LOC8038480 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8038480
Entrez Gene ID	8038480
Full Name	ubiquitination factor E4a, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002412648.1	XP_002412693.1	ubiquitination factor E4a, putative
XM_029978626.1	XP_029834486.1	ubiquitin conjugation factor E4 A
XM_029978627.1	XP_029834487.1	ubiquitin conjugation factor E4 A

Pathways from BioSystems
Gene Ontology

The following LOC8038480 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8038480 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa08724	XM_002412648.1 Latest version!	Ixodes scapularis ubiquitination factor E4a, putative, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OIa21841	XM_029978627.1 Latest version!	Ixodes scapularis ubiquitin conjugation factor E4 A (LOC8038480), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OIa21841	XM_029978626.1 Latest version!	Ixodes scapularis ubiquitin conjugation factor E4 A (LOC8038480), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8038480 ( XM_002412648.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002412693.1 Ixodes scapularis ubiquitination factor E4a, putative, mRNA.

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CloneID	OIa08724
Clone ID Related Accession (Same CDS sequence)	XM_002412648.1
Accession Version	XM_002412648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1245600000000
Organism	Ixodes scapularis(black-legged tick)
Product	ubiquitination factor E4a, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002790222). COMPLETENESS: incomplete on both ends.

ATGCAGCTGG TCTTCGAGCG GCTTGTGTTG GACGACCCCC AGAACCACGT GGTCAGCAAG 
AGCAAGTATG CCGACCAGCA GGCTCTGGCT GATGCCGGAG AAACCAGAGT GGTCTTCTAC 
CTCTACCAGT GCTTCTCGAG GCTCAACAAG GTGGCCGCCA AGCTGGAGGT ACCAGATCCC 
GAGCATCAGT CCGCTTCCCT CACGATTGTG TCGCAGGCGT CGACTTGCCT TTGCCACTGT 
GAAATCTACC CTGGCAAAAA GATACCGCAG CAGGTCTTCT ACCTACTCCA GGAGTATCAC 
GCTGAAGGAG GTCACCAAGA CAGCCTGGAG TCTTTTCTGA AGAAATGCGC AGACTGTATC 
AAGGGCCGCT TTGATGCAGA GGAAGAGCAG GTTTCTCTGC TTGAAGTGCT CTCGCATTCC 
TTCAACTTGC TCAAGAAGAA ATTCGGCAAG GTGTCCATCG CTAACCGGGA ACTGTTTGTG 
CTTATCGACA TCCTCAAGTA CTTCACCAAA TCTGTTTCCA TGGCAATGGC ACTCTTAGAC 
TTCAGCACGC CTGCAGAGCT GGTTGACCTA CGCAGTTTCC AGGAGAGCCT GCTCGGTGCC 
CCCCTGTGCG TGTCCTGCCT GCCACGCAAG GAAGCCGACC CCTTCGAGTT CTTCGAGAAG 
CCCTCTCAGG CGCTGATGAA CGAGCATGCC ATCACCGAGA GCAATCTGTG GCTGCCGCTA 
AAAGGTCTCG CAGTGGAGCT GCACAACCTG TTTTATTCCC TGCTTCGGCT TTCACCAGAG 
ACACGCAACA GGACCCTGAC ATGGATAGGA CACTGCATCA CTGCATGTTC AGAGCGGGGC 
AAACTGTGGA ACAACCAGGT GTCTGAGCTG TTCATGGCGG TGCAATCCGG CGACGGCTTT 
GCCCTGAACC TGGGGGCGGT GCTGCTGCGG CTGGCGCGGC CCTTCTCGGA GCCCTACTCT 
CCCAAGCTGC TCAAGGTACA GCACCAGTAC TGCTCCTTCG AGCCAAAGTC GGAGCAGGAA 
GCCAATGCCA GTTGCATGCA CATCAGAGGG CTGTCCAAGG AGACGTGCCT GGTGCCGAGG 
GAGGAGGGTG CGGCCAGCGA GTCCCCTTGC GGCCCCTTCA ACTTCTCGAC GGAGTGCTTC 
TTTGCCTGCC AACGCATCCT GAGCCTGGGC TTCCGGGTGG TGCACGAACG GCTGGCCCGG 
CTCAGCCAGG ACCTGAACCG GGTGCGCAGG GTCTACGAGG AGGCACGCTC GCAGGGCGGC 
GACAGCTCTG AGGTCACCCG CAGGCTGCAG GACAATATGG AGAAGGGCAT GACCCGGTTC 
CTGTCCCTGA AGGCGGTGCT CTTGGAGCCG GACTCCCTGG AGCACATGCT GCAGTTCCAC 
GTGGCCTGCG CCACCTGGCT GTGCCACCTG GCCACGGCCG AGAGGCTGGA CGCCTTCCAG 
CCCCTGTGCC TGCCTTTCCC CGAGCACGGC AACGCCAGGC TCGCCTATGT TCCGGAGTTT 
GTCATTGAGA ACATCTGTGA CTGCATTGTG TTTGTCAAGC GCTTCAGCGA AAAAAGTCTG 
GCGCTCGTGG GCCCGAACTT GGAGCACCTG ATGACGCTGA TACTGCTGTT CATGGGCAGC 
CCCCAGAGAA TGAACAACCC GCACCTAAGA GCCAGGATGG CAGAGATGTT GGAGGTGCTC 
ATCACCTCTG CCGATGACGA CCACTTCAGC GGCATAATTC CCTCCATGAA CAGGGAGCGA 
CTGTTCCGGA ACCACCCATT TGTAGCGGAG CTGCCTTCCA CGCTGCTTCA CGTCTTTGTG 
AGCATCGAGA TGACGGGCCA GAGCGTCACC TTTGAGCAGA AGTTCCACTA CCGCCGTCCC 
ATGTACATTG TGTTGGACCA CCTGTGGAAC ATGCCTGACC ATCGGAACAA GATGAAGAGT 
CTGGCAGCTG AAGCAGAGGC CAACATAGAA TGTTCCAGTC CTCCACTTTT CCTGAGATTC 
ATTAACCTTC TCATCAATGA TGCCATTTTT CTCTTGGACG AAGCCCTTTC GTACATGAGC 
CGCCTGCGCG AGCTGCAGCA GGAACGCCAG CAGCAGCAGC CTCTGGGGGG CCCTTTGTGG 
AGCCAGGGGG AGGCAAACAT GCAGCACGTG GGCATGCTGG CCCACTTCCA CAACGTCATG 
GGCACCGAGA CCATCCGCAC CCTGGCCTGG CTCACCACCG AGATCAAGTC GCTCTTTTGC 
CACCCGACCA TGGTGGACCG CATCGCAACC ATGCTGAACT ACTTCCTACT CCACCTGGTT 
GGACCCCAGA AGAAGAACCT GAAGGTGAAG GACTTCAGCG AGTACGAGTT TAAGCCTCAG 
GAGCTGGTGC AGAACATCTG CAGGATCTAC ACCAACCTGG GCTGTGCCAA CTCCGAGACC 
GCGCAGGCAT TCTGCGTGGC CGTGTCCAGG GACGGCAGGT CATACAGCCC TGAACTGTTC 
CCCCAAGCAC AGAGCGTCCT CTTGAAAATA GGCCAGAGCA TTCTATCCAT TGGAGTCGGT 
GAACTAGCAG AAAAAATTCT GGTATGTATT GGTGGACCCG AGCGACCAGA ACCTCTCTTA 
CTACCCAGCT GTGAGAGATG GGCAGAAGGT CCATTACAAG AAGATCTTCA GCACCTTTCC 
GACAAGTGTG TCTATGACTC TTTTGTTCTC GAAAGCTCCG GCTCATCCTT TGTCGGCTTC 
CTTTCCAGTG ACCAGACTGA CCCTTTCAAC CGGTCTCCCC TGACAATGGA GATGGTGACC 
TCGGACAACG ACCTCAAGAA CAAGATCCTG GAGTGGAGAG CAAAACGAAT GACCAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002412693.1
CDS	1..2820
Translation	MQLVFERLVLDDPQNHVVSKSKYADQQALADAGETRVVFYLYQCFSRLNKVAAKLEVPDPEHQSASLTIVSQASTCLCHCEIYPGKKIPQQVFYLLQEYHAEGGHQDSLESFLKKCADCIKGRFDAEEEQVSLLEVLSHSFNLLKKKFGKVSIANRELFVLIDILKYFTKSVSMAMALLDFSTPAELVDLRSFQESLLGAPLCVSCLPRKEADPFEFFEKPSQALMNEHAITESNLWLPLKGLAVELHNLFYSLLRLSPETRNRTLTWIGHCITACSERGKLWNNQVSELFMAVQSGDGFALNLGAVLLRLARPFSEPYSPKLLKVQHQYCSFEPKSEQEANASCMHIRGLSKETCLVPREEGAASESPCGPFNFSTECFFACQRILSLGFRVVHERLARLSQDLNRVRRVYEEARSQGGDSSEVTRRLQDNMEKGMTRFLSLKAVLLEPDSLEHMLQFHVACATWLCHLATAERLDAFQPLCLPFPEHGNARLAYVPEFVIENICDCIVFVKRFSEKSLALVGPNLEHLMTLILLFMGSPQRMNNPHLRARMAEMLEVLITSADDDHFSGIIPSMNRERLFRNHPFVAELPSTLLHVFVSIEMTGQSVTFEQKFHYRRPMYIVLDHLWNMPDHRNKMKSLAAEAEANIECSSPPLFLRFINLLINDAIFLLDEALSYMSRLRELQQERQQQQPLGGPLWSQGEANMQHVGMLAHFHNVMGTETIRTLAWLTTEIKSLFCHPTMVDRIATMLNYFLLHLVGPQKKNLKVKDFSEYEFKPQELVQNICRIYTNLGCANSETAQAFCVAVSRDGRSYSPELFPQAQSVLLKIGQSILSIGVGELAEKILVCIGGPERPEPLLLPSCERWAEGPLQEDLQHLSDKCVYDSFVLESSGSSFVGFLSSDQTDPFNRSPLTMEMVTSDNDLKNKILEWRAKRMTN

Target ORF information:

RefSeq Version	XM_002412648.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis ubiquitination factor E4a, putative, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002412648.1

ORF Insert Sequence:

ATGCAGCTGG TCTTCGAGCG GCTTGTGTTG GACGACCCCC AGAACCACGT GGTCAGCAAG 
AGCAAGTATG CCGACCAGCA GGCTCTGGCT GATGCCGGAG AAACCAGAGT GGTCTTCTAC 
CTCTACCAGT GCTTCTCGAG GCTCAACAAG GTGGCCGCCA AGCTGGAGGT ACCAGATCCC 
GAGCATCAGT CCGCTTCCCT CACGATTGTG TCGCAGGCGT CGACTTGCCT TTGCCACTGT 
GAAATCTACC CTGGCAAAAA GATACCGCAG CAGGTCTTCT ACCTACTCCA GGAGTATCAC 
GCTGAAGGAG GTCACCAAGA CAGCCTGGAG TCTTTTCTGA AGAAATGCGC AGACTGTATC 
AAGGGCCGCT TTGATGCAGA GGAAGAGCAG GTTTCTCTGC TTGAAGTGCT CTCGCATTCC 
TTCAACTTGC TCAAGAAGAA ATTCGGCAAG GTGTCCATCG CTAACCGGGA ACTGTTTGTG 
CTTATCGACA TCCTCAAGTA CTTCACCAAA TCTGTTTCCA TGGCAATGGC ACTCTTAGAC 
TTCAGCACGC CTGCAGAGCT GGTTGACCTA CGCAGTTTCC AGGAGAGCCT GCTCGGTGCC 
CCCCTGTGCG TGTCCTGCCT GCCACGCAAG GAAGCCGACC CCTTCGAGTT CTTCGAGAAG 
CCCTCTCAGG CGCTGATGAA CGAGCATGCC ATCACCGAGA GCAATCTGTG GCTGCCGCTA 
AAAGGTCTCG CAGTGGAGCT GCACAACCTG TTTTATTCCC TGCTTCGGCT TTCACCAGAG 
ACACGCAACA GGACCCTGAC ATGGATAGGA CACTGCATCA CTGCATGTTC AGAGCGGGGC 
AAACTGTGGA ACAACCAGGT GTCTGAGCTG TTCATGGCGG TGCAATCCGG CGACGGCTTT 
GCCCTGAACC TGGGGGCGGT GCTGCTGCGG CTGGCGCGGC CCTTCTCGGA GCCCTACTCT 
CCCAAGCTGC TCAAGGTACA GCACCAGTAC TGCTCCTTCG AGCCAAAGTC GGAGCAGGAA 
GCCAATGCCA GTTGCATGCA CATCAGAGGG CTGTCCAAGG AGACGTGCCT GGTGCCGAGG 
GAGGAGGGTG CGGCCAGCGA GTCCCCTTGC GGCCCCTTCA ACTTCTCGAC GGAGTGCTTC 
TTTGCCTGCC AACGCATCCT GAGCCTGGGC TTCCGGGTGG TGCACGAACG GCTGGCCCGG 
CTCAGCCAGG ACCTGAACCG GGTGCGCAGG GTCTACGAGG AGGCACGCTC GCAGGGCGGC 
GACAGCTCTG AGGTCACCCG CAGGCTGCAG GACAATATGG AGAAGGGCAT GACCCGGTTC 
CTGTCCCTGA AGGCGGTGCT CTTGGAGCCG GACTCCCTGG AGCACATGCT GCAGTTCCAC 
GTGGCCTGCG CCACCTGGCT GTGCCACCTG GCCACGGCCG AGAGGCTGGA CGCCTTCCAG 
CCCCTGTGCC TGCCTTTCCC CGAGCACGGC AACGCCAGGC TCGCCTATGT TCCGGAGTTT 
GTCATTGAGA ACATCTGTGA CTGCATTGTG TTTGTCAAGC GCTTCAGCGA AAAAAGTCTG 
GCGCTCGTGG GCCCGAACTT GGAGCACCTG ATGACGCTGA TACTGCTGTT CATGGGCAGC 
CCCCAGAGAA TGAACAACCC GCACCTAAGA GCCAGGATGG CAGAGATGTT GGAGGTGCTC 
ATCACCTCTG CCGATGACGA CCACTTCAGC GGCATAATTC CCTCCATGAA CAGGGAGCGA 
CTGTTCCGGA ACCACCCATT TGTAGCGGAG CTGCCTTCCA CGCTGCTTCA CGTCTTTGTG 
AGCATCGAGA TGACGGGCCA GAGCGTCACC TTTGAGCAGA AGTTCCACTA CCGCCGTCCC 
ATGTACATTG TGTTGGACCA CCTGTGGAAC ATGCCTGACC ATCGGAACAA GATGAAGAGT 
CTGGCAGCTG AAGCAGAGGC CAACATAGAA TGTTCCAGTC CTCCACTTTT CCTGAGATTC 
ATTAACCTTC TCATCAATGA TGCCATTTTT CTCTTGGACG AAGCCCTTTC GTACATGAGC 
CGCCTGCGCG AGCTGCAGCA GGAACGCCAG CAGCAGCAGC CTCTGGGGGG CCCTTTGTGG 
AGCCAGGGGG AGGCAAACAT GCAGCACGTG GGCATGCTGG CCCACTTCCA CAACGTCATG 
GGCACCGAGA CCATCCGCAC CCTGGCCTGG CTCACCACCG AGATCAAGTC GCTCTTTTGC 
CACCCGACCA TGGTGGACCG CATCGCAACC ATGCTGAACT ACTTCCTACT CCACCTGGTT 
GGACCCCAGA AGAAGAACCT GAAGGTGAAG GACTTCAGCG AGTACGAGTT TAAGCCTCAG 
GAGCTGGTGC AGAACATCTG CAGGATCTAC ACCAACCTGG GCTGTGCCAA CTCCGAGACC 
GCGCAGGCAT TCTGCGTGGC CGTGTCCAGG GACGGCAGGT CATACAGCCC TGAACTGTTC 
CCCCAAGCAC AGAGCGTCCT CTTGAAAATA GGCCAGAGCA TTCTATCCAT TGGAGTCGGT 
GAACTAGCAG AAAAAATTCT GGTATGTATT GGTGGACCCG AGCGACCAGA ACCTCTCTTA 
CTACCCAGCT GTGAGAGATG GGCAGAAGGT CCATTACAAG AAGATCTTCA GCACCTTTCC 
GACAAGTGTG TCTATGACTC TTTTGTTCTC GAAAGCTCCG GCTCATCCTT TGTCGGCTTC 
CTTTCCAGTG ACCAGACTGA CCCTTTCAAC CGGTCTCCCC TGACAATGGA GATGGTGACC 
TCGGACAACG ACCTCAAGAA CAAGATCCTG GAGTGGAGAG CAAAACGAAT GACCAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8038480 ( XM_029978626.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029834486.1 Ixodes scapularis ubiquitin conjugation factor E4 A (LOC8038480), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OIa21841
Clone ID Related Accession (Same CDS sequence)	XM_029978626.1 , XM_029978627.1
Accession Version	XM_029978626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	ubiquitin conjugation factor E4 A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919160.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACT CGTCGGACAT GGCGCGCAAC CCCTTCGCCG CTCTGTTTCC GTCCATGGTG 
GACGTGCAAG CCTTCGTCCA GAGCGGGGCG GAGACGGCCC CGCGAGACTT CCCGCCGCTG 
CCGGTGACGG AGAAGGCCCT GGCCACGCAG GACATAGACG CCAATGGGGT CCCGGTCCTC 
ATCGGTCACG TAGACGACGC CACGTCCGAC GACCTGGCGT CGCTGCTCGA AGAGATCTTC 
AGGGTCACCA CCTCTGACTG TAATGATGCC AAGACATCCA AGGGTCCCCC GCACTGTGTG 
CTACTGCCAA CTTTCAAGGA TTCCAAGAAA TGTCTCGACC TGAACACCCT TAATGAGCTG 
GTCTTCGAGC GGCTTGTGTT GGACGACCCC CAGAACCACG TGGTCAGCAA GAGCAAGTAT 
GCCGACCAGC AGGCTCTGGC TGATGCCGGA GAAACCAGGG TGGTCTTCTA CCTCTACCAG 
TGCTTCTCGA GGCTCAACAA GGTGGCCGCC AAGCTGGAGA ATGAAGATTA CGAAACTATC 
GAGAAGACGA TTGTGTCGCA GGCGTCGACT TGCCTTTGCC ACTGTGAAAT CTACCCTGGC 
AAAAAGATAC CGCAGCAGGT CTTCTACCTA CTCCAGGAGT ATCACGCTGA AGGAGGTCAC 
CAAGACAGCC TGGAGTCTTT TCTGAAGAAA TGCGCAGACT GTATCAAGGG CCGCTTTGAT 
GCAGAGGAAG AGCAGGTTTC TCTGCTTGAA GTGCTCTCGC ATTCCTTCAA CTTGCTCAAG 
AAGAAATTCG GCAAGGTGTC TATCGCCAAC CGGGAACTGT TTGTGCTTAT CGACATCCTC 
AAGTACTTCA CCAAATCTGT TTCCATGGCA ATGGCACTCT TAGACTTCAG CACGCCTGCA 
GAGCTGGTTG ACCTACGCAG TTTCCAGGAG AGCCTGCTCG GTGCCCCCCT GTGCGTGTCC 
TGCCTGCCAC GCAAGGAAGC CGACCCCTTC GAGTTCTTCG AGAAGCCCTC TCAGGCGCTG 
ATGAACGAGC ATGCCATCAC CGAGAGCAAT CTGTGGCTGC CGCTAAAAGG TCTCGCAGTG 
GAGCTGCACA ACTTGTTTTA TTCCCTGCTT CGGCTTTCAC CAGAGACACG CAACAGGACC 
CTGACATGGA TAGGACACTG CATCACTGCA TGTTCAGAGC GGGGCAAACT GTGGAACAAC 
CAGGTGTCTG AGCTGTTCAT GGCAGTGCAA TCCGGCGACG GCTTTGCCCT GAACCTGGGG 
GCAGTGCTGC TGCGGCTGGC GCGGCCCTTC TCGGAGCCCT ACTCTCCCAA GCTGCTCAAG 
GTGCAGCACC AGTACTGCTC CTTCGAGCCA AAGTCGGAGC AGGAAGCCAA TGCCAGTTGC 
ATGCACATCA GAGGGCTGTC CAAGGAGACG TGCCTGGTGC CGAGGGAGGA GGGTGCGGCC 
AGCGAGTCCC CTTGCGGCCC CTTCAACTTC TCGACGGAGT GCTTCTTTGC CTGCCAACGC 
ATCCTGAGCC TGGGCTTCCG GGTGGTGCAC GAACGGCTGG CCCGGCTCAG CCAGGACCTG 
AACCGGGTGC GCAGGGTCTA CGAGGAGGCA CGCTCGCAGG GCGGCGACAG CTCTGAGGTC 
ACCCGCAGGC TGCAGGACAA TATGGAGAAG GGCATGACCC GGTTCCTGTC CCTGAAGGCG 
GTGCTCTTGG AGCCGGACTC CCTGGAGCAC ATGCTGCAGT TCCACGTGGC CTGCGCCACC 
TGGCTGTGCC ACCTGGCCAC GGCTGAGAGG CTGGACGCCT TCCAGCCCCT GTGCCTGCCT 
TTCCCCGAGC ACGGCAACGC CAGGCTCGCC TATGTTCCGG AGTTTGTCAT TGAGAACATC 
TGTGACTGCA TTGTGTTTGT CAAGCGCTTC AGCGAAAAAA GTCTGGCGCT CGTGGGCCCG 
AACTTGGAGC ACCTGATGAC GCTGATACTG CTGTTCATGG GCAGCCCCCA GAGAATGAAC 
AACCCGCACC TAAGAGCCAG GATGGCAGAG ATGTTGGAGG TGCTCATCAC CTCTGCCGAT 
GACGACCACT TCAGCGGCAT AATTCCCTCC ATGAACAGGG AGCGACTGTT CCGGAACCAC 
CCATTTGTAG CGGAGCTGCC TTCCACGCTG CTTCACGTCT TTGTGAGCAT CGAGATGACG 
GGCCAGAGCG TCACCTTTGA GCAGAAGTTC CACTACCGCC GTCCCATGTA CATTGTGTTG 
GACCACCTGT GGAACATGCC TGACCATCGG AACAAGATGA AGAGTCTGGC AGCTGAAGCA 
GAGGCCAACA TAGAATGTTC CAGTCCTCCA CTTTTCCTGA GATTCATTAA CCTTCTCATC 
AATGATGCCA TTTTTCTCTT GGACGAAGCC CTTTCGTACA TGAGCCGCCT GCGCGAGCTG 
CAGCAGGAAC GCCAGCAGCA GCAGCCTCTG GGGGGCCCTT TGTGGAGCCA GGGGGAGGCA 
AACATGCAGC ACGTGGGCAT GCTGGCCCAC TTCCACAACG TCATGGGCAC CGAGACCATC 
CGCACCCTGG CCTGGCTCAC CACCGAGATC AAGTCGCTCT TTTGCCACCC GACCATGGTG 
GACCGCATCG CAACCATGCT GAACTACTTC CTACTCCACC TGGTTGGACC CCAGAAGAAG 
AACCTGAAGG TGAAGGACTT CAGCGAGTAC GAGTTTAAGC CTCAGGAGCT GGTGCAGAAC 
ATCTGCAGGA TCTACACCAA CCTGGGCTGT GCCAACTCCG AGACCGCGCA GGCATTCTGC 
GTGGCCGTGT CCAGGGACGG CAGGTCATAC AGCCCTGAAC TGTTCCCCCA AGCACAGAGC 
GTCCTCTTGA AAATAGGCCA GAGCATTCTA TCCATTGGAG TCGGTGAACT AGCAGAAAAA 
ATTCTGAAAG CGGCGTCACA TCAGAAGCAA GAGGATGAAG CTATTGGAGA TGCGCCTGAA 
GAGTTCCTGG ACCCAATCAT GAGTACCATC ATGAACGACC CGGTGGTCCT GCCGAGTTCT 
GGCATCACCC TGGACCGTTC CACCATAGCA AGACATCTGC TCAGTGACCA GACTGACCCT 
TTCAACCGGT CTCCCCTGAC AATGGAGATG GTGACCTCGG ACAACGACCT CAAGAACAAG 
ATCCTGGAGT GGAGAGCAAA ACGAATGACC AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029834486.1
CDS	255..3410
Translation	MSDSSDMARNPFAALFPSMVDVQAFVQSGAETAPRDFPPLPVTEKALATQDIDANGVPVLIGHVDDATSDDLASLLEEIFRVTTSDCNDAKTSKGPPHCVLLPTFKDSKKCLDLNTLNELVFERLVLDDPQNHVVSKSKYADQQALADAGETRVVFYLYQCFSRLNKVAAKLENEDYETIEKTIVSQASTCLCHCEIYPGKKIPQQVFYLLQEYHAEGGHQDSLESFLKKCADCIKGRFDAEEEQVSLLEVLSHSFNLLKKKFGKVSIANRELFVLIDILKYFTKSVSMAMALLDFSTPAELVDLRSFQESLLGAPLCVSCLPRKEADPFEFFEKPSQALMNEHAITESNLWLPLKGLAVELHNLFYSLLRLSPETRNRTLTWIGHCITACSERGKLWNNQVSELFMAVQSGDGFALNLGAVLLRLARPFSEPYSPKLLKVQHQYCSFEPKSEQEANASCMHIRGLSKETCLVPREEGAASESPCGPFNFSTECFFACQRILSLGFRVVHERLARLSQDLNRVRRVYEEARSQGGDSSEVTRRLQDNMEKGMTRFLSLKAVLLEPDSLEHMLQFHVACATWLCHLATAERLDAFQPLCLPFPEHGNARLAYVPEFVIENICDCIVFVKRFSEKSLALVGPNLEHLMTLILLFMGSPQRMNNPHLRARMAEMLEVLITSADDDHFSGIIPSMNRERLFRNHPFVAELPSTLLHVFVSIEMTGQSVTFEQKFHYRRPMYIVLDHLWNMPDHRNKMKSLAAEAEANIECSSPPLFLRFINLLINDAIFLLDEALSYMSRLRELQQERQQQQPLGGPLWSQGEANMQHVGMLAHFHNVMGTETIRTLAWLTTEIKSLFCHPTMVDRIATMLNYFLLHLVGPQKKNLKVKDFSEYEFKPQELVQNICRIYTNLGCANSETAQAFCVAVSRDGRSYSPELFPQAQSVLLKIGQSILSIGVGELAEKILKAASHQKQEDEAIGDAPEEFLDPIMSTIMNDPVVLPSSGITLDRSTIARHLLSDQTDPFNRSPLTMEMVTSDNDLKNKILEWRAKRMTN

Target ORF information:

RefSeq Version	XM_029978626.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis ubiquitin conjugation factor E4 A (LOC8038480), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029978626.1

ORF Insert Sequence:

ATGTCGGACT CGTCGGACAT GGCGCGCAAC CCCTTCGCCG CTCTGTTTCC GTCCATGGTG 
GACGTGCAAG CCTTCGTCCA GAGCGGGGCG GAGACGGCCC CGCGAGACTT CCCGCCGCTG 
CCGGTGACGG AGAAGGCCCT GGCCACGCAG GACATAGACG CCAATGGGGT CCCGGTCCTC 
ATCGGTCACG TAGACGACGC CACGTCCGAC GACCTGGCGT CGCTGCTCGA AGAGATCTTC 
AGGGTCACCA CCTCTGACTG TAATGATGCC AAGACATCCA AGGGTCCCCC GCACTGTGTG 
CTACTGCCAA CTTTCAAGGA TTCCAAGAAA TGTCTCGACC TGAACACCCT TAATGAGCTG 
GTCTTCGAGC GGCTTGTGTT GGACGACCCC CAGAACCACG TGGTCAGCAA GAGCAAGTAT 
GCCGACCAGC AGGCTCTGGC TGATGCCGGA GAAACCAGGG TGGTCTTCTA CCTCTACCAG 
TGCTTCTCGA GGCTCAACAA GGTGGCCGCC AAGCTGGAGA ATGAAGATTA CGAAACTATC 
GAGAAGACGA TTGTGTCGCA GGCGTCGACT TGCCTTTGCC ACTGTGAAAT CTACCCTGGC 
AAAAAGATAC CGCAGCAGGT CTTCTACCTA CTCCAGGAGT ATCACGCTGA AGGAGGTCAC 
CAAGACAGCC TGGAGTCTTT TCTGAAGAAA TGCGCAGACT GTATCAAGGG CCGCTTTGAT 
GCAGAGGAAG AGCAGGTTTC TCTGCTTGAA GTGCTCTCGC ATTCCTTCAA CTTGCTCAAG 
AAGAAATTCG GCAAGGTGTC TATCGCCAAC CGGGAACTGT TTGTGCTTAT CGACATCCTC 
AAGTACTTCA CCAAATCTGT TTCCATGGCA ATGGCACTCT TAGACTTCAG CACGCCTGCA 
GAGCTGGTTG ACCTACGCAG TTTCCAGGAG AGCCTGCTCG GTGCCCCCCT GTGCGTGTCC 
TGCCTGCCAC GCAAGGAAGC CGACCCCTTC GAGTTCTTCG AGAAGCCCTC TCAGGCGCTG 
ATGAACGAGC ATGCCATCAC CGAGAGCAAT CTGTGGCTGC CGCTAAAAGG TCTCGCAGTG 
GAGCTGCACA ACTTGTTTTA TTCCCTGCTT CGGCTTTCAC CAGAGACACG CAACAGGACC 
CTGACATGGA TAGGACACTG CATCACTGCA TGTTCAGAGC GGGGCAAACT GTGGAACAAC 
CAGGTGTCTG AGCTGTTCAT GGCAGTGCAA TCCGGCGACG GCTTTGCCCT GAACCTGGGG 
GCAGTGCTGC TGCGGCTGGC GCGGCCCTTC TCGGAGCCCT ACTCTCCCAA GCTGCTCAAG 
GTGCAGCACC AGTACTGCTC CTTCGAGCCA AAGTCGGAGC AGGAAGCCAA TGCCAGTTGC 
ATGCACATCA GAGGGCTGTC CAAGGAGACG TGCCTGGTGC CGAGGGAGGA GGGTGCGGCC 
AGCGAGTCCC CTTGCGGCCC CTTCAACTTC TCGACGGAGT GCTTCTTTGC CTGCCAACGC 
ATCCTGAGCC TGGGCTTCCG GGTGGTGCAC GAACGGCTGG CCCGGCTCAG CCAGGACCTG 
AACCGGGTGC GCAGGGTCTA CGAGGAGGCA CGCTCGCAGG GCGGCGACAG CTCTGAGGTC 
ACCCGCAGGC TGCAGGACAA TATGGAGAAG GGCATGACCC GGTTCCTGTC CCTGAAGGCG 
GTGCTCTTGG AGCCGGACTC CCTGGAGCAC ATGCTGCAGT TCCACGTGGC CTGCGCCACC 
TGGCTGTGCC ACCTGGCCAC GGCTGAGAGG CTGGACGCCT TCCAGCCCCT GTGCCTGCCT 
TTCCCCGAGC ACGGCAACGC CAGGCTCGCC TATGTTCCGG AGTTTGTCAT TGAGAACATC 
TGTGACTGCA TTGTGTTTGT CAAGCGCTTC AGCGAAAAAA GTCTGGCGCT CGTGGGCCCG 
AACTTGGAGC ACCTGATGAC GCTGATACTG CTGTTCATGG GCAGCCCCCA GAGAATGAAC 
AACCCGCACC TAAGAGCCAG GATGGCAGAG ATGTTGGAGG TGCTCATCAC CTCTGCCGAT 
GACGACCACT TCAGCGGCAT AATTCCCTCC ATGAACAGGG AGCGACTGTT CCGGAACCAC 
CCATTTGTAG CGGAGCTGCC TTCCACGCTG CTTCACGTCT TTGTGAGCAT CGAGATGACG 
GGCCAGAGCG TCACCTTTGA GCAGAAGTTC CACTACCGCC GTCCCATGTA CATTGTGTTG 
GACCACCTGT GGAACATGCC TGACCATCGG AACAAGATGA AGAGTCTGGC AGCTGAAGCA 
GAGGCCAACA TAGAATGTTC CAGTCCTCCA CTTTTCCTGA GATTCATTAA CCTTCTCATC 
AATGATGCCA TTTTTCTCTT GGACGAAGCC CTTTCGTACA TGAGCCGCCT GCGCGAGCTG 
CAGCAGGAAC GCCAGCAGCA GCAGCCTCTG GGGGGCCCTT TGTGGAGCCA GGGGGAGGCA 
AACATGCAGC ACGTGGGCAT GCTGGCCCAC TTCCACAACG TCATGGGCAC CGAGACCATC 
CGCACCCTGG CCTGGCTCAC CACCGAGATC AAGTCGCTCT TTTGCCACCC GACCATGGTG 
GACCGCATCG CAACCATGCT GAACTACTTC CTACTCCACC TGGTTGGACC CCAGAAGAAG 
AACCTGAAGG TGAAGGACTT CAGCGAGTAC GAGTTTAAGC CTCAGGAGCT GGTGCAGAAC 
ATCTGCAGGA TCTACACCAA CCTGGGCTGT GCCAACTCCG AGACCGCGCA GGCATTCTGC 
GTGGCCGTGT CCAGGGACGG CAGGTCATAC AGCCCTGAAC TGTTCCCCCA AGCACAGAGC 
GTCCTCTTGA AAATAGGCCA GAGCATTCTA TCCATTGGAG TCGGTGAACT AGCAGAAAAA 
ATTCTGAAAG CGGCGTCACA TCAGAAGCAA GAGGATGAAG CTATTGGAGA TGCGCCTGAA 
GAGTTCCTGG ACCCAATCAT GAGTACCATC ATGAACGACC CGGTGGTCCT GCCGAGTTCT 
GGCATCACCC TGGACCGTTC CACCATAGCA AGACATCTGC TCAGTGACCA GACTGACCCT 
TTCAACCGGT CTCCCCTGAC AATGGAGATG GTGACCTCGG ACAACGACCT CAAGAACAAG 
ATCCTGGAGT GGAGAGCAAA ACGAATGACC AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8038480 ( XM_029978627.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029834487.1 Ixodes scapularis ubiquitin conjugation factor E4 A (LOC8038480), transcript variant X2, mRNA.

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CloneID	OIa21841
Clone ID Related Accession (Same CDS sequence)	XM_029978626.1 , XM_029978627.1
Accession Version	XM_029978627.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	ubiquitin conjugation factor E4 A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021919160.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGACT CGTCGGACAT GGCGCGCAAC CCCTTCGCCG CTCTGTTTCC GTCCATGGTG 
GACGTGCAAG CCTTCGTCCA GAGCGGGGCG GAGACGGCCC CGCGAGACTT CCCGCCGCTG 
CCGGTGACGG AGAAGGCCCT GGCCACGCAG GACATAGACG CCAATGGGGT CCCGGTCCTC 
ATCGGTCACG TAGACGACGC CACGTCCGAC GACCTGGCGT CGCTGCTCGA AGAGATCTTC 
AGGGTCACCA CCTCTGACTG TAATGATGCC AAGACATCCA AGGGTCCCCC GCACTGTGTG 
CTACTGCCAA CTTTCAAGGA TTCCAAGAAA TGTCTCGACC TGAACACCCT TAATGAGCTG 
GTCTTCGAGC GGCTTGTGTT GGACGACCCC CAGAACCACG TGGTCAGCAA GAGCAAGTAT 
GCCGACCAGC AGGCTCTGGC TGATGCCGGA GAAACCAGGG TGGTCTTCTA CCTCTACCAG 
TGCTTCTCGA GGCTCAACAA GGTGGCCGCC AAGCTGGAGA ATGAAGATTA CGAAACTATC 
GAGAAGACGA TTGTGTCGCA GGCGTCGACT TGCCTTTGCC ACTGTGAAAT CTACCCTGGC 
AAAAAGATAC CGCAGCAGGT CTTCTACCTA CTCCAGGAGT ATCACGCTGA AGGAGGTCAC 
CAAGACAGCC TGGAGTCTTT TCTGAAGAAA TGCGCAGACT GTATCAAGGG CCGCTTTGAT 
GCAGAGGAAG AGCAGGTTTC TCTGCTTGAA GTGCTCTCGC ATTCCTTCAA CTTGCTCAAG 
AAGAAATTCG GCAAGGTGTC TATCGCCAAC CGGGAACTGT TTGTGCTTAT CGACATCCTC 
AAGTACTTCA CCAAATCTGT TTCCATGGCA ATGGCACTCT TAGACTTCAG CACGCCTGCA 
GAGCTGGTTG ACCTACGCAG TTTCCAGGAG AGCCTGCTCG GTGCCCCCCT GTGCGTGTCC 
TGCCTGCCAC GCAAGGAAGC CGACCCCTTC GAGTTCTTCG AGAAGCCCTC TCAGGCGCTG 
ATGAACGAGC ATGCCATCAC CGAGAGCAAT CTGTGGCTGC CGCTAAAAGG TCTCGCAGTG 
GAGCTGCACA ACTTGTTTTA TTCCCTGCTT CGGCTTTCAC CAGAGACACG CAACAGGACC 
CTGACATGGA TAGGACACTG CATCACTGCA TGTTCAGAGC GGGGCAAACT GTGGAACAAC 
CAGGTGTCTG AGCTGTTCAT GGCAGTGCAA TCCGGCGACG GCTTTGCCCT GAACCTGGGG 
GCAGTGCTGC TGCGGCTGGC GCGGCCCTTC TCGGAGCCCT ACTCTCCCAA GCTGCTCAAG 
GTGCAGCACC AGTACTGCTC CTTCGAGCCA AAGTCGGAGC AGGAAGCCAA TGCCAGTTGC 
ATGCACATCA GAGGGCTGTC CAAGGAGACG TGCCTGGTGC CGAGGGAGGA GGGTGCGGCC 
AGCGAGTCCC CTTGCGGCCC CTTCAACTTC TCGACGGAGT GCTTCTTTGC CTGCCAACGC 
ATCCTGAGCC TGGGCTTCCG GGTGGTGCAC GAACGGCTGG CCCGGCTCAG CCAGGACCTG 
AACCGGGTGC GCAGGGTCTA CGAGGAGGCA CGCTCGCAGG GCGGCGACAG CTCTGAGGTC 
ACCCGCAGGC TGCAGGACAA TATGGAGAAG GGCATGACCC GGTTCCTGTC CCTGAAGGCG 
GTGCTCTTGG AGCCGGACTC CCTGGAGCAC ATGCTGCAGT TCCACGTGGC CTGCGCCACC 
TGGCTGTGCC ACCTGGCCAC GGCTGAGAGG CTGGACGCCT TCCAGCCCCT GTGCCTGCCT 
TTCCCCGAGC ACGGCAACGC CAGGCTCGCC TATGTTCCGG AGTTTGTCAT TGAGAACATC 
TGTGACTGCA TTGTGTTTGT CAAGCGCTTC AGCGAAAAAA GTCTGGCGCT CGTGGGCCCG 
AACTTGGAGC ACCTGATGAC GCTGATACTG CTGTTCATGG GCAGCCCCCA GAGAATGAAC 
AACCCGCACC TAAGAGCCAG GATGGCAGAG ATGTTGGAGG TGCTCATCAC CTCTGCCGAT 
GACGACCACT TCAGCGGCAT AATTCCCTCC ATGAACAGGG AGCGACTGTT CCGGAACCAC 
CCATTTGTAG CGGAGCTGCC TTCCACGCTG CTTCACGTCT TTGTGAGCAT CGAGATGACG 
GGCCAGAGCG TCACCTTTGA GCAGAAGTTC CACTACCGCC GTCCCATGTA CATTGTGTTG 
GACCACCTGT GGAACATGCC TGACCATCGG AACAAGATGA AGAGTCTGGC AGCTGAAGCA 
GAGGCCAACA TAGAATGTTC CAGTCCTCCA CTTTTCCTGA GATTCATTAA CCTTCTCATC 
AATGATGCCA TTTTTCTCTT GGACGAAGCC CTTTCGTACA TGAGCCGCCT GCGCGAGCTG 
CAGCAGGAAC GCCAGCAGCA GCAGCCTCTG GGGGGCCCTT TGTGGAGCCA GGGGGAGGCA 
AACATGCAGC ACGTGGGCAT GCTGGCCCAC TTCCACAACG TCATGGGCAC CGAGACCATC 
CGCACCCTGG CCTGGCTCAC CACCGAGATC AAGTCGCTCT TTTGCCACCC GACCATGGTG 
GACCGCATCG CAACCATGCT GAACTACTTC CTACTCCACC TGGTTGGACC CCAGAAGAAG 
AACCTGAAGG TGAAGGACTT CAGCGAGTAC GAGTTTAAGC CTCAGGAGCT GGTGCAGAAC 
ATCTGCAGGA TCTACACCAA CCTGGGCTGT GCCAACTCCG AGACCGCGCA GGCATTCTGC 
GTGGCCGTGT CCAGGGACGG CAGGTCATAC AGCCCTGAAC TGTTCCCCCA AGCACAGAGC 
GTCCTCTTGA AAATAGGCCA GAGCATTCTA TCCATTGGAG TCGGTGAACT AGCAGAAAAA 
ATTCTGAAAG CGGCGTCACA TCAGAAGCAA GAGGATGAAG CTATTGGAGA TGCGCCTGAA 
GAGTTCCTGG ACCCAATCAT GAGTACCATC ATGAACGACC CGGTGGTCCT GCCGAGTTCT 
GGCATCACCC TGGACCGTTC CACCATAGCA AGACATCTGC TCAGTGACCA GACTGACCCT 
TTCAACCGGT CTCCCCTGAC AATGGAGATG GTGACCTCGG ACAACGACCT CAAGAACAAG 
ATCCTGGAGT GGAGAGCAAA ACGAATGACC AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029834487.1
CDS	240..3395
Translation	MSDSSDMARNPFAALFPSMVDVQAFVQSGAETAPRDFPPLPVTEKALATQDIDANGVPVLIGHVDDATSDDLASLLEEIFRVTTSDCNDAKTSKGPPHCVLLPTFKDSKKCLDLNTLNELVFERLVLDDPQNHVVSKSKYADQQALADAGETRVVFYLYQCFSRLNKVAAKLENEDYETIEKTIVSQASTCLCHCEIYPGKKIPQQVFYLLQEYHAEGGHQDSLESFLKKCADCIKGRFDAEEEQVSLLEVLSHSFNLLKKKFGKVSIANRELFVLIDILKYFTKSVSMAMALLDFSTPAELVDLRSFQESLLGAPLCVSCLPRKEADPFEFFEKPSQALMNEHAITESNLWLPLKGLAVELHNLFYSLLRLSPETRNRTLTWIGHCITACSERGKLWNNQVSELFMAVQSGDGFALNLGAVLLRLARPFSEPYSPKLLKVQHQYCSFEPKSEQEANASCMHIRGLSKETCLVPREEGAASESPCGPFNFSTECFFACQRILSLGFRVVHERLARLSQDLNRVRRVYEEARSQGGDSSEVTRRLQDNMEKGMTRFLSLKAVLLEPDSLEHMLQFHVACATWLCHLATAERLDAFQPLCLPFPEHGNARLAYVPEFVIENICDCIVFVKRFSEKSLALVGPNLEHLMTLILLFMGSPQRMNNPHLRARMAEMLEVLITSADDDHFSGIIPSMNRERLFRNHPFVAELPSTLLHVFVSIEMTGQSVTFEQKFHYRRPMYIVLDHLWNMPDHRNKMKSLAAEAEANIECSSPPLFLRFINLLINDAIFLLDEALSYMSRLRELQQERQQQQPLGGPLWSQGEANMQHVGMLAHFHNVMGTETIRTLAWLTTEIKSLFCHPTMVDRIATMLNYFLLHLVGPQKKNLKVKDFSEYEFKPQELVQNICRIYTNLGCANSETAQAFCVAVSRDGRSYSPELFPQAQSVLLKIGQSILSIGVGELAEKILKAASHQKQEDEAIGDAPEEFLDPIMSTIMNDPVVLPSSGITLDRSTIARHLLSDQTDPFNRSPLTMEMVTSDNDLKNKILEWRAKRMTN

Target ORF information:

RefSeq Version	XM_029978627.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis ubiquitin conjugation factor E4 A (LOC8038480), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029978627.1

ORF Insert Sequence:

ATGTCGGACT CGTCGGACAT GGCGCGCAAC CCCTTCGCCG CTCTGTTTCC GTCCATGGTG 
GACGTGCAAG CCTTCGTCCA GAGCGGGGCG GAGACGGCCC CGCGAGACTT CCCGCCGCTG 
CCGGTGACGG AGAAGGCCCT GGCCACGCAG GACATAGACG CCAATGGGGT CCCGGTCCTC 
ATCGGTCACG TAGACGACGC CACGTCCGAC GACCTGGCGT CGCTGCTCGA AGAGATCTTC 
AGGGTCACCA CCTCTGACTG TAATGATGCC AAGACATCCA AGGGTCCCCC GCACTGTGTG 
CTACTGCCAA CTTTCAAGGA TTCCAAGAAA TGTCTCGACC TGAACACCCT TAATGAGCTG 
GTCTTCGAGC GGCTTGTGTT GGACGACCCC CAGAACCACG TGGTCAGCAA GAGCAAGTAT 
GCCGACCAGC AGGCTCTGGC TGATGCCGGA GAAACCAGGG TGGTCTTCTA CCTCTACCAG 
TGCTTCTCGA GGCTCAACAA GGTGGCCGCC AAGCTGGAGA ATGAAGATTA CGAAACTATC 
GAGAAGACGA TTGTGTCGCA GGCGTCGACT TGCCTTTGCC ACTGTGAAAT CTACCCTGGC 
AAAAAGATAC CGCAGCAGGT CTTCTACCTA CTCCAGGAGT ATCACGCTGA AGGAGGTCAC 
CAAGACAGCC TGGAGTCTTT TCTGAAGAAA TGCGCAGACT GTATCAAGGG CCGCTTTGAT 
GCAGAGGAAG AGCAGGTTTC TCTGCTTGAA GTGCTCTCGC ATTCCTTCAA CTTGCTCAAG 
AAGAAATTCG GCAAGGTGTC TATCGCCAAC CGGGAACTGT TTGTGCTTAT CGACATCCTC 
AAGTACTTCA CCAAATCTGT TTCCATGGCA ATGGCACTCT TAGACTTCAG CACGCCTGCA 
GAGCTGGTTG ACCTACGCAG TTTCCAGGAG AGCCTGCTCG GTGCCCCCCT GTGCGTGTCC 
TGCCTGCCAC GCAAGGAAGC CGACCCCTTC GAGTTCTTCG AGAAGCCCTC TCAGGCGCTG 
ATGAACGAGC ATGCCATCAC CGAGAGCAAT CTGTGGCTGC CGCTAAAAGG TCTCGCAGTG 
GAGCTGCACA ACTTGTTTTA TTCCCTGCTT CGGCTTTCAC CAGAGACACG CAACAGGACC 
CTGACATGGA TAGGACACTG CATCACTGCA TGTTCAGAGC GGGGCAAACT GTGGAACAAC 
CAGGTGTCTG AGCTGTTCAT GGCAGTGCAA TCCGGCGACG GCTTTGCCCT GAACCTGGGG 
GCAGTGCTGC TGCGGCTGGC GCGGCCCTTC TCGGAGCCCT ACTCTCCCAA GCTGCTCAAG 
GTGCAGCACC AGTACTGCTC CTTCGAGCCA AAGTCGGAGC AGGAAGCCAA TGCCAGTTGC 
ATGCACATCA GAGGGCTGTC CAAGGAGACG TGCCTGGTGC CGAGGGAGGA GGGTGCGGCC 
AGCGAGTCCC CTTGCGGCCC CTTCAACTTC TCGACGGAGT GCTTCTTTGC CTGCCAACGC 
ATCCTGAGCC TGGGCTTCCG GGTGGTGCAC GAACGGCTGG CCCGGCTCAG CCAGGACCTG 
AACCGGGTGC GCAGGGTCTA CGAGGAGGCA CGCTCGCAGG GCGGCGACAG CTCTGAGGTC 
ACCCGCAGGC TGCAGGACAA TATGGAGAAG GGCATGACCC GGTTCCTGTC CCTGAAGGCG 
GTGCTCTTGG AGCCGGACTC CCTGGAGCAC ATGCTGCAGT TCCACGTGGC CTGCGCCACC 
TGGCTGTGCC ACCTGGCCAC GGCTGAGAGG CTGGACGCCT TCCAGCCCCT GTGCCTGCCT 
TTCCCCGAGC ACGGCAACGC CAGGCTCGCC TATGTTCCGG AGTTTGTCAT TGAGAACATC 
TGTGACTGCA TTGTGTTTGT CAAGCGCTTC AGCGAAAAAA GTCTGGCGCT CGTGGGCCCG 
AACTTGGAGC ACCTGATGAC GCTGATACTG CTGTTCATGG GCAGCCCCCA GAGAATGAAC 
AACCCGCACC TAAGAGCCAG GATGGCAGAG ATGTTGGAGG TGCTCATCAC CTCTGCCGAT 
GACGACCACT TCAGCGGCAT AATTCCCTCC ATGAACAGGG AGCGACTGTT CCGGAACCAC 
CCATTTGTAG CGGAGCTGCC TTCCACGCTG CTTCACGTCT TTGTGAGCAT CGAGATGACG 
GGCCAGAGCG TCACCTTTGA GCAGAAGTTC CACTACCGCC GTCCCATGTA CATTGTGTTG 
GACCACCTGT GGAACATGCC TGACCATCGG AACAAGATGA AGAGTCTGGC AGCTGAAGCA 
GAGGCCAACA TAGAATGTTC CAGTCCTCCA CTTTTCCTGA GATTCATTAA CCTTCTCATC 
AATGATGCCA TTTTTCTCTT GGACGAAGCC CTTTCGTACA TGAGCCGCCT GCGCGAGCTG 
CAGCAGGAAC GCCAGCAGCA GCAGCCTCTG GGGGGCCCTT TGTGGAGCCA GGGGGAGGCA 
AACATGCAGC ACGTGGGCAT GCTGGCCCAC TTCCACAACG TCATGGGCAC CGAGACCATC 
CGCACCCTGG CCTGGCTCAC CACCGAGATC AAGTCGCTCT TTTGCCACCC GACCATGGTG 
GACCGCATCG CAACCATGCT GAACTACTTC CTACTCCACC TGGTTGGACC CCAGAAGAAG 
AACCTGAAGG TGAAGGACTT CAGCGAGTAC GAGTTTAAGC CTCAGGAGCT GGTGCAGAAC 
ATCTGCAGGA TCTACACCAA CCTGGGCTGT GCCAACTCCG AGACCGCGCA GGCATTCTGC 
GTGGCCGTGT CCAGGGACGG CAGGTCATAC AGCCCTGAAC TGTTCCCCCA AGCACAGAGC 
GTCCTCTTGA AAATAGGCCA GAGCATTCTA TCCATTGGAG TCGGTGAACT AGCAGAAAAA 
ATTCTGAAAG CGGCGTCACA TCAGAAGCAA GAGGATGAAG CTATTGGAGA TGCGCCTGAA 
GAGTTCCTGG ACCCAATCAT GAGTACCATC ATGAACGACC CGGTGGTCCT GCCGAGTTCT 
GGCATCACCC TGGACCGTTC CACCATAGCA AGACATCTGC TCAGTGACCA GACTGACCCT 
TTCAACCGGT CTCCCCTGAC AATGGAGATG GTGACCTCGG ACAACGACCT CAAGAACAAG 
ATCCTGGAGT GGAGAGCAAA ACGAATGACC AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8038480 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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