC 
TATATATCAA TGAAGATCGT GAGACCAAAT GCAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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MYOF cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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