NIPBL cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene
Clones
gRNAs

Summary

Gene Symbol	NIPBL
Entrez Gene ID	100014459
Full Name	NIPBL, cohesin loading factor
Gene Type	protein-coding
Organism	Monodelphis domestica(gray short-tailed opossum)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007487441.1	XP_007487503.1	nipped-B-like protein isoform X1
XM_007487443.2	XP_007487505.1	nipped-B-like protein isoform X2
XM_007487444.2	XP_007487506.1	nipped-B-like protein isoform X3

Pathways from BioSystems
Gene Ontology

The following NIPBL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIPBL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMc15946	XM_007487444.2 Latest version!	Monodelphis domestica NIPBL, cohesin loading factor (NIPBL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OMc15944	XM_007487441.1 Latest version!	Monodelphis domestica NIPBL, cohesin loading factor (NIPBL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OMc15945	XM_007487443.2 Latest version!	Monodelphis domestica NIPBL, cohesin loading factor (NIPBL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NIPBL ( XM_007487444.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007487506.1 Monodelphis domestica NIPBL, cohesin loading factor (NIPBL), transcript variant X3, mRNA.

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CloneID	OMc15946
Clone ID Related Accession (Same CDS sequence)	XM_007487444.2
Accession Version	XM_007487444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	nipped-B-like protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007487444.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGATA AGCCTTTGAA AAAAAGAAAA CAAGATTCTT ACCCACAGGA GGCTGGGGGT 
GCTACTGGAG GTAATAGACC AGCTTCTCAG GAGACAGGTT CTACAGGAAA TAATGGGTCA 
AGGCCAGCTT TAATGGTTAG CATTGATCTT CATCAGGCAG GACGAGTAGA GTCTCAGGCA 
TCTGTAACTC AGGATTCAGA CACCATTAAA AAGCCTGAAG AAAGCAAACA GTGTAACGAT 
GGGGCTATTT CTGTTCATCA AGAAGATATT GGAGTTCTTA AACAAAAACT TGAAAACCAT 
CCTGAGACCC CTAGGAAAAA GTCTGATACT GAGAGTGTAA AGACTGACAT GAAACAAAAT 
GAGAACAAAT TGATGGAATC TAAACCAAAT GAAAACAGAT TGTTGGAGAC AAAACTGAAT 
GAGAGCAGAC CAGCAGCAGA AACTAAACAG AATGAGAGCA GACCAGCAGC AGAACCTAAA 
CAGATTGAGA GCAGATCAAC AGTAGAAACT AAGCAAACTG AGAGCAAATC AATGCCAGAA 
GTTAAACAGA CTGAGGGCAA ATCATTGGTG GACGTTAAGC ATAATGAGAG TAAACCAGCA 
GCAGAAGCTA AGTCCAATGA GAACAGAGCC ACAGTAGTGG AAGCAAAACA GAATGAAAAT 
AAATCAACAG AAGCAAAACA GACTGAAAGT AGACCAGTGA CAGAAGTTAA ACAGACTGAG 
AGCAAAGTAG CAGCAGAAGC TAAACAAAAT GAGAATAAAG CAACTGAAAC CAAGCAGAAT 
GAAAGCAGAA CGACAGAAAC TAAACAAAAT GAGAGCAAAA CTGAATCAAA ACAAAATGAG 
AACAAAACAA CAGACGCAAA ACCAAATGAA AATAAACAAA ATAATGGTAA GCAGGAAACA 
TCCAAGGTGA GACCTGAAAC TCCCAAGCAG AAGGGTGAGG GCAGACCTGA AACTCCCAAG 
CAGAAGGGAG AGGGCAGACC TGAAACTCCC AAGCAGAAGG GTGAGGGCCG CCCTGAAACT 
CCTAAACAGA AGGGTGATGG CCGCCCTGAA ACTCCCAAAC AGAAGGGTGA TGGCCGCCCT 
GAAACTCCAA GACACCGGCA TGATAGGAGA GATTCTGGAA AGTCATCTAC AGAAAAGAAA 
CCTGAAACAA GACATAAACA GGATGTTAAA TCTGATTCTC CTCGAGTGAA ATCTGAAAGA 
TCTGAAACTT CAAAGCAAAG ACCAGATGGA CGATCTGTTT CTGAGTCATT AAGGCGCGAC 
CATGATAATA AACAGAAATC AGAGGATAAG ACTGAATCAG AGAGACATCG AGGAGATCAG 
TCCAGAATTC GAAGACCAGA TACATTAAGA TCCTCCAATA GAAATGAGCA TGATTCTAAA 
CATGGCATTA AATCTGATGG TTCAAAAACA GATAAACTAG AAAGAAAACA CAGACATGAA 
TCAGGGGAGT CCAGGGAAAG ACTGTCTTCT GGGGAACAGA AATCAAGACC TGATAGTCCT 
CGTATTAAAG AGAGTAGAGG CGATACTAGC AAAACAAGAT CTGATAAATC TGGTTTTAAA 
TCACCAAATA GTAAAGATGA TCGGAGGACA GATGGTAATA AAAGCAAAGT AGACAGTAAT 
AAAACACACC CTGACAATAA AGCAGATTTT CCCAGTTATT TGTTGGGGGG CAGATCTGGT 
GCATTGAAAA ATTTTGTCAT TCCGAAAATC AAGAGAGATA AAGATGGCAA TGTTACTCAG 
GAAACAAGGA AAATGGAACT TAAGGGGGAT CAGAAAGATA AAATTGAAAA AATGGGACTA 
GTTGAAGATC TAAATAAAGG AGCTAAGCCT GTAGTTGTCC TGCAGAAGCT ATCTTTGGAT 
GATGTTCAGA AATTAATTAA GGATAGAGAG GACAGATCAA GAAGTTCTCT TAAAACTAGC 
AAGAATAAGC CATCCAAATC AAATAAAGGT AGTATAGATC AGTCAGTGTT AAAAGAATTA 
CCCCCTGAAC TCCTGGCAGA AATTGAATCT ACCATGCCAC TTTGTGAACG TGTGAAAATG 
AACAAGCGCA AACGTAGCAC AGTTAATGAA AAGCCAAAAT ATGCTGAAAT CAGTTCGGAT 
GAAGACAATG ACAGTGAAGA TGCTTTTGAA TCTTCTCGGA AACGACATAA GAAGGACAAA 
GATGATGATA AAGCTTGGGA ATATGAAGAG CGTGACAGAC GAAGCTCTGG GGATCATAGG 
AGAAGTGGCC ATTCTCATGA AGGCAGGAGG AGTTCCGGTG GTGGCCGTTA TCGAAACCGC 
AGCCCATCGG ATTCAGACAT GGAAGATTAT TCTCCTCCTC CCAGCCTCAG TGAGGTTGCT 
CGAAAGATGA AGAAAAGAGA AAAGCAAAAG AAACGGAAAG CTTATGAACC AAAACTAACA 
CCGGAAGAAA TGATGGATTC TTCTACTTTT AAGAGGTTTA CTGCTTCAAT AGAGAATATT 
TTAGAAAATC TCGAAGACAT GGATTTTACA GCATTTGGTG ATGATGAAGA AATTCCTCAG 
GAACTGCTAT TAGGAAAACA TCAGCTTAGT GAATTGGGCA GTGAATCTGC TAAAATTAAA 
GCAATGGGAA TAATGGATAA GCTCGCAACT GATAAAACAG TAAAAGTTCT AAATATTTTG 
GAGAAAAATA TTCAGGATGG ATCTAAGCTT TCTACATTGT TAAATCATAA TAATGACACT 
GAAGAAGAGG AACGTCTGTG GAGAGACCTA ATTATGGAGA GAGTTACAAA ATCAGCAGAT 
GCTTGTCTTA CAGCTATTAA CATTATGACA TCACCTAACA TGCCAAAAGC TGTATATATT 
GAGGATGTAA TAGAAAGAGT TATACAATAC ACTAAATTTC ATTTACAAAA TACTCTTTAT 
CCTCAGTATG ATCCTGTCTA CAGAGTGGAT CCTCATGGGG GAGGAGTATT GACCTCAAAG 
GCAAAACGTG CCAAGTGTTC CACTCATAAG CAGAGGGTAA TCGTAATGCT TTATAACAAA 
GTTTGTGATA TCGTTAGCAG CTTAGCAGAA CTTTTGGAAA TACAGCTCCT AACTGATACA 
ACAATTCTTC AGATATCATC AATGGGAATA ACACCATTTT TTGTAGAAAA TGTCAGTGAA 
CTCCAGTTAT GTGCCATCAA ATTAGTAACT GCAGTATTCT CAAGGGATGA AAAACATAGG 
CAGGTGATTT TGGAAGAAAT TTTCACTTCA CTTGCAAGAT TGCCAACCAG CAAGAGGAGT 
CTAAGGAACT TCAGGTTGAA TAGTAGTGAT ATGGATGGGG AGCCTATGTA TATTCAGATG 
GTGACTGCAC TAGTTTTGCA ACTTATTCAA TGTGTAGTAC ATCTACCATC AGCAGAGAAA 
GATTCTAATT CAGAAGAGGA ATCAAACAAA AAAGTGGATC AGGATGTCGT CATTACTAAT 
TCTTATGAGA CAGCTATGAG AACTGCTCAG AATTTCCTCT CTATTTTCCT AAAAAAATGT 
GGGAGTAAGC AAGGAGAAGA AGATTACAGA CCACTCTTTG AAAATTTTGT TCAAGATCTA 
CTTTCCACAG TCAATAAGCC AGAATGGCCA GCTGCTGAAT TATTACTTAG CTTGTTAGGG 
AGACTCTTGG TTCATCAGTT CAGCAATAAG TCAACAGAGA TGGCTTTAAG AGTGGCATCT 
CTTGATTATC TTGGAACAGT AGCTGCACGA TTGAGGAAAG ATGCTGTTAC AAGCAAAATG 
GATCAAGGAT CTATCGCCCG AATTTTAAAA CAGGTTACAG GAGGGGAAGA TGAAATTCAA 
CAGTTGCAAA AGGCATTGCT AGATTACCTG GATGAAAACA CTGAAACTGA TCCTTCATTG 
GTGTTTTCTC GTAAGTTCTA TATAGCTCAG TGGTTTCGAG ACACAACTTT GGAAACAGAA 
AAAGCTATGA AATCACAGAA AGATGAGGAT TCATCAGAAG GGACACACCA TGCAAAGGAG 
GTTGAAACAA CTGGTCAAAT CATGCATCGA GCAGAAAGTC GGAAAAAATT TCTTCGAAGC 
ATTATTAAAA CTACACCTTC ACAGTTCAGT ACATTAAAGA TGAATTCTGA TACTGTGGAC 
TATGAAGATG CTTGCTTGAT TGTAAGATAC TTGGCCTCTA TGAGGCCGTT TGCCCAGAGC 
TTCGATATTT ATTTGACACA GATTCTGCGA GTACTTGGTG AAAATGCAAT AGCTGTTCGA 
ACAAAAGCCA TGAAGTGTTT ATCAGAGGTT GTTGCTGTAG ATCCCAGCAT TTTAGCAAGG 
TTGGATATGC AACGAGGTGT CCATGGAAGG TTAATGGATA ATTCCACTAG TGTCCGTGAG 
GCAGCAGTTG AATTGTTAGG TCGATTTGTC CTCTGTCGAC CTCAGCTTGC TGAACAGTAT 
TACGACATGC TGATTGAACG AATATTGGAT ACTGGTATCA GTGTCAGGAA AAGGGTAATA 
AAGATTCTGA GAGATATATG TATTGAACAA CCTACATTTC CCAAAATCAC AGAGATGTGC 
GTGAAAATGA TCCGCAGAGT AAATGATGAA GAGGGCATTA AGAAATTAGT AAATGAAACA 
TTCCAGAAAC TCTGGTTTAC TCCAACTCCT CATCATGACA AGGAAGCAAT GACCAGAAAA 
ATTTTAAACA TCACTGATGT GGTTGCAGCT TGTAGAGATA CTGGATATGA CTGGTTTGAA 
CAGCTTCTTC AAAATCTTTT AAAATCAGAA GAAGATGCTT CTTATAAACC TGTGAAGAAA 
GCTTGTACTC AGCTTGTTGA TAACTTAGTT GAGCATATCC TTAAATATGA GGAATCTCTA 
GCTGATTCTG ACAATAAGGG AGTGAATTCT GGTCGATTGG TAGCTTGCAT AACCACTTTG 
TTCTTATTCA GCAAAATCAG ACCCCAGCTA ATGGTCAAAC ATGCCATGAC CATGCAACCA 
TACCTTACCA CTAAATGTAG CACACAAAAT GATTTCATGG TCATTTGCAA TGTTGCAAAA 
ATTTTAGAAT TAGTAGTACC ATTGATGGAG CACCCAAGTG AAACCTTCCT TGCCACCATT 
GAGGAAGATC TAATGAAACT TATCATCAAA TATGGCATGA CAGTAGTGCA ACATTGTGTG 
AGCTGTCTTG GGGCTGTTGT AAATAAAGTC ACACAAAATT ATAAATTTGT GTGGGCTTGT 
TTCAATAGAT ACTATGGTGC ACTTTCAAAA TTAAAAAGTC AGCACCAAGA AGACCCAAAC 
AGTACAATAC TTACAGCAAA TAAACCAGCA CTGCTTAGAT CTCTTTTCAC TGTCGGAGCA 
CTATGTCGGC ATTTTGATTT TGATCAAGAA GACTTTAAAG GCAGCAGTAA GGTTAACATA 
AAGGATAAAG TACTTGAACT CCTGATGTAT TTTACAAAAC ATTCAGATGA GGAAGTACAA 
ACCAAAGCCA TTATTGGTCT AGGATTTGCA TTTATTCAGC ATCCAAGTCT AATGTTTGAG 
CAAGAGGTAA AGAATTTATA TAACAGCATT CTAGCTGATA AAAACAGCTC AGTAAATTTA 
AAAATCCAAG TGTTAAAAAA TCTCCAGACC TACCTACAAG AAGAAGATAC ACGTATGCAA 
CAGGCAGATA AAGACTGGAA GAAAGTAGCA AAACAGGAAG ATCTGAAAGA AATGGGTGAT 
GTGTCCTCAG GGATGAGTAG CTCCATTATG CAGCTTTACC TCAAACAAGT TCTTGAGGCT 
TTCTTTCACA CTCAGTCAAG TGTGCGCCAC TTTGCCCTAA ATGTCATTGC ATTGACTCTC 
AACCAGGGGC TCATTCATCC TGTTCAGTGT GTGCCATATT TAATTGCTAT GGGCACAGAC 
CCTGAACCTG CTATGCGGAA TAAAGCCGAC CAGCAGCTTG TGGAAATAGA CAAAAAATAT 
GCTGGATTCA TTCACATGAA AGCTGTGGCT GGTATGAAGA TGTCTTACCA AGTTCAGCAA 
GCCATCAACA CATCCCCAAA AGATGCCGTG AGGGGCTTCA GACAAGATGA ATCCTCCAGT 
GCTTTATGTT CACATCTTTA CTCCATGATC CGTGGAAATC GCCAACACAG ACGAGCCTTT 
CTAATTGCTT TACTCAATCT CTTTGATGAC ACAGCAAAAA CAGAAGTGAA TATGCTCTTG 
TATATAGCAG ACAATCTAGC CTGTTTCCCT TACCAAACAC AGGAAGAACC ATTGTTTATA 
ATGCATCATA TAGATATTAC ACTCTCAGTT TCTGGCAGTA ACCTTCTGCA GTCATTTAAG 
GAGTCTATGG TGAAGGACAA GCGGAAAGAG AAGAAACCCT CACCAAATCG GGAAAGTGCA 
TCTGGAAGTG ATAGTGAAGG AGAGGTTTCC AAGCCTCGAA AGTCACGTAA ACGAGTTGAC 
TCTGATTCAG ATTCAGATGA TGAAGATGAC ATAAATGCTG TGATGAAATG TTTACCAGAT 
AATTCAAGTC CTTTAATTGA GTTTGCAAAT GTGTCCCAGG GTATTTTATT ACTTCTGATG 
TTAAAACAAC ATTTAAAGAA CCTATGTGGA TTCTCTGATA GTAAAATCCA GAAGTATTCT 
CCATCTGAAT CTGCAAAAGT ATATGATAAA GCGATAAACC GGAAAACAGG AATCCATTTT 
CATCCTAAAC AAACCTTAGA CTTCCTGCGG AGTGATATGG CTAATTCCAA AATTACAGAA 
GATGTGAAAA GGAGTATAGT TAAACAATAT CTAGATTTCA AGCTCCTCAT GGAACATCTG 
GATCCTGATG AAGAAGAAGA AGAAGGGGAG GTGTCAGCTA GCACAAATGC TCGGAACAAA 
GCAATTACCT CACTGCTTGG AGGAGGCAGC CCTAAAAATA ATGCAGCTGA GACAGAAGAT 
GATGAAAGTG ATGGGGAGGA TAGAGGAGGA GGCACTTCAG GGTCATTGAG AAGGTCAAAA 
CGAAATTCAG ACTCTACAGA GTTGGCAGCA CAGATGAATG AAAGTGTTGA TGTCATGGAT 
GTCATCGCTA TTTGCTGTCC AAAGTACAAA GATCGACCAC AAATTGCAAG AGTAGTACAG 
AAAACCAGCA ATGGCTTCAG TGTTCAGTGG ATGGCAGGCT CCTACAGTGG CTCCTGGACT 
GAGGCTAAGC GCCGTGATGG TCGCAAACTG GTGCCTTGGG TAGACACTAT TAAAGAGTCA 
GACATTATTT ACAAAAAAAT TGCTCTAACG AGTGCTAATA AGCTGACTAA TAAAGTTGTT 
CAGACTTTAC GATCGCTGTA TGCCGCCAAG GATGGGACTT CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007487506.1
CDS	140..7387
Translation	MRDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNNGSRPALMVSIDLHQAGRVESQASVTQDSDTIKKPEESKQCNDGAISVHQEDIGVLKQKLENHPETPRKKSDTESVKTDMKQNENKLMESKPNENRLLETKLNESRPAAETKQNESRPAAEPKQIESRSTVETKQTESKSMPEVKQTEGKSLVDVKHNESKPAAEAKSNENRATVVEAKQNENKSTEAKQTESRPVTEVKQTESKVAAEAKQNENKATETKQNESRTTETKQNESKTESKQNENKTTDAKPNENKQNNGKQETSKVRPETPKQKGEGRPETPKQKGEGRPETPKQKGEGRPETPKQKGDGRPETPKQKGDGRPETPRHRHDRRDSGKSSTEKKPETRHKQDVKSDSPRVKSERSETSKQRPDGRSVSESLRRDHDNKQKSEDKTESERHRGDQSRIRRPDTLRSSNRNEHDSKHGIKSDGSKTDKLERKHRHESGESRERLSSGEQKSRPDSPRIKESRGDTSKTRSDKSGFKSPNSKDDRRTDGNKSKVDSNKTHPDNKADFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETRKMELKGDQKDKIEKMGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDRSRSSLKTSKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSEDAFESSRKRHKKDKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKREKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILENLEDMDFTAFGDDEEIPQELLLGKHQLSELGSESAKIKAMGIMDKLATDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTAINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRVDPHGGGVLTSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLAELLEIQLLTDTTILQISSMGITPFFVENVSELQLCAIKLVTAVFSRDEKHRQVILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSAEKDSNSEEESNKKVDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIARILKQVTGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEDSSEGTHHAKEVETTGQIMHRAESRKKFLRSIIKTTPSQFSTLKMNSDTVDYEDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHHDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDASYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNYKFVWACFNRYYGALSKLKSQHQEDPNSTILTANKPALLRSLFTVGALCRHFDFDQEDFKGSSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNSILADKNSSVNLKIQVLKNLQTYLQEEDTRMQQADKDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTSPKDAVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLIALLNLFDDTAKTEVNMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKEKKPSPNRESASGSDSEGEVSKPRKSRKRVDSDSDSDDEDDINAVMKCLPDNSSPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGIHFHPKQTLDFLRSDMANSKITEDVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSNGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	XM_007487444.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica NIPBL, cohesin loading factor (NIPBL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007487444.2

