NUMA1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	NUMA1
Entrez Gene ID	100015927
Full Name	nuclear mitotic apparatus protein 1
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007491049.1	XP_007491111.1	nuclear mitotic apparatus protein 1 isoform X2
XM_007491050.1	XP_007491112.1	nuclear mitotic apparatus protein 1 isoform X3
XM_007491051.2	XP_007491113.1	nuclear mitotic apparatus protein 1 isoform X3
XM_007491053.2	XP_007491115.1	nuclear mitotic apparatus protein 1 isoform X3
XM_007491047.2	XP_007491109.1	nuclear mitotic apparatus protein 1 isoform X1
XM_007491046.1	XP_007491108.1	nuclear mitotic apparatus protein 1 isoform X1
XM_007491048.2	XP_007491110.1	nuclear mitotic apparatus protein 1 isoform X1
XM_007491052.1	XP_007491114.1	nuclear mitotic apparatus protein 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following NUMA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NUMA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc22043	XM_007491048.2 Latest version!	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc22043	XM_007491047.2 Latest version!	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc22043	XM_007491046.1 Latest version!	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc22044	XM_007491049.1 Latest version!	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc22045	XM_007491051.2 Latest version!	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc22045	XM_007491052.1 Latest version!	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc22045	XM_007491053.2 Latest version!	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMc22045	XM_007491050.1 Latest version!	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NUMA1 ( XM_007491047.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007491109.1 Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X2, mRNA.

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CloneID	OMc22043
Clone ID Related Accession (Same CDS sequence)	XM_007491047.2 , XM_007491046.1 , XM_007491048.2
Accession Version	XM_007491047.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007491047.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTTG GACTCTTGCC ACAGGGCTCC AAGTGTGCAG TGGGTTCACC ATCTGACATA 
ACCAATATGA CTCTTCATGC GACCAGGACA GCTGCACTCC TCTCTTGGGT GAACAGCCTA 
CAGATTGCTG AACCTGTGAC ATCAGTGCTA GAGCTCCAAG ACTGCAACAT CTTTATCCAG 
ATCATCAACC GCATCCATGG CACTGAAGAG GGGCAGCAGA TCCTGGAGCA GCCTCTGCTG 
GACAGACTCA ACTTCATCGC CAACTTTCTG CAGAAAAACT GCAAACATCG GTCCTCCTCA 
GAGAGCCTAG TGTCTGTGCA GAAGCTAGAA GATGGGGAGG AGTTGGAGTT GGCCAAGGTG 
ATCATGTTAC TCTTATACCA TTCCACCATG AGTTCCAAAA GTTTGAGGGA TTGGAACCAA 
TTTGACTACA AAATCCAGTC GGAGTTGGCT GCCATCCTTA AATTTGTGTT GGACCATGAA 
GATGGGTTCA GTCTGAATGA CAACTTAGAG AGTTTCCTTC AGAAAGCTCC TCTCCTTTCC 
AGTAGTCCTA GCACCAGCTC TGATGAGCTT TCCCCAATGC GTACCCAACA AACCAGGAAG 
GAAGTTCGAT TCTTGGAACT GCAGAAGATT GCCTCCTCAT CAGGTCTAAA CAACTTCTTG 
TCAGGGTCTC CTGCTTCTCC CATGGGTGAT ATCCTTCAGA CCCCCCAGTT CCAAATGAGA 
CGGTTGAAGA AACAGCTGGC AGATGAGCGG GCTAGCCGAG ATGAGTTGGA GCTGGAGTTA 
GCCAAAAGCT GCCAACTACT GGCTGAGAAG GACACACAGA TTTCCACGAT GCAGCAGCGT 
ATTGATCGCT TGGCTCTCCT AAATGAGAAG CAGGCCACAG GACCCCTGGA ATCTAAAGAG 
CTGGAAGAAT TACGTGACAA GAATGAGAGT CTGATTGTAC GGTTTCATGA AGCCTTGAAA 
CAGTGCCAGG ACTTGAAGAC TGAGAAGGGC CAGATGGAGC GCAAGATTGA CCAGCTCTCT 
GAGGAGAATG GGGACCTTTC TTTCAAGCTT CGGGATTTTG CAAGCCATCT GCTGCAGCTA 
CAGGAGGCTC TCAATGATCT GACAGAAGAA CATAATAGCT CCACAAAGGA ATGGAGGGAG 
AAACAGGTTC ACCTGGAGAC AGAACTTGGC ACCACCCTGC AAGACAAGAA ATGCCTTGAA 
GAGAAGAATG AGATCCTCCA GGGAAAAATA TCACAGTTGG AGGAGCAGCT GGCACAGGTT 
GGGAAGAATC CATCTCAAGA GAAGGGAGAG GTGTTGGGCG ACATCTTGCA GCTGGAAGCC 
CTGAAACAAG AAGTAGCCAA CTTTGCTGCA AAGGATGCTG AGCTTCAAGC CACAATAAAG 
CAACTGGAGG ATGAACGGAA TCAGTGGAAA TTAGAGATGC AAGCTGAGCA GGACCGCTTT 
AAGGAAGAGA AGCAGCAGCT GGAGCAGAAG GCTCAGACTC AGGAAGCCCA GCTGACTGCT 
CAGGTGGCCA CCCTCCATGC CACCCTCCAG CAGCAGGACC ATGATCTGGC TGACCTCAAG 
CAGCAGACTG AAAAGGAACA AGCACAACTG ACTGAGACCC TTCAACAACA AGAGCTGGCT 
TCCCAAGGCC TCCAGCGCCA GGTGGAGCAA CTGAGCAACA AGCTAAAGCA AGAGGAGGGA 
AGGCTGGAGG GACTGGTCCA GGATTGGAAG GTGACCAGCC AGACCAAGGA CCAACAGCTG 
GCAGCAGCTA TGGAGAAGTA CCAGGAGGCC CAGCAAGAGA GGGATGCAGC CCTGAAACAG 
TTGAAGGAGT TGGAGAAAAC GAAAGCTGTG GAGTTGGGAG CTCTGCAACA GCAGCTCCAA 
GCTACCAAGG ATGCCTGTAA CAGTGCCCAG TCTTGTGCTG CAAAGGCTGA GCAGGAGAAG 
ACCATACTGA GCCAGAAGGT GGAGGAGCTT AGCACGTGTG TAGTTGAACT GAGGACCTGG 
CAAGAGGAGC ACAAAGCAGA AGCTAAATTG GCAGAAAGCC TTCAGACTCG GGTCGAGGAA 
CTGGAGAACC AGCTGAAGGC TGAACAACAG AAACTAGCAG AGAGAGAAGA ACTAGCTCGG 
GAGAATGCCC AGCTCCAGGA ACGACTTCTT TCCCTGGAAG ATTCGTTGCA GAATACTAAG 
GGCATCCTGG AAGATGAGAA GCGACAAGCT GTAGAGGCCC TGGAGGGGCA ACTACGCCGC 
ATTTCTGAGC TGGAGGCAGA GACGCAGAGC CTTGGGCAGC AGCGAGACCA AGATCGGCGG 
CAGCTGGAGG AGGCAAAGGC TGGGCATCAG GGTCTGGAGG CCCAGCTCCA GAAGCTTGGA 
GAAGAGCATC GTTCCAAAGT AGAAACCTTG CAGCAGGAAC TGGAAAAGGT GGCAGCTGCC 
CGGCAAGAGA CAGAGGGAAA GTGCAAGCAG CTGGCCTCAG AGGCAGCTTC CTGGCGTACT 
CGTTATGAAG AGAACCAGAA GGAGGGGGCG CAGCGTGGTG CCCTGCATGA GGAACAGTTG 
AAGGCCCTGA ATGAGGAGCA AGAGAAAACC CAAAAGCAGC TGCAGGCTGC CCAGGAGAGA 
TTGGCAGTGC TGGAGGCTCT CACCAAACGC CAGGATGCTA AACAGCAAGA ATACACTCGG 
CTTGAGGCTG ACCTGTCAGG TGCCCTCCGG CAGCTCCAGG AGAAGGAAGA GCGGGCCCAG 
GATCTGGCCG ACAGCCTGTG CAAGCTGCGG GAGGAGGTAG ATCAGCTCAC AGCTGCAGTC 
AGGAAAGCAG ATGAGGAGAA GGAAGCCTCT TCTCAGAGGC TGGCTGAAGA GCAGCAACGG 
GTGCAAGAGC TTGAGGCTGC CACAAAGCAG CTGGAAGAGC AGCAGAACCA GAAACTAAGC 
AGGATCCAGG CGGCCCTGCA GGCTAAGGAG CAAGAAGCAG AGCAGCTGAC AGGAAAGCTA 
GAGCAAGCAC GCGGTGCTCT GGCAGAGAGC CGGGGGCACC AGGAGGAAGA GCGTGTCCAG 
TGGGAGCGGG AAAGAGTCCG TGCAGAGGTG GAACGAGACC AGGCCAGAGC CGACCTGGCC 
ACTGAGAAGG CTGCTAAGGC AGAACTGGAG GCAAGGCTGC AGAGCTCCCT GAGTGAGCAG 
CGGGTGGAGT TCAGTGCCCT GCAGGAAGCC TTGGCCCATG CCCTGGAAGG AGCAGAAGGG 
AAGGAACAGC AGCTTGGAGA GCTTCAGAAG CAAGAGGCCG CCCTGCGGGC AGAACTGACT 
AAGCTGCAGA AGACCACTGA GGACTTGAGA GAAAGGTTGG CTCAGGAGGA GGCAGCTGCC 
CAAGCAGATG CTCTGGGGCC TGCACAGTCG GGCGCCCAGG AGTCAGAGGC TCTGCGGGCC 
GAGCTGGGTG AGCTGGAGCA GCGGTGCCGG GAGCAGGAGG AGAAGGCCTC TGAGCTGGAG 
CGCCGCCTTG AGGCTTCTCG GAAGGCCCAG GCCAGTCAGG AAAACACTAC TGCTGCCCTC 
CGGGCTCAGC TGGAGGAAAG CACCCAAGAG CTAGCTGCCC TCCGAGTGGC AGCTCAGGAG 
CACAGCAAGG CTGAAGAGGA GCAAAAAGCC CAGGTGGCCC ATGCCCAGCA GGAGGCAGAG 
AAGAATAGCA GCCTCATCAG CAGCTTGGAG CAGGAGGTGT CCATCTTGAA CCTACAGGTT 
CTGGAGAAAG AAGGAGAAAG CAAAGAGCTG AAGCGTCTAG TGCTGGCTGA GTCAGAGAAA 
AGCCAGAAGC TGGAGGAGCG CCTGCGCCTG CTTCAGACAG AAACTGCCAG TGCCAGCACA 
CGGGCGGCAG AGTGCAGCGC AGCCCTGAGC TCTGAGGTGG AGGCGCTCCG GGAAGAGGCT 
GAAAAGCAGC GAGCGGCTGC AGATGGTCTC CGGAGGGAGC TGGCCTCCCA GGCTGAGCAT 
GCAGAGGGCC TGGGACAGGA ACTGAAGACT TGGCGTGAGA GGCTATCCCA GAAGGAGCAG 
TCCCTGTCCT CACTGCAGCA AGAGCTTGCC ACCTCCAAGG CCCTGGTTGG GGAACTGTTG 
CCCATCAAGC ACCTCTGCCA GCAGTTAGAG GCCGAGAAGG GCACCTTGCA GAGCCGGCAC 
CGAGAGGAGC TTGAGCAGGG TGAGAAGGCT GTGGCGGCGC TGCAGGCAGA GCTTCTTCGG 
GCCCGCCGAG AGCTTGGAGA CCTCACTGCA TTGCGACAGA AAGTGACTGA GCAGGAGCAG 
ATGGCTCAGC AGCTGAGGGC AGAGAATGCC AGCTATGCTG ACCAACTGAG TGCACTACAA 
CAGGCCCATA GCAGGCTAGC AGAGGAGAAC CGAAACCTGG GGGAGCGGGC CAGCCACGGG 
CGGCAGCAGC TGGAGGCTGA GCTTGGACGA GCCCAGGAAC TTGAGGCAGT GCGGGCTGAG 
GCAGAAGCCC AGGCAGTTCG GAGCCGGGAG GAGGCCCTAG ATACAGCCAG GCAGCTGGAG 
ACCATGACGG CCAAGTATGA GAGTGCCAAG AGCAAGGTCT TGGAGGAGAG GCAGAGGTTC 
CAGGAAGAGC GGCAGAAACT CACTGCCCAG GTGGAGCAAC TAGAGGCATT TCAGAGAGAA 
CAGACTAAAC AGGTGGAGGA ACTGAATAAG AAGCTGGCCC AGCAACAAAA TATGAAGCCC 
CCTGAAAGTG AAATACAGCA GCAAGCTCAT CATTACCAGG CCCAACTCAG TGAGCTGCAG 
GCCCAACTGA GCCAGAAGGA ACAGGCTGCT GAGCACTATA AGGTGCAGAT GGAAAGAGCC 
AAGGCTCACT ATGATGCCAA GAAGCAGCAG AACCAGGAGC TGCAGGAACG TCTGCAGAGC 
TTGGAGAATC TACAGAAGGA GAATGCTGAA CTGCGGACCG AGTCTGAGAA GCTCGGCTGG 
GAACTGAAGC AAGCTGGGCT GAAGACCAAG GAAGCTGAGC AAGCCTGCCG CCATCTTACT 
GCCCAGGTTC GAACTCTGGA GGCACAGGTT GCTCATGCAG ATCAGCAGCT CCGAGATCTT 
GGCAAGTTTC AAGTGGCAAC AGATGCACTG AAAACCCGAG AAGTCCGAGG GCTGCCCCAA 
CTGGATCTGA GCACTGACAG CCTAGAATTG AGCAGTGAGG AAGGGACCCC ACTGACCTCT 
ACAAGATCAG CAGCTCACCG TACTCGAAAG TCATCTCGAA CCCAGCCTGA TGGCACCAGC 
CTCCCTGGGG AGCCAGCTTC ACCCATCTCT CAGCGCCTGC CTTCTAAGGT GGAGTCCCTG 
GAGAGCTTGT ACTTCACCCC AATACCTGCC CGAGGACAGG CCCCCTTAGA AAGCAGCATG 
GACTCACTGG GTGACATTGT ATTGGATTCT GGTCCCAAGA CCCGCTCAGC CCGAAGGCGG 
ACCACACAGA TCATTGACAT CACAATGCTG TCCAGGAAAC TGGACACAGA TGAACCGGAC 
AGTTCGAATT CATCCTTCTA CAGCACACAG TCAGCCCCTG CCCCACAGCG GGGTCTCCGT 
TCTGCGTCTT CGGCTCAGTC CCTGGCTAAC TGTCCCTCTC GGGAGTCCCG GACCAGGCTG 
GGCTCCCTGG AAGATGGGAA CTCAGCCTTG CTCAGCCTTC CTGGTTATAG GCCCAACACT 
CGAAGCTCAG CCCGGCACTC CCAGGCCAGA GCATCTAGCG GGGCCCCTTC AGGTCGGAGC 
AGCTTCTATA CAGGCACCTG CCAGGATGAG CCAGAGCAGC TGGATGACTG GAACCGGATT 
GCAGAACTTC AGCAGCGCAA CCGTGTCTGC CTCCCACACC TGAAGACCTC TTACCCCTTG 
GAGGCCAGGC CTTCCATGAG CCTGGCCACC ATCACAGACG AAGAGATGAA GACGGGAGAC 
CCCCAGGAGA CCCTTCGCCG AGCCAGCATG CAGCCAGCCC AGATCCATGA GTTTAGTGGC 
ACACATCGCC TTCCCTCAAG CATCACCACC CGGCAGCACC GGAAGCGCAT CTCCTCGGAG 
CTTGACCAAG GCCTCGGCAC CCCTGAGGCC AAGAAACCGA CCAGCTGTTT TCCTCGTCCC 
CTCACCCCCC GGGACAGACA TGAAGGCCGG AAGATGAGTG CCGCCGAGGG CCAGAAGAAG 
GGGGCCCCTG CCCCCGGCAA GCAGACCAAC AAGCGCCAGT CATTAGCCTT CAACATTCTG 
AACACACCCA AGAGACTGGG GAACAGCCTG CTCCGGGGAG CCACCAGGAA ATCTTCGGCT 
AAGACCTCCC CACTCCCCCG AAGTGGGACC CGCCGCTCTC CCCGAATTGC CACTACTACC 
ATCACCACTG GCAGCACCTC TCCCCGGGCC AGGGGCAAGG CAAAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007491109.1
CDS	166..6456
Translation	MSFGLLPQGSKCAVGSPSDITNMTLHATRTAALLSWVNSLQIAEPVTSVLELQDCNIFIQIINRIHGTEEGQQILEQPLLDRLNFIANFLQKNCKHRSSSESLVSVQKLEDGEELELAKVIMLLLYHSTMSSKSLRDWNQFDYKIQSELAAILKFVLDHEDGFSLNDNLESFLQKAPLLSSSPSTSSDELSPMRTQQTRKEVRFLELQKIASSSGLNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERASRDELELELAKSCQLLAEKDTQISTMQQRIDRLALLNEKQATGPLESKELEELRDKNESLIVRFHEALKQCQDLKTEKGQMERKIDQLSEENGDLSFKLRDFASHLLQLQEALNDLTEEHNSSTKEWREKQVHLETELGTTLQDKKCLEEKNEILQGKISQLEEQLAQVGKNPSQEKGEVLGDILQLEALKQEVANFAAKDAELQATIKQLEDERNQWKLEMQAEQDRFKEEKQQLEQKAQTQEAQLTAQVATLHATLQQQDHDLADLKQQTEKEQAQLTETLQQQELASQGLQRQVEQLSNKLKQEEGRLEGLVQDWKVTSQTKDQQLAAAMEKYQEAQQERDAALKQLKELEKTKAVELGALQQQLQATKDACNSAQSCAAKAEQEKTILSQKVEELSTCVVELRTWQEEHKAEAKLAESLQTRVEELENQLKAEQQKLAEREELARENAQLQERLLSLEDSLQNTKGILEDEKRQAVEALEGQLRRISELEAETQSLGQQRDQDRRQLEEAKAGHQGLEAQLQKLGEEHRSKVETLQQELEKVAAARQETEGKCKQLASEAASWRTRYEENQKEGAQRGALHEEQLKALNEEQEKTQKQLQAAQERLAVLEALTKRQDAKQQEYTRLEADLSGALRQLQEKEERAQDLADSLCKLREEVDQLTAAVRKADEEKEASSQRLAEEQQRVQELEAATKQLEEQQNQKLSRIQAALQAKEQEAEQLTGKLEQARGALAESRGHQEEERVQWERERVRAEVERDQARADLATEKAAKAELEARLQSSLSEQRVEFSALQEALAHALEGAEGKEQQLGELQKQEAALRAELTKLQKTTEDLRERLAQEEAAAQADALGPAQSGAQESEALRAELGELEQRCREQEEKASELERRLEASRKAQASQENTTAALRAQLEESTQELAALRVAAQEHSKAEEEQKAQVAHAQQEAEKNSSLISSLEQEVSILNLQVLEKEGESKELKRLVLAESEKSQKLEERLRLLQTETASASTRAAECSAALSSEVEALREEAEKQRAAADGLRRELASQAEHAEGLGQELKTWRERLSQKEQSLSSLQQELATSKALVGELLPIKHLCQQLEAEKGTLQSRHREELEQGEKAVAALQAELLRARRELGDLTALRQKVTEQEQMAQQLRAENASYADQLSALQQAHSRLAEENRNLGERASHGRQQLEAELGRAQELEAVRAEAEAQAVRSREEALDTARQLETMTAKYESAKSKVLEERQRFQEERQKLTAQVEQLEAFQREQTKQVEELNKKLAQQQNMKPPESEIQQQAHHYQAQLSELQAQLSQKEQAAEHYKVQMERAKAHYDAKKQQNQELQERLQSLENLQKENAELRTESEKLGWELKQAGLKTKEAEQACRHLTAQVRTLEAQVAHADQQLRDLGKFQVATDALKTREVRGLPQLDLSTDSLELSSEEGTPLTSTRSAAHRTRKSSRTQPDGTSLPGEPASPISQRLPSKVESLESLYFTPIPARGQAPLESSMDSLGDIVLDSGPKTRSARRRTTQIIDITMLSRKLDTDEPDSSNSSFYSTQSAPAPQRGLRSASSAQSLANCPSRESRTRLGSLEDGNSALLSLPGYRPNTRSSARHSQARASSGAPSGRSSFYTGTCQDEPEQLDDWNRIAELQQRNRVCLPHLKTSYPLEARPSMSLATITDEEMKTGDPQETLRRASMQPAQIHEFSGTHRLPSSITTRQHRKRISSELDQGLGTPEAKKPTSCFPRPLTPRDRHEGRKMSAAEGQKKGAPAPGKQTNKRQSLAFNILNTPKRLGNSLLRGATRKSSAKTSPLPRSGTRRSPRIATTTITTGSTSPRARGKAKH

Target ORF information:

RefSeq Version	XM_007491047.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007491047.2

ORF Insert Sequence:

ATGAGCTTTG GACTCTTGCC ACAGGGCTCC AAGTGTGCAG TGGGTTCACC ATCTGACATA 
ACCAATATGA CTCTTCATGC GACCAGGACA GCTGCACTCC TCTCTTGGGT GAACAGCCTA 
CAGATTGCTG AACCTGTGAC ATCAGTGCTA GAGCTCCAAG ACTGCAACAT CTTTATCCAG 
ATCATCAACC GCATCCATGG CACTGAAGAG GGGCAGCAGA TCCTGGAGCA GCCTCTGCTG 
GACAGACTCA ACTTCATCGC CAACTTTCTG CAGAAAAACT GCAAACATCG GTCCTCCTCA 
GAGAGCCTAG TGTCTGTGCA GAAGCTAGAA GATGGGGAGG AGTTGGAGTT GGCCAAGGTG 
ATCATGTTAC TCTTATACCA TTCCACCATG AGTTCCAAAA GTTTGAGGGA TTGGAACCAA 
TTTGACTACA AAATCCAGTC GGAGTTGGCT GCCATCCTTA AATTTGTGTT GGACCATGAA 
GATGGGTTCA GTCTGAATGA CAACTTAGAG AGTTTCCTTC AGAAAGCTCC TCTCCTTTCC 
AGTAGTCCTA GCACCAGCTC TGATGAGCTT TCCCCAATGC GTACCCAACA AACCAGGAAG 
GAAGTTCGAT TCTTGGAACT GCAGAAGATT GCCTCCTCAT CAGGTCTAAA CAACTTCTTG 
TCAGGGTCTC CTGCTTCTCC CATGGGTGAT ATCCTTCAGA CCCCCCAGTT CCAAATGAGA 
CGGTTGAAGA AACAGCTGGC AGATGAGCGG GCTAGCCGAG ATGAGTTGGA GCTGGAGTTA 
GCCAAAAGCT GCCAACTACT GGCTGAGAAG GACACACAGA TTTCCACGAT GCAGCAGCGT 
ATTGATCGCT TGGCTCTCCT AAATGAGAAG CAGGCCACAG GACCCCTGGA ATCTAAAGAG 
CTGGAAGAAT TACGTGACAA GAATGAGAGT CTGATTGTAC GGTTTCATGA AGCCTTGAAA 
CAGTGCCAGG ACTTGAAGAC TGAGAAGGGC CAGATGGAGC GCAAGATTGA CCAGCTCTCT 
GAGGAGAATG GGGACCTTTC TTTCAAGCTT CGGGATTTTG CAAGCCATCT GCTGCAGCTA 
CAGGAGGCTC TCAATGATCT GACAGAAGAA CATAATAGCT CCACAAAGGA ATGGAGGGAG 
AAACAGGTTC ACCTGGAGAC AGAACTTGGC ACCACCCTGC AAGACAAGAA ATGCCTTGAA 
GAGAAGAATG AGATCCTCCA GGGAAAAATA TCACAGTTGG AGGAGCAGCT GGCACAGGTT 
GGGAAGAATC CATCTCAAGA GAAGGGAGAG GTGTTGGGCG ACATCTTGCA GCTGGAAGCC 
CTGAAACAAG AAGTAGCCAA CTTTGCTGCA AAGGATGCTG AGCTTCAAGC CACAATAAAG 
CAACTGGAGG ATGAACGGAA TCAGTGGAAA TTAGAGATGC AAGCTGAGCA GGACCGCTTT 
AAGGAAGAGA AGCAGCAGCT GGAGCAGAAG GCTCAGACTC AGGAAGCCCA GCTGACTGCT 
CAGGTGGCCA CCCTCCATGC CACCCTCCAG CAGCAGGACC ATGATCTGGC TGACCTCAAG 
CAGCAGACTG AAAAGGAACA AGCACAACTG ACTGAGACCC TTCAACAACA AGAGCTGGCT 
TCCCAAGGCC TCCAGCGCCA GGTGGAGCAA CTGAGCAACA AGCTAAAGCA AGAGGAGGGA 
AGGCTGGAGG GACTGGTCCA GGATTGGAAG GTGACCAGCC AGACCAAGGA CCAACAGCTG 
GCAGCAGCTA TGGAGAAGTA CCAGGAGGCC CAGCAAGAGA GGGATGCAGC CCTGAAACAG 
TTGAAGGAGT TGGAGAAAAC GAAAGCTGTG GAGTTGGGAG CTCTGCAACA GCAGCTCCAA 
GCTACCAAGG ATGCCTGTAA CAGTGCCCAG TCTTGTGCTG CAAAGGCTGA GCAGGAGAAG 
ACCATACTGA GCCAGAAGGT GGAGGAGCTT AGCACGTGTG TAGTTGAACT GAGGACCTGG 
CAAGAGGAGC ACAAAGCAGA AGCTAAATTG GCAGAAAGCC TTCAGACTCG GGTCGAGGAA 
CTGGAGAACC AGCTGAAGGC TGAACAACAG AAACTAGCAG AGAGAGAAGA ACTAGCTCGG 
GAGAATGCCC AGCTCCAGGA ACGACTTCTT TCCCTGGAAG ATTCGTTGCA GAATACTAAG 
GGCATCCTGG AAGATGAGAA GCGACAAGCT GTAGAGGCCC TGGAGGGGCA ACTACGCCGC 
ATTTCTGAGC TGGAGGCAGA GACGCAGAGC CTTGGGCAGC AGCGAGACCA AGATCGGCGG 
CAGCTGGAGG AGGCAAAGGC TGGGCATCAG GGTCTGGAGG CCCAGCTCCA GAAGCTTGGA 
GAAGAGCATC GTTCCAAAGT AGAAACCTTG CAGCAGGAAC TGGAAAAGGT GGCAGCTGCC 
CGGCAAGAGA CAGAGGGAAA GTGCAAGCAG CTGGCCTCAG AGGCAGCTTC CTGGCGTACT 
CGTTATGAAG AGAACCAGAA GGAGGGGGCG CAGCGTGGTG CCCTGCATGA GGAACAGTTG 
AAGGCCCTGA ATGAGGAGCA AGAGAAAACC CAAAAGCAGC TGCAGGCTGC CCAGGAGAGA 
TTGGCAGTGC TGGAGGCTCT CACCAAACGC CAGGATGCTA AACAGCAAGA ATACACTCGG 
CTTGAGGCTG ACCTGTCAGG TGCCCTCCGG CAGCTCCAGG AGAAGGAAGA GCGGGCCCAG 
GATCTGGCCG ACAGCCTGTG CAAGCTGCGG GAGGAGGTAG ATCAGCTCAC AGCTGCAGTC 
AGGAAAGCAG ATGAGGAGAA GGAAGCCTCT TCTCAGAGGC TGGCTGAAGA GCAGCAACGG 
GTGCAAGAGC TTGAGGCTGC CACAAAGCAG CTGGAAGAGC AGCAGAACCA GAAACTAAGC 
AGGATCCAGG CGGCCCTGCA GGCTAAGGAG CAAGAAGCAG AGCAGCTGAC AGGAAAGCTA 
GAGCAAGCAC GCGGTGCTCT GGCAGAGAGC CGGGGGCACC AGGAGGAAGA GCGTGTCCAG 
TGGGAGCGGG AAAGAGTCCG TGCAGAGGTG GAACGAGACC AGGCCAGAGC CGACCTGGCC 
ACTGAGAAGG CTGCTAAGGC AGAACTGGAG GCAAGGCTGC AGAGCTCCCT GAGTGAGCAG 
CGGGTGGAGT TCAGTGCCCT GCAGGAAGCC TTGGCCCATG CCCTGGAAGG AGCAGAAGGG 
AAGGAACAGC AGCTTGGAGA GCTTCAGAAG CAAGAGGCCG CCCTGCGGGC AGAACTGACT 
AAGCTGCAGA AGACCACTGA GGACTTGAGA GAAAGGTTGG CTCAGGAGGA GGCAGCTGCC 
CAAGCAGATG CTCTGGGGCC TGCACAGTCG GGCGCCCAGG AGTCAGAGGC TCTGCGGGCC 
GAGCTGGGTG AGCTGGAGCA GCGGTGCCGG GAGCAGGAGG AGAAGGCCTC TGAGCTGGAG 
CGCCGCCTTG AGGCTTCTCG GAAGGCCCAG GCCAGTCAGG AAAACACTAC TGCTGCCCTC 
CGGGCTCAGC TGGAGGAAAG CACCCAAGAG CTAGCTGCCC TCCGAGTGGC AGCTCAGGAG 
CACAGCAAGG CTGAAGAGGA GCAAAAAGCC CAGGTGGCCC ATGCCCAGCA GGAGGCAGAG 
AAGAATAGCA GCCTCATCAG CAGCTTGGAG CAGGAGGTGT CCATCTTGAA CCTACAGGTT 
CTGGAGAAAG AAGGAGAAAG CAAAGAGCTG AAGCGTCTAG TGCTGGCTGA GTCAGAGAAA 
AGCCAGAAGC TGGAGGAGCG CCTGCGCCTG CTTCAGACAG AAACTGCCAG TGCCAGCACA 
CGGGCGGCAG AGTGCAGCGC AGCCCTGAGC TCTGAGGTGG AGGCGCTCCG GGAAGAGGCT 
GAAAAGCAGC GAGCGGCTGC AGATGGTCTC CGGAGGGAGC TGGCCTCCCA GGCTGAGCAT 
GCAGAGGGCC TGGGACAGGA ACTGAAGACT TGGCGTGAGA GGCTATCCCA GAAGGAGCAG 
TCCCTGTCCT CACTGCAGCA AGAGCTTGCC ACCTCCAAGG CCCTGGTTGG GGAACTGTTG 
CCCATCAAGC ACCTCTGCCA GCAGTTAGAG GCCGAGAAGG GCACCTTGCA GAGCCGGCAC 
CGAGAGGAGC TTGAGCAGGG TGAGAAGGCT GTGGCGGCGC TGCAGGCAGA GCTTCTTCGG 
GCCCGCCGAG AGCTTGGAGA CCTCACTGCA TTGCGACAGA AAGTGACTGA GCAGGAGCAG 
ATGGCTCAGC AGCTGAGGGC AGAGAATGCC AGCTATGCTG ACCAACTGAG TGCACTACAA 
CAGGCCCATA GCAGGCTAGC AGAGGAGAAC CGAAACCTGG GGGAGCGGGC CAGCCACGGG 
CGGCAGCAGC TGGAGGCTGA GCTTGGACGA GCCCAGGAAC TTGAGGCAGT GCGGGCTGAG 
GCAGAAGCCC AGGCAGTTCG GAGCCGGGAG GAGGCCCTAG ATACAGCCAG GCAGCTGGAG 
ACCATGACGG CCAAGTATGA GAGTGCCAAG AGCAAGGTCT TGGAGGAGAG GCAGAGGTTC 
CAGGAAGAGC GGCAGAAACT CACTGCCCAG GTGGAGCAAC TAGAGGCATT TCAGAGAGAA 
CAGACTAAAC AGGTGGAGGA ACTGAATAAG AAGCTGGCCC AGCAACAAAA TATGAAGCCC 
CCTGAAAGTG AAATACAGCA GCAAGCTCAT CATTACCAGG CCCAACTCAG TGAGCTGCAG 
GCCCAACTGA GCCAGAAGGA ACAGGCTGCT GAGCACTATA AGGTGCAGAT GGAAAGAGCC 
AAGGCTCACT ATGATGCCAA GAAGCAGCAG AACCAGGAGC TGCAGGAACG TCTGCAGAGC 
TTGGAGAATC TACAGAAGGA GAATGCTGAA CTGCGGACCG AGTCTGAGAA GCTCGGCTGG 
GAACTGAAGC AAGCTGGGCT GAAGACCAAG GAAGCTGAGC AAGCCTGCCG CCATCTTACT 
GCCCAGGTTC GAACTCTGGA GGCACAGGTT GCTCATGCAG ATCAGCAGCT CCGAGATCTT 
GGCAAGTTTC AAGTGGCAAC AGATGCACTG AAAACCCGAG AAGTCCGAGG GCTGCCCCAA 
CTGGATCTGA GCACTGACAG CCTAGAATTG AGCAGTGAGG AAGGGACCCC ACTGACCTCT 
ACAAGATCAG CAGCTCACCG TACTCGAAAG TCATCTCGAA CCCAGCCTGA TGGCACCAGC 
CTCCCTGGGG AGCCAGCTTC ACCCATCTCT CAGCGCCTGC CTTCTAAGGT GGAGTCCCTG 
GAGAGCTTGT ACTTCACCCC AATACCTGCC CGAGGACAGG CCCCCTTAGA AAGCAGCATG 
GACTCACTGG GTGACATTGT ATTGGATTCT GGTCCCAAGA CCCGCTCAGC CCGAAGGCGG 
ACCACACAGA TCATTGACAT CACAATGCTG TCCAGGAAAC TGGACACAGA TGAACCGGAC 
AGTTCGAATT CATCCTTCTA CAGCACACAG TCAGCCCCTG CCCCACAGCG GGGTCTCCGT 
TCTGCGTCTT CGGCTCAGTC CCTGGCTAAC TGTCCCTCTC GGGAGTCCCG GACCAGGCTG 
GGCTCCCTGG AAGATGGGAA CTCAGCCTTG CTCAGCCTTC CTGGTTATAG GCCCAACACT 
CGAAGCTCAG CCCGGCACTC CCAGGCCAGA GCATCTAGCG GGGCCCCTTC AGGTCGGAGC 
AGCTTCTATA CAGGCACCTG CCAGGATGAG CCAGAGCAGC TGGATGACTG GAACCGGATT 
GCAGAACTTC AGCAGCGCAA CCGTGTCTGC CTCCCACACC TGAAGACCTC TTACCCCTTG 
GAGGCCAGGC CTTCCATGAG CCTGGCCACC ATCACAGACG AAGAGATGAA GACGGGAGAC 
CCCCAGGAGA CCCTTCGCCG AGCCAGCATG CAGCCAGCCC AGATCCATGA GTTTAGTGGC 
ACACATCGCC TTCCCTCAAG CATCACCACC CGGCAGCACC GGAAGCGCAT CTCCTCGGAG 
CTTGACCAAG GCCTCGGCAC CCCTGAGGCC AAGAAACCGA CCAGCTGTTT TCCTCGTCCC 
CTCACCCCCC GGGACAGACA TGAAGGCCGG AAGATGAGTG CCGCCGAGGG CCAGAAGAAG 
GGGGCCCCTG CCCCCGGCAA GCAGACCAAC AAGCGCCAGT CATTAGCCTT CAACATTCTG 
AACACACCCA AGAGACTGGG GAACAGCCTG CTCCGGGGAG CCACCAGGAA ATCTTCGGCT 
AAGACCTCCC CACTCCCCCG AAGTGGGACC CGCCGCTCTC CCCGAATTGC CACTACTACC 
ATCACCACTG GCAGCACCTC TCCCCGGGCC AGGGGCAAGG CAAAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_007491046.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007491108.1 Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.

