LOC101486624 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101486624
Entrez Gene ID	101486624
Full Name	FRAS1-related extracellular matrix protein 1
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024800597.1	XP_024656365.1	LOW QUALITY PROTEIN: FRAS1-related extracellular matrix protein 1
XM_004556999.1	XP_004557056.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_012920781.1	XP_012776235.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_012920782.1	XP_012776236.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_004557000.1	XP_004557057.1	FRAS1-related extracellular matrix protein 1 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC101486624 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101486624 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa02957	XM_012920781.1 Latest version!	Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa02957	XM_012920782.1 Latest version!	Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa02957	XM_004556999.1 Latest version!	Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa02958	XM_004557000.1 Latest version!	Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OMa357637	XM_024800597.1 Latest version!	Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101486624 ( XM_004556999.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004557056.1 Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa02957
Clone ID Related Accession (Same CDS sequence)	XM_004556999.1 , XM_012920781.1 , XM_012920782.1
Accession Version	XM_004556999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444631.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCG TCCTCGTGTC CCTGCTGCTG CTCGCGGTGA CGGGCGTTTG CCCTCGGACT 
CTGGTTGTGG TGAACTCTGC CGTGGAGGTC AGCAGAGGGC GCTCGGTTTT CATCACGAGG 
GCGGAGCTTC AGATCAGCGT GGACCCGAGC GCCGACTGTA AGGTGGAGGT GGTGATGAAC 
GAGCCCGTGA CTCAGAGGGT CGGGAGGCTG AGCCCACAGG TGTTTGACTG CAGCTTCCTG 
GAGGACGAGG TGAAGTATGT CCACGACGGC AGCCCTCTGC TGGACGAGGA CGTGGTGATG 
CTCAGAGTCT ACAGGTTCAC GTCCTCGGAC ACGCAGGTGG AGACGGTGAT CCTGAGGGTC 
CGGGTGGTGG ACTCTGGTGC GGGCGTGGTG GCACTCGGCA GCGCTCCGCT GGTCGTTCCC 
CAGTTCTACG GTTTATCCAA CGCCATCAAC GGCTCTGTGC TGAATATCAG GGCCGCGCCT 
GACCTTGTCT GCACCGTCAG GCTGTTGACC GCAGACACCA GCGTCCCCGC TGCGGGTCGG 
CTGGTGAGGG AGGAAGACTC CACGCAGAGG AAGGGTCGAC AGGCGACGCT CTGCCCAGGA 
AACAGTCCGT GTCTTCACGA CACCACCGAG GTCCACTTCC TCAAAACCAG CTGCCATGAA 
TTCCTGAGCT CTGCCCTCAA GTACCAGCAC CTCAGCCCGC CGTCCCCGGA GATCGACTAC 
GTCCCCGTCA GGGTGGAGCT GAGAGAGCAG GCGAGCAGAG CACTGCTGGA GTCACAGGCC 
GTGTGGCTGC CGGTTTTGAT CCACGGGGCC ATGCAGAACC AGCCCCCGCA GGCTACCTTC 
ATGGCCTCTT TCATCCTGGA GGTGGACCAG TTCATCCTCA CCCCGCTCAC CACTGCCACG 
CTGGACGCCA AGGACCACGA GACTCCGCAG GAGAAGCTGG TCTTCAATGT GACCGTCCCA 
CCCGCCGAGG GCTACATCAC ACACCTGGAC GACCACACCA AGCCCATCCG GTCCTTCACA 
TGGCTGGACC TTCACGAGAT GAAAGTGGCC TACCAGCCAT CCAACAGCAG CCGGGGGCGC 
CGCAGGAACT ATGAGGTGGA GTTTCAGGTG ATAGACGGCT CCTATCTGAC CAGCCCACCC 
ATCGTGGTCC ACGTCTCCAT CAGGACATCG GAAACGAATG CTCCGAGAGT CTCCTGGAAC 
GTGGGTCTAG ACCTGTTGGA GGGTCAGTCC CGACCAATCA CCTGGGAGCA GCTGCAGATA 
GTCGACAGCG ACAACATTGA CGCCGTTTAC CTGGTGGCCG TGGACGGACC CCTGCACGGG 
CGGCTCAGTG TCAGAGGCGG GAAGGCGTTT ATGTTCCGCG TGCGGGACCT GAAGGAGGGC 
ACAGTCGTCT ACCACCACTC CGACAGCGAC TCCACCCGCG ACCACGTCGT CTTCCGCATC 
AGCGACGGTC GTCACAGCGT CCGCCACAAG TTCCCCATCA ACATCCTCCC GAAGGACGAC 
TCTCCGCCCT TCCTCATCAA CAACGTGGCG GTGGAGGTGC AGGAGGGTGG GGCCGTGCGG 
CTCGAGGAGT TCATGCTGCT GGCCTCCGAC CTCGACTCCA GCGACGACTA CATCCTTTAT 
CAGATAGTCT CCGCCCCCCG GGCCGGGCAC CTGGTCAGGA GGACATCCGC CCATGAGACA 
GGGTCACCGG TGGCCAGCTT CCTGCAGAGG GACCTGATCC AGGGTCAGAT CTTCTACCAG 
CACTCGGGAG ATGAGCAGTT TGAGGATGCC TTCGACTTCA CGCTGTCTGA CAGCCACCGG 
CCGCCCAACC TGTCGCAGAC ATACACGGTG GTGGTGCACG TGTTCCCGGT GGAGGACCAG 
GTGCCAGTCG AAGTTCCCGG CAGCGTCCGC TCGCTGACAG TGAAGGAGAC GGAGGTGGTT 
TACATCACCC AGGCTCACCT TCACTTCACA GACCGAGAGC ACCCAGATGC TGACCTCACC 
TATGTCATTA CACAGCCCTG CTTCAGCCCG CTACATCCTG GCCTGATGGA TGCAGGACGT 
CTGTTTTACA CCGACAGCAC CAACGCCATG AAGAAGGACC ACACAGCGCC CGTGCTGAAG 
TCCTTCACTC AGCATGCTGT GAACCACATG AAGGTGGCCT TCATGCCGCC GGTCGAGGAC 
ATTGGGCCCG AGTCGCTCTT CGTCCAGTTC GTCTTCTCAG TCAGCGATCA CCACGGCGAC 
ACCGTCTCCG GCCTCATCTT CAACATCACG GTTACCCCTG TCGATGACCA GGCGCCAGAG 
GCTTTCACCA ACCTGCTTCG GGTGGAGGAG GGCGGCGCGG CCTTCGTGAC GGAGGAGCAC 
CTCTTGGTCC GGGACCGGGA CAGTCGAGAG GAGGCGCTGA GGGTGGAGAT TCGGAGCACG 
GCTCGTCACG GCCGGCTGGA GCTGTGGGGC CAAGCGCTGC AGCAGGGAGG CGGGTTTACC 
CTGCAGGAAC TGAGAGGGCT GGGCATCAGG TACATCCACG ATGACTCTGA GACCACTGAG 
GATGTCGTTG GTCTCAAGGT GTCGGACGGT CTGAACTCTG TGGACGTCGT CCTGCACGTT 
CAGGTGCTGC CGATGAACGA CGAGCCTCCT CAGCTGGGCG CCCGTCTGCA AGGGGGGCTG 
AGCTGCGAGG AAGGGGGGCG TGTCCAGGTG ATGGTGGACT ACCTGTCGGC CACCGACCGG 
GACAGCGACG ATTCCGCGCT GACCTACATG CTCGCCCGGA GACCGAGCAG GGGAGAGCTG 
CAGCGAGCCG GCGTGACTGT GGACAAGTTC TCCCAGCAGG ACGTCCTGCA GGGTCACATC 
TACTATGCCC ACACAGGCGG GGAGATCGGA CCTGAGCCGG TGTTCGACAC GGTCACCCTC 
ATCATCTCCG ACAGTGAGGC CAGAGGGCCG GACAGCTGCT GCCACGGGGA GGCCCCGCCC 
CCTCCTGTTC CTCTCCATGG TACACTTCCT GTTTACGATC TGAACATCAC AGTTCTTCCT 
GTCAACGATA AAGTCCCTGC TGTCACTCTG GGCGAGTCCA TGCTGGTGGT GGATGAAGGC 
TCTTCTATCT GTCTGTGCGG GGGGGTCCTC GGAGCGTCAG ACCCAGACAG TCCCCCTGAG 
CAGCTCGTCT TTCACCTGGA GACTCCTCCC CTTCACGGCT TCCTGGAGAA CACGCAGCCG 
ATGCCCGGCT CCGAAAAGAG CAACGCGGGA GTGCGAGTCG AATCCTTCAC TCTCAAACAC 
CTGACCTCTG GCTTCATCAA CTACGTGCAA TCGGAGCACA AAGGGGTGGA GCCAACTGTC 
GACCAGCTGT CCATCAGTGT GAGCGACGGG CTACATCGCT CCGCCCCCGT CTCCTTCTAC 
ATCATTATCA AACCAACCAA TGACGAAACA CCATCGCTGC TGCTCGCCAA CTTCACCGTA 
AAAGAGGGCG GGATGAGGGA GCTAATTCCA TCCATACTGG ATGGTTTTGA CTTGGACGCC 
CCACCTGAGC TGCTCACCTT CACTGTGGTG CAGCCGCCGG CTCACGGCAG CCTGATTAAC 
AGCATCCACG GCACGGAGGC GGGAGCTGAC CTCCTGCACA GGAGCCTCTC TGTCACCTCT 
TTCACTCTGC AGGAGCTCCG ACAAGGCATG AGGATCGTCT ACATGCACGA TGACACAGAA 
ACCCTGAAGG ACGCCGTGGC TCTGCGGCTG ACAGATGGTG TCCATGCAGT CGAGGGAACA 
GTTCAGGTCA CCGTGCTGCT GGTCAACGAC GAGAAACCAC GACTGCTCAA GAATGCTGGA 
CTGCAGGTGG ACTCAGGTGA GCTCAGGGTG ATCTCCAGTG TGGTTCTGGA GGCTGAGGAC 
CTGGACACGC CCCCAGAACA GGTGTACTAC TTCCTTAATG TTGCTCCGCG GTTTGGAAAA 
CTGCAGCTGA AGACGACGTC GGGGTGGAAC GAGCTGACGG CGGGTCAGAA CTTCACACAG 
GGCGACGTGG AGATGAACCG ACTGTGGTAC CAACACACCG CCGCCACCAA CGCAGCCGGC 
TTTAAAGGTC ACGACAGCTT CCGCTTCACC CTCAGCGACC TGGACAACGA GTCTCCGGCT 
CAGAGCTTCT TCATCTCTGT GCACGTGATG CAGAAAGGGG ACATCGTGGT GCTGAGCAGG 
CCAGTGCACC TGAGGGAGGG GCAGCGTGTC GTCCTGAACA CTGACATCCT GCTGGCATCA 
GACTCGACTG GCCGGCCCAA GGAGCTGGTC TACACGGTGT CTGACCCGCC GCGTCACGGC 
CTCGTCCACG CCGTCCACCG GCCTGGAGTC CCGCTACTCA GCTTCACCCA GCTGGACGTG 
GCTGCGCACC GAGTCTGCTA CACCCACGAC AACAGCCATG ATGCCGAGGC TGACTCCTTC 
AGTTTTGTTG TTACAAACGG TGACTCGTCT CGCAGCTCCA CCCTCCACTT CACCATCGAG 
CATGGTGACC GCATCCCGCC CACCCTCAGC CGCAACGTTG GCCTCCGGCT GCAGGATGGC 
TCCACAGTGA CCATCACTTC GGACCAGCTG CAGCTCACCG ACCCCGACAC TACCACCGCC 
AACCTGGGAT ACGTCATCAC GCAGCTGCCG CAGCACGGGA AGCTGCTGCT GAGAGGCACG 
CCGCTACCCT CGCCGCCCCG GTTCTCCCAG ACGAACGTGG ACGACCTCCA CCTGACTTAC 
CAACACGACC CGAGCAGCCC TGCGAAGATC GACGGGTTCT ACTTTATGCC GAGCGACGGA 
CACAACAGGG GCTACCTGGA GTTTGGGCAG CTGAGGGAGG AGCCTGCTGT GTTTGACATA 
CAGGTGGATT GGGTCGACCG GACGCCTCCC ACTCTGACCA CCAGGGGGAG TCCCAGCACC 
GTGGTGGATC TGGGTGGCGG CCGTTACGGA ATCTTCATCA CCAGCAGAGA GCTGCAGGCC 
TCAGACCCTG ACAGCCCCAC AGAGGAGCTC GAGTTCTCCA TCAGCAGACC TCCACTCTTA 
GGATACCTGG AGAACGCCGC CACAGGGTCG TACATCAAAG GTCGTTTCAC GCAGCGTGAC 
GTGGATCGGC GAGCCGTGAT GTTCGTCCTC CCCGCTGACG TGGAAGTGAC GTCTGACAGC 
TTCCTGTTTG TCCTCAGAGA TCCGGCAGGA AACAGCGCGT TGCCTCAGAC GCTGGAGCTG 
TCCTGGTCTC GAGTGGAGCT GTCGGCGACC TGCTACAGAA CCTGTGAGAC GGCCGGGATA 
CTTCAGATCC GCGTGCAGCG CAACGGGAGG AGCGCTGACC CTGCCTACGT CGCCATCCAG 
GTGGACGAAG GATCAGCCAA AGCTGGCAGA GATTTCACTC ACAGCACTGC GAGTCTCATC 
CAGTTCGATC CAGGTGTGAA GGTGAAAACC TGGAACATTT ACCTGATTGA CGACGGCCTG 
GAAGAAAACC ACGAGAGCTT CACGGTCACG CTGAGAAACC CCCAGAACGC CGTCCTGGGA 
CCAAGGAGCT CGGCCACCGT GGAGATCATT GACCCCCACC GAGGAAGCTG TGACCCAGAC 
GACCTGTGGG TAGAAGATGA TCAGCGCCCC CTTCCTCCTC CTCCTACTCC TCCCCCTCTC 
CCTCCTCAGC TCCCTGATCC TCTGCAGCAA GAGGAGGAAG AGGAGGTTCC GGTCATCAAT 
ATAGAGGCGG AGCTGCTGTG GGAGAACCAG CCTCACCCGC CCCGAGGAGA CGTCCCGAAT 
CGACATCACC TGGACTACAA AGAGGTGGAG CCACGAGACC CAGAGCCCCA CAGCAATGGC 
TACACCCCCC ACAGCAGCCA CCTAAACATT CAGCAGCACA CTGGAAGCAG TACTGGGAAC 
ACTGGGAGCA CGGTCCGTCT GTCTGCACAG AGGAGGCCTG AGGACGAGCA GGAGAAGGTT 
TGGACGTTCC ACAGTCTGAC TCCTCTCCGG CTGGAGGAGC TGCAACCGGG CGATGTCGAG 
TGGAGCTGGT CAGCTCACGG TCGTCCCCTG CAGGAACTCC AAGTTGGAGA TTCTCCTCAG 
ATGGCTCATC AAGCTCAGAA ACACCAACCC GAGCTCCGCC AGCACAAGGT GGGGAAGTCT 
GTGAGCAGCT CATGTCCTGA TGGCTGGACG CACCACAGGA GACGATGTTA CATCCTCAGC 
CCGTCCGTGG CGAGCTGGGC CAGCGCCGAC AGAACCTGCT CGCTGCTGTT TAACAGCAGC 
CTGACCGACG TGCACTCCAG GAGAGATGTG AGCTGGTTGT GGAGGTTTGC AGGGAAGAAG 
CCATTTTGGA TCGGTCTGTC TGGAGGTCCA GGACGCTCAT CGTGGGCTGA CAGTCGCTCG 
CTGTCTTCCT CCAGACTGAG GGGGGCGCCA GTGAGCCCCG GTGAGCCCGA GAACGACTCC 
AACTGCGTGC TGGTTCAAAA CCCGAGAAGC TGGATCTCCA CGAGCTGCTC TGCAGAAACT 
CAGCACTCAT TCATCTGTTC ATCGCCAGCT CAAACTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004557056.1
CDS	303..6764
Translation	MAAVLVSLLLLAVTGVCPRTLVVVNSAVEVSRGRSVFITRAELQISVDPSADCKVEVVMNEPVTQRVGRLSPQVFDCSFLEDEVKYVHDGSPLLDEDVVMLRVYRFTSSDTQVETVILRVRVVDSGAGVVALGSAPLVVPQFYGLSNAINGSVLNIRAAPDLVCTVRLLTADTSVPAAGRLVREEDSTQRKGRQATLCPGNSPCLHDTTEVHFLKTSCHEFLSSALKYQHLSPPSPEIDYVPVRVELREQASRALLESQAVWLPVLIHGAMQNQPPQATFMASFILEVDQFILTPLTTATLDAKDHETPQEKLVFNVTVPPAEGYITHLDDHTKPIRSFTWLDLHEMKVAYQPSNSSRGRRRNYEVEFQVIDGSYLTSPPIVVHVSIRTSETNAPRVSWNVGLDLLEGQSRPITWEQLQIVDSDNIDAVYLVAVDGPLHGRLSVRGGKAFMFRVRDLKEGTVVYHHSDSDSTRDHVVFRISDGRHSVRHKFPINILPKDDSPPFLINNVAVEVQEGGAVRLEEFMLLASDLDSSDDYILYQIVSAPRAGHLVRRTSAHETGSPVASFLQRDLIQGQIFYQHSGDEQFEDAFDFTLSDSHRPPNLSQTYTVVVHVFPVEDQVPVEVPGSVRSLTVKETEVVYITQAHLHFTDREHPDADLTYVITQPCFSPLHPGLMDAGRLFYTDSTNAMKKDHTAPVLKSFTQHAVNHMKVAFMPPVEDIGPESLFVQFVFSVSDHHGDTVSGLIFNITVTPVDDQAPEAFTNLLRVEEGGAAFVTEEHLLVRDRDSREEALRVEIRSTARHGRLELWGQALQQGGGFTLQELRGLGIRYIHDDSETTEDVVGLKVSDGLNSVDVVLHVQVLPMNDEPPQLGARLQGGLSCEEGGRVQVMVDYLSATDRDSDDSALTYMLARRPSRGELQRAGVTVDKFSQQDVLQGHIYYAHTGGEIGPEPVFDTVTLIISDSEARGPDSCCHGEAPPPPVPLHGTLPVYDLNITVLPVNDKVPAVTLGESMLVVDEGSSICLCGGVLGASDPDSPPEQLVFHLETPPLHGFLENTQPMPGSEKSNAGVRVESFTLKHLTSGFINYVQSEHKGVEPTVDQLSISVSDGLHRSAPVSFYIIIKPTNDETPSLLLANFTVKEGGMRELIPSILDGFDLDAPPELLTFTVVQPPAHGSLINSIHGTEAGADLLHRSLSVTSFTLQELRQGMRIVYMHDDTETLKDAVALRLTDGVHAVEGTVQVTVLLVNDEKPRLLKNAGLQVDSGELRVISSVVLEAEDLDTPPEQVYYFLNVAPRFGKLQLKTTSGWNELTAGQNFTQGDVEMNRLWYQHTAATNAAGFKGHDSFRFTLSDLDNESPAQSFFISVHVMQKGDIVVLSRPVHLREGQRVVLNTDILLASDSTGRPKELVYTVSDPPRHGLVHAVHRPGVPLLSFTQLDVAAHRVCYTHDNSHDAEADSFSFVVTNGDSSRSSTLHFTIEHGDRIPPTLSRNVGLRLQDGSTVTITSDQLQLTDPDTTTANLGYVITQLPQHGKLLLRGTPLPSPPRFSQTNVDDLHLTYQHDPSSPAKIDGFYFMPSDGHNRGYLEFGQLREEPAVFDIQVDWVDRTPPTLTTRGSPSTVVDLGGGRYGIFITSRELQASDPDSPTEELEFSISRPPLLGYLENAATGSYIKGRFTQRDVDRRAVMFVLPADVEVTSDSFLFVLRDPAGNSALPQTLELSWSRVELSATCYRTCETAGILQIRVQRNGRSADPAYVAIQVDEGSAKAGRDFTHSTASLIQFDPGVKVKTWNIYLIDDGLEENHESFTVTLRNPQNAVLGPRSSATVEIIDPHRGSCDPDDLWVEDDQRPLPPPPTPPPLPPQLPDPLQQEEEEEVPVINIEAELLWENQPHPPRGDVPNRHHLDYKEVEPRDPEPHSNGYTPHSSHLNIQQHTGSSTGNTGSTVRLSAQRRPEDEQEKVWTFHSLTPLRLEELQPGDVEWSWSAHGRPLQELQVGDSPQMAHQAQKHQPELRQHKVGKSVSSSCPDGWTHHRRRCYILSPSVASWASADRTCSLLFNSSLTDVHSRRDVSWLWRFAGKKPFWIGLSGGPGRSSWADSRSLSSSRLRGAPVSPGEPENDSNCVLVQNPRSWISTSCSAETQHSFICSSPAQTH

Target ORF information:

RefSeq Version	XM_004556999.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004556999.1

ORF Insert Sequence:

ATGGCTGCCG TCCTCGTGTC CCTGCTGCTG CTCGCGGTGA CGGGCGTTTG CCCTCGGACT 
CTGGTTGTGG TGAACTCTGC CGTGGAGGTC AGCAGAGGGC GCTCGGTTTT CATCACGAGG 
GCGGAGCTTC AGATCAGCGT GGACCCGAGC GCCGACTGTA AGGTGGAGGT GGTGATGAAC 
GAGCCCGTGA CTCAGAGGGT CGGGAGGCTG AGCCCACAGG TGTTTGACTG CAGCTTCCTG 
GAGGACGAGG TGAAGTATGT CCACGACGGC AGCCCTCTGC TGGACGAGGA CGTGGTGATG 
CTCAGAGTCT ACAGGTTCAC GTCCTCGGAC ACGCAGGTGG AGACGGTGAT CCTGAGGGTC 
CGGGTGGTGG ACTCTGGTGC GGGCGTGGTG GCACTCGGCA GCGCTCCGCT GGTCGTTCCC 
CAGTTCTACG GTTTATCCAA CGCCATCAAC GGCTCTGTGC TGAATATCAG GGCCGCGCCT 
GACCTTGTCT GCACCGTCAG GCTGTTGACC GCAGACACCA GCGTCCCCGC TGCGGGTCGG 
CTGGTGAGGG AGGAAGACTC CACGCAGAGG AAGGGTCGAC AGGCGACGCT CTGCCCAGGA 
AACAGTCCGT GTCTTCACGA CACCACCGAG GTCCACTTCC TCAAAACCAG CTGCCATGAA 
TTCCTGAGCT CTGCCCTCAA GTACCAGCAC CTCAGCCCGC CGTCCCCGGA GATCGACTAC 
GTCCCCGTCA GGGTGGAGCT GAGAGAGCAG GCGAGCAGAG CACTGCTGGA GTCACAGGCC 
GTGTGGCTGC CGGTTTTGAT CCACGGGGCC ATGCAGAACC AGCCCCCGCA GGCTACCTTC 
ATGGCCTCTT TCATCCTGGA GGTGGACCAG TTCATCCTCA CCCCGCTCAC CACTGCCACG 
CTGGACGCCA AGGACCACGA GACTCCGCAG GAGAAGCTGG TCTTCAATGT GACCGTCCCA 
CCCGCCGAGG GCTACATCAC ACACCTGGAC GACCACACCA AGCCCATCCG GTCCTTCACA 
TGGCTGGACC TTCACGAGAT GAAAGTGGCC TACCAGCCAT CCAACAGCAG CCGGGGGCGC 
CGCAGGAACT ATGAGGTGGA GTTTCAGGTG ATAGACGGCT CCTATCTGAC CAGCCCACCC 
ATCGTGGTCC ACGTCTCCAT CAGGACATCG GAAACGAATG CTCCGAGAGT CTCCTGGAAC 
GTGGGTCTAG ACCTGTTGGA GGGTCAGTCC CGACCAATCA CCTGGGAGCA GCTGCAGATA 
GTCGACAGCG ACAACATTGA CGCCGTTTAC CTGGTGGCCG TGGACGGACC CCTGCACGGG 
CGGCTCAGTG TCAGAGGCGG GAAGGCGTTT ATGTTCCGCG TGCGGGACCT GAAGGAGGGC 
ACAGTCGTCT ACCACCACTC CGACAGCGAC TCCACCCGCG ACCACGTCGT CTTCCGCATC 
AGCGACGGTC GTCACAGCGT CCGCCACAAG TTCCCCATCA ACATCCTCCC GAAGGACGAC 
TCTCCGCCCT TCCTCATCAA CAACGTGGCG GTGGAGGTGC AGGAGGGTGG GGCCGTGCGG 
CTCGAGGAGT TCATGCTGCT GGCCTCCGAC CTCGACTCCA GCGACGACTA CATCCTTTAT 
CAGATAGTCT CCGCCCCCCG GGCCGGGCAC CTGGTCAGGA GGACATCCGC CCATGAGACA 
GGGTCACCGG TGGCCAGCTT CCTGCAGAGG GACCTGATCC AGGGTCAGAT CTTCTACCAG 
CACTCGGGAG ATGAGCAGTT TGAGGATGCC TTCGACTTCA CGCTGTCTGA CAGCCACCGG 
CCGCCCAACC TGTCGCAGAC ATACACGGTG GTGGTGCACG TGTTCCCGGT GGAGGACCAG 
GTGCCAGTCG AAGTTCCCGG CAGCGTCCGC TCGCTGACAG TGAAGGAGAC GGAGGTGGTT 
TACATCACCC AGGCTCACCT TCACTTCACA GACCGAGAGC ACCCAGATGC TGACCTCACC 
TATGTCATTA CACAGCCCTG CTTCAGCCCG CTACATCCTG GCCTGATGGA TGCAGGACGT 
CTGTTTTACA CCGACAGCAC CAACGCCATG AAGAAGGACC ACACAGCGCC CGTGCTGAAG 
TCCTTCACTC AGCATGCTGT GAACCACATG AAGGTGGCCT TCATGCCGCC GGTCGAGGAC 
ATTGGGCCCG AGTCGCTCTT CGTCCAGTTC GTCTTCTCAG TCAGCGATCA CCACGGCGAC 
ACCGTCTCCG GCCTCATCTT CAACATCACG GTTACCCCTG TCGATGACCA GGCGCCAGAG 
GCTTTCACCA ACCTGCTTCG GGTGGAGGAG GGCGGCGCGG CCTTCGTGAC GGAGGAGCAC 
CTCTTGGTCC GGGACCGGGA CAGTCGAGAG GAGGCGCTGA GGGTGGAGAT TCGGAGCACG 
GCTCGTCACG GCCGGCTGGA GCTGTGGGGC CAAGCGCTGC AGCAGGGAGG CGGGTTTACC 
CTGCAGGAAC TGAGAGGGCT GGGCATCAGG TACATCCACG ATGACTCTGA GACCACTGAG 
GATGTCGTTG GTCTCAAGGT GTCGGACGGT CTGAACTCTG TGGACGTCGT CCTGCACGTT 
CAGGTGCTGC CGATGAACGA CGAGCCTCCT CAGCTGGGCG CCCGTCTGCA AGGGGGGCTG 
AGCTGCGAGG AAGGGGGGCG TGTCCAGGTG ATGGTGGACT ACCTGTCGGC CACCGACCGG 
GACAGCGACG ATTCCGCGCT GACCTACATG CTCGCCCGGA GACCGAGCAG GGGAGAGCTG 
CAGCGAGCCG GCGTGACTGT GGACAAGTTC TCCCAGCAGG ACGTCCTGCA GGGTCACATC 
TACTATGCCC ACACAGGCGG GGAGATCGGA CCTGAGCCGG TGTTCGACAC GGTCACCCTC 
ATCATCTCCG ACAGTGAGGC CAGAGGGCCG GACAGCTGCT GCCACGGGGA GGCCCCGCCC 
CCTCCTGTTC CTCTCCATGG TACACTTCCT GTTTACGATC TGAACATCAC AGTTCTTCCT 
GTCAACGATA AAGTCCCTGC TGTCACTCTG GGCGAGTCCA TGCTGGTGGT GGATGAAGGC 
TCTTCTATCT GTCTGTGCGG GGGGGTCCTC GGAGCGTCAG ACCCAGACAG TCCCCCTGAG 
CAGCTCGTCT TTCACCTGGA GACTCCTCCC CTTCACGGCT TCCTGGAGAA CACGCAGCCG 
ATGCCCGGCT CCGAAAAGAG CAACGCGGGA GTGCGAGTCG AATCCTTCAC TCTCAAACAC 
CTGACCTCTG GCTTCATCAA CTACGTGCAA TCGGAGCACA AAGGGGTGGA GCCAACTGTC 
GACCAGCTGT CCATCAGTGT GAGCGACGGG CTACATCGCT CCGCCCCCGT CTCCTTCTAC 
ATCATTATCA AACCAACCAA TGACGAAACA CCATCGCTGC TGCTCGCCAA CTTCACCGTA 
AAAGAGGGCG GGATGAGGGA GCTAATTCCA TCCATACTGG ATGGTTTTGA CTTGGACGCC 
CCACCTGAGC TGCTCACCTT CACTGTGGTG CAGCCGCCGG CTCACGGCAG CCTGATTAAC 
AGCATCCACG GCACGGAGGC GGGAGCTGAC CTCCTGCACA GGAGCCTCTC TGTCACCTCT 
TTCACTCTGC AGGAGCTCCG ACAAGGCATG AGGATCGTCT ACATGCACGA TGACACAGAA 
ACCCTGAAGG ACGCCGTGGC TCTGCGGCTG ACAGATGGTG TCCATGCAGT CGAGGGAACA 
GTTCAGGTCA CCGTGCTGCT GGTCAACGAC GAGAAACCAC GACTGCTCAA GAATGCTGGA 
CTGCAGGTGG ACTCAGGTGA GCTCAGGGTG ATCTCCAGTG TGGTTCTGGA GGCTGAGGAC 
CTGGACACGC CCCCAGAACA GGTGTACTAC TTCCTTAATG TTGCTCCGCG GTTTGGAAAA 
CTGCAGCTGA AGACGACGTC GGGGTGGAAC GAGCTGACGG CGGGTCAGAA CTTCACACAG 
GGCGACGTGG AGATGAACCG ACTGTGGTAC CAACACACCG CCGCCACCAA CGCAGCCGGC 
TTTAAAGGTC ACGACAGCTT CCGCTTCACC CTCAGCGACC TGGACAACGA GTCTCCGGCT 
CAGAGCTTCT TCATCTCTGT GCACGTGATG CAGAAAGGGG ACATCGTGGT GCTGAGCAGG 
CCAGTGCACC TGAGGGAGGG GCAGCGTGTC GTCCTGAACA CTGACATCCT GCTGGCATCA 
GACTCGACTG GCCGGCCCAA GGAGCTGGTC TACACGGTGT CTGACCCGCC GCGTCACGGC 
CTCGTCCACG CCGTCCACCG GCCTGGAGTC CCGCTACTCA GCTTCACCCA GCTGGACGTG 
GCTGCGCACC GAGTCTGCTA CACCCACGAC AACAGCCATG ATGCCGAGGC TGACTCCTTC 
AGTTTTGTTG TTACAAACGG TGACTCGTCT CGCAGCTCCA CCCTCCACTT CACCATCGAG 
CATGGTGACC GCATCCCGCC CACCCTCAGC CGCAACGTTG GCCTCCGGCT GCAGGATGGC 
TCCACAGTGA CCATCACTTC GGACCAGCTG CAGCTCACCG ACCCCGACAC TACCACCGCC 
AACCTGGGAT ACGTCATCAC GCAGCTGCCG CAGCACGGGA AGCTGCTGCT GAGAGGCACG 
CCGCTACCCT CGCCGCCCCG GTTCTCCCAG ACGAACGTGG ACGACCTCCA CCTGACTTAC 
CAACACGACC CGAGCAGCCC TGCGAAGATC GACGGGTTCT ACTTTATGCC GAGCGACGGA 
CACAACAGGG GCTACCTGGA GTTTGGGCAG CTGAGGGAGG AGCCTGCTGT GTTTGACATA 
CAGGTGGATT GGGTCGACCG GACGCCTCCC ACTCTGACCA CCAGGGGGAG TCCCAGCACC 
GTGGTGGATC TGGGTGGCGG CCGTTACGGA ATCTTCATCA CCAGCAGAGA GCTGCAGGCC 
TCAGACCCTG ACAGCCCCAC AGAGGAGCTC GAGTTCTCCA TCAGCAGACC TCCACTCTTA 
GGATACCTGG AGAACGCCGC CACAGGGTCG TACATCAAAG GTCGTTTCAC GCAGCGTGAC 
GTGGATCGGC GAGCCGTGAT GTTCGTCCTC CCCGCTGACG TGGAAGTGAC GTCTGACAGC 
TTCCTGTTTG TCCTCAGAGA TCCGGCAGGA AACAGCGCGT TGCCTCAGAC GCTGGAGCTG 
TCCTGGTCTC GAGTGGAGCT GTCGGCGACC TGCTACAGAA CCTGTGAGAC GGCCGGGATA 
CTTCAGATCC GCGTGCAGCG CAACGGGAGG AGCGCTGACC CTGCCTACGT CGCCATCCAG 
GTGGACGAAG GATCAGCCAA AGCTGGCAGA GATTTCACTC ACAGCACTGC GAGTCTCATC 
CAGTTCGATC CAGGTGTGAA GGTGAAAACC TGGAACATTT ACCTGATTGA CGACGGCCTG 
GAAGAAAACC ACGAGAGCTT CACGGTCACG CTGAGAAACC CCCAGAACGC CGTCCTGGGA 
CCAAGGAGCT CGGCCACCGT GGAGATCATT GACCCCCACC GAGGAAGCTG TGACCCAGAC 
GACCTGTGGG TAGAAGATGA TCAGCGCCCC CTTCCTCCTC CTCCTACTCC TCCCCCTCTC 
CCTCCTCAGC TCCCTGATCC TCTGCAGCAA GAGGAGGAAG AGGAGGTTCC GGTCATCAAT 
ATAGAGGCGG AGCTGCTGTG GGAGAACCAG CCTCACCCGC CCCGAGGAGA CGTCCCGAAT 
CGACATCACC TGGACTACAA AGAGGTGGAG CCACGAGACC CAGAGCCCCA CAGCAATGGC 
TACACCCCCC ACAGCAGCCA CCTAAACATT CAGCAGCACA CTGGAAGCAG TACTGGGAAC 
ACTGGGAGCA CGGTCCGTCT GTCTGCACAG AGGAGGCCTG AGGACGAGCA GGAGAAGGTT 
TGGACGTTCC ACAGTCTGAC TCCTCTCCGG CTGGAGGAGC TGCAACCGGG CGATGTCGAG 
TGGAGCTGGT CAGCTCACGG TCGTCCCCTG CAGGAACTCC AAGTTGGAGA TTCTCCTCAG 
ATGGCTCATC AAGCTCAGAA ACACCAACCC GAGCTCCGCC AGCACAAGGT GGGGAAGTCT 
GTGAGCAGCT CATGTCCTGA TGGCTGGACG CACCACAGGA GACGATGTTA CATCCTCAGC 
CCGTCCGTGG CGAGCTGGGC CAGCGCCGAC AGAACCTGCT CGCTGCTGTT TAACAGCAGC 
CTGACCGACG TGCACTCCAG GAGAGATGTG AGCTGGTTGT GGAGGTTTGC AGGGAAGAAG 
CCATTTTGGA TCGGTCTGTC TGGAGGTCCA GGACGCTCAT CGTGGGCTGA CAGTCGCTCG 
CTGTCTTCCT CCAGACTGAG GGGGGCGCCA GTGAGCCCCG GTGAGCCCGA GAACGACTCC 
AACTGCGTGC TGGTTCAAAA CCCGAGAAGC TGGATCTCCA CGAGCTGCTC TGCAGAAACT 
CAGCACTCAT TCATCTGTTC ATCGCCAGCT CAAACTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101486624 ( XM_012920781.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012776235.1 Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), transcript variant X2, mRNA.

