REH2 cDNA ORF clone, Leishmania major strain Friedlin

Gene
Clones
gRNAs

Summary

Gene Symbol	REH2
Entrez Gene ID	5655100
Full Name	RNA editing associated helicase 2,putative;with=GeneDB:Tb927.4.1500
Gene Type	protein-coding
Organism	Leishmania major strain Friedlin

mRNA and Protein(s)

mRNA	Protein	Name
XM_001686375.1	XP_001686427.1	RNA editing associated helicase 2,putative;with=GeneDB:Tb927.4.1500

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

The genome of the kinetoplastid parasite, Leishmania major.
Ivens AC, Peacock CS, Worthey EA, Murphy L, Aggarwal G, Berriman M, Sisk E, Rajandream MA, Adlem E, Aert R, Anupama A, Apostolou Z, Attipoe P, Bason N, Bauser C, Beck A, Beverley SM, Bianchettin G, Borzym K, Bothe G, Bruschi CV, Collins M, Cadag E, Ciarloni L, Clayton C, Coulson RM, Cronin A, Cruz AK, Davies RM, De Gaudenzi J, Dobson DE, Duesterhoeft A, Fazelina G, Fosker N, Frasch AC, Fraser A, Fuchs M, Gabel C, Goble A, Goffeau A, Harris D, Hertz-Fowler C, Hilbert H, Horn D, Huang Y, Klages S, Knights A, Kube M, Larke N, Litvin L, Lord A, Louie T, Marra M, Masuy D, Matthews K, Michaeli S, Mottram JC, Müller-Auer S, Munden H, Nelson S, Norbertczak H, Oliver K, O'neil S, Pentony M, Pohl TM, Price C, Purnelle B, Quail MA, Rabbinowitsch E, Reinhardt R, Rieger M, Rinta J, Robben J, Robertson L, Ruiz JC, Rutter S, Saunders D, Schäfer M, Schein J, Schwartz DC, Seeger K, Seyler A, Sharp S, Shin H, Sivam D, Squares R, Squares S, Tosato V, Vogt C, Volckaert G, Wambutt R, Warren T, Wedler H, Woodward J, Zhou S, Zimmermann W, Smith DF, Blackwell JM, Stuart KD, Barrell B, Myler PJ
Science (New York, N.Y.)309(5733)436-42(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following REH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the REH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLb05456	XM_001686375.1 Latest version!	Leishmania major strain Friedlin RNA editing associated helicase 2,putative;with=GeneDB:Tb927.4.1500 (REH2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

REH2 ( XM_001686375.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_001686427.1 Leishmania major strain Friedlin RNA editing associated helicase 2,putative;with=GeneDB:Tb927.4.1500 (REH2), partial mRNA.

Price & Availability↑

CloneID	OLb05456
Clone ID Related Accession (Same CDS sequence)	XM_001686375.1
Accession Version	XM_001686375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1497456000000
Organism	Leishmania major strain Friedlin
Product	RNA editing associated helicase 2,putative;with=GeneDB:Tb927.4.1500
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007286). COMPLETENESS: incomplete on both ends.

ATGAGTGCGG CTGGCGGCTC GGGCACACCC AGGGCACTGT GGGCGCACGG AGTCGCCAAC 
ACGGCGAAAG AGGCTGAGCT GCTAGCCGCC ATGCACGCGG AGCAGATGGC GGACTTGTTA 
GGGTATCACG TCTTCTCCCT CCCATCAAGG CAGCACAAGC ACGCCGAGGC AGCACGCGCG 
GCAGGTCGCT ACGCGTCCTC GCCGGAGGAT AAGGACAGTG GCCCTTCGAC AGAGCAGCGA 
CTGGCGCAGT GGCCCCTGCC CTTGAGGCGC GTTCTGACCG ACGAAGAAAC GGAAGGCGGG 
GAATGGCAGC TTGTGAACAC GGCGACCTCC CTGCGACACT ACATCTCTAA GGAGCACACG 
TTGCTGTCGC CGTGCCTGCT GGACCCCAAG TCGCGACTCA GGATCGAGGC CCTGTTCGCG 
CGTGCACCTC ACGGCTGCCC CTCCTTTGCG CAACAGCTCA GGACTGAAAG GGTGCGTACG 
CTGACGGCGA GCAGCGCGGC GGGCGGCGGC GAAGAGGCGA TCGTGGCCCG GCTCCTCTTG 
CCATCACAGG TGACGGGTAT CACGAACTCC TCGATAGATC TCGTCGCCTC TGGCAAGGCG 
ATGGAGCGGT CAACGGCGGT GCTGCTGGCC TGCATGCACG CAGAGCTCCT GCTGGATGCC 
GTGGGCGTTG CTATTTTCGA CGACGCGGTG ACGCAGAAAG CGCACGCCAT GGCGGCCTGG 
AGCCACGGAC GCCCCGCACC ACTACCGGGC GCCAAGCCAA AAAATCCGTC GCACGTGCAC 
CTGCCACAGC CGCTGAAGGA GCTCGTCGTG GCGGCAGCAG GGGATGGTCG GTCTCGCACG 
TCGTCTCGCT CGGCTGAAGA GGACTGGTTT CACAGGCATA CCATTGTCAC TGAGCAGAGT 
GGGACCTTTG TAGAAACCGT TGTGGGCGAG TCCACCGCGA TGGAGGACAT CACCGCGTTT 
CTCCACACTC ACGGTGCCAT GCGGGCCGCC GCACCGTTTC TGCAGGTGGC AATCGGTTCC 
CGGGTCAAGT CCACAGTACT GCTGCCCTTG CCGGACCTCT ACGGCATTCG CGGTGGCGTG 
GGGATCGCGT CGAACGCGCA GGATGCTGAC ACCCTCGCTG CCATGCATGC ACTGGATGTG 
CTGTGCATGC TCGGTGTCCC GGTAAAGGCT ACGGCGGCGA TGGACGCCGC GTGGAAGCGC 
GTTCGCCGCG AGCGCATTCC CGACGTGCCC TGCGAGTCGG CGGACGTCTC CACGCCGAGC 
CCCCCGGCGC GCCGCTGCAC TGCCAAGAAG TTAGCCACGC AAGTGGCTAT AGCCGCAGCG 
GCCTTATCTG AAGATTTCGT GTCCGTGGAC GCAGCGCAGG CAAGCTCCTG CAACCACGCC 
AGCACATCCA CGCTGCCGCG GCGCCGTGCG GTGAAGCGAG CGCGACTGGC CTCGGATGCA 
TCCGCACCGG ATGATAGCAT ACCACAGGCA AACGCAACCG ACACGACTGC TGCCTCTCCG 
CCAAACGTGC CCGTGGTTGC GACATTGAGA GAGACTGGCG AGAAGCGGCT GGCAGCGGTG 
CGGGCGGTGG TGCCAAAGGA GTTCTGGGAT ATGCAGGCAG ACAGCCCTGA CGGGTACATT 
ATGATTGCCC CTGGCGTGCC GGAGGGATCT GCGGTGGCTG CGTATGCCAT TACGTCCCCG 
CGCAAGATGG ACAAATCGGC CAAGGGACGC GTGTTGGACT ACTTGGCGAC CGTGGGCCGT 
CGCCTCGACG ATGTCACCGT GGTGCGGCAA GCCCTCAGCG AGGAGGAGAC GGAGGGCCCA 
CCGCGGCACC GGTGTGCGCT GAAAGTGCCA GTGCCAGCCG TTTGCGGGGA AGCTCGCCTC 
GCGCTAGGCG AGGCCGACAC AGTCGCCGAC GCCGAAAACG CCGCCTGTAT GCACGCGGAG 
CTCATCCTGG ACACACTCGG GGTCTGCCTC TACACGGACT CTGTGAAGCA GAAGCGGCAC 
GCCGCGGCAT GCGCGCAGTG GGGTCGCTGG GCGCCTTCGA CTCCTGGCGA AGAACAGCCG 
AGCAGCACAC CGTCCCCGCC GCCGCTGCGC CGCGAGCCCG CCGGCTCGCT ACACCGGGAG 
CGCCAGAAGG AGCGGCAGAA AAAGCGGGGC GGTCGCTTCG GTACGTCGCA GAGAGCGGCA 
AAGACGGACG CATCCGCGGC GGAGGTGGCG GACCAGGCGG CGTTGGTGGT GGACACTGAG 
GTGTTTGACA ACGTGGTTGA ATCTCAGCTC GACACCGTCA GCAAGACCCG TGTGCAGTAC 
TACCTACGGT ACGAAAACCT CAAAGCACCG TCGATCACGT TTACCGCCAA CATGGGGCAC 
AGCGTGCTCC TGCATATTGC CACCTGGTCT CTCCCGGTAC CTGCCCGCTA CGGCAGCGAG 
ACGGAGGCAC CGAAGTGTCT GGAGTACGCT GTCAAGGGCG CCGCCTCGAC CCGGAGAGAT 
GCTGAACTGC TGTCGTGGAT GCATGCAGAG CGCACCCTCG ACTTTCTCGG CATCCCTCTC 
TTCCCCAACT TGCCAAAGTT GCAGGCGTAT CACGCCCAAC GTGCGCAGGT AGAGGGAAGG 
GTTGCCCCGG CTGTCGTCAC CGGGCCGACG GCGCCGCTGC CTCACCCCAG CGAAGTGACT 
GTGAAACCGC TGCGATTGCA CATGAGCTTC TGTCGTGTTG ATCTGACGAT TCCGCCACCG 
CCGTGTCCGT CGACGGACAT GGAAGGCAAC TCTTGCGTGC CCCCACATGA ATGGGAAGCC 
TACGTGGAGG CGTGCGCTGT GTACGTGCTT GCGAAGCAGA TGGCGCTGAA GAACAGCTTC 
TACGAGGAGC AGCGGGTGCC GCCGACGGGC GACGTCGTGA TCGATGCCGC CCTCGCGGAG 
GCGGAGGCGA AGCCCGTGGA TACAGACGCC CGATTGCGCT TTGCGGCGTA CTGCATGGCG 
ACGCTCGGCA AGTTCGTCCA ACGGTACGAC GCATATGTTG TCGGCCCAAT CCATCACCGT 
GTGACCTACG CCACAATCCC ACTCCCCGGC TTTGACTACC TGTTAGGGCG TGGTGTGGGG 
CCCAACAAGG TGGTGGCCAA GCGGCGAGCC GCCATGCACG CCTTGGCGAT CCTGCGGCGT 
GTCGATGAGT CGTACGACGA CACGTTCCAG GTGGTCAAGA GCTTCGTGCA GAGAGCGAAA 
GCCGCGAATA AGGTGAAAGG GGATGCCCAA GCGCATGCCG ATGACGAGTT CGACCTCGAC 
ACCTTCAGCA CCCTACCCCT CATCAACGCG GACGCCGAAA GCTCGCTGAC GAGGCAGGAA 
CGGCGCCACC TCGACCGCTT CAGCTCGCTC TTCGTTGCCC AGTCGGCGTG GTATTCGAAG 
AGCAGCTGTT TCACCTTGGA CGGCAAAAAG CGCGCCGTTC GCATGTACAC GGTGTGCTTC 
GATCTGCCGG CACCGGAGGA GCGTCAGCTG GTGCGTTTGT TGCCGAGAGA AGAGGGCGAA 
TCGGTGTGGA GGCGACAGCC CTTGGCGGAT ACGGTCAAGC TCGAGGTAGA AAGCGTTGAG 
GCGACGGCGC CAGACGCGAC TCGCGTGAAA ACGGGCGCCA TTTTTTCCGC GTGTGTCTCC 
ATCGTGGATG AGGATGGTCA GAAGCTGACA GCCAGCTGCA GTGGTGGTGG GCGCGTGGAC 
AACATGCTCA GCGCGTACGA GAAGCTGTTC ACGATGATGG ATAGGCAGTT TCCGGCAATG 
ATGTCGATCG TCGAGCTGCT GAAGCAGAAC CCGTATCTTA TGCCGGAGCT GATCCCTTCG 
CTGGCCATCC CTGATGCGGT GCGGCAGCGA ATGCAGGCGT GTCTGAGTAA TCAAAAAGAC 
TTGACTACGG CGATGACCTG TTCAGACAGA AAGGCGTCGT CGAAACGCCA CAAACCTAAC 
TTGGCCGCCA GCGATGAGGA GAAAACGGGC GGTGAGGCAG AGAACAGCTC CGCGGTTGCG 
CACCAGGCCT CCTACAGCGA GGCAGAGGAG TCTGCTCGCC TTGTGGAACA ACTGCAGCGG 
CGTATCACAA ACCCCGTATA CCTGGAGAAG TTTGCTACCA GGCGTGCGGA GCTGAGCATT 
GCAGAGCACA AGCGTGAAAT TCTCGAGGCG ATCCGCAGCA ACCCCATCAC CATTATCTGC 
GGCACGACAG GCTGCGGTAA GACGACGCAG GTGCCTCAGT ACATCCTCGA CGAAGAGACG 
CTCAGGGGTA ACGGGGGGCG CTGCTCCATC GTTGTCACGC AGCCACGGCG GCTCAGTGCC 
GTGTCGATTG CGCAGCGCGT CGCGGCAGAG CGTCTGGAGC CGCTAGAGGA GTCGACCGGG 
TACATGATCC GCTTCGACTC ACGCAAGGGT CGCCACATCA CCTTCGCCAC GACAGGCCTT 
GTTCTTCGGT TGATGCAGTC TGACGCGCTG CTTAGCAGCT ATACCCACGT CATCATTGAT 
GAAATCCACG AACGGGACAT GAACTCCGAC TTCATCCTGA TGCTGCTGCG GCAGGTGCTG 
GAGAAGCGAC GCGACATCCG CATTGTCCTG ATGAGCGCGA CCTTGCAAGC CGGTGACTTC 
CAGGCGTACT TTGGCGGTGC CCCGCTCATT CAGGTTGAGG GGCACATCTT TCCGGTCAAG 
GAGTTCTTCC TAGAGGACCT TGTTCCGTTC GCGCGCGAGC ACAACTGCAT GACGCCTCTG 
CTCAAGGAGG CAGCCGGCGT TGTGGGAAGT GACGGAGAGA GAGAAGGCAA TGCCGCAACG 
CAGCCAGAGG TTGGTGGCGC GCGTGTCCCC GTCGTGGTGT CAAACGTGGA CGACGCGGCG 
GCCAGCATCC CTCCCACACG CTACGGCTTT CTCGAGGCGT CGACGGCAAT CGATTATCCT 
ACCATTCAGT TCGCCATTGA GCAAGCGCTC CGCATGATCG ACATCGCCGA CTCCTCCATC 
CTCGTGTTCC TGCCCGGGTG GGAAGACATT CGCAAGGCTC GCGATGTGCT GGAGCGCAAC 
ACATCCTTTT ATGTACTGCC GCTGCACTCG GCCGTGTCAG CTGAGTCGCA GCTGAAGTGT 
TTCTTGCCGC CACCACCCGG CAAGGTCAAG ATTATCCTCT CCACGAATAT CGCTGAAAGT 
GGCGTGACCA TCGACGACGT CGGCGTCGTG ATCGACACCG GACGTATGAA GCAAGTCGCG 
TATTCTACGC GGATGCGGAA CGTTCTTTCA AAAACCGACT CGCGGGGCTA TGACTCGAGC 
CGAGTCCAAG ATGCTCTGGA TGTGACGTCG ACGCCTGGGG TGCCGGAGGA TGCACAGGGC 
AAGTTCTCGC ACTTGATGAA CATCTACGCG AGTCGCGCAA ACTGCGTGCA GCGCCGCGGT 
CGTGTCGGCC GCACGCGCCC TGGCCTTTGC ATTCGTCTTT TTTCGCGAGA GCACTTCCGC 
ACTTTGCACG AGTTTCAAAC GCCGGAGCTG CTGCGCACGC CGCTGGACAG AGTCTGCCTC 
ACCATCTTGA ATCTCGAGGT TGGTTCCCCT CAGCAATTCC TCAAGACCGC GATGGAGCCA 
CCCCTCGAGA CAGAGGTGGA GGGCGCCATG AAGCGACTAT ACGATCTCGG TGCCACCGAC 
GAAGACGGTC ATCTCACTCC ACTAGGGCGC CGCCTGGCAA AGCTGCCGCT AGATCCGGCG 
ACTGGAAAGA CCATTCTGCT CGGCGCCGTC TTCCGATGCC TCGACGCAGC CCTCACAATC 
GCAGCCACAG CTGAAAACGG CGTCTTCTCT CGCTCGTTCG ATGTGCGCGT CTCATCGCGC 
CTCCACCGGG AGGACCTTTC GTGCAACACC CTCTCCGACA TTCTCGCCTC CGTGAACGGG 
TACAACTACT GGGTCTCCCT GCATCGTGGA GGGTTGGGCG GGCAGGCGGC GGGGCAGATC 
AAGGCGCGGC ATCTGAGCGT TTCGGCGCTC ATGCAGGCCA CGCTGCTGAA GCAGCAGTAC 
TGCAACCTGC TCGTGGAAGA CGGCTTCATT GGTGAAGAAG CGAAAGTGCT GCCGTCGACG 
AACCGCTCTC GCTTCGGGAG CGATGATATG GTGTTTATCG AATCCTCCGA GCACTCGCGC 
AACTCGATGG ATGTTGGTCT GTTAAAGTGT CTGCTCAGCG CCAGCGCGCT TCCCAAAGTT 
GCGATGATTA CGGGACCATT CGTGTTGCGG ACCTTGTTTG AGAATTACAT TCCGATGATG 
AACGACAGTG TACTGAGGAT GAGCGGGCTG ACCGAGACGT CGAATCCGTT TGTGATCTAC 
GGCGGGCTCA TGAAAATAGC AGAGAAGGAG ACGCTCATGG CGCATCATCT AACCTCCGTC 
CCCCTCTGGT CAGTGCTGTT AATGAGCACG CGCGCGACCC GGATGGACTA CGATCAGGAG 
CTCAAGCTTG GCATCGTGAG CAACTGGATC TTTTTCCGCT CGAGCTACGC CACGATTGAG 
CTTGTGCGAC GATTCAAGGC GCTTCTTGAC CGCCGCCTTT CTCGCAAGTT TGACGATCCG 
ACGGACGCGG TCAACAATGC CCAGCTCGAC GAGCTGTGCG AGATTCTGCG GGAGCTTGCA 
AACATGCGCT ACCACCCAAA TCGCCTGCAG CCCCCGTCTG TGGTGTGGGG AGAGCAGGGG 
AAGCTTCTCT CCCCTGACAG CAACGCCGAC GACGACGCGG CTGGCGCCAC GAGCGAAACA 
GGAGTCGAAA GCTCTACGGA GAGCGGCCTA ACCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001686427.1
CDS	1..6699
Translation	MSAAGGSGTPRALWAHGVANTAKEAELLAAMHAEQMADLLGYHVFSLPSRQHKHAEAARAAGRYASSPEDKDSGPSTEQRLAQWPLPLRRVLTDEETEGGEWQLVNTATSLRHYISKEHTLLSPCLLDPKSRLRIEALFARAPHGCPSFAQQLRTERVRTLTASSAAGGGEEAIVARLLLPSQVTGITNSSIDLVASGKAMERSTAVLLACMHAELLLDAVGVAIFDDAVTQKAHAMAAWSHGRPAPLPGAKPKNPSHVHLPQPLKELVVAAAGDGRSRTSSRSAEEDWFHRHTIVTEQSGTFVETVVGESTAMEDITAFLHTHGAMRAAAPFLQVAIGSRVKSTVLLPLPDLYGIRGGVGIASNAQDADTLAAMHALDVLCMLGVPVKATAAMDAAWKRVRRERIPDVPCESADVSTPSPPARRCTAKKLATQVAIAAAALSEDFVSVDAAQASSCNHASTSTLPRRRAVKRARLASDASAPDDSIPQANATDTTAASPPNVPVVATLRETGEKRLAAVRAVVPKEFWDMQADSPDGYIMIAPGVPEGSAVAAYAITSPRKMDKSAKGRVLDYLATVGRRLDDVTVVRQALSEEETEGPPRHRCALKVPVPAVCGEARLALGEADTVADAENAACMHAELILDTLGVCLYTDSVKQKRHAAACAQWGRWAPSTPGEEQPSSTPSPPPLRREPAGSLHRERQKERQKKRGGRFGTSQRAAKTDASAAEVADQAALVVDTEVFDNVVESQLDTVSKTRVQYYLRYENLKAPSITFTANMGHSVLLHIATWSLPVPARYGSETEAPKCLEYAVKGAASTRRDAELLSWMHAERTLDFLGIPLFPNLPKLQAYHAQRAQVEGRVAPAVVTGPTAPLPHPSEVTVKPLRLHMSFCRVDLTIPPPPCPSTDMEGNSCVPPHEWEAYVEACAVYVLAKQMALKNSFYEEQRVPPTGDVVIDAALAEAEAKPVDTDARLRFAAYCMATLGKFVQRYDAYVVGPIHHRVTYATIPLPGFDYLLGRGVGPNKVVAKRRAAMHALAILRRVDESYDDTFQVVKSFVQRAKAANKVKGDAQAHADDEFDLDTFSTLPLINADAESSLTRQERRHLDRFSSLFVAQSAWYSKSSCFTLDGKKRAVRMYTVCFDLPAPEERQLVRLLPREEGESVWRRQPLADTVKLEVESVEATAPDATRVKTGAIFSACVSIVDEDGQKLTASCSGGGRVDNMLSAYEKLFTMMDRQFPAMMSIVELLKQNPYLMPELIPSLAIPDAVRQRMQACLSNQKDLTTAMTCSDRKASSKRHKPNLAASDEEKTGGEAENSSAVAHQASYSEAEESARLVEQLQRRITNPVYLEKFATRRAELSIAEHKREILEAIRSNPITIICGTTGCGKTTQVPQYILDEETLRGNGGRCSIVVTQPRRLSAVSIAQRVAAERLEPLEESTGYMIRFDSRKGRHITFATTGLVLRLMQSDALLSSYTHVIIDEIHERDMNSDFILMLLRQVLEKRRDIRIVLMSATLQAGDFQAYFGGAPLIQVEGHIFPVKEFFLEDLVPFAREHNCMTPLLKEAAGVVGSDGEREGNAATQPEVGGARVPVVVSNVDDAAASIPPTRYGFLEASTAIDYPTIQFAIEQALRMIDIADSSILVFLPGWEDIRKARDVLERNTSFYVLPLHSAVSAESQLKCFLPPPPGKVKIILSTNIAESGVTIDDVGVVIDTGRMKQVAYSTRMRNVLSKTDSRGYDSSRVQDALDVTSTPGVPEDAQGKFSHLMNIYASRANCVQRRGRVGRTRPGLCIRLFSREHFRTLHEFQTPELLRTPLDRVCLTILNLEVGSPQQFLKTAMEPPLETEVEGAMKRLYDLGATDEDGHLTPLGRRLAKLPLDPATGKTILLGAVFRCLDAALTIAATAENGVFSRSFDVRVSSRLHREDLSCNTLSDILASVNGYNYWVSLHRGGLGGQAAGQIKARHLSVSALMQATLLKQQYCNLLVEDGFIGEEAKVLPSTNRSRFGSDDMVFIESSEHSRNSMDVGLLKCLLSASALPKVAMITGPFVLRTLFENYIPMMNDSVLRMSGLTETSNPFVIYGGLMKIAEKETLMAHHLTSVPLWSVLLMSTRATRMDYDQELKLGIVSNWIFFRSSYATIELVRRFKALLDRRLSRKFDDPTDAVNNAQLDELCEILRELANMRYHPNRLQPPSVVWGEQGKLLSPDSNADDDAAGATSETGVESSTESGLTE

Target ORF information:

RefSeq Version	XM_001686375.1
Organism	Leishmania major strain Friedlin
Definition	Leishmania major strain Friedlin RNA editing associated helicase 2,putative;with=GeneDB:Tb927.4.1500 (REH2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001686375.1

ORF Insert Sequence:

ATGAGTGCGG CTGGCGGCTC GGGCACACCC AGGGCACTGT GGGCGCACGG AGTCGCCAAC 
ACGGCGAAAG AGGCTGAGCT GCTAGCCGCC ATGCACGCGG AGCAGATGGC GGACTTGTTA 
GGGTATCACG TCTTCTCCCT CCCATCAAGG CAGCACAAGC ACGCCGAGGC AGCACGCGCG 
GCAGGTCGCT ACGCGTCCTC GCCGGAGGAT AAGGACAGTG GCCCTTCGAC AGAGCAGCGA 
CTGGCGCAGT GGCCCCTGCC CTTGAGGCGC GTTCTGACCG ACGAAGAAAC GGAAGGCGGG 
GAATGGCAGC TTGTGAACAC GGCGACCTCC CTGCGACACT ACATCTCTAA GGAGCACACG 
TTGCTGTCGC CGTGCCTGCT GGACCCCAAG TCGCGACTCA GGATCGAGGC CCTGTTCGCG 
CGTGCACCTC ACGGCTGCCC CTCCTTTGCG CAACAGCTCA GGACTGAAAG GGTGCGTACG 
CTGACGGCGA GCAGCGCGGC GGGCGGCGGC GAAGAGGCGA TCGTGGCCCG GCTCCTCTTG 
CCATCACAGG TGACGGGTAT CACGAACTCC TCGATAGATC TCGTCGCCTC TGGCAAGGCG 
ATGGAGCGGT CAACGGCGGT GCTGCTGGCC TGCATGCACG CAGAGCTCCT GCTGGATGCC 
GTGGGCGTTG CTATTTTCGA CGACGCGGTG ACGCAGAAAG CGCACGCCAT GGCGGCCTGG 
AGCCACGGAC GCCCCGCACC ACTACCGGGC GCCAAGCCAA AAAATCCGTC GCACGTGCAC 
CTGCCACAGC CGCTGAAGGA GCTCGTCGTG GCGGCAGCAG GGGATGGTCG GTCTCGCACG 
TCGTCTCGCT CGGCTGAAGA GGACTGGTTT CACAGGCATA CCATTGTCAC TGAGCAGAGT 
GGGACCTTTG TAGAAACCGT TGTGGGCGAG TCCACCGCGA TGGAGGACAT CACCGCGTTT 
CTCCACACTC ACGGTGCCAT GCGGGCCGCC GCACCGTTTC TGCAGGTGGC AATCGGTTCC 
CGGGTCAAGT CCACAGTACT GCTGCCCTTG CCGGACCTCT ACGGCATTCG CGGTGGCGTG 
GGGATCGCGT CGAACGCGCA GGATGCTGAC ACCCTCGCTG CCATGCATGC ACTGGATGTG 
CTGTGCATGC TCGGTGTCCC GGTAAAGGCT ACGGCGGCGA TGGACGCCGC GTGGAAGCGC 
GTTCGCCGCG AGCGCATTCC CGACGTGCCC TGCGAGTCGG CGGACGTCTC CACGCCGAGC 
CCCCCGGCGC GCCGCTGCAC TGCCAAGAAG TTAGCCACGC AAGTGGCTAT AGCCGCAGCG 
GCCTTATCTG AAGATTTCGT GTCCGTGGAC GCAGCGCAGG CAAGCTCCTG CAACCACGCC 
AGCACATCCA CGCTGCCGCG GCGCCGTGCG GTGAAGCGAG CGCGACTGGC CTCGGATGCA 
TCCGCACCGG ATGATAGCAT ACCACAGGCA AACGCAACCG ACACGACTGC TGCCTCTCCG 
CCAAACGTGC CCGTGGTTGC GACATTGAGA GAGACTGGCG AGAAGCGGCT GGCAGCGGTG 
CGGGCGGTGG TGCCAAAGGA GTTCTGGGAT ATGCAGGCAG ACAGCCCTGA CGGGTACATT 
ATGATTGCCC CTGGCGTGCC GGAGGGATCT GCGGTGGCTG CGTATGCCAT TACGTCCCCG 
CGCAAGATGG ACAAATCGGC CAAGGGACGC GTGTTGGACT ACTTGGCGAC CGTGGGCCGT 
CGCCTCGACG ATGTCACCGT GGTGCGGCAA GCCCTCAGCG AGGAGGAGAC GGAGGGCCCA 
CCGCGGCACC GGTGTGCGCT GAAAGTGCCA GTGCCAGCCG TTTGCGGGGA AGCTCGCCTC 
GCGCTAGGCG AGGCCGACAC AGTCGCCGAC GCCGAAAACG CCGCCTGTAT GCACGCGGAG 
CTCATCCTGG ACACACTCGG GGTCTGCCTC TACACGGACT CTGTGAAGCA GAAGCGGCAC 
GCCGCGGCAT GCGCGCAGTG GGGTCGCTGG GCGCCTTCGA CTCCTGGCGA AGAACAGCCG 
AGCAGCACAC CGTCCCCGCC GCCGCTGCGC CGCGAGCCCG CCGGCTCGCT ACACCGGGAG 
CGCCAGAAGG AGCGGCAGAA AAAGCGGGGC GGTCGCTTCG GTACGTCGCA GAGAGCGGCA 
AAGACGGACG CATCCGCGGC GGAGGTGGCG GACCAGGCGG CGTTGGTGGT GGACACTGAG 
GTGTTTGACA ACGTGGTTGA ATCTCAGCTC GACACCGTCA GCAAGACCCG TGTGCAGTAC 
TACCTACGGT ACGAAAACCT CAAAGCACCG TCGATCACGT TTACCGCCAA CATGGGGCAC 
AGCGTGCTCC TGCATATTGC CACCTGGTCT CTCCCGGTAC CTGCCCGCTA CGGCAGCGAG 
ACGGAGGCAC CGAAGTGTCT GGAGTACGCT GTCAAGGGCG CCGCCTCGAC CCGGAGAGAT 
GCTGAACTGC TGTCGTGGAT GCATGCAGAG CGCACCCTCG ACTTTCTCGG CATCCCTCTC 
TTCCCCAACT TGCCAAAGTT GCAGGCGTAT CACGCCCAAC GTGCGCAGGT AGAGGGAAGG 
GTTGCCCCGG CTGTCGTCAC CGGGCCGACG GCGCCGCTGC CTCACCCCAG CGAAGTGACT 
GTGAAACCGC TGCGATTGCA CATGAGCTTC TGTCGTGTTG ATCTGACGAT TCCGCCACCG 
CCGTGTCCGT CGACGGACAT GGAAGGCAAC TCTTGCGTGC CCCCACATGA ATGGGAAGCC 
TACGTGGAGG CGTGCGCTGT GTACGTGCTT GCGAAGCAGA TGGCGCTGAA GAACAGCTTC 
TACGAGGAGC AGCGGGTGCC GCCGACGGGC GACGTCGTGA TCGATGCCGC CCTCGCGGAG 
GCGGAGGCGA AGCCCGTGGA TACAGACGCC CGATTGCGCT TTGCGGCGTA CTGCATGGCG 
ACGCTCGGCA AGTTCGTCCA ACGGTACGAC GCATATGTTG TCGGCCCAAT CCATCACCGT 
GTGACCTACG CCACAATCCC ACTCCCCGGC TTTGACTACC TGTTAGGGCG TGGTGTGGGG 
CCCAACAAGG TGGTGGCCAA GCGGCGAGCC GCCATGCACG CCTTGGCGAT CCTGCGGCGT 
GTCGATGAGT CGTACGACGA CACGTTCCAG GTGGTCAAGA GCTTCGTGCA GAGAGCGAAA 
GCCGCGAATA AGGTGAAAGG GGATGCCCAA GCGCATGCCG ATGACGAGTT CGACCTCGAC 
ACCTTCAGCA CCCTACCCCT CATCAACGCG GACGCCGAAA GCTCGCTGAC GAGGCAGGAA 
CGGCGCCACC TCGACCGCTT CAGCTCGCTC TTCGTTGCCC AGTCGGCGTG GTATTCGAAG 
AGCAGCTGTT TCACCTTGGA CGGCAAAAAG CGCGCCGTTC GCATGTACAC GGTGTGCTTC 
GATCTGCCGG CACCGGAGGA GCGTCAGCTG GTGCGTTTGT TGCCGAGAGA AGAGGGCGAA 
TCGGTGTGGA GGCGACAGCC CTTGGCGGAT ACGGTCAAGC TCGAGGTAGA AAGCGTTGAG 
GCGACGGCGC CAGACGCGAC TCGCGTGAAA ACGGGCGCCA TTTTTTCCGC GTGTGTCTCC 
ATCGTGGATG AGGATGGTCA GAAGCTGACA GCCAGCTGCA GTGGTGGTGG GCGCGTGGAC 
AACATGCTCA GCGCGTACGA GAAGCTGTTC ACGATGATGG ATAGGCAGTT TCCGGCAATG 
ATGTCGATCG TCGAGCTGCT GAAGCAGAAC CCGTATCTTA TGCCGGAGCT GATCCCTTCG 
CTGGCCATCC CTGATGCGGT GCGGCAGCGA ATGCAGGCGT GTCTGAGTAA TCAAAAAGAC 
TTGACTACGG CGATGACCTG TTCAGACAGA AAGGCGTCGT CGAAACGCCA CAAACCTAAC 
TTGGCCGCCA GCGATGAGGA GAAAACGGGC GGTGAGGCAG AGAACAGCTC CGCGGTTGCG 
CACCAGGCCT CCTACAGCGA GGCAGAGGAG TCTGCTCGCC TTGTGGAACA ACTGCAGCGG 
CGTATCACAA ACCCCGTATA CCTGGAGAAG TTTGCTACCA GGCGTGCGGA GCTGAGCATT 
GCAGAGCACA AGCGTGAAAT TCTCGAGGCG ATCCGCAGCA ACCCCATCAC CATTATCTGC 
GGCACGACAG GCTGCGGTAA GACGACGCAG GTGCCTCAGT ACATCCTCGA CGAAGAGACG 
CTCAGGGGTA ACGGGGGGCG CTGCTCCATC GTTGTCACGC AGCCACGGCG GCTCAGTGCC 
GTGTCGATTG CGCAGCGCGT CGCGGCAGAG CGTCTGGAGC CGCTAGAGGA GTCGACCGGG 
TACATGATCC GCTTCGACTC ACGCAAGGGT CGCCACATCA CCTTCGCCAC GACAGGCCTT 
GTTCTTCGGT TGATGCAGTC TGACGCGCTG CTTAGCAGCT ATACCCACGT CATCATTGAT 
GAAATCCACG AACGGGACAT GAACTCCGAC TTCATCCTGA TGCTGCTGCG GCAGGTGCTG 
GAGAAGCGAC GCGACATCCG CATTGTCCTG ATGAGCGCGA CCTTGCAAGC CGGTGACTTC 
CAGGCGTACT TTGGCGGTGC CCCGCTCATT CAGGTTGAGG GGCACATCTT TCCGGTCAAG 
GAGTTCTTCC TAGAGGACCT TGTTCCGTTC GCGCGCGAGC ACAACTGCAT GACGCCTCTG 
CTCAAGGAGG CAGCCGGCGT TGTGGGAAGT GACGGAGAGA GAGAAGGCAA TGCCGCAACG 
CAGCCAGAGG TTGGTGGCGC GCGTGTCCCC GTCGTGGTGT CAAACGTGGA CGACGCGGCG 
GCCAGCATCC CTCCCACACG CTACGGCTTT CTCGAGGCGT CGACGGCAAT CGATTATCCT 
ACCATTCAGT TCGCCATTGA GCAAGCGCTC CGCATGATCG ACATCGCCGA CTCCTCCATC 
CTCGTGTTCC TGCCCGGGTG GGAAGACATT CGCAAGGCTC GCGATGTGCT GGAGCGCAAC 
ACATCCTTTT ATGTACTGCC GCTGCACTCG GCCGTGTCAG CTGAGTCGCA GCTGAAGTGT 
TTCTTGCCGC CACCACCCGG CAAGGTCAAG ATTATCCTCT CCACGAATAT CGCTGAAAGT 
GGCGTGACCA TCGACGACGT CGGCGTCGTG ATCGACACCG GACGTATGAA GCAAGTCGCG 
TATTCTACGC GGATGCGGAA CGTTCTTTCA AAAACCGACT CGCGGGGCTA TGACTCGAGC 
CGAGTCCAAG ATGCTCTGGA TGTGACGTCG ACGCCTGGGG TGCCGGAGGA TGCACAGGGC 
AAGTTCTCGC ACTTGATGAA CATCTACGCG AGTCGCGCAA ACTGCGTGCA GCGCCGCGGT 
CGTGTCGGCC GCACGCGCCC TGGCCTTTGC ATTCGTCTTT TTTCGCGAGA GCACTTCCGC 
ACTTTGCACG AGTTTCAAAC GCCGGAGCTG CTGCGCACGC CGCTGGACAG AGTCTGCCTC 
ACCATCTTGA ATCTCGAGGT TGGTTCCCCT CAGCAATTCC TCAAGACCGC GATGGAGCCA 
CCCCTCGAGA CAGAGGTGGA GGGCGCCATG AAGCGACTAT ACGATCTCGG TGCCACCGAC 
GAAGACGGTC ATCTCACTCC ACTAGGGCGC CGCCTGGCAA AGCTGCCGCT AGATCCGGCG 
ACTGGAAAGA CCATTCTGCT CGGCGCCGTC TTCCGATGCC TCGACGCAGC CCTCACAATC 
GCAGCCACAG CTGAAAACGG CGTCTTCTCT CGCTCGTTCG ATGTGCGCGT CTCATCGCGC 
CTCCACCGGG AGGACCTTTC GTGCAACACC CTCTCCGACA TTCTCGCCTC CGTGAACGGG 
TACAACTACT GGGTCTCCCT GCATCGTGGA GGGTTGGGCG GGCAGGCGGC GGGGCAGATC 
AAGGCGCGGC ATCTGAGCGT TTCGGCGCTC ATGCAGGCCA CGCTGCTGAA GCAGCAGTAC 
TGCAACCTGC TCGTGGAAGA CGGCTTCATT GGTGAAGAAG CGAAAGTGCT GCCGTCGACG 
AACCGCTCTC GCTTCGGGAG CGATGATATG GTGTTTATCG AATCCTCCGA GCACTCGCGC 
AACTCGATGG ATGTTGGTCT GTTAAAGTGT CTGCTCAGCG CCAGCGCGCT TCCCAAAGTT 
GCGATGATTA CGGGACCATT CGTGTTGCGG ACCTTGTTTG AGAATTACAT TCCGATGATG 
AACGACAGTG TACTGAGGAT GAGCGGGCTG ACCGAGACGT CGAATCCGTT TGTGATCTAC 
GGCGGGCTCA TGAAAATAGC AGAGAAGGAG ACGCTCATGG CGCATCATCT AACCTCCGTC 
CCCCTCTGGT CAGTGCTGTT AATGAGCACG CGCGCGACCC GGATGGACTA CGATCAGGAG 
CTCAAGCTTG GCATCGTGAG CAACTGGATC TTTTTCCGCT CGAGCTACGC CACGATTGAG 
CTTGTGCGAC GATTCAAGGC GCTTCTTGAC CGCCGCCTTT CTCGCAAGTT TGACGATCCG 
ACGGACGCGG TCAACAATGC CCAGCTCGAC GAGCTGTGCG AGATTCTGCG GGAGCTTGCA 
AACATGCGCT ACCACCCAAA TCGCCTGCAG CCCCCGTCTG TGGTGTGGGG AGAGCAGGGG 
AAGCTTCTCT CCCCTGACAG CAACGCCGAC GACGACGCGG CTGGCGCCAC GAGCGAAACA 
GGAGTCGAAA GCTCTACGGA GAGCGGCCTA ACCGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

The genome of the kinetoplastid parasite, Leishmania major.
Science (New York, N.Y.)309(5733)436-42(2005 Jul)
Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ

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REH2 cDNA ORF clone, Leishmania major strain Friedlin

Gene

Clones

gRNAs

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