col4a5 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	col4a5
Entrez Gene ID	101472438
Full Name	collagen type IV alpha 5 chain
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014413308.1	XP_014268794.1	collagen alpha-5(IV) chain isoform X1
XM_014413309.1	XP_014268795.1	collagen alpha-5(IV) chain isoform X2
XM_024801203.1	XP_024656971.1	collagen alpha-5(IV) chain

Pathways from BioSystems
Gene Ontology

The following col4a5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col4a5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMa24137	XM_014413308.1 Latest version!	Maylandia zebra collagen type IV alpha 5 chain (col4a5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMa24138	XM_014413309.1 Latest version!	Maylandia zebra collagen type IV alpha 5 chain (col4a5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

col4a5 ( XM_014413308.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014268794.1 Maylandia zebra collagen type IV alpha 5 chain (col4a5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa24137
Clone ID Related Accession (Same CDS sequence)	XM_014413308.1
Accession Version	XM_014413308.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	collagen alpha-5(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447357.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAG ACTCAAAGTG CCTGCGACAG CTCGGTGCGA CTCTGCTACT AGTCCTGTTC 
GCCGGGGTGC AGCGATCCGA CTCTGCGGCT TGCCATGGAT GTGCAGGATC CAAATGTGAC 
TGCAGTGGAG TGAAAGGAGA AAAGGGTGAA AGGGGTTTCC CAGGTTTGAC AGGACAGCCA 
GGCGTCCCAG GATTCCCTGG ACCAGAAGGA CCAATTGGGC CCAGAGGAGA GAAGGGAAGT 
GATGGACCTC AAGGACCAGG TGGTCCAAAA GGAATTAGAG GACCTCCAGG AACACCAGGA 
TTTCCAGGAA CACCCGGACT CCCAGGTCTA CCTGGGCAGG ATGGTCCTCC AGGTCCAAGA 
GGAGTGCCGG GTTGCAATGG GACAAAGGGT GACAGGGGTT TCCCAGGAAG TCCAGGAATC 
CCTGGGCAGC CAGGACTTCA AGGTCCACCT GGTCTGCCAG GACAAAAGGG AGACCCAGGT 
GATGTTATCT CCGCCAATCG TTTCGGAGAG AAAGGAGCAG TGGGTTTACC CGGTTTGCCA 
GGAGGTCCTG GCCGACCTGG ACCTCCAGGT ATACAAGGTC CGGTAGGTCC CCCAGGACCC 
AGAGGCCTTG AGGGTCGTCC AGGTGCTCCT GGTCCACCCG GGCCTAAGGG GAATATGGGT 
TTGAATTTCC AAGGACCCAA AGGAGAAAAG GGTGCACCAG GTTTGCAAGG ACCTCCTGGT 
CCTCCAGGAC AGGTTGGGGA GCAGACAAAG CCGGCTGAGA CTGAAATTCA GAGAGGAGAC 
AAGGGTGACC AAGGACCTCC TGGCCCTAAA GGTGATCCGG GTTTACCGGG ACCTCCTGGT 
CCCTATGGTG GGCAGAAAGG AGAAAAGGGA GAACCAGGAG AGCTGGGAAA ACGAGGAAAA 
CCAGGCAAAG ATGGTGATCC TGGTTCTCCA GGATACCCAG GGCCCCCTGG AGAGACAGGC 
CAGCCTGGTC TTCCAGGGAG GGATGGTGAG ACAGGACAAA AAGGTGATCG TGGATTCCCG 
GGTCCACCAG GGCAGGTGAT TGGCGGGCCA ATAGGGAATC AGGTGGGGCC TAAAGGTGAA 
CCTGGCTTAC CTGGAACACC TGGACTCAAA GGAGACAGAG GAGTACAAGG TTTGACAGGC 
CCTCCAGGAG AACCGGGATT CACAGGAACC GGTAGTGGGG GTTTTCCTGG ACCTCAAGGT 
TTCCCAGGAG AGCGAGGTCA GAAGGGAGAG ATTGGCCCTC CAGGAAACTT TCTGCCAGGC 
CCCCCAGGTC GTGCAGGGTC TCCTGGATCC CCTGGTCCGC CAGGGCCCCG TGGACTTCCA 
GGCACTTCCT CAGGTCAAGA CTGCATACCA GGACTTCCTG GGGTTCCTGG TGCACAAGGA 
GGAAGAGGTT TCCCTGGAGA GATAGGACAG AAAGGTGAGA AAGGTGACAC CTGTGTGAAC 
TGCCTAGGAA GCGTCAATCC CATCCCTGGA CCTCAAGGAC CTCCAGGACC TCCTGGATTT 
CCTGGACCTC CTGGCGTCCA AGGTTTCAAG GGAGACAGAG GTTTTCCAGG AACACCTGGC 
TCTGTCGGCC CCAGCGGTCC TCCTGGTGCT CAAGGTCCTC CCGGCCTAAA TGGAGAGAAG 
GGAGATCCAG GTGATCCCAT CAGAGTTAAT GGTTTGAGTG GAGAGAAGGG TGACACTGGT 
TTCCCTGGAC CACCTGGTCT GCCCGGTCTG GACGGGGCGC CTGGGCCTCC AGGGCCTAAA 
GGAGCTCCTG TTTTGTTGGT GAGGGGAGAA AGAGGCCTCC CCGGTGAACC AGGTTTCCCT 
GGTAATCCAG GAGACAGGGG TCCCCCAGGT CTTCCTGGCT TTGGAAATCC AGGACCTCGT 
GAGAAAGGTG TCCAGGGTGT CTCAGGAAGA CCGGGACCTG TTGGTGCACC AGGAACTAAG 
GGTGAACCTG GTCAGACAAT GACAGAGAAA GGGGCCAAAG GAGAAAGAGG TCGGGATGGC 
GAGCCTGGGC AGCCTGGTTC ACCAGGTATA CCAGGTCAGC CTGGACAGCC TGGTTTTCCC 
