LOC8029916 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8029916
Entrez Gene ID	8029916
Full Name	tho2 protein, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002408201.1	XP_002408245.1	tho2 protein, putative
XM_029987809.1	XP_029843669.1	THO complex subunit 2 isoform X2
XM_029987812.1	XP_029843672.1	THO complex subunit 2 isoform X5
XM_029987808.1	XP_029843668.1	THO complex subunit 2 isoform X1
XM_029987811.1	XP_029843671.1	THO complex subunit 2 isoform X4
XM_029987813.1	XP_029843673.1	THO complex subunit 2 isoform X6
XM_029987810.1	XP_029843670.1	THO complex subunit 2 isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC8029916 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8029916 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OIa03822	XM_002408201.1 Latest version!	Ixodes scapularis tho2 protein, putative, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OIa29771	XM_029987813.1 Latest version!	Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OIa29766	XM_029987808.1 Latest version!	Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIa29767	XM_029987809.1 Latest version!	Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIa29768	XM_029987810.1 Latest version!	Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIa29769	XM_029987811.1 Latest version!	Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OIa29770	XM_029987812.1 Latest version!	Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8029916 ( XM_002408201.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002408245.1 Ixodes scapularis tho2 protein, putative, mRNA.

Price & Availability↑

CloneID	OIa03822
Clone ID Related Accession (Same CDS sequence)	XM_002408201.1
Accession Version	XM_002408201.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1245600000000
Organism	Ixodes scapularis(black-legged tick)
Product	tho2 protein, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002620414). COMPLETENESS: incomplete on both ends.

ATGCAGCTTC TGTTACACGA CCTCTGCTGG CACGTGCTGG AGGATCGCCT GAAAGTCGAG 
CAAGCTGTGT CGGCACTGGC CGAAGTGACT GCCTTGCATC AGGAGCTGCC CTCGATGCTG 
GCCGACGTCA TCTTTCTGCT GGACATGGAG ACGCTGTCGG TGGAGAACCG TGACCAGAGG 
GACCGTTTTT ACTGGCTCGT CGGCGGCTGT GCAAAGGTTG TTCCCGACTC GCTGCTGAAG 
GAGCGGCTGG ACATCGAGAC CTTGGGAGAC GGCGGGATCG TGAAGAACAG CCGTGTCATG 
CTCACCAAGT TTGTCAAGAT CAAGACGCGA CTGTTCTACA AGCAGAACAA GTACAACCTG 
TTCCGGGAGG AGAGCGAGGG CTACGCCAAG CTGACCACGG AGCTGAGCCA GGAGATCACC 
AGCAAGATCA GCCCCGATTA CATGATAGAA GTGATGCGCT CGCTCATTGG CTGCTTCAAT 
TTGGACCCCA ACCGCGTGTT GGACGTGGTT CTGGAGGCGT TTGAGTGCCG ACTCCATCTG 
GAGGACTTCT TTGTCCCTCT GCTGACAAAG TTCCTTCGCA ACCCTGCCAC CATAAGCCAG 
GTGCTGGGCT TCAAGTTCAC CTTCTACCAG CTGCTGCCCG ACGACGGGGC CCTGTCGAGG 
CAGCACAAGA AGGAGGTGGC GGCGGCTAAG ACGCACAGCG GGCTGCCGGA GGGCTTCCGG 
CGCTCGTACG CGTCGTCCCG TCGGGGGGGC CTGCGGCCGC CCGTCCTCGT GGAGACCTTC 
GGGGACTTCC AGGCGCGCGC CCTGCCCATG CTCGTGGCCC TGGGCCCCAT GCTGCACCTG 
GACCCGGTGC TCATCGTCAA GGTGGGAGCC CTCGGCCGCC CTGAGATGCA TCGACGTGCC 
GCATCTCGGG GCGCAATTAG CGAAAGTGCG GCGCCCGCAA CGCGTCTCGA CCGACTGGCC 
TCGCGCTGTT TAAACACGGA CGTCAAGTTA GTTTTTTTGT TCACGGCCAA GGTGGTGCGC 
CTGGCCAGAG CGCACCTGCA CAAGACCAGC TCCTCGGCGG CTGGAGACCT GCGGTTCGAC 
TTGCTCACCC TGGTCGGGGA GAGCCTGCTG CCCTCCCTGT CCCTTCTCGA CTGCAACTGC 
TGCGTGGCCG ACGAGATCTG GTCCCTGCTC AAGCTCTACC CCTACCAGCA GAGGTACCGT 
CTGTACAGCC AGTGGAAGAA CGAGGCGTAC GTGAGTCACC CGTTGCTCAT TCGGGTGAAG 
GCGGACTCGC TCAAGAGGAT CAAGTACATC ATGAAGAGAC TCAGCAAGGA GAATGTGAAG 
CCTTCGGGGC GACAGATTGG CAAGCTCAGC CACAGCAACC CTTGCTTTCT CTTTGACTAT 
ATCCTGTCCC AGATCCAGAC CTGGGACAAC CTGATTGTGC CCGTGGTGGA CTCCCTCAAG 
TACCTCACCA TGCTGTCCTA CGACGTTCTC GCCTACTGCG TGGCGGAGGC GCTCTGCAAT 
CCGGAGAAGG ACCGCATGAA GCACGACGGC ACCAGCATCT CCCTCTGGCT GCAGAGTCTG 
GCCAGTTTCT GTGGCGCCGT CTTCAAGAAG TACTCCATCG ACCTGACGGG TCTGCTGCAG 
CTCGTGGCAA ATCAGCTGAA GGCAGAAAAA AGCCTTGACC TGCTGGTGCT GAAGGACATC 
GTACAGAAGA TGACCGGCAT CGAGTCGACG GAACAGGCAA CGCAGGACCA ACTGGAGGCG 
ATGTGCGGAG GCGAACTGCT TAAGGCCGAG GGTGGCTACT TCCACCAGCT GCGCAACACC 
AAGAAGTCGT CCCAGCGGCT GAAGGAGGCC CTGCTGGAGC AGGACCTGGC CCTGCCGCTC 
TGCCTGCTCA TGGCCCAGCA GAAGAACTGC ATCCTCTACC GGGACCAGGA GGCCTCCCAC 
CTCAAGCTGG TTGGAAAGCT CTACGACCAG TGCCAGGACA CCCTGGTCCA GTTTGGCAGC 
TTCCTGTCGA GCAGCCTGAG CACCGAAGAG TACGCCGGCC GCCTGCCGCC CGTGGACCAG 
CTGCTCTCGC GGTTCCACGT GCAGGCCGAC GTGGCCTTCT TTTTGGCCAG GCCCATGTTC 
GGACATTCCA TCGCGCTCAA GTTCGACGAC GCCAAGAGGA GGGACAAGAC GTTCAAAAGT 
CTAACCGCCG CGCAGAAGTT GCAGCGCTAC GTCGACGCGG TGGACCAGGT GATGTCGCGG 
GTCGTCGAGA GTGTGCGGCC GCTGCACCCG TCCAAGACGT GGGAGGACCT CAGCCCGCAG 
TTCTACGTCA CCTTCTGGTC GCTCTCGATG TACGACCTCC ACGTGCCCAC ATCCAGCTAC 
GAGAGGGAGG TGGGCAAGTT GAAGCAGCAG GTGGTGCAGG CGGAGGACAG CAAGGACATG 
GTGCCCAGCA AGCGCAAGAA GGAGCGTGAC CGGTGCGAAG CGCTCATGGA GAAGCTGCAG 
GAGGAGGAGC GGAAGCAGCA GGACCACTGC GGGCGCGTCC TGGCACGGCT GCGCAGCGAG 
AAGGACGCCT GGTTCCACTC CCGTTCGGCC AAGAACGAGA CCATCACGCA GTTCCTGCAG 
CTGTGCGTGT TCCCGCGCTG CACGTTCACG GCCCTGGACG CCCTCTACTG CGCCAAGTTT 
GTGCACCTGA TCCACATCCT GAAGACGCCA AACTTCTCCA CCCTCATCTG CTACGACAAG 
ATCTTTTGCG ACATCACTTA CACGGTGACG GCATGCACGG AGAACGAAGC GAACCGCTAC 
GGGCGCTTCC TCTGCTCCAT GCTGGAGACG GTGATGCGGT GGCACAGCGA CAAGGCCATC 
TTTGACAAGG AGTGTGCCAA CTTCCCCGGC TTCATGACCA AGTTCCGGGG CGCCAGCCAG 
TCGTCGGACA TTGCCACGGA CCACGTGGAC TACGAGAACT ACCGGCACGT CTGTCACAAG 
TGGCACTTCA AGATCACTAA GGCCCTGGTC ATCTGTCTGG AGTCTGGGGA CTACGTCCAG 
ATCCGGAACA GCCTGATTGT GCTCACCAAG GTGCTGCCCC ACTTCCCGGT CATGACCAAC 
TTGGGCCAGG CGCTGGAGCG GCGCATCGAG AAGATCCGCC AGGAGGAGAA GGAGAAGAGG 
CCGGACCTCT TTGCTCTTGC CACCGGCTAC GCCGGCCAGC TGAAGGGCAA GAAATCCGAG 
CTGATCCCAG AGCACGAGTT CCACCTCAAG GACAGCAAAG ACGGCCGCGC GGTGAGCGCC 
ACCGCACAGG CGAAGAGCGC CGCAGATCAC ACGGTGACGA CGGCGGCACG CGCCGCCACC 
TCTTCCCAAG TGGCGAGTGT TGATGGTGCC AAGGAGAAGC GGGGAGACTC CGTGAGGGAG 
TCCAACTCGT CACCAGCACT GTCCGGCAAT TCTTCCGGGA AGCTGTCGAG CAGCAGCGGA 
GGTCTCGTCC AGTCTTCGTC GTCGTCGTCG TCGTCGTCCA AGATGACATC GTCCAGTAGT 
TCTTCCGCCA CCCGCAGGGA GTCCAGCAGG TCGCTGAGCA AGGAGTCCGG GAGCGGGGAC 
GGACACGACA AGGGCGCCGC CACCGACCGC AAGGGCAGCT CGCTGTCCTC CCTCCACGAG 
CAGGACGTCA GGCCCTCCAA GGACGACATC CACAAAAATG ACCACTGGCT GGAGCTCGGG 
GACGGCAGTC CCCGGGGCCG TAGCCTCGGG GGCACGCAGA GCCCCAAGGT CTCGGAACCC 
GAGGGGAAGT CTGAGGACCG CGGGGGAGGG GGGGACCGCC CTTCTGAGAA GTGGGCTCAC 
CCCAAGGACT CCCACGACCC GGCCACGGGG GACAAGGGCA AGGCCAAGAA GGGCAGCCGC 
AAGCGGGACC ACTCCGACGA GCCCCCGCTC GAGGGCAAGC GCAAGAAGGA CGAGGACGCC 
TGTGAGTGCA GGGGGGACCA CGGGCCCCGG TTGGAGTGGG TTTGGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002408245.1
CDS	1..3951
Translation	MQLLLHDLCWHVLEDRLKVEQAVSALAEVTALHQELPSMLADVIFLLDMETLSVENRDQRDRFYWLVGGCAKVVPDSLLKERLDIETLGDGGIVKNSRVMLTKFVKIKTRLFYKQNKYNLFREESEGYAKLTTELSQEITSKISPDYMIEVMRSLIGCFNLDPNRVLDVVLEAFECRLHLEDFFVPLLTKFLRNPATISQVLGFKFTFYQLLPDDGALSRQHKKEVAAAKTHSGLPEGFRRSYASSRRGGLRPPVLVETFGDFQARALPMLVALGPMLHLDPVLIVKVGALGRPEMHRRAASRGAISESAAPATRLDRLASRCLNTDVKLVFLFTAKVVRLARAHLHKTSSSAAGDLRFDLLTLVGESLLPSLSLLDCNCCVADEIWSLLKLYPYQQRYRLYSQWKNEAYVSHPLLIRVKADSLKRIKYIMKRLSKENVKPSGRQIGKLSHSNPCFLFDYILSQIQTWDNLIVPVVDSLKYLTMLSYDVLAYCVAEALCNPEKDRMKHDGTSISLWLQSLASFCGAVFKKYSIDLTGLLQLVANQLKAEKSLDLLVLKDIVQKMTGIESTEQATQDQLEAMCGGELLKAEGGYFHQLRNTKKSSQRLKEALLEQDLALPLCLLMAQQKNCILYRDQEASHLKLVGKLYDQCQDTLVQFGSFLSSSLSTEEYAGRLPPVDQLLSRFHVQADVAFFLARPMFGHSIALKFDDAKRRDKTFKSLTAAQKLQRYVDAVDQVMSRVVESVRPLHPSKTWEDLSPQFYVTFWSLSMYDLHVPTSSYEREVGKLKQQVVQAEDSKDMVPSKRKKERDRCEALMEKLQEEERKQQDHCGRVLARLRSEKDAWFHSRSAKNETITQFLQLCVFPRCTFTALDALYCAKFVHLIHILKTPNFSTLICYDKIFCDITYTVTACTENEANRYGRFLCSMLETVMRWHSDKAIFDKECANFPGFMTKFRGASQSSDIATDHVDYENYRHVCHKWHFKITKALVICLESGDYVQIRNSLIVLTKVLPHFPVMTNLGQALERRIEKIRQEEKEKRPDLFALATGYAGQLKGKKSELIPEHEFHLKDSKDGRAVSATAQAKSAADHTVTTAARAATSSQVASVDGAKEKRGDSVRESNSSPALSGNSSGKLSSSSGGLVQSSSSSSSSSKMTSSSSSSATRRESSRSLSKESGSGDGHDKGAATDRKGSSLSSLHEQDVRPSKDDIHKNDHWLELGDGSPRGRSLGGTQSPKVSEPEGKSEDRGGGGDRPSEKWAHPKDSHDPATGDKGKAKKGSRKRDHSDEPPLEGKRKKDEDACECRGDHGPRLEWVWL

