IscW_ISCW005852 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	IscW_ISCW005852
Entrez Gene ID	8052767
Full Name	ABCA5 transporter, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029982377.1	XP_029838237.1	phospholipid-transporting ATPase ABCA1
XM_002435870.1	XP_002435915.1	ABCA5 transporter, putative

Pathways from BioSystems
Gene Ontology

The following IscW_ISCW005852 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IscW_ISCW005852 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIa13668	XM_002435870.1 Latest version!	Ixodes scapularis ABCA5 transporter, putative, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OIa25031	XM_029982377.1 Latest version!	Ixodes scapularis phospholipid-transporting ATPase ABCA1 (LOC8052767), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IscW_ISCW005852 ( XM_002435870.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002435915.1 Ixodes scapularis ABCA5 transporter, putative, mRNA.

Price & Availability↑

CloneID	OIa13668
Clone ID Related Accession (Same CDS sequence)	XM_002435870.1
Accession Version	XM_002435870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2583bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1245859200000
Organism	Ixodes scapularis(black-legged tick)
Product	ABCA5 transporter, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002656083). COMPLETENESS: incomplete on both ends.

ATGGGTCTGT CCGATCTCGC CTACTGGATG GGGCTCTTCA GCGCCAGCAT GCTGTTCTTG 
ATCGTGGACA GCATCATCGC GATCGGCTGC CTAACCATTC TAAAAGATAC GCAAACCGGC 
ACTCACTCTT ACTTCGAGAA CGTGAACCTG CTGCTGTTGG CTGTGGTATT CCTCGTATTC 
TGCTGCATGC AAACTCTCCA CGCCATGCTC ATTTCTGTCC TCTTCAAGAG TGGCGCCATG 
ACTGCCCTGT TCGGACTCAT CTACTGGCTC GTCCTGACGT TCGTCGGTCC CGTGTTCTAC 
ATCGAAGGTT TTACAGGCGG CTTGGCCAGC TACATTCTGG CTCGCCCATG GAAGAAGCTC 
GCCACGTCGA TGACCCCTTG CATCGGGACC TACTGGATGC TCAAGATACT TAGCCTGTTC 
CAGGACTACG ACGGCTACGC AGGGTGGGGC ATGGTGAACG AGTACGCACT GGATCTGGAT 
AACGTCACCA TTATGCAGAT CGTCTGCGTA ATGGGTGCCA CTTTCTTGGC CCTGGTCTGT 
CTCATCTGGT ACCTGTCGCG GGTCCTGCCT TGGGTGGCTT CTGCACCACG CCCCTTTTAC 
TTCCCGCTTC TGCCCAGCTA CTGGATTCCG TCCCTGGTCG CTCCCATGGA CAGTGAGGAG 
GAAGGGCCAG GCGGGGAAGA CGGTGTCTTC GAGCCTCTGT CCAAAGGAAT GAAGATCGTG 
ATGTCCGCCC GAGATCTATC GAAGGTCTTC GGCAGAGCGG TGGCCCTAAA TCGGATCAAC 
CTAAACATCT ACGAAGGGCA GATCACGGTC CTCCTCGGCC ACAACGGTTC GGGCAAGACG 
ACGCTCATGA ACATCTTCGC AGGTCTTACA TCTCCGTCCG GAGGGGTGGC CCGGATCTGC 
GGCTACGACG TGACCAAGAG CACGGCCCAG GCACGTCAGA GCATGGGCTA CTGCCAGCAG 
TCCAACGTTT TCTTCGACGA CCTCACAGTC CGCGAGCATC TCATCTATTT TGGAACGCTC 
AAGGGCGTCG GTTCTTCCGA GCTGGGCGCC ATGACTGACA AGATCCTCCA GGAGGTGAAG 
CTGACGAATA AAGCCGACTC ATTTCCCGAG GTTCTCTCTG GCGGCATGAA ACGGAAACTG 
GGTGTTGCCA TCGCCCTCGT TGCGAAGCCA AAGGTGCTGT TCCTGGACGA GCCTTCGTCC 