GGTTTATCGG GAGCGAAGGG TGAACCCGGA ATCCCAGGCA TTGGACTGCC AGGACCTCCA 
GGACCAAAAG GATTCCCGGG TGTTGGCGGG CAACCAGGAG CCCCTGGAGG ACCAGGAAGA 
CCAGGGATAG ATGGACGTCC CGGTGAGCTG GGATTTCCTG GACCAAAGGG TGAACCTGGT 
CTTGGACTTC CTGGTCCTCC TGGTTTAACG GGTGCACCGG GCCCTAAAGG TTCCATTGGG 
CCTAAGGGAG ATCCTGGTTT CCCTGGTAAC CCCGGTTTGT CAGGACGGCT TGGAGCTGAT 
GGAGCTCCAG GATTCAAAGG TGAGCCTGGT ACACCTGGTA CACCTGGATC TCGTGGCCCC 
CCTGGACCCC CCTCTTTTGG TGGAACGGGC CCACCAGGTC TCCCAGGACC TCCGGGCCCA 
ATGGGACCAC CAGGGCTGCC AGGATCAGAT GGTCCAAAAG GAGACCCTGG CCCTCCAGGT 
CTAGACATCC CAGGCCCACC AGGAGACCCA GGAGCTCCAG GTTATCAAGG TCTTCCAGGT 
ACTATTGGAA CCCCAGGACC ACCTGGAGGA CCTGGTCGCG ATGGTTTGCC TGGCTTGCCA 
GGTCAGAAAG GAGACATGGG TCCCAATGGA CCTCCAGGAC CTTCTGGAGG ACCGGGAGCC 
CCAGGAGGAC CTGGTGCTCC TGGATTTAAA GGTGAACCAG GATTTCCAGG TAGAGACGGT 
GCACCAGGTG GTCCTGGTGC TAAAGGAGAG AGGGGAGACA TGGGCCTCCA GGGACCGCCA 
GGACCCAGTG TTCCATCGCA GCTCTTAAAG GGACAAAAAG GAGATCCTGG ACCTTCAGGT 
GGAACAGGTT TTCCGGGTCC AAAAGGTGCT ACTGGTCTCC CTGGTGACCC TGGGTTTCCA 
GGAACAGATG GCAGTCCTGG CCGCCCTGGA CCTCCAGGTT CTAAGGGAGA TTCTGGTCGC 
CCAGGAGGTC CAGGTATTCC TGGGTCTCCA GGACCTAAAG GCCAAATGGG AGACATGGGA 
ATCCCAGGAC CACCAGGGCT GAAAGGTGTT TCAGGAGTGT CGGGTATACC AGGATCCCCA 
GGACAGCCAG GACCACCTGG TAACCAAGGA CCCAAAGGTG AGCCAGGTTC TGCTGGCGTC 
GGACCACCTG GACTAACCGG ACAAAAGGGT GATCCAGGCC TGCCAGGGTA CCCAGGAAAT 
CCAGGCCTAA AAGGAAACCC GGGATTACCA GGTCCCACAG GTGTGACGGG AAGACCAGGC 
GATAAAGGTG ACCCCGGACT CCCAGGATTC CAAGGTCCTC CTGGTATCCC CGGTCCTAAG 
GGTCTTGACG GTGTGCCCGG TACCCCAGGT AGCTCAGGAG CTCCAGGTAG ACCAGGAGAA 
CCTGGTCCAC AAGGACAGTC TGGTCCACCA GGAGAGAAAG GTCAGGCAGG TCGCGATGGG 
ATTCCAGGAC CAGCTGGAGT CAAAGGAGAA GCAGGAATTC CAGGTTACGG TGGTCCAGGT 
CCTGCTGGAA GTCCAGGACT GCCAGGTATC AAGGGAGACA CAGGTCCTCC TGGTGTACCC 
GGCAACCCTG GTTTTCCCGG CCCTAAAGGA GACCCAGGCT CACCTGGTGG CCCTGGTGAA 
CAAGGTGCCC CTGGCTTTCC TGGGCCTGCT GGGCTGTCTC TGCCAGGCCC TAAAGGTGCC 
ACAGGACCCC CTGGATTACC TGGAAGAGCA GGCCCTGCAG GTCCTCCAGG TTCACCTGGC 
CTACAAGGTC CTCGTGGACT CCCAGGAACT GGTGGCGTTA AGGGAGAAAA GGGTACTCTT 
GGCATGCCTG GAATTCCTGG CATCCCCGGA CAGAAGGGAG ATCCTGGTTT TCCAGGAAAT 
CCGGGCATAC CTGGTCCTAC TGGCCCCGCT GGAATAAAGG GAGATTCAGG ACAGCCTGGT 
GTTCCTGGAT TCCCTGGATT GAAGGGTGAT CTTGGATATC CTGGCCCCAG TGGCCCTCCT 
GGAGATCGTG GATTACCTGG AGAACGGGGC ACTCCGGGTG ATCCCGGTGA AGGTTCGCCT 
CCCATTGTGC TAAAGGGAGA ACGGGGACCC CCAGGTCTTC AGGGCCTACC TGGTTCTCCA 
GGACCACCTG GTTTGCCTGG CCAACCTGGA GGCTCAGGCA TCAAAGGTGA CCCTGGTGGA 
CCTGGGAGCG ATGGTCCTCC TGGCTTCAAC GGACCTGCCG GCAGGAAGGG AGACATTGGT 
CCGGCAGGTC AGCCTGGTCA GCCTGGCAAC CCTGGTCCCC GTGGTTCGGA TGGCCCACAA 
GGTCCTCCCG GTGTCCCAGG ATCAGTTTCT GCTGCTCACG GTTTCCTCAT CACCCGCCAC 
AGCCAGACCA CCGATGACCC AGAGTGCCCC ACTGGAACTA AGTTTATCTA TAACGGATAC 
TCTCTGTTGT ACGTGCAGGG CAACGAGCGG GCACACGGAC AGGACCTTGG CACAGCCGGT 
AGCTGCCTGC GCAGATTTAG CACCATGCCC TTCATGTTCT GCAATATCAA CAACGTCTGC 
AACTTCGCAT CCCGTAACGA CTACTCCTAC TGGCTGTCAA CGGACAGACC CATGCCCGAA 
AACATGGCCC CCTTCGTGGG AGAGAATATC AGGCCTCATG TCAGCAGATG TAGAGTCTGT 
GAAGCTCCCG CCATGGTGAT TGCAATTCAC AGTCAGACTA TCCAGATTCC TTCTTGCCCT 
GAAAACTGGG AAGTACTGTG GATTGGATAC TCCTTTATGA TGCACACCAG TGCGGGTGCA 
GAGGGCTCCG GTCAGGCGCT CGCCTCCCCC GGCTCCTGCC TGGAGGAGTT CCGCAGCTCC 
CCTTTCATCG AGTGCCACGG CAGAGGGACA TGTAACTTCT ATGCCAACTC CTACAGCTTC 
TGGCTGGCCA CCGTGGAAAC TTCTGAGATG TTCAGAAAGC CCCAGTCTGA GACCTACAAG 
GCAGGTAGCC TACGGACACG TGTGAGCCGC TGTGCAGTCT GCATGAAGCG CACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014268794.1
CDS	194..5230
