LOC106468631 cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106468631
Entrez Gene ID	106468631
Full Name	protein jagged-1-like
Gene Type	protein-coding
Organism	Limulus polyphemus(Atlantic horseshoe crab)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022396976.1	XP_022252684.1	protein jagged-1-like
XM_013929059.1	XP_013784513.1	protein jagged-1-like

Pathways from BioSystems
Gene Ontology

The following LOC106468631 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106468631 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLi12541	XM_013929059.1 Latest version!	Limulus polyphemus protein jagged-1-like (LOC106468631), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OLi99962	XM_022396976.1 Latest version!	Limulus polyphemus protein jagged-1-like (LOC106468631), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106468631 ( XM_013929059.1 ) cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab) -> XP_013784513.1 Limulus polyphemus protein jagged-1-like (LOC106468631), mRNA.

Price & Availability↑

CloneID	OLi12541
Clone ID Related Accession (Same CDS sequence)	XM_013929059.1
Accession Version	XM_013929059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441641600000
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Product	protein jagged-1-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013668699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Limulus polyphemus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 12% of CDS bases ##RefSeq-Attributes-END##

ATGCGCAGGG CGGGTCCAGC GCCTCACGAA ACGTTTGGCT CCTCGTATCA GTGTACCTCT 
GTTCTTCAAG TTACTCTTTC CATCCTTCAC GTGCTGGACG CGTCCGGGTC GTTTGAATTA 
CAGATTTTAG GAATACAGAA CTCTAGGGGT GAACTACTGA ACAGAGCTTG CTGTGGGGGT 
GAGGCGAGAA AAGAAGAGGA GATCTGTCCT AGGGAATGTA ACACTTACTT TCGCCTGTGT 
CTGAAACAGT ATCAATCACA GATTACAGCC AAGGGATCGT GTACATTCGG CAGCACGTCT 
TCACCGGTTG CCGTCGGAAA TTCCTTTTCA GTTCACAACG AGCCGGAAAA AGAAGTGGTA 
CTCAAGCTGC CCTTTGAATT TAGATGGACG AAATCATTCA CGTTAATCCT TGAAGCAGTA 
GACCACAACA ACCGTTCAAA ATCATCTCGT GACGAAGTGA TCGAAAGACA TATGTATTCG 
GGCATTATTA CACCCAGCGC AGAGTGGCAC CAACTATCAC ACCCAGGTAC TACAGCTACT 
ATAAACTATA GAATACGTGT GACGTGCGAC CCTTACTATT ACAATTACAC GTGCACCAAA 
TTTTGTCGAC CGAGGCACGA CCGTTTTGGA CACTACACTT GTAGTAAACA AGGAGACAAG 
ATCTGTTTGG TGGGATGGAA AGGACCTAAC TGTGAAACAG CGGTTTGTAA GGAAGGGTGT 
CACCCGCAAC ATGGAAACTG TGAGAAACCT GGAGAATGCA AAAGGAACCT TCATCGTCCA 
TCACTCCAGC AGCTTGGAAC TTCTCTTACT CCACTGGAAG AAACGGGTAG AAAAAAACAC 
AGTGGAGAGT CCGGAAGACA CACCAGAGAC AGTTCGTCAT CGAATTTAGT GACCTTGGGC 
AGCGGTCAAC TACGTCAATC GAAAGGGTTT GACCTTCTTA TTATTTCACT GGAACCATCA 
GATGCTGACG AATGCGTAAA AGATCCTTGT ATCAACGCTC TCTTGTGTAA GAATGTGGTT 
GGAGATTATA TCTGTGAATG CCAACCTGGA TGGATGGGAA AAAATTGCGA TCACAACATT 
AATGATTGTG TTGGTCAATG TCAAAACGGA GCCACGTGTA TTGATTTGGT AAGCGACTAC 
CACTGCGCCT GCTTCCCAGG ATTTACAGGA AGGAACTGCC AGACCAACAT TGACGACTGT 
GCCAGCAACC CCTGTTTGAA CGGAGGAGAA TGTGTAGACC TTGTGGCTGC TTTCTCTTGC 
ATCTGCCCTG TTGGGTATAA AGGAGCGAGG TGCGAGATCG ATGACGATTT TTGTAATCCC 
AATCCCTGTG AAAATGGAGC GTCCTGTTTC AACATGCATA GGGATTACTA TTGCCATTGT 
TCTGCTGAAT ATCACGGAAA AAACTGCTCC CAACCAAGAC CCAAGTGTAC TTCTCCTCCT 
TGTGAAGGTA AGTTTATTTT CTGTTTTACA AGGAACTGTG GAAAACAGCG ACTTAGCGGT 
