IscW_ISCW009593 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	IscW_ISCW009593
Entrez Gene ID	8034391
Full Name	collagen alpha chain, type IV, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029993388.1	XP_029849248.1	collagen alpha-2(IV) chain
XM_002409077.1	XP_002409121.1	collagen alpha chain, type IV, putative

Pathways from BioSystems
Gene Ontology

The following IscW_ISCW009593 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IscW_ISCW009593 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIa16395	XM_029993388.1 Latest version!	Ixodes scapularis collagen alpha-2(IV) chain (LOC8034391), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IscW_ISCW009593 ( XM_029993388.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029849248.1 Ixodes scapularis collagen alpha-2(IV) chain (LOC8034391), mRNA.

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CloneID	OIa16395
Clone ID Related Accession (Same CDS sequence)	XM_029993388.1
Accession Version	XM_029993388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	collagen alpha-2(IV) chain
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021918015.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGTGGT CCCCCGCAGC TGGCAGGGAT CATAACAAAA GCCGACCACC TTCGTTGACC 
GTCGCCTTCT TCCTGGTCGC CCTGACTGTC CTGTTATGCC TACCGGACAG CGTCGACGCA 
GCCGCCTGCA AGGGATGTGC CATCCAGCCA TGCCTGTGCA AAGGTGAAAA AGGGGATGTG 
GGCTACCCTG GCCTGCCTGG CTCGATGGGC ACGGACGGAG CCCCCGGGGC CACGGGCCCC 
GATGGACCAC CTGGACCGAG AGGCGAGCGT GGTACCACGG GCGACACCGG GGACGCTGGC 
AGCAAGGGAA TCAGAGGTGA CGAAGGTGCC GTGGGGTTCC CAGGGCGGCC TGGAGCGCCC 
GGAAAACCCG GTTTGGATGG CGTTCATGGA GACCCAGGCA TAAATGGATG CAACGGAAGC 
AAGGGAGACG TTGGACCCAG AGGTTATGAC GGATATCCAG GACCAAGGGG ACCTGTTGGC 
CCTCAAGGGG ACCGTGGAGC GAAGGGTATA CCCGGTGATG GTGGCGTCAA CTCTATTGGT 
TATCGGGGAG AGCCAGGAGA CGATGGAAGA CCTGGCCCTC CTGGCGCACC GGGGCTTCCG 
GGCTATCACG GGGACAAAGG AGCCCGCGGT GAGACTGGAA AAGAGGGCAA CCGAGGTCCT 