ORF Insert Sequence:

ATGAGAGATA AGCCTTTGAA AAAAAGAAAA CAAGATTCTT ACCCACAGGA GGCTGGGGGT 
GCTACTGGAG GTAATAGACC AGCTTCTCAG GAGACAGGTT CTACAGGAAA TAATGGGTCA 
AGGCCAGCTT TAATGGTTAG CATTGATCTT CATCAGGCAG GACGAGTAGA GTCTCAGGCA 
TCTGTAACTC AGGATTCAGA CACCATTAAA AAGCCTGAAG AAAGCAAACA GTGTAACGAT 
GGGGCTATTT CTGTTCATCA AGAAGATATT GGAGTTCTTA AACAAAAACT TGAAAACCAT 
CCTGAGACCC CTAGGAAAAA GTCTGATACT GAGAGTGTAA AGACTGACAT GAAACAAAAT 
GAGAACAAAT TGATGGAATC TAAACCAAAT GAAAACAGAT TGTTGGAGAC AAAACTGAAT 
GAGAGCAGAC CAGCAGCAGA AACTAAACAG AATGAGAGCA GACCAGCAGC AGAACCTAAA 
CAGATTGAGA GCAGATCAAC AGTAGAAACT AAGCAAACTG AGAGCAAATC AATGCCAGAA 
GTTAAACAGA CTGAGGGCAA ATCATTGGTG GACGTTAAGC ATAATGAGAG TAAACCAGCA 
GCAGAAGCTA AGTCCAATGA GAACAGAGCC ACAGTAGTGG AAGCAAAACA GAATGAAAAT 
AAATCAACAG AAGCAAAACA GACTGAAAGT AGACCAGTGA CAGAAGTTAA ACAGACTGAG 
AGCAAAGTAG CAGCAGAAGC TAAACAAAAT GAGAATAAAG CAACTGAAAC CAAGCAGAAT 
GAAAGCAGAA CGACAGAAAC TAAACAAAAT GAGAGCAAAA CTGAATCAAA ACAAAATGAG 
AACAAAACAA CAGACGCAAA ACCAAATGAA AATAAACAAA ATAATGGTAA GCAGGAAACA 
TCCAAGGTGA GACCTGAAAC TCCCAAGCAG AAGGGTGAGG GCAGACCTGA AACTCCCAAG 
CAGAAGGGAG AGGGCAGACC TGAAACTCCC AAGCAGAAGG GTGAGGGCCG CCCTGAAACT 
CCTAAACAGA AGGGTGATGG CCGCCCTGAA ACTCCCAAAC AGAAGGGTGA TGGCCGCCCT 
GAAACTCCAA GACACCGGCA TGATAGGAGA GATTCTGGAA AGTCATCTAC AGAAAAGAAA 
CCTGAAACAA GACATAAACA GGATGTTAAA TCTGATTCTC CTCGAGTGAA ATCTGAAAGA 
TCTGAAACTT CAAAGCAAAG ACCAGATGGA CGATCTGTTT CTGAGTCATT AAGGCGCGAC 
CATGATAATA AACAGAAATC AGAGGATAAG ACTGAATCAG AGAGACATCG AGGAGATCAG 
TCCAGAATTC GAAGACCAGA TACATTAAGA TCCTCCAATA GAAATGAGCA TGATTCTAAA 
CATGGCATTA AATCTGATGG TTCAAAAACA GATAAACTAG AAAGAAAACA CAGACATGAA 
TCAGGGGAGT CCAGGGAAAG ACTGTCTTCT GGGGAACAGA AATCAAGACC TGATAGTCCT 
CGTATTAAAG AGAGTAGAGG CGATACTAGC AAAACAAGAT CTGATAAATC TGGTTTTAAA 
TCACCAAATA GTAAAGATGA TCGGAGGACA GATGGTAATA AAAGCAAAGT AGACAGTAAT 
AAAACACACC CTGACAATAA AGCAGATTTT CCCAGTTATT TGTTGGGGGG CAGATCTGGT 
GCATTGAAAA ATTTTGTCAT TCCGAAAATC AAGAGAGATA AAGATGGCAA TGTTACTCAG 
GAAACAAGGA AAATGGAACT TAAGGGGGAT CAGAAAGATA AAATTGAAAA AATGGGACTA 
GTTGAAGATC TAAATAAAGG AGCTAAGCCT GTAGTTGTCC TGCAGAAGCT ATCTTTGGAT 
GATGTTCAGA AATTAATTAA GGATAGAGAG GACAGATCAA GAAGTTCTCT TAAAACTAGC 
AAGAATAAGC CATCCAAATC AAATAAAGGT AGTATAGATC AGTCAGTGTT AAAAGAATTA 
CCCCCTGAAC TCCTGGCAGA AATTGAATCT ACCATGCCAC TTTGTGAACG TGTGAAAATG 
AACAAGCGCA AACGTAGCAC AGTTAATGAA AAGCCAAAAT ATGCTGAAAT CAGTTCGGAT 
GAAGACAATG ACAGTGAAGA TGCTTTTGAA TCTTCTCGGA AACGACATAA GAAGGACAAA 
GATGATGATA AAGCTTGGGA ATATGAAGAG CGTGACAGAC GAAGCTCTGG GGATCATAGG 
AGAAGTGGCC ATTCTCATGA AGGCAGGAGG AGTTCCGGTG GTGGCCGTTA TCGAAACCGC 
AGCCCATCGG ATTCAGACAT GGAAGATTAT TCTCCTCCTC CCAGCCTCAG TGAGGTTGCT 
CGAAAGATGA AGAAAAGAGA AAAGCAAAAG AAACGGAAAG CTTATGAACC AAAACTAACA 
CCGGAAGAAA TGATGGATTC TTCTACTTTT AAGAGGTTTA CTGCTTCAAT AGAGAATATT 
TTAGAAAATC TCGAAGACAT GGATTTTACA GCATTTGGTG ATGATGAAGA AATTCCTCAG 
GAACTGCTAT TAGGAAAACA TCAGCTTAGT GAATTGGGCA GTGAATCTGC TAAAATTAAA 
GCAATGGGAA TAATGGATAA GCTCGCAACT GATAAAACAG TAAAAGTTCT AAATATTTTG 
GAGAAAAATA TTCAGGATGG ATCTAAGCTT TCTACATTGT TAAATCATAA TAATGACACT 
GAAGAAGAGG AACGTCTGTG GAGAGACCTA ATTATGGAGA GAGTTACAAA ATCAGCAGAT 
GCTTGTCTTA CAGCTATTAA CATTATGACA TCACCTAACA TGCCAAAAGC TGTATATATT 
GAGGATGTAA TAGAAAGAGT TATACAATAC ACTAAATTTC ATTTACAAAA TACTCTTTAT 
CCTCAGTATG ATCCTGTCTA CAGAGTGGAT CCTCATGGGG GAGGAGTATT GACCTCAAAG 
GCAAAACGTG CCAAGTGTTC CACTCATAAG CAGAGGGTAA TCGTAATGCT TTATAACAAA 
GTTTGTGATA TCGTTAGCAG CTTAGCAGAA CTTTTGGAAA TACAGCTCCT AACTGATACA 
ACAATTCTTC AGATATCATC AATGGGAATA ACACCATTTT TTGTAGAAAA TGTCAGTGAA 
CTCCAGTTAT GTGCCATCAA ATTAGTAACT GCAGTATTCT CAAGGGATGA AAAACATAGG 
CAGGTGATTT TGGAAGAAAT TTTCACTTCA CTTGCAAGAT TGCCAACCAG CAAGAGGAGT 
CTAAGGAACT TCAGGTTGAA TAGTAGTGAT ATGGATGGGG AGCCTATGTA TATTCAGATG 
GTGACTGCAC TAGTTTTGCA ACTTATTCAA TGTGTAGTAC ATCTACCATC AGCAGAGAAA 
GATTCTAATT CAGAAGAGGA ATCAAACAAA AAAGTGGATC AGGATGTCGT CATTACTAAT 
TCTTATGAGA CAGCTATGAG AACTGCTCAG AATTTCCTCT CTATTTTCCT AAAAAAATGT 
GGGAGTAAGC AAGGAGAAGA AGATTACAGA CCACTCTTTG AAAATTTTGT TCAAGATCTA 
CTTTCCACAG TCAATAAGCC AGAATGGCCA GCTGCTGAAT TATTACTTAG CTTGTTAGGG 
AGACTCTTGG TTCATCAGTT CAGCAATAAG TCAACAGAGA TGGCTTTAAG AGTGGCATCT 
CTTGATTATC TTGGAACAGT AGCTGCACGA TTGAGGAAAG ATGCTGTTAC AAGCAAAATG 
GATCAAGGAT CTATCGCCCG AATTTTAAAA CAGGTTACAG GAGGGGAAGA TGAAATTCAA 
CAGTTGCAAA AGGCATTGCT AGATTACCTG GATGAAAACA CTGAAACTGA TCCTTCATTG 
GTGTTTTCTC GTAAGTTCTA TATAGCTCAG TGGTTTCGAG ACACAACTTT GGAAACAGAA 
AAAGCTATGA AATCACAGAA AGATGAGGAT TCATCAGAAG GGACACACCA TGCAAAGGAG 
GTTGAAACAA CTGGTCAAAT CATGCATCGA GCAGAAAGTC GGAAAAAATT TCTTCGAAGC 
ATTATTAAAA CTACACCTTC ACAGTTCAGT ACATTAAAGA TGAATTCTGA TACTGTGGAC 
TATGAAGATG CTTGCTTGAT TGTAAGATAC TTGGCCTCTA TGAGGCCGTT TGCCCAGAGC 
TTCGATATTT ATTTGACACA GATTCTGCGA GTACTTGGTG AAAATGCAAT AGCTGTTCGA 
ACAAAAGCCA TGAAGTGTTT ATCAGAGGTT GTTGCTGTAG ATCCCAGCAT TTTAGCAAGG 
TTGGATATGC AACGAGGTGT CCATGGAAGG TTAATGGATA ATTCCACTAG TGTCCGTGAG 
GCAGCAGTTG AATTGTTAGG TCGATTTGTC CTCTGTCGAC CTCAGCTTGC TGAACAGTAT 
TACGACATGC TGATTGAACG AATATTGGAT ACTGGTATCA GTGTCAGGAA AAGGGTAATA 
AAGATTCTGA GAGATATATG TATTGAACAA CCTACATTTC CCAAAATCAC AGAGATGTGC 
GTGAAAATGA TCCGCAGAGT AAATGATGAA GAGGGCATTA AGAAATTAGT AAATGAAACA 
TTCCAGAAAC TCTGGTTTAC TCCAACTCCT CATCATGACA AGGAAGCAAT GACCAGAAAA 
ATTTTAAACA TCACTGATGT GGTTGCAGCT TGTAGAGATA CTGGATATGA CTGGTTTGAA 
CAGCTTCTTC AAAATCTTTT AAAATCAGAA GAAGATGCTT CTTATAAACC TGTGAAGAAA 
GCTTGTACTC AGCTTGTTGA TAACTTAGTT GAGCATATCC TTAAATATGA GGAATCTCTA 
GCTGATTCTG ACAATAAGGG AGTGAATTCT GGTCGATTGG TAGCTTGCAT AACCACTTTG 
TTCTTATTCA GCAAAATCAG ACCCCAGCTA ATGGTCAAAC ATGCCATGAC CATGCAACCA 
TACCTTACCA CTAAATGTAG CACACAAAAT GATTTCATGG TCATTTGCAA TGTTGCAAAA 
ATTTTAGAAT TAGTAGTACC ATTGATGGAG CACCCAAGTG AAACCTTCCT TGCCACCATT 
GAGGAAGATC TAATGAAACT TATCATCAAA TATGGCATGA CAGTAGTGCA ACATTGTGTG 
AGCTGTCTTG GGGCTGTTGT AAATAAAGTC ACACAAAATT ATAAATTTGT GTGGGCTTGT 
TTCAATAGAT ACTATGGTGC ACTTTCAAAA TTAAAAAGTC AGCACCAAGA AGACCCAAAC 
AGTACAATAC TTACAGCAAA TAAACCAGCA CTGCTTAGAT CTCTTTTCAC TGTCGGAGCA 
CTATGTCGGC ATTTTGATTT TGATCAAGAA GACTTTAAAG GCAGCAGTAA GGTTAACATA 
AAGGATAAAG TACTTGAACT CCTGATGTAT TTTACAAAAC ATTCAGATGA GGAAGTACAA 
ACCAAAGCCA TTATTGGTCT AGGATTTGCA TTTATTCAGC ATCCAAGTCT AATGTTTGAG 
CAAGAGGTAA AGAATTTATA TAACAGCATT CTAGCTGATA AAAACAGCTC AGTAAATTTA 
AAAATCCAAG TGTTAAAAAA TCTCCAGACC TACCTACAAG AAGAAGATAC ACGTATGCAA 
CAGGCAGATA AAGACTGGAA GAAAGTAGCA AAACAGGAAG ATCTGAAAGA AATGGGTGAT 
GTGTCCTCAG GGATGAGTAG CTCCATTATG CAGCTTTACC TCAAACAAGT TCTTGAGGCT 
TTCTTTCACA CTCAGTCAAG TGTGCGCCAC TTTGCCCTAA ATGTCATTGC ATTGACTCTC 
AACCAGGGGC TCATTCATCC TGTTCAGTGT GTGCCATATT TAATTGCTAT GGGCACAGAC 
CCTGAACCTG CTATGCGGAA TAAAGCCGAC CAGCAGCTTG TGGAAATAGA CAAAAAATAT 
GCTGGATTCA TTCACATGAA AGCTGTGGCT GGTATGAAGA TGTCTTACCA AGTTCAGCAA 
GCCATCAACA CATCCCCAAA AGATGCCGTG AGGGGCTTCA GACAAGATGA ATCCTCCAGT 
GCTTTATGTT CACATCTTTA CTCCATGATC CGTGGAAATC GCCAACACAG ACGAGCCTTT 
CTAATTGCTT TACTCAATCT CTTTGATGAC ACAGCAAAAA CAGAAGTGAA TATGCTCTTG 
TATATAGCAG ACAATCTAGC CTGTTTCCCT TACCAAACAC AGGAAGAACC ATTGTTTATA 
ATGCATCATA TAGATATTAC ACTCTCAGTT TCTGGCAGTA ACCTTCTGCA GTCATTTAAG 
GAGTCTATGG TGAAGGACAA GCGGAAAGAG AAGAAACCCT CACCAAATCG GGAAAGTGCA 
TCTGGAAGTG ATAGTGAAGG AGAGGTTTCC AAGCCTCGAA AGTCACGTAA ACGAGTTGAC 
TCTGATTCAG ATTCAGATGA TGAAGATGAC ATAAATGCTG TGATGAAATG TTTACCAGAT 
AATTCAAGTC CTTTAATTGA GTTTGCAAAT GTGTCCCAGG GTATTTTATT ACTTCTGATG 
TTAAAACAAC ATTTAAAGAA CCTATGTGGA TTCTCTGATA GTAAAATCCA GAAGTATTCT 
CCATCTGAAT CTGCAAAAGT ATATGATAAA GCGATAAACC GGAAAACAGG AATCCATTTT 
CATCCTAAAC AAACCTTAGA CTTCCTGCGG AGTGATATGG CTAATTCCAA AATTACAGAA 
GATGTGAAAA GGAGTATAGT TAAACAATAT CTAGATTTCA AGCTCCTCAT GGAACATCTG 
GATCCTGATG AAGAAGAAGA AGAAGGGGAG GTGTCAGCTA GCACAAATGC TCGGAACAAA 
GCAATTACCT CACTGCTTGG AGGAGGCAGC CCTAAAAATA ATGCAGCTGA GACAGAAGAT 
GATGAAAGTG ATGGGGAGGA TAGAGGAGGA GGCACTTCAG GGTCATTGAG AAGGTCAAAA 
CGAAATTCAG ACTCTACAGA GTTGGCAGCA CAGATGAATG AAAGTGTTGA TGTCATGGAT 
GTCATCGCTA TTTGCTGTCC AAAGTACAAA GATCGACCAC AAATTGCAAG AGTAGTACAG 
AAAACCAGCA ATGGCTTCAG TGTTCAGTGG ATGGCAGGCT CCTACAGTGG CTCCTGGACT 
GAGGCTAAGC GCCGTGATGG TCGCAAACTG GTGCCTTGGG TAGACACTAT TAAAGAGTCA 
GACATTATTT ACAAAAAAAT TGCTCTAACG AGTGCTAATA AGCTGACTAA TAAAGTTGTT 
CAGACTTTAC GATCGCTGTA TGCCGCCAAG GATGGGACTT CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_007487441.1 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007487503.1 Monodelphis domestica NIPBL, cohesin loading factor (NIPBL), transcript variant X1, mRNA.

