LOC111086217 cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC111086217
Entrez Gene ID	111086217
Full Name	ATP-dependent RNA helicase DEAH12, chloroplastic-like
Gene Type	protein-coding
Organism	Limulus polyphemus(Atlantic horseshoe crab)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022388167.1	XP_022243875.1	LOW QUALITY PROTEIN: ATP-dependent RNA helicase DEAH12, chloroplastic-like

Pathways from BioSystems
Gene Ontology

The following LOC111086217 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC111086217 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLi111276	XM_022388167.1 Latest version!	Limulus polyphemus ATP-dependent RNA helicase DEAH12, chloroplastic-like (LOC111086217), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC111086217 ( XM_022388167.1 ) cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab) -> XP_022243875.1 Limulus polyphemus ATP-dependent RNA helicase DEAH12, chloroplastic-like (LOC111086217), mRNA.

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CloneID	OLi111276
Clone ID Related Accession (Same CDS sequence)	XM_022388167.1
Accession Version	XM_022388167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504022400000
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Product	LOW QUALITY PROTEIN: ATP-dependent RNA helicase DEAH12, chloroplastic-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013666539.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Limulus polyphemus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAAAAAA ATTCCACCAA ACAAAACCCT TATGTAAGAT CAAGCAACAA TGAACATCAC 
AAGTTTTCTT CTGAAAGGCT TGAGAAATAT GTCTCCTCTA ATATTGTAGT TTCTAAAACC 
TCTCAAGAGT TTCACAGAAT TCAGCAAAAT ATTGTCTCTC ACAATCAGCA TGAAAGTGAA 
AGAAGGTCAT GTGGTGTGGG TTCTCAGCTT CATCATAGTG GATATAAACA TCCAAGAAGT 
GTTTTTCATC AAAAGGTATT GAACTCTAGT GAATTAACAA CAAACAGCCA AGGTAAACAT 
GGGAGGAAAC AACCAACTCA TACAAGGTTT AAAGAGGAAT TGCTATTTTC CTTGAAATCA 
GCTAATGAAA CTTTAGCTGC CAGTAATGAT GTGACTGATT CAAATTCAGA TTCTGAAATA 
AGTGACCTTC AGATACAAGA AAATGTTTGT GAACTATCTG TCTACAACTC AAAAGTAGAA 
CATTCTGAAA TTTCTGATAG TGAGTCAAGT CTAGACACAG TACATGGTGA GAACCATGGC 
AACACTCAGG GCAGTGAAAT ACGTGTTCAG GGGTTCATTA GTGTATTCTG GGATATGAAA 
GCCTGTCCAG TTCCTACTTC AAAATCATTT GTAGATATTA TGAACAAGGT ACGAGAGACC 
CTTTATGAAG GGCGAACAGA ATTTGAAATA AAAATTGCAA TTGATTTCAA TGAGGTTAAT 
