GP63-4 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	GP63-4
Entrez Gene ID	5067075
Full Name	GP63, leishmanolysin
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001463660.2	XP_001463697.2	GP63, leishmanolysin

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GP63-4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GP63-4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc01005	XM_001463660.2 Latest version!	Leishmania infantum JPCM5 GP63, leishmanolysin (GP63-4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GP63-4 ( XM_001463660.2 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001463697.2 Leishmania infantum JPCM5 GP63, leishmanolysin (GP63-4), partial mRNA.

Price & Availability↑

CloneID	OLc01005
Clone ID Related Accession (Same CDS sequence)	XM_001463660.2
Accession Version	XM_001463660.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1797bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	GP63, leishmanolysin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009394). On Jul 14, 2011 this sequence version replaced XM_001463660.1. COMPLETENESS: incomplete on both ends.

ATGTCCGTCG ACAGCAGCAG CACGCACCGG CACCGCAGCG TCGCCGCGCG CCTGGTGCGC 
CTCGCGGCTG CCGGCGCCGC AGTCATCGCT GCTGTCGGCA CCGCGGCCGC GTGGGCACAC 
GCCGGTGCGG TGCAGCACCG CTGCATCCAC GACGCGATGC AGGCACGCGT GCGGCAGTCG 
GTGGCGCGCC ACCACACGGC CCCCGGCGCC GTGTCCGCGG TGGGCCTGCC GTACGTTACT 
CTCGACACCG CGGCCGCCGC CGATCGCCGG CCGGGCAGCG CGCCCACAGT CGTGCGCGCC 
GCGAACTGGG GCGCGCTGCG CATCGCCGTC TCCACCGAGG ACCTCACCGA CCCCGCCTAC 
CACTGCGCTC GCGTCGGGCA GCACATCAAG AGGCGACTTG GCGGCGTCGA CATATGCACC 
GCCGAGGACA TCCTCACCGA CGAGAAGCGC GACATCCTGG TCAAGCACCT CATCCCGCAG 
GCGCTGCAGC TGCACACGGA GCGGCTGAAG GTGCGGCAGG TGCAGGACAA GTGGAAGGTG 
ACGGGCATGG GCGACGATGT GTGCAGCGAC TTCAAGGTGC CGCCGGCGCA CATCACCGAT 
GGCCTGAGCA ACACCGACTT CGTGATGTAC GTCGCCTCCG TGCCGAGCGA GGGGGATGTG 
CTGGCGTGGG CCGCGACCTG CCAGGTGTTC TCTGACGGCC ATCCAGCCGT GGGCGTCATC 
AACATCCCCG CGGCGAACAT TGCGTCGCGG TACGACCAGC TGGTGACGCG TGTCGTCACG 
CACGAGATGG CGCACGCGCT CGGCTTCAGC GACACATTCT TTACAGACAA GCGCATGCTG 
CACAATGTGG GGAAAATACG AGGAAAGCCG CATAACGCTC CCGTGATCAA CAGCAGCACG 
GCGGTGGCGA AGGCGCGCGA GCAGTACGGC TGCGACACCT TGGAGTATCT GGAGATGGAG 
GATGAGGGCG GTGCGGTCTC CGCCGGGTCG CACATCAAGA TGCGCAACGC GCAGGACGAG 
CTCATGGCGC CTGCCGCAGC TGCCGGGTAC TACAGCGCCC TGACCATGGC CATCTTCCAG 
GACCTCGGCT TCTACCAGGC GGACTTCAGC AAGGCCGAGG TGATGCCGTG GGGCCGGAAC 
GCCGGCTGCG CCTTCCTCAG CGAGAAGTGC ATGGAGGACG GCATCACGAA GTGGCCAGCG 
ATGTTCTGCA ACAGCGATGA CGCCCTGCGC TGCCCCACCA GTCGTCTCAG CCTCGGAGCG 
TGCTCTTTGG CCACCTTCCA AAGTCTTCCG CCGTACTGGC AGTACTTCAC GGACCCGTCC 
CTCGCCGGCA TCTCCGCCTT CATGGACTAC TGCCCTGTCG TGGTGCCCTT CGGCAACGGC 
AGCTGCGCAC AGAATGCCTC TAAGGTGATG GCAGCTGTGC AGGCATTTAA CGTCTTCTCC 
GACGCGGCGC GCTGCATCGA TGGCGCCTTC AGGCCGAAGA CGACCGAAAC CGTAACAAAT 
TCGTACGCCG GACTGTGCGC CAACGTGCGG TGCGACACGG CCACGCGCAC GTACAGCGTG 
CAGGTGCGCG GCGGCAGCGG CTACGCCAGT TGCACGCCGG GCCTCAGAGT TGAGCTGAGC 
ACCGTGAGCA GCGCCTTCGA GGAGGGCGGC TACATCACGT GCCCGCCGTA CGTGGAGGTG 
TGCCAGGGCA ACGTGCAGGC TGCCAAGGAC GGCGGCAACG CCGCGGCTGG TCGCCGTGGT 
CCGCGCGCCG CGGCGACGGC GCTGCTGGTG GCCGCGCTGC TGGCCGTGGC GCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001463697.2
CDS	1..1797
Translation	MSVDSSSTHRHRSVAARLVRLAAAGAAVIAAVGTAAAWAHAGAVQHRCIHDAMQARVRQSVARHHTAPGAVSAVGLPYVTLDTAAAADRRPGSAPTVVRAANWGALRIAVSTEDLTDPAYHCARVGQHIKRRLGGVDICTAEDILTDEKRDILVKHLIPQALQLHTERLKVRQVQDKWKVTGMGDDVCSDFKVPPAHITDGLSNTDFVMYVASVPSEGDVLAWAATCQVFSDGHPAVGVINIPAANIASRYDQLVTRVVTHEMAHALGFSDTFFTDKRMLHNVGKIRGKPHNAPVINSSTAVAKAREQYGCDTLEYLEMEDEGGAVSAGSHIKMRNAQDELMAPAAAAGYYSALTMAIFQDLGFYQADFSKAEVMPWGRNAGCAFLSEKCMEDGITKWPAMFCNSDDALRCPTSRLSLGACSLATFQSLPPYWQYFTDPSLAGISAFMDYCPVVVPFGNGSCAQNASKVMAAVQAFNVFSDAARCIDGAFRPKTTETVTNSYAGLCANVRCDTATRTYSVQVRGGSGYASCTPGLRVELSTVSSAFEEGGYITCPPYVEVCQGNVQAAKDGGNAAAGRRGPRAAATALLVAALLAVAL

Target ORF information:

RefSeq Version	XM_001463660.2
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 GP63, leishmanolysin (GP63-4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001463660.2

ORF Insert Sequence:

ATGTCCGTCG ACAGCAGCAG CACGCACCGG CACCGCAGCG TCGCCGCGCG CCTGGTGCGC 
CTCGCGGCTG CCGGCGCCGC AGTCATCGCT GCTGTCGGCA CCGCGGCCGC GTGGGCACAC 
GCCGGTGCGG TGCAGCACCG CTGCATCCAC GACGCGATGC AGGCACGCGT GCGGCAGTCG 
GTGGCGCGCC ACCACACGGC CCCCGGCGCC GTGTCCGCGG TGGGCCTGCC GTACGTTACT 
CTCGACACCG CGGCCGCCGC CGATCGCCGG CCGGGCAGCG CGCCCACAGT CGTGCGCGCC 
GCGAACTGGG GCGCGCTGCG CATCGCCGTC TCCACCGAGG ACCTCACCGA CCCCGCCTAC 
CACTGCGCTC GCGTCGGGCA GCACATCAAG AGGCGACTTG GCGGCGTCGA CATATGCACC 
GCCGAGGACA TCCTCACCGA CGAGAAGCGC GACATCCTGG TCAAGCACCT CATCCCGCAG 
GCGCTGCAGC TGCACACGGA GCGGCTGAAG GTGCGGCAGG TGCAGGACAA GTGGAAGGTG 
ACGGGCATGG GCGACGATGT GTGCAGCGAC TTCAAGGTGC CGCCGGCGCA CATCACCGAT 
GGCCTGAGCA ACACCGACTT CGTGATGTAC GTCGCCTCCG TGCCGAGCGA GGGGGATGTG 
CTGGCGTGGG CCGCGACCTG CCAGGTGTTC TCTGACGGCC ATCCAGCCGT GGGCGTCATC 
AACATCCCCG CGGCGAACAT TGCGTCGCGG TACGACCAGC TGGTGACGCG TGTCGTCACG 
CACGAGATGG CGCACGCGCT CGGCTTCAGC GACACATTCT TTACAGACAA GCGCATGCTG 
CACAATGTGG GGAAAATACG AGGAAAGCCG CATAACGCTC CCGTGATCAA CAGCAGCACG 
GCGGTGGCGA AGGCGCGCGA GCAGTACGGC TGCGACACCT TGGAGTATCT GGAGATGGAG 
GATGAGGGCG GTGCGGTCTC CGCCGGGTCG CACATCAAGA TGCGCAACGC GCAGGACGAG 
CTCATGGCGC CTGCCGCAGC TGCCGGGTAC TACAGCGCCC TGACCATGGC CATCTTCCAG 
GACCTCGGCT TCTACCAGGC GGACTTCAGC AAGGCCGAGG TGATGCCGTG GGGCCGGAAC 
GCCGGCTGCG CCTTCCTCAG CGAGAAGTGC ATGGAGGACG GCATCACGAA GTGGCCAGCG 
ATGTTCTGCA ACAGCGATGA CGCCCTGCGC TGCCCCACCA GTCGTCTCAG CCTCGGAGCG 
TGCTCTTTGG CCACCTTCCA AAGTCTTCCG CCGTACTGGC AGTACTTCAC GGACCCGTCC 
CTCGCCGGCA TCTCCGCCTT CATGGACTAC TGCCCTGTCG TGGTGCCCTT CGGCAACGGC 
AGCTGCGCAC AGAATGCCTC TAAGGTGATG GCAGCTGTGC AGGCATTTAA CGTCTTCTCC 
GACGCGGCGC GCTGCATCGA TGGCGCCTTC AGGCCGAAGA CGACCGAAAC CGTAACAAAT 
TCGTACGCCG GACTGTGCGC CAACGTGCGG TGCGACACGG CCACGCGCAC GTACAGCGTG 
CAGGTGCGCG GCGGCAGCGG CTACGCCAGT TGCACGCCGG GCCTCAGAGT TGAGCTGAGC 
ACCGTGAGCA GCGCCTTCGA GGAGGGCGGC TACATCACGT GCCCGCCGTA CGTGGAGGTG 
TGCCAGGGCA ACGTGCAGGC TGCCAAGGAC GGCGGCAACG CCGCGGCTGG TCGCCGTGGT 
CCGCGCGCCG CGGCGACGGC GCTGCTGGTG GCCGCGCTGC TGGCCGTGGC GCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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GP63-4 cDNA ORF clone, Leishmania infantum JPCM5

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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