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CloneID	OMc15944
Clone ID Related Accession (Same CDS sequence)	XM_007487441.1
Accession Version	XM_007487441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	nipped-B-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCTTGA ATCAGTTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
TTGTTTAATG GACGAATAGC AGAAGAGGTA AACTGCCTTT TGGCCTGTAG GGATGAAAAT 
TTGGTTTCAC AGCTTGTTCA CACCCTCAAC CAGGTGTCAA CAGATCATAT AGAGTTGAAA 
GATAATCTTG GTAGTGATGA TCCAGAAGGA GACATACCGG TCTTACTGCA GTCTGTCCTG 
GCAAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATACGT ACAAAGTGGA 
ATGATGATGT CCCAGTATAA ACTTTCTCAA AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCTTCCAGCC GATTTGTGCC CCCACAGACA 
AGCTCTGGGA ACAGATATAT GGCACAACCA AACAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAGAGCCCTG CAGGATACAT GCCATACTCA CATCCTCCAA GTTACACACC ACATCCACAG 
ATGCAGCAAG CATCAGTATC AAGTCCCATT GTCCCAAGTG GGCTGAGAAA CATGCATGAT 
AATAAAGTTT CTGGTCAGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA TGACGGGGAT 
TCTTCAACAA TGAGGAATGC GGCATCTTTT CCTTTGAGAT CTCCACAGCC AGTGTGCTCC 
CCAGCTGGAA GTGATGGAAC TCCTAAAGGA TCAAGACCAC CATTAATCTT ACAGTCTCAG 
CCTCCACCCT ATTCATCACC TAGAGATGTT CCTCCTGACA TATTATTAGA TTCACCAGAA 
AGAAAACAGA AGAAACAAAA GAAAATGAAG TTAGGGAAAG ATGAAAAAGA TCAGAGTGAA 
AAAGCTGCAA TGTATGATAT CATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGATC TTCAGATATG GACCAGCAGG AAGATATGCT TTCTGGAATA 
GAAAATAGTA ATGTGTCAGA GAGTGATATT CCTTTTAATG TCCAGTACCC AGGACAAACT 
TCAAAAACAC CCATTACGCC GCAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
CCGCAGCATG AACAGACAGC ATTCCTTCAG GCACAACAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTA CTGCAAAACA ACCTCAGACT TCTGTGGTAC AGTCTCAGCA ACAGGCACCA 
CAACAAGGAA CGCTATATGA TGAAGTAGAA TTGGATGCAT TGGCTGAAAT CGAGAGGATA 
GAACGGGAAT CAGCTATTGA AAGGGAACGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACTGGAGGT 
AATAGACCAG CTTCTCAGGA GACAGGTTCT ACAGGAAATA ATGGGTCAAG GCCAGCTTTA 
ATGGTTAGCA TTGATCTTCA TCAGGCAGGA CGAGTAGAGT CTCAGGCATC TGTAACTCAG 
GATTCAGACA CCATTAAAAA GCCTGAAGAA AGCAAACAGT GTAACGATGG GGCTATTTCT 
GTTCATCAAG AAGATATTGG AGTTCTTAAA CAAAAACTTG AAAACCATCC TGAGACCCCT 
AGGAAAAAGT CTGATACTGA GAGTGTAAAG ACTGACATGA AACAAAATGA GAACAAATTG 
ATGGAATCTA AACCAAATGA AAACAGATTG TTGGAGACAA AACTGAATGA GAGCAGACCA 
GCAGCAGAAA CTAAACAGAA TGAGAGCAGA CCAGCAGCAG AACCTAAACA GATTGAGAGC 
AGATCAACAG TAGAAACTAA GCAAACTGAG AGCAAATCAA TGCCAGAAGT TAAACAGACT 
GAGGGCAAAT CATTGGTGGA CGTTAAGCAT AATGAGAGTA AACCAGCAGC AGAAGCTAAG 
TCCAATGAGA ACAGAGCCAC AGTAGTGGAA GCAAAACAGA ATGAAAATAA ATCAACAGAA 
GCAAAACAGA CTGAAAGTAG ACCAGTGACA GAAGTTAAAC AGACTGAGAG CAAAGTAGCA 
GCAGAAGCTA AACAAAATGA GAATAAAGCA ACTGAAACCA AGCAGAATGA AAGCAGAACG 
ACAGAAACTA AACAAAATGA GAGCAAAACT GAATCAAAAC AAAATGAGAA CAAAACAACA 
GACGCAAAAC CAAATGAAAA TAAACAAAAT AATGGTAAGC AGGAAACATC CAAGGTGAGA 
CCTGAAACTC CCAAGCAGAA GGGTGAGGGC AGACCTGAAA CTCCCAAGCA GAAGGGAGAG 
GGCAGACCTG AAACTCCCAA GCAGAAGGGT GAGGGCCGCC CTGAAACTCC TAAACAGAAG 
GGTGATGGCC GCCCTGAAAC TCCCAAACAG AAGGGTGATG GCCGCCCTGA AACTCCAAGA 
CACCGGCATG ATAGGAGAGA TTCTGGAAAG TCATCTACAG AAAAGAAACC TGAAACAAGA 
CATAAACAGG ATGTTAAATC TGATTCTCCT CGAGTGAAAT CTGAAAGATC TGAAACTTCA 
AAGCAAAGAC CAGATGGACG ATCTGTTTCT GAGTCATTAA GGCGCGACCA TGATAATAAA 
CAGAAATCAG AGGATAAGAC TGAATCAGAG AGACATCGAG GAGATCAGTC CAGAATTCGA 
AGACCAGATA CATTAAGATC CTCCAATAGA AATGAGCATG ATTCTAAACA TGGCATTAAA 
TCTGATGGTT CAAAAACAGA TAAACTAGAA AGAAAACACA GACATGAATC AGGGGAGTCC 
AGGGAAAGAC TGTCTTCTGG GGAACAGAAA TCAAGACCTG ATAGTCCTCG TATTAAAGAG 
AGTAGAGGCG ATACTAGCAA AACAAGATCT GATAAATCTG GTTTTAAATC ACCAAATAGT 
AAAGATGATC GGAGGACAGA TGGTAATAAA AGCAAAGTAG ACAGTAATAA AACACACCCT 
GACAATAAAG CAGATTTTCC CAGTTATTTG TTGGGGGGCA GATCTGGTGC ATTGAAAAAT 
TTTGTCATTC CGAAAATCAA GAGAGATAAA GATGGCAATG TTACTCAGGA AACAAGGAAA 
ATGGAACTTA AGGGGGATCA GAAAGATAAA ATTGAAAAAA TGGGACTAGT TGAAGATCTA 
AATAAAGGAG CTAAGCCTGT AGTTGTCCTG CAGAAGCTAT CTTTGGATGA TGTTCAGAAA 
TTAATTAAGG ATAGAGAGGA CAGATCAAGA AGTTCTCTTA AAACTAGCAA GAATAAGCCA 
TCCAAATCAA ATAAAGGTAG TATAGATCAG TCAGTGTTAA AAGAATTACC CCCTGAACTC 
CTGGCAGAAA TTGAATCTAC CATGCCACTT TGTGAACGTG TGAAAATGAA CAAGCGCAAA 
CGTAGCACAG TTAATGAAAA GCCAAAATAT GCTGAAATCA GTTCGGATGA AGACAATGAC 
AGTGAAGATG CTTTTGAATC TTCTCGGAAA CGACATAAGA AGGACAAAGA TGATGATAAA 
GCTTGGGAAT ATGAAGAGCG TGACAGACGA AGCTCTGGGG ATCATAGGAG AAGTGGCCAT 
TCTCATGAAG GCAGGAGGAG TTCCGGTGGT GGCCGTTATC GAAACCGCAG CCCATCGGAT 
TCAGACATGG AAGATTATTC TCCTCCTCCC AGCCTCAGTG AGGTTGCTCG AAAGATGAAG 
AAAAGAGAAA AGCAAAAGAA ACGGAAAGCT TATGAACCAA AACTAACACC GGAAGAAATG 
ATGGATTCTT CTACTTTTAA GAGGTTTACT GCTTCAATAG AGAATATTTT AGAAAATCTC 
GAAGACATGG ATTTTACAGC ATTTGGTGAT GATGAAGAAA TTCCTCAGGA ACTGCTATTA 
GGAAAACATC AGCTTAGTGA ATTGGGCAGT GAATCTGCTA AAATTAAAGC AATGGGAATA 
ATGGATAAGC TCGCAACTGA TAAAACAGTA AAAGTTCTAA ATATTTTGGA GAAAAATATT 
CAGGATGGAT CTAAGCTTTC TACATTGTTA AATCATAATA ATGACACTGA AGAAGAGGAA 
CGTCTGTGGA GAGACCTAAT TATGGAGAGA GTTACAAAAT CAGCAGATGC TTGTCTTACA 
GCTATTAACA TTATGACATC ACCTAACATG CCAAAAGCTG TATATATTGA GGATGTAATA 
GAAAGAGTTA TACAATACAC TAAATTTCAT TTACAAAATA CTCTTTATCC TCAGTATGAT 
CCTGTCTACA GAGTGGATCC TCATGGGGGA GGAGTATTGA CCTCAAAGGC AAAACGTGCC 
AAGTGTTCCA CTCATAAGCA GAGGGTAATC GTAATGCTTT ATAACAAAGT TTGTGATATC 
GTTAGCAGCT TAGCAGAACT TTTGGAAATA CAGCTCCTAA CTGATACAAC AATTCTTCAG 
ATATCATCAA TGGGAATAAC ACCATTTTTT GTAGAAAATG TCAGTGAACT CCAGTTATGT 
GCCATCAAAT TAGTAACTGC AGTATTCTCA AGGGATGAAA AACATAGGCA GGTGATTTTG 
GAAGAAATTT TCACTTCACT TGCAAGATTG CCAACCAGCA AGAGGAGTCT AAGGAACTTC 
AGGTTGAATA GTAGTGATAT GGATGGGGAG CCTATGTATA TTCAGATGGT GACTGCACTA 
GTTTTGCAAC TTATTCAATG TGTAGTACAT CTACCATCAG CAGAGAAAGA TTCTAATTCA 
GAAGAGGAAT CAAACAAAAA AGTGGATCAG GATGTCGTCA TTACTAATTC TTATGAGACA 
GCTATGAGAA CTGCTCAGAA TTTCCTCTCT ATTTTCCTAA AAAAATGTGG GAGTAAGCAA 
GGAGAAGAAG ATTACAGACC ACTCTTTGAA AATTTTGTTC AAGATCTACT TTCCACAGTC 
AATAAGCCAG AATGGCCAGC TGCTGAATTA TTACTTAGCT TGTTAGGGAG ACTCTTGGTT 
CATCAGTTCA GCAATAAGTC AACAGAGATG GCTTTAAGAG TGGCATCTCT TGATTATCTT 
GGAACAGTAG CTGCACGATT GAGGAAAGAT GCTGTTACAA GCAAAATGGA TCAAGGATCT 
ATCGCCCGAA TTTTAAAACA GGTTACAGGA GGGGAAGATG AAATTCAACA GTTGCAAAAG 
GCATTGCTAG ATTACCTGGA TGAAAACACT GAAACTGATC CTTCATTGGT GTTTTCTCGT 
AAGTTCTATA TAGCTCAGTG GTTTCGAGAC ACAACTTTGG AAACAGAAAA AGCTATGAAA 
TCACAGAAAG ATGAGGATTC ATCAGAAGGG ACACACCATG CAAAGGAGGT TGAAACAACT 
GGTCAAATCA TGCATCGAGC AGAAAGTCGG AAAAAATTTC TTCGAAGCAT TATTAAAACT 
ACACCTTCAC AGTTCAGTAC ATTAAAGATG AATTCTGATA CTGTGGACTA TGAAGATGCT 