GAAAAGGGTC GTTTATTACA AGAATTGCAT GATGCTAAGG TGACAGTTTA TCATATAGCA 
AGCAAAAACC CCTTCAAATA TAGCCTGAGA AACTTTGGTA AATTTTGTAG TGAATGTAAG 
AAACAAGAGG CATCACGACT GGTGGTTTTC ACTGGGAATT CAGTTTTTGC ATCTGATTTG 
TGCAATCTTA GATATGATCT GGGTCTAAAT GTTATTTTGA TACACAATAG AATAATTGAA 
GAGTTTGTTT TAGGTATTCC AAATAAATGT GTTTTATATG ATGAATTTGT GAAGGATCTT 
CCAGATAGAG AAGGTACAGC TGAAAATAAA GTTATTGTGC ACCTTGAAAA TCTGCCCAGC 
AATGAACCAA ATGAATGTGT GAAGAAAAAG CTTGATGAGT TATTGGAGAG TTCTGATGGA 
ATCTTAAAGA ATGTATCGGT GTCTGAGGGT TATGTATACT TCAGCAACAC AACATCTGCA 
AACAGACTAG TGAAATATTT AAATGGATAT AAGTTACTTG ATAATGAAAT AAAAGCAACA 
TTGGTATCAA AACCAAATGT ATTTGCAACT ATGGTAAAGC AAACTAGTAA AGTAGGAACA 
GAAAACAAAG GAGCAATGTG TAAGAGAGAT AAACAAAACC AATTTAAAAC AAACAAAACT 
AAAATTAAGC CCAGAACTAC TGAAACCACT GTGGTTCTTG AATCTACAGA ATGCATCACT 
GATAAGAATT TTTGGATTCT TCGTTTAAAT CACATTTTTA AGCATGAAAT TATATCGTTG 
GAACTAAAAG AACTCACCTC AAATAGAGGA GTTATTCTTT TTTGTAGATT TGCAAGCATG 
GAAGAAGCAG AAAAAGCAGT ATCACTCTTG AATACATCTG GAAAAGAAAC AATAAACCTG 
GAAGTTAAAG ATACCATAAA GAAAAAAGAT GAAATGTCCT ATTATCCAAA CAAAGGTCTT 
AAATTGCAGT TTGATGAATG TTCTCAGTTT ATTCAAAGTA AAGGAAACGA GATCCTGCAA 
AATATCGAGG AAAAGCTGCA AGAAGCCAGA AAAGAATATA AAGACAGAGA GGTTTATTTA 
TGTAATAGAA AACAATCAAA GAACATCAAA AATAATATGA AACTGACTGA AGAACTTTGT 
GCACTTGAAG TAAAAGTGAA CCAGTTAGTA GAACAAAAGG AGGAGTTTTC TAGTTATATT 
AAAGCAAAGA TAGAGGAAAT TTCCTCTTTT GAAGTAGCTT CGAAAGCTAA TGTTGAAATT 
ACCATAAAAA ATGTTAAAAG AGATTTTGGA AGAGAAATTA ATTGTGTCAC TGAAGGCTTG 
CCAATATATG GTAAAAAAAG TGAATTACTA AGGATGATAA AAAAAGAACA GGTTAGTATA 
CTTTTGGCAG AAACAGGGTC TGGAAAAAGC ACTCAGGTTG TTCAGTATTT ATGGCGATCT 
GGTTTGATTG CAGACAAACA GATTATTTGT ACACAACCAA GAAAAATTGC AGCTATATCT 
CTAGCAAAGC ATGTGAGTGG ACAGCTAGGT TTCCCTCTAG GGAATATTGT GGGATACCAA 
GTGGGAATGA GTATCAAAAA GGATAAGCAT ACGAAAATCA CATTCATGAC AGACCACACA 
CTTTTAAATG TTGCAGTCAA AGATCAAACT TTTTCAGATT GTGGATGCAT TATAATTGAT 
GAGGCTCATG AACGCAGTAT TTTTACTGAC GTTTTGCTGG GTGTAATTAA GCACTGTCTT 
TCACACAGAC CAGACCTAAA AATTATCATT ACCTCTGCTA CCATTGATCC AGCCNATTTT 
ATGACATTCT TTGGTATTGA AGAAAAAGCA TTATTAAACA TACCTGGTCG GACGTTTCCT 
GTAGAAGTTG TCTGGAAGGA CTGTGATGTT AAGGAAGGTT GGGATTATCT ATCTGATTCC 
ATAGACATGG CCATTCAGGT ACACCAAAAA CAACCACCAG GTGACATTCT CACTTTTTTA 
ACTTCACCTG CTGAAACAGA AAAAGCCGTA GATTTGTTTC AAGAAAAAAT GAAACAAAAA 