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CloneID	OMc22043
Clone ID Related Accession (Same CDS sequence)	XM_007491047.2 , XM_007491046.1 , XM_007491048.2
Accession Version	XM_007491046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTTG GACTCTTGCC ACAGGGCTCC AAGTGTGCAG TGGGTTCACC ATCTGACATA 
ACCAATATGA CTCTTCATGC GACCAGGACA GCTGCACTCC TCTCTTGGGT GAACAGCCTA 
CAGATTGCTG AACCTGTGAC ATCAGTGCTA GAGCTCCAAG ACTGCAACAT CTTTATCCAG 
ATCATCAACC GCATCCATGG CACTGAAGAG GGGCAGCAGA TCCTGGAGCA GCCTCTGCTG 
GACAGACTCA ACTTCATCGC CAACTTTCTG CAGAAAAACT GCAAACATCG GTCCTCCTCA 
GAGAGCCTAG TGTCTGTGCA GAAGCTAGAA GATGGGGAGG AGTTGGAGTT GGCCAAGGTG 
ATCATGTTAC TCTTATACCA TTCCACCATG AGTTCCAAAA GTTTGAGGGA TTGGAACCAA 
TTTGACTACA AAATCCAGTC GGAGTTGGCT GCCATCCTTA AATTTGTGTT GGACCATGAA 
GATGGGTTCA GTCTGAATGA CAACTTAGAG AGTTTCCTTC AGAAAGCTCC TCTCCTTTCC 
AGTAGTCCTA GCACCAGCTC TGATGAGCTT TCCCCAATGC GTACCCAACA AACCAGGAAG 
GAAGTTCGAT TCTTGGAACT GCAGAAGATT GCCTCCTCAT CAGGTCTAAA CAACTTCTTG 
TCAGGGTCTC CTGCTTCTCC CATGGGTGAT ATCCTTCAGA CCCCCCAGTT CCAAATGAGA 
CGGTTGAAGA AACAGCTGGC AGATGAGCGG GCTAGCCGAG ATGAGTTGGA GCTGGAGTTA 
GCCAAAAGCT GCCAACTACT GGCTGAGAAG GACACACAGA TTTCCACGAT GCAGCAGCGT 
ATTGATCGCT TGGCTCTCCT AAATGAGAAG CAGGCCACAG GACCCCTGGA ATCTAAAGAG 
CTGGAAGAAT TACGTGACAA GAATGAGAGT CTGATTGTAC GGTTTCATGA AGCCTTGAAA 
CAGTGCCAGG ACTTGAAGAC TGAGAAGGGC CAGATGGAGC GCAAGATTGA CCAGCTCTCT 
GAGGAGAATG GGGACCTTTC TTTCAAGCTT CGGGATTTTG CAAGCCATCT GCTGCAGCTA 
CAGGAGGCTC TCAATGATCT GACAGAAGAA CATAATAGCT CCACAAAGGA ATGGAGGGAG 
AAACAGGTTC ACCTGGAGAC AGAACTTGGC ACCACCCTGC AAGACAAGAA ATGCCTTGAA 
GAGAAGAATG AGATCCTCCA GGGAAAAATA TCACAGTTGG AGGAGCAGCT GGCACAGGTT 
GGGAAGAATC CATCTCAAGA GAAGGGAGAG GTGTTGGGCG ACATCTTGCA GCTGGAAGCC 
CTGAAACAAG AAGTAGCCAA CTTTGCTGCA AAGGATGCTG AGCTTCAAGC CACAATAAAG 
CAACTGGAGG ATGAACGGAA TCAGTGGAAA TTAGAGATGC AAGCTGAGCA GGACCGCTTT 
AAGGAAGAGA AGCAGCAGCT GGAGCAGAAG GCTCAGACTC AGGAAGCCCA GCTGACTGCT 
CAGGTGGCCA CCCTCCATGC CACCCTCCAG CAGCAGGACC ATGATCTGGC TGACCTCAAG 
CAGCAGACTG AAAAGGAACA AGCACAACTG ACTGAGACCC TTCAACAACA AGAGCTGGCT 
TCCCAAGGCC TCCAGCGCCA GGTGGAGCAA CTGAGCAACA AGCTAAAGCA AGAGGAGGGA 
AGGCTGGAGG GACTGGTCCA GGATTGGAAG GTGACCAGCC AGACCAAGGA CCAACAGCTG 
GCAGCAGCTA TGGAGAAGTA CCAGGAGGCC CAGCAAGAGA GGGATGCAGC CCTGAAACAG 
TTGAAGGAGT TGGAGAAAAC GAAAGCTGTG GAGTTGGGAG CTCTGCAACA GCAGCTCCAA 
GCTACCAAGG ATGCCTGTAA CAGTGCCCAG TCTTGTGCTG CAAAGGCTGA GCAGGAGAAG 
ACCATACTGA GCCAGAAGGT GGAGGAGCTT AGCACGTGTG TAGTTGAACT GAGGACCTGG 
CAAGAGGAGC ACAAAGCAGA AGCTAAATTG GCAGAAAGCC TTCAGACTCG GGTCGAGGAA 
CTGGAGAACC AGCTGAAGGC TGAACAACAG AAACTAGCAG AGAGAGAAGA ACTAGCTCGG 
GAGAATGCCC AGCTCCAGGA ACGACTTCTT TCCCTGGAAG ATTCGTTGCA GAATACTAAG 
GGCATCCTGG AAGATGAGAA GCGACAAGCT GTAGAGGCCC TGGAGGGGCA ACTACGCCGC 
ATTTCTGAGC TGGAGGCAGA GACGCAGAGC CTTGGGCAGC AGCGAGACCA AGATCGGCGG 
CAGCTGGAGG AGGCAAAGGC TGGGCATCAG GGTCTGGAGG CCCAGCTCCA GAAGCTTGGA 
GAAGAGCATC GTTCCAAAGT AGAAACCTTG CAGCAGGAAC TGGAAAAGGT GGCAGCTGCC 
CGGCAAGAGA CAGAGGGAAA GTGCAAGCAG CTGGCCTCAG AGGCAGCTTC CTGGCGTACT 
CGTTATGAAG AGAACCAGAA GGAGGGGGCG CAGCGTGGTG CCCTGCATGA GGAACAGTTG 
AAGGCCCTGA ATGAGGAGCA AGAGAAAACC CAAAAGCAGC TGCAGGCTGC CCAGGAGAGA 
TTGGCAGTGC TGGAGGCTCT CACCAAACGC CAGGATGCTA AACAGCAAGA ATACACTCGG 
CTTGAGGCTG ACCTGTCAGG TGCCCTCCGG CAGCTCCAGG AGAAGGAAGA GCGGGCCCAG 
GATCTGGCCG ACAGCCTGTG CAAGCTGCGG GAGGAGGTAG ATCAGCTCAC AGCTGCAGTC 
AGGAAAGCAG ATGAGGAGAA GGAAGCCTCT TCTCAGAGGC TGGCTGAAGA GCAGCAACGG 
GTGCAAGAGC TTGAGGCTGC CACAAAGCAG CTGGAAGAGC AGCAGAACCA GAAACTAAGC 
AGGATCCAGG CGGCCCTGCA GGCTAAGGAG CAAGAAGCAG AGCAGCTGAC AGGAAAGCTA 
GAGCAAGCAC GCGGTGCTCT GGCAGAGAGC CGGGGGCACC AGGAGGAAGA GCGTGTCCAG 
TGGGAGCGGG AAAGAGTCCG TGCAGAGGTG GAACGAGACC AGGCCAGAGC CGACCTGGCC 
ACTGAGAAGG CTGCTAAGGC AGAACTGGAG GCAAGGCTGC AGAGCTCCCT GAGTGAGCAG 
CGGGTGGAGT TCAGTGCCCT GCAGGAAGCC TTGGCCCATG CCCTGGAAGG AGCAGAAGGG 
AAGGAACAGC AGCTTGGAGA GCTTCAGAAG CAAGAGGCCG CCCTGCGGGC AGAACTGACT 
AAGCTGCAGA AGACCACTGA GGACTTGAGA GAAAGGTTGG CTCAGGAGGA GGCAGCTGCC 
CAAGCAGATG CTCTGGGGCC TGCACAGTCG GGCGCCCAGG AGTCAGAGGC TCTGCGGGCC 
GAGCTGGGTG AGCTGGAGCA GCGGTGCCGG GAGCAGGAGG AGAAGGCCTC TGAGCTGGAG 
CGCCGCCTTG AGGCTTCTCG GAAGGCCCAG GCCAGTCAGG AAAACACTAC TGCTGCCCTC 
CGGGCTCAGC TGGAGGAAAG CACCCAAGAG CTAGCTGCCC TCCGAGTGGC AGCTCAGGAG 
CACAGCAAGG CTGAAGAGGA GCAAAAAGCC CAGGTGGCCC ATGCCCAGCA GGAGGCAGAG 
AAGAATAGCA GCCTCATCAG CAGCTTGGAG CAGGAGGTGT CCATCTTGAA CCTACAGGTT 
CTGGAGAAAG AAGGAGAAAG CAAAGAGCTG AAGCGTCTAG TGCTGGCTGA GTCAGAGAAA 
AGCCAGAAGC TGGAGGAGCG CCTGCGCCTG CTTCAGACAG AAACTGCCAG TGCCAGCACA 
CGGGCGGCAG AGTGCAGCGC AGCCCTGAGC TCTGAGGTGG AGGCGCTCCG GGAAGAGGCT 
GAAAAGCAGC GAGCGGCTGC AGATGGTCTC CGGAGGGAGC TGGCCTCCCA GGCTGAGCAT 
GCAGAGGGCC TGGGACAGGA ACTGAAGACT TGGCGTGAGA GGCTATCCCA GAAGGAGCAG 
TCCCTGTCCT CACTGCAGCA AGAGCTTGCC ACCTCCAAGG CCCTGGTTGG GGAACTGTTG 
CCCATCAAGC ACCTCTGCCA GCAGTTAGAG GCCGAGAAGG GCACCTTGCA GAGCCGGCAC 
CGAGAGGAGC TTGAGCAGGG TGAGAAGGCT GTGGCGGCGC TGCAGGCAGA GCTTCTTCGG 
GCCCGCCGAG AGCTTGGAGA CCTCACTGCA TTGCGACAGA AAGTGACTGA GCAGGAGCAG 
ATGGCTCAGC AGCTGAGGGC AGAGAATGCC AGCTATGCTG ACCAACTGAG TGCACTACAA 
CAGGCCCATA GCAGGCTAGC AGAGGAGAAC CGAAACCTGG GGGAGCGGGC CAGCCACGGG 
CGGCAGCAGC TGGAGGCTGA GCTTGGACGA GCCCAGGAAC TTGAGGCAGT GCGGGCTGAG 
GCAGAAGCCC AGGCAGTTCG GAGCCGGGAG GAGGCCCTAG ATACAGCCAG GCAGCTGGAG 
ACCATGACGG CCAAGTATGA GAGTGCCAAG AGCAAGGTCT TGGAGGAGAG GCAGAGGTTC 
CAGGAAGAGC GGCAGAAACT CACTGCCCAG GTGGAGCAAC TAGAGGCATT TCAGAGAGAA 
CAGACTAAAC AGGTGGAGGA ACTGAATAAG AAGCTGGCCC AGCAACAAAA TATGAAGCCC 
CCTGAAAGTG AAATACAGCA GCAAGCTCAT CATTACCAGG CCCAACTCAG TGAGCTGCAG 
GCCCAACTGA GCCAGAAGGA ACAGGCTGCT GAGCACTATA AGGTGCAGAT GGAAAGAGCC 
AAGGCTCACT ATGATGCCAA GAAGCAGCAG AACCAGGAGC TGCAGGAACG TCTGCAGAGC 
TTGGAGAATC TACAGAAGGA GAATGCTGAA CTGCGGACCG AGTCTGAGAA GCTCGGCTGG 
GAACTGAAGC AAGCTGGGCT GAAGACCAAG GAAGCTGAGC AAGCCTGCCG CCATCTTACT 
GCCCAGGTTC GAACTCTGGA GGCACAGGTT GCTCATGCAG ATCAGCAGCT CCGAGATCTT 
GGCAAGTTTC AAGTGGCAAC AGATGCACTG AAAACCCGAG AAGTCCGAGG GCTGCCCCAA 
CTGGATCTGA GCACTGACAG CCTAGAATTG AGCAGTGAGG AAGGGACCCC ACTGACCTCT 
ACAAGATCAG CAGCTCACCG TACTCGAAAG TCATCTCGAA CCCAGCCTGA TGGCACCAGC 
CTCCCTGGGG AGCCAGCTTC ACCCATCTCT CAGCGCCTGC CTTCTAAGGT GGAGTCCCTG 
GAGAGCTTGT ACTTCACCCC AATACCTGCC CGAGGACAGG CCCCCTTAGA AAGCAGCATG 
GACTCACTGG GTGACATTGT ATTGGATTCT GGTCCCAAGA CCCGCTCAGC CCGAAGGCGG 
ACCACACAGA TCATTGACAT CACAATGCTG TCCAGGAAAC TGGACACAGA TGAACCGGAC 
AGTTCGAATT CATCCTTCTA CAGCACACAG TCAGCCCCTG CCCCACAGCG GGGTCTCCGT 
TCTGCGTCTT CGGCTCAGTC CCTGGCTAAC TGTCCCTCTC GGGAGTCCCG GACCAGGCTG 
GGCTCCCTGG AAGATGGGAA CTCAGCCTTG CTCAGCCTTC CTGGTTATAG GCCCAACACT 
CGAAGCTCAG CCCGGCACTC CCAGGCCAGA GCATCTAGCG GGGCCCCTTC AGGTCGGAGC 
AGCTTCTATA CAGGCACCTG CCAGGATGAG CCAGAGCAGC TGGATGACTG GAACCGGATT 
GCAGAACTTC AGCAGCGCAA CCGTGTCTGC CTCCCACACC TGAAGACCTC TTACCCCTTG 
GAGGCCAGGC CTTCCATGAG CCTGGCCACC ATCACAGACG AAGAGATGAA GACGGGAGAC 
CCCCAGGAGA CCCTTCGCCG AGCCAGCATG CAGCCAGCCC AGATCCATGA GTTTAGTGGC 
ACACATCGCC TTCCCTCAAG CATCACCACC CGGCAGCACC GGAAGCGCAT CTCCTCGGAG 
CTTGACCAAG GCCTCGGCAC CCCTGAGGCC AAGAAACCGA CCAGCTGTTT TCCTCGTCCC 
CTCACCCCCC GGGACAGACA TGAAGGCCGG AAGATGAGTG CCGCCGAGGG CCAGAAGAAG 
GGGGCCCCTG CCCCCGGCAA GCAGACCAAC AAGCGCCAGT CATTAGCCTT CAACATTCTG 
AACACACCCA AGAGACTGGG GAACAGCCTG CTCCGGGGAG CCACCAGGAA ATCTTCGGCT 
AAGACCTCCC CACTCCCCCG AAGTGGGACC CGCCGCTCTC CCCGAATTGC CACTACTACC 
ATCACCACTG GCAGCACCTC TCCCCGGGCC AGGGGCAAGG CAAAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007491108.1
CDS	299..6589
Translation	MSFGLLPQGSKCAVGSPSDITNMTLHATRTAALLSWVNSLQIAEPVTSVLELQDCNIFIQIINRIHGTEEGQQILEQPLLDRLNFIANFLQKNCKHRSSSESLVSVQKLEDGEELELAKVIMLLLYHSTMSSKSLRDWNQFDYKIQSELAAILKFVLDHEDGFSLNDNLESFLQKAPLLSSSPSTSSDELSPMRTQQTRKEVRFLELQKIASSSGLNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERASRDELELELAKSCQLLAEKDTQISTMQQRIDRLALLNEKQATGPLESKELEELRDKNESLIVRFHEALKQCQDLKTEKGQMERKIDQLSEENGDLSFKLRDFASHLLQLQEALNDLTEEHNSSTKEWREKQVHLETELGTTLQDKKCLEEKNEILQGKISQLEEQLAQVGKNPSQEKGEVLGDILQLEALKQEVANFAAKDAELQATIKQLEDERNQWKLEMQAEQDRFKEEKQQLEQKAQTQEAQLTAQVATLHATLQQQDHDLADLKQQTEKEQAQLTETLQQQELASQGLQRQVEQLSNKLKQEEGRLEGLVQDWKVTSQTKDQQLAAAMEKYQEAQQERDAALKQLKELEKTKAVELGALQQQLQATKDACNSAQSCAAKAEQEKTILSQKVEELSTCVVELRTWQEEHKAEAKLAESLQTRVEELENQLKAEQQKLAEREELARENAQLQERLLSLEDSLQNTKGILEDEKRQAVEALEGQLRRISELEAETQSLGQQRDQDRRQLEEAKAGHQGLEAQLQKLGEEHRSKVETLQQELEKVAAARQETEGKCKQLASEAASWRTRYEENQKEGAQRGALHEEQLKALNEEQEKTQKQLQAAQERLAVLEALTKRQDAKQQEYTRLEADLSGALRQLQEKEERAQDLADSLCKLREEVDQLTAAVRKADEEKEASSQRLAEEQQRVQELEAATKQLEEQQNQKLSRIQAALQAKEQEAEQLTGKLEQARGALAESRGHQEEERVQWERERVRAEVERDQARADLATEKAAKAELEARLQSSLSEQRVEFSALQEALAHALEGAEGKEQQLGELQKQEAALRAELTKLQKTTEDLRERLAQEEAAAQADALGPAQSGAQESEALRAELGELEQRCREQEEKASELERRLEASRKAQASQENTTAALRAQLEESTQELAALRVAAQEHSKAEEEQKAQVAHAQQEAEKNSSLISSLEQEVSILNLQVLEKEGESKELKRLVLAESEKSQKLEERLRLLQTETASASTRAAECSAALSSEVEALREEAEKQRAAADGLRRELASQAEHAEGLGQELKTWRERLSQKEQSLSSLQQELATSKALVGELLPIKHLCQQLEAEKGTLQSRHREELEQGEKAVAALQAELLRARRELGDLTALRQKVTEQEQMAQQLRAENASYADQLSALQQAHSRLAEENRNLGERASHGRQQLEAELGRAQELEAVRAEAEAQAVRSREEALDTARQLETMTAKYESAKSKVLEERQRFQEERQKLTAQVEQLEAFQREQTKQVEELNKKLAQQQNMKPPESEIQQQAHHYQAQLSELQAQLSQKEQAAEHYKVQMERAKAHYDAKKQQNQELQERLQSLENLQKENAELRTESEKLGWELKQAGLKTKEAEQACRHLTAQVRTLEAQVAHADQQLRDLGKFQVATDALKTREVRGLPQLDLSTDSLELSSEEGTPLTSTRSAAHRTRKSSRTQPDGTSLPGEPASPISQRLPSKVESLESLYFTPIPARGQAPLESSMDSLGDIVLDSGPKTRSARRRTTQIIDITMLSRKLDTDEPDSSNSSFYSTQSAPAPQRGLRSASSAQSLANCPSRESRTRLGSLEDGNSALLSLPGYRPNTRSSARHSQARASSGAPSGRSSFYTGTCQDEPEQLDDWNRIAELQQRNRVCLPHLKTSYPLEARPSMSLATITDEEMKTGDPQETLRRASMQPAQIHEFSGTHRLPSSITTRQHRKRISSELDQGLGTPEAKKPTSCFPRPLTPRDRHEGRKMSAAEGQKKGAPAPGKQTNKRQSLAFNILNTPKRLGNSLLRGATRKSSAKTSPLPRSGTRRSPRIATTTITTGSTSPRARGKAKH

Target ORF information:

RefSeq Version	XM_007491046.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007491046.1

ORF Insert Sequence:

ATGAGCTTTG GACTCTTGCC ACAGGGCTCC AAGTGTGCAG TGGGTTCACC ATCTGACATA 
ACCAATATGA CTCTTCATGC GACCAGGACA GCTGCACTCC TCTCTTGGGT GAACAGCCTA 
CAGATTGCTG AACCTGTGAC ATCAGTGCTA GAGCTCCAAG ACTGCAACAT CTTTATCCAG 
ATCATCAACC GCATCCATGG CACTGAAGAG GGGCAGCAGA TCCTGGAGCA GCCTCTGCTG 
GACAGACTCA ACTTCATCGC CAACTTTCTG CAGAAAAACT GCAAACATCG GTCCTCCTCA 
GAGAGCCTAG TGTCTGTGCA GAAGCTAGAA GATGGGGAGG AGTTGGAGTT GGCCAAGGTG 
ATCATGTTAC TCTTATACCA TTCCACCATG AGTTCCAAAA GTTTGAGGGA TTGGAACCAA 
TTTGACTACA AAATCCAGTC GGAGTTGGCT GCCATCCTTA AATTTGTGTT GGACCATGAA 
GATGGGTTCA GTCTGAATGA CAACTTAGAG AGTTTCCTTC AGAAAGCTCC TCTCCTTTCC 
AGTAGTCCTA GCACCAGCTC TGATGAGCTT TCCCCAATGC GTACCCAACA AACCAGGAAG 
GAAGTTCGAT TCTTGGAACT GCAGAAGATT GCCTCCTCAT CAGGTCTAAA CAACTTCTTG 
TCAGGGTCTC CTGCTTCTCC CATGGGTGAT ATCCTTCAGA CCCCCCAGTT CCAAATGAGA 
CGGTTGAAGA AACAGCTGGC AGATGAGCGG GCTAGCCGAG ATGAGTTGGA GCTGGAGTTA 
GCCAAAAGCT GCCAACTACT GGCTGAGAAG GACACACAGA TTTCCACGAT GCAGCAGCGT 
ATTGATCGCT TGGCTCTCCT AAATGAGAAG CAGGCCACAG GACCCCTGGA ATCTAAAGAG 
CTGGAAGAAT TACGTGACAA GAATGAGAGT CTGATTGTAC GGTTTCATGA AGCCTTGAAA 
CAGTGCCAGG ACTTGAAGAC TGAGAAGGGC CAGATGGAGC GCAAGATTGA CCAGCTCTCT 
GAGGAGAATG GGGACCTTTC TTTCAAGCTT CGGGATTTTG CAAGCCATCT GCTGCAGCTA 
CAGGAGGCTC TCAATGATCT GACAGAAGAA CATAATAGCT CCACAAAGGA ATGGAGGGAG 
AAACAGGTTC ACCTGGAGAC AGAACTTGGC ACCACCCTGC AAGACAAGAA ATGCCTTGAA 
GAGAAGAATG AGATCCTCCA GGGAAAAATA TCACAGTTGG AGGAGCAGCT GGCACAGGTT 
GGGAAGAATC CATCTCAAGA GAAGGGAGAG GTGTTGGGCG ACATCTTGCA GCTGGAAGCC 
CTGAAACAAG AAGTAGCCAA CTTTGCTGCA AAGGATGCTG AGCTTCAAGC CACAATAAAG 
CAACTGGAGG ATGAACGGAA TCAGTGGAAA TTAGAGATGC AAGCTGAGCA GGACCGCTTT 
AAGGAAGAGA AGCAGCAGCT GGAGCAGAAG GCTCAGACTC AGGAAGCCCA GCTGACTGCT 
CAGGTGGCCA CCCTCCATGC CACCCTCCAG CAGCAGGACC ATGATCTGGC TGACCTCAAG 
CAGCAGACTG AAAAGGAACA AGCACAACTG ACTGAGACCC TTCAACAACA AGAGCTGGCT 
TCCCAAGGCC TCCAGCGCCA GGTGGAGCAA CTGAGCAACA AGCTAAAGCA AGAGGAGGGA 
AGGCTGGAGG GACTGGTCCA GGATTGGAAG GTGACCAGCC AGACCAAGGA CCAACAGCTG 
GCAGCAGCTA TGGAGAAGTA CCAGGAGGCC CAGCAAGAGA GGGATGCAGC CCTGAAACAG 
TTGAAGGAGT TGGAGAAAAC GAAAGCTGTG GAGTTGGGAG CTCTGCAACA GCAGCTCCAA 
GCTACCAAGG ATGCCTGTAA CAGTGCCCAG TCTTGTGCTG CAAAGGCTGA GCAGGAGAAG 
ACCATACTGA GCCAGAAGGT GGAGGAGCTT AGCACGTGTG TAGTTGAACT GAGGACCTGG 
CAAGAGGAGC ACAAAGCAGA AGCTAAATTG GCAGAAAGCC TTCAGACTCG GGTCGAGGAA 
CTGGAGAACC AGCTGAAGGC TGAACAACAG AAACTAGCAG AGAGAGAAGA ACTAGCTCGG 
GAGAATGCCC AGCTCCAGGA ACGACTTCTT TCCCTGGAAG ATTCGTTGCA GAATACTAAG 
GGCATCCTGG AAGATGAGAA GCGACAAGCT GTAGAGGCCC TGGAGGGGCA ACTACGCCGC 
ATTTCTGAGC TGGAGGCAGA GACGCAGAGC CTTGGGCAGC AGCGAGACCA AGATCGGCGG 
CAGCTGGAGG AGGCAAAGGC TGGGCATCAG GGTCTGGAGG CCCAGCTCCA GAAGCTTGGA 
GAAGAGCATC GTTCCAAAGT AGAAACCTTG CAGCAGGAAC TGGAAAAGGT GGCAGCTGCC 
CGGCAAGAGA CAGAGGGAAA GTGCAAGCAG CTGGCCTCAG AGGCAGCTTC CTGGCGTACT 
CGTTATGAAG AGAACCAGAA GGAGGGGGCG CAGCGTGGTG CCCTGCATGA GGAACAGTTG 
AAGGCCCTGA ATGAGGAGCA AGAGAAAACC CAAAAGCAGC TGCAGGCTGC CCAGGAGAGA 
TTGGCAGTGC TGGAGGCTCT CACCAAACGC CAGGATGCTA AACAGCAAGA ATACACTCGG 
CTTGAGGCTG ACCTGTCAGG TGCCCTCCGG CAGCTCCAGG AGAAGGAAGA GCGGGCCCAG 
GATCTGGCCG ACAGCCTGTG CAAGCTGCGG GAGGAGGTAG ATCAGCTCAC AGCTGCAGTC 
AGGAAAGCAG ATGAGGAGAA GGAAGCCTCT TCTCAGAGGC TGGCTGAAGA GCAGCAACGG 
GTGCAAGAGC TTGAGGCTGC CACAAAGCAG CTGGAAGAGC AGCAGAACCA GAAACTAAGC 
AGGATCCAGG CGGCCCTGCA GGCTAAGGAG CAAGAAGCAG AGCAGCTGAC AGGAAAGCTA 
GAGCAAGCAC GCGGTGCTCT GGCAGAGAGC CGGGGGCACC AGGAGGAAGA GCGTGTCCAG 
TGGGAGCGGG AAAGAGTCCG TGCAGAGGTG GAACGAGACC AGGCCAGAGC CGACCTGGCC 
ACTGAGAAGG CTGCTAAGGC AGAACTGGAG GCAAGGCTGC AGAGCTCCCT GAGTGAGCAG 
CGGGTGGAGT TCAGTGCCCT GCAGGAAGCC TTGGCCCATG CCCTGGAAGG AGCAGAAGGG 
AAGGAACAGC AGCTTGGAGA GCTTCAGAAG CAAGAGGCCG CCCTGCGGGC AGAACTGACT 
AAGCTGCAGA AGACCACTGA GGACTTGAGA GAAAGGTTGG CTCAGGAGGA GGCAGCTGCC 
CAAGCAGATG CTCTGGGGCC TGCACAGTCG GGCGCCCAGG AGTCAGAGGC TCTGCGGGCC 
GAGCTGGGTG AGCTGGAGCA GCGGTGCCGG GAGCAGGAGG AGAAGGCCTC TGAGCTGGAG 
CGCCGCCTTG AGGCTTCTCG GAAGGCCCAG GCCAGTCAGG AAAACACTAC TGCTGCCCTC 
CGGGCTCAGC TGGAGGAAAG CACCCAAGAG CTAGCTGCCC TCCGAGTGGC AGCTCAGGAG 
CACAGCAAGG CTGAAGAGGA GCAAAAAGCC CAGGTGGCCC ATGCCCAGCA GGAGGCAGAG 
AAGAATAGCA GCCTCATCAG CAGCTTGGAG CAGGAGGTGT CCATCTTGAA CCTACAGGTT 
CTGGAGAAAG AAGGAGAAAG CAAAGAGCTG AAGCGTCTAG TGCTGGCTGA GTCAGAGAAA 
AGCCAGAAGC TGGAGGAGCG CCTGCGCCTG CTTCAGACAG AAACTGCCAG TGCCAGCACA 
CGGGCGGCAG AGTGCAGCGC AGCCCTGAGC TCTGAGGTGG AGGCGCTCCG GGAAGAGGCT 
GAAAAGCAGC GAGCGGCTGC AGATGGTCTC CGGAGGGAGC TGGCCTCCCA GGCTGAGCAT 
GCAGAGGGCC TGGGACAGGA ACTGAAGACT TGGCGTGAGA GGCTATCCCA GAAGGAGCAG 
TCCCTGTCCT CACTGCAGCA AGAGCTTGCC ACCTCCAAGG CCCTGGTTGG GGAACTGTTG 
CCCATCAAGC ACCTCTGCCA GCAGTTAGAG GCCGAGAAGG GCACCTTGCA GAGCCGGCAC 
CGAGAGGAGC TTGAGCAGGG TGAGAAGGCT GTGGCGGCGC TGCAGGCAGA GCTTCTTCGG 
GCCCGCCGAG AGCTTGGAGA CCTCACTGCA TTGCGACAGA AAGTGACTGA GCAGGAGCAG 
ATGGCTCAGC AGCTGAGGGC AGAGAATGCC AGCTATGCTG ACCAACTGAG TGCACTACAA 
CAGGCCCATA GCAGGCTAGC AGAGGAGAAC CGAAACCTGG GGGAGCGGGC CAGCCACGGG 
CGGCAGCAGC TGGAGGCTGA GCTTGGACGA GCCCAGGAAC TTGAGGCAGT GCGGGCTGAG 
GCAGAAGCCC AGGCAGTTCG GAGCCGGGAG GAGGCCCTAG ATACAGCCAG GCAGCTGGAG 
ACCATGACGG CCAAGTATGA GAGTGCCAAG AGCAAGGTCT TGGAGGAGAG GCAGAGGTTC 
CAGGAAGAGC GGCAGAAACT CACTGCCCAG GTGGAGCAAC TAGAGGCATT TCAGAGAGAA 
CAGACTAAAC AGGTGGAGGA ACTGAATAAG AAGCTGGCCC AGCAACAAAA TATGAAGCCC 
CCTGAAAGTG AAATACAGCA GCAAGCTCAT CATTACCAGG CCCAACTCAG TGAGCTGCAG 
GCCCAACTGA GCCAGAAGGA ACAGGCTGCT GAGCACTATA AGGTGCAGAT GGAAAGAGCC 
AAGGCTCACT ATGATGCCAA GAAGCAGCAG AACCAGGAGC TGCAGGAACG TCTGCAGAGC 
TTGGAGAATC TACAGAAGGA GAATGCTGAA CTGCGGACCG AGTCTGAGAA GCTCGGCTGG 
GAACTGAAGC AAGCTGGGCT GAAGACCAAG GAAGCTGAGC AAGCCTGCCG CCATCTTACT 
GCCCAGGTTC GAACTCTGGA GGCACAGGTT GCTCATGCAG ATCAGCAGCT CCGAGATCTT 
GGCAAGTTTC AAGTGGCAAC AGATGCACTG AAAACCCGAG AAGTCCGAGG GCTGCCCCAA 
CTGGATCTGA GCACTGACAG CCTAGAATTG AGCAGTGAGG AAGGGACCCC ACTGACCTCT 
ACAAGATCAG CAGCTCACCG TACTCGAAAG TCATCTCGAA CCCAGCCTGA TGGCACCAGC 
CTCCCTGGGG AGCCAGCTTC ACCCATCTCT CAGCGCCTGC CTTCTAAGGT GGAGTCCCTG 
GAGAGCTTGT ACTTCACCCC AATACCTGCC CGAGGACAGG CCCCCTTAGA AAGCAGCATG 
GACTCACTGG GTGACATTGT ATTGGATTCT GGTCCCAAGA CCCGCTCAGC CCGAAGGCGG 
ACCACACAGA TCATTGACAT CACAATGCTG TCCAGGAAAC TGGACACAGA TGAACCGGAC 
AGTTCGAATT CATCCTTCTA CAGCACACAG TCAGCCCCTG CCCCACAGCG GGGTCTCCGT 
TCTGCGTCTT CGGCTCAGTC CCTGGCTAAC TGTCCCTCTC GGGAGTCCCG GACCAGGCTG 
GGCTCCCTGG AAGATGGGAA CTCAGCCTTG CTCAGCCTTC CTGGTTATAG GCCCAACACT 
CGAAGCTCAG CCCGGCACTC CCAGGCCAGA GCATCTAGCG GGGCCCCTTC AGGTCGGAGC 
AGCTTCTATA CAGGCACCTG CCAGGATGAG CCAGAGCAGC TGGATGACTG GAACCGGATT 
GCAGAACTTC AGCAGCGCAA CCGTGTCTGC CTCCCACACC TGAAGACCTC TTACCCCTTG 
GAGGCCAGGC CTTCCATGAG CCTGGCCACC ATCACAGACG AAGAGATGAA GACGGGAGAC 
CCCCAGGAGA CCCTTCGCCG AGCCAGCATG CAGCCAGCCC AGATCCATGA GTTTAGTGGC 
ACACATCGCC TTCCCTCAAG CATCACCACC CGGCAGCACC GGAAGCGCAT CTCCTCGGAG 
CTTGACCAAG GCCTCGGCAC CCCTGAGGCC AAGAAACCGA CCAGCTGTTT TCCTCGTCCC 
CTCACCCCCC GGGACAGACA TGAAGGCCGG AAGATGAGTG CCGCCGAGGG CCAGAAGAAG 
GGGGCCCCTG CCCCCGGCAA GCAGACCAAC AAGCGCCAGT CATTAGCCTT CAACATTCTG 
AACACACCCA AGAGACTGGG GAACAGCCTG CTCCGGGGAG CCACCAGGAA ATCTTCGGCT 
AAGACCTCCC CACTCCCCCG AAGTGGGACC CGCCGCTCTC CCCGAATTGC CACTACTACC 
ATCACCACTG GCAGCACCTC TCCCCGGGCC AGGGGCAAGG CAAAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_007491048.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007491110.1 Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X3, mRNA.