GGGATGGATG CCCAGAACCG TCGCACCATG TGGAACCTGC TCCTGGAGCT GCGCAGCAGG 
TGCACCCTGC TTCTGTCCAC GCACGACATG GAGGAGGCCG ACGCGCTCGC GGACAGGATC 
CTCGTCTTAG CCAAGGGGGC GCTCGTGTGC AGCGGGTCGC CGCCCTTTCT CATGAGGGAG 
TACGGTGCCG GTTACCAGGT GAGGATCACC AAGAGGAGCG CGGGTTTCGA CCTGCTCCAA 
ATCCAGCGAA TCATCGTTGC CACGGCGCCG GAAGCCGAAC TGGTCGAGGA GAGGGCGGGC 
GACGTGCTCA TCCGGCTGAA CGTGGTGCAC TTCACGGGCT TCGACGTCAT GTTCCGGGCC 
CTGGAGGAGA AGAAGGACCA GCTGGGCATC GAGACCATAG GCGTCACTTT GTCCACCATC 
GAAGACGTGT ACATCAAGAT CCACTTTGCA GCAGCGTCTC AGACAGGGCC GAATTCCGAC 
ATCTACGACG ATGCCAAAGT GGTCGGCAAA ACGAGGCACC ACAAGCCCGT CTCGTCTGCG 
TGCTTGAAGG CACTCTTCTT CAAGAGGGCC ATCCACTTCT CGCGCTCGTG GGATCTCTGC 
CTGGTGTCGT GTATCATGCC CTGCCTTGCC ATTCTCGCCA TCTTCGTCGT GGAGGACCTG 
CTCTTGCCGC AGCCACCCAG CGAGGTCCCC GGCGAGAAGA CCTTGCCGGT GACGCTTGAC 
GCCATCAAGC CTTCTGGTGC ACGAGTCTTT GTGCTTCAGG AGACCGGAAG AGACCAAGAG 
CTGGCAGACA TGTTCGTCCG GCTCACGGTG ACCGGAGGAC ATGCCACCGT GCGTAACTTC 
ACGTATGCCA GACGGGGCCT CTTCATGGAG GCAGCGATGG ACTTCTGGGG GTACACCCAT 
ACCTACATTC TCGGGGCTGC GTTCACGAGG GACGGGGTGG AACTCTGGTA CAGCGCGTAC 
ACCAACGGCA GCCAGGCCGT CGCGATGAAC CTGCTGACAA CCGCGCTGCT CCGACTCCGA 
ACGGGCAATC CGACGGCCGA CCTGTCGGCC ACCGTCGTGG TCCATGAGCA GTCGGTCGAT 
ACCGACCGGC ACGACGTCGA TCTGGCCTCG GAGATGATCC GCAAGAGCGT TGTCTTCCGC 
AGCTTCTCCT TCCCGGTGGT GACGTCCCTG ATCGTGGCCG GCTTCGTACT CTTCCCGGTC 
GCGGAGCGCA CGTGCCAGGC CAAGACGTTG CAGCTCATGA CCGGCATGTC GGGGAGCGCC 
TACTGGGTCT CCAACCTCAT GTTCGACCTG GTCGTGTACT GGGTCATCTG GATCTTCGCC 
GCGATGGTCG TGGTCCAATA CGTCGAGCTC TTCATCGACT CCATACGTAA TCGTGGGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002435915.1
CDS	1..2583
Translation	MGLSDLAYWMGLFSASMLFLIVDSIIAIGCLTILKDTQTGTHSYFENVNLLLLAVVFLVFCCMQTLHAMLISVLFKSGAMTALFGLIYWLVLTFVGPVFYIEGFTGGLASYILARPWKKLATSMTPCIGTYWMLKILSLFQDYDGYAGWGMVNEYALDLDNVTIMQIVCVMGATFLALVCLIWYLSRVLPWVASAPRPFYFPLLPSYWIPSLVAPMDSEEEGPGGEDGVFEPLSKGMKIVMSARDLSKVFGRAVALNRINLNIYEGQITVLLGHNGSGKTTLMNIFAGLTSPSGGVARICGYDVTKSTAQARQSMGYCQQSNVFFDDLTVREHLIYFGTLKGVGSSELGAMTDKILQEVKLTNKADSFPEVLSGGMKRKLGVAIALVAKPKVLFLDEPSSGMDAQNRRTMWNLLLELRSRCTLLLSTHDMEEADALADRILVLAKGALVCSGSPPFLMREYGAGYQVRITKRSAGFDLLQIQRIIVATAPEAELVEERAGDVLIRLNVVHFTGFDVMFRALEEKKDQLGIETIGVTLSTIEDVYIKIHFAAASQTGPNSDIYDDAKVVGKTRHHKPVSSACLKALFFKRAIHFSRSWDLCLVSCIMPCLAILAIFVVEDLLLPQPPSEVPGEKTLPVTLDAIKPSGARVFVLQETGRDQELADMFVRLTVTGGHATVRNFTYARRGLFMEAAMDFWGYTHTYILGAAFTRDGVELWYSAYTNGSQAVAMNLLTTALLRLRTGNPTADLSATVVVHEQSVDTDRHDVDLASEMIRKSVVFRSFSFPVVTSLIVAGFVLFPVAERTCQAKTLQLMTGMSGSAYWVSNLMFDLVVYWVIWIFAAMVVVQYVELFIDSIRNRGD

Target ORF information:

RefSeq Version	XM_002435870.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis ABCA5 transporter, putative, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002435870.1

ORF Insert Sequence:

ATGGGTCTGT CCGATCTCGC CTACTGGATG GGGCTCTTCA GCGCCAGCAT GCTGTTCTTG 
ATCGTGGACA GCATCATCGC GATCGGCTGC CTAACCATTC TAAAAGATAC GCAAACCGGC 
ACTCACTCTT ACTTCGAGAA CGTGAACCTG CTGCTGTTGG CTGTGGTATT CCTCGTATTC 
TGCTGCATGC AAACTCTCCA CGCCATGCTC ATTTCTGTCC TCTTCAAGAG TGGCGCCATG 
ACTGCCCTGT TCGGACTCAT CTACTGGCTC GTCCTGACGT TCGTCGGTCC CGTGTTCTAC 
ATCGAAGGTT TTACAGGCGG CTTGGCCAGC TACATTCTGG CTCGCCCATG GAAGAAGCTC 
GCCACGTCGA TGACCCCTTG CATCGGGACC TACTGGATGC TCAAGATACT TAGCCTGTTC 
CAGGACTACG ACGGCTACGC AGGGTGGGGC ATGGTGAACG AGTACGCACT GGATCTGGAT 
AACGTCACCA TTATGCAGAT CGTCTGCGTA ATGGGTGCCA CTTTCTTGGC CCTGGTCTGT 
CTCATCTGGT ACCTGTCGCG GGTCCTGCCT TGGGTGGCTT CTGCACCACG CCCCTTTTAC 
TTCCCGCTTC TGCCCAGCTA CTGGATTCCG TCCCTGGTCG CTCCCATGGA CAGTGAGGAG 
GAAGGGCCAG GCGGGGAAGA CGGTGTCTTC GAGCCTCTGT CCAAAGGAAT GAAGATCGTG 
ATGTCCGCCC GAGATCTATC GAAGGTCTTC GGCAGAGCGG TGGCCCTAAA TCGGATCAAC 
CTAAACATCT ACGAAGGGCA GATCACGGTC CTCCTCGGCC ACAACGGTTC GGGCAAGACG 
ACGCTCATGA ACATCTTCGC AGGTCTTACA TCTCCGTCCG GAGGGGTGGC CCGGATCTGC 
GGCTACGACG TGACCAAGAG CACGGCCCAG GCACGTCAGA GCATGGGCTA CTGCCAGCAG 
TCCAACGTTT TCTTCGACGA CCTCACAGTC CGCGAGCATC TCATCTATTT TGGAACGCTC 
AAGGGCGTCG GTTCTTCCGA GCTGGGCGCC ATGACTGACA AGATCCTCCA GGAGGTGAAG 
CTGACGAATA AAGCCGACTC ATTTCCCGAG GTTCTCTCTG GCGGCATGAA ACGGAAACTG 
GGTGTTGCCA TCGCCCTCGT TGCGAAGCCA AAGGTGCTGT TCCTGGACGA GCCTTCGTCC 
GGGATGGATG CCCAGAACCG TCGCACCATG TGGAACCTGC TCCTGGAGCT GCGCAGCAGG 
TGCACCCTGC TTCTGTCCAC GCACGACATG GAGGAGGCCG ACGCGCTCGC GGACAGGATC 
CTCGTCTTAG CCAAGGGGGC GCTCGTGTGC AGCGGGTCGC CGCCCTTTCT CATGAGGGAG 
TACGGTGCCG GTTACCAGGT GAGGATCACC AAGAGGAGCG CGGGTTTCGA CCTGCTCCAA 
ATCCAGCGAA TCATCGTTGC CACGGCGCCG GAAGCCGAAC TGGTCGAGGA GAGGGCGGGC 
GACGTGCTCA TCCGGCTGAA CGTGGTGCAC TTCACGGGCT TCGACGTCAT GTTCCGGGCC 
CTGGAGGAGA AGAAGGACCA GCTGGGCATC GAGACCATAG GCGTCACTTT GTCCACCATC 
GAAGACGTGT ACATCAAGAT CCACTTTGCA GCAGCGTCTC AGACAGGGCC GAATTCCGAC 
ATCTACGACG ATGCCAAAGT GGTCGGCAAA ACGAGGCACC ACAAGCCCGT CTCGTCTGCG 
TGCTTGAAGG CACTCTTCTT CAAGAGGGCC ATCCACTTCT CGCGCTCGTG GGATCTCTGC 
CTGGTGTCGT GTATCATGCC CTGCCTTGCC ATTCTCGCCA TCTTCGTCGT GGAGGACCTG 
CTCTTGCCGC AGCCACCCAG CGAGGTCCCC GGCGAGAAGA CCTTGCCGGT GACGCTTGAC 
GCCATCAAGC CTTCTGGTGC ACGAGTCTTT GTGCTTCAGG AGACCGGAAG AGACCAAGAG 
CTGGCAGACA TGTTCGTCCG GCTCACGGTG ACCGGAGGAC ATGCCACCGT GCGTAACTTC 
ACGTATGCCA GACGGGGCCT CTTCATGGAG GCAGCGATGG ACTTCTGGGG GTACACCCAT 
ACCTACATTC TCGGGGCTGC GTTCACGAGG GACGGGGTGG AACTCTGGTA CAGCGCGTAC 
ACCAACGGCA GCCAGGCCGT CGCGATGAAC CTGCTGACAA CCGCGCTGCT CCGACTCCGA 
ACGGGCAATC CGACGGCCGA CCTGTCGGCC ACCGTCGTGG TCCATGAGCA GTCGGTCGAT 
ACCGACCGGC ACGACGTCGA TCTGGCCTCG GAGATGATCC GCAAGAGCGT TGTCTTCCGC 
AGCTTCTCCT TCCCGGTGGT GACGTCCCTG ATCGTGGCCG GCTTCGTACT CTTCCCGGTC 
GCGGAGCGCA CGTGCCAGGC CAAGACGTTG CAGCTCATGA CCGGCATGTC GGGGAGCGCC 
TACTGGGTCT CCAACCTCAT GTTCGACCTG GTCGTGTACT GGGTCATCTG GATCTTCGCC 
GCGATGGTCG TGGTCCAATA CGTCGAGCTC TTCATCGACT CCATACGTAA TCGTGGGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IscW_ISCW005852 ( XM_029982377.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029838237.1 Ixodes scapularis phospholipid-transporting ATPase ABCA1 (LOC8052767), mRNA.