AGTCCAGGTA GCTTTATTTG TTTACTAGTT CATGGAAAGC AAGATATTCA AGAACAAGAA 
AAAGTATTTA AACCAGCTCC AGCAGGGAAA CGAAAGATAA TTTTTGCCAC CAACAGTGCT 
GAAACATCAG TTACTATCAA TGGAGTCGTC TATGTAATCG ATTCAGGAAT GGTGAAAGAG 
CTGCAGTATT CTCCTCAGAA GAATTCTAAT GCTCTTGTTG TCTGTTTTGA ATCAAAAAGT 
GCGGCAGAAC AACGGAAAGG CCGTGCAGGT AGAACCCAAC CTGGAAAATG TTTTCGAATG 
TTTAGCAAAA ACAACTTTGA GAACATGAAG CATTGTACTG TACCAGAAAT CTTGAGAACA 
AATGTTTCTC AGGCTTTGTT GAAAATCATG GAAGTTATTC CTGGCAATCC CTTAATGTTT 
GATTTTGTTG AATCTCCCTC AACAGAAGTT CTCAGAAAAG CATATGACCT TCTAGAACAT 
TTGGATGCAG TGAAAGCTAA TGAACTAACA TGTCTTGGAA GAAAGATGGC TAAACTTTCA 
TTAGAACCTC GTTTGTCTAG GTTGGTTATT CAAGGAATCT TTGGTGGATT TGGTCTAGAT 
GCAATTGTAG TTGCATCTAC TGCAACTGTT TCTCATACTA TTTTCTTTCG CATAGAAGAT 
AACAAAAATG AAGCTGACCT GAAAAAGATT CAGTTTTGTC AAACTGAAGG GGATCTTTTT 
ACTTACCTTA GTGTTTATAA AGAATGGTTT GCTGTTCCAA AAAAATTCAG GCCTAGTTGG 
TGTGTTACCA ATTGCATCAA CAATAAGGCA TTGAAGATTG CTCATGAAGT AGTAGAAGAC 
TGTGTTAATA CTGTTAAGAA AGAACTGGGA ATTTCTGTGC AAAGAAGCTT CTGCAGTGAC 
GACAACCATA AAGAAAGATT ACAAAATCTC ATTCTTGAAA GTTTTTCAGA TAACCTTTGT 
ATATTTTCAG GCCACCCAAA ATTGGGATAT TTTACAACAT CAGGAGATCA GATTCATGTT 
CACCCATCTG CATACATTCG TTGTCTAGAC TGTGATATGC CAAAGTTTTT TGTCTATGGT 
ACTCGTCTCA AGACATATTT AGAATATGCT CTGTGCATCT CCACTGTTTC TGAAGAGATG 
GTTTCTAAAC TGTTTCAAAG AAAAAATCTC AACTTTGATC TGGATTCATT TAAACAGCTT 
ACTGTCCAAC CAGTAAAACT TGGACCCATA GGAAAAACTC TTCTACTAAG AGAAATTCTT 
GGAAATCAGT TAAGCAAGAT GAATATGTTT AAAAATGAAA TTAAAAGCAT TACAAATACT 
GAAGTAATGC ACATAGATTG GAATGTGAAT AAAGGAGCTG TGTATGTGTA CATAGCTCAA 
CAGTTTACTT CTGTTATAGA GGAAGTATTC TACAATTTCA TTAAAGAACC TAAACAAAAG 
ATGCTAGAAG AGGACAAAGA AGTTAATTTT GCAGAACTAC TAAACTACCC CAAGGGTGTA 
GGACCCACGA TAATACTAGG CAGTGGTTCA GAAGTGAAAA ACATTTTAAT GCCTGGTGAC 
TTTAGAACCA TTATTCTTAA CATTAATCAA AACACTAACA GTGTTAAGAT TCTTGATACA 
CTAAAATCGT TTGGAAAAGT GAAATCACAC AGAGAATTCA GAGATAAGAA TAAAATTCAT 
GTGACATTTT TCGATCCCGT CAGTGCTCAA AGATCTCTTC AGTCCCTGAA CAATAATACA 
GATATAAGAA ATGTCAGACC ATTTATCCCT AACATGTGTG AAACCAGACA TCGAGCAGTG 
ATGGAGGTAA CTTGGTGTAG GAGGCCTTGT AAGGGTTATG GTTTTGTAAA CTTCAAATCT 
CCAGACGAGT TCCTCTGTGT TGCTGATCTG TTTTCTCATT TCAGGAACTT TGCTGGTGTG 
ACAGTCAGGA GAGACAGAAA AAAAGCTAAC ACGTTGTACC TTCGAAATAT TTCATCTTTT 
GCTACGCAAC AAACAGTACA GAAATCTCTG GAAGGAGTTC TTTATGGATT AGATATCAAT 
