LINJ_09_0640 cDNA ORF clone, Leishmania infantum JPCM5

Gene
Clones
gRNAs

Summary

Gene Symbol	LINJ_09_0640
Entrez Gene ID	5066920
Full Name	putative ATP-dependent DNA helicase
Gene Type	protein-coding
Organism	Leishmania infantum JPCM5

mRNA and Protein(s)

mRNA	Protein	Name
XM_001463484.1	XP_001463521.1	putative ATP-dependent DNA helicase

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Rogers MB, Hilley JD, Dickens NJ, Wilkes J, Bates PA, Depledge DP, Harris D, Her Y, Herzyk P, Imamura H, Otto TD, Sanders M, Seeger K, Dujardin JC, Berriman M, Smith DF, Hertz-Fowler C, Mottram JC
Genome research21(12)2129-42(2011 Dec)

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Peacock CS, Seeger K, Harris D, Murphy L, Ruiz JC, Quail MA, Peters N, Adlem E, Tivey A, Aslett M, Kerhornou A, Ivens A, Fraser A, Rajandream MA, Carver T, Norbertczak H, Chillingworth T, Hance Z, Jagels K, Moule S, Ormond D, Rutter S, Squares R, Whitehead S, Rabbinowitsch E, Arrowsmith C, White B, Thurston S, Bringaud F, Baldauf SL, Faulconbridge A, Jeffares D, Depledge DP, Oyola SO, Hilley JD, Brito LO, Tosi LR, Barrell B, Cruz AK, Mottram JC, Smith DF, Berriman M
Nature genetics39(7)839-47(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LINJ_09_0640 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LINJ_09_0640 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLc00875	XM_001463484.1 Latest version!	Leishmania infantum JPCM5 putative ATP-dependent DNA helicase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LINJ_09_0640 ( XM_001463484.1 ) cDNA ORF clone, Leishmania infantum JPCM5 -> XP_001463521.1 Leishmania infantum JPCM5 putative ATP-dependent DNA helicase partial mRNA.

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CloneID	OLc00875
Clone ID Related Accession (Same CDS sequence)	XM_001463484.1
Accession Version	XM_001463484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2991bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1436889600000
Organism	Leishmania infantum JPCM5
Product	putative ATP-dependent DNA helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_009393). COMPLETENESS: incomplete on both ends.

ATGGACGAGT CACAAATACG CGCTGCCACG TACCCGGCGG GCGCCGCGCT GATACTGCAG 
GCTGGCGCCG GCAGCGGAAA GACCCAGACG ATGGCGGCAC GCATCGCCTA CCTCCTCCAG 
AGCGGTGTGC CGGGACACTC CATCCTCGGC ATCTGCTTCA CGCGTCAAGC AGCGGAGACG 
CTGCGGGAGC GGGTGCGGTC TACCCTTCCT CCCACGCTCA CCCGCGAGGC GCACGCCTTG 
AAGCTCAAGA CGTTTCACGC CTTCGGGCTC GAGTGCTTGC GGCGCTTTGG TGCCCTCCAG 
TCCGACACGC ACGTCCTCGA TGCGCGGCAG CAGTACGAAC TTGCCCGCCG TGTTGTCGAC 
ACGTACGCGC AACGTGAAAA GAGCAGCGAG GCCGTGACGG ACCTGGTCGA CTACGTGAAT 
CGGGTAAAGA CGATGAAGGT GCCGCCGATT CCTCAGTCGG ACCCTGCCCT GCAGGATGCA 
TACCTCTTCC CCTACTACCA AAAGGCTCTG CATGAGGAGC GCAACGCCGT GGACTTTGGA 
GACTTGCAGC AGATGTTTTA CGACCTGATC CGACCCGTCC CCGTCACCGC ATTCGCAGCC 
TCGCAAGGGT GCGGCGGGGG GCTGGACGAG CAGCAGCCCT CACAGGGCGA ACACCAACCC 
CAGCAACAGC AGCAACAGGG AAAGATGGTC CCATCCGATG TGTGCACCGC GCTGCGTGCC 
GAGTACACAC ACTTCGTCGT CGACGAGTTC CAGGACTTCA ACGAGATTCA GGTGGAGCTG 
CTGGCGCTGC TGGCCGGCGA CGCGTGTCGC GTGACCTGCG TGGGAGACCC CAATCAGTGC 
ATCTACACGT GGCGCGGCGC CATGCCAAAC GTCTTCGGGG TGTGGAAGAA GCGCTTTCCT 
CAGACAGCAA TGCTGACGCT GGCGATGAAC TACCGCAGCG ACGGGCCCAT TGTGGAAGCG 
GCCAATCGCG TGGTGAAGGC AACGCAGATG GCACACCACC ATCGCGAGGA GCGCGCCGTG 
ACGCTCGTGC AGTGTGCCAG CGAGGATGAT GAGCTGCAGG CGGTGCCACT GGTGATCAAC 
CACGTGCTAC GCCGTCGAGA TGCCCACCTC GGCTACGGTG ACATCGCCAT TCTCTGTCGC 
TCCCGTCGGC GGGTGCAACT CTACTGCGAT GTGCTGCAGT CGCAGCGCAT TCCAGTGCGG 
CAGCTGAAGG GGATGTCGGT TGACCATCTC GCAAGCATGC GCTCGCTGCT TGCGTTTCTG 
CGACTGTGTG TGTCACCGCA TGGCCCGGAA GGTGACGCAA ATGTGCGCAC AGTCCTGAAT 
ACCGCCCCGC TGCATCGGCT ATCGGCCGGG GCGGCCAAGA AATTCTTCCT GTCGCTTGAC 
TCAGTCTGTC AGGCGCGGCG CGCGATGGAA GCGGCGCGGA TACGGTTGTG GCGCCACACC 
ATCCACGTGG ATAACGGCAG CGAGGTTGGC GCAGATAACG GAGGCGCGCA GGGGTCGCGA 
AGTGAGGGCT TACAGCCCGG CGCTGCGTGT ACCTCGGTCA TCAGCGGTGG TTGCCCCGCC 
GTCGCCACGC CTGGAGTCCA CCCCGAGTCG TACTCCTTCT TTGCTGTTTT GCAGGAGCTC 
GTGTACCACA ACTTTTCCCA TGAGCTCTTC CCCAAGCTGG AGGTGTCCAA AAAGAACCAG 
AAGAACATCC GCAGCGTGGT GCGCATCGTG GTGCATGCCA GGGAGCTGCT TGCGCAGCCG 
TCTTGCGACG TCGAGCAAGT CCTTCGATAC GTCCTGCGCG AAGGTGGATA CGAGGGCGAA 
AGCATGGCCG CGGTAGCAGC TCGCACGTCC ACCCCCTCAG CAGCCACGAG GGGGACGAAG 
CGAGCGCGTA ACTCCGCCGC CGGTTGGGGT AGTGACCGCT GTGCAGACGA GGCGTCCGGG 
CGAAGCAGCG CTTCGGCGTA CCAGCCTCGC GTTGGCGCCC TTCTCATGGA GCAATGCGCC 
TCGCAGCACA ATCAACGGCA GCAGCGCTAT ACCTCCTCCT CCGCCGCCGC CTCCTGCAGC 
AGCGCCGGTG GAGGCGGCGG TAGGTGGGAT CGGTGGCGCC ATGGCAGCCG GCGGTCAGGT 
GCCGCATCCG GCAGTGACGG CGTCGTCGAT GAGGACGTTT TGCCCCCCGA GGAGGAGGCG 
GCGGTGTGGC AGGAGCAGCG CATGAACCTG TCCGAGTTGG TGCTGCACAC GTACCACTCC 
GTGCGGGAGG CGCTGGAGCG TGAGGTGGCG CATGAGCTAG AGAAGGAAGG CGGCGGTGGT 
GAGGGTAGAG GGGAGGAGGC GTCGTCGCGG TCTCGCCCGT CCCAGGCTAG CGGCAACCAC 
AGCAACAGTC GAACGCAACT GTTTTCCAAG GCGACTGCTG CCGACGACTC GGTCAGCAAC 
TTCAGTGCCG GGAGCACCAC CACGACTATG ACGGTCATGC AGGCTCTGCG CCCACCCGCC 
GTGGTGCTGC ATCGCGTGCT GGACGAGTTT GTGTCGCTCG TGTCCTCGGA CGACTACGGC 
CCCATGCGGG AGATCGGGAA CGCGGACGAT GCTGCAGGAG CAGCCGCGGG GGCGGGCACC 
GCTGGTAACA GCGGCCGCCT CCCTACCAGT ACCTCGCCAC ACTGGATCGG GCAGGTCACT 
GTCGGCACTG TGCACCGCGC GAAGGGCATG GAGTGGCCGG CGGTGCTGCT GCCCGGGTGC 
TGGGTCGGCG AGTACCCGGT GCGGCCACGC GAAGAAGAAA AACGCGTCTT TTACGTGGGC 
ATGAGTCGAG CCATGAAGCA CCTTGTGTGC TTCACCGCAG CAACGCGCGA GTGCAGGTCT 
GGCAGCGGTC AGGCGGCAAC CACGGCAAGC GTGATCGACC TGCCCGCGCA GCACACACAA 
AGCGGAACGC TGGAGCCGAC GCCGTACCTG GCTGCCCTCG GCGACAAGCT GGAGCGGGTC 
AACTACGCCG ACCTCAAGAC AGCGTATCTG AAGGAGCAAG GGTGCCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001463521.1
CDS	1..2991
Translation	MDESQIRAATYPAGAALILQAGAGSGKTQTMAARIAYLLQSGVPGHSILGICFTRQAAETLRERVRSTLPPTLTREAHALKLKTFHAFGLECLRRFGALQSDTHVLDARQQYELARRVVDTYAQREKSSEAVTDLVDYVNRVKTMKVPPIPQSDPALQDAYLFPYYQKALHEERNAVDFGDLQQMFYDLIRPVPVTAFAASQGCGGGLDEQQPSQGEHQPQQQQQQGKMVPSDVCTALRAEYTHFVVDEFQDFNEIQVELLALLAGDACRVTCVGDPNQCIYTWRGAMPNVFGVWKKRFPQTAMLTLAMNYRSDGPIVEAANRVVKATQMAHHHREERAVTLVQCASEDDELQAVPLVINHVLRRRDAHLGYGDIAILCRSRRRVQLYCDVLQSQRIPVRQLKGMSVDHLASMRSLLAFLRLCVSPHGPEGDANVRTVLNTAPLHRLSAGAAKKFFLSLDSVCQARRAMEAARIRLWRHTIHVDNGSEVGADNGGAQGSRSEGLQPGAACTSVISGGCPAVATPGVHPESYSFFAVLQELVYHNFSHELFPKLEVSKKNQKNIRSVVRIVVHARELLAQPSCDVEQVLRYVLREGGYEGESMAAVAARTSTPSAATRGTKRARNSAAGWGSDRCADEASGRSSASAYQPRVGALLMEQCASQHNQRQQRYTSSSAAASCSSAGGGGGRWDRWRHGSRRSGAASGSDGVVDEDVLPPEEEAAVWQEQRMNLSELVLHTYHSVREALEREVAHELEKEGGGGEGRGEEASSRSRPSQASGNHSNSRTQLFSKATAADDSVSNFSAGSTTTTMTVMQALRPPAVVLHRVLDEFVSLVSSDDYGPMREIGNADDAAGAAAGAGTAGNSGRLPTSTSPHWIGQVTVGTVHRAKGMEWPAVLLPGCWVGEYPVRPREEEKRVFYVGMSRAMKHLVCFTAATRECRSGSGQAATTASVIDLPAQHTQSGTLEPTPYLAALGDKLERVNYADLKTAYLKEQGCL

Target ORF information:

RefSeq Version	XM_001463484.1
Organism	Leishmania infantum JPCM5
Definition	Leishmania infantum JPCM5 putative ATP-dependent DNA helicase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001463484.1

ORF Insert Sequence:

ATGGACGAGT CACAAATACG CGCTGCCACG TACCCGGCGG GCGCCGCGCT GATACTGCAG 
GCTGGCGCCG GCAGCGGAAA GACCCAGACG ATGGCGGCAC GCATCGCCTA CCTCCTCCAG 
AGCGGTGTGC CGGGACACTC CATCCTCGGC ATCTGCTTCA CGCGTCAAGC AGCGGAGACG 
CTGCGGGAGC GGGTGCGGTC TACCCTTCCT CCCACGCTCA CCCGCGAGGC GCACGCCTTG 
AAGCTCAAGA CGTTTCACGC CTTCGGGCTC GAGTGCTTGC GGCGCTTTGG TGCCCTCCAG 
TCCGACACGC ACGTCCTCGA TGCGCGGCAG CAGTACGAAC TTGCCCGCCG TGTTGTCGAC 
ACGTACGCGC AACGTGAAAA GAGCAGCGAG GCCGTGACGG ACCTGGTCGA CTACGTGAAT 
CGGGTAAAGA CGATGAAGGT GCCGCCGATT CCTCAGTCGG ACCCTGCCCT GCAGGATGCA 
TACCTCTTCC CCTACTACCA AAAGGCTCTG CATGAGGAGC GCAACGCCGT GGACTTTGGA 
GACTTGCAGC AGATGTTTTA CGACCTGATC CGACCCGTCC CCGTCACCGC ATTCGCAGCC 
TCGCAAGGGT GCGGCGGGGG GCTGGACGAG CAGCAGCCCT CACAGGGCGA ACACCAACCC 
CAGCAACAGC AGCAACAGGG AAAGATGGTC CCATCCGATG TGTGCACCGC GCTGCGTGCC 
GAGTACACAC ACTTCGTCGT CGACGAGTTC CAGGACTTCA ACGAGATTCA GGTGGAGCTG 
CTGGCGCTGC TGGCCGGCGA CGCGTGTCGC GTGACCTGCG TGGGAGACCC CAATCAGTGC 
ATCTACACGT GGCGCGGCGC CATGCCAAAC GTCTTCGGGG TGTGGAAGAA GCGCTTTCCT 
CAGACAGCAA TGCTGACGCT GGCGATGAAC TACCGCAGCG ACGGGCCCAT TGTGGAAGCG 
GCCAATCGCG TGGTGAAGGC AACGCAGATG GCACACCACC ATCGCGAGGA GCGCGCCGTG 
ACGCTCGTGC AGTGTGCCAG CGAGGATGAT GAGCTGCAGG CGGTGCCACT GGTGATCAAC 
CACGTGCTAC GCCGTCGAGA TGCCCACCTC GGCTACGGTG ACATCGCCAT TCTCTGTCGC 
TCCCGTCGGC GGGTGCAACT CTACTGCGAT GTGCTGCAGT CGCAGCGCAT TCCAGTGCGG 
CAGCTGAAGG GGATGTCGGT TGACCATCTC GCAAGCATGC GCTCGCTGCT TGCGTTTCTG 
CGACTGTGTG TGTCACCGCA TGGCCCGGAA GGTGACGCAA ATGTGCGCAC AGTCCTGAAT 
ACCGCCCCGC TGCATCGGCT ATCGGCCGGG GCGGCCAAGA AATTCTTCCT GTCGCTTGAC 
TCAGTCTGTC AGGCGCGGCG CGCGATGGAA GCGGCGCGGA TACGGTTGTG GCGCCACACC 
ATCCACGTGG ATAACGGCAG CGAGGTTGGC GCAGATAACG GAGGCGCGCA GGGGTCGCGA 
AGTGAGGGCT TACAGCCCGG CGCTGCGTGT ACCTCGGTCA TCAGCGGTGG TTGCCCCGCC 
GTCGCCACGC CTGGAGTCCA CCCCGAGTCG TACTCCTTCT TTGCTGTTTT GCAGGAGCTC 
GTGTACCACA ACTTTTCCCA TGAGCTCTTC CCCAAGCTGG AGGTGTCCAA AAAGAACCAG 
AAGAACATCC GCAGCGTGGT GCGCATCGTG GTGCATGCCA GGGAGCTGCT TGCGCAGCCG 
TCTTGCGACG TCGAGCAAGT CCTTCGATAC GTCCTGCGCG AAGGTGGATA CGAGGGCGAA 
AGCATGGCCG CGGTAGCAGC TCGCACGTCC ACCCCCTCAG CAGCCACGAG GGGGACGAAG 
CGAGCGCGTA ACTCCGCCGC CGGTTGGGGT AGTGACCGCT GTGCAGACGA GGCGTCCGGG 
CGAAGCAGCG CTTCGGCGTA CCAGCCTCGC GTTGGCGCCC TTCTCATGGA GCAATGCGCC 
TCGCAGCACA ATCAACGGCA GCAGCGCTAT ACCTCCTCCT CCGCCGCCGC CTCCTGCAGC 
AGCGCCGGTG GAGGCGGCGG TAGGTGGGAT CGGTGGCGCC ATGGCAGCCG GCGGTCAGGT 
GCCGCATCCG GCAGTGACGG CGTCGTCGAT GAGGACGTTT TGCCCCCCGA GGAGGAGGCG 
GCGGTGTGGC AGGAGCAGCG CATGAACCTG TCCGAGTTGG TGCTGCACAC GTACCACTCC 
GTGCGGGAGG CGCTGGAGCG TGAGGTGGCG CATGAGCTAG AGAAGGAAGG CGGCGGTGGT 
GAGGGTAGAG GGGAGGAGGC GTCGTCGCGG TCTCGCCCGT CCCAGGCTAG CGGCAACCAC 
AGCAACAGTC GAACGCAACT GTTTTCCAAG GCGACTGCTG CCGACGACTC GGTCAGCAAC 
TTCAGTGCCG GGAGCACCAC CACGACTATG ACGGTCATGC AGGCTCTGCG CCCACCCGCC 
GTGGTGCTGC ATCGCGTGCT GGACGAGTTT GTGTCGCTCG TGTCCTCGGA CGACTACGGC 
CCCATGCGGG AGATCGGGAA CGCGGACGAT GCTGCAGGAG CAGCCGCGGG GGCGGGCACC 
GCTGGTAACA GCGGCCGCCT CCCTACCAGT ACCTCGCCAC ACTGGATCGG GCAGGTCACT 
GTCGGCACTG TGCACCGCGC GAAGGGCATG GAGTGGCCGG CGGTGCTGCT GCCCGGGTGC 
TGGGTCGGCG AGTACCCGGT GCGGCCACGC GAAGAAGAAA AACGCGTCTT TTACGTGGGC 
ATGAGTCGAG CCATGAAGCA CCTTGTGTGC TTCACCGCAG CAACGCGCGA GTGCAGGTCT 
GGCAGCGGTC AGGCGGCAAC CACGGCAAGC GTGATCGACC TGCCCGCGCA GCACACACAA 
AGCGGAACGC TGGAGCCGAC GCCGTACCTG GCTGCCCTCG GCGACAAGCT GGAGCGGGTC 
AACTACGCCG ACCTCAAGAC AGCGTATCTG AAGGAGCAAG GGTGCCTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania.
Genome research21(12)2129-42(2011 Dec)
Rogers MB,Hilley JD,Dickens NJ,Wilkes J,Bates PA,Depledge DP,Harris D,Her Y,Herzyk P,Imamura H,Otto TD,Sanders M,Seeger K,Dujardin JC,Berriman M,Smith DF,Hertz-Fowler C,Mottram JC

Comparative genomic analysis of three Leishmania species that cause diverse human disease.
Nature genetics39(7)839-47(2007 Jul)
Peacock CS,Seeger K,Harris D,Murphy L,Ruiz JC,Quail MA,Peters N,Adlem E,Tivey A,Aslett M,Kerhornou A,Ivens A,Fraser A,Rajandream MA,Carver T,Norbertczak H,Chillingworth T,Hance Z,Jagels K,Moule S,Ormond D,Rutter S,Squares R,Whitehead S,Rabbinowitsch E,Arrowsmith C,White B,Thurston S,Bringaud F,Baldauf SL,Faulconbridge A,Jeffares D,Depledge DP,Oyola SO,Hilley JD,Brito LO,Tosi LR,Barrell B,Cruz AK,Mottram JC,Smith DF,Berriman M

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LINJ_09_0640 cDNA ORF clone, Leishmania infantum JPCM5

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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