CCCGGCGACC CCGGACCAAA GGGTGTACAA GGAGACGGCG TGCAAGGACC AAAGGGCACA 
AAGGGAGAAA TTGGACCCCA AGGTGAAGTC GGCGATCCCG GACAGTCGAC GTTTTTTACA 
ATGCCTCTTC CGACGGAACA AATTCCTGGT CAAAAAGGAC CCCAGGGAGA AAAGGGAGAA 
GTTGGAGAAT GGGGAGAACG AGGCTCACGT GGATTAGATG GGCGAGAAGG AATACCGGGT 
GACGACGGTC TCAAGGGAGA GAAAGGTGAC ATCGGCGAAG ATGGTCCACG TGGCAAACGA 
GGGAAGCCGG GACCAGAAGG CGATTTAGGA GACAAGGGCC AGAAGGGAGA ACCCGGATTC 
ACCGGACAAG ACGGAATGAT CGGTGTCAAG GGCTTTTCTG GAGACCCTGG AAGGCCCGGG 
AGACCTGGAC AGCAAGGACC ACCGGGACCA CCTGGGCCTT ACATTGAAAC TGCTGAAAGT 
GTGCCAGGAC CGAGAGGTCC GCAAGGAGAA CCAGGAGACC AAGGGCCGCC AGGATTTGAT 
GGAAACCCTG GACCACGCGG TCCTATGGGA TACCGGGGCC CTATAGGCGA GCCTGGGGTC 
CCTGGAGAGC AAGGTGAACC CGGACCTCGT GGTATTGCCA TCAAAGGCGA GCCGGGAGAA 
GACGGTTTCC CCGGCCTGCC CGGAGATCGG GGGCGTGAAG GACCTCAGGG GCCTCAGGGT 
CCCCCTGGGG AGAAGGGTCA GCAGGGCCTT AACGTCATGG GACCTCCCGG AGAAGACGGA 
AGGCCCGGAA GGGACGGAAG GCCCGGGCTT CCGGGAGTAC GCGGAGACCC CGGAGAACCA 
GGCGAGAAAG GAACCCCCGG TAGAGGCGTA CGACAGGTGG GCCCCGTCGG TCCCATGGGT 
CTTCCGGGAG AGCAGGGAGA GCCCGGCGCT CCAGGGCGTC CTGGAAGGGA CGGAGAGGAC 
GGCTTCCCTG GGCCCAGAGG CGACGACTGC GGCACCTGTC CACCCGGAAT CCCCGGAAAG 
CGCGGCGACC GAGGAGAGGA CGGCAGACCA GGATCGGAAG GCTCATCAGG ATTCCCTGGA 
AGTATGGGCC CACCAGGAAC CAAGGGAGAC TCCGGCTACC AGGGAAGGCC CGGTCTCCAA 
GGCTTCCCCG GCATTCCTGG ATCAGACGGC ATCGATGGGC TGAAGGGCGA GAAAGGTTTT 
CCCGGAGAAG TGATCATCGA CTACTATCCT CCAAACTCCA TTAAGGGAGA AAGGGGTGAT 
CCTGGTGCCG ACGGTCAACG AGGGAGTCCC GGTCTAGAGG GCCTGCCAGG CCCAGACGGA 
GCGCCGGGTA TCCCTGGTGT TGGCGGAGAG CCGGGCGACT ACGGAGATCG CGGAGCTCCT 
GGTTTTGCCG GCCAAGAGGG CTTCCCCGGT CTATCCGGAC AGAAGGGAGC GATGGGAGAC 
GGTTTTGACG GCACGAAGGG ACAGCGAGGT CCTCCGGGCT ACGACGGAGA GGTCGGAGTG 
CCGGGCCAAG TGGGAGAAAG AGGTGACCCC GGAACCCCCT TCACACCGGA GCAGTTATTG 
GAAGCGACCG GGCCTCCAGG CCCACCTGGT GAGGCTGGCC GAAACGGCCT CGAAGGGCCT 
CCAGGACCAC CGGGAAAACC TGGATTTGAT GGGCGTCCCG GAGTTCAAGG AGATAAGGGA 
GATCACGGAA GTCATGGCTA CTCTCAGCAA GGTCCGACAG GACTGAAAGG CTACCCCGGT 