GTTGCTGTGA TTCGGGAAAA CCCTTTTGTG TCTAGTTCAA CTGAGTGTGA TAATTTGGAA 
AGAGAGTTGA CTTCATTGAT TTCGAAATTC TGCAGTGGGC ATAATTTGGT TGTCAAGTTG 
CAAAAACCGA GGGAAAGTGA CGTGATGTGG AAAGCCAGAG TGTTTTTCAC TCTTCCCAAA 
GATGGGGAAC ATGCTGCACA TTGTCTGAAT GGAGCTAAGA TTGGATCACA AAATGCTCCA 
TTGTCTCTTA AAATAAAATT TAATTGTATA ATGTTTTGTT GTTTAAAGTC ATATAATGCT 
GTAAAGGCAG TTTTTGAAGA ATCTATAACT GATTTCAAGA GGATTGGTGC TTGTAATGAC 
AATTTCAGCA TTGAAATAAC AAAGATAATG GAAATCAAAG TAAAGGTAGT GATCAGAACA 
GATAACTTGC ATGACCTGAA GAGAGCTCAA AAGTTCTTTT TGGAAATCCT TCAAGGTGAT 
CAGATACATT GTAAAGGAAG ACCACTTTTG GAGAAACTGT TTGCTACACA TTCACAAAAA 
GTTATGGAAA CTATAGAAAA AGACACAAAC ACGTATATCC ACTTGGATCA CAAATCAAAA 
ATTATTTCCA TTTTTGGATC TGTAGGAGCA TGCACCTTAG CTAAATGTGA ATTAAATAAA 
TGGTTGAACA TGATGGAAGG CTGTGAAATC AGTGTGTTTA CATTAAGGGA CCAGAAAGGA 
CCGAAAGGTT TACTAAAGGA ACTGGTGAAA ACTTATGGAT ACAGACTCAC TGATTTTATA 
GATAAATGTG GTCTTGAATC TGCAGAGATT GACCTCCAGA AACATCTACT TGTGGTAACT 
GGAGCTTCAT ATGCCATAGA AAAAGCAGCA TCAGAATTTG AACATGTGAA GGAAAGGTTA 
CTTGTGGCTC AGGAAAACCA TCCAGATGTG GATGATGAAG AATGTCCTGT GTGCTTCATC 
ACAGTAGACC CAACAGCTGG TTATCGCTTG GAATATTGTG GCCATCTTCA TTGTAAAGAA 
TGTCTTCTGT TACAAATAGA AGCAAAACAA ATTCCTTTAC TTTGTTCAGC AGAGGGCTGT 
GGACAATATT TTGTAATGGA AGACCTTAAA AAAATTCTTG AGGGCAATGA AAAGCTGAGA 
AAAGATCTTA TTGAAGAATC CTTAAAAAAG TTTGTTGAAA TGAACAAGGA ATATCGTTAT 
TGCCCATCTT TAGATTGTCC AATGGTTTAC CATATTACAC CCCCTGGTGG TTCATTTATC 
TGCGGATTTT GTGAAAATGA TATATGTTCA CATTGTCACA CACACTACCA CAGTGGTATT 
ACATGTGAGT TAAATAAAAC TTGTGCAGAA GATGAAGATT ACTCTCTAGT GGTTTGGATG 
GCGAAAGACG CTTCGAACAG AAAACTGTGT CCATCGTGTA AGGCTCCCAT CGAAAAGAAC 
GAGGGGTGTA ACCACATGTT TTGTAGTAAA TGTCAAACTC ACTTTTGCTG GGTGTGCCTA 
GAGAAGTTTC GTAACGTAAC TGATTTTAAT AAGCACCAAC CATATTGCCT ACGAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022243875.1
CDS	889..7248
Translation	MEKNSTKQNPYVRSSNNEHHKFSSERLEKYVSSNIVVSKTSQEFHRIQQNIVSHNQHESERRSCGVGSQLHHSGYKHPRSVFHQKVLNSSELTTNSQGKHGRKQPTHTRFKEELLFSLKSANETLAASNDVTDSNSDSEISDLQIQENVCELSVYNSKVEHSEISDSESSLDTVHGENHGNTQGSEIRVQGFISVFWDMKACPVPTSKSFVDIMNKVRETLYEGRTEFEIKIAIDFNEVNEKGRLLQELHDAKVTVYHIASKNPFKYSLRNFGKFCSECKKQEASRLVVFTGNSVFASDLCNLRYDLGLNVILIHNRIIEEFVLGIPNKCVLYDEFVKDLPDREGTAENKVIVHLENLPSNEPNECVKKKLDELLESSDGILKNVSVSEGYVYFSNTTSANRLVKYLNGYKLLDNEIKATLVSKPNVFATMVKQTSKVGTENKGAMCKRDKQNQFKTNKTKIKPRTTETTVVLESTECITDKNFWILRLNHIFKHEIISLELKELTSNRGVILFCRFASMEEAEKAVSLLNTSGKETINLEVKDTIKKKDEMSYYPNKGLKLQFDECSQFIQSKGNEILQNIEEKLQEARKEYKDREVYLCNRKQSKNIKNNMKLTEELCALEVKVNQLVEQKEEFSSYIKAKIEEISSFEVASKANVEITIKNVKRDFGREINCVTEGLPIYGKKSELLRMIKKEQVSILLAETGSGKSTQVVQYLWRSGLIADKQIICTQPRKIAAISLAKHVSGQLGFPLGNIVGYQVGMSIKKDKHTKITFMTDHTLLNVAVKDQTFSDCGCIIIDEAHERSIFTDVLLGVIKHCLSHRPDLKIIITSATIDPAnullFMTFFGIEEKALLNIPGRTFPVEVVWKDCDVKEGWDYLSDSIDMAIQVHQKQPPGDILTFLTSPAETEKAVDLFQEKMKQKSPGSFICLLVHGKQDIQEQEKVFKPAPAGKRKIIFATNSAETSVTINGVVYVIDSGMVKELQYSPQKNSNALVVCFESKSAAEQRKGRAGRTQPGKCFRMFSKNNFENMKHCTVPEILRTNVSQALLKIMEVIPGNPLMFDFVESPSTEVLRKAYDLLEHLDAVKANELTCLGRKMAKLSLEPRLSRLVIQGIFGGFGLDAIVVASTATVSHTIFFRIEDNKNEADLKKIQFCQTEGDLFTYLSVYKEWFAVPKKFRPSWCVTNCINNKALKIAHEVVEDCVNTVKKELGISVQRSFCSDDNHKERLQNLILESFSDNLCIFSGHPKLGYFTTSGDQIHVHPSAYIRCLDCDMPKFFVYGTRLKTYLEYALCISTVSEEMVSKLFQRKNLNFDLDSFKQLTVQPVKLGPIGKTLLLREILGNQLSKMNMFKNEIKSITNTEVMHIDWNVNKGAVYVYIAQQFTSVIEEVFYNFIKEPKQKMLEEDKEVNFAELLNYPKGVGPTIILGSGSEVKNILMPGDFRTIILNINQNTNSVKILDTLKSFGKVKSHREFRDKNKIHVTFFDPVSAQRSLQSLNNNTDIRNVRPFIPNMCETRHRAVMEVTWCRRPCKGYGFVNFKSPDEFLCVADLFSHFRNFAGVTVRRDRKKANTLYLRNISSFATQQTVQKSLEGVLYGLDINVAVIRENPFVSSSTECDNLERELTSLISKFCSGHNLVVKLQKPRESDVMWKARVFFTLPKDGEHAAHCLNGAKIGSQNAPLSLKIKFNCIMFCCLKSYNAVKAVFEESITDFKRIGACNDNFSIEITKIMEIKVKVVIRTDNLHDLKRAQKFFLEILQGDQIHCKGRPLLEKLFATHSQKVMETIEKDTNTYIHLDHKSKIISIFGSVGACTLAKCELNKWLNMMEGCEISVFTLRDQKGPKGLLKELVKTYGYRLTDFIDKCGLESAEIDLQKHLLVVTGASYAIEKAASEFEHVKERLLVAQENHPDVDDEECPVCFITVDPTAGYRLEYCGHLHCKECLLLQIEAKQIPLLCSAEGCGQYFVMEDLKKILEGNEKLRKDLIEESLKKFVEMNKEYRYCPSLDCPMVYHITPPGGSFICGFCENDICSHCHTHYHSGITCELNKTCAEDEDYSLVVWMAKDASNRKLCPSCKAPIEKNEGCNHMFCSKCQTHFCWVCLEKFRNVTDFNKHQPYCLRN

Target ORF information:

RefSeq Version	XM_022388167.1
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Definition	Limulus polyphemus ATP-dependent RNA helicase DEAH12, chloroplastic-like (LOC111086217), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022388167.1

ORF Insert Sequence:

ATGGAAAAAA ATTCCACCAA ACAAAACCCT TATGTAAGAT CAAGCAACAA TGAACATCAC 
AAGTTTTCTT CTGAAAGGCT TGAGAAATAT GTCTCCTCTA ATATTGTAGT TTCTAAAACC 
TCTCAAGAGT TTCACAGAAT TCAGCAAAAT ATTGTCTCTC ACAATCAGCA TGAAAGTGAA 
AGAAGGTCAT GTGGTGTGGG TTCTCAGCTT CATCATAGTG GATATAAACA TCCAAGAAGT 
GTTTTTCATC AAAAGGTATT GAACTCTAGT GAATTAACAA CAAACAGCCA AGGTAAACAT 
GGGAGGAAAC AACCAACTCA TACAAGGTTT AAAGAGGAAT TGCTATTTTC CTTGAAATCA 