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CloneID	OMc22043
Clone ID Related Accession (Same CDS sequence)	XM_007491047.2 , XM_007491046.1 , XM_007491048.2
Accession Version	XM_007491048.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6291bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	nuclear mitotic apparatus protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007491048.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTTG GACTCTTGCC ACAGGGCTCC AAGTGTGCAG TGGGTTCACC ATCTGACATA 
ACCAATATGA CTCTTCATGC GACCAGGACA GCTGCACTCC TCTCTTGGGT GAACAGCCTA 
CAGATTGCTG AACCTGTGAC ATCAGTGCTA GAGCTCCAAG ACTGCAACAT CTTTATCCAG 
ATCATCAACC GCATCCATGG CACTGAAGAG GGGCAGCAGA TCCTGGAGCA GCCTCTGCTG 
GACAGACTCA ACTTCATCGC CAACTTTCTG CAGAAAAACT GCAAACATCG GTCCTCCTCA 
GAGAGCCTAG TGTCTGTGCA GAAGCTAGAA GATGGGGAGG AGTTGGAGTT GGCCAAGGTG 
ATCATGTTAC TCTTATACCA TTCCACCATG AGTTCCAAAA GTTTGAGGGA TTGGAACCAA 
TTTGACTACA AAATCCAGTC GGAGTTGGCT GCCATCCTTA AATTTGTGTT GGACCATGAA 
GATGGGTTCA GTCTGAATGA CAACTTAGAG AGTTTCCTTC AGAAAGCTCC TCTCCTTTCC 
AGTAGTCCTA GCACCAGCTC TGATGAGCTT TCCCCAATGC GTACCCAACA AACCAGGAAG 
GAAGTTCGAT TCTTGGAACT GCAGAAGATT GCCTCCTCAT CAGGTCTAAA CAACTTCTTG 
TCAGGGTCTC CTGCTTCTCC CATGGGTGAT ATCCTTCAGA CCCCCCAGTT CCAAATGAGA 
CGGTTGAAGA AACAGCTGGC AGATGAGCGG GCTAGCCGAG ATGAGTTGGA GCTGGAGTTA 
GCCAAAAGCT GCCAACTACT GGCTGAGAAG GACACACAGA TTTCCACGAT GCAGCAGCGT 
ATTGATCGCT TGGCTCTCCT AAATGAGAAG CAGGCCACAG GACCCCTGGA ATCTAAAGAG 
CTGGAAGAAT TACGTGACAA GAATGAGAGT CTGATTGTAC GGTTTCATGA AGCCTTGAAA 
CAGTGCCAGG ACTTGAAGAC TGAGAAGGGC CAGATGGAGC GCAAGATTGA CCAGCTCTCT 
GAGGAGAATG GGGACCTTTC TTTCAAGCTT CGGGATTTTG CAAGCCATCT GCTGCAGCTA 
CAGGAGGCTC TCAATGATCT GACAGAAGAA CATAATAGCT CCACAAAGGA ATGGAGGGAG 
AAACAGGTTC ACCTGGAGAC AGAACTTGGC ACCACCCTGC AAGACAAGAA ATGCCTTGAA 
GAGAAGAATG AGATCCTCCA GGGAAAAATA TCACAGTTGG AGGAGCAGCT GGCACAGGTT 
GGGAAGAATC CATCTCAAGA GAAGGGAGAG GTGTTGGGCG ACATCTTGCA GCTGGAAGCC 
CTGAAACAAG AAGTAGCCAA CTTTGCTGCA AAGGATGCTG AGCTTCAAGC CACAATAAAG 
CAACTGGAGG ATGAACGGAA TCAGTGGAAA TTAGAGATGC AAGCTGAGCA GGACCGCTTT 
AAGGAAGAGA AGCAGCAGCT GGAGCAGAAG GCTCAGACTC AGGAAGCCCA GCTGACTGCT 
CAGGTGGCCA CCCTCCATGC CACCCTCCAG CAGCAGGACC ATGATCTGGC TGACCTCAAG 
CAGCAGACTG AAAAGGAACA AGCACAACTG ACTGAGACCC TTCAACAACA AGAGCTGGCT 
TCCCAAGGCC TCCAGCGCCA GGTGGAGCAA CTGAGCAACA AGCTAAAGCA AGAGGAGGGA 
AGGCTGGAGG GACTGGTCCA GGATTGGAAG GTGACCAGCC AGACCAAGGA CCAACAGCTG 
GCAGCAGCTA TGGAGAAGTA CCAGGAGGCC CAGCAAGAGA GGGATGCAGC CCTGAAACAG 
TTGAAGGAGT TGGAGAAAAC GAAAGCTGTG GAGTTGGGAG CTCTGCAACA GCAGCTCCAA 
GCTACCAAGG ATGCCTGTAA CAGTGCCCAG TCTTGTGCTG CAAAGGCTGA GCAGGAGAAG 
ACCATACTGA GCCAGAAGGT GGAGGAGCTT AGCACGTGTG TAGTTGAACT GAGGACCTGG 
CAAGAGGAGC ACAAAGCAGA AGCTAAATTG GCAGAAAGCC TTCAGACTCG GGTCGAGGAA 
CTGGAGAACC AGCTGAAGGC TGAACAACAG AAACTAGCAG AGAGAGAAGA ACTAGCTCGG 
GAGAATGCCC AGCTCCAGGA ACGACTTCTT TCCCTGGAAG ATTCGTTGCA GAATACTAAG 
GGCATCCTGG AAGATGAGAA GCGACAAGCT GTAGAGGCCC TGGAGGGGCA ACTACGCCGC 
ATTTCTGAGC TGGAGGCAGA GACGCAGAGC CTTGGGCAGC AGCGAGACCA AGATCGGCGG 
CAGCTGGAGG AGGCAAAGGC TGGGCATCAG GGTCTGGAGG CCCAGCTCCA GAAGCTTGGA 
GAAGAGCATC GTTCCAAAGT AGAAACCTTG CAGCAGGAAC TGGAAAAGGT GGCAGCTGCC 
CGGCAAGAGA CAGAGGGAAA GTGCAAGCAG CTGGCCTCAG AGGCAGCTTC CTGGCGTACT 
CGTTATGAAG AGAACCAGAA GGAGGGGGCG CAGCGTGGTG CCCTGCATGA GGAACAGTTG 
AAGGCCCTGA ATGAGGAGCA AGAGAAAACC CAAAAGCAGC TGCAGGCTGC CCAGGAGAGA 
TTGGCAGTGC TGGAGGCTCT CACCAAACGC CAGGATGCTA AACAGCAAGA ATACACTCGG 
CTTGAGGCTG ACCTGTCAGG TGCCCTCCGG CAGCTCCAGG AGAAGGAAGA GCGGGCCCAG 
GATCTGGCCG ACAGCCTGTG CAAGCTGCGG GAGGAGGTAG ATCAGCTCAC AGCTGCAGTC 
AGGAAAGCAG ATGAGGAGAA GGAAGCCTCT TCTCAGAGGC TGGCTGAAGA GCAGCAACGG 
GTGCAAGAGC TTGAGGCTGC CACAAAGCAG CTGGAAGAGC AGCAGAACCA GAAACTAAGC 
AGGATCCAGG CGGCCCTGCA GGCTAAGGAG CAAGAAGCAG AGCAGCTGAC AGGAAAGCTA 
GAGCAAGCAC GCGGTGCTCT GGCAGAGAGC CGGGGGCACC AGGAGGAAGA GCGTGTCCAG 
TGGGAGCGGG AAAGAGTCCG TGCAGAGGTG GAACGAGACC AGGCCAGAGC CGACCTGGCC 
ACTGAGAAGG CTGCTAAGGC AGAACTGGAG GCAAGGCTGC AGAGCTCCCT GAGTGAGCAG 
CGGGTGGAGT TCAGTGCCCT GCAGGAAGCC TTGGCCCATG CCCTGGAAGG AGCAGAAGGG 
AAGGAACAGC AGCTTGGAGA GCTTCAGAAG CAAGAGGCCG CCCTGCGGGC AGAACTGACT 
AAGCTGCAGA AGACCACTGA GGACTTGAGA GAAAGGTTGG CTCAGGAGGA GGCAGCTGCC 
CAAGCAGATG CTCTGGGGCC TGCACAGTCG GGCGCCCAGG AGTCAGAGGC TCTGCGGGCC 
GAGCTGGGTG AGCTGGAGCA GCGGTGCCGG GAGCAGGAGG AGAAGGCCTC TGAGCTGGAG 
CGCCGCCTTG AGGCTTCTCG GAAGGCCCAG GCCAGTCAGG AAAACACTAC TGCTGCCCTC 
CGGGCTCAGC TGGAGGAAAG CACCCAAGAG CTAGCTGCCC TCCGAGTGGC AGCTCAGGAG 
CACAGCAAGG CTGAAGAGGA GCAAAAAGCC CAGGTGGCCC ATGCCCAGCA GGAGGCAGAG 
AAGAATAGCA GCCTCATCAG CAGCTTGGAG CAGGAGGTGT CCATCTTGAA CCTACAGGTT 
CTGGAGAAAG AAGGAGAAAG CAAAGAGCTG AAGCGTCTAG TGCTGGCTGA GTCAGAGAAA 
AGCCAGAAGC TGGAGGAGCG CCTGCGCCTG CTTCAGACAG AAACTGCCAG TGCCAGCACA 
CGGGCGGCAG AGTGCAGCGC AGCCCTGAGC TCTGAGGTGG AGGCGCTCCG GGAAGAGGCT 
GAAAAGCAGC GAGCGGCTGC AGATGGTCTC CGGAGGGAGC TGGCCTCCCA GGCTGAGCAT 
GCAGAGGGCC TGGGACAGGA ACTGAAGACT TGGCGTGAGA GGCTATCCCA GAAGGAGCAG 
TCCCTGTCCT CACTGCAGCA AGAGCTTGCC ACCTCCAAGG CCCTGGTTGG GGAACTGTTG 
CCCATCAAGC ACCTCTGCCA GCAGTTAGAG GCCGAGAAGG GCACCTTGCA GAGCCGGCAC 
CGAGAGGAGC TTGAGCAGGG TGAGAAGGCT GTGGCGGCGC TGCAGGCAGA GCTTCTTCGG 
GCCCGCCGAG AGCTTGGAGA CCTCACTGCA TTGCGACAGA AAGTGACTGA GCAGGAGCAG 
ATGGCTCAGC AGCTGAGGGC AGAGAATGCC AGCTATGCTG ACCAACTGAG TGCACTACAA 
CAGGCCCATA GCAGGCTAGC AGAGGAGAAC CGAAACCTGG GGGAGCGGGC CAGCCACGGG 
CGGCAGCAGC TGGAGGCTGA GCTTGGACGA GCCCAGGAAC TTGAGGCAGT GCGGGCTGAG 
GCAGAAGCCC AGGCAGTTCG GAGCCGGGAG GAGGCCCTAG ATACAGCCAG GCAGCTGGAG 
ACCATGACGG CCAAGTATGA GAGTGCCAAG AGCAAGGTCT TGGAGGAGAG GCAGAGGTTC 
CAGGAAGAGC GGCAGAAACT CACTGCCCAG GTGGAGCAAC TAGAGGCATT TCAGAGAGAA 
CAGACTAAAC AGGTGGAGGA ACTGAATAAG AAGCTGGCCC AGCAACAAAA TATGAAGCCC 
CCTGAAAGTG AAATACAGCA GCAAGCTCAT CATTACCAGG CCCAACTCAG TGAGCTGCAG 
GCCCAACTGA GCCAGAAGGA ACAGGCTGCT GAGCACTATA AGGTGCAGAT GGAAAGAGCC 
AAGGCTCACT ATGATGCCAA GAAGCAGCAG AACCAGGAGC TGCAGGAACG TCTGCAGAGC 
TTGGAGAATC TACAGAAGGA GAATGCTGAA CTGCGGACCG AGTCTGAGAA GCTCGGCTGG 
GAACTGAAGC AAGCTGGGCT GAAGACCAAG GAAGCTGAGC AAGCCTGCCG CCATCTTACT 
GCCCAGGTTC GAACTCTGGA GGCACAGGTT GCTCATGCAG ATCAGCAGCT CCGAGATCTT 
GGCAAGTTTC AAGTGGCAAC AGATGCACTG AAAACCCGAG AAGTCCGAGG GCTGCCCCAA 
CTGGATCTGA GCACTGACAG CCTAGAATTG AGCAGTGAGG AAGGGACCCC ACTGACCTCT 
ACAAGATCAG CAGCTCACCG TACTCGAAAG TCATCTCGAA CCCAGCCTGA TGGCACCAGC 
CTCCCTGGGG AGCCAGCTTC ACCCATCTCT CAGCGCCTGC CTTCTAAGGT GGAGTCCCTG 
GAGAGCTTGT ACTTCACCCC AATACCTGCC CGAGGACAGG CCCCCTTAGA AAGCAGCATG 
GACTCACTGG GTGACATTGT ATTGGATTCT GGTCCCAAGA CCCGCTCAGC CCGAAGGCGG 
ACCACACAGA TCATTGACAT CACAATGCTG TCCAGGAAAC TGGACACAGA TGAACCGGAC 
AGTTCGAATT CATCCTTCTA CAGCACACAG TCAGCCCCTG CCCCACAGCG GGGTCTCCGT 
TCTGCGTCTT CGGCTCAGTC CCTGGCTAAC TGTCCCTCTC GGGAGTCCCG GACCAGGCTG 
GGCTCCCTGG AAGATGGGAA CTCAGCCTTG CTCAGCCTTC CTGGTTATAG GCCCAACACT 
CGAAGCTCAG CCCGGCACTC CCAGGCCAGA GCATCTAGCG GGGCCCCTTC AGGTCGGAGC 
AGCTTCTATA CAGGCACCTG CCAGGATGAG CCAGAGCAGC TGGATGACTG GAACCGGATT 
GCAGAACTTC AGCAGCGCAA CCGTGTCTGC CTCCCACACC TGAAGACCTC TTACCCCTTG 
GAGGCCAGGC CTTCCATGAG CCTGGCCACC ATCACAGACG AAGAGATGAA GACGGGAGAC 
CCCCAGGAGA CCCTTCGCCG AGCCAGCATG CAGCCAGCCC AGATCCATGA GTTTAGTGGC 
ACACATCGCC TTCCCTCAAG CATCACCACC CGGCAGCACC GGAAGCGCAT CTCCTCGGAG 
CTTGACCAAG GCCTCGGCAC CCCTGAGGCC AAGAAACCGA CCAGCTGTTT TCCTCGTCCC 
CTCACCCCCC GGGACAGACA TGAAGGCCGG AAGATGAGTG CCGCCGAGGG CCAGAAGAAG 
GGGGCCCCTG CCCCCGGCAA GCAGACCAAC AAGCGCCAGT CATTAGCCTT CAACATTCTG 
AACACACCCA AGAGACTGGG GAACAGCCTG CTCCGGGGAG CCACCAGGAA ATCTTCGGCT 
AAGACCTCCC CACTCCCCCG AAGTGGGACC CGCCGCTCTC CCCGAATTGC CACTACTACC 
ATCACCACTG GCAGCACCTC TCCCCGGGCC AGGGGCAAGG CAAAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007491110.1
CDS	188..6478
Translation	MSFGLLPQGSKCAVGSPSDITNMTLHATRTAALLSWVNSLQIAEPVTSVLELQDCNIFIQIINRIHGTEEGQQILEQPLLDRLNFIANFLQKNCKHRSSSESLVSVQKLEDGEELELAKVIMLLLYHSTMSSKSLRDWNQFDYKIQSELAAILKFVLDHEDGFSLNDNLESFLQKAPLLSSSPSTSSDELSPMRTQQTRKEVRFLELQKIASSSGLNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERASRDELELELAKSCQLLAEKDTQISTMQQRIDRLALLNEKQATGPLESKELEELRDKNESLIVRFHEALKQCQDLKTEKGQMERKIDQLSEENGDLSFKLRDFASHLLQLQEALNDLTEEHNSSTKEWREKQVHLETELGTTLQDKKCLEEKNEILQGKISQLEEQLAQVGKNPSQEKGEVLGDILQLEALKQEVANFAAKDAELQATIKQLEDERNQWKLEMQAEQDRFKEEKQQLEQKAQTQEAQLTAQVATLHATLQQQDHDLADLKQQTEKEQAQLTETLQQQELASQGLQRQVEQLSNKLKQEEGRLEGLVQDWKVTSQTKDQQLAAAMEKYQEAQQERDAALKQLKELEKTKAVELGALQQQLQATKDACNSAQSCAAKAEQEKTILSQKVEELSTCVVELRTWQEEHKAEAKLAESLQTRVEELENQLKAEQQKLAEREELARENAQLQERLLSLEDSLQNTKGILEDEKRQAVEALEGQLRRISELEAETQSLGQQRDQDRRQLEEAKAGHQGLEAQLQKLGEEHRSKVETLQQELEKVAAARQETEGKCKQLASEAASWRTRYEENQKEGAQRGALHEEQLKALNEEQEKTQKQLQAAQERLAVLEALTKRQDAKQQEYTRLEADLSGALRQLQEKEERAQDLADSLCKLREEVDQLTAAVRKADEEKEASSQRLAEEQQRVQELEAATKQLEEQQNQKLSRIQAALQAKEQEAEQLTGKLEQARGALAESRGHQEEERVQWERERVRAEVERDQARADLATEKAAKAELEARLQSSLSEQRVEFSALQEALAHALEGAEGKEQQLGELQKQEAALRAELTKLQKTTEDLRERLAQEEAAAQADALGPAQSGAQESEALRAELGELEQRCREQEEKASELERRLEASRKAQASQENTTAALRAQLEESTQELAALRVAAQEHSKAEEEQKAQVAHAQQEAEKNSSLISSLEQEVSILNLQVLEKEGESKELKRLVLAESEKSQKLEERLRLLQTETASASTRAAECSAALSSEVEALREEAEKQRAAADGLRRELASQAEHAEGLGQELKTWRERLSQKEQSLSSLQQELATSKALVGELLPIKHLCQQLEAEKGTLQSRHREELEQGEKAVAALQAELLRARRELGDLTALRQKVTEQEQMAQQLRAENASYADQLSALQQAHSRLAEENRNLGERASHGRQQLEAELGRAQELEAVRAEAEAQAVRSREEALDTARQLETMTAKYESAKSKVLEERQRFQEERQKLTAQVEQLEAFQREQTKQVEELNKKLAQQQNMKPPESEIQQQAHHYQAQLSELQAQLSQKEQAAEHYKVQMERAKAHYDAKKQQNQELQERLQSLENLQKENAELRTESEKLGWELKQAGLKTKEAEQACRHLTAQVRTLEAQVAHADQQLRDLGKFQVATDALKTREVRGLPQLDLSTDSLELSSEEGTPLTSTRSAAHRTRKSSRTQPDGTSLPGEPASPISQRLPSKVESLESLYFTPIPARGQAPLESSMDSLGDIVLDSGPKTRSARRRTTQIIDITMLSRKLDTDEPDSSNSSFYSTQSAPAPQRGLRSASSAQSLANCPSRESRTRLGSLEDGNSALLSLPGYRPNTRSSARHSQARASSGAPSGRSSFYTGTCQDEPEQLDDWNRIAELQQRNRVCLPHLKTSYPLEARPSMSLATITDEEMKTGDPQETLRRASMQPAQIHEFSGTHRLPSSITTRQHRKRISSELDQGLGTPEAKKPTSCFPRPLTPRDRHEGRKMSAAEGQKKGAPAPGKQTNKRQSLAFNILNTPKRLGNSLLRGATRKSSAKTSPLPRSGTRRSPRIATTTITTGSTSPRARGKAKH

Target ORF information:

RefSeq Version	XM_007491048.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007491048.2

ORF Insert Sequence:

ATGAGCTTTG GACTCTTGCC ACAGGGCTCC AAGTGTGCAG TGGGTTCACC ATCTGACATA 
ACCAATATGA CTCTTCATGC GACCAGGACA GCTGCACTCC TCTCTTGGGT GAACAGCCTA 
CAGATTGCTG AACCTGTGAC ATCAGTGCTA GAGCTCCAAG ACTGCAACAT CTTTATCCAG 
ATCATCAACC GCATCCATGG CACTGAAGAG GGGCAGCAGA TCCTGGAGCA GCCTCTGCTG 
GACAGACTCA ACTTCATCGC CAACTTTCTG CAGAAAAACT GCAAACATCG GTCCTCCTCA 
GAGAGCCTAG TGTCTGTGCA GAAGCTAGAA GATGGGGAGG AGTTGGAGTT GGCCAAGGTG 
ATCATGTTAC TCTTATACCA TTCCACCATG AGTTCCAAAA GTTTGAGGGA TTGGAACCAA 
TTTGACTACA AAATCCAGTC GGAGTTGGCT GCCATCCTTA AATTTGTGTT GGACCATGAA 
GATGGGTTCA GTCTGAATGA CAACTTAGAG AGTTTCCTTC AGAAAGCTCC TCTCCTTTCC 
AGTAGTCCTA GCACCAGCTC TGATGAGCTT TCCCCAATGC GTACCCAACA AACCAGGAAG 
GAAGTTCGAT TCTTGGAACT GCAGAAGATT GCCTCCTCAT CAGGTCTAAA CAACTTCTTG 
TCAGGGTCTC CTGCTTCTCC CATGGGTGAT ATCCTTCAGA CCCCCCAGTT CCAAATGAGA 
CGGTTGAAGA AACAGCTGGC AGATGAGCGG GCTAGCCGAG ATGAGTTGGA GCTGGAGTTA 
GCCAAAAGCT GCCAACTACT GGCTGAGAAG GACACACAGA TTTCCACGAT GCAGCAGCGT 
ATTGATCGCT TGGCTCTCCT AAATGAGAAG CAGGCCACAG GACCCCTGGA ATCTAAAGAG 
CTGGAAGAAT TACGTGACAA GAATGAGAGT CTGATTGTAC GGTTTCATGA AGCCTTGAAA 
CAGTGCCAGG ACTTGAAGAC TGAGAAGGGC CAGATGGAGC GCAAGATTGA CCAGCTCTCT 
GAGGAGAATG GGGACCTTTC TTTCAAGCTT CGGGATTTTG CAAGCCATCT GCTGCAGCTA 
CAGGAGGCTC TCAATGATCT GACAGAAGAA CATAATAGCT CCACAAAGGA ATGGAGGGAG 
AAACAGGTTC ACCTGGAGAC AGAACTTGGC ACCACCCTGC AAGACAAGAA ATGCCTTGAA 
GAGAAGAATG AGATCCTCCA GGGAAAAATA TCACAGTTGG AGGAGCAGCT GGCACAGGTT 
GGGAAGAATC CATCTCAAGA GAAGGGAGAG GTGTTGGGCG ACATCTTGCA GCTGGAAGCC 
CTGAAACAAG AAGTAGCCAA CTTTGCTGCA AAGGATGCTG AGCTTCAAGC CACAATAAAG 
CAACTGGAGG ATGAACGGAA TCAGTGGAAA TTAGAGATGC AAGCTGAGCA GGACCGCTTT 
AAGGAAGAGA AGCAGCAGCT GGAGCAGAAG GCTCAGACTC AGGAAGCCCA GCTGACTGCT 
CAGGTGGCCA CCCTCCATGC CACCCTCCAG CAGCAGGACC ATGATCTGGC TGACCTCAAG 
CAGCAGACTG AAAAGGAACA AGCACAACTG ACTGAGACCC TTCAACAACA AGAGCTGGCT 
TCCCAAGGCC TCCAGCGCCA GGTGGAGCAA CTGAGCAACA AGCTAAAGCA AGAGGAGGGA 
AGGCTGGAGG GACTGGTCCA GGATTGGAAG GTGACCAGCC AGACCAAGGA CCAACAGCTG 
GCAGCAGCTA TGGAGAAGTA CCAGGAGGCC CAGCAAGAGA GGGATGCAGC CCTGAAACAG 
TTGAAGGAGT TGGAGAAAAC GAAAGCTGTG GAGTTGGGAG CTCTGCAACA GCAGCTCCAA 
GCTACCAAGG ATGCCTGTAA CAGTGCCCAG TCTTGTGCTG CAAAGGCTGA GCAGGAGAAG 
ACCATACTGA GCCAGAAGGT GGAGGAGCTT AGCACGTGTG TAGTTGAACT GAGGACCTGG 
CAAGAGGAGC ACAAAGCAGA AGCTAAATTG GCAGAAAGCC TTCAGACTCG GGTCGAGGAA 
CTGGAGAACC AGCTGAAGGC TGAACAACAG AAACTAGCAG AGAGAGAAGA ACTAGCTCGG 
GAGAATGCCC AGCTCCAGGA ACGACTTCTT TCCCTGGAAG ATTCGTTGCA GAATACTAAG 
GGCATCCTGG AAGATGAGAA GCGACAAGCT GTAGAGGCCC TGGAGGGGCA ACTACGCCGC 
ATTTCTGAGC TGGAGGCAGA GACGCAGAGC CTTGGGCAGC AGCGAGACCA AGATCGGCGG 
CAGCTGGAGG AGGCAAAGGC TGGGCATCAG GGTCTGGAGG CCCAGCTCCA GAAGCTTGGA 
GAAGAGCATC GTTCCAAAGT AGAAACCTTG CAGCAGGAAC TGGAAAAGGT GGCAGCTGCC 
CGGCAAGAGA CAGAGGGAAA GTGCAAGCAG CTGGCCTCAG AGGCAGCTTC CTGGCGTACT 
CGTTATGAAG AGAACCAGAA GGAGGGGGCG CAGCGTGGTG CCCTGCATGA GGAACAGTTG 
AAGGCCCTGA ATGAGGAGCA AGAGAAAACC CAAAAGCAGC TGCAGGCTGC CCAGGAGAGA 
TTGGCAGTGC TGGAGGCTCT CACCAAACGC CAGGATGCTA AACAGCAAGA ATACACTCGG 
CTTGAGGCTG ACCTGTCAGG TGCCCTCCGG CAGCTCCAGG AGAAGGAAGA GCGGGCCCAG 
GATCTGGCCG ACAGCCTGTG CAAGCTGCGG GAGGAGGTAG ATCAGCTCAC AGCTGCAGTC 
AGGAAAGCAG ATGAGGAGAA GGAAGCCTCT TCTCAGAGGC TGGCTGAAGA GCAGCAACGG 
GTGCAAGAGC TTGAGGCTGC CACAAAGCAG CTGGAAGAGC AGCAGAACCA GAAACTAAGC 
AGGATCCAGG CGGCCCTGCA GGCTAAGGAG CAAGAAGCAG AGCAGCTGAC AGGAAAGCTA 
GAGCAAGCAC GCGGTGCTCT GGCAGAGAGC CGGGGGCACC AGGAGGAAGA GCGTGTCCAG 
TGGGAGCGGG AAAGAGTCCG TGCAGAGGTG GAACGAGACC AGGCCAGAGC CGACCTGGCC 
ACTGAGAAGG CTGCTAAGGC AGAACTGGAG GCAAGGCTGC AGAGCTCCCT GAGTGAGCAG 
CGGGTGGAGT TCAGTGCCCT GCAGGAAGCC TTGGCCCATG CCCTGGAAGG AGCAGAAGGG 
AAGGAACAGC AGCTTGGAGA GCTTCAGAAG CAAGAGGCCG CCCTGCGGGC AGAACTGACT 
AAGCTGCAGA AGACCACTGA GGACTTGAGA GAAAGGTTGG CTCAGGAGGA GGCAGCTGCC 
CAAGCAGATG CTCTGGGGCC TGCACAGTCG GGCGCCCAGG AGTCAGAGGC TCTGCGGGCC 
GAGCTGGGTG AGCTGGAGCA GCGGTGCCGG GAGCAGGAGG AGAAGGCCTC TGAGCTGGAG 
CGCCGCCTTG AGGCTTCTCG GAAGGCCCAG GCCAGTCAGG AAAACACTAC TGCTGCCCTC 
CGGGCTCAGC TGGAGGAAAG CACCCAAGAG CTAGCTGCCC TCCGAGTGGC AGCTCAGGAG 
CACAGCAAGG CTGAAGAGGA GCAAAAAGCC CAGGTGGCCC ATGCCCAGCA GGAGGCAGAG 
AAGAATAGCA GCCTCATCAG CAGCTTGGAG CAGGAGGTGT CCATCTTGAA CCTACAGGTT 
CTGGAGAAAG AAGGAGAAAG CAAAGAGCTG AAGCGTCTAG TGCTGGCTGA GTCAGAGAAA 
AGCCAGAAGC TGGAGGAGCG CCTGCGCCTG CTTCAGACAG AAACTGCCAG TGCCAGCACA 
CGGGCGGCAG AGTGCAGCGC AGCCCTGAGC TCTGAGGTGG AGGCGCTCCG GGAAGAGGCT 
GAAAAGCAGC GAGCGGCTGC AGATGGTCTC CGGAGGGAGC TGGCCTCCCA GGCTGAGCAT 
GCAGAGGGCC TGGGACAGGA ACTGAAGACT TGGCGTGAGA GGCTATCCCA GAAGGAGCAG 
TCCCTGTCCT CACTGCAGCA AGAGCTTGCC ACCTCCAAGG CCCTGGTTGG GGAACTGTTG 
CCCATCAAGC ACCTCTGCCA GCAGTTAGAG GCCGAGAAGG GCACCTTGCA GAGCCGGCAC 
CGAGAGGAGC TTGAGCAGGG TGAGAAGGCT GTGGCGGCGC TGCAGGCAGA GCTTCTTCGG 
GCCCGCCGAG AGCTTGGAGA CCTCACTGCA TTGCGACAGA AAGTGACTGA GCAGGAGCAG 
ATGGCTCAGC AGCTGAGGGC AGAGAATGCC AGCTATGCTG ACCAACTGAG TGCACTACAA 
CAGGCCCATA GCAGGCTAGC AGAGGAGAAC CGAAACCTGG GGGAGCGGGC CAGCCACGGG 
CGGCAGCAGC TGGAGGCTGA GCTTGGACGA GCCCAGGAAC TTGAGGCAGT GCGGGCTGAG 
GCAGAAGCCC AGGCAGTTCG GAGCCGGGAG GAGGCCCTAG ATACAGCCAG GCAGCTGGAG 
ACCATGACGG CCAAGTATGA GAGTGCCAAG AGCAAGGTCT TGGAGGAGAG GCAGAGGTTC 
CAGGAAGAGC GGCAGAAACT CACTGCCCAG GTGGAGCAAC TAGAGGCATT TCAGAGAGAA 
CAGACTAAAC AGGTGGAGGA ACTGAATAAG AAGCTGGCCC AGCAACAAAA TATGAAGCCC 
CCTGAAAGTG AAATACAGCA GCAAGCTCAT CATTACCAGG CCCAACTCAG TGAGCTGCAG 
GCCCAACTGA GCCAGAAGGA ACAGGCTGCT GAGCACTATA AGGTGCAGAT GGAAAGAGCC 
AAGGCTCACT ATGATGCCAA GAAGCAGCAG AACCAGGAGC TGCAGGAACG TCTGCAGAGC 
TTGGAGAATC TACAGAAGGA GAATGCTGAA CTGCGGACCG AGTCTGAGAA GCTCGGCTGG 
GAACTGAAGC AAGCTGGGCT GAAGACCAAG GAAGCTGAGC AAGCCTGCCG CCATCTTACT 
GCCCAGGTTC GAACTCTGGA GGCACAGGTT GCTCATGCAG ATCAGCAGCT CCGAGATCTT 
GGCAAGTTTC AAGTGGCAAC AGATGCACTG AAAACCCGAG AAGTCCGAGG GCTGCCCCAA 
CTGGATCTGA GCACTGACAG CCTAGAATTG AGCAGTGAGG AAGGGACCCC ACTGACCTCT 
ACAAGATCAG CAGCTCACCG TACTCGAAAG TCATCTCGAA CCCAGCCTGA TGGCACCAGC 
CTCCCTGGGG AGCCAGCTTC ACCCATCTCT CAGCGCCTGC CTTCTAAGGT GGAGTCCCTG 
GAGAGCTTGT ACTTCACCCC AATACCTGCC CGAGGACAGG CCCCCTTAGA AAGCAGCATG 
GACTCACTGG GTGACATTGT ATTGGATTCT GGTCCCAAGA CCCGCTCAGC CCGAAGGCGG 
ACCACACAGA TCATTGACAT CACAATGCTG TCCAGGAAAC TGGACACAGA TGAACCGGAC 
AGTTCGAATT CATCCTTCTA CAGCACACAG TCAGCCCCTG CCCCACAGCG GGGTCTCCGT 
TCTGCGTCTT CGGCTCAGTC CCTGGCTAAC TGTCCCTCTC GGGAGTCCCG GACCAGGCTG 
GGCTCCCTGG AAGATGGGAA CTCAGCCTTG CTCAGCCTTC CTGGTTATAG GCCCAACACT 
CGAAGCTCAG CCCGGCACTC CCAGGCCAGA GCATCTAGCG GGGCCCCTTC AGGTCGGAGC 
AGCTTCTATA CAGGCACCTG CCAGGATGAG CCAGAGCAGC TGGATGACTG GAACCGGATT 
GCAGAACTTC AGCAGCGCAA CCGTGTCTGC CTCCCACACC TGAAGACCTC TTACCCCTTG 
GAGGCCAGGC CTTCCATGAG CCTGGCCACC ATCACAGACG AAGAGATGAA GACGGGAGAC 
CCCCAGGAGA CCCTTCGCCG AGCCAGCATG CAGCCAGCCC AGATCCATGA GTTTAGTGGC 
ACACATCGCC TTCCCTCAAG CATCACCACC CGGCAGCACC GGAAGCGCAT CTCCTCGGAG 
CTTGACCAAG GCCTCGGCAC CCCTGAGGCC AAGAAACCGA CCAGCTGTTT TCCTCGTCCC 
CTCACCCCCC GGGACAGACA TGAAGGCCGG AAGATGAGTG CCGCCGAGGG CCAGAAGAAG 
GGGGCCCCTG CCCCCGGCAA GCAGACCAAC AAGCGCCAGT CATTAGCCTT CAACATTCTG 
AACACACCCA AGAGACTGGG GAACAGCCTG CTCCGGGGAG CCACCAGGAA ATCTTCGGCT 
AAGACCTCCC CACTCCCCCG AAGTGGGACC CGCCGCTCTC CCCGAATTGC CACTACTACC 
ATCACCACTG GCAGCACCTC TCCCCGGGCC AGGGGCAAGG CAAAGCACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_007491049.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007491111.1 Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X4, mRNA.