Translation	MSKDSKCLRQLGATLLLVLFAGVQRSDSAACHGCAGSKCDCSGVKGEKGERGFPGLTGQPGVPGFPGPEGPIGPRGEKGSDGPQGPGGPKGIRGPPGTPGFPGTPGLPGLPGQDGPPGPRGVPGCNGTKGDRGFPGSPGIPGQPGLQGPPGLPGQKGDPGDVISANRFGEKGAVGLPGLPGGPGRPGPPGIQGPVGPPGPRGLEGRPGAPGPPGPKGNMGLNFQGPKGEKGAPGLQGPPGPPGQVGEQTKPAETEIQRGDKGDQGPPGPKGDPGLPGPPGPYGGQKGEKGEPGELGKRGKPGKDGDPGSPGYPGPPGETGQPGLPGRDGETGQKGDRGFPGPPGQVIGGPIGNQVGPKGEPGLPGTPGLKGDRGVQGLTGPPGEPGFTGTGSGGFPGPQGFPGERGQKGEIGPPGNFLPGPPGRAGSPGSPGPPGPRGLPGTSSGQDCIPGLPGVPGAQGGRGFPGEIGQKGEKGDTCVNCLGSVNPIPGPQGPPGPPGFPGPPGVQGFKGDRGFPGTPGSVGPSGPPGAQGPPGLNGEKGDPGDPIRVNGLSGEKGDTGFPGPPGLPGLDGAPGPPGPKGAPVLLVRGERGLPGEPGFPGNPGDRGPPGLPGFGNPGPREKGVQGVSGRPGPVGAPGTKGEPGQTMTEKGAKGERGRDGEPGQPGSPGIPGQPGQPGFPGLSGAKGEPGIPGIGLPGPPGPKGFPGVGGQPGAPGGPGRPGIDGRPGELGFPGPKGEPGLGLPGPPGLTGAPGPKGSIGPKGDPGFPGNPGLSGRLGADGAPGFKGEPGTPGTPGSRGPPGPPSFGGTGPPGLPGPPGPMGPPGLPGSDGPKGDPGPPGLDIPGPPGDPGAPGYQGLPGTIGTPGPPGGPGRDGLPGLPGQKGDMGPNGPPGPSGGPGAPGGPGAPGFKGEPGFPGRDGAPGGPGAKGERGDMGLQGPPGPSVPSQLLKGQKGDPGPSGGTGFPGPKGATGLPGDPGFPGTDGSPGRPGPPGSKGDSGRPGGPGIPGSPGPKGQMGDMGIPGPPGLKGVSGVSGIPGSPGQPGPPGNQGPKGEPGSAGVGPPGLTGQKGDPGLPGYPGNPGLKGNPGLPGPTGVTGRPGDKGDPGLPGFQGPPGIPGPKGLDGVPGTPGSSGAPGRPGEPGPQGQSGPPGEKGQAGRDGIPGPAGVKGEAGIPGYGGPGPAGSPGLPGIKGDTGPPGVPGNPGFPGPKGDPGSPGGPGEQGAPGFPGPAGLSLPGPKGATGPPGLPGRAGPAGPPGSPGLQGPRGLPGTGGVKGEKGTLGMPGIPGIPGQKGDPGFPGNPGIPGPTGPAGIKGDSGQPGVPGFPGLKGDLGYPGPSGPPGDRGLPGERGTPGDPGEGSPPIVLKGERGPPGLQGLPGSPGPPGLPGQPGGSGIKGDPGGPGSDGPPGFNGPAGRKGDIGPAGQPGQPGNPGPRGSDGPQGPPGVPGSVSAAHGFLITRHSQTTDDPECPTGTKFIYNGYSLLYVQGNERAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTDRPMPENMAPFVGENIRPHVSRCRVCEAPAMVIAIHSQTIQIPSCPENWEVLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSSPFIECHGRGTCNFYANSYSFWLATVETSEMFRKPQSETYKAGSLRTRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_014413308.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra collagen type IV alpha 5 chain (col4a5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014413308.1

ORF Insert Sequence:

ATGAGTAAAG ACTCAAAGTG CCTGCGACAG CTCGGTGCGA CTCTGCTACT AGTCCTGTTC 
GCCGGGGTGC AGCGATCCGA CTCTGCGGCT TGCCATGGAT GTGCAGGATC CAAATGTGAC 
TGCAGTGGAG TGAAAGGAGA AAAGGGTGAA AGGGGTTTCC CAGGTTTGAC AGGACAGCCA 
GGCGTCCCAG GATTCCCTGG ACCAGAAGGA CCAATTGGGC CCAGAGGAGA GAAGGGAAGT 
GATGGACCTC AAGGACCAGG TGGTCCAAAA GGAATTAGAG GACCTCCAGG AACACCAGGA 
TTTCCAGGAA CACCCGGACT CCCAGGTCTA CCTGGGCAGG ATGGTCCTCC AGGTCCAAGA 
GGAGTGCCGG GTTGCAATGG GACAAAGGGT GACAGGGGTT TCCCAGGAAG TCCAGGAATC 
CCTGGGCAGC CAGGACTTCA AGGTCCACCT GGTCTGCCAG GACAAAAGGG AGACCCAGGT 
GATGTTATCT CCGCCAATCG TTTCGGAGAG AAAGGAGCAG TGGGTTTACC CGGTTTGCCA 
GGAGGTCCTG GCCGACCTGG ACCTCCAGGT ATACAAGGTC CGGTAGGTCC CCCAGGACCC 
AGAGGCCTTG AGGGTCGTCC AGGTGCTCCT GGTCCACCCG GGCCTAAGGG GAATATGGGT 
TTGAATTTCC AAGGACCCAA AGGAGAAAAG GGTGCACCAG GTTTGCAAGG ACCTCCTGGT 
CCTCCAGGAC AGGTTGGGGA GCAGACAAAG CCGGCTGAGA CTGAAATTCA GAGAGGAGAC 
AAGGGTGACC AAGGACCTCC TGGCCCTAAA GGTGATCCGG GTTTACCGGG ACCTCCTGGT 
CCCTATGGTG GGCAGAAAGG AGAAAAGGGA GAACCAGGAG AGCTGGGAAA ACGAGGAAAA 
CCAGGCAAAG ATGGTGATCC TGGTTCTCCA GGATACCCAG GGCCCCCTGG AGAGACAGGC 
CAGCCTGGTC TTCCAGGGAG GGATGGTGAG ACAGGACAAA AAGGTGATCG TGGATTCCCG 
GGTCCACCAG GGCAGGTGAT TGGCGGGCCA ATAGGGAATC AGGTGGGGCC TAAAGGTGAA 
CCTGGCTTAC CTGGAACACC TGGACTCAAA GGAGACAGAG GAGTACAAGG TTTGACAGGC 
CCTCCAGGAG AACCGGGATT CACAGGAACC GGTAGTGGGG GTTTTCCTGG ACCTCAAGGT 
TTCCCAGGAG AGCGAGGTCA GAAGGGAGAG ATTGGCCCTC CAGGAAACTT TCTGCCAGGC 
CCCCCAGGTC GTGCAGGGTC TCCTGGATCC CCTGGTCCGC CAGGGCCCCG TGGACTTCCA 
GGCACTTCCT CAGGTCAAGA CTGCATACCA GGACTTCCTG GGGTTCCTGG TGCACAAGGA 
GGAAGAGGTT TCCCTGGAGA GATAGGACAG AAAGGTGAGA AAGGTGACAC CTGTGTGAAC 
TGCCTAGGAA GCGTCAATCC CATCCCTGGA CCTCAAGGAC CTCCAGGACC TCCTGGATTT 
CCTGGACCTC CTGGCGTCCA AGGTTTCAAG GGAGACAGAG GTTTTCCAGG AACACCTGGC 