GCTAATGAAA CTTTAGCTGC CAGTAATGAT GTGACTGATT CAAATTCAGA TTCTGAAATA 
AGTGACCTTC AGATACAAGA AAATGTTTGT GAACTATCTG TCTACAACTC AAAAGTAGAA 
CATTCTGAAA TTTCTGATAG TGAGTCAAGT CTAGACACAG TACATGGTGA GAACCATGGC 
AACACTCAGG GCAGTGAAAT ACGTGTTCAG GGGTTCATTA GTGTATTCTG GGATATGAAA 
GCCTGTCCAG TTCCTACTTC AAAATCATTT GTAGATATTA TGAACAAGGT ACGAGAGACC 
CTTTATGAAG GGCGAACAGA ATTTGAAATA AAAATTGCAA TTGATTTCAA TGAGGTTAAT 
GAAAAGGGTC GTTTATTACA AGAATTGCAT GATGCTAAGG TGACAGTTTA TCATATAGCA 
AGCAAAAACC CCTTCAAATA TAGCCTGAGA AACTTTGGTA AATTTTGTAG TGAATGTAAG 
AAACAAGAGG CATCACGACT GGTGGTTTTC ACTGGGAATT CAGTTTTTGC ATCTGATTTG 
TGCAATCTTA GATATGATCT GGGTCTAAAT GTTATTTTGA TACACAATAG AATAATTGAA 
GAGTTTGTTT TAGGTATTCC AAATAAATGT GTTTTATATG ATGAATTTGT GAAGGATCTT 
CCAGATAGAG AAGGTACAGC TGAAAATAAA GTTATTGTGC ACCTTGAAAA TCTGCCCAGC 
AATGAACCAA ATGAATGTGT GAAGAAAAAG CTTGATGAGT TATTGGAGAG TTCTGATGGA 
ATCTTAAAGA ATGTATCGGT GTCTGAGGGT TATGTATACT TCAGCAACAC AACATCTGCA 
AACAGACTAG TGAAATATTT AAATGGATAT AAGTTACTTG ATAATGAAAT AAAAGCAACA 
TTGGTATCAA AACCAAATGT ATTTGCAACT ATGGTAAAGC AAACTAGTAA AGTAGGAACA 
GAAAACAAAG GAGCAATGTG TAAGAGAGAT AAACAAAACC AATTTAAAAC AAACAAAACT 
AAAATTAAGC CCAGAACTAC TGAAACCACT GTGGTTCTTG AATCTACAGA ATGCATCACT 
GATAAGAATT TTTGGATTCT TCGTTTAAAT CACATTTTTA AGCATGAAAT TATATCGTTG 
GAACTAAAAG AACTCACCTC AAATAGAGGA GTTATTCTTT TTTGTAGATT TGCAAGCATG 
GAAGAAGCAG AAAAAGCAGT ATCACTCTTG AATACATCTG GAAAAGAAAC AATAAACCTG 
GAAGTTAAAG ATACCATAAA GAAAAAAGAT GAAATGTCCT ATTATCCAAA CAAAGGTCTT 
AAATTGCAGT TTGATGAATG TTCTCAGTTT ATTCAAAGTA AAGGAAACGA GATCCTGCAA 
AATATCGAGG AAAAGCTGCA AGAAGCCAGA AAAGAATATA AAGACAGAGA GGTTTATTTA 
TGTAATAGAA AACAATCAAA GAACATCAAA AATAATATGA AACTGACTGA AGAACTTTGT 
GCACTTGAAG TAAAAGTGAA CCAGTTAGTA GAACAAAAGG AGGAGTTTTC TAGTTATATT 
AAAGCAAAGA TAGAGGAAAT TTCCTCTTTT GAAGTAGCTT CGAAAGCTAA TGTTGAAATT 
ACCATAAAAA ATGTTAAAAG AGATTTTGGA AGAGAAATTA ATTGTGTCAC TGAAGGCTTG 
CCAATATATG GTAAAAAAAG TGAATTACTA AGGATGATAA AAAAAGAACA GGTTAGTATA 
CTTTTGGCAG AAACAGGGTC TGGAAAAAGC ACTCAGGTTG TTCAGTATTT ATGGCGATCT 
GGTTTGATTG CAGACAAACA GATTATTTGT ACACAACCAA GAAAAATTGC AGCTATATCT 
CTAGCAAAGC ATGTGAGTGG ACAGCTAGGT TTCCCTCTAG GGAATATTGT GGGATACCAA 
GTGGGAATGA GTATCAAAAA GGATAAGCAT ACGAAAATCA CATTCATGAC AGACCACACA 
CTTTTAAATG TTGCAGTCAA AGATCAAACT TTTTCAGATT GTGGATGCAT TATAATTGAT 