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CloneID	OMc22044
Clone ID Related Accession (Same CDS sequence)	XM_007491049.1
Accession Version	XM_007491049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	nuclear mitotic apparatus protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTTTG GACTCTTGCC ACAGGGCTCC AAGTGTGCAG TGGGTTCACC ATCTGACATA 
ACCAATATGA CTCTTCATGC GACCAGGACA GCTGCACTCC TCTCTTGGGT GAACAGCCTA 
CAGATTGCTG AACCTGTGAC ATCAGTGCTA GAGCTCCAAG ACTGCAACAT CTTTATCCAG 
ATCATCAACC GCATCCATGG CACTGAAGAG GGGCAGCAGA TCCTGGAGCA GCCTCTGCTG 
GACAGACTCA ACTTCATCGC CAACTTTCTG CAGAAAAACT GCAAACATCG GTCCTCCTCA 
GAGAGCCTAG TGTCTGTGCA GAAGCTAGAA GATGGGGAGG AGTTGGAGTT GGCCAAGGTG 
ATCATGTTAC TCTTATACCA TTCCACCATG AGTTCCAAAA GTTTGAGGGA TTGGAACCAA 
TTTGACTACA AAATCCAGTC GGAGTTGGCT GCCATCCTTA AATTTGTGTT GGACCATGAA 
GATGGGTTCA GTCTGAATGA CAACTTAGAG AGTTTCCTTC AGAAAGCTCC TCTCCTTTCC 
AGTAGTCCTA GCACCAGCTC TGATGAGCTT TCCCCAATGC GTACCCAACA AACCAGGAAG 
GAAGTTCGAT TCTTGGAACT GCAGAAGATT GCCTCCTCAT CAGGTCTAAA CAACTTCTTG 
TCAGGGTCTC CTGCTTCTCC CATGGGTGAT ATCCTTCAGA CCCCCCAGTT CCAAATGAGA 
CGGTTGAAGA AACAGCTGGC AGATGAGCGG GCTAGCCGAG ATGAGTTGGA GCTGGAGTTA 
GCCAAAAGCT GCCAACTACT GGCTGAGAAG GACACACAGA TTTCCACGAT GCAGCAGCGT 
ATTGATCGCT TGGCTCTCCT AAATGAGAAG CAGGCCACAG GACCCCTGGA ATCTAAAGAG 
CTGGAAGAAT TACGTGACAA GAATGAGAGT CTGATTGTAC GGTTTCATGA AGCCTTGAAA 
CAGTGCCAGG ACTTGAAGAC TGAGAAGGGC CAGATGGAGC GCAAGATTGA CCAGCTCTCT 
GAGGAGAATG GGGACCTTTC TTTCAAGCTT CGGGATTTTG CAAGCCATCT GCTGCAGCTA 
CAGGAGGCTC TCAATGATCT GACAGAAGAA CATAATAGCT CCACAAAGGA ATGGAGGGAG 
AAACAGGTTC ACCTGGAGAC AGAACTTGGC ACCACCCTGC AAGACAAGAA ATGCCTTGAA 
GAGAAGAATG AGATCCTCCA GGGAAAAATA TCACAGTTGG AGGAGCAGCT GGCACAGCTG 
GAAGCCCTGA AACAAGAAGT AGCCAACTTT GCTGCAAAGG ATGCTGAGCT TCAAGCCACA 
ATAAAGCAAC TGGAGGATGA ACGGAATCAG TGGAAATTAG AGATGCAAGC TGAGCAGGAC 
CGCTTTAAGG AAGAGAAGCA GCAGCTGGAG CAGAAGGCTC AGACTCAGGA AGCCCAGCTG 
ACTGCTCAGG TGGCCACCCT CCATGCCACC CTCCAGCAGC AGGACCATGA TCTGGCTGAC 
CTCAAGCAGC AGACTGAAAA GGAACAAGCA CAACTGACTG AGACCCTTCA ACAACAAGAG 
CTGGCTTCCC AAGGCCTCCA GCGCCAGGTG GAGCAACTGA GCAACAAGCT AAAGCAAGAG 
GAGGGAAGGC TGGAGGGACT GGTCCAGGAT TGGAAGGTGA CCAGCCAGAC CAAGGACCAA 
CAGCTGGCAG CAGCTATGGA GAAGTACCAG GAGGCCCAGC AAGAGAGGGA TGCAGCCCTG 
AAACAGTTGA AGGAGTTGGA GAAAACGAAA GCTGTGGAGT TGGGAGCTCT GCAACAGCAG 
CTCCAAGCTA CCAAGGATGC CTGTAACAGT GCCCAGTCTT GTGCTGCAAA GGCTGAGCAG 
GAGAAGACCA TACTGAGCCA GAAGGTGGAG GAGCTTAGCA CGTGTGTAGT TGAACTGAGG 
ACCTGGCAAG AGGAGCACAA AGCAGAAGCT AAATTGGCAG AAAGCCTTCA GACTCGGGTC 
GAGGAACTGG AGAACCAGCT GAAGGCTGAA CAACAGAAAC TAGCAGAGAG AGAAGAACTA 
GCTCGGGAGA ATGCCCAGCT CCAGGAACGA CTTCTTTCCC TGGAAGATTC GTTGCAGAAT 
ACTAAGGGCA TCCTGGAAGA TGAGAAGCGA CAAGCTGTAG AGGCCCTGGA GGGGCAACTA 
CGCCGCATTT CTGAGCTGGA GGCAGAGACG CAGAGCCTTG GGCAGCAGCG AGACCAAGAT 
CGGCGGCAGC TGGAGGAGGC AAAGGCTGGG CATCAGGGTC TGGAGGCCCA GCTCCAGAAG 
CTTGGAGAAG AGCATCGTTC CAAAGTAGAA ACCTTGCAGC AGGAACTGGA AAAGGTGGCA 
GCTGCCCGGC AAGAGACAGA GGGAAAGTGC AAGCAGCTGG CCTCAGAGGC AGCTTCCTGG 
CGTACTCGTT ATGAAGAGAA CCAGAAGGAG GGGGCGCAGC GTGGTGCCCT GCATGAGGAA 
CAGTTGAAGG CCCTGAATGA GGAGCAAGAG AAAACCCAAA AGCAGCTGCA GGCTGCCCAG 
GAGAGATTGG CAGTGCTGGA GGCTCTCACC AAACGCCAGG ATGCTAAACA GCAAGAATAC 
ACTCGGCTTG AGGCTGACCT GTCAGGTGCC CTCCGGCAGC TCCAGGAGAA GGAAGAGCGG 
GCCCAGGATC TGGCCGACAG CCTGTGCAAG CTGCGGGAGG AGGTAGATCA GCTCACAGCT 
GCAGTCAGGA AAGCAGATGA GGAGAAGGAA GCCTCTTCTC AGAGGCTGGC TGAAGAGCAG 
CAACGGGTGC AAGAGCTTGA GGCTGCCACA AAGCAGCTGG AAGAGCAGCA GAACCAGAAA 
CTAAGCAGGA TCCAGGCGGC CCTGCAGGCT AAGGAGCAAG AAGCAGAGCA GCTGACAGGA 
AAGCTAGAGC AAGCACGCGG TGCTCTGGCA GAGAGCCGGG GGCACCAGGA GGAAGAGCGT 
GTCCAGTGGG AGCGGGAAAG AGTCCGTGCA GAGGTGGAAC GAGACCAGGC CAGAGCCGAC 
CTGGCCACTG AGAAGGCTGC TAAGGCAGAA CTGGAGGCAA GGCTGCAGAG CTCCCTGAGT 
GAGCAGCGGG TGGAGTTCAG TGCCCTGCAG GAAGCCTTGG CCCATGCCCT GGAAGGAGCA 
GAAGGGAAGG AACAGCAGCT TGGAGAGCTT CAGAAGCAAG AGGCCGCCCT GCGGGCAGAA 
CTGACTAAGC TGCAGAAGAC CACTGAGGAC TTGAGAGAAA GGTTGGCTCA GGAGGAGGCA 
GCTGCCCAAG CAGATGCTCT GGGGCCTGCA CAGTCGGGCG CCCAGGAGTC AGAGGCTCTG 
CGGGCCGAGC TGGGTGAGCT GGAGCAGCGG TGCCGGGAGC AGGAGGAGAA GGCCTCTGAG 
CTGGAGCGCC GCCTTGAGGC TTCTCGGAAG GCCCAGGCCA GTCAGGAAAA CACTACTGCT 
GCCCTCCGGG CTCAGCTGGA GGAAAGCACC CAAGAGCTAG CTGCCCTCCG AGTGGCAGCT 
CAGGAGCACA GCAAGGCTGA AGAGGAGCAA AAAGCCCAGG TGGCCCATGC CCAGCAGGAG 
GCAGAGAAGA ATAGCAGCCT CATCAGCAGC TTGGAGCAGG AGGTGTCCAT CTTGAACCTA 
CAGGTTCTGG AGAAAGAAGG AGAAAGCAAA GAGCTGAAGC GTCTAGTGCT GGCTGAGTCA 
GAGAAAAGCC AGAAGCTGGA GGAGCGCCTG CGCCTGCTTC AGACAGAAAC TGCCAGTGCC 
AGCACACGGG CGGCAGAGTG CAGCGCAGCC CTGAGCTCTG AGGTGGAGGC GCTCCGGGAA 
GAGGCTGAAA AGCAGCGAGC GGCTGCAGAT GGTCTCCGGA GGGAGCTGGC CTCCCAGGCT 
GAGCATGCAG AGGGCCTGGG ACAGGAACTG AAGACTTGGC GTGAGAGGCT ATCCCAGAAG 
GAGCAGTCCC TGTCCTCACT GCAGCAAGAG CTTGCCACCT CCAAGGCCCT GGTTGGGGAA 
CTGTTGCCCA TCAAGCACCT CTGCCAGCAG TTAGAGGCCG AGAAGGGCAC CTTGCAGAGC 
CGGCACCGAG AGGAGCTTGA GCAGGGTGAG AAGGCTGTGG CGGCGCTGCA GGCAGAGCTT 
CTTCGGGCCC GCCGAGAGCT TGGAGACCTC ACTGCATTGC GACAGAAAGT GACTGAGCAG 
GAGCAGATGG CTCAGCAGCT GAGGGCAGAG AATGCCAGCT ATGCTGACCA ACTGAGTGCA 
CTACAACAGG CCCATAGCAG GCTAGCAGAG GAGAACCGAA ACCTGGGGGA GCGGGCCAGC 
CACGGGCGGC AGCAGCTGGA GGCTGAGCTT GGACGAGCCC AGGAACTTGA GGCAGTGCGG 
GCTGAGGCAG AAGCCCAGGC AGTTCGGAGC CGGGAGGAGG CCCTAGATAC AGCCAGGCAG 
CTGGAGACCA TGACGGCCAA GTATGAGAGT GCCAAGAGCA AGGTCTTGGA GGAGAGGCAG 
AGGTTCCAGG AAGAGCGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGCATTTCAG 
AGAGAACAGA CTAAACAGGT GGAGGAACTG AATAAGAAGC TGGCCCAGCA ACAAAATATG 
AAGCCCCCTG AAAGTGAAAT ACAGCAGCAA GCTCATCATT ACCAGGCCCA ACTCAGTGAG 
CTGCAGGCCC AACTGAGCCA GAAGGAACAG GCTGCTGAGC ACTATAAGGT GCAGATGGAA 
AGAGCCAAGG CTCACTATGA TGCCAAGAAG CAGCAGAACC AGGAGCTGCA GGAACGTCTG 
CAGAGCTTGG AGAATCTACA GAAGGAGAAT GCTGAACTGC GGACCGAGTC TGAGAAGCTC 
GGCTGGGAAC TGAAGCAAGC TGGGCTGAAG ACCAAGGAAG CTGAGCAAGC CTGCCGCCAT 
CTTACTGCCC AGGTTCGAAC TCTGGAGGCA CAGGTTGCTC ATGCAGATCA GCAGCTCCGA 
GATCTTGGCA AGTTTCAAGT GGCAACAGAT GCACTGAAAA CCCGAGAAGT CCGAGGGCTG 
CCCCAACTGG ATCTGAGCAC TGACAGCCTA GAATTGAGCA GTGAGGAAGG GACCCCACTG 
ACCTCTACAA GATCAGCAGC TCACCGTACT CGAAAGTCAT CTCGAACCCA GCCTGATGGC 
ACCAGCCTCC CTGGGGAGCC AGCTTCACCC ATCTCTCAGC GCCTGCCTTC TAAGGTGGAG 
TCCCTGGAGA GCTTGTACTT CACCCCAATA CCTGCCCGAG GACAGGCCCC CTTAGAAAGC 
AGCATGGACT CACTGGGTGA CATTGTATTG GATTCTGGTC CCAAGACCCG CTCAGCCCGA 
AGGCGGACCA CACAGATCAT TGACATCACA ATGCTGTCCA GGAAACTGGA CACAGATGAA 
CCGGACAGTT CGAATTCATC CTTCTACAGC ACACAGTCAG CCCCTGCCCC ACAGCGGGGT 
CTCCGTTCTG CGTCTTCGGC TCAGTCCCTG GCTAACTGTC CCTCTCGGGA GTCCCGGACC 
AGGCTGGGCT CCCTGGAAGA TGGGAACTCA GCCTTGCTCA GCCTTCCTGG TTATAGGCCC 
AACACTCGAA GCTCAGCCCG GCACTCCCAG GCCAGAGCAT CTAGCGGGGC CCCTTCAGGT 
CGGAGCAGCT TCTATACAGG CACCTGCCAG GATGAGCCAG AGCAGCTGGA TGACTGGAAC 
CGGATTGCAG AACTTCAGCA GCGCAACCGT GTCTGCCTCC CACACCTGAA GACCTCTTAC 
CCCTTGGAGG CCAGGCCTTC CATGAGCCTG GCCACCATCA CAGACGAAGA GATGAAGACG 
GGAGACCCCC AGGAGACCCT TCGCCGAGCC AGCATGCAGC CAGCCCAGAT CCATGAGTTT 
AGTGGCACAC ATCGCCTTCC CTCAAGCATC ACCACCCGGC AGCACCGGAA GCGCATCTCC 
TCGGAGCTTG ACCAAGGCCT CGGCACCCCT GAGGCCAAGA AACCGACCAG CTGTTTTCCT 
CGTCCCCTCA CCCCCCGGGA CAGACATGAA GGCCGGAAGA TGAGTGCCGC CGAGGGCCAG 
AAGAAGGGGG CCCCTGCCCC CGGCAAGCAG ACCAACAAGC GCCAGTCATT AGCCTTCAAC 
ATTCTGAACA CACCCAAGAG ACTGGGGAAC AGCCTGCTCC GGGGAGCCAC CAGGAAATCT 
TCGGCTAAGA CCTCCCCACT CCCCCGAAGT GGGACCCGCC GCTCTCCCCG AATTGCCACT 
ACTACCATCA CCACTGGCAG CACCTCTCCC CGGGCCAGGG GCAAGGCAAA GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007491111.1
CDS	299..6535
Translation	MSFGLLPQGSKCAVGSPSDITNMTLHATRTAALLSWVNSLQIAEPVTSVLELQDCNIFIQIINRIHGTEEGQQILEQPLLDRLNFIANFLQKNCKHRSSSESLVSVQKLEDGEELELAKVIMLLLYHSTMSSKSLRDWNQFDYKIQSELAAILKFVLDHEDGFSLNDNLESFLQKAPLLSSSPSTSSDELSPMRTQQTRKEVRFLELQKIASSSGLNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERASRDELELELAKSCQLLAEKDTQISTMQQRIDRLALLNEKQATGPLESKELEELRDKNESLIVRFHEALKQCQDLKTEKGQMERKIDQLSEENGDLSFKLRDFASHLLQLQEALNDLTEEHNSSTKEWREKQVHLETELGTTLQDKKCLEEKNEILQGKISQLEEQLAQLEALKQEVANFAAKDAELQATIKQLEDERNQWKLEMQAEQDRFKEEKQQLEQKAQTQEAQLTAQVATLHATLQQQDHDLADLKQQTEKEQAQLTETLQQQELASQGLQRQVEQLSNKLKQEEGRLEGLVQDWKVTSQTKDQQLAAAMEKYQEAQQERDAALKQLKELEKTKAVELGALQQQLQATKDACNSAQSCAAKAEQEKTILSQKVEELSTCVVELRTWQEEHKAEAKLAESLQTRVEELENQLKAEQQKLAEREELARENAQLQERLLSLEDSLQNTKGILEDEKRQAVEALEGQLRRISELEAETQSLGQQRDQDRRQLEEAKAGHQGLEAQLQKLGEEHRSKVETLQQELEKVAAARQETEGKCKQLASEAASWRTRYEENQKEGAQRGALHEEQLKALNEEQEKTQKQLQAAQERLAVLEALTKRQDAKQQEYTRLEADLSGALRQLQEKEERAQDLADSLCKLREEVDQLTAAVRKADEEKEASSQRLAEEQQRVQELEAATKQLEEQQNQKLSRIQAALQAKEQEAEQLTGKLEQARGALAESRGHQEEERVQWERERVRAEVERDQARADLATEKAAKAELEARLQSSLSEQRVEFSALQEALAHALEGAEGKEQQLGELQKQEAALRAELTKLQKTTEDLRERLAQEEAAAQADALGPAQSGAQESEALRAELGELEQRCREQEEKASELERRLEASRKAQASQENTTAALRAQLEESTQELAALRVAAQEHSKAEEEQKAQVAHAQQEAEKNSSLISSLEQEVSILNLQVLEKEGESKELKRLVLAESEKSQKLEERLRLLQTETASASTRAAECSAALSSEVEALREEAEKQRAAADGLRRELASQAEHAEGLGQELKTWRERLSQKEQSLSSLQQELATSKALVGELLPIKHLCQQLEAEKGTLQSRHREELEQGEKAVAALQAELLRARRELGDLTALRQKVTEQEQMAQQLRAENASYADQLSALQQAHSRLAEENRNLGERASHGRQQLEAELGRAQELEAVRAEAEAQAVRSREEALDTARQLETMTAKYESAKSKVLEERQRFQEERQKLTAQVEQLEAFQREQTKQVEELNKKLAQQQNMKPPESEIQQQAHHYQAQLSELQAQLSQKEQAAEHYKVQMERAKAHYDAKKQQNQELQERLQSLENLQKENAELRTESEKLGWELKQAGLKTKEAEQACRHLTAQVRTLEAQVAHADQQLRDLGKFQVATDALKTREVRGLPQLDLSTDSLELSSEEGTPLTSTRSAAHRTRKSSRTQPDGTSLPGEPASPISQRLPSKVESLESLYFTPIPARGQAPLESSMDSLGDIVLDSGPKTRSARRRTTQIIDITMLSRKLDTDEPDSSNSSFYSTQSAPAPQRGLRSASSAQSLANCPSRESRTRLGSLEDGNSALLSLPGYRPNTRSSARHSQARASSGAPSGRSSFYTGTCQDEPEQLDDWNRIAELQQRNRVCLPHLKTSYPLEARPSMSLATITDEEMKTGDPQETLRRASMQPAQIHEFSGTHRLPSSITTRQHRKRISSELDQGLGTPEAKKPTSCFPRPLTPRDRHEGRKMSAAEGQKKGAPAPGKQTNKRQSLAFNILNTPKRLGNSLLRGATRKSSAKTSPLPRSGTRRSPRIATTTITTGSTSPRARGKAKH

Target ORF information:

RefSeq Version	XM_007491049.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007491049.1

ORF Insert Sequence:

ATGAGCTTTG GACTCTTGCC ACAGGGCTCC AAGTGTGCAG TGGGTTCACC ATCTGACATA 
ACCAATATGA CTCTTCATGC GACCAGGACA GCTGCACTCC TCTCTTGGGT GAACAGCCTA 
CAGATTGCTG AACCTGTGAC ATCAGTGCTA GAGCTCCAAG ACTGCAACAT CTTTATCCAG 
ATCATCAACC GCATCCATGG CACTGAAGAG GGGCAGCAGA TCCTGGAGCA GCCTCTGCTG 
GACAGACTCA ACTTCATCGC CAACTTTCTG CAGAAAAACT GCAAACATCG GTCCTCCTCA 
GAGAGCCTAG TGTCTGTGCA GAAGCTAGAA GATGGGGAGG AGTTGGAGTT GGCCAAGGTG 
ATCATGTTAC TCTTATACCA TTCCACCATG AGTTCCAAAA GTTTGAGGGA TTGGAACCAA 
TTTGACTACA AAATCCAGTC GGAGTTGGCT GCCATCCTTA AATTTGTGTT GGACCATGAA 
GATGGGTTCA GTCTGAATGA CAACTTAGAG AGTTTCCTTC AGAAAGCTCC TCTCCTTTCC 
AGTAGTCCTA GCACCAGCTC TGATGAGCTT TCCCCAATGC GTACCCAACA AACCAGGAAG 
GAAGTTCGAT TCTTGGAACT GCAGAAGATT GCCTCCTCAT CAGGTCTAAA CAACTTCTTG 
TCAGGGTCTC CTGCTTCTCC CATGGGTGAT ATCCTTCAGA CCCCCCAGTT CCAAATGAGA 
CGGTTGAAGA AACAGCTGGC AGATGAGCGG GCTAGCCGAG ATGAGTTGGA GCTGGAGTTA 
GCCAAAAGCT GCCAACTACT GGCTGAGAAG GACACACAGA TTTCCACGAT GCAGCAGCGT 
ATTGATCGCT TGGCTCTCCT AAATGAGAAG CAGGCCACAG GACCCCTGGA ATCTAAAGAG 
CTGGAAGAAT TACGTGACAA GAATGAGAGT CTGATTGTAC GGTTTCATGA AGCCTTGAAA 
CAGTGCCAGG ACTTGAAGAC TGAGAAGGGC CAGATGGAGC GCAAGATTGA CCAGCTCTCT 
GAGGAGAATG GGGACCTTTC TTTCAAGCTT CGGGATTTTG CAAGCCATCT GCTGCAGCTA 
CAGGAGGCTC TCAATGATCT GACAGAAGAA CATAATAGCT CCACAAAGGA ATGGAGGGAG 
AAACAGGTTC ACCTGGAGAC AGAACTTGGC ACCACCCTGC AAGACAAGAA ATGCCTTGAA 
GAGAAGAATG AGATCCTCCA GGGAAAAATA TCACAGTTGG AGGAGCAGCT GGCACAGCTG 
GAAGCCCTGA AACAAGAAGT AGCCAACTTT GCTGCAAAGG ATGCTGAGCT TCAAGCCACA 
ATAAAGCAAC TGGAGGATGA ACGGAATCAG TGGAAATTAG AGATGCAAGC TGAGCAGGAC 
CGCTTTAAGG AAGAGAAGCA GCAGCTGGAG CAGAAGGCTC AGACTCAGGA AGCCCAGCTG 
ACTGCTCAGG TGGCCACCCT CCATGCCACC CTCCAGCAGC AGGACCATGA TCTGGCTGAC 
CTCAAGCAGC AGACTGAAAA GGAACAAGCA CAACTGACTG AGACCCTTCA ACAACAAGAG 
CTGGCTTCCC AAGGCCTCCA GCGCCAGGTG GAGCAACTGA GCAACAAGCT AAAGCAAGAG 
GAGGGAAGGC TGGAGGGACT GGTCCAGGAT TGGAAGGTGA CCAGCCAGAC CAAGGACCAA 
CAGCTGGCAG CAGCTATGGA GAAGTACCAG GAGGCCCAGC AAGAGAGGGA TGCAGCCCTG 
AAACAGTTGA AGGAGTTGGA GAAAACGAAA GCTGTGGAGT TGGGAGCTCT GCAACAGCAG 
CTCCAAGCTA CCAAGGATGC CTGTAACAGT GCCCAGTCTT GTGCTGCAAA GGCTGAGCAG 
GAGAAGACCA TACTGAGCCA GAAGGTGGAG GAGCTTAGCA CGTGTGTAGT TGAACTGAGG 
ACCTGGCAAG AGGAGCACAA AGCAGAAGCT AAATTGGCAG AAAGCCTTCA GACTCGGGTC 
GAGGAACTGG AGAACCAGCT GAAGGCTGAA CAACAGAAAC TAGCAGAGAG AGAAGAACTA 
GCTCGGGAGA ATGCCCAGCT CCAGGAACGA CTTCTTTCCC TGGAAGATTC GTTGCAGAAT 
ACTAAGGGCA TCCTGGAAGA TGAGAAGCGA CAAGCTGTAG AGGCCCTGGA GGGGCAACTA 
CGCCGCATTT CTGAGCTGGA GGCAGAGACG CAGAGCCTTG GGCAGCAGCG AGACCAAGAT 
CGGCGGCAGC TGGAGGAGGC AAAGGCTGGG CATCAGGGTC TGGAGGCCCA GCTCCAGAAG 
CTTGGAGAAG AGCATCGTTC CAAAGTAGAA ACCTTGCAGC AGGAACTGGA AAAGGTGGCA 
GCTGCCCGGC AAGAGACAGA GGGAAAGTGC AAGCAGCTGG CCTCAGAGGC AGCTTCCTGG 
CGTACTCGTT ATGAAGAGAA CCAGAAGGAG GGGGCGCAGC GTGGTGCCCT GCATGAGGAA 
CAGTTGAAGG CCCTGAATGA GGAGCAAGAG AAAACCCAAA AGCAGCTGCA GGCTGCCCAG 
GAGAGATTGG CAGTGCTGGA GGCTCTCACC AAACGCCAGG ATGCTAAACA GCAAGAATAC 
ACTCGGCTTG AGGCTGACCT GTCAGGTGCC CTCCGGCAGC TCCAGGAGAA GGAAGAGCGG 
GCCCAGGATC TGGCCGACAG CCTGTGCAAG CTGCGGGAGG AGGTAGATCA GCTCACAGCT 
GCAGTCAGGA AAGCAGATGA GGAGAAGGAA GCCTCTTCTC AGAGGCTGGC TGAAGAGCAG 
CAACGGGTGC AAGAGCTTGA GGCTGCCACA AAGCAGCTGG AAGAGCAGCA GAACCAGAAA 
CTAAGCAGGA TCCAGGCGGC CCTGCAGGCT AAGGAGCAAG AAGCAGAGCA GCTGACAGGA 
AAGCTAGAGC AAGCACGCGG TGCTCTGGCA GAGAGCCGGG GGCACCAGGA GGAAGAGCGT 
GTCCAGTGGG AGCGGGAAAG AGTCCGTGCA GAGGTGGAAC GAGACCAGGC CAGAGCCGAC 
CTGGCCACTG AGAAGGCTGC TAAGGCAGAA CTGGAGGCAA GGCTGCAGAG CTCCCTGAGT 
GAGCAGCGGG TGGAGTTCAG TGCCCTGCAG GAAGCCTTGG CCCATGCCCT GGAAGGAGCA 
GAAGGGAAGG AACAGCAGCT TGGAGAGCTT CAGAAGCAAG AGGCCGCCCT GCGGGCAGAA 
CTGACTAAGC TGCAGAAGAC CACTGAGGAC TTGAGAGAAA GGTTGGCTCA GGAGGAGGCA 
GCTGCCCAAG CAGATGCTCT GGGGCCTGCA CAGTCGGGCG CCCAGGAGTC AGAGGCTCTG 
CGGGCCGAGC TGGGTGAGCT GGAGCAGCGG TGCCGGGAGC AGGAGGAGAA GGCCTCTGAG 
CTGGAGCGCC GCCTTGAGGC TTCTCGGAAG GCCCAGGCCA GTCAGGAAAA CACTACTGCT 
GCCCTCCGGG CTCAGCTGGA GGAAAGCACC CAAGAGCTAG CTGCCCTCCG AGTGGCAGCT 
CAGGAGCACA GCAAGGCTGA AGAGGAGCAA AAAGCCCAGG TGGCCCATGC CCAGCAGGAG 
GCAGAGAAGA ATAGCAGCCT CATCAGCAGC TTGGAGCAGG AGGTGTCCAT CTTGAACCTA 
CAGGTTCTGG AGAAAGAAGG AGAAAGCAAA GAGCTGAAGC GTCTAGTGCT GGCTGAGTCA 
GAGAAAAGCC AGAAGCTGGA GGAGCGCCTG CGCCTGCTTC AGACAGAAAC TGCCAGTGCC 
AGCACACGGG CGGCAGAGTG CAGCGCAGCC CTGAGCTCTG AGGTGGAGGC GCTCCGGGAA 
GAGGCTGAAA AGCAGCGAGC GGCTGCAGAT GGTCTCCGGA GGGAGCTGGC CTCCCAGGCT 
GAGCATGCAG AGGGCCTGGG ACAGGAACTG AAGACTTGGC GTGAGAGGCT ATCCCAGAAG 
GAGCAGTCCC TGTCCTCACT GCAGCAAGAG CTTGCCACCT CCAAGGCCCT GGTTGGGGAA 
CTGTTGCCCA TCAAGCACCT CTGCCAGCAG TTAGAGGCCG AGAAGGGCAC CTTGCAGAGC 
CGGCACCGAG AGGAGCTTGA GCAGGGTGAG AAGGCTGTGG CGGCGCTGCA GGCAGAGCTT 
CTTCGGGCCC GCCGAGAGCT TGGAGACCTC ACTGCATTGC GACAGAAAGT GACTGAGCAG 
GAGCAGATGG CTCAGCAGCT GAGGGCAGAG AATGCCAGCT ATGCTGACCA ACTGAGTGCA 
CTACAACAGG CCCATAGCAG GCTAGCAGAG GAGAACCGAA ACCTGGGGGA GCGGGCCAGC 
CACGGGCGGC AGCAGCTGGA GGCTGAGCTT GGACGAGCCC AGGAACTTGA GGCAGTGCGG 
GCTGAGGCAG AAGCCCAGGC AGTTCGGAGC CGGGAGGAGG CCCTAGATAC AGCCAGGCAG 
CTGGAGACCA TGACGGCCAA GTATGAGAGT GCCAAGAGCA AGGTCTTGGA GGAGAGGCAG 
AGGTTCCAGG AAGAGCGGCA GAAACTCACT GCCCAGGTGG AGCAACTAGA GGCATTTCAG 
AGAGAACAGA CTAAACAGGT GGAGGAACTG AATAAGAAGC TGGCCCAGCA ACAAAATATG 
AAGCCCCCTG AAAGTGAAAT ACAGCAGCAA GCTCATCATT ACCAGGCCCA ACTCAGTGAG 
CTGCAGGCCC AACTGAGCCA GAAGGAACAG GCTGCTGAGC ACTATAAGGT GCAGATGGAA 
AGAGCCAAGG CTCACTATGA TGCCAAGAAG CAGCAGAACC AGGAGCTGCA GGAACGTCTG 
CAGAGCTTGG AGAATCTACA GAAGGAGAAT GCTGAACTGC GGACCGAGTC TGAGAAGCTC 
GGCTGGGAAC TGAAGCAAGC TGGGCTGAAG ACCAAGGAAG CTGAGCAAGC CTGCCGCCAT 
CTTACTGCCC AGGTTCGAAC TCTGGAGGCA CAGGTTGCTC ATGCAGATCA GCAGCTCCGA 
GATCTTGGCA AGTTTCAAGT GGCAACAGAT GCACTGAAAA CCCGAGAAGT CCGAGGGCTG 
CCCCAACTGG ATCTGAGCAC TGACAGCCTA GAATTGAGCA GTGAGGAAGG GACCCCACTG 
ACCTCTACAA GATCAGCAGC TCACCGTACT CGAAAGTCAT CTCGAACCCA GCCTGATGGC 
ACCAGCCTCC CTGGGGAGCC AGCTTCACCC ATCTCTCAGC GCCTGCCTTC TAAGGTGGAG 
TCCCTGGAGA GCTTGTACTT CACCCCAATA CCTGCCCGAG GACAGGCCCC CTTAGAAAGC 
AGCATGGACT CACTGGGTGA CATTGTATTG GATTCTGGTC CCAAGACCCG CTCAGCCCGA 
AGGCGGACCA CACAGATCAT TGACATCACA ATGCTGTCCA GGAAACTGGA CACAGATGAA 
CCGGACAGTT CGAATTCATC CTTCTACAGC ACACAGTCAG CCCCTGCCCC ACAGCGGGGT 
CTCCGTTCTG CGTCTTCGGC TCAGTCCCTG GCTAACTGTC CCTCTCGGGA GTCCCGGACC 
AGGCTGGGCT CCCTGGAAGA TGGGAACTCA GCCTTGCTCA GCCTTCCTGG TTATAGGCCC 
AACACTCGAA GCTCAGCCCG GCACTCCCAG GCCAGAGCAT CTAGCGGGGC CCCTTCAGGT 
CGGAGCAGCT TCTATACAGG CACCTGCCAG GATGAGCCAG AGCAGCTGGA TGACTGGAAC 
CGGATTGCAG AACTTCAGCA GCGCAACCGT GTCTGCCTCC CACACCTGAA GACCTCTTAC 
CCCTTGGAGG CCAGGCCTTC CATGAGCCTG GCCACCATCA CAGACGAAGA GATGAAGACG 
GGAGACCCCC AGGAGACCCT TCGCCGAGCC AGCATGCAGC CAGCCCAGAT CCATGAGTTT 
AGTGGCACAC ATCGCCTTCC CTCAAGCATC ACCACCCGGC AGCACCGGAA GCGCATCTCC 
TCGGAGCTTG ACCAAGGCCT CGGCACCCCT GAGGCCAAGA AACCGACCAG CTGTTTTCCT 
CGTCCCCTCA CCCCCCGGGA CAGACATGAA GGCCGGAAGA TGAGTGCCGC CGAGGGCCAG 
AAGAAGGGGG CCCCTGCCCC CGGCAAGCAG ACCAACAAGC GCCAGTCATT AGCCTTCAAC 
ATTCTGAACA CACCCAAGAG ACTGGGGAAC AGCCTGCTCC GGGGAGCCAC CAGGAAATCT 
TCGGCTAAGA CCTCCCCACT CCCCCGAAGT GGGACCCGCC GCTCTCCCCG AATTGCCACT 
ACTACCATCA CCACTGGCAG CACCTCTCCC CGGGCCAGGG GCAAGGCAAA GCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_007491050.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007491112.1 Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X5, mRNA.