TCAAATGTCT GTGGAGATCA TGGCAAGTGT ATCAGTGAAC CACTAGGGGG ATTCACATGC 
GTCTGTGATC CAGGCTATAC TGGCAGATAT TGTCATGAAA ATATAAACGA CTGTGTTTCC 
AACCCTTGCA AACATGGTGG TACCTGTGTG GACAAAATAA ACTCATTCCT ATGTGTTTGT 
AAAGAAGGCT GGGAAGGACC TTTTTGTAAT ACGAATAAGA ACGATTGTGA ACCGAATCCC 
TGTCATAACA ATGGAACATG CATCGACTCA ATCGCGGACT TTATTTGTGA ATGTAGACAT 
GGGTGGAAGG GGAAAACTTG CAACTTTAGA CACAGTCATT GTGCTGTCAA TCCCTGCGCC 
AATGGAGGAA CATGTACAGA TCTAGGAGAC AACTTTGTAT GTCAATGCCC ACGTGGGTTT 
GAAGGCCACA CATGTCAGAT AGCTAATAAT CCCAGTTGTC TCTCCAGTCC ATGCCAAAAT 
GGTGGCACCT GTATAAGCGT AAGCATGGGA TCAGGGTTTT CCTGTGTATG CAAAGAAGGT 
TATGAAGGCA TATTATGTCA AAATAATATT GATGATTGTA ACCCCAACCC TTGCTACAAT 
AATGGAAGAT GTGTGGATGG CATAAACTGG TTTCGATGTG AATGTGCCAA AGGTTTTGCT 
GGACCAGATT GTCGTATTAA CATTGATGAC TGTGCTTCCT CTCCTTGTGC GGAGGGAAGC 
ACCTGCAGAG ATGGTATTGC TGACTTTCAA TGTATCTGTC CACCTGGTCA GACAGGAAGA 
TTTTGTAACA TAGGGGAAAA GATTCATTGT CCTCAGGGAC AGGAGTGTGT CCCTAATTTC 
CACAGTACCT GTTTTATTCC TCCTTGCCCT TCAATAGGAA TTTGTGTGTC CAAGGAAAAT 
TCCACCAAAA GCTCCATGGC TGGATTGATT CCAAACACAA AATGTAGACC AAATTCAGCA 
GAGTTGGGAA ACAACTGTGC TACTGTTACT TTAGTGTTCA ACCGGAGTAA AATACTACAG 
GGACTGACAG TTGAAAGTTT GTGTACACAG ATACGAAGGT TACCCAAGCA GCTGAACCAT 
ACACCAGTCC ATACAGTTTT TCTTCTTTGT GATCAAGTAG AAGGTGTGCC AGATAGCATA 
GAAGTGGCAG TGTCTAGTAA TGGAGATCCT TCCTTTGAAA CCTACAAATA CGTTAGTAAC 
ATTGCAAAAT GGCTGGCAGA CAGAATAGGC CGTAAACGAT CTAACAGTAC ATCCTTAAGT 
GCCGTGATGG AGGTGAAAGT AAAGACCTCT CTTCTTGCTT CCACTCATGG ACCTGGCTAC 
TTGGTCCCAG TGCTGGTGTC CCTCTTAGCT TTACTGCTGC TAGCTTGTTT CATTGGGGTC 
GGACTCTGGT ATTATCGTAA TCATTATCGA AGAAAAGGAT CCTTGGTCCA CCATCGTCAG 
CCGTTACGAC ACTCAATTAG CAGTAAATTA GAGCAGATTG AAGAAGAAGA AAAGTCTAAT 
AACCAGAATG AAGAAAATCT GCGACGATAC CACAATCCTC TTCACACAAG TAATGATTTG 
AAGGAAAAGC TGAGCTCGCC AAATCTGAGC GATTCCACAG AGATGACACA AGTAGACAAC 
ATTAATGACT CCTCCCACAT TCCTCAAAAA ATATACAAAC CCCAATCTGT AGAATCACAA 
AAAAACATCA CTCCCATAAA TATTACAGAA AAAGCCTTGG AGAAAGACAA CAATCTTAAA 
TCTCATCAAA GAAGGCCATC TGGAAAAGAA GTCATAGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013784513.1
CDS	1..3282
Translation	MRRAGPAPHETFGSSYQCTSVLQVTLSILHVLDASGSFELQILGIQNSRGELLNRACCGGEARKEEEICPRECNTYFRLCLKQYQSQITAKGSCTFGSTSSPVAVGNSFSVHNEPEKEVVLKLPFEFRWTKSFTLILEAVDHNNRSKSSRDEVIERHMYSGIITPSAEWHQLSHPGTTATINYRIRVTCDPYYYNYTCTKFCRPRHDRFGHYTCSKQGDKICLVGWKGPNCETAVCKEGCHPQHGNCEKPGECKRNLHRPSLQQLGTSLTPLEETGRKKHSGESGRHTRDSSSSNLVTLGSGQLRQSKGFDLLIISLEPSDADECVKDPCINALLCKNVVGDYICECQPGWMGKNCDHNINDCVGQCQNGATCIDLVSDYHCACFPGFTGRNCQTNIDDCASNPCLNGGECVDLVAAFSCICPVGYKGARCEIDDDFCNPNPCENGASCFNMHRDYYCHCSAEYHGKNCSQPRPKCTSPPCEGKFIFCFTRNCGKQRLSGSNVCGDHGKCISEPLGGFTCVCDPGYTGRYCHENINDCVSNPCKHGGTCVDKINSFLCVCKEGWEGPFCNTNKNDCEPNPCHNNGTCIDSIADFICECRHGWKGKTCNFRHSHCAVNPCANGGTCTDLGDNFVCQCPRGFEGHTCQIANNPSCLSSPCQNGGTCISVSMGSGFSCVCKEGYEGILCQNNIDDCNPNPCYNNGRCVDGINWFRCECAKGFAGPDCRINIDDCASSPCAEGSTCRDGIADFQCICPPGQTGRFCNIGEKIHCPQGQECVPNFHSTCFIPPCPSIGICVSKENSTKSSMAGLIPNTKCRPNSAELGNNCATVTLVFNRSKILQGLTVESLCTQIRRLPKQLNHTPVHTVFLLCDQVEGVPDSIEVAVSSNGDPSFETYKYVSNIAKWLADRIGRKRSNSTSLSAVMEVKVKTSLLASTHGPGYLVPVLVSLLALLLLACFIGVGLWYYRNHYRRKGSLVHHRQPLRHSISSKLEQIEEEEKSNNQNEENLRRYHNPLHTSNDLKEKLSSPNLSDSTEMTQVDNINDSSHIPQKIYKPQSVESQKNITPINITEKALEKDNNLKSHQRRPSGKEVIV

