IscW_ISCW023403 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	IscW_ISCW023403
Entrez Gene ID	8041994
Full Name	neural cell adhesion molecule L1, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029980640.1	XP_029836500.1	neogenin isoform X2
XM_029980639.1	XP_029836499.1	neogenin isoform X1
XM_002415139.1	XP_002415184.1	neural cell adhesion molecule L1, putative

Pathways from BioSystems
Gene Ontology

The following IscW_ISCW023403 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IscW_ISCW023403 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa12179	XM_002415139.1 Latest version!	Ixodes scapularis neural cell adhesion molecule L1, putative, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OIa23562	XM_029980639.1 Latest version!	Ixodes scapularis neogenin (LOC8041994), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OIa23563	XM_029980640.1 Latest version!	Ixodes scapularis neogenin (LOC8041994), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IscW_ISCW023403 ( XM_002415139.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002415184.1 Ixodes scapularis neural cell adhesion molecule L1, putative, mRNA.

Price & Availability↑

CloneID	OIa12179
Clone ID Related Accession (Same CDS sequence)	XM_002415139.1
Accession Version	XM_002415139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1245600000000
Organism	Ixodes scapularis(black-legged tick)
Product	neural cell adhesion molecule L1, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002842287). COMPLETENESS: incomplete on both ends.

ATGGACGTCA CCGGAGGTGG CCTCTCGTGG CGCGGACACG GGCCCGCGTG TCCGGTCGCC 
TTCCCCGGGG CAATCGCCGG CGGCGCGGAC GCGGAGCACC GGAAGGGGTT CTCCGACCGG 
GGGCCAAGTG ATAACGTGTT CTTCCCGGAC TTCCGGTTTC TGGAGGAGCC TTCGGACATG 
GTGGCCATCA AGGACAGCCC TGCGATGCTC AACTGTTCGG CAGCCGGGGA TCCCGAGCCC 
ACCATTGAAT GGAAGCGGGA AGGCTCGCTA CTCCAGCTCG ATGGCGATCC AAGAAGGTCA 
ATCCTCTCCA ATGGCTCCCT CTTCTTGCGG AGTGTGCACC ATACAAGAAC AGAGAGACCC 
GACGAGGGAG TCTACCAGTG CTTGGCCACC ATTCCAAATG TTGGCACCAT CCTGAGTCGC 
TCTGCAAAGC TCCAAGTGGC TGCTCTGCCA AGGTTTGAAG AGCAGCCGAG GGACATGCGG 
CTGTTCCCGG GGCAGACGGC ATACTTCCCC TGCTCGGCCT TTGCGGTCCC TTCGGCCAGC 
GTGGTGTGGC TCAAGGACCA GCAGCCGCTG CAGCTTGACC CCTCCCGGAT GCTGGTGCTG 
CCCAGCGGGG CCCTCGAGAT TGACGGGGTG CAGACCACCG ACGAGGGGGC CTACCAGTGC 
ACCGCCTACA ATGCCGACAA GAACCGGATC AGCTCTGCGG GGAACCTCTT CGTCAGCTTG 
TCCTACGGTG AGGGGTCAGA GATATCGGCT CCAATTTTTG TGGCAGCCCC GCGGAATATA 
GTAGTTGTGA AAGGAACAAA CATGACTCTC GACTGTGCTG CAAATGGAAA CCCTCGTCCG 
AACCTGACGT GGCTCAAGGA TGGTGTAACC ATCGACATGT CGCTTCTTGA TAGCAGGTTT 
AGAAAAGTCG GAGTAGGAAG TCTGCAAATT GAAAATATCC AGGAAGCGGA CGAAGGAACC 
TACATGTGCA GAGCAGAAAA TCACGAAGAC TCTGTGGATG CCAGTGCCAC CATTGAAATC 
CAAGTTCCTC CAAGGTTCAG AAAGAAACCG AAGAACAAGT TTGCATACGA GAAGGAAGAC 
GTTGAGTTTG AGTGCGAGGT GTACGGAAAG CCGGAGCCCT CGGTGCAGTG GTTCCGTAAC 
GGGGAAGCCA TCATCCAGAG TGAATACTTC CAAATCGTCA ACGGCAACAG CTTGAGGATA 
CTGGGTCTCG TGGACTCCGA CCGAGGAATG TACCAGTGCT TCGGCTCCAA CCCGGCCGGT 
AATGTCCAGA CATCGGCCCA ACTCGTGGTT CTCGACCCAG GGGAGCTGCA CAGGGGCAAG 
AACGTCCCCT CCGCCCCTCG GGAGGTCATG GCACGGCTCG TGTCCACTCG CTTTGCCACG 
CTCTCGTGGA CGGTGCCCGA GAAAGTGGAC GGAAGCATCC TCGCTTACTC CGTCTACTAC 
AAGGAGGAAG GCTCCAGCCG GTCAGTGGTG GTCCTTGAAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002415184.1
CDS	1..1485
Translation	MDVTGGGLSWRGHGPACPVAFPGAIAGGADAEHRKGFSDRGPSDNVFFPDFRFLEEPSDMVAIKDSPAMLNCSAAGDPEPTIEWKREGSLLQLDGDPRRSILSNGSLFLRSVHHTRTERPDEGVYQCLATIPNVGTILSRSAKLQVAALPRFEEQPRDMRLFPGQTAYFPCSAFAVPSASVVWLKDQQPLQLDPSRMLVLPSGALEIDGVQTTDEGAYQCTAYNADKNRISSAGNLFVSLSYGEGSEISAPIFVAAPRNIVVVKGTNMTLDCAANGNPRPNLTWLKDGVTIDMSLLDSRFRKVGVGSLQIENIQEADEGTYMCRAENHEDSVDASATIEIQVPPRFRKKPKNKFAYEKEDVEFECEVYGKPEPSVQWFRNGEAIIQSEYFQIVNGNSLRILGLVDSDRGMYQCFGSNPAGNVQTSAQLVVLDPGELHRGKNVPSAPREVMARLVSTRFATLSWTVPEKVDGSILAYSVYYKEEGSSRSVVVLEP

Target ORF information:

RefSeq Version	XM_002415139.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis neural cell adhesion molecule L1, putative, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002415139.1

ORF Insert Sequence:

ATGGACGTCA CCGGAGGTGG CCTCTCGTGG CGCGGACACG GGCCCGCGTG TCCGGTCGCC 
TTCCCCGGGG CAATCGCCGG CGGCGCGGAC GCGGAGCACC GGAAGGGGTT CTCCGACCGG 
GGGCCAAGTG ATAACGTGTT CTTCCCGGAC TTCCGGTTTC TGGAGGAGCC TTCGGACATG 
GTGGCCATCA AGGACAGCCC TGCGATGCTC AACTGTTCGG CAGCCGGGGA TCCCGAGCCC 
ACCATTGAAT GGAAGCGGGA AGGCTCGCTA CTCCAGCTCG ATGGCGATCC AAGAAGGTCA 
ATCCTCTCCA ATGGCTCCCT CTTCTTGCGG AGTGTGCACC ATACAAGAAC AGAGAGACCC 
GACGAGGGAG TCTACCAGTG CTTGGCCACC ATTCCAAATG TTGGCACCAT CCTGAGTCGC 
TCTGCAAAGC TCCAAGTGGC TGCTCTGCCA AGGTTTGAAG AGCAGCCGAG GGACATGCGG 
CTGTTCCCGG GGCAGACGGC ATACTTCCCC TGCTCGGCCT TTGCGGTCCC TTCGGCCAGC 
GTGGTGTGGC TCAAGGACCA GCAGCCGCTG CAGCTTGACC CCTCCCGGAT GCTGGTGCTG 
CCCAGCGGGG CCCTCGAGAT TGACGGGGTG CAGACCACCG ACGAGGGGGC CTACCAGTGC 
ACCGCCTACA ATGCCGACAA GAACCGGATC AGCTCTGCGG GGAACCTCTT CGTCAGCTTG 
TCCTACGGTG AGGGGTCAGA GATATCGGCT CCAATTTTTG TGGCAGCCCC GCGGAATATA 
GTAGTTGTGA AAGGAACAAA CATGACTCTC GACTGTGCTG CAAATGGAAA CCCTCGTCCG 
AACCTGACGT GGCTCAAGGA TGGTGTAACC ATCGACATGT CGCTTCTTGA TAGCAGGTTT 
AGAAAAGTCG GAGTAGGAAG TCTGCAAATT GAAAATATCC AGGAAGCGGA CGAAGGAACC 
TACATGTGCA GAGCAGAAAA TCACGAAGAC TCTGTGGATG CCAGTGCCAC CATTGAAATC 
CAAGTTCCTC CAAGGTTCAG AAAGAAACCG AAGAACAAGT TTGCATACGA GAAGGAAGAC 
GTTGAGTTTG AGTGCGAGGT GTACGGAAAG CCGGAGCCCT CGGTGCAGTG GTTCCGTAAC 
GGGGAAGCCA TCATCCAGAG TGAATACTTC CAAATCGTCA ACGGCAACAG CTTGAGGATA 
CTGGGTCTCG TGGACTCCGA CCGAGGAATG TACCAGTGCT TCGGCTCCAA CCCGGCCGGT 
AATGTCCAGA CATCGGCCCA ACTCGTGGTT CTCGACCCAG GGGAGCTGCA CAGGGGCAAG 
AACGTCCCCT CCGCCCCTCG GGAGGTCATG GCACGGCTCG TGTCCACTCG CTTTGCCACG 
CTCTCGTGGA CGGTGCCCGA GAAAGTGGAC GGAAGCATCC TCGCTTACTC CGTCTACTAC 
AAGGAGGAAG GCTCCAGCCG GTCAGTGGTG GTCCTTGAAC CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IscW_ISCW023403 ( XM_029980639.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029836499.1 Ixodes scapularis neogenin (LOC8041994), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIa23562
Clone ID Related Accession (Same CDS sequence)	XM_029980639.1
Accession Version	XM_029980639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	neogenin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919574.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGC TGCACCGCAA ACAACGTGGT TTACGATGGC TCATCCTGGC GATTTCCTGG 
GTCCTCGTCT CCGTCCCCGA CGTCGTGAAA GGTGATAACG TGTTCTTCCC GGACTTCCGC 
TTTCTGGAGG AGCCTTCTGA CATGGTGGCC ATCAAGGACA GCCCTGCGAT GCTCAACTGT 
TCGGCAGCCG GGGATCCCGA GCCCACCATT GAATGGAAGC GGGAAGGCTC GCTACTCCAG 
CTCGATGGCG ATCCAAGAAG GTCAATCCTC TCCAATGGCT CCCTCTTCTT GCGGAGTGTG 
CACCACACAA GAACAGAGAG ACCCGACGAG GGAGTCTACC AGTGCTTGGC CACCATTCCA 
AATGTTGGCA CCATCCTGAG TCGCTCTGCA AAGCTCCAAG TGGCTGCTCT GCCAAGGTTT 
GAAGAGCAGC CGAGGGACAT GCGGCTGTTC CCGGGGCAGA CGGCATACTT CCCCTGCTCG 
GCCTTTGCGG TCCCTTCGGC CAGCGTGGTG TGGCTCAAAG ACCAGCAGCC GCTGCAGCTT 
GACCCCTCCC GGATGCTGGT GTTGCCCAGC GGGGCCCTCG AGATTGACGG GGTGCAGACC 
ACCGACGAGG GGGCCTACCA GTGCACCGCC TACAATGCCG ACAAGAACCG GATCAGCTCT 
GCAGGGAACC TCTTCGTCAG CTTGTCCTAC GATGATGCCA ATAAGATATC GGCTCCAATT 
TTTGTGGCAG CCCCGCGGAA TATAGTAGTT GTGAAAGGAA CAAACATGAC TCTCGACTGT 
GCTGCAAATG GAAACCCTCG TCCGAACCTG ACGTGGCTCA AGGATGGTGT AACCATCGAC 
ATGTCGCTTC TTGATAGCAG GTTTAGAAAA GTCGGAGTAG GAAGTCTGCA AATTGAAAAT 
ATCCAGGAAG CGGACGAAGG AACCTACATG TGCAGAGCAG AAAATCACGA AGACTCTGTG 
GATGCCAGTG CCACCATTGA AATTCAAGTT CCTCCGAGGT TCAGAAAGAA ACCGAAGAAC 
AAGTTTGCAT ACGAGAAGGA AGACGTTGAG TTTGAGTGCG AGGTGTACGG AAAGCCGGAG 
CCCTCGGTGC AGTGGTTCCG TAACGGGGAA GCCATCATCC AGAGTGAATA CTTCCAAATC 
GTCAACGGCA ACAGCTTGAG GATACTGGGT CTCGTGGACT CGGACCGAGG AATGTACCAG 
TGCTTCGGCT CCAACCCGGC CGGTAATGTC CAGACATCGG CCCAACTCGT GGTTCTCGAC 
CCAGACTCTC CCCACCCTGG CACCCGTGCT TCCTTTACTA CTCCCTACCT GGAATACTCA 
GGGGAGCTGC ACAGGGGCAA GAACGTCCCC TCCGCCCCTC GGGAGGTCAT GGCACGGCTC 
GTGTCCACTC GCTTTGCCAC GCTCTCGTGG ACGGTGCCCG AGAAAGTGGA CGGAAGCATC 
CTCGCCTACT CCGTCTACTA CAAGGAGGAA GGCTCCAGCC GTGAGCGTGT GCTCAACACG 
ACGAGGCAAA GCTTGGAAGA GATCATCATC CCGGGTCTGA GGCCAGCCAC CAAGTATTTT 
TTTCGAGTTG TCGCCTACAA CGAACAGGGT CCGGGAAACA GTTCCCCAGA ACTGGAAGTG 
GAAACAAGCC CGGAAGTGAG CATCCCGGGG CCACCCCACA ACCTGAAGGC AGTGCCGACA 
TCCCCAACGG CCATCCGGGT GTTCTGGGAG GCCCCTGAGG GCCGCAGCAA GGAGGCAGCC 
CAGCACTACC AGCTCTCCTA CCAGGAGGCC GGCACCACGG AGGAGGAGGA GCGCAGGGTG 
ACCACCGCAG AAACCTGGTA CCACCTCAAG AACCTCAAGA AGTACACCGA GTACAACATC 
TGGATCACGG CCGTCAACCA GAACGGCTCC GGCATCAGCT CCGAGGAGAC CCTGGCACGC 
ACTTTCTCCG ATGCTCCCTC AGAAATTCCA CAAAACGTCA CCCTGGAAGC AGCAAGTTCT 
ACAAGCATAA TAATTAGGTG GGAGCCGCCT CCTAAGGAAT CGCAGAATGG AATAATTACT 
GGGTACAAGA TTAGGTACAA ATTGAAAGGA AGTCGGAGAG GGGACACCTT CACAACTGAC 
GGAAACAGAA GGCTTTTTGC TCTGTCGGGT CTTGAAAAAG GGGCGCAGTA CAGCGTCAAA 
ATTGCTGCCC TCTCGGTAAA CGGCACGGGT CCGGCCACGG ACTGGATGAC CGTGGAGACC 
TACCAGAATG ACCTAGATGA GTCTCGAGTG CCAGACCAGC CGAGCTCGCT GCGTGCCAAA 
CCGACCTCGA CCAGCATCTT TGTCAGCTGG GGTCCTCCCC GGAACCTGGA CATCATGATT 
CGGGGCTACA CGATTGGATG GGGCATTGGG CTGCCCGATG TTTACACCAA GGTCCTCAAT 
GGGAAGCAGC GTTACTACAC CATCGAAAAC TTGCAGCCTT CAAATGAGTA TGTCATCAGT 
GTGCGGGCAT TCAATCAAAT TGGTGATGGC CAGCCAATCT ATGAAACTGT CAAGACAACG 
ATTGAATCAA CTCCCGAGCC GTTGCTGCCC ATGCTGCCCC CTGTGGGGCT GAAGGCCATC 
GTGCTGTCGC CGACGACGGT GGTGCTGTTC TGGAGCGACA GCACGCTGTC GAGCAGCCAG 
GTGATCACAG ACAGCCGGCT GTACACAGTG CGCTACAAGA GCCAGGGCTC GCCCAAGTAC 
AAGTACTTCA ACTCGACCAA CCTGAACTGC ATGATCGACG ACCTGCGGCC CAACACCCAG 
TACGAGTTTG CCGTCAAGGT GGTCAAGGGC CGCAGGGAAA GCACCTACAG CATGGACGTG 
CTCAACACCA CCCAGGAGAG CGCACCCTCT TCTCCTCCAA GGGATCTGAC AATTGTGCCC 
AGCGATGACT CTCCAATGAT GATCAACCTT CACTGGCAAC CTCCCAAGCA GCAGAATGGA 
ATGATCACTG GCTACATGAT TTACTACACG ACGGACAACA GCCAGCAGGA CCGGGACTGG 
GCAGTAGAGG GCGTAGTGGG GGACAGGATG ACCACAGTCA TCAAAGGCCT CACCCCGGAC 
ACCACCTACT ACTTCAAGAT CCAGGCCAGG AACAAGATGG GCTATGGACC CCTCTCCCAG 
GAGGTCATGT ATCTCACGGG GAAAAGTTTA GAACTTGCCT CTGGAGGATC GAACAATGGT 
GGAAGCTTCA CAAACAATAC CCTGTACATT GCCATAACTT CTGGGTTTAC AGTGATCCTC 
CTAATACTAG TCATCATCTG CTACATGATG TGCCGCCGTC GTCAAGGCTA CATAGGACCG 
GTTAACAGTA AAGGGTATGC CAGCAGTAAG GGCATCGCCA AGGGCAAAGG AGCGACCAAG 
GACCTGAAGC CACCCGACCT TTGGATCCAC CACGATCAGA TGGAACTCAA GGCCATGGAC 
AAAAGCAGCA ACCCGGAGGC AGCCATGACT GTGACCCCCA TCTCGAGGAA CTCCCAGGAC 
CTGTCCGAGG ACCACATCGT GGGTACCTTG GAGAAGAAGA AGAACTCGGG CGGTTACATG 
GACCAGTTAA GCAGCGATGA CAGCGGCAAA ACAGATAGGC CCTTGTTGCC AAGGTCGTCT 
CGGTCTAAGC CTGTGATGTT GCCCCTTGAC TCTCGCAAGT CACTGCCTGA TTCCAGTAAA 
GCATCCATGG GACCGGCGGC CGTTTCCAAC GGCAACATGA ACCCTGGTTT AGAAGGGAGT 
TCGGTCAACA TGGGGCGGCC CATTTATCCC AGGACGCAGT ACAATATCCC CAGAGCCCAC 
GTGACCATTG ACTCACTGCA CCCAGGTCAC GACGGAATGA TGGCCAGCCC CCAGAAGCCG 
GGGGGCCTGA ACCCGACGTA TGAGCCCATC CTGAACCCGG GCATGTGCCA CGGGGGGGGT 
GCCATGGGCC AGCTGCCGGC CTACAGCAAT GCCCTGGTAG GGGGCCCCCC CGTGCCCCCC 
GGCCCGGGAC CCGGGGGCTC CGTGCCAGGC CCCGCAGAGT CCGGCACGCT CACCAAGAGA 
GCCCACTCCG GGAACCCCCT CAAAAGCTTC AGCGTGCCGG CCCCTCCCCC TCAGAGCGCC 
CCTTCCACGC CCCAACCCAA GCACATGATA GTGAGGCCTC AGCAAGTGTC CAGTCCCCAC 
AAGAAGGCAT CTGTTGCGAC ATCTACTTCT GCGGGGGCCG GGTCGTCGAA CAAGCAGAGC 
CCTGGGCGAC AGTCCGCACT CAAGTCACGA GCGGTCGCTT CGCCACGCAA CGTCTCCAAG 
GACTCCTCCA CTGCTCACAC TCCCAAGAAA GAGGAAGACC TTGCCTCTCC CTTCAACAAC 
GAAGAACTCA CCCAAGAAAT GGCCAACTTG GAAGGGCTGA TGAAGGACCT GAATGCAATA 
ACAGCATCGG AGTTTGAGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029836499.1
CDS	235..4638
Translation	MAGLHRKQRGLRWLILAISWVLVSVPDVVKGDNVFFPDFRFLEEPSDMVAIKDSPAMLNCSAAGDPEPTIEWKREGSLLQLDGDPRRSILSNGSLFLRSVHHTRTERPDEGVYQCLATIPNVGTILSRSAKLQVAALPRFEEQPRDMRLFPGQTAYFPCSAFAVPSASVVWLKDQQPLQLDPSRMLVLPSGALEIDGVQTTDEGAYQCTAYNADKNRISSAGNLFVSLSYDDANKISAPIFVAAPRNIVVVKGTNMTLDCAANGNPRPNLTWLKDGVTIDMSLLDSRFRKVGVGSLQIENIQEADEGTYMCRAENHEDSVDASATIEIQVPPRFRKKPKNKFAYEKEDVEFECEVYGKPEPSVQWFRNGEAIIQSEYFQIVNGNSLRILGLVDSDRGMYQCFGSNPAGNVQTSAQLVVLDPDSPHPGTRASFTTPYLEYSGELHRGKNVPSAPREVMARLVSTRFATLSWTVPEKVDGSILAYSVYYKEEGSSRERVLNTTRQSLEEIIIPGLRPATKYFFRVVAYNEQGPGNSSPELEVETSPEVSIPGPPHNLKAVPTSPTAIRVFWEAPEGRSKEAAQHYQLSYQEAGTTEEEERRVTTAETWYHLKNLKKYTEYNIWITAVNQNGSGISSEETLARTFSDAPSEIPQNVTLEAASSTSIIIRWEPPPKESQNGIITGYKIRYKLKGSRRGDTFTTDGNRRLFALSGLEKGAQYSVKIAALSVNGTGPATDWMTVETYQNDLDESRVPDQPSSLRAKPTSTSIFVSWGPPRNLDIMIRGYTIGWGIGLPDVYTKVLNGKQRYYTIENLQPSNEYVISVRAFNQIGDGQPIYETVKTTIESTPEPLLPMLPPVGLKAIVLSPTTVVLFWSDSTLSSSQVITDSRLYTVRYKSQGSPKYKYFNSTNLNCMIDDLRPNTQYEFAVKVVKGRRESTYSMDVLNTTQESAPSSPPRDLTIVPSDDSPMMINLHWQPPKQQNGMITGYMIYYTTDNSQQDRDWAVEGVVGDRMTTVIKGLTPDTTYYFKIQARNKMGYGPLSQEVMYLTGKSLELASGGSNNGGSFTNNTLYIAITSGFTVILLILVIICYMMCRRRQGYIGPVNSKGYASSKGIAKGKGATKDLKPPDLWIHHDQMELKAMDKSSNPEAAMTVTPISRNSQDLSEDHIVGTLEKKKNSGGYMDQLSSDDSGKTDRPLLPRSSRSKPVMLPLDSRKSLPDSSKASMGPAAVSNGNMNPGLEGSSVNMGRPIYPRTQYNIPRAHVTIDSLHPGHDGMMASPQKPGGLNPTYEPILNPGMCHGGGAMGQLPAYSNALVGGPPVPPGPGPGGSVPGPAESGTLTKRAHSGNPLKSFSVPAPPPQSAPSTPQPKHMIVRPQQVSSPHKKASVATSTSAGAGSSNKQSPGRQSALKSRAVASPRNVSKDSSTAHTPKKEEDLASPFNNEELTQEMANLEGLMKDLNAITASEFEC

Target ORF information:

RefSeq Version	XM_029980639.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis neogenin (LOC8041994), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029980639.1

ORF Insert Sequence:

ATGGCTGGGC TGCACCGCAA ACAACGTGGT TTACGATGGC TCATCCTGGC GATTTCCTGG 
GTCCTCGTCT CCGTCCCCGA CGTCGTGAAA GGTGATAACG TGTTCTTCCC GGACTTCCGC 
TTTCTGGAGG AGCCTTCTGA CATGGTGGCC ATCAAGGACA GCCCTGCGAT GCTCAACTGT 
TCGGCAGCCG GGGATCCCGA GCCCACCATT GAATGGAAGC GGGAAGGCTC GCTACTCCAG 
CTCGATGGCG ATCCAAGAAG GTCAATCCTC TCCAATGGCT CCCTCTTCTT GCGGAGTGTG 
CACCACACAA GAACAGAGAG ACCCGACGAG GGAGTCTACC AGTGCTTGGC CACCATTCCA 
AATGTTGGCA CCATCCTGAG TCGCTCTGCA AAGCTCCAAG TGGCTGCTCT GCCAAGGTTT 
GAAGAGCAGC CGAGGGACAT GCGGCTGTTC CCGGGGCAGA CGGCATACTT CCCCTGCTCG 
GCCTTTGCGG TCCCTTCGGC CAGCGTGGTG TGGCTCAAAG ACCAGCAGCC GCTGCAGCTT 
GACCCCTCCC GGATGCTGGT GTTGCCCAGC GGGGCCCTCG AGATTGACGG GGTGCAGACC 
ACCGACGAGG GGGCCTACCA GTGCACCGCC TACAATGCCG ACAAGAACCG GATCAGCTCT 
GCAGGGAACC TCTTCGTCAG CTTGTCCTAC GATGATGCCA ATAAGATATC GGCTCCAATT 
TTTGTGGCAG CCCCGCGGAA TATAGTAGTT GTGAAAGGAA CAAACATGAC TCTCGACTGT 
GCTGCAAATG GAAACCCTCG TCCGAACCTG ACGTGGCTCA AGGATGGTGT AACCATCGAC 
ATGTCGCTTC TTGATAGCAG GTTTAGAAAA GTCGGAGTAG GAAGTCTGCA AATTGAAAAT 
ATCCAGGAAG CGGACGAAGG AACCTACATG TGCAGAGCAG AAAATCACGA AGACTCTGTG 
GATGCCAGTG CCACCATTGA AATTCAAGTT CCTCCGAGGT TCAGAAAGAA ACCGAAGAAC 
AAGTTTGCAT ACGAGAAGGA AGACGTTGAG TTTGAGTGCG AGGTGTACGG AAAGCCGGAG 
CCCTCGGTGC AGTGGTTCCG TAACGGGGAA GCCATCATCC AGAGTGAATA CTTCCAAATC 
GTCAACGGCA ACAGCTTGAG GATACTGGGT CTCGTGGACT CGGACCGAGG AATGTACCAG 
TGCTTCGGCT CCAACCCGGC CGGTAATGTC CAGACATCGG CCCAACTCGT GGTTCTCGAC 
CCAGACTCTC CCCACCCTGG CACCCGTGCT TCCTTTACTA CTCCCTACCT GGAATACTCA 
GGGGAGCTGC ACAGGGGCAA GAACGTCCCC TCCGCCCCTC GGGAGGTCAT GGCACGGCTC 
GTGTCCACTC GCTTTGCCAC GCTCTCGTGG ACGGTGCCCG AGAAAGTGGA CGGAAGCATC 
CTCGCCTACT CCGTCTACTA CAAGGAGGAA GGCTCCAGCC GTGAGCGTGT GCTCAACACG 
ACGAGGCAAA GCTTGGAAGA GATCATCATC CCGGGTCTGA GGCCAGCCAC CAAGTATTTT 
TTTCGAGTTG TCGCCTACAA CGAACAGGGT CCGGGAAACA GTTCCCCAGA ACTGGAAGTG 
GAAACAAGCC CGGAAGTGAG CATCCCGGGG CCACCCCACA ACCTGAAGGC AGTGCCGACA 
TCCCCAACGG CCATCCGGGT GTTCTGGGAG GCCCCTGAGG GCCGCAGCAA GGAGGCAGCC 
CAGCACTACC AGCTCTCCTA CCAGGAGGCC GGCACCACGG AGGAGGAGGA GCGCAGGGTG 
ACCACCGCAG AAACCTGGTA CCACCTCAAG AACCTCAAGA AGTACACCGA GTACAACATC 
TGGATCACGG CCGTCAACCA GAACGGCTCC GGCATCAGCT CCGAGGAGAC CCTGGCACGC 
ACTTTCTCCG ATGCTCCCTC AGAAATTCCA CAAAACGTCA CCCTGGAAGC AGCAAGTTCT 
ACAAGCATAA TAATTAGGTG GGAGCCGCCT CCTAAGGAAT CGCAGAATGG AATAATTACT 
GGGTACAAGA TTAGGTACAA ATTGAAAGGA AGTCGGAGAG GGGACACCTT CACAACTGAC 
GGAAACAGAA GGCTTTTTGC TCTGTCGGGT CTTGAAAAAG GGGCGCAGTA CAGCGTCAAA 
ATTGCTGCCC TCTCGGTAAA CGGCACGGGT CCGGCCACGG ACTGGATGAC CGTGGAGACC 
TACCAGAATG ACCTAGATGA GTCTCGAGTG CCAGACCAGC CGAGCTCGCT GCGTGCCAAA 
CCGACCTCGA CCAGCATCTT TGTCAGCTGG GGTCCTCCCC GGAACCTGGA CATCATGATT 
CGGGGCTACA CGATTGGATG GGGCATTGGG CTGCCCGATG TTTACACCAA GGTCCTCAAT 
GGGAAGCAGC GTTACTACAC CATCGAAAAC TTGCAGCCTT CAAATGAGTA TGTCATCAGT 
GTGCGGGCAT TCAATCAAAT TGGTGATGGC CAGCCAATCT ATGAAACTGT CAAGACAACG 
ATTGAATCAA CTCCCGAGCC GTTGCTGCCC ATGCTGCCCC CTGTGGGGCT GAAGGCCATC 
GTGCTGTCGC CGACGACGGT GGTGCTGTTC TGGAGCGACA GCACGCTGTC GAGCAGCCAG 
GTGATCACAG ACAGCCGGCT GTACACAGTG CGCTACAAGA GCCAGGGCTC GCCCAAGTAC 
AAGTACTTCA ACTCGACCAA CCTGAACTGC ATGATCGACG ACCTGCGGCC CAACACCCAG 
TACGAGTTTG CCGTCAAGGT GGTCAAGGGC CGCAGGGAAA GCACCTACAG CATGGACGTG 
CTCAACACCA CCCAGGAGAG CGCACCCTCT TCTCCTCCAA GGGATCTGAC AATTGTGCCC 
AGCGATGACT CTCCAATGAT GATCAACCTT CACTGGCAAC CTCCCAAGCA GCAGAATGGA 
ATGATCACTG GCTACATGAT TTACTACACG ACGGACAACA GCCAGCAGGA CCGGGACTGG 
GCAGTAGAGG GCGTAGTGGG GGACAGGATG ACCACAGTCA TCAAAGGCCT CACCCCGGAC 
ACCACCTACT ACTTCAAGAT CCAGGCCAGG AACAAGATGG GCTATGGACC CCTCTCCCAG 
GAGGTCATGT ATCTCACGGG GAAAAGTTTA GAACTTGCCT CTGGAGGATC GAACAATGGT 
GGAAGCTTCA CAAACAATAC CCTGTACATT GCCATAACTT CTGGGTTTAC AGTGATCCTC 
CTAATACTAG TCATCATCTG CTACATGATG TGCCGCCGTC GTCAAGGCTA CATAGGACCG 
GTTAACAGTA AAGGGTATGC CAGCAGTAAG GGCATCGCCA AGGGCAAAGG AGCGACCAAG 
GACCTGAAGC CACCCGACCT TTGGATCCAC CACGATCAGA TGGAACTCAA GGCCATGGAC 
AAAAGCAGCA ACCCGGAGGC AGCCATGACT GTGACCCCCA TCTCGAGGAA CTCCCAGGAC 
CTGTCCGAGG ACCACATCGT GGGTACCTTG GAGAAGAAGA AGAACTCGGG CGGTTACATG 
GACCAGTTAA GCAGCGATGA CAGCGGCAAA ACAGATAGGC CCTTGTTGCC AAGGTCGTCT 
CGGTCTAAGC CTGTGATGTT GCCCCTTGAC TCTCGCAAGT CACTGCCTGA TTCCAGTAAA 
GCATCCATGG GACCGGCGGC CGTTTCCAAC GGCAACATGA ACCCTGGTTT AGAAGGGAGT 
TCGGTCAACA TGGGGCGGCC CATTTATCCC AGGACGCAGT ACAATATCCC CAGAGCCCAC 
GTGACCATTG ACTCACTGCA CCCAGGTCAC GACGGAATGA TGGCCAGCCC CCAGAAGCCG 
GGGGGCCTGA ACCCGACGTA TGAGCCCATC CTGAACCCGG GCATGTGCCA CGGGGGGGGT 
GCCATGGGCC AGCTGCCGGC CTACAGCAAT GCCCTGGTAG GGGGCCCCCC CGTGCCCCCC 
GGCCCGGGAC CCGGGGGCTC CGTGCCAGGC CCCGCAGAGT CCGGCACGCT CACCAAGAGA 
GCCCACTCCG GGAACCCCCT CAAAAGCTTC AGCGTGCCGG CCCCTCCCCC TCAGAGCGCC 
CCTTCCACGC CCCAACCCAA GCACATGATA GTGAGGCCTC AGCAAGTGTC CAGTCCCCAC 
AAGAAGGCAT CTGTTGCGAC ATCTACTTCT GCGGGGGCCG GGTCGTCGAA CAAGCAGAGC 
CCTGGGCGAC AGTCCGCACT CAAGTCACGA GCGGTCGCTT CGCCACGCAA CGTCTCCAAG 
GACTCCTCCA CTGCTCACAC TCCCAAGAAA GAGGAAGACC TTGCCTCTCC CTTCAACAAC 
GAAGAACTCA CCCAAGAAAT GGCCAACTTG GAAGGGCTGA TGAAGGACCT GAATGCAATA 
ACAGCATCGG AGTTTGAGTG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IscW_ISCW023403 ( XM_029980640.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029836500.1 Ixodes scapularis neogenin (LOC8041994), transcript variant X2, mRNA.

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CloneID	OIa23563
Clone ID Related Accession (Same CDS sequence)	XM_029980640.1
Accession Version	XM_029980640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	neogenin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919574.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGC TGCACCGCAA ACAACGTGGT TTACGATGGC TCATCCTGGC GATTTCCTGG 
GTCCTCGTCT CCGTCCCCGA CGTCGTGAAA GGTGATAACG TGTTCTTCCC GGACTTCCGC 
TTTCTGGAGG AGCCTTCTGA CATGGTGGCC ATCAAGGACA GCCCTGCGAT GCTCAACTGT 
TCGGCAGCCG GGGATCCCGA GCCCACCATT GAATGGAAGC GGGAAGGCTC GCTACTCCAG 
CTCGATGGCG ATCCAAGAAG GTCAATCCTC TCCAATGGCT CCCTCTTCTT GCGGAGTGTG 
CACCACACAA GAACAGAGAG ACCCGACGAG GGAGTCTACC AGTGCTTGGC CACCATTCCA 
AATGTTGGCA CCATCCTGAG TCGCTCTGCA AAGCTCCAAG TGGCTGCTCT GCCAAGGTTT 
GAAGAGCAGC CGAGGGACAT GCGGCTGTTC CCGGGGCAGA CGGCATACTT CCCCTGCTCG 
GCCTTTGCGG TCCCTTCGGC CAGCGTGGTG TGGCTCAAAG ACCAGCAGCC GCTGCAGCTT 
GACCCCTCCC GGATGCTGGT GTTGCCCAGC GGGGCCCTCG AGATTGACGG GGTGCAGACC 
ACCGACGAGG GGGCCTACCA GTGCACCGCC TACAATGCCG ACAAGAACCG GATCAGCTCT 
GCAGGGAACC TCTTCGTCAG CTTGTCCTAC GATGATGCCA ATAAGATATC GGCTCCAATT 
TTTGTGGCAG CCCCGCGGAA TATAGTAGTT GTGAAAGGAA CAAACATGAC TCTCGACTGT 
GCTGCAAATG GAAACCCTCG TCCGAACCTG ACGTGGCTCA AGGATGGTGT AACCATCGAC 
ATGTCGCTTC TTGATAGCAG GTTTAGAAAA GTCGGAGTAG GAAGTCTGCA AATTGAAAAT 
ATCCAGGAAG CGGACGAAGG AACCTACATG TGCAGAGCAG AAAATCACGA AGACTCTGTG 
GATGCCAGTG CCACCATTGA AATTCAAGTT CCTCCGAGGT TCAGAAAGAA ACCGAAGAAC 
AAGTTTGCAT ACGAGAAGGA AGACGTTGAG TTTGAGTGCG AGGTGTACGG AAAGCCGGAG 
CCCTCGGTGC AGTGGTTCCG TAACGGGGAA GCCATCATCC AGAGTGAATA CTTCCAAATC 
GTCAACGGCA ACAGCTTGAG GATACTGGGT CTCGTGGACT CGGACCGAGG AATGTACCAG 
TGCTTCGGCT CCAACCCGGC CGGTAATGTC CAGACATCGG CCCAACTCGT GGTTCTCGAC 
CCAGACTCTC CCCACCCTGG CACCCGTGCT TCCTTTACTA CTCCCTACCT GGAATACTCA 
GGGGAGCTGC ACAGGGGCAA GAACGTCCCC TCCGCCCCTC GGGAGGTCAT GGCACGGCTC 
GTGTCCACTC GCTTTGCCAC GCTCTCGTGG ACGGTGCCCG AGAAAGTGGA CGGAAGCATC 
CTCGCCTACT CCGTCTACTA CAAGGAGGAA GGCTCCAGCC GTGAGCGTGT GCTCAACACG 
ACGAGGCAAA GCTTGGAAGA GATCATCATC CCGGGTCTGA GGCCAGCCAC CAAGTATTTT 
TTTCGAGTTG TCGCCTACAA CGAACAGGGT CCGGGAAACA GTTCCCCAGA ACTGGAAGTG 
GAAACAAGCC CGGAAGTGAG CATCCCGGGG CCACCCCACA ACCTGAAGGC AGTGCCGACA 
TCCCCAACGG CCATCCGGGT GTTCTGGGAG GCCCCTGAGG GCCGCAGCAA GGAGGCAGCC 
CAGCACTACC AGCTCTCCTA CCAGGAGGCC GGCACCACGG AGGAGGAGGA GCGCAGGGTG 
ACCACCGCAG AAACCTGGTA CCACCTCAAG AACCTCAAGA AGTACACCGA GTACAACATC 
TGGATCACGG CCGTCAACCA GAACGGCTCC GGCATCAGCT CCGAGGAGAC CCTGGCACGC 
ACTTTCTCCG ATGCTCCCTC AGAAATTCCA CAAAACGTCA CCCTGGAAGC AGCAAGTTCT 
ACAAGCATAA TAATTAGGTG GGAGCCGCCT CCTAAGGAAT CGCAGAATGG AATAATTACT 
GGGTACAAGA TTAGGTACAA ATTGAAAGGA AGTCGGAGAG GGGACACCTT CACAACTGAC 
GGAAACAGAA GGCTTTTTGC TCTGTCGGGT CTTGAAAAAG GGGCGCAGTA CAGCGTCAAA 
ATTGCTGCCC TCTCGGTAAA CGGCACGGGT CCGGCCACGG ACTGGATGAC CGTGGAGACC 
TACCAGAATG ACCTAGATGA GTCTCGAGTG CCAGACCAGC CGAGCTCGCT GCGTGCCAAA 
CCGACCTCGA CCAGCATCTT TGTCAGCTGG GGTCCTCCCC GGAACCTGGA CATCATGATT 
CGGGGCTACA CGATTGGATG GGGCATTGGG CTGCCCGATG TTTACACCAA GGTCCTCAAT 
GGGAAGCAGC GTTACTACAC CATCGAAAAC TTGCAGCCTT CAAATGAGTA TGTCATCAGT 
GTGCGGGCAT TCAATCAAAT TGGTGATGGC CAGCCAATCT ATGAAACTGT CAAGACAACG 
ATTGAATCAA CTCCCGAGCC GTTGCTGCCC ATGCTGCCCC CTGTGGGGCT GAAGGCCATC 
GTGCTGTCGC CGACGACGGT GGTGCTGTTC TGGAGCGACA GCACGCTGTC GAGCAGCCAG 
GTGATCACAG ACAGCCGGCT GTACACAGTG CGCTACAAGA GCCAGGGCTC GCCCAAGTAC 
AAGTACTTCA ACTCGACCAA CCTGAACTGC ATGATCGACG ACCTGCGGCC CAACACCCAG 
TACGAGTTTG CCGTCAAGGT GGTCAAGGGC CGCAGGGAAA GCACCTACAG CATGGACGTG 
CTCAACACCA CCCAGGAGAG CGCACCCTCT TCTCCTCCAA GGGATCTGAC AATTGTGCCC 
AGCGATGACT CTCCAATGAT GATCAACCTT CACTGGCAAC CTCCCAAGCA GCAGAATGGA 
ATGATCACTG GCTACATGAT TTACTACACG ACGGACAACA GCCAGCAGGA CCGGGACTGG 
GCAGTAGAGG GCGTAGTGGG GGACAGGATG ACCACAGTCA TCAAAGGCCT CACCCCGGAC 
ACCACCTACT ACTTCAAGAT CCAGGCCAGG AACAAGATGG GCTATGGACC CCTCTCCCAG 
GAGGTCATGT ATCTCACGGG GAAAAGTTTA GAACTTGCCT CTGGAGGATC GAACAATGGT 
GGAAGCTTCA CAAACAATAC CCTGTACATT GCCATAACTT CTGGGTTTAC AGTGATCCTC 
CTAATACTAG TCATCATCTG CTACATGATG TGCCGCCGTC GTCAAGGCTA CATAGGACCG 
GTTAACAGTA AAGGGTATGC CAGCAGTAAG GGCATCGCCA AGGGCAAAGG AGCGACCAAG 
GACCTGAAGC CACCCGACCT TTGGATCCAC CACGATCAGA TGGAACTCAA GGCCATGGAC 
AAAAGCAGCA ACCCGGAGGC AGCCATGACT GTGACCCCCA TCTCGAGGAA CTCCCAGGAC 
CTGTCCGAGG ACCACATCGT GGGTACCTTG GAGAAGAAGA AGAACTCGGG CGGTTACATG 
GACCAGTTAA GCAGCGATGA CAGCGGCAAA ACAGATAGGC CCTTGTTGCC AAGGTCGTCT 
CGGTCTAAGC CTGTGATGTT GCCCCTTGAC TCTCGCAAGT CACTGCCTGA TTCCAGTAAA 
GCATCCATGG GACCGGCGGC CGTTTCCAAC GGCAACATGA ACCCTGGTTT AGAAGGGAGT 
TCGGTCAACA TGGGGCGGCC CATTTATCCC AGGACGCAGT ACAATATCCC CAGAGCCCAC 
GTGACCATTG ACTCACTGCA CCCAGGTCAC GACGGAATGA TGGCCAGCCC CCAGAAGCCG 
GGGGGCCTGA ACCCGACGTA TGAGCCCATC CTGAACCCGG GCATGTGCCA CGGGGGGGGT 
GCCATGGGCC AGCTGCCGGC CTACAGCAAT GCCCTGGTAG GGGGCCCCCC CGTGCCCCCC 
GGCCCGGGAC CCGGGGGCTC CGTGCCAGGC CCCGCAGAGT CCGGCACGCT CACCAAGAGA 
GCCCACTCCG GGAACCCCCT CAAAAGCTTC AGCGTGCCGG CCCCTCCCCC TCAGAGCGCC 
CCTTCCACGC CCCAACCCAA GCACATGATG AGGCCTCAGC AAGTGTCCAG TCCCCACAAG 
AAGGCATCTG TTGCGACATC TACTTCTGCG GGGGCCGGGT CGTCGAACAA GCAGAGCCCT 
GGGCGACAGT CCGCACTCAA GTCACGAGCG GTCGCTTCGC CACGCAACGT CTCCAAGGAC 
TCCTCCACTG CTCACACTCC CAAGAAAGAG GAAGACCTTG CCTCTCCCTT CAACAACGAA 
GAACTCACCC AAGAAATGGC CAACTTGGAA GGGCTGATGA AGGACCTGAA TGCAATAACA 
GCATCGGAGT TTGAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029836500.1
CDS	235..4635
Translation	MAGLHRKQRGLRWLILAISWVLVSVPDVVKGDNVFFPDFRFLEEPSDMVAIKDSPAMLNCSAAGDPEPTIEWKREGSLLQLDGDPRRSILSNGSLFLRSVHHTRTERPDEGVYQCLATIPNVGTILSRSAKLQVAALPRFEEQPRDMRLFPGQTAYFPCSAFAVPSASVVWLKDQQPLQLDPSRMLVLPSGALEIDGVQTTDEGAYQCTAYNADKNRISSAGNLFVSLSYDDANKISAPIFVAAPRNIVVVKGTNMTLDCAANGNPRPNLTWLKDGVTIDMSLLDSRFRKVGVGSLQIENIQEADEGTYMCRAENHEDSVDASATIEIQVPPRFRKKPKNKFAYEKEDVEFECEVYGKPEPSVQWFRNGEAIIQSEYFQIVNGNSLRILGLVDSDRGMYQCFGSNPAGNVQTSAQLVVLDPDSPHPGTRASFTTPYLEYSGELHRGKNVPSAPREVMARLVSTRFATLSWTVPEKVDGSILAYSVYYKEEGSSRERVLNTTRQSLEEIIIPGLRPATKYFFRVVAYNEQGPGNSSPELEVETSPEVSIPGPPHNLKAVPTSPTAIRVFWEAPEGRSKEAAQHYQLSYQEAGTTEEEERRVTTAETWYHLKNLKKYTEYNIWITAVNQNGSGISSEETLARTFSDAPSEIPQNVTLEAASSTSIIIRWEPPPKESQNGIITGYKIRYKLKGSRRGDTFTTDGNRRLFALSGLEKGAQYSVKIAALSVNGTGPATDWMTVETYQNDLDESRVPDQPSSLRAKPTSTSIFVSWGPPRNLDIMIRGYTIGWGIGLPDVYTKVLNGKQRYYTIENLQPSNEYVISVRAFNQIGDGQPIYETVKTTIESTPEPLLPMLPPVGLKAIVLSPTTVVLFWSDSTLSSSQVITDSRLYTVRYKSQGSPKYKYFNSTNLNCMIDDLRPNTQYEFAVKVVKGRRESTYSMDVLNTTQESAPSSPPRDLTIVPSDDSPMMINLHWQPPKQQNGMITGYMIYYTTDNSQQDRDWAVEGVVGDRMTTVIKGLTPDTTYYFKIQARNKMGYGPLSQEVMYLTGKSLELASGGSNNGGSFTNNTLYIAITSGFTVILLILVIICYMMCRRRQGYIGPVNSKGYASSKGIAKGKGATKDLKPPDLWIHHDQMELKAMDKSSNPEAAMTVTPISRNSQDLSEDHIVGTLEKKKNSGGYMDQLSSDDSGKTDRPLLPRSSRSKPVMLPLDSRKSLPDSSKASMGPAAVSNGNMNPGLEGSSVNMGRPIYPRTQYNIPRAHVTIDSLHPGHDGMMASPQKPGGLNPTYEPILNPGMCHGGGAMGQLPAYSNALVGGPPVPPGPGPGGSVPGPAESGTLTKRAHSGNPLKSFSVPAPPPQSAPSTPQPKHMMRPQQVSSPHKKASVATSTSAGAGSSNKQSPGRQSALKSRAVASPRNVSKDSSTAHTPKKEEDLASPFNNEELTQEMANLEGLMKDLNAITASEFEC

Target ORF information:

RefSeq Version	XM_029980640.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis neogenin (LOC8041994), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029980640.1

ORF Insert Sequence:

ATGGCTGGGC TGCACCGCAA ACAACGTGGT TTACGATGGC TCATCCTGGC GATTTCCTGG 
GTCCTCGTCT CCGTCCCCGA CGTCGTGAAA GGTGATAACG TGTTCTTCCC GGACTTCCGC 
TTTCTGGAGG AGCCTTCTGA CATGGTGGCC ATCAAGGACA GCCCTGCGAT GCTCAACTGT 
TCGGCAGCCG GGGATCCCGA GCCCACCATT GAATGGAAGC GGGAAGGCTC GCTACTCCAG 
CTCGATGGCG ATCCAAGAAG GTCAATCCTC TCCAATGGCT CCCTCTTCTT GCGGAGTGTG 
CACCACACAA GAACAGAGAG ACCCGACGAG GGAGTCTACC AGTGCTTGGC CACCATTCCA 
AATGTTGGCA CCATCCTGAG TCGCTCTGCA AAGCTCCAAG TGGCTGCTCT GCCAAGGTTT 
GAAGAGCAGC CGAGGGACAT GCGGCTGTTC CCGGGGCAGA CGGCATACTT CCCCTGCTCG 
GCCTTTGCGG TCCCTTCGGC CAGCGTGGTG TGGCTCAAAG ACCAGCAGCC GCTGCAGCTT 
GACCCCTCCC GGATGCTGGT GTTGCCCAGC GGGGCCCTCG AGATTGACGG GGTGCAGACC 
ACCGACGAGG GGGCCTACCA GTGCACCGCC TACAATGCCG ACAAGAACCG GATCAGCTCT 
GCAGGGAACC TCTTCGTCAG CTTGTCCTAC GATGATGCCA ATAAGATATC GGCTCCAATT 
TTTGTGGCAG CCCCGCGGAA TATAGTAGTT GTGAAAGGAA CAAACATGAC TCTCGACTGT 
GCTGCAAATG GAAACCCTCG TCCGAACCTG ACGTGGCTCA AGGATGGTGT AACCATCGAC 
ATGTCGCTTC TTGATAGCAG GTTTAGAAAA GTCGGAGTAG GAAGTCTGCA AATTGAAAAT 
ATCCAGGAAG CGGACGAAGG AACCTACATG TGCAGAGCAG AAAATCACGA AGACTCTGTG 
GATGCCAGTG CCACCATTGA AATTCAAGTT CCTCCGAGGT TCAGAAAGAA ACCGAAGAAC 
AAGTTTGCAT ACGAGAAGGA AGACGTTGAG TTTGAGTGCG AGGTGTACGG AAAGCCGGAG 
CCCTCGGTGC AGTGGTTCCG TAACGGGGAA GCCATCATCC AGAGTGAATA CTTCCAAATC 
GTCAACGGCA ACAGCTTGAG GATACTGGGT CTCGTGGACT CGGACCGAGG AATGTACCAG 
TGCTTCGGCT CCAACCCGGC CGGTAATGTC CAGACATCGG CCCAACTCGT GGTTCTCGAC 
CCAGACTCTC CCCACCCTGG CACCCGTGCT TCCTTTACTA CTCCCTACCT GGAATACTCA 
GGGGAGCTGC ACAGGGGCAA GAACGTCCCC TCCGCCCCTC GGGAGGTCAT GGCACGGCTC 
GTGTCCACTC GCTTTGCCAC GCTCTCGTGG ACGGTGCCCG AGAAAGTGGA CGGAAGCATC 
CTCGCCTACT CCGTCTACTA CAAGGAGGAA GGCTCCAGCC GTGAGCGTGT GCTCAACACG 
ACGAGGCAAA GCTTGGAAGA GATCATCATC CCGGGTCTGA GGCCAGCCAC CAAGTATTTT 
TTTCGAGTTG TCGCCTACAA CGAACAGGGT CCGGGAAACA GTTCCCCAGA ACTGGAAGTG 
GAAACAAGCC CGGAAGTGAG CATCCCGGGG CCACCCCACA ACCTGAAGGC AGTGCCGACA 
TCCCCAACGG CCATCCGGGT GTTCTGGGAG GCCCCTGAGG GCCGCAGCAA GGAGGCAGCC 
CAGCACTACC AGCTCTCCTA CCAGGAGGCC GGCACCACGG AGGAGGAGGA GCGCAGGGTG 
ACCACCGCAG AAACCTGGTA CCACCTCAAG AACCTCAAGA AGTACACCGA GTACAACATC 
TGGATCACGG CCGTCAACCA GAACGGCTCC GGCATCAGCT CCGAGGAGAC CCTGGCACGC 
ACTTTCTCCG ATGCTCCCTC AGAAATTCCA CAAAACGTCA CCCTGGAAGC AGCAAGTTCT 
ACAAGCATAA TAATTAGGTG GGAGCCGCCT CCTAAGGAAT CGCAGAATGG AATAATTACT 
GGGTACAAGA TTAGGTACAA ATTGAAAGGA AGTCGGAGAG GGGACACCTT CACAACTGAC 
GGAAACAGAA GGCTTTTTGC TCTGTCGGGT CTTGAAAAAG GGGCGCAGTA CAGCGTCAAA 
ATTGCTGCCC TCTCGGTAAA CGGCACGGGT CCGGCCACGG ACTGGATGAC CGTGGAGACC 
TACCAGAATG ACCTAGATGA GTCTCGAGTG CCAGACCAGC CGAGCTCGCT GCGTGCCAAA 
CCGACCTCGA CCAGCATCTT TGTCAGCTGG GGTCCTCCCC GGAACCTGGA CATCATGATT 
CGGGGCTACA CGATTGGATG GGGCATTGGG CTGCCCGATG TTTACACCAA GGTCCTCAAT 
GGGAAGCAGC GTTACTACAC CATCGAAAAC TTGCAGCCTT CAAATGAGTA TGTCATCAGT 
GTGCGGGCAT TCAATCAAAT TGGTGATGGC CAGCCAATCT ATGAAACTGT CAAGACAACG 
ATTGAATCAA CTCCCGAGCC GTTGCTGCCC ATGCTGCCCC CTGTGGGGCT GAAGGCCATC 
GTGCTGTCGC CGACGACGGT GGTGCTGTTC TGGAGCGACA GCACGCTGTC GAGCAGCCAG 
GTGATCACAG ACAGCCGGCT GTACACAGTG CGCTACAAGA GCCAGGGCTC GCCCAAGTAC 
AAGTACTTCA ACTCGACCAA CCTGAACTGC ATGATCGACG ACCTGCGGCC CAACACCCAG 
TACGAGTTTG CCGTCAAGGT GGTCAAGGGC CGCAGGGAAA GCACCTACAG CATGGACGTG 
CTCAACACCA CCCAGGAGAG CGCACCCTCT TCTCCTCCAA GGGATCTGAC AATTGTGCCC 
AGCGATGACT CTCCAATGAT GATCAACCTT CACTGGCAAC CTCCCAAGCA GCAGAATGGA 
ATGATCACTG GCTACATGAT TTACTACACG ACGGACAACA GCCAGCAGGA CCGGGACTGG 
GCAGTAGAGG GCGTAGTGGG GGACAGGATG ACCACAGTCA TCAAAGGCCT CACCCCGGAC 
ACCACCTACT ACTTCAAGAT CCAGGCCAGG AACAAGATGG GCTATGGACC CCTCTCCCAG 
GAGGTCATGT ATCTCACGGG GAAAAGTTTA GAACTTGCCT CTGGAGGATC GAACAATGGT 
GGAAGCTTCA CAAACAATAC CCTGTACATT GCCATAACTT CTGGGTTTAC AGTGATCCTC 
CTAATACTAG TCATCATCTG CTACATGATG TGCCGCCGTC GTCAAGGCTA CATAGGACCG 
GTTAACAGTA AAGGGTATGC CAGCAGTAAG GGCATCGCCA AGGGCAAAGG AGCGACCAAG 
GACCTGAAGC CACCCGACCT TTGGATCCAC CACGATCAGA TGGAACTCAA GGCCATGGAC 
AAAAGCAGCA ACCCGGAGGC AGCCATGACT GTGACCCCCA TCTCGAGGAA CTCCCAGGAC 
CTGTCCGAGG ACCACATCGT GGGTACCTTG GAGAAGAAGA AGAACTCGGG CGGTTACATG 
GACCAGTTAA GCAGCGATGA CAGCGGCAAA ACAGATAGGC CCTTGTTGCC AAGGTCGTCT 
CGGTCTAAGC CTGTGATGTT GCCCCTTGAC TCTCGCAAGT CACTGCCTGA TTCCAGTAAA 
GCATCCATGG GACCGGCGGC CGTTTCCAAC GGCAACATGA ACCCTGGTTT AGAAGGGAGT 
TCGGTCAACA TGGGGCGGCC CATTTATCCC AGGACGCAGT ACAATATCCC CAGAGCCCAC 
GTGACCATTG ACTCACTGCA CCCAGGTCAC GACGGAATGA TGGCCAGCCC CCAGAAGCCG 
GGGGGCCTGA ACCCGACGTA TGAGCCCATC CTGAACCCGG GCATGTGCCA CGGGGGGGGT 
GCCATGGGCC AGCTGCCGGC CTACAGCAAT GCCCTGGTAG GGGGCCCCCC CGTGCCCCCC 
GGCCCGGGAC CCGGGGGCTC CGTGCCAGGC CCCGCAGAGT CCGGCACGCT CACCAAGAGA 
GCCCACTCCG GGAACCCCCT CAAAAGCTTC AGCGTGCCGG CCCCTCCCCC TCAGAGCGCC 
CCTTCCACGC CCCAACCCAA GCACATGATG AGGCCTCAGC AAGTGTCCAG TCCCCACAAG 
AAGGCATCTG TTGCGACATC TACTTCTGCG GGGGCCGGGT CGTCGAACAA GCAGAGCCCT 
GGGCGACAGT CCGCACTCAA GTCACGAGCG GTCGCTTCGC CACGCAACGT CTCCAAGGAC 
TCCTCCACTG CTCACACTCC CAAGAAAGAG GAAGACCTTG CCTCTCCCTT CAACAACGAA 
GAACTCACCC AAGAAATGGC CAACTTGGAA GGGCTGATGA AGGACCTGAA TGCAATAACA 
GCATCGGAGT TTGAGTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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IscW_ISCW023403 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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