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CloneID	OMc22045
Clone ID Related Accession (Same CDS sequence)	XM_007491050.1 , XM_007491051.2 , XM_007491053.2 , XM_007491052.1
Accession Version	XM_007491050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	nuclear mitotic apparatus protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCTTC ATGCGACCAG GACAGCTGCA CTCCTCTCTT GGGTGAACAG CCTACAGATT 
GCTGAACCTG TGACATCAGT GCTAGAGCTC CAAGACTGCA ACATCTTTAT CCAGATCATC 
AACCGCATCC ATGGCACTGA AGAGGGGCAG CAGATCCTGG AGCAGCCTCT GCTGGACAGA 
CTCAACTTCA TCGCCAACTT TCTGCAGAAA AACTGCAAAC ATCGGTCCTC CTCAGAGAGC 
CTAGTGTCTG TGCAGAAGCT AGAAGATGGG GAGGAGTTGG AGTTGGCCAA GGTGATCATG 
TTACTCTTAT ACCATTCCAC CATGAGTTCC AAAAGTTTGA GGGATTGGAA CCAATTTGAC 
TACAAAATCC AGTCGGAGTT GGCTGCCATC CTTAAATTTG TGTTGGACCA TGAAGATGGG 
TTCAGTCTGA ATGACAACTT AGAGAGTTTC CTTCAGAAAG CTCCTCTCCT TTCCAGTAGT 
CCTAGCACCA GCTCTGATGA GCTTTCCCCA ATGCGTACCC AACAAACCAG GAAGGAAGTT 
CGATTCTTGG AACTGCAGAA GATTGCCTCC TCATCAGGTC TAAACAACTT CTTGTCAGGG 
TCTCCTGCTT CTCCCATGGG TGATATCCTT CAGACCCCCC AGTTCCAAAT GAGACGGTTG 
AAGAAACAGC TGGCAGATGA GCGGGCTAGC CGAGATGAGT TGGAGCTGGA GTTAGCCAAA 
AGCTGCCAAC TACTGGCTGA GAAGGACACA CAGATTTCCA CGATGCAGCA GCGTATTGAT 
CGCTTGGCTC TCCTAAATGA GAAGCAGGCC ACAGGACCCC TGGAATCTAA AGAGCTGGAA 
GAATTACGTG ACAAGAATGA GAGTCTGATT GTACGGTTTC ATGAAGCCTT GAAACAGTGC 
CAGGACTTGA AGACTGAGAA GGGCCAGATG GAGCGCAAGA TTGACCAGCT CTCTGAGGAG 
AATGGGGACC TTTCTTTCAA GCTTCGGGAT TTTGCAAGCC ATCTGCTGCA GCTACAGGAG 
GCTCTCAATG ATCTGACAGA AGAACATAAT AGCTCCACAA AGGAATGGAG GGAGAAACAG 
GTTCACCTGG AGACAGAACT TGGCACCACC CTGCAAGACA AGAAATGCCT TGAAGAGAAG 
AATGAGATCC TCCAGGGAAA AATATCACAG TTGGAGGAGC AGCTGGCACA GGTTGGGAAG 
AATCCATCTC AAGAGAAGGG AGAGGTGTTG GGCGACATCT TGCAGCTGGA AGCCCTGAAA 
CAAGAAGTAG CCAACTTTGC TGCAAAGGAT GCTGAGCTTC AAGCCACAAT AAAGCAACTG 
GAGGATGAAC GGAATCAGTG GAAATTAGAG ATGCAAGCTG AGCAGGACCG CTTTAAGGAA 
GAGAAGCAGC AGCTGGAGCA GAAGGCTCAG ACTCAGGAAG CCCAGCTGAC TGCTCAGGTG 
GCCACCCTCC ATGCCACCCT CCAGCAGCAG GACCATGATC TGGCTGACCT CAAGCAGCAG 
ACTGAAAAGG AACAAGCACA ACTGACTGAG ACCCTTCAAC AACAAGAGCT GGCTTCCCAA 
GGCCTCCAGC GCCAGGTGGA GCAACTGAGC AACAAGCTAA AGCAAGAGGA GGGAAGGCTG 
GAGGGACTGG TCCAGGATTG GAAGGTGACC AGCCAGACCA AGGACCAACA GCTGGCAGCA 
GCTATGGAGA AGTACCAGGA GGCCCAGCAA GAGAGGGATG CAGCCCTGAA ACAGTTGAAG 
GAGTTGGAGA AAACGAAAGC TGTGGAGTTG GGAGCTCTGC AACAGCAGCT CCAAGCTACC 
AAGGATGCCT GTAACAGTGC CCAGTCTTGT GCTGCAAAGG CTGAGCAGGA GAAGACCATA 
CTGAGCCAGA AGGTGGAGGA GCTTAGCACG TGTGTAGTTG AACTGAGGAC CTGGCAAGAG 
GAGCACAAAG CAGAAGCTAA ATTGGCAGAA AGCCTTCAGA CTCGGGTCGA GGAACTGGAG 
AACCAGCTGA AGGCTGAACA ACAGAAACTA GCAGAGAGAG AAGAACTAGC TCGGGAGAAT 
GCCCAGCTCC AGGAACGACT TCTTTCCCTG GAAGATTCGT TGCAGAATAC TAAGGGCATC 
CTGGAAGATG AGAAGCGACA AGCTGTAGAG GCCCTGGAGG GGCAACTACG CCGCATTTCT 
GAGCTGGAGG CAGAGACGCA GAGCCTTGGG CAGCAGCGAG ACCAAGATCG GCGGCAGCTG 
GAGGAGGCAA AGGCTGGGCA TCAGGGTCTG GAGGCCCAGC TCCAGAAGCT TGGAGAAGAG 
CATCGTTCCA AAGTAGAAAC CTTGCAGCAG GAACTGGAAA AGGTGGCAGC TGCCCGGCAA 
GAGACAGAGG GAAAGTGCAA GCAGCTGGCC TCAGAGGCAG CTTCCTGGCG TACTCGTTAT 
GAAGAGAACC AGAAGGAGGG GGCGCAGCGT GGTGCCCTGC ATGAGGAACA GTTGAAGGCC 
CTGAATGAGG AGCAAGAGAA AACCCAAAAG CAGCTGCAGG CTGCCCAGGA GAGATTGGCA 
GTGCTGGAGG CTCTCACCAA ACGCCAGGAT GCTAAACAGC AAGAATACAC TCGGCTTGAG 
GCTGACCTGT CAGGTGCCCT CCGGCAGCTC CAGGAGAAGG AAGAGCGGGC CCAGGATCTG 
GCCGACAGCC TGTGCAAGCT GCGGGAGGAG GTAGATCAGC TCACAGCTGC AGTCAGGAAA 
GCAGATGAGG AGAAGGAAGC CTCTTCTCAG AGGCTGGCTG AAGAGCAGCA ACGGGTGCAA 
GAGCTTGAGG CTGCCACAAA GCAGCTGGAA GAGCAGCAGA ACCAGAAACT AAGCAGGATC 
CAGGCGGCCC TGCAGGCTAA GGAGCAAGAA GCAGAGCAGC TGACAGGAAA GCTAGAGCAA 
GCACGCGGTG CTCTGGCAGA GAGCCGGGGG CACCAGGAGG AAGAGCGTGT CCAGTGGGAG 
CGGGAAAGAG TCCGTGCAGA GGTGGAACGA GACCAGGCCA GAGCCGACCT GGCCACTGAG 
AAGGCTGCTA AGGCAGAACT GGAGGCAAGG CTGCAGAGCT CCCTGAGTGA GCAGCGGGTG 
GAGTTCAGTG CCCTGCAGGA AGCCTTGGCC CATGCCCTGG AAGGAGCAGA AGGGAAGGAA 
CAGCAGCTTG GAGAGCTTCA GAAGCAAGAG GCCGCCCTGC GGGCAGAACT GACTAAGCTG 
CAGAAGACCA CTGAGGACTT GAGAGAAAGG TTGGCTCAGG AGGAGGCAGC TGCCCAAGCA 
GATGCTCTGG GGCCTGCACA GTCGGGCGCC CAGGAGTCAG AGGCTCTGCG GGCCGAGCTG 
GGTGAGCTGG AGCAGCGGTG CCGGGAGCAG GAGGAGAAGG CCTCTGAGCT GGAGCGCCGC 
CTTGAGGCTT CTCGGAAGGC CCAGGCCAGT CAGGAAAACA CTACTGCTGC CCTCCGGGCT 
CAGCTGGAGG AAAGCACCCA AGAGCTAGCT GCCCTCCGAG TGGCAGCTCA GGAGCACAGC 
AAGGCTGAAG AGGAGCAAAA AGCCCAGGTG GCCCATGCCC AGCAGGAGGC AGAGAAGAAT 
AGCAGCCTCA TCAGCAGCTT GGAGCAGGAG GTGTCCATCT TGAACCTACA GGTTCTGGAG 
AAAGAAGGAG AAAGCAAAGA GCTGAAGCGT CTAGTGCTGG CTGAGTCAGA GAAAAGCCAG 
AAGCTGGAGG AGCGCCTGCG CCTGCTTCAG ACAGAAACTG CCAGTGCCAG CACACGGGCG 
GCAGAGTGCA GCGCAGCCCT GAGCTCTGAG GTGGAGGCGC TCCGGGAAGA GGCTGAAAAG 
CAGCGAGCGG CTGCAGATGG TCTCCGGAGG GAGCTGGCCT CCCAGGCTGA GCATGCAGAG 
GGCCTGGGAC AGGAACTGAA GACTTGGCGT GAGAGGCTAT CCCAGAAGGA GCAGTCCCTG 
TCCTCACTGC AGCAAGAGCT TGCCACCTCC AAGGCCCTGG TTGGGGAACT GTTGCCCATC 
AAGCACCTCT GCCAGCAGTT AGAGGCCGAG AAGGGCACCT TGCAGAGCCG GCACCGAGAG 
GAGCTTGAGC AGGGTGAGAA GGCTGTGGCG GCGCTGCAGG CAGAGCTTCT TCGGGCCCGC 
CGAGAGCTTG GAGACCTCAC TGCATTGCGA CAGAAAGTGA CTGAGCAGGA GCAGATGGCT 
CAGCAGCTGA GGGCAGAGAA TGCCAGCTAT GCTGACCAAC TGAGTGCACT ACAACAGGCC 
CATAGCAGGC TAGCAGAGGA GAACCGAAAC CTGGGGGAGC GGGCCAGCCA CGGGCGGCAG 
CAGCTGGAGG CTGAGCTTGG ACGAGCCCAG GAACTTGAGG CAGTGCGGGC TGAGGCAGAA 
GCCCAGGCAG TTCGGAGCCG GGAGGAGGCC CTAGATACAG CCAGGCAGCT GGAGACCATG 
ACGGCCAAGT ATGAGAGTGC CAAGAGCAAG GTCTTGGAGG AGAGGCAGAG GTTCCAGGAA 
GAGCGGCAGA AACTCACTGC CCAGGTGGAG CAACTAGAGG CATTTCAGAG AGAACAGACT 
AAACAGGTGG AGGAACTGAA TAAGAAGCTG GCCCAGCAAC AAAATATGAA GCCCCCTGAA 
AGTGAAATAC AGCAGCAAGC TCATCATTAC CAGGCCCAAC TCAGTGAGCT GCAGGCCCAA 
CTGAGCCAGA AGGAACAGGC TGCTGAGCAC TATAAGGTGC AGATGGAAAG AGCCAAGGCT 
CACTATGATG CCAAGAAGCA GCAGAACCAG GAGCTGCAGG AACGTCTGCA GAGCTTGGAG 
AATCTACAGA AGGAGAATGC TGAACTGCGG ACCGAGTCTG AGAAGCTCGG CTGGGAACTG 
AAGCAAGCTG GGCTGAAGAC CAAGGAAGCT GAGCAAGCCT GCCGCCATCT TACTGCCCAG 
GTTCGAACTC TGGAGGCACA GGTTGCTCAT GCAGATCAGC AGCTCCGAGA TCTTGGCAAG 
TTTCAAGTGG CAACAGATGC ACTGAAAACC CGAGAAGTCC GAGGGCTGCC CCAACTGGAT 
CTGAGCACTG ACAGCCTAGA ATTGAGCAGT GAGGAAGGGA CCCCACTGAC CTCTACAAGA 
TCAGCAGCTC ACCGTACTCG AAAGTCATCT CGAACCCAGC CTGATGGCAC CAGCCTCCCT 
GGGGAGCCAG CTTCACCCAT CTCTCAGCGC CTGCCTTCTA AGGTGGAGTC CCTGGAGAGC 
TTGTACTTCA CCCCAATACC TGCCCGAGGA CAGGCCCCCT TAGAAAGCAG CATGGACTCA 
CTGGGTGACA TTGTATTGGA TTCTGGTCCC AAGACCCGCT CAGCCCGAAG GCGGACCACA 
CAGATCATTG ACATCACAAT GCTGTCCAGG AAACTGGACA CAGATGAACC GGACAGTTCG 
AATTCATCCT TCTACAGCAC ACAGTCAGCC CCTGCCCCAC AGCGGGGTCT CCGTTCTGCG 
TCTTCGGCTC AGTCCCTGGC TAACTGTCCC TCTCGGGAGT CCCGGACCAG GCTGGGCTCC 
CTGGAAGATG GGAACTCAGC CTTGCTCAGC CTTCCTGGTT ATAGGCCCAA CACTCGAAGC 
TCAGCCCGGC ACTCCCAGGC CAGAGCATCT AGCGGGGCCC CTTCAGGTCG GAGCAGCTTC 
TATACAGGCA CCTGCCAGGA TGAGCCAGAG CAGCTGGATG ACTGGAACCG GATTGCAGAA 
CTTCAGCAGC GCAACCGTGT CTGCCTCCCA CACCTGAAGA CCTCTTACCC CTTGGAGGCC 
AGGCCTTCCA TGAGCCTGGC CACCATCACA GACGAAGAGA TGAAGACGGG AGACCCCCAG 
GAGACCCTTC GCCGAGCCAG CATGCAGCCA GCCCAGATCC ATGAGTTTAG TGGCACACAT 
CGCCTTCCCT CAAGCATCAC CACCCGGCAG CACCGGAAGC GCATCTCCTC GGAGCTTGAC 
CAAGGCCTCG GCACCCCTGA GGCCAAGAAA CCGACCAGCT GTTTTCCTCG TCCCCTCACC 
CCCCGGGACA GACATGAAGG CCGGAAGATG AGTGCCGCCG AGGGCCAGAA GAAGGGGGCC 
CCTGCCCCCG GCAAGCAGAC CAACAAGCGC CAGTCATTAG CCTTCAACAT TCTGAACACA 
CCCAAGAGAC TGGGGAACAG CCTGCTCCGG GGAGCCACCA GGAAATCTTC GGCTAAGACC 
TCCCCACTCC CCCGAAGTGG GACCCGCCGC TCTCCCCGAA TTGCCACTAC TACCATCACC 
ACTGGCAGCA CCTCTCCCCG GGCCAGGGGC AAGGCAAAGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007491112.1
CDS	274..6498
Translation	MTLHATRTAALLSWVNSLQIAEPVTSVLELQDCNIFIQIINRIHGTEEGQQILEQPLLDRLNFIANFLQKNCKHRSSSESLVSVQKLEDGEELELAKVIMLLLYHSTMSSKSLRDWNQFDYKIQSELAAILKFVLDHEDGFSLNDNLESFLQKAPLLSSSPSTSSDELSPMRTQQTRKEVRFLELQKIASSSGLNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERASRDELELELAKSCQLLAEKDTQISTMQQRIDRLALLNEKQATGPLESKELEELRDKNESLIVRFHEALKQCQDLKTEKGQMERKIDQLSEENGDLSFKLRDFASHLLQLQEALNDLTEEHNSSTKEWREKQVHLETELGTTLQDKKCLEEKNEILQGKISQLEEQLAQVGKNPSQEKGEVLGDILQLEALKQEVANFAAKDAELQATIKQLEDERNQWKLEMQAEQDRFKEEKQQLEQKAQTQEAQLTAQVATLHATLQQQDHDLADLKQQTEKEQAQLTETLQQQELASQGLQRQVEQLSNKLKQEEGRLEGLVQDWKVTSQTKDQQLAAAMEKYQEAQQERDAALKQLKELEKTKAVELGALQQQLQATKDACNSAQSCAAKAEQEKTILSQKVEELSTCVVELRTWQEEHKAEAKLAESLQTRVEELENQLKAEQQKLAEREELARENAQLQERLLSLEDSLQNTKGILEDEKRQAVEALEGQLRRISELEAETQSLGQQRDQDRRQLEEAKAGHQGLEAQLQKLGEEHRSKVETLQQELEKVAAARQETEGKCKQLASEAASWRTRYEENQKEGAQRGALHEEQLKALNEEQEKTQKQLQAAQERLAVLEALTKRQDAKQQEYTRLEADLSGALRQLQEKEERAQDLADSLCKLREEVDQLTAAVRKADEEKEASSQRLAEEQQRVQELEAATKQLEEQQNQKLSRIQAALQAKEQEAEQLTGKLEQARGALAESRGHQEEERVQWERERVRAEVERDQARADLATEKAAKAELEARLQSSLSEQRVEFSALQEALAHALEGAEGKEQQLGELQKQEAALRAELTKLQKTTEDLRERLAQEEAAAQADALGPAQSGAQESEALRAELGELEQRCREQEEKASELERRLEASRKAQASQENTTAALRAQLEESTQELAALRVAAQEHSKAEEEQKAQVAHAQQEAEKNSSLISSLEQEVSILNLQVLEKEGESKELKRLVLAESEKSQKLEERLRLLQTETASASTRAAECSAALSSEVEALREEAEKQRAAADGLRRELASQAEHAEGLGQELKTWRERLSQKEQSLSSLQQELATSKALVGELLPIKHLCQQLEAEKGTLQSRHREELEQGEKAVAALQAELLRARRELGDLTALRQKVTEQEQMAQQLRAENASYADQLSALQQAHSRLAEENRNLGERASHGRQQLEAELGRAQELEAVRAEAEAQAVRSREEALDTARQLETMTAKYESAKSKVLEERQRFQEERQKLTAQVEQLEAFQREQTKQVEELNKKLAQQQNMKPPESEIQQQAHHYQAQLSELQAQLSQKEQAAEHYKVQMERAKAHYDAKKQQNQELQERLQSLENLQKENAELRTESEKLGWELKQAGLKTKEAEQACRHLTAQVRTLEAQVAHADQQLRDLGKFQVATDALKTREVRGLPQLDLSTDSLELSSEEGTPLTSTRSAAHRTRKSSRTQPDGTSLPGEPASPISQRLPSKVESLESLYFTPIPARGQAPLESSMDSLGDIVLDSGPKTRSARRRTTQIIDITMLSRKLDTDEPDSSNSSFYSTQSAPAPQRGLRSASSAQSLANCPSRESRTRLGSLEDGNSALLSLPGYRPNTRSSARHSQARASSGAPSGRSSFYTGTCQDEPEQLDDWNRIAELQQRNRVCLPHLKTSYPLEARPSMSLATITDEEMKTGDPQETLRRASMQPAQIHEFSGTHRLPSSITTRQHRKRISSELDQGLGTPEAKKPTSCFPRPLTPRDRHEGRKMSAAEGQKKGAPAPGKQTNKRQSLAFNILNTPKRLGNSLLRGATRKSSAKTSPLPRSGTRRSPRIATTTITTGSTSPRARGKAKH

Target ORF information:

RefSeq Version	XM_007491050.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007491050.1

ORF Insert Sequence:

ATGACTCTTC ATGCGACCAG GACAGCTGCA CTCCTCTCTT GGGTGAACAG CCTACAGATT 
GCTGAACCTG TGACATCAGT GCTAGAGCTC CAAGACTGCA ACATCTTTAT CCAGATCATC 
AACCGCATCC ATGGCACTGA AGAGGGGCAG CAGATCCTGG AGCAGCCTCT GCTGGACAGA 
CTCAACTTCA TCGCCAACTT TCTGCAGAAA AACTGCAAAC ATCGGTCCTC CTCAGAGAGC 
CTAGTGTCTG TGCAGAAGCT AGAAGATGGG GAGGAGTTGG AGTTGGCCAA GGTGATCATG 
TTACTCTTAT ACCATTCCAC CATGAGTTCC AAAAGTTTGA GGGATTGGAA CCAATTTGAC 
TACAAAATCC AGTCGGAGTT GGCTGCCATC CTTAAATTTG TGTTGGACCA TGAAGATGGG 
TTCAGTCTGA ATGACAACTT AGAGAGTTTC CTTCAGAAAG CTCCTCTCCT TTCCAGTAGT 
CCTAGCACCA GCTCTGATGA GCTTTCCCCA ATGCGTACCC AACAAACCAG GAAGGAAGTT 
CGATTCTTGG AACTGCAGAA GATTGCCTCC TCATCAGGTC TAAACAACTT CTTGTCAGGG 
TCTCCTGCTT CTCCCATGGG TGATATCCTT CAGACCCCCC AGTTCCAAAT GAGACGGTTG 
AAGAAACAGC TGGCAGATGA GCGGGCTAGC CGAGATGAGT TGGAGCTGGA GTTAGCCAAA 
AGCTGCCAAC TACTGGCTGA GAAGGACACA CAGATTTCCA CGATGCAGCA GCGTATTGAT 
CGCTTGGCTC TCCTAAATGA GAAGCAGGCC ACAGGACCCC TGGAATCTAA AGAGCTGGAA 
GAATTACGTG ACAAGAATGA GAGTCTGATT GTACGGTTTC ATGAAGCCTT GAAACAGTGC 
CAGGACTTGA AGACTGAGAA GGGCCAGATG GAGCGCAAGA TTGACCAGCT CTCTGAGGAG 
AATGGGGACC TTTCTTTCAA GCTTCGGGAT TTTGCAAGCC ATCTGCTGCA GCTACAGGAG 
GCTCTCAATG ATCTGACAGA AGAACATAAT AGCTCCACAA AGGAATGGAG GGAGAAACAG 
GTTCACCTGG AGACAGAACT TGGCACCACC CTGCAAGACA AGAAATGCCT TGAAGAGAAG 
AATGAGATCC TCCAGGGAAA AATATCACAG TTGGAGGAGC AGCTGGCACA GGTTGGGAAG 
AATCCATCTC AAGAGAAGGG AGAGGTGTTG GGCGACATCT TGCAGCTGGA AGCCCTGAAA 
CAAGAAGTAG CCAACTTTGC TGCAAAGGAT GCTGAGCTTC AAGCCACAAT AAAGCAACTG 
GAGGATGAAC GGAATCAGTG GAAATTAGAG ATGCAAGCTG AGCAGGACCG CTTTAAGGAA 
GAGAAGCAGC AGCTGGAGCA GAAGGCTCAG ACTCAGGAAG CCCAGCTGAC TGCTCAGGTG 
GCCACCCTCC ATGCCACCCT CCAGCAGCAG GACCATGATC TGGCTGACCT CAAGCAGCAG 
ACTGAAAAGG AACAAGCACA ACTGACTGAG ACCCTTCAAC AACAAGAGCT GGCTTCCCAA 
GGCCTCCAGC GCCAGGTGGA GCAACTGAGC AACAAGCTAA AGCAAGAGGA GGGAAGGCTG 
GAGGGACTGG TCCAGGATTG GAAGGTGACC AGCCAGACCA AGGACCAACA GCTGGCAGCA 
GCTATGGAGA AGTACCAGGA GGCCCAGCAA GAGAGGGATG CAGCCCTGAA ACAGTTGAAG 
GAGTTGGAGA AAACGAAAGC TGTGGAGTTG GGAGCTCTGC AACAGCAGCT CCAAGCTACC 
AAGGATGCCT GTAACAGTGC CCAGTCTTGT GCTGCAAAGG CTGAGCAGGA GAAGACCATA 
CTGAGCCAGA AGGTGGAGGA GCTTAGCACG TGTGTAGTTG AACTGAGGAC CTGGCAAGAG 
GAGCACAAAG CAGAAGCTAA ATTGGCAGAA AGCCTTCAGA CTCGGGTCGA GGAACTGGAG 
AACCAGCTGA AGGCTGAACA ACAGAAACTA GCAGAGAGAG AAGAACTAGC TCGGGAGAAT 
GCCCAGCTCC AGGAACGACT TCTTTCCCTG GAAGATTCGT TGCAGAATAC TAAGGGCATC 
CTGGAAGATG AGAAGCGACA AGCTGTAGAG GCCCTGGAGG GGCAACTACG CCGCATTTCT 
GAGCTGGAGG CAGAGACGCA GAGCCTTGGG CAGCAGCGAG ACCAAGATCG GCGGCAGCTG 
GAGGAGGCAA AGGCTGGGCA TCAGGGTCTG GAGGCCCAGC TCCAGAAGCT TGGAGAAGAG 
CATCGTTCCA AAGTAGAAAC CTTGCAGCAG GAACTGGAAA AGGTGGCAGC TGCCCGGCAA 
GAGACAGAGG GAAAGTGCAA GCAGCTGGCC TCAGAGGCAG CTTCCTGGCG TACTCGTTAT 
GAAGAGAACC AGAAGGAGGG GGCGCAGCGT GGTGCCCTGC ATGAGGAACA GTTGAAGGCC 
CTGAATGAGG AGCAAGAGAA AACCCAAAAG CAGCTGCAGG CTGCCCAGGA GAGATTGGCA 
GTGCTGGAGG CTCTCACCAA ACGCCAGGAT GCTAAACAGC AAGAATACAC TCGGCTTGAG 
GCTGACCTGT CAGGTGCCCT CCGGCAGCTC CAGGAGAAGG AAGAGCGGGC CCAGGATCTG 
GCCGACAGCC TGTGCAAGCT GCGGGAGGAG GTAGATCAGC TCACAGCTGC AGTCAGGAAA 
GCAGATGAGG AGAAGGAAGC CTCTTCTCAG AGGCTGGCTG AAGAGCAGCA ACGGGTGCAA 
GAGCTTGAGG CTGCCACAAA GCAGCTGGAA GAGCAGCAGA ACCAGAAACT AAGCAGGATC 
CAGGCGGCCC TGCAGGCTAA GGAGCAAGAA GCAGAGCAGC TGACAGGAAA GCTAGAGCAA 
GCACGCGGTG CTCTGGCAGA GAGCCGGGGG CACCAGGAGG AAGAGCGTGT CCAGTGGGAG 
CGGGAAAGAG TCCGTGCAGA GGTGGAACGA GACCAGGCCA GAGCCGACCT GGCCACTGAG 
AAGGCTGCTA AGGCAGAACT GGAGGCAAGG CTGCAGAGCT CCCTGAGTGA GCAGCGGGTG 
GAGTTCAGTG CCCTGCAGGA AGCCTTGGCC CATGCCCTGG AAGGAGCAGA AGGGAAGGAA 
CAGCAGCTTG GAGAGCTTCA GAAGCAAGAG GCCGCCCTGC GGGCAGAACT GACTAAGCTG 
CAGAAGACCA CTGAGGACTT GAGAGAAAGG TTGGCTCAGG AGGAGGCAGC TGCCCAAGCA 
GATGCTCTGG GGCCTGCACA GTCGGGCGCC CAGGAGTCAG AGGCTCTGCG GGCCGAGCTG 
GGTGAGCTGG AGCAGCGGTG CCGGGAGCAG GAGGAGAAGG CCTCTGAGCT GGAGCGCCGC 
CTTGAGGCTT CTCGGAAGGC CCAGGCCAGT CAGGAAAACA CTACTGCTGC CCTCCGGGCT 
CAGCTGGAGG AAAGCACCCA AGAGCTAGCT GCCCTCCGAG TGGCAGCTCA GGAGCACAGC 
AAGGCTGAAG AGGAGCAAAA AGCCCAGGTG GCCCATGCCC AGCAGGAGGC AGAGAAGAAT 
AGCAGCCTCA TCAGCAGCTT GGAGCAGGAG GTGTCCATCT TGAACCTACA GGTTCTGGAG 
AAAGAAGGAG AAAGCAAAGA GCTGAAGCGT CTAGTGCTGG CTGAGTCAGA GAAAAGCCAG 
AAGCTGGAGG AGCGCCTGCG CCTGCTTCAG ACAGAAACTG CCAGTGCCAG CACACGGGCG 
GCAGAGTGCA GCGCAGCCCT GAGCTCTGAG GTGGAGGCGC TCCGGGAAGA GGCTGAAAAG 
CAGCGAGCGG CTGCAGATGG TCTCCGGAGG GAGCTGGCCT CCCAGGCTGA GCATGCAGAG 
GGCCTGGGAC AGGAACTGAA GACTTGGCGT GAGAGGCTAT CCCAGAAGGA GCAGTCCCTG 
TCCTCACTGC AGCAAGAGCT TGCCACCTCC AAGGCCCTGG TTGGGGAACT GTTGCCCATC 
AAGCACCTCT GCCAGCAGTT AGAGGCCGAG AAGGGCACCT TGCAGAGCCG GCACCGAGAG 
GAGCTTGAGC AGGGTGAGAA GGCTGTGGCG GCGCTGCAGG CAGAGCTTCT TCGGGCCCGC 
CGAGAGCTTG GAGACCTCAC TGCATTGCGA CAGAAAGTGA CTGAGCAGGA GCAGATGGCT 
CAGCAGCTGA GGGCAGAGAA TGCCAGCTAT GCTGACCAAC TGAGTGCACT ACAACAGGCC 
CATAGCAGGC TAGCAGAGGA GAACCGAAAC CTGGGGGAGC GGGCCAGCCA CGGGCGGCAG 
CAGCTGGAGG CTGAGCTTGG ACGAGCCCAG GAACTTGAGG CAGTGCGGGC TGAGGCAGAA 
GCCCAGGCAG TTCGGAGCCG GGAGGAGGCC CTAGATACAG CCAGGCAGCT GGAGACCATG 
ACGGCCAAGT ATGAGAGTGC CAAGAGCAAG GTCTTGGAGG AGAGGCAGAG GTTCCAGGAA 
GAGCGGCAGA AACTCACTGC CCAGGTGGAG CAACTAGAGG CATTTCAGAG AGAACAGACT 
AAACAGGTGG AGGAACTGAA TAAGAAGCTG GCCCAGCAAC AAAATATGAA GCCCCCTGAA 
AGTGAAATAC AGCAGCAAGC TCATCATTAC CAGGCCCAAC TCAGTGAGCT GCAGGCCCAA 
CTGAGCCAGA AGGAACAGGC TGCTGAGCAC TATAAGGTGC AGATGGAAAG AGCCAAGGCT 
CACTATGATG CCAAGAAGCA GCAGAACCAG GAGCTGCAGG AACGTCTGCA GAGCTTGGAG 
AATCTACAGA AGGAGAATGC TGAACTGCGG ACCGAGTCTG AGAAGCTCGG CTGGGAACTG 
AAGCAAGCTG GGCTGAAGAC CAAGGAAGCT GAGCAAGCCT GCCGCCATCT TACTGCCCAG 
GTTCGAACTC TGGAGGCACA GGTTGCTCAT GCAGATCAGC AGCTCCGAGA TCTTGGCAAG 
TTTCAAGTGG CAACAGATGC ACTGAAAACC CGAGAAGTCC GAGGGCTGCC CCAACTGGAT 
CTGAGCACTG ACAGCCTAGA ATTGAGCAGT GAGGAAGGGA CCCCACTGAC CTCTACAAGA 
TCAGCAGCTC ACCGTACTCG AAAGTCATCT CGAACCCAGC CTGATGGCAC CAGCCTCCCT 
GGGGAGCCAG CTTCACCCAT CTCTCAGCGC CTGCCTTCTA AGGTGGAGTC CCTGGAGAGC 
TTGTACTTCA CCCCAATACC TGCCCGAGGA CAGGCCCCCT TAGAAAGCAG CATGGACTCA 
CTGGGTGACA TTGTATTGGA TTCTGGTCCC AAGACCCGCT CAGCCCGAAG GCGGACCACA 
CAGATCATTG ACATCACAAT GCTGTCCAGG AAACTGGACA CAGATGAACC GGACAGTTCG 
AATTCATCCT TCTACAGCAC ACAGTCAGCC CCTGCCCCAC AGCGGGGTCT CCGTTCTGCG 
TCTTCGGCTC AGTCCCTGGC TAACTGTCCC TCTCGGGAGT CCCGGACCAG GCTGGGCTCC 
CTGGAAGATG GGAACTCAGC CTTGCTCAGC CTTCCTGGTT ATAGGCCCAA CACTCGAAGC 
TCAGCCCGGC ACTCCCAGGC CAGAGCATCT AGCGGGGCCC CTTCAGGTCG GAGCAGCTTC 
TATACAGGCA CCTGCCAGGA TGAGCCAGAG CAGCTGGATG ACTGGAACCG GATTGCAGAA 
CTTCAGCAGC GCAACCGTGT CTGCCTCCCA CACCTGAAGA CCTCTTACCC CTTGGAGGCC 
AGGCCTTCCA TGAGCCTGGC CACCATCACA GACGAAGAGA TGAAGACGGG AGACCCCCAG 
GAGACCCTTC GCCGAGCCAG CATGCAGCCA GCCCAGATCC ATGAGTTTAG TGGCACACAT 
CGCCTTCCCT CAAGCATCAC CACCCGGCAG CACCGGAAGC GCATCTCCTC GGAGCTTGAC 
CAAGGCCTCG GCACCCCTGA GGCCAAGAAA CCGACCAGCT GTTTTCCTCG TCCCCTCACC 
CCCCGGGACA GACATGAAGG CCGGAAGATG AGTGCCGCCG AGGGCCAGAA GAAGGGGGCC 
CCTGCCCCCG GCAAGCAGAC CAACAAGCGC CAGTCATTAG CCTTCAACAT TCTGAACACA 
CCCAAGAGAC TGGGGAACAG CCTGCTCCGG GGAGCCACCA GGAAATCTTC GGCTAAGACC 
TCCCCACTCC CCCGAAGTGG GACCCGCCGC TCTCCCCGAA TTGCCACTAC TACCATCACC 
ACTGGCAGCA CCTCTCCCCG GGCCAGGGGC AAGGCAAAGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_007491051.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007491113.1 Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X6, mRNA.