Target ORF information:

RefSeq Version	XM_013929059.1
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Definition	Limulus polyphemus protein jagged-1-like (LOC106468631), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013929059.1

ORF Insert Sequence:

ATGCGCAGGG CGGGTCCAGC GCCTCACGAA ACGTTTGGCT CCTCGTATCA GTGTACCTCT 
GTTCTTCAAG TTACTCTTTC CATCCTTCAC GTGCTGGACG CGTCCGGGTC GTTTGAATTA 
CAGATTTTAG GAATACAGAA CTCTAGGGGT GAACTACTGA ACAGAGCTTG CTGTGGGGGT 
GAGGCGAGAA AAGAAGAGGA GATCTGTCCT AGGGAATGTA ACACTTACTT TCGCCTGTGT 
CTGAAACAGT ATCAATCACA GATTACAGCC AAGGGATCGT GTACATTCGG CAGCACGTCT 
TCACCGGTTG CCGTCGGAAA TTCCTTTTCA GTTCACAACG AGCCGGAAAA AGAAGTGGTA 
CTCAAGCTGC CCTTTGAATT TAGATGGACG AAATCATTCA CGTTAATCCT TGAAGCAGTA 
GACCACAACA ACCGTTCAAA ATCATCTCGT GACGAAGTGA TCGAAAGACA TATGTATTCG 
GGCATTATTA CACCCAGCGC AGAGTGGCAC CAACTATCAC ACCCAGGTAC TACAGCTACT 
ATAAACTATA GAATACGTGT GACGTGCGAC CCTTACTATT ACAATTACAC GTGCACCAAA 
TTTTGTCGAC CGAGGCACGA CCGTTTTGGA CACTACACTT GTAGTAAACA AGGAGACAAG 
ATCTGTTTGG TGGGATGGAA AGGACCTAAC TGTGAAACAG CGGTTTGTAA GGAAGGGTGT 
CACCCGCAAC ATGGAAACTG TGAGAAACCT GGAGAATGCA AAAGGAACCT TCATCGTCCA 
TCACTCCAGC AGCTTGGAAC TTCTCTTACT CCACTGGAAG AAACGGGTAG AAAAAAACAC 
AGTGGAGAGT CCGGAAGACA CACCAGAGAC AGTTCGTCAT CGAATTTAGT GACCTTGGGC 
AGCGGTCAAC TACGTCAATC GAAAGGGTTT GACCTTCTTA TTATTTCACT GGAACCATCA 
GATGCTGACG AATGCGTAAA AGATCCTTGT ATCAACGCTC TCTTGTGTAA GAATGTGGTT 
GGAGATTATA TCTGTGAATG CCAACCTGGA TGGATGGGAA AAAATTGCGA TCACAACATT 
AATGATTGTG TTGGTCAATG TCAAAACGGA GCCACGTGTA TTGATTTGGT AAGCGACTAC 
CACTGCGCCT GCTTCCCAGG ATTTACAGGA AGGAACTGCC AGACCAACAT TGACGACTGT 
GCCAGCAACC CCTGTTTGAA CGGAGGAGAA TGTGTAGACC TTGTGGCTGC TTTCTCTTGC 
ATCTGCCCTG TTGGGTATAA AGGAGCGAGG TGCGAGATCG ATGACGATTT TTGTAATCCC 
AATCCCTGTG AAAATGGAGC GTCCTGTTTC AACATGCATA GGGATTACTA TTGCCATTGT 
TCTGCTGAAT ATCACGGAAA AAACTGCTCC CAACCAAGAC CCAAGTGTAC TTCTCCTCCT 
TGTGAAGGTA AGTTTATTTT CTGTTTTACA AGGAACTGTG GAAAACAGCG ACTTAGCGGT 
TCAAATGTCT GTGGAGATCA TGGCAAGTGT ATCAGTGAAC CACTAGGGGG ATTCACATGC 
GTCTGTGATC CAGGCTATAC TGGCAGATAT TGTCATGAAA ATATAAACGA CTGTGTTTCC 
