LOC106466475 cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106466475
Entrez Gene ID	106466475
Full Name	HEAT repeat-containing protein 5B-like
Gene Type	protein-coding
Organism	Limulus polyphemus(Atlantic horseshoe crab)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013926768.1	XP_013782222.1	HEAT repeat-containing protein 5B-like
XM_022394496.1	XP_022250204.1	HEAT repeat-containing protein 5B-like isoform X4
XM_022394494.1	XP_022250202.1	HEAT repeat-containing protein 5B-like isoform X2
XM_022394495.1	XP_022250203.1	HEAT repeat-containing protein 5B-like isoform X3
XM_022394493.1	XP_022250201.1	HEAT repeat-containing protein 5B-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC106466475 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106466475 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLi10917	XM_013926768.1 Latest version!	Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OLi98325	XM_022394493.1 Latest version!	Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OLi98326	XM_022394494.1 Latest version!	Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OLi98327	XM_022394495.1 Latest version!	Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OLi98328	XM_022394496.1 Latest version!	Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106466475 ( XM_013926768.1 ) cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab) -> XP_013782222.1 Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), mRNA.

Price & Availability↑

CloneID	OLi10917
Clone ID Related Accession (Same CDS sequence)	XM_013926768.1
Accession Version	XM_013926768.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1441641600000
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Product	HEAT repeat-containing protein 5B-like
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013667881.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Limulus polyphemus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAC TATCCCACAG TTTGACTCTA AATGAGGAGG CATTGAGTCA AATAGCTGAA 
GCAAAAAGGC CAGTCTTTGT TTTTGAATGG TTACGATTTT TAGATAAAGT TCTTGGAGCA 
GCTCAGAAGT CAGACATTAA GGAATGCCAG AAAAAACTAG TAGAACAGTT GTTGAATCAA 
GTCCACGAGT CACCAGGACC TCCTACCAGA AGACTAATCG CAAGATGTCT TGCAACACTC 
TTCAGTGTTG GAGATACATT TCTCCTCTTC GAAGCAGTCA ATAGATGTAC AGATATTTTG 
AAAAACAAAG ATGATTCCCC CAGCGTCTTA CCGACAAGAC TGGGGGCAAT TACCTGTATA 
GGAGCCATGT ATGAAAAGCT GGGAAGAATG ATGGGGAGAT CACACGAAGA AGCTGTCACA 
GTTATGCTGA AGTCTCTTAA GAATGCCGAG TCACAGACTC GCTGTGAAAC AATGGTAACT 
ATGGAGAAGA TTATTGCTGG TATGGGAACA GCTGCTGCAT CTGTTCATAA AGATATCTTT 
AAAGCTGCTA AGCACTGTAT GACTGACCGT GTTATGCCAG TAAGATGCGC AGCTGCCCAG 
TGTTTACAGG AGATGCTGAA ACATGTACCA TTTGTTTATA CATCTGAACT GGAGACTGTG 
GCTTCCCTGT GTTTTCGATC CTTTGAAGGG TCTAACTACG AAGTTCGATG TGCTGTTGCT 
AATCTGCTTG GAACACTTGT TGCTACCACT CAACAGGCTT CACAGCCTGC AGTGGGTAAA 
AATCGCCTCG CAACATTCGA AGAAGTGTTA GCTTTGATGT CCTCGGGATT TCTTCGTGGT 
GGTATTGGTT TCTTGAAAGG TGGTGCAGGA GAGATGATTA AAGGTAGTTC TAATGTCAGT 
AGAGAAATCA GGGTTGGAGT GACACATGCT TATGTTGTTT TGGTTCGACA ACTTGGAGGC 
TTGTGGTTGG AAAGGAACCT GAATACCCTC TTAGTCCACC TTCTGGAGCT TGTTTCTAGT 
CCTAGAGCAG CAACCTCACA TGTTGATGCC GTTTACAGCC GCAAATGCAT TGCCTTCATC 
TTGCGTTCAG TTTTGGGCAA GATGCTAGGA GAAAAGGCTC AGGCAGCAGC TTGCAAAGAC 
TTAGTACAGA TTATTGCGAA GTATTCAAAT GCAGATGCTG CAGCAGAGTC CTCTGGCAGT 
AAAGAAGGCT TACAAGAAGC AGCCCTTGCT CAACATGTTC TCGTGTGTGC TCTGCAAGAG 
CTTGGTTGTT TAATATCTAG TCTTGGAACC ACAGCCTCTT CGGTAGTGAA TGATTCTTCC 
TCAGGTATAA TTGAAGCTGT GGTATCAGTT TTAATTCACT CAAGTGCAGC ACGACTTGCA 
GCTGCGTGGT GCTTGAGGTC CATTGCAACA GCTGTATCAT CTCAGCTAAC TCCATTGCTA 
GACCGATGTC TAGAGCAACT AGATACCCTT AAAGCGTCAC CCGAGGCTAT TTGTGGATAT 
TCTGGAGCTT TGTCGGCACT TCTTGGAGCA GCATGTTATA CTGCATTAGG CATCCCTCAC 
AGTAAAGGAA AGATCATATT CAATGTAGCG GAGGATCTTC TTCGCACAGC ATCACAGAAT 
AGTAGACTGT CTGTCCAACG TACTCAAGCT GGTTGGTTAA TGATTGGAGC AGTCATGACA 
TTGGGTCCAC CTGTGGTTCG TGGACTTCTG CCAAGAATGT TGCTTCTTTG GAAGAACTCT 
TTTCCAAGGT CAACAAAAGA ACTAGAATCA GAAAAGGCTA GAGGAGATGC TTTCACATGG 
CAAGTAACCC TTGAAGGAAG AGCAGGAGCA TTAGCAGCTA TGGCAAGTTT TCTGGTACAC 
TGTAAAGAAC TTGTCTCTGA AGAGGTGGTG CGTAGATTGC TGTCACCAGT TGAAGCTGCT 
CTTGCCATGC TAACAAGCTT AACAAACCTG GTCAAGTCAT ATGGACAGCA CTTGAAGGCA 
GCTGTTGCGA TGGTGAGACT ACGTCTTTAT GAAGTGCTAA CACTTTTGCC TGCCCAATCA 
TATGAAGGAA GTTACACCAG TCTTTTAAGG CTTTTGGTGG CTGAGTTTAC CCTGACTGAA 
AACCCAGCTA ATACTACTAC ATCGTTGCTT CGAAGCCTTT GCCACACTGA TGATAGTGTG 
ATATTGGGGT CCTGGCTGCA AGAAACTGAT CACAAGGCTA TTGAGGACCA GCTTCAACCA 
AATAGTGCAT CGGGATCAGG TGCATTGGAA CACGATTTGA CAGCTCTGTA TAAGTCACTG 
CCAAAGGGGG AGTCAATTCC AGGTCCTTTA CCATTAGGAG TTGCTGTTAT TGACATGTCT 
GTCATCCTGT TTGGCCAGAC ATTTCCTTAT GTAGCTGTTA AGCACAGACT GCAGATGTTA 
GGTCACTTCT CACAATGTAT TAGGCACACT AAGGCCTCAA GACAGGAAGC TGTTCAAATT 
AACATTTTCA CAGCTGTTCT AAGTGCTCTA AAGATGCTTG CAGAGACAAA AACTCCACTA 
GGACCTGATG ACGTGAGGCA AGCTGCTGCT AGTTTAGTGC TGGGAGCCTT GAGTCACCCA 
AATCCTATAC TACGCTGTGC CGCAGGAGAG GCTTTAGGAC GAATGGCACA GGTTGTGGGT 
GATGGCCGGT TTGTTGCAGA GATGGCTCAG CACAGCTTTG ACAAATTAAA AACGGCCAGA 
GATGCTGTAT CACGTACAGG CCACTCTCTA ACACTAGGAT GTTTACACCG ATACGTTGGG 
GGAATGGGAT CAGGCCAACA TCTTAACACT AGTGTTAGTA TTTTGTTGGC ATTAGCCAAT 
GATACCAATG CTCCTGTTGT TCAGGTATGG GCTCTTCATG CTATAGCCCT CATAGCAGAT 
TCTGGGGGAC CAATGTTCCG TGGATATGTG GAGCCTACCC TCTCTCTAGC TTTAAAATTA 
CTACTGAATA TCCCTCCTTC CAATGTAGAC GTATTGCAGT GTATAGGAAA GAGTCTGTCT 
GCTTTGATTA CTACTGTGGG ACCAGAATTA CAAGGAAATA CGAACTCCAT CAGCACTGCC 
CGTTCTTCCT TTTTGGTAGC CTGTGCCATA ATGCAAGATC ATGGTGACTC CCTAGTCCAT 
GCTGAAGCCA TTTCTTGCCT TCAGCAGTTA CACATGTTTG CTCCACGTCA CGTTAATCTA 
TCAACCCTTG TACCTACCCT TTGTGTGAGT ACATGTGTAT TATACTCTTC CCATCTTCTG 
TTAAGAAGAG CAGCAGTAGC CTGCTTAAGA CAGCTGTCCC AGAGAGAGGC TAAGGAAGTG 
TGTGAACATG CTCTTTCCTG GGCTAGAGAA TCGGGTGGCA CCACAACTGG CTACAACTCT 
AATATCAGCA TTCCTGCAAT AGGAGAGACT GGGCTACCTG GTGTACTCTT TGCCATGCTG 
GACGTAGAGA ATGACCTGAG GCTAGTAGGT CACATCAAGG ACACACTTAC CAGCATGTTA 
CAGGCACTAG CGAGGGAGAA CCTAGTTCAG TGGTTGAGTT TATGTAAAGA TGTTCTTACT 
GCTGCTGATT CTAGTTCATC CAGCATATCA TCTTTGAAAG AAGCTGAGAG AGAATTAGGA 
GATAATGAAG CAGATGTAGA TGACGAGGAG AGATTTAAAG CTTCAGAAGA GCCAGTATCG 
CATCCTTTTG TCTCTCCTCG TTGGCCCACT AGAGTGTTTT CTGCCATTTC TTTGAGGAGG 
ATTATTGAAG CATGTGATGG CAATCGAGCT CATTTTGATT TAGCCATGGC AAGGGAACAG 
AAATTGATTA GACCAAAAGA AGATTTTTTG GTTCTTCATC TGTCTGACCT GATTAGGATG 
GCATTTATGG CAGCTACATC AGAAAGTGAC CCTTTAAGGT TGGAAGGATT AAGAGCATTG 
CAGTTAATTA TTGACAAGTT TTCCAAAGTT CCTGAACCAG AATTTCCAGG TCATGTAATC 
TTGGAACAGT ATCAAGCTCA GGTTGGGGCT GCTCTTCGAC CAGCATTCTC ACCAGAAACA 
CCTTCTCATG TTACTGCTAT GGCTTGTCAG GTGTGTAGTG CCTGGATTGG AAGTGGTGTG 
GCACGAGACT TGAACGATCT TCGACGCGTT CACCAGCTCT TGGTGTCATC TCTGAACAAG 
CTTCATAAAG GGACCAGCTC TCACCTGTAC AATGAGAGTG CTTCTACTCT AGAGAAGCTG 
GCAATATTGA AAGCATGGTC TGAGGTTTTT GTTGTTGCAA TGGAACAGGA AAAACAACTA 
AGCAATGTGC CACCATCCCA AGAGGAACCT CAAAACGATG ATGATGGCAG TTGTGGACAA 
GAAAGTCTCT TAAAGCTTGT AACACCTGAA CTTGTATCTT TAAGCAAGCA CTGGCTTGCT 
GCTTTAAAGG ATCATGCATT ACTCTCACTT CCTGCAGAGT TTTCCAGTCA GTTACCCCAT 
GACGGAGGAG CATTTTACAC CCCTGATACT ATGGATTCTG CTCGACAGTA TTATCGAAAG 
GCCTGGCCAC CCATCCTTCA TGCTGCAGCT CTTTGGCTCT GCACAGGAGG GTTTGAAGGT 
CTTGAACGAG AGTTTGAGCA AGATGCAAAC AACAGCGGAA CAATTTTAGG ACCTCCTGGA 
AGCCCTATGA ACAAAGTTAT TTCAAATCCT AATTCTGAGA GATTTCATCT GCTCTTTGGT 
ATCTGTATGG AGGCACTGTG TAGTCCTCGG TCGGCAGAAA GCCTAGAATG TGTACTTGTC 
TGTTTACAAG CATTACAGCT GCTTTTGAAA CATCCATTGC CAAAAAGCAT TCTTGGCCAG 
GATCCTCAGT TGTGCATTGA ACTGTGTAAT GTTTTACATA GAATTTTGCT TACCCGGGAA 
ACTCCCCGAT GCCAGATGAT TGTGATGAAA ATTGCCCATC TTTTGCTTGA AGCAAGACAT 
GATGACATTC GTTCTCATAA AAAGAAGAAA CTGAAAGAGC TTGCCCCTGC TAATCAAGAA 
CTAGAAGATG TTGACACAGC ATTAGCTGCA CTTGGAGAAG GTGGTGAAAC TGGTGACATA 
GTACCGGGAC AGTCAGTAGT GTTTGCTCTA CTCGAGGTGT GTCTGTGTTT GTTAGTCAGG 
CAGCTCCCTC ATATCAGTCC TGCACTAGCT AACAATACAG GCACGTTTGG CATTCAGGCA 
GCCACACGAT CCAAAAAGTT GACAGAAGAA TCTTCAAAGT TGGTTGCTGC TACTCTGAGT 
GTCATGGCTG AACTTCCAAA CCTTTGTTCG CCCAAAGGTA GCACCACTGT CCTCCCGACC 
ATTCTCTACT TGACAACAGG AGTACTAAAA GAAACAGCTG TTCAGACTAC TGAAGACAGA 
GGATTAGTGG TAGCCTCGCC TCCAGTAAGC ACCACATTGC AAAGTCTGAA ATCTCTCTGC 
ACCTCACCTT TCACAAGGAA CAAACTGTGT GGTTCAGAGT GGCTCACTTT GCTCCAGAGT 
GCTTTAGCTC GGATTTTGGA TCTCAGTAAG ACAAGTTGTG AAGATAGTAA ACCAGATGAA 
ATTTCCATGT TGTTGGCTGT GGCAGTTTTC ATTCTCTCAG CTCCTGGAGA AGTTGTGTCA 
ACACCAAATC TTCAGTTTCC ATGTATCAAT CTTTTTAAAC AAGCACTGCA GCTCGATGGA 
GACCTGGTAC AGCTTAAATG TGTTCAGACA CTCCGTTCCA TGTTTCAACA CCCTGATCGA 
AGTATAGCCA CTCCATACAT CCATACTCTT GCTCCACGAG TCGTGGAAAT ACTTGTGCAG 
TTGACTGCTG ATTTGACAAC TCAGTCTACT GTTACTGATC GCCAGTTTAG TCTAGCCACA 
GAATGTCTGA CCTGTCTAGA CACACTGGTT ACTCTGGCTC CTTCTGATAA AAAAATTCAT 
ATGCTGAGTC TGTACATCCC CCTGGTGGTC AACCTTTTGA TAGAGCCAAC ATTACAACAG 
GGGGCGTCAC ACAACAGGAA GAAGTTACAC GACCATGCTT TGCAACAGCT GATCAAAGTG 
GGCCCTCAAT ATCCCCAGGA CTTCCGCTCC ATCATGACCC AAGCTCCCCA GCTGAGAGGG 
CGGCTTGAAA ATGCCATAAG GAAGGGTTCG CTAATTCACG TTGGAAGCCG AGAATCTTCC 
ACCGCACCTT CGAGTCAAAA AATAAATTCC TCCCAAGCTC CAGCAATCAA ATTAAAGATG 
GATTTTAGCA ATTTCACTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013782222.1
CDS	69..6272
Translation	MMELSHSLTLNEEALSQIAEAKRPVFVFEWLRFLDKVLGAAQKSDIKECQKKLVEQLLNQVHESPGPPTRRLIARCLATLFSVGDTFLLFEAVNRCTDILKNKDDSPSVLPTRLGAITCIGAMYEKLGRMMGRSHEEAVTVMLKSLKNAESQTRCETMVTMEKIIAGMGTAAASVHKDIFKAAKHCMTDRVMPVRCAAAQCLQEMLKHVPFVYTSELETVASLCFRSFEGSNYEVRCAVANLLGTLVATTQQASQPAVGKNRLATFEEVLALMSSGFLRGGIGFLKGGAGEMIKGSSNVSREIRVGVTHAYVVLVRQLGGLWLERNLNTLLVHLLELVSSPRAATSHVDAVYSRKCIAFILRSVLGKMLGEKAQAAACKDLVQIIAKYSNADAAAESSGSKEGLQEAALAQHVLVCALQELGCLISSLGTTASSVVNDSSSGIIEAVVSVLIHSSAARLAAAWCLRSIATAVSSQLTPLLDRCLEQLDTLKASPEAICGYSGALSALLGAACYTALGIPHSKGKIIFNVAEDLLRTASQNSRLSVQRTQAGWLMIGAVMTLGPPVVRGLLPRMLLLWKNSFPRSTKELESEKARGDAFTWQVTLEGRAGALAAMASFLVHCKELVSEEVVRRLLSPVEAALAMLTSLTNLVKSYGQHLKAAVAMVRLRLYEVLTLLPAQSYEGSYTSLLRLLVAEFTLTENPANTTTSLLRSLCHTDDSVILGSWLQETDHKAIEDQLQPNSASGSGALEHDLTALYKSLPKGESIPGPLPLGVAVIDMSVILFGQTFPYVAVKHRLQMLGHFSQCIRHTKASRQEAVQINIFTAVLSALKMLAETKTPLGPDDVRQAAASLVLGALSHPNPILRCAAGEALGRMAQVVGDGRFVAEMAQHSFDKLKTARDAVSRTGHSLTLGCLHRYVGGMGSGQHLNTSVSILLALANDTNAPVVQVWALHAIALIADSGGPMFRGYVEPTLSLALKLLLNIPPSNVDVLQCIGKSLSALITTVGPELQGNTNSISTARSSFLVACAIMQDHGDSLVHAEAISCLQQLHMFAPRHVNLSTLVPTLCVSTCVLYSSHLLLRRAAVACLRQLSQREAKEVCEHALSWARESGGTTTGYNSNISIPAIGETGLPGVLFAMLDVENDLRLVGHIKDTLTSMLQALARENLVQWLSLCKDVLTAADSSSSSISSLKEAERELGDNEADVDDEERFKASEEPVSHPFVSPRWPTRVFSAISLRRIIEACDGNRAHFDLAMAREQKLIRPKEDFLVLHLSDLIRMAFMAATSESDPLRLEGLRALQLIIDKFSKVPEPEFPGHVILEQYQAQVGAALRPAFSPETPSHVTAMACQVCSAWIGSGVARDLNDLRRVHQLLVSSLNKLHKGTSSHLYNESASTLEKLAILKAWSEVFVVAMEQEKQLSNVPPSQEEPQNDDDGSCGQESLLKLVTPELVSLSKHWLAALKDHALLSLPAEFSSQLPHDGGAFYTPDTMDSARQYYRKAWPPILHAAALWLCTGGFEGLEREFEQDANNSGTILGPPGSPMNKVISNPNSERFHLLFGICMEALCSPRSAESLECVLVCLQALQLLLKHPLPKSILGQDPQLCIELCNVLHRILLTRETPRCQMIVMKIAHLLLEARHDDIRSHKKKKLKELAPANQELEDVDTALAALGEGGETGDIVPGQSVVFALLEVCLCLLVRQLPHISPALANNTGTFGIQAATRSKKLTEESSKLVAATLSVMAELPNLCSPKGSTTVLPTILYLTTGVLKETAVQTTEDRGLVVASPPVSTTLQSLKSLCTSPFTRNKLCGSEWLTLLQSALARILDLSKTSCEDSKPDEISMLLAVAVFILSAPGEVVSTPNLQFPCINLFKQALQLDGDLVQLKCVQTLRSMFQHPDRSIATPYIHTLAPRVVEILVQLTADLTTQSTVTDRQFSLATECLTCLDTLVTLAPSDKKIHMLSLYIPLVVNLLIEPTLQQGASHNRKKLHDHALQQLIKVGPQYPQDFRSIMTQAPQLRGRLENAIRKGSLIHVGSRESSTAPSSQKINSSQAPAIKLKMDFSNFTG

Target ORF information:

RefSeq Version	XM_013926768.1
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Definition	Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013926768.1

ORF Insert Sequence:

ATGATGGAAC TATCCCACAG TTTGACTCTA AATGAGGAGG CATTGAGTCA AATAGCTGAA 
GCAAAAAGGC CAGTCTTTGT TTTTGAATGG TTACGATTTT TAGATAAAGT TCTTGGAGCA 
GCTCAGAAGT CAGACATTAA GGAATGCCAG AAAAAACTAG TAGAACAGTT GTTGAATCAA 
GTCCACGAGT CACCAGGACC TCCTACCAGA AGACTAATCG CAAGATGTCT TGCAACACTC 
TTCAGTGTTG GAGATACATT TCTCCTCTTC GAAGCAGTCA ATAGATGTAC AGATATTTTG 
AAAAACAAAG ATGATTCCCC CAGCGTCTTA CCGACAAGAC TGGGGGCAAT TACCTGTATA 
GGAGCCATGT ATGAAAAGCT GGGAAGAATG ATGGGGAGAT CACACGAAGA AGCTGTCACA 
GTTATGCTGA AGTCTCTTAA GAATGCCGAG TCACAGACTC GCTGTGAAAC AATGGTAACT 
ATGGAGAAGA TTATTGCTGG TATGGGAACA GCTGCTGCAT CTGTTCATAA AGATATCTTT 
AAAGCTGCTA AGCACTGTAT GACTGACCGT GTTATGCCAG TAAGATGCGC AGCTGCCCAG 
TGTTTACAGG AGATGCTGAA ACATGTACCA TTTGTTTATA CATCTGAACT GGAGACTGTG 
GCTTCCCTGT GTTTTCGATC CTTTGAAGGG TCTAACTACG AAGTTCGATG TGCTGTTGCT 
AATCTGCTTG GAACACTTGT TGCTACCACT CAACAGGCTT CACAGCCTGC AGTGGGTAAA 
AATCGCCTCG CAACATTCGA AGAAGTGTTA GCTTTGATGT CCTCGGGATT TCTTCGTGGT 
GGTATTGGTT TCTTGAAAGG TGGTGCAGGA GAGATGATTA AAGGTAGTTC TAATGTCAGT 
AGAGAAATCA GGGTTGGAGT GACACATGCT TATGTTGTTT TGGTTCGACA ACTTGGAGGC 
TTGTGGTTGG AAAGGAACCT GAATACCCTC TTAGTCCACC TTCTGGAGCT TGTTTCTAGT 
CCTAGAGCAG CAACCTCACA TGTTGATGCC GTTTACAGCC GCAAATGCAT TGCCTTCATC 
TTGCGTTCAG TTTTGGGCAA GATGCTAGGA GAAAAGGCTC AGGCAGCAGC TTGCAAAGAC 
TTAGTACAGA TTATTGCGAA GTATTCAAAT GCAGATGCTG CAGCAGAGTC CTCTGGCAGT 
AAAGAAGGCT TACAAGAAGC AGCCCTTGCT CAACATGTTC TCGTGTGTGC TCTGCAAGAG 
CTTGGTTGTT TAATATCTAG TCTTGGAACC ACAGCCTCTT CGGTAGTGAA TGATTCTTCC 
TCAGGTATAA TTGAAGCTGT GGTATCAGTT TTAATTCACT CAAGTGCAGC ACGACTTGCA 
GCTGCGTGGT GCTTGAGGTC CATTGCAACA GCTGTATCAT CTCAGCTAAC TCCATTGCTA 
GACCGATGTC TAGAGCAACT AGATACCCTT AAAGCGTCAC CCGAGGCTAT TTGTGGATAT 
TCTGGAGCTT TGTCGGCACT TCTTGGAGCA GCATGTTATA CTGCATTAGG CATCCCTCAC 
AGTAAAGGAA AGATCATATT CAATGTAGCG GAGGATCTTC TTCGCACAGC ATCACAGAAT 
AGTAGACTGT CTGTCCAACG TACTCAAGCT GGTTGGTTAA TGATTGGAGC AGTCATGACA 
TTGGGTCCAC CTGTGGTTCG TGGACTTCTG CCAAGAATGT TGCTTCTTTG GAAGAACTCT 
TTTCCAAGGT CAACAAAAGA ACTAGAATCA GAAAAGGCTA GAGGAGATGC TTTCACATGG 
CAAGTAACCC TTGAAGGAAG AGCAGGAGCA TTAGCAGCTA TGGCAAGTTT TCTGGTACAC 
TGTAAAGAAC TTGTCTCTGA AGAGGTGGTG CGTAGATTGC TGTCACCAGT TGAAGCTGCT 
CTTGCCATGC TAACAAGCTT AACAAACCTG GTCAAGTCAT ATGGACAGCA CTTGAAGGCA 
GCTGTTGCGA TGGTGAGACT ACGTCTTTAT GAAGTGCTAA CACTTTTGCC TGCCCAATCA 
TATGAAGGAA GTTACACCAG TCTTTTAAGG CTTTTGGTGG CTGAGTTTAC CCTGACTGAA 
AACCCAGCTA ATACTACTAC ATCGTTGCTT CGAAGCCTTT GCCACACTGA TGATAGTGTG 
ATATTGGGGT CCTGGCTGCA AGAAACTGAT CACAAGGCTA TTGAGGACCA GCTTCAACCA 
AATAGTGCAT CGGGATCAGG TGCATTGGAA CACGATTTGA CAGCTCTGTA TAAGTCACTG 
CCAAAGGGGG AGTCAATTCC AGGTCCTTTA CCATTAGGAG TTGCTGTTAT TGACATGTCT 
GTCATCCTGT TTGGCCAGAC ATTTCCTTAT GTAGCTGTTA AGCACAGACT GCAGATGTTA 
GGTCACTTCT CACAATGTAT TAGGCACACT AAGGCCTCAA GACAGGAAGC TGTTCAAATT 
AACATTTTCA CAGCTGTTCT AAGTGCTCTA AAGATGCTTG CAGAGACAAA AACTCCACTA 
GGACCTGATG ACGTGAGGCA AGCTGCTGCT AGTTTAGTGC TGGGAGCCTT GAGTCACCCA 
AATCCTATAC TACGCTGTGC CGCAGGAGAG GCTTTAGGAC GAATGGCACA GGTTGTGGGT 
GATGGCCGGT TTGTTGCAGA GATGGCTCAG CACAGCTTTG ACAAATTAAA AACGGCCAGA 
GATGCTGTAT CACGTACAGG CCACTCTCTA ACACTAGGAT GTTTACACCG ATACGTTGGG 
GGAATGGGAT CAGGCCAACA TCTTAACACT AGTGTTAGTA TTTTGTTGGC ATTAGCCAAT 
GATACCAATG CTCCTGTTGT TCAGGTATGG GCTCTTCATG CTATAGCCCT CATAGCAGAT 
TCTGGGGGAC CAATGTTCCG TGGATATGTG GAGCCTACCC TCTCTCTAGC TTTAAAATTA 
CTACTGAATA TCCCTCCTTC CAATGTAGAC GTATTGCAGT GTATAGGAAA GAGTCTGTCT 
GCTTTGATTA CTACTGTGGG ACCAGAATTA CAAGGAAATA CGAACTCCAT CAGCACTGCC 
CGTTCTTCCT TTTTGGTAGC CTGTGCCATA ATGCAAGATC ATGGTGACTC CCTAGTCCAT 
GCTGAAGCCA TTTCTTGCCT TCAGCAGTTA CACATGTTTG CTCCACGTCA CGTTAATCTA 
TCAACCCTTG TACCTACCCT TTGTGTGAGT ACATGTGTAT TATACTCTTC CCATCTTCTG 
TTAAGAAGAG CAGCAGTAGC CTGCTTAAGA CAGCTGTCCC AGAGAGAGGC TAAGGAAGTG 
TGTGAACATG CTCTTTCCTG GGCTAGAGAA TCGGGTGGCA CCACAACTGG CTACAACTCT 
AATATCAGCA TTCCTGCAAT AGGAGAGACT GGGCTACCTG GTGTACTCTT TGCCATGCTG 
GACGTAGAGA ATGACCTGAG GCTAGTAGGT CACATCAAGG ACACACTTAC CAGCATGTTA 
CAGGCACTAG CGAGGGAGAA CCTAGTTCAG TGGTTGAGTT TATGTAAAGA TGTTCTTACT 
GCTGCTGATT CTAGTTCATC CAGCATATCA TCTTTGAAAG AAGCTGAGAG AGAATTAGGA 
GATAATGAAG CAGATGTAGA TGACGAGGAG AGATTTAAAG CTTCAGAAGA GCCAGTATCG 
CATCCTTTTG TCTCTCCTCG TTGGCCCACT AGAGTGTTTT CTGCCATTTC TTTGAGGAGG 
ATTATTGAAG CATGTGATGG CAATCGAGCT CATTTTGATT TAGCCATGGC AAGGGAACAG 
AAATTGATTA GACCAAAAGA AGATTTTTTG GTTCTTCATC TGTCTGACCT GATTAGGATG 
GCATTTATGG CAGCTACATC AGAAAGTGAC CCTTTAAGGT TGGAAGGATT AAGAGCATTG 
CAGTTAATTA TTGACAAGTT TTCCAAAGTT CCTGAACCAG AATTTCCAGG TCATGTAATC 
TTGGAACAGT ATCAAGCTCA GGTTGGGGCT GCTCTTCGAC CAGCATTCTC ACCAGAAACA 
CCTTCTCATG TTACTGCTAT GGCTTGTCAG GTGTGTAGTG CCTGGATTGG AAGTGGTGTG 
GCACGAGACT TGAACGATCT TCGACGCGTT CACCAGCTCT TGGTGTCATC TCTGAACAAG 
CTTCATAAAG GGACCAGCTC TCACCTGTAC AATGAGAGTG CTTCTACTCT AGAGAAGCTG 
GCAATATTGA AAGCATGGTC TGAGGTTTTT GTTGTTGCAA TGGAACAGGA AAAACAACTA 
AGCAATGTGC CACCATCCCA AGAGGAACCT CAAAACGATG ATGATGGCAG TTGTGGACAA 
GAAAGTCTCT TAAAGCTTGT AACACCTGAA CTTGTATCTT TAAGCAAGCA CTGGCTTGCT 
GCTTTAAAGG ATCATGCATT ACTCTCACTT CCTGCAGAGT TTTCCAGTCA GTTACCCCAT 
GACGGAGGAG CATTTTACAC CCCTGATACT ATGGATTCTG CTCGACAGTA TTATCGAAAG 
GCCTGGCCAC CCATCCTTCA TGCTGCAGCT CTTTGGCTCT GCACAGGAGG GTTTGAAGGT 
CTTGAACGAG AGTTTGAGCA AGATGCAAAC AACAGCGGAA CAATTTTAGG ACCTCCTGGA 
AGCCCTATGA ACAAAGTTAT TTCAAATCCT AATTCTGAGA GATTTCATCT GCTCTTTGGT 
ATCTGTATGG AGGCACTGTG TAGTCCTCGG TCGGCAGAAA GCCTAGAATG TGTACTTGTC 
TGTTTACAAG CATTACAGCT GCTTTTGAAA CATCCATTGC CAAAAAGCAT TCTTGGCCAG 
GATCCTCAGT TGTGCATTGA ACTGTGTAAT GTTTTACATA GAATTTTGCT TACCCGGGAA 
ACTCCCCGAT GCCAGATGAT TGTGATGAAA ATTGCCCATC TTTTGCTTGA AGCAAGACAT 
GATGACATTC GTTCTCATAA AAAGAAGAAA CTGAAAGAGC TTGCCCCTGC TAATCAAGAA 
CTAGAAGATG TTGACACAGC ATTAGCTGCA CTTGGAGAAG GTGGTGAAAC TGGTGACATA 
GTACCGGGAC AGTCAGTAGT GTTTGCTCTA CTCGAGGTGT GTCTGTGTTT GTTAGTCAGG 
CAGCTCCCTC ATATCAGTCC TGCACTAGCT AACAATACAG GCACGTTTGG CATTCAGGCA 
GCCACACGAT CCAAAAAGTT GACAGAAGAA TCTTCAAAGT TGGTTGCTGC TACTCTGAGT 
GTCATGGCTG AACTTCCAAA CCTTTGTTCG CCCAAAGGTA GCACCACTGT CCTCCCGACC 
ATTCTCTACT TGACAACAGG AGTACTAAAA GAAACAGCTG TTCAGACTAC TGAAGACAGA 
GGATTAGTGG TAGCCTCGCC TCCAGTAAGC ACCACATTGC AAAGTCTGAA ATCTCTCTGC 
ACCTCACCTT TCACAAGGAA CAAACTGTGT GGTTCAGAGT GGCTCACTTT GCTCCAGAGT 
GCTTTAGCTC GGATTTTGGA TCTCAGTAAG ACAAGTTGTG AAGATAGTAA ACCAGATGAA 
ATTTCCATGT TGTTGGCTGT GGCAGTTTTC ATTCTCTCAG CTCCTGGAGA AGTTGTGTCA 
ACACCAAATC TTCAGTTTCC ATGTATCAAT CTTTTTAAAC AAGCACTGCA GCTCGATGGA 
GACCTGGTAC AGCTTAAATG TGTTCAGACA CTCCGTTCCA TGTTTCAACA CCCTGATCGA 
AGTATAGCCA CTCCATACAT CCATACTCTT GCTCCACGAG TCGTGGAAAT ACTTGTGCAG 
TTGACTGCTG ATTTGACAAC TCAGTCTACT GTTACTGATC GCCAGTTTAG TCTAGCCACA 
GAATGTCTGA CCTGTCTAGA CACACTGGTT ACTCTGGCTC CTTCTGATAA AAAAATTCAT 
ATGCTGAGTC TGTACATCCC CCTGGTGGTC AACCTTTTGA TAGAGCCAAC ATTACAACAG 
GGGGCGTCAC ACAACAGGAA GAAGTTACAC GACCATGCTT TGCAACAGCT GATCAAAGTG 
GGCCCTCAAT ATCCCCAGGA CTTCCGCTCC ATCATGACCC AAGCTCCCCA GCTGAGAGGG 
CGGCTTGAAA ATGCCATAAG GAAGGGTTCG CTAATTCACG TTGGAAGCCG AGAATCTTCC 
ACCGCACCTT CGAGTCAAAA AATAAATTCC TCCCAAGCTC CAGCAATCAA ATTAAAGATG 
GATTTTAGCA ATTTCACTGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106466475 ( XM_022394493.1 ) cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab) -> XP_022250201.1 Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), transcript variant X1, mRNA.

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CloneID	OLi98325
Clone ID Related Accession (Same CDS sequence)	XM_022394493.1
Accession Version	XM_022394493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504022400000
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Product	HEAT repeat-containing protein 5B-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013667881.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Limulus polyphemus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAC TATCCCACAG TTTGACTCTA AATGAGGAGG CATTGAGTCA AATAGCTGAA 
GCAAAAAGGC CAGTCTTTGT TTTTGAATGG TTACGATTTT TAGATAAAGT TCTTGGAGCA 
GCTCAGAAGT CAGACATTAA GGAATGCCAG AAAAAACTAG TAGAACAGTT GTTGAATCAA 
GTCCACGAGT CACCAGGACC TCCTACCAGA AGACTAATCG CAAGATGTCT TGCAACACTC 
TTCAGTGTTG GAGATACATT TCTCCTCTTC GAAGCAGTCA ATAGATGTAC AGATATTTTG 
AAAAACAAAG ATGATTCCCC CAGCGTCTTA CCGACAAGAC TGGGGGCAAT TACCTGTATA 
GGAGCCATGT ATGAAAAGCT GGGAAGAATG ATGGGGAGAT CACACGAAGA AGCTGTCACA 
GTTATGCTGA AGTCTCTTAA GAATGCCGAG TCACAGACTC GCTGTGAAAC AATGGTAACT 
ATGGAGAAGA TTATTGCTGG TATGGGAACA GCTGCTGCAT CTGTTCATAA AGATATCTTT 
AAAGCTGCTA AGCACTGTAT GACTGACCGT GTTATGCCAG TAAGATGCGC AGCTGCCCAG 
TGTTTACAGG AGATGCTGAA ACATGTACCA TTTGTTTATA CATCTGAACT GGAGACTGTG 
GCTTCCCTGT GTTTTCGATC CTTTGAAGGG TCTAACTACG AAGTTCGATG TGCTGTTGCT 
AATCTGCTTG GAACACTTGT TGCTACCACT CAACAGGCTT CACAGCCTGC AGTGGGTAAA 
AATCGCCTCG CAACATTCGA AGAAGTGTTA GCTTTGATGT CCTCGGGATT TCTTCGTGGT 
GGTATTGGTT TCTTGAAAGG TGGTGCAGGA GAGATGATTA AAGGTAGTTC TAATGTCAGT 
AGAGAAATCA GGGTTGGAGT GACACATGCT TATGTTGTTT TGGTTCGACA ACTTGGAGGC 
TTGTGGTTGG AAAGGAACCT GAATACCCTC TTAGTCCACC TTCTGGAGCT TGTTTCTAGT 
CCTAGAGCAG CAACCTCACA TGTTGATGCC GTTTACAGCC GCAAATGCAT TGCCTTCATC 
TTGCGTTCAG TTTTGGGCAA GATGCTAGGA GAAAAGGCTC AGGCAGCAGC TTGCAAAGAC 
TTAGTACAGA TTATTGCGAA GTATTCAAAT GCAGATGCTG CAGCAGAGTC CTCTGGCAGT 
AAAGAAGGCT TACAAGAAGC AGCCCTTGCT CAACATGTTC TCGTGTGTGC TCTGCAAGAG 
CTTGGTTGTT TAATATCTAG TCTTGGAACC ACAGCCTCTT CGGTAGTGAA TGATTCTTCC 
TCAGGTATAA TTGAAGCTGT GGTATCAGTT TTAATTCACT CAAGTGCAGC ACGACTTGCA 
GCTGCGTGGT GCTTGAGGTC CATTGCAACA GCTGTATCAT CTCAGCTAAC TCCATTGCTA 
GACCGATGTC TAGAGCAACT AGATACCCTT AAAGCGTCAC CCGAGGCTAT TTGTGGATAT 
TCTGGAGCTT TGTCGGCACT TCTTGGAGCA GCATGTTATA CTGCATTAGG CATCCCTCAC 
AGTAAAGGAA AGATCATATT CAATGTAGCG GAGGATCTTC TTCGCACAGC ATCACAGAAT 
AGTAGACTGT CTGTCCAACG TACTCAAGCT GGTTGGTTAA TGATTGGAGC AGTCATGACA 
TTGGGTCCAC CTGTGGTTCG TGGACTTCTG CCAAGAATGT TGCTTCTTTG GAAGAACTCT 
TTTCCAAGGT CAACAAAAGA ACTAGAATCA GAAAAGGCTA GAGGAGATGC TTTCACATGG 
CAAGTAACCC TTGAAGGAAG AGCAGGAGCA TTAGCAGCTA TGGCAAGTTT TCTGGTACAC 
TGTAAAGAAC TTGTCTCTGA AGAGGTGGTG CGTAGATTGC TGTCACCAGT TGAAGCTGCT 
CTTGCCATGC TAACAAGCTT AACAAACCTG GTCAAGTCAT ATGGACAGCA CTTGAAGGCA 
GCTGTTGCGA TGGTGAGACT ACGTCTTTAT GAAGTGCTAA CACTTTTGCC TGCCCAATCA 
TATGAAGGAA GTTACACCAG TCTTTTAAGG CTTTTGGTGG CTGAGTTTAC CCTGACTGAA 
AACCCAGCTA ATACTACTAC ATCGTTGCTT CGAAGCCTTT GCCACACTGA TGATAGTGTG 
ATATTGGGGT CCTGGCTGCA AGAAACTGAT CACAAGGCTA TTGAGGACCA GATGGAGCCA 
AACAGAAGAT TGGATAGGGA TAATCTTCAA CCAAATAGTG CATCGGGATC AGGTGCATTG 
GAACACGATT TGACAGCTCT GTATAAGTCA CTGCCAAAGG GGGAGTCAAT TCCAGGTCCT 
TTACCATTAG GAGTTGCTGT TATTGACATG TCTGTCATCC TGTTTGGCCA GACATTTCCT 
TATGTAGCTG TTAAGCACAG ACTGCAGATG TTAGGTCACT TCTCACAATG TATTAGGCAC 
ACTAAGGCCT CAAGACAGGA AGCTGTTCAA ATTAACATTT TCACAGCTGT TCTAAGTGCT 
CTAAAGATGC TTGCAGAGAC AAAAACTCCA CTAGGACCTG ATGACGTGAG GCAAGCTGCT 
GCTAGTTTAG TGCTGGGAGC CTTGAGTCAC CCAAATCCTA TACTACGCTG TGCCGCAGGA 
GAGGCTTTAG GACGAATGGC ACAGGTTGTG GGTGATGGCC GGTTTGTTGC AGAGATGGCT 
CAGCACAGCT TTGACAAATT AAAAACGGCC AGAGATGCTG TATCACGTAC AGGCCACTCT 
CTAACACTAG GATGTTTACA CCGATACGTT GGGGGAATGG GATCAGGCCA ACATCTTAAC 
ACTAGTGTTA GTATTTTGTT GGCATTAGCC AATGATACCA ATGCTCCTGT TGTTCAGGTA 
TGGGCTCTTC ATGCTATAGC CCTCATAGCA GATTCTGGGG GACCAATGTT CCGTGGATAT 
GTGGAGCCTA CCCTCTCTCT AGCTTTAAAA TTACTACTGA ATATCCCTCC TTCCAATGTA 
GACGTATTGC AGTGTATAGG AAAGAGTCTG TCTGCTTTGA TTACTACTGT GGGACCAGAA 
TTACAAGGAA ATACGAACTC CATCAGCACT GCCCGTTCTT CCTTTTTGGT AGCCTGTGCC 
ATAATGCAAG ATCATGGTGA CTCCCTAGTC CATGCTGAAG CCATTTCTTG CCTTCAGCAG 
TTACACATGT TTGCTCCACG TCACGTTAAT CTATCAACCC TTGTACCTAC CCTTTGTTGT 
GTATTATACT CTTCCCATCT TCTGTTAAGA AGAGCAGCAG TAGCCTGCTT AAGACAGCTG 
TCCCAGAGAG AGGCTAAGGA AGTGTGTGAA CATGCTCTTT CCTGGGCTAG AGAATCGGGT 
GGCACCACAA CTGGCTACAA CTCTAATATC AGCATTCCTG CAATAGGAGA GACTGGGCTA 
CCTGGTGTAC TCTTTGCCAT GCTGGACGTA GAGAATGACC TGAGGCTAGT AGGTCACATC 
AAGGACACAC TTACCAGCAT GTTACAGGCA CTAGCGAGGG AGAACCTAGT TCAGTGGTTG 
AGTTTATGTA AAGATGTTCT TACTGCTGCT GATTCTAGTT CATCCAGCAT ATCATCTTTG 
AAAGAAGCTG AGAGAGAATT AGGAGATAAT GAAGCAGATG TAGATGACGA GGAGAGATTT 
AAAGCTTCAG AAGAGCCAGT ATCGCATCCT TTTGTCTCTC CTCGTTGGCC CACTAGAGTG 
TTTTCTGCCA TTTCTTTGAG GAGGATTATT GAAGCATGTG ATGGCAATCG AGCTCATTTT 
GATTTAGCCA TGGCAAGGGA ACAGAAATTG ATTAGACCAA AAGAAGATTT TTTGGTTCTT 
CATCTGTCTG ACCTGATTAG GATGGCATTT ATGGCAGCTA CATCAGAAAG TGACCCTTTA 
AGGTTGGAAG GATTAAGAGC ATTGCAGTTA ATTATTGACA AGTTTTCCAA AGTTCCTGAA 
CCAGAATTTC CAGGTCATGT AATCTTGGAA CAGTATCAAG CTCAGGTTGG GGCTGCTCTT 
CGACCAGCAT TCTCACCAGA AACACCTTCT CATGTTACTG CTATGGCTTG TCAGGTGTGT 
AGTGCCTGGA TTGGAAGTGG TGTGGCACGA GACTTGAACG ATCTTCGACG CGTTCACCAG 
CTCTTGGTGT CATCTCTGAA CAAGCTTCAT AAAGGGACCA GCTCTCACCT GTACAATGAG 
AGTGCTTCTA CTCTAGAGAA GCTGGCAATA TTGAAAGCAT GGTCTGAGGT TTTTGTTGTT 
GCAATGGAAC AGGAAAAACA ACTAAGCAAT GTGCCACCAT CCCAAGAGGA ACCTCAAAAC 
GATGATGATG GCAGTTGTGG ACAAGAAAGT CTCTTAAAGC TTGTAACACC TGAACTTGTA 
TCTTTAAGCA AGCACTGGCT TGCTGCTTTA AAGGATCATG CATTACTCTC ACTTCCTGCA 
GAGTTTTCCA GTCAGTTACC CCATGACGGA GGAGCATTTT ACACCCCTGA TACTATGGAT 
TCTGCTCGAC AGTATTATCG AAAGGCCTGG CCACCCATCC TTCATGCTGC AGCTCTTTGG 
CTCTGCACAG GAGGGTTTGA AGGTCTTGAA CGAGAGTTTG AGCAAGATGC AAACAACAGC 
GGAACAATTT TAGGACCTCC TGGAAGCCCT ATGAACAAAG TTATTTCAAA TCCTAATTCT 
GAGAGATTTC ATCTGCTCTT TGGTATCTGT ATGGAGGCAC TGTGTAGTCC TCGGTCGGCA 
GAAAGCCTAG AATGTGTACT TGTCTGTTTA CAAGCATTAC AGCTGCTTTT GAAACATCCA 
TTGCCAAAAA GCATTCTTGG CCAGGATCCT CAGTTGTGCA TTGAACTGTG TAATGTTTTA 
CATAGAATTT TGCTTACCCG GGAAACTCCC CGATGCCAGA TGATTGTGAT GAAAATTGCC 
CATCTTTTGC TTGAAGCAAG ACATGATGAC ATTCGTTCTC ATAAAAAGAA GAAACTGAAA 
GAGCTTGCCC CTGCTAATCA AGAACTAGAA GATGTTGACA CAGCATTAGC TGCACTTGGA 
GAAGGTGGTG AAACTGGTGA CATAGTACCG GGACAGTCAG TAGTGTTTGC TCTACTCGAG 
GTGTGTCTGT GTTTGTTAGT CAGGCAGCTC CCTCATATCA GTCCTGCACT AGCTAACAAT 
ACAGGCACGT TTGGCATTCA GGCAGCCACA CGATCCAAAA AGTTGACAGA AGAATCTTCA 
AAGTTGGTTG CTGCTACTCT GAGTGTCATG GCTGAACTTC CAAACCTTTG TTCGCCCAAA 
GGTAGCACCA CTGTCCTCCC GACCATTCTC TACTTGACAA CAGGAGTACT AAAAGAAACA 
GCTGTTCAGA CTACTGAAGA CAGAGGATTA GTGGTAGCCT CGCCTCCAGT AAGCACCACA 
TTGCAAAGTC TGAAATCTCT CTGCACCTCA CCTTTCACAA GGAACAAACT GTGTGGTTCA 
GAGTGGCTCA CTTTGCTCCA GAGTGCTTTA GCTCGGATTT TGGATCTCAG TAAGACAAGT 
TGTGAAGATA GTAAACCAGA TGAAATTTCC ATGTTGTTGG CTGTGGCAGT TTTCATTCTC 
TCAGCTCCTG GAGAAGTTGT GTCAACACCA AATCTTCAGT TTCCATGTAT CAATCTTTTT 
AAACAAGCAC TGCAGCTCGA TGGAGACCTG GTACAGCTTA AATGTGTTCA GACACTCCGT 
TCCATGTTTC AACACCCTGA TCGAAGTATA GCCACTCCAT ACATCCATAC TCTTGCTCCA 
CGAGTCGTGG AAATACTTGT GCAGTTGACT GCTGATTTGA CAACTCAGTC TACTGTTACT 
GATCGCCAGT TTAGTCTAGC CACAGAATGT CTGACCTGTC TAGACACACT GGTTACTCTG 
GCTCCTTCTG ATAAAAAAAT TCATATGCTG AGTCTGTACA TCCCCCTGGT GGTCAACCTT 
TTGATAGAGC CAACATTACA ACAGGGGGCG TCACACAACA GGAAGAAGTT ACACGACCAT 
GCTTTGCAAC AGCTGATCAA AGTGGGCCCT CAATATCCCC AGGACTTCCG CTCCATCATG 
ACCCAAGCTC CCCAGCTGAG AGGGCGGCTT GAAAATGCCA TAAGGAAGGG TTCGCTAATT 
CACGTTGGAA GCCGAGAATC TTCCACCGCA CCTTCGAGTC AAAAAATAAA TTCCTCCCAA 
GCTCCAGCAA TCAAATTAAA GATGGATTTT AGCAATTTCA CTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022250201.1
CDS	202..6429
Translation	MMELSHSLTLNEEALSQIAEAKRPVFVFEWLRFLDKVLGAAQKSDIKECQKKLVEQLLNQVHESPGPPTRRLIARCLATLFSVGDTFLLFEAVNRCTDILKNKDDSPSVLPTRLGAITCIGAMYEKLGRMMGRSHEEAVTVMLKSLKNAESQTRCETMVTMEKIIAGMGTAAASVHKDIFKAAKHCMTDRVMPVRCAAAQCLQEMLKHVPFVYTSELETVASLCFRSFEGSNYEVRCAVANLLGTLVATTQQASQPAVGKNRLATFEEVLALMSSGFLRGGIGFLKGGAGEMIKGSSNVSREIRVGVTHAYVVLVRQLGGLWLERNLNTLLVHLLELVSSPRAATSHVDAVYSRKCIAFILRSVLGKMLGEKAQAAACKDLVQIIAKYSNADAAAESSGSKEGLQEAALAQHVLVCALQELGCLISSLGTTASSVVNDSSSGIIEAVVSVLIHSSAARLAAAWCLRSIATAVSSQLTPLLDRCLEQLDTLKASPEAICGYSGALSALLGAACYTALGIPHSKGKIIFNVAEDLLRTASQNSRLSVQRTQAGWLMIGAVMTLGPPVVRGLLPRMLLLWKNSFPRSTKELESEKARGDAFTWQVTLEGRAGALAAMASFLVHCKELVSEEVVRRLLSPVEAALAMLTSLTNLVKSYGQHLKAAVAMVRLRLYEVLTLLPAQSYEGSYTSLLRLLVAEFTLTENPANTTTSLLRSLCHTDDSVILGSWLQETDHKAIEDQMEPNRRLDRDNLQPNSASGSGALEHDLTALYKSLPKGESIPGPLPLGVAVIDMSVILFGQTFPYVAVKHRLQMLGHFSQCIRHTKASRQEAVQINIFTAVLSALKMLAETKTPLGPDDVRQAAASLVLGALSHPNPILRCAAGEALGRMAQVVGDGRFVAEMAQHSFDKLKTARDAVSRTGHSLTLGCLHRYVGGMGSGQHLNTSVSILLALANDTNAPVVQVWALHAIALIADSGGPMFRGYVEPTLSLALKLLLNIPPSNVDVLQCIGKSLSALITTVGPELQGNTNSISTARSSFLVACAIMQDHGDSLVHAEAISCLQQLHMFAPRHVNLSTLVPTLCCVLYSSHLLLRRAAVACLRQLSQREAKEVCEHALSWARESGGTTTGYNSNISIPAIGETGLPGVLFAMLDVENDLRLVGHIKDTLTSMLQALARENLVQWLSLCKDVLTAADSSSSSISSLKEAERELGDNEADVDDEERFKASEEPVSHPFVSPRWPTRVFSAISLRRIIEACDGNRAHFDLAMAREQKLIRPKEDFLVLHLSDLIRMAFMAATSESDPLRLEGLRALQLIIDKFSKVPEPEFPGHVILEQYQAQVGAALRPAFSPETPSHVTAMACQVCSAWIGSGVARDLNDLRRVHQLLVSSLNKLHKGTSSHLYNESASTLEKLAILKAWSEVFVVAMEQEKQLSNVPPSQEEPQNDDDGSCGQESLLKLVTPELVSLSKHWLAALKDHALLSLPAEFSSQLPHDGGAFYTPDTMDSARQYYRKAWPPILHAAALWLCTGGFEGLEREFEQDANNSGTILGPPGSPMNKVISNPNSERFHLLFGICMEALCSPRSAESLECVLVCLQALQLLLKHPLPKSILGQDPQLCIELCNVLHRILLTRETPRCQMIVMKIAHLLLEARHDDIRSHKKKKLKELAPANQELEDVDTALAALGEGGETGDIVPGQSVVFALLEVCLCLLVRQLPHISPALANNTGTFGIQAATRSKKLTEESSKLVAATLSVMAELPNLCSPKGSTTVLPTILYLTTGVLKETAVQTTEDRGLVVASPPVSTTLQSLKSLCTSPFTRNKLCGSEWLTLLQSALARILDLSKTSCEDSKPDEISMLLAVAVFILSAPGEVVSTPNLQFPCINLFKQALQLDGDLVQLKCVQTLRSMFQHPDRSIATPYIHTLAPRVVEILVQLTADLTTQSTVTDRQFSLATECLTCLDTLVTLAPSDKKIHMLSLYIPLVVNLLIEPTLQQGASHNRKKLHDHALQQLIKVGPQYPQDFRSIMTQAPQLRGRLENAIRKGSLIHVGSRESSTAPSSQKINSSQAPAIKLKMDFSNFTG

Target ORF information:

RefSeq Version	XM_022394493.1
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Definition	Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022394493.1

ORF Insert Sequence:

ATGATGGAAC TATCCCACAG TTTGACTCTA AATGAGGAGG CATTGAGTCA AATAGCTGAA 
GCAAAAAGGC CAGTCTTTGT TTTTGAATGG TTACGATTTT TAGATAAAGT TCTTGGAGCA 
GCTCAGAAGT CAGACATTAA GGAATGCCAG AAAAAACTAG TAGAACAGTT GTTGAATCAA 
GTCCACGAGT CACCAGGACC TCCTACCAGA AGACTAATCG CAAGATGTCT TGCAACACTC 
TTCAGTGTTG GAGATACATT TCTCCTCTTC GAAGCAGTCA ATAGATGTAC AGATATTTTG 
AAAAACAAAG ATGATTCCCC CAGCGTCTTA CCGACAAGAC TGGGGGCAAT TACCTGTATA 
GGAGCCATGT ATGAAAAGCT GGGAAGAATG ATGGGGAGAT CACACGAAGA AGCTGTCACA 
GTTATGCTGA AGTCTCTTAA GAATGCCGAG TCACAGACTC GCTGTGAAAC AATGGTAACT 
ATGGAGAAGA TTATTGCTGG TATGGGAACA GCTGCTGCAT CTGTTCATAA AGATATCTTT 
AAAGCTGCTA AGCACTGTAT GACTGACCGT GTTATGCCAG TAAGATGCGC AGCTGCCCAG 
TGTTTACAGG AGATGCTGAA ACATGTACCA TTTGTTTATA CATCTGAACT GGAGACTGTG 
GCTTCCCTGT GTTTTCGATC CTTTGAAGGG TCTAACTACG AAGTTCGATG TGCTGTTGCT 
AATCTGCTTG GAACACTTGT TGCTACCACT CAACAGGCTT CACAGCCTGC AGTGGGTAAA 
AATCGCCTCG CAACATTCGA AGAAGTGTTA GCTTTGATGT CCTCGGGATT TCTTCGTGGT 
GGTATTGGTT TCTTGAAAGG TGGTGCAGGA GAGATGATTA AAGGTAGTTC TAATGTCAGT 
AGAGAAATCA GGGTTGGAGT GACACATGCT TATGTTGTTT TGGTTCGACA ACTTGGAGGC 
TTGTGGTTGG AAAGGAACCT GAATACCCTC TTAGTCCACC TTCTGGAGCT TGTTTCTAGT 
CCTAGAGCAG CAACCTCACA TGTTGATGCC GTTTACAGCC GCAAATGCAT TGCCTTCATC 
TTGCGTTCAG TTTTGGGCAA GATGCTAGGA GAAAAGGCTC AGGCAGCAGC TTGCAAAGAC 
TTAGTACAGA TTATTGCGAA GTATTCAAAT GCAGATGCTG CAGCAGAGTC CTCTGGCAGT 
AAAGAAGGCT TACAAGAAGC AGCCCTTGCT CAACATGTTC TCGTGTGTGC TCTGCAAGAG 
CTTGGTTGTT TAATATCTAG TCTTGGAACC ACAGCCTCTT CGGTAGTGAA TGATTCTTCC 
TCAGGTATAA TTGAAGCTGT GGTATCAGTT TTAATTCACT CAAGTGCAGC ACGACTTGCA 
GCTGCGTGGT GCTTGAGGTC CATTGCAACA GCTGTATCAT CTCAGCTAAC TCCATTGCTA 
GACCGATGTC TAGAGCAACT AGATACCCTT AAAGCGTCAC CCGAGGCTAT TTGTGGATAT 
TCTGGAGCTT TGTCGGCACT TCTTGGAGCA GCATGTTATA CTGCATTAGG CATCCCTCAC 
AGTAAAGGAA AGATCATATT CAATGTAGCG GAGGATCTTC TTCGCACAGC ATCACAGAAT 
AGTAGACTGT CTGTCCAACG TACTCAAGCT GGTTGGTTAA TGATTGGAGC AGTCATGACA 
TTGGGTCCAC CTGTGGTTCG TGGACTTCTG CCAAGAATGT TGCTTCTTTG GAAGAACTCT 
TTTCCAAGGT CAACAAAAGA ACTAGAATCA GAAAAGGCTA GAGGAGATGC TTTCACATGG 
CAAGTAACCC TTGAAGGAAG AGCAGGAGCA TTAGCAGCTA TGGCAAGTTT TCTGGTACAC 
TGTAAAGAAC TTGTCTCTGA AGAGGTGGTG CGTAGATTGC TGTCACCAGT TGAAGCTGCT 
CTTGCCATGC TAACAAGCTT AACAAACCTG GTCAAGTCAT ATGGACAGCA CTTGAAGGCA 
GCTGTTGCGA TGGTGAGACT ACGTCTTTAT GAAGTGCTAA CACTTTTGCC TGCCCAATCA 
TATGAAGGAA GTTACACCAG TCTTTTAAGG CTTTTGGTGG CTGAGTTTAC CCTGACTGAA 
AACCCAGCTA ATACTACTAC ATCGTTGCTT CGAAGCCTTT GCCACACTGA TGATAGTGTG 
ATATTGGGGT CCTGGCTGCA AGAAACTGAT CACAAGGCTA TTGAGGACCA GATGGAGCCA 
AACAGAAGAT TGGATAGGGA TAATCTTCAA CCAAATAGTG CATCGGGATC AGGTGCATTG 
GAACACGATT TGACAGCTCT GTATAAGTCA CTGCCAAAGG GGGAGTCAAT TCCAGGTCCT 
TTACCATTAG GAGTTGCTGT TATTGACATG TCTGTCATCC TGTTTGGCCA GACATTTCCT 
TATGTAGCTG TTAAGCACAG ACTGCAGATG TTAGGTCACT TCTCACAATG TATTAGGCAC 
ACTAAGGCCT CAAGACAGGA AGCTGTTCAA ATTAACATTT TCACAGCTGT TCTAAGTGCT 
CTAAAGATGC TTGCAGAGAC AAAAACTCCA CTAGGACCTG ATGACGTGAG GCAAGCTGCT 
GCTAGTTTAG TGCTGGGAGC CTTGAGTCAC CCAAATCCTA TACTACGCTG TGCCGCAGGA 
GAGGCTTTAG GACGAATGGC ACAGGTTGTG GGTGATGGCC GGTTTGTTGC AGAGATGGCT 
CAGCACAGCT TTGACAAATT AAAAACGGCC AGAGATGCTG TATCACGTAC AGGCCACTCT 
CTAACACTAG GATGTTTACA CCGATACGTT GGGGGAATGG GATCAGGCCA ACATCTTAAC 
ACTAGTGTTA GTATTTTGTT GGCATTAGCC AATGATACCA ATGCTCCTGT TGTTCAGGTA 
TGGGCTCTTC ATGCTATAGC CCTCATAGCA GATTCTGGGG GACCAATGTT CCGTGGATAT 
GTGGAGCCTA CCCTCTCTCT AGCTTTAAAA TTACTACTGA ATATCCCTCC TTCCAATGTA 
GACGTATTGC AGTGTATAGG AAAGAGTCTG TCTGCTTTGA TTACTACTGT GGGACCAGAA 
TTACAAGGAA ATACGAACTC CATCAGCACT GCCCGTTCTT CCTTTTTGGT AGCCTGTGCC 
ATAATGCAAG ATCATGGTGA CTCCCTAGTC CATGCTGAAG CCATTTCTTG CCTTCAGCAG 
TTACACATGT TTGCTCCACG TCACGTTAAT CTATCAACCC TTGTACCTAC CCTTTGTTGT 
GTATTATACT CTTCCCATCT TCTGTTAAGA AGAGCAGCAG TAGCCTGCTT AAGACAGCTG 
TCCCAGAGAG AGGCTAAGGA AGTGTGTGAA CATGCTCTTT CCTGGGCTAG AGAATCGGGT 
GGCACCACAA CTGGCTACAA CTCTAATATC AGCATTCCTG CAATAGGAGA GACTGGGCTA 
CCTGGTGTAC TCTTTGCCAT GCTGGACGTA GAGAATGACC TGAGGCTAGT AGGTCACATC 
AAGGACACAC TTACCAGCAT GTTACAGGCA CTAGCGAGGG AGAACCTAGT TCAGTGGTTG 
AGTTTATGTA AAGATGTTCT TACTGCTGCT GATTCTAGTT CATCCAGCAT ATCATCTTTG 
AAAGAAGCTG AGAGAGAATT AGGAGATAAT GAAGCAGATG TAGATGACGA GGAGAGATTT 
AAAGCTTCAG AAGAGCCAGT ATCGCATCCT TTTGTCTCTC CTCGTTGGCC CACTAGAGTG 
TTTTCTGCCA TTTCTTTGAG GAGGATTATT GAAGCATGTG ATGGCAATCG AGCTCATTTT 
GATTTAGCCA TGGCAAGGGA ACAGAAATTG ATTAGACCAA AAGAAGATTT TTTGGTTCTT 
CATCTGTCTG ACCTGATTAG GATGGCATTT ATGGCAGCTA CATCAGAAAG TGACCCTTTA 
AGGTTGGAAG GATTAAGAGC ATTGCAGTTA ATTATTGACA AGTTTTCCAA AGTTCCTGAA 
CCAGAATTTC CAGGTCATGT AATCTTGGAA CAGTATCAAG CTCAGGTTGG GGCTGCTCTT 
CGACCAGCAT TCTCACCAGA AACACCTTCT CATGTTACTG CTATGGCTTG TCAGGTGTGT 
AGTGCCTGGA TTGGAAGTGG TGTGGCACGA GACTTGAACG ATCTTCGACG CGTTCACCAG 
CTCTTGGTGT CATCTCTGAA CAAGCTTCAT AAAGGGACCA GCTCTCACCT GTACAATGAG 
AGTGCTTCTA CTCTAGAGAA GCTGGCAATA TTGAAAGCAT GGTCTGAGGT TTTTGTTGTT 
GCAATGGAAC AGGAAAAACA ACTAAGCAAT GTGCCACCAT CCCAAGAGGA ACCTCAAAAC 
GATGATGATG GCAGTTGTGG ACAAGAAAGT CTCTTAAAGC TTGTAACACC TGAACTTGTA 
TCTTTAAGCA AGCACTGGCT TGCTGCTTTA AAGGATCATG CATTACTCTC ACTTCCTGCA 
GAGTTTTCCA GTCAGTTACC CCATGACGGA GGAGCATTTT ACACCCCTGA TACTATGGAT 
TCTGCTCGAC AGTATTATCG AAAGGCCTGG CCACCCATCC TTCATGCTGC AGCTCTTTGG 
CTCTGCACAG GAGGGTTTGA AGGTCTTGAA CGAGAGTTTG AGCAAGATGC AAACAACAGC 
GGAACAATTT TAGGACCTCC TGGAAGCCCT ATGAACAAAG TTATTTCAAA TCCTAATTCT 
GAGAGATTTC ATCTGCTCTT TGGTATCTGT ATGGAGGCAC TGTGTAGTCC TCGGTCGGCA 
GAAAGCCTAG AATGTGTACT TGTCTGTTTA CAAGCATTAC AGCTGCTTTT GAAACATCCA 
TTGCCAAAAA GCATTCTTGG CCAGGATCCT CAGTTGTGCA TTGAACTGTG TAATGTTTTA 
CATAGAATTT TGCTTACCCG GGAAACTCCC CGATGCCAGA TGATTGTGAT GAAAATTGCC 
CATCTTTTGC TTGAAGCAAG ACATGATGAC ATTCGTTCTC ATAAAAAGAA GAAACTGAAA 
GAGCTTGCCC CTGCTAATCA AGAACTAGAA GATGTTGACA CAGCATTAGC TGCACTTGGA 
GAAGGTGGTG AAACTGGTGA CATAGTACCG GGACAGTCAG TAGTGTTTGC TCTACTCGAG 
GTGTGTCTGT GTTTGTTAGT CAGGCAGCTC CCTCATATCA GTCCTGCACT AGCTAACAAT 
ACAGGCACGT TTGGCATTCA GGCAGCCACA CGATCCAAAA AGTTGACAGA AGAATCTTCA 
AAGTTGGTTG CTGCTACTCT GAGTGTCATG GCTGAACTTC CAAACCTTTG TTCGCCCAAA 
GGTAGCACCA CTGTCCTCCC GACCATTCTC TACTTGACAA CAGGAGTACT AAAAGAAACA 
GCTGTTCAGA CTACTGAAGA CAGAGGATTA GTGGTAGCCT CGCCTCCAGT AAGCACCACA 
TTGCAAAGTC TGAAATCTCT CTGCACCTCA CCTTTCACAA GGAACAAACT GTGTGGTTCA 
GAGTGGCTCA CTTTGCTCCA GAGTGCTTTA GCTCGGATTT TGGATCTCAG TAAGACAAGT 
TGTGAAGATA GTAAACCAGA TGAAATTTCC ATGTTGTTGG CTGTGGCAGT TTTCATTCTC 
TCAGCTCCTG GAGAAGTTGT GTCAACACCA AATCTTCAGT TTCCATGTAT CAATCTTTTT 
AAACAAGCAC TGCAGCTCGA TGGAGACCTG GTACAGCTTA AATGTGTTCA GACACTCCGT 
TCCATGTTTC AACACCCTGA TCGAAGTATA GCCACTCCAT ACATCCATAC TCTTGCTCCA 
CGAGTCGTGG AAATACTTGT GCAGTTGACT GCTGATTTGA CAACTCAGTC TACTGTTACT 
GATCGCCAGT TTAGTCTAGC CACAGAATGT CTGACCTGTC TAGACACACT GGTTACTCTG 
GCTCCTTCTG ATAAAAAAAT TCATATGCTG AGTCTGTACA TCCCCCTGGT GGTCAACCTT 
TTGATAGAGC CAACATTACA ACAGGGGGCG TCACACAACA GGAAGAAGTT ACACGACCAT 
GCTTTGCAAC AGCTGATCAA AGTGGGCCCT CAATATCCCC AGGACTTCCG CTCCATCATG 
ACCCAAGCTC CCCAGCTGAG AGGGCGGCTT GAAAATGCCA TAAGGAAGGG TTCGCTAATT 
CACGTTGGAA GCCGAGAATC TTCCACCGCA CCTTCGAGTC AAAAAATAAA TTCCTCCCAA 
GCTCCAGCAA TCAAATTAAA GATGGATTTT AGCAATTTCA CTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106466475 ( XM_022394494.1 ) cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab) -> XP_022250202.1 Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), transcript variant X2, mRNA.

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CloneID	OLi98326
Clone ID Related Accession (Same CDS sequence)	XM_022394494.1
Accession Version	XM_022394494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504022400000
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Product	HEAT repeat-containing protein 5B-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013667881.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Limulus polyphemus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAC TATCCCACAG TTTGACTCTA AATGAGGAGG CATTGAGTCA AATAGCTGAA 
GCAAAAAGGC CAGTCTTTGT TTTTGAATGG TTACGATTTT TAGATAAAGT TCTTGGAGCA 
GCTCAGAAGT CAGACATTAA GGAATGCCAG AAAAAACTAG TAGAACAGTT GTTGAATCAA 
GTCCACGAGT CACCAGGACC TCCTACCAGA AGACTAATCG CAAGATGTCT TGCAACACTC 
TTCAGTGTTG GAGATACATT TCTCCTCTTC GAAGCAGTCA ATAGATGTAC AGATATTTTG 
AAAAACAAAG ATGATTCCCC CAGCGTCTTA CCGACAAGAC TGGGGGCAAT TACCTGTATA 
GGAGCCATGT ATGAAAAGCT GGGAAGAATG ATGGGGAGAT CACACGAAGA AGCTGTCACA 
GTTATGCTGA AGTCTCTTAA GAATGCCGAG TCACAGACTC GCTGTGAAAC AATGGTAACT 
ATGGAGAAGA TTATTGCTGG TATGGGAACA GCTGCTGCAT CTGTTCATAA AGATATCTTT 
AAAGCTGCTA AGCACTGTAT GACTGACCGT GTTATGCCAG TAAGATGCGC AGCTGCCCAG 
TGTTTACAGG AGATGCTGAA ACATGTACCA TTTGTTTATA CATCTGAACT GGAGACTGTG 
GCTTCCCTGT GTTTTCGATC CTTTGAAGGG TCTAACTACG AAGTTCGATG TGCTGTTGCT 
AATCTGCTTG GAACACTTGT TGCTACCACT CAACAGGCTT CACAGCCTGC AGTGGGTAAA 
AATCGCCTCG CAACATTCGA AGAAGTGTTA GCTTTGATGT CCTCGGGATT TCTTCGTGGT 
GGTATTGGTT TCTTGAAAGG TGGTGCAGGA GAGATGATTA AAGGTAGTTC TAATGTCAGT 
AGAGAAATCA GGGTTGGAGT GACACATGCT TATGTTGTTT TGGTTCGACA ACTTGGAGGC 
TTGTGGTTGG AAAGGAACCT GAATACCCTC TTAGTCCACC TTCTGGAGCT TGTTTCTAGT 
CCTAGAGCAG CAACCTCACA TGTTGATGCC GTTTACAGCC GCAAATGCAT TGCCTTCATC 
TTGCGTTCAG TTTTGGGCAA GATGCTAGGA GAAAAGGCTC AGGCAGCAGC TTGCAAAGAC 
TTAGTACAGA TTATTGCGAA GTATTCAAAT GCAGATGCTG CAGCAGAGTC CTCTGGCAGT 
AAAGAAGGCT TACAAGAAGC AGCCCTTGCT CAACATGTTC TCGTGTGTGC TCTGCAAGAG 
CTTGGTTGTT TAATATCTAG TCTTGGAACC ACAGCCTCTT CGGTAGTGAA TGATTCTTCC 
TCAGGTATAA TTGAAGCTGT GGTATCAGTT TTAATTCACT CAAGTGCAGC ACGACTTGCA 
GCTGCGTGGT GCTTGAGGTC CATTGCAACA GCTGTATCAT CTCAGCTAAC TCCATTGCTA 
GACCGATGTC TAGAGCAACT AGATACCCTT AAAGCGTCAC CCGAGGCTAT TTGTGGATAT 
TCTGGAGCTT TGTCGGCACT TCTTGGAGCA GCATGTTATA CTGCATTAGG CATCCCTCAC 
AGTAAAGGAA AGATCATATT CAATGTAGCG GAGGATCTTC TTCGCACAGC ATCACAGAAT 
AGTAGACTGT CTGTCCAACG TACTCAAGCT GGTTGGTTAA TGATTGGAGC AGTCATGACA 
TTGGGTCCAC CTGTGGTTCG TGGACTTCTG CCAAGAATGT TGCTTCTTTG GAAGAACTCT 
TTTCCAAGGT CAACAAAAGA ACTAGAATCA GAAAAGGCTA GAGGAGATGC TTTCACATGG 
CAAGTAACCC TTGAAGGAAG AGCAGGAGCA TTAGCAGCTA TGGCAAGTTT TCTGGTACAC 
TGTAAAGAAC TTGTCTCTGA AGAGGTGGTG CGTAGATTGC TGTCACCAGT TGAAGCTGCT 
CTTGCCATGC TAACAAGCTT AACAAACCTG GTCAAGTCAT ATGGACAGCA CTTGAAGGCA 
GCTGTTGCGA TGGTGAGACT ACGTCTTTAT GAAGTGCTAA CACTTTTGCC TGCCCAATCA 
TATGAAGGAA GTTACACCAG TCTTTTAAGG CTTTTGGTGG CTGAGTTTAC CCTGACTGAA 
AACCCAGCTA ATACTACTAC ATCGTTGCTT CGAAGCCTTT GCCACACTGA TGATAGTGTG 
ATATTGGGGT CCTGGCTGCA AGAAACTGAT CACAAGGCTA TTGAGGACCA GATGGAGCCA 
AACAGAAGAT TGGATAGGGA TAATCTTCAA CCAAATAGTG CATCGGGATC AGGTGCATTG 
GAACACGATT TGACAGCTCT GTATAAGTCA CTGCCAAAGG GGGAGTCAAT TCCAGGTCCT 
TTACCATTAG GAGTTGCTGT TATTGACATG TCTGTCATCC TGTTTGGCCA GACATTTCCT 
TATGTAGCTG TTAAGCACAG ACTGCAGATG TTAGGTCACT TCTCACAATG TATTAGGCAC 
ACTAAGGCCT CAAGACAGGA AGCTGTTCAA ATTAACATTT TCACAGCTGT TCTAAGTGCT 
CTAAAGATGC TTGCAGAGAC AAAAACTCCA CTAGGACCTG ATGACGTGAG GCAAGCTGCT 
GCTAGTTTAG TGCTGGGAGC CTTGAGTCAC CCAAATCCTA TACTACGCTG TGCCGCAGGA 
GAGGCTTTAG GACGAATGGC ACAGGTTGTG GGTGATGGCC GGTTTGTTGC AGAGATGGCT 
CAGCACAGCT TTGACAAATT AAAAACGGCC AGAGATGCTG TATCACGTAC AGGCCACTCT 
CTAACACTAG GATGTTTACA CCGATACGTT GGGGGAATGG GATCAGGCCA ACATCTTAAC 
ACTAGTGTTA GTATTTTGTT GGCATTAGCC AATGATACCA ATGCTCCTGT TGTTCAGGTA 
TGGGCTCTTC ATGCTATAGC CCTCATAGCA GATTCTGGGG GACCAATGTT CCGTGGATAT 
GTGGAGCCTA CCCTCTCTCT AGCTTTAAAA TTACTACTGA ATATCCCTCC TTCCAATGTA 
GACGTATTGC AGTGTATAGG AAAGAGTCTG TCTGCTTTGA TTACTACTGT GGGACCAGAA 
TTACAAGGAA ATACGAACTC CATCAGCACT GCCCGTTCTT CCTTTTTGGT AGCCTGTGCC 
ATAATGCAAG ATCATGGTGA CTCCCTAGTC CATGCTGAAG CCATTTCTTG CCTTCAGCAG 
TTACACATGT TTGCTCCACG TCACGTTAAT CTATCAACCC TTGTACCTAC CCTTTGTTGT 
GTATTATACT CTTCCCATCT TCTGTTAAGA AGAGCAGCAG TAGCCTGCTT AAGACAGCTG 
TCCCAGAGAG AGGCTAAGGA AGTGTGTGAA CATGCTCTTT CCTGGGCTAG AGAATCGGGT 
GGCACCACAA CTGGCTACAA CTCTAATATC AGCATTCCTG CAATAGGAGA GACTGGGCTA 
CCTGGTGTAC TCTTTGCCAT GCTGGACGTA GAGAATGACC TGAGGCTAGT AGGTCACATC 
AAGGACACAC TTACCAGCAT GTTACAGGCA CTAGCGAGGG AGAACCTAGT TCAGTGGTTG 
AGTTTATGTA AAGATGTTCT TACTGCTGCT GATTCTAGTT CATCCAGCAT ATCATCTTTG 
AAAGAAGCTG AGAGAGAATT AGGAGATAAT GAAGCAGATG TAGATGACGA GGAGAGATTT 
AAAGCTTCAG AAGAGCCAGT ATCGCATCCT TTTGTCTCTC CTCGTTGGCC CACTAGAGTG 
TTTTCTGCCA TTTCTTTGAG GAGGATTATT GAAGCATGTG ATGGCAATCG AGCTCATTTT 
GATTTAGCCA TGGCAAGGGA ACAGAAATTG ATTAGACCAA AAGAAGATTT TTTGGTTCTT 
CATCTGTCTG ACCTGATTAG GATGGCATTT ATGGCAGCTA CATCAGAAAG TGACCCTTTA 
AGGTTGGAAG GATTAAGAGC ATTGCAGTTA ATTATTGACA AGTTTTCCAA AGTTCCTGAA 
CCAGAATTTC CAGGTCATGT AATCTTGGAA CAGTATCAAG CTCAGGTTGG GGCTGCTCTT 
CGACCAGCAT TCTCACCAGA AACACCTTCT CATGTTACTG CTATGGCTTG TCAGGTGTGT 
AGTGCCTGGA TTGGAAGTGG TGTGGCACGA GACTTGAACG ATCTTCGACG CGTTCACCAG 
CTCTTGGTGT CATCTCTGAA CAAGCTTCAT AAAGGGACCA GCTCTCACCT GTACAATGAG 
AGTGCTTCTA CTCTAGAGAA GCTGGCAATA TTGAAAGCAT GGTCTGAGGT TTTTGTTGTT 
GCAATGGAAC AGGAAAAACA ACTAAGCAAT GTGCCACCAT CCCAAGAGGA ACCTCAAAAC 
GATGATGATG GCAGTTGTGG ACAAGAAAGT CTCTTAAAGC TTGTAACACC TGAACTTGTA 
TCTTTAAGCA AGCACTGGCT TGCTGCTTTA AAGGATCATG CATTACTCTC ACTTCCTGCA 
GAGTTTTCCA GTCAGTTACC CCATGACGGA GGAGCATTTT ACACCCCTGA TACTATGGAT 
TCTGCTCGAC AGTATTATCG AAAGGCCTGG CCACCCATCC TTCATGCTGC AGCTCTTTGG 
CTCTGCACAG GAGGGTTTGA AGGTCTTGAA CGAGAGTTTG AGCAAGATGC AAACAACAGC 
GGAACAATTT TAGGACCTCC TGGAAGCCCT ATGAACAAAG TTATTTCAAA TCCTAATTCT 
GAGAGATTTC ATCTGCTCTT TGGTATCTGT ATGGAGGCAC TGTGTAGTCC TCGGTCGGCA 
GAAAGCCTAG AATGTGTACT TGTCTGTTTA CAAGCATTAC AGCTGCTTTT GAAACATCCA 
TTGCCAAAAA GCATTCTTGG CCAGGATCCT CAGTTGTGCA TTGAACTGTG TAATGTTTTA 
CATAGAATTT TGCTTACCCG GGAAACTCCC CGATGCCAGA TGATTGTGAT GAAAATTGCC 
CATCTTTTGC TTGAAGCAAG ACATGATGAC ATTCGTTCTC ATAAAAAGAA GAAACTGAAA 
GAGCTTGCCC CTGCTAATCA AGAACTAGAA GATGTTGACA CAGCATTAGC TGCACTTGGA 
GAAGGTGGTG AAACTGGTGA CATAGTACCG GGACAGTCAG TAGTGTTTGC TCTACTCGAG 
GTGTGTCTGT GTTTGTTAGT CAGGCAGCTC CCTCATATCA GTCCTGCACT AGCTAACAAT 
ACAGGCACGT TTGGCATTCA GGCAGCCACA CGATCCAAAA AGTTGACAGA AGAATCTTCA 
AAGTTGGTTG CTGCTACTCT GAGTGTCATG GCTGAACTTC CAAACCTTTG TTCGCCCAAA 
GGTAGCACCA CTGTCCTCCC GACCATTCTC TACTTGACAA CAGGAGTACT AAAAGAAACA 
GCTGTTCAGA CTACTGAAGA CAGAGGATTA GTGGTAGCCT CGCCTCCAGT AAGCACCACA 
TTGCAAAGTC TGAAATCTCT CTGCACCTCA CCTTTCACAA GGAACAAACT GTGTGGTTCA 
GAGTGGCTCA CTTTGCTCCA GAGTGCTTTA GCTCGGATTT TGGATCTCAG TTGTGAAGAT 
AGTAAACCAG ATGAAATTTC CATGTTGTTG GCTGTGGCAG TTTTCATTCT CTCAGCTCCT 
GGAGAAGTTG TGTCAACACC AAATCTTCAG TTTCCATGTA TCAATCTTTT TAAACAAGCA 
CTGCAGCTCG ATGGAGACCT GGTACAGCTT AAATGTGTTC AGACACTCCG TTCCATGTTT 
CAACACCCTG ATCGAAGTAT AGCCACTCCA TACATCCATA CTCTTGCTCC ACGAGTCGTG 
GAAATACTTG TGCAGTTGAC TGCTGATTTG ACAACTCAGT CTACTGTTAC TGATCGCCAG 
TTTAGTCTAG CCACAGAATG TCTGACCTGT CTAGACACAC TGGTTACTCT GGCTCCTTCT 
GATAAAAAAA TTCATATGCT GAGTCTGTAC ATCCCCCTGG TGGTCAACCT TTTGATAGAG 
CCAACATTAC AACAGGGGGC GTCACACAAC AGGAAGAAGT TACACGACCA TGCTTTGCAA 
CAGCTGATCA AAGTGGGCCC TCAATATCCC CAGGACTTCC GCTCCATCAT GACCCAAGCT 
CCCCAGCTGA GAGGGCGGCT TGAAAATGCC ATAAGGAAGG GTTCGCTAAT TCACGTTGGA 
AGCCGAGAAT CTTCCACCGC ACCTTCGAGT CAAAAAATAA ATTCCTCCCA AGCTCCAGCA 
ATCAAATTAA AGATGGATTT TAGCAATTTC ACTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022250202.1
CDS	1..6219
Translation	MMELSHSLTLNEEALSQIAEAKRPVFVFEWLRFLDKVLGAAQKSDIKECQKKLVEQLLNQVHESPGPPTRRLIARCLATLFSVGDTFLLFEAVNRCTDILKNKDDSPSVLPTRLGAITCIGAMYEKLGRMMGRSHEEAVTVMLKSLKNAESQTRCETMVTMEKIIAGMGTAAASVHKDIFKAAKHCMTDRVMPVRCAAAQCLQEMLKHVPFVYTSELETVASLCFRSFEGSNYEVRCAVANLLGTLVATTQQASQPAVGKNRLATFEEVLALMSSGFLRGGIGFLKGGAGEMIKGSSNVSREIRVGVTHAYVVLVRQLGGLWLERNLNTLLVHLLELVSSPRAATSHVDAVYSRKCIAFILRSVLGKMLGEKAQAAACKDLVQIIAKYSNADAAAESSGSKEGLQEAALAQHVLVCALQELGCLISSLGTTASSVVNDSSSGIIEAVVSVLIHSSAARLAAAWCLRSIATAVSSQLTPLLDRCLEQLDTLKASPEAICGYSGALSALLGAACYTALGIPHSKGKIIFNVAEDLLRTASQNSRLSVQRTQAGWLMIGAVMTLGPPVVRGLLPRMLLLWKNSFPRSTKELESEKARGDAFTWQVTLEGRAGALAAMASFLVHCKELVSEEVVRRLLSPVEAALAMLTSLTNLVKSYGQHLKAAVAMVRLRLYEVLTLLPAQSYEGSYTSLLRLLVAEFTLTENPANTTTSLLRSLCHTDDSVILGSWLQETDHKAIEDQMEPNRRLDRDNLQPNSASGSGALEHDLTALYKSLPKGESIPGPLPLGVAVIDMSVILFGQTFPYVAVKHRLQMLGHFSQCIRHTKASRQEAVQINIFTAVLSALKMLAETKTPLGPDDVRQAAASLVLGALSHPNPILRCAAGEALGRMAQVVGDGRFVAEMAQHSFDKLKTARDAVSRTGHSLTLGCLHRYVGGMGSGQHLNTSVSILLALANDTNAPVVQVWALHAIALIADSGGPMFRGYVEPTLSLALKLLLNIPPSNVDVLQCIGKSLSALITTVGPELQGNTNSISTARSSFLVACAIMQDHGDSLVHAEAISCLQQLHMFAPRHVNLSTLVPTLCCVLYSSHLLLRRAAVACLRQLSQREAKEVCEHALSWARESGGTTTGYNSNISIPAIGETGLPGVLFAMLDVENDLRLVGHIKDTLTSMLQALARENLVQWLSLCKDVLTAADSSSSSISSLKEAERELGDNEADVDDEERFKASEEPVSHPFVSPRWPTRVFSAISLRRIIEACDGNRAHFDLAMAREQKLIRPKEDFLVLHLSDLIRMAFMAATSESDPLRLEGLRALQLIIDKFSKVPEPEFPGHVILEQYQAQVGAALRPAFSPETPSHVTAMACQVCSAWIGSGVARDLNDLRRVHQLLVSSLNKLHKGTSSHLYNESASTLEKLAILKAWSEVFVVAMEQEKQLSNVPPSQEEPQNDDDGSCGQESLLKLVTPELVSLSKHWLAALKDHALLSLPAEFSSQLPHDGGAFYTPDTMDSARQYYRKAWPPILHAAALWLCTGGFEGLEREFEQDANNSGTILGPPGSPMNKVISNPNSERFHLLFGICMEALCSPRSAESLECVLVCLQALQLLLKHPLPKSILGQDPQLCIELCNVLHRILLTRETPRCQMIVMKIAHLLLEARHDDIRSHKKKKLKELAPANQELEDVDTALAALGEGGETGDIVPGQSVVFALLEVCLCLLVRQLPHISPALANNTGTFGIQAATRSKKLTEESSKLVAATLSVMAELPNLCSPKGSTTVLPTILYLTTGVLKETAVQTTEDRGLVVASPPVSTTLQSLKSLCTSPFTRNKLCGSEWLTLLQSALARILDLSCEDSKPDEISMLLAVAVFILSAPGEVVSTPNLQFPCINLFKQALQLDGDLVQLKCVQTLRSMFQHPDRSIATPYIHTLAPRVVEILVQLTADLTTQSTVTDRQFSLATECLTCLDTLVTLAPSDKKIHMLSLYIPLVVNLLIEPTLQQGASHNRKKLHDHALQQLIKVGPQYPQDFRSIMTQAPQLRGRLENAIRKGSLIHVGSRESSTAPSSQKINSSQAPAIKLKMDFSNFTG

Target ORF information:

RefSeq Version	XM_022394494.1
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Definition	Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022394494.1

ORF Insert Sequence:

ATGATGGAAC TATCCCACAG TTTGACTCTA AATGAGGAGG CATTGAGTCA AATAGCTGAA 
GCAAAAAGGC CAGTCTTTGT TTTTGAATGG TTACGATTTT TAGATAAAGT TCTTGGAGCA 
GCTCAGAAGT CAGACATTAA GGAATGCCAG AAAAAACTAG TAGAACAGTT GTTGAATCAA 
GTCCACGAGT CACCAGGACC TCCTACCAGA AGACTAATCG CAAGATGTCT TGCAACACTC 
TTCAGTGTTG GAGATACATT TCTCCTCTTC GAAGCAGTCA ATAGATGTAC AGATATTTTG 
AAAAACAAAG ATGATTCCCC CAGCGTCTTA CCGACAAGAC TGGGGGCAAT TACCTGTATA 
GGAGCCATGT ATGAAAAGCT GGGAAGAATG ATGGGGAGAT CACACGAAGA AGCTGTCACA 
GTTATGCTGA AGTCTCTTAA GAATGCCGAG TCACAGACTC GCTGTGAAAC AATGGTAACT 
ATGGAGAAGA TTATTGCTGG TATGGGAACA GCTGCTGCAT CTGTTCATAA AGATATCTTT 
AAAGCTGCTA AGCACTGTAT GACTGACCGT GTTATGCCAG TAAGATGCGC AGCTGCCCAG 
TGTTTACAGG AGATGCTGAA ACATGTACCA TTTGTTTATA CATCTGAACT GGAGACTGTG 
GCTTCCCTGT GTTTTCGATC CTTTGAAGGG TCTAACTACG AAGTTCGATG TGCTGTTGCT 
AATCTGCTTG GAACACTTGT TGCTACCACT CAACAGGCTT CACAGCCTGC AGTGGGTAAA 
AATCGCCTCG CAACATTCGA AGAAGTGTTA GCTTTGATGT CCTCGGGATT TCTTCGTGGT 
GGTATTGGTT TCTTGAAAGG TGGTGCAGGA GAGATGATTA AAGGTAGTTC TAATGTCAGT 
AGAGAAATCA GGGTTGGAGT GACACATGCT TATGTTGTTT TGGTTCGACA ACTTGGAGGC 
TTGTGGTTGG AAAGGAACCT GAATACCCTC TTAGTCCACC TTCTGGAGCT TGTTTCTAGT 
CCTAGAGCAG CAACCTCACA TGTTGATGCC GTTTACAGCC GCAAATGCAT TGCCTTCATC 
TTGCGTTCAG TTTTGGGCAA GATGCTAGGA GAAAAGGCTC AGGCAGCAGC TTGCAAAGAC 
TTAGTACAGA TTATTGCGAA GTATTCAAAT GCAGATGCTG CAGCAGAGTC CTCTGGCAGT 
AAAGAAGGCT TACAAGAAGC AGCCCTTGCT CAACATGTTC TCGTGTGTGC TCTGCAAGAG 
CTTGGTTGTT TAATATCTAG TCTTGGAACC ACAGCCTCTT CGGTAGTGAA TGATTCTTCC 
TCAGGTATAA TTGAAGCTGT GGTATCAGTT TTAATTCACT CAAGTGCAGC ACGACTTGCA 
GCTGCGTGGT GCTTGAGGTC CATTGCAACA GCTGTATCAT CTCAGCTAAC TCCATTGCTA 
GACCGATGTC TAGAGCAACT AGATACCCTT AAAGCGTCAC CCGAGGCTAT TTGTGGATAT 
TCTGGAGCTT TGTCGGCACT TCTTGGAGCA GCATGTTATA CTGCATTAGG CATCCCTCAC 
AGTAAAGGAA AGATCATATT CAATGTAGCG GAGGATCTTC TTCGCACAGC ATCACAGAAT 
AGTAGACTGT CTGTCCAACG TACTCAAGCT GGTTGGTTAA TGATTGGAGC AGTCATGACA 
TTGGGTCCAC CTGTGGTTCG TGGACTTCTG CCAAGAATGT TGCTTCTTTG GAAGAACTCT 
TTTCCAAGGT CAACAAAAGA ACTAGAATCA GAAAAGGCTA GAGGAGATGC TTTCACATGG 
CAAGTAACCC TTGAAGGAAG AGCAGGAGCA TTAGCAGCTA TGGCAAGTTT TCTGGTACAC 
TGTAAAGAAC TTGTCTCTGA AGAGGTGGTG CGTAGATTGC TGTCACCAGT TGAAGCTGCT 
CTTGCCATGC TAACAAGCTT AACAAACCTG GTCAAGTCAT ATGGACAGCA CTTGAAGGCA 
GCTGTTGCGA TGGTGAGACT ACGTCTTTAT GAAGTGCTAA CACTTTTGCC TGCCCAATCA 
TATGAAGGAA GTTACACCAG TCTTTTAAGG CTTTTGGTGG CTGAGTTTAC CCTGACTGAA 
AACCCAGCTA ATACTACTAC ATCGTTGCTT CGAAGCCTTT GCCACACTGA TGATAGTGTG 
ATATTGGGGT CCTGGCTGCA AGAAACTGAT CACAAGGCTA TTGAGGACCA GATGGAGCCA 
AACAGAAGAT TGGATAGGGA TAATCTTCAA CCAAATAGTG CATCGGGATC AGGTGCATTG 
GAACACGATT TGACAGCTCT GTATAAGTCA CTGCCAAAGG GGGAGTCAAT TCCAGGTCCT 
TTACCATTAG GAGTTGCTGT TATTGACATG TCTGTCATCC TGTTTGGCCA GACATTTCCT 
TATGTAGCTG TTAAGCACAG ACTGCAGATG TTAGGTCACT TCTCACAATG TATTAGGCAC 
ACTAAGGCCT CAAGACAGGA AGCTGTTCAA ATTAACATTT TCACAGCTGT TCTAAGTGCT 
CTAAAGATGC TTGCAGAGAC AAAAACTCCA CTAGGACCTG ATGACGTGAG GCAAGCTGCT 
GCTAGTTTAG TGCTGGGAGC CTTGAGTCAC CCAAATCCTA TACTACGCTG TGCCGCAGGA 
GAGGCTTTAG GACGAATGGC ACAGGTTGTG GGTGATGGCC GGTTTGTTGC AGAGATGGCT 
CAGCACAGCT TTGACAAATT AAAAACGGCC AGAGATGCTG TATCACGTAC AGGCCACTCT 
CTAACACTAG GATGTTTACA CCGATACGTT GGGGGAATGG GATCAGGCCA ACATCTTAAC 
ACTAGTGTTA GTATTTTGTT GGCATTAGCC AATGATACCA ATGCTCCTGT TGTTCAGGTA 
TGGGCTCTTC ATGCTATAGC CCTCATAGCA GATTCTGGGG GACCAATGTT CCGTGGATAT 
GTGGAGCCTA CCCTCTCTCT AGCTTTAAAA TTACTACTGA ATATCCCTCC TTCCAATGTA 
GACGTATTGC AGTGTATAGG AAAGAGTCTG TCTGCTTTGA TTACTACTGT GGGACCAGAA 
TTACAAGGAA ATACGAACTC CATCAGCACT GCCCGTTCTT CCTTTTTGGT AGCCTGTGCC 
ATAATGCAAG ATCATGGTGA CTCCCTAGTC CATGCTGAAG CCATTTCTTG CCTTCAGCAG 
TTACACATGT TTGCTCCACG TCACGTTAAT CTATCAACCC TTGTACCTAC CCTTTGTTGT 
GTATTATACT CTTCCCATCT TCTGTTAAGA AGAGCAGCAG TAGCCTGCTT AAGACAGCTG 
TCCCAGAGAG AGGCTAAGGA AGTGTGTGAA CATGCTCTTT CCTGGGCTAG AGAATCGGGT 
GGCACCACAA CTGGCTACAA CTCTAATATC AGCATTCCTG CAATAGGAGA GACTGGGCTA 
CCTGGTGTAC TCTTTGCCAT GCTGGACGTA GAGAATGACC TGAGGCTAGT AGGTCACATC 
AAGGACACAC TTACCAGCAT GTTACAGGCA CTAGCGAGGG AGAACCTAGT TCAGTGGTTG 
AGTTTATGTA AAGATGTTCT TACTGCTGCT GATTCTAGTT CATCCAGCAT ATCATCTTTG 
AAAGAAGCTG AGAGAGAATT AGGAGATAAT GAAGCAGATG TAGATGACGA GGAGAGATTT 
AAAGCTTCAG AAGAGCCAGT ATCGCATCCT TTTGTCTCTC CTCGTTGGCC CACTAGAGTG 
TTTTCTGCCA TTTCTTTGAG GAGGATTATT GAAGCATGTG ATGGCAATCG AGCTCATTTT 
GATTTAGCCA TGGCAAGGGA ACAGAAATTG ATTAGACCAA AAGAAGATTT TTTGGTTCTT 
CATCTGTCTG ACCTGATTAG GATGGCATTT ATGGCAGCTA CATCAGAAAG TGACCCTTTA 
AGGTTGGAAG GATTAAGAGC ATTGCAGTTA ATTATTGACA AGTTTTCCAA AGTTCCTGAA 
CCAGAATTTC CAGGTCATGT AATCTTGGAA CAGTATCAAG CTCAGGTTGG GGCTGCTCTT 
CGACCAGCAT TCTCACCAGA AACACCTTCT CATGTTACTG CTATGGCTTG TCAGGTGTGT 
AGTGCCTGGA TTGGAAGTGG TGTGGCACGA GACTTGAACG ATCTTCGACG CGTTCACCAG 
CTCTTGGTGT CATCTCTGAA CAAGCTTCAT AAAGGGACCA GCTCTCACCT GTACAATGAG 
AGTGCTTCTA CTCTAGAGAA GCTGGCAATA TTGAAAGCAT GGTCTGAGGT TTTTGTTGTT 
GCAATGGAAC AGGAAAAACA ACTAAGCAAT GTGCCACCAT CCCAAGAGGA ACCTCAAAAC 
GATGATGATG GCAGTTGTGG ACAAGAAAGT CTCTTAAAGC TTGTAACACC TGAACTTGTA 
TCTTTAAGCA AGCACTGGCT TGCTGCTTTA AAGGATCATG CATTACTCTC ACTTCCTGCA 
GAGTTTTCCA GTCAGTTACC CCATGACGGA GGAGCATTTT ACACCCCTGA TACTATGGAT 
TCTGCTCGAC AGTATTATCG AAAGGCCTGG CCACCCATCC TTCATGCTGC AGCTCTTTGG 
CTCTGCACAG GAGGGTTTGA AGGTCTTGAA CGAGAGTTTG AGCAAGATGC AAACAACAGC 
GGAACAATTT TAGGACCTCC TGGAAGCCCT ATGAACAAAG TTATTTCAAA TCCTAATTCT 
GAGAGATTTC ATCTGCTCTT TGGTATCTGT ATGGAGGCAC TGTGTAGTCC TCGGTCGGCA 
GAAAGCCTAG AATGTGTACT TGTCTGTTTA CAAGCATTAC AGCTGCTTTT GAAACATCCA 
TTGCCAAAAA GCATTCTTGG CCAGGATCCT CAGTTGTGCA TTGAACTGTG TAATGTTTTA 
CATAGAATTT TGCTTACCCG GGAAACTCCC CGATGCCAGA TGATTGTGAT GAAAATTGCC 
CATCTTTTGC TTGAAGCAAG ACATGATGAC ATTCGTTCTC ATAAAAAGAA GAAACTGAAA 
GAGCTTGCCC CTGCTAATCA AGAACTAGAA GATGTTGACA CAGCATTAGC TGCACTTGGA 
GAAGGTGGTG AAACTGGTGA CATAGTACCG GGACAGTCAG TAGTGTTTGC TCTACTCGAG 
GTGTGTCTGT GTTTGTTAGT CAGGCAGCTC CCTCATATCA GTCCTGCACT AGCTAACAAT 
ACAGGCACGT TTGGCATTCA GGCAGCCACA CGATCCAAAA AGTTGACAGA AGAATCTTCA 
AAGTTGGTTG CTGCTACTCT GAGTGTCATG GCTGAACTTC CAAACCTTTG TTCGCCCAAA 
GGTAGCACCA CTGTCCTCCC GACCATTCTC TACTTGACAA CAGGAGTACT AAAAGAAACA 
GCTGTTCAGA CTACTGAAGA CAGAGGATTA GTGGTAGCCT CGCCTCCAGT AAGCACCACA 
TTGCAAAGTC TGAAATCTCT CTGCACCTCA CCTTTCACAA GGAACAAACT GTGTGGTTCA 
GAGTGGCTCA CTTTGCTCCA GAGTGCTTTA GCTCGGATTT TGGATCTCAG TTGTGAAGAT 
AGTAAACCAG ATGAAATTTC CATGTTGTTG GCTGTGGCAG TTTTCATTCT CTCAGCTCCT 
GGAGAAGTTG TGTCAACACC AAATCTTCAG TTTCCATGTA TCAATCTTTT TAAACAAGCA 
CTGCAGCTCG ATGGAGACCT GGTACAGCTT AAATGTGTTC AGACACTCCG TTCCATGTTT 
CAACACCCTG ATCGAAGTAT AGCCACTCCA TACATCCATA CTCTTGCTCC ACGAGTCGTG 
GAAATACTTG TGCAGTTGAC TGCTGATTTG ACAACTCAGT CTACTGTTAC TGATCGCCAG 
TTTAGTCTAG CCACAGAATG TCTGACCTGT CTAGACACAC TGGTTACTCT GGCTCCTTCT 
GATAAAAAAA TTCATATGCT GAGTCTGTAC ATCCCCCTGG TGGTCAACCT TTTGATAGAG 
CCAACATTAC AACAGGGGGC GTCACACAAC AGGAAGAAGT TACACGACCA TGCTTTGCAA 
CAGCTGATCA AAGTGGGCCC TCAATATCCC CAGGACTTCC GCTCCATCAT GACCCAAGCT 
CCCCAGCTGA GAGGGCGGCT TGAAAATGCC ATAAGGAAGG GTTCGCTAAT TCACGTTGGA 
AGCCGAGAAT CTTCCACCGC ACCTTCGAGT CAAAAAATAA ATTCCTCCCA AGCTCCAGCA 
ATCAAATTAA AGATGGATTT TAGCAATTTC ACTGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106466475 ( XM_022394495.1 ) cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab) -> XP_022250203.1 Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), transcript variant X3, mRNA.

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CloneID	OLi98327
Clone ID Related Accession (Same CDS sequence)	XM_022394495.1
Accession Version	XM_022394495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504022400000
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Product	HEAT repeat-containing protein 5B-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013667881.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Limulus polyphemus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAC TATCCCACAG TTTGACTCTA AATGAGGAGG CATTGAGTCA AATAGCTGAA 
GCAAAAAGGC CAGTCTTTGT TTTTGAATGG TTACGATTTT TAGATAAAGT TCTTGGAGCA 
GCTCAGAAGT CAGACATTAA GGAATGCCAG AAAAAACTAG TAGAACAGTT GTTGAATCAA 
GTCCACGAGT CACCAGGACC TCCTACCAGA AGACTAATCG CAAGATGTCT TGCAACACTC 
TTCAGTGTTG GAGATACATT TCTCCTCTTC GAAGCAGTCA ATAGATGTAC AGATATTTTG 
AAAAACAAAG ATGATTCCCC CAGCGTCTTA CCGACAAGAC TGGGGGCAAT TACCTGTATA 
GGAGCCATGT ATGAAAAGCT GGGAAGAATG ATGGGGAGAT CACACGAAGA AGCTGTCACA 
GTTATGCTGA AGTCTCTTAA GAATGCCGAG TCACAGACTC GCTGTGAAAC AATGGTAACT 
ATGGAGAAGA TTATTGCTGG TATGGGAACA GCTGCTGCAT CTGTTCATAA AGATATCTTT 
AAAGCTGCTA AGCACTGTAT GACTGACCGT GTTATGCCAG TAAGATGCGC AGCTGCCCAG 
TGTTTACAGG AGATGCTGAA ACATGTACCA TTTGTTTATA CATCTGAACT GGAGACTGTG 
GCTTCCCTGT GTTTTCGATC CTTTGAAGGG TCTAACTACG AAGTTCGATG TGCTGTTGCT 
AATCTGCTTG GAACACTTGT TGCTACCACT CAACAGGCTT CACAGCCTGC AGTGGGTAAA 
AATCGCCTCG CAACATTCGA AGAAGTGTTA GCTTTGATGT CCTCGGGATT TCTTCGTGGT 
GGTATTGGTT TCTTGAAAGG TGGTGCAGGA GAGATGATTA AAGGTAGTTC TAATGTCAGT 
AGAGAAATCA GGGTTGGAGT GACACATGCT TATGTTGTTT TGGTTCGACA ACTTGGAGGC 
TTGTGGTTGG AAAGGAACCT GAATACCCTC TTAGTCCACC TTCTGGAGCT TGTTTCTAGT 
CCTAGAGCAG CAACCTCACA TGTTGATGCC GTTTACAGCC GCAAATGCAT TGCCTTCATC 
TTGCGTTCAG TTTTGGGCAA GATGCTAGGA GAAAAGGCTC AGGCAGCAGC TTGCAAAGAC 
TTAGTACAGA TTATTGCGAA GTATTCAAAT GCAGATGCTG CAGCAGAGTC CTCTGGCAGT 
AAAGAAGGCT TACAAGAAGC AGCCCTTGCT CAACATGTTC TCGTGTGTGC TCTGCAAGAG 
CTTGGTTGTT TAATATCTAG TCTTGGAACC ACAGCCTCTT CGGTAGTGAA TGATTCTTCC 
TCAGGTATAA TTGAAGCTGT GGTATCAGTT TTAATTCACT CAAGTGCAGC ACGACTTGCA 
GCTGCGTGGT GCTTGAGGTC CATTGCAACA GCTGTATCAT CTCAGCTAAC TCCATTGCTA 
GACCGATGTC TAGAGCAACT AGATACCCTT AAAGCGTCAC CCGAGGCTAT TTGTGGATAT 
TCTGGAGCTT TGTCGGCACT TCTTGGAGCA GCATGTTATA CTGCATTAGG CATCCCTCAC 
AGTAAAGGAA AGATCATATT CAATGTAGCG GAGGATCTTC TTCGCACAGC ATCACAGAAT 
AGTAGACTGT CTGTCCAACG TACTCAAGCT GGTTGGTTAA TGATTGGAGC AGTCATGACA 
TTGGGTCCAC CTGTGGTTCG TGGACTTCTG CCAAGAATGT TGCTTCTTTG GAAGAACTCT 
TTTCCAAGGT CAACAAAAGA ACTAGAATCA GAAAAGGCTA GAGGAGATGC TTTCACATGG 
CAAGTAACCC TTGAAGGAAG AGCAGGAGCA TTAGCAGCTA TGGCAAGTTT TCTGGTACAC 
TGTAAAGAAC TTGTCTCTGA AGAGGTGGTG CGTAGATTGC TGTCACCAGT TGAAGCTGCT 
CTTGCCATGC TAACAAGCTT AACAAACCTG GTCAAGTCAT ATGGACAGCA CTTGAAGGCA 
GCTGTTGCGA TGGTGAGACT ACGTCTTTAT GAAGTGCTAA CACTTTTGCC TGCCCAATCA 
TATGAAGGAA GTTACACCAG TCTTTTAAGG CTTTTGGTGG CTGAGTTTAC CCTGACTGAA 
AACCCAGCTA ATACTACTAC ATCGTTGCTT CGAAGCCTTT GCCACACTGA TGATAGTGTG 
ATATTGGGGT CCTGGCTGCA AGAAACTGAT CACAAGGCTA TTGAGGACCA GCTTCAACCA 
AATAGTGCAT CGGGATCAGG TGCATTGGAA CACGATTTGA CAGCTCTGTA TAAGTCACTG 
CCAAAGGGGG AGTCAATTCC AGGTCCTTTA CCATTAGGAG TTGCTGTTAT TGACATGTCT 
GTCATCCTGT TTGGCCAGAC ATTTCCTTAT GTAGCTGTTA AGCACAGACT GCAGATGTTA 
GGTCACTTCT CACAATGTAT TAGGCACACT AAGGCCTCAA GACAGGAAGC TGTTCAAATT 
AACATTTTCA CAGCTGTTCT AAGTGCTCTA AAGATGCTTG CAGAGACAAA AACTCCACTA 
GGACCTGATG ACGTGAGGCA AGCTGCTGCT AGTTTAGTGC TGGGAGCCTT GAGTCACCCA 
AATCCTATAC TACGCTGTGC CGCAGGAGAG GCTTTAGGAC GAATGGCACA GGTTGTGGGT 
GATGGCCGGT TTGTTGCAGA GATGGCTCAG CACAGCTTTG ACAAATTAAA AACGGCCAGA 
GATGCTGTAT CACGTACAGG CCACTCTCTA ACACTAGGAT GTTTACACCG ATACGTTGGG 
GGAATGGGAT CAGGCCAACA TCTTAACACT AGTGTTAGTA TTTTGTTGGC ATTAGCCAAT 
GATACCAATG CTCCTGTTGT TCAGGTATGG GCTCTTCATG CTATAGCCCT CATAGCAGAT 
TCTGGGGGAC CAATGTTCCG TGGATATGTG GAGCCTACCC TCTCTCTAGC TTTAAAATTA 
CTACTGAATA TCCCTCCTTC CAATGTAGAC GTATTGCAGT GTATAGGAAA GAGTCTGTCT 
GCTTTGATTA CTACTGTGGG ACCAGAATTA CAAGGAAATA CGAACTCCAT CAGCACTGCC 
CGTTCTTCCT TTTTGGTAGC CTGTGCCATA ATGCAAGATC ATGGTGACTC CCTAGTCCAT 
GCTGAAGCCA TTTCTTGCCT TCAGCAGTTA CACATGTTTG CTCCACGTCA CGTTAATCTA 
TCAACCCTTG TACCTACCCT TTGTTGTGTA TTATACTCTT CCCATCTTCT GTTAAGAAGA 
GCAGCAGTAG CCTGCTTAAG ACAGCTGTCC CAGAGAGAGG CTAAGGAAGT GTGTGAACAT 
GCTCTTTCCT GGGCTAGAGA ATCGGGTGGC ACCACAACTG GCTACAACTC TAATATCAGC 
ATTCCTGCAA TAGGAGAGAC TGGGCTACCT GGTGTACTCT TTGCCATGCT GGACGTAGAG 
AATGACCTGA GGCTAGTAGG TCACATCAAG GACACACTTA CCAGCATGTT ACAGGCACTA 
GCGAGGGAGA ACCTAGTTCA GTGGTTGAGT TTATGTAAAG ATGTTCTTAC TGCTGCTGAT 
TCTAGTTCAT CCAGCATATC ATCTTTGAAA GAAGCTGAGA GAGAATTAGG AGATAATGAA 
GCAGATGTAG ATGACGAGGA GAGATTTAAA GCTTCAGAAG AGCCAGTATC GCATCCTTTT 
GTCTCTCCTC GTTGGCCCAC TAGAGTGTTT TCTGCCATTT CTTTGAGGAG GATTATTGAA 
GCATGTGATG GCAATCGAGC TCATTTTGAT TTAGCCATGG CAAGGGAACA GAAATTGATT 
AGACCAAAAG AAGATTTTTT GGTTCTTCAT CTGTCTGACC TGATTAGGAT GGCATTTATG 
GCAGCTACAT CAGAAAGTGA CCCTTTAAGG TTGGAAGGAT TAAGAGCATT GCAGTTAATT 
ATTGACAAGT TTTCCAAAGT TCCTGAACCA GAATTTCCAG GTCATGTAAT CTTGGAACAG 
TATCAAGCTC AGGTTGGGGC TGCTCTTCGA CCAGCATTCT CACCAGAAAC ACCTTCTCAT 
GTTACTGCTA TGGCTTGTCA GGTGTGTAGT GCCTGGATTG GAAGTGGTGT GGCACGAGAC 
TTGAACGATC TTCGACGCGT TCACCAGCTC TTGGTGTCAT CTCTGAACAA GCTTCATAAA 
GGGACCAGCT CTCACCTGTA CAATGAGAGT GCTTCTACTC TAGAGAAGCT GGCAATATTG 
AAAGCATGGT CTGAGGTTTT TGTTGTTGCA ATGGAACAGG AAAAACAACT AAGCAATGTG 
CCACCATCCC AAGAGGAACC TCAAAACGAT GATGATGGCA GTTGTGGACA AGAAAGTCTC 
TTAAAGCTTG TAACACCTGA ACTTGTATCT TTAAGCAAGC ACTGGCTTGC TGCTTTAAAG 
GATCATGCAT TACTCTCACT TCCTGCAGAG TTTTCCAGTC AGTTACCCCA TGACGGAGGA 
GCATTTTACA CCCCTGATAC TATGGATTCT GCTCGACAGT ATTATCGAAA GGCCTGGCCA 
CCCATCCTTC ATGCTGCAGC TCTTTGGCTC TGCACAGGAG GGTTTGAAGG TCTTGAACGA 
GAGTTTGAGC AAGATGCAAA CAACAGCGGA ACAATTTTAG GACCTCCTGG AAGCCCTATG 
AACAAAGTTA TTTCAAATCC TAATTCTGAG AGATTTCATC TGCTCTTTGG TATCTGTATG 
GAGGCACTGT GTAGTCCTCG GTCGGCAGAA AGCCTAGAAT GTGTACTTGT CTGTTTACAA 
GCATTACAGC TGCTTTTGAA ACATCCATTG CCAAAAAGCA TTCTTGGCCA GGATCCTCAG 
TTGTGCATTG AACTGTGTAA TGTTTTACAT AGAATTTTGC TTACCCGGGA AACTCCCCGA 
TGCCAGATGA TTGTGATGAA AATTGCCCAT CTTTTGCTTG AAGCAAGACA TGATGACATT 
CGTTCTCATA AAAAGAAGAA ACTGAAAGAG CTTGCCCCTG CTAATCAAGA ACTAGAAGAT 
GTTGACACAG CATTAGCTGC ACTTGGAGAA GGTGGTGAAA CTGGTGACAT AGTACCGGGA 
CAGTCAGTAG TGTTTGCTCT ACTCGAGGTG TGTCTGTGTT TGTTAGTCAG GCAGCTCCCT 
CATATCAGTC CTGCACTAGC TAACAATACA GGCACGTTTG GCATTCAGGC AGCCACACGA 
TCCAAAAAGT TGACAGAAGA ATCTTCAAAG TTGGTTGCTG CTACTCTGAG TGTCATGGCT 
GAACTTCCAA ACCTTTGTTC GCCCAAAGGT AGCACCACTG TCCTCCCGAC CATTCTCTAC 
TTGACAACAG GAGTACTAAA AGAAACAGCT GTTCAGACTA CTGAAGACAG AGGATTAGTG 
GTAGCCTCGC CTCCAGTAAG CACCACATTG CAAAGTCTGA AATCTCTCTG CACCTCACCT 
TTCACAAGGA ACAAACTGTG TGGTTCAGAG TGGCTCACTT TGCTCCAGAG TGCTTTAGCT 
CGGATTTTGG ATCTCAGTAA GACAAGTTGT GAAGATAGTA AACCAGATGA AATTTCCATG 
TTGTTGGCTG TGGCAGTTTT CATTCTCTCA GCTCCTGGAG AAGTTGTGTC AACACCAAAT 
CTTCAGTTTC CATGTATCAA TCTTTTTAAA CAAGCACTGC AGCTCGATGG AGACCTGGTA 
CAGCTTAAAT GTGTTCAGAC ACTCCGTTCC ATGTTTCAAC ACCCTGATCG AAGTATAGCC 
ACTCCATACA TCCATACTCT TGCTCCACGA GTCGTGGAAA TACTTGTGCA GTTGACTGCT 
GATTTGACAA CTCAGTCTAC TGTTACTGAT CGCCAGTTTA GTCTAGCCAC AGAATGTCTG 
ACCTGTCTAG ACACACTGGT TACTCTGGCT CCTTCTGATA AAAAAATTCA TATGCTGAGT 
CTGTACATCC CCCTGGTGGT CAACCTTTTG ATAGAGCCAA CATTACAACA GGGGGCGTCA 
CACAACAGGA AGAAGTTACA CGACCATGCT TTGCAACAGC TGATCAAAGT GGGCCCTCAA 
TATCCCCAGG ACTTCCGCTC CATCATGACC CAAGCTCCCC AGCTGAGAGG GCGGCTTGAA 
AATGCCATAA GGAAGGGTTC GCTAATTCAC GTTGGAAGCC GAGAATCTTC CACCGCACCT 
TCGAGTCAAA AAATAAATTC CTCCCAAGCT CCAGCAATCA AATTAAAGAT GGATTTTAGC 
AATTTCACTG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022250203.1
CDS	1..6195
Translation	MMELSHSLTLNEEALSQIAEAKRPVFVFEWLRFLDKVLGAAQKSDIKECQKKLVEQLLNQVHESPGPPTRRLIARCLATLFSVGDTFLLFEAVNRCTDILKNKDDSPSVLPTRLGAITCIGAMYEKLGRMMGRSHEEAVTVMLKSLKNAESQTRCETMVTMEKIIAGMGTAAASVHKDIFKAAKHCMTDRVMPVRCAAAQCLQEMLKHVPFVYTSELETVASLCFRSFEGSNYEVRCAVANLLGTLVATTQQASQPAVGKNRLATFEEVLALMSSGFLRGGIGFLKGGAGEMIKGSSNVSREIRVGVTHAYVVLVRQLGGLWLERNLNTLLVHLLELVSSPRAATSHVDAVYSRKCIAFILRSVLGKMLGEKAQAAACKDLVQIIAKYSNADAAAESSGSKEGLQEAALAQHVLVCALQELGCLISSLGTTASSVVNDSSSGIIEAVVSVLIHSSAARLAAAWCLRSIATAVSSQLTPLLDRCLEQLDTLKASPEAICGYSGALSALLGAACYTALGIPHSKGKIIFNVAEDLLRTASQNSRLSVQRTQAGWLMIGAVMTLGPPVVRGLLPRMLLLWKNSFPRSTKELESEKARGDAFTWQVTLEGRAGALAAMASFLVHCKELVSEEVVRRLLSPVEAALAMLTSLTNLVKSYGQHLKAAVAMVRLRLYEVLTLLPAQSYEGSYTSLLRLLVAEFTLTENPANTTTSLLRSLCHTDDSVILGSWLQETDHKAIEDQLQPNSASGSGALEHDLTALYKSLPKGESIPGPLPLGVAVIDMSVILFGQTFPYVAVKHRLQMLGHFSQCIRHTKASRQEAVQINIFTAVLSALKMLAETKTPLGPDDVRQAAASLVLGALSHPNPILRCAAGEALGRMAQVVGDGRFVAEMAQHSFDKLKTARDAVSRTGHSLTLGCLHRYVGGMGSGQHLNTSVSILLALANDTNAPVVQVWALHAIALIADSGGPMFRGYVEPTLSLALKLLLNIPPSNVDVLQCIGKSLSALITTVGPELQGNTNSISTARSSFLVACAIMQDHGDSLVHAEAISCLQQLHMFAPRHVNLSTLVPTLCCVLYSSHLLLRRAAVACLRQLSQREAKEVCEHALSWARESGGTTTGYNSNISIPAIGETGLPGVLFAMLDVENDLRLVGHIKDTLTSMLQALARENLVQWLSLCKDVLTAADSSSSSISSLKEAERELGDNEADVDDEERFKASEEPVSHPFVSPRWPTRVFSAISLRRIIEACDGNRAHFDLAMAREQKLIRPKEDFLVLHLSDLIRMAFMAATSESDPLRLEGLRALQLIIDKFSKVPEPEFPGHVILEQYQAQVGAALRPAFSPETPSHVTAMACQVCSAWIGSGVARDLNDLRRVHQLLVSSLNKLHKGTSSHLYNESASTLEKLAILKAWSEVFVVAMEQEKQLSNVPPSQEEPQNDDDGSCGQESLLKLVTPELVSLSKHWLAALKDHALLSLPAEFSSQLPHDGGAFYTPDTMDSARQYYRKAWPPILHAAALWLCTGGFEGLEREFEQDANNSGTILGPPGSPMNKVISNPNSERFHLLFGICMEALCSPRSAESLECVLVCLQALQLLLKHPLPKSILGQDPQLCIELCNVLHRILLTRETPRCQMIVMKIAHLLLEARHDDIRSHKKKKLKELAPANQELEDVDTALAALGEGGETGDIVPGQSVVFALLEVCLCLLVRQLPHISPALANNTGTFGIQAATRSKKLTEESSKLVAATLSVMAELPNLCSPKGSTTVLPTILYLTTGVLKETAVQTTEDRGLVVASPPVSTTLQSLKSLCTSPFTRNKLCGSEWLTLLQSALARILDLSKTSCEDSKPDEISMLLAVAVFILSAPGEVVSTPNLQFPCINLFKQALQLDGDLVQLKCVQTLRSMFQHPDRSIATPYIHTLAPRVVEILVQLTADLTTQSTVTDRQFSLATECLTCLDTLVTLAPSDKKIHMLSLYIPLVVNLLIEPTLQQGASHNRKKLHDHALQQLIKVGPQYPQDFRSIMTQAPQLRGRLENAIRKGSLIHVGSRESSTAPSSQKINSSQAPAIKLKMDFSNFTG

Target ORF information:

RefSeq Version	XM_022394495.1
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Definition	Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022394495.1

ORF Insert Sequence:

ATGATGGAAC TATCCCACAG TTTGACTCTA AATGAGGAGG CATTGAGTCA AATAGCTGAA 
GCAAAAAGGC CAGTCTTTGT TTTTGAATGG TTACGATTTT TAGATAAAGT TCTTGGAGCA 
GCTCAGAAGT CAGACATTAA GGAATGCCAG AAAAAACTAG TAGAACAGTT GTTGAATCAA 
GTCCACGAGT CACCAGGACC TCCTACCAGA AGACTAATCG CAAGATGTCT TGCAACACTC 
TTCAGTGTTG GAGATACATT TCTCCTCTTC GAAGCAGTCA ATAGATGTAC AGATATTTTG 
AAAAACAAAG ATGATTCCCC CAGCGTCTTA CCGACAAGAC TGGGGGCAAT TACCTGTATA 
GGAGCCATGT ATGAAAAGCT GGGAAGAATG ATGGGGAGAT CACACGAAGA AGCTGTCACA 
GTTATGCTGA AGTCTCTTAA GAATGCCGAG TCACAGACTC GCTGTGAAAC AATGGTAACT 
ATGGAGAAGA TTATTGCTGG TATGGGAACA GCTGCTGCAT CTGTTCATAA AGATATCTTT 
AAAGCTGCTA AGCACTGTAT GACTGACCGT GTTATGCCAG TAAGATGCGC AGCTGCCCAG 
TGTTTACAGG AGATGCTGAA ACATGTACCA TTTGTTTATA CATCTGAACT GGAGACTGTG 
GCTTCCCTGT GTTTTCGATC CTTTGAAGGG TCTAACTACG AAGTTCGATG TGCTGTTGCT 
AATCTGCTTG GAACACTTGT TGCTACCACT CAACAGGCTT CACAGCCTGC AGTGGGTAAA 
AATCGCCTCG CAACATTCGA AGAAGTGTTA GCTTTGATGT CCTCGGGATT TCTTCGTGGT 
GGTATTGGTT TCTTGAAAGG TGGTGCAGGA GAGATGATTA AAGGTAGTTC TAATGTCAGT 
AGAGAAATCA GGGTTGGAGT GACACATGCT TATGTTGTTT TGGTTCGACA ACTTGGAGGC 
TTGTGGTTGG AAAGGAACCT GAATACCCTC TTAGTCCACC TTCTGGAGCT TGTTTCTAGT 
CCTAGAGCAG CAACCTCACA TGTTGATGCC GTTTACAGCC GCAAATGCAT TGCCTTCATC 
TTGCGTTCAG TTTTGGGCAA GATGCTAGGA GAAAAGGCTC AGGCAGCAGC TTGCAAAGAC 
TTAGTACAGA TTATTGCGAA GTATTCAAAT GCAGATGCTG CAGCAGAGTC CTCTGGCAGT 
AAAGAAGGCT TACAAGAAGC AGCCCTTGCT CAACATGTTC TCGTGTGTGC TCTGCAAGAG 
CTTGGTTGTT TAATATCTAG TCTTGGAACC ACAGCCTCTT CGGTAGTGAA TGATTCTTCC 
TCAGGTATAA TTGAAGCTGT GGTATCAGTT TTAATTCACT CAAGTGCAGC ACGACTTGCA 
GCTGCGTGGT GCTTGAGGTC CATTGCAACA GCTGTATCAT CTCAGCTAAC TCCATTGCTA 
GACCGATGTC TAGAGCAACT AGATACCCTT AAAGCGTCAC CCGAGGCTAT TTGTGGATAT 
TCTGGAGCTT TGTCGGCACT TCTTGGAGCA GCATGTTATA CTGCATTAGG CATCCCTCAC 
AGTAAAGGAA AGATCATATT CAATGTAGCG GAGGATCTTC TTCGCACAGC ATCACAGAAT 
AGTAGACTGT CTGTCCAACG TACTCAAGCT GGTTGGTTAA TGATTGGAGC AGTCATGACA 
TTGGGTCCAC CTGTGGTTCG TGGACTTCTG CCAAGAATGT TGCTTCTTTG GAAGAACTCT 
TTTCCAAGGT CAACAAAAGA ACTAGAATCA GAAAAGGCTA GAGGAGATGC TTTCACATGG 
CAAGTAACCC TTGAAGGAAG AGCAGGAGCA TTAGCAGCTA TGGCAAGTTT TCTGGTACAC 
TGTAAAGAAC TTGTCTCTGA AGAGGTGGTG CGTAGATTGC TGTCACCAGT TGAAGCTGCT 
CTTGCCATGC TAACAAGCTT AACAAACCTG GTCAAGTCAT ATGGACAGCA CTTGAAGGCA 
GCTGTTGCGA TGGTGAGACT ACGTCTTTAT GAAGTGCTAA CACTTTTGCC TGCCCAATCA 
TATGAAGGAA GTTACACCAG TCTTTTAAGG CTTTTGGTGG CTGAGTTTAC CCTGACTGAA 
AACCCAGCTA ATACTACTAC ATCGTTGCTT CGAAGCCTTT GCCACACTGA TGATAGTGTG 
ATATTGGGGT CCTGGCTGCA AGAAACTGAT CACAAGGCTA TTGAGGACCA GCTTCAACCA 
AATAGTGCAT CGGGATCAGG TGCATTGGAA CACGATTTGA CAGCTCTGTA TAAGTCACTG 
CCAAAGGGGG AGTCAATTCC AGGTCCTTTA CCATTAGGAG TTGCTGTTAT TGACATGTCT 
GTCATCCTGT TTGGCCAGAC ATTTCCTTAT GTAGCTGTTA AGCACAGACT GCAGATGTTA 
GGTCACTTCT CACAATGTAT TAGGCACACT AAGGCCTCAA GACAGGAAGC TGTTCAAATT 
AACATTTTCA CAGCTGTTCT AAGTGCTCTA AAGATGCTTG CAGAGACAAA AACTCCACTA 
GGACCTGATG ACGTGAGGCA AGCTGCTGCT AGTTTAGTGC TGGGAGCCTT GAGTCACCCA 
AATCCTATAC TACGCTGTGC CGCAGGAGAG GCTTTAGGAC GAATGGCACA GGTTGTGGGT 
GATGGCCGGT TTGTTGCAGA GATGGCTCAG CACAGCTTTG ACAAATTAAA AACGGCCAGA 
GATGCTGTAT CACGTACAGG CCACTCTCTA ACACTAGGAT GTTTACACCG ATACGTTGGG 
GGAATGGGAT CAGGCCAACA TCTTAACACT AGTGTTAGTA TTTTGTTGGC ATTAGCCAAT 
GATACCAATG CTCCTGTTGT TCAGGTATGG GCTCTTCATG CTATAGCCCT CATAGCAGAT 
TCTGGGGGAC CAATGTTCCG TGGATATGTG GAGCCTACCC TCTCTCTAGC TTTAAAATTA 
CTACTGAATA TCCCTCCTTC CAATGTAGAC GTATTGCAGT GTATAGGAAA GAGTCTGTCT 
GCTTTGATTA CTACTGTGGG ACCAGAATTA CAAGGAAATA CGAACTCCAT CAGCACTGCC 
CGTTCTTCCT TTTTGGTAGC CTGTGCCATA ATGCAAGATC ATGGTGACTC CCTAGTCCAT 
GCTGAAGCCA TTTCTTGCCT TCAGCAGTTA CACATGTTTG CTCCACGTCA CGTTAATCTA 
TCAACCCTTG TACCTACCCT TTGTTGTGTA TTATACTCTT CCCATCTTCT GTTAAGAAGA 
GCAGCAGTAG CCTGCTTAAG ACAGCTGTCC CAGAGAGAGG CTAAGGAAGT GTGTGAACAT 
GCTCTTTCCT GGGCTAGAGA ATCGGGTGGC ACCACAACTG GCTACAACTC TAATATCAGC 
ATTCCTGCAA TAGGAGAGAC TGGGCTACCT GGTGTACTCT TTGCCATGCT GGACGTAGAG 
AATGACCTGA GGCTAGTAGG TCACATCAAG GACACACTTA CCAGCATGTT ACAGGCACTA 
GCGAGGGAGA ACCTAGTTCA GTGGTTGAGT TTATGTAAAG ATGTTCTTAC TGCTGCTGAT 
TCTAGTTCAT CCAGCATATC ATCTTTGAAA GAAGCTGAGA GAGAATTAGG AGATAATGAA 
GCAGATGTAG ATGACGAGGA GAGATTTAAA GCTTCAGAAG AGCCAGTATC GCATCCTTTT 
GTCTCTCCTC GTTGGCCCAC TAGAGTGTTT TCTGCCATTT CTTTGAGGAG GATTATTGAA 
GCATGTGATG GCAATCGAGC TCATTTTGAT TTAGCCATGG CAAGGGAACA GAAATTGATT 
AGACCAAAAG AAGATTTTTT GGTTCTTCAT CTGTCTGACC TGATTAGGAT GGCATTTATG 
GCAGCTACAT CAGAAAGTGA CCCTTTAAGG TTGGAAGGAT TAAGAGCATT GCAGTTAATT 
ATTGACAAGT TTTCCAAAGT TCCTGAACCA GAATTTCCAG GTCATGTAAT CTTGGAACAG 
TATCAAGCTC AGGTTGGGGC TGCTCTTCGA CCAGCATTCT CACCAGAAAC ACCTTCTCAT 
GTTACTGCTA TGGCTTGTCA GGTGTGTAGT GCCTGGATTG GAAGTGGTGT GGCACGAGAC 
TTGAACGATC TTCGACGCGT TCACCAGCTC TTGGTGTCAT CTCTGAACAA GCTTCATAAA 
GGGACCAGCT CTCACCTGTA CAATGAGAGT GCTTCTACTC TAGAGAAGCT GGCAATATTG 
AAAGCATGGT CTGAGGTTTT TGTTGTTGCA ATGGAACAGG AAAAACAACT AAGCAATGTG 
CCACCATCCC AAGAGGAACC TCAAAACGAT GATGATGGCA GTTGTGGACA AGAAAGTCTC 
TTAAAGCTTG TAACACCTGA ACTTGTATCT TTAAGCAAGC ACTGGCTTGC TGCTTTAAAG 
GATCATGCAT TACTCTCACT TCCTGCAGAG TTTTCCAGTC AGTTACCCCA TGACGGAGGA 
GCATTTTACA CCCCTGATAC TATGGATTCT GCTCGACAGT ATTATCGAAA GGCCTGGCCA 
CCCATCCTTC ATGCTGCAGC TCTTTGGCTC TGCACAGGAG GGTTTGAAGG TCTTGAACGA 
GAGTTTGAGC AAGATGCAAA CAACAGCGGA ACAATTTTAG GACCTCCTGG AAGCCCTATG 
AACAAAGTTA TTTCAAATCC TAATTCTGAG AGATTTCATC TGCTCTTTGG TATCTGTATG 
GAGGCACTGT GTAGTCCTCG GTCGGCAGAA AGCCTAGAAT GTGTACTTGT CTGTTTACAA 
GCATTACAGC TGCTTTTGAA ACATCCATTG CCAAAAAGCA TTCTTGGCCA GGATCCTCAG 
TTGTGCATTG AACTGTGTAA TGTTTTACAT AGAATTTTGC TTACCCGGGA AACTCCCCGA 
TGCCAGATGA TTGTGATGAA AATTGCCCAT CTTTTGCTTG AAGCAAGACA TGATGACATT 
CGTTCTCATA AAAAGAAGAA ACTGAAAGAG CTTGCCCCTG CTAATCAAGA ACTAGAAGAT 
GTTGACACAG CATTAGCTGC ACTTGGAGAA GGTGGTGAAA CTGGTGACAT AGTACCGGGA 
CAGTCAGTAG TGTTTGCTCT ACTCGAGGTG TGTCTGTGTT TGTTAGTCAG GCAGCTCCCT 
CATATCAGTC CTGCACTAGC TAACAATACA GGCACGTTTG GCATTCAGGC AGCCACACGA 
TCCAAAAAGT TGACAGAAGA ATCTTCAAAG TTGGTTGCTG CTACTCTGAG TGTCATGGCT 
GAACTTCCAA ACCTTTGTTC GCCCAAAGGT AGCACCACTG TCCTCCCGAC CATTCTCTAC 
TTGACAACAG GAGTACTAAA AGAAACAGCT GTTCAGACTA CTGAAGACAG AGGATTAGTG 
GTAGCCTCGC CTCCAGTAAG CACCACATTG CAAAGTCTGA AATCTCTCTG CACCTCACCT 
TTCACAAGGA ACAAACTGTG TGGTTCAGAG TGGCTCACTT TGCTCCAGAG TGCTTTAGCT 
CGGATTTTGG ATCTCAGTAA GACAAGTTGT GAAGATAGTA AACCAGATGA AATTTCCATG 
TTGTTGGCTG TGGCAGTTTT CATTCTCTCA GCTCCTGGAG AAGTTGTGTC AACACCAAAT 
CTTCAGTTTC CATGTATCAA TCTTTTTAAA CAAGCACTGC AGCTCGATGG AGACCTGGTA 
CAGCTTAAAT GTGTTCAGAC ACTCCGTTCC ATGTTTCAAC ACCCTGATCG AAGTATAGCC 
ACTCCATACA TCCATACTCT TGCTCCACGA GTCGTGGAAA TACTTGTGCA GTTGACTGCT 
GATTTGACAA CTCAGTCTAC TGTTACTGAT CGCCAGTTTA GTCTAGCCAC AGAATGTCTG 
ACCTGTCTAG ACACACTGGT TACTCTGGCT CCTTCTGATA AAAAAATTCA TATGCTGAGT 
CTGTACATCC CCCTGGTGGT CAACCTTTTG ATAGAGCCAA CATTACAACA GGGGGCGTCA 
CACAACAGGA AGAAGTTACA CGACCATGCT TTGCAACAGC TGATCAAAGT GGGCCCTCAA 
TATCCCCAGG ACTTCCGCTC CATCATGACC CAAGCTCCCC AGCTGAGAGG GCGGCTTGAA 
AATGCCATAA GGAAGGGTTC GCTAATTCAC GTTGGAAGCC GAGAATCTTC CACCGCACCT 
TCGAGTCAAA AAATAAATTC CTCCCAAGCT CCAGCAATCA AATTAAAGAT GGATTTTAGC 
AATTTCACTG GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106466475 ( XM_022394496.1 ) cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab) -> XP_022250204.1 Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), transcript variant X4, mRNA.

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CloneID	OLi98328
Clone ID Related Accession (Same CDS sequence)	XM_022394496.1
Accession Version	XM_022394496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1504022400000
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Product	HEAT repeat-containing protein 5B-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013667881.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Limulus polyphemus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAC TATCCCACAG TTTGACTCTA AATGAGGAGG CATTGAGTCA AATAGCTGAA 
GCAAAAAGGC CAGTCTTTGT TTTTGAATGG TTACGATTTT TAGATAAAGT TCTTGGAGCA 
GCTCAGAAGT CAGACATTAA GGAATGCCAG AAAAAACTAG TAGAACAGTT GTTGAATCAA 
GTCCACGAGT CACCAGGACC TCCTACCAGA AGACTAATCG CAAGATGTCT TGCAACACTC 
TTCAGTGTTG GAGATACATT TCTCCTCTTC GAAGCAGTCA ATAGATGTAC AGATATTTTG 
AAAAACAAAG ATGATTCCCC CAGCGTCTTA CCGACAAGAC TGGGGGCAAT TACCTGTATA 
GGAGCCATGT ATGAAAAGCT GGGAAGAATG ATGGGGAGAT CACACGAAGA AGCTGTCACA 
GTTATGCTGA AGTCTCTTAA GAATGCCGAG TCACAGACTC GCTGTGAAAC AATGGTAACT 
ATGGAGAAGA TTATTGCTGG TATGGGAACA GCTGCTGCAT CTGTTCATAA AGATATCTTT 
AAAGCTGCTA AGCACTGTAT GACTGACCGT GTTATGCCAG TAAGATGCGC AGCTGCCCAG 
TGTTTACAGG AGATGCTGAA ACATGTACCA TTTGTTTATA CATCTGAACT GGAGACTGTG 
GCTTCCCTGT GTTTTCGATC CTTTGAAGGG TCTAACTACG AAGTTCGATG TGCTGTTGCT 
AATCTGCTTG GAACACTTGT TGCTACCACT CAACAGGCTT CACAGCCTGC AGTGGGTAAA 
AATCGCCTCG CAACATTCGA AGAAGTGTTA GCTTTGATGT CCTCGGGATT TCTTCGTGGT 
GGTATTGGTT TCTTGAAAGG TGGTGCAGGA GAGATGATTA AAGGTAGTTC TAATGTCAGT 
AGAGAAATCA GGGTTGGAGT GACACATGCT TATGTTGTTT TGGTTCGACA ACTTGGAGGC 
TTGTGGTTGG AAAGGAACCT GAATACCCTC TTAGTCCACC TTCTGGAGCT TGTTTCTAGT 
CCTAGAGCAG CAACCTCACA TGTTGATGCC GTTTACAGCC GCAAATGCAT TGCCTTCATC 
TTGCGTTCAG TTTTGGGCAA GATGCTAGGA GAAAAGGCTC AGGCAGCAGC TTGCAAAGAC 
TTAGTACAGA TTATTGCGAA GTATTCAAAT GCAGATGCTG CAGCAGAGTC CTCTGGCAGT 
AAAGAAGGCT TACAAGAAGC AGCCCTTGCT CAACATGTTC TCGTGTGTGC TCTGCAAGAG 
CTTGGTTGTT TAATATCTAG TCTTGGAACC ACAGCCTCTT CGGTAGTGAA TGATTCTTCC 
TCAGGTATAA TTGAAGCTGT GGTATCAGTT TTAATTCACT CAAGTGCAGC ACGACTTGCA 
GCTGCGTGGT GCTTGAGGTC CATTGCAACA GCTGTATCAT CTCAGCTAAC TCCATTGCTA 
GACCGATGTC TAGAGCAACT AGATACCCTT AAAGCGTCAC CCGAGGCTAT TTGTGGATAT 
TCTGGAGCTT TGTCGGCACT TCTTGGAGCA GCATGTTATA CTGCATTAGG CATCCCTCAC 
AGTAAAGGAA AGATCATATT CAATGTAGCG GAGGATCTTC TTCGCACAGC ATCACAGAAT 
AGTAGACTGT CTGTCCAACG TACTCAAGCT GGTTGGTTAA TGATTGGAGC AGTCATGACA 
TTGGGTCCAC CTGTGGTTCG TGGACTTCTG CCAAGAATGT TGCTTCTTTG GAAGAACTCT 
TTTCCAAGGT CAACAAAAGA ACTAGAATCA GAAAAGGCTA GAGGAGATGC TTTCACATGG 
CAAGTAACCC TTGAAGGAAG AGCAGGAGCA TTAGCAGCTA TGGCAAGTTT TCTGGTACAC 
TGTAAAGAAC TTGTCTCTGA AGAGGTGGTG CGTAGATTGC TGTCACCAGT TGAAGCTGCT 
CTTGCCATGC TAACAAGCTT AACAAACCTG GTCAAGTCAT ATGGACAGCA CTTGAAGGCA 
GCTGTTGCGA TGGTGAGACT ACGTCTTTAT GAAGTGCTAA CACTTTTGCC TGCCCAATCA 
TATGAAGGAA GTTACACCAG TCTTTTAAGG CTTTTGGTGG CTGAGTTTAC CCTGACTGAA 
AACCCAGCTA ATACTACTAC ATCGTTGCTT CGAAGCCTTT GCCACACTGA TGATAGTGTG 
ATATTGGGGT CCTGGCTGCA AGAAACTGAT CACAAGGCTA TTGAGGACCA GATGGAGCCA 
AACAGAAGAT TGGATAGGGA TAATCTTCAA CCAAATAGTG CATCGGGATC AGGTGCATTG 
GAACACGATT TGACAGCTCT GTATAAGTCA CTGCCAAAGG GGGAGTCAAT TCCAGGTCCT 
TTACCATTAG GAGTTGCTGT TATTGACATG TCTGTCATCC TGTTTGGCCA GACATTTCCT 
TATGTAGCTG TTAAGCACAG ACTGCAGATG TTAGGTCACT TCTCACAATG TATTAGGCAC 
ACTAAGGCCT CAAGACAGGA AGCTGTTCAA ATTAACATTT TCACAGCTGT TCTAAGTGCT 
CTAAAGATGC TTGCAGAGAC AAAAACTCCA CTAGGACCTG ATGACGTGAG GCAAGCTGCT 
GCTAGTTTAG TGCTGGGAGC CTTGAGTCAC CCAAATCCTA TACTACGCTG TGCCGCAGGA 
GAGGCTTTAG GACGAATGGC ACAGGTTGTG GGTGATGGCC GGTTTGTTGC AGAGATGGCT 
CAGCACAGCT TTGACAAATT AAAAACGGCC AGAGATGCTG TATCACGTAC AGGCCACTCT 
CTAACACTAG GATGTTTACA CCGATACGTT GGGGGAATGG GATCAGGCCA ACATCTTAAC 
ACTAGTGTTA GTATTTTGTT GGCATTAGCC AATGATACCA ATGCTCCTGT TGTTCAGGTA 
TGGGCTCTTC ATGCTATAGC CCTCATAGCA GATTCTGGGG GACCAATGTT CCGTGGATAT 
GTGGAGCCTA CCCTCTCTCT AGCTTTAAAA TTACTACTGA ATATCCCTCC TTCCAATGTA 
GACGTATTGC AGTGTATAGG AAAGAGTCTG TCTGCTTTGA TTACTACTGT GGGACCAGAA 
TTACAAGGAA ATACGAACTC CATCAGCACT GCCCGTTCTT CCTTTTTGGT AGCCTGTGCC 
ATAATGCAAG ATCATGGTGA CTCCCTAGTC CATGCTGAAG CCATTTCTTG CCTTCAGCAG 
TTACACATGT TTGCTCCACG TCACGTTAAT CTATCAACCC TTGTACCTAC CCTTTGTTGT 
GTATTATACT CTTCCCATCT TCTGTTAAGA AGAGCAGCAG TAGCCTGCTT AAGACAGCTG 
TCCCAGAGAG AGGCTAAGGA AGTGTGTGAA CATGCTCTTT CCTGGGCTAG AGAATCGGGT 
GGCACCACAA CTGGCTACAA CTCTAATATC AGCATTCCTG CAATAGGAGA GACTGGGCTA 
CCTGGTGTAC TCTTTGCCAT GCTGGACGTA GAGAATGACC TGAGGCTAGT AGGTCACATC 
AAGGACACAC TTACCAGCAT GTTACAGGCA CTAGCGAGGG AGAACCTAGT TCAGTGGTTG 
AGTTTATGTA AAGATGTTCT TACTGCTGCT GATTCTAGTT CATCCAGCAT ATCATCTTTG 
AAAGAAGCTG AGAGAGAATT AGGAGATAAT GAAGCAGATG TAGATGACGA GGAGAGATTT 
AAAGCTTCAG AAGAGCCAGT ATCGCATCCT TTTGTCTCTC CTCGTTGGCC CACTAGAGTG 
TTTTCTGCCA TTTCTTTGAG GAGGATTATT GAAGCATGTG ATGGCAATCG AGCTCATTTT 
GATTTAGCCA TGGCAAGGGA ACAGAAATTG ATTAGACCAA AAGAAGATTT TTTGGTTCTT 
CATCTGTCTG ACCTGATTAG GATGGCATTT ATGGCAGCTA CATCAGAAAG TGACCCTTTA 
AGGTTGGAAG GATTAAGAGC ATTGCAGTTA ATTATTGACA AGTTTTCCAA AGTTCCTGAA 
CCAGAATTTC CAGGTCATGT AATCTTGGAA CAGTATCAAG CTCAGGTTGG GGCTGCTCTT 
CGACCAGCAT TCTCACCAGA AACACCTTCT CATGTTACTG CTATGGCTTG TCAGGTGTGT 
AGTGCCTGGA TTGGAAGTGG TGTGGCACGA GACTTGAACG ATCTTCGACG CGTTCACCAG 
CTCTTGGTGT CATCTCTGAA CAAGCTTCAT AAAGGGACCA GCTCTCACCT GTACAATGAG 
AGTGCTTCTA CTCTAGAGAA GCTGGCAATA TTGAAAGCAT GGTCTGAGGT TTTTGTTGTT 
GCAATGGAAC AGGAAAAACA ACTAAGCAAT GTGCCACCAT CCCAAGAGGA ACCTCAAAAC 
GATGATGATG GCAGTTGTGG ACAAGAAAGT CTCTTAAAGC TTGTAACACC TGAACTTGTA 
TCTTTAAGCA AGCACTGGCT TGCTGCTTTA AAGGATCATG CATTACTCTC ACTTCCTGCA 
GAGTTTTCCA GTCAGTTACC CCATGACGGA GGAGCATTTT ACACCCCTGA TACTATGGAT 
TCTGCTCGAC AGTATTATCG AAAGGCCTGG CCACCCATCC TTCATGCTGC AGCTCTTTGG 
CTCTGCACAG GAGGGTTTGA AGGTCTTGAA CGAGAGTTTG AGCAAGATGC AAACAACAGC 
GGAACAATTT TAGGACCTCC TGGAAGCCCT ATGAACAAAG TTATTTCAAA TCCTAATTCT 
GAGAGATTTC ATCTGCTCTT TGGTATCTGT ATGGAGGCAC TGTGTAGTCC TCGGTCGGCA 
GAAAGCCTAG AATGTGTACT TGTCTGTTTA CAAGCATTAC AGCTGCTTTT GAAACATCCA 
TTGCCAAAAA GCATTCTTGG CCAGGATCCT CAGTTGTGCA TTGAACTGTG TAATGTTTTA 
CATAGAATTT TGCTTACCCG GGAAACTCCC CGATGCCAGA TGATTGTGAT GAAAATTGCC 
CATCTTTTGC TTGAAGCAAG ACATGATGAC ATTCGTTCTC ATAAAAAGAA GAAACTGAAA 
GAGCTTGCCC CTGCTAATCA AGAACTAGAA GATGTTGACA CAGCATTAGC TGCACTTGGA 
GAAGGTGGTG AAACTGGTGA CATAGTACCG GGACAGTCAG TAGTGTTTGC TCTACTCGAG 
GTGTGTCTGT GTTTGTTAGT CAGGCAGCTC CCTCATATCA GTCCTGCACT AGCTAACAAT 
ACAGGCACGT TTGGCATTCA GGCAGCCACA CGATCCAAAA AGTTGACAGA AGAATCTTCA 
AAGTTGGTTG CTGCTACTCT GAGTGTCATG GCTGAACTTC CAAACCTTTG TTCGCCCAAA 
GGTAGCACCA CTGTCCTCCC GACCATTCTC TACTTGACAA CAGGAGTACT AAAAGAAACA 
GCTGTTCAGA CTACTGAAGA CAGAGGATTA GTGGTAGCCT CGCCTCCAGT AAGCACCACA 
TTGCAAAGTC TGAAATCTCT CTGCACCTCA CCTTTCACAA GGAACAAACT GTGTGGTTCA 
GAGTGGCTCA CTTTGCTCCA GAGTGCTTTA GCTCGGATTT TGGATCTCAG TAAGACAAGT 
TGTGAAGATA GTAAACCAGA TGAAATTTCC ATGTTGTTGG CTGTGGCAGT TTTCATTCTC 
TCAGCTCCTG GAGAAGTTGT GTCAACACCA AATCTTCAGT TTCCATGTAT CAATCTTTTT 
AAACAAGCAC TGCAGCTCGA TGGAGACCTG GTACAGCTTA AATGTGTTCA GACACTCCGT 
TCCATGTTTC AACACCCTGA TCGAAGTATA GCCACTCCAT ACATCCATAC TCTTGCTCCA 
CGAGTCGTGG AAATACTTGT GCAGTTGACT GCTGATTTGA CAACTCAGTC TACTGTTACT 
GATCGCCAGT TTAGTCTAGC CACAGAATGT CTGACCTGTC TAGACACACT GGTTACTCTG 
GCTCCTTCTG ATAAAAGTAA AAATTCATAT GCTGAGTCTG TACATCCCCC TGGTGGTCAA 
CCTTTTGATA GAGCCAACAT TACAACAGGG GGCGTCACAC AACAGGAAGA AGTTACACGA 
CCATGCTTTG CAACAGCTGA TCAAAGTGGG CCCTCAATAT CCCCAGGACT TCCGCTCCAT 
CATGACCCAA GCTCCCCAGC TGAGAGGGCG GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022250204.1
CDS	1..6096
Translation	MMELSHSLTLNEEALSQIAEAKRPVFVFEWLRFLDKVLGAAQKSDIKECQKKLVEQLLNQVHESPGPPTRRLIARCLATLFSVGDTFLLFEAVNRCTDILKNKDDSPSVLPTRLGAITCIGAMYEKLGRMMGRSHEEAVTVMLKSLKNAESQTRCETMVTMEKIIAGMGTAAASVHKDIFKAAKHCMTDRVMPVRCAAAQCLQEMLKHVPFVYTSELETVASLCFRSFEGSNYEVRCAVANLLGTLVATTQQASQPAVGKNRLATFEEVLALMSSGFLRGGIGFLKGGAGEMIKGSSNVSREIRVGVTHAYVVLVRQLGGLWLERNLNTLLVHLLELVSSPRAATSHVDAVYSRKCIAFILRSVLGKMLGEKAQAAACKDLVQIIAKYSNADAAAESSGSKEGLQEAALAQHVLVCALQELGCLISSLGTTASSVVNDSSSGIIEAVVSVLIHSSAARLAAAWCLRSIATAVSSQLTPLLDRCLEQLDTLKASPEAICGYSGALSALLGAACYTALGIPHSKGKIIFNVAEDLLRTASQNSRLSVQRTQAGWLMIGAVMTLGPPVVRGLLPRMLLLWKNSFPRSTKELESEKARGDAFTWQVTLEGRAGALAAMASFLVHCKELVSEEVVRRLLSPVEAALAMLTSLTNLVKSYGQHLKAAVAMVRLRLYEVLTLLPAQSYEGSYTSLLRLLVAEFTLTENPANTTTSLLRSLCHTDDSVILGSWLQETDHKAIEDQMEPNRRLDRDNLQPNSASGSGALEHDLTALYKSLPKGESIPGPLPLGVAVIDMSVILFGQTFPYVAVKHRLQMLGHFSQCIRHTKASRQEAVQINIFTAVLSALKMLAETKTPLGPDDVRQAAASLVLGALSHPNPILRCAAGEALGRMAQVVGDGRFVAEMAQHSFDKLKTARDAVSRTGHSLTLGCLHRYVGGMGSGQHLNTSVSILLALANDTNAPVVQVWALHAIALIADSGGPMFRGYVEPTLSLALKLLLNIPPSNVDVLQCIGKSLSALITTVGPELQGNTNSISTARSSFLVACAIMQDHGDSLVHAEAISCLQQLHMFAPRHVNLSTLVPTLCCVLYSSHLLLRRAAVACLRQLSQREAKEVCEHALSWARESGGTTTGYNSNISIPAIGETGLPGVLFAMLDVENDLRLVGHIKDTLTSMLQALARENLVQWLSLCKDVLTAADSSSSSISSLKEAERELGDNEADVDDEERFKASEEPVSHPFVSPRWPTRVFSAISLRRIIEACDGNRAHFDLAMAREQKLIRPKEDFLVLHLSDLIRMAFMAATSESDPLRLEGLRALQLIIDKFSKVPEPEFPGHVILEQYQAQVGAALRPAFSPETPSHVTAMACQVCSAWIGSGVARDLNDLRRVHQLLVSSLNKLHKGTSSHLYNESASTLEKLAILKAWSEVFVVAMEQEKQLSNVPPSQEEPQNDDDGSCGQESLLKLVTPELVSLSKHWLAALKDHALLSLPAEFSSQLPHDGGAFYTPDTMDSARQYYRKAWPPILHAAALWLCTGGFEGLEREFEQDANNSGTILGPPGSPMNKVISNPNSERFHLLFGICMEALCSPRSAESLECVLVCLQALQLLLKHPLPKSILGQDPQLCIELCNVLHRILLTRETPRCQMIVMKIAHLLLEARHDDIRSHKKKKLKELAPANQELEDVDTALAALGEGGETGDIVPGQSVVFALLEVCLCLLVRQLPHISPALANNTGTFGIQAATRSKKLTEESSKLVAATLSVMAELPNLCSPKGSTTVLPTILYLTTGVLKETAVQTTEDRGLVVASPPVSTTLQSLKSLCTSPFTRNKLCGSEWLTLLQSALARILDLSKTSCEDSKPDEISMLLAVAVFILSAPGEVVSTPNLQFPCINLFKQALQLDGDLVQLKCVQTLRSMFQHPDRSIATPYIHTLAPRVVEILVQLTADLTTQSTVTDRQFSLATECLTCLDTLVTLAPSDKSKNSYAESVHPPGGQPFDRANITTGGVTQQEEVTRPCFATADQSGPSISPGLPLHHDPSSPAERAA

Target ORF information:

RefSeq Version	XM_022394496.1
Organism	Limulus polyphemus(Atlantic horseshoe crab)
Definition	Limulus polyphemus HEAT repeat-containing protein 5B-like (LOC106466475), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022394496.1

ORF Insert Sequence:

ATGATGGAAC TATCCCACAG TTTGACTCTA AATGAGGAGG CATTGAGTCA AATAGCTGAA 
GCAAAAAGGC CAGTCTTTGT TTTTGAATGG TTACGATTTT TAGATAAAGT TCTTGGAGCA 
GCTCAGAAGT CAGACATTAA GGAATGCCAG AAAAAACTAG TAGAACAGTT GTTGAATCAA 
GTCCACGAGT CACCAGGACC TCCTACCAGA AGACTAATCG CAAGATGTCT TGCAACACTC 
TTCAGTGTTG GAGATACATT TCTCCTCTTC GAAGCAGTCA ATAGATGTAC AGATATTTTG 
AAAAACAAAG ATGATTCCCC CAGCGTCTTA CCGACAAGAC TGGGGGCAAT TACCTGTATA 
GGAGCCATGT ATGAAAAGCT GGGAAGAATG ATGGGGAGAT CACACGAAGA AGCTGTCACA 
GTTATGCTGA AGTCTCTTAA GAATGCCGAG TCACAGACTC GCTGTGAAAC AATGGTAACT 
ATGGAGAAGA TTATTGCTGG TATGGGAACA GCTGCTGCAT CTGTTCATAA AGATATCTTT 
AAAGCTGCTA AGCACTGTAT GACTGACCGT GTTATGCCAG TAAGATGCGC AGCTGCCCAG 
TGTTTACAGG AGATGCTGAA ACATGTACCA TTTGTTTATA CATCTGAACT GGAGACTGTG 
GCTTCCCTGT GTTTTCGATC CTTTGAAGGG TCTAACTACG AAGTTCGATG TGCTGTTGCT 
AATCTGCTTG GAACACTTGT TGCTACCACT CAACAGGCTT CACAGCCTGC AGTGGGTAAA 
AATCGCCTCG CAACATTCGA AGAAGTGTTA GCTTTGATGT CCTCGGGATT TCTTCGTGGT 
GGTATTGGTT TCTTGAAAGG TGGTGCAGGA GAGATGATTA AAGGTAGTTC TAATGTCAGT 
AGAGAAATCA GGGTTGGAGT GACACATGCT TATGTTGTTT TGGTTCGACA ACTTGGAGGC 
TTGTGGTTGG AAAGGAACCT GAATACCCTC TTAGTCCACC TTCTGGAGCT TGTTTCTAGT 
CCTAGAGCAG CAACCTCACA TGTTGATGCC GTTTACAGCC GCAAATGCAT TGCCTTCATC 
TTGCGTTCAG TTTTGGGCAA GATGCTAGGA GAAAAGGCTC AGGCAGCAGC TTGCAAAGAC 
TTAGTACAGA TTATTGCGAA GTATTCAAAT GCAGATGCTG CAGCAGAGTC CTCTGGCAGT 
AAAGAAGGCT TACAAGAAGC AGCCCTTGCT CAACATGTTC TCGTGTGTGC TCTGCAAGAG 
CTTGGTTGTT TAATATCTAG TCTTGGAACC ACAGCCTCTT CGGTAGTGAA TGATTCTTCC 
TCAGGTATAA TTGAAGCTGT GGTATCAGTT TTAATTCACT CAAGTGCAGC ACGACTTGCA 
GCTGCGTGGT GCTTGAGGTC CATTGCAACA GCTGTATCAT CTCAGCTAAC TCCATTGCTA 
GACCGATGTC TAGAGCAACT AGATACCCTT AAAGCGTCAC CCGAGGCTAT TTGTGGATAT 
TCTGGAGCTT TGTCGGCACT TCTTGGAGCA GCATGTTATA CTGCATTAGG CATCCCTCAC 
AGTAAAGGAA AGATCATATT CAATGTAGCG GAGGATCTTC TTCGCACAGC ATCACAGAAT 
AGTAGACTGT CTGTCCAACG TACTCAAGCT GGTTGGTTAA TGATTGGAGC AGTCATGACA 
TTGGGTCCAC CTGTGGTTCG TGGACTTCTG CCAAGAATGT TGCTTCTTTG GAAGAACTCT 
TTTCCAAGGT CAACAAAAGA ACTAGAATCA GAAAAGGCTA GAGGAGATGC TTTCACATGG 
CAAGTAACCC TTGAAGGAAG AGCAGGAGCA TTAGCAGCTA TGGCAAGTTT TCTGGTACAC 
TGTAAAGAAC TTGTCTCTGA AGAGGTGGTG CGTAGATTGC TGTCACCAGT TGAAGCTGCT 
CTTGCCATGC TAACAAGCTT AACAAACCTG GTCAAGTCAT ATGGACAGCA CTTGAAGGCA 
GCTGTTGCGA TGGTGAGACT ACGTCTTTAT GAAGTGCTAA CACTTTTGCC TGCCCAATCA 
TATGAAGGAA GTTACACCAG TCTTTTAAGG CTTTTGGTGG CTGAGTTTAC CCTGACTGAA 
AACCCAGCTA ATACTACTAC ATCGTTGCTT CGAAGCCTTT GCCACACTGA TGATAGTGTG 
ATATTGGGGT CCTGGCTGCA AGAAACTGAT CACAAGGCTA TTGAGGACCA GATGGAGCCA 
AACAGAAGAT TGGATAGGGA TAATCTTCAA CCAAATAGTG CATCGGGATC AGGTGCATTG 
GAACACGATT TGACAGCTCT GTATAAGTCA CTGCCAAAGG GGGAGTCAAT TCCAGGTCCT 
TTACCATTAG GAGTTGCTGT TATTGACATG TCTGTCATCC TGTTTGGCCA GACATTTCCT 
TATGTAGCTG TTAAGCACAG ACTGCAGATG TTAGGTCACT TCTCACAATG TATTAGGCAC 
ACTAAGGCCT CAAGACAGGA AGCTGTTCAA ATTAACATTT TCACAGCTGT TCTAAGTGCT 
CTAAAGATGC TTGCAGAGAC AAAAACTCCA CTAGGACCTG ATGACGTGAG GCAAGCTGCT 
GCTAGTTTAG TGCTGGGAGC CTTGAGTCAC CCAAATCCTA TACTACGCTG TGCCGCAGGA 
GAGGCTTTAG GACGAATGGC ACAGGTTGTG GGTGATGGCC GGTTTGTTGC AGAGATGGCT 
CAGCACAGCT TTGACAAATT AAAAACGGCC AGAGATGCTG TATCACGTAC AGGCCACTCT 
CTAACACTAG GATGTTTACA CCGATACGTT GGGGGAATGG GATCAGGCCA ACATCTTAAC 
ACTAGTGTTA GTATTTTGTT GGCATTAGCC AATGATACCA ATGCTCCTGT TGTTCAGGTA 
TGGGCTCTTC ATGCTATAGC CCTCATAGCA GATTCTGGGG GACCAATGTT CCGTGGATAT 
GTGGAGCCTA CCCTCTCTCT AGCTTTAAAA TTACTACTGA ATATCCCTCC TTCCAATGTA 
GACGTATTGC AGTGTATAGG AAAGAGTCTG TCTGCTTTGA TTACTACTGT GGGACCAGAA 
TTACAAGGAA ATACGAACTC CATCAGCACT GCCCGTTCTT CCTTTTTGGT AGCCTGTGCC 
ATAATGCAAG ATCATGGTGA CTCCCTAGTC CATGCTGAAG CCATTTCTTG CCTTCAGCAG 
TTACACATGT TTGCTCCACG TCACGTTAAT CTATCAACCC TTGTACCTAC CCTTTGTTGT 
GTATTATACT CTTCCCATCT TCTGTTAAGA AGAGCAGCAG TAGCCTGCTT AAGACAGCTG 
TCCCAGAGAG AGGCTAAGGA AGTGTGTGAA CATGCTCTTT CCTGGGCTAG AGAATCGGGT 
GGCACCACAA CTGGCTACAA CTCTAATATC AGCATTCCTG CAATAGGAGA GACTGGGCTA 
CCTGGTGTAC TCTTTGCCAT GCTGGACGTA GAGAATGACC TGAGGCTAGT AGGTCACATC 
AAGGACACAC TTACCAGCAT GTTACAGGCA CTAGCGAGGG AGAACCTAGT TCAGTGGTTG 
AGTTTATGTA AAGATGTTCT TACTGCTGCT GATTCTAGTT CATCCAGCAT ATCATCTTTG 
AAAGAAGCTG AGAGAGAATT AGGAGATAAT GAAGCAGATG TAGATGACGA GGAGAGATTT 
AAAGCTTCAG AAGAGCCAGT ATCGCATCCT TTTGTCTCTC CTCGTTGGCC CACTAGAGTG 
TTTTCTGCCA TTTCTTTGAG GAGGATTATT GAAGCATGTG ATGGCAATCG AGCTCATTTT 
GATTTAGCCA TGGCAAGGGA ACAGAAATTG ATTAGACCAA AAGAAGATTT TTTGGTTCTT 
CATCTGTCTG ACCTGATTAG GATGGCATTT ATGGCAGCTA CATCAGAAAG TGACCCTTTA 
AGGTTGGAAG GATTAAGAGC ATTGCAGTTA ATTATTGACA AGTTTTCCAA AGTTCCTGAA 
CCAGAATTTC CAGGTCATGT AATCTTGGAA CAGTATCAAG CTCAGGTTGG GGCTGCTCTT 
CGACCAGCAT TCTCACCAGA AACACCTTCT CATGTTACTG CTATGGCTTG TCAGGTGTGT 
AGTGCCTGGA TTGGAAGTGG TGTGGCACGA GACTTGAACG ATCTTCGACG CGTTCACCAG 
CTCTTGGTGT CATCTCTGAA CAAGCTTCAT AAAGGGACCA GCTCTCACCT GTACAATGAG 
AGTGCTTCTA CTCTAGAGAA GCTGGCAATA TTGAAAGCAT GGTCTGAGGT TTTTGTTGTT 
GCAATGGAAC AGGAAAAACA ACTAAGCAAT GTGCCACCAT CCCAAGAGGA ACCTCAAAAC 
GATGATGATG GCAGTTGTGG ACAAGAAAGT CTCTTAAAGC TTGTAACACC TGAACTTGTA 
TCTTTAAGCA AGCACTGGCT TGCTGCTTTA AAGGATCATG CATTACTCTC ACTTCCTGCA 
GAGTTTTCCA GTCAGTTACC CCATGACGGA GGAGCATTTT ACACCCCTGA TACTATGGAT 
TCTGCTCGAC AGTATTATCG AAAGGCCTGG CCACCCATCC TTCATGCTGC AGCTCTTTGG 
CTCTGCACAG GAGGGTTTGA AGGTCTTGAA CGAGAGTTTG AGCAAGATGC AAACAACAGC 
GGAACAATTT TAGGACCTCC TGGAAGCCCT ATGAACAAAG TTATTTCAAA TCCTAATTCT 
GAGAGATTTC ATCTGCTCTT TGGTATCTGT ATGGAGGCAC TGTGTAGTCC TCGGTCGGCA 
GAAAGCCTAG AATGTGTACT TGTCTGTTTA CAAGCATTAC AGCTGCTTTT GAAACATCCA 
TTGCCAAAAA GCATTCTTGG CCAGGATCCT CAGTTGTGCA TTGAACTGTG TAATGTTTTA 
CATAGAATTT TGCTTACCCG GGAAACTCCC CGATGCCAGA TGATTGTGAT GAAAATTGCC 
CATCTTTTGC TTGAAGCAAG ACATGATGAC ATTCGTTCTC ATAAAAAGAA GAAACTGAAA 
GAGCTTGCCC CTGCTAATCA AGAACTAGAA GATGTTGACA CAGCATTAGC TGCACTTGGA 
GAAGGTGGTG AAACTGGTGA CATAGTACCG GGACAGTCAG TAGTGTTTGC TCTACTCGAG 
GTGTGTCTGT GTTTGTTAGT CAGGCAGCTC CCTCATATCA GTCCTGCACT AGCTAACAAT 
ACAGGCACGT TTGGCATTCA GGCAGCCACA CGATCCAAAA AGTTGACAGA AGAATCTTCA 
AAGTTGGTTG CTGCTACTCT GAGTGTCATG GCTGAACTTC CAAACCTTTG TTCGCCCAAA 
GGTAGCACCA CTGTCCTCCC GACCATTCTC TACTTGACAA CAGGAGTACT AAAAGAAACA 
GCTGTTCAGA CTACTGAAGA CAGAGGATTA GTGGTAGCCT CGCCTCCAGT AAGCACCACA 
TTGCAAAGTC TGAAATCTCT CTGCACCTCA CCTTTCACAA GGAACAAACT GTGTGGTTCA 
GAGTGGCTCA CTTTGCTCCA GAGTGCTTTA GCTCGGATTT TGGATCTCAG TAAGACAAGT 
TGTGAAGATA GTAAACCAGA TGAAATTTCC ATGTTGTTGG CTGTGGCAGT TTTCATTCTC 
TCAGCTCCTG GAGAAGTTGT GTCAACACCA AATCTTCAGT TTCCATGTAT CAATCTTTTT 
AAACAAGCAC TGCAGCTCGA TGGAGACCTG GTACAGCTTA AATGTGTTCA GACACTCCGT 
TCCATGTTTC AACACCCTGA TCGAAGTATA GCCACTCCAT ACATCCATAC TCTTGCTCCA 
CGAGTCGTGG AAATACTTGT GCAGTTGACT GCTGATTTGA CAACTCAGTC TACTGTTACT 
GATCGCCAGT TTAGTCTAGC CACAGAATGT CTGACCTGTC TAGACACACT GGTTACTCTG 
GCTCCTTCTG ATAAAAGTAA AAATTCATAT GCTGAGTCTG TACATCCCCC TGGTGGTCAA 
CCTTTTGATA GAGCCAACAT TACAACAGGG GGCGTCACAC AACAGGAAGA AGTTACACGA 
CCATGCTTTG CAACAGCTGA TCAAAGTGGG CCCTCAATAT CCCCAGGACT TCCGCTCCAT 
CATGACCCAA GCTCCCCAGC TGAGAGGGCG GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC106466475 cDNA ORF clone, Limulus polyphemus(Atlantic horseshoe crab)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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