CTTTCTGGTG ACCAGGGTCC TACAGGCAGA CCTGGGGGAA GAGGACGACC TGGTGAAATT 
GGTCTGGAAG GACGCCCAGG TCCAAAGGGT CAACGTGGTA TCCCTGGGAC AGGAAAAGGC 
ACCGGCCCGC CAGGCCCCCC GGGATTGCCT GGAAACAGAG GACAGCGTGG ATTTCCCGGC 
CCCGCGGGAG CTCCCGGTGT TGCGGGACCT CCGGGATACT CTGGTTCCAA GGGCCAGAAG 
GGAGAACCAG GCTACACGGG ATTTGATGGA CCCAAGGGCA TCAAGGGCGA CAGAGGAGAC 
GCTGGGCCAG GAACTTATGG TCTCCCTGGA CTGCCTGGTT ATGCCGGAGA CAAAGGTGAA 
AGAGGGGACA AGGGATCCCG TGGATCGGAT GGCTTTCCTG GCACTCCTGG CGATAAAGGG 
TTACCCGGAG ACGACGGGTT ACCCGGTGCA CGAGGTCGCG ACGGTATTCC AGGAAGGCAG 
GGACAGCCAG GAACCCCAGG AAGAGACGGG TTCCCGGGAA TACCTGGTGA GAAGGGAGAA 
ATTGGTGACC AGGGCCAACC AGGCCTTGAT GGATTCCCCG GTGAATCTGG TTTCCCCGGA 
TTCCCTGGAC TCAAAGGAGA CAAGGGCAAT GCAGGAAGGT CAGGGTTTCC AGGACTCCCC 
GGGAGGCCGG GAGCGGCGGG AGAACGTGGC AATGCAGGAT ATCCCGGTCT ACCTGGACAG 
AGGGGAGACA GGGCCACGCA AGGTGGTCGT GGAGATGATG GGGCACCGGG TGAAGAAGGA 
CTCGAGGGAC AACCCGGACA ACCGGGATTC CCTGGTCTTC CTGGTCCGAA GGGTCTTGCT 
GGAGAGCCTG GAAGAGCTCT TGATGCTCCA GAGGGTCCTA AAGGCGAGAA AGGACAGTCT 
GGGTACCCTG GACGAAATGG ACTACCAGGC TTCAAAGGAG AAATGGGTGA CATGGGAAGA 
GAAGGACCGA AGGGCATATC CGGTGAGGAA GGATTTCCTG GAGCCCCCGG ATTTGATGGT 
CGTCCTGGAG TACCTGGACT GCCTGGAATT GAAGGAGAAG ATGGAAGGCC AGGTTTTCCG 
GGCGTACCCG GAATCAAGGG TGACGAAGGT TTTCCCGGCC GCCCCGGTCT TCCCGGACCA 
CCTGGTCAGC CAGGACCGGC TGGGCCCGCT GGGCCTGATG GTCTTCCTGG ACTCAAAGGA 
GAACTCGGAG AGCCCGGATT CCCTGGCCTC CCAACGTCGG AGGTGAGCAA GGGCGACAGG 
GGAGAACCAG GAAGGCCAGG GATTCCGGGG CCGGCGGGTC TCCCCGGAGA CGACGGATTC 
CCAGGCCTCC CTGGACTCAA GGGGGCACTC GGAGAGCCGG GCGTGGATGG CTTTCCTGGC 
GCGCCTGGAA GACCCGGTCC TAAAGGATAT CCCGGAGGCC CTGGGCTTCC TGGATTAGAA 
GGTGCACCTG CCTTTGATAT ATACCCTGGT CTGAAGGGAG AGGAAGGTGA CCCCGGATAT 
CAGGGCCTGC CCGGAATTCC TGGCCCAAAA GGAGAGGCGG GAGATCCTGG TCAACCGGGT 
TACGATGGAC CCAAAGGCTA CCCCGGTTTC ACCACAATGG GCCCCAAGGG AGCACAAGGA 
GACGATGGTT TCCCAGGATC TCAAGGTCTC GATGGTAGGG ATGGCTCTCC TGGAGAGTCC 
GGCTTTCCAG GCCTGCCAGG AGTGAAAGGA GCCCGGGGTA ACCCTGGCTA CCCTGGTCCC 
