LINJ_20_1140 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_20_1140
Entrez Gene ID	5068661
Full Name	putative phosphatidylinositol 3-kinase
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001465208.2	XP_001465245.2	putative phosphatidylinositol 3-kinase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_20_1140 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_20_1140 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc02382	XM_001465208.2 Latest version!	Leishmania infantum JPCM5 putative phosphatidylinositol 3-kinase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_20_1140 ( XM_001465208.2 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001465245.2 Leishmania infantum JPCM5 putative phosphatidylinositol 3-kinase partial mRNA.

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CloneID	OLc02382
Clone ID Related Accession (Same CDS sequence)	XM_001465208.2
Accession Version	XM_001465208.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative phosphatidylinositol 3-kinase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009404). On Jul 14, 2011 this sequence version replaced XM_001465208.1. COMPLETENESS: incomplete on both ends.

ATGCAGCAGA TGCACTGGAC CGCGGCGCGC GGCCAGCTGT CTGGTCCTCA ACACTCGCAA 
CACCCTTCAC CGCAGGCATC ATCCACCCAC GCTAGTCAGA AGCAGCAGCA GCAGCAGCAC 
TTGCATCAGA ATGGGCCTCC GGGGCTGGCC GCGGCCGGCA TGGGCGGCGG CAGTGCAGGG 
GTGAATATTT CTTCAGGTCC CGCCGCCGGC GCGTCTGCTG TTCGCGCCAA CGTCAACAGC 
AACGCCTTTC ATAGCCCCCG CTTTTCGCAC CCCATGGGCC CGCTACCGCC CGCTCAGAAT 
CCGCAGCAGC AGGTCTTTTC CGTTTCACAG CAGCCTCCTG GTGCGCAGCT GCAGCAGCAA 
CAGCGCTCCC CTCGATCACT GTCAGGTCCG ACTCCCAATA ACGTCGTGAG TGGTAATAGA 
AGTGGCCTCG AGACGAGCGC GGCGCTCGCC GGGCGCGAGG CGCCGGCCAT CTCAGGCGCC 
AACTCCGCCG CCGCCCACAC AAGCTCTGCC GCAGTCGGAG TGTTCTTAAG CGGTACGCGC 
ACGAACTTCA CCGACTTCGG AGCGGCAGCA GCGTACGGAC TCGGTGCCGG TGGCGTTGGC 
TCGCCGAGCG GGCCCACCGC CAACGCCGGC AGCATCCTCG CAGATGCAAA TGATGAGGAG 
GTGAACCAGC TGTTACGGGA GCTAGAGTTC TCAGTGGACA GCCCTCACGC GTTGGCAGTG 
CTGCGAGACG CCGTGGACGC CATCGAAGCA CGGGCGCTGG CCAACTTTGA CCCTCTGTCG 
CGAGACACGT TCCTGGAGCA GCGCATCCGC GATGTCCTGA CGGAGCTGTC GCGAACGTCG 
AACGAGCGCT CCCTGCTGCT CGCCGTGAGT CTCATAGAGG CGTTGATCCC GATCGCCTAC 
ACCAGTCCTC ACTCCAAGTA CACACGCCTG TGCCGCAGTC TCTCGGTGCT GCGGCAGAGC 
GGGAATGAGA AGGCCGCCAG GGAGGCACAG CGGGTGTACA AGCTGCTGCT GGAGGCGGAT 
GCGCGCTACC CAGGGCCGGA GTCGCCGCTG AAGGCGTTTG TGCAGAAGGA GCTCCGCGAA 
AATTGCGAGC AGGCGCTCCT GCAACTCCAC GGTCGCGTGC AGCAGGCGAA AGCGGCCTTT 
CCGGCGCAGT TCTTTTCCGT CATGTTGGGC TGCGCCGCCA CTGCGGAAGT GAACCCGCGC 
TACATCTCTG GCAAAACTCG TGCGCAGGCG ATTCAGCTCG GCACGATGCT CACAGGCAGC 
CCCGATCCGC TGCTCCGCAA GGCTGCCTAC GAGTGCCTCG TCGCCATCTT TCGAAACACG 
AGCAACATGC GACCCAACGA GCTGGTTCTG GAGAGCCGCC GCCTCTGCGA TGAGTGCATG 
CGAACACTGA ACGCTAGTCA GCACAACGAG GGCCACGTTG TATCGGCTCT GAAGGCGCTG 
CACGCCCTCC TCAGCAGTCG CGGGCTCTCG GCGATCGACA GACAGGTGAT ACCGCAGCTG 
TGCGTGCTCG TCACGGAGCA GCACCGGGTG TCCAAGTCGC TACTGGTGCG CAGCGCAGTG 
TGCGACCTTG TGCCCATCAT TGCGCCGGTC GACATTGCGT CTATGCCGCG CCGCACCACG 
TACTGCGCTA TCATCATGGA GCCAGTGAAG AACGTGCGCG ATGAGCGCTT CAAGGCGCAC 
GAGCTGCATA ATGTGGCTTC CTTTGTCCGC AGTGTCGGGT ATCGCGTGCT TGACGCTACA 
AATCGCACAA ACTTGGAGTC GATCCTGCAC CGCTACATCT CCCGTCCGGA GACGCAGGAG 
GCTTGCTGGT CCATCATGGC TGCCATCTGC GCATCCTCGG CGTCGGCCAG CGGAGGCGCG 
GCATCGGTGG CGCCCATGGG TGGGCGCCGC GTAGACTACG TCAGTACATC TACCACACTC 
CCGAGCCCCA CAACGCAGAA CACCCATCAG GACCTCTCCT TGACGCCCTC CATGGTTGAG 
GGGCGCAAGG CCTCGCAGCA CTCCAGCCGC GAAAACTTAG CAACCTCGCC GTCGGCGCTT 
CAGCGGCACT TCTCGTACCA CCACGGTGAC GACAACGACG ATCAGGTCAG CGTTCCACTT 
GTCTCCGTTA GGACCACCTC ACAACGGTCG GCAGGCACGT GCTCCCTCAG CGCCTCCCCT 
ACCTCGACGA ACGCACCAGC CTTCAACCTC TCCACCTTCA GCGTACACCA CACTGTGACC 
ATCGACGACG TTGTCCTGCG CTGCCTGCCG TATCTCGCCC ACGCCATCCT GACCCAGTCA 
CTAGTAGACT CCCTTCGTGT AATACAAGCC TCGTGCCCGC AGTTGCGCGC TGATGTGCAG 
ATGGCTCTGG ACAAGCTGGT GGATAGCGTG CTGAAGGAGC CTTTCGCGCC GTCCGCGACG 
GCGGCGGCTG TGACACTGCC CCAGTATTTT TCCCAGCTGC CAAGCTCCAC GGCGCAGCAG 
CAGCAGCAAG CCCACTCCCT TCATAGCCGT GACGGTGGCG ATGGGAGCGC CGGTGGCTTT 
TCGAGCGCAG CAATGAACAG TGCCTCCCGC ACCGCTGGGT CTCACCCGCT CAGCCCCGCT 
AGTGGCCCGG AGCCGGTCAT GACGCTATCT CGTCCGGGGA CATGCAGCAC GCCGATCGGT 
GTCGGGGCGG TGGGGCGGCT CACACAGTAC TTTCGGCCGA GCGAGAGCGC GGCAATTCAC 
CGAGGCGAGG GCAGCACGAG CAGCCCGACC CCGACCTCCC CGGTGGGAAT GCGCAACACC 
TCTGGCCCTT TACCCTCGGG GAACAACAGC TTCCTGGGCC CCGACAGCGC CTACACCGTC 
GGTGGCGGCG ACTCGAACAG CGGAGCTCCG TACGGTGCTG GCGGACCACT TCGCTGCTCC 
CCGCTCTCCA CGTCGATGAG CGCGCCCTTC AGTCCCGTGA CGCACATGGC GGCAGGTGGC 
AGCGGTGCAG GTGGTGGAAA CGCCGCAGCA GCCCCGCCGT CGGTGCTCAA GAACGACTTG 
ACGGTGGCGC TGAAGGTGTT CACGGAGCGG CCCCTAACGT CGGTGCAGCA CCTGCATGAT 
CTTAAGAACT CGGTGATCGC CTTCCAGCGT CACGAGGATC CGCTCGTGCG GCAGCAGAGC 
AGCGAGACGA TCATTTCGAG CTTGCGGCAG TGGATAGCCT ACGCAAAGGA GCACAAAACC 
TCAACCTACT CCACACGCGT GACAGAGATA TTGGAGGCGT ACATGAAGAA CGTCCTGATG 
GAGGTCGACC GCCAGTGCGG GTTGTACGAG ATTACGCTGC TGGCAAACGC CACGAACCTG 
CGCGCCTTTT TATCAGAGCA GCGCATCCTA TCCTCGCTGC TGAGTTTCCT GAACAGCACA 
GCGCTGGTAC GCGAGAAAAC GGTGGAGCTT CTCGTCGCCA TCACGCAGGA CTCCAAGCCC 
TCCCCCTCTG TCGTGGCGGT GCAGCAGAGC CTGTACGTCA CCGTCGAATC GTGTGTGGTG 
GTGCTCGAGT ACACGAACGA CGTGAGCATG CTCCTGCGCA GCATGGCCGA CCTGCAGACC 
TTTACCCGGC TGTGCATCCG TCAGCTTAAC AACCACCTCA GCCGCATCTT CTACGCCTTT 
CGCAAGCGTC TTTTAGAGGT CAGCGTGCCT GACGTGGTGC TGCTTTCCAT TCTCAAGACA 
CTTGAGATCA TCCTGCAGGC ACTGGCGAAA GACGAAAAGA CCATCTTGCA GTACCAGGAC 
GACGTGCTAG AGCTCTACGA CCCGGTTGTG GAGATACTGA AGCAGTCGCC GTCGCCGACG 
CTGAGCCATG CTGCCATCAA CGTTCTCGTG TACGTACACG AAATGTGCAT CCCGAGCGCC 
TCGACGGATC TGCGTAAGCA GCAGGAGCTG ATCACCGCGC TGACACACGT CTACACCAGC 
AGCACCTGCA CTGTGAACGA ATCTCTGAGT ATGCTGACGC TGTTTGGCCA GCTTGGCGCC 
ATCGACCCCT CCGCACAGTC CGTTGCGACA ACCGCAAAGA AGAAGGAGGA GGCCGTCATC 
CAGGATGAGG CTGACCTGGA GCTCACCTAC GACTACACGG TGATTGTGTA CCGCACGCTG 
AGTCGCATGC TGGACGCGTC ACTCTCCGAT GCCGTCTGCT CTCAGGCTCT GCGCACCCTG 
CTCCAGTTCA TCCGCTTCAC TCAAGACAAG AAGGACCTCG TCGGCGGCGT CCACGCCGTT 
CGGGCGGTGC TGCAGATCAC AAAGCGCGTG ACGGACAGCC CGGCCCTGCG GGTGGAGGCG 
CTGCATGTAT TGGCGGCCGT TACCGCCATG CGGCATGAGC GTATTGTACG GCTCATGTTG 
CCTGAAATCG TGGTCCTGCT GGAGCAGCTG TGGACCCCGA AAGACCGCCT CCTCTTCCGC 
GCTGTCCTGG AGGTGGTGAG CGCGCTCAAG CCCGGCAAGC TGTCCGGCAA GGAGCAGTCG 
GAGTCGTGGG CGTGGCTGTA TCCGCGGCTG GTGGACGTGG CGCTGCAGGA CCGCACTGAG 
TCGCGCGAGT TCTGCCTCCG CGTTGTGGAG ATTGTGCTGA ATGCCAGCTA CATTCCGCCG 
CACTGCATCC CCGTTGTGTT TCCGATGCTA CTTCAGTTCC TGCAGCAATC AGACCAGCTG 
GTGGATGTGC GCAGCCAGTC CCTGTGCGCC GCCGTGCACA TCGTGTGCGA GCTGAAGGCG 
GTGCAGTTCT TTTCGTCGCT CCTGCACGGC ATCTGCACCC TCACGCGGCA CTGCGAGCTG 
AACGAAGATC TCGGCCCGCG CATCTCCACC CCAGGGGTGC GGGAGTCACT CAAGGTCTTG 
GCGGCACTGC ACCCCAGCGG CCGCAGCACG ATCAAGATGT TGCGTGACCG CATCGGCGAG 
GGGGATGAGC GCATGACGAA CCCGACCGGG TCCTCCATAT TCAACACCAT GGGCCTGCCA 
GCGACTAGTG TCACGAGTCT CGCCGGCGGC GGCTCCACCT CCTCGATGCT TGTCATGGGG 
CCAGCGACTG GTAACGCCAG CGGCGGGCAG GGTGGCGGAC TGGGAGTAGA AAATCCAGCG 
AATTCAGGGA TTGGTCCGCT AGTCAATATC GGCGGCTTTG GCGCCTCTAC CGCTGGTGGC 
GGCGCAGCAG CAGCGGGCGG GGCGGCTGGT GGTGGGTCGT ACCTGCGGCT CACAAACGTG 
TCTGAGACCT TTGCAGGGGC TGAGGAAGAG GACGTAGGGC TGACCCTCAG CGCAGACACG 
GCAGGCCAGG AAGTCGACCT CTTCATGAAG CATGTGGAAC TTGGCTTCCG CCTCAAGGAG 
AACAAGTTGC GCGAGTGGTT CGCCGAGTTC CAGAAGTACA TCATTCTCAT CTCCCCACAC 
CCGGCGTTCC GCATGATGGT GGACCTGCTT GACAAGCATG AACCCCTTCG CCGCGGGCTC 
TTCTTGCCTT CGTTCAAGTG CCTCTACGAG TCCCTGTCGG CTGACCAAAT CAAGAAGCTG 
AACGAGATGC TGACCCTCGT GCTGAACTGT TCTGATCACG AGCTGTCGAG CAAGTGTCTC 
GGGCTTGCTG ACTACCTCGA CCATAACGAG CCGAACGTGA AGCCAGAGGT GCTGGAGGTG 
GTGCGGTCGA TGCGGCGAGT CGACTATCAT CGCGTCAGTC ACCCCTCCGA GAACGGGTCG 
CTTCTTCTGC GCAGCACGGA GAGTAGCACG AACAACGTCG TCTCTCTGCG CACCCGCAGC 
TCCGCGTCAG CAGGGCTTCC GAAGGCGGAG TTCTCTACTT CCAGTGACGC TCCCATCGAT 
GTGCTGCCCA TGGCAAAGGT GCGCGGACGG AAGGCGCCGG TTTTCCAGCG CAGCTCAAAC 
AGCAGTGCCG GGGTAGCAGG CGATGAGTCC GGGACGGCAG GTGCCGAAGC CAGCAGTGGT 
GCCTCAACGG CACTTGCCAG CCCCAAAAAT AGCGCAAACG GTCGCCGCAG CGCTTCTCGC 
CGCGATGTGG AGATGTACGA GTTCACCTCC TTTGACGGCA ACGAGAATGG CGCCACCGAG 
TCTGGACGGC AGCAGTCGCA GCCCATCGCC TTGCCGACGT CGGAGACACC GGCCCAGGAA 
GAGGAGGCGG AGCGGCGCAG CTGCTCGCCC GTGGACACGC TCGATGGGGC AGAGAGCGAG 
AACAAGGCGG AGGACGTGGT GGCCCCCGTC AGCACCGACC ATCCGTCGGT TGGAGTGCTA 
GGCGCGGCGG GAGTGCATGT CATGGGAAGC TACAGGCCCC TTGTGAACTC CGTCGTACGT 
CGTTGCGACT CGAACGCGCC GCAGCAGTCC GGGGTGACCA GCCAGTTTCA CATCATCAGC 
ACCGCTAGCA CTCACAGTAA CGACCTCAGC AGCGACAGCC GTGACGGTGA TCTGCCGCCG 
GCCGGGGCCC ACATCCCGGC GCAGCCAAAC ATGCTCTTCT CGACGGAGGA CATCGTCCGC 
GCTGCAGAGA GAACCCGTAT GTACGACAAG GCACTCAGCT ATCTGGAGAA CCGTCTTCTG 
GCCATGCTCA ACCTCTACCG GTACACGAAG ATGCCACGAG AGGTGATCCA CAAGACGGTG 
TGGCCGCTGG CATGGCTCTA CAGCCAGCGT GAAATGCAGG ACTCCGTCGT AGGACTCTTC 
CGCGCTATCC GCTACGAAGG CGGCGAGGAC CAAGCAGGCT TCGGCTACGA GCTGCTCTGC 
TGGTGGAGTA AGGCGCAGAA CACGTACGCC AAGTCCATCA CGCAGCAGGC GATGCGCGAT 
ATGACGGTGC CGCCGCACAT TCTTGAAGGC TACGTGCGCA CCCTCACCCT GTGCGGTGAG 
TGGAGCCGGG CCTATGACGT GGCCATGCAG GTCTGTTCAC GGCCGGAGGC GTACGTTTCC 
TCGACGGTCG CGCGCTGCGG TGCAACGGCC GCGTGGCTGC TCGGGCGGTG GGATGACGTG 
CAACACCTCG CGGAGCGGCT CTCCATGTCG GAGCAGCACA CCACGGCGCT CCGTCTCTTC 
TTTCTCAACG GCGTGGCGCT GAAGAGCGCC ATTACGGAGC ACAGCAGCGC CGCCTACGAG 
AACCTGCGAC ACATGATCAC ACAGTCGAAG CTCGTGATTG ACGAGAGCCT GCGCACTCTT 
CTCCCGCTCA GCTACACGCA TGCCTACGAG AGTCTGACAA TGCTGCAGCA CTTCACGGAG 
ATGGAGGAGA CTATCGACTA CTGCCACTGC CGCTCTACCA AGGGTCGCCA GCAGATCTAC 
GAGCGGTGGA ACAACCGGTT CCGCTCTCTG AATCCGGATT CCCTGCAGCC AAGCCTGCTC 
TCGCTGATGC TGCACTCGCT CGTGCTCAGC ACAGGCGAGA TGGCGGACAT GATTCTGCAC 
TTCTGTGAGA CGCGGCAGTC GAGCCACCCG CAGCTGGCGG AGTGGGCCAT GAGCTGGCTT 
CGACACGGCT CCTTCAGCGG GCGCAGCAGC AACCTCTGTA GCCCGAGTGC GGCATCGCCG 
GCGTCACCGG GGCCCGTGGA GGGCATCACG GTGGCGACGC TGTCATCGGT GGACAAGAAT 
CCAAAGGTGG CTGTCGGCTT CATCACGCAT CTGTGGAGCC AAGGAAAGCG GCAGGCCGCT 
GTTGAGTCCA TGGAGATGTT TCTGCGCGAC TGCGGCAAGG ACCTGGAGGA GAACAACGCC 
ACCTGCTACG GCGACGCGCA GCTGCGGCTC GGCACGTGGA AACAGGAGCT GCACGCCGAC 
TCCTTCTGGG AGCGCGGCTT CCGGGACGAG GAGCTGGGCC ATTTTCACAA GGCTATCCGT 
GCCGTCCCGT CGAGCTACGA GGCGTGGCAC AGCTGGGGCC TCATGAACTA CCGTATCCAG 
CAGCGCGACC ACAACCTCTC CCCCGAGGAC CAGCGCACCT TTGTGGAGGC GGCGCATCAG 
GGCTTTGTTG GCGCCATATG CCGCTGCAAA GACTCCTCCG AGGCGCTGCC GGCCGTCATG 
CGTCTGCTGC AGCTGTGGGT CATCCACAAC GGAGTGGAGC TGCTGAAGGA GACGGTGGCC 
GACAGTATCT CGCGCATCCC GATCGACCAC TGGGTGCAGA CCATTCCGCA GCTGATCGGC 
CACCTCAGCA GCGACAGCCA CGACATCCGC GAAGTCATTG GCATGATCCT GCGCTCGCTC 
TGCGAGGTGC ACCCACAGGC CACCGTCTTT CCGCTCCTCG TCGTGATGAT GTCGGACGCC 
AGCACCAGCG GCAGCAATCA CAACGCCCTC AACAGCAACG GAGATGAGAG TAATATAGTG 
TCGGGTGCGT CCGCCATGTC CGATAGCTCG AATCCGCTGG CGCGGAAGCA GGAGATTGTG 
CAAGGCATCA TCCAGCACTG CCCGAAGCGC ATCTTCCACG AGGCCGAGGC GGTGGCAAAG 
CTGCTTGTGG ACGTGTCCGC CATTCCCATC GAGAAGATTC GCGAGAACCT GAGCCAGGTC 
GCTGCTGCGT GGAACCCAAA CGCCGAGTAC GAGGAGGACC CGGAGGAAGT GTACCGCCGC 
CTGCAGAACA CACTCGACAT CTTCCACGCC AACCGGCGCC ACCTTCTCTT CACCGTCGGT 
GACATTGGTC AGTTTGTGCA GATGGTCATG GAGGACGACC GCAACGGACA GCGAGAGAAG 
GCGGCGGGCG TCGTCAGCCA ACTCATCGAA GAGATCTCGA AGCATGTGGC GGAGAAGCTG 
GGCCGTGAGC CTCAGAAGGC GATGGAGCCG CTGCTGCATC TGCGTAATCT GTCCGTCGCG 
GTATTCGGGG AGTACGACGG TCACTGCCGC GACCATTACC CGACTATCGC ATCCTTTAGC 
TCCAAGCTGG ACGTCATCCC GTCGAAGAAG CGGCCGCGCC GCATCCAGCT CAACGGCTCG 
GACGGCTGTC TCTACACATA CTGCCTAAAG GGCAACGAGG ACATTCGCAT GGACGAGCGT 
GTCATGCAGC TGCTTGGAAT GGTGAACGTC CTACTGAGCC ACACCCGTAT CTCAAGCAGC 
GCCTACATCC ACCGCTTCCC TGTCATCCCG ATCAGCTCCA ACGTGGGGCT TCTTGGCTGG 
GTGGAGAACG CGAATACAAT CAACAACACC ATCTGCAACT ACCGTACCAA CATCTCGAAC 
GTGCGCACGC ATCAGGAGTC GAGTGTGCTG CGCGCCTACG TAGGGTCGTT CGGCAACTGG 
GACAAGCTCA GCCTCATTCA GCGCACGGAG ATACTCGACT TTGTGATGCA GAGCGAGCAC 
TGCGAAGCCG TTGACGTTTC TCGAGCAATG TGGCACCGCG CCAACACGGC GGAGCAGTGG 
CTTGACCGCC GAACTGCCTT TACAGTATCC TTGGCGACCA TGTCCATGGT GGGCTACGTG 
CTCGGCCTCG GCGATCGGCA TCTCGGCAAC ATCCTCATCT CTATGTCCTC TGGCAAGATT 
GTGCACATTG ACTTTGGAGA CAGCTTCGAT GTCGGTCGGC TACGCCACGT TCTGCCGGAG 
ACGATCCCGT TCCGCCTCAC GCGCATGCTG ACGAATGCGA TGGAGGTCTT CGGCGTGGAC 
GGCGTCTTCC GCGCCTCCGC AACGCGGACG CAGACGACGC TGCACAAGAA CCGTGATAGC 
ATCATGGCCC TGCTCTCCGC CTTCGTCCAC GACCCGATTG TGCAGTACAA GGGCAAGATG 
AAGAACATCA TGGAGAAGAG CCGGTCGCCG CAGGACATTG CAGAGCGCAT TCGTAACAAG 
CTGCGCGGGA TGGAGATGGC GATTGACCAC AGCAAGGTGA ACATCTTTAA CACGACGCAA 
GACAGCTGCC GCCGGCCAGA CCTCCTGTAC ATGTCGACCG CCTTCGATGA TACTGCCGTG 
CGGACACAGT CGTTGGCGAT GACGCCGGAG GAGCAGGTTA GCTTCTTGAT TGACGAGGCG 
ACGCGCATCG ACAACTACAC GACGATGTAC TTCGGCTGGG GCCCGCTGTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001465245.2
CDS	1..9894
Translation	MQQMHWTAARGQLSGPQHSQHPSPQASSTHASQKQQQQQHLHQNGPPGLAAAGMGGGSAGVNISSGPAAGASAVRANVNSNAFHSPRFSHPMGPLPPAQNPQQQVFSVSQQPPGAQLQQQQRSPRSLSGPTPNNVVSGNRSGLETSAALAGREAPAISGANSAAAHTSSAAVGVFLSGTRTNFTDFGAAAAYGLGAGGVGSPSGPTANAGSILADANDEEVNQLLRELEFSVDSPHALAVLRDAVDAIEARALANFDPLSRDTFLEQRIRDVLTELSRTSNERSLLLAVSLIEALIPIAYTSPHSKYTRLCRSLSVLRQSGNEKAAREAQRVYKLLLEADARYPGPESPLKAFVQKELRENCEQALLQLHGRVQQAKAAFPAQFFSVMLGCAATAEVNPRYISGKTRAQAIQLGTMLTGSPDPLLRKAAYECLVAIFRNTSNMRPNELVLESRRLCDECMRTLNASQHNEGHVVSALKALHALLSSRGLSAIDRQVIPQLCVLVTEQHRVSKSLLVRSAVCDLVPIIAPVDIASMPRRTTYCAIIMEPVKNVRDERFKAHELHNVASFVRSVGYRVLDATNRTNLESILHRYISRPETQEACWSIMAAICASSASASGGAASVAPMGGRRVDYVSTSTTLPSPTTQNTHQDLSLTPSMVEGRKASQHSSRENLATSPSALQRHFSYHHGDDNDDQVSVPLVSVRTTSQRSAGTCSLSASPTSTNAPAFNLSTFSVHHTVTIDDVVLRCLPYLAHAILTQSLVDSLRVIQASCPQLRADVQMALDKLVDSVLKEPFAPSATAAAVTLPQYFSQLPSSTAQQQQQAHSLHSRDGGDGSAGGFSSAAMNSASRTAGSHPLSPASGPEPVMTLSRPGTCSTPIGVGAVGRLTQYFRPSESAAIHRGEGSTSSPTPTSPVGMRNTSGPLPSGNNSFLGPDSAYTVGGGDSNSGAPYGAGGPLRCSPLSTSMSAPFSPVTHMAAGGSGAGGGNAAAAPPSVLKNDLTVALKVFTERPLTSVQHLHDLKNSVIAFQRHEDPLVRQQSSETIISSLRQWIAYAKEHKTSTYSTRVTEILEAYMKNVLMEVDRQCGLYEITLLANATNLRAFLSEQRILSSLLSFLNSTALVREKTVELLVAITQDSKPSPSVVAVQQSLYVTVESCVVVLEYTNDVSMLLRSMADLQTFTRLCIRQLNNHLSRIFYAFRKRLLEVSVPDVVLLSILKTLEIILQALAKDEKTILQYQDDVLELYDPVVEILKQSPSPTLSHAAINVLVYVHEMCIPSASTDLRKQQELITALTHVYTSSTCTVNESLSMLTLFGQLGAIDPSAQSVATTAKKKEEAVIQDEADLELTYDYTVIVYRTLSRMLDASLSDAVCSQALRTLLQFIRFTQDKKDLVGGVHAVRAVLQITKRVTDSPALRVEALHVLAAVTAMRHERIVRLMLPEIVVLLEQLWTPKDRLLFRAVLEVVSALKPGKLSGKEQSESWAWLYPRLVDVALQDRTESREFCLRVVEIVLNASYIPPHCIPVVFPMLLQFLQQSDQLVDVRSQSLCAAVHIVCELKAVQFFSSLLHGICTLTRHCELNEDLGPRISTPGVRESLKVLAALHPSGRSTIKMLRDRIGEGDERMTNPTGSSIFNTMGLPATSVTSLAGGGSTSSMLVMGPATGNASGGQGGGLGVENPANSGIGPLVNIGGFGASTAGGGAAAAGGAAGGGSYLRLTNVSETFAGAEEEDVGLTLSADTAGQEVDLFMKHVELGFRLKENKLREWFAEFQKYIILISPHPAFRMMVDLLDKHEPLRRGLFLPSFKCLYESLSADQIKKLNEMLTLVLNCSDHELSSKCLGLADYLDHNEPNVKPEVLEVVRSMRRVDYHRVSHPSENGSLLLRSTESSTNNVVSLRTRSSASAGLPKAEFSTSSDAPIDVLPMAKVRGRKAPVFQRSSNSSAGVAGDESGTAGAEASSGASTALASPKNSANGRRSASRRDVEMYEFTSFDGNENGATESGRQQSQPIALPTSETPAQEEEAERRSCSPVDTLDGAESENKAEDVVAPVSTDHPSVGVLGAAGVHVMGSYRPLVNSVVRRCDSNAPQQSGVTSQFHIISTASTHSNDLSSDSRDGDLPPAGAHIPAQPNMLFSTEDIVRAAERTRMYDKALSYLENRLLAMLNLYRYTKMPREVIHKTVWPLAWLYSQREMQDSVVGLFRAIRYEGGEDQAGFGYELLCWWSKAQNTYAKSITQQAMRDMTVPPHILEGYVRTLTLCGEWSRAYDVAMQVCSRPEAYVSSTVARCGATAAWLLGRWDDVQHLAERLSMSEQHTTALRLFFLNGVALKSAITEHSSAAYENLRHMITQSKLVIDESLRTLLPLSYTHAYESLTMLQHFTEMEETIDYCHCRSTKGRQQIYERWNNRFRSLNPDSLQPSLLSLMLHSLVLSTGEMADMILHFCETRQSSHPQLAEWAMSWLRHGSFSGRSSNLCSPSAASPASPGPVEGITVATLSSVDKNPKVAVGFITHLWSQGKRQAAVESMEMFLRDCGKDLEENNATCYGDAQLRLGTWKQELHADSFWERGFRDEELGHFHKAIRAVPSSYEAWHSWGLMNYRIQQRDHNLSPEDQRTFVEAAHQGFVGAICRCKDSSEALPAVMRLLQLWVIHNGVELLKETVADSISRIPIDHWVQTIPQLIGHLSSDSHDIREVIGMILRSLCEVHPQATVFPLLVVMMSDASTSGSNHNALNSNGDESNIVSGASAMSDSSNPLARKQEIVQGIIQHCPKRIFHEAEAVAKLLVDVSAIPIEKIRENLSQVAAAWNPNAEYEEDPEEVYRRLQNTLDIFHANRRHLLFTVGDIGQFVQMVMEDDRNGQREKAAGVVSQLIEEISKHVAEKLGREPQKAMEPLLHLRNLSVAVFGEYDGHCRDHYPTIASFSSKLDVIPSKKRPRRIQLNGSDGCLYTYCLKGNEDIRMDERVMQLLGMVNVLLSHTRISSSAYIHRFPVIPISSNVGLLGWVENANTINNTICNYRTNISNVRTHQESSVLRAYVGSFGNWDKLSLIQRTEILDFVMQSEHCEAVDVSRAMWHRANTAEQWLDRRTAFTVSLATMSMVGYVLGLGDRHLGNILISMSSGKIVHIDFGDSFDVGRLRHVLPETIPFRLTRMLTNAMEVFGVDGVFRASATRTQTTLHKNRDSIMALLSAFVHDPIVQYKGKMKNIMEKSRSPQDIAERIRNKLRGMEMAIDHSKVNIFNTTQDSCRRPDLLYMSTAFDDTAVRTQSLAMTPEEQVSFLIDEATRIDNYTTMYFGWGPLW

Target ORF information:

RefSeq Version	XM_001465208.2
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative phosphatidylinositol 3-kinase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001465208.2

ORF Insert Sequence:

ATGCAGCAGA TGCACTGGAC CGCGGCGCGC GGCCAGCTGT CTGGTCCTCA ACACTCGCAA 
CACCCTTCAC CGCAGGCATC ATCCACCCAC GCTAGTCAGA AGCAGCAGCA GCAGCAGCAC 
TTGCATCAGA ATGGGCCTCC GGGGCTGGCC GCGGCCGGCA TGGGCGGCGG CAGTGCAGGG 
GTGAATATTT CTTCAGGTCC CGCCGCCGGC GCGTCTGCTG TTCGCGCCAA CGTCAACAGC 
AACGCCTTTC ATAGCCCCCG CTTTTCGCAC CCCATGGGCC CGCTACCGCC CGCTCAGAAT 
CCGCAGCAGC AGGTCTTTTC CGTTTCACAG CAGCCTCCTG GTGCGCAGCT GCAGCAGCAA 
CAGCGCTCCC CTCGATCACT GTCAGGTCCG ACTCCCAATA ACGTCGTGAG TGGTAATAGA 
AGTGGCCTCG AGACGAGCGC GGCGCTCGCC GGGCGCGAGG CGCCGGCCAT CTCAGGCGCC 
AACTCCGCCG CCGCCCACAC AAGCTCTGCC GCAGTCGGAG TGTTCTTAAG CGGTACGCGC 
ACGAACTTCA CCGACTTCGG AGCGGCAGCA GCGTACGGAC TCGGTGCCGG TGGCGTTGGC 
TCGCCGAGCG GGCCCACCGC CAACGCCGGC AGCATCCTCG CAGATGCAAA TGATGAGGAG 
GTGAACCAGC TGTTACGGGA GCTAGAGTTC TCAGTGGACA GCCCTCACGC GTTGGCAGTG 
CTGCGAGACG CCGTGGACGC CATCGAAGCA CGGGCGCTGG CCAACTTTGA CCCTCTGTCG 
CGAGACACGT TCCTGGAGCA GCGCATCCGC GATGTCCTGA CGGAGCTGTC GCGAACGTCG 
AACGAGCGCT CCCTGCTGCT CGCCGTGAGT CTCATAGAGG CGTTGATCCC GATCGCCTAC 
ACCAGTCCTC ACTCCAAGTA CACACGCCTG TGCCGCAGTC TCTCGGTGCT GCGGCAGAGC 
GGGAATGAGA AGGCCGCCAG GGAGGCACAG CGGGTGTACA AGCTGCTGCT GGAGGCGGAT 
GCGCGCTACC CAGGGCCGGA GTCGCCGCTG AAGGCGTTTG TGCAGAAGGA GCTCCGCGAA 
AATTGCGAGC AGGCGCTCCT GCAACTCCAC GGTCGCGTGC AGCAGGCGAA AGCGGCCTTT 
CCGGCGCAGT TCTTTTCCGT CATGTTGGGC TGCGCCGCCA CTGCGGAAGT GAACCCGCGC 
TACATCTCTG GCAAAACTCG TGCGCAGGCG ATTCAGCTCG GCACGATGCT CACAGGCAGC 
CCCGATCCGC TGCTCCGCAA GGCTGCCTAC GAGTGCCTCG TCGCCATCTT TCGAAACACG 
AGCAACATGC GACCCAACGA GCTGGTTCTG GAGAGCCGCC GCCTCTGCGA TGAGTGCATG 
CGAACACTGA ACGCTAGTCA GCACAACGAG GGCCACGTTG TATCGGCTCT GAAGGCGCTG 
CACGCCCTCC TCAGCAGTCG CGGGCTCTCG GCGATCGACA GACAGGTGAT ACCGCAGCTG 
TGCGTGCTCG TCACGGAGCA GCACCGGGTG TCCAAGTCGC TACTGGTGCG CAGCGCAGTG 
TGCGACCTTG TGCCCATCAT TGCGCCGGTC GACATTGCGT CTATGCCGCG CCGCACCACG 
TACTGCGCTA TCATCATGGA GCCAGTGAAG AACGTGCGCG ATGAGCGCTT CAAGGCGCAC 
GAGCTGCATA ATGTGGCTTC CTTTGTCCGC AGTGTCGGGT ATCGCGTGCT TGACGCTACA 
AATCGCACAA ACTTGGAGTC GATCCTGCAC CGCTACATCT CCCGTCCGGA GACGCAGGAG 
GCTTGCTGGT CCATCATGGC TGCCATCTGC GCATCCTCGG CGTCGGCCAG CGGAGGCGCG 
GCATCGGTGG CGCCCATGGG TGGGCGCCGC GTAGACTACG TCAGTACATC TACCACACTC 
CCGAGCCCCA CAACGCAGAA CACCCATCAG GACCTCTCCT TGACGCCCTC CATGGTTGAG 
GGGCGCAAGG CCTCGCAGCA CTCCAGCCGC GAAAACTTAG CAACCTCGCC GTCGGCGCTT 
CAGCGGCACT TCTCGTACCA CCACGGTGAC GACAACGACG ATCAGGTCAG CGTTCCACTT 
GTCTCCGTTA GGACCACCTC ACAACGGTCG GCAGGCACGT GCTCCCTCAG CGCCTCCCCT 
ACCTCGACGA ACGCACCAGC CTTCAACCTC TCCACCTTCA GCGTACACCA CACTGTGACC 
ATCGACGACG TTGTCCTGCG CTGCCTGCCG TATCTCGCCC ACGCCATCCT GACCCAGTCA 
CTAGTAGACT CCCTTCGTGT AATACAAGCC TCGTGCCCGC AGTTGCGCGC TGATGTGCAG 
ATGGCTCTGG ACAAGCTGGT GGATAGCGTG CTGAAGGAGC CTTTCGCGCC GTCCGCGACG 
GCGGCGGCTG TGACACTGCC CCAGTATTTT TCCCAGCTGC CAAGCTCCAC GGCGCAGCAG 
CAGCAGCAAG CCCACTCCCT TCATAGCCGT GACGGTGGCG ATGGGAGCGC CGGTGGCTTT 
TCGAGCGCAG CAATGAACAG TGCCTCCCGC ACCGCTGGGT CTCACCCGCT CAGCCCCGCT 
AGTGGCCCGG AGCCGGTCAT GACGCTATCT CGTCCGGGGA CATGCAGCAC GCCGATCGGT 
GTCGGGGCGG TGGGGCGGCT CACACAGTAC TTTCGGCCGA GCGAGAGCGC GGCAATTCAC 
CGAGGCGAGG GCAGCACGAG CAGCCCGACC CCGACCTCCC CGGTGGGAAT GCGCAACACC 
TCTGGCCCTT TACCCTCGGG GAACAACAGC TTCCTGGGCC CCGACAGCGC CTACACCGTC 
GGTGGCGGCG ACTCGAACAG CGGAGCTCCG TACGGTGCTG GCGGACCACT TCGCTGCTCC 
CCGCTCTCCA CGTCGATGAG CGCGCCCTTC AGTCCCGTGA CGCACATGGC GGCAGGTGGC 
AGCGGTGCAG GTGGTGGAAA CGCCGCAGCA GCCCCGCCGT CGGTGCTCAA GAACGACTTG 
ACGGTGGCGC TGAAGGTGTT CACGGAGCGG CCCCTAACGT CGGTGCAGCA CCTGCATGAT 
CTTAAGAACT CGGTGATCGC CTTCCAGCGT CACGAGGATC CGCTCGTGCG GCAGCAGAGC 
AGCGAGACGA TCATTTCGAG CTTGCGGCAG TGGATAGCCT ACGCAAAGGA GCACAAAACC 
TCAACCTACT CCACACGCGT GACAGAGATA TTGGAGGCGT ACATGAAGAA CGTCCTGATG 
GAGGTCGACC GCCAGTGCGG GTTGTACGAG ATTACGCTGC TGGCAAACGC CACGAACCTG 
CGCGCCTTTT TATCAGAGCA GCGCATCCTA TCCTCGCTGC TGAGTTTCCT GAACAGCACA 
GCGCTGGTAC GCGAGAAAAC GGTGGAGCTT CTCGTCGCCA TCACGCAGGA CTCCAAGCCC 
TCCCCCTCTG TCGTGGCGGT GCAGCAGAGC CTGTACGTCA CCGTCGAATC GTGTGTGGTG 
GTGCTCGAGT ACACGAACGA CGTGAGCATG CTCCTGCGCA GCATGGCCGA CCTGCAGACC 
TTTACCCGGC TGTGCATCCG TCAGCTTAAC AACCACCTCA GCCGCATCTT CTACGCCTTT 
CGCAAGCGTC TTTTAGAGGT CAGCGTGCCT GACGTGGTGC TGCTTTCCAT TCTCAAGACA 
CTTGAGATCA TCCTGCAGGC ACTGGCGAAA GACGAAAAGA CCATCTTGCA GTACCAGGAC 
GACGTGCTAG AGCTCTACGA CCCGGTTGTG GAGATACTGA AGCAGTCGCC GTCGCCGACG 
CTGAGCCATG CTGCCATCAA CGTTCTCGTG TACGTACACG AAATGTGCAT CCCGAGCGCC 
TCGACGGATC TGCGTAAGCA GCAGGAGCTG ATCACCGCGC TGACACACGT CTACACCAGC 
AGCACCTGCA CTGTGAACGA ATCTCTGAGT ATGCTGACGC TGTTTGGCCA GCTTGGCGCC 
ATCGACCCCT CCGCACAGTC CGTTGCGACA ACCGCAAAGA AGAAGGAGGA GGCCGTCATC 
CAGGATGAGG CTGACCTGGA GCTCACCTAC GACTACACGG TGATTGTGTA CCGCACGCTG 
AGTCGCATGC TGGACGCGTC ACTCTCCGAT GCCGTCTGCT CTCAGGCTCT GCGCACCCTG 
CTCCAGTTCA TCCGCTTCAC TCAAGACAAG AAGGACCTCG TCGGCGGCGT CCACGCCGTT 
CGGGCGGTGC TGCAGATCAC AAAGCGCGTG ACGGACAGCC CGGCCCTGCG GGTGGAGGCG 
CTGCATGTAT TGGCGGCCGT TACCGCCATG CGGCATGAGC GTATTGTACG GCTCATGTTG 
CCTGAAATCG TGGTCCTGCT GGAGCAGCTG TGGACCCCGA AAGACCGCCT CCTCTTCCGC 
GCTGTCCTGG AGGTGGTGAG CGCGCTCAAG CCCGGCAAGC TGTCCGGCAA GGAGCAGTCG 
GAGTCGTGGG CGTGGCTGTA TCCGCGGCTG GTGGACGTGG CGCTGCAGGA CCGCACTGAG 
TCGCGCGAGT TCTGCCTCCG CGTTGTGGAG ATTGTGCTGA ATGCCAGCTA CATTCCGCCG 
CACTGCATCC CCGTTGTGTT TCCGATGCTA CTTCAGTTCC TGCAGCAATC AGACCAGCTG 
GTGGATGTGC GCAGCCAGTC CCTGTGCGCC GCCGTGCACA TCGTGTGCGA GCTGAAGGCG 
GTGCAGTTCT TTTCGTCGCT CCTGCACGGC ATCTGCACCC TCACGCGGCA CTGCGAGCTG 
AACGAAGATC TCGGCCCGCG CATCTCCACC CCAGGGGTGC GGGAGTCACT CAAGGTCTTG 
GCGGCACTGC ACCCCAGCGG CCGCAGCACG ATCAAGATGT TGCGTGACCG CATCGGCGAG 
GGGGATGAGC GCATGACGAA CCCGACCGGG TCCTCCATAT TCAACACCAT GGGCCTGCCA 
GCGACTAGTG TCACGAGTCT CGCCGGCGGC GGCTCCACCT CCTCGATGCT TGTCATGGGG 
CCAGCGACTG GTAACGCCAG CGGCGGGCAG GGTGGCGGAC TGGGAGTAGA AAATCCAGCG 
AATTCAGGGA TTGGTCCGCT AGTCAATATC GGCGGCTTTG GCGCCTCTAC CGCTGGTGGC 
GGCGCAGCAG CAGCGGGCGG GGCGGCTGGT GGTGGGTCGT ACCTGCGGCT CACAAACGTG 
TCTGAGACCT TTGCAGGGGC TGAGGAAGAG GACGTAGGGC TGACCCTCAG CGCAGACACG 
GCAGGCCAGG AAGTCGACCT CTTCATGAAG CATGTGGAAC TTGGCTTCCG CCTCAAGGAG 
AACAAGTTGC GCGAGTGGTT CGCCGAGTTC CAGAAGTACA TCATTCTCAT CTCCCCACAC 
CCGGCGTTCC GCATGATGGT GGACCTGCTT GACAAGCATG AACCCCTTCG CCGCGGGCTC 
TTCTTGCCTT CGTTCAAGTG CCTCTACGAG TCCCTGTCGG CTGACCAAAT CAAGAAGCTG 
AACGAGATGC TGACCCTCGT GCTGAACTGT TCTGATCACG AGCTGTCGAG CAAGTGTCTC 
GGGCTTGCTG ACTACCTCGA CCATAACGAG CCGAACGTGA AGCCAGAGGT GCTGGAGGTG 
GTGCGGTCGA TGCGGCGAGT CGACTATCAT CGCGTCAGTC ACCCCTCCGA GAACGGGTCG 
CTTCTTCTGC GCAGCACGGA GAGTAGCACG AACAACGTCG TCTCTCTGCG CACCCGCAGC 
TCCGCGTCAG CAGGGCTTCC GAAGGCGGAG TTCTCTACTT CCAGTGACGC TCCCATCGAT 
GTGCTGCCCA TGGCAAAGGT GCGCGGACGG AAGGCGCCGG TTTTCCAGCG CAGCTCAAAC 
AGCAGTGCCG GGGTAGCAGG CGATGAGTCC GGGACGGCAG GTGCCGAAGC CAGCAGTGGT 
GCCTCAACGG CACTTGCCAG CCCCAAAAAT AGCGCAAACG GTCGCCGCAG CGCTTCTCGC 
CGCGATGTGG AGATGTACGA GTTCACCTCC TTTGACGGCA ACGAGAATGG CGCCACCGAG 
TCTGGACGGC AGCAGTCGCA GCCCATCGCC TTGCCGACGT CGGAGACACC GGCCCAGGAA 
GAGGAGGCGG AGCGGCGCAG CTGCTCGCCC GTGGACACGC TCGATGGGGC AGAGAGCGAG 
AACAAGGCGG AGGACGTGGT GGCCCCCGTC AGCACCGACC ATCCGTCGGT TGGAGTGCTA 
GGCGCGGCGG GAGTGCATGT CATGGGAAGC TACAGGCCCC TTGTGAACTC CGTCGTACGT 
CGTTGCGACT CGAACGCGCC GCAGCAGTCC GGGGTGACCA GCCAGTTTCA CATCATCAGC 
ACCGCTAGCA CTCACAGTAA CGACCTCAGC AGCGACAGCC GTGACGGTGA TCTGCCGCCG 
GCCGGGGCCC ACATCCCGGC GCAGCCAAAC ATGCTCTTCT CGACGGAGGA CATCGTCCGC 
GCTGCAGAGA GAACCCGTAT GTACGACAAG GCACTCAGCT ATCTGGAGAA CCGTCTTCTG 
GCCATGCTCA ACCTCTACCG GTACACGAAG ATGCCACGAG AGGTGATCCA CAAGACGGTG 
TGGCCGCTGG CATGGCTCTA CAGCCAGCGT GAAATGCAGG ACTCCGTCGT AGGACTCTTC 
CGCGCTATCC GCTACGAAGG CGGCGAGGAC CAAGCAGGCT TCGGCTACGA GCTGCTCTGC 
TGGTGGAGTA AGGCGCAGAA CACGTACGCC AAGTCCATCA CGCAGCAGGC GATGCGCGAT 
ATGACGGTGC CGCCGCACAT TCTTGAAGGC TACGTGCGCA CCCTCACCCT GTGCGGTGAG 
TGGAGCCGGG CCTATGACGT GGCCATGCAG GTCTGTTCAC GGCCGGAGGC GTACGTTTCC 
TCGACGGTCG CGCGCTGCGG TGCAACGGCC GCGTGGCTGC TCGGGCGGTG GGATGACGTG 
CAACACCTCG CGGAGCGGCT CTCCATGTCG GAGCAGCACA CCACGGCGCT CCGTCTCTTC 
TTTCTCAACG GCGTGGCGCT GAAGAGCGCC ATTACGGAGC ACAGCAGCGC CGCCTACGAG 
AACCTGCGAC ACATGATCAC ACAGTCGAAG CTCGTGATTG ACGAGAGCCT GCGCACTCTT 
CTCCCGCTCA GCTACACGCA TGCCTACGAG AGTCTGACAA TGCTGCAGCA CTTCACGGAG 
ATGGAGGAGA CTATCGACTA CTGCCACTGC CGCTCTACCA AGGGTCGCCA GCAGATCTAC 
GAGCGGTGGA ACAACCGGTT CCGCTCTCTG AATCCGGATT CCCTGCAGCC AAGCCTGCTC 
TCGCTGATGC TGCACTCGCT CGTGCTCAGC ACAGGCGAGA TGGCGGACAT GATTCTGCAC 
TTCTGTGAGA CGCGGCAGTC GAGCCACCCG CAGCTGGCGG AGTGGGCCAT GAGCTGGCTT 
CGACACGGCT CCTTCAGCGG GCGCAGCAGC AACCTCTGTA GCCCGAGTGC GGCATCGCCG 
GCGTCACCGG GGCCCGTGGA GGGCATCACG GTGGCGACGC TGTCATCGGT GGACAAGAAT 
CCAAAGGTGG CTGTCGGCTT CATCACGCAT CTGTGGAGCC AAGGAAAGCG GCAGGCCGCT 
GTTGAGTCCA TGGAGATGTT TCTGCGCGAC TGCGGCAAGG ACCTGGAGGA GAACAACGCC 
ACCTGCTACG GCGACGCGCA GCTGCGGCTC GGCACGTGGA AACAGGAGCT GCACGCCGAC 
TCCTTCTGGG AGCGCGGCTT CCGGGACGAG GAGCTGGGCC ATTTTCACAA GGCTATCCGT 
GCCGTCCCGT CGAGCTACGA GGCGTGGCAC AGCTGGGGCC TCATGAACTA CCGTATCCAG 
CAGCGCGACC ACAACCTCTC CCCCGAGGAC CAGCGCACCT TTGTGGAGGC GGCGCATCAG 
GGCTTTGTTG GCGCCATATG CCGCTGCAAA GACTCCTCCG AGGCGCTGCC GGCCGTCATG 
CGTCTGCTGC AGCTGTGGGT CATCCACAAC GGAGTGGAGC TGCTGAAGGA GACGGTGGCC 
GACAGTATCT CGCGCATCCC GATCGACCAC TGGGTGCAGA CCATTCCGCA GCTGATCGGC 
CACCTCAGCA GCGACAGCCA CGACATCCGC GAAGTCATTG GCATGATCCT GCGCTCGCTC 
TGCGAGGTGC ACCCACAGGC CACCGTCTTT CCGCTCCTCG TCGTGATGAT GTCGGACGCC 
AGCACCAGCG GCAGCAATCA CAACGCCCTC AACAGCAACG GAGATGAGAG TAATATAGTG 
TCGGGTGCGT CCGCCATGTC CGATAGCTCG AATCCGCTGG CGCGGAAGCA GGAGATTGTG 
CAAGGCATCA TCCAGCACTG CCCGAAGCGC ATCTTCCACG AGGCCGAGGC GGTGGCAAAG 
CTGCTTGTGG ACGTGTCCGC CATTCCCATC GAGAAGATTC GCGAGAACCT GAGCCAGGTC 
GCTGCTGCGT GGAACCCAAA CGCCGAGTAC GAGGAGGACC CGGAGGAAGT GTACCGCCGC 
CTGCAGAACA CACTCGACAT CTTCCACGCC AACCGGCGCC ACCTTCTCTT CACCGTCGGT 
GACATTGGTC AGTTTGTGCA GATGGTCATG GAGGACGACC GCAACGGACA GCGAGAGAAG 
GCGGCGGGCG TCGTCAGCCA ACTCATCGAA GAGATCTCGA AGCATGTGGC GGAGAAGCTG 
GGCCGTGAGC CTCAGAAGGC GATGGAGCCG CTGCTGCATC TGCGTAATCT GTCCGTCGCG 
GTATTCGGGG AGTACGACGG TCACTGCCGC GACCATTACC CGACTATCGC ATCCTTTAGC 
TCCAAGCTGG ACGTCATCCC GTCGAAGAAG CGGCCGCGCC GCATCCAGCT CAACGGCTCG 
GACGGCTGTC TCTACACATA CTGCCTAAAG GGCAACGAGG ACATTCGCAT GGACGAGCGT 
GTCATGCAGC TGCTTGGAAT GGTGAACGTC CTACTGAGCC ACACCCGTAT CTCAAGCAGC 
GCCTACATCC ACCGCTTCCC TGTCATCCCG ATCAGCTCCA ACGTGGGGCT TCTTGGCTGG 
GTGGAGAACG CGAATACAAT CAACAACACC ATCTGCAACT ACCGTACCAA CATCTCGAAC 
GTGCGCACGC ATCAGGAGTC GAGTGTGCTG CGCGCCTACG TAGGGTCGTT CGGCAACTGG 
GACAAGCTCA GCCTCATTCA GCGCACGGAG ATACTCGACT TTGTGATGCA GAGCGAGCAC 
TGCGAAGCCG TTGACGTTTC TCGAGCAATG TGGCACCGCG CCAACACGGC GGAGCAGTGG 
CTTGACCGCC GAACTGCCTT TACAGTATCC TTGGCGACCA TGTCCATGGT GGGCTACGTG 
CTCGGCCTCG GCGATCGGCA TCTCGGCAAC ATCCTCATCT CTATGTCCTC TGGCAAGATT 
GTGCACATTG ACTTTGGAGA CAGCTTCGAT GTCGGTCGGC TACGCCACGT TCTGCCGGAG 
ACGATCCCGT TCCGCCTCAC GCGCATGCTG ACGAATGCGA TGGAGGTCTT CGGCGTGGAC 
GGCGTCTTCC GCGCCTCCGC AACGCGGACG CAGACGACGC TGCACAAGAA CCGTGATAGC 
ATCATGGCCC TGCTCTCCGC CTTCGTCCAC GACCCGATTG TGCAGTACAA GGGCAAGATG 
AAGAACATCA TGGAGAAGAG CCGGTCGCCG CAGGACATTG CAGAGCGCAT TCGTAACAAG 
CTGCGCGGGA TGGAGATGGC GATTGACCAC AGCAAGGTGA ACATCTTTAA CACGACGCAA 
GACAGCTGCC GCCGGCCAGA CCTCCTGTAC ATGTCGACCG CCTTCGATGA TACTGCCGTG 
CGGACACAGT CGTTGGCGAT GACGCCGGAG GAGCAGGTTA GCTTCTTGAT TGACGAGGCG 
ACGCGCATCG ACAACTACAC GACGATGTAC TTCGGCTGGG GCCCGCTGTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_20_1140 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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