AACCCTTGCA AACATGGTGG TACCTGTGTG GACAAAATAA ACTCATTCCT ATGTGTTTGT 
AAAGAAGGCT GGGAAGGACC TTTTTGTAAT ACGAATAAGA ACGATTGTGA ACCGAATCCC 
TGTCATAACA ATGGAACATG CATCGACTCA ATCGCGGACT TTATTTGTGA ATGTAGACAT 
GGGTGGAAGG GGAAAACTTG CAACTTTAGA CACAGTCATT GTGCTGTCAA TCCCTGCGCC 
AATGGAGGAA CATGTACAGA TCTAGGAGAC AACTTTGTAT GTCAATGCCC ACGTGGGTTT 
GAAGGCCACA CATGTCAGAT AGCTAATAAT CCCAGTTGTC TCTCCAGTCC ATGCCAAAAT 
GGTGGCACCT GTATAAGCGT AAGCATGGGA TCAGGGTTTT CCTGTGTATG CAAAGAAGGT 
TATGAAGGCA TATTATGTCA AAATAATATT GATGATTGTA ACCCCAACCC TTGCTACAAT 
AATGGAAGAT GTGTGGATGG CATAAACTGG TTTCGATGTG AATGTGCCAA AGGTTTTGCT 
GGACCAGATT GTCGTATTAA CATTGATGAC TGTGCTTCCT CTCCTTGTGC GGAGGGAAGC 
ACCTGCAGAG ATGGTATTGC TGACTTTCAA TGTATCTGTC CACCTGGTCA GACAGGAAGA 
TTTTGTAACA TAGGGGAAAA GATTCATTGT CCTCAGGGAC AGGAGTGTGT CCCTAATTTC 
CACAGTACCT GTTTTATTCC TCCTTGCCCT TCAATAGGAA TTTGTGTGTC CAAGGAAAAT 
TCCACCAAAA GCTCCATGGC TGGATTGATT CCAAACACAA AATGTAGACC AAATTCAGCA 
GAGTTGGGAA ACAACTGTGC TACTGTTACT TTAGTGTTCA ACCGGAGTAA AATACTACAG 
GGACTGACAG TTGAAAGTTT GTGTACACAG ATACGAAGGT TACCCAAGCA GCTGAACCAT 
ACACCAGTCC ATACAGTTTT TCTTCTTTGT GATCAAGTAG AAGGTGTGCC AGATAGCATA 
GAAGTGGCAG TGTCTAGTAA TGGAGATCCT TCCTTTGAAA CCTACAAATA CGTTAGTAAC 
ATTGCAAAAT GGCTGGCAGA CAGAATAGGC CGTAAACGAT CTAACAGTAC ATCCTTAAGT 
GCCGTGATGG AGGTGAAAGT AAAGACCTCT CTTCTTGCTT CCACTCATGG ACCTGGCTAC 
TTGGTCCCAG TGCTGGTGTC CCTCTTAGCT TTACTGCTGC TAGCTTGTTT CATTGGGGTC 
GGACTCTGGT ATTATCGTAA TCATTATCGA AGAAAAGGAT CCTTGGTCCA CCATCGTCAG 
CCGTTACGAC ACTCAATTAG CAGTAAATTA GAGCAGATTG AAGAAGAAGA AAAGTCTAAT 
AACCAGAATG AAGAAAATCT GCGACGATAC CACAATCCTC TTCACACAAG TAATGATTTG 
AAGGAAAAGC TGAGCTCGCC AAATCTGAGC GATTCCACAG AGATGACACA AGTAGACAAC 
ATTAATGACT CCTCCCACAT TCCTCAAAAA ATATACAAAC CCCAATCTGT AGAATCACAA 
AAAAACATCA CTCCCATAAA TATTACAGAA AAAGCCTTGG AGAAAGACAA CAATCTTAAA 
TCTCATCAAA GAAGGCCATC TGGAAAAGAA GTCATAGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106468631 ( XM_022396976.1 ) cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab) -> XP_022252684.1 Limulus polyphemus protein jagged-1-like (LOC106468631), mRNA.