GAGGCTCATG AACGCAGTAT TTTTACTGAC GTTTTGCTGG GTGTAATTAA GCACTGTCTT 
TCACACAGAC CAGACCTAAA AATTATCATT ACCTCTGCTA CCATTGATCC AGCCNATTTT 
ATGACATTCT TTGGTATTGA AGAAAAAGCA TTATTAAACA TACCTGGTCG GACGTTTCCT 
GTAGAAGTTG TCTGGAAGGA CTGTGATGTT AAGGAAGGTT GGGATTATCT ATCTGATTCC 
ATAGACATGG CCATTCAGGT ACACCAAAAA CAACCACCAG GTGACATTCT CACTTTTTTA 
ACTTCACCTG CTGAAACAGA AAAAGCCGTA GATTTGTTTC AAGAAAAAAT GAAACAAAAA 
AGTCCAGGTA GCTTTATTTG TTTACTAGTT CATGGAAAGC AAGATATTCA AGAACAAGAA 
AAAGTATTTA AACCAGCTCC AGCAGGGAAA CGAAAGATAA TTTTTGCCAC CAACAGTGCT 
GAAACATCAG TTACTATCAA TGGAGTCGTC TATGTAATCG ATTCAGGAAT GGTGAAAGAG 
CTGCAGTATT CTCCTCAGAA GAATTCTAAT GCTCTTGTTG TCTGTTTTGA ATCAAAAAGT 
GCGGCAGAAC AACGGAAAGG CCGTGCAGGT AGAACCCAAC CTGGAAAATG TTTTCGAATG 
TTTAGCAAAA ACAACTTTGA GAACATGAAG CATTGTACTG TACCAGAAAT CTTGAGAACA 
AATGTTTCTC AGGCTTTGTT GAAAATCATG GAAGTTATTC CTGGCAATCC CTTAATGTTT 
GATTTTGTTG AATCTCCCTC AACAGAAGTT CTCAGAAAAG CATATGACCT TCTAGAACAT 
TTGGATGCAG TGAAAGCTAA TGAACTAACA TGTCTTGGAA GAAAGATGGC TAAACTTTCA 
TTAGAACCTC GTTTGTCTAG GTTGGTTATT CAAGGAATCT TTGGTGGATT TGGTCTAGAT 
GCAATTGTAG TTGCATCTAC TGCAACTGTT TCTCATACTA TTTTCTTTCG CATAGAAGAT 
AACAAAAATG AAGCTGACCT GAAAAAGATT CAGTTTTGTC AAACTGAAGG GGATCTTTTT 
ACTTACCTTA GTGTTTATAA AGAATGGTTT GCTGTTCCAA AAAAATTCAG GCCTAGTTGG 
TGTGTTACCA ATTGCATCAA CAATAAGGCA TTGAAGATTG CTCATGAAGT AGTAGAAGAC 
TGTGTTAATA CTGTTAAGAA AGAACTGGGA ATTTCTGTGC AAAGAAGCTT CTGCAGTGAC 
GACAACCATA AAGAAAGATT ACAAAATCTC ATTCTTGAAA GTTTTTCAGA TAACCTTTGT 
ATATTTTCAG GCCACCCAAA ATTGGGATAT TTTACAACAT CAGGAGATCA GATTCATGTT 
CACCCATCTG CATACATTCG TTGTCTAGAC TGTGATATGC CAAAGTTTTT TGTCTATGGT 
ACTCGTCTCA AGACATATTT AGAATATGCT CTGTGCATCT CCACTGTTTC TGAAGAGATG 
GTTTCTAAAC TGTTTCAAAG AAAAAATCTC AACTTTGATC TGGATTCATT TAAACAGCTT 
ACTGTCCAAC CAGTAAAACT TGGACCCATA GGAAAAACTC TTCTACTAAG AGAAATTCTT 
GGAAATCAGT TAAGCAAGAT GAATATGTTT AAAAATGAAA TTAAAAGCAT TACAAATACT 
GAAGTAATGC ACATAGATTG GAATGTGAAT AAAGGAGCTG TGTATGTGTA CATAGCTCAA 
CAGTTTACTT CTGTTATAGA GGAAGTATTC TACAATTTCA TTAAAGAACC TAAACAAAAG 
ATGCTAGAAG AGGACAAAGA AGTTAATTTT GCAGAACTAC TAAACTACCC CAAGGGTGTA 
GGACCCACGA TAATACTAGG CAGTGGTTCA GAAGTGAAAA ACATTTTAAT GCCTGGTGAC 
TTTAGAACCA TTATTCTTAA CATTAATCAA AACACTAACA GTGTTAAGAT TCTTGATACA 
CTAAAATCGT TTGGAAAAGT GAAATCACAC AGAGAATTCA GAGATAAGAA TAAAATTCAT 