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CloneID	OMa02957
Clone ID Related Accession (Same CDS sequence)	XM_004556999.1 , XM_012920781.1 , XM_012920782.1
Accession Version	XM_012920781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444631.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCG TCCTCGTGTC CCTGCTGCTG CTCGCGGTGA CGGGCGTTTG CCCTCGGACT 
CTGGTTGTGG TGAACTCTGC CGTGGAGGTC AGCAGAGGGC GCTCGGTTTT CATCACGAGG 
GCGGAGCTTC AGATCAGCGT GGACCCGAGC GCCGACTGTA AGGTGGAGGT GGTGATGAAC 
GAGCCCGTGA CTCAGAGGGT CGGGAGGCTG AGCCCACAGG TGTTTGACTG CAGCTTCCTG 
GAGGACGAGG TGAAGTATGT CCACGACGGC AGCCCTCTGC TGGACGAGGA CGTGGTGATG 
CTCAGAGTCT ACAGGTTCAC GTCCTCGGAC ACGCAGGTGG AGACGGTGAT CCTGAGGGTC 
CGGGTGGTGG ACTCTGGTGC GGGCGTGGTG GCACTCGGCA GCGCTCCGCT GGTCGTTCCC 
CAGTTCTACG GTTTATCCAA CGCCATCAAC GGCTCTGTGC TGAATATCAG GGCCGCGCCT 
GACCTTGTCT GCACCGTCAG GCTGTTGACC GCAGACACCA GCGTCCCCGC TGCGGGTCGG 
CTGGTGAGGG AGGAAGACTC CACGCAGAGG AAGGGTCGAC AGGCGACGCT CTGCCCAGGA 
AACAGTCCGT GTCTTCACGA CACCACCGAG GTCCACTTCC TCAAAACCAG CTGCCATGAA 
TTCCTGAGCT CTGCCCTCAA GTACCAGCAC CTCAGCCCGC CGTCCCCGGA GATCGACTAC 
GTCCCCGTCA GGGTGGAGCT GAGAGAGCAG GCGAGCAGAG CACTGCTGGA GTCACAGGCC 
GTGTGGCTGC CGGTTTTGAT CCACGGGGCC ATGCAGAACC AGCCCCCGCA GGCTACCTTC 
ATGGCCTCTT TCATCCTGGA GGTGGACCAG TTCATCCTCA CCCCGCTCAC CACTGCCACG 
CTGGACGCCA AGGACCACGA GACTCCGCAG GAGAAGCTGG TCTTCAATGT GACCGTCCCA 
CCCGCCGAGG GCTACATCAC ACACCTGGAC GACCACACCA AGCCCATCCG GTCCTTCACA 
TGGCTGGACC TTCACGAGAT GAAAGTGGCC TACCAGCCAT CCAACAGCAG CCGGGGGCGC 
CGCAGGAACT ATGAGGTGGA GTTTCAGGTG ATAGACGGCT CCTATCTGAC CAGCCCACCC 
ATCGTGGTCC ACGTCTCCAT CAGGACATCG GAAACGAATG CTCCGAGAGT CTCCTGGAAC 
GTGGGTCTAG ACCTGTTGGA GGGTCAGTCC CGACCAATCA CCTGGGAGCA GCTGCAGATA 
GTCGACAGCG ACAACATTGA CGCCGTTTAC CTGGTGGCCG TGGACGGACC CCTGCACGGG 
CGGCTCAGTG TCAGAGGCGG GAAGGCGTTT ATGTTCCGCG TGCGGGACCT GAAGGAGGGC 
ACAGTCGTCT ACCACCACTC CGACAGCGAC TCCACCCGCG ACCACGTCGT CTTCCGCATC 
AGCGACGGTC GTCACAGCGT CCGCCACAAG TTCCCCATCA ACATCCTCCC GAAGGACGAC 
TCTCCGCCCT TCCTCATCAA CAACGTGGCG GTGGAGGTGC AGGAGGGTGG GGCCGTGCGG 
CTCGAGGAGT TCATGCTGCT GGCCTCCGAC CTCGACTCCA GCGACGACTA CATCCTTTAT 
CAGATAGTCT CCGCCCCCCG GGCCGGGCAC CTGGTCAGGA GGACATCCGC CCATGAGACA 
GGGTCACCGG TGGCCAGCTT CCTGCAGAGG GACCTGATCC AGGGTCAGAT CTTCTACCAG 
CACTCGGGAG ATGAGCAGTT TGAGGATGCC TTCGACTTCA CGCTGTCTGA CAGCCACCGG 
CCGCCCAACC TGTCGCAGAC ATACACGGTG GTGGTGCACG TGTTCCCGGT GGAGGACCAG 
GTGCCAGTCG AAGTTCCCGG CAGCGTCCGC TCGCTGACAG TGAAGGAGAC GGAGGTGGTT 
TACATCACCC AGGCTCACCT TCACTTCACA GACCGAGAGC ACCCAGATGC TGACCTCACC 
TATGTCATTA CACAGCCCTG CTTCAGCCCG CTACATCCTG GCCTGATGGA TGCAGGACGT 
CTGTTTTACA CCGACAGCAC CAACGCCATG AAGAAGGACC ACACAGCGCC CGTGCTGAAG 
TCCTTCACTC AGCATGCTGT GAACCACATG AAGGTGGCCT TCATGCCGCC GGTCGAGGAC 
ATTGGGCCCG AGTCGCTCTT CGTCCAGTTC GTCTTCTCAG TCAGCGATCA CCACGGCGAC 
ACCGTCTCCG GCCTCATCTT CAACATCACG GTTACCCCTG TCGATGACCA GGCGCCAGAG 
GCTTTCACCA ACCTGCTTCG GGTGGAGGAG GGCGGCGCGG CCTTCGTGAC GGAGGAGCAC 
CTCTTGGTCC GGGACCGGGA CAGTCGAGAG GAGGCGCTGA GGGTGGAGAT TCGGAGCACG 
GCTCGTCACG GCCGGCTGGA GCTGTGGGGC CAAGCGCTGC AGCAGGGAGG CGGGTTTACC 
CTGCAGGAAC TGAGAGGGCT GGGCATCAGG TACATCCACG ATGACTCTGA GACCACTGAG 
GATGTCGTTG GTCTCAAGGT GTCGGACGGT CTGAACTCTG TGGACGTCGT CCTGCACGTT 
CAGGTGCTGC CGATGAACGA CGAGCCTCCT CAGCTGGGCG CCCGTCTGCA AGGGGGGCTG 
AGCTGCGAGG AAGGGGGGCG TGTCCAGGTG ATGGTGGACT ACCTGTCGGC CACCGACCGG 
GACAGCGACG ATTCCGCGCT GACCTACATG CTCGCCCGGA GACCGAGCAG GGGAGAGCTG 
CAGCGAGCCG GCGTGACTGT GGACAAGTTC TCCCAGCAGG ACGTCCTGCA GGGTCACATC 
TACTATGCCC ACACAGGCGG GGAGATCGGA CCTGAGCCGG TGTTCGACAC GGTCACCCTC 
ATCATCTCCG ACAGTGAGGC CAGAGGGCCG GACAGCTGCT GCCACGGGGA GGCCCCGCCC 
CCTCCTGTTC CTCTCCATGG TACACTTCCT GTTTACGATC TGAACATCAC AGTTCTTCCT 
GTCAACGATA AAGTCCCTGC TGTCACTCTG GGCGAGTCCA TGCTGGTGGT GGATGAAGGC 
TCTTCTATCT GTCTGTGCGG GGGGGTCCTC GGAGCGTCAG ACCCAGACAG TCCCCCTGAG 
CAGCTCGTCT TTCACCTGGA GACTCCTCCC CTTCACGGCT TCCTGGAGAA CACGCAGCCG 
ATGCCCGGCT CCGAAAAGAG CAACGCGGGA GTGCGAGTCG AATCCTTCAC TCTCAAACAC 
CTGACCTCTG GCTTCATCAA CTACGTGCAA TCGGAGCACA AAGGGGTGGA GCCAACTGTC 
GACCAGCTGT CCATCAGTGT GAGCGACGGG CTACATCGCT CCGCCCCCGT CTCCTTCTAC 
ATCATTATCA AACCAACCAA TGACGAAACA CCATCGCTGC TGCTCGCCAA CTTCACCGTA 
AAAGAGGGCG GGATGAGGGA GCTAATTCCA TCCATACTGG ATGGTTTTGA CTTGGACGCC 
CCACCTGAGC TGCTCACCTT CACTGTGGTG CAGCCGCCGG CTCACGGCAG CCTGATTAAC 
AGCATCCACG GCACGGAGGC GGGAGCTGAC CTCCTGCACA GGAGCCTCTC TGTCACCTCT 
TTCACTCTGC AGGAGCTCCG ACAAGGCATG AGGATCGTCT ACATGCACGA TGACACAGAA 
ACCCTGAAGG ACGCCGTGGC TCTGCGGCTG ACAGATGGTG TCCATGCAGT CGAGGGAACA 
GTTCAGGTCA CCGTGCTGCT GGTCAACGAC GAGAAACCAC GACTGCTCAA GAATGCTGGA 
CTGCAGGTGG ACTCAGGTGA GCTCAGGGTG ATCTCCAGTG TGGTTCTGGA GGCTGAGGAC 
CTGGACACGC CCCCAGAACA GGTGTACTAC TTCCTTAATG TTGCTCCGCG GTTTGGAAAA 
CTGCAGCTGA AGACGACGTC GGGGTGGAAC GAGCTGACGG CGGGTCAGAA CTTCACACAG 
GGCGACGTGG AGATGAACCG ACTGTGGTAC CAACACACCG CCGCCACCAA CGCAGCCGGC 
TTTAAAGGTC ACGACAGCTT CCGCTTCACC CTCAGCGACC TGGACAACGA GTCTCCGGCT 
CAGAGCTTCT TCATCTCTGT GCACGTGATG CAGAAAGGGG ACATCGTGGT GCTGAGCAGG 
CCAGTGCACC TGAGGGAGGG GCAGCGTGTC GTCCTGAACA CTGACATCCT GCTGGCATCA 
GACTCGACTG GCCGGCCCAA GGAGCTGGTC TACACGGTGT CTGACCCGCC GCGTCACGGC 
CTCGTCCACG CCGTCCACCG GCCTGGAGTC CCGCTACTCA GCTTCACCCA GCTGGACGTG 
GCTGCGCACC GAGTCTGCTA CACCCACGAC AACAGCCATG ATGCCGAGGC TGACTCCTTC 
AGTTTTGTTG TTACAAACGG TGACTCGTCT CGCAGCTCCA CCCTCCACTT CACCATCGAG 
CATGGTGACC GCATCCCGCC CACCCTCAGC CGCAACGTTG GCCTCCGGCT GCAGGATGGC 
TCCACAGTGA CCATCACTTC GGACCAGCTG CAGCTCACCG ACCCCGACAC TACCACCGCC 
AACCTGGGAT ACGTCATCAC GCAGCTGCCG CAGCACGGGA AGCTGCTGCT GAGAGGCACG 
CCGCTACCCT CGCCGCCCCG GTTCTCCCAG ACGAACGTGG ACGACCTCCA CCTGACTTAC 
CAACACGACC CGAGCAGCCC TGCGAAGATC GACGGGTTCT ACTTTATGCC GAGCGACGGA 
CACAACAGGG GCTACCTGGA GTTTGGGCAG CTGAGGGAGG AGCCTGCTGT GTTTGACATA 
CAGGTGGATT GGGTCGACCG GACGCCTCCC ACTCTGACCA CCAGGGGGAG TCCCAGCACC 
GTGGTGGATC TGGGTGGCGG CCGTTACGGA ATCTTCATCA CCAGCAGAGA GCTGCAGGCC 
TCAGACCCTG ACAGCCCCAC AGAGGAGCTC GAGTTCTCCA TCAGCAGACC TCCACTCTTA 
GGATACCTGG AGAACGCCGC CACAGGGTCG TACATCAAAG GTCGTTTCAC GCAGCGTGAC 
GTGGATCGGC GAGCCGTGAT GTTCGTCCTC CCCGCTGACG TGGAAGTGAC GTCTGACAGC 
TTCCTGTTTG TCCTCAGAGA TCCGGCAGGA AACAGCGCGT TGCCTCAGAC GCTGGAGCTG 
TCCTGGTCTC GAGTGGAGCT GTCGGCGACC TGCTACAGAA CCTGTGAGAC GGCCGGGATA 
CTTCAGATCC GCGTGCAGCG CAACGGGAGG AGCGCTGACC CTGCCTACGT CGCCATCCAG 
GTGGACGAAG GATCAGCCAA AGCTGGCAGA GATTTCACTC ACAGCACTGC GAGTCTCATC 
CAGTTCGATC CAGGTGTGAA GGTGAAAACC TGGAACATTT ACCTGATTGA CGACGGCCTG 
GAAGAAAACC ACGAGAGCTT CACGGTCACG CTGAGAAACC CCCAGAACGC CGTCCTGGGA 
CCAAGGAGCT CGGCCACCGT GGAGATCATT GACCCCCACC GAGGAAGCTG TGACCCAGAC 
GACCTGTGGG TAGAAGATGA TCAGCGCCCC CTTCCTCCTC CTCCTACTCC TCCCCCTCTC 
CCTCCTCAGC TCCCTGATCC TCTGCAGCAA GAGGAGGAAG AGGAGGTTCC GGTCATCAAT 
ATAGAGGCGG AGCTGCTGTG GGAGAACCAG CCTCACCCGC CCCGAGGAGA CGTCCCGAAT 
CGACATCACC TGGACTACAA AGAGGTGGAG CCACGAGACC CAGAGCCCCA CAGCAATGGC 
TACACCCCCC ACAGCAGCCA CCTAAACATT CAGCAGCACA CTGGAAGCAG TACTGGGAAC 
ACTGGGAGCA CGGTCCGTCT GTCTGCACAG AGGAGGCCTG AGGACGAGCA GGAGAAGGTT 
TGGACGTTCC ACAGTCTGAC TCCTCTCCGG CTGGAGGAGC TGCAACCGGG CGATGTCGAG 
TGGAGCTGGT CAGCTCACGG TCGTCCCCTG CAGGAACTCC AAGTTGGAGA TTCTCCTCAG 
ATGGCTCATC AAGCTCAGAA ACACCAACCC GAGCTCCGCC AGCACAAGGT GGGGAAGTCT 
GTGAGCAGCT CATGTCCTGA TGGCTGGACG CACCACAGGA GACGATGTTA CATCCTCAGC 
CCGTCCGTGG CGAGCTGGGC CAGCGCCGAC AGAACCTGCT CGCTGCTGTT TAACAGCAGC 
CTGACCGACG TGCACTCCAG GAGAGATGTG AGCTGGTTGT GGAGGTTTGC AGGGAAGAAG 
CCATTTTGGA TCGGTCTGTC TGGAGGTCCA GGACGCTCAT CGTGGGCTGA CAGTCGCTCG 
CTGTCTTCCT CCAGACTGAG GGGGGCGCCA GTGAGCCCCG GTGAGCCCGA GAACGACTCC 
AACTGCGTGC TGGTTCAAAA CCCGAGAAGC TGGATCTCCA CGAGCTGCTC TGCAGAAACT 
CAGCACTCAT TCATCTGTTC ATCGCCAGCT CAAACTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012776235.1
CDS	539..7000
Translation	MAAVLVSLLLLAVTGVCPRTLVVVNSAVEVSRGRSVFITRAELQISVDPSADCKVEVVMNEPVTQRVGRLSPQVFDCSFLEDEVKYVHDGSPLLDEDVVMLRVYRFTSSDTQVETVILRVRVVDSGAGVVALGSAPLVVPQFYGLSNAINGSVLNIRAAPDLVCTVRLLTADTSVPAAGRLVREEDSTQRKGRQATLCPGNSPCLHDTTEVHFLKTSCHEFLSSALKYQHLSPPSPEIDYVPVRVELREQASRALLESQAVWLPVLIHGAMQNQPPQATFMASFILEVDQFILTPLTTATLDAKDHETPQEKLVFNVTVPPAEGYITHLDDHTKPIRSFTWLDLHEMKVAYQPSNSSRGRRRNYEVEFQVIDGSYLTSPPIVVHVSIRTSETNAPRVSWNVGLDLLEGQSRPITWEQLQIVDSDNIDAVYLVAVDGPLHGRLSVRGGKAFMFRVRDLKEGTVVYHHSDSDSTRDHVVFRISDGRHSVRHKFPINILPKDDSPPFLINNVAVEVQEGGAVRLEEFMLLASDLDSSDDYILYQIVSAPRAGHLVRRTSAHETGSPVASFLQRDLIQGQIFYQHSGDEQFEDAFDFTLSDSHRPPNLSQTYTVVVHVFPVEDQVPVEVPGSVRSLTVKETEVVYITQAHLHFTDREHPDADLTYVITQPCFSPLHPGLMDAGRLFYTDSTNAMKKDHTAPVLKSFTQHAVNHMKVAFMPPVEDIGPESLFVQFVFSVSDHHGDTVSGLIFNITVTPVDDQAPEAFTNLLRVEEGGAAFVTEEHLLVRDRDSREEALRVEIRSTARHGRLELWGQALQQGGGFTLQELRGLGIRYIHDDSETTEDVVGLKVSDGLNSVDVVLHVQVLPMNDEPPQLGARLQGGLSCEEGGRVQVMVDYLSATDRDSDDSALTYMLARRPSRGELQRAGVTVDKFSQQDVLQGHIYYAHTGGEIGPEPVFDTVTLIISDSEARGPDSCCHGEAPPPPVPLHGTLPVYDLNITVLPVNDKVPAVTLGESMLVVDEGSSICLCGGVLGASDPDSPPEQLVFHLETPPLHGFLENTQPMPGSEKSNAGVRVESFTLKHLTSGFINYVQSEHKGVEPTVDQLSISVSDGLHRSAPVSFYIIIKPTNDETPSLLLANFTVKEGGMRELIPSILDGFDLDAPPELLTFTVVQPPAHGSLINSIHGTEAGADLLHRSLSVTSFTLQELRQGMRIVYMHDDTETLKDAVALRLTDGVHAVEGTVQVTVLLVNDEKPRLLKNAGLQVDSGELRVISSVVLEAEDLDTPPEQVYYFLNVAPRFGKLQLKTTSGWNELTAGQNFTQGDVEMNRLWYQHTAATNAAGFKGHDSFRFTLSDLDNESPAQSFFISVHVMQKGDIVVLSRPVHLREGQRVVLNTDILLASDSTGRPKELVYTVSDPPRHGLVHAVHRPGVPLLSFTQLDVAAHRVCYTHDNSHDAEADSFSFVVTNGDSSRSSTLHFTIEHGDRIPPTLSRNVGLRLQDGSTVTITSDQLQLTDPDTTTANLGYVITQLPQHGKLLLRGTPLPSPPRFSQTNVDDLHLTYQHDPSSPAKIDGFYFMPSDGHNRGYLEFGQLREEPAVFDIQVDWVDRTPPTLTTRGSPSTVVDLGGGRYGIFITSRELQASDPDSPTEELEFSISRPPLLGYLENAATGSYIKGRFTQRDVDRRAVMFVLPADVEVTSDSFLFVLRDPAGNSALPQTLELSWSRVELSATCYRTCETAGILQIRVQRNGRSADPAYVAIQVDEGSAKAGRDFTHSTASLIQFDPGVKVKTWNIYLIDDGLEENHESFTVTLRNPQNAVLGPRSSATVEIIDPHRGSCDPDDLWVEDDQRPLPPPPTPPPLPPQLPDPLQQEEEEEVPVINIEAELLWENQPHPPRGDVPNRHHLDYKEVEPRDPEPHSNGYTPHSSHLNIQQHTGSSTGNTGSTVRLSAQRRPEDEQEKVWTFHSLTPLRLEELQPGDVEWSWSAHGRPLQELQVGDSPQMAHQAQKHQPELRQHKVGKSVSSSCPDGWTHHRRRCYILSPSVASWASADRTCSLLFNSSLTDVHSRRDVSWLWRFAGKKPFWIGLSGGPGRSSWADSRSLSSSRLRGAPVSPGEPENDSNCVLVQNPRSWISTSCSAETQHSFICSSPAQTH

Target ORF information:

RefSeq Version	XM_012920781.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012920781.1

ORF Insert Sequence:

ATGGCTGCCG TCCTCGTGTC CCTGCTGCTG CTCGCGGTGA CGGGCGTTTG CCCTCGGACT 
CTGGTTGTGG TGAACTCTGC CGTGGAGGTC AGCAGAGGGC GCTCGGTTTT CATCACGAGG 
GCGGAGCTTC AGATCAGCGT GGACCCGAGC GCCGACTGTA AGGTGGAGGT GGTGATGAAC 
GAGCCCGTGA CTCAGAGGGT CGGGAGGCTG AGCCCACAGG TGTTTGACTG CAGCTTCCTG 
GAGGACGAGG TGAAGTATGT CCACGACGGC AGCCCTCTGC TGGACGAGGA CGTGGTGATG 
CTCAGAGTCT ACAGGTTCAC GTCCTCGGAC ACGCAGGTGG AGACGGTGAT CCTGAGGGTC 
CGGGTGGTGG ACTCTGGTGC GGGCGTGGTG GCACTCGGCA GCGCTCCGCT GGTCGTTCCC 
CAGTTCTACG GTTTATCCAA CGCCATCAAC GGCTCTGTGC TGAATATCAG GGCCGCGCCT 
GACCTTGTCT GCACCGTCAG GCTGTTGACC GCAGACACCA GCGTCCCCGC TGCGGGTCGG 
CTGGTGAGGG AGGAAGACTC CACGCAGAGG AAGGGTCGAC AGGCGACGCT CTGCCCAGGA 
AACAGTCCGT GTCTTCACGA CACCACCGAG GTCCACTTCC TCAAAACCAG CTGCCATGAA 
TTCCTGAGCT CTGCCCTCAA GTACCAGCAC CTCAGCCCGC CGTCCCCGGA GATCGACTAC 
GTCCCCGTCA GGGTGGAGCT GAGAGAGCAG GCGAGCAGAG CACTGCTGGA GTCACAGGCC 
GTGTGGCTGC CGGTTTTGAT CCACGGGGCC ATGCAGAACC AGCCCCCGCA GGCTACCTTC 
ATGGCCTCTT TCATCCTGGA GGTGGACCAG TTCATCCTCA CCCCGCTCAC CACTGCCACG 
CTGGACGCCA AGGACCACGA GACTCCGCAG GAGAAGCTGG TCTTCAATGT GACCGTCCCA 
CCCGCCGAGG GCTACATCAC ACACCTGGAC GACCACACCA AGCCCATCCG GTCCTTCACA 
TGGCTGGACC TTCACGAGAT GAAAGTGGCC TACCAGCCAT CCAACAGCAG CCGGGGGCGC 
CGCAGGAACT ATGAGGTGGA GTTTCAGGTG ATAGACGGCT CCTATCTGAC CAGCCCACCC 
ATCGTGGTCC ACGTCTCCAT CAGGACATCG GAAACGAATG CTCCGAGAGT CTCCTGGAAC 
GTGGGTCTAG ACCTGTTGGA GGGTCAGTCC CGACCAATCA CCTGGGAGCA GCTGCAGATA 
GTCGACAGCG ACAACATTGA CGCCGTTTAC CTGGTGGCCG TGGACGGACC CCTGCACGGG 
CGGCTCAGTG TCAGAGGCGG GAAGGCGTTT ATGTTCCGCG TGCGGGACCT GAAGGAGGGC 
ACAGTCGTCT ACCACCACTC CGACAGCGAC TCCACCCGCG ACCACGTCGT CTTCCGCATC 
AGCGACGGTC GTCACAGCGT CCGCCACAAG TTCCCCATCA ACATCCTCCC GAAGGACGAC 
TCTCCGCCCT TCCTCATCAA CAACGTGGCG GTGGAGGTGC AGGAGGGTGG GGCCGTGCGG 
CTCGAGGAGT TCATGCTGCT GGCCTCCGAC CTCGACTCCA GCGACGACTA CATCCTTTAT 
CAGATAGTCT CCGCCCCCCG GGCCGGGCAC CTGGTCAGGA GGACATCCGC CCATGAGACA 
GGGTCACCGG TGGCCAGCTT CCTGCAGAGG GACCTGATCC AGGGTCAGAT CTTCTACCAG 
CACTCGGGAG ATGAGCAGTT TGAGGATGCC TTCGACTTCA CGCTGTCTGA CAGCCACCGG 
CCGCCCAACC TGTCGCAGAC ATACACGGTG GTGGTGCACG TGTTCCCGGT GGAGGACCAG 
GTGCCAGTCG AAGTTCCCGG CAGCGTCCGC TCGCTGACAG TGAAGGAGAC GGAGGTGGTT 
TACATCACCC AGGCTCACCT TCACTTCACA GACCGAGAGC ACCCAGATGC TGACCTCACC 
TATGTCATTA CACAGCCCTG CTTCAGCCCG CTACATCCTG GCCTGATGGA TGCAGGACGT 
CTGTTTTACA CCGACAGCAC CAACGCCATG AAGAAGGACC ACACAGCGCC CGTGCTGAAG 
TCCTTCACTC AGCATGCTGT GAACCACATG AAGGTGGCCT TCATGCCGCC GGTCGAGGAC 
ATTGGGCCCG AGTCGCTCTT CGTCCAGTTC GTCTTCTCAG TCAGCGATCA CCACGGCGAC 
ACCGTCTCCG GCCTCATCTT CAACATCACG GTTACCCCTG TCGATGACCA GGCGCCAGAG 
GCTTTCACCA ACCTGCTTCG GGTGGAGGAG GGCGGCGCGG CCTTCGTGAC GGAGGAGCAC 
CTCTTGGTCC GGGACCGGGA CAGTCGAGAG GAGGCGCTGA GGGTGGAGAT TCGGAGCACG 
GCTCGTCACG GCCGGCTGGA GCTGTGGGGC CAAGCGCTGC AGCAGGGAGG CGGGTTTACC 
CTGCAGGAAC TGAGAGGGCT GGGCATCAGG TACATCCACG ATGACTCTGA GACCACTGAG 
GATGTCGTTG GTCTCAAGGT GTCGGACGGT CTGAACTCTG TGGACGTCGT CCTGCACGTT 
CAGGTGCTGC CGATGAACGA CGAGCCTCCT CAGCTGGGCG CCCGTCTGCA AGGGGGGCTG 
AGCTGCGAGG AAGGGGGGCG TGTCCAGGTG ATGGTGGACT ACCTGTCGGC CACCGACCGG 
GACAGCGACG ATTCCGCGCT GACCTACATG CTCGCCCGGA GACCGAGCAG GGGAGAGCTG 
CAGCGAGCCG GCGTGACTGT GGACAAGTTC TCCCAGCAGG ACGTCCTGCA GGGTCACATC 
TACTATGCCC ACACAGGCGG GGAGATCGGA CCTGAGCCGG TGTTCGACAC GGTCACCCTC 
ATCATCTCCG ACAGTGAGGC CAGAGGGCCG GACAGCTGCT GCCACGGGGA GGCCCCGCCC 
CCTCCTGTTC CTCTCCATGG TACACTTCCT GTTTACGATC TGAACATCAC AGTTCTTCCT 
GTCAACGATA AAGTCCCTGC TGTCACTCTG GGCGAGTCCA TGCTGGTGGT GGATGAAGGC 
TCTTCTATCT GTCTGTGCGG GGGGGTCCTC GGAGCGTCAG ACCCAGACAG TCCCCCTGAG 
CAGCTCGTCT TTCACCTGGA GACTCCTCCC CTTCACGGCT TCCTGGAGAA CACGCAGCCG 
ATGCCCGGCT CCGAAAAGAG CAACGCGGGA GTGCGAGTCG AATCCTTCAC TCTCAAACAC 
CTGACCTCTG GCTTCATCAA CTACGTGCAA TCGGAGCACA AAGGGGTGGA GCCAACTGTC 
GACCAGCTGT CCATCAGTGT GAGCGACGGG CTACATCGCT CCGCCCCCGT CTCCTTCTAC 
ATCATTATCA AACCAACCAA TGACGAAACA CCATCGCTGC TGCTCGCCAA CTTCACCGTA 
AAAGAGGGCG GGATGAGGGA GCTAATTCCA TCCATACTGG ATGGTTTTGA CTTGGACGCC 
CCACCTGAGC TGCTCACCTT CACTGTGGTG CAGCCGCCGG CTCACGGCAG CCTGATTAAC 
AGCATCCACG GCACGGAGGC GGGAGCTGAC CTCCTGCACA GGAGCCTCTC TGTCACCTCT 
TTCACTCTGC AGGAGCTCCG ACAAGGCATG AGGATCGTCT ACATGCACGA TGACACAGAA 
ACCCTGAAGG ACGCCGTGGC TCTGCGGCTG ACAGATGGTG TCCATGCAGT CGAGGGAACA 
GTTCAGGTCA CCGTGCTGCT GGTCAACGAC GAGAAACCAC GACTGCTCAA GAATGCTGGA 
CTGCAGGTGG ACTCAGGTGA GCTCAGGGTG ATCTCCAGTG TGGTTCTGGA GGCTGAGGAC 
CTGGACACGC CCCCAGAACA GGTGTACTAC TTCCTTAATG TTGCTCCGCG GTTTGGAAAA 
CTGCAGCTGA AGACGACGTC GGGGTGGAAC GAGCTGACGG CGGGTCAGAA CTTCACACAG 
GGCGACGTGG AGATGAACCG ACTGTGGTAC CAACACACCG CCGCCACCAA CGCAGCCGGC 
TTTAAAGGTC ACGACAGCTT CCGCTTCACC CTCAGCGACC TGGACAACGA GTCTCCGGCT 
CAGAGCTTCT TCATCTCTGT GCACGTGATG CAGAAAGGGG ACATCGTGGT GCTGAGCAGG 
CCAGTGCACC TGAGGGAGGG GCAGCGTGTC GTCCTGAACA CTGACATCCT GCTGGCATCA 
GACTCGACTG GCCGGCCCAA GGAGCTGGTC TACACGGTGT CTGACCCGCC GCGTCACGGC 
CTCGTCCACG CCGTCCACCG GCCTGGAGTC CCGCTACTCA GCTTCACCCA GCTGGACGTG 
GCTGCGCACC GAGTCTGCTA CACCCACGAC AACAGCCATG ATGCCGAGGC TGACTCCTTC 
AGTTTTGTTG TTACAAACGG TGACTCGTCT CGCAGCTCCA CCCTCCACTT CACCATCGAG 
CATGGTGACC GCATCCCGCC CACCCTCAGC CGCAACGTTG GCCTCCGGCT GCAGGATGGC 
TCCACAGTGA CCATCACTTC GGACCAGCTG CAGCTCACCG ACCCCGACAC TACCACCGCC 
AACCTGGGAT ACGTCATCAC GCAGCTGCCG CAGCACGGGA AGCTGCTGCT GAGAGGCACG 
CCGCTACCCT CGCCGCCCCG GTTCTCCCAG ACGAACGTGG ACGACCTCCA CCTGACTTAC 
CAACACGACC CGAGCAGCCC TGCGAAGATC GACGGGTTCT ACTTTATGCC GAGCGACGGA 
CACAACAGGG GCTACCTGGA GTTTGGGCAG CTGAGGGAGG AGCCTGCTGT GTTTGACATA 
CAGGTGGATT GGGTCGACCG GACGCCTCCC ACTCTGACCA CCAGGGGGAG TCCCAGCACC 
GTGGTGGATC TGGGTGGCGG CCGTTACGGA ATCTTCATCA CCAGCAGAGA GCTGCAGGCC 
TCAGACCCTG ACAGCCCCAC AGAGGAGCTC GAGTTCTCCA TCAGCAGACC TCCACTCTTA 
GGATACCTGG AGAACGCCGC CACAGGGTCG TACATCAAAG GTCGTTTCAC GCAGCGTGAC 
GTGGATCGGC GAGCCGTGAT GTTCGTCCTC CCCGCTGACG TGGAAGTGAC GTCTGACAGC 
TTCCTGTTTG TCCTCAGAGA TCCGGCAGGA AACAGCGCGT TGCCTCAGAC GCTGGAGCTG 
TCCTGGTCTC GAGTGGAGCT GTCGGCGACC TGCTACAGAA CCTGTGAGAC GGCCGGGATA 
CTTCAGATCC GCGTGCAGCG CAACGGGAGG AGCGCTGACC CTGCCTACGT CGCCATCCAG 
GTGGACGAAG GATCAGCCAA AGCTGGCAGA GATTTCACTC ACAGCACTGC GAGTCTCATC 
CAGTTCGATC CAGGTGTGAA GGTGAAAACC TGGAACATTT ACCTGATTGA CGACGGCCTG 
GAAGAAAACC ACGAGAGCTT CACGGTCACG CTGAGAAACC CCCAGAACGC CGTCCTGGGA 
CCAAGGAGCT CGGCCACCGT GGAGATCATT GACCCCCACC GAGGAAGCTG TGACCCAGAC 
GACCTGTGGG TAGAAGATGA TCAGCGCCCC CTTCCTCCTC CTCCTACTCC TCCCCCTCTC 
CCTCCTCAGC TCCCTGATCC TCTGCAGCAA GAGGAGGAAG AGGAGGTTCC GGTCATCAAT 
ATAGAGGCGG AGCTGCTGTG GGAGAACCAG CCTCACCCGC CCCGAGGAGA CGTCCCGAAT 
CGACATCACC TGGACTACAA AGAGGTGGAG CCACGAGACC CAGAGCCCCA CAGCAATGGC 
TACACCCCCC ACAGCAGCCA CCTAAACATT CAGCAGCACA CTGGAAGCAG TACTGGGAAC 
ACTGGGAGCA CGGTCCGTCT GTCTGCACAG AGGAGGCCTG AGGACGAGCA GGAGAAGGTT 
TGGACGTTCC ACAGTCTGAC TCCTCTCCGG CTGGAGGAGC TGCAACCGGG CGATGTCGAG 
TGGAGCTGGT CAGCTCACGG TCGTCCCCTG CAGGAACTCC AAGTTGGAGA TTCTCCTCAG 
ATGGCTCATC AAGCTCAGAA ACACCAACCC GAGCTCCGCC AGCACAAGGT GGGGAAGTCT 
GTGAGCAGCT CATGTCCTGA TGGCTGGACG CACCACAGGA GACGATGTTA CATCCTCAGC 
CCGTCCGTGG CGAGCTGGGC CAGCGCCGAC AGAACCTGCT CGCTGCTGTT TAACAGCAGC 
CTGACCGACG TGCACTCCAG GAGAGATGTG AGCTGGTTGT GGAGGTTTGC AGGGAAGAAG 
CCATTTTGGA TCGGTCTGTC TGGAGGTCCA GGACGCTCAT CGTGGGCTGA CAGTCGCTCG 
CTGTCTTCCT CCAGACTGAG GGGGGCGCCA GTGAGCCCCG GTGAGCCCGA GAACGACTCC 
AACTGCGTGC TGGTTCAAAA CCCGAGAAGC TGGATCTCCA CGAGCTGCTC TGCAGAAACT 
CAGCACTCAT TCATCTGTTC ATCGCCAGCT CAAACTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101486624 ( XM_012920782.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012776236.1 Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), transcript variant X3, mRNA.