Price & Availability↑

CloneID	OLi99962
Clone ID Related Accession (Same CDS sequence)	XM_022396976.1
Accession Version	XM_022396976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504022400000
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Product	protein jagged-1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013668699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Limulus polyphemus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGCTAGGAT ATCACTATCT TTGTGCTAAA ATGTTTGCGA TCTTTTTAGT TACTCTTTCC 
ATCCTTCACG TGCTGGACGC GTCCGGGTCG TTTGAATTAC AGATTTTAGG AATACAGAAC 
TCTAGGGGTG AACTACTGAA CAGAGCTTGC TGTGGGGGTG AGGCGAGAAA AGAAGAGGAG 
ATCTGTCCTA GGGAATGTAA CACTTACTTT CGCCTGTGTC TGAAACAGTA TCAATCACAG 
ATTACAGCCA AGGGATCGTG TACATTCGGC AGCACGTCTT CACCGGTTGC CGTCGGAAAT 
TCCTTTTCAG TTCACAACGA GCCGGAAAAA GAAGTGGTAC TCAAGCTGCC CTTTGAATTT 
AGATGGACGA AATCATTCAC GTTAATCCTT GAAGCAGTAG ACCACAACAA CCGTTCAAAA 
TCATCTCGTG ACGAAGTGAT CGAAAGACAT ATGTATTCGG GCATTATTAC ACCCAGCGCA 
GAGTGGCACC AACTATCACA CCCAGGTACT ACAGCTACTA TAAACTATAG AATACGTGTG 
ACGTGCGACC CTTACTATTA CAATTACACG TGCACCAAAT TTTGTCGACC GAGGCACGAC 
CGTTTTGGAC ACTACACTTG TAGTAAACAA GGAGACAAGA TCTGTTTGGT GGGATGGAAA 
GGACCTAACT GTGAAACAGC GGTTTGTAAG GAAGGGTGTC ACCCGCAACA TGGAAACTGT 
GAGAAACCTG GAGAATGCAA AAGGAACCTT CATCGTCCAT CACTCCAGCA GCTTGGAACT 
TCTCTTACTC CACTGGAAGA AACGGGTAGA AAAAAACACA GTGGAGAGTC CGGAAGACAC 
ACCAGAGACA GTGCAATGGT TATTTCACTG GAACCATCAG ATGCTGACGA ATGCGTAAAA 
GATCCTTGTA TCAACGCTCT CTTGTGTAAG AATGTGGTTG GAGATTATAT CTGTGAATGC 
CAACCTGGAT GGATGGGAAA AAATTGCGAT CACAACATTA ATGATTGTGT TGGTCAATGT 
CAAAACGGAG CCACGTGTAT TGATTTGGTA AGCGACTACC ACTGCGCCTG CTTCCCAGGA 
TTTACAGGAA GGAACTGCCA GACCAACATT GACGACTGTG CCAGCAACCC CTGTTTGAAC 
GGAGGAGAAT GTGTAGACCT TGTGGCTGCT TTCTCTTGCA TCTGCCCTGT TGGGTATAAA 
GGAGCGAGGT GCGAGATCGA TGACGATTTT TGTAATCCCA ATCCCTGTGA AAATGGAGCG 
TCCTGTTTCA ACATGCATAG GGATTACTAT TGCCATTGTT CTGCTGAATA TCACGGAAAA 
AACTGCTCCC AACCAAGACC CAAGTGTACT TCTCCTCCTT GTGAAGCTTA TGACGGTTGT 
ATGGTGCCTA TTTCTACTAA CACTTCCAGT GGAAAACAGC GACTTAGCGG TTCAAATGTC 
TGTGGAGATC ATGGCAAGTG TATCAGTGAA CCACTAGGGG GATTCACATG CGTCTGTGAT 
CCAGGCTATA CTGGCAGATA TTGTCATGAA AATATAAACG ACTGTGTTTC CAACCCTTGC 
AAACATGGTG GTACCTGTGT GGACAAAATA AACTCATTCC TATGTGTTTG TAAAGAAGGC 
TGGGAAGGAC CTTTTTGTAA TACGAATAAG AACGATTGTG AACCGAATCC CTGTCATAAC 
AATGGAACAT GCATCGACTC AATCGCGGAC TTTATTTGTG AATGTAGACA TGGGTGGAAG 
GGGAAAACTT GCAACTTTAG ACACAGTCAT TGTGCTGTCA ATCCCTGCGC CAATGGAGGA 
ACATGTACAG ATCTAGGAGA CAACTTTGTA TGTCAATGCC CACGTGGGTT TGAAGGCCAC 
ACATGTCAGA TAGCTAATAA TCCCAGTTGT CTCTCCAGTC CATGCCAAAA TGGTGGCACC 
TGTATAAGCG TAAGCATGGG ATCAGGGTTT TCCTGTGTAT GCAAAGAAGG TTATGAAGGC 
ATATTATGTC AAAATAATAT TGATGATTGT AACCCCAACC CTTGCTACAA TAATGGAAGA 