TGCTTGATTG TAAGATACTT GGCCTCTATG AGGCCGTTTG CCCAGAGCTT CGATATTTAT 
TTGACACAGA TTCTGCGAGT ACTTGGTGAA AATGCAATAG CTGTTCGAAC AAAAGCCATG 
AAGTGTTTAT CAGAGGTTGT TGCTGTAGAT CCCAGCATTT TAGCAAGGTT GGATATGCAA 
CGAGGTGTCC ATGGAAGGTT AATGGATAAT TCCACTAGTG TCCGTGAGGC AGCAGTTGAA 
TTGTTAGGTC GATTTGTCCT CTGTCGACCT CAGCTTGCTG AACAGTATTA CGACATGCTG 
ATTGAACGAA TATTGGATAC TGGTATCAGT GTCAGGAAAA GGGTAATAAA GATTCTGAGA 
GATATATGTA TTGAACAACC TACATTTCCC AAAATCACAG AGATGTGCGT GAAAATGATC 
CGCAGAGTAA ATGATGAAGA GGGCATTAAG AAATTAGTAA ATGAAACATT CCAGAAACTC 
TGGTTTACTC CAACTCCTCA TCATGACAAG GAAGCAATGA CCAGAAAAAT TTTAAACATC 
ACTGATGTGG TTGCAGCTTG TAGAGATACT GGATATGACT GGTTTGAACA GCTTCTTCAA 
AATCTTTTAA AATCAGAAGA AGATGCTTCT TATAAACCTG TGAAGAAAGC TTGTACTCAG 
CTTGTTGATA ACTTAGTTGA GCATATCCTT AAATATGAGG AATCTCTAGC TGATTCTGAC 
AATAAGGGAG TGAATTCTGG TCGATTGGTA GCTTGCATAA CCACTTTGTT CTTATTCAGC 
AAAATCAGAC CCCAGCTAAT GGTCAAACAT GCCATGACCA TGCAACCATA CCTTACCACT 
AAATGTAGCA CACAAAATGA TTTCATGGTC ATTTGCAATG TTGCAAAAAT TTTAGAATTA 
GTAGTACCAT TGATGGAGCA CCCAAGTGAA ACCTTCCTTG CCACCATTGA GGAAGATCTA 
ATGAAACTTA TCATCAAATA TGGCATGACA GTAGTGCAAC ATTGTGTGAG CTGTCTTGGG 
GCTGTTGTAA ATAAAGTCAC ACAAAATTAT AAATTTGTGT GGGCTTGTTT CAATAGATAC 
TATGGTGCAC TTTCAAAATT AAAAAGTCAG CACCAAGAAG ACCCAAACAG TACAATACTT 
ACAGCAAATA AACCAGCACT GCTTAGATCT CTTTTCACTG TCGGAGCACT ATGTCGGCAT 
TTTGATTTTG ATCAAGAAGA CTTTAAAGGC AGCAGTAAGG TTAACATAAA GGATAAAGTA 
CTTGAACTCC TGATGTATTT TACAAAACAT TCAGATGAGG AAGTACAAAC CAAAGCCATT 
ATTGGTCTAG GATTTGCATT TATTCAGCAT CCAAGTCTAA TGTTTGAGCA AGAGGTAAAG 
AATTTATATA ACAGCATTCT AGCTGATAAA AACAGCTCAG TAAATTTAAA AATCCAAGTG 
TTAAAAAATC TCCAGACCTA CCTACAAGAA GAAGATACAC GTATGCAACA GGCAGATAAA 
GACTGGAAGA AAGTAGCAAA ACAGGAAGAT CTGAAAGAAA TGGGTGATGT GTCCTCAGGG 
ATGAGTAGCT CCATTATGCA GCTTTACCTC AAACAAGTTC TTGAGGCTTT CTTTCACACT 
CAGTCAAGTG TGCGCCACTT TGCCCTAAAT GTCATTGCAT TGACTCTCAA CCAGGGGCTC 
ATTCATCCTG TTCAGTGTGT GCCATATTTA ATTGCTATGG GCACAGACCC TGAACCTGCT 
ATGCGGAATA AAGCCGACCA GCAGCTTGTG GAAATAGACA AAAAATATGC TGGATTCATT 
CACATGAAAG CTGTGGCTGG TATGAAGATG TCTTACCAAG TTCAGCAAGC CATCAACACA 
TCCCCAAAAG ATGCCGTGAG GGGCTTCAGA CAAGATGAAT CCTCCAGTGC TTTATGTTCA 
CATCTTTACT CCATGATCCG TGGAAATCGC CAACACAGAC GAGCCTTTCT AATTGCTTTA 
CTCAATCTCT TTGATGACAC AGCAAAAACA GAAGTGAATA TGCTCTTGTA TATAGCAGAC 
AATCTAGCCT GTTTCCCTTA CCAAACACAG GAAGAACCAT TGTTTATAAT GCATCATATA 
GATATTACAC TCTCAGTTTC TGGCAGTAAC CTTCTGCAGT CATTTAAGGA GTCTATGGTG 
AAGGACAAGC GGAAAGAGAA GAAACCCTCA CCAAATCGGG AAAGTGCATC TGGAAGTGAT 
AGTGAAGGAG AGGTTTCCAA GCCTCGAAAG TCACGTAAAC GAGTTGACTC TGATTCAGAT 
TCAGATGATG AAGATGACAT AAATGCTGTG ATGAAATGTT TACCAGATAA TTCAAGTCCT 
TTAATTGAGT TTGCAAATGT GTCCCAGGGT ATTTTATTAC TTCTGATGTT AAAACAACAT 
TTAAAGAACC TATGTGGATT CTCTGATAGT AAAATCCAGA AGTATTCTCC ATCTGAATCT 
GCAAAAGTAT ATGATAAAGC GATAAACCGG AAAACAGGAA TCCATTTTCA TCCTAAACAA 
ACCTTAGACT TCCTGCGGAG TGATATGGCT AATTCCAAAA TTACAGAAGA TGTGAAAAGG 
AGTATAGTTA AACAATATCT AGATTTCAAG CTCCTCATGG AACATCTGGA TCCTGATGAA 
GAAGAAGAAG AAGGGGAGGT GTCAGCTAGC ACAAATGCTC GGAACAAAGC AATTACCTCA 
CTGCTTGGAG GAGGCAGCCC TAAAAATAAT GCAGCTGAGA CAGAAGATGA TGAAAGTGAT 
GGGGAGGATA GAGGAGGAGG CACTTCAGGG TCATTGAGAA GGTCAAAACG AAATTCAGAC 
TCTACAGAGT TGGCAGCACA GATGAATGAA AGTGTTGATG TCATGGATGT CATCGCTATT 
TGCTGTCCAA AGTACAAAGA TCGACCACAA ATTGCAAGAG TAGTACAGAA AACCAGCAAT 
GGCTTCAGTG TTCAGTGGAT GGCAGGCTCC TACAGTGGCT CCTGGACTGA GGCTAAGCGC 
CGTGATGGTC GCAAACTGGT GCCTTGGGTA GACACTATTA AAGAGTCAGA CATTATTTAC 
AAAAAAATTG CTCTAACGAG TGCTAATAAG CTGACTAATA AAGTTGTTCA GACTTTACGA 
TCGCTGTATG CCGCCAAGGA TGGGACTTCC AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007487503.1
CDS	433..9168
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNGRIAEEVNCLLACRDENLVSQLVHTLNQVSTDHIELKDNLGSDDPEGDIPVLLQSVLARNPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRYMAQPNSPVPSPYAPQSPAGYMPYSHPPSYTPHPQMQQASVSSPIVPSGLRNMHDNKVSGQLSGNSANHHADNPRHGSSDDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSDGTPKGSRPPLILQSQPPPYSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKDQSEKAAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMLSGIENSNVSESDIPFNVQYPGQTSKTPITPQDVNRPLNAAQCLPQHEQTAFLQAQQVPVLQQNTSVTAKQPQTSVVQSQQQAPQQGTLYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNNGSRPALMVSIDLHQAGRVESQASVTQDSDTIKKPEESKQCNDGAISVHQEDIGVLKQKLENHPETPRKKSDTESVKTDMKQNENKLMESKPNENRLLETKLNESRPAAETKQNESRPAAEPKQIESRSTVETKQTESKSMPEVKQTEGKSLVDVKHNESKPAAEAKSNENRATVVEAKQNENKSTEAKQTESRPVTEVKQTESKVAAEAKQNENKATETKQNESRTTETKQNESKTESKQNENKTTDAKPNENKQNNGKQETSKVRPETPKQKGEGRPETPKQKGEGRPETPKQKGEGRPETPKQKGDGRPETPKQKGDGRPETPRHRHDRRDSGKSSTEKKPETRHKQDVKSDSPRVKSERSETSKQRPDGRSVSESLRRDHDNKQKSEDKTESERHRGDQSRIRRPDTLRSSNRNEHDSKHGIKSDGSKTDKLERKHRHESGESRERLSSGEQKSRPDSPRIKESRGDTSKTRSDKSGFKSPNSKDDRRTDGNKSKVDSNKTHPDNKADFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETRKMELKGDQKDKIEKMGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDRSRSSLKTSKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSEDAFESSRKRHKKDKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKREKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILENLEDMDFTAFGDDEEIPQELLLGKHQLSELGSESAKIKAMGIMDKLATDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTAINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRVDPHGGGVLTSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLAELLEIQLLTDTTILQISSMGITPFFVENVSELQLCAIKLVTAVFSRDEKHRQVILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSAEKDSNSEEESNKKVDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIARILKQVTGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEDSSEGTHHAKEVETTGQIMHRAESRKKFLRSIIKTTPSQFSTLKMNSDTVDYEDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHHDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDASYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNYKFVWACFNRYYGALSKLKSQHQEDPNSTILTANKPALLRSLFTVGALCRHFDFDQEDFKGSSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNSILADKNSSVNLKIQVLKNLQTYLQEEDTRMQQADKDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTSPKDAVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLIALLNLFDDTAKTEVNMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKEKKPSPNRESASGSDSEGEVSKPRKSRKRVDSDSDSDDEDDINAVMKCLPDNSSPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGIHFHPKQTLDFLRSDMANSKITEDVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNAAETEDDESDGEDRGGGTSGSLRRSKRNSDSTELAAQMNESVDVMDVIAICCPKYKDRPQIARVVQKTSNGFSVQWMAGSYSGSWTEAKRRDGRKLVPWVDTIKESDIIYKKIALTSANKLTNKVVQTLRSLYAAKDGTSS