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CloneID	OMa02957
Clone ID Related Accession (Same CDS sequence)	XM_004556999.1 , XM_012920781.1 , XM_012920782.1
Accession Version	XM_012920782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444631.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCG TCCTCGTGTC CCTGCTGCTG CTCGCGGTGA CGGGCGTTTG CCCTCGGACT 
CTGGTTGTGG TGAACTCTGC CGTGGAGGTC AGCAGAGGGC GCTCGGTTTT CATCACGAGG 
GCGGAGCTTC AGATCAGCGT GGACCCGAGC GCCGACTGTA AGGTGGAGGT GGTGATGAAC 
GAGCCCGTGA CTCAGAGGGT CGGGAGGCTG AGCCCACAGG TGTTTGACTG CAGCTTCCTG 
GAGGACGAGG TGAAGTATGT CCACGACGGC AGCCCTCTGC TGGACGAGGA CGTGGTGATG 
CTCAGAGTCT ACAGGTTCAC GTCCTCGGAC ACGCAGGTGG AGACGGTGAT CCTGAGGGTC 
CGGGTGGTGG ACTCTGGTGC GGGCGTGGTG GCACTCGGCA GCGCTCCGCT GGTCGTTCCC 
CAGTTCTACG GTTTATCCAA CGCCATCAAC GGCTCTGTGC TGAATATCAG GGCCGCGCCT 
GACCTTGTCT GCACCGTCAG GCTGTTGACC GCAGACACCA GCGTCCCCGC TGCGGGTCGG 
CTGGTGAGGG AGGAAGACTC CACGCAGAGG AAGGGTCGAC AGGCGACGCT CTGCCCAGGA 
AACAGTCCGT GTCTTCACGA CACCACCGAG GTCCACTTCC TCAAAACCAG CTGCCATGAA 
TTCCTGAGCT CTGCCCTCAA GTACCAGCAC CTCAGCCCGC CGTCCCCGGA GATCGACTAC 
GTCCCCGTCA GGGTGGAGCT GAGAGAGCAG GCGAGCAGAG CACTGCTGGA GTCACAGGCC 
GTGTGGCTGC CGGTTTTGAT CCACGGGGCC ATGCAGAACC AGCCCCCGCA GGCTACCTTC 
ATGGCCTCTT TCATCCTGGA GGTGGACCAG TTCATCCTCA CCCCGCTCAC CACTGCCACG 
CTGGACGCCA AGGACCACGA GACTCCGCAG GAGAAGCTGG TCTTCAATGT GACCGTCCCA 
CCCGCCGAGG GCTACATCAC ACACCTGGAC GACCACACCA AGCCCATCCG GTCCTTCACA 
TGGCTGGACC TTCACGAGAT GAAAGTGGCC TACCAGCCAT CCAACAGCAG CCGGGGGCGC 
CGCAGGAACT ATGAGGTGGA GTTTCAGGTG ATAGACGGCT CCTATCTGAC CAGCCCACCC 
ATCGTGGTCC ACGTCTCCAT CAGGACATCG GAAACGAATG CTCCGAGAGT CTCCTGGAAC 
GTGGGTCTAG ACCTGTTGGA GGGTCAGTCC CGACCAATCA CCTGGGAGCA GCTGCAGATA 
GTCGACAGCG ACAACATTGA CGCCGTTTAC CTGGTGGCCG TGGACGGACC CCTGCACGGG 
CGGCTCAGTG TCAGAGGCGG GAAGGCGTTT ATGTTCCGCG TGCGGGACCT GAAGGAGGGC 
ACAGTCGTCT ACCACCACTC CGACAGCGAC TCCACCCGCG ACCACGTCGT CTTCCGCATC 
AGCGACGGTC GTCACAGCGT CCGCCACAAG TTCCCCATCA ACATCCTCCC GAAGGACGAC 
TCTCCGCCCT TCCTCATCAA CAACGTGGCG GTGGAGGTGC AGGAGGGTGG GGCCGTGCGG 
CTCGAGGAGT TCATGCTGCT GGCCTCCGAC CTCGACTCCA GCGACGACTA CATCCTTTAT 
CAGATAGTCT CCGCCCCCCG GGCCGGGCAC CTGGTCAGGA GGACATCCGC CCATGAGACA 
GGGTCACCGG TGGCCAGCTT CCTGCAGAGG GACCTGATCC AGGGTCAGAT CTTCTACCAG 
CACTCGGGAG ATGAGCAGTT TGAGGATGCC TTCGACTTCA CGCTGTCTGA CAGCCACCGG 
CCGCCCAACC TGTCGCAGAC ATACACGGTG GTGGTGCACG TGTTCCCGGT GGAGGACCAG 
GTGCCAGTCG AAGTTCCCGG CAGCGTCCGC TCGCTGACAG TGAAGGAGAC GGAGGTGGTT 
TACATCACCC AGGCTCACCT TCACTTCACA GACCGAGAGC ACCCAGATGC TGACCTCACC 
TATGTCATTA CACAGCCCTG CTTCAGCCCG CTACATCCTG GCCTGATGGA TGCAGGACGT 
CTGTTTTACA CCGACAGCAC CAACGCCATG AAGAAGGACC ACACAGCGCC CGTGCTGAAG 
TCCTTCACTC AGCATGCTGT GAACCACATG AAGGTGGCCT TCATGCCGCC GGTCGAGGAC 
ATTGGGCCCG AGTCGCTCTT CGTCCAGTTC GTCTTCTCAG TCAGCGATCA CCACGGCGAC 
ACCGTCTCCG GCCTCATCTT CAACATCACG GTTACCCCTG TCGATGACCA GGCGCCAGAG 
GCTTTCACCA ACCTGCTTCG GGTGGAGGAG GGCGGCGCGG CCTTCGTGAC GGAGGAGCAC 
CTCTTGGTCC GGGACCGGGA CAGTCGAGAG GAGGCGCTGA GGGTGGAGAT TCGGAGCACG 
GCTCGTCACG GCCGGCTGGA GCTGTGGGGC CAAGCGCTGC AGCAGGGAGG CGGGTTTACC 
CTGCAGGAAC TGAGAGGGCT GGGCATCAGG TACATCCACG ATGACTCTGA GACCACTGAG 
GATGTCGTTG GTCTCAAGGT GTCGGACGGT CTGAACTCTG TGGACGTCGT CCTGCACGTT 
CAGGTGCTGC CGATGAACGA CGAGCCTCCT CAGCTGGGCG CCCGTCTGCA AGGGGGGCTG 
AGCTGCGAGG AAGGGGGGCG TGTCCAGGTG ATGGTGGACT ACCTGTCGGC CACCGACCGG 
GACAGCGACG ATTCCGCGCT GACCTACATG CTCGCCCGGA GACCGAGCAG GGGAGAGCTG 
CAGCGAGCCG GCGTGACTGT GGACAAGTTC TCCCAGCAGG ACGTCCTGCA GGGTCACATC 
TACTATGCCC ACACAGGCGG GGAGATCGGA CCTGAGCCGG TGTTCGACAC GGTCACCCTC 
ATCATCTCCG ACAGTGAGGC CAGAGGGCCG GACAGCTGCT GCCACGGGGA GGCCCCGCCC 
CCTCCTGTTC CTCTCCATGG TACACTTCCT GTTTACGATC TGAACATCAC AGTTCTTCCT 
GTCAACGATA AAGTCCCTGC TGTCACTCTG GGCGAGTCCA TGCTGGTGGT GGATGAAGGC 
TCTTCTATCT GTCTGTGCGG GGGGGTCCTC GGAGCGTCAG ACCCAGACAG TCCCCCTGAG 
CAGCTCGTCT TTCACCTGGA GACTCCTCCC CTTCACGGCT TCCTGGAGAA CACGCAGCCG 
ATGCCCGGCT CCGAAAAGAG CAACGCGGGA GTGCGAGTCG AATCCTTCAC TCTCAAACAC 
CTGACCTCTG GCTTCATCAA CTACGTGCAA TCGGAGCACA AAGGGGTGGA GCCAACTGTC 
GACCAGCTGT CCATCAGTGT GAGCGACGGG CTACATCGCT CCGCCCCCGT CTCCTTCTAC 
ATCATTATCA AACCAACCAA TGACGAAACA CCATCGCTGC TGCTCGCCAA CTTCACCGTA 
AAAGAGGGCG GGATGAGGGA GCTAATTCCA TCCATACTGG ATGGTTTTGA CTTGGACGCC 
CCACCTGAGC TGCTCACCTT CACTGTGGTG CAGCCGCCGG CTCACGGCAG CCTGATTAAC 
AGCATCCACG GCACGGAGGC GGGAGCTGAC CTCCTGCACA GGAGCCTCTC TGTCACCTCT 
TTCACTCTGC AGGAGCTCCG ACAAGGCATG AGGATCGTCT ACATGCACGA TGACACAGAA 
ACCCTGAAGG ACGCCGTGGC TCTGCGGCTG ACAGATGGTG TCCATGCAGT CGAGGGAACA 
GTTCAGGTCA CCGTGCTGCT GGTCAACGAC GAGAAACCAC GACTGCTCAA GAATGCTGGA 
CTGCAGGTGG ACTCAGGTGA GCTCAGGGTG ATCTCCAGTG TGGTTCTGGA GGCTGAGGAC 
CTGGACACGC CCCCAGAACA GGTGTACTAC TTCCTTAATG TTGCTCCGCG GTTTGGAAAA 
CTGCAGCTGA AGACGACGTC GGGGTGGAAC GAGCTGACGG CGGGTCAGAA CTTCACACAG 
GGCGACGTGG AGATGAACCG ACTGTGGTAC CAACACACCG CCGCCACCAA CGCAGCCGGC 
TTTAAAGGTC ACGACAGCTT CCGCTTCACC CTCAGCGACC TGGACAACGA GTCTCCGGCT 
CAGAGCTTCT TCATCTCTGT GCACGTGATG CAGAAAGGGG ACATCGTGGT GCTGAGCAGG 
CCAGTGCACC TGAGGGAGGG GCAGCGTGTC GTCCTGAACA CTGACATCCT GCTGGCATCA 
GACTCGACTG GCCGGCCCAA GGAGCTGGTC TACACGGTGT CTGACCCGCC GCGTCACGGC 
CTCGTCCACG CCGTCCACCG GCCTGGAGTC CCGCTACTCA GCTTCACCCA GCTGGACGTG 
GCTGCGCACC GAGTCTGCTA CACCCACGAC AACAGCCATG ATGCCGAGGC TGACTCCTTC 
AGTTTTGTTG TTACAAACGG TGACTCGTCT CGCAGCTCCA CCCTCCACTT CACCATCGAG 
CATGGTGACC GCATCCCGCC CACCCTCAGC CGCAACGTTG GCCTCCGGCT GCAGGATGGC 
TCCACAGTGA CCATCACTTC GGACCAGCTG CAGCTCACCG ACCCCGACAC TACCACCGCC 
AACCTGGGAT ACGTCATCAC GCAGCTGCCG CAGCACGGGA AGCTGCTGCT GAGAGGCACG 
CCGCTACCCT CGCCGCCCCG GTTCTCCCAG ACGAACGTGG ACGACCTCCA CCTGACTTAC 
CAACACGACC CGAGCAGCCC TGCGAAGATC GACGGGTTCT ACTTTATGCC GAGCGACGGA 
CACAACAGGG GCTACCTGGA GTTTGGGCAG CTGAGGGAGG AGCCTGCTGT GTTTGACATA 
CAGGTGGATT GGGTCGACCG GACGCCTCCC ACTCTGACCA CCAGGGGGAG TCCCAGCACC 
GTGGTGGATC TGGGTGGCGG CCGTTACGGA ATCTTCATCA CCAGCAGAGA GCTGCAGGCC 
TCAGACCCTG ACAGCCCCAC AGAGGAGCTC GAGTTCTCCA TCAGCAGACC TCCACTCTTA 
GGATACCTGG AGAACGCCGC CACAGGGTCG TACATCAAAG GTCGTTTCAC GCAGCGTGAC 
GTGGATCGGC GAGCCGTGAT GTTCGTCCTC CCCGCTGACG TGGAAGTGAC GTCTGACAGC 
TTCCTGTTTG TCCTCAGAGA TCCGGCAGGA AACAGCGCGT TGCCTCAGAC GCTGGAGCTG 
TCCTGGTCTC GAGTGGAGCT GTCGGCGACC TGCTACAGAA CCTGTGAGAC GGCCGGGATA 
CTTCAGATCC GCGTGCAGCG CAACGGGAGG AGCGCTGACC CTGCCTACGT CGCCATCCAG 
GTGGACGAAG GATCAGCCAA AGCTGGCAGA GATTTCACTC ACAGCACTGC GAGTCTCATC 
CAGTTCGATC CAGGTGTGAA GGTGAAAACC TGGAACATTT ACCTGATTGA CGACGGCCTG 
GAAGAAAACC ACGAGAGCTT CACGGTCACG CTGAGAAACC CCCAGAACGC CGTCCTGGGA 
CCAAGGAGCT CGGCCACCGT GGAGATCATT GACCCCCACC GAGGAAGCTG TGACCCAGAC 
GACCTGTGGG TAGAAGATGA TCAGCGCCCC CTTCCTCCTC CTCCTACTCC TCCCCCTCTC 
CCTCCTCAGC TCCCTGATCC TCTGCAGCAA GAGGAGGAAG AGGAGGTTCC GGTCATCAAT 
ATAGAGGCGG AGCTGCTGTG GGAGAACCAG CCTCACCCGC CCCGAGGAGA CGTCCCGAAT 
CGACATCACC TGGACTACAA AGAGGTGGAG CCACGAGACC CAGAGCCCCA CAGCAATGGC 
TACACCCCCC ACAGCAGCCA CCTAAACATT CAGCAGCACA CTGGAAGCAG TACTGGGAAC 
ACTGGGAGCA CGGTCCGTCT GTCTGCACAG AGGAGGCCTG AGGACGAGCA GGAGAAGGTT 
TGGACGTTCC ACAGTCTGAC TCCTCTCCGG CTGGAGGAGC TGCAACCGGG CGATGTCGAG 
TGGAGCTGGT CAGCTCACGG TCGTCCCCTG CAGGAACTCC AAGTTGGAGA TTCTCCTCAG 
ATGGCTCATC AAGCTCAGAA ACACCAACCC GAGCTCCGCC AGCACAAGGT GGGGAAGTCT 
GTGAGCAGCT CATGTCCTGA TGGCTGGACG CACCACAGGA GACGATGTTA CATCCTCAGC 
CCGTCCGTGG CGAGCTGGGC CAGCGCCGAC AGAACCTGCT CGCTGCTGTT TAACAGCAGC 
CTGACCGACG TGCACTCCAG GAGAGATGTG AGCTGGTTGT GGAGGTTTGC AGGGAAGAAG 
CCATTTTGGA TCGGTCTGTC TGGAGGTCCA GGACGCTCAT CGTGGGCTGA CAGTCGCTCG 
CTGTCTTCCT CCAGACTGAG GGGGGCGCCA GTGAGCCCCG GTGAGCCCGA GAACGACTCC 
AACTGCGTGC TGGTTCAAAA CCCGAGAAGC TGGATCTCCA CGAGCTGCTC TGCAGAAACT 
CAGCACTCAT TCATCTGTTC ATCGCCAGCT CAAACTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012776236.1
CDS	142..6603
Translation	MAAVLVSLLLLAVTGVCPRTLVVVNSAVEVSRGRSVFITRAELQISVDPSADCKVEVVMNEPVTQRVGRLSPQVFDCSFLEDEVKYVHDGSPLLDEDVVMLRVYRFTSSDTQVETVILRVRVVDSGAGVVALGSAPLVVPQFYGLSNAINGSVLNIRAAPDLVCTVRLLTADTSVPAAGRLVREEDSTQRKGRQATLCPGNSPCLHDTTEVHFLKTSCHEFLSSALKYQHLSPPSPEIDYVPVRVELREQASRALLESQAVWLPVLIHGAMQNQPPQATFMASFILEVDQFILTPLTTATLDAKDHETPQEKLVFNVTVPPAEGYITHLDDHTKPIRSFTWLDLHEMKVAYQPSNSSRGRRRNYEVEFQVIDGSYLTSPPIVVHVSIRTSETNAPRVSWNVGLDLLEGQSRPITWEQLQIVDSDNIDAVYLVAVDGPLHGRLSVRGGKAFMFRVRDLKEGTVVYHHSDSDSTRDHVVFRISDGRHSVRHKFPINILPKDDSPPFLINNVAVEVQEGGAVRLEEFMLLASDLDSSDDYILYQIVSAPRAGHLVRRTSAHETGSPVASFLQRDLIQGQIFYQHSGDEQFEDAFDFTLSDSHRPPNLSQTYTVVVHVFPVEDQVPVEVPGSVRSLTVKETEVVYITQAHLHFTDREHPDADLTYVITQPCFSPLHPGLMDAGRLFYTDSTNAMKKDHTAPVLKSFTQHAVNHMKVAFMPPVEDIGPESLFVQFVFSVSDHHGDTVSGLIFNITVTPVDDQAPEAFTNLLRVEEGGAAFVTEEHLLVRDRDSREEALRVEIRSTARHGRLELWGQALQQGGGFTLQELRGLGIRYIHDDSETTEDVVGLKVSDGLNSVDVVLHVQVLPMNDEPPQLGARLQGGLSCEEGGRVQVMVDYLSATDRDSDDSALTYMLARRPSRGELQRAGVTVDKFSQQDVLQGHIYYAHTGGEIGPEPVFDTVTLIISDSEARGPDSCCHGEAPPPPVPLHGTLPVYDLNITVLPVNDKVPAVTLGESMLVVDEGSSICLCGGVLGASDPDSPPEQLVFHLETPPLHGFLENTQPMPGSEKSNAGVRVESFTLKHLTSGFINYVQSEHKGVEPTVDQLSISVSDGLHRSAPVSFYIIIKPTNDETPSLLLANFTVKEGGMRELIPSILDGFDLDAPPELLTFTVVQPPAHGSLINSIHGTEAGADLLHRSLSVTSFTLQELRQGMRIVYMHDDTETLKDAVALRLTDGVHAVEGTVQVTVLLVNDEKPRLLKNAGLQVDSGELRVISSVVLEAEDLDTPPEQVYYFLNVAPRFGKLQLKTTSGWNELTAGQNFTQGDVEMNRLWYQHTAATNAAGFKGHDSFRFTLSDLDNESPAQSFFISVHVMQKGDIVVLSRPVHLREGQRVVLNTDILLASDSTGRPKELVYTVSDPPRHGLVHAVHRPGVPLLSFTQLDVAAHRVCYTHDNSHDAEADSFSFVVTNGDSSRSSTLHFTIEHGDRIPPTLSRNVGLRLQDGSTVTITSDQLQLTDPDTTTANLGYVITQLPQHGKLLLRGTPLPSPPRFSQTNVDDLHLTYQHDPSSPAKIDGFYFMPSDGHNRGYLEFGQLREEPAVFDIQVDWVDRTPPTLTTRGSPSTVVDLGGGRYGIFITSRELQASDPDSPTEELEFSISRPPLLGYLENAATGSYIKGRFTQRDVDRRAVMFVLPADVEVTSDSFLFVLRDPAGNSALPQTLELSWSRVELSATCYRTCETAGILQIRVQRNGRSADPAYVAIQVDEGSAKAGRDFTHSTASLIQFDPGVKVKTWNIYLIDDGLEENHESFTVTLRNPQNAVLGPRSSATVEIIDPHRGSCDPDDLWVEDDQRPLPPPPTPPPLPPQLPDPLQQEEEEEVPVINIEAELLWENQPHPPRGDVPNRHHLDYKEVEPRDPEPHSNGYTPHSSHLNIQQHTGSSTGNTGSTVRLSAQRRPEDEQEKVWTFHSLTPLRLEELQPGDVEWSWSAHGRPLQELQVGDSPQMAHQAQKHQPELRQHKVGKSVSSSCPDGWTHHRRRCYILSPSVASWASADRTCSLLFNSSLTDVHSRRDVSWLWRFAGKKPFWIGLSGGPGRSSWADSRSLSSSRLRGAPVSPGEPENDSNCVLVQNPRSWISTSCSAETQHSFICSSPAQTH