TCTGTCGGCC CCAGCGGTCC TCCTGGTGCT CAAGGTCCTC CCGGCCTAAA TGGAGAGAAG 
GGAGATCCAG GTGATCCCAT CAGAGTTAAT GGTTTGAGTG GAGAGAAGGG TGACACTGGT 
TTCCCTGGAC CACCTGGTCT GCCCGGTCTG GACGGGGCGC CTGGGCCTCC AGGGCCTAAA 
GGAGCTCCTG TTTTGTTGGT GAGGGGAGAA AGAGGCCTCC CCGGTGAACC AGGTTTCCCT 
GGTAATCCAG GAGACAGGGG TCCCCCAGGT CTTCCTGGCT TTGGAAATCC AGGACCTCGT 
GAGAAAGGTG TCCAGGGTGT CTCAGGAAGA CCGGGACCTG TTGGTGCACC AGGAACTAAG 
GGTGAACCTG GTCAGACAAT GACAGAGAAA GGGGCCAAAG GAGAAAGAGG TCGGGATGGC 
GAGCCTGGGC AGCCTGGTTC ACCAGGTATA CCAGGTCAGC CTGGACAGCC TGGTTTTCCC 
GGTTTATCGG GAGCGAAGGG TGAACCCGGA ATCCCAGGCA TTGGACTGCC AGGACCTCCA 
GGACCAAAAG GATTCCCGGG TGTTGGCGGG CAACCAGGAG CCCCTGGAGG ACCAGGAAGA 
CCAGGGATAG ATGGACGTCC CGGTGAGCTG GGATTTCCTG GACCAAAGGG TGAACCTGGT 
CTTGGACTTC CTGGTCCTCC TGGTTTAACG GGTGCACCGG GCCCTAAAGG TTCCATTGGG 
CCTAAGGGAG ATCCTGGTTT CCCTGGTAAC CCCGGTTTGT CAGGACGGCT TGGAGCTGAT 
GGAGCTCCAG GATTCAAAGG TGAGCCTGGT ACACCTGGTA CACCTGGATC TCGTGGCCCC 
CCTGGACCCC CCTCTTTTGG TGGAACGGGC CCACCAGGTC TCCCAGGACC TCCGGGCCCA 
ATGGGACCAC CAGGGCTGCC AGGATCAGAT GGTCCAAAAG GAGACCCTGG CCCTCCAGGT 
CTAGACATCC CAGGCCCACC AGGAGACCCA GGAGCTCCAG GTTATCAAGG TCTTCCAGGT 
ACTATTGGAA CCCCAGGACC ACCTGGAGGA CCTGGTCGCG ATGGTTTGCC TGGCTTGCCA 
GGTCAGAAAG GAGACATGGG TCCCAATGGA CCTCCAGGAC CTTCTGGAGG ACCGGGAGCC 
CCAGGAGGAC CTGGTGCTCC TGGATTTAAA GGTGAACCAG GATTTCCAGG TAGAGACGGT 
GCACCAGGTG GTCCTGGTGC TAAAGGAGAG AGGGGAGACA TGGGCCTCCA GGGACCGCCA 
GGACCCAGTG TTCCATCGCA GCTCTTAAAG GGACAAAAAG GAGATCCTGG ACCTTCAGGT 
GGAACAGGTT TTCCGGGTCC AAAAGGTGCT ACTGGTCTCC CTGGTGACCC TGGGTTTCCA 
GGAACAGATG GCAGTCCTGG CCGCCCTGGA CCTCCAGGTT CTAAGGGAGA TTCTGGTCGC 
CCAGGAGGTC CAGGTATTCC TGGGTCTCCA GGACCTAAAG GCCAAATGGG AGACATGGGA 
ATCCCAGGAC CACCAGGGCT GAAAGGTGTT TCAGGAGTGT CGGGTATACC AGGATCCCCA 
GGACAGCCAG GACCACCTGG TAACCAAGGA CCCAAAGGTG AGCCAGGTTC TGCTGGCGTC 
GGACCACCTG GACTAACCGG ACAAAAGGGT GATCCAGGCC TGCCAGGGTA CCCAGGAAAT 
CCAGGCCTAA AAGGAAACCC GGGATTACCA GGTCCCACAG GTGTGACGGG AAGACCAGGC 
GATAAAGGTG ACCCCGGACT CCCAGGATTC CAAGGTCCTC CTGGTATCCC CGGTCCTAAG 
GGTCTTGACG GTGTGCCCGG TACCCCAGGT AGCTCAGGAG CTCCAGGTAG ACCAGGAGAA 
CCTGGTCCAC AAGGACAGTC TGGTCCACCA GGAGAGAAAG GTCAGGCAGG TCGCGATGGG 
ATTCCAGGAC CAGCTGGAGT CAAAGGAGAA GCAGGAATTC CAGGTTACGG TGGTCCAGGT 
CCTGCTGGAA GTCCAGGACT GCCAGGTATC AAGGGAGACA CAGGTCCTCC TGGTGTACCC 
GGCAACCCTG GTTTTCCCGG CCCTAAAGGA GACCCAGGCT CACCTGGTGG CCCTGGTGAA 
CAAGGTGCCC CTGGCTTTCC TGGGCCTGCT GGGCTGTCTC TGCCAGGCCC TAAAGGTGCC 
ACAGGACCCC CTGGATTACC TGGAAGAGCA GGCCCTGCAG GTCCTCCAGG TTCACCTGGC 
CTACAAGGTC CTCGTGGACT CCCAGGAACT GGTGGCGTTA AGGGAGAAAA GGGTACTCTT 
GGCATGCCTG GAATTCCTGG CATCCCCGGA CAGAAGGGAG ATCCTGGTTT TCCAGGAAAT 
CCGGGCATAC CTGGTCCTAC TGGCCCCGCT GGAATAAAGG GAGATTCAGG ACAGCCTGGT 
GTTCCTGGAT TCCCTGGATT GAAGGGTGAT CTTGGATATC CTGGCCCCAG TGGCCCTCCT 
GGAGATCGTG GATTACCTGG AGAACGGGGC ACTCCGGGTG ATCCCGGTGA AGGTTCGCCT 
CCCATTGTGC TAAAGGGAGA ACGGGGACCC CCAGGTCTTC AGGGCCTACC TGGTTCTCCA 
GGACCACCTG GTTTGCCTGG CCAACCTGGA GGCTCAGGCA TCAAAGGTGA CCCTGGTGGA 
CCTGGGAGCG ATGGTCCTCC TGGCTTCAAC GGACCTGCCG GCAGGAAGGG AGACATTGGT 
CCGGCAGGTC AGCCTGGTCA GCCTGGCAAC CCTGGTCCCC GTGGTTCGGA TGGCCCACAA 
GGTCCTCCCG GTGTCCCAGG ATCAGTTTCT GCTGCTCACG GTTTCCTCAT CACCCGCCAC 
AGCCAGACCA CCGATGACCC AGAGTGCCCC ACTGGAACTA AGTTTATCTA TAACGGATAC 
TCTCTGTTGT ACGTGCAGGG CAACGAGCGG GCACACGGAC AGGACCTTGG CACAGCCGGT 
AGCTGCCTGC GCAGATTTAG CACCATGCCC TTCATGTTCT GCAATATCAA CAACGTCTGC 
AACTTCGCAT CCCGTAACGA CTACTCCTAC TGGCTGTCAA CGGACAGACC CATGCCCGAA 
AACATGGCCC CCTTCGTGGG AGAGAATATC AGGCCTCATG TCAGCAGATG TAGAGTCTGT 
GAAGCTCCCG CCATGGTGAT TGCAATTCAC AGTCAGACTA TCCAGATTCC TTCTTGCCCT 
GAAAACTGGG AAGTACTGTG GATTGGATAC TCCTTTATGA TGCACACCAG TGCGGGTGCA 