Target ORF information:

RefSeq Version	XM_007487441.1
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica NIPBL, cohesin loading factor (NIPBL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007487441.1

ORF Insert Sequence:

ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCTTGA ATCAGTTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
TTGTTTAATG GACGAATAGC AGAAGAGGTA AACTGCCTTT TGGCCTGTAG GGATGAAAAT 
TTGGTTTCAC AGCTTGTTCA CACCCTCAAC CAGGTGTCAA CAGATCATAT AGAGTTGAAA 
GATAATCTTG GTAGTGATGA TCCAGAAGGA GACATACCGG TCTTACTGCA GTCTGTCCTG 
GCAAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATACGT ACAAAGTGGA 
ATGATGATGT CCCAGTATAA ACTTTCTCAA AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCTTCCAGCC GATTTGTGCC CCCACAGACA 
AGCTCTGGGA ACAGATATAT GGCACAACCA AACAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAGAGCCCTG CAGGATACAT GCCATACTCA CATCCTCCAA GTTACACACC ACATCCACAG 
ATGCAGCAAG CATCAGTATC AAGTCCCATT GTCCCAAGTG GGCTGAGAAA CATGCATGAT 
AATAAAGTTT CTGGTCAGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA TGACGGGGAT 
TCTTCAACAA TGAGGAATGC GGCATCTTTT CCTTTGAGAT CTCCACAGCC AGTGTGCTCC 
CCAGCTGGAA GTGATGGAAC TCCTAAAGGA TCAAGACCAC CATTAATCTT ACAGTCTCAG 
CCTCCACCCT ATTCATCACC TAGAGATGTT CCTCCTGACA TATTATTAGA TTCACCAGAA 
AGAAAACAGA AGAAACAAAA GAAAATGAAG TTAGGGAAAG ATGAAAAAGA TCAGAGTGAA 
AAAGCTGCAA TGTATGATAT CATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGATC TTCAGATATG GACCAGCAGG AAGATATGCT TTCTGGAATA 
GAAAATAGTA ATGTGTCAGA GAGTGATATT CCTTTTAATG TCCAGTACCC AGGACAAACT 
TCAAAAACAC CCATTACGCC GCAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
CCGCAGCATG AACAGACAGC ATTCCTTCAG GCACAACAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTA CTGCAAAACA ACCTCAGACT TCTGTGGTAC AGTCTCAGCA ACAGGCACCA 
CAACAAGGAA CGCTATATGA TGAAGTAGAA TTGGATGCAT TGGCTGAAAT CGAGAGGATA 
GAACGGGAAT CAGCTATTGA AAGGGAACGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACTGGAGGT 
AATAGACCAG CTTCTCAGGA GACAGGTTCT ACAGGAAATA ATGGGTCAAG GCCAGCTTTA 
ATGGTTAGCA TTGATCTTCA TCAGGCAGGA CGAGTAGAGT CTCAGGCATC TGTAACTCAG 
GATTCAGACA CCATTAAAAA GCCTGAAGAA AGCAAACAGT GTAACGATGG GGCTATTTCT 
GTTCATCAAG AAGATATTGG AGTTCTTAAA CAAAAACTTG AAAACCATCC TGAGACCCCT 
AGGAAAAAGT CTGATACTGA GAGTGTAAAG ACTGACATGA AACAAAATGA GAACAAATTG 
ATGGAATCTA AACCAAATGA AAACAGATTG TTGGAGACAA AACTGAATGA GAGCAGACCA 
GCAGCAGAAA CTAAACAGAA TGAGAGCAGA CCAGCAGCAG AACCTAAACA GATTGAGAGC 
AGATCAACAG TAGAAACTAA GCAAACTGAG AGCAAATCAA TGCCAGAAGT TAAACAGACT 
GAGGGCAAAT CATTGGTGGA CGTTAAGCAT AATGAGAGTA AACCAGCAGC AGAAGCTAAG 
TCCAATGAGA ACAGAGCCAC AGTAGTGGAA GCAAAACAGA ATGAAAATAA ATCAACAGAA 
GCAAAACAGA CTGAAAGTAG ACCAGTGACA GAAGTTAAAC AGACTGAGAG CAAAGTAGCA 
GCAGAAGCTA AACAAAATGA GAATAAAGCA ACTGAAACCA AGCAGAATGA AAGCAGAACG 
ACAGAAACTA AACAAAATGA GAGCAAAACT GAATCAAAAC AAAATGAGAA CAAAACAACA 
GACGCAAAAC CAAATGAAAA TAAACAAAAT AATGGTAAGC AGGAAACATC CAAGGTGAGA 
CCTGAAACTC CCAAGCAGAA GGGTGAGGGC AGACCTGAAA CTCCCAAGCA GAAGGGAGAG 
GGCAGACCTG AAACTCCCAA GCAGAAGGGT GAGGGCCGCC CTGAAACTCC TAAACAGAAG 
GGTGATGGCC GCCCTGAAAC TCCCAAACAG AAGGGTGATG GCCGCCCTGA AACTCCAAGA 
CACCGGCATG ATAGGAGAGA TTCTGGAAAG TCATCTACAG AAAAGAAACC TGAAACAAGA 
CATAAACAGG ATGTTAAATC TGATTCTCCT CGAGTGAAAT CTGAAAGATC TGAAACTTCA 
AAGCAAAGAC CAGATGGACG ATCTGTTTCT GAGTCATTAA GGCGCGACCA TGATAATAAA 
CAGAAATCAG AGGATAAGAC TGAATCAGAG AGACATCGAG GAGATCAGTC CAGAATTCGA 
AGACCAGATA CATTAAGATC CTCCAATAGA AATGAGCATG ATTCTAAACA TGGCATTAAA 
TCTGATGGTT CAAAAACAGA TAAACTAGAA AGAAAACACA GACATGAATC AGGGGAGTCC 
AGGGAAAGAC TGTCTTCTGG GGAACAGAAA TCAAGACCTG ATAGTCCTCG TATTAAAGAG 
AGTAGAGGCG ATACTAGCAA AACAAGATCT GATAAATCTG GTTTTAAATC ACCAAATAGT 
AAAGATGATC GGAGGACAGA TGGTAATAAA AGCAAAGTAG ACAGTAATAA AACACACCCT 
GACAATAAAG CAGATTTTCC CAGTTATTTG TTGGGGGGCA GATCTGGTGC ATTGAAAAAT 
TTTGTCATTC CGAAAATCAA GAGAGATAAA GATGGCAATG TTACTCAGGA AACAAGGAAA 
ATGGAACTTA AGGGGGATCA GAAAGATAAA ATTGAAAAAA TGGGACTAGT TGAAGATCTA 
AATAAAGGAG CTAAGCCTGT AGTTGTCCTG CAGAAGCTAT CTTTGGATGA TGTTCAGAAA 
TTAATTAAGG ATAGAGAGGA CAGATCAAGA AGTTCTCTTA AAACTAGCAA GAATAAGCCA 
TCCAAATCAA ATAAAGGTAG TATAGATCAG TCAGTGTTAA AAGAATTACC CCCTGAACTC 
CTGGCAGAAA TTGAATCTAC CATGCCACTT TGTGAACGTG TGAAAATGAA CAAGCGCAAA 
CGTAGCACAG TTAATGAAAA GCCAAAATAT GCTGAAATCA GTTCGGATGA AGACAATGAC 
AGTGAAGATG CTTTTGAATC TTCTCGGAAA CGACATAAGA AGGACAAAGA TGATGATAAA 
GCTTGGGAAT ATGAAGAGCG TGACAGACGA AGCTCTGGGG ATCATAGGAG AAGTGGCCAT 
TCTCATGAAG GCAGGAGGAG TTCCGGTGGT GGCCGTTATC GAAACCGCAG CCCATCGGAT 
TCAGACATGG AAGATTATTC TCCTCCTCCC AGCCTCAGTG AGGTTGCTCG AAAGATGAAG 
AAAAGAGAAA AGCAAAAGAA ACGGAAAGCT TATGAACCAA AACTAACACC GGAAGAAATG 
ATGGATTCTT CTACTTTTAA GAGGTTTACT GCTTCAATAG AGAATATTTT AGAAAATCTC 
GAAGACATGG ATTTTACAGC ATTTGGTGAT GATGAAGAAA TTCCTCAGGA ACTGCTATTA 
GGAAAACATC AGCTTAGTGA ATTGGGCAGT GAATCTGCTA AAATTAAAGC AATGGGAATA 
ATGGATAAGC TCGCAACTGA TAAAACAGTA AAAGTTCTAA ATATTTTGGA GAAAAATATT 
CAGGATGGAT CTAAGCTTTC TACATTGTTA AATCATAATA ATGACACTGA AGAAGAGGAA 
CGTCTGTGGA GAGACCTAAT TATGGAGAGA GTTACAAAAT CAGCAGATGC TTGTCTTACA 
GCTATTAACA TTATGACATC ACCTAACATG CCAAAAGCTG TATATATTGA GGATGTAATA 
GAAAGAGTTA TACAATACAC TAAATTTCAT TTACAAAATA CTCTTTATCC TCAGTATGAT 
CCTGTCTACA GAGTGGATCC TCATGGGGGA GGAGTATTGA CCTCAAAGGC AAAACGTGCC 
AAGTGTTCCA CTCATAAGCA GAGGGTAATC GTAATGCTTT ATAACAAAGT TTGTGATATC 
GTTAGCAGCT TAGCAGAACT TTTGGAAATA CAGCTCCTAA CTGATACAAC AATTCTTCAG 
ATATCATCAA TGGGAATAAC ACCATTTTTT GTAGAAAATG TCAGTGAACT CCAGTTATGT 
GCCATCAAAT TAGTAACTGC AGTATTCTCA AGGGATGAAA AACATAGGCA GGTGATTTTG 
GAAGAAATTT TCACTTCACT TGCAAGATTG CCAACCAGCA AGAGGAGTCT AAGGAACTTC 
AGGTTGAATA GTAGTGATAT GGATGGGGAG CCTATGTATA TTCAGATGGT GACTGCACTA 
GTTTTGCAAC TTATTCAATG TGTAGTACAT CTACCATCAG CAGAGAAAGA TTCTAATTCA 
GAAGAGGAAT CAAACAAAAA AGTGGATCAG GATGTCGTCA TTACTAATTC TTATGAGACA 
GCTATGAGAA CTGCTCAGAA TTTCCTCTCT ATTTTCCTAA AAAAATGTGG GAGTAAGCAA 
GGAGAAGAAG ATTACAGACC ACTCTTTGAA AATTTTGTTC AAGATCTACT TTCCACAGTC 
AATAAGCCAG AATGGCCAGC TGCTGAATTA TTACTTAGCT TGTTAGGGAG ACTCTTGGTT 
CATCAGTTCA GCAATAAGTC AACAGAGATG GCTTTAAGAG TGGCATCTCT TGATTATCTT 
GGAACAGTAG CTGCACGATT GAGGAAAGAT GCTGTTACAA GCAAAATGGA TCAAGGATCT 
ATCGCCCGAA TTTTAAAACA GGTTACAGGA GGGGAAGATG AAATTCAACA GTTGCAAAAG 
GCATTGCTAG ATTACCTGGA TGAAAACACT GAAACTGATC CTTCATTGGT GTTTTCTCGT 
AAGTTCTATA TAGCTCAGTG GTTTCGAGAC ACAACTTTGG AAACAGAAAA AGCTATGAAA 
TCACAGAAAG ATGAGGATTC ATCAGAAGGG ACACACCATG CAAAGGAGGT TGAAACAACT 
GGTCAAATCA TGCATCGAGC AGAAAGTCGG AAAAAATTTC TTCGAAGCAT TATTAAAACT 
ACACCTTCAC AGTTCAGTAC ATTAAAGATG AATTCTGATA CTGTGGACTA TGAAGATGCT 
TGCTTGATTG TAAGATACTT GGCCTCTATG AGGCCGTTTG CCCAGAGCTT CGATATTTAT 
TTGACACAGA TTCTGCGAGT ACTTGGTGAA AATGCAATAG CTGTTCGAAC AAAAGCCATG 
AAGTGTTTAT CAGAGGTTGT TGCTGTAGAT CCCAGCATTT TAGCAAGGTT GGATATGCAA 
CGAGGTGTCC ATGGAAGGTT AATGGATAAT TCCACTAGTG TCCGTGAGGC AGCAGTTGAA 
TTGTTAGGTC GATTTGTCCT CTGTCGACCT CAGCTTGCTG AACAGTATTA CGACATGCTG 
ATTGAACGAA TATTGGATAC TGGTATCAGT GTCAGGAAAA GGGTAATAAA GATTCTGAGA 
GATATATGTA TTGAACAACC TACATTTCCC AAAATCACAG AGATGTGCGT GAAAATGATC 
CGCAGAGTAA ATGATGAAGA GGGCATTAAG AAATTAGTAA ATGAAACATT CCAGAAACTC 
TGGTTTACTC CAACTCCTCA TCATGACAAG GAAGCAATGA CCAGAAAAAT TTTAAACATC 
ACTGATGTGG TTGCAGCTTG TAGAGATACT GGATATGACT GGTTTGAACA GCTTCTTCAA 
AATCTTTTAA AATCAGAAGA AGATGCTTCT TATAAACCTG TGAAGAAAGC TTGTACTCAG 
CTTGTTGATA ACTTAGTTGA GCATATCCTT AAATATGAGG AATCTCTAGC TGATTCTGAC 
AATAAGGGAG TGAATTCTGG TCGATTGGTA GCTTGCATAA CCACTTTGTT CTTATTCAGC 
AAAATCAGAC CCCAGCTAAT GGTCAAACAT GCCATGACCA TGCAACCATA CCTTACCACT 
AAATGTAGCA CACAAAATGA TTTCATGGTC ATTTGCAATG TTGCAAAAAT TTTAGAATTA 
GTAGTACCAT TGATGGAGCA CCCAAGTGAA ACCTTCCTTG CCACCATTGA GGAAGATCTA 
ATGAAACTTA TCATCAAATA TGGCATGACA GTAGTGCAAC ATTGTGTGAG CTGTCTTGGG 
GCTGTTGTAA ATAAAGTCAC ACAAAATTAT AAATTTGTGT GGGCTTGTTT CAATAGATAC 
TATGGTGCAC TTTCAAAATT AAAAAGTCAG CACCAAGAAG ACCCAAACAG TACAATACTT 
ACAGCAAATA AACCAGCACT GCTTAGATCT CTTTTCACTG TCGGAGCACT ATGTCGGCAT 
TTTGATTTTG ATCAAGAAGA CTTTAAAGGC AGCAGTAAGG TTAACATAAA GGATAAAGTA 
CTTGAACTCC TGATGTATTT TACAAAACAT TCAGATGAGG AAGTACAAAC CAAAGCCATT 
ATTGGTCTAG GATTTGCATT TATTCAGCAT CCAAGTCTAA TGTTTGAGCA AGAGGTAAAG 
AATTTATATA ACAGCATTCT AGCTGATAAA AACAGCTCAG TAAATTTAAA AATCCAAGTG 
TTAAAAAATC TCCAGACCTA CCTACAAGAA GAAGATACAC GTATGCAACA GGCAGATAAA 
GACTGGAAGA AAGTAGCAAA ACAGGAAGAT CTGAAAGAAA TGGGTGATGT GTCCTCAGGG 
ATGAGTAGCT CCATTATGCA GCTTTACCTC AAACAAGTTC TTGAGGCTTT CTTTCACACT 
CAGTCAAGTG TGCGCCACTT TGCCCTAAAT GTCATTGCAT TGACTCTCAA CCAGGGGCTC 
ATTCATCCTG TTCAGTGTGT GCCATATTTA ATTGCTATGG GCACAGACCC TGAACCTGCT 
ATGCGGAATA AAGCCGACCA GCAGCTTGTG GAAATAGACA AAAAATATGC TGGATTCATT 
CACATGAAAG CTGTGGCTGG TATGAAGATG TCTTACCAAG TTCAGCAAGC CATCAACACA 
TCCCCAAAAG ATGCCGTGAG GGGCTTCAGA CAAGATGAAT CCTCCAGTGC TTTATGTTCA 
CATCTTTACT CCATGATCCG TGGAAATCGC CAACACAGAC GAGCCTTTCT AATTGCTTTA 
CTCAATCTCT TTGATGACAC AGCAAAAACA GAAGTGAATA TGCTCTTGTA TATAGCAGAC 
AATCTAGCCT GTTTCCCTTA CCAAACACAG GAAGAACCAT TGTTTATAAT GCATCATATA 
GATATTACAC TCTCAGTTTC TGGCAGTAAC CTTCTGCAGT CATTTAAGGA GTCTATGGTG 
AAGGACAAGC GGAAAGAGAA GAAACCCTCA CCAAATCGGG AAAGTGCATC TGGAAGTGAT 
AGTGAAGGAG AGGTTTCCAA GCCTCGAAAG TCACGTAAAC GAGTTGACTC TGATTCAGAT 
TCAGATGATG AAGATGACAT AAATGCTGTG ATGAAATGTT TACCAGATAA TTCAAGTCCT 
TTAATTGAGT TTGCAAATGT GTCCCAGGGT ATTTTATTAC TTCTGATGTT AAAACAACAT 
TTAAAGAACC TATGTGGATT CTCTGATAGT AAAATCCAGA AGTATTCTCC ATCTGAATCT 
GCAAAAGTAT ATGATAAAGC GATAAACCGG AAAACAGGAA TCCATTTTCA TCCTAAACAA 
ACCTTAGACT TCCTGCGGAG TGATATGGCT AATTCCAAAA TTACAGAAGA TGTGAAAAGG 
AGTATAGTTA AACAATATCT AGATTTCAAG CTCCTCATGG AACATCTGGA TCCTGATGAA 
GAAGAAGAAG AAGGGGAGGT GTCAGCTAGC ACAAATGCTC GGAACAAAGC AATTACCTCA 
CTGCTTGGAG GAGGCAGCCC TAAAAATAAT GCAGCTGAGA CAGAAGATGA TGAAAGTGAT 
GGGGAGGATA GAGGAGGAGG CACTTCAGGG TCATTGAGAA GGTCAAAACG AAATTCAGAC 
TCTACAGAGT TGGCAGCACA GATGAATGAA AGTGTTGATG TCATGGATGT CATCGCTATT 
TGCTGTCCAA AGTACAAAGA TCGACCACAA ATTGCAAGAG TAGTACAGAA AACCAGCAAT 
GGCTTCAGTG TTCAGTGGAT GGCAGGCTCC TACAGTGGCT CCTGGACTGA GGCTAAGCGC 
CGTGATGGTC GCAAACTGGT GCCTTGGGTA GACACTATTA AAGAGTCAGA CATTATTTAC 
AAAAAAATTG CTCTAACGAG TGCTAATAAG CTGACTAATA AAGTTGTTCA GACTTTACGA 
TCGCTGTATG CCGCCAAGGA TGGGACTTCC AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NIPBL ( XM_007487443.2 ) cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum) -> XP_007487505.1 Monodelphis domestica NIPBL, cohesin loading factor (NIPBL), transcript variant X2, mRNA.