TGTGTGGATG GCATAAACTG GTTTCGATGT GAATGTGCCA AAGGTTTTGC TGGACCAGAT 
TGTCGTATTA ACATTGATGA CTGTGCTTCC TCTCCTTGTG CGGAGGGAAG CACCTGCAGA 
GATGGTATTG CTGACTTTCA ATGTATCTGT CCACCTGGTC AGACAGGAAG ATTTTGTAAC 
ATAGAGGTAG GGAAGAGACA GTGTGAATGG AATGGATTTA TATACAATCA TGGCGCTACA 
TGGGATGAAG ATTGTAACAC ATGTCTCTGT CATGACACTG AAGTCACCTG CACCAAGTTT 
TGGTGTGGCT TTCAAAATTG CTTGGCTGAC CCAAACTCAA CAGGGGAAAA GATTCATTGT 
CCTCAGGGAC AGGAGTGTGT CCCTAATTTC CACAGTACCT GTTTTATTCC TCCTTGCCCT 
TCAATAGGAA TTTGTGTGTC CAAGGAAAAT TCCACCAAAA GCTCCATGGC TGGATTGATT 
CCAAACACAA AATGTAGACC AAATTCAGCA GAGTTGGGAA ACAACTGTGC TACTGTTACT 
TTAGTGTTCA ACCGGAGTAA AATACTACAG GGACTGACAG TTGAAAGTTT GTGTACACAG 
ATACGAAGGT TACCCAAGCA GCTGAACCAT ACACCAGTCC ATACAGTTTT TCTTCTTTGT 
GATCAAGTAG AAGGTGTGCC AGATAGCATA GAAGTGGCAG TGTCTAGTAA TGGAGATCCT 
TCCTTTGAAA CCTACAAATA CGTTAGTAAC ATTGCAAAAT GGCTGGCAGA CAGAATAGGC 
CGTAAACGAT CTAACAGTAC ATCCTTAAGT GCCGTGATGG AGGTGAAAGT AAAGACCTCT 
CTTCTTGCTT CCACTCATGG ACCTGGCTAC TTGGTCCCAG TGCTGGTGTC CCTCTTAGCT 
TTACTGCTGC TAGCTTGTTT CATTGGGGTC GGACTCTGGT ATTATCGTAA TCATTATCGA 
AGAAAAGGAT CCTTGGTCCA CCATCGTCAG CCGTTACGAC ACTCAATTAG CAGTAAATTA 
GAGCAGATTG AAGAAGAAGA AAAGTCTAAT AACCAGAATG AAGAAAATCT GCGACGATAC 
CACAATCCTC TTCACACAAG TAATGATTTG AAGGAAAAGC TGAGCTCGCC AAATCTGAGC 
GATTCCACAG AGATGACACA AGTAGACAAC ATTAATGACT CCTCCCACAT TCCTCAAAAA 
ATATACAAAC CCCAATCTGT AGAATCACAA AAAAACATCA CTCCCATAAA TATTACAGAA 
AAAGCCTTGG AGAAAGACAA CAATCTTAAA TCTCATCAAA GAAGGCCATC TGGAAAAGAA 
GTCATAGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022252684.1
CDS	360..3731
Translation	MLGYHYLCAKMFAIFLVTLSILHVLDASGSFELQILGIQNSRGELLNRACCGGEARKEEEICPRECNTYFRLCLKQYQSQITAKGSCTFGSTSSPVAVGNSFSVHNEPEKEVVLKLPFEFRWTKSFTLILEAVDHNNRSKSSRDEVIERHMYSGIITPSAEWHQLSHPGTTATINYRIRVTCDPYYYNYTCTKFCRPRHDRFGHYTCSKQGDKICLVGWKGPNCETAVCKEGCHPQHGNCEKPGECKRNLHRPSLQQLGTSLTPLEETGRKKHSGESGRHTRDSAMVISLEPSDADECVKDPCINALLCKNVVGDYICECQPGWMGKNCDHNINDCVGQCQNGATCIDLVSDYHCACFPGFTGRNCQTNIDDCASNPCLNGGECVDLVAAFSCICPVGYKGARCEIDDDFCNPNPCENGASCFNMHRDYYCHCSAEYHGKNCSQPRPKCTSPPCEAYDGCMVPISTNTSSGKQRLSGSNVCGDHGKCISEPLGGFTCVCDPGYTGRYCHENINDCVSNPCKHGGTCVDKINSFLCVCKEGWEGPFCNTNKNDCEPNPCHNNGTCIDSIADFICECRHGWKGKTCNFRHSHCAVNPCANGGTCTDLGDNFVCQCPRGFEGHTCQIANNPSCLSSPCQNGGTCISVSMGSGFSCVCKEGYEGILCQNNIDDCNPNPCYNNGRCVDGINWFRCECAKGFAGPDCRINIDDCASSPCAEGSTCRDGIADFQCICPPGQTGRFCNIEVGKRQCEWNGFIYNHGATWDEDCNTCLCHDTEVTCTKFWCGFQNCLADPNSTGEKIHCPQGQECVPNFHSTCFIPPCPSIGICVSKENSTKSSMAGLIPNTKCRPNSAELGNNCATVTLVFNRSKILQGLTVESLCTQIRRLPKQLNHTPVHTVFLLCDQVEGVPDSIEVAVSSNGDPSFETYKYVSNIAKWLADRIGRKRSNSTSLSAVMEVKVKTSLLASTHGPGYLVPVLVSLLALLLLACFIGVGLWYYRNHYRRKGSLVHHRQPLRHSISSKLEQIEEEEKSNNQNEENLRRYHNPLHTSNDLKEKLSSPNLSDSTEMTQVDNINDSSHIPQKIYKPQSVESQKNITPINITEKALEKDNNLKSHQRRPSGKEVIV