Price & Availability↑

CloneID	OIa25031
Clone ID Related Accession (Same CDS sequence)	XM_029982377.1
Accession Version	XM_029982377.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	phospholipid-transporting ATPase ABCA1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919929.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGGGTCTGT CCGATCTCGC TTACTGGATG GGGCTCTTCA GCTCCAGCAT GCTGTTCTTG 
ATCGTGGACA GCATCATCGC GATCGGCTGC CTAACCATTC TAAAAGATAC GCAAACCGGC 
ACTCACTCAT ACTTCGAGAA TGTGAACCTG CTGCTGTTGG CTGTGGTATT CCTCGTATTC 
TGCTGCATGC AAACTCTCCA CGCCATGCTC ATTTCTGTCC TCTTCAAGAG TGGCGCCATG 
ACTGCCCTGT TCGGACTCCT CTACTGGCTC GTTCTGACGT TCGTCGGTCC CGCGTTCTAC 
ATCGAAGGTT TTACAGGCGG CTTGGCCAGC TACATTCTGG CTCGCCCGTG GAAGAAGCTC 
GCCACGTCGA TGACCCCTTG CATCGGGACC TACTGGATGC TCAAGATACT TAGCCTGTTC 
CAGGACTACG ACGGCTACGC AGGGTGGGGC ATGGTGAACG AGTACGCACT GGATCTGGAT 
AACGTCACCA TTATGCAGAT CCTCTGTGTA ATGTATGCCA CTTTCTTGGC TCTGGTCTGT 
CTCATCTGGT ACCTGTCGCG GGTCCTGCCC TGGGTGGCTT CTGCACCACG CCCCTTTTAC 
TTCCCGCTTC TGCCCAGCTA CTGGATTCCG TCCCTGGTCG CTCCCATGGA CAGTGAGGAG 
GAAGGGCCAG GCGGGGAAGA CGGTGTCTTC GAGCCTCTGC CCAAAGGAAT GAAGATCGTG 
ATGTCCGCCC GAGATTTATC GAAGGTCTTC GGCAGAGCGG TGGCCCTAAA TAGGATCAAC 
CTAAACATCT ACGAAGGGCA GATCACGGTC CTCCTCGGCC ACAACGGTTC GGGCAAGACG 
ACGCTCATGA ACATCTTCGC AGGTCTTACA TCTCCGTCCG GAGGGGTGGC CCGGATCTGC 
GGCTACGACG TGACCAAGAG CACGGCCCAG GCACGTCAGA GCATGGGCTA CTGCCAGCAG 
TCCAACGTTT TCTTCGACGA CCTCACAGTC CGCGAGCATC TCATCTATTT CGGAACGCTC 
AAGGGCGTCG GTTCTTCCGA GCTGGGCGCC ATGACTGACA AGATCCTCCA GGATGTGAAG 
CTGACGAATA AAGCCGACTC ATTTCCCGAG GTTCTCTCTG GCGGCATGAA ACGGAAACTG 
GGAGTTGCCA TCGCCCTCGT TGCGAAGCCA AAGGTGCTGT TCCTGGACGA GCCTTCGTCC 
GGGATGGACG CCCAGAACCG TCGCATTATG TGGAACCTGC TCCTGGAGCT GCGCAGCAGG 
TGCACCCTGC TTCTGTCCAC GCACGACATG GAGGAGGCCG ACGCGCTCGC GGACAGGATC 
CTCGTCTTAG CCAAGGGGGC GCTCGTGTGC AGCGGGTCGC CGCCCTTTCT CATGAGGGAG 
TACGGCGCCG GTTACCAGGT GAGGATCACC AAGAGGAGCA CGGGTTTCGA CCTGCTCCAG 
ATCCAGCGAA TCATCGTTGC CACGGCGCCG GAAGCCGAAC TGGTCGAGGA GAGGGCGGGC 
GACGTGCTCA TCCGCCTGAA CGTGGTGCAC TTCACGGGCT TCGACGTCAT GTTCCGGGCC 
CTGGAGGAGA AGAAGGACCA ACTGGGCATC GAGACCATAG GCGTCACTCT GTCCACCATC 