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CloneID	OMc15945
Clone ID Related Accession (Same CDS sequence)	XM_007487443.2
Accession Version	XM_007487443.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1461600000000
Organism	Monodelphis domestica(gray short-tailed opossum)
Product	nipped-B-like protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_008803.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 27, 2016 this sequence version replaced XM_007487443.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Monodelphis domestica Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCTTGA ATCAGTTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
TTGTTTAATG GACGAATAGC AGAAGAGGTA AACTGCCTTT TGGCCTGTAG GGATGAAAAT 
TTGGTTTCAC AGCTTGTTCA CACCCTCAAC CAGGTGTCAA CAGATCATAT AGAGTTGAAA 
GATAATCTTG GTAGTGATGA TCCAGAAGGA GACATACCGG TCTTACTGCA GTCTGTCCTG 
GCAAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATACGT ACAAAGTGGA 
ATGATGATGT CCCAGTATAA ACTTTCTCAA AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCTTCCAGCC GATTTGTGCC CCCACAGACA 
AGCTCTGGGA ACAGATATAT GGCACAACCA AACAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAGAGCCCTG CAGGATACAT GCCATACTCA CATCCTCCAA GTTACACACC ACATCCACAG 
ATGCAGCAAG CATCAGTATC AAGTCCCATT GTCCCAAGTG GGCTGAGAAA CATGCATGAT 
AATAAAGTTT CTGGTCAGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA TGACGGGGAT 
TCTTCAACAA TGAGGAATGC GGCATCTTTT CCTTTGAGAT CTCCACAGCC AGTGTGCTCC 
CCAGCTGGAA GTGATGGAAC TCCTAAAGGA TCAAGACCAC CATTAATCTT ACAGTCTCAG 
CCTCCACCCT ATTCATCACC TAGAGATGTT CCTCCTGACA TATTATTAGA TTCACCAGAA 
AGAAAACAGA AGAAACAAAA GAAAATGAAG TTAGGGAAAG ATGAAAAAGA TCAGAGTGAA 
AAAGCTGCAA TGTATGATAT CATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGATC TTCAGATATG GACCAGCAGG AAGATATGCT TTCTGGAATA 
GAAAATAGTA ATGTGTCAGA GAGTGATATT CCTTTTAATG TCCAGTACCC AGGACAAACT 
TCAAAAACAC CCATTACGCC GCAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
CCGCAGCATG AACAGACAGC ATTCCTTCAG GCACAACAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTA CTGCAAAACA ACCTCAGACT TCTGTGGTAC AGTCTCAGCA ACAGGCACCA 
CAACAAGGAA CGCTATATGA TGAAGTAGAA TTGGATGCAT TGGCTGAAAT CGAGAGGATA 
GAACGGGAAT CAGCTATTGA AAGGGAACGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACTGGAGGT 
AATAGACCAG CTTCTCAGGA GACAGGTTCT ACAGGAAATA ATGGGTCAAG GCCAGCTTTA 
ATGGTTAGCA TTGATCTTCA TCAGGCAGGA CGAGTAGAGT CTCAGGCATC TGTAACTCAG 
GATTCAGACA CCATTAAAAA GCCTGAAGAA AGCAAACAGT GTAACGATGG GGCTATTTCT 
GTTCATCAAG AAGATATTGG AGTTCTTAAA CAAAAACTTG AAAACCATCC TGAGACCCCT 
AGGAAAAAGT CTGATACTGA GAGTGTAAAG ACTGACATGA AACAAAATGA GAACAAATTG 
ATGGAATCTA AACCAAATGA AAACAGATTG TTGGAGACAA AACTGAATGA GAGCAGACCA 
GCAGCAGAAA CTAAACAGAA TGAGAGCAGA CCAGCAGCAG AACCTAAACA GATTGAGAGC 
AGATCAACAG TAGAAACTAA GCAAACTGAG AGCAAATCAA TGCCAGAAGT TAAACAGACT 
GAGGGCAAAT CATTGGTGGA CGTTAAGCAT AATGAGAGTA AACCAGCAGC AGAAGCTAAG 
TCCAATGAGA ACAGAGCCAC AGTAGTGGAA GCAAAACAGA ATGAAAATAA ATCAACAGAA 
GCAAAACAGA CTGAAAGTAG ACCAGTGACA GAAGTTAAAC AGACTGAGAG CAAAGTAGCA 
GCAGAAGCTA AACAAAATGA GAATAAAGCA ACTGAAACCA AGCAGAATGA AAGCAGAACG 
ACAGAAACTA AACAAAATGA GAGCAAAACT GAATCAAAAC AAAATGAGAA CAAAACAACA 
GACGCAAAAC CAAATGAAAA TAAACAAAAT AATGGTAAGC AGGAAACATC CAAGGTGAGA 
CCTGAAACTC CCAAGCAGAA GGGTGAGGGC AGACCTGAAA CTCCCAAGCA GAAGGGAGAG 
GGCAGACCTG AAACTCCCAA GCAGAAGGGT GAGGGCCGCC CTGAAACTCC TAAACAGAAG 
GGTGATGGCC GCCCTGAAAC TCCCAAACAG AAGGGTGATG GCCGCCCTGA AACTCCAAGA 
CACCGGCATG ATAGGAGAGA TTCTGGAAAG TCATCTACAG AAAAGAAACC TGAAACAAGA 
CATAAACAGG ATGTTAAATC TGATTCTCCT CGAGTGAAAT CTGAAAGATC TGAAACTTCA 
AAGCAAAGAC CAGATGGACG ATCTGTTTCT GAGTCATTAA GGCGCGACCA TGATAATAAA 
CAGAAATCAG AGGATAAGAC TGAATCAGAG AGACATCGAG GAGATCAGTC CAGAATTCGA 
AGACCAGATA CATTAAGATC CTCCAATAGA AATGAGCATG ATTCTAAACA TGGCATTAAA 
TCTGATGGTT CAAAAACAGA TAAACTAGAA AGAAAACACA GACATGAATC AGGGGAGTCC 
AGGGAAAGAC TGTCTTCTGG GGAACAGAAA TCAAGACCTG ATAGTCCTCG TATTAAAGAG 
AGTAGAGGCG ATACTAGCAA AACAAGATCT GATAAATCTG GTTTTAAATC ACCAAATAGT 
AAAGATGATC GGAGGACAGA TGGTAATAAA AGCAAAGTAG ACAGTAATAA AACACACCCT 
GACAATAAAG CAGATTTTCC CAGTTATTTG TTGGGGGGCA GATCTGGTGC ATTGAAAAAT 
TTTGTCATTC CGAAAATCAA GAGAGATAAA GATGGCAATG TTACTCAGGA AACAAGGAAA 
ATGGAACTTA AGGGGGATCA GAAAGATAAA ATTGAAAAAA TGGGACTAGT TGAAGATCTA 
AATAAAGGAG CTAAGCCTGT AGTTGTCCTG CAGAAGCTAT CTTTGGATGA TGTTCAGAAA 
TTAATTAAGG ATAGAGAGGA CAGATCAAGA AGTTCTCTTA AAACTAGCAA GAATAAGCCA 
TCCAAATCAA ATAAAGGTAG TATAGATCAG TCAGTGTTAA AAGAATTACC CCCTGAACTC 
CTGGCAGAAA TTGAATCTAC CATGCCACTT TGTGAACGTG TGAAAATGAA CAAGCGCAAA 
CGTAGCACAG TTAATGAAAA GCCAAAATAT GCTGAAATCA GTTCGGATGA AGACAATGAC 
AGTGAAGATG CTTTTGAATC TTCTCGGAAA CGACATAAGA AGGACAAAGA TGATGATAAA 
GCTTGGGAAT ATGAAGAGCG TGACAGACGA AGCTCTGGGG ATCATAGGAG AAGTGGCCAT 
TCTCATGAAG GCAGGAGGAG TTCCGGTGGT GGCCGTTATC GAAACCGCAG CCCATCGGAT 
TCAGACATGG AAGATTATTC TCCTCCTCCC AGCCTCAGTG AGGTTGCTCG AAAGATGAAG 
AAAAGAGAAA AGCAAAAGAA ACGGAAAGCT TATGAACCAA AACTAACACC GGAAGAAATG 
ATGGATTCTT CTACTTTTAA GAGGTTTACT GCTTCAATAG AGAATATTTT AGAAAATCTC 
GAAGACATGG ATTTTACAGC ATTTGGTGAT GATGAAGAAA TTCCTCAGGA ACTGCTATTA 
GGAAAACATC AGCTTAGTGA ATTGGGCAGT GAATCTGCTA AAATTAAAGC AATGGGAATA 
ATGGATAAGC TCGCAACTGA TAAAACAGTA AAAGTTCTAA ATATTTTGGA GAAAAATATT 
CAGGATGGAT CTAAGCTTTC TACATTGTTA AATCATAATA ATGACACTGA AGAAGAGGAA 
CGTCTGTGGA GAGACCTAAT TATGGAGAGA GTTACAAAAT CAGCAGATGC TTGTCTTACA 
GCTATTAACA TTATGACATC ACCTAACATG CCAAAAGCTG TATATATTGA GGATGTAATA 
GAAAGAGTTA TACAATACAC TAAATTTCAT TTACAAAATA CTCTTTATCC TCAGTATGAT 
CCTGTCTACA GAGTGGATCC TCATGGGGGA GGAGTATTGA CCTCAAAGGC AAAACGTGCC 
AAGTGTTCCA CTCATAAGCA GAGGGTAATC GTAATGCTTT ATAACAAAGT TTGTGATATC 
GTTAGCAGCT TAGCAGAACT TTTGGAAATA CAGCTCCTAA CTGATACAAC AATTCTTCAG 
ATATCATCAA TGGGAATAAC ACCATTTTTT GTAGAAAATG TCAGTGAACT CCAGTTATGT 
GCCATCAAAT TAGTAACTGC AGTATTCTCA AGGGATGAAA AACATAGGCA GGTGATTTTG 
GAAGAAATTT TCACTTCACT TGCAAGATTG CCAACCAGCA AGAGGAGTCT AAGGAACTTC 
AGGTTGAATA GTAGTGATAT GGATGGGGAG CCTATGTATA TTCAGATGGT GACTGCACTA 
GTTTTGCAAC TTATTCAATG TGTAGTACAT CTACCATCAG CAGAGAAAGA TTCTAATTCA 
GAAGAGGAAT CAAACAAAAA AGTGGATCAG GATGTCGTCA TTACTAATTC TTATGAGACA 
GCTATGAGAA CTGCTCAGAA TTTCCTCTCT ATTTTCCTAA AAAAATGTGG GAGTAAGCAA 
GGAGAAGAAG ATTACAGACC ACTCTTTGAA AATTTTGTTC AAGATCTACT TTCCACAGTC 
AATAAGCCAG AATGGCCAGC TGCTGAATTA TTACTTAGCT TGTTAGGGAG ACTCTTGGTT 
CATCAGTTCA GCAATAAGTC AACAGAGATG GCTTTAAGAG TGGCATCTCT TGATTATCTT 
GGAACAGTAG CTGCACGATT GAGGAAAGAT GCTGTTACAA GCAAAATGGA TCAAGGATCT 
ATCGCCCGAA TTTTAAAACA GGTTACAGGA GGGGAAGATG AAATTCAACA GTTGCAAAAG 
GCATTGCTAG ATTACCTGGA TGAAAACACT GAAACTGATC CTTCATTGGT GTTTTCTCGT 