GAGGGCTCCG GTCAGGCGCT CGCCTCCCCC GGCTCCTGCC TGGAGGAGTT CCGCAGCTCC 
CCTTTCATCG AGTGCCACGG CAGAGGGACA TGTAACTTCT ATGCCAACTC CTACAGCTTC 
TGGCTGGCCA CCGTGGAAAC TTCTGAGATG TTCAGAAAGC CCCAGTCTGA GACCTACAAG 
GCAGGTAGCC TACGGACACG TGTGAGCCGC TGTGCAGTCT GCATGAAGCG CACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

col4a5 ( XM_014413309.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_014268795.1 Maylandia zebra collagen type IV alpha 5 chain (col4a5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMa24138
Clone ID Related Accession (Same CDS sequence)	XM_014413309.1
Accession Version	XM_014413309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1511884800000
Organism	Maylandia zebra(zebra mbuna)
Product	collagen alpha-5(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447357.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAAAG ACTCAAAGTG CCTGCGACAG CTCGGTGCGA CTCTGCTACT AGTCCTGTTC 
GCCGGGGTGC AGCGATCCGA CTCTGCGGCT TGCCATGGAT GTGCAGGATC CAAATGTGAC 
TGCAGTGGAG TGAAAGGAGA AAAGGGTGAA AGGGGTTTCC CAGGTTTGAC AGGACAGCCA 
GGCGTCCCAG GATTCCCTGG ACCAGAAGGA CCAATTGGGC CCAGAGGAGA GAAGGGAAGT 
GATGGACCTC AAGGACCAGG TGGTCCAAAA GGAATTAGAG GACCTCCAGG AACACCAGGA 
TTTCCAGGAA CACCCGGACT CCCAGGTCTA CCTGGGCAGG ATGGTCCTCC AGGTCCAAGA 
GGAGTGCCGG GTTGCAATGG GACAAAGGGT GACAGGGGTT TCCCAGGAAG TCCAGGAATC 
CCTGGGCAGC CAGGACTTCA AGGTCCACCT GGTCTGCCAG GACAAAAGGG AGACCCAGGT 
GATGTTATCT CCGCCAATCG TTTCGGAGAG AAAGGAGCAG TGGGTTTACC CGGTTTGCCA 
GGAGGTCCTG GCCGACCTGG ACCTCCAGGT ATACAAGGTC CGGTAGGTCC CCCAGGACCC 
AGAGGCCTTG AGGGTCGTCC AGGTGCTCCT GGTCCACCCG GGCCTAAGGG GAATATGGGT 
TTGAATTTCC AAGGACCCAA AGGAGAAAAG GGTGCACCAG GTTTGCAAGG ACCTCCTGGT 
CCTCCAGGAC AGGTTGGGGA GCAGACAAAG CCGGCTGAGA CTGAAATTCA GAGAGGAGAC 
AAGGGTGACC AAGGACCTCC TGGCCCTAAA GGTGATCCGG GTTTACCGGG ACCTCCTGGT 
CCCTATGGTG GGCAGAAAGG AGAAAAGGGA GAACCAGGAG AGCTGGGAAA ACGAGGAAAA 
CCAGGCAAAG ATGGTGATCC TGGTTCTCCA GGATACCCAG GGCCCCCTGG AGAGACAGGC 
CAGCCTGGTC TTCCAGGGAG GGATGGTGAG ACAGGACAAA AAGGTGATCG TGGATTCCCG 
GGTCCACCAG GGCAGGTGAT TGGCGGGCCA ATAGGGAATC AGGTGGGGCC TAAAGGTGAA 
CCTGGCTTAC CTGGAACACC TGGACTCAAA GGAGACAGAG GAGTACAAGG TTTGACAGGC 
CCTCCAGGAG AACCGGGATT CACAGGAACC GGTAGTGGGG GTTTTCCTGG ACCTCAAGGT 
TTCCCAGGAG AGCGAGGTCA GAAGGGAGAG ATTGGCCCTC CAGGAAACTT TCTGCCAGGC 
CCCCCAGGTC GTGCAGGGTC TCCTGGATCC CCTGGTCCGC CAGGGCCCCG TGGACTTCCA 
GGCACTTCCT CAGGTCAAGA CTGCATACCA GGACTTCCTG GGGTTCCTGG TGCACAAGGA 
GGAAGAGGTT TCCCTGGAGA GATAGGACAG AAAGGTGAGA AAGGTGACAC CTGTGTGAAC 
TGCCTAGGAA GCGTCAATCC CATCCCTGGA CCTCAAGGAC CTCCAGGACC TCCTGGATTT 
CCTGGACCTC CTGGCGTCCA AGGTTTCAAG GGAGACAGAG GTTTTCCAGG AACACCTGGC 
TCTGTCGGCC CCAGCGGTCC TCCTGGTGCT CAAGGTCCTC CCGGCCTAAA TGGAGAGAAG 
GGAGATCCAG GTGATCCCAT CAGAGTTAAT GGTTTGAGTG GAGAGAAGGG TGACACTGGT 
TTCCCTGGAC CACCTGGTCT GCCCGGTCTG GACGGGGCGC CTGGGCCTCC AGGGCCTAAA 
GGAGCTCCTG TTTTGTTGGT GAGGGGAGAA AGAGGCCTCC CCGGTGAACC AGGTTTCCCT 
GGTAATCCAG GAGACAGGGG TCCCCCAGGT CTTCCTGGCT TTGGAAATCC AGGACCTCGT 
GAGAAAGGTG TCCAGGGTGT CTCAGGAAGA CCGGGACCTG TTGGTGCACC AGGAACTAAG 
GGTGAACCTG GTCAGACAAT GACAGAGAAA GGGGCCAAAG GAGAAAGAGG TCGGGATGGC 
GAGCCTGGGC AGCCTGGTTC ACCAGGTATA CCAGGTCAGC CTGGACAGCC TGGTTTTCCC 
GGTTTATCGG GAGCGAAGGG TGAACCCGGA ATCCCAGGCA TTGGACTGCC AGGACCTCCA 
GGACCAAAAG GATTCCCGGG TGTTGGCGGG CAACCAGGAG CCCCTGGAGG ACCAGGAAGA 
CCAGGGATAG ATGGACGTCC CGGTGAGCTG GGATTTCCTG GACCAAAGGG TGAACCTGGT 
CTTGGACTTC CTGGTCCTCC TGGTTTAACG GGTGCACCGG GCCCTAAAGG TTCCATTGGG 
CCTAAGGGAG ATCCTGGTTT CCCTGGTAAC CCCGGTTTGT CAGGACGGCT TGGAGCTGAT 
GGAGCTCCAG GATTCAAAGG TGAGCCTGGT ACACCTGGTA CACCTGGATC TCGTGGCCCC 
CCTGGACCCC CCTCTTTTGG TGGAACGGGC CCACCAGGTC TCCCAGGACC TCCGGGCCCA 
ATGGGACCAC CAGGGCTGCC AGGATCAGAT GGTCCAAAAG GAGACCCTGG CCCTCCAGGT 
CTAGACATCC CAGGCCCACC AGGAGACCCA GGAGCTCCAG GTTATCAAGG TCTTCCAGGT 
ACTATTGGAA CCCCAGGACC ACCTGGAGGA CCTGGTCGCG ATGGTTTGCC TGGCTTGCCA 
GGTCAGAAAG GAGACATGGG TCCCAATGGA CCTCCAGGAC CTTCTGGAGG ACCGGGAGCC 
CCAGGAGGAC CTGGTGCTCC TGGATTTAAA GGTGAACCAG GATTTCCAGG TAGAGACGGT 
GCACCAGGTG GTCCTGGTGC TAAAGGAGAG AGGGGAGACA TGGGCCTCCA GGGACCGCCA 
GGACCCAGTG TTCCATCGCA GCTCTTAAAG GGACAAAAAG GAGATCCTGG ACCTTCAGGT 
GGAACAGGTT TTCCGGGTCC AAAAGGTGCT ACTGGTCTCC CTGGTGACCC TGGGTTTCCA 
GGAACAGATG GCAGTCCTGG CCGCCCTGGA CCTCCAGGTT CTAAGGGAGA TTCTGGTCGC 
CCAGGAGGTC CAGGTATTCC TGGGTCTCCA GGACCTAAAG GCCAAATGGG AGACATGGGA 
ATCCCAGGAC CACCAGGGCT GAAAGGTGTT TCAGGAGTGT CGGGTATACC AGGATCCCCA 
GGACAGCCAG GACCACCTGG TAACCAAGGA CCCAAAGGTG AGCCAGGTTC TGCTGGCGTC 
GGACCACCTG GACTAACCGG ACAAAAGGGT GATCCAGGCC TGCCAGGGTA CCCAGGAAAT 
CCAGGCCTAA AAGGAAACCC GGGATTACCA GGTCCCACAG GTGTGACGGG AAGACCAGGC 
GATAAAGGTG ACCCCGGACT CCCAGGATTC CAAGGTCCTC CTGGTATCCC CGGTCCTAAG 
GGTCTTGACG GTGTGCCCGG TACCCCAGGT AGCTCAGGAG CTCCAGGTAG ACCAGGAGAA 
CCTGGTCCAC AAGGACAGTC TGGTCCACCA GGAGAGAAAG GTCAGGCAGG TCGCGATGGG 
ATTCCAGGAC CAGCTGGAGT CAAAGGAGAA GCAGGAATTC CAGGTTACGG TGGTCCAGGT 
CCTGCTGGAA GTCCAGGACT GCCAGGTATC AAGGGAGACA CAGGTCCTCC TGGTGTACCC 
GGCAACCCTG GTTTTCCCGG CCCTAAAGGA GACCCAGGCT CACCTGGTGG CCCTGGTGAA 
CAAGGTGCCC CTGGCTTTCC TGGGCCTGCT GGGCTGTCTC TGCCAGGCCC TAAAGGTGCC 
ACAGGACCCC CTGGATTACC TGGAAGAGCA GGTTCACCTG GCCTACAAGG TCCTCGTGGA 
CTCCCAGGAA CTGGTGGCGT TAAGGGAGAA AAGGGTACTC TTGGCATGCC TGGAATTCCT 
GGCATCCCCG GACAGAAGGG AGATCCTGGT TTTCCAGGAA ATCCGGGCAT ACCTGGTCCT 
ACTGGCCCCG CTGGAATAAA GGGAGATTCA GGACAGCCTG GTGTTCCTGG ATTCCCTGGA 
TTGAAGGGTG ATCTTGGATA TCCTGGCCCC AGTGGCCCTC CTGGAGATCG TGGATTACCT 
GGAGAACGGG GCACTCCGGG TGATCCCGGT GAAGGTTCGC CTCCCATTGT GCTAAAGGGA 
GAACGGGGAC CCCCAGGTCT TCAGGGCCTA CCTGGTTCTC CAGGACCACC TGGTTTGCCT 
GGCCAACCTG GAGGCTCAGG CATCAAAGGT GACCCTGGTG GACCTGGGAG CGATGGTCCT 
CCTGGCTTCA ACGGACCTGC CGGCAGGAAG GGAGACATTG GTCCGGCAGG TCAGCCTGGT 
CAGCCTGGCA ACCCTGGTCC CCGTGGTTCG GATGGCCCAC AAGGTCCTCC CGGTGTCCCA 
GGATCAGTTT CTGCTGCTCA CGGTTTCCTC ATCACCCGCC ACAGCCAGAC CACCGATGAC 
CCAGAGTGCC CCACTGGAAC TAAGTTTATC TATAACGGAT ACTCTCTGTT GTACGTGCAG 
GGCAACGAGC GGGCACACGG ACAGGACCTT GGCACAGCCG GTAGCTGCCT GCGCAGATTT 
AGCACCATGC CCTTCATGTT CTGCAATATC AACAACGTCT GCAACTTCGC ATCCCGTAAC 
GACTACTCCT ACTGGCTGTC AACGGACAGA CCCATGCCCG AAAACATGGC CCCCTTCGTG 
GGAGAGAATA TCAGGCCTCA TGTCAGCAGA TGTAGAGTCT GTGAAGCTCC CGCCATGGTG 
ATTGCAATTC ACAGTCAGAC TATCCAGATT CCTTCTTGCC CTGAAAACTG GGAAGTACTG 
TGGATTGGAT ACTCCTTTAT GATGCACACC AGTGCGGGTG CAGAGGGCTC CGGTCAGGCG 
CTCGCCTCCC CCGGCTCCTG CCTGGAGGAG TTCCGCAGCT CCCCTTTCAT CGAGTGCCAC 
GGCAGAGGGA CATGTAACTT CTATGCCAAC TCCTACAGCT TCTGGCTGGC CACCGTGGAA 
ACTTCTGAGA TGTTCAGAAA GCCCCAGTCT GAGACCTACA AGGCAGGTAG CCTACGGACA 
CGTGTGAGCC GCTGTGCAGT CTGCATGAAG CGCACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014268795.1
CDS	194..5212
Translation	MSKDSKCLRQLGATLLLVLFAGVQRSDSAACHGCAGSKCDCSGVKGEKGERGFPGLTGQPGVPGFPGPEGPIGPRGEKGSDGPQGPGGPKGIRGPPGTPGFPGTPGLPGLPGQDGPPGPRGVPGCNGTKGDRGFPGSPGIPGQPGLQGPPGLPGQKGDPGDVISANRFGEKGAVGLPGLPGGPGRPGPPGIQGPVGPPGPRGLEGRPGAPGPPGPKGNMGLNFQGPKGEKGAPGLQGPPGPPGQVGEQTKPAETEIQRGDKGDQGPPGPKGDPGLPGPPGPYGGQKGEKGEPGELGKRGKPGKDGDPGSPGYPGPPGETGQPGLPGRDGETGQKGDRGFPGPPGQVIGGPIGNQVGPKGEPGLPGTPGLKGDRGVQGLTGPPGEPGFTGTGSGGFPGPQGFPGERGQKGEIGPPGNFLPGPPGRAGSPGSPGPPGPRGLPGTSSGQDCIPGLPGVPGAQGGRGFPGEIGQKGEKGDTCVNCLGSVNPIPGPQGPPGPPGFPGPPGVQGFKGDRGFPGTPGSVGPSGPPGAQGPPGLNGEKGDPGDPIRVNGLSGEKGDTGFPGPPGLPGLDGAPGPPGPKGAPVLLVRGERGLPGEPGFPGNPGDRGPPGLPGFGNPGPREKGVQGVSGRPGPVGAPGTKGEPGQTMTEKGAKGERGRDGEPGQPGSPGIPGQPGQPGFPGLSGAKGEPGIPGIGLPGPPGPKGFPGVGGQPGAPGGPGRPGIDGRPGELGFPGPKGEPGLGLPGPPGLTGAPGPKGSIGPKGDPGFPGNPGLSGRLGADGAPGFKGEPGTPGTPGSRGPPGPPSFGGTGPPGLPGPPGPMGPPGLPGSDGPKGDPGPPGLDIPGPPGDPGAPGYQGLPGTIGTPGPPGGPGRDGLPGLPGQKGDMGPNGPPGPSGGPGAPGGPGAPGFKGEPGFPGRDGAPGGPGAKGERGDMGLQGPPGPSVPSQLLKGQKGDPGPSGGTGFPGPKGATGLPGDPGFPGTDGSPGRPGPPGSKGDSGRPGGPGIPGSPGPKGQMGDMGIPGPPGLKGVSGVSGIPGSPGQPGPPGNQGPKGEPGSAGVGPPGLTGQKGDPGLPGYPGNPGLKGNPGLPGPTGVTGRPGDKGDPGLPGFQGPPGIPGPKGLDGVPGTPGSSGAPGRPGEPGPQGQSGPPGEKGQAGRDGIPGPAGVKGEAGIPGYGGPGPAGSPGLPGIKGDTGPPGVPGNPGFPGPKGDPGSPGGPGEQGAPGFPGPAGLSLPGPKGATGPPGLPGRAGSPGLQGPRGLPGTGGVKGEKGTLGMPGIPGIPGQKGDPGFPGNPGIPGPTGPAGIKGDSGQPGVPGFPGLKGDLGYPGPSGPPGDRGLPGERGTPGDPGEGSPPIVLKGERGPPGLQGLPGSPGPPGLPGQPGGSGIKGDPGGPGSDGPPGFNGPAGRKGDIGPAGQPGQPGNPGPRGSDGPQGPPGVPGSVSAAHGFLITRHSQTTDDPECPTGTKFIYNGYSLLYVQGNERAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTDRPMPENMAPFVGENIRPHVSRCRVCEAPAMVIAIHSQTIQIPSCPENWEVLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSSPFIECHGRGTCNFYANSYSFWLATVETSEMFRKPQSETYKAGSLRTRVSRCAVCMKRT

Target ORF information:

RefSeq Version	XM_014413309.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra collagen type IV alpha 5 chain (col4a5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014413309.1

ORF Insert Sequence:

ATGAGTAAAG ACTCAAAGTG CCTGCGACAG CTCGGTGCGA CTCTGCTACT AGTCCTGTTC 
GCCGGGGTGC AGCGATCCGA CTCTGCGGCT TGCCATGGAT GTGCAGGATC CAAATGTGAC 
TGCAGTGGAG TGAAAGGAGA AAAGGGTGAA AGGGGTTTCC CAGGTTTGAC AGGACAGCCA 
GGCGTCCCAG GATTCCCTGG ACCAGAAGGA CCAATTGGGC CCAGAGGAGA GAAGGGAAGT 
GATGGACCTC AAGGACCAGG TGGTCCAAAA GGAATTAGAG GACCTCCAGG AACACCAGGA 
TTTCCAGGAA CACCCGGACT CCCAGGTCTA CCTGGGCAGG ATGGTCCTCC AGGTCCAAGA 
GGAGTGCCGG GTTGCAATGG GACAAAGGGT GACAGGGGTT TCCCAGGAAG TCCAGGAATC 
CCTGGGCAGC CAGGACTTCA AGGTCCACCT GGTCTGCCAG GACAAAAGGG AGACCCAGGT 
GATGTTATCT CCGCCAATCG TTTCGGAGAG AAAGGAGCAG TGGGTTTACC CGGTTTGCCA 
GGAGGTCCTG GCCGACCTGG ACCTCCAGGT ATACAAGGTC CGGTAGGTCC CCCAGGACCC 
AGAGGCCTTG AGGGTCGTCC AGGTGCTCCT GGTCCACCCG GGCCTAAGGG GAATATGGGT 
TTGAATTTCC AAGGACCCAA AGGAGAAAAG GGTGCACCAG GTTTGCAAGG ACCTCCTGGT 
CCTCCAGGAC AGGTTGGGGA GCAGACAAAG CCGGCTGAGA CTGAAATTCA GAGAGGAGAC 
AAGGGTGACC AAGGACCTCC TGGCCCTAAA GGTGATCCGG GTTTACCGGG ACCTCCTGGT 
CCCTATGGTG GGCAGAAAGG AGAAAAGGGA GAACCAGGAG AGCTGGGAAA ACGAGGAAAA 
CCAGGCAAAG ATGGTGATCC TGGTTCTCCA GGATACCCAG GGCCCCCTGG AGAGACAGGC 
CAGCCTGGTC TTCCAGGGAG GGATGGTGAG ACAGGACAAA AAGGTGATCG TGGATTCCCG 
GGTCCACCAG GGCAGGTGAT TGGCGGGCCA ATAGGGAATC AGGTGGGGCC TAAAGGTGAA 
CCTGGCTTAC CTGGAACACC TGGACTCAAA GGAGACAGAG GAGTACAAGG TTTGACAGGC 
CCTCCAGGAG AACCGGGATT CACAGGAACC GGTAGTGGGG GTTTTCCTGG ACCTCAAGGT 
TTCCCAGGAG AGCGAGGTCA GAAGGGAGAG ATTGGCCCTC CAGGAAACTT TCTGCCAGGC 
CCCCCAGGTC GTGCAGGGTC TCCTGGATCC CCTGGTCCGC CAGGGCCCCG TGGACTTCCA 
GGCACTTCCT CAGGTCAAGA CTGCATACCA GGACTTCCTG GGGTTCCTGG TGCACAAGGA 
GGAAGAGGTT TCCCTGGAGA GATAGGACAG AAAGGTGAGA AAGGTGACAC CTGTGTGAAC 
TGCCTAGGAA GCGTCAATCC CATCCCTGGA CCTCAAGGAC CTCCAGGACC TCCTGGATTT 
CCTGGACCTC CTGGCGTCCA AGGTTTCAAG GGAGACAGAG GTTTTCCAGG AACACCTGGC 
TCTGTCGGCC CCAGCGGTCC TCCTGGTGCT CAAGGTCCTC CCGGCCTAAA TGGAGAGAAG 
GGAGATCCAG GTGATCCCAT CAGAGTTAAT GGTTTGAGTG GAGAGAAGGG TGACACTGGT 
TTCCCTGGAC CACCTGGTCT GCCCGGTCTG GACGGGGCGC CTGGGCCTCC AGGGCCTAAA 
GGAGCTCCTG TTTTGTTGGT GAGGGGAGAA AGAGGCCTCC CCGGTGAACC AGGTTTCCCT 
GGTAATCCAG GAGACAGGGG TCCCCCAGGT CTTCCTGGCT TTGGAAATCC AGGACCTCGT 
GAGAAAGGTG TCCAGGGTGT CTCAGGAAGA CCGGGACCTG TTGGTGCACC AGGAACTAAG 
GGTGAACCTG GTCAGACAAT GACAGAGAAA GGGGCCAAAG GAGAAAGAGG TCGGGATGGC 
GAGCCTGGGC AGCCTGGTTC ACCAGGTATA CCAGGTCAGC CTGGACAGCC TGGTTTTCCC 
GGTTTATCGG GAGCGAAGGG TGAACCCGGA ATCCCAGGCA TTGGACTGCC AGGACCTCCA 
GGACCAAAAG GATTCCCGGG TGTTGGCGGG CAACCAGGAG CCCCTGGAGG ACCAGGAAGA 
CCAGGGATAG ATGGACGTCC CGGTGAGCTG GGATTTCCTG GACCAAAGGG TGAACCTGGT 
CTTGGACTTC CTGGTCCTCC TGGTTTAACG GGTGCACCGG GCCCTAAAGG TTCCATTGGG 
CCTAAGGGAG ATCCTGGTTT CCCTGGTAAC CCCGGTTTGT CAGGACGGCT TGGAGCTGAT 
GGAGCTCCAG GATTCAAAGG TGAGCCTGGT ACACCTGGTA CACCTGGATC TCGTGGCCCC 
CCTGGACCCC CCTCTTTTGG TGGAACGGGC CCACCAGGTC TCCCAGGACC TCCGGGCCCA 
ATGGGACCAC CAGGGCTGCC AGGATCAGAT GGTCCAAAAG GAGACCCTGG CCCTCCAGGT 
CTAGACATCC CAGGCCCACC AGGAGACCCA GGAGCTCCAG GTTATCAAGG TCTTCCAGGT 
ACTATTGGAA CCCCAGGACC ACCTGGAGGA CCTGGTCGCG ATGGTTTGCC TGGCTTGCCA 
GGTCAGAAAG GAGACATGGG TCCCAATGGA CCTCCAGGAC CTTCTGGAGG ACCGGGAGCC 
CCAGGAGGAC CTGGTGCTCC TGGATTTAAA GGTGAACCAG GATTTCCAGG TAGAGACGGT 
GCACCAGGTG GTCCTGGTGC TAAAGGAGAG AGGGGAGACA TGGGCCTCCA GGGACCGCCA 
GGACCCAGTG TTCCATCGCA GCTCTTAAAG GGACAAAAAG GAGATCCTGG ACCTTCAGGT 
GGAACAGGTT TTCCGGGTCC AAAAGGTGCT ACTGGTCTCC CTGGTGACCC TGGGTTTCCA 
GGAACAGATG GCAGTCCTGG CCGCCCTGGA CCTCCAGGTT CTAAGGGAGA TTCTGGTCGC 
CCAGGAGGTC CAGGTATTCC TGGGTCTCCA GGACCTAAAG GCCAAATGGG AGACATGGGA 
ATCCCAGGAC CACCAGGGCT GAAAGGTGTT TCAGGAGTGT CGGGTATACC AGGATCCCCA 
GGACAGCCAG GACCACCTGG TAACCAAGGA CCCAAAGGTG AGCCAGGTTC TGCTGGCGTC 
GGACCACCTG GACTAACCGG ACAAAAGGGT GATCCAGGCC TGCCAGGGTA CCCAGGAAAT 
CCAGGCCTAA AAGGAAACCC GGGATTACCA GGTCCCACAG GTGTGACGGG AAGACCAGGC 
GATAAAGGTG ACCCCGGACT CCCAGGATTC CAAGGTCCTC CTGGTATCCC CGGTCCTAAG 
GGTCTTGACG GTGTGCCCGG TACCCCAGGT AGCTCAGGAG CTCCAGGTAG ACCAGGAGAA 
CCTGGTCCAC AAGGACAGTC TGGTCCACCA GGAGAGAAAG GTCAGGCAGG TCGCGATGGG 
ATTCCAGGAC CAGCTGGAGT CAAAGGAGAA GCAGGAATTC CAGGTTACGG TGGTCCAGGT 
CCTGCTGGAA GTCCAGGACT GCCAGGTATC AAGGGAGACA CAGGTCCTCC TGGTGTACCC 
GGCAACCCTG GTTTTCCCGG CCCTAAAGGA GACCCAGGCT CACCTGGTGG CCCTGGTGAA 
CAAGGTGCCC CTGGCTTTCC TGGGCCTGCT GGGCTGTCTC TGCCAGGCCC TAAAGGTGCC 
ACAGGACCCC CTGGATTACC TGGAAGAGCA GGTTCACCTG GCCTACAAGG TCCTCGTGGA 
CTCCCAGGAA CTGGTGGCGT TAAGGGAGAA AAGGGTACTC TTGGCATGCC TGGAATTCCT 
GGCATCCCCG GACAGAAGGG AGATCCTGGT TTTCCAGGAA ATCCGGGCAT ACCTGGTCCT 
ACTGGCCCCG CTGGAATAAA GGGAGATTCA GGACAGCCTG GTGTTCCTGG ATTCCCTGGA 
TTGAAGGGTG ATCTTGGATA TCCTGGCCCC AGTGGCCCTC CTGGAGATCG TGGATTACCT 
GGAGAACGGG GCACTCCGGG TGATCCCGGT GAAGGTTCGC CTCCCATTGT GCTAAAGGGA 
GAACGGGGAC CCCCAGGTCT TCAGGGCCTA CCTGGTTCTC CAGGACCACC TGGTTTGCCT 
GGCCAACCTG GAGGCTCAGG CATCAAAGGT GACCCTGGTG GACCTGGGAG CGATGGTCCT 
CCTGGCTTCA ACGGACCTGC CGGCAGGAAG GGAGACATTG GTCCGGCAGG TCAGCCTGGT 
CAGCCTGGCA ACCCTGGTCC CCGTGGTTCG GATGGCCCAC AAGGTCCTCC CGGTGTCCCA 
GGATCAGTTT CTGCTGCTCA CGGTTTCCTC ATCACCCGCC ACAGCCAGAC CACCGATGAC 
CCAGAGTGCC CCACTGGAAC TAAGTTTATC TATAACGGAT ACTCTCTGTT GTACGTGCAG 
GGCAACGAGC GGGCACACGG ACAGGACCTT GGCACAGCCG GTAGCTGCCT GCGCAGATTT 
AGCACCATGC CCTTCATGTT CTGCAATATC AACAACGTCT GCAACTTCGC ATCCCGTAAC 
GACTACTCCT ACTGGCTGTC AACGGACAGA CCCATGCCCG AAAACATGGC CCCCTTCGTG 
GGAGAGAATA TCAGGCCTCA TGTCAGCAGA TGTAGAGTCT GTGAAGCTCC CGCCATGGTG 
ATTGCAATTC ACAGTCAGAC TATCCAGATT CCTTCTTGCC CTGAAAACTG GGAAGTACTG 
TGGATTGGAT ACTCCTTTAT GATGCACACC AGTGCGGGTG CAGAGGGCTC CGGTCAGGCG 
CTCGCCTCCC CCGGCTCCTG CCTGGAGGAG TTCCGCAGCT CCCCTTTCAT CGAGTGCCAC 
GGCAGAGGGA CATGTAACTT CTATGCCAAC TCCTACAGCT TCTGGCTGGC CACCGTGGAA 
ACTTCTGAGA TGTTCAGAAA GCCCCAGTCT GAGACCTACA AGGCAGGTAG CCTACGGACA 
CGTGTGAGCC GCTGTGCAGT CTGCATGAAG CGCACGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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col4a5 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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