Target ORF information:

RefSeq Version	XM_012920782.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012920782.1

ORF Insert Sequence:

ATGGCTGCCG TCCTCGTGTC CCTGCTGCTG CTCGCGGTGA CGGGCGTTTG CCCTCGGACT 
CTGGTTGTGG TGAACTCTGC CGTGGAGGTC AGCAGAGGGC GCTCGGTTTT CATCACGAGG 
GCGGAGCTTC AGATCAGCGT GGACCCGAGC GCCGACTGTA AGGTGGAGGT GGTGATGAAC 
GAGCCCGTGA CTCAGAGGGT CGGGAGGCTG AGCCCACAGG TGTTTGACTG CAGCTTCCTG 
GAGGACGAGG TGAAGTATGT CCACGACGGC AGCCCTCTGC TGGACGAGGA CGTGGTGATG 
CTCAGAGTCT ACAGGTTCAC GTCCTCGGAC ACGCAGGTGG AGACGGTGAT CCTGAGGGTC 
CGGGTGGTGG ACTCTGGTGC GGGCGTGGTG GCACTCGGCA GCGCTCCGCT GGTCGTTCCC 
CAGTTCTACG GTTTATCCAA CGCCATCAAC GGCTCTGTGC TGAATATCAG GGCCGCGCCT 
GACCTTGTCT GCACCGTCAG GCTGTTGACC GCAGACACCA GCGTCCCCGC TGCGGGTCGG 
CTGGTGAGGG AGGAAGACTC CACGCAGAGG AAGGGTCGAC AGGCGACGCT CTGCCCAGGA 
AACAGTCCGT GTCTTCACGA CACCACCGAG GTCCACTTCC TCAAAACCAG CTGCCATGAA 
TTCCTGAGCT CTGCCCTCAA GTACCAGCAC CTCAGCCCGC CGTCCCCGGA GATCGACTAC 
GTCCCCGTCA GGGTGGAGCT GAGAGAGCAG GCGAGCAGAG CACTGCTGGA GTCACAGGCC 
GTGTGGCTGC CGGTTTTGAT CCACGGGGCC ATGCAGAACC AGCCCCCGCA GGCTACCTTC 
ATGGCCTCTT TCATCCTGGA GGTGGACCAG TTCATCCTCA CCCCGCTCAC CACTGCCACG 
CTGGACGCCA AGGACCACGA GACTCCGCAG GAGAAGCTGG TCTTCAATGT GACCGTCCCA 
CCCGCCGAGG GCTACATCAC ACACCTGGAC GACCACACCA AGCCCATCCG GTCCTTCACA 
TGGCTGGACC TTCACGAGAT GAAAGTGGCC TACCAGCCAT CCAACAGCAG CCGGGGGCGC 
CGCAGGAACT ATGAGGTGGA GTTTCAGGTG ATAGACGGCT CCTATCTGAC CAGCCCACCC 
ATCGTGGTCC ACGTCTCCAT CAGGACATCG GAAACGAATG CTCCGAGAGT CTCCTGGAAC 
GTGGGTCTAG ACCTGTTGGA GGGTCAGTCC CGACCAATCA CCTGGGAGCA GCTGCAGATA 
GTCGACAGCG ACAACATTGA CGCCGTTTAC CTGGTGGCCG TGGACGGACC CCTGCACGGG 
CGGCTCAGTG TCAGAGGCGG GAAGGCGTTT ATGTTCCGCG TGCGGGACCT GAAGGAGGGC 
ACAGTCGTCT ACCACCACTC CGACAGCGAC TCCACCCGCG ACCACGTCGT CTTCCGCATC 
AGCGACGGTC GTCACAGCGT CCGCCACAAG TTCCCCATCA ACATCCTCCC GAAGGACGAC 
TCTCCGCCCT TCCTCATCAA CAACGTGGCG GTGGAGGTGC AGGAGGGTGG GGCCGTGCGG 
CTCGAGGAGT TCATGCTGCT GGCCTCCGAC CTCGACTCCA GCGACGACTA CATCCTTTAT 
CAGATAGTCT CCGCCCCCCG GGCCGGGCAC CTGGTCAGGA GGACATCCGC CCATGAGACA 
GGGTCACCGG TGGCCAGCTT CCTGCAGAGG GACCTGATCC AGGGTCAGAT CTTCTACCAG 
CACTCGGGAG ATGAGCAGTT TGAGGATGCC TTCGACTTCA CGCTGTCTGA CAGCCACCGG 
CCGCCCAACC TGTCGCAGAC ATACACGGTG GTGGTGCACG TGTTCCCGGT GGAGGACCAG 
GTGCCAGTCG AAGTTCCCGG CAGCGTCCGC TCGCTGACAG TGAAGGAGAC GGAGGTGGTT 
TACATCACCC AGGCTCACCT TCACTTCACA GACCGAGAGC ACCCAGATGC TGACCTCACC 
TATGTCATTA CACAGCCCTG CTTCAGCCCG CTACATCCTG GCCTGATGGA TGCAGGACGT 
CTGTTTTACA CCGACAGCAC CAACGCCATG AAGAAGGACC ACACAGCGCC CGTGCTGAAG 
TCCTTCACTC AGCATGCTGT GAACCACATG AAGGTGGCCT TCATGCCGCC GGTCGAGGAC 
ATTGGGCCCG AGTCGCTCTT CGTCCAGTTC GTCTTCTCAG TCAGCGATCA CCACGGCGAC 
ACCGTCTCCG GCCTCATCTT CAACATCACG GTTACCCCTG TCGATGACCA GGCGCCAGAG 
GCTTTCACCA ACCTGCTTCG GGTGGAGGAG GGCGGCGCGG CCTTCGTGAC GGAGGAGCAC 
CTCTTGGTCC GGGACCGGGA CAGTCGAGAG GAGGCGCTGA GGGTGGAGAT TCGGAGCACG 
GCTCGTCACG GCCGGCTGGA GCTGTGGGGC CAAGCGCTGC AGCAGGGAGG CGGGTTTACC 
CTGCAGGAAC TGAGAGGGCT GGGCATCAGG TACATCCACG ATGACTCTGA GACCACTGAG 
GATGTCGTTG GTCTCAAGGT GTCGGACGGT CTGAACTCTG TGGACGTCGT CCTGCACGTT 
CAGGTGCTGC CGATGAACGA CGAGCCTCCT CAGCTGGGCG CCCGTCTGCA AGGGGGGCTG 
AGCTGCGAGG AAGGGGGGCG TGTCCAGGTG ATGGTGGACT ACCTGTCGGC CACCGACCGG 
GACAGCGACG ATTCCGCGCT GACCTACATG CTCGCCCGGA GACCGAGCAG GGGAGAGCTG 
CAGCGAGCCG GCGTGACTGT GGACAAGTTC TCCCAGCAGG ACGTCCTGCA GGGTCACATC 
TACTATGCCC ACACAGGCGG GGAGATCGGA CCTGAGCCGG TGTTCGACAC GGTCACCCTC 
ATCATCTCCG ACAGTGAGGC CAGAGGGCCG GACAGCTGCT GCCACGGGGA GGCCCCGCCC 
CCTCCTGTTC CTCTCCATGG TACACTTCCT GTTTACGATC TGAACATCAC AGTTCTTCCT 
GTCAACGATA AAGTCCCTGC TGTCACTCTG GGCGAGTCCA TGCTGGTGGT GGATGAAGGC 
TCTTCTATCT GTCTGTGCGG GGGGGTCCTC GGAGCGTCAG ACCCAGACAG TCCCCCTGAG 
CAGCTCGTCT TTCACCTGGA GACTCCTCCC CTTCACGGCT TCCTGGAGAA CACGCAGCCG 
ATGCCCGGCT CCGAAAAGAG CAACGCGGGA GTGCGAGTCG AATCCTTCAC TCTCAAACAC 
CTGACCTCTG GCTTCATCAA CTACGTGCAA TCGGAGCACA AAGGGGTGGA GCCAACTGTC 
GACCAGCTGT CCATCAGTGT GAGCGACGGG CTACATCGCT CCGCCCCCGT CTCCTTCTAC 
ATCATTATCA AACCAACCAA TGACGAAACA CCATCGCTGC TGCTCGCCAA CTTCACCGTA 
AAAGAGGGCG GGATGAGGGA GCTAATTCCA TCCATACTGG ATGGTTTTGA CTTGGACGCC 
CCACCTGAGC TGCTCACCTT CACTGTGGTG CAGCCGCCGG CTCACGGCAG CCTGATTAAC 
AGCATCCACG GCACGGAGGC GGGAGCTGAC CTCCTGCACA GGAGCCTCTC TGTCACCTCT 
TTCACTCTGC AGGAGCTCCG ACAAGGCATG AGGATCGTCT ACATGCACGA TGACACAGAA 
ACCCTGAAGG ACGCCGTGGC TCTGCGGCTG ACAGATGGTG TCCATGCAGT CGAGGGAACA 
GTTCAGGTCA CCGTGCTGCT GGTCAACGAC GAGAAACCAC GACTGCTCAA GAATGCTGGA 
CTGCAGGTGG ACTCAGGTGA GCTCAGGGTG ATCTCCAGTG TGGTTCTGGA GGCTGAGGAC 
CTGGACACGC CCCCAGAACA GGTGTACTAC TTCCTTAATG TTGCTCCGCG GTTTGGAAAA 
CTGCAGCTGA AGACGACGTC GGGGTGGAAC GAGCTGACGG CGGGTCAGAA CTTCACACAG 
GGCGACGTGG AGATGAACCG ACTGTGGTAC CAACACACCG CCGCCACCAA CGCAGCCGGC 
TTTAAAGGTC ACGACAGCTT CCGCTTCACC CTCAGCGACC TGGACAACGA GTCTCCGGCT 
CAGAGCTTCT TCATCTCTGT GCACGTGATG CAGAAAGGGG ACATCGTGGT GCTGAGCAGG 
CCAGTGCACC TGAGGGAGGG GCAGCGTGTC GTCCTGAACA CTGACATCCT GCTGGCATCA 
GACTCGACTG GCCGGCCCAA GGAGCTGGTC TACACGGTGT CTGACCCGCC GCGTCACGGC 
CTCGTCCACG CCGTCCACCG GCCTGGAGTC CCGCTACTCA GCTTCACCCA GCTGGACGTG 
GCTGCGCACC GAGTCTGCTA CACCCACGAC AACAGCCATG ATGCCGAGGC TGACTCCTTC 
AGTTTTGTTG TTACAAACGG TGACTCGTCT CGCAGCTCCA CCCTCCACTT CACCATCGAG 
CATGGTGACC GCATCCCGCC CACCCTCAGC CGCAACGTTG GCCTCCGGCT GCAGGATGGC 
TCCACAGTGA CCATCACTTC GGACCAGCTG CAGCTCACCG ACCCCGACAC TACCACCGCC 
AACCTGGGAT ACGTCATCAC GCAGCTGCCG CAGCACGGGA AGCTGCTGCT GAGAGGCACG 
CCGCTACCCT CGCCGCCCCG GTTCTCCCAG ACGAACGTGG ACGACCTCCA CCTGACTTAC 
CAACACGACC CGAGCAGCCC TGCGAAGATC GACGGGTTCT ACTTTATGCC GAGCGACGGA 
CACAACAGGG GCTACCTGGA GTTTGGGCAG CTGAGGGAGG AGCCTGCTGT GTTTGACATA 
CAGGTGGATT GGGTCGACCG GACGCCTCCC ACTCTGACCA CCAGGGGGAG TCCCAGCACC 
GTGGTGGATC TGGGTGGCGG CCGTTACGGA ATCTTCATCA CCAGCAGAGA GCTGCAGGCC 
TCAGACCCTG ACAGCCCCAC AGAGGAGCTC GAGTTCTCCA TCAGCAGACC TCCACTCTTA 
GGATACCTGG AGAACGCCGC CACAGGGTCG TACATCAAAG GTCGTTTCAC GCAGCGTGAC 
GTGGATCGGC GAGCCGTGAT GTTCGTCCTC CCCGCTGACG TGGAAGTGAC GTCTGACAGC 
TTCCTGTTTG TCCTCAGAGA TCCGGCAGGA AACAGCGCGT TGCCTCAGAC GCTGGAGCTG 
TCCTGGTCTC GAGTGGAGCT GTCGGCGACC TGCTACAGAA CCTGTGAGAC GGCCGGGATA 
CTTCAGATCC GCGTGCAGCG CAACGGGAGG AGCGCTGACC CTGCCTACGT CGCCATCCAG 
GTGGACGAAG GATCAGCCAA AGCTGGCAGA GATTTCACTC ACAGCACTGC GAGTCTCATC 
CAGTTCGATC CAGGTGTGAA GGTGAAAACC TGGAACATTT ACCTGATTGA CGACGGCCTG 
GAAGAAAACC ACGAGAGCTT CACGGTCACG CTGAGAAACC CCCAGAACGC CGTCCTGGGA 
CCAAGGAGCT CGGCCACCGT GGAGATCATT GACCCCCACC GAGGAAGCTG TGACCCAGAC 
GACCTGTGGG TAGAAGATGA TCAGCGCCCC CTTCCTCCTC CTCCTACTCC TCCCCCTCTC 
CCTCCTCAGC TCCCTGATCC TCTGCAGCAA GAGGAGGAAG AGGAGGTTCC GGTCATCAAT 
ATAGAGGCGG AGCTGCTGTG GGAGAACCAG CCTCACCCGC CCCGAGGAGA CGTCCCGAAT 
CGACATCACC TGGACTACAA AGAGGTGGAG CCACGAGACC CAGAGCCCCA CAGCAATGGC 
TACACCCCCC ACAGCAGCCA CCTAAACATT CAGCAGCACA CTGGAAGCAG TACTGGGAAC 
ACTGGGAGCA CGGTCCGTCT GTCTGCACAG AGGAGGCCTG AGGACGAGCA GGAGAAGGTT 
TGGACGTTCC ACAGTCTGAC TCCTCTCCGG CTGGAGGAGC TGCAACCGGG CGATGTCGAG 
TGGAGCTGGT CAGCTCACGG TCGTCCCCTG CAGGAACTCC AAGTTGGAGA TTCTCCTCAG 
ATGGCTCATC AAGCTCAGAA ACACCAACCC GAGCTCCGCC AGCACAAGGT GGGGAAGTCT 
GTGAGCAGCT CATGTCCTGA TGGCTGGACG CACCACAGGA GACGATGTTA CATCCTCAGC 
CCGTCCGTGG CGAGCTGGGC CAGCGCCGAC AGAACCTGCT CGCTGCTGTT TAACAGCAGC 
CTGACCGACG TGCACTCCAG GAGAGATGTG AGCTGGTTGT GGAGGTTTGC AGGGAAGAAG 
CCATTTTGGA TCGGTCTGTC TGGAGGTCCA GGACGCTCAT CGTGGGCTGA CAGTCGCTCG 
CTGTCTTCCT CCAGACTGAG GGGGGCGCCA GTGAGCCCCG GTGAGCCCGA GAACGACTCC 
AACTGCGTGC TGGTTCAAAA CCCGAGAAGC TGGATCTCCA CGAGCTGCTC TGCAGAAACT 
CAGCACTCAT TCATCTGTTC ATCGCCAGCT CAAACTCACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101486624 ( XM_004557000.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004557057.1 Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), transcript variant X4, mRNA.