Target ORF information:

RefSeq Version	XM_022396976.1
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Definition	Limulus polyphemus protein jagged-1-like (LOC106468631), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022396976.1

ORF Insert Sequence:

ATGCTAGGAT ATCACTATCT TTGTGCTAAA ATGTTTGCGA TCTTTTTAGT TACTCTTTCC 
ATCCTTCACG TGCTGGACGC GTCCGGGTCG TTTGAATTAC AGATTTTAGG AATACAGAAC 
TCTAGGGGTG AACTACTGAA CAGAGCTTGC TGTGGGGGTG AGGCGAGAAA AGAAGAGGAG 
ATCTGTCCTA GGGAATGTAA CACTTACTTT CGCCTGTGTC TGAAACAGTA TCAATCACAG 
ATTACAGCCA AGGGATCGTG TACATTCGGC AGCACGTCTT CACCGGTTGC CGTCGGAAAT 
TCCTTTTCAG TTCACAACGA GCCGGAAAAA GAAGTGGTAC TCAAGCTGCC CTTTGAATTT 
AGATGGACGA AATCATTCAC GTTAATCCTT GAAGCAGTAG ACCACAACAA CCGTTCAAAA 
TCATCTCGTG ACGAAGTGAT CGAAAGACAT ATGTATTCGG GCATTATTAC ACCCAGCGCA 
GAGTGGCACC AACTATCACA CCCAGGTACT ACAGCTACTA TAAACTATAG AATACGTGTG 
ACGTGCGACC CTTACTATTA CAATTACACG TGCACCAAAT TTTGTCGACC GAGGCACGAC 
CGTTTTGGAC ACTACACTTG TAGTAAACAA GGAGACAAGA TCTGTTTGGT GGGATGGAAA 
GGACCTAACT GTGAAACAGC GGTTTGTAAG GAAGGGTGTC ACCCGCAACA TGGAAACTGT 
GAGAAACCTG GAGAATGCAA AAGGAACCTT CATCGTCCAT CACTCCAGCA GCTTGGAACT 
TCTCTTACTC CACTGGAAGA AACGGGTAGA AAAAAACACA GTGGAGAGTC CGGAAGACAC 
ACCAGAGACA GTGCAATGGT TATTTCACTG GAACCATCAG ATGCTGACGA ATGCGTAAAA 
GATCCTTGTA TCAACGCTCT CTTGTGTAAG AATGTGGTTG GAGATTATAT CTGTGAATGC 
CAACCTGGAT GGATGGGAAA AAATTGCGAT CACAACATTA ATGATTGTGT TGGTCAATGT 
CAAAACGGAG CCACGTGTAT TGATTTGGTA AGCGACTACC ACTGCGCCTG CTTCCCAGGA 
TTTACAGGAA GGAACTGCCA GACCAACATT GACGACTGTG CCAGCAACCC CTGTTTGAAC 
GGAGGAGAAT GTGTAGACCT TGTGGCTGCT TTCTCTTGCA TCTGCCCTGT TGGGTATAAA 
GGAGCGAGGT GCGAGATCGA TGACGATTTT TGTAATCCCA ATCCCTGTGA AAATGGAGCG 
TCCTGTTTCA ACATGCATAG GGATTACTAT TGCCATTGTT CTGCTGAATA TCACGGAAAA 
AACTGCTCCC AACCAAGACC CAAGTGTACT TCTCCTCCTT GTGAAGCTTA TGACGGTTGT 
ATGGTGCCTA TTTCTACTAA CACTTCCAGT GGAAAACAGC GACTTAGCGG TTCAAATGTC 
TGTGGAGATC ATGGCAAGTG TATCAGTGAA CCACTAGGGG GATTCACATG CGTCTGTGAT 
CCAGGCTATA CTGGCAGATA TTGTCATGAA AATATAAACG ACTGTGTTTC CAACCCTTGC 
AAACATGGTG GTACCTGTGT GGACAAAATA AACTCATTCC TATGTGTTTG TAAAGAAGGC 
TGGGAAGGAC CTTTTTGTAA TACGAATAAG AACGATTGTG AACCGAATCC CTGTCATAAC 
AATGGAACAT GCATCGACTC AATCGCGGAC TTTATTTGTG AATGTAGACA TGGGTGGAAG 
GGGAAAACTT GCAACTTTAG ACACAGTCAT TGTGCTGTCA ATCCCTGCGC CAATGGAGGA 
ACATGTACAG ATCTAGGAGA CAACTTTGTA TGTCAATGCC CACGTGGGTT TGAAGGCCAC 
ACATGTCAGA TAGCTAATAA TCCCAGTTGT CTCTCCAGTC CATGCCAAAA TGGTGGCACC 
TGTATAAGCG TAAGCATGGG ATCAGGGTTT TCCTGTGTAT GCAAAGAAGG TTATGAAGGC 
ATATTATGTC AAAATAATAT TGATGATTGT AACCCCAACC CTTGCTACAA TAATGGAAGA 
TGTGTGGATG GCATAAACTG GTTTCGATGT GAATGTGCCA AAGGTTTTGC TGGACCAGAT 
TGTCGTATTA ACATTGATGA CTGTGCTTCC TCTCCTTGTG CGGAGGGAAG CACCTGCAGA 
GATGGTATTG CTGACTTTCA ATGTATCTGT CCACCTGGTC AGACAGGAAG ATTTTGTAAC 
ATAGAGGTAG GGAAGAGACA GTGTGAATGG AATGGATTTA TATACAATCA TGGCGCTACA 
TGGGATGAAG ATTGTAACAC ATGTCTCTGT CATGACACTG AAGTCACCTG CACCAAGTTT 
TGGTGTGGCT TTCAAAATTG CTTGGCTGAC CCAAACTCAA CAGGGGAAAA GATTCATTGT 
CCTCAGGGAC AGGAGTGTGT CCCTAATTTC CACAGTACCT GTTTTATTCC TCCTTGCCCT 
TCAATAGGAA TTTGTGTGTC CAAGGAAAAT TCCACCAAAA GCTCCATGGC TGGATTGATT 
CCAAACACAA AATGTAGACC AAATTCAGCA GAGTTGGGAA ACAACTGTGC TACTGTTACT 
TTAGTGTTCA ACCGGAGTAA AATACTACAG GGACTGACAG TTGAAAGTTT GTGTACACAG 
ATACGAAGGT TACCCAAGCA GCTGAACCAT ACACCAGTCC ATACAGTTTT TCTTCTTTGT 
GATCAAGTAG AAGGTGTGCC AGATAGCATA GAAGTGGCAG TGTCTAGTAA TGGAGATCCT 
TCCTTTGAAA CCTACAAATA CGTTAGTAAC ATTGCAAAAT GGCTGGCAGA CAGAATAGGC 
CGTAAACGAT CTAACAGTAC ATCCTTAAGT GCCGTGATGG AGGTGAAAGT AAAGACCTCT 
CTTCTTGCTT CCACTCATGG ACCTGGCTAC TTGGTCCCAG TGCTGGTGTC CCTCTTAGCT 
TTACTGCTGC TAGCTTGTTT CATTGGGGTC GGACTCTGGT ATTATCGTAA TCATTATCGA 
AGAAAAGGAT CCTTGGTCCA CCATCGTCAG CCGTTACGAC ACTCAATTAG CAGTAAATTA 
GAGCAGATTG AAGAAGAAGA AAAGTCTAAT AACCAGAATG AAGAAAATCT GCGACGATAC 
CACAATCCTC TTCACACAAG TAATGATTTG AAGGAAAAGC TGAGCTCGCC AAATCTGAGC 
GATTCCACAG AGATGACACA AGTAGACAAC ATTAATGACT CCTCCCACAT TCCTCAAAAA 
ATATACAAAC CCCAATCTGT AGAATCACAA AAAAACATCA CTCCCATAAA TATTACAGAA 
AAAGCCTTGG AGAAAGACAA CAATCTTAAA TCTCATCAAA GAAGGCCATC TGGAAAAGAA 
GTCATAGTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC106468631 cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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