GAAGACGTGT ACATCAAGAT CCACTTTGCA GCAGCGTCTC AGACAGGGCA AAATTCCGAC 
ATCTACGAGG ATGCCAAAGT GGTCGGCAAA ACGAGGCACC ACAAGCCCGT CTCGTCTGCG 
TGCTTGAAGG CACTCTTCAT CAAGAGGGCC ATCCACTTCT CGCGCTCGTG GGATCTCTGC 
ATGGTGTCCT GTATCATGCC CTGCCTTGCC ATTCTCGCCA TCTTCGTCGT GGAGGACCTG 
CTCTTGCCGC AGCCACCCAG CGAGGTCCCC GGCGAGATGA CCTTGCCGGT GACGCTTGAC 
ACCATCAAGC CTTCTGATGC ACGAGTGTTT GTGCTTCAGG AGACCGGAAG AGACCAGGAG 
CTGGCAGACA TGTTCGCCCG GCTTACGGTG ACCGGAGGAC GTGCCACCGT TCGTAACTTC 
ACGGATGCCA GACGGGGCCT CTTCATGGAG GCAGCGATGG ACTTCTGGGG GTACACCCAG 
ACCTACATTC TCGGGGCTGC GTTCACGAGG GACGGGGTGG AACTCTGGTA CAGCGCGTAC 
ACCAACGGCA GCCAGGCCGT CGCGATGAAC CTGCTGACAA CCGCGCTGCT CCGACTCCGA 
ACGGGCAATC CGACGGCCGA CCTGTCGGCC ACCGTCGTGG TCCATGAGCA GCCGATCGAT 
ACCGACCGGC ACGACGTCGA TCTGGCCTCG GAGATGATCC GCAAGAGCGT TGTCTTCCGC 
AGCTTCTCCT TCCCGGTGGT GACGTCCCTG ATTGTGGCCG GCTTCGTCCT CTTCCCGGTC 
GCAGAGCGCA CGTGCCAGGC CAAGACGTTG CAGCTCATGA CCGGCATGTC GGGGAGCGCC 
TACTGGGGCT CCAACCTCGT GTTCGACCTG GTCGTGTACT GGGTCATCTG GATCTTCGCC 
GCGATGGTCG CGGTCCAATA CGTCGAGCTC TTCATCGACT CCATACTTGC CCTGATTGTG 
GTCATCGGAG TGTTTTCGGG CTTGGCTCTT CCGTGTGCCT ACCTTCTGTC CCGGAGAGCA 
AATACGCAGT ACGGGGCCTT TGCCATCGTC ACAATCACCT TCAGCTTCTT CGGTACAAGC 
TTGCTCATCC TGTCGGGTCT CACGGGGGCC TACGAGCGCG ACGAACAAGT GACGTTGGAC 
TACGTCTACA TGATGCTCCC GCCGTACGCC TTCCCCAGCT GCCTCATCAA GATCCTGAAG 
CTAGAGATGG AGAACAGGGT CTGCCTCAAA CACAGGCACA ACATCATCAA AGCCAAGTGC 
AAAAACCTGC GCAACGAAAT GTGGGGACAC ATGCTCTACT GCTGCTCTAC CACAGAGGAG 
GAGAGCGACT ATTGGTCAGC TGTGCATCCT TTGGGCTTCA GCCAGAGGGG CGTCGGCTAT 
GAGGTGATTG TCATGGCCAT TGAGGGGACG CTGCTGTTCC TCCTGCTCAG CTGGCTGGAC 
TCCGGGTCCC TCCCCATCAG CGAGGCGTAC GACCCCGACA AGCACAAGGC TAACGAAAGC 
CTGGATAGAG ACGTGATGGC CGAGAAGGAT ACAGTGCGGA ACATCGTGTC CAAGAAGATG 
TTCTCCAAGG CGGCTCTGAC GGTAGAGGAT CTACACAAGA AGTTCCCAAC CGTGCACGCC 
GTCAAGGGAC TGTCCTTCAC TGTCCTGTCC AAGGAGTGCT TTGGTTTCCT TGGCTCAAAT 
GGCGCCGGCA AGACCACCGC CTTCCAGATG CTCGCAGCAC TTATTCCTGC GAGCCGCGGA 
GAGGCGTACA TGAGGAGGTG CATCCTGTCT TGGGAAGCCA GGAAGTGGCA GTCCAAGGTC 
GGATACTGCC CTCAGTATGG CGGACTGCTG GAGACCCTGA CTGCCCGCGA GTACCTGAGA 
CTGTTCGCCT CCCTGCGCGG CGTCTCGACC CACAAGATCG ATCAGCTGGT CGAGAGCGTC 