AAGTTCTATA TAGCTCAGTG GTTTCGAGAC ACAACTTTGG AAACAGAAAA AGCTATGAAA 
TCACAGAAAG ATGAGGATTC ATCAGAAGGG ACACACCATG CAAAGGAGGT TGAAACAACT 
GGTCAAATCA TGCATCGAGC AGAAAGTCGG AAAAAATTTC TTCGAAGCAT TATTAAAACT 
ACACCTTCAC AGTTCAGTAC ATTAAAGATG AATTCTGATA CTGTGGACTA TGAAGATGCT 
TGCTTGATTG TAAGATACTT GGCCTCTATG AGGCCGTTTG CCCAGAGCTT CGATATTTAT 
TTGACACAGA TTCTGCGAGT ACTTGGTGAA AATGCAATAG CTGTTCGAAC AAAAGCCATG 
AAGTGTTTAT CAGAGGTTGT TGCTGTAGAT CCCAGCATTT TAGCAAGGTT GGATATGCAA 
CGAGGTGTCC ATGGAAGGTT AATGGATAAT TCCACTAGTG TCCGTGAGGC AGCAGTTGAA 
TTGTTAGGTC GATTTGTCCT CTGTCGACCT CAGCTTGCTG AACAGTATTA CGACATGCTG 
ATTGAACGAA TATTGGATAC TGGTATCAGT GTCAGGAAAA GGGTAATAAA GATTCTGAGA 
GATATATGTA TTGAACAACC TACATTTCCC AAAATCACAG AGATGTGCGT GAAAATGATC 
CGCAGAGTAA ATGATGAAGA GGGCATTAAG AAATTAGTAA ATGAAACATT CCAGAAACTC 
TGGTTTACTC CAACTCCTCA TCATGACAAG GAAGCAATGA CCAGAAAAAT TTTAAACATC 
ACTGATGTGG TTGCAGCTTG TAGAGATACT GGATATGACT GGTTTGAACA GCTTCTTCAA 
AATCTTTTAA AATCAGAAGA AGATGCTTCT TATAAACCTG TGAAGAAAGC TTGTACTCAG 
CTTGTTGATA ACTTAGTTGA GCATATCCTT AAATATGAGG AATCTCTAGC TGATTCTGAC 
AATAAGGGAG TGAATTCTGG TCGATTGGTA GCTTGCATAA CCACTTTGTT CTTATTCAGC 
AAAATCAGAC CCCAGCTAAT GGTCAAACAT GCCATGACCA TGCAACCATA CCTTACCACT 
AAATGTAGCA CACAAAATGA TTTCATGGTC ATTTGCAATG TTGCAAAAAT TTTAGAATTA 
GTAGTACCAT TGATGGAGCA CCCAAGTGAA ACCTTCCTTG CCACCATTGA GGAAGATCTA 
ATGAAACTTA TCATCAAATA TGGCATGACA GTAGTGCAAC ATTGTGTGAG CTGTCTTGGG 
GCTGTTGTAA ATAAAGTCAC ACAAAATTAT AAATTTGTGT GGGCTTGTTT CAATAGATAC 
TATGGTGCAC TTTCAAAATT AAAAAGTCAG CACCAAGAAG ACCCAAACAG TACAATACTT 
ACAGCAAATA AACCAGCACT GCTTAGATCT CTTTTCACTG TCGGAGCACT ATGTCGGCAT 
TTTGATTTTG ATCAAGAAGA CTTTAAAGGC AGCAGTAAGG TTAACATAAA GGATAAAGTA 
CTTGAACTCC TGATGTATTT TACAAAACAT TCAGATGAGG AAGTACAAAC CAAAGCCATT 
ATTGGTCTAG GATTTGCATT TATTCAGCAT CCAAGTCTAA TGTTTGAGCA AGAGGTAAAG 
AATTTATATA ACAGCATTCT AGCTGATAAA AACAGCTCAG TAAATTTAAA AATCCAAGTG 
TTAAAAAATC TCCAGACCTA CCTACAAGAA GAAGATACAC GTATGCAACA GGCAGATAAA 
GACTGGAAGA AAGTAGCAAA ACAGGAAGAT CTGAAAGAAA TGGGTGATGT GTCCTCAGGG 
ATGAGTAGCT CCATTATGCA GCTTTACCTC AAACAAGTTC TTGAGGCTTT CTTTCACACT 
CAGTCAAGTG TGCGCCACTT TGCCCTAAAT GTCATTGCAT TGACTCTCAA CCAGGGGCTC 
ATTCATCCTG TTCAGTGTGT GCCATATTTA ATTGCTATGG GCACAGACCC TGAACCTGCT 
ATGCGGAATA AAGCCGACCA GCAGCTTGTG GAAATAGACA AAAAATATGC TGGATTCATT 
CACATGAAAG CTGTGGCTGG TATGAAGATG TCTTACCAAG TTCAGCAAGC CATCAACACA 
TCCCCAAAAG ATGCCGTGAG GGGCTTCAGA CAAGATGAAT CCTCCAGTGC TTTATGTTCA 
CATCTTTACT CCATGATCCG TGGAAATCGC CAACACAGAC GAGCCTTTCT AATTGCTTTA 
CTCAATCTCT TTGATGACAC AGCAAAAACA GAAGTGAATA TGCTCTTGTA TATAGCAGAC 
AATCTAGCCT GTTTCCCTTA CCAAACACAG GAAGAACCAT TGTTTATAAT GCATCATATA 
GATATTACAC TCTCAGTTTC TGGCAGTAAC CTTCTGCAGT CATTTAAGGA GTCTATGGTG 
AAGGACAAGC GGAAAGAGAA GAAACCCTCA CCAAATCGGG AAAGTGCATC TGGAAGTGAT 
AGTGAAGGAG AGGTTTCCAA GCCTCGAAAG TCACGTAAAC GAGTTGACTC TGATTCAGAT 
TCAGATGATG AAGATGACAT AAATGCTGTG ATGAAATGTT TACCAGATAA TTCAAGTCCT 
TTAATTGAGT TTGCAAATGT GTCCCAGGGT ATTTTATTAC TTCTGATGTT AAAACAACAT 
TTAAAGAACC TATGTGGATT CTCTGATAGT AAAATCCAGA AGTATTCTCC ATCTGAATCT 
GCAAAAGTAT ATGATAAAGC GATAAACCGG AAAACAGGAA TCCATTTTCA TCCTAAACAA 
ACCTTAGACT TCCTGCGGAG TGATATGGCT AATTCCAAAA TTACAGAAGA TGTGAAAAGG 
AGTATAGTTA AACAATATCT AGATTTCAAG CTCCTCATGG AACATCTGGA TCCTGATGAA 
GAAGAAGAAG AAGGGGAGGT GTCAGCTAGC ACAAATGCTC GGAACAAAGC AATTACCTCA 
CTGCTTGGAG GAGGCAGCCC TAAAAATAAT GCAGCTGAGA CAGAAGATGA TGAAAGTGAT 
GGGGAGGATA GAGGAGGAGG CACTTCAGGG GTGAGGCGGA GGAGGAGTCA ACGTATTTCG 
CAGCGTATTA CTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007487505.1
CDS	433..8850
Translation	MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNGRIAEEVNCLLACRDENLVSQLVHTLNQVSTDHIELKDNLGSDDPEGDIPVLLQSVLARNPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASSNYQQTTISHSPSSRFVPPQTSSGNRYMAQPNSPVPSPYAPQSPAGYMPYSHPPSYTPHPQMQQASVSSPIVPSGLRNMHDNKVSGQLSGNSANHHADNPRHGSSDDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCSPAGSDGTPKGSRPPLILQSQPPPYSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKDQSEKAAMYDIISSPSKDSTKLTLRLSRVRSSDMDQQEDMLSGIENSNVSESDIPFNVQYPGQTSKTPITPQDVNRPLNAAQCLPQHEQTAFLQAQQVPVLQQNTSVTAKQPQTSVVQSQQQAPQQGTLYDEVELDALAEIERIERESAIERERFSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNNGSRPALMVSIDLHQAGRVESQASVTQDSDTIKKPEESKQCNDGAISVHQEDIGVLKQKLENHPETPRKKSDTESVKTDMKQNENKLMESKPNENRLLETKLNESRPAAETKQNESRPAAEPKQIESRSTVETKQTESKSMPEVKQTEGKSLVDVKHNESKPAAEAKSNENRATVVEAKQNENKSTEAKQTESRPVTEVKQTESKVAAEAKQNENKATETKQNESRTTETKQNESKTESKQNENKTTDAKPNENKQNNGKQETSKVRPETPKQKGEGRPETPKQKGEGRPETPKQKGEGRPETPKQKGDGRPETPKQKGDGRPETPRHRHDRRDSGKSSTEKKPETRHKQDVKSDSPRVKSERSETSKQRPDGRSVSESLRRDHDNKQKSEDKTESERHRGDQSRIRRPDTLRSSNRNEHDSKHGIKSDGSKTDKLERKHRHESGESRERLSSGEQKSRPDSPRIKESRGDTSKTRSDKSGFKSPNSKDDRRTDGNKSKVDSNKTHPDNKADFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETRKMELKGDQKDKIEKMGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDRSRSSLKTSKNKPSKSNKGSIDQSVLKELPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSEDAFESSRKRHKKDKDDDKAWEYEERDRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKREKQKKRKAYEPKLTPEEMMDSSTFKRFTASIENILENLEDMDFTAFGDDEEIPQELLLGKHQLSELGSESAKIKAMGIMDKLATDKTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTAINIMTSPNMPKAVYIEDVIERVIQYTKFHLQNTLYPQYDPVYRVDPHGGGVLTSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLAELLEIQLLTDTTILQISSMGITPFFVENVSELQLCAIKLVTAVFSRDEKHRQVILEEIFTSLARLPTSKRSLRNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSAEKDSNSEEESNKKVDQDVVITNSYETAMRTAQNFLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASLDYLGTVAARLRKDAVTSKMDQGSIARILKQVTGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQWFRDTTLETEKAMKSQKDEDSSEGTHHAKEVETTGQIMHRAESRKKFLRSIIKTTPSQFSTLKMNSDTVDYEDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRLMDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCVKMIRRVNDEEGIKKLVNETFQKLWFTPTPHHDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEEDASYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPYLTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVTQNYKFVWACFNRYYGALSKLKSQHQEDPNSTILTANKPALLRSLFTVGALCRHFDFDQEDFKGSSKVNIKDKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNSILADKNSSVNLKIQVLKNLQTYLQEEDTRMQQADKDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLNQGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTSPKDAVRGFRQDESSSALCSHLYSMIRGNRQHRRAFLIALLNLFDDTAKTEVNMLLYIADNLACFPYQTQEEPLFIMHHIDITLSVSGSNLLQSFKESMVKDKRKEKKPSPNRESASGSDSEGEVSKPRKSRKRVDSDSDSDDEDDINAVMKCLPDNSSPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGIHFHPKQTLDFLRSDMANSKITEDVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPKNNAAETEDDESDGEDRGGGTSGVRRRRSQRISQRITH