TCCGTTCCTG GACTTCCGGG AATTGATGGT TTGAGGGGCA ACAACGGACA ATATGGTCCA 
AAGGGTGAAA GAGGCTATCC TGGAGAAATC GGACAGCCAG GATTTCCTGG AGTCAAGGGA 
GAACGTGGGG ACGACGGCCT TCCTGGAATT CCAGGCAAAC AAGGCGCTGC CGGTTTCCCC 
GGTGTCAAGG GAGAGCGCGG AGACCGTGCT CCAATGGTGG CGGAACTCCG GTTGAAAGGC 
GAGCAAGGAG ATCCTGGAGA AAATGGACTC GACGGCCCAC GGGGGCGGCC CGGAGAAGTG 
GGCCTGCCCG GTAGGGACGG CTTTCCCGGA GAATCGGGAG ACATCGGTCC CCCCGGGTTC 
CCGGGCCCCC CGGGATACTT CGGTCCGAAG GGACTTCCAG GGGACGACGG AGACAATGGT 
CTTCCGGGAT ACCCTGGAAC AGTCGGAACA CCAGGTCGCC CTGGCAACAG AGGACCGCCA 
GGAGGTCCGG CCAAATCTCG GGGGTACTTC TTCACGAGGC ACAGCCAGAC ATCGCGGGTT 
CCGCAGTGCC CGAGGGGAAC GTACAACCTC TGGGAAGGCT ACTCTCTCTT GTACATCCTA 
GGAGATGAGA AAGCGCACGG ACAAGACTTG GGAAGCGCCG GTAGCTGTCT TCGGCGCTTC 
AGTACCATGC CGTTCCTGTT CTGCAGTTTG AACAATGTGT GCAATTTTGC TTCGAGAAAC 
GACTACAGCT ACTGGCTGAG CACTCCTGAG CCGATGCCGA TGGCAATGAC GCCAATCGTG 
GGAAGAGATA TCCAGAAATT CATCAGCCGA TGCAGTGTCT GCGAGTCCCC CACTCAGGTC 
ATAGCCATAC ACAGCCAAAC TCTGGAAGTG CCAGAGTGTC CTCAAGGCTG GGATGGCATT 
TGGACTGGAT ACAGTTTCTT TATGAACACC GATTCCGGAG CAGAAGGCAG CGGCCAGCCC 
CTGGTGTCTC CGGGCTCATG CCTCGAGGAC TTCAGGCCGA ATCCTTTCAT TGAGTGCCAC 
GGTCACGGAC GGTGCAACTA CTACACGACC GCGTACAGCT ACTGGCTGGC CACTATCGAG 
CGGAACGACA TGTTCCGCCC TCCGGACCCG CAAACGCTCA AGGCGGGCGA CCTCCGCACC 
CGCGTCTCTC GCTGCCAGGT CTGCAGGAGG CGGGTCTACG TTCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029849248.1
CDS	308..5635
Translation	MRWSPAAGRDHNKSRPPSLTVAFFLVALTVLLCLPDSVDAAACKGCAIQPCLCKGEKGDVGYPGLPGSMGTDGAPGATGPDGPPGPRGERGTTGDTGDAGSKGIRGDEGAVGFPGRPGAPGKPGLDGVHGDPGINGCNGSKGDVGPRGYDGYPGPRGPVGPQGDRGAKGIPGDGGVNSIGYRGEPGDDGRPGPPGAPGLPGYHGDKGARGETGKEGNRGPPGDPGPKGVQGDGVQGPKGTKGEIGPQGEVGDPGQSTFFTMPLPTEQIPGQKGPQGEKGEVGEWGERGSRGLDGREGIPGDDGLKGEKGDIGEDGPRGKRGKPGPEGDLGDKGQKGEPGFTGQDGMIGVKGFSGDPGRPGRPGQQGPPGPPGPYIETAESVPGPRGPQGEPGDQGPPGFDGNPGPRGPMGYRGPIGEPGVPGEQGEPGPRGIAIKGEPGEDGFPGLPGDRGREGPQGPQGPPGEKGQQGLNVMGPPGEDGRPGRDGRPGLPGVRGDPGEPGEKGTPGRGVRQVGPVGPMGLPGEQGEPGAPGRPGRDGEDGFPGPRGDDCGTCPPGIPGKRGDRGEDGRPGSEGSSGFPGSMGPPGTKGDSGYQGRPGLQGFPGIPGSDGIDGLKGEKGFPGEVIIDYYPPNSIKGERGDPGADGQRGSPGLEGLPGPDGAPGIPGVGGEPGDYGDRGAPGFAGQEGFPGLSGQKGAMGDGFDGTKGQRGPPGYDGEVGVPGQVGERGDPGTPFTPEQLLEATGPPGPPGEAGRNGLEGPPGPPGKPGFDGRPGVQGDKGDHGSHGYSQQGPTGLKGYPGLSGDQGPTGRPGGRGRPGEIGLEGRPGPKGQRGIPGTGKGTGPPGPPGLPGNRGQRGFPGPAGAPGVAGPPGYSGSKGQKGEPGYTGFDGPKGIKGDRGDAGPGTYGLPGLPGYAGDKGERGDKGSRGSDGFPGTPGDKGLPGDDGLPGARGRDGIPGRQGQPGTPGRDGFPGIPGEKGEIGDQGQPGLDGFPGESGFPGFPGLKGDKGNAGRSGFPGLPGRPGAAGERGNAGYPGLPGQRGDRATQGGRGDDGAPGEEGLEGQPGQPGFPGLPGPKGLAGEPGRALDAPEGPKGEKGQSGYPGRNGLPGFKGEMGDMGREGPKGISGEEGFPGAPGFDGRPGVPGLPGIEGEDGRPGFPGVPGIKGDEGFPGRPGLPGPPGQPGPAGPAGPDGLPGLKGELGEPGFPGLPTSEVSKGDRGEPGRPGIPGPAGLPGDDGFPGLPGLKGALGEPGVDGFPGAPGRPGPKGYPGGPGLPGLEGAPAFDIYPGLKGEEGDPGYQGLPGIPGPKGEAGDPGQPGYDGPKGYPGFTTMGPKGAQGDDGFPGSQGLDGRDGSPGESGFPGLPGVKGARGNPGYPGPSVPGLPGIDGLRGNNGQYGPKGERGYPGEIGQPGFPGVKGERGDDGLPGIPGKQGAAGFPGVKGERGDRAPMVAELRLKGEQGDPGENGLDGPRGRPGEVGLPGRDGFPGESGDIGPPGFPGPPGYFGPKGLPGDDGDNGLPGYPGTVGTPGRPGNRGPPGGPAKSRGYFFTRHSQTSRVPQCPRGTYNLWEGYSLLYILGDEKAHGQDLGSAGSCLRRFSTMPFLFCSLNNVCNFASRNDYSYWLSTPEPMPMAMTPIVGRDIQKFISRCSVCESPTQVIAIHSQTLEVPECPQGWDGIWTGYSFFMNTDSGAEGSGQPLVSPGSCLEDFRPNPFIECHGHGRCNYYTTAYSYWLATIERNDMFRPPDPQTLKAGDLRTRVSRCQVCRRRVYVP

Target ORF information:

RefSeq Version	XM_029993388.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis collagen alpha-2(IV) chain (LOC8034391), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029993388.1

ORF Insert Sequence:

ATGCGGTGGT CCCCCGCAGC TGGCAGGGAT CATAACAAAA GCCGACCACC TTCGTTGACC 
GTCGCCTTCT TCCTGGTCGC CCTGACTGTC CTGTTATGCC TACCGGACAG CGTCGACGCA 
GCCGCCTGCA AGGGATGTGC CATCCAGCCA TGCCTGTGCA AAGGTGAAAA AGGGGATGTG 
GGCTACCCTG GCCTGCCTGG CTCGATGGGC ACGGACGGAG CCCCCGGGGC CACGGGCCCC 
GATGGACCAC CTGGACCGAG AGGCGAGCGT GGTACCACGG GCGACACCGG GGACGCTGGC 
AGCAAGGGAA TCAGAGGTGA CGAAGGTGCC GTGGGGTTCC CAGGGCGGCC TGGAGCGCCC 
GGAAAACCCG GTTTGGATGG CGTTCATGGA GACCCAGGCA TAAATGGATG CAACGGAAGC 
AAGGGAGACG TTGGACCCAG AGGTTATGAC GGATATCCAG GACCAAGGGG ACCTGTTGGC 
CCTCAAGGGG ACCGTGGAGC GAAGGGTATA CCCGGTGATG GTGGCGTCAA CTCTATTGGT 
TATCGGGGAG AGCCAGGAGA CGATGGAAGA CCTGGCCCTC CTGGCGCACC GGGGCTTCCG 
GGCTATCACG GGGACAAAGG AGCCCGCGGT GAGACTGGAA AAGAGGGCAA CCGAGGTCCT 
CCCGGCGACC CCGGACCAAA GGGTGTACAA GGAGACGGCG TGCAAGGACC AAAGGGCACA 
AAGGGAGAAA TTGGACCCCA AGGTGAAGTC GGCGATCCCG GACAGTCGAC GTTTTTTACA 
ATGCCTCTTC CGACGGAACA AATTCCTGGT CAAAAAGGAC CCCAGGGAGA AAAGGGAGAA 
GTTGGAGAAT GGGGAGAACG AGGCTCACGT GGATTAGATG GGCGAGAAGG AATACCGGGT 
GACGACGGTC TCAAGGGAGA GAAAGGTGAC ATCGGCGAAG ATGGTCCACG TGGCAAACGA 
GGGAAGCCGG GACCAGAAGG CGATTTAGGA GACAAGGGCC AGAAGGGAGA ACCCGGATTC 
ACCGGACAAG ACGGAATGAT CGGTGTCAAG GGCTTTTCTG GAGACCCTGG AAGGCCCGGG 
AGACCTGGAC AGCAAGGACC ACCGGGACCA CCTGGGCCTT ACATTGAAAC TGCTGAAAGT 
GTGCCAGGAC CGAGAGGTCC GCAAGGAGAA CCAGGAGACC AAGGGCCGCC AGGATTTGAT 
GGAAACCCTG GACCACGCGG TCCTATGGGA TACCGGGGCC CTATAGGCGA GCCTGGGGTC 
CCTGGAGAGC AAGGTGAACC CGGACCTCGT GGTATTGCCA TCAAAGGCGA GCCGGGAGAA 
GACGGTTTCC CCGGCCTGCC CGGAGATCGG GGGCGTGAAG GACCTCAGGG GCCTCAGGGT 
CCCCCTGGGG AGAAGGGTCA GCAGGGCCTT AACGTCATGG GACCTCCCGG AGAAGACGGA 
AGGCCCGGAA GGGACGGAAG GCCCGGGCTT CCGGGAGTAC GCGGAGACCC CGGAGAACCA 
GGCGAGAAAG GAACCCCCGG TAGAGGCGTA CGACAGGTGG GCCCCGTCGG TCCCATGGGT 
CTTCCGGGAG AGCAGGGAGA GCCCGGCGCT CCAGGGCGTC CTGGAAGGGA CGGAGAGGAC 
GGCTTCCCTG GGCCCAGAGG CGACGACTGC GGCACCTGTC CACCCGGAAT CCCCGGAAAG 
CGCGGCGACC GAGGAGAGGA CGGCAGACCA GGATCGGAAG GCTCATCAGG ATTCCCTGGA 
AGTATGGGCC CACCAGGAAC CAAGGGAGAC TCCGGCTACC AGGGAAGGCC CGGTCTCCAA 
GGCTTCCCCG GCATTCCTGG ATCAGACGGC ATCGATGGGC TGAAGGGCGA GAAAGGTTTT 
CCCGGAGAAG TGATCATCGA CTACTATCCT CCAAACTCCA TTAAGGGAGA AAGGGGTGAT 
CCTGGTGCCG ACGGTCAACG AGGGAGTCCC GGTCTAGAGG GCCTGCCAGG CCCAGACGGA 
GCGCCGGGTA TCCCTGGTGT TGGCGGAGAG CCGGGCGACT ACGGAGATCG CGGAGCTCCT 
GGTTTTGCCG GCCAAGAGGG CTTCCCCGGT CTATCCGGAC AGAAGGGAGC GATGGGAGAC 
GGTTTTGACG GCACGAAGGG ACAGCGAGGT CCTCCGGGCT ACGACGGAGA GGTCGGAGTG 
CCGGGCCAAG TGGGAGAAAG AGGTGACCCC GGAACCCCCT TCACACCGGA GCAGTTATTG 
GAAGCGACCG GGCCTCCAGG CCCACCTGGT GAGGCTGGCC GAAACGGCCT CGAAGGGCCT 
CCAGGACCAC CGGGAAAACC TGGATTTGAT GGGCGTCCCG GAGTTCAAGG AGATAAGGGA 
GATCACGGAA GTCATGGCTA CTCTCAGCAA GGTCCGACAG GACTGAAAGG CTACCCCGGT 
CTTTCTGGTG ACCAGGGTCC TACAGGCAGA CCTGGGGGAA GAGGACGACC TGGTGAAATT 
GGTCTGGAAG GACGCCCAGG TCCAAAGGGT CAACGTGGTA TCCCTGGGAC AGGAAAAGGC 
ACCGGCCCGC CAGGCCCCCC GGGATTGCCT GGAAACAGAG GACAGCGTGG ATTTCCCGGC 
CCCGCGGGAG CTCCCGGTGT TGCGGGACCT CCGGGATACT CTGGTTCCAA GGGCCAGAAG 
GGAGAACCAG GCTACACGGG ATTTGATGGA CCCAAGGGCA TCAAGGGCGA CAGAGGAGAC 
GCTGGGCCAG GAACTTATGG TCTCCCTGGA CTGCCTGGTT ATGCCGGAGA CAAAGGTGAA 
AGAGGGGACA AGGGATCCCG TGGATCGGAT GGCTTTCCTG GCACTCCTGG CGATAAAGGG 
TTACCCGGAG ACGACGGGTT ACCCGGTGCA CGAGGTCGCG ACGGTATTCC AGGAAGGCAG 
GGACAGCCAG GAACCCCAGG AAGAGACGGG TTCCCGGGAA TACCTGGTGA GAAGGGAGAA 
ATTGGTGACC AGGGCCAACC AGGCCTTGAT GGATTCCCCG GTGAATCTGG TTTCCCCGGA 
TTCCCTGGAC TCAAAGGAGA CAAGGGCAAT GCAGGAAGGT CAGGGTTTCC AGGACTCCCC 
GGGAGGCCGG GAGCGGCGGG AGAACGTGGC AATGCAGGAT ATCCCGGTCT ACCTGGACAG 
AGGGGAGACA GGGCCACGCA AGGTGGTCGT GGAGATGATG GGGCACCGGG TGAAGAAGGA 
CTCGAGGGAC AACCCGGACA ACCGGGATTC CCTGGTCTTC CTGGTCCGAA GGGTCTTGCT 
GGAGAGCCTG GAAGAGCTCT TGATGCTCCA GAGGGTCCTA AAGGCGAGAA AGGACAGTCT 
GGGTACCCTG GACGAAATGG ACTACCAGGC TTCAAAGGAG AAATGGGTGA CATGGGAAGA 
GAAGGACCGA AGGGCATATC CGGTGAGGAA GGATTTCCTG GAGCCCCCGG ATTTGATGGT 
CGTCCTGGAG TACCTGGACT GCCTGGAATT GAAGGAGAAG ATGGAAGGCC AGGTTTTCCG 
GGCGTACCCG GAATCAAGGG TGACGAAGGT TTTCCCGGCC GCCCCGGTCT TCCCGGACCA 
CCTGGTCAGC CAGGACCGGC TGGGCCCGCT GGGCCTGATG GTCTTCCTGG ACTCAAAGGA 
GAACTCGGAG AGCCCGGATT CCCTGGCCTC CCAACGTCGG AGGTGAGCAA GGGCGACAGG 
GGAGAACCAG GAAGGCCAGG GATTCCGGGG CCGGCGGGTC TCCCCGGAGA CGACGGATTC 
CCAGGCCTCC CTGGACTCAA GGGGGCACTC GGAGAGCCGG GCGTGGATGG CTTTCCTGGC 
GCGCCTGGAA GACCCGGTCC TAAAGGATAT CCCGGAGGCC CTGGGCTTCC TGGATTAGAA 
GGTGCACCTG CCTTTGATAT ATACCCTGGT CTGAAGGGAG AGGAAGGTGA CCCCGGATAT 
CAGGGCCTGC CCGGAATTCC TGGCCCAAAA GGAGAGGCGG GAGATCCTGG TCAACCGGGT 
TACGATGGAC CCAAAGGCTA CCCCGGTTTC ACCACAATGG GCCCCAAGGG AGCACAAGGA 
GACGATGGTT TCCCAGGATC TCAAGGTCTC GATGGTAGGG ATGGCTCTCC TGGAGAGTCC 
GGCTTTCCAG GCCTGCCAGG AGTGAAAGGA GCCCGGGGTA ACCCTGGCTA CCCTGGTCCC 
TCCGTTCCTG GACTTCCGGG AATTGATGGT TTGAGGGGCA ACAACGGACA ATATGGTCCA 
AAGGGTGAAA GAGGCTATCC TGGAGAAATC GGACAGCCAG GATTTCCTGG AGTCAAGGGA 
GAACGTGGGG ACGACGGCCT TCCTGGAATT CCAGGCAAAC AAGGCGCTGC CGGTTTCCCC 
GGTGTCAAGG GAGAGCGCGG AGACCGTGCT CCAATGGTGG CGGAACTCCG GTTGAAAGGC 
GAGCAAGGAG ATCCTGGAGA AAATGGACTC GACGGCCCAC GGGGGCGGCC CGGAGAAGTG 
GGCCTGCCCG GTAGGGACGG CTTTCCCGGA GAATCGGGAG ACATCGGTCC CCCCGGGTTC 
CCGGGCCCCC CGGGATACTT CGGTCCGAAG GGACTTCCAG GGGACGACGG AGACAATGGT 
CTTCCGGGAT ACCCTGGAAC AGTCGGAACA CCAGGTCGCC CTGGCAACAG AGGACCGCCA 
GGAGGTCCGG CCAAATCTCG GGGGTACTTC TTCACGAGGC ACAGCCAGAC ATCGCGGGTT 
CCGCAGTGCC CGAGGGGAAC GTACAACCTC TGGGAAGGCT ACTCTCTCTT GTACATCCTA 
GGAGATGAGA AAGCGCACGG ACAAGACTTG GGAAGCGCCG GTAGCTGTCT TCGGCGCTTC 
AGTACCATGC CGTTCCTGTT CTGCAGTTTG AACAATGTGT GCAATTTTGC TTCGAGAAAC 
GACTACAGCT ACTGGCTGAG CACTCCTGAG CCGATGCCGA TGGCAATGAC GCCAATCGTG 
GGAAGAGATA TCCAGAAATT CATCAGCCGA TGCAGTGTCT GCGAGTCCCC CACTCAGGTC 
ATAGCCATAC ACAGCCAAAC TCTGGAAGTG CCAGAGTGTC CTCAAGGCTG GGATGGCATT 
TGGACTGGAT ACAGTTTCTT TATGAACACC GATTCCGGAG CAGAAGGCAG CGGCCAGCCC 
CTGGTGTCTC CGGGCTCATG CCTCGAGGAC TTCAGGCCGA ATCCTTTCAT TGAGTGCCAC 
GGTCACGGAC GGTGCAACTA CTACACGACC GCGTACAGCT ACTGGCTGGC CACTATCGAG 
CGGAACGACA TGTTCCGCCC TCCGGACCCG CAAACGCTCA AGGCGGGCGA CCTCCGCACC 
CGCGTCTCTC GCTGCCAGGT CTGCAGGAGG CGGGTCTACG TTCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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IscW_ISCW009593 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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