TTTAACCTCA TGGGGCTCGA CGGCATCGCG GACAACCGGT GTGGAACTTA CAGCGGGGGC 
AACAAGCGCA AGTTGAGCAT GGCTGTGGCC ATCATCGGCC TCCCGAGGCT CGTGCTTCTG 
GACGAGCCCA CGGCCGGCGT GGACGTGGTC GCCAGGAGGC GGATGCTGTC CGGCCTCAAG 
GTCGTCATGG ACTACTCGGA CATGTCCGTC GTCCTCACGT CGCAGAAGAT GGACGAGTGC 
GAGACGGCTT GCGACCGGAT CGGCATCGTG CTGGCCGGTC AGTTCCGCTG CCTCGGCACC 
CTCCAGCACC TCAAGGACAA GTACGGCACG GGCTCCACCA TCAAGTTGAA GCTGCAGAGC 
AGGGACGCCC AGCTGCGCAG GAGCGTCGAA GACGCCATCG TGGCCCAGTT CCCGGGCATC 
CAGCTCATGG ACTTCCACCA GGGCATCTTT GTGTTCCGCC TGGAAGAGAA GCTTCCCTGG 
AGTGTCCTCT TCTCCAAGAT GCTCGAGTTG CAGTCGCAGT TCGTGTTCGA GTACGTGCTG 
GTGTCGGACA CGACGCTCGA GCAGATCTTC ATCAACTTTG CCCGGAGCTA CGAGAAGGAG 
GAGCGGGGCA AGGCCGCCGA GACTTCCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029838237.1
CDS	1..4173
Translation	MGLSDLAYWMGLFSSSMLFLIVDSIIAIGCLTILKDTQTGTHSYFENVNLLLLAVVFLVFCCMQTLHAMLISVLFKSGAMTALFGLLYWLVLTFVGPAFYIEGFTGGLASYILARPWKKLATSMTPCIGTYWMLKILSLFQDYDGYAGWGMVNEYALDLDNVTIMQILCVMYATFLALVCLIWYLSRVLPWVASAPRPFYFPLLPSYWIPSLVAPMDSEEEGPGGEDGVFEPLPKGMKIVMSARDLSKVFGRAVALNRINLNIYEGQITVLLGHNGSGKTTLMNIFAGLTSPSGGVARICGYDVTKSTAQARQSMGYCQQSNVFFDDLTVREHLIYFGTLKGVGSSELGAMTDKILQDVKLTNKADSFPEVLSGGMKRKLGVAIALVAKPKVLFLDEPSSGMDAQNRRIMWNLLLELRSRCTLLLSTHDMEEADALADRILVLAKGALVCSGSPPFLMREYGAGYQVRITKRSTGFDLLQIQRIIVATAPEAELVEERAGDVLIRLNVVHFTGFDVMFRALEEKKDQLGIETIGVTLSTIEDVYIKIHFAAASQTGQNSDIYEDAKVVGKTRHHKPVSSACLKALFIKRAIHFSRSWDLCMVSCIMPCLAILAIFVVEDLLLPQPPSEVPGEMTLPVTLDTIKPSDARVFVLQETGRDQELADMFARLTVTGGRATVRNFTDARRGLFMEAAMDFWGYTQTYILGAAFTRDGVELWYSAYTNGSQAVAMNLLTTALLRLRTGNPTADLSATVVVHEQPIDTDRHDVDLASEMIRKSVVFRSFSFPVVTSLIVAGFVLFPVAERTCQAKTLQLMTGMSGSAYWGSNLVFDLVVYWVIWIFAAMVAVQYVELFIDSILALIVVIGVFSGLALPCAYLLSRRANTQYGAFAIVTITFSFFGTSLLILSGLTGAYERDEQVTLDYVYMMLPPYAFPSCLIKILKLEMENRVCLKHRHNIIKAKCKNLRNEMWGHMLYCCSTTEEESDYWSAVHPLGFSQRGVGYEVIVMAIEGTLLFLLLSWLDSGSLPISEAYDPDKHKANESLDRDVMAEKDTVRNIVSKKMFSKAALTVEDLHKKFPTVHAVKGLSFTVLSKECFGFLGSNGAGKTTAFQMLAALIPASRGEAYMRRCILSWEARKWQSKVGYCPQYGGLLETLTAREYLRLFASLRGVSTHKIDQLVESVFNLMGLDGIADNRCGTYSGGNKRKLSMAVAIIGLPRLVLLDEPTAGVDVVARRRMLSGLKVVMDYSDMSVVLTSQKMDECETACDRIGIVLAGQFRCLGTLQHLKDKYGTGSTIKLKLQSRDAQLRRSVEDAIVAQFPGIQLMDFHQGIFVFRLEEKLPWSVLFSKMLELQSQFVFEYVLVSDTTLEQIFINFARSYEKEERGKAAETSS

