ORF » Species Summary » Leishmania major strain Friedlin » LMJF_35_5050 cDNA ORF clone

LMJF_35_5050 cDNA ORF clone, Leishmania major strain Friedlin

The following LMJF_35_5050 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LMJF_35_5050 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OLb06976 XM_003722850.1
Latest version!		 Leishmania major strain Friedlin conserved hypothetical protein partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LMJF_35_5050 ( XM_003722850.1 ) cDNA ORF clone, Leishmania major strain Friedlin -> XP_003722898.1 Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.
    Price & Availability↑
    CloneID OLb06976
    Clone ID Related Accession (Same CDS sequence) XM_003722850.1
    Accession Version XM_003722850.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 1497456000000
    Organism Leishmania major strain Friedlin
    Product conserved hypothetical protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_007284). COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGGTGG TGCCGAACGG GCACGAGGCC GAGGTAGACG CGGCGCTGGG ATTTCGGCGT 
    TTCCCAAATG CGCCGCACAT GACACACACC GTCTTCCGCC CCTCAAAGAA CCTGACCGCC 
    ACCTTTACCA CGGCTGCGTC TTCATCGGCC TTGGGCGTCC ACAATACCTA CCAAACCTTG 
    ACGCCGAATG CCCTTGCAAA CTTGCGCGTG CACAGGGGTC TCCGGGTCGA TGACTTTCCT 
    TCACGTCCGC ATACGCCACA AGACGCCAAG TCCGCCACTT CACTGCCACC GTTGCTGCCG 
    GCGGATCGTC TCTCGGTCGG GTCCAGCGAC GGCCTTCGCG AAAGAGGAAC TGAGGTGTTT 
    CGAGAGTGGG TGAAGGCGCC CACGAACTCC TCTACATCCT CGCCTAACGG CGCTGAGGGC 
    GGCGACGCAG ATGCGGCTGC GATCAACGGC AATGCGCACG GCTCTCTTGA ACTCGTCAGC 
    ACGTCAGCGC CGGCGGCTTC ACGGGGCACA GAGAGCGGGC AGAGTTGCAC CTCTGCGTTG 
    GTGCCGCCCG CGACGTTAGT CCCGGCGAAG ACGGCGTTCG CGTCGTCGGA TCACTGCCCG 
    TCGGTATCCG TCTCGCCGGA ATCGCTGCGT CTCGTACGGC CAAAGAGCTC AACGGCTTCT 
    TTCACCCCGT TGCAGGCACC GGAAGTATCC AGTGCGCCCA CGGTGGACGC AGCTCTGTAC 
    CGCTTACGTG ACGTACTGAT GCAGGCGCAG CGCGTACTGG ATAAATCACA GATATCCGGG 
    GATGCCCGTG CACTTCCCGC GCCGCCGGCG ATGCCACCCG AGCCTGCGGA GTCAGCACAG 
    CACGAACAAC AGACGGCAGT CCTTCTGGCC CAAACGTCCA CTGGCTCGCC CGCTAACGAG 
    GATGTGATGC CGGAGGACTC ACTAGATTGC GTATCGCCCC TCGCCAGCAC GCACGCAGCA 
    CTCTATGCTT TATCCGAAGC AGAGCGGCGA GTGGGTGTGC CTGGCGCGGC TTCGCCAACG 
    TTTGCCGCCG CTAAGGTGGC GGATAACGTC GCGGTGCCCG ACGAAACCCG CTTTATGGTG 
    TTGTGCGCCG TCAGTCAACC GTCAGCAATC ATGGAGCGCC GTCTCCGTCG CCGCGGACCA 
    GCAACAAGAG CGCCTATGGA AGGACTGACC TCTCCTCCGG CTCATTCGCT GTTTGGCGCG 
    TGCGCAGAGA CAGGGGCGAT GCGCACGTCT TCACGCCGCA TCGCCGAGGT GCCAAGCGCT 
    TCGGCCGCCG TGAGTGCATC CACTGTGGCG CAACTGACAA CAGCGGCAGG CCAACTTCAG 
    CGCTCGTCGT CGCCCAACGC AGGTGGCGGT GAGCATCCCA GCTTGCGCGC ACCCGCGCAG 
    CGAGGTTCAG CTGAGGGGTC CGTGAGCCGT CACGTGCACG AGGATCCGTC GCTGATTCCG 
    GGACTCGCCG AGTTTTTGCG GGCTGGAAAC CCCAACAGCT TTACTTCCCT CAGCGCAGCT 
    ACCCATGCCG CGCCGCCTGC ACGGGCGAAG GCGTCGCCGT CAACACGCCG CCCTCGCCGC 
    CGCTCTGGAC CCCCTGCCGC AGCCGATCCC TCTCCTGCTG TGTTGCTCGG TCATTCACCA 
    TCGTTGTTGC AACGCCGTGG CTCTAGTGGC ACCGCACTGT CTCAGGCGGA CCGTGCTGTG 
    ACCCCGTCGC TGCATCTGCC ATACGCGCAC ACAGAGGGGA GTGCGACAGT AGCGGACGTC 
    TCGCCACGCC GCGGATCTGC CCGTGCCCGC CGTGTAGACT CGACGCCTAG TTACGCGCAG 
    CCGACACTGA GCTGGCTTTC CAAGGGGTCT GAGGTTTCTG CTGACCAGTT CCCGATGAGC 
    CGCGTCGCCT CGCCGCAGAC GAGCCCCTTG AAGGACCCGG TGACAGCGTC GCGTGACGCC 
    GCACCAAGTG AACCGGCGCT ACTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003722898.1
    CDS1..1947
    Translation

    Target ORF information:

    RefSeq Version XM_003722850.1
    Organism Leishmania major strain Friedlin
    Definition Leishmania major strain Friedlin conserved hypothetical protein partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_003722850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGGTGG TGCCGAACGG GCACGAGGCC GAGGTAGACG CGGCGCTGGG ATTTCGGCGT 
    TTCCCAAATG CGCCGCACAT GACACACACC GTCTTCCGCC CCTCAAAGAA CCTGACCGCC 
    ACCTTTACCA CGGCTGCGTC TTCATCGGCC TTGGGCGTCC ACAATACCTA CCAAACCTTG 
    ACGCCGAATG CCCTTGCAAA CTTGCGCGTG CACAGGGGTC TCCGGGTCGA TGACTTTCCT 
    TCACGTCCGC ATACGCCACA AGACGCCAAG TCCGCCACTT CACTGCCACC GTTGCTGCCG 
    GCGGATCGTC TCTCGGTCGG GTCCAGCGAC GGCCTTCGCG AAAGAGGAAC TGAGGTGTTT 
    CGAGAGTGGG TGAAGGCGCC CACGAACTCC TCTACATCCT CGCCTAACGG CGCTGAGGGC 
    GGCGACGCAG ATGCGGCTGC GATCAACGGC AATGCGCACG GCTCTCTTGA ACTCGTCAGC 
    ACGTCAGCGC CGGCGGCTTC ACGGGGCACA GAGAGCGGGC AGAGTTGCAC CTCTGCGTTG 
    GTGCCGCCCG CGACGTTAGT CCCGGCGAAG ACGGCGTTCG CGTCGTCGGA TCACTGCCCG 
    TCGGTATCCG TCTCGCCGGA ATCGCTGCGT CTCGTACGGC CAAAGAGCTC AACGGCTTCT 
    TTCACCCCGT TGCAGGCACC GGAAGTATCC AGTGCGCCCA CGGTGGACGC AGCTCTGTAC 
    CGCTTACGTG ACGTACTGAT GCAGGCGCAG CGCGTACTGG ATAAATCACA GATATCCGGG 
    GATGCCCGTG CACTTCCCGC GCCGCCGGCG ATGCCACCCG AGCCTGCGGA GTCAGCACAG 
    CACGAACAAC AGACGGCAGT CCTTCTGGCC CAAACGTCCA CTGGCTCGCC CGCTAACGAG 
    GATGTGATGC CGGAGGACTC ACTAGATTGC GTATCGCCCC TCGCCAGCAC GCACGCAGCA 
    CTCTATGCTT TATCCGAAGC AGAGCGGCGA GTGGGTGTGC CTGGCGCGGC TTCGCCAACG 
    TTTGCCGCCG CTAAGGTGGC GGATAACGTC GCGGTGCCCG ACGAAACCCG CTTTATGGTG 
    TTGTGCGCCG TCAGTCAACC GTCAGCAATC ATGGAGCGCC GTCTCCGTCG CCGCGGACCA 
    GCAACAAGAG CGCCTATGGA AGGACTGACC TCTCCTCCGG CTCATTCGCT GTTTGGCGCG 
    TGCGCAGAGA CAGGGGCGAT GCGCACGTCT TCACGCCGCA TCGCCGAGGT GCCAAGCGCT 
    TCGGCCGCCG TGAGTGCATC CACTGTGGCG CAACTGACAA CAGCGGCAGG CCAACTTCAG 
    CGCTCGTCGT CGCCCAACGC AGGTGGCGGT GAGCATCCCA GCTTGCGCGC ACCCGCGCAG 
    CGAGGTTCAG CTGAGGGGTC CGTGAGCCGT CACGTGCACG AGGATCCGTC GCTGATTCCG 
    GGACTCGCCG AGTTTTTGCG GGCTGGAAAC CCCAACAGCT TTACTTCCCT CAGCGCAGCT 
    ACCCATGCCG CGCCGCCTGC ACGGGCGAAG GCGTCGCCGT CAACACGCCG CCCTCGCCGC 
    CGCTCTGGAC CCCCTGCCGC AGCCGATCCC TCTCCTGCTG TGTTGCTCGG TCATTCACCA 
    TCGTTGTTGC AACGCCGTGG CTCTAGTGGC ACCGCACTGT CTCAGGCGGA CCGTGCTGTG 
    ACCCCGTCGC TGCATCTGCC ATACGCGCAC ACAGAGGGGA GTGCGACAGT AGCGGACGTC 
    TCGCCACGCC GCGGATCTGC CCGTGCCCGC CGTGTAGACT CGACGCCTAG TTACGCGCAG 
    CCGACACTGA GCTGGCTTTC CAAGGGGTCT GAGGTTTCTG CTGACCAGTT CCCGATGAGC 
    CGCGTCGCCT CGCCGCAGAC GAGCCCCTTG AAGGACCCGG TGACAGCGTC GCGTGACGCC 
    GCACCAAGTG AACCGGCGCT ACTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
    Genome research21(12)2129-42(2011 Dec)
    Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC


    The genome of the kinetoplastid parasite, Leishmania major.
    Science (New York, N.Y.)309(5733)436-42(2005 Jul)
    Ivens AC,Peacock CS,Worthey EA,Murphy L,Aggarwal G,Berriman M,Sisk E,Rajandream MA,Adlem E,Aert R,Anupama A,Apostolou Z,Attipoe P,Bason N,Bauser C,Beck A,Beverley SM,Bianchettin G,Borzym K,Bothe G,Bruschi CV,Collins M,Cadag E,Ciarloni L,Clayton C,Coulson RM,Cronin A,Cruz AK,Davies RM,De Gaudenzi J,Dobson DE,Duesterhoeft A,Fazelina G,Fosker N,Frasch AC,Fraser A,Fuchs M,Gabel C,Goble A,Goffeau A,Harris D,Hertz-Fowler C,Hilbert H,Horn D,Huang Y,Klages S,Knights A,Kube M,Larke N,Litvin L,Lord A,Louie T,Marra M,Masuy D,Matthews K,Michaeli S,Mottram JC,Müller-Auer S,Munden H,Nelson S,Norbertczak H,Oliver K,O'neil S,Pentony M,Pohl TM,Price C,Purnelle B,Quail MA,Rabbinowitsch E,Reinhardt R,Rieger M,Rinta J,Robben J,Robertson L,Ruiz JC,Rutter S,Saunders D,Schäfer M,Schein J,Schwartz DC,Seeger K,Seyler A,Sharp S,Shin H,Sivam D,Squares R,Squares S,Tosato V,Vogt C,Volckaert G,Wambutt R,Warren T,Wedler H,Woodward J,Zhou S,Zimmermann W,Smith DF,Blackwell JM,Stuart KD,Barrell B,Myler PJ


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