Target ORF information:

RefSeq Version	XM_002408201.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis tho2 protein, putative, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002408201.1

ORF Insert Sequence:

ATGCAGCTTC TGTTACACGA CCTCTGCTGG CACGTGCTGG AGGATCGCCT GAAAGTCGAG 
CAAGCTGTGT CGGCACTGGC CGAAGTGACT GCCTTGCATC AGGAGCTGCC CTCGATGCTG 
GCCGACGTCA TCTTTCTGCT GGACATGGAG ACGCTGTCGG TGGAGAACCG TGACCAGAGG 
GACCGTTTTT ACTGGCTCGT CGGCGGCTGT GCAAAGGTTG TTCCCGACTC GCTGCTGAAG 
GAGCGGCTGG ACATCGAGAC CTTGGGAGAC GGCGGGATCG TGAAGAACAG CCGTGTCATG 
CTCACCAAGT TTGTCAAGAT CAAGACGCGA CTGTTCTACA AGCAGAACAA GTACAACCTG 
TTCCGGGAGG AGAGCGAGGG CTACGCCAAG CTGACCACGG AGCTGAGCCA GGAGATCACC 
AGCAAGATCA GCCCCGATTA CATGATAGAA GTGATGCGCT CGCTCATTGG CTGCTTCAAT 
TTGGACCCCA ACCGCGTGTT GGACGTGGTT CTGGAGGCGT TTGAGTGCCG ACTCCATCTG 
GAGGACTTCT TTGTCCCTCT GCTGACAAAG TTCCTTCGCA ACCCTGCCAC CATAAGCCAG 
GTGCTGGGCT TCAAGTTCAC CTTCTACCAG CTGCTGCCCG ACGACGGGGC CCTGTCGAGG 
CAGCACAAGA AGGAGGTGGC GGCGGCTAAG ACGCACAGCG GGCTGCCGGA GGGCTTCCGG 
CGCTCGTACG CGTCGTCCCG TCGGGGGGGC CTGCGGCCGC CCGTCCTCGT GGAGACCTTC 
GGGGACTTCC AGGCGCGCGC CCTGCCCATG CTCGTGGCCC TGGGCCCCAT GCTGCACCTG 
GACCCGGTGC TCATCGTCAA GGTGGGAGCC CTCGGCCGCC CTGAGATGCA TCGACGTGCC 
GCATCTCGGG GCGCAATTAG CGAAAGTGCG GCGCCCGCAA CGCGTCTCGA CCGACTGGCC 
TCGCGCTGTT TAAACACGGA CGTCAAGTTA GTTTTTTTGT TCACGGCCAA GGTGGTGCGC 
CTGGCCAGAG CGCACCTGCA CAAGACCAGC TCCTCGGCGG CTGGAGACCT GCGGTTCGAC 
TTGCTCACCC TGGTCGGGGA GAGCCTGCTG CCCTCCCTGT CCCTTCTCGA CTGCAACTGC 
TGCGTGGCCG ACGAGATCTG GTCCCTGCTC AAGCTCTACC CCTACCAGCA GAGGTACCGT 
CTGTACAGCC AGTGGAAGAA CGAGGCGTAC GTGAGTCACC CGTTGCTCAT TCGGGTGAAG 
GCGGACTCGC TCAAGAGGAT CAAGTACATC ATGAAGAGAC TCAGCAAGGA GAATGTGAAG 
CCTTCGGGGC GACAGATTGG CAAGCTCAGC CACAGCAACC CTTGCTTTCT CTTTGACTAT 
ATCCTGTCCC AGATCCAGAC CTGGGACAAC CTGATTGTGC CCGTGGTGGA CTCCCTCAAG 
TACCTCACCA TGCTGTCCTA CGACGTTCTC GCCTACTGCG TGGCGGAGGC GCTCTGCAAT 
CCGGAGAAGG ACCGCATGAA GCACGACGGC ACCAGCATCT CCCTCTGGCT GCAGAGTCTG 
GCCAGTTTCT GTGGCGCCGT CTTCAAGAAG TACTCCATCG ACCTGACGGG TCTGCTGCAG 
CTCGTGGCAA ATCAGCTGAA GGCAGAAAAA AGCCTTGACC TGCTGGTGCT GAAGGACATC 
GTACAGAAGA TGACCGGCAT CGAGTCGACG GAACAGGCAA CGCAGGACCA ACTGGAGGCG 
ATGTGCGGAG GCGAACTGCT TAAGGCCGAG GGTGGCTACT TCCACCAGCT GCGCAACACC 
AAGAAGTCGT CCCAGCGGCT GAAGGAGGCC CTGCTGGAGC AGGACCTGGC CCTGCCGCTC 
TGCCTGCTCA TGGCCCAGCA GAAGAACTGC ATCCTCTACC GGGACCAGGA GGCCTCCCAC 
CTCAAGCTGG TTGGAAAGCT CTACGACCAG TGCCAGGACA CCCTGGTCCA GTTTGGCAGC 
TTCCTGTCGA GCAGCCTGAG CACCGAAGAG TACGCCGGCC GCCTGCCGCC CGTGGACCAG 
CTGCTCTCGC GGTTCCACGT GCAGGCCGAC GTGGCCTTCT TTTTGGCCAG GCCCATGTTC 
GGACATTCCA TCGCGCTCAA GTTCGACGAC GCCAAGAGGA GGGACAAGAC GTTCAAAAGT 
CTAACCGCCG CGCAGAAGTT GCAGCGCTAC GTCGACGCGG TGGACCAGGT GATGTCGCGG 
GTCGTCGAGA GTGTGCGGCC GCTGCACCCG TCCAAGACGT GGGAGGACCT CAGCCCGCAG 
TTCTACGTCA CCTTCTGGTC GCTCTCGATG TACGACCTCC ACGTGCCCAC ATCCAGCTAC 
GAGAGGGAGG TGGGCAAGTT GAAGCAGCAG GTGGTGCAGG CGGAGGACAG CAAGGACATG 
GTGCCCAGCA AGCGCAAGAA GGAGCGTGAC CGGTGCGAAG CGCTCATGGA GAAGCTGCAG 
GAGGAGGAGC GGAAGCAGCA GGACCACTGC GGGCGCGTCC TGGCACGGCT GCGCAGCGAG 
AAGGACGCCT GGTTCCACTC CCGTTCGGCC AAGAACGAGA CCATCACGCA GTTCCTGCAG 
CTGTGCGTGT TCCCGCGCTG CACGTTCACG GCCCTGGACG CCCTCTACTG CGCCAAGTTT 
GTGCACCTGA TCCACATCCT GAAGACGCCA AACTTCTCCA CCCTCATCTG CTACGACAAG 
ATCTTTTGCG ACATCACTTA CACGGTGACG GCATGCACGG AGAACGAAGC GAACCGCTAC 
GGGCGCTTCC TCTGCTCCAT GCTGGAGACG GTGATGCGGT GGCACAGCGA CAAGGCCATC 
TTTGACAAGG AGTGTGCCAA CTTCCCCGGC TTCATGACCA AGTTCCGGGG CGCCAGCCAG 
TCGTCGGACA TTGCCACGGA CCACGTGGAC TACGAGAACT ACCGGCACGT CTGTCACAAG 
TGGCACTTCA AGATCACTAA GGCCCTGGTC ATCTGTCTGG AGTCTGGGGA CTACGTCCAG 
ATCCGGAACA GCCTGATTGT GCTCACCAAG GTGCTGCCCC ACTTCCCGGT CATGACCAAC 
TTGGGCCAGG CGCTGGAGCG GCGCATCGAG AAGATCCGCC AGGAGGAGAA GGAGAAGAGG 
CCGGACCTCT TTGCTCTTGC CACCGGCTAC GCCGGCCAGC TGAAGGGCAA GAAATCCGAG 
CTGATCCCAG AGCACGAGTT CCACCTCAAG GACAGCAAAG ACGGCCGCGC GGTGAGCGCC 
ACCGCACAGG CGAAGAGCGC CGCAGATCAC ACGGTGACGA CGGCGGCACG CGCCGCCACC 
TCTTCCCAAG TGGCGAGTGT TGATGGTGCC AAGGAGAAGC GGGGAGACTC CGTGAGGGAG 
TCCAACTCGT CACCAGCACT GTCCGGCAAT TCTTCCGGGA AGCTGTCGAG CAGCAGCGGA 
GGTCTCGTCC AGTCTTCGTC GTCGTCGTCG TCGTCGTCCA AGATGACATC GTCCAGTAGT 
TCTTCCGCCA CCCGCAGGGA GTCCAGCAGG TCGCTGAGCA AGGAGTCCGG GAGCGGGGAC 
GGACACGACA AGGGCGCCGC CACCGACCGC AAGGGCAGCT CGCTGTCCTC CCTCCACGAG 
CAGGACGTCA GGCCCTCCAA GGACGACATC CACAAAAATG ACCACTGGCT GGAGCTCGGG 
GACGGCAGTC CCCGGGGCCG TAGCCTCGGG GGCACGCAGA GCCCCAAGGT CTCGGAACCC 
GAGGGGAAGT CTGAGGACCG CGGGGGAGGG GGGGACCGCC CTTCTGAGAA GTGGGCTCAC 
CCCAAGGACT CCCACGACCC GGCCACGGGG GACAAGGGCA AGGCCAAGAA GGGCAGCCGC 
AAGCGGGACC ACTCCGACGA GCCCCCGCTC GAGGGCAAGC GCAAGAAGGA CGAGGACGCC 
TGTGAGTGCA GGGGGGACCA CGGGCCCCGG TTGGAGTGGG TTTGGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8029916 ( XM_029987813.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029843673.1 Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OIa29771
Clone ID Related Accession (Same CDS sequence)	XM_029987813.1
Accession Version	XM_029987813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4497bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	THO complex subunit 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021921043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGCC GGTCTGTACC GTTGGACATT TGTAAAAGTT GGGAAAGGAC GGGGAAATCG 
GACTTTGTCA AACTATGTCG CTCAGCGGCA TCATGCATGC AGACGTCGAG GCTGCCTTTC 
GACAAGGGTG GAAAAGAGAT GCAGCTTCTG TTACACGACC TCTGCTGGCA CGTGCTGGAG 
GATCGCCTGA AAGTTGAGCA AGCTGTGTCG GCGCTGGCCG AAGTGACTGC CTTGCATCAG 
GAGCTGCCCT CGATGCTGGC AGACGTCATC TTTCTGCTGG ACATGGAGAC GCTGTCGGTG 
GAGAACCGTG ACCAGAGGGA CCGTTTTTAC TGGCTCGTCG GCGGCTGTGC AAAGGTTGTT 
CCCGACTCGC TGCTGAAGGA GCGGCTGGAC ATCGAGACCT TGGGAGACGG CGGGATCGTG 
AAGAACAGCC GTGTCATGCT CACCAAGTTT GTCAAGATCA AGACACGACT GTTCTACAAG 
CAGAACAAGT ACAACCTGTT CCGGGAGGAG AGCGAGGGCT ACGCCAAGCT GACCACGGAG 
CTGAGCCAGG AGATCACCAG CAAGATCAGC CCCGATTACA TGATAGAAGT GATGCGCTCG 
CTCATTGGCT GCTTCAATCT GGACCCCAAC CGCGTGTTGG ACGTGGTTCT GGAGGCGTTT 
GAGTGCCGAC TGCATCTGGA GGACTTCTTT GTCCCTCTGC TGACAAAGTT CCTTCGCAAC 
CCTGCCACCA TAAGCCAGGT GCTGGGCTTC AAGTTCACCT TCTACCAGGT GAGGGGCGAA 
GCGGGAGAGC CCACCCCAGC GTCCCTGTAC CGGCTGGCCG CCCTCCTGAT CAGGAACGAG 
CTGATTGCCC TGGACGACCT CTACTCCCTG CTGCTGCCCG ACGACGGGGC CCTGTCGAGG 
CAGCACAAGA AGGAGGTGGC GGCGGCTAAG ACGTACGCCC GGAGAGCGAC CGTGGTGGTG 
GTCTCGTCGG ACAAGAAGGA CGAGGAGAAG AAGGAGGAGG ACAAGGACGA CGACCTGACC 
CTGGAGGAGA ACCAGAAGCT GGGCCTGTGT GAGGCCCTGC TGGAAGTGGG CGACTGGCCC 
AACGCCCAAA AGCTGATGGC GCGCCTGCCC GAGCACTACG CCGTGTCGCA GCCCCACATT 
GCCCGGCGCC TCTGCCTCCT GGTCCACACC CTGGTGGAGC CCTTGTACCG CAGGCACAGC 
GGGCTGCCGG AGGGCTTCCG GCGCTCGTAC GCGTCCTCCC GTCGGGGGGG CCTGCGGCCG 
CCCGTCCTCG TGGAGACCTT CGGGGACTTC CAGGCACGCG CCCTGCCCAT GCTCGTGGCC 
CTGGGCCCCA TGCTGCACCT GGACCCTGTG CTCATCGTCA AGGTGGTGCG CCTGGCCAGA 
GCGCACCTGC ACAAGACCAG CTCCTCGGCG GCTGGAGACC TGCGGTTCGA CTTGCTCACC 
CTGGTCGGGG AGAGCCTGCT GCCCTCCCTG TCCCTTCTCG ACTGCAACTG CTGCGTGGCC 
GACGAGATCT GGTCCCTGCT CAAGCTCTAC CCCTACCAGC AGAGGTACCG TCTGTACAGC 
CAGTGGAAGA ACGAGGCGTA CGTGAGTCAC CCGTTGCTCA TTCGGGTGAA GGCGGACTCG 
CTCAAGAGGA TCAAGTACAT CATGAAGAGA CTCAGCAAGG AAAATGTGAA GCCTTCGGGG 
CGACAGATTG GCAAGCTCAG CCACAGCAAC CCTTGCTTTC TCTTTGACTA TATCCTGTCC 
CAGATCCAGA CCTGGGACAA CCTGATCGTG CCCGTGGTGG ACTCCCTCAA GTACCTCACC 
ATGCTCTCCT ACGACGTTCT CGCCTACTGC GTGGCGGAGG CGCTCTGCAA TCCGGAGAAG 
GACCGCATGA AGCATGACGG CACCAGCATC TCCCTCTGGC TGCAGAGTCT GGCCAGTTTC 
TGTGGCGCCG TCTTCAAGAA GTACTCCATC GACCTGACGG GACTGCTGCA GCTCGTGGCA 
AATCAGCTGA AGGCAGAAAA AAGCCTTGAC CTGCTGGTGC TGAAGGACAT CGTACAGAAG 
ATGACCGGCA TCGAGTCGAC GGAACAGGCA ACGCAGGACC AACTGGAGGC GATGTGCGGA 
GGCGAACTGC TTAAGGCCGA GGGCGGCTAC TTCCACCAGC TGCGCAACAC CAAGAAGTCG 
TCCCAGCGGC TGAAGGAGGC CCTGCTGGAG CAGGACCTGG CCCTGCCGCT CTGCCTGCTC 
ATGGCCCAGC AGAAGAACTG CATCCTCTAC CGGGACCAGG AGGCCTCCCA CCTCAAGCTG 
GTTGGAAAGC TCTACGACCA GTGTCAGGAC ACCCTGGTCC AGTTTGGCAG CTTCCTGTCG 
AGCAGCCTGA GCACCGAAGA GTACGCCGGC CGCCTGCCGC CCGTGGACCA GCTACTCTCG 
CGGTTCCACG TGCAGGCCGA CGTGGCCTTC TTTTTGGCCA GGCCCATGTT CGGACATTCC 
ATCGCGCTCA AGTTCGACGA CGCCAAGAGG AGGGACAAGA CGTTCAAAAG TCTAACCGCC 
GCGCAGAAGT TGCAGCGCTA CGTCGACGCG GTGGACCAGG TGATGTCGCG GGTCGTCGAG 
AGTGTGCGGC CGCTGCACCC GTCCAAGACG TGGGAGGACC TCAGCCCGCA GTTCTACGTC 
ACCTTCTGGT CGCTCTCGAT GTACGACCTC CACGTGCCCA CGTCCAGCTA CGAGAGGGAG 
GTGGGCAAGT TGAAGCAGCA GGTGGTGCAG GCGGAGGACA GCAAGGACAT GGTGCCCAGC 
AAGCGCAAGA AGGAGCGCGA CCGGTGCGAA GCGCTCATGG AGAAGCTGCA GGAGGAGGAG 
CGCAAGCAGC AGGACCACTG CGGGCGCGTC CTGGCACGGC TGCGCAGCGA GAAGGATGCC 
TGGTTCCACT CCCGTTCGGC CAAGAACGAG ACCATCACGC AGTTCCTGCA GCTGTGCGTG 
TTCCCGCGCT GCACGTTCAC GGCCCTGGAC GCCCTCTACT GTGCCAAGTT TGTGCACCTG 
ATCCACATCC TGAAGACGCC AAACTTCTCC ACCCTCATCT GCTACGACAA GATCTTCTGC 
GACATCACTT ACACAGTGAC GGCATGCACG GAGAACGAAG CGAACCGCTA CGGGCGCTTC 
CTCTGCTCCA TGCTGGAGAC GGTGATGCGG TGGCACAGCG ACAAGGCCAT CTTTGACAAG 
GAGTGTGCCA ACTTCCCCGG CTTCATGACC AAGTTCCGGG GCGCCAGCCA GTCGTCGGAC 
ATTGCCACGG ACCACGTGGA CTACGAGAAC TACCGGCACG TCTGTCACAA GTGGCACTTC 
AAGATCACTA AGGCCCTGGT CATCTGTCTG GAGTCTGGGG ACTACGTCCA GATCCGGAAC 
AGCCTGATTG TGCTCACCAA GGTGCTGCCC CACTTCCCGG TCATGACCAA CTTGGGCCAG 
GCGCTGGAGC GGCGCATCGA GAAGATCCGC CAGGAGGAGA AGGAGAAGAG GCCGGACCTC 
TTTGCTCTTG CCACCGGCTA CGCCGGCCAG CTGAAGGGCA AGAAATCCGA GCTGATCCCG 
GAGCACGAGT TCCACCTCAA GGACAGCAAA GACGGCCGCG CGGTGAGCGC CACCGCACAG 
GTGAAGAGCG CTGCAGATCA CACGGTGACG ACGGCGGCAC ATGCCGCCAC CTCTTCCCAA 
GTGGCGAGTG TCGATGGTGC CAAGCAGAAG CGGGGAGACT CCGTGAGGGA GTCCAACTCG 
TCACCAGCAC TGTCCGGCAA TTCTTCTGGG AAGCTGTCGA GCAGCGGCGG AGGTCTCGTC 
CAGTCTTCGT CTTCGTCGTC GTCGTCCAAG ATGACATCGT CCAGTAGTTC TTCCGCCACC 
CGCAGGGAGT CCAACAGCTT TGGTGCGGTT GTGGAGAGTG CCACGACGGC CGCTTTTCCT 
CGGAGGTCGC TGAGCAAGGA GTCCGGGAGC GGGGACGGAC ACGACAAGGG CGCCGCCACC 
GACCGCAAGG GCAGCTCGCT GTCCTCCCTC CACGAGCAGG ACGTCAGGCC CTCCAAGGAC 
GACGTCCACA AAAATGACCA CTGGCTGGAG CCCGGGGACG CGCCCGGAGA CCACAAGCGG 
CGGCGCGTGG ACGGGGGCAG CAGCAGCGGC GGCAGCAGCA GTGGCAAGGA CTCCCACGAC 
CCGGCCACGG GGGACAAGGG CAAGGCCAAG AAGGGCAGCC GCAAGCGGGA CCACTCCGAC 
GAGCCCCCGC TCGAGGGCAA GCGCAAGAAG GACGAGGACG CCTTGAAGAG CTCGAGCAAG 
CGCAACGGGG AGGACTCTAA GGAGAAGGAG CGGTACCGGA GGGTGAGAGA GCAGCAGCCC 
CTGCTGCTGG GGCTCCCTCG TGACCAGCTG GTCGCACTCT CTCCTCAGGA AGAGGACAAG 
CTGCGAGAGT CCCCCAAGCT GCGAGAGCGC AGGGCCGACC GGGACGACAA GAAGCATCAT 
CGATCGAGCA GCAGCAGTGG CAGCTCGACG TCAACGAAAA AGCGATCATC CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029843673.1
CDS	13..4509
Translation	MAGRSVPLDICKSWERTGKSDFVKLCRSAASCMQTSRLPFDKGGKEMQLLLHDLCWHVLEDRLKVEQAVSALAEVTALHQELPSMLADVIFLLDMETLSVENRDQRDRFYWLVGGCAKVVPDSLLKERLDIETLGDGGIVKNSRVMLTKFVKIKTRLFYKQNKYNLFREESEGYAKLTTELSQEITSKISPDYMIEVMRSLIGCFNLDPNRVLDVVLEAFECRLHLEDFFVPLLTKFLRNPATISQVLGFKFTFYQVRGEAGEPTPASLYRLAALLIRNELIALDDLYSLLLPDDGALSRQHKKEVAAAKTYARRATVVVVSSDKKDEEKKEEDKDDDLTLEENQKLGLCEALLEVGDWPNAQKLMARLPEHYAVSQPHIARRLCLLVHTLVEPLYRRHSGLPEGFRRSYASSRRGGLRPPVLVETFGDFQARALPMLVALGPMLHLDPVLIVKVVRLARAHLHKTSSSAAGDLRFDLLTLVGESLLPSLSLLDCNCCVADEIWSLLKLYPYQQRYRLYSQWKNEAYVSHPLLIRVKADSLKRIKYIMKRLSKENVKPSGRQIGKLSHSNPCFLFDYILSQIQTWDNLIVPVVDSLKYLTMLSYDVLAYCVAEALCNPEKDRMKHDGTSISLWLQSLASFCGAVFKKYSIDLTGLLQLVANQLKAEKSLDLLVLKDIVQKMTGIESTEQATQDQLEAMCGGELLKAEGGYFHQLRNTKKSSQRLKEALLEQDLALPLCLLMAQQKNCILYRDQEASHLKLVGKLYDQCQDTLVQFGSFLSSSLSTEEYAGRLPPVDQLLSRFHVQADVAFFLARPMFGHSIALKFDDAKRRDKTFKSLTAAQKLQRYVDAVDQVMSRVVESVRPLHPSKTWEDLSPQFYVTFWSLSMYDLHVPTSSYEREVGKLKQQVVQAEDSKDMVPSKRKKERDRCEALMEKLQEEERKQQDHCGRVLARLRSEKDAWFHSRSAKNETITQFLQLCVFPRCTFTALDALYCAKFVHLIHILKTPNFSTLICYDKIFCDITYTVTACTENEANRYGRFLCSMLETVMRWHSDKAIFDKECANFPGFMTKFRGASQSSDIATDHVDYENYRHVCHKWHFKITKALVICLESGDYVQIRNSLIVLTKVLPHFPVMTNLGQALERRIEKIRQEEKEKRPDLFALATGYAGQLKGKKSELIPEHEFHLKDSKDGRAVSATAQVKSAADHTVTTAAHAATSSQVASVDGAKQKRGDSVRESNSSPALSGNSSGKLSSSGGGLVQSSSSSSSSKMTSSSSSSATRRESNSFGAVVESATTAAFPRRSLSKESGSGDGHDKGAATDRKGSSLSSLHEQDVRPSKDDVHKNDHWLEPGDAPGDHKRRRVDGGSSSGGSSSGKDSHDPATGDKGKAKKGSRKRDHSDEPPLEGKRKKDEDALKSSSKRNGEDSKEKERYRRVREQQPLLLGLPRDQLVALSPQEEDKLRESPKLRERRADRDDKKHHRSSSSSGSSTSTKKRSSF

Target ORF information:

RefSeq Version	XM_029987813.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029987813.1

ORF Insert Sequence:

ATGGCGGGCC GGTCTGTACC GTTGGACATT TGTAAAAGTT GGGAAAGGAC GGGGAAATCG 
GACTTTGTCA AACTATGTCG CTCAGCGGCA TCATGCATGC AGACGTCGAG GCTGCCTTTC 
GACAAGGGTG GAAAAGAGAT GCAGCTTCTG TTACACGACC TCTGCTGGCA CGTGCTGGAG 
GATCGCCTGA AAGTTGAGCA AGCTGTGTCG GCGCTGGCCG AAGTGACTGC CTTGCATCAG 
GAGCTGCCCT CGATGCTGGC AGACGTCATC TTTCTGCTGG ACATGGAGAC GCTGTCGGTG 
GAGAACCGTG ACCAGAGGGA CCGTTTTTAC TGGCTCGTCG GCGGCTGTGC AAAGGTTGTT 
CCCGACTCGC TGCTGAAGGA GCGGCTGGAC ATCGAGACCT TGGGAGACGG CGGGATCGTG 
AAGAACAGCC GTGTCATGCT CACCAAGTTT GTCAAGATCA AGACACGACT GTTCTACAAG 
CAGAACAAGT ACAACCTGTT CCGGGAGGAG AGCGAGGGCT ACGCCAAGCT GACCACGGAG 
CTGAGCCAGG AGATCACCAG CAAGATCAGC CCCGATTACA TGATAGAAGT GATGCGCTCG 
CTCATTGGCT GCTTCAATCT GGACCCCAAC CGCGTGTTGG ACGTGGTTCT GGAGGCGTTT 
GAGTGCCGAC TGCATCTGGA GGACTTCTTT GTCCCTCTGC TGACAAAGTT CCTTCGCAAC 
CCTGCCACCA TAAGCCAGGT GCTGGGCTTC AAGTTCACCT TCTACCAGGT GAGGGGCGAA 
GCGGGAGAGC CCACCCCAGC GTCCCTGTAC CGGCTGGCCG CCCTCCTGAT CAGGAACGAG 
CTGATTGCCC TGGACGACCT CTACTCCCTG CTGCTGCCCG ACGACGGGGC CCTGTCGAGG 
CAGCACAAGA AGGAGGTGGC GGCGGCTAAG ACGTACGCCC GGAGAGCGAC CGTGGTGGTG 
GTCTCGTCGG ACAAGAAGGA CGAGGAGAAG AAGGAGGAGG ACAAGGACGA CGACCTGACC 
CTGGAGGAGA ACCAGAAGCT GGGCCTGTGT GAGGCCCTGC TGGAAGTGGG CGACTGGCCC 
AACGCCCAAA AGCTGATGGC GCGCCTGCCC GAGCACTACG CCGTGTCGCA GCCCCACATT 
GCCCGGCGCC TCTGCCTCCT GGTCCACACC CTGGTGGAGC CCTTGTACCG CAGGCACAGC 
GGGCTGCCGG AGGGCTTCCG GCGCTCGTAC GCGTCCTCCC GTCGGGGGGG CCTGCGGCCG 
CCCGTCCTCG TGGAGACCTT CGGGGACTTC CAGGCACGCG CCCTGCCCAT GCTCGTGGCC 
CTGGGCCCCA TGCTGCACCT GGACCCTGTG CTCATCGTCA AGGTGGTGCG CCTGGCCAGA 
GCGCACCTGC ACAAGACCAG CTCCTCGGCG GCTGGAGACC TGCGGTTCGA CTTGCTCACC 
CTGGTCGGGG AGAGCCTGCT GCCCTCCCTG TCCCTTCTCG ACTGCAACTG CTGCGTGGCC 
GACGAGATCT GGTCCCTGCT CAAGCTCTAC CCCTACCAGC AGAGGTACCG TCTGTACAGC 
CAGTGGAAGA ACGAGGCGTA CGTGAGTCAC CCGTTGCTCA TTCGGGTGAA GGCGGACTCG 
CTCAAGAGGA TCAAGTACAT CATGAAGAGA CTCAGCAAGG AAAATGTGAA GCCTTCGGGG 
CGACAGATTG GCAAGCTCAG CCACAGCAAC CCTTGCTTTC TCTTTGACTA TATCCTGTCC 
CAGATCCAGA CCTGGGACAA CCTGATCGTG CCCGTGGTGG ACTCCCTCAA GTACCTCACC 
ATGCTCTCCT ACGACGTTCT CGCCTACTGC GTGGCGGAGG CGCTCTGCAA TCCGGAGAAG 
GACCGCATGA AGCATGACGG CACCAGCATC TCCCTCTGGC TGCAGAGTCT GGCCAGTTTC 
TGTGGCGCCG TCTTCAAGAA GTACTCCATC GACCTGACGG GACTGCTGCA GCTCGTGGCA 
AATCAGCTGA AGGCAGAAAA AAGCCTTGAC CTGCTGGTGC TGAAGGACAT CGTACAGAAG 
ATGACCGGCA TCGAGTCGAC GGAACAGGCA ACGCAGGACC AACTGGAGGC GATGTGCGGA 
GGCGAACTGC TTAAGGCCGA GGGCGGCTAC TTCCACCAGC TGCGCAACAC CAAGAAGTCG 
TCCCAGCGGC TGAAGGAGGC CCTGCTGGAG CAGGACCTGG CCCTGCCGCT CTGCCTGCTC 
ATGGCCCAGC AGAAGAACTG CATCCTCTAC CGGGACCAGG AGGCCTCCCA CCTCAAGCTG 
GTTGGAAAGC TCTACGACCA GTGTCAGGAC ACCCTGGTCC AGTTTGGCAG CTTCCTGTCG 
AGCAGCCTGA GCACCGAAGA GTACGCCGGC CGCCTGCCGC CCGTGGACCA GCTACTCTCG 
CGGTTCCACG TGCAGGCCGA CGTGGCCTTC TTTTTGGCCA GGCCCATGTT CGGACATTCC 
ATCGCGCTCA AGTTCGACGA CGCCAAGAGG AGGGACAAGA CGTTCAAAAG TCTAACCGCC 
GCGCAGAAGT TGCAGCGCTA CGTCGACGCG GTGGACCAGG TGATGTCGCG GGTCGTCGAG 
AGTGTGCGGC CGCTGCACCC GTCCAAGACG TGGGAGGACC TCAGCCCGCA GTTCTACGTC 
ACCTTCTGGT CGCTCTCGAT GTACGACCTC CACGTGCCCA CGTCCAGCTA CGAGAGGGAG 
GTGGGCAAGT TGAAGCAGCA GGTGGTGCAG GCGGAGGACA GCAAGGACAT GGTGCCCAGC 
AAGCGCAAGA AGGAGCGCGA CCGGTGCGAA GCGCTCATGG AGAAGCTGCA GGAGGAGGAG 
CGCAAGCAGC AGGACCACTG CGGGCGCGTC CTGGCACGGC TGCGCAGCGA GAAGGATGCC 
TGGTTCCACT CCCGTTCGGC CAAGAACGAG ACCATCACGC AGTTCCTGCA GCTGTGCGTG 
TTCCCGCGCT GCACGTTCAC GGCCCTGGAC GCCCTCTACT GTGCCAAGTT TGTGCACCTG 
ATCCACATCC TGAAGACGCC AAACTTCTCC ACCCTCATCT GCTACGACAA GATCTTCTGC 
GACATCACTT ACACAGTGAC GGCATGCACG GAGAACGAAG CGAACCGCTA CGGGCGCTTC 
CTCTGCTCCA TGCTGGAGAC GGTGATGCGG TGGCACAGCG ACAAGGCCAT CTTTGACAAG 
GAGTGTGCCA ACTTCCCCGG CTTCATGACC AAGTTCCGGG GCGCCAGCCA GTCGTCGGAC 
ATTGCCACGG ACCACGTGGA CTACGAGAAC TACCGGCACG TCTGTCACAA GTGGCACTTC 
AAGATCACTA AGGCCCTGGT CATCTGTCTG GAGTCTGGGG ACTACGTCCA GATCCGGAAC 
AGCCTGATTG TGCTCACCAA GGTGCTGCCC CACTTCCCGG TCATGACCAA CTTGGGCCAG 
GCGCTGGAGC GGCGCATCGA GAAGATCCGC CAGGAGGAGA AGGAGAAGAG GCCGGACCTC 
TTTGCTCTTG CCACCGGCTA CGCCGGCCAG CTGAAGGGCA AGAAATCCGA GCTGATCCCG 
GAGCACGAGT TCCACCTCAA GGACAGCAAA GACGGCCGCG CGGTGAGCGC CACCGCACAG 
GTGAAGAGCG CTGCAGATCA CACGGTGACG ACGGCGGCAC ATGCCGCCAC CTCTTCCCAA 
GTGGCGAGTG TCGATGGTGC CAAGCAGAAG CGGGGAGACT CCGTGAGGGA GTCCAACTCG 
TCACCAGCAC TGTCCGGCAA TTCTTCTGGG AAGCTGTCGA GCAGCGGCGG AGGTCTCGTC 
CAGTCTTCGT CTTCGTCGTC GTCGTCCAAG ATGACATCGT CCAGTAGTTC TTCCGCCACC 
CGCAGGGAGT CCAACAGCTT TGGTGCGGTT GTGGAGAGTG CCACGACGGC CGCTTTTCCT 
CGGAGGTCGC TGAGCAAGGA GTCCGGGAGC GGGGACGGAC ACGACAAGGG CGCCGCCACC 
GACCGCAAGG GCAGCTCGCT GTCCTCCCTC CACGAGCAGG ACGTCAGGCC CTCCAAGGAC 
GACGTCCACA AAAATGACCA CTGGCTGGAG CCCGGGGACG CGCCCGGAGA CCACAAGCGG 
CGGCGCGTGG ACGGGGGCAG CAGCAGCGGC GGCAGCAGCA GTGGCAAGGA CTCCCACGAC 
CCGGCCACGG GGGACAAGGG CAAGGCCAAG AAGGGCAGCC GCAAGCGGGA CCACTCCGAC 
GAGCCCCCGC TCGAGGGCAA GCGCAAGAAG GACGAGGACG CCTTGAAGAG CTCGAGCAAG 
CGCAACGGGG AGGACTCTAA GGAGAAGGAG CGGTACCGGA GGGTGAGAGA GCAGCAGCCC 
CTGCTGCTGG GGCTCCCTCG TGACCAGCTG GTCGCACTCT CTCCTCAGGA AGAGGACAAG 
CTGCGAGAGT CCCCCAAGCT GCGAGAGCGC AGGGCCGACC GGGACGACAA GAAGCATCAT 
CGATCGAGCA GCAGCAGTGG CAGCTCGACG TCAACGAAAA AGCGATCATC CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8029916 ( XM_029987808.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029843668.1 Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X1, mRNA.

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CloneID	OIa29766
Clone ID Related Accession (Same CDS sequence)	XM_029987808.1
Accession Version	XM_029987808.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	THO complex subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021921043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGCC GGTCTGTACC GTTGGACATT TGTAAAAGTT GGGAAAGGAC GGGGAAATCG 
GACTTTGTCA AACTATGTCG CTCAGCGGCA TCATGCATGC AGACGTCGAG GCTGCCTTTC 
GACAAGGGTG GAAAAGAGAT GCAGCTTCTG TTACACGACC TCTGCTGGCA CGTGCTGGAG 
GATCGCCTGA AAGTTGAGCA AGCTGTGTCG GCGCTGGCCG AAGTGACTGC CTTGCATCAG 
GAGCTGCCCT CGATGCTGGC AGACGTCATC TTTCTGCTGG ACATGGAGAC GCTGTCGGTG 
GAGAACCGTG ACCAGAGGGA CCGTTTTTAC TGGCTCGTCG GCGGCTGTGC AAAGGTTGTT 
CCCGACTCGC TGCTGAAGGA GCGGCTGGAC ATCGAGACCT TGGGAGACGG CGGGATCGTG 
AAGAACAGCC GTGTCATGCT CACCAAGTTT GTCAAGATCA AGACACGACT GTTCTACAAG 
CAGAACAAGT ACAACCTGTT CCGGGAGGAG AGCGAGGGCT ACGCCAAGCT GACCACGGAG 
CTGAGCCAGG AGATCACCAG CAAGATCAGC CCCGATTACA TGATAGAAGT GATGCGCTCG 
CTCATTGGCT GCTTCAATCT GGACCCCAAC CGCGTGTTGG ACGTGGTTCT GGAGGCGTTT 
GAGTGCCGAC TGCATCTGGA GGACTTCTTT GTCCCTCTGC TGACAAAGTT CCTTCGCAAC 
CCTGCCACCA TAAGCCAGGT GCTGGGCTTC AAGTTCACCT TCTACCAGGT GAGGGGCGAA 
GCGGGAGAGC CCACCCCAGC GTCCCTGTAC CGGCTGGCCG CCCTCCTGAT CAGGAACGAG 
CTGATTGCCC TGGACGACCT CTACTCCCTG CTGCTGCCCG ACGACGGGGC CCTGTCGAGG 
CAGCACAAGA AGGAGGTGGC GGCGGCTAAG ACGTACGCCC GGAGAGCGAC CGTGGTGGTG 
GTCTCGTCGG ACAAGAAGGA CGAGGAGAAG AAGGAGGAGG ACAAGGACGA CGACCTGACC 
CTGGAGGAGA ACCAGAAGCT GGGCCTGTGT GAGGCCCTGC TGGAAGTGGG CGACTGGCCC 
AACGCCCAAA AGCTGATGGC GCGCCTGCCC GAGCACTACG CCGTGTCGCA GCCCCACATT 
GCCCGGCGCC TCTGCCTCCT GGTCCACACC CTGGTGGAGC CCTTGTACCG CAGGCACAGC 
GGGCTGCCGG AGGGCTTCCG GCGCTCGTAC GCGTCCTCCC GTCGGGGGGG CCTGCGGCCG 
CCCGTCCTCG TGGAGACCTT CGGGGACTTC CAGGCACGCG CCCTGCCCAT GCTCGTGGCC 
CTGGGCCCCA TGCTGCACCT GGACCCTGTG CTCATCGTCA AGGTGGTGCG CCTGGCCAGA 
GCGCACCTGC ACAAGACCAG CTCCTCGGCG GCTGGAGACC TGCGGTTCGA CTTGCTCACC 
CTGGTCGGGG AGAGCCTGCT GCCCTCCCTG TCCCTTCTCG ACTGCAACTG CTGCGTGGCC 
GACGAGATCT GGTCCCTGCT CAAGCTCTAC CCCTACCAGC AGAGGTACCG TCTGTACAGC 
CAGTGGAAGA ACGAGGCGTA CGTGAGTCAC CCGTTGCTCA TTCGGGTGAA GGCGGACTCG 
CTCAAGAGGA TCAAGTACAT CATGAAGAGA CTCAGCAAGG AAAATGTGAA GCCTTCGGGG 
CGACAGATTG GCAAGCTCAG CCACAGCAAC CCTTGCTTTC TCTTTGACTA TATCCTGTCC 
CAGATCCAGA CCTGGGACAA CCTGATCGTG CCCGTGGTGG ACTCCCTCAA GTACCTCACC 
ATGCTCTCCT ACGACGTTCT CGCCTACTGC GTGGCGGAGG CGCTCTGCAA TCCGGAGAAG 
GACCGCATGA AGCATGACGG CACCAGCATC TCCCTCTGGC TGCAGAGTCT GGCCAGTTTC 
TGTGGCGCCG TCTTCAAGAA GTACTCCATC GACCTGACGG GACTGCTGCA GCTCGTGGCA 
AATCAGCTGA AGGCAGAAAA AAGCCTTGAC CTGCTGGTGC TGAAGGACAT CGTACAGAAG 
ATGACCGGCA TCGAGTCGAC GGAACAGGCA ACGCAGGACC AACTGGAGGC GATGTGCGGA 
GGCGAACTGC TTAAGGCCGA GGGCGGCTAC TTCCACCAGC TGCGCAACAC CAAGAAGTCG 
TCCCAGCGGC TGAAGGAGGC CCTGCTGGAG CAGGACCTGG CCCTGCCGCT CTGCCTGCTC 
ATGGCCCAGC AGAAGAACTG CATCCTCTAC CGGGACCAGG AGGCCTCCCA CCTCAAGCTG 
GTTGGAAAGC TCTACGACCA GTGTCAGGAC ACCCTGGTCC AGTTTGGCAG CTTCCTGTCG 
AGCAGCCTGA GCACCGAAGA GTACGCCGGC CGCCTGCCGC CCGTGGACCA GCTACTCTCG 
CGGTTCCACG TGCAGGCCGA CGTGGCCTTC TTTTTGGCCA GGCCCATGTT CGGACATTCC 
ATCGCGCTCA AGTTCGACGA CGCCAAGAGG AGGGACAAGA CGTTCAAAAG TCTAACCGCC 
GCGCAGAAGT TGCAGCGCTA CGTCGACGCG GTGGACCAGG TGATGTCGCG GGTCGTCGAG 
AGTGTGCGGC CGCTGCACCC GTCCAAGACG TGGGAGGACC TCAGCCCGCA GTTCTACGTC 
ACCTTCTGGT CGCTCTCGAT GTACGACCTC CACGTGCCCA CGTCCAGCTA CGAGAGGGAG 
GTGGGCAAGT TGAAGCAGCA GGTGGTGCAG GCGGAGGACA GCAAGGACAT GGTGCCCAGC 
AAGCGCAAGA AGGAGCGCGA CCGGTGCGAA GCGCTCATGG AGAAGCTGCA GGAGGAGGAG 
CGCAAGCAGC AGGACCACTG CGGGCGCGTC CTGGCACGGC TGCGCAGCGA GAAGGATGCC 
TGGTTCCACT CCCGTTCGGC CAAGAACGAG ACCATCACGC AGTTCCTGCA GCTGTGCGTG 
TTCCCGCGCT GCACGTTCAC GGCCCTGGAC GCCCTCTACT GTGCCAAGTT TGTGCACCTG 
ATCCACATCC TGAAGACGCC AAACTTCTCC ACCCTCATCT GCTACGACAA GATCTTCTGC 
GACATCACTT ACACAGTGAC GGCATGCACG GAGAACGAAG CGAACCGCTA CGGGCGCTTC 
CTCTGCTCCA TGCTGGAGAC GGTGATGCGG TGGCACAGCG ACAAGGCCAT CTTTGACAAG 
GAGTGTGCCA ACTTCCCCGG CTTCATGACC AAGTTCCGGG GCGCCAGCCA GTCGTCGGAC 
ATTGCCACGG ACCACGTGGA CTACGAGAAC TACCGGCACG TCTGTCACAA GTGGCACTTC 
AAGATCACTA AGGCCCTGGT CATCTGTCTG GAGTCTGGGG ACTACGTCCA GATCCGGAAC 
AGCCTGATTG TGCTCACCAA GGTGCTGCCC CACTTCCCGG TCATGACCAA CTTGGGCCAG 
GCGCTGGAGC GGCGCATCGA GAAGATCCGC CAGGAGGAGA AGGAGAAGAG GCCGGACCTC 
TTTGCTCTTG CCACCGGCTA CGCCGGCCAG CTGAAGGGCA AGAAATCCGA GCTGATCCCG 
GAGCACGAGT TCCACCTCAA GGACAGCAAA GACGGCCGCG CGGTGAGCGC CACCGCACAG 
GTGAAGAGCG CTGCAGATCA CACGGTGACG ACGGCGGCAC ATGCCGCCAC CTCTTCCCAA 
GTGGCGAGTG TCGATGGTGC CAAGCAGAAG CGGGGAGACT CCGTGAGGGA GTCCAACTCG 
TCACCAGCAC TGTCCGGCAA TTCTTCTGGG AAGCTGTCGA GCAGCGGCGG AGGTCTCGTC 
CAGTCTTCGT CTTCGTCGTC GTCGTCCAAG ATGACATCGT CCAGTAGTTC TTCCGCCACC 
CGCAGGGAGT CCAACAGCTT TGGTGCGGTT GTGGAGAGTG CCACGACGGC CGCTTTTCCT 
CGGAGGTCGC TGAGCAAGGA GTCCGGGAGC GGGGACGGAC ACGACAAGGG CGCCGCCACC 
GACCGCAAGG GCAGCTCGCT GTCCTCCCTC CACGAGCAGG ACGTCAGGCC CTCCAAGGAC 
GACGTCCACA AAAATGACCA CTGGCTGGAG CCCGGGGACG CGCCCGGAGA CCACAAGCGG 
CGGCGCGTGG ACGGGGGCAG CAGCAGCGGC GGCAGCAGCA GTGGCAAGGT GGGAGGCAGT 
CCCCGGGGCC GTAGCCTCGG GGGCACGCAG AGCCCCAAGG TCTCGGAACC CGAGGGGAAG 
TCTGAGGACC GCGGGGGAGG GGGGGACCGC CCTTCTGAGA AGTGGGCTCA CCCCAAGGAC 
TCCCACGACC CGGCCACGGG GGACAAGGGC AAGGCCAAGA AGGGCAGCCG CAAGCGGGAC 
CACTCCGACG AGCCCCCGCT CGAGGGCAAG CGCAAGAAGG ACGAGGACGC CTTGAAGAGC 
TCGAGCAAGC GCAACGGGGA GGACTCTAAG GAGAAGGAGC GGTACCGGAG GGTGAGAGAG 
CAGCAGCCCC TGCTGCTGGG GCTCCCTCGT GACCAGCTGG TCGCACTCTC TCCTCAGGAA 
GAGGACAAGC TGCGAGAGTC CCCCAAGCTG CGAGAGCGCA GGGCCGACCG GGACGACAAG 
AAGCATCATC GATCGAGCAG CAGCAGTGGC AGCTCGACGT CAACGAAAAA GCGATCATCC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029843668.1
CDS	13..4638
Translation	MAGRSVPLDICKSWERTGKSDFVKLCRSAASCMQTSRLPFDKGGKEMQLLLHDLCWHVLEDRLKVEQAVSALAEVTALHQELPSMLADVIFLLDMETLSVENRDQRDRFYWLVGGCAKVVPDSLLKERLDIETLGDGGIVKNSRVMLTKFVKIKTRLFYKQNKYNLFREESEGYAKLTTELSQEITSKISPDYMIEVMRSLIGCFNLDPNRVLDVVLEAFECRLHLEDFFVPLLTKFLRNPATISQVLGFKFTFYQVRGEAGEPTPASLYRLAALLIRNELIALDDLYSLLLPDDGALSRQHKKEVAAAKTYARRATVVVVSSDKKDEEKKEEDKDDDLTLEENQKLGLCEALLEVGDWPNAQKLMARLPEHYAVSQPHIARRLCLLVHTLVEPLYRRHSGLPEGFRRSYASSRRGGLRPPVLVETFGDFQARALPMLVALGPMLHLDPVLIVKVVRLARAHLHKTSSSAAGDLRFDLLTLVGESLLPSLSLLDCNCCVADEIWSLLKLYPYQQRYRLYSQWKNEAYVSHPLLIRVKADSLKRIKYIMKRLSKENVKPSGRQIGKLSHSNPCFLFDYILSQIQTWDNLIVPVVDSLKYLTMLSYDVLAYCVAEALCNPEKDRMKHDGTSISLWLQSLASFCGAVFKKYSIDLTGLLQLVANQLKAEKSLDLLVLKDIVQKMTGIESTEQATQDQLEAMCGGELLKAEGGYFHQLRNTKKSSQRLKEALLEQDLALPLCLLMAQQKNCILYRDQEASHLKLVGKLYDQCQDTLVQFGSFLSSSLSTEEYAGRLPPVDQLLSRFHVQADVAFFLARPMFGHSIALKFDDAKRRDKTFKSLTAAQKLQRYVDAVDQVMSRVVESVRPLHPSKTWEDLSPQFYVTFWSLSMYDLHVPTSSYEREVGKLKQQVVQAEDSKDMVPSKRKKERDRCEALMEKLQEEERKQQDHCGRVLARLRSEKDAWFHSRSAKNETITQFLQLCVFPRCTFTALDALYCAKFVHLIHILKTPNFSTLICYDKIFCDITYTVTACTENEANRYGRFLCSMLETVMRWHSDKAIFDKECANFPGFMTKFRGASQSSDIATDHVDYENYRHVCHKWHFKITKALVICLESGDYVQIRNSLIVLTKVLPHFPVMTNLGQALERRIEKIRQEEKEKRPDLFALATGYAGQLKGKKSELIPEHEFHLKDSKDGRAVSATAQVKSAADHTVTTAAHAATSSQVASVDGAKQKRGDSVRESNSSPALSGNSSGKLSSSGGGLVQSSSSSSSSKMTSSSSSSATRRESNSFGAVVESATTAAFPRRSLSKESGSGDGHDKGAATDRKGSSLSSLHEQDVRPSKDDVHKNDHWLEPGDAPGDHKRRRVDGGSSSGGSSSGKVGGSPRGRSLGGTQSPKVSEPEGKSEDRGGGGDRPSEKWAHPKDSHDPATGDKGKAKKGSRKRDHSDEPPLEGKRKKDEDALKSSSKRNGEDSKEKERYRRVREQQPLLLGLPRDQLVALSPQEEDKLRESPKLRERRADRDDKKHHRSSSSSGSSTSTKKRSSF

Target ORF information:

RefSeq Version	XM_029987808.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029987808.1

ORF Insert Sequence:

ATGGCGGGCC GGTCTGTACC GTTGGACATT TGTAAAAGTT GGGAAAGGAC GGGGAAATCG 
GACTTTGTCA AACTATGTCG CTCAGCGGCA TCATGCATGC AGACGTCGAG GCTGCCTTTC 
GACAAGGGTG GAAAAGAGAT GCAGCTTCTG TTACACGACC TCTGCTGGCA CGTGCTGGAG 
GATCGCCTGA AAGTTGAGCA AGCTGTGTCG GCGCTGGCCG AAGTGACTGC CTTGCATCAG 
GAGCTGCCCT CGATGCTGGC AGACGTCATC TTTCTGCTGG ACATGGAGAC GCTGTCGGTG 
GAGAACCGTG ACCAGAGGGA CCGTTTTTAC TGGCTCGTCG GCGGCTGTGC AAAGGTTGTT 
CCCGACTCGC TGCTGAAGGA GCGGCTGGAC ATCGAGACCT TGGGAGACGG CGGGATCGTG 
AAGAACAGCC GTGTCATGCT CACCAAGTTT GTCAAGATCA AGACACGACT GTTCTACAAG 
CAGAACAAGT ACAACCTGTT CCGGGAGGAG AGCGAGGGCT ACGCCAAGCT GACCACGGAG 
CTGAGCCAGG AGATCACCAG CAAGATCAGC CCCGATTACA TGATAGAAGT GATGCGCTCG 
CTCATTGGCT GCTTCAATCT GGACCCCAAC CGCGTGTTGG ACGTGGTTCT GGAGGCGTTT 
GAGTGCCGAC TGCATCTGGA GGACTTCTTT GTCCCTCTGC TGACAAAGTT CCTTCGCAAC 
CCTGCCACCA TAAGCCAGGT GCTGGGCTTC AAGTTCACCT TCTACCAGGT GAGGGGCGAA 
GCGGGAGAGC CCACCCCAGC GTCCCTGTAC CGGCTGGCCG CCCTCCTGAT CAGGAACGAG 
CTGATTGCCC TGGACGACCT CTACTCCCTG CTGCTGCCCG ACGACGGGGC CCTGTCGAGG 
CAGCACAAGA AGGAGGTGGC GGCGGCTAAG ACGTACGCCC GGAGAGCGAC CGTGGTGGTG 
GTCTCGTCGG ACAAGAAGGA CGAGGAGAAG AAGGAGGAGG ACAAGGACGA CGACCTGACC 
CTGGAGGAGA ACCAGAAGCT GGGCCTGTGT GAGGCCCTGC TGGAAGTGGG CGACTGGCCC 
AACGCCCAAA AGCTGATGGC GCGCCTGCCC GAGCACTACG CCGTGTCGCA GCCCCACATT 
GCCCGGCGCC TCTGCCTCCT GGTCCACACC CTGGTGGAGC CCTTGTACCG CAGGCACAGC 
GGGCTGCCGG AGGGCTTCCG GCGCTCGTAC GCGTCCTCCC GTCGGGGGGG CCTGCGGCCG 
CCCGTCCTCG TGGAGACCTT CGGGGACTTC CAGGCACGCG CCCTGCCCAT GCTCGTGGCC 
CTGGGCCCCA TGCTGCACCT GGACCCTGTG CTCATCGTCA AGGTGGTGCG CCTGGCCAGA 
GCGCACCTGC ACAAGACCAG CTCCTCGGCG GCTGGAGACC TGCGGTTCGA CTTGCTCACC 
CTGGTCGGGG AGAGCCTGCT GCCCTCCCTG TCCCTTCTCG ACTGCAACTG CTGCGTGGCC 
GACGAGATCT GGTCCCTGCT CAAGCTCTAC CCCTACCAGC AGAGGTACCG TCTGTACAGC 
CAGTGGAAGA ACGAGGCGTA CGTGAGTCAC CCGTTGCTCA TTCGGGTGAA GGCGGACTCG 
CTCAAGAGGA TCAAGTACAT CATGAAGAGA CTCAGCAAGG AAAATGTGAA GCCTTCGGGG 
CGACAGATTG GCAAGCTCAG CCACAGCAAC CCTTGCTTTC TCTTTGACTA TATCCTGTCC 
CAGATCCAGA CCTGGGACAA CCTGATCGTG CCCGTGGTGG ACTCCCTCAA GTACCTCACC 
ATGCTCTCCT ACGACGTTCT CGCCTACTGC GTGGCGGAGG CGCTCTGCAA TCCGGAGAAG 
GACCGCATGA AGCATGACGG CACCAGCATC TCCCTCTGGC TGCAGAGTCT GGCCAGTTTC 
TGTGGCGCCG TCTTCAAGAA GTACTCCATC GACCTGACGG GACTGCTGCA GCTCGTGGCA 
AATCAGCTGA AGGCAGAAAA AAGCCTTGAC CTGCTGGTGC TGAAGGACAT CGTACAGAAG 
ATGACCGGCA TCGAGTCGAC GGAACAGGCA ACGCAGGACC AACTGGAGGC GATGTGCGGA 
GGCGAACTGC TTAAGGCCGA GGGCGGCTAC TTCCACCAGC TGCGCAACAC CAAGAAGTCG 
TCCCAGCGGC TGAAGGAGGC CCTGCTGGAG CAGGACCTGG CCCTGCCGCT CTGCCTGCTC 
ATGGCCCAGC AGAAGAACTG CATCCTCTAC CGGGACCAGG AGGCCTCCCA CCTCAAGCTG 
GTTGGAAAGC TCTACGACCA GTGTCAGGAC ACCCTGGTCC AGTTTGGCAG CTTCCTGTCG 
AGCAGCCTGA GCACCGAAGA GTACGCCGGC CGCCTGCCGC CCGTGGACCA GCTACTCTCG 
CGGTTCCACG TGCAGGCCGA CGTGGCCTTC TTTTTGGCCA GGCCCATGTT CGGACATTCC 
ATCGCGCTCA AGTTCGACGA CGCCAAGAGG AGGGACAAGA CGTTCAAAAG TCTAACCGCC 
GCGCAGAAGT TGCAGCGCTA CGTCGACGCG GTGGACCAGG TGATGTCGCG GGTCGTCGAG 
AGTGTGCGGC CGCTGCACCC GTCCAAGACG TGGGAGGACC TCAGCCCGCA GTTCTACGTC 
ACCTTCTGGT CGCTCTCGAT GTACGACCTC CACGTGCCCA CGTCCAGCTA CGAGAGGGAG 
GTGGGCAAGT TGAAGCAGCA GGTGGTGCAG GCGGAGGACA GCAAGGACAT GGTGCCCAGC 
AAGCGCAAGA AGGAGCGCGA CCGGTGCGAA GCGCTCATGG AGAAGCTGCA GGAGGAGGAG 
CGCAAGCAGC AGGACCACTG CGGGCGCGTC CTGGCACGGC TGCGCAGCGA GAAGGATGCC 
TGGTTCCACT CCCGTTCGGC CAAGAACGAG ACCATCACGC AGTTCCTGCA GCTGTGCGTG 
TTCCCGCGCT GCACGTTCAC GGCCCTGGAC GCCCTCTACT GTGCCAAGTT TGTGCACCTG 