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CloneID	OMc22045
Clone ID Related Accession (Same CDS sequence)	XM_007491050.1 , XM_007491051.2 , XM_007491053.2 , XM_007491052.1
Accession Version	XM_007491051.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	nuclear mitotic apparatus protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007491051.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCTTC ATGCGACCAG GACAGCTGCA CTCCTCTCTT GGGTGAACAG CCTACAGATT 
GCTGAACCTG TGACATCAGT GCTAGAGCTC CAAGACTGCA ACATCTTTAT CCAGATCATC 
AACCGCATCC ATGGCACTGA AGAGGGGCAG CAGATCCTGG AGCAGCCTCT GCTGGACAGA 
CTCAACTTCA TCGCCAACTT TCTGCAGAAA AACTGCAAAC ATCGGTCCTC CTCAGAGAGC 
CTAGTGTCTG TGCAGAAGCT AGAAGATGGG GAGGAGTTGG AGTTGGCCAA GGTGATCATG 
TTACTCTTAT ACCATTCCAC CATGAGTTCC AAAAGTTTGA GGGATTGGAA CCAATTTGAC 
TACAAAATCC AGTCGGAGTT GGCTGCCATC CTTAAATTTG TGTTGGACCA TGAAGATGGG 
TTCAGTCTGA ATGACAACTT AGAGAGTTTC CTTCAGAAAG CTCCTCTCCT TTCCAGTAGT 
CCTAGCACCA GCTCTGATGA GCTTTCCCCA ATGCGTACCC AACAAACCAG GAAGGAAGTT 
CGATTCTTGG AACTGCAGAA GATTGCCTCC TCATCAGGTC TAAACAACTT CTTGTCAGGG 
TCTCCTGCTT CTCCCATGGG TGATATCCTT CAGACCCCCC AGTTCCAAAT GAGACGGTTG 
AAGAAACAGC TGGCAGATGA GCGGGCTAGC CGAGATGAGT TGGAGCTGGA GTTAGCCAAA 
AGCTGCCAAC TACTGGCTGA GAAGGACACA CAGATTTCCA CGATGCAGCA GCGTATTGAT 
CGCTTGGCTC TCCTAAATGA GAAGCAGGCC ACAGGACCCC TGGAATCTAA AGAGCTGGAA 
GAATTACGTG ACAAGAATGA GAGTCTGATT GTACGGTTTC ATGAAGCCTT GAAACAGTGC 
CAGGACTTGA AGACTGAGAA GGGCCAGATG GAGCGCAAGA TTGACCAGCT CTCTGAGGAG 
AATGGGGACC TTTCTTTCAA GCTTCGGGAT TTTGCAAGCC ATCTGCTGCA GCTACAGGAG 
GCTCTCAATG ATCTGACAGA AGAACATAAT AGCTCCACAA AGGAATGGAG GGAGAAACAG 
GTTCACCTGG AGACAGAACT TGGCACCACC CTGCAAGACA AGAAATGCCT TGAAGAGAAG 
AATGAGATCC TCCAGGGAAA AATATCACAG TTGGAGGAGC AGCTGGCACA GGTTGGGAAG 
AATCCATCTC AAGAGAAGGG AGAGGTGTTG GGCGACATCT TGCAGCTGGA AGCCCTGAAA 
CAAGAAGTAG CCAACTTTGC TGCAAAGGAT GCTGAGCTTC AAGCCACAAT AAAGCAACTG 
GAGGATGAAC GGAATCAGTG GAAATTAGAG ATGCAAGCTG AGCAGGACCG CTTTAAGGAA 
GAGAAGCAGC AGCTGGAGCA GAAGGCTCAG ACTCAGGAAG CCCAGCTGAC TGCTCAGGTG 
GCCACCCTCC ATGCCACCCT CCAGCAGCAG GACCATGATC TGGCTGACCT CAAGCAGCAG 
ACTGAAAAGG AACAAGCACA ACTGACTGAG ACCCTTCAAC AACAAGAGCT GGCTTCCCAA 
GGCCTCCAGC GCCAGGTGGA GCAACTGAGC AACAAGCTAA AGCAAGAGGA GGGAAGGCTG 
GAGGGACTGG TCCAGGATTG GAAGGTGACC AGCCAGACCA AGGACCAACA GCTGGCAGCA 
GCTATGGAGA AGTACCAGGA GGCCCAGCAA GAGAGGGATG CAGCCCTGAA ACAGTTGAAG 
GAGTTGGAGA AAACGAAAGC TGTGGAGTTG GGAGCTCTGC AACAGCAGCT CCAAGCTACC 
AAGGATGCCT GTAACAGTGC CCAGTCTTGT GCTGCAAAGG CTGAGCAGGA GAAGACCATA 
CTGAGCCAGA AGGTGGAGGA GCTTAGCACG TGTGTAGTTG AACTGAGGAC CTGGCAAGAG 
GAGCACAAAG CAGAAGCTAA ATTGGCAGAA AGCCTTCAGA CTCGGGTCGA GGAACTGGAG 
AACCAGCTGA AGGCTGAACA ACAGAAACTA GCAGAGAGAG AAGAACTAGC TCGGGAGAAT 
GCCCAGCTCC AGGAACGACT TCTTTCCCTG GAAGATTCGT TGCAGAATAC TAAGGGCATC 
CTGGAAGATG AGAAGCGACA AGCTGTAGAG GCCCTGGAGG GGCAACTACG CCGCATTTCT 
GAGCTGGAGG CAGAGACGCA GAGCCTTGGG CAGCAGCGAG ACCAAGATCG GCGGCAGCTG 
GAGGAGGCAA AGGCTGGGCA TCAGGGTCTG GAGGCCCAGC TCCAGAAGCT TGGAGAAGAG 
CATCGTTCCA AAGTAGAAAC CTTGCAGCAG GAACTGGAAA AGGTGGCAGC TGCCCGGCAA 
GAGACAGAGG GAAAGTGCAA GCAGCTGGCC TCAGAGGCAG CTTCCTGGCG TACTCGTTAT 
GAAGAGAACC AGAAGGAGGG GGCGCAGCGT GGTGCCCTGC ATGAGGAACA GTTGAAGGCC 
CTGAATGAGG AGCAAGAGAA AACCCAAAAG CAGCTGCAGG CTGCCCAGGA GAGATTGGCA 
GTGCTGGAGG CTCTCACCAA ACGCCAGGAT GCTAAACAGC AAGAATACAC TCGGCTTGAG 
GCTGACCTGT CAGGTGCCCT CCGGCAGCTC CAGGAGAAGG AAGAGCGGGC CCAGGATCTG 
GCCGACAGCC TGTGCAAGCT GCGGGAGGAG GTAGATCAGC TCACAGCTGC AGTCAGGAAA 
GCAGATGAGG AGAAGGAAGC CTCTTCTCAG AGGCTGGCTG AAGAGCAGCA ACGGGTGCAA 
GAGCTTGAGG CTGCCACAAA GCAGCTGGAA GAGCAGCAGA ACCAGAAACT AAGCAGGATC 
CAGGCGGCCC TGCAGGCTAA GGAGCAAGAA GCAGAGCAGC TGACAGGAAA GCTAGAGCAA 
GCACGCGGTG CTCTGGCAGA GAGCCGGGGG CACCAGGAGG AAGAGCGTGT CCAGTGGGAG 
CGGGAAAGAG TCCGTGCAGA GGTGGAACGA GACCAGGCCA GAGCCGACCT GGCCACTGAG 
AAGGCTGCTA AGGCAGAACT GGAGGCAAGG CTGCAGAGCT CCCTGAGTGA GCAGCGGGTG 
GAGTTCAGTG CCCTGCAGGA AGCCTTGGCC CATGCCCTGG AAGGAGCAGA AGGGAAGGAA 
CAGCAGCTTG GAGAGCTTCA GAAGCAAGAG GCCGCCCTGC GGGCAGAACT GACTAAGCTG 
CAGAAGACCA CTGAGGACTT GAGAGAAAGG TTGGCTCAGG AGGAGGCAGC TGCCCAAGCA 
GATGCTCTGG GGCCTGCACA GTCGGGCGCC CAGGAGTCAG AGGCTCTGCG GGCCGAGCTG 
GGTGAGCTGG AGCAGCGGTG CCGGGAGCAG GAGGAGAAGG CCTCTGAGCT GGAGCGCCGC 
CTTGAGGCTT CTCGGAAGGC CCAGGCCAGT CAGGAAAACA CTACTGCTGC CCTCCGGGCT 
CAGCTGGAGG AAAGCACCCA AGAGCTAGCT GCCCTCCGAG TGGCAGCTCA GGAGCACAGC 
AAGGCTGAAG AGGAGCAAAA AGCCCAGGTG GCCCATGCCC AGCAGGAGGC AGAGAAGAAT 
AGCAGCCTCA TCAGCAGCTT GGAGCAGGAG GTGTCCATCT TGAACCTACA GGTTCTGGAG 
AAAGAAGGAG AAAGCAAAGA GCTGAAGCGT CTAGTGCTGG CTGAGTCAGA GAAAAGCCAG 
AAGCTGGAGG AGCGCCTGCG CCTGCTTCAG ACAGAAACTG CCAGTGCCAG CACACGGGCG 
GCAGAGTGCA GCGCAGCCCT GAGCTCTGAG GTGGAGGCGC TCCGGGAAGA GGCTGAAAAG 
CAGCGAGCGG CTGCAGATGG TCTCCGGAGG GAGCTGGCCT CCCAGGCTGA GCATGCAGAG 
GGCCTGGGAC AGGAACTGAA GACTTGGCGT GAGAGGCTAT CCCAGAAGGA GCAGTCCCTG 
TCCTCACTGC AGCAAGAGCT TGCCACCTCC AAGGCCCTGG TTGGGGAACT GTTGCCCATC 
AAGCACCTCT GCCAGCAGTT AGAGGCCGAG AAGGGCACCT TGCAGAGCCG GCACCGAGAG 
GAGCTTGAGC AGGGTGAGAA GGCTGTGGCG GCGCTGCAGG CAGAGCTTCT TCGGGCCCGC 
CGAGAGCTTG GAGACCTCAC TGCATTGCGA CAGAAAGTGA CTGAGCAGGA GCAGATGGCT 
CAGCAGCTGA GGGCAGAGAA TGCCAGCTAT GCTGACCAAC TGAGTGCACT ACAACAGGCC 
CATAGCAGGC TAGCAGAGGA GAACCGAAAC CTGGGGGAGC GGGCCAGCCA CGGGCGGCAG 
CAGCTGGAGG CTGAGCTTGG ACGAGCCCAG GAACTTGAGG CAGTGCGGGC TGAGGCAGAA 
GCCCAGGCAG TTCGGAGCCG GGAGGAGGCC CTAGATACAG CCAGGCAGCT GGAGACCATG 
ACGGCCAAGT ATGAGAGTGC CAAGAGCAAG GTCTTGGAGG AGAGGCAGAG GTTCCAGGAA 
GAGCGGCAGA AACTCACTGC CCAGGTGGAG CAACTAGAGG CATTTCAGAG AGAACAGACT 
AAACAGGTGG AGGAACTGAA TAAGAAGCTG GCCCAGCAAC AAAATATGAA GCCCCCTGAA 
AGTGAAATAC AGCAGCAAGC TCATCATTAC CAGGCCCAAC TCAGTGAGCT GCAGGCCCAA 
CTGAGCCAGA AGGAACAGGC TGCTGAGCAC TATAAGGTGC AGATGGAAAG AGCCAAGGCT 
CACTATGATG CCAAGAAGCA GCAGAACCAG GAGCTGCAGG AACGTCTGCA GAGCTTGGAG 
AATCTACAGA AGGAGAATGC TGAACTGCGG ACCGAGTCTG AGAAGCTCGG CTGGGAACTG 
AAGCAAGCTG GGCTGAAGAC CAAGGAAGCT GAGCAAGCCT GCCGCCATCT TACTGCCCAG 
GTTCGAACTC TGGAGGCACA GGTTGCTCAT GCAGATCAGC AGCTCCGAGA TCTTGGCAAG 
TTTCAAGTGG CAACAGATGC ACTGAAAACC CGAGAAGTCC GAGGGCTGCC CCAACTGGAT 
CTGAGCACTG ACAGCCTAGA ATTGAGCAGT GAGGAAGGGA CCCCACTGAC CTCTACAAGA 
TCAGCAGCTC ACCGTACTCG AAAGTCATCT CGAACCCAGC CTGATGGCAC CAGCCTCCCT 
GGGGAGCCAG CTTCACCCAT CTCTCAGCGC CTGCCTTCTA AGGTGGAGTC CCTGGAGAGC 
TTGTACTTCA CCCCAATACC TGCCCGAGGA CAGGCCCCCT TAGAAAGCAG CATGGACTCA 
CTGGGTGACA TTGTATTGGA TTCTGGTCCC AAGACCCGCT CAGCCCGAAG GCGGACCACA 
CAGATCATTG ACATCACAAT GCTGTCCAGG AAACTGGACA CAGATGAACC GGACAGTTCG 
AATTCATCCT TCTACAGCAC ACAGTCAGCC CCTGCCCCAC AGCGGGGTCT CCGTTCTGCG 
TCTTCGGCTC AGTCCCTGGC TAACTGTCCC TCTCGGGAGT CCCGGACCAG GCTGGGCTCC 
CTGGAAGATG GGAACTCAGC CTTGCTCAGC CTTCCTGGTT ATAGGCCCAA CACTCGAAGC 
TCAGCCCGGC ACTCCCAGGC CAGAGCATCT AGCGGGGCCC CTTCAGGTCG GAGCAGCTTC 
TATACAGGCA CCTGCCAGGA TGAGCCAGAG CAGCTGGATG ACTGGAACCG GATTGCAGAA 
CTTCAGCAGC GCAACCGTGT CTGCCTCCCA CACCTGAAGA CCTCTTACCC CTTGGAGGCC 
AGGCCTTCCA TGAGCCTGGC CACCATCACA GACGAAGAGA TGAAGACGGG AGACCCCCAG 
GAGACCCTTC GCCGAGCCAG CATGCAGCCA GCCCAGATCC ATGAGTTTAG TGGCACACAT 
CGCCTTCCCT CAAGCATCAC CACCCGGCAG CACCGGAAGC GCATCTCCTC GGAGCTTGAC 
CAAGGCCTCG GCACCCCTGA GGCCAAGAAA CCGACCAGCT GTTTTCCTCG TCCCCTCACC 
CCCCGGGACA GACATGAAGG CCGGAAGATG AGTGCCGCCG AGGGCCAGAA GAAGGGGGCC 
CCTGCCCCCG GCAAGCAGAC CAACAAGCGC CAGTCATTAG CCTTCAACAT TCTGAACACA 
CCCAAGAGAC TGGGGAACAG CCTGCTCCGG GGAGCCACCA GGAAATCTTC GGCTAAGACC 
TCCCCACTCC CCCGAAGTGG GACCCGCCGC TCTCCCCGAA TTGCCACTAC TACCATCACC 
ACTGGCAGCA CCTCTCCCCG GGCCAGGGGC AAGGCAAAGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007491113.1
CDS	162..6386
Translation	MTLHATRTAALLSWVNSLQIAEPVTSVLELQDCNIFIQIINRIHGTEEGQQILEQPLLDRLNFIANFLQKNCKHRSSSESLVSVQKLEDGEELELAKVIMLLLYHSTMSSKSLRDWNQFDYKIQSELAAILKFVLDHEDGFSLNDNLESFLQKAPLLSSSPSTSSDELSPMRTQQTRKEVRFLELQKIASSSGLNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERASRDELELELAKSCQLLAEKDTQISTMQQRIDRLALLNEKQATGPLESKELEELRDKNESLIVRFHEALKQCQDLKTEKGQMERKIDQLSEENGDLSFKLRDFASHLLQLQEALNDLTEEHNSSTKEWREKQVHLETELGTTLQDKKCLEEKNEILQGKISQLEEQLAQVGKNPSQEKGEVLGDILQLEALKQEVANFAAKDAELQATIKQLEDERNQWKLEMQAEQDRFKEEKQQLEQKAQTQEAQLTAQVATLHATLQQQDHDLADLKQQTEKEQAQLTETLQQQELASQGLQRQVEQLSNKLKQEEGRLEGLVQDWKVTSQTKDQQLAAAMEKYQEAQQERDAALKQLKELEKTKAVELGALQQQLQATKDACNSAQSCAAKAEQEKTILSQKVEELSTCVVELRTWQEEHKAEAKLAESLQTRVEELENQLKAEQQKLAEREELARENAQLQERLLSLEDSLQNTKGILEDEKRQAVEALEGQLRRISELEAETQSLGQQRDQDRRQLEEAKAGHQGLEAQLQKLGEEHRSKVETLQQELEKVAAARQETEGKCKQLASEAASWRTRYEENQKEGAQRGALHEEQLKALNEEQEKTQKQLQAAQERLAVLEALTKRQDAKQQEYTRLEADLSGALRQLQEKEERAQDLADSLCKLREEVDQLTAAVRKADEEKEASSQRLAEEQQRVQELEAATKQLEEQQNQKLSRIQAALQAKEQEAEQLTGKLEQARGALAESRGHQEEERVQWERERVRAEVERDQARADLATEKAAKAELEARLQSSLSEQRVEFSALQEALAHALEGAEGKEQQLGELQKQEAALRAELTKLQKTTEDLRERLAQEEAAAQADALGPAQSGAQESEALRAELGELEQRCREQEEKASELERRLEASRKAQASQENTTAALRAQLEESTQELAALRVAAQEHSKAEEEQKAQVAHAQQEAEKNSSLISSLEQEVSILNLQVLEKEGESKELKRLVLAESEKSQKLEERLRLLQTETASASTRAAECSAALSSEVEALREEAEKQRAAADGLRRELASQAEHAEGLGQELKTWRERLSQKEQSLSSLQQELATSKALVGELLPIKHLCQQLEAEKGTLQSRHREELEQGEKAVAALQAELLRARRELGDLTALRQKVTEQEQMAQQLRAENASYADQLSALQQAHSRLAEENRNLGERASHGRQQLEAELGRAQELEAVRAEAEAQAVRSREEALDTARQLETMTAKYESAKSKVLEERQRFQEERQKLTAQVEQLEAFQREQTKQVEELNKKLAQQQNMKPPESEIQQQAHHYQAQLSELQAQLSQKEQAAEHYKVQMERAKAHYDAKKQQNQELQERLQSLENLQKENAELRTESEKLGWELKQAGLKTKEAEQACRHLTAQVRTLEAQVAHADQQLRDLGKFQVATDALKTREVRGLPQLDLSTDSLELSSEEGTPLTSTRSAAHRTRKSSRTQPDGTSLPGEPASPISQRLPSKVESLESLYFTPIPARGQAPLESSMDSLGDIVLDSGPKTRSARRRTTQIIDITMLSRKLDTDEPDSSNSSFYSTQSAPAPQRGLRSASSAQSLANCPSRESRTRLGSLEDGNSALLSLPGYRPNTRSSARHSQARASSGAPSGRSSFYTGTCQDEPEQLDDWNRIAELQQRNRVCLPHLKTSYPLEARPSMSLATITDEEMKTGDPQETLRRASMQPAQIHEFSGTHRLPSSITTRQHRKRISSELDQGLGTPEAKKPTSCFPRPLTPRDRHEGRKMSAAEGQKKGAPAPGKQTNKRQSLAFNILNTPKRLGNSLLRGATRKSSAKTSPLPRSGTRRSPRIATTTITTGSTSPRARGKAKH

Target ORF information:

RefSeq Version	XM_007491051.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007491051.2

ORF Insert Sequence:

ATGACTCTTC ATGCGACCAG GACAGCTGCA CTCCTCTCTT GGGTGAACAG CCTACAGATT 
GCTGAACCTG TGACATCAGT GCTAGAGCTC CAAGACTGCA ACATCTTTAT CCAGATCATC 
AACCGCATCC ATGGCACTGA AGAGGGGCAG CAGATCCTGG AGCAGCCTCT GCTGGACAGA 
CTCAACTTCA TCGCCAACTT TCTGCAGAAA AACTGCAAAC ATCGGTCCTC CTCAGAGAGC 
CTAGTGTCTG TGCAGAAGCT AGAAGATGGG GAGGAGTTGG AGTTGGCCAA GGTGATCATG 
TTACTCTTAT ACCATTCCAC CATGAGTTCC AAAAGTTTGA GGGATTGGAA CCAATTTGAC 
TACAAAATCC AGTCGGAGTT GGCTGCCATC CTTAAATTTG TGTTGGACCA TGAAGATGGG 
TTCAGTCTGA ATGACAACTT AGAGAGTTTC CTTCAGAAAG CTCCTCTCCT TTCCAGTAGT 
CCTAGCACCA GCTCTGATGA GCTTTCCCCA ATGCGTACCC AACAAACCAG GAAGGAAGTT 
CGATTCTTGG AACTGCAGAA GATTGCCTCC TCATCAGGTC TAAACAACTT CTTGTCAGGG 
TCTCCTGCTT CTCCCATGGG TGATATCCTT CAGACCCCCC AGTTCCAAAT GAGACGGTTG 
AAGAAACAGC TGGCAGATGA GCGGGCTAGC CGAGATGAGT TGGAGCTGGA GTTAGCCAAA 
AGCTGCCAAC TACTGGCTGA GAAGGACACA CAGATTTCCA CGATGCAGCA GCGTATTGAT 
CGCTTGGCTC TCCTAAATGA GAAGCAGGCC ACAGGACCCC TGGAATCTAA AGAGCTGGAA 
GAATTACGTG ACAAGAATGA GAGTCTGATT GTACGGTTTC ATGAAGCCTT GAAACAGTGC 
CAGGACTTGA AGACTGAGAA GGGCCAGATG GAGCGCAAGA TTGACCAGCT CTCTGAGGAG 
AATGGGGACC TTTCTTTCAA GCTTCGGGAT TTTGCAAGCC ATCTGCTGCA GCTACAGGAG 
GCTCTCAATG ATCTGACAGA AGAACATAAT AGCTCCACAA AGGAATGGAG GGAGAAACAG 
GTTCACCTGG AGACAGAACT TGGCACCACC CTGCAAGACA AGAAATGCCT TGAAGAGAAG 
AATGAGATCC TCCAGGGAAA AATATCACAG TTGGAGGAGC AGCTGGCACA GGTTGGGAAG 
AATCCATCTC AAGAGAAGGG AGAGGTGTTG GGCGACATCT TGCAGCTGGA AGCCCTGAAA 
CAAGAAGTAG CCAACTTTGC TGCAAAGGAT GCTGAGCTTC AAGCCACAAT AAAGCAACTG 
GAGGATGAAC GGAATCAGTG GAAATTAGAG ATGCAAGCTG AGCAGGACCG CTTTAAGGAA 
GAGAAGCAGC AGCTGGAGCA GAAGGCTCAG ACTCAGGAAG CCCAGCTGAC TGCTCAGGTG 
GCCACCCTCC ATGCCACCCT CCAGCAGCAG GACCATGATC TGGCTGACCT CAAGCAGCAG 
ACTGAAAAGG AACAAGCACA ACTGACTGAG ACCCTTCAAC AACAAGAGCT GGCTTCCCAA 
GGCCTCCAGC GCCAGGTGGA GCAACTGAGC AACAAGCTAA AGCAAGAGGA GGGAAGGCTG 
GAGGGACTGG TCCAGGATTG GAAGGTGACC AGCCAGACCA AGGACCAACA GCTGGCAGCA 
GCTATGGAGA AGTACCAGGA GGCCCAGCAA GAGAGGGATG CAGCCCTGAA ACAGTTGAAG 
GAGTTGGAGA AAACGAAAGC TGTGGAGTTG GGAGCTCTGC AACAGCAGCT CCAAGCTACC 
AAGGATGCCT GTAACAGTGC CCAGTCTTGT GCTGCAAAGG CTGAGCAGGA GAAGACCATA 
CTGAGCCAGA AGGTGGAGGA GCTTAGCACG TGTGTAGTTG AACTGAGGAC CTGGCAAGAG 
GAGCACAAAG CAGAAGCTAA ATTGGCAGAA AGCCTTCAGA CTCGGGTCGA GGAACTGGAG 
AACCAGCTGA AGGCTGAACA ACAGAAACTA GCAGAGAGAG AAGAACTAGC TCGGGAGAAT 
GCCCAGCTCC AGGAACGACT TCTTTCCCTG GAAGATTCGT TGCAGAATAC TAAGGGCATC 
CTGGAAGATG AGAAGCGACA AGCTGTAGAG GCCCTGGAGG GGCAACTACG CCGCATTTCT 
GAGCTGGAGG CAGAGACGCA GAGCCTTGGG CAGCAGCGAG ACCAAGATCG GCGGCAGCTG 
GAGGAGGCAA AGGCTGGGCA TCAGGGTCTG GAGGCCCAGC TCCAGAAGCT TGGAGAAGAG 
CATCGTTCCA AAGTAGAAAC CTTGCAGCAG GAACTGGAAA AGGTGGCAGC TGCCCGGCAA 
GAGACAGAGG GAAAGTGCAA GCAGCTGGCC TCAGAGGCAG CTTCCTGGCG TACTCGTTAT 
GAAGAGAACC AGAAGGAGGG GGCGCAGCGT GGTGCCCTGC ATGAGGAACA GTTGAAGGCC 
CTGAATGAGG AGCAAGAGAA AACCCAAAAG CAGCTGCAGG CTGCCCAGGA GAGATTGGCA 
GTGCTGGAGG CTCTCACCAA ACGCCAGGAT GCTAAACAGC AAGAATACAC TCGGCTTGAG 
GCTGACCTGT CAGGTGCCCT CCGGCAGCTC CAGGAGAAGG AAGAGCGGGC CCAGGATCTG 
GCCGACAGCC TGTGCAAGCT GCGGGAGGAG GTAGATCAGC TCACAGCTGC AGTCAGGAAA 
GCAGATGAGG AGAAGGAAGC CTCTTCTCAG AGGCTGGCTG AAGAGCAGCA ACGGGTGCAA 
GAGCTTGAGG CTGCCACAAA GCAGCTGGAA GAGCAGCAGA ACCAGAAACT AAGCAGGATC 
CAGGCGGCCC TGCAGGCTAA GGAGCAAGAA GCAGAGCAGC TGACAGGAAA GCTAGAGCAA 
GCACGCGGTG CTCTGGCAGA GAGCCGGGGG CACCAGGAGG AAGAGCGTGT CCAGTGGGAG 
CGGGAAAGAG TCCGTGCAGA GGTGGAACGA GACCAGGCCA GAGCCGACCT GGCCACTGAG 
AAGGCTGCTA AGGCAGAACT GGAGGCAAGG CTGCAGAGCT CCCTGAGTGA GCAGCGGGTG 
GAGTTCAGTG CCCTGCAGGA AGCCTTGGCC CATGCCCTGG AAGGAGCAGA AGGGAAGGAA 
CAGCAGCTTG GAGAGCTTCA GAAGCAAGAG GCCGCCCTGC GGGCAGAACT GACTAAGCTG 
CAGAAGACCA CTGAGGACTT GAGAGAAAGG TTGGCTCAGG AGGAGGCAGC TGCCCAAGCA 
GATGCTCTGG GGCCTGCACA GTCGGGCGCC CAGGAGTCAG AGGCTCTGCG GGCCGAGCTG 
GGTGAGCTGG AGCAGCGGTG CCGGGAGCAG GAGGAGAAGG CCTCTGAGCT GGAGCGCCGC 
CTTGAGGCTT CTCGGAAGGC CCAGGCCAGT CAGGAAAACA CTACTGCTGC CCTCCGGGCT 
CAGCTGGAGG AAAGCACCCA AGAGCTAGCT GCCCTCCGAG TGGCAGCTCA GGAGCACAGC 
AAGGCTGAAG AGGAGCAAAA AGCCCAGGTG GCCCATGCCC AGCAGGAGGC AGAGAAGAAT 
AGCAGCCTCA TCAGCAGCTT GGAGCAGGAG GTGTCCATCT TGAACCTACA GGTTCTGGAG 
AAAGAAGGAG AAAGCAAAGA GCTGAAGCGT CTAGTGCTGG CTGAGTCAGA GAAAAGCCAG 
AAGCTGGAGG AGCGCCTGCG CCTGCTTCAG ACAGAAACTG CCAGTGCCAG CACACGGGCG 
GCAGAGTGCA GCGCAGCCCT GAGCTCTGAG GTGGAGGCGC TCCGGGAAGA GGCTGAAAAG 
CAGCGAGCGG CTGCAGATGG TCTCCGGAGG GAGCTGGCCT CCCAGGCTGA GCATGCAGAG 
GGCCTGGGAC AGGAACTGAA GACTTGGCGT GAGAGGCTAT CCCAGAAGGA GCAGTCCCTG 
TCCTCACTGC AGCAAGAGCT TGCCACCTCC AAGGCCCTGG TTGGGGAACT GTTGCCCATC 
AAGCACCTCT GCCAGCAGTT AGAGGCCGAG AAGGGCACCT TGCAGAGCCG GCACCGAGAG 
GAGCTTGAGC AGGGTGAGAA GGCTGTGGCG GCGCTGCAGG CAGAGCTTCT TCGGGCCCGC 
CGAGAGCTTG GAGACCTCAC TGCATTGCGA CAGAAAGTGA CTGAGCAGGA GCAGATGGCT 
CAGCAGCTGA GGGCAGAGAA TGCCAGCTAT GCTGACCAAC TGAGTGCACT ACAACAGGCC 
CATAGCAGGC TAGCAGAGGA GAACCGAAAC CTGGGGGAGC GGGCCAGCCA CGGGCGGCAG 
CAGCTGGAGG CTGAGCTTGG ACGAGCCCAG GAACTTGAGG CAGTGCGGGC TGAGGCAGAA 
GCCCAGGCAG TTCGGAGCCG GGAGGAGGCC CTAGATACAG CCAGGCAGCT GGAGACCATG 
ACGGCCAAGT ATGAGAGTGC CAAGAGCAAG GTCTTGGAGG AGAGGCAGAG GTTCCAGGAA 
GAGCGGCAGA AACTCACTGC CCAGGTGGAG CAACTAGAGG CATTTCAGAG AGAACAGACT 
AAACAGGTGG AGGAACTGAA TAAGAAGCTG GCCCAGCAAC AAAATATGAA GCCCCCTGAA 
AGTGAAATAC AGCAGCAAGC TCATCATTAC CAGGCCCAAC TCAGTGAGCT GCAGGCCCAA 
CTGAGCCAGA AGGAACAGGC TGCTGAGCAC TATAAGGTGC AGATGGAAAG AGCCAAGGCT 
CACTATGATG CCAAGAAGCA GCAGAACCAG GAGCTGCAGG AACGTCTGCA GAGCTTGGAG 
AATCTACAGA AGGAGAATGC TGAACTGCGG ACCGAGTCTG AGAAGCTCGG CTGGGAACTG 
AAGCAAGCTG GGCTGAAGAC CAAGGAAGCT GAGCAAGCCT GCCGCCATCT TACTGCCCAG 
GTTCGAACTC TGGAGGCACA GGTTGCTCAT GCAGATCAGC AGCTCCGAGA TCTTGGCAAG 
TTTCAAGTGG CAACAGATGC ACTGAAAACC CGAGAAGTCC GAGGGCTGCC CCAACTGGAT 
CTGAGCACTG ACAGCCTAGA ATTGAGCAGT GAGGAAGGGA CCCCACTGAC CTCTACAAGA 
TCAGCAGCTC ACCGTACTCG AAAGTCATCT CGAACCCAGC CTGATGGCAC CAGCCTCCCT 
GGGGAGCCAG CTTCACCCAT CTCTCAGCGC CTGCCTTCTA AGGTGGAGTC CCTGGAGAGC 
TTGTACTTCA CCCCAATACC TGCCCGAGGA CAGGCCCCCT TAGAAAGCAG CATGGACTCA 
CTGGGTGACA TTGTATTGGA TTCTGGTCCC AAGACCCGCT CAGCCCGAAG GCGGACCACA 
CAGATCATTG ACATCACAAT GCTGTCCAGG AAACTGGACA CAGATGAACC GGACAGTTCG 
AATTCATCCT TCTACAGCAC ACAGTCAGCC CCTGCCCCAC AGCGGGGTCT CCGTTCTGCG 
TCTTCGGCTC AGTCCCTGGC TAACTGTCCC TCTCGGGAGT CCCGGACCAG GCTGGGCTCC 
CTGGAAGATG GGAACTCAGC CTTGCTCAGC CTTCCTGGTT ATAGGCCCAA CACTCGAAGC 
TCAGCCCGGC ACTCCCAGGC CAGAGCATCT AGCGGGGCCC CTTCAGGTCG GAGCAGCTTC 
TATACAGGCA CCTGCCAGGA TGAGCCAGAG CAGCTGGATG ACTGGAACCG GATTGCAGAA 
CTTCAGCAGC GCAACCGTGT CTGCCTCCCA CACCTGAAGA CCTCTTACCC CTTGGAGGCC 
AGGCCTTCCA TGAGCCTGGC CACCATCACA GACGAAGAGA TGAAGACGGG AGACCCCCAG 
GAGACCCTTC GCCGAGCCAG CATGCAGCCA GCCCAGATCC ATGAGTTTAG TGGCACACAT 
CGCCTTCCCT CAAGCATCAC CACCCGGCAG CACCGGAAGC GCATCTCCTC GGAGCTTGAC 
CAAGGCCTCG GCACCCCTGA GGCCAAGAAA CCGACCAGCT GTTTTCCTCG TCCCCTCACC 
CCCCGGGACA GACATGAAGG CCGGAAGATG AGTGCCGCCG AGGGCCAGAA GAAGGGGGCC 
CCTGCCCCCG GCAAGCAGAC CAACAAGCGC CAGTCATTAG CCTTCAACAT TCTGAACACA 
CCCAAGAGAC TGGGGAACAG CCTGCTCCGG GGAGCCACCA GGAAATCTTC GGCTAAGACC 
TCCCCACTCC CCCGAAGTGG GACCCGCCGC TCTCCCCGAA TTGCCACTAC TACCATCACC 
ACTGGCAGCA CCTCTCCCCG GGCCAGGGGC AAGGCAAAGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_007491053.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007491115.1 Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X8, mRNA.