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CloneID	OMa02958
Clone ID Related Accession (Same CDS sequence)	XM_004557000.1
Accession Version	XM_004557000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	FRAS1-related extracellular matrix protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014444631.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCG TCCTCGTGTC CCTGCTGCTG CTCGCGGTGA CGGGCGTTTG CCCTCGGACT 
CTGGTTGTGG TGAACTCTGC CGTGGAGGTC AGCAGAGGGC GCTCGGTTTT CATCACGAGG 
GCGGAGCTTC AGATCAGCGT GGACCCGAGC GCCGACTGTA AGGTGGAGGT GGTGATGAAC 
GAGCCCGTGA CTCAGAGGGT CGGGAGGCTG AGCCCACAGG TGTTTGACTG CAGCTTCCTG 
GAGGACGAGG TGAAGTATGT CCACGACGGC AGCCCTCTGC TGGACGAGGA CGTGGTGATG 
CTCAGAGTCT ACAGGTTCAC GTCCTCGGAC ACGCAGGTGG AGACGGTGAT CCTGAGGGTC 
CGGGTGGTGG ACTCTGGTGC GGGCGTGGTG GCACTCGGCA GCGCTCCGCT GGTCGTTCCC 
CAGTTCTACG GTTTATCCAA CGCCATCAAC GGCTCTGTGC TGAATATCAG GGCCGCGCCT 
GACCTTGTCT GCACCGTCAG GCTGTTGACC GCAGACACCA GCGTCCCCGC TGCGGGTCGG 
CTGGTGAGGG AGGAAGACTC CACGCAGAGG AAGGGTCGAC AGGCGACGCT CTGCCCAGGA 
AACAGTCCGT GTCTTCACGA CACCACCGAG GTCCACTTCC TCAAAACCAG CTGCCATGAA 
TTCCTGAGCT CTGCCCTCAA GTACCAGCAC CTCAGCCCGC CGTCCCCGGA GATCGACTAC 
GTCCCCGTCA GGGTGGAGCT GAGAGAGCAG GCGAGCAGAG CACTGCTGGA GTCACAGGCC 
GTGTGGCTGC CGGTTTTGAT CCACGGGGCC ATGCAGAACC AGCCCCCGCA GGCTACCTTC 
ATGGCCTCTT TCATCCTGGA GGTGGACCAG TTCATCCTCA CCCCGCTCAC CACTGCCACG 
CTGGACGCCA AGGACCACGA GACTCCGCAG GAGAAGCTGG TCTTCAATGT GACCGTCCCA 
CCCGCCGAGG GCTACATCAC ACACCTGGAC GACCACACCA AGCCCATCCG GTCCTTCACA 
TGGCTGGACC TTCACGAGAT GAAAGTGGCC TACCAGCCAT CCAACAGCAG CCGGGGGCGC 
CGCAGGAACT ATGAGGTGGA GTTTCAGGTG ATAGACGGCT CCTATCTGAC CAGCCCACCC 
ATCGTGGTCC ACGTCTCCAT CAGGACATCG GAAACGAATG CTCCGAGAGT CTCCTGGAAC 
GTGGGTCTAG ACCTGTTGGA GGGTCAGTCC CGACCAATCA CCTGGGAGCA GCTGCAGATA 
GTCGACAGCG ACAACATTGA CGCCGTTTAC CTGGTGGCCG TGGACGGACC CCTGCACGGG 
CGGCTCAGTG TCAGAGGCGG GAAGGCGTTT ATGTTCCGCG TGCGGGACCT GAAGGAGGGC 
ACAGTCGTCT ACCACCACTC CGACAGCGAC TCCACCCGCG ACCACGTCGT CTTCCGCATC 
AGCGACGGTC GTCACAGCGT CCGCCACAAG TTCCCCATCA ACATCCTCCC GAAGGACGAC 
TCTCCGCCCT TCCTCATCAA CAACGTGGCG GTGGAGGTGC AGGAGGGTGG GGCCGTGCGG 
CTCGAGGAGT TCATGCTGCT GGCCTCCGAC CTCGACTCCA GCGACGACTA CATCCTTTAT 
CAGATAGTCT CCGCCCCCCG GGCCGGGCAC CTGGTCAGGA GGACATCCGC CCATGAGACA 
GGGTCACCGG TGGCCAGCTT CCTGCAGAGG GACCTGATCC AGGGTCAGAT CTTCTACCAG 
CACTCGGGAG ATGAGCAGTT TGAGGATGCC TTCGACTTCA CGCTGTCTGA CAGCCACCGG 
CCGCCCAACC TGTCGCAGAC ATACACGGTG GTGGTGCACG TGTTCCCGGT GGAGGACCAG 
GTGCCAGTCG AAGTTCCCGG CAGCGTCCGC TCGCTGACAG TGAAGGAGAC GGAGGTGGTT 
TACATCACCC AGGCTCACCT TCACTTCACA GACCGAGAGC ACCCAGATGC TGACCTCACC 
TATGTCATTA CACAGCCCTG CTTCAGCCCG CTACATCCTG GCCTGATGGA TGCAGGACGT 
CTGTTTTACA CCGACAGCAC CAACGCCATG AAGAAGGACC ACACAGCGCC CGTGCTGAAG 
TCCTTCACTC AGCATGCTGT GAACCACATG AAGGTGGCCT TCATGCCGCC GGTCGAGGAC 
ATTGGGCCCG AGTCGCTCTT CGTCCAGTTC GTCTTCTCAG TCAGCGATCA CCACGGCGAC 
ACCGTCTCCG GCCTCATCTT CAACATCACG GTTACCCCTG TCGATGACCA GGCGCCAGAG 
GCTTTCACCA ACCTGCTTCG GGTGGAGGAG GGCGGCGCGG CCTTCGTGAC GGAGGAGCAC 
CTCTTGGTCC GGGACCGGGA CAGTCGAGAG GAGGCGCTGA GGGTGGAGAT TCGGAGCACG 
GCTCGTCACG GCCGGCTGGA GCTGTGGGGC CAAGCGCTGC AGCAGGGAGG CGGGTTTACC 
CTGCAGGAAC TGAGAGGGCT GGGCATCAGG TACATCCACG ATGACTCTGA GACCACTGAG 
GATGTCGTTG GTCTCAAGGT GTCGGACGGT CTGAACTCTG TGGACGTCGT CCTGCACGTT 
CAGGTGCTGC CGATGAACGA CGAGCCTCCT CAGCTGGGCG CCCGTCTGCA AGGGGGGCTG 
AGCTGCGAGG AAGGGGGGCG TGTCCAGGTG ATGGTGGACT ACCTGTCGGC CACCGACCGG 
GACAGCGACG ATTCCGCGCT GACCTACATG CTCGCCCGGA GACCGAGCAG GGGAGAGCTG 
CAGCGAGCCG GCGTGACTGT GGACAAGTTC TCCCAGCAGG ACGTCCTGCA GGGTCACATC 
TACTATGCCC ACACAGGCGG GGAGATCGGA CCTGAGCCGG TGTTCGACAC GGTCACCCTC 
ATCATCTCCG ACAGTGAGGC CAGAGGGCCG GACAGCTGCT GCCACGGGGA GGCCCCGCCC 
CCTCCTGTTC CTCTCCATGG CGAGTCCATG CTGGTGGTGG ATGAAGGCTC TTCTATCTGT 
CTGTGCGGGG GGGTCCTCGG AGCGTCAGAC CCAGACAGTC CCCCTGAGCA GCTCGTCTTT 
CACCTGGAGA CTCCTCCCCT TCACGGCTTC CTGGAGAACA CGCAGCCGAT GCCCGGCTCC 
GAAAAGAGCA ACGCGGGAGT GCGAGTCGAA TCCTTCACTC TCAAACACCT GACCTCTGGC 
TTCATCAACT ACGTGCAATC GGAGCACAAA GGGGTGGAGC CAACTGTCGA CCAGCTGTCC 
ATCAGTGTGA GCGACGGGCT ACATCGCTCC GCCCCCGTCT CCTTCTACAT CATTATCAAA 
CCAACCAATG ACGAAACACC ATCGCTGCTG CTCGCCAACT TCACCGTAAA AGAGGGCGGG 
ATGAGGGAGC TAATTCCATC CATACTGGAT GGTTTTGACT TGGACGCCCC ACCTGAGCTG 
CTCACCTTCA CTGTGGTGCA GCCGCCGGCT CACGGCAGCC TGATTAACAG CATCCACGGC 
ACGGAGGCGG GAGCTGACCT CCTGCACAGG AGCCTCTCTG TCACCTCTTT CACTCTGCAG 
GAGCTCCGAC AAGGCATGAG GATCGTCTAC ATGCACGATG ACACAGAAAC CCTGAAGGAC 
GCCGTGGCTC TGCGGCTGAC AGATGGTGTC CATGCAGTCG AGGGAACAGT TCAGGTCACC 
GTGCTGCTGG TCAACGACGA GAAACCACGA CTGCTCAAGA ATGCTGGACT GCAGGTGGAC 
TCAGGTGAGC TCAGGGTGAT CTCCAGTGTG GTTCTGGAGG CTGAGGACCT GGACACGCCC 
CCAGAACAGG TGTACTACTT CCTTAATGTT GCTCCGCGGT TTGGAAAACT GCAGCTGAAG 
ACGACGTCGG GGTGGAACGA GCTGACGGCG GGTCAGAACT TCACACAGGG CGACGTGGAG 
ATGAACCGAC TGTGGTACCA ACACACCGCC GCCACCAACG CAGCCGGCTT TAAAGGTCAC 
GACAGCTTCC GCTTCACCCT CAGCGACCTG GACAACGAGT CTCCGGCTCA GAGCTTCTTC 
ATCTCTGTGC ACGTGATGCA GAAAGGGGAC ATCGTGGTGC TGAGCAGGCC AGTGCACCTG 
AGGGAGGGGC AGCGTGTCGT CCTGAACACT GACATCCTGC TGGCATCAGA CTCGACTGGC 
CGGCCCAAGG AGCTGGTCTA CACGGTGTCT GACCCGCCGC GTCACGGCCT CGTCCACGCC 
GTCCACCGGC CTGGAGTCCC GCTACTCAGC TTCACCCAGC TGGACGTGGC TGCGCACCGA 
GTCTGCTACA CCCACGACAA CAGCCATGAT GCCGAGGCTG ACTCCTTCAG TTTTGTTGTT 
ACAAACGGTG ACTCGTCTCG CAGCTCCACC CTCCACTTCA CCATCGAGCA TGGTGACCGC 
ATCCCGCCCA CCCTCAGCCG CAACGTTGGC CTCCGGCTGC AGGATGGCTC CACAGTGACC 
ATCACTTCGG ACCAGCTGCA GCTCACCGAC CCCGACACTA CCACCGCCAA CCTGGGATAC 
GTCATCACGC AGCTGCCGCA GCACGGGAAG CTGCTGCTGA GAGGCACGCC GCTACCCTCG 
CCGCCCCGGT TCTCCCAGAC GAACGTGGAC GACCTCCACC TGACTTACCA ACACGACCCG 
AGCAGCCCTG CGAAGATCGA CGGGTTCTAC TTTATGCCGA GCGACGGACA CAACAGGGGC 
TACCTGGAGT TTGGGCAGCT GAGGGAGGAG CCTGCTGTGT TTGACATACA GGTGGATTGG 
GTCGACCGGA CGCCTCCCAC TCTGACCACC AGGGGGAGTC CCAGCACCGT GGTGGATCTG 
GGTGGCGGCC GTTACGGAAT CTTCATCACC AGCAGAGAGC TGCAGGCCTC AGACCCTGAC 
AGCCCCACAG AGGAGCTCGA GTTCTCCATC AGCAGACCTC CACTCTTAGG ATACCTGGAG 
AACGCCGCCA CAGGGTCGTA CATCAAAGGT CGTTTCACGC AGCGTGACGT GGATCGGCGA 
GCCGTGATGT TCGTCCTCCC CGCTGACGTG GAAGTGACGT CTGACAGCTT CCTGTTTGTC 
CTCAGAGATC CGGCAGGAAA CAGCGCGTTG CCTCAGACGC TGGAGCTGTC CTGGTCTCGA 
GTGGAGCTGT CGGCGACCTG CTACAGAACC TGTGAGACGG CCGGGATACT TCAGATCCGC 
GTGCAGCGCA ACGGGAGGAG CGCTGACCCT GCCTACGTCG CCATCCAGGT GGACGAAGGA 
TCAGCCAAAG CTGGCAGAGA TTTCACTCAC AGCACTGCGA GTCTCATCCA GTTCGATCCA 
GGTGTGAAGG TGAAAACCTG GAACATTTAC CTGATTGACG ACGGCCTGGA AGAAAACCAC 
GAGAGCTTCA CGGTCACGCT GAGAAACCCC CAGAACGCCG TCCTGGGACC AAGGAGCTCG 
GCCACCGTGG AGATCATTGA CCCCCACCGA GGAAGCTGTG ACCCAGACGA CCTGTGGGTA 
GAAGATGATC AGCGCCCCCT TCCTCCTCCT CCTACTCCTC CCCCTCTCCC TCCTCAGCTC 
CCTGATCCTC TGCAGCAAGA GGAGGAAGAG GAGGTTCCGG TCATCAATAT AGAGGCGGAG 
CTGCTGTGGG AGAACCAGCC TCACCCGCCC CGAGGAGACG TCCCGAATCG ACATCACCTG 
GACTACAAAG AGGTGGAGCC ACGAGACCCA GAGCCCCACA GCAATGGCTA CACCCCCCAC 
AGCAGCCACC TAAACATTCA GCAGCACACT GGAAGCAGTA CTGGGAACAC TGGGAGCACG 
GTCCGTCTGT CTGCACAGAG GAGGCCTGAG GACGAGCAGG AGAAGGTTTG GACGTTCCAC 
AGTCTGACTC CTCTCCGGCT GGAGGAGCTG CAACCGGGCG ATGTCGAGTG GAGCTGGTCA 
GCTCACGGTC GTCCCCTGCA GGAACTCCAA GTTGGAGATT CTCCTCAGAT GGCTCATCAA 
GCTCAGAAAC ACCAACCCGA GCTCCGCCAG CACAAGGTGG GGAAGTCTGT GAGCAGCTCA 
TGTCCTGATG GCTGGACGCA CCACAGGAGA CGATGTTACA TCCTCAGCCC GTCCGTGGCG 
AGCTGGGCCA GCGCCGACAG AACCTGCTCG CTGCTGTTTA ACAGCAGCCT GACCGACGTG 
CACTCCAGGA GAGATGTGAG CTGGTTGTGG AGGTTTGCAG GGAAGAAGCC ATTTTGGATC 
GGTCTGTCTG GAGGTCCAGG ACGCTCATCG TGGGCTGACA GTCGCTCGCT GTCTTCCTCC 
AGACTGAGGG GGGCGCCAGT GAGCCCCGGT GAGCCCGAGA ACGACTCCAA CTGCGTGCTG 
GTTCAAAACC CGAGAAGCTG GATCTCCACG AGCTGCTCTG CAGAAACTCA GCACTCATTC 
ATCTGTTCAT CGCCAGCTCA AACTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004557057.1
CDS	303..6692
Translation	MAAVLVSLLLLAVTGVCPRTLVVVNSAVEVSRGRSVFITRAELQISVDPSADCKVEVVMNEPVTQRVGRLSPQVFDCSFLEDEVKYVHDGSPLLDEDVVMLRVYRFTSSDTQVETVILRVRVVDSGAGVVALGSAPLVVPQFYGLSNAINGSVLNIRAAPDLVCTVRLLTADTSVPAAGRLVREEDSTQRKGRQATLCPGNSPCLHDTTEVHFLKTSCHEFLSSALKYQHLSPPSPEIDYVPVRVELREQASRALLESQAVWLPVLIHGAMQNQPPQATFMASFILEVDQFILTPLTTATLDAKDHETPQEKLVFNVTVPPAEGYITHLDDHTKPIRSFTWLDLHEMKVAYQPSNSSRGRRRNYEVEFQVIDGSYLTSPPIVVHVSIRTSETNAPRVSWNVGLDLLEGQSRPITWEQLQIVDSDNIDAVYLVAVDGPLHGRLSVRGGKAFMFRVRDLKEGTVVYHHSDSDSTRDHVVFRISDGRHSVRHKFPINILPKDDSPPFLINNVAVEVQEGGAVRLEEFMLLASDLDSSDDYILYQIVSAPRAGHLVRRTSAHETGSPVASFLQRDLIQGQIFYQHSGDEQFEDAFDFTLSDSHRPPNLSQTYTVVVHVFPVEDQVPVEVPGSVRSLTVKETEVVYITQAHLHFTDREHPDADLTYVITQPCFSPLHPGLMDAGRLFYTDSTNAMKKDHTAPVLKSFTQHAVNHMKVAFMPPVEDIGPESLFVQFVFSVSDHHGDTVSGLIFNITVTPVDDQAPEAFTNLLRVEEGGAAFVTEEHLLVRDRDSREEALRVEIRSTARHGRLELWGQALQQGGGFTLQELRGLGIRYIHDDSETTEDVVGLKVSDGLNSVDVVLHVQVLPMNDEPPQLGARLQGGLSCEEGGRVQVMVDYLSATDRDSDDSALTYMLARRPSRGELQRAGVTVDKFSQQDVLQGHIYYAHTGGEIGPEPVFDTVTLIISDSEARGPDSCCHGEAPPPPVPLHGESMLVVDEGSSICLCGGVLGASDPDSPPEQLVFHLETPPLHGFLENTQPMPGSEKSNAGVRVESFTLKHLTSGFINYVQSEHKGVEPTVDQLSISVSDGLHRSAPVSFYIIIKPTNDETPSLLLANFTVKEGGMRELIPSILDGFDLDAPPELLTFTVVQPPAHGSLINSIHGTEAGADLLHRSLSVTSFTLQELRQGMRIVYMHDDTETLKDAVALRLTDGVHAVEGTVQVTVLLVNDEKPRLLKNAGLQVDSGELRVISSVVLEAEDLDTPPEQVYYFLNVAPRFGKLQLKTTSGWNELTAGQNFTQGDVEMNRLWYQHTAATNAAGFKGHDSFRFTLSDLDNESPAQSFFISVHVMQKGDIVVLSRPVHLREGQRVVLNTDILLASDSTGRPKELVYTVSDPPRHGLVHAVHRPGVPLLSFTQLDVAAHRVCYTHDNSHDAEADSFSFVVTNGDSSRSSTLHFTIEHGDRIPPTLSRNVGLRLQDGSTVTITSDQLQLTDPDTTTANLGYVITQLPQHGKLLLRGTPLPSPPRFSQTNVDDLHLTYQHDPSSPAKIDGFYFMPSDGHNRGYLEFGQLREEPAVFDIQVDWVDRTPPTLTTRGSPSTVVDLGGGRYGIFITSRELQASDPDSPTEELEFSISRPPLLGYLENAATGSYIKGRFTQRDVDRRAVMFVLPADVEVTSDSFLFVLRDPAGNSALPQTLELSWSRVELSATCYRTCETAGILQIRVQRNGRSADPAYVAIQVDEGSAKAGRDFTHSTASLIQFDPGVKVKTWNIYLIDDGLEENHESFTVTLRNPQNAVLGPRSSATVEIIDPHRGSCDPDDLWVEDDQRPLPPPPTPPPLPPQLPDPLQQEEEEEVPVINIEAELLWENQPHPPRGDVPNRHHLDYKEVEPRDPEPHSNGYTPHSSHLNIQQHTGSSTGNTGSTVRLSAQRRPEDEQEKVWTFHSLTPLRLEELQPGDVEWSWSAHGRPLQELQVGDSPQMAHQAQKHQPELRQHKVGKSVSSSCPDGWTHHRRRCYILSPSVASWASADRTCSLLFNSSLTDVHSRRDVSWLWRFAGKKPFWIGLSGGPGRSSWADSRSLSSSRLRGAPVSPGEPENDSNCVLVQNPRSWISTSCSAETQHSFICSSPAQTH

Target ORF information:

RefSeq Version	XM_004557000.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004557000.1

ORF Insert Sequence:

ATGGCTGCCG TCCTCGTGTC CCTGCTGCTG CTCGCGGTGA CGGGCGTTTG CCCTCGGACT 
CTGGTTGTGG TGAACTCTGC CGTGGAGGTC AGCAGAGGGC GCTCGGTTTT CATCACGAGG 
GCGGAGCTTC AGATCAGCGT GGACCCGAGC GCCGACTGTA AGGTGGAGGT GGTGATGAAC 
GAGCCCGTGA CTCAGAGGGT CGGGAGGCTG AGCCCACAGG TGTTTGACTG CAGCTTCCTG 
GAGGACGAGG TGAAGTATGT CCACGACGGC AGCCCTCTGC TGGACGAGGA CGTGGTGATG 
CTCAGAGTCT ACAGGTTCAC GTCCTCGGAC ACGCAGGTGG AGACGGTGAT CCTGAGGGTC 
CGGGTGGTGG ACTCTGGTGC GGGCGTGGTG GCACTCGGCA GCGCTCCGCT GGTCGTTCCC 
CAGTTCTACG GTTTATCCAA CGCCATCAAC GGCTCTGTGC TGAATATCAG GGCCGCGCCT 
GACCTTGTCT GCACCGTCAG GCTGTTGACC GCAGACACCA GCGTCCCCGC TGCGGGTCGG 
CTGGTGAGGG AGGAAGACTC CACGCAGAGG AAGGGTCGAC AGGCGACGCT CTGCCCAGGA 
AACAGTCCGT GTCTTCACGA CACCACCGAG GTCCACTTCC TCAAAACCAG CTGCCATGAA 
TTCCTGAGCT CTGCCCTCAA GTACCAGCAC CTCAGCCCGC CGTCCCCGGA GATCGACTAC 
GTCCCCGTCA GGGTGGAGCT GAGAGAGCAG GCGAGCAGAG CACTGCTGGA GTCACAGGCC 
GTGTGGCTGC CGGTTTTGAT CCACGGGGCC ATGCAGAACC AGCCCCCGCA GGCTACCTTC 
ATGGCCTCTT TCATCCTGGA GGTGGACCAG TTCATCCTCA CCCCGCTCAC CACTGCCACG 
CTGGACGCCA AGGACCACGA GACTCCGCAG GAGAAGCTGG TCTTCAATGT GACCGTCCCA 
CCCGCCGAGG GCTACATCAC ACACCTGGAC GACCACACCA AGCCCATCCG GTCCTTCACA 
TGGCTGGACC TTCACGAGAT GAAAGTGGCC TACCAGCCAT CCAACAGCAG CCGGGGGCGC 
CGCAGGAACT ATGAGGTGGA GTTTCAGGTG ATAGACGGCT CCTATCTGAC CAGCCCACCC 
ATCGTGGTCC ACGTCTCCAT CAGGACATCG GAAACGAATG CTCCGAGAGT CTCCTGGAAC 
GTGGGTCTAG ACCTGTTGGA GGGTCAGTCC CGACCAATCA CCTGGGAGCA GCTGCAGATA 
GTCGACAGCG ACAACATTGA CGCCGTTTAC CTGGTGGCCG TGGACGGACC CCTGCACGGG 
CGGCTCAGTG TCAGAGGCGG GAAGGCGTTT ATGTTCCGCG TGCGGGACCT GAAGGAGGGC 
ACAGTCGTCT ACCACCACTC CGACAGCGAC TCCACCCGCG ACCACGTCGT CTTCCGCATC 
AGCGACGGTC GTCACAGCGT CCGCCACAAG TTCCCCATCA ACATCCTCCC GAAGGACGAC 
TCTCCGCCCT TCCTCATCAA CAACGTGGCG GTGGAGGTGC AGGAGGGTGG GGCCGTGCGG 
CTCGAGGAGT TCATGCTGCT GGCCTCCGAC CTCGACTCCA GCGACGACTA CATCCTTTAT 
CAGATAGTCT CCGCCCCCCG GGCCGGGCAC CTGGTCAGGA GGACATCCGC CCATGAGACA 
GGGTCACCGG TGGCCAGCTT CCTGCAGAGG GACCTGATCC AGGGTCAGAT CTTCTACCAG 
CACTCGGGAG ATGAGCAGTT TGAGGATGCC TTCGACTTCA CGCTGTCTGA CAGCCACCGG 
CCGCCCAACC TGTCGCAGAC ATACACGGTG GTGGTGCACG TGTTCCCGGT GGAGGACCAG 
GTGCCAGTCG AAGTTCCCGG CAGCGTCCGC TCGCTGACAG TGAAGGAGAC GGAGGTGGTT 
TACATCACCC AGGCTCACCT TCACTTCACA GACCGAGAGC ACCCAGATGC TGACCTCACC 
TATGTCATTA CACAGCCCTG CTTCAGCCCG CTACATCCTG GCCTGATGGA TGCAGGACGT 
CTGTTTTACA CCGACAGCAC CAACGCCATG AAGAAGGACC ACACAGCGCC CGTGCTGAAG 
TCCTTCACTC AGCATGCTGT GAACCACATG AAGGTGGCCT TCATGCCGCC GGTCGAGGAC 
ATTGGGCCCG AGTCGCTCTT CGTCCAGTTC GTCTTCTCAG TCAGCGATCA CCACGGCGAC 
ACCGTCTCCG GCCTCATCTT CAACATCACG GTTACCCCTG TCGATGACCA GGCGCCAGAG 
GCTTTCACCA ACCTGCTTCG GGTGGAGGAG GGCGGCGCGG CCTTCGTGAC GGAGGAGCAC 
CTCTTGGTCC GGGACCGGGA CAGTCGAGAG GAGGCGCTGA GGGTGGAGAT TCGGAGCACG 
GCTCGTCACG GCCGGCTGGA GCTGTGGGGC CAAGCGCTGC AGCAGGGAGG CGGGTTTACC 
CTGCAGGAAC TGAGAGGGCT GGGCATCAGG TACATCCACG ATGACTCTGA GACCACTGAG 
GATGTCGTTG GTCTCAAGGT GTCGGACGGT CTGAACTCTG TGGACGTCGT CCTGCACGTT 
CAGGTGCTGC CGATGAACGA CGAGCCTCCT CAGCTGGGCG CCCGTCTGCA AGGGGGGCTG 
AGCTGCGAGG AAGGGGGGCG TGTCCAGGTG ATGGTGGACT ACCTGTCGGC CACCGACCGG 
GACAGCGACG ATTCCGCGCT GACCTACATG CTCGCCCGGA GACCGAGCAG GGGAGAGCTG 
CAGCGAGCCG GCGTGACTGT GGACAAGTTC TCCCAGCAGG ACGTCCTGCA GGGTCACATC 
TACTATGCCC ACACAGGCGG GGAGATCGGA CCTGAGCCGG TGTTCGACAC GGTCACCCTC 
ATCATCTCCG ACAGTGAGGC CAGAGGGCCG GACAGCTGCT GCCACGGGGA GGCCCCGCCC 
CCTCCTGTTC CTCTCCATGG CGAGTCCATG CTGGTGGTGG ATGAAGGCTC TTCTATCTGT 
CTGTGCGGGG GGGTCCTCGG AGCGTCAGAC CCAGACAGTC CCCCTGAGCA GCTCGTCTTT 
CACCTGGAGA CTCCTCCCCT TCACGGCTTC CTGGAGAACA CGCAGCCGAT GCCCGGCTCC 
GAAAAGAGCA ACGCGGGAGT GCGAGTCGAA TCCTTCACTC TCAAACACCT GACCTCTGGC 
TTCATCAACT ACGTGCAATC GGAGCACAAA GGGGTGGAGC CAACTGTCGA CCAGCTGTCC 
ATCAGTGTGA GCGACGGGCT ACATCGCTCC GCCCCCGTCT CCTTCTACAT CATTATCAAA 
CCAACCAATG ACGAAACACC ATCGCTGCTG CTCGCCAACT TCACCGTAAA AGAGGGCGGG 
ATGAGGGAGC TAATTCCATC CATACTGGAT GGTTTTGACT TGGACGCCCC ACCTGAGCTG 
CTCACCTTCA CTGTGGTGCA GCCGCCGGCT CACGGCAGCC TGATTAACAG CATCCACGGC 
ACGGAGGCGG GAGCTGACCT CCTGCACAGG AGCCTCTCTG TCACCTCTTT CACTCTGCAG 
GAGCTCCGAC AAGGCATGAG GATCGTCTAC ATGCACGATG ACACAGAAAC CCTGAAGGAC 
GCCGTGGCTC TGCGGCTGAC AGATGGTGTC CATGCAGTCG AGGGAACAGT TCAGGTCACC 
GTGCTGCTGG TCAACGACGA GAAACCACGA CTGCTCAAGA ATGCTGGACT GCAGGTGGAC 
TCAGGTGAGC TCAGGGTGAT CTCCAGTGTG GTTCTGGAGG CTGAGGACCT GGACACGCCC 
CCAGAACAGG TGTACTACTT CCTTAATGTT GCTCCGCGGT TTGGAAAACT GCAGCTGAAG 
ACGACGTCGG GGTGGAACGA GCTGACGGCG GGTCAGAACT TCACACAGGG CGACGTGGAG 
ATGAACCGAC TGTGGTACCA ACACACCGCC GCCACCAACG CAGCCGGCTT TAAAGGTCAC 
GACAGCTTCC GCTTCACCCT CAGCGACCTG GACAACGAGT CTCCGGCTCA GAGCTTCTTC 
ATCTCTGTGC ACGTGATGCA GAAAGGGGAC ATCGTGGTGC TGAGCAGGCC AGTGCACCTG 
AGGGAGGGGC AGCGTGTCGT CCTGAACACT GACATCCTGC TGGCATCAGA CTCGACTGGC 
CGGCCCAAGG AGCTGGTCTA CACGGTGTCT GACCCGCCGC GTCACGGCCT CGTCCACGCC 
GTCCACCGGC CTGGAGTCCC GCTACTCAGC TTCACCCAGC TGGACGTGGC TGCGCACCGA 
GTCTGCTACA CCCACGACAA CAGCCATGAT GCCGAGGCTG ACTCCTTCAG TTTTGTTGTT 
ACAAACGGTG ACTCGTCTCG CAGCTCCACC CTCCACTTCA CCATCGAGCA TGGTGACCGC 
ATCCCGCCCA CCCTCAGCCG CAACGTTGGC CTCCGGCTGC AGGATGGCTC CACAGTGACC 
ATCACTTCGG ACCAGCTGCA GCTCACCGAC CCCGACACTA CCACCGCCAA CCTGGGATAC 
GTCATCACGC AGCTGCCGCA GCACGGGAAG CTGCTGCTGA GAGGCACGCC GCTACCCTCG 
CCGCCCCGGT TCTCCCAGAC GAACGTGGAC GACCTCCACC TGACTTACCA ACACGACCCG 
AGCAGCCCTG CGAAGATCGA CGGGTTCTAC TTTATGCCGA GCGACGGACA CAACAGGGGC 
TACCTGGAGT TTGGGCAGCT GAGGGAGGAG CCTGCTGTGT TTGACATACA GGTGGATTGG 
GTCGACCGGA CGCCTCCCAC TCTGACCACC AGGGGGAGTC CCAGCACCGT GGTGGATCTG 
GGTGGCGGCC GTTACGGAAT CTTCATCACC AGCAGAGAGC TGCAGGCCTC AGACCCTGAC 
AGCCCCACAG AGGAGCTCGA GTTCTCCATC AGCAGACCTC CACTCTTAGG ATACCTGGAG 
AACGCCGCCA CAGGGTCGTA CATCAAAGGT CGTTTCACGC AGCGTGACGT GGATCGGCGA 
GCCGTGATGT TCGTCCTCCC CGCTGACGTG GAAGTGACGT CTGACAGCTT CCTGTTTGTC 
CTCAGAGATC CGGCAGGAAA CAGCGCGTTG CCTCAGACGC TGGAGCTGTC CTGGTCTCGA 
GTGGAGCTGT CGGCGACCTG CTACAGAACC TGTGAGACGG CCGGGATACT TCAGATCCGC 
GTGCAGCGCA ACGGGAGGAG CGCTGACCCT GCCTACGTCG CCATCCAGGT GGACGAAGGA 
TCAGCCAAAG CTGGCAGAGA TTTCACTCAC AGCACTGCGA GTCTCATCCA GTTCGATCCA 
GGTGTGAAGG TGAAAACCTG GAACATTTAC CTGATTGACG ACGGCCTGGA AGAAAACCAC 
GAGAGCTTCA CGGTCACGCT GAGAAACCCC CAGAACGCCG TCCTGGGACC AAGGAGCTCG 
GCCACCGTGG AGATCATTGA CCCCCACCGA GGAAGCTGTG ACCCAGACGA CCTGTGGGTA 
GAAGATGATC AGCGCCCCCT TCCTCCTCCT CCTACTCCTC CCCCTCTCCC TCCTCAGCTC 
CCTGATCCTC TGCAGCAAGA GGAGGAAGAG GAGGTTCCGG TCATCAATAT AGAGGCGGAG 
CTGCTGTGGG AGAACCAGCC TCACCCGCCC CGAGGAGACG TCCCGAATCG ACATCACCTG 
GACTACAAAG AGGTGGAGCC ACGAGACCCA GAGCCCCACA GCAATGGCTA CACCCCCCAC 
AGCAGCCACC TAAACATTCA GCAGCACACT GGAAGCAGTA CTGGGAACAC TGGGAGCACG 
GTCCGTCTGT CTGCACAGAG GAGGCCTGAG GACGAGCAGG AGAAGGTTTG GACGTTCCAC 
AGTCTGACTC CTCTCCGGCT GGAGGAGCTG CAACCGGGCG ATGTCGAGTG GAGCTGGTCA 
GCTCACGGTC GTCCCCTGCA GGAACTCCAA GTTGGAGATT CTCCTCAGAT GGCTCATCAA 
GCTCAGAAAC ACCAACCCGA GCTCCGCCAG CACAAGGTGG GGAAGTCTGT GAGCAGCTCA 
TGTCCTGATG GCTGGACGCA CCACAGGAGA CGATGTTACA TCCTCAGCCC GTCCGTGGCG 
AGCTGGGCCA GCGCCGACAG AACCTGCTCG CTGCTGTTTA ACAGCAGCCT GACCGACGTG 
CACTCCAGGA GAGATGTGAG CTGGTTGTGG AGGTTTGCAG GGAAGAAGCC ATTTTGGATC 
GGTCTGTCTG GAGGTCCAGG ACGCTCATCG TGGGCTGACA GTCGCTCGCT GTCTTCCTCC 
AGACTGAGGG GGGCGCCAGT GAGCCCCGGT GAGCCCGAGA ACGACTCCAA CTGCGTGCTG 
GTTCAAAACC CGAGAAGCTG GATCTCCACG AGCTGCTCTG CAGAAACTCA GCACTCATTC 
ATCTGTTCAT CGCCAGCTCA AACTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101486624 ( XM_024800597.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_024656365.1 Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), mRNA.

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CloneID	OMa357637
Clone ID Related Accession (Same CDS sequence)	XM_024800597.1
Accession Version	XM_024800597.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1524412800000
Organism	Maylandia zebra(zebra mbuna)
Product	LOW QUALITY PROTEIN: FRAS1-related extracellular matrix protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020192659.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 12 indels internal stop codons :: corrected 1 genomic stop codons ##RefSeq-Attributes-END##

ATGGCTGCCG TCCTCGTGTC CCTGCTGCTG CTCGCGGTGA CGGGCGTTTG CCCTCGGACT 
CTGGTTGTGG TGAACTCTGC CGTGGAGGTC AGCAGAGGGC GCTCGGTTTT CATCACGAGG 
GCGGAGCTTC AGATCAGCGT GGACCCGAGC GCCGACTGTA AGGTGGAGGT GGTGATGAAC 
GAGCCCGTGA CTCAGAGGGT CGGGAGGCTG AGCCCACAGG TGTTTGACTG CAGCTTCCTG 
GAGGACGAGG TGAAGTATGT CCACGACGGC AGCCCTCTGC TGGACGAGGA CGTGGTGATG 
CTCAGAGTCT ACAGGTTCAC GTCCTCGGAC ACGCAGGTGG AGACGGTGAT CCTGAGGGTC 
CGGGTGGTGG ACTCTGGTGC GGGCGTGGTG GCACTCGGCA GCGCTCCGCT GGTCGTTCCC 
CAGTTCTACG GTTTATCCAA CGCCATCAAC GGCTCTGTGC TGAATATCAG GGCCGCGCCT 
GACCTTGTCT GCACCGTCAG GCTGTTGACC GCAGACACCA GCGTCCCCGC TGCGGGTCGG 
CTGGTGAGGG AGGAAGACTC CACGCAGAGG AAGGGTCGAC AGGCGACGCT CTGCCCAGGA 
AACAGTCCGT GTCTTCACGA CACCACCGAG GTCCACTTCC TCAAAACCAG CTGCCATGAA 
TTCCTGAGCT CTGCCCTCAA GTACCAGCAC CTCAGCCCGC CGTCCCCGGA GATCGACTAC 
GTCCCCGTCA GGGTGGAGCT GAGAGAGCAG GCGAGCAGAG CACTGCTGGA GTCACAGGCC 
GTGTGGCTGC CGGTTTTGAT CCACGGGGCC ATGCAGAACC AGCCCCCGCA GGCTACCTTC 
ATGGCCTCTT TCATCCTGGA GGTGGACCAG TTCATCCTCA CCCCGCTCAC CACTGCCACG 
CTGGACGCCA AGGACCACGA GACTCCGCAG GAGAAGCTGG TCTTCAATGT GACCGTCCCA 
CCCGCCGAGG GCTACATCAC ACACCTGGAC GACCACACCA AGCCCATCCG GTCCTTCACA 
TGGCTGGACC TTCACGAGAT GAAAGTGGCC TACCAGCCAT CCAACAGCAG CCGGGGGCGC 
CGCAGGAACT ATGAGGTGGA GTTTCAGGTG ATAGACGGCT CCTATCTGAC CAGCCCACCC 
ATCGTGGTCC ACGTCTCCAT CAGGACATCG GAAACGAATG CTCCGAGAGT CTCCTGGAAC 
GTGGGTCTAG ACCTGTTGGA GGGTCAGTCC CGACCAATCA CCTGGGAGCA GCTGCAGATA 
GTCGACAGCG ACAACATTGA CGCCGTTTAC CTGGTGGCCG TGGACGGACC CCTGCACGGG 
CGGCTCAGTG TCAGAGGCGG GAAGGCGTTT ATGTTCCGCG TGCGGGACCT GAAGGAGGGC 
ACAGTCGTCT ACCACCACTC CGACAGCGAC TCCACCCGCG ACCACGTCGT CTTCCGCATC 
AGCGACGGTC GTCACAGCGT CCGCCACAAG TTCCCCATCA ACATCNCTCC GAAGGACGAC 
TCTCCGCCCT TCCTCATCAA CAACGTGGCG GTGGAGGTGC AGGAGGGTGG GGCCGTGCGG 
CTCGAGGAGT TCATGCTGCT GGCCTCCGAC CTCGACTCCN AGGACGACTA CATCCTTATC 
AGATAGTCTC CGCCCCCGGG CCGGGCACTG GTCAGGAGGA CATCCGCCCA TGAGACAGGG 
TCACCGGTGG CCAGCTTCCT GCAGAGGGAC CTGATCCAGG GTCAGATCTT CTACCAGCAC 
TCGGGAGATG AGCAGTTTGA GGATGCCTTC GACTTCACGC TGTCTGACAG CCACCGGCCG 
CCCAACCTGT CGCAGACATA CACGGTGGTG GTGCACGTGT TCCCGGTGGA GGACCAGGTG 
CCAGTCGAAG TTCCCGGCAG CGTCCGCTCG CTGACAGTGA AGGAGACGGA GGTGGTTTAC 
ATCACCCAGG CTCACCTTCA CTTCACAGAC CGAGAGCACC CAGATGCTGA CCTCACCTAT 
GTCATTACAC AGCCCTGCTT CAGCCCGCTA CATCCTGGCC TGATGGATGC AGGACGTCTG 
TTTTACACCG ACAGCACCAA CGCCATGAAG AAGGACACAN CAGCGCCCGT GCTGAAGTCC 
TTCACTCAGG TTGGCATGGG TTGGGCTTTT GCAGGTTCAG ATGTAATTTC TTGTTTTCTC 
CTANNGCATG CTGTGAACCA CATGAAGGTG GCCTTCATGC CGCGGTCGAG GACATTGGGC 
NNCGAGTCGC TCTTCGTCCA GTTCGTCTTC TCAGTCAGCG ATCACCACGG CGACACCGTC 
TCCGGCCTCA TCTTCAACAT CACGGTTACC CCTGTCGATG ACCAGGCGCC AGAGGCTTTC 
ACCAACCTGC TTCGGGTGGA GGAGGGCGGC GCGGCCTTCG TGACGGAGGA GCACCTCTTG 
GTCCGGGACN GGGACAGTCG AGAGGAGGCG CTGAGGGTGG AGATTCGGAG CACGGCTCGT 
CACGGCCGGC TGGAGCTGTG GGGCCAAGCG CTGCAGCAGG GAGGCGGGTT TACCCTGCAG 
GAACTGAGAG GGCTGGGCAT CAGGTACATC CACGATGACT CTGAGACCAC TGAGGATGTC 
GTTGGTCTCA AGGTGTCGGA CGGTCTGAAC TCTGTGGACG TCGTCCTGCA CGTTCAGGTG 
CTGCCGATGA ACGACGAGCC TCCTCAGCTG GGCGCCGTCT GCAAGGGGNN GCTGAGCTGC 
GAGGAAGGGG GGCGTGTCCA GGTGACGGTG GACTACCTGT CGGCCACCGA CCGGGACAGC 
GACGATTCCG CGCTGNACTA CATGCTCGCC CGGAGACCGA GCAGGGGAGA GCTGCAGCGA 
GCCGGCGTGA CTGTGGACAA GTTCTCCAGC NAGGACGTCC TGCAGGGTCA CATCTACTAT 
GCCCACACAG GCGGGGAGAT CGGACCTGAG CCGGTGTTCG ACACGGTCAC CCTCATCATC 
TCCGACAGTG AGGCCAGAGG GCCGGACAGC TGCTGCNCAC GGGAGGCCCC GCCCCCTCCT 
GTTCCTCTCC ATGGTACACT TCCTGTTTAC GATCTGAACA TCACAGTTCT TCCTGTCAAC 
GATAAAGTCC CTGCTGTCAC TCTGGGCGAG TCCATGCTGG TGGTGGATGA AGGCTCTTCT 
ATCTGTCTGT GCGGGGGGGT CCTCGGAGCG TCAGACCCAG ACAGTCCCCC TGAGCAGCTC 
GTCTTTCACC TGGAGACTCC TCCCNTTCAC GGCTTCCTGG AGAACACGCA GCCGATGCCC 
GGCTCCGAAA AGAGCAACGC GGGAGTGCGA GTCGAATCCT TCACTCTCAA ACACCTGACC 
TCTGGCTTCA TCAACTACGT GCAATCGGAG CACAAAGGGG TGGAGCCAAC TGTCGACCAG 
CTGTCCATCA GTGTGAGCGA CGGGCTACAT CGCTCCGCCC CCGTCTCCTT CTACATCATT 
ATCAAACCAA CCAATGACGA AACACCATCG CTGCTGCTCG CCAACTTCAC CGTAAAAGAG 
GGCGGGATGA GGGAGCTAAT TCCATCCATA CTGGATGGTT TTGACTTGGA CGCCCCACCT 
GAGCTGCTCA CCTTCACTGT GGTGCAGCCG CCGGCTCACG GCAGCCTGAT TAACAGCATC 
CACGGCACGG AGGCGGGAGC TGACCTCCTG CACAGGAGCC TCTCTGTCAC CTCTTTCACT 
CTGCAGGAGC TCCGACAAGG CATGAGGATC GTCTACATGC ACGATGACAC AGAAACCCTG 
AAGGACGCCG TGGCTCTGCG GCTGACAGAT GGTGTCCATG CAGTTGAGGG AACAGTTCAG 
GTCACCGTGC TGCTGGTCAA CGACGAGAAA CCACGACTGC TCAAGAATGC TGGACTGCAG 
GTGGACTCAG GTGAGCTCAG GGTGATCTCC AGTGTGGTTC TGGAGGCTGA GGACCTGGAC 
ACGCCCCCAG AACAGGTGTA CTACTTCCTT AATGTTGCTC CGCGGTTTGG AAAACTGCAG 
CTGAAGACGA CGTCGGGGTG GAACGAGCTG ACGGCGGGTC AGAACTTCAC ACAGGGCGAC 
GTGGAGATGA ACCGACTGTG GTACCAACAC ACCGCCGCCA CCAACGCAGC CGGCTTTAAA 
GGTCACGACA GCTTCCGCTT CACCCTCAGC GACCTGGACA ACGAGTCTCC GGCTCAGAGC 
TTCTTCATCT CTGTGCACGT GATGCAGAAA GGGGACATCG TGGTGCTGAG CAGGCCAGTG 
CACCTGAGGG AGGGGCAGCG TGTCGTCCTG AACACTGACA TCCTGCTGGC ATCAGACTCG 
ACTGGCCGGC CCAAGGAGCT GGTCTACACG GTGTCTGACC CGCCGCGTCA CGGCCTCGTC 
CACGCCGTCC ACCGGCCTGG AGTCCCGCTA CTCAGCTTCA CCCAGCTGGA CGTGGCTGCG 
CACCGAGTCT GCTACACCCA CGACAACAGC CATGATGCCG AGGCTGACTC CTTCAGTTTT 
GTTGTTACAA ACGGTGACTC GTCTCGCAGC TCCACCCTCC ACTTCACCAT CGAGCATGGT 
GACCGCATCC CGCCCACCCT CAGCCGCAAC GTTGGCCTCC GGCTGCAGGA TGGCTCCACA 
GTGACCATCA CTTCGGACCA GCTGCAGCTC ACCGACCCCG ACACTACCAC CGCCAACCTG 
GGATACGTCA TCACGCAGCT GCCGCAGCAC GGGAAGCTGC TGCTGAGAGG CACGCCGCTA 
CCCTCGCCGC CCCGGTTCTC CCAGACGAAC GTGGACGACC TCCACCTGAC TTACCAACAC 
GACCCGAGCA GCCCTGCGAA GATCGACNGG TTCTACTTTA TGCCGAGCGA CGGACACAAC 
AGGGGCTACC TGGAGTTTGG GCAGCTGAGG GAGGAGCCTG CTGTGTTTGA CATACAGGTG 
GATTGGGTCG ACCGGACGCC TCCCACTCTG ACCACCAGGG GGAGTCCCAG CACCGTGGTG 
GATCTGGGCG GCGGCCGTTA CGGAATCTTC ATCACCAGCA GAGAGCTGCA GGCCTCAGAC 
CCTGACAGCC CCACAGAGGA GCTCGAGTTC TCCATCAGCA GACCTCCACT CTTAGGATAC 
CTGGAGAACG CCGCCACAGG GTCGTACATC AAAGGTCGTT TCACGCAGCG TGACGTGGAT 
CGGCGAGCCG TGATGTTCGT CCTCCCCGCT GACGTGGAAG TGACGTCTGA CAGCTTCCTG 
TTTGTCCTCA GAGATCCGGC AGGAAACAGC GCGTTGCCTC AGACGCTGGA GCTGTCCTGG 
TCTCGAGTGG AGCTGTCGGC GACCTGCTAC AGAACCTGTG AGACGGCCGG GATACTTCAG 
ATCCGCGTGC AGCGCAACGG GAGGAGCGCT GACCCTGCCT ACGTCGCCAT CCAGGTGGAC 
GAAGGATCAG CCAAAGCTGG CAGAGATTTC ACTCACAGCA CTGCGAGTCT CATCCAGTTC 
GATCCAGGTG TGAAGGTGAA AACCTGGAAC ATTTACCTGA TTGACGACGG CCTGGAAGAA 
AACCACGAGA GCTTCACGGT CACGCTGAGA AACCCCCAGA ACGCCGTCCT GGGACCAAGG 
AGCTCGGCCA CCGTGGAGAT CATCGACCCC CACCGAGGAA GCTGTGACCC AGACGACCTG 
TGGGTAGAAG ATGATCAGCG CCCCCTTCCT CCTCCTCCTA CTCCTCCCCC TCTCCCTCCT 
CAGCTCCCTG ATCCTCTGCA GCAAGAGGAG GAAGAGGAGG TTCCGGTCAT CAATATAGAG 
GCGGAGCTGC TGTGGGAGAA CCAGCCTCAC CCGCCCCGAG GAGACGTCCC GAATCGACAT 
CACCTGGACT ACAAAGAGGT GGAGCCACGA GACCCAGAGC CCCACAGCAA TGGCTACACC 
CCCCACAGCA GCCACCTAAA CATTCAGCAG CACACTGGAA GCAGTACTGG GAACACTGGG 
AGCACGGTCC GTCTGTCTGC ACAGAGGAGG CCTGAGGACG AGCAGGAGAA GGTTTGGACG 
TTCCACAGTC TGACTCCTCT CCGGCTGGAG GAGCTGCAAC CGGGCGATGT CGAGTGGAGC 
TGGTCAGCTC ACGGTCGTCC CCTGCAGGAA CTCCAAGTTG GAGATTCTCC TCAGATGGCT 
CATCAAGCTC AGAAACACCA ACCCGAGCTC CGCCAGCACA AGGTGGGGAA GTCTGTGAGC 
AGCTCATGTC CTGATGGCTG GACGCACCAC AGGAGACGAT GTTACATCCT CAGCCCGTCC 
GTGGCGAGCT GGGCCAGCGC CGACAGAACC TGCTCGCTGC TGTTTAACAG CAGCCTGACC 
GACGTGCACT CCAGGAGAGA TGTGAGCTGG TTGTGGAGGT TTGCAGGGAA GAAGCCATTT 
TGGATCGGTC TGTCTGGAGG TCCAGGACGC TCATCGTGGG CTGACAGTCG CTCGCTGTCT 
TCCTCCAGAC TGAGGGGGGC GCCAGTGAGC CCCGGTGAGC CCGAGAACGA CTCCAACTGC 
GTGCTGGTTC AAAACCCGAG AAGCTGGATC TCCACGAGCT GCTCTGCAGA AACTCAGCAC 
TCATTCATCT GTTCATCGCC AGCTCAAACT CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024656365.1
CDS	303..6818
Translation	MAAVLVSLLLLAVTGVCPRTLVVVNSAVEVSRGRSVFITRAELQISVDPSADCKVEVVMNEPVTQRVGRLSPQVFDCSFLEDEVKYVHDGSPLLDEDVVMLRVYRFTSSDTQVETVILRVRVVDSGAGVVALGSAPLVVPQFYGLSNAINGSVLNIRAAPDLVCTVRLLTADTSVPAAGRLVREEDSTQRKGRQATLCPGNSPCLHDTTEVHFLKTSCHEFLSSALKYQHLSPPSPEIDYVPVRVELREQASRALLESQAVWLPVLIHGAMQNQPPQATFMASFILEVDQFILTPLTTATLDAKDHETPQEKLVFNVTVPPAEGYITHLDDHTKPIRSFTWLDLHEMKVAYQPSNSSRGRRRNYEVEFQVIDGSYLTSPPIVVHVSIRTSETNAPRVSWNVGLDLLEGQSRPITWEQLQIVDSDNIDAVYLVAVDGPLHGRLSVRGGKAFMFRVRDLKEGTVVYHHSDSDSTRDHVVFRISDGRHSVRHKFPINInullPKDDSPPFLINNVAVEVQEGGAVRLEEFMLLASDLDSnullDDYILIR*SPPPGRALVRRTSAHETGSPVASFLQRDLIQGQIFYQHSGDEQFEDAFDFTLSDSHRPPNLSQTYTVVVHVFPVEDQVPVEVPGSVRSLTVKETEVVYITQAHLHFTDREHPDADLTYVITQPCFSPLHPGLMDAGRLFYTDSTNAMKKDTnullAPVLKSFTQVGMGWAFAGSDVISCFLLnullHAVNHMKVAFMPRSRTLGnullESLFVQFVFSVSDHHGDTVSGLIFNITVTPVDDQAPEAFTNLLRVEEGGAAFVTEEHLLVRDnullDSREEALRVEIRSTARHGRLELWGQALQQGGGFTLQELRGLGIRYIHDDSETTEDVVGLKVSDGLNSVDVVLHVQVLPMNDEPPQLGAVCKGnullLSCEEGGRVQVTVDYLSATDRDSDDSALnullYMLARRPSRGELQRAGVTVDKFSSnullDVLQGHIYYAHTGGEIGPEPVFDTVTLIISDSEARGPDSCCnullREAPPPPVPLHGTLPVYDLNITVLPVNDKVPAVTLGESMLVVDEGSSICLCGGVLGASDPDSPPEQLVFHLETPPnullHGFLENTQPMPGSEKSNAGVRVESFTLKHLTSGFINYVQSEHKGVEPTVDQLSISVSDGLHRSAPVSFYIIIKPTNDETPSLLLANFTVKEGGMRELIPSILDGFDLDAPPELLTFTVVQPPAHGSLINSIHGTEAGADLLHRSLSVTSFTLQELRQGMRIVYMHDDTETLKDAVALRLTDGVHAVEGTVQVTVLLVNDEKPRLLKNAGLQVDSGELRVISSVVLEAEDLDTPPEQVYYFLNVAPRFGKLQLKTTSGWNELTAGQNFTQGDVEMNRLWYQHTAATNAAGFKGHDSFRFTLSDLDNESPAQSFFISVHVMQKGDIVVLSRPVHLREGQRVVLNTDILLASDSTGRPKELVYTVSDPPRHGLVHAVHRPGVPLLSFTQLDVAAHRVCYTHDNSHDAEADSFSFVVTNGDSSRSSTLHFTIEHGDRIPPTLSRNVGLRLQDGSTVTITSDQLQLTDPDTTTANLGYVITQLPQHGKLLLRGTPLPSPPRFSQTNVDDLHLTYQHDPSSPAKIDnullFYFMPSDGHNRGYLEFGQLREEPAVFDIQVDWVDRTPPTLTTRGSPSTVVDLGGGRYGIFITSRELQASDPDSPTEELEFSISRPPLLGYLENAATGSYIKGRFTQRDVDRRAVMFVLPADVEVTSDSFLFVLRDPAGNSALPQTLELSWSRVELSATCYRTCETAGILQIRVQRNGRSADPAYVAIQVDEGSAKAGRDFTHSTASLIQFDPGVKVKTWNIYLIDDGLEENHESFTVTLRNPQNAVLGPRSSATVEIIDPHRGSCDPDDLWVEDDQRPLPPPPTPPPLPPQLPDPLQQEEEEEVPVINIEAELLWENQPHPPRGDVPNRHHLDYKEVEPRDPEPHSNGYTPHSSHLNIQQHTGSSTGNTGSTVRLSAQRRPEDEQEKVWTFHSLTPLRLEELQPGDVEWSWSAHGRPLQELQVGDSPQMAHQAQKHQPELRQHKVGKSVSSSCPDGWTHHRRRCYILSPSVASWASADRTCSLLFNSSLTDVHSRRDVSWLWRFAGKKPFWIGLSGGPGRSSWADSRSLSSSRLRGAPVSPGEPENDSNCVLVQNPRSWISTSCSAETQHSFICSSPAQTH