Target ORF information:

RefSeq Version	XM_029982377.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis phospholipid-transporting ATPase ABCA1 (LOC8052767), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029982377.1

ORF Insert Sequence:

ATGGGTCTGT CCGATCTCGC TTACTGGATG GGGCTCTTCA GCTCCAGCAT GCTGTTCTTG 
ATCGTGGACA GCATCATCGC GATCGGCTGC CTAACCATTC TAAAAGATAC GCAAACCGGC 
ACTCACTCAT ACTTCGAGAA TGTGAACCTG CTGCTGTTGG CTGTGGTATT CCTCGTATTC 
TGCTGCATGC AAACTCTCCA CGCCATGCTC ATTTCTGTCC TCTTCAAGAG TGGCGCCATG 
ACTGCCCTGT TCGGACTCCT CTACTGGCTC GTTCTGACGT TCGTCGGTCC CGCGTTCTAC 
ATCGAAGGTT TTACAGGCGG CTTGGCCAGC TACATTCTGG CTCGCCCGTG GAAGAAGCTC 
GCCACGTCGA TGACCCCTTG CATCGGGACC TACTGGATGC TCAAGATACT TAGCCTGTTC 
CAGGACTACG ACGGCTACGC AGGGTGGGGC ATGGTGAACG AGTACGCACT GGATCTGGAT 
AACGTCACCA TTATGCAGAT CCTCTGTGTA ATGTATGCCA CTTTCTTGGC TCTGGTCTGT 
CTCATCTGGT ACCTGTCGCG GGTCCTGCCC TGGGTGGCTT CTGCACCACG CCCCTTTTAC 
TTCCCGCTTC TGCCCAGCTA CTGGATTCCG TCCCTGGTCG CTCCCATGGA CAGTGAGGAG 
GAAGGGCCAG GCGGGGAAGA CGGTGTCTTC GAGCCTCTGC CCAAAGGAAT GAAGATCGTG 
ATGTCCGCCC GAGATTTATC GAAGGTCTTC GGCAGAGCGG TGGCCCTAAA TAGGATCAAC 
CTAAACATCT ACGAAGGGCA GATCACGGTC CTCCTCGGCC ACAACGGTTC GGGCAAGACG 
ACGCTCATGA ACATCTTCGC AGGTCTTACA TCTCCGTCCG GAGGGGTGGC CCGGATCTGC 
GGCTACGACG TGACCAAGAG CACGGCCCAG GCACGTCAGA GCATGGGCTA CTGCCAGCAG 
TCCAACGTTT TCTTCGACGA CCTCACAGTC CGCGAGCATC TCATCTATTT CGGAACGCTC 
AAGGGCGTCG GTTCTTCCGA GCTGGGCGCC ATGACTGACA AGATCCTCCA GGATGTGAAG 
CTGACGAATA AAGCCGACTC ATTTCCCGAG GTTCTCTCTG GCGGCATGAA ACGGAAACTG 
GGAGTTGCCA TCGCCCTCGT TGCGAAGCCA AAGGTGCTGT TCCTGGACGA GCCTTCGTCC 
GGGATGGACG CCCAGAACCG TCGCATTATG TGGAACCTGC TCCTGGAGCT GCGCAGCAGG 
TGCACCCTGC TTCTGTCCAC GCACGACATG GAGGAGGCCG ACGCGCTCGC GGACAGGATC 
CTCGTCTTAG CCAAGGGGGC GCTCGTGTGC AGCGGGTCGC CGCCCTTTCT CATGAGGGAG 
TACGGCGCCG GTTACCAGGT GAGGATCACC AAGAGGAGCA CGGGTTTCGA CCTGCTCCAG 
ATCCAGCGAA TCATCGTTGC CACGGCGCCG GAAGCCGAAC TGGTCGAGGA GAGGGCGGGC 
GACGTGCTCA TCCGCCTGAA CGTGGTGCAC TTCACGGGCT TCGACGTCAT GTTCCGGGCC 
CTGGAGGAGA AGAAGGACCA ACTGGGCATC GAGACCATAG GCGTCACTCT GTCCACCATC 
GAAGACGTGT ACATCAAGAT CCACTTTGCA GCAGCGTCTC AGACAGGGCA AAATTCCGAC 
ATCTACGAGG ATGCCAAAGT GGTCGGCAAA ACGAGGCACC ACAAGCCCGT CTCGTCTGCG 
TGCTTGAAGG CACTCTTCAT CAAGAGGGCC ATCCACTTCT CGCGCTCGTG GGATCTCTGC 
ATGGTGTCCT GTATCATGCC CTGCCTTGCC ATTCTCGCCA TCTTCGTCGT GGAGGACCTG 
CTCTTGCCGC AGCCACCCAG CGAGGTCCCC GGCGAGATGA CCTTGCCGGT GACGCTTGAC 
ACCATCAAGC CTTCTGATGC ACGAGTGTTT GTGCTTCAGG AGACCGGAAG AGACCAGGAG 
CTGGCAGACA TGTTCGCCCG GCTTACGGTG ACCGGAGGAC GTGCCACCGT TCGTAACTTC 
ACGGATGCCA GACGGGGCCT CTTCATGGAG GCAGCGATGG ACTTCTGGGG GTACACCCAG 
