LINJ_28_2960 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_28_2960
Entrez Gene ID	5070512
Full Name	putative heat-shock protein hsp70
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001470287.1	XP_001470324.1	putative heat-shock protein hsp70

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_28_2960 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_28_2960 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc04080	XM_001470287.1 Latest version!	Leishmania infantum JPCM5 putative heat-shock protein hsp70 partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_28_2960 ( XM_001470287.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001470324.1 Leishmania infantum JPCM5 putative heat-shock protein hsp70 partial mRNA.

Price & Availability↑

CloneID	OLc04080
Clone ID Related Accession (Same CDS sequence)	XM_001470287.1
Accession Version	XM_001470287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative heat-shock protein hsp70
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009412). COMPLETENESS: incomplete on both ends.

ATGACATTCG ACGGCGCCAT CGGCATCGAC CTGGGCACGA CGTACTCGTG CGTGGGCGTG 
TGGCAGAACG AACGCGTGGA TATCATCGCG AACGACCAGG GCAACCGCAC GACACCGTCG 
TACGTTGCGT TCACGGACTC GGAGCGCCTG ATCGGCGATG CCGCGAAGAA CCAGGTGGCA 
ATGAACCCGC ACAACACGGT GTTCGACGCG AAGCGCCTGA TTGGCCGCAA GTTCAACGAC 
TCGGTTGTGC AGTCGGACAT GAAGCACTGG CCGTTCAAGG TGACGACGAA GGGCGACGAC 
AAGCCCATGA TTGCGGTGCA GTACCGCGGC GAGGAGAAGA CCTTCACGCC GGAGGAGATC 
AGCTCGATGG TGCTGCTGAA GATGAAGGAG ACAGCGGAGG CGTACCTGGG CAAGCAGGTG 
AAGAAGGCCG TGGTGACGGT GCCGGCGTAC TTCAACGACT CGCAGCGCCA GGCAACGAAG 
GACGCCGGCA CGATTGCTGG CCTGGAGGTG CTGCGCATCA TCAACGAGCC GACGGCGGCG 
GCCATCGCGT ACGGCCTGGA CAAGGGCGAC GACGGCAAGG AGCGCAACGT GCTGATCTTC 
GACCTTGGCG GCGGCACGTT CGATGTGACG CTGTTGACGA TCGACGGCGG CATCTTCGAG 
GTGAAGGCGA CGAACGGCGA CACGCACCTT GGCGGCGAGG ACTTCGACAA CCGCCTCGTC 
ACGTTCTTCA CCGAGGAGTT CAAGCGCAAG AACAAGGGTA AGAACCTGGC GTCGAGCCAC 
CGCGCGCTGC GCCGTCTGCG CACGGCGTGC GAGCGCGCGA AGCGCACGCT GTCGTCCGCG 
ACGCAGGCGA CGATCGAGAT CGACGCGTTG TTCGAGAACG TTGACTTTCA GGCCACCATC 
ACGCGCGCGC GCTTCGAGGA GCTGTGCGGC GACCTGTTCC GCAGCACGAT CCAGCCGGTG 
GAGCGCGTGC TGCAGGACGC GAAGATGGAC AAGCGCTCCG TGCACGACGT GGTGCTGGTG 
GGCGGGTCAA CGCGCATCCC GAAGGTGCAG TCCCTCGTGT CGGACTTCTT CGGCGGCAAG 
GAGCTGAACA AGAGCATCAA CCCCGACGAG GCTGTCGCGT ACGGCGCGGC GGTGCAGGCC 
TTCATCCTGA CGGGCGGCAA GAGCAAGCAG ACGGAGGGCC TGCTGCTGCT GGACGTGACG 
CCGCTGACGC TGGGCATCGA GACGGCCGGC GGCGTGATGA CGGCGCTGAT CAAGCGCAAC 
ACGACGATTC CGACCAAGAA GAGCCAGATC TTCTCGACGT ACGCGGACAA CCAGCCCGGC 
GTGCACATCC AGGTCTTCGA GGGCGAGCGC GCGATGACGA AGGACTGCCA CCTGCTGGGC 
ACGTTCGACC TGTCCGGCAT CCCGCCGGCG CCGCGCGGTG TGCCGCAGAT CGAGGTGACG 
TTCGACCTGG ACGCAAACGG CATCCTGAAC GTGTCCGCGG AGGAGAAGGG CACCGGCAAG 
CGCAACCAGA TCACCATCAC CAACGACAAG GGCCGGCTGA GCAAGGACGA GATCGAGCGC 
ATGGTGAACG ACGCGATGAA GTACGAGGCG GACGACAGGG CGCAGCGCGA CCGCGTGGAG 
GCAAAGAACG GCCTGGAGAA CTACGCGTAC TCGATGAAGA ACACGCTCGG CGACTCGAAC 
GTGTCCGGCA AGCTGGACGA TAGCGACAAG GCCACGCTGA ACAAGGAGAT CGACGTGACG 
CTGGAGTGGC TGAGCAGCAA CCAGGAGGCG ACGAAGGAGG AGTACGAGCA CAAGCAGAAG 
GAGCTGGAGA GCGTATGCAA CCCGATCATG ACCAAGATGT ACCAGAGCAT GGGCGGTGCT 
GGGGGCGGCA TGCCCGGCGG TATGCCGGAC ATGAGCGGCA TGAGCGGTGG TGCGGGCCCG 
GCCGGCGGTG CGTCCTCTGG CCCCAAGGTC GAGGAGGTCG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001470324.1
CDS	1..1965
Translation	MTFDGAIGIDLGTTYSCVGVWQNERVDIIANDQGNRTTPSYVAFTDSERLIGDAAKNQVAMNPHNTVFDAKRLIGRKFNDSVVQSDMKHWPFKVTTKGDDKPMIAVQYRGEEKTFTPEEISSMVLLKMKETAEAYLGKQVKKAVVTVPAYFNDSQRQATKDAGTIAGLEVLRIINEPTAAAIAYGLDKGDDGKERNVLIFDLGGGTFDVTLLTIDGGIFEVKATNGDTHLGGEDFDNRLVTFFTEEFKRKNKGKNLASSHRALRRLRTACERAKRTLSSATQATIEIDALFENVDFQATITRARFEELCGDLFRSTIQPVERVLQDAKMDKRSVHDVVLVGGSTRIPKVQSLVSDFFGGKELNKSINPDEAVAYGAAVQAFILTGGKSKQTEGLLLLDVTPLTLGIETAGGVMTALIKRNTTIPTKKSQIFSTYADNQPGVHIQVFEGERAMTKDCHLLGTFDLSGIPPAPRGVPQIEVTFDLDANGILNVSAEEKGTGKRNQITITNDKGRLSKDEIERMVNDAMKYEADDRAQRDRVEAKNGLENYAYSMKNTLGDSNVSGKLDDSDKATLNKEIDVTLEWLSSNQEATKEEYEHKQKELESVCNPIMTKMYQSMGGAGGGMPGGMPDMSGMSGGAGPAGGASSGPKVEEVD

Target ORF information:

RefSeq Version	XM_001470287.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative heat-shock protein hsp70 partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001470287.1

ORF Insert Sequence:

ATGACATTCG ACGGCGCCAT CGGCATCGAC CTGGGCACGA CGTACTCGTG CGTGGGCGTG 
TGGCAGAACG AACGCGTGGA TATCATCGCG AACGACCAGG GCAACCGCAC GACACCGTCG 
TACGTTGCGT TCACGGACTC GGAGCGCCTG ATCGGCGATG CCGCGAAGAA CCAGGTGGCA 
ATGAACCCGC ACAACACGGT GTTCGACGCG AAGCGCCTGA TTGGCCGCAA GTTCAACGAC 
TCGGTTGTGC AGTCGGACAT GAAGCACTGG CCGTTCAAGG TGACGACGAA GGGCGACGAC 
AAGCCCATGA TTGCGGTGCA GTACCGCGGC GAGGAGAAGA CCTTCACGCC GGAGGAGATC 
AGCTCGATGG TGCTGCTGAA GATGAAGGAG ACAGCGGAGG CGTACCTGGG CAAGCAGGTG 
AAGAAGGCCG TGGTGACGGT GCCGGCGTAC TTCAACGACT CGCAGCGCCA GGCAACGAAG 
GACGCCGGCA CGATTGCTGG CCTGGAGGTG CTGCGCATCA TCAACGAGCC GACGGCGGCG 
GCCATCGCGT ACGGCCTGGA CAAGGGCGAC GACGGCAAGG AGCGCAACGT GCTGATCTTC 
GACCTTGGCG GCGGCACGTT CGATGTGACG CTGTTGACGA TCGACGGCGG CATCTTCGAG 
GTGAAGGCGA CGAACGGCGA CACGCACCTT GGCGGCGAGG ACTTCGACAA CCGCCTCGTC 
ACGTTCTTCA CCGAGGAGTT CAAGCGCAAG AACAAGGGTA AGAACCTGGC GTCGAGCCAC 
CGCGCGCTGC GCCGTCTGCG CACGGCGTGC GAGCGCGCGA AGCGCACGCT GTCGTCCGCG 
ACGCAGGCGA CGATCGAGAT CGACGCGTTG TTCGAGAACG TTGACTTTCA GGCCACCATC 
ACGCGCGCGC GCTTCGAGGA GCTGTGCGGC GACCTGTTCC GCAGCACGAT CCAGCCGGTG 
GAGCGCGTGC TGCAGGACGC GAAGATGGAC AAGCGCTCCG TGCACGACGT GGTGCTGGTG 
GGCGGGTCAA CGCGCATCCC GAAGGTGCAG TCCCTCGTGT CGGACTTCTT CGGCGGCAAG 
GAGCTGAACA AGAGCATCAA CCCCGACGAG GCTGTCGCGT ACGGCGCGGC GGTGCAGGCC 
TTCATCCTGA CGGGCGGCAA GAGCAAGCAG ACGGAGGGCC TGCTGCTGCT GGACGTGACG 
CCGCTGACGC TGGGCATCGA GACGGCCGGC GGCGTGATGA CGGCGCTGAT CAAGCGCAAC 
ACGACGATTC CGACCAAGAA GAGCCAGATC TTCTCGACGT ACGCGGACAA CCAGCCCGGC 
GTGCACATCC AGGTCTTCGA GGGCGAGCGC GCGATGACGA AGGACTGCCA CCTGCTGGGC 
ACGTTCGACC TGTCCGGCAT CCCGCCGGCG CCGCGCGGTG TGCCGCAGAT CGAGGTGACG 
TTCGACCTGG ACGCAAACGG CATCCTGAAC GTGTCCGCGG AGGAGAAGGG CACCGGCAAG 
CGCAACCAGA TCACCATCAC CAACGACAAG GGCCGGCTGA GCAAGGACGA GATCGAGCGC 
ATGGTGAACG ACGCGATGAA GTACGAGGCG GACGACAGGG CGCAGCGCGA CCGCGTGGAG 
GCAAAGAACG GCCTGGAGAA CTACGCGTAC TCGATGAAGA ACACGCTCGG CGACTCGAAC 
GTGTCCGGCA AGCTGGACGA TAGCGACAAG GCCACGCTGA ACAAGGAGAT CGACGTGACG 
CTGGAGTGGC TGAGCAGCAA CCAGGAGGCG ACGAAGGAGG AGTACGAGCA CAAGCAGAAG 
GAGCTGGAGA GCGTATGCAA CCCGATCATG ACCAAGATGT ACCAGAGCAT GGGCGGTGCT 
GGGGGCGGCA TGCCCGGCGG TATGCCGGAC ATGAGCGGCA TGAGCGGTGG TGCGGGCCCG 
GCCGGCGGTG CGTCCTCTGG CCCCAAGGTC GAGGAGGTCG ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_28_2960 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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