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CloneID	OMc22045
Clone ID Related Accession (Same CDS sequence)	XM_007491050.1 , XM_007491051.2 , XM_007491053.2 , XM_007491052.1
Accession Version	XM_007491053.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	nuclear mitotic apparatus protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007491053.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCTTC ATGCGACCAG GACAGCTGCA CTCCTCTCTT GGGTGAACAG CCTACAGATT 
GCTGAACCTG TGACATCAGT GCTAGAGCTC CAAGACTGCA ACATCTTTAT CCAGATCATC 
AACCGCATCC ATGGCACTGA AGAGGGGCAG CAGATCCTGG AGCAGCCTCT GCTGGACAGA 
CTCAACTTCA TCGCCAACTT TCTGCAGAAA AACTGCAAAC ATCGGTCCTC CTCAGAGAGC 
CTAGTGTCTG TGCAGAAGCT AGAAGATGGG GAGGAGTTGG AGTTGGCCAA GGTGATCATG 
TTACTCTTAT ACCATTCCAC CATGAGTTCC AAAAGTTTGA GGGATTGGAA CCAATTTGAC 
TACAAAATCC AGTCGGAGTT GGCTGCCATC CTTAAATTTG TGTTGGACCA TGAAGATGGG 
TTCAGTCTGA ATGACAACTT AGAGAGTTTC CTTCAGAAAG CTCCTCTCCT TTCCAGTAGT 
CCTAGCACCA GCTCTGATGA GCTTTCCCCA ATGCGTACCC AACAAACCAG GAAGGAAGTT 
CGATTCTTGG AACTGCAGAA GATTGCCTCC TCATCAGGTC TAAACAACTT CTTGTCAGGG 
TCTCCTGCTT CTCCCATGGG TGATATCCTT CAGACCCCCC AGTTCCAAAT GAGACGGTTG 
AAGAAACAGC TGGCAGATGA GCGGGCTAGC CGAGATGAGT TGGAGCTGGA GTTAGCCAAA 
AGCTGCCAAC TACTGGCTGA GAAGGACACA CAGATTTCCA CGATGCAGCA GCGTATTGAT 
CGCTTGGCTC TCCTAAATGA GAAGCAGGCC ACAGGACCCC TGGAATCTAA AGAGCTGGAA 
GAATTACGTG ACAAGAATGA GAGTCTGATT GTACGGTTTC ATGAAGCCTT GAAACAGTGC 
CAGGACTTGA AGACTGAGAA GGGCCAGATG GAGCGCAAGA TTGACCAGCT CTCTGAGGAG 
AATGGGGACC TTTCTTTCAA GCTTCGGGAT TTTGCAAGCC ATCTGCTGCA GCTACAGGAG 
GCTCTCAATG ATCTGACAGA AGAACATAAT AGCTCCACAA AGGAATGGAG GGAGAAACAG 
GTTCACCTGG AGACAGAACT TGGCACCACC CTGCAAGACA AGAAATGCCT TGAAGAGAAG 
AATGAGATCC TCCAGGGAAA AATATCACAG TTGGAGGAGC AGCTGGCACA GGTTGGGAAG 
AATCCATCTC AAGAGAAGGG AGAGGTGTTG GGCGACATCT TGCAGCTGGA AGCCCTGAAA 
CAAGAAGTAG CCAACTTTGC TGCAAAGGAT GCTGAGCTTC AAGCCACAAT AAAGCAACTG 
GAGGATGAAC GGAATCAGTG GAAATTAGAG ATGCAAGCTG AGCAGGACCG CTTTAAGGAA 
GAGAAGCAGC AGCTGGAGCA GAAGGCTCAG ACTCAGGAAG CCCAGCTGAC TGCTCAGGTG 
GCCACCCTCC ATGCCACCCT CCAGCAGCAG GACCATGATC TGGCTGACCT CAAGCAGCAG 
ACTGAAAAGG AACAAGCACA ACTGACTGAG ACCCTTCAAC AACAAGAGCT GGCTTCCCAA 
GGCCTCCAGC GCCAGGTGGA GCAACTGAGC AACAAGCTAA AGCAAGAGGA GGGAAGGCTG 
GAGGGACTGG TCCAGGATTG GAAGGTGACC AGCCAGACCA AGGACCAACA GCTGGCAGCA 
GCTATGGAGA AGTACCAGGA GGCCCAGCAA GAGAGGGATG CAGCCCTGAA ACAGTTGAAG 
GAGTTGGAGA AAACGAAAGC TGTGGAGTTG GGAGCTCTGC AACAGCAGCT CCAAGCTACC 
AAGGATGCCT GTAACAGTGC CCAGTCTTGT GCTGCAAAGG CTGAGCAGGA GAAGACCATA 
CTGAGCCAGA AGGTGGAGGA GCTTAGCACG TGTGTAGTTG AACTGAGGAC CTGGCAAGAG 
GAGCACAAAG CAGAAGCTAA ATTGGCAGAA AGCCTTCAGA CTCGGGTCGA GGAACTGGAG 
AACCAGCTGA AGGCTGAACA ACAGAAACTA GCAGAGAGAG AAGAACTAGC TCGGGAGAAT 
GCCCAGCTCC AGGAACGACT TCTTTCCCTG GAAGATTCGT TGCAGAATAC TAAGGGCATC 
CTGGAAGATG AGAAGCGACA AGCTGTAGAG GCCCTGGAGG GGCAACTACG CCGCATTTCT 
GAGCTGGAGG CAGAGACGCA GAGCCTTGGG CAGCAGCGAG ACCAAGATCG GCGGCAGCTG 
GAGGAGGCAA AGGCTGGGCA TCAGGGTCTG GAGGCCCAGC TCCAGAAGCT TGGAGAAGAG 
CATCGTTCCA AAGTAGAAAC CTTGCAGCAG GAACTGGAAA AGGTGGCAGC TGCCCGGCAA 
GAGACAGAGG GAAAGTGCAA GCAGCTGGCC TCAGAGGCAG CTTCCTGGCG TACTCGTTAT 
GAAGAGAACC AGAAGGAGGG GGCGCAGCGT GGTGCCCTGC ATGAGGAACA GTTGAAGGCC 
CTGAATGAGG AGCAAGAGAA AACCCAAAAG CAGCTGCAGG CTGCCCAGGA GAGATTGGCA 
GTGCTGGAGG CTCTCACCAA ACGCCAGGAT GCTAAACAGC AAGAATACAC TCGGCTTGAG 
GCTGACCTGT CAGGTGCCCT CCGGCAGCTC CAGGAGAAGG AAGAGCGGGC CCAGGATCTG 
GCCGACAGCC TGTGCAAGCT GCGGGAGGAG GTAGATCAGC TCACAGCTGC AGTCAGGAAA 
GCAGATGAGG AGAAGGAAGC CTCTTCTCAG AGGCTGGCTG AAGAGCAGCA ACGGGTGCAA 
GAGCTTGAGG CTGCCACAAA GCAGCTGGAA GAGCAGCAGA ACCAGAAACT AAGCAGGATC 
CAGGCGGCCC TGCAGGCTAA GGAGCAAGAA GCAGAGCAGC TGACAGGAAA GCTAGAGCAA 
GCACGCGGTG CTCTGGCAGA GAGCCGGGGG CACCAGGAGG AAGAGCGTGT CCAGTGGGAG 
CGGGAAAGAG TCCGTGCAGA GGTGGAACGA GACCAGGCCA GAGCCGACCT GGCCACTGAG 
AAGGCTGCTA AGGCAGAACT GGAGGCAAGG CTGCAGAGCT CCCTGAGTGA GCAGCGGGTG 
GAGTTCAGTG CCCTGCAGGA AGCCTTGGCC CATGCCCTGG AAGGAGCAGA AGGGAAGGAA 
CAGCAGCTTG GAGAGCTTCA GAAGCAAGAG GCCGCCCTGC GGGCAGAACT GACTAAGCTG 
CAGAAGACCA CTGAGGACTT GAGAGAAAGG TTGGCTCAGG AGGAGGCAGC TGCCCAAGCA 
GATGCTCTGG GGCCTGCACA GTCGGGCGCC CAGGAGTCAG AGGCTCTGCG GGCCGAGCTG 
GGTGAGCTGG AGCAGCGGTG CCGGGAGCAG GAGGAGAAGG CCTCTGAGCT GGAGCGCCGC 
CTTGAGGCTT CTCGGAAGGC CCAGGCCAGT CAGGAAAACA CTACTGCTGC CCTCCGGGCT 
CAGCTGGAGG AAAGCACCCA AGAGCTAGCT GCCCTCCGAG TGGCAGCTCA GGAGCACAGC 
AAGGCTGAAG AGGAGCAAAA AGCCCAGGTG GCCCATGCCC AGCAGGAGGC AGAGAAGAAT 
AGCAGCCTCA TCAGCAGCTT GGAGCAGGAG GTGTCCATCT TGAACCTACA GGTTCTGGAG 
AAAGAAGGAG AAAGCAAAGA GCTGAAGCGT CTAGTGCTGG CTGAGTCAGA GAAAAGCCAG 
AAGCTGGAGG AGCGCCTGCG CCTGCTTCAG ACAGAAACTG CCAGTGCCAG CACACGGGCG 
GCAGAGTGCA GCGCAGCCCT GAGCTCTGAG GTGGAGGCGC TCCGGGAAGA GGCTGAAAAG 
CAGCGAGCGG CTGCAGATGG TCTCCGGAGG GAGCTGGCCT CCCAGGCTGA GCATGCAGAG 
GGCCTGGGAC AGGAACTGAA GACTTGGCGT GAGAGGCTAT CCCAGAAGGA GCAGTCCCTG 
TCCTCACTGC AGCAAGAGCT TGCCACCTCC AAGGCCCTGG TTGGGGAACT GTTGCCCATC 
AAGCACCTCT GCCAGCAGTT AGAGGCCGAG AAGGGCACCT TGCAGAGCCG GCACCGAGAG 
GAGCTTGAGC AGGGTGAGAA GGCTGTGGCG GCGCTGCAGG CAGAGCTTCT TCGGGCCCGC 
CGAGAGCTTG GAGACCTCAC TGCATTGCGA CAGAAAGTGA CTGAGCAGGA GCAGATGGCT 
CAGCAGCTGA GGGCAGAGAA TGCCAGCTAT GCTGACCAAC TGAGTGCACT ACAACAGGCC 
CATAGCAGGC TAGCAGAGGA GAACCGAAAC CTGGGGGAGC GGGCCAGCCA CGGGCGGCAG 
CAGCTGGAGG CTGAGCTTGG ACGAGCCCAG GAACTTGAGG CAGTGCGGGC TGAGGCAGAA 
GCCCAGGCAG TTCGGAGCCG GGAGGAGGCC CTAGATACAG CCAGGCAGCT GGAGACCATG 
ACGGCCAAGT ATGAGAGTGC CAAGAGCAAG GTCTTGGAGG AGAGGCAGAG GTTCCAGGAA 
GAGCGGCAGA AACTCACTGC CCAGGTGGAG CAACTAGAGG CATTTCAGAG AGAACAGACT 
AAACAGGTGG AGGAACTGAA TAAGAAGCTG GCCCAGCAAC AAAATATGAA GCCCCCTGAA 
AGTGAAATAC AGCAGCAAGC TCATCATTAC CAGGCCCAAC TCAGTGAGCT GCAGGCCCAA 
CTGAGCCAGA AGGAACAGGC TGCTGAGCAC TATAAGGTGC AGATGGAAAG AGCCAAGGCT 
CACTATGATG CCAAGAAGCA GCAGAACCAG GAGCTGCAGG AACGTCTGCA GAGCTTGGAG 
AATCTACAGA AGGAGAATGC TGAACTGCGG ACCGAGTCTG AGAAGCTCGG CTGGGAACTG 
AAGCAAGCTG GGCTGAAGAC CAAGGAAGCT GAGCAAGCCT GCCGCCATCT TACTGCCCAG 
GTTCGAACTC TGGAGGCACA GGTTGCTCAT GCAGATCAGC AGCTCCGAGA TCTTGGCAAG 
TTTCAAGTGG CAACAGATGC ACTGAAAACC CGAGAAGTCC GAGGGCTGCC CCAACTGGAT 
CTGAGCACTG ACAGCCTAGA ATTGAGCAGT GAGGAAGGGA CCCCACTGAC CTCTACAAGA 
TCAGCAGCTC ACCGTACTCG AAAGTCATCT CGAACCCAGC CTGATGGCAC CAGCCTCCCT 
GGGGAGCCAG CTTCACCCAT CTCTCAGCGC CTGCCTTCTA AGGTGGAGTC CCTGGAGAGC 
TTGTACTTCA CCCCAATACC TGCCCGAGGA CAGGCCCCCT TAGAAAGCAG CATGGACTCA 
CTGGGTGACA TTGTATTGGA TTCTGGTCCC AAGACCCGCT CAGCCCGAAG GCGGACCACA 
CAGATCATTG ACATCACAAT GCTGTCCAGG AAACTGGACA CAGATGAACC GGACAGTTCG 
AATTCATCCT TCTACAGCAC ACAGTCAGCC CCTGCCCCAC AGCGGGGTCT CCGTTCTGCG 
TCTTCGGCTC AGTCCCTGGC TAACTGTCCC TCTCGGGAGT CCCGGACCAG GCTGGGCTCC 
CTGGAAGATG GGAACTCAGC CTTGCTCAGC CTTCCTGGTT ATAGGCCCAA CACTCGAAGC 
TCAGCCCGGC ACTCCCAGGC CAGAGCATCT AGCGGGGCCC CTTCAGGTCG GAGCAGCTTC 
TATACAGGCA CCTGCCAGGA TGAGCCAGAG CAGCTGGATG ACTGGAACCG GATTGCAGAA 
CTTCAGCAGC GCAACCGTGT CTGCCTCCCA CACCTGAAGA CCTCTTACCC CTTGGAGGCC 
AGGCCTTCCA TGAGCCTGGC CACCATCACA GACGAAGAGA TGAAGACGGG AGACCCCCAG 
GAGACCCTTC GCCGAGCCAG CATGCAGCCA GCCCAGATCC ATGAGTTTAG TGGCACACAT 
CGCCTTCCCT CAAGCATCAC CACCCGGCAG CACCGGAAGC GCATCTCCTC GGAGCTTGAC 
CAAGGCCTCG GCACCCCTGA GGCCAAGAAA CCGACCAGCT GTTTTCCTCG TCCCCTCACC 
CCCCGGGACA GACATGAAGG CCGGAAGATG AGTGCCGCCG AGGGCCAGAA GAAGGGGGCC 
CCTGCCCCCG GCAAGCAGAC CAACAAGCGC CAGTCATTAG CCTTCAACAT TCTGAACACA 
CCCAAGAGAC TGGGGAACAG CCTGCTCCGG GGAGCCACCA GGAAATCTTC GGCTAAGACC 
TCCCCACTCC CCCGAAGTGG GACCCGCCGC TCTCCCCGAA TTGCCACTAC TACCATCACC 
ACTGGCAGCA CCTCTCCCCG GGCCAGGGGC AAGGCAAAGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007491115.1
CDS	167..6391
Translation	MTLHATRTAALLSWVNSLQIAEPVTSVLELQDCNIFIQIINRIHGTEEGQQILEQPLLDRLNFIANFLQKNCKHRSSSESLVSVQKLEDGEELELAKVIMLLLYHSTMSSKSLRDWNQFDYKIQSELAAILKFVLDHEDGFSLNDNLESFLQKAPLLSSSPSTSSDELSPMRTQQTRKEVRFLELQKIASSSGLNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERASRDELELELAKSCQLLAEKDTQISTMQQRIDRLALLNEKQATGPLESKELEELRDKNESLIVRFHEALKQCQDLKTEKGQMERKIDQLSEENGDLSFKLRDFASHLLQLQEALNDLTEEHNSSTKEWREKQVHLETELGTTLQDKKCLEEKNEILQGKISQLEEQLAQVGKNPSQEKGEVLGDILQLEALKQEVANFAAKDAELQATIKQLEDERNQWKLEMQAEQDRFKEEKQQLEQKAQTQEAQLTAQVATLHATLQQQDHDLADLKQQTEKEQAQLTETLQQQELASQGLQRQVEQLSNKLKQEEGRLEGLVQDWKVTSQTKDQQLAAAMEKYQEAQQERDAALKQLKELEKTKAVELGALQQQLQATKDACNSAQSCAAKAEQEKTILSQKVEELSTCVVELRTWQEEHKAEAKLAESLQTRVEELENQLKAEQQKLAEREELARENAQLQERLLSLEDSLQNTKGILEDEKRQAVEALEGQLRRISELEAETQSLGQQRDQDRRQLEEAKAGHQGLEAQLQKLGEEHRSKVETLQQELEKVAAARQETEGKCKQLASEAASWRTRYEENQKEGAQRGALHEEQLKALNEEQEKTQKQLQAAQERLAVLEALTKRQDAKQQEYTRLEADLSGALRQLQEKEERAQDLADSLCKLREEVDQLTAAVRKADEEKEASSQRLAEEQQRVQELEAATKQLEEQQNQKLSRIQAALQAKEQEAEQLTGKLEQARGALAESRGHQEEERVQWERERVRAEVERDQARADLATEKAAKAELEARLQSSLSEQRVEFSALQEALAHALEGAEGKEQQLGELQKQEAALRAELTKLQKTTEDLRERLAQEEAAAQADALGPAQSGAQESEALRAELGELEQRCREQEEKASELERRLEASRKAQASQENTTAALRAQLEESTQELAALRVAAQEHSKAEEEQKAQVAHAQQEAEKNSSLISSLEQEVSILNLQVLEKEGESKELKRLVLAESEKSQKLEERLRLLQTETASASTRAAECSAALSSEVEALREEAEKQRAAADGLRRELASQAEHAEGLGQELKTWRERLSQKEQSLSSLQQELATSKALVGELLPIKHLCQQLEAEKGTLQSRHREELEQGEKAVAALQAELLRARRELGDLTALRQKVTEQEQMAQQLRAENASYADQLSALQQAHSRLAEENRNLGERASHGRQQLEAELGRAQELEAVRAEAEAQAVRSREEALDTARQLETMTAKYESAKSKVLEERQRFQEERQKLTAQVEQLEAFQREQTKQVEELNKKLAQQQNMKPPESEIQQQAHHYQAQLSELQAQLSQKEQAAEHYKVQMERAKAHYDAKKQQNQELQERLQSLENLQKENAELRTESEKLGWELKQAGLKTKEAEQACRHLTAQVRTLEAQVAHADQQLRDLGKFQVATDALKTREVRGLPQLDLSTDSLELSSEEGTPLTSTRSAAHRTRKSSRTQPDGTSLPGEPASPISQRLPSKVESLESLYFTPIPARGQAPLESSMDSLGDIVLDSGPKTRSARRRTTQIIDITMLSRKLDTDEPDSSNSSFYSTQSAPAPQRGLRSASSAQSLANCPSRESRTRLGSLEDGNSALLSLPGYRPNTRSSARHSQARASSGAPSGRSSFYTGTCQDEPEQLDDWNRIAELQQRNRVCLPHLKTSYPLEARPSMSLATITDEEMKTGDPQETLRRASMQPAQIHEFSGTHRLPSSITTRQHRKRISSELDQGLGTPEAKKPTSCFPRPLTPRDRHEGRKMSAAEGQKKGAPAPGKQTNKRQSLAFNILNTPKRLGNSLLRGATRKSSAKTSPLPRSGTRRSPRIATTTITTGSTSPRARGKAKH

Target ORF information:

RefSeq Version	XM_007491053.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007491053.2

ORF Insert Sequence:

ATGACTCTTC ATGCGACCAG GACAGCTGCA CTCCTCTCTT GGGTGAACAG CCTACAGATT 
GCTGAACCTG TGACATCAGT GCTAGAGCTC CAAGACTGCA ACATCTTTAT CCAGATCATC 
AACCGCATCC ATGGCACTGA AGAGGGGCAG CAGATCCTGG AGCAGCCTCT GCTGGACAGA 
CTCAACTTCA TCGCCAACTT TCTGCAGAAA AACTGCAAAC ATCGGTCCTC CTCAGAGAGC 
CTAGTGTCTG TGCAGAAGCT AGAAGATGGG GAGGAGTTGG AGTTGGCCAA GGTGATCATG 
TTACTCTTAT ACCATTCCAC CATGAGTTCC AAAAGTTTGA GGGATTGGAA CCAATTTGAC 
TACAAAATCC AGTCGGAGTT GGCTGCCATC CTTAAATTTG TGTTGGACCA TGAAGATGGG 
TTCAGTCTGA ATGACAACTT AGAGAGTTTC CTTCAGAAAG CTCCTCTCCT TTCCAGTAGT 
CCTAGCACCA GCTCTGATGA GCTTTCCCCA ATGCGTACCC AACAAACCAG GAAGGAAGTT 
CGATTCTTGG AACTGCAGAA GATTGCCTCC TCATCAGGTC TAAACAACTT CTTGTCAGGG 
TCTCCTGCTT CTCCCATGGG TGATATCCTT CAGACCCCCC AGTTCCAAAT GAGACGGTTG 
AAGAAACAGC TGGCAGATGA GCGGGCTAGC CGAGATGAGT TGGAGCTGGA GTTAGCCAAA 
AGCTGCCAAC TACTGGCTGA GAAGGACACA CAGATTTCCA CGATGCAGCA GCGTATTGAT 
CGCTTGGCTC TCCTAAATGA GAAGCAGGCC ACAGGACCCC TGGAATCTAA AGAGCTGGAA 
GAATTACGTG ACAAGAATGA GAGTCTGATT GTACGGTTTC ATGAAGCCTT GAAACAGTGC 
CAGGACTTGA AGACTGAGAA GGGCCAGATG GAGCGCAAGA TTGACCAGCT CTCTGAGGAG 
AATGGGGACC TTTCTTTCAA GCTTCGGGAT TTTGCAAGCC ATCTGCTGCA GCTACAGGAG 
GCTCTCAATG ATCTGACAGA AGAACATAAT AGCTCCACAA AGGAATGGAG GGAGAAACAG 
GTTCACCTGG AGACAGAACT TGGCACCACC CTGCAAGACA AGAAATGCCT TGAAGAGAAG 
AATGAGATCC TCCAGGGAAA AATATCACAG TTGGAGGAGC AGCTGGCACA GGTTGGGAAG 
AATCCATCTC AAGAGAAGGG AGAGGTGTTG GGCGACATCT TGCAGCTGGA AGCCCTGAAA 
CAAGAAGTAG CCAACTTTGC TGCAAAGGAT GCTGAGCTTC AAGCCACAAT AAAGCAACTG 
GAGGATGAAC GGAATCAGTG GAAATTAGAG ATGCAAGCTG AGCAGGACCG CTTTAAGGAA 
GAGAAGCAGC AGCTGGAGCA GAAGGCTCAG ACTCAGGAAG CCCAGCTGAC TGCTCAGGTG 
GCCACCCTCC ATGCCACCCT CCAGCAGCAG GACCATGATC TGGCTGACCT CAAGCAGCAG 
ACTGAAAAGG AACAAGCACA ACTGACTGAG ACCCTTCAAC AACAAGAGCT GGCTTCCCAA 
GGCCTCCAGC GCCAGGTGGA GCAACTGAGC AACAAGCTAA AGCAAGAGGA GGGAAGGCTG 
GAGGGACTGG TCCAGGATTG GAAGGTGACC AGCCAGACCA AGGACCAACA GCTGGCAGCA 
GCTATGGAGA AGTACCAGGA GGCCCAGCAA GAGAGGGATG CAGCCCTGAA ACAGTTGAAG 
GAGTTGGAGA AAACGAAAGC TGTGGAGTTG GGAGCTCTGC AACAGCAGCT CCAAGCTACC 
AAGGATGCCT GTAACAGTGC CCAGTCTTGT GCTGCAAAGG CTGAGCAGGA GAAGACCATA 
CTGAGCCAGA AGGTGGAGGA GCTTAGCACG TGTGTAGTTG AACTGAGGAC CTGGCAAGAG 
GAGCACAAAG CAGAAGCTAA ATTGGCAGAA AGCCTTCAGA CTCGGGTCGA GGAACTGGAG 
AACCAGCTGA AGGCTGAACA ACAGAAACTA GCAGAGAGAG AAGAACTAGC TCGGGAGAAT 
GCCCAGCTCC AGGAACGACT TCTTTCCCTG GAAGATTCGT TGCAGAATAC TAAGGGCATC 
CTGGAAGATG AGAAGCGACA AGCTGTAGAG GCCCTGGAGG GGCAACTACG CCGCATTTCT 
GAGCTGGAGG CAGAGACGCA GAGCCTTGGG CAGCAGCGAG ACCAAGATCG GCGGCAGCTG 
GAGGAGGCAA AGGCTGGGCA TCAGGGTCTG GAGGCCCAGC TCCAGAAGCT TGGAGAAGAG 
CATCGTTCCA AAGTAGAAAC CTTGCAGCAG GAACTGGAAA AGGTGGCAGC TGCCCGGCAA 
GAGACAGAGG GAAAGTGCAA GCAGCTGGCC TCAGAGGCAG CTTCCTGGCG TACTCGTTAT 
GAAGAGAACC AGAAGGAGGG GGCGCAGCGT GGTGCCCTGC ATGAGGAACA GTTGAAGGCC 
CTGAATGAGG AGCAAGAGAA AACCCAAAAG CAGCTGCAGG CTGCCCAGGA GAGATTGGCA 
GTGCTGGAGG CTCTCACCAA ACGCCAGGAT GCTAAACAGC AAGAATACAC TCGGCTTGAG 
GCTGACCTGT CAGGTGCCCT CCGGCAGCTC CAGGAGAAGG AAGAGCGGGC CCAGGATCTG 
GCCGACAGCC TGTGCAAGCT GCGGGAGGAG GTAGATCAGC TCACAGCTGC AGTCAGGAAA 
GCAGATGAGG AGAAGGAAGC CTCTTCTCAG AGGCTGGCTG AAGAGCAGCA ACGGGTGCAA 
GAGCTTGAGG CTGCCACAAA GCAGCTGGAA GAGCAGCAGA ACCAGAAACT AAGCAGGATC 
CAGGCGGCCC TGCAGGCTAA GGAGCAAGAA GCAGAGCAGC TGACAGGAAA GCTAGAGCAA 
GCACGCGGTG CTCTGGCAGA GAGCCGGGGG CACCAGGAGG AAGAGCGTGT CCAGTGGGAG 
CGGGAAAGAG TCCGTGCAGA GGTGGAACGA GACCAGGCCA GAGCCGACCT GGCCACTGAG 
AAGGCTGCTA AGGCAGAACT GGAGGCAAGG CTGCAGAGCT CCCTGAGTGA GCAGCGGGTG 
GAGTTCAGTG CCCTGCAGGA AGCCTTGGCC CATGCCCTGG AAGGAGCAGA AGGGAAGGAA 
CAGCAGCTTG GAGAGCTTCA GAAGCAAGAG GCCGCCCTGC GGGCAGAACT GACTAAGCTG 
CAGAAGACCA CTGAGGACTT GAGAGAAAGG TTGGCTCAGG AGGAGGCAGC TGCCCAAGCA 
GATGCTCTGG GGCCTGCACA GTCGGGCGCC CAGGAGTCAG AGGCTCTGCG GGCCGAGCTG 
GGTGAGCTGG AGCAGCGGTG CCGGGAGCAG GAGGAGAAGG CCTCTGAGCT GGAGCGCCGC 
CTTGAGGCTT CTCGGAAGGC CCAGGCCAGT CAGGAAAACA CTACTGCTGC CCTCCGGGCT 
CAGCTGGAGG AAAGCACCCA AGAGCTAGCT GCCCTCCGAG TGGCAGCTCA GGAGCACAGC 
AAGGCTGAAG AGGAGCAAAA AGCCCAGGTG GCCCATGCCC AGCAGGAGGC AGAGAAGAAT 
AGCAGCCTCA TCAGCAGCTT GGAGCAGGAG GTGTCCATCT TGAACCTACA GGTTCTGGAG 
AAAGAAGGAG AAAGCAAAGA GCTGAAGCGT CTAGTGCTGG CTGAGTCAGA GAAAAGCCAG 
AAGCTGGAGG AGCGCCTGCG CCTGCTTCAG ACAGAAACTG CCAGTGCCAG CACACGGGCG 
GCAGAGTGCA GCGCAGCCCT GAGCTCTGAG GTGGAGGCGC TCCGGGAAGA GGCTGAAAAG 
CAGCGAGCGG CTGCAGATGG TCTCCGGAGG GAGCTGGCCT CCCAGGCTGA GCATGCAGAG 
GGCCTGGGAC AGGAACTGAA GACTTGGCGT GAGAGGCTAT CCCAGAAGGA GCAGTCCCTG 
TCCTCACTGC AGCAAGAGCT TGCCACCTCC AAGGCCCTGG TTGGGGAACT GTTGCCCATC 
AAGCACCTCT GCCAGCAGTT AGAGGCCGAG AAGGGCACCT TGCAGAGCCG GCACCGAGAG 
GAGCTTGAGC AGGGTGAGAA GGCTGTGGCG GCGCTGCAGG CAGAGCTTCT TCGGGCCCGC 
CGAGAGCTTG GAGACCTCAC TGCATTGCGA CAGAAAGTGA CTGAGCAGGA GCAGATGGCT 
CAGCAGCTGA GGGCAGAGAA TGCCAGCTAT GCTGACCAAC TGAGTGCACT ACAACAGGCC 
CATAGCAGGC TAGCAGAGGA GAACCGAAAC CTGGGGGAGC GGGCCAGCCA CGGGCGGCAG 
CAGCTGGAGG CTGAGCTTGG ACGAGCCCAG GAACTTGAGG CAGTGCGGGC TGAGGCAGAA 
GCCCAGGCAG TTCGGAGCCG GGAGGAGGCC CTAGATACAG CCAGGCAGCT GGAGACCATG 
ACGGCCAAGT ATGAGAGTGC CAAGAGCAAG GTCTTGGAGG AGAGGCAGAG GTTCCAGGAA 
GAGCGGCAGA AACTCACTGC CCAGGTGGAG CAACTAGAGG CATTTCAGAG AGAACAGACT 
AAACAGGTGG AGGAACTGAA TAAGAAGCTG GCCCAGCAAC AAAATATGAA GCCCCCTGAA 
AGTGAAATAC AGCAGCAAGC TCATCATTAC CAGGCCCAAC TCAGTGAGCT GCAGGCCCAA 
CTGAGCCAGA AGGAACAGGC TGCTGAGCAC TATAAGGTGC AGATGGAAAG AGCCAAGGCT 
CACTATGATG CCAAGAAGCA GCAGAACCAG GAGCTGCAGG AACGTCTGCA GAGCTTGGAG 
AATCTACAGA AGGAGAATGC TGAACTGCGG ACCGAGTCTG AGAAGCTCGG CTGGGAACTG 
AAGCAAGCTG GGCTGAAGAC CAAGGAAGCT GAGCAAGCCT GCCGCCATCT TACTGCCCAG 
GTTCGAACTC TGGAGGCACA GGTTGCTCAT GCAGATCAGC AGCTCCGAGA TCTTGGCAAG 
TTTCAAGTGG CAACAGATGC ACTGAAAACC CGAGAAGTCC GAGGGCTGCC CCAACTGGAT 
CTGAGCACTG ACAGCCTAGA ATTGAGCAGT GAGGAAGGGA CCCCACTGAC CTCTACAAGA 
TCAGCAGCTC ACCGTACTCG AAAGTCATCT CGAACCCAGC CTGATGGCAC CAGCCTCCCT 
GGGGAGCCAG CTTCACCCAT CTCTCAGCGC CTGCCTTCTA AGGTGGAGTC CCTGGAGAGC 
TTGTACTTCA CCCCAATACC TGCCCGAGGA CAGGCCCCCT TAGAAAGCAG CATGGACTCA 
CTGGGTGACA TTGTATTGGA TTCTGGTCCC AAGACCCGCT CAGCCCGAAG GCGGACCACA 
CAGATCATTG ACATCACAAT GCTGTCCAGG AAACTGGACA CAGATGAACC GGACAGTTCG 
AATTCATCCT TCTACAGCAC ACAGTCAGCC CCTGCCCCAC AGCGGGGTCT CCGTTCTGCG 
TCTTCGGCTC AGTCCCTGGC TAACTGTCCC TCTCGGGAGT CCCGGACCAG GCTGGGCTCC 
CTGGAAGATG GGAACTCAGC CTTGCTCAGC CTTCCTGGTT ATAGGCCCAA CACTCGAAGC 
TCAGCCCGGC ACTCCCAGGC CAGAGCATCT AGCGGGGCCC CTTCAGGTCG GAGCAGCTTC 
TATACAGGCA CCTGCCAGGA TGAGCCAGAG CAGCTGGATG ACTGGAACCG GATTGCAGAA 
CTTCAGCAGC GCAACCGTGT CTGCCTCCCA CACCTGAAGA CCTCTTACCC CTTGGAGGCC 
AGGCCTTCCA TGAGCCTGGC CACCATCACA GACGAAGAGA TGAAGACGGG AGACCCCCAG 
GAGACCCTTC GCCGAGCCAG CATGCAGCCA GCCCAGATCC ATGAGTTTAG TGGCACACAT 
CGCCTTCCCT CAAGCATCAC CACCCGGCAG CACCGGAAGC GCATCTCCTC GGAGCTTGAC 
CAAGGCCTCG GCACCCCTGA GGCCAAGAAA CCGACCAGCT GTTTTCCTCG TCCCCTCACC 
CCCCGGGACA GACATGAAGG CCGGAAGATG AGTGCCGCCG AGGGCCAGAA GAAGGGGGCC 
CCTGCCCCCG GCAAGCAGAC CAACAAGCGC CAGTCATTAG CCTTCAACAT TCTGAACACA 
CCCAAGAGAC TGGGGAACAG CCTGCTCCGG GGAGCCACCA GGAAATCTTC GGCTAAGACC 
TCCCCACTCC CCCGAAGTGG GACCCGCCGC TCTCCCCGAA TTGCCACTAC TACCATCACC 
ACTGGCAGCA CCTCTCCCCG GGCCAGGGGC AAGGCAAAGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NUMA1 ( XM_007491052.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007491114.1 Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X7, mRNA.