Target ORF information:

RefSeq Version	XM_007487443.2
Organism	Monodelphis domestica(gray short-tailed opossum)
Definition	Monodelphis domestica NIPBL, cohesin loading factor (NIPBL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007487443.2

ORF Insert Sequence:

ATGAATGGGG ATATGCCTCA TGTTCCCATC ACTACTCTTG CGGGGATTGC TAGTCTCACA 
GACCTCTTGA ATCAGTTGCC TCTTCCATCT CCTTTACCTG CTACAACTAC AAAGAGCCTT 
TTGTTTAATG GACGAATAGC AGAAGAGGTA AACTGCCTTT TGGCCTGTAG GGATGAAAAT 
TTGGTTTCAC AGCTTGTTCA CACCCTCAAC CAGGTGTCAA CAGATCATAT AGAGTTGAAA 
GATAATCTTG GTAGTGATGA TCCAGAAGGA GACATACCGG TCTTACTGCA GTCTGTCCTG 
GCAAGGAATC CTAATGTTTT CAGGGAGAAA AGCATGCAGA ACAGATACGT ACAAAGTGGA 
ATGATGATGT CCCAGTATAA ACTTTCTCAA AATTCCATGC ACAGTAGTCC TGCATCTTCC 
AATTATCAAC AAACCACTAT CTCACATAGC CCTTCCAGCC GATTTGTGCC CCCACAGACA 
AGCTCTGGGA ACAGATATAT GGCACAACCA AACAGCCCAG TGCCTAGTCC ATATGCCCCA 
CAGAGCCCTG CAGGATACAT GCCATACTCA CATCCTCCAA GTTACACACC ACATCCACAG 
ATGCAGCAAG CATCAGTATC AAGTCCCATT GTCCCAAGTG GGCTGAGAAA CATGCATGAT 
AATAAAGTTT CTGGTCAGTT GTCTGGCAAT TCAGCTAATC ATCATGCTGA TAATCCTAGA 
CATGGCTCAA GTGACGACTA CCTACACATG GTGCACAGGC TAAGTAGTGA TGACGGGGAT 
TCTTCAACAA TGAGGAATGC GGCATCTTTT CCTTTGAGAT CTCCACAGCC AGTGTGCTCC 
CCAGCTGGAA GTGATGGAAC TCCTAAAGGA TCAAGACCAC CATTAATCTT ACAGTCTCAG 
CCTCCACCCT ATTCATCACC TAGAGATGTT CCTCCTGACA TATTATTAGA TTCACCAGAA 
AGAAAACAGA AGAAACAAAA GAAAATGAAG TTAGGGAAAG ATGAAAAAGA TCAGAGTGAA 
AAAGCTGCAA TGTATGATAT CATTAGTTCT CCATCCAAGG ACTCTACTAA ACTTACATTA 
AGACTTTCTC GTGTAAGATC TTCAGATATG GACCAGCAGG AAGATATGCT TTCTGGAATA 
GAAAATAGTA ATGTGTCAGA GAGTGATATT CCTTTTAATG TCCAGTACCC AGGACAAACT 
TCAAAAACAC CCATTACGCC GCAGGATGTA AACCGCCCAC TAAATGCTGC TCAGTGTTTG 
CCGCAGCATG AACAGACAGC ATTCCTTCAG GCACAACAAG TGCCTGTTTT ACAACAGAAC 
ACTTCAGTTA CTGCAAAACA ACCTCAGACT TCTGTGGTAC AGTCTCAGCA ACAGGCACCA 
CAACAAGGAA CGCTATATGA TGAAGTAGAA TTGGATGCAT TGGCTGAAAT CGAGAGGATA 
GAACGGGAAT CAGCTATTGA AAGGGAACGT TTTTCAAAAG AAGTTCAAGA TAAAGATAAG 
CCTTTGAAAA AAAGAAAACA AGATTCTTAC CCACAGGAGG CTGGGGGTGC TACTGGAGGT 
AATAGACCAG CTTCTCAGGA GACAGGTTCT ACAGGAAATA ATGGGTCAAG GCCAGCTTTA 
ATGGTTAGCA TTGATCTTCA TCAGGCAGGA CGAGTAGAGT CTCAGGCATC TGTAACTCAG 
GATTCAGACA CCATTAAAAA GCCTGAAGAA AGCAAACAGT GTAACGATGG GGCTATTTCT 
GTTCATCAAG AAGATATTGG AGTTCTTAAA CAAAAACTTG AAAACCATCC TGAGACCCCT 
AGGAAAAAGT CTGATACTGA GAGTGTAAAG ACTGACATGA AACAAAATGA GAACAAATTG 
ATGGAATCTA AACCAAATGA AAACAGATTG TTGGAGACAA AACTGAATGA GAGCAGACCA 
GCAGCAGAAA CTAAACAGAA TGAGAGCAGA CCAGCAGCAG AACCTAAACA GATTGAGAGC 
AGATCAACAG TAGAAACTAA GCAAACTGAG AGCAAATCAA TGCCAGAAGT TAAACAGACT 
GAGGGCAAAT CATTGGTGGA CGTTAAGCAT AATGAGAGTA AACCAGCAGC AGAAGCTAAG 
TCCAATGAGA ACAGAGCCAC AGTAGTGGAA GCAAAACAGA ATGAAAATAA ATCAACAGAA 
GCAAAACAGA CTGAAAGTAG ACCAGTGACA GAAGTTAAAC AGACTGAGAG CAAAGTAGCA 
GCAGAAGCTA AACAAAATGA GAATAAAGCA ACTGAAACCA AGCAGAATGA AAGCAGAACG 
ACAGAAACTA AACAAAATGA GAGCAAAACT GAATCAAAAC AAAATGAGAA CAAAACAACA 
GACGCAAAAC CAAATGAAAA TAAACAAAAT AATGGTAAGC AGGAAACATC CAAGGTGAGA 
CCTGAAACTC CCAAGCAGAA GGGTGAGGGC AGACCTGAAA CTCCCAAGCA GAAGGGAGAG 
GGCAGACCTG AAACTCCCAA GCAGAAGGGT GAGGGCCGCC CTGAAACTCC TAAACAGAAG 
GGTGATGGCC GCCCTGAAAC TCCCAAACAG AAGGGTGATG GCCGCCCTGA AACTCCAAGA 
CACCGGCATG ATAGGAGAGA TTCTGGAAAG TCATCTACAG AAAAGAAACC TGAAACAAGA 
CATAAACAGG ATGTTAAATC TGATTCTCCT CGAGTGAAAT CTGAAAGATC TGAAACTTCA 
AAGCAAAGAC CAGATGGACG ATCTGTTTCT GAGTCATTAA GGCGCGACCA TGATAATAAA 
CAGAAATCAG AGGATAAGAC TGAATCAGAG AGACATCGAG GAGATCAGTC CAGAATTCGA 
AGACCAGATA CATTAAGATC CTCCAATAGA AATGAGCATG ATTCTAAACA TGGCATTAAA 
TCTGATGGTT CAAAAACAGA TAAACTAGAA AGAAAACACA GACATGAATC AGGGGAGTCC 
AGGGAAAGAC TGTCTTCTGG GGAACAGAAA TCAAGACCTG ATAGTCCTCG TATTAAAGAG 
AGTAGAGGCG ATACTAGCAA AACAAGATCT GATAAATCTG GTTTTAAATC ACCAAATAGT 
AAAGATGATC GGAGGACAGA TGGTAATAAA AGCAAAGTAG ACAGTAATAA AACACACCCT 
GACAATAAAG CAGATTTTCC CAGTTATTTG TTGGGGGGCA GATCTGGTGC ATTGAAAAAT 
TTTGTCATTC CGAAAATCAA GAGAGATAAA GATGGCAATG TTACTCAGGA AACAAGGAAA 
ATGGAACTTA AGGGGGATCA GAAAGATAAA ATTGAAAAAA TGGGACTAGT TGAAGATCTA 
AATAAAGGAG CTAAGCCTGT AGTTGTCCTG CAGAAGCTAT CTTTGGATGA TGTTCAGAAA 
TTAATTAAGG ATAGAGAGGA CAGATCAAGA AGTTCTCTTA AAACTAGCAA GAATAAGCCA 
TCCAAATCAA ATAAAGGTAG TATAGATCAG TCAGTGTTAA AAGAATTACC CCCTGAACTC 
CTGGCAGAAA TTGAATCTAC CATGCCACTT TGTGAACGTG TGAAAATGAA CAAGCGCAAA 
CGTAGCACAG TTAATGAAAA GCCAAAATAT GCTGAAATCA GTTCGGATGA AGACAATGAC 
AGTGAAGATG CTTTTGAATC TTCTCGGAAA CGACATAAGA AGGACAAAGA TGATGATAAA 
GCTTGGGAAT ATGAAGAGCG TGACAGACGA AGCTCTGGGG ATCATAGGAG AAGTGGCCAT 
TCTCATGAAG GCAGGAGGAG TTCCGGTGGT GGCCGTTATC GAAACCGCAG CCCATCGGAT 
TCAGACATGG AAGATTATTC TCCTCCTCCC AGCCTCAGTG AGGTTGCTCG AAAGATGAAG 
AAAAGAGAAA AGCAAAAGAA ACGGAAAGCT TATGAACCAA AACTAACACC GGAAGAAATG 
ATGGATTCTT CTACTTTTAA GAGGTTTACT GCTTCAATAG AGAATATTTT AGAAAATCTC 
GAAGACATGG ATTTTACAGC ATTTGGTGAT GATGAAGAAA TTCCTCAGGA ACTGCTATTA 
GGAAAACATC AGCTTAGTGA ATTGGGCAGT GAATCTGCTA AAATTAAAGC AATGGGAATA 
ATGGATAAGC TCGCAACTGA TAAAACAGTA AAAGTTCTAA ATATTTTGGA GAAAAATATT 
CAGGATGGAT CTAAGCTTTC TACATTGTTA AATCATAATA ATGACACTGA AGAAGAGGAA 
CGTCTGTGGA GAGACCTAAT TATGGAGAGA GTTACAAAAT CAGCAGATGC TTGTCTTACA 
GCTATTAACA TTATGACATC ACCTAACATG CCAAAAGCTG TATATATTGA GGATGTAATA 
GAAAGAGTTA TACAATACAC TAAATTTCAT TTACAAAATA CTCTTTATCC TCAGTATGAT 
CCTGTCTACA GAGTGGATCC TCATGGGGGA GGAGTATTGA CCTCAAAGGC AAAACGTGCC 
AAGTGTTCCA CTCATAAGCA GAGGGTAATC GTAATGCTTT ATAACAAAGT TTGTGATATC 
GTTAGCAGCT TAGCAGAACT TTTGGAAATA CAGCTCCTAA CTGATACAAC AATTCTTCAG 
ATATCATCAA TGGGAATAAC ACCATTTTTT GTAGAAAATG TCAGTGAACT CCAGTTATGT 
GCCATCAAAT TAGTAACTGC AGTATTCTCA AGGGATGAAA AACATAGGCA GGTGATTTTG 
GAAGAAATTT TCACTTCACT TGCAAGATTG CCAACCAGCA AGAGGAGTCT AAGGAACTTC 
AGGTTGAATA GTAGTGATAT GGATGGGGAG CCTATGTATA TTCAGATGGT GACTGCACTA 
GTTTTGCAAC TTATTCAATG TGTAGTACAT CTACCATCAG CAGAGAAAGA TTCTAATTCA 
GAAGAGGAAT CAAACAAAAA AGTGGATCAG GATGTCGTCA TTACTAATTC TTATGAGACA 
GCTATGAGAA CTGCTCAGAA TTTCCTCTCT ATTTTCCTAA AAAAATGTGG GAGTAAGCAA 
GGAGAAGAAG ATTACAGACC ACTCTTTGAA AATTTTGTTC AAGATCTACT TTCCACAGTC 
AATAAGCCAG AATGGCCAGC TGCTGAATTA TTACTTAGCT TGTTAGGGAG ACTCTTGGTT 
CATCAGTTCA GCAATAAGTC AACAGAGATG GCTTTAAGAG TGGCATCTCT TGATTATCTT 
GGAACAGTAG CTGCACGATT GAGGAAAGAT GCTGTTACAA GCAAAATGGA TCAAGGATCT 
ATCGCCCGAA TTTTAAAACA GGTTACAGGA GGGGAAGATG AAATTCAACA GTTGCAAAAG 
GCATTGCTAG ATTACCTGGA TGAAAACACT GAAACTGATC CTTCATTGGT GTTTTCTCGT 
AAGTTCTATA TAGCTCAGTG GTTTCGAGAC ACAACTTTGG AAACAGAAAA AGCTATGAAA 
TCACAGAAAG ATGAGGATTC ATCAGAAGGG ACACACCATG CAAAGGAGGT TGAAACAACT 
GGTCAAATCA TGCATCGAGC AGAAAGTCGG AAAAAATTTC TTCGAAGCAT TATTAAAACT 
ACACCTTCAC AGTTCAGTAC ATTAAAGATG AATTCTGATA CTGTGGACTA TGAAGATGCT 
TGCTTGATTG TAAGATACTT GGCCTCTATG AGGCCGTTTG CCCAGAGCTT CGATATTTAT 
TTGACACAGA TTCTGCGAGT ACTTGGTGAA AATGCAATAG CTGTTCGAAC AAAAGCCATG 
AAGTGTTTAT CAGAGGTTGT TGCTGTAGAT CCCAGCATTT TAGCAAGGTT GGATATGCAA 
CGAGGTGTCC ATGGAAGGTT AATGGATAAT TCCACTAGTG TCCGTGAGGC AGCAGTTGAA 
TTGTTAGGTC GATTTGTCCT CTGTCGACCT CAGCTTGCTG AACAGTATTA CGACATGCTG 
ATTGAACGAA TATTGGATAC TGGTATCAGT GTCAGGAAAA GGGTAATAAA GATTCTGAGA 
GATATATGTA TTGAACAACC TACATTTCCC AAAATCACAG AGATGTGCGT GAAAATGATC 
CGCAGAGTAA ATGATGAAGA GGGCATTAAG AAATTAGTAA ATGAAACATT CCAGAAACTC 
TGGTTTACTC CAACTCCTCA TCATGACAAG GAAGCAATGA CCAGAAAAAT TTTAAACATC 
ACTGATGTGG TTGCAGCTTG TAGAGATACT GGATATGACT GGTTTGAACA GCTTCTTCAA 
AATCTTTTAA AATCAGAAGA AGATGCTTCT TATAAACCTG TGAAGAAAGC TTGTACTCAG 
CTTGTTGATA ACTTAGTTGA GCATATCCTT AAATATGAGG AATCTCTAGC TGATTCTGAC 
AATAAGGGAG TGAATTCTGG TCGATTGGTA GCTTGCATAA CCACTTTGTT CTTATTCAGC 
AAAATCAGAC CCCAGCTAAT GGTCAAACAT GCCATGACCA TGCAACCATA CCTTACCACT 
AAATGTAGCA CACAAAATGA TTTCATGGTC ATTTGCAATG TTGCAAAAAT TTTAGAATTA 
GTAGTACCAT TGATGGAGCA CCCAAGTGAA ACCTTCCTTG CCACCATTGA GGAAGATCTA 
ATGAAACTTA TCATCAAATA TGGCATGACA GTAGTGCAAC ATTGTGTGAG CTGTCTTGGG 
GCTGTTGTAA ATAAAGTCAC ACAAAATTAT AAATTTGTGT GGGCTTGTTT CAATAGATAC 
TATGGTGCAC TTTCAAAATT AAAAAGTCAG CACCAAGAAG ACCCAAACAG TACAATACTT 
ACAGCAAATA AACCAGCACT GCTTAGATCT CTTTTCACTG TCGGAGCACT ATGTCGGCAT 
TTTGATTTTG ATCAAGAAGA CTTTAAAGGC AGCAGTAAGG TTAACATAAA GGATAAAGTA 
CTTGAACTCC TGATGTATTT TACAAAACAT TCAGATGAGG AAGTACAAAC CAAAGCCATT 
ATTGGTCTAG GATTTGCATT TATTCAGCAT CCAAGTCTAA TGTTTGAGCA AGAGGTAAAG 
AATTTATATA ACAGCATTCT AGCTGATAAA AACAGCTCAG TAAATTTAAA AATCCAAGTG 
TTAAAAAATC TCCAGACCTA CCTACAAGAA GAAGATACAC GTATGCAACA GGCAGATAAA 
GACTGGAAGA AAGTAGCAAA ACAGGAAGAT CTGAAAGAAA TGGGTGATGT GTCCTCAGGG 
ATGAGTAGCT CCATTATGCA GCTTTACCTC AAACAAGTTC TTGAGGCTTT CTTTCACACT 
CAGTCAAGTG TGCGCCACTT TGCCCTAAAT GTCATTGCAT TGACTCTCAA CCAGGGGCTC 
ATTCATCCTG TTCAGTGTGT GCCATATTTA ATTGCTATGG GCACAGACCC TGAACCTGCT 
ATGCGGAATA AAGCCGACCA GCAGCTTGTG GAAATAGACA AAAAATATGC TGGATTCATT 
CACATGAAAG CTGTGGCTGG TATGAAGATG TCTTACCAAG TTCAGCAAGC CATCAACACA 
TCCCCAAAAG ATGCCGTGAG GGGCTTCAGA CAAGATGAAT CCTCCAGTGC TTTATGTTCA 
CATCTTTACT CCATGATCCG TGGAAATCGC CAACACAGAC GAGCCTTTCT AATTGCTTTA 
CTCAATCTCT TTGATGACAC AGCAAAAACA GAAGTGAATA TGCTCTTGTA TATAGCAGAC 
AATCTAGCCT GTTTCCCTTA CCAAACACAG GAAGAACCAT TGTTTATAAT GCATCATATA 
GATATTACAC TCTCAGTTTC TGGCAGTAAC CTTCTGCAGT CATTTAAGGA GTCTATGGTG 
AAGGACAAGC GGAAAGAGAA GAAACCCTCA CCAAATCGGG AAAGTGCATC TGGAAGTGAT 
AGTGAAGGAG AGGTTTCCAA GCCTCGAAAG TCACGTAAAC GAGTTGACTC TGATTCAGAT 
TCAGATGATG AAGATGACAT AAATGCTGTG ATGAAATGTT TACCAGATAA TTCAAGTCCT 
TTAATTGAGT TTGCAAATGT GTCCCAGGGT ATTTTATTAC TTCTGATGTT AAAACAACAT 
TTAAAGAACC TATGTGGATT CTCTGATAGT AAAATCCAGA AGTATTCTCC ATCTGAATCT 
GCAAAAGTAT ATGATAAAGC GATAAACCGG AAAACAGGAA TCCATTTTCA TCCTAAACAA 
ACCTTAGACT TCCTGCGGAG TGATATGGCT AATTCCAAAA TTACAGAAGA TGTGAAAAGG 
AGTATAGTTA AACAATATCT AGATTTCAAG CTCCTCATGG AACATCTGGA TCCTGATGAA 
GAAGAAGAAG AAGGGGAGGT GTCAGCTAGC ACAAATGCTC GGAACAAAGC AATTACCTCA 
CTGCTTGGAG GAGGCAGCCC TAAAAATAAT GCAGCTGAGA CAGAAGATGA TGAAAGTGAT 
GGGGAGGATA GAGGAGGAGG CACTTCAGGG GTGAGGCGGA GGAGGAGTCA ACGTATTTCG 
CAGCGTATTA CTCATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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NIPBL cDNA ORF clone, Monodelphis domestica(gray short-tailed opossum)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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