GTGACATTTT TCGATCCCGT CAGTGCTCAA AGATCTCTTC AGTCCCTGAA CAATAATACA 
GATATAAGAA ATGTCAGACC ATTTATCCCT AACATGTGTG AAACCAGACA TCGAGCAGTG 
ATGGAGGTAA CTTGGTGTAG GAGGCCTTGT AAGGGTTATG GTTTTGTAAA CTTCAAATCT 
CCAGACGAGT TCCTCTGTGT TGCTGATCTG TTTTCTCATT TCAGGAACTT TGCTGGTGTG 
ACAGTCAGGA GAGACAGAAA AAAAGCTAAC ACGTTGTACC TTCGAAATAT TTCATCTTTT 
GCTACGCAAC AAACAGTACA GAAATCTCTG GAAGGAGTTC TTTATGGATT AGATATCAAT 
GTTGCTGTGA TTCGGGAAAA CCCTTTTGTG TCTAGTTCAA CTGAGTGTGA TAATTTGGAA 
AGAGAGTTGA CTTCATTGAT TTCGAAATTC TGCAGTGGGC ATAATTTGGT TGTCAAGTTG 
CAAAAACCGA GGGAAAGTGA CGTGATGTGG AAAGCCAGAG TGTTTTTCAC TCTTCCCAAA 
GATGGGGAAC ATGCTGCACA TTGTCTGAAT GGAGCTAAGA TTGGATCACA AAATGCTCCA 
TTGTCTCTTA AAATAAAATT TAATTGTATA ATGTTTTGTT GTTTAAAGTC ATATAATGCT 
GTAAAGGCAG TTTTTGAAGA ATCTATAACT GATTTCAAGA GGATTGGTGC TTGTAATGAC 
AATTTCAGCA TTGAAATAAC AAAGATAATG GAAATCAAAG TAAAGGTAGT GATCAGAACA 
GATAACTTGC ATGACCTGAA GAGAGCTCAA AAGTTCTTTT TGGAAATCCT TCAAGGTGAT 
CAGATACATT GTAAAGGAAG ACCACTTTTG GAGAAACTGT TTGCTACACA TTCACAAAAA 
GTTATGGAAA CTATAGAAAA AGACACAAAC ACGTATATCC ACTTGGATCA CAAATCAAAA 
ATTATTTCCA TTTTTGGATC TGTAGGAGCA TGCACCTTAG CTAAATGTGA ATTAAATAAA 
TGGTTGAACA TGATGGAAGG CTGTGAAATC AGTGTGTTTA CATTAAGGGA CCAGAAAGGA 
CCGAAAGGTT TACTAAAGGA ACTGGTGAAA ACTTATGGAT ACAGACTCAC TGATTTTATA 
GATAAATGTG GTCTTGAATC TGCAGAGATT GACCTCCAGA AACATCTACT TGTGGTAACT 
GGAGCTTCAT ATGCCATAGA AAAAGCAGCA TCAGAATTTG AACATGTGAA GGAAAGGTTA 
CTTGTGGCTC AGGAAAACCA TCCAGATGTG GATGATGAAG AATGTCCTGT GTGCTTCATC 
ACAGTAGACC CAACAGCTGG TTATCGCTTG GAATATTGTG GCCATCTTCA TTGTAAAGAA 
TGTCTTCTGT TACAAATAGA AGCAAAACAA ATTCCTTTAC TTTGTTCAGC AGAGGGCTGT 
GGACAATATT TTGTAATGGA AGACCTTAAA AAAATTCTTG AGGGCAATGA AAAGCTGAGA 
AAAGATCTTA TTGAAGAATC CTTAAAAAAG TTTGTTGAAA TGAACAAGGA ATATCGTTAT 
TGCCCATCTT TAGATTGTCC AATGGTTTAC CATATTACAC CCCCTGGTGG TTCATTTATC 
TGCGGATTTT GTGAAAATGA TATATGTTCA CATTGTCACA CACACTACCA CAGTGGTATT 
ACATGTGAGT TAAATAAAAC TTGTGCAGAA GATGAAGATT ACTCTCTAGT GGTTTGGATG 
GCGAAAGACG CTTCGAACAG AAAACTGTGT CCATCGTGTA AGGCTCCCAT CGAAAAGAAC 
GAGGGGTGTA ACCACATGTT TTGTAGTAAA TGTCAAACTC ACTTTTGCTG GGTGTGCCTA 
GAGAAGTTTC GTAACGTAAC TGATTTTAAT AAGCACCAAC CATATTGCCT ACGAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC111086217 cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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