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CloneID	OMc22045
Clone ID Related Accession (Same CDS sequence)	XM_007491050.1 , XM_007491051.2 , XM_007491053.2 , XM_007491052.1
Accession Version	XM_007491052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	nuclear mitotic apparatus protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008804.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCTTC ATGCGACCAG GACAGCTGCA CTCCTCTCTT GGGTGAACAG CCTACAGATT 
GCTGAACCTG TGACATCAGT GCTAGAGCTC CAAGACTGCA ACATCTTTAT CCAGATCATC 
AACCGCATCC ATGGCACTGA AGAGGGGCAG CAGATCCTGG AGCAGCCTCT GCTGGACAGA 
CTCAACTTCA TCGCCAACTT TCTGCAGAAA AACTGCAAAC ATCGGTCCTC CTCAGAGAGC 
CTAGTGTCTG TGCAGAAGCT AGAAGATGGG GAGGAGTTGG AGTTGGCCAA GGTGATCATG 
TTACTCTTAT ACCATTCCAC CATGAGTTCC AAAAGTTTGA GGGATTGGAA CCAATTTGAC 
TACAAAATCC AGTCGGAGTT GGCTGCCATC CTTAAATTTG TGTTGGACCA TGAAGATGGG 
TTCAGTCTGA ATGACAACTT AGAGAGTTTC CTTCAGAAAG CTCCTCTCCT TTCCAGTAGT 
CCTAGCACCA GCTCTGATGA GCTTTCCCCA ATGCGTACCC AACAAACCAG GAAGGAAGTT 
CGATTCTTGG AACTGCAGAA GATTGCCTCC TCATCAGGTC TAAACAACTT CTTGTCAGGG 
TCTCCTGCTT CTCCCATGGG TGATATCCTT CAGACCCCCC AGTTCCAAAT GAGACGGTTG 
AAGAAACAGC TGGCAGATGA GCGGGCTAGC CGAGATGAGT TGGAGCTGGA GTTAGCCAAA 
AGCTGCCAAC TACTGGCTGA GAAGGACACA CAGATTTCCA CGATGCAGCA GCGTATTGAT 
CGCTTGGCTC TCCTAAATGA GAAGCAGGCC ACAGGACCCC TGGAATCTAA AGAGCTGGAA 
GAATTACGTG ACAAGAATGA GAGTCTGATT GTACGGTTTC ATGAAGCCTT GAAACAGTGC 
CAGGACTTGA AGACTGAGAA GGGCCAGATG GAGCGCAAGA TTGACCAGCT CTCTGAGGAG 
AATGGGGACC TTTCTTTCAA GCTTCGGGAT TTTGCAAGCC ATCTGCTGCA GCTACAGGAG 
GCTCTCAATG ATCTGACAGA AGAACATAAT AGCTCCACAA AGGAATGGAG GGAGAAACAG 
GTTCACCTGG AGACAGAACT TGGCACCACC CTGCAAGACA AGAAATGCCT TGAAGAGAAG 
AATGAGATCC TCCAGGGAAA AATATCACAG TTGGAGGAGC AGCTGGCACA GGTTGGGAAG 
AATCCATCTC AAGAGAAGGG AGAGGTGTTG GGCGACATCT TGCAGCTGGA AGCCCTGAAA 
CAAGAAGTAG CCAACTTTGC TGCAAAGGAT GCTGAGCTTC AAGCCACAAT AAAGCAACTG 
GAGGATGAAC GGAATCAGTG GAAATTAGAG ATGCAAGCTG AGCAGGACCG CTTTAAGGAA 
GAGAAGCAGC AGCTGGAGCA GAAGGCTCAG ACTCAGGAAG CCCAGCTGAC TGCTCAGGTG 
GCCACCCTCC ATGCCACCCT CCAGCAGCAG GACCATGATC TGGCTGACCT CAAGCAGCAG 
ACTGAAAAGG AACAAGCACA ACTGACTGAG ACCCTTCAAC AACAAGAGCT GGCTTCCCAA 
GGCCTCCAGC GCCAGGTGGA GCAACTGAGC AACAAGCTAA AGCAAGAGGA GGGAAGGCTG 
GAGGGACTGG TCCAGGATTG GAAGGTGACC AGCCAGACCA AGGACCAACA GCTGGCAGCA 
GCTATGGAGA AGTACCAGGA GGCCCAGCAA GAGAGGGATG CAGCCCTGAA ACAGTTGAAG 
GAGTTGGAGA AAACGAAAGC TGTGGAGTTG GGAGCTCTGC AACAGCAGCT CCAAGCTACC 
AAGGATGCCT GTAACAGTGC CCAGTCTTGT GCTGCAAAGG CTGAGCAGGA GAAGACCATA 
CTGAGCCAGA AGGTGGAGGA GCTTAGCACG TGTGTAGTTG AACTGAGGAC CTGGCAAGAG 
GAGCACAAAG CAGAAGCTAA ATTGGCAGAA AGCCTTCAGA CTCGGGTCGA GGAACTGGAG 
AACCAGCTGA AGGCTGAACA ACAGAAACTA GCAGAGAGAG AAGAACTAGC TCGGGAGAAT 
GCCCAGCTCC AGGAACGACT TCTTTCCCTG GAAGATTCGT TGCAGAATAC TAAGGGCATC 
CTGGAAGATG AGAAGCGACA AGCTGTAGAG GCCCTGGAGG GGCAACTACG CCGCATTTCT 
GAGCTGGAGG CAGAGACGCA GAGCCTTGGG CAGCAGCGAG ACCAAGATCG GCGGCAGCTG 
GAGGAGGCAA AGGCTGGGCA TCAGGGTCTG GAGGCCCAGC TCCAGAAGCT TGGAGAAGAG 
CATCGTTCCA AAGTAGAAAC CTTGCAGCAG GAACTGGAAA AGGTGGCAGC TGCCCGGCAA 
GAGACAGAGG GAAAGTGCAA GCAGCTGGCC TCAGAGGCAG CTTCCTGGCG TACTCGTTAT 
GAAGAGAACC AGAAGGAGGG GGCGCAGCGT GGTGCCCTGC ATGAGGAACA GTTGAAGGCC 
CTGAATGAGG AGCAAGAGAA AACCCAAAAG CAGCTGCAGG CTGCCCAGGA GAGATTGGCA 
GTGCTGGAGG CTCTCACCAA ACGCCAGGAT GCTAAACAGC AAGAATACAC TCGGCTTGAG 
GCTGACCTGT CAGGTGCCCT CCGGCAGCTC CAGGAGAAGG AAGAGCGGGC CCAGGATCTG 
GCCGACAGCC TGTGCAAGCT GCGGGAGGAG GTAGATCAGC TCACAGCTGC AGTCAGGAAA 
GCAGATGAGG AGAAGGAAGC CTCTTCTCAG AGGCTGGCTG AAGAGCAGCA ACGGGTGCAA 
GAGCTTGAGG CTGCCACAAA GCAGCTGGAA GAGCAGCAGA ACCAGAAACT AAGCAGGATC 
CAGGCGGCCC TGCAGGCTAA GGAGCAAGAA GCAGAGCAGC TGACAGGAAA GCTAGAGCAA 
GCACGCGGTG CTCTGGCAGA GAGCCGGGGG CACCAGGAGG AAGAGCGTGT CCAGTGGGAG 
CGGGAAAGAG TCCGTGCAGA GGTGGAACGA GACCAGGCCA GAGCCGACCT GGCCACTGAG 
AAGGCTGCTA AGGCAGAACT GGAGGCAAGG CTGCAGAGCT CCCTGAGTGA GCAGCGGGTG 
GAGTTCAGTG CCCTGCAGGA AGCCTTGGCC CATGCCCTGG AAGGAGCAGA AGGGAAGGAA 
CAGCAGCTTG GAGAGCTTCA GAAGCAAGAG GCCGCCCTGC GGGCAGAACT GACTAAGCTG 
CAGAAGACCA CTGAGGACTT GAGAGAAAGG TTGGCTCAGG AGGAGGCAGC TGCCCAAGCA 
GATGCTCTGG GGCCTGCACA GTCGGGCGCC CAGGAGTCAG AGGCTCTGCG GGCCGAGCTG 
GGTGAGCTGG AGCAGCGGTG CCGGGAGCAG GAGGAGAAGG CCTCTGAGCT GGAGCGCCGC 
CTTGAGGCTT CTCGGAAGGC CCAGGCCAGT CAGGAAAACA CTACTGCTGC CCTCCGGGCT 
CAGCTGGAGG AAAGCACCCA AGAGCTAGCT GCCCTCCGAG TGGCAGCTCA GGAGCACAGC 
AAGGCTGAAG AGGAGCAAAA AGCCCAGGTG GCCCATGCCC AGCAGGAGGC AGAGAAGAAT 
AGCAGCCTCA TCAGCAGCTT GGAGCAGGAG GTGTCCATCT TGAACCTACA GGTTCTGGAG 
AAAGAAGGAG AAAGCAAAGA GCTGAAGCGT CTAGTGCTGG CTGAGTCAGA GAAAAGCCAG 
AAGCTGGAGG AGCGCCTGCG CCTGCTTCAG ACAGAAACTG CCAGTGCCAG CACACGGGCG 
GCAGAGTGCA GCGCAGCCCT GAGCTCTGAG GTGGAGGCGC TCCGGGAAGA GGCTGAAAAG 
CAGCGAGCGG CTGCAGATGG TCTCCGGAGG GAGCTGGCCT CCCAGGCTGA GCATGCAGAG 
GGCCTGGGAC AGGAACTGAA GACTTGGCGT GAGAGGCTAT CCCAGAAGGA GCAGTCCCTG 
TCCTCACTGC AGCAAGAGCT TGCCACCTCC AAGGCCCTGG TTGGGGAACT GTTGCCCATC 
AAGCACCTCT GCCAGCAGTT AGAGGCCGAG AAGGGCACCT TGCAGAGCCG GCACCGAGAG 
GAGCTTGAGC AGGGTGAGAA GGCTGTGGCG GCGCTGCAGG CAGAGCTTCT TCGGGCCCGC 
CGAGAGCTTG GAGACCTCAC TGCATTGCGA CAGAAAGTGA CTGAGCAGGA GCAGATGGCT 
CAGCAGCTGA GGGCAGAGAA TGCCAGCTAT GCTGACCAAC TGAGTGCACT ACAACAGGCC 
CATAGCAGGC TAGCAGAGGA GAACCGAAAC CTGGGGGAGC GGGCCAGCCA CGGGCGGCAG 
CAGCTGGAGG CTGAGCTTGG ACGAGCCCAG GAACTTGAGG CAGTGCGGGC TGAGGCAGAA 
GCCCAGGCAG TTCGGAGCCG GGAGGAGGCC CTAGATACAG CCAGGCAGCT GGAGACCATG 
ACGGCCAAGT ATGAGAGTGC CAAGAGCAAG GTCTTGGAGG AGAGGCAGAG GTTCCAGGAA 
GAGCGGCAGA AACTCACTGC CCAGGTGGAG CAACTAGAGG CATTTCAGAG AGAACAGACT 
AAACAGGTGG AGGAACTGAA TAAGAAGCTG GCCCAGCAAC AAAATATGAA GCCCCCTGAA 
AGTGAAATAC AGCAGCAAGC TCATCATTAC CAGGCCCAAC TCAGTGAGCT GCAGGCCCAA 
CTGAGCCAGA AGGAACAGGC TGCTGAGCAC TATAAGGTGC AGATGGAAAG AGCCAAGGCT 
CACTATGATG CCAAGAAGCA GCAGAACCAG GAGCTGCAGG AACGTCTGCA GAGCTTGGAG 
AATCTACAGA AGGAGAATGC TGAACTGCGG ACCGAGTCTG AGAAGCTCGG CTGGGAACTG 
AAGCAAGCTG GGCTGAAGAC CAAGGAAGCT GAGCAAGCCT GCCGCCATCT TACTGCCCAG 
GTTCGAACTC TGGAGGCACA GGTTGCTCAT GCAGATCAGC AGCTCCGAGA TCTTGGCAAG 
TTTCAAGTGG CAACAGATGC ACTGAAAACC CGAGAAGTCC GAGGGCTGCC CCAACTGGAT 
CTGAGCACTG ACAGCCTAGA ATTGAGCAGT GAGGAAGGGA CCCCACTGAC CTCTACAAGA 
TCAGCAGCTC ACCGTACTCG AAAGTCATCT CGAACCCAGC CTGATGGCAC CAGCCTCCCT 
GGGGAGCCAG CTTCACCCAT CTCTCAGCGC CTGCCTTCTA AGGTGGAGTC CCTGGAGAGC 
TTGTACTTCA CCCCAATACC TGCCCGAGGA CAGGCCCCCT TAGAAAGCAG CATGGACTCA 
CTGGGTGACA TTGTATTGGA TTCTGGTCCC AAGACCCGCT CAGCCCGAAG GCGGACCACA 
CAGATCATTG ACATCACAAT GCTGTCCAGG AAACTGGACA CAGATGAACC GGACAGTTCG 
AATTCATCCT TCTACAGCAC ACAGTCAGCC CCTGCCCCAC AGCGGGGTCT CCGTTCTGCG 
TCTTCGGCTC AGTCCCTGGC TAACTGTCCC TCTCGGGAGT CCCGGACCAG GCTGGGCTCC 
CTGGAAGATG GGAACTCAGC CTTGCTCAGC CTTCCTGGTT ATAGGCCCAA CACTCGAAGC 
TCAGCCCGGC ACTCCCAGGC CAGAGCATCT AGCGGGGCCC CTTCAGGTCG GAGCAGCTTC 
TATACAGGCA CCTGCCAGGA TGAGCCAGAG CAGCTGGATG ACTGGAACCG GATTGCAGAA 
CTTCAGCAGC GCAACCGTGT CTGCCTCCCA CACCTGAAGA CCTCTTACCC CTTGGAGGCC 
AGGCCTTCCA TGAGCCTGGC CACCATCACA GACGAAGAGA TGAAGACGGG AGACCCCCAG 
GAGACCCTTC GCCGAGCCAG CATGCAGCCA GCCCAGATCC ATGAGTTTAG TGGCACACAT 
CGCCTTCCCT CAAGCATCAC CACCCGGCAG CACCGGAAGC GCATCTCCTC GGAGCTTGAC 
CAAGGCCTCG GCACCCCTGA GGCCAAGAAA CCGACCAGCT GTTTTCCTCG TCCCCTCACC 
CCCCGGGACA GACATGAAGG CCGGAAGATG AGTGCCGCCG AGGGCCAGAA GAAGGGGGCC 
CCTGCCCCCG GCAAGCAGAC CAACAAGCGC CAGTCATTAG CCTTCAACAT TCTGAACACA 
CCCAAGAGAC TGGGGAACAG CCTGCTCCGG GGAGCCACCA GGAAATCTTC GGCTAAGACC 
TCCCCACTCC CCCGAAGTGG GACCCGCCGC TCTCCCCGAA TTGCCACTAC TACCATCACC 
ACTGGCAGCA CCTCTCCCCG GGCCAGGGGC AAGGCAAAGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007491114.1
CDS	111..6335
Translation	MTLHATRTAALLSWVNSLQIAEPVTSVLELQDCNIFIQIINRIHGTEEGQQILEQPLLDRLNFIANFLQKNCKHRSSSESLVSVQKLEDGEELELAKVIMLLLYHSTMSSKSLRDWNQFDYKIQSELAAILKFVLDHEDGFSLNDNLESFLQKAPLLSSSPSTSSDELSPMRTQQTRKEVRFLELQKIASSSGLNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERASRDELELELAKSCQLLAEKDTQISTMQQRIDRLALLNEKQATGPLESKELEELRDKNESLIVRFHEALKQCQDLKTEKGQMERKIDQLSEENGDLSFKLRDFASHLLQLQEALNDLTEEHNSSTKEWREKQVHLETELGTTLQDKKCLEEKNEILQGKISQLEEQLAQVGKNPSQEKGEVLGDILQLEALKQEVANFAAKDAELQATIKQLEDERNQWKLEMQAEQDRFKEEKQQLEQKAQTQEAQLTAQVATLHATLQQQDHDLADLKQQTEKEQAQLTETLQQQELASQGLQRQVEQLSNKLKQEEGRLEGLVQDWKVTSQTKDQQLAAAMEKYQEAQQERDAALKQLKELEKTKAVELGALQQQLQATKDACNSAQSCAAKAEQEKTILSQKVEELSTCVVELRTWQEEHKAEAKLAESLQTRVEELENQLKAEQQKLAEREELARENAQLQERLLSLEDSLQNTKGILEDEKRQAVEALEGQLRRISELEAETQSLGQQRDQDRRQLEEAKAGHQGLEAQLQKLGEEHRSKVETLQQELEKVAAARQETEGKCKQLASEAASWRTRYEENQKEGAQRGALHEEQLKALNEEQEKTQKQLQAAQERLAVLEALTKRQDAKQQEYTRLEADLSGALRQLQEKEERAQDLADSLCKLREEVDQLTAAVRKADEEKEASSQRLAEEQQRVQELEAATKQLEEQQNQKLSRIQAALQAKEQEAEQLTGKLEQARGALAESRGHQEEERVQWERERVRAEVERDQARADLATEKAAKAELEARLQSSLSEQRVEFSALQEALAHALEGAEGKEQQLGELQKQEAALRAELTKLQKTTEDLRERLAQEEAAAQADALGPAQSGAQESEALRAELGELEQRCREQEEKASELERRLEASRKAQASQENTTAALRAQLEESTQELAALRVAAQEHSKAEEEQKAQVAHAQQEAEKNSSLISSLEQEVSILNLQVLEKEGESKELKRLVLAESEKSQKLEERLRLLQTETASASTRAAECSAALSSEVEALREEAEKQRAAADGLRRELASQAEHAEGLGQELKTWRERLSQKEQSLSSLQQELATSKALVGELLPIKHLCQQLEAEKGTLQSRHREELEQGEKAVAALQAELLRARRELGDLTALRQKVTEQEQMAQQLRAENASYADQLSALQQAHSRLAEENRNLGERASHGRQQLEAELGRAQELEAVRAEAEAQAVRSREEALDTARQLETMTAKYESAKSKVLEERQRFQEERQKLTAQVEQLEAFQREQTKQVEELNKKLAQQQNMKPPESEIQQQAHHYQAQLSELQAQLSQKEQAAEHYKVQMERAKAHYDAKKQQNQELQERLQSLENLQKENAELRTESEKLGWELKQAGLKTKEAEQACRHLTAQVRTLEAQVAHADQQLRDLGKFQVATDALKTREVRGLPQLDLSTDSLELSSEEGTPLTSTRSAAHRTRKSSRTQPDGTSLPGEPASPISQRLPSKVESLESLYFTPIPARGQAPLESSMDSLGDIVLDSGPKTRSARRRTTQIIDITMLSRKLDTDEPDSSNSSFYSTQSAPAPQRGLRSASSAQSLANCPSRESRTRLGSLEDGNSALLSLPGYRPNTRSSARHSQARASSGAPSGRSSFYTGTCQDEPEQLDDWNRIAELQQRNRVCLPHLKTSYPLEARPSMSLATITDEEMKTGDPQETLRRASMQPAQIHEFSGTHRLPSSITTRQHRKRISSELDQGLGTPEAKKPTSCFPRPLTPRDRHEGRKMSAAEGQKKGAPAPGKQTNKRQSLAFNILNTPKRLGNSLLRGATRKSSAKTSPLPRSGTRRSPRIATTTITTGSTSPRARGKAKH

Target ORF information:

RefSeq Version	XM_007491052.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica nuclear mitotic apparatus protein 1 (NUMA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007491052.1

ORF Insert Sequence:

ATGACTCTTC ATGCGACCAG GACAGCTGCA CTCCTCTCTT GGGTGAACAG CCTACAGATT 
GCTGAACCTG TGACATCAGT GCTAGAGCTC CAAGACTGCA ACATCTTTAT CCAGATCATC 
AACCGCATCC ATGGCACTGA AGAGGGGCAG CAGATCCTGG AGCAGCCTCT GCTGGACAGA 
CTCAACTTCA TCGCCAACTT TCTGCAGAAA AACTGCAAAC ATCGGTCCTC CTCAGAGAGC 
CTAGTGTCTG TGCAGAAGCT AGAAGATGGG GAGGAGTTGG AGTTGGCCAA GGTGATCATG 
TTACTCTTAT ACCATTCCAC CATGAGTTCC AAAAGTTTGA GGGATTGGAA CCAATTTGAC 
TACAAAATCC AGTCGGAGTT GGCTGCCATC CTTAAATTTG TGTTGGACCA TGAAGATGGG 
TTCAGTCTGA ATGACAACTT AGAGAGTTTC CTTCAGAAAG CTCCTCTCCT TTCCAGTAGT 
CCTAGCACCA GCTCTGATGA GCTTTCCCCA ATGCGTACCC AACAAACCAG GAAGGAAGTT 
CGATTCTTGG AACTGCAGAA GATTGCCTCC TCATCAGGTC TAAACAACTT CTTGTCAGGG 
TCTCCTGCTT CTCCCATGGG TGATATCCTT CAGACCCCCC AGTTCCAAAT GAGACGGTTG 
AAGAAACAGC TGGCAGATGA GCGGGCTAGC CGAGATGAGT TGGAGCTGGA GTTAGCCAAA 
AGCTGCCAAC TACTGGCTGA GAAGGACACA CAGATTTCCA CGATGCAGCA GCGTATTGAT 
CGCTTGGCTC TCCTAAATGA GAAGCAGGCC ACAGGACCCC TGGAATCTAA AGAGCTGGAA 
GAATTACGTG ACAAGAATGA GAGTCTGATT GTACGGTTTC ATGAAGCCTT GAAACAGTGC 
CAGGACTTGA AGACTGAGAA GGGCCAGATG GAGCGCAAGA TTGACCAGCT CTCTGAGGAG 
AATGGGGACC TTTCTTTCAA GCTTCGGGAT TTTGCAAGCC ATCTGCTGCA GCTACAGGAG 
GCTCTCAATG ATCTGACAGA AGAACATAAT AGCTCCACAA AGGAATGGAG GGAGAAACAG 
GTTCACCTGG AGACAGAACT TGGCACCACC CTGCAAGACA AGAAATGCCT TGAAGAGAAG 
AATGAGATCC TCCAGGGAAA AATATCACAG TTGGAGGAGC AGCTGGCACA GGTTGGGAAG 
AATCCATCTC AAGAGAAGGG AGAGGTGTTG GGCGACATCT TGCAGCTGGA AGCCCTGAAA 
CAAGAAGTAG CCAACTTTGC TGCAAAGGAT GCTGAGCTTC AAGCCACAAT AAAGCAACTG 
GAGGATGAAC GGAATCAGTG GAAATTAGAG ATGCAAGCTG AGCAGGACCG CTTTAAGGAA 
GAGAAGCAGC AGCTGGAGCA GAAGGCTCAG ACTCAGGAAG CCCAGCTGAC TGCTCAGGTG 
GCCACCCTCC ATGCCACCCT CCAGCAGCAG GACCATGATC TGGCTGACCT CAAGCAGCAG 
ACTGAAAAGG AACAAGCACA ACTGACTGAG ACCCTTCAAC AACAAGAGCT GGCTTCCCAA 
GGCCTCCAGC GCCAGGTGGA GCAACTGAGC AACAAGCTAA AGCAAGAGGA GGGAAGGCTG 
GAGGGACTGG TCCAGGATTG GAAGGTGACC AGCCAGACCA AGGACCAACA GCTGGCAGCA 
GCTATGGAGA AGTACCAGGA GGCCCAGCAA GAGAGGGATG CAGCCCTGAA ACAGTTGAAG 
GAGTTGGAGA AAACGAAAGC TGTGGAGTTG GGAGCTCTGC AACAGCAGCT CCAAGCTACC 
AAGGATGCCT GTAACAGTGC CCAGTCTTGT GCTGCAAAGG CTGAGCAGGA GAAGACCATA 
CTGAGCCAGA AGGTGGAGGA GCTTAGCACG TGTGTAGTTG AACTGAGGAC CTGGCAAGAG 
GAGCACAAAG CAGAAGCTAA ATTGGCAGAA AGCCTTCAGA CTCGGGTCGA GGAACTGGAG 
AACCAGCTGA AGGCTGAACA ACAGAAACTA GCAGAGAGAG AAGAACTAGC TCGGGAGAAT 
GCCCAGCTCC AGGAACGACT TCTTTCCCTG GAAGATTCGT TGCAGAATAC TAAGGGCATC 
CTGGAAGATG AGAAGCGACA AGCTGTAGAG GCCCTGGAGG GGCAACTACG CCGCATTTCT 
GAGCTGGAGG CAGAGACGCA GAGCCTTGGG CAGCAGCGAG ACCAAGATCG GCGGCAGCTG 
GAGGAGGCAA AGGCTGGGCA TCAGGGTCTG GAGGCCCAGC TCCAGAAGCT TGGAGAAGAG 
CATCGTTCCA AAGTAGAAAC CTTGCAGCAG GAACTGGAAA AGGTGGCAGC TGCCCGGCAA 
GAGACAGAGG GAAAGTGCAA GCAGCTGGCC TCAGAGGCAG CTTCCTGGCG TACTCGTTAT 
GAAGAGAACC AGAAGGAGGG GGCGCAGCGT GGTGCCCTGC ATGAGGAACA GTTGAAGGCC 
CTGAATGAGG AGCAAGAGAA AACCCAAAAG CAGCTGCAGG CTGCCCAGGA GAGATTGGCA 
GTGCTGGAGG CTCTCACCAA ACGCCAGGAT GCTAAACAGC AAGAATACAC TCGGCTTGAG 
GCTGACCTGT CAGGTGCCCT CCGGCAGCTC CAGGAGAAGG AAGAGCGGGC CCAGGATCTG 
GCCGACAGCC TGTGCAAGCT GCGGGAGGAG GTAGATCAGC TCACAGCTGC AGTCAGGAAA 
GCAGATGAGG AGAAGGAAGC CTCTTCTCAG AGGCTGGCTG AAGAGCAGCA ACGGGTGCAA 
GAGCTTGAGG CTGCCACAAA GCAGCTGGAA GAGCAGCAGA ACCAGAAACT AAGCAGGATC 
CAGGCGGCCC TGCAGGCTAA GGAGCAAGAA GCAGAGCAGC TGACAGGAAA GCTAGAGCAA 
GCACGCGGTG CTCTGGCAGA GAGCCGGGGG CACCAGGAGG AAGAGCGTGT CCAGTGGGAG 
CGGGAAAGAG TCCGTGCAGA GGTGGAACGA GACCAGGCCA GAGCCGACCT GGCCACTGAG 
AAGGCTGCTA AGGCAGAACT GGAGGCAAGG CTGCAGAGCT CCCTGAGTGA GCAGCGGGTG 
GAGTTCAGTG CCCTGCAGGA AGCCTTGGCC CATGCCCTGG AAGGAGCAGA AGGGAAGGAA 
CAGCAGCTTG GAGAGCTTCA GAAGCAAGAG GCCGCCCTGC GGGCAGAACT GACTAAGCTG 
CAGAAGACCA CTGAGGACTT GAGAGAAAGG TTGGCTCAGG AGGAGGCAGC TGCCCAAGCA 
GATGCTCTGG GGCCTGCACA GTCGGGCGCC CAGGAGTCAG AGGCTCTGCG GGCCGAGCTG 
GGTGAGCTGG AGCAGCGGTG CCGGGAGCAG GAGGAGAAGG CCTCTGAGCT GGAGCGCCGC 
CTTGAGGCTT CTCGGAAGGC CCAGGCCAGT CAGGAAAACA CTACTGCTGC CCTCCGGGCT 
CAGCTGGAGG AAAGCACCCA AGAGCTAGCT GCCCTCCGAG TGGCAGCTCA GGAGCACAGC 
AAGGCTGAAG AGGAGCAAAA AGCCCAGGTG GCCCATGCCC AGCAGGAGGC AGAGAAGAAT 
AGCAGCCTCA TCAGCAGCTT GGAGCAGGAG GTGTCCATCT TGAACCTACA GGTTCTGGAG 
AAAGAAGGAG AAAGCAAAGA GCTGAAGCGT CTAGTGCTGG CTGAGTCAGA GAAAAGCCAG 
AAGCTGGAGG AGCGCCTGCG CCTGCTTCAG ACAGAAACTG CCAGTGCCAG CACACGGGCG 
GCAGAGTGCA GCGCAGCCCT GAGCTCTGAG GTGGAGGCGC TCCGGGAAGA GGCTGAAAAG 
CAGCGAGCGG CTGCAGATGG TCTCCGGAGG GAGCTGGCCT CCCAGGCTGA GCATGCAGAG 
GGCCTGGGAC AGGAACTGAA GACTTGGCGT GAGAGGCTAT CCCAGAAGGA GCAGTCCCTG 
TCCTCACTGC AGCAAGAGCT TGCCACCTCC AAGGCCCTGG TTGGGGAACT GTTGCCCATC 
AAGCACCTCT GCCAGCAGTT AGAGGCCGAG AAGGGCACCT TGCAGAGCCG GCACCGAGAG 
GAGCTTGAGC AGGGTGAGAA GGCTGTGGCG GCGCTGCAGG CAGAGCTTCT TCGGGCCCGC 
CGAGAGCTTG GAGACCTCAC TGCATTGCGA CAGAAAGTGA CTGAGCAGGA GCAGATGGCT 
CAGCAGCTGA GGGCAGAGAA TGCCAGCTAT GCTGACCAAC TGAGTGCACT ACAACAGGCC 
CATAGCAGGC TAGCAGAGGA GAACCGAAAC CTGGGGGAGC GGGCCAGCCA CGGGCGGCAG 
CAGCTGGAGG CTGAGCTTGG ACGAGCCCAG GAACTTGAGG CAGTGCGGGC TGAGGCAGAA 
GCCCAGGCAG TTCGGAGCCG GGAGGAGGCC CTAGATACAG CCAGGCAGCT GGAGACCATG 
ACGGCCAAGT ATGAGAGTGC CAAGAGCAAG GTCTTGGAGG AGAGGCAGAG GTTCCAGGAA 
GAGCGGCAGA AACTCACTGC CCAGGTGGAG CAACTAGAGG CATTTCAGAG AGAACAGACT 
AAACAGGTGG AGGAACTGAA TAAGAAGCTG GCCCAGCAAC AAAATATGAA GCCCCCTGAA 
AGTGAAATAC AGCAGCAAGC TCATCATTAC CAGGCCCAAC TCAGTGAGCT GCAGGCCCAA 
CTGAGCCAGA AGGAACAGGC TGCTGAGCAC TATAAGGTGC AGATGGAAAG AGCCAAGGCT 
CACTATGATG CCAAGAAGCA GCAGAACCAG GAGCTGCAGG AACGTCTGCA GAGCTTGGAG 
AATCTACAGA AGGAGAATGC TGAACTGCGG ACCGAGTCTG AGAAGCTCGG CTGGGAACTG 
AAGCAAGCTG GGCTGAAGAC CAAGGAAGCT GAGCAAGCCT GCCGCCATCT TACTGCCCAG 
GTTCGAACTC TGGAGGCACA GGTTGCTCAT GCAGATCAGC AGCTCCGAGA TCTTGGCAAG 
TTTCAAGTGG CAACAGATGC ACTGAAAACC CGAGAAGTCC GAGGGCTGCC CCAACTGGAT 
CTGAGCACTG ACAGCCTAGA ATTGAGCAGT GAGGAAGGGA CCCCACTGAC CTCTACAAGA 
TCAGCAGCTC ACCGTACTCG AAAGTCATCT CGAACCCAGC CTGATGGCAC CAGCCTCCCT 
GGGGAGCCAG CTTCACCCAT CTCTCAGCGC CTGCCTTCTA AGGTGGAGTC CCTGGAGAGC 
TTGTACTTCA CCCCAATACC TGCCCGAGGA CAGGCCCCCT TAGAAAGCAG CATGGACTCA 
CTGGGTGACA TTGTATTGGA TTCTGGTCCC AAGACCCGCT CAGCCCGAAG GCGGACCACA 
CAGATCATTG ACATCACAAT GCTGTCCAGG AAACTGGACA CAGATGAACC GGACAGTTCG 
AATTCATCCT TCTACAGCAC ACAGTCAGCC CCTGCCCCAC AGCGGGGTCT CCGTTCTGCG 
TCTTCGGCTC AGTCCCTGGC TAACTGTCCC TCTCGGGAGT CCCGGACCAG GCTGGGCTCC 
CTGGAAGATG GGAACTCAGC CTTGCTCAGC CTTCCTGGTT ATAGGCCCAA CACTCGAAGC 
TCAGCCCGGC ACTCCCAGGC CAGAGCATCT AGCGGGGCCC CTTCAGGTCG GAGCAGCTTC 
TATACAGGCA CCTGCCAGGA TGAGCCAGAG CAGCTGGATG ACTGGAACCG GATTGCAGAA 
CTTCAGCAGC GCAACCGTGT CTGCCTCCCA CACCTGAAGA CCTCTTACCC CTTGGAGGCC 
AGGCCTTCCA TGAGCCTGGC CACCATCACA GACGAAGAGA TGAAGACGGG AGACCCCCAG 
GAGACCCTTC GCCGAGCCAG CATGCAGCCA GCCCAGATCC ATGAGTTTAG TGGCACACAT 
CGCCTTCCCT CAAGCATCAC CACCCGGCAG CACCGGAAGC GCATCTCCTC GGAGCTTGAC 
CAAGGCCTCG GCACCCCTGA GGCCAAGAAA CCGACCAGCT GTTTTCCTCG TCCCCTCACC 
CCCCGGGACA GACATGAAGG CCGGAAGATG AGTGCCGCCG AGGGCCAGAA GAAGGGGGCC 
CCTGCCCCCG GCAAGCAGAC CAACAAGCGC CAGTCATTAG CCTTCAACAT TCTGAACACA 
CCCAAGAGAC TGGGGAACAG CCTGCTCCGG GGAGCCACCA GGAAATCTTC GGCTAAGACC 
TCCCCACTCC CCCGAAGTGG GACCCGCCGC TCTCCCCGAA TTGCCACTAC TACCATCACC 
ACTGGCAGCA CCTCTCCCCG GGCCAGGGGC AAGGCAAAGC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NUMA1 cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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