Target ORF information:

RefSeq Version	XM_024800597.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra FRAS1-related extracellular matrix protein 1 (LOC101486624), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024800597.1

ORF Insert Sequence:

ATGGCTGCCG TCCTCGTGTC CCTGCTGCTG CTCGCGGTGA CGGGCGTTTG CCCTCGGACT 
CTGGTTGTGG TGAACTCTGC CGTGGAGGTC AGCAGAGGGC GCTCGGTTTT CATCACGAGG 
GCGGAGCTTC AGATCAGCGT GGACCCGAGC GCCGACTGTA AGGTGGAGGT GGTGATGAAC 
GAGCCCGTGA CTCAGAGGGT CGGGAGGCTG AGCCCACAGG TGTTTGACTG CAGCTTCCTG 
GAGGACGAGG TGAAGTATGT CCACGACGGC AGCCCTCTGC TGGACGAGGA CGTGGTGATG 
CTCAGAGTCT ACAGGTTCAC GTCCTCGGAC ACGCAGGTGG AGACGGTGAT CCTGAGGGTC 
CGGGTGGTGG ACTCTGGTGC GGGCGTGGTG GCACTCGGCA GCGCTCCGCT GGTCGTTCCC 
CAGTTCTACG GTTTATCCAA CGCCATCAAC GGCTCTGTGC TGAATATCAG GGCCGCGCCT 
GACCTTGTCT GCACCGTCAG GCTGTTGACC GCAGACACCA GCGTCCCCGC TGCGGGTCGG 
CTGGTGAGGG AGGAAGACTC CACGCAGAGG AAGGGTCGAC AGGCGACGCT CTGCCCAGGA 
AACAGTCCGT GTCTTCACGA CACCACCGAG GTCCACTTCC TCAAAACCAG CTGCCATGAA 
TTCCTGAGCT CTGCCCTCAA GTACCAGCAC CTCAGCCCGC CGTCCCCGGA GATCGACTAC 
GTCCCCGTCA GGGTGGAGCT GAGAGAGCAG GCGAGCAGAG CACTGCTGGA GTCACAGGCC 
GTGTGGCTGC CGGTTTTGAT CCACGGGGCC ATGCAGAACC AGCCCCCGCA GGCTACCTTC 
ATGGCCTCTT TCATCCTGGA GGTGGACCAG TTCATCCTCA CCCCGCTCAC CACTGCCACG 
CTGGACGCCA AGGACCACGA GACTCCGCAG GAGAAGCTGG TCTTCAATGT GACCGTCCCA 
CCCGCCGAGG GCTACATCAC ACACCTGGAC GACCACACCA AGCCCATCCG GTCCTTCACA 
TGGCTGGACC TTCACGAGAT GAAAGTGGCC TACCAGCCAT CCAACAGCAG CCGGGGGCGC 
CGCAGGAACT ATGAGGTGGA GTTTCAGGTG ATAGACGGCT CCTATCTGAC CAGCCCACCC 
ATCGTGGTCC ACGTCTCCAT CAGGACATCG GAAACGAATG CTCCGAGAGT CTCCTGGAAC 
GTGGGTCTAG ACCTGTTGGA GGGTCAGTCC CGACCAATCA CCTGGGAGCA GCTGCAGATA 
GTCGACAGCG ACAACATTGA CGCCGTTTAC CTGGTGGCCG TGGACGGACC CCTGCACGGG 
CGGCTCAGTG TCAGAGGCGG GAAGGCGTTT ATGTTCCGCG TGCGGGACCT GAAGGAGGGC 
ACAGTCGTCT ACCACCACTC CGACAGCGAC TCCACCCGCG ACCACGTCGT CTTCCGCATC 
AGCGACGGTC GTCACAGCGT CCGCCACAAG TTCCCCATCA ACATCNCTCC GAAGGACGAC 
TCTCCGCCCT TCCTCATCAA CAACGTGGCG GTGGAGGTGC AGGAGGGTGG GGCCGTGCGG 
CTCGAGGAGT TCATGCTGCT GGCCTCCGAC CTCGACTCCN AGGACGACTA CATCCTTATC 
AGATAGTCTC CGCCCCCGGG CCGGGCACTG GTCAGGAGGA CATCCGCCCA TGAGACAGGG 
TCACCGGTGG CCAGCTTCCT GCAGAGGGAC CTGATCCAGG GTCAGATCTT CTACCAGCAC 
TCGGGAGATG AGCAGTTTGA GGATGCCTTC GACTTCACGC TGTCTGACAG CCACCGGCCG 
CCCAACCTGT CGCAGACATA CACGGTGGTG GTGCACGTGT TCCCGGTGGA GGACCAGGTG 
CCAGTCGAAG TTCCCGGCAG CGTCCGCTCG CTGACAGTGA AGGAGACGGA GGTGGTTTAC 
ATCACCCAGG CTCACCTTCA CTTCACAGAC CGAGAGCACC CAGATGCTGA CCTCACCTAT 
GTCATTACAC AGCCCTGCTT CAGCCCGCTA CATCCTGGCC TGATGGATGC AGGACGTCTG 
TTTTACACCG ACAGCACCAA CGCCATGAAG AAGGACACAN CAGCGCCCGT GCTGAAGTCC 
TTCACTCAGG TTGGCATGGG TTGGGCTTTT GCAGGTTCAG ATGTAATTTC TTGTTTTCTC 
CTANNGCATG CTGTGAACCA CATGAAGGTG GCCTTCATGC CGCGGTCGAG GACATTGGGC 
NNCGAGTCGC TCTTCGTCCA GTTCGTCTTC TCAGTCAGCG ATCACCACGG CGACACCGTC 
TCCGGCCTCA TCTTCAACAT CACGGTTACC CCTGTCGATG ACCAGGCGCC AGAGGCTTTC 
ACCAACCTGC TTCGGGTGGA GGAGGGCGGC GCGGCCTTCG TGACGGAGGA GCACCTCTTG 
GTCCGGGACN GGGACAGTCG AGAGGAGGCG CTGAGGGTGG AGATTCGGAG CACGGCTCGT 
CACGGCCGGC TGGAGCTGTG GGGCCAAGCG CTGCAGCAGG GAGGCGGGTT TACCCTGCAG 
GAACTGAGAG GGCTGGGCAT CAGGTACATC CACGATGACT CTGAGACCAC TGAGGATGTC 
GTTGGTCTCA AGGTGTCGGA CGGTCTGAAC TCTGTGGACG TCGTCCTGCA CGTTCAGGTG 
CTGCCGATGA ACGACGAGCC TCCTCAGCTG GGCGCCGTCT GCAAGGGGNN GCTGAGCTGC 
GAGGAAGGGG GGCGTGTCCA GGTGACGGTG GACTACCTGT CGGCCACCGA CCGGGACAGC 
GACGATTCCG CGCTGNACTA CATGCTCGCC CGGAGACCGA GCAGGGGAGA GCTGCAGCGA 
GCCGGCGTGA CTGTGGACAA GTTCTCCAGC NAGGACGTCC TGCAGGGTCA CATCTACTAT 
GCCCACACAG GCGGGGAGAT CGGACCTGAG CCGGTGTTCG ACACGGTCAC CCTCATCATC 
TCCGACAGTG AGGCCAGAGG GCCGGACAGC TGCTGCNCAC GGGAGGCCCC GCCCCCTCCT 
GTTCCTCTCC ATGGTACACT TCCTGTTTAC GATCTGAACA TCACAGTTCT TCCTGTCAAC 
GATAAAGTCC CTGCTGTCAC TCTGGGCGAG TCCATGCTGG TGGTGGATGA AGGCTCTTCT 
ATCTGTCTGT GCGGGGGGGT CCTCGGAGCG TCAGACCCAG ACAGTCCCCC TGAGCAGCTC 
GTCTTTCACC TGGAGACTCC TCCCNTTCAC GGCTTCCTGG AGAACACGCA GCCGATGCCC 
GGCTCCGAAA AGAGCAACGC GGGAGTGCGA GTCGAATCCT TCACTCTCAA ACACCTGACC 
TCTGGCTTCA TCAACTACGT GCAATCGGAG CACAAAGGGG TGGAGCCAAC TGTCGACCAG 
CTGTCCATCA GTGTGAGCGA CGGGCTACAT CGCTCCGCCC CCGTCTCCTT CTACATCATT 
ATCAAACCAA CCAATGACGA AACACCATCG CTGCTGCTCG CCAACTTCAC CGTAAAAGAG 
GGCGGGATGA GGGAGCTAAT TCCATCCATA CTGGATGGTT TTGACTTGGA CGCCCCACCT 
GAGCTGCTCA CCTTCACTGT GGTGCAGCCG CCGGCTCACG GCAGCCTGAT TAACAGCATC 
CACGGCACGG AGGCGGGAGC TGACCTCCTG CACAGGAGCC TCTCTGTCAC CTCTTTCACT 
CTGCAGGAGC TCCGACAAGG CATGAGGATC GTCTACATGC ACGATGACAC AGAAACCCTG 
AAGGACGCCG TGGCTCTGCG GCTGACAGAT GGTGTCCATG CAGTTGAGGG AACAGTTCAG 
GTCACCGTGC TGCTGGTCAA CGACGAGAAA CCACGACTGC TCAAGAATGC TGGACTGCAG 
GTGGACTCAG GTGAGCTCAG GGTGATCTCC AGTGTGGTTC TGGAGGCTGA GGACCTGGAC 
ACGCCCCCAG AACAGGTGTA CTACTTCCTT AATGTTGCTC CGCGGTTTGG AAAACTGCAG 
CTGAAGACGA CGTCGGGGTG GAACGAGCTG ACGGCGGGTC AGAACTTCAC ACAGGGCGAC 
GTGGAGATGA ACCGACTGTG GTACCAACAC ACCGCCGCCA CCAACGCAGC CGGCTTTAAA 
GGTCACGACA GCTTCCGCTT CACCCTCAGC GACCTGGACA ACGAGTCTCC GGCTCAGAGC 
TTCTTCATCT CTGTGCACGT GATGCAGAAA GGGGACATCG TGGTGCTGAG CAGGCCAGTG 
CACCTGAGGG AGGGGCAGCG TGTCGTCCTG AACACTGACA TCCTGCTGGC ATCAGACTCG 
ACTGGCCGGC CCAAGGAGCT GGTCTACACG GTGTCTGACC CGCCGCGTCA CGGCCTCGTC 
CACGCCGTCC ACCGGCCTGG AGTCCCGCTA CTCAGCTTCA CCCAGCTGGA CGTGGCTGCG 
CACCGAGTCT GCTACACCCA CGACAACAGC CATGATGCCG AGGCTGACTC CTTCAGTTTT 
GTTGTTACAA ACGGTGACTC GTCTCGCAGC TCCACCCTCC ACTTCACCAT CGAGCATGGT 
GACCGCATCC CGCCCACCCT CAGCCGCAAC GTTGGCCTCC GGCTGCAGGA TGGCTCCACA 
GTGACCATCA CTTCGGACCA GCTGCAGCTC ACCGACCCCG ACACTACCAC CGCCAACCTG 
GGATACGTCA TCACGCAGCT GCCGCAGCAC GGGAAGCTGC TGCTGAGAGG CACGCCGCTA 
CCCTCGCCGC CCCGGTTCTC CCAGACGAAC GTGGACGACC TCCACCTGAC TTACCAACAC 
GACCCGAGCA GCCCTGCGAA GATCGACNGG TTCTACTTTA TGCCGAGCGA CGGACACAAC 
AGGGGCTACC TGGAGTTTGG GCAGCTGAGG GAGGAGCCTG CTGTGTTTGA CATACAGGTG 
GATTGGGTCG ACCGGACGCC TCCCACTCTG ACCACCAGGG GGAGTCCCAG CACCGTGGTG 
GATCTGGGCG GCGGCCGTTA CGGAATCTTC ATCACCAGCA GAGAGCTGCA GGCCTCAGAC 
CCTGACAGCC CCACAGAGGA GCTCGAGTTC TCCATCAGCA GACCTCCACT CTTAGGATAC 
CTGGAGAACG CCGCCACAGG GTCGTACATC AAAGGTCGTT TCACGCAGCG TGACGTGGAT 
CGGCGAGCCG TGATGTTCGT CCTCCCCGCT GACGTGGAAG TGACGTCTGA CAGCTTCCTG 
TTTGTCCTCA GAGATCCGGC AGGAAACAGC GCGTTGCCTC AGACGCTGGA GCTGTCCTGG 
TCTCGAGTGG AGCTGTCGGC GACCTGCTAC AGAACCTGTG AGACGGCCGG GATACTTCAG 
ATCCGCGTGC AGCGCAACGG GAGGAGCGCT GACCCTGCCT ACGTCGCCAT CCAGGTGGAC 
GAAGGATCAG CCAAAGCTGG CAGAGATTTC ACTCACAGCA CTGCGAGTCT CATCCAGTTC 
GATCCAGGTG TGAAGGTGAA AACCTGGAAC ATTTACCTGA TTGACGACGG CCTGGAAGAA 
AACCACGAGA GCTTCACGGT CACGCTGAGA AACCCCCAGA ACGCCGTCCT GGGACCAAGG 
AGCTCGGCCA CCGTGGAGAT CATCGACCCC CACCGAGGAA GCTGTGACCC AGACGACCTG 
TGGGTAGAAG ATGATCAGCG CCCCCTTCCT CCTCCTCCTA CTCCTCCCCC TCTCCCTCCT 
CAGCTCCCTG ATCCTCTGCA GCAAGAGGAG GAAGAGGAGG TTCCGGTCAT CAATATAGAG 
GCGGAGCTGC TGTGGGAGAA CCAGCCTCAC CCGCCCCGAG GAGACGTCCC GAATCGACAT 
CACCTGGACT ACAAAGAGGT GGAGCCACGA GACCCAGAGC CCCACAGCAA TGGCTACACC 
CCCCACAGCA GCCACCTAAA CATTCAGCAG CACACTGGAA GCAGTACTGG GAACACTGGG 
AGCACGGTCC GTCTGTCTGC ACAGAGGAGG CCTGAGGACG AGCAGGAGAA GGTTTGGACG 
TTCCACAGTC TGACTCCTCT CCGGCTGGAG GAGCTGCAAC CGGGCGATGT CGAGTGGAGC 
TGGTCAGCTC ACGGTCGTCC CCTGCAGGAA CTCCAAGTTG GAGATTCTCC TCAGATGGCT 
CATCAAGCTC AGAAACACCA ACCCGAGCTC CGCCAGCACA AGGTGGGGAA GTCTGTGAGC 
AGCTCATGTC CTGATGGCTG GACGCACCAC AGGAGACGAT GTTACATCCT CAGCCCGTCC 
GTGGCGAGCT GGGCCAGCGC CGACAGAACC TGCTCGCTGC TGTTTAACAG CAGCCTGACC 
GACGTGCACT CCAGGAGAGA TGTGAGCTGG TTGTGGAGGT TTGCAGGGAA GAAGCCATTT 
TGGATCGGTC TGTCTGGAGG TCCAGGACGC TCATCGTGGG CTGACAGTCG CTCGCTGTCT 
TCCTCCAGAC TGAGGGGGGC GCCAGTGAGC CCCGGTGAGC CCGAGAACGA CTCCAACTGC 
GTGCTGGTTC AAAACCCGAG AAGCTGGATC TCCACGAGCT GCTCTGCAGA AACTCAGCAC 
TCATTCATCT GTTCATCGCC AGCTCAAACT CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC101486624 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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