ACCTACATTC TCGGGGCTGC GTTCACGAGG GACGGGGTGG AACTCTGGTA CAGCGCGTAC 
ACCAACGGCA GCCAGGCCGT CGCGATGAAC CTGCTGACAA CCGCGCTGCT CCGACTCCGA 
ACGGGCAATC CGACGGCCGA CCTGTCGGCC ACCGTCGTGG TCCATGAGCA GCCGATCGAT 
ACCGACCGGC ACGACGTCGA TCTGGCCTCG GAGATGATCC GCAAGAGCGT TGTCTTCCGC 
AGCTTCTCCT TCCCGGTGGT GACGTCCCTG ATTGTGGCCG GCTTCGTCCT CTTCCCGGTC 
GCAGAGCGCA CGTGCCAGGC CAAGACGTTG CAGCTCATGA CCGGCATGTC GGGGAGCGCC 
TACTGGGGCT CCAACCTCGT GTTCGACCTG GTCGTGTACT GGGTCATCTG GATCTTCGCC 
GCGATGGTCG CGGTCCAATA CGTCGAGCTC TTCATCGACT CCATACTTGC CCTGATTGTG 
GTCATCGGAG TGTTTTCGGG CTTGGCTCTT CCGTGTGCCT ACCTTCTGTC CCGGAGAGCA 
AATACGCAGT ACGGGGCCTT TGCCATCGTC ACAATCACCT TCAGCTTCTT CGGTACAAGC 
TTGCTCATCC TGTCGGGTCT CACGGGGGCC TACGAGCGCG ACGAACAAGT GACGTTGGAC 
TACGTCTACA TGATGCTCCC GCCGTACGCC TTCCCCAGCT GCCTCATCAA GATCCTGAAG 
CTAGAGATGG AGAACAGGGT CTGCCTCAAA CACAGGCACA ACATCATCAA AGCCAAGTGC 
AAAAACCTGC GCAACGAAAT GTGGGGACAC ATGCTCTACT GCTGCTCTAC CACAGAGGAG 
GAGAGCGACT ATTGGTCAGC TGTGCATCCT TTGGGCTTCA GCCAGAGGGG CGTCGGCTAT 
GAGGTGATTG TCATGGCCAT TGAGGGGACG CTGCTGTTCC TCCTGCTCAG CTGGCTGGAC 
TCCGGGTCCC TCCCCATCAG CGAGGCGTAC GACCCCGACA AGCACAAGGC TAACGAAAGC 
CTGGATAGAG ACGTGATGGC CGAGAAGGAT ACAGTGCGGA ACATCGTGTC CAAGAAGATG 
TTCTCCAAGG CGGCTCTGAC GGTAGAGGAT CTACACAAGA AGTTCCCAAC CGTGCACGCC 
GTCAAGGGAC TGTCCTTCAC TGTCCTGTCC AAGGAGTGCT TTGGTTTCCT TGGCTCAAAT 
GGCGCCGGCA AGACCACCGC CTTCCAGATG CTCGCAGCAC TTATTCCTGC GAGCCGCGGA 
GAGGCGTACA TGAGGAGGTG CATCCTGTCT TGGGAAGCCA GGAAGTGGCA GTCCAAGGTC 
GGATACTGCC CTCAGTATGG CGGACTGCTG GAGACCCTGA CTGCCCGCGA GTACCTGAGA 
CTGTTCGCCT CCCTGCGCGG CGTCTCGACC CACAAGATCG ATCAGCTGGT CGAGAGCGTC 
TTTAACCTCA TGGGGCTCGA CGGCATCGCG GACAACCGGT GTGGAACTTA CAGCGGGGGC 
AACAAGCGCA AGTTGAGCAT GGCTGTGGCC ATCATCGGCC TCCCGAGGCT CGTGCTTCTG 
GACGAGCCCA CGGCCGGCGT GGACGTGGTC GCCAGGAGGC GGATGCTGTC CGGCCTCAAG 
GTCGTCATGG ACTACTCGGA CATGTCCGTC GTCCTCACGT CGCAGAAGAT GGACGAGTGC 
GAGACGGCTT GCGACCGGAT CGGCATCGTG CTGGCCGGTC AGTTCCGCTG CCTCGGCACC 
CTCCAGCACC TCAAGGACAA GTACGGCACG GGCTCCACCA TCAAGTTGAA GCTGCAGAGC 
AGGGACGCCC AGCTGCGCAG GAGCGTCGAA GACGCCATCG TGGCCCAGTT CCCGGGCATC 
CAGCTCATGG ACTTCCACCA GGGCATCTTT GTGTTCCGCC TGGAAGAGAA GCTTCCCTGG 
AGTGTCCTCT TCTCCAAGAT GCTCGAGTTG CAGTCGCAGT TCGTGTTCGA GTACGTGCTG 
GTGTCGGACA CGACGCTCGA GCAGATCTTC ATCAACTTTG CCCGGAGCTA CGAGAAGGAG 
GAGCGGGGCA AGGCCGCCGA GACTTCCTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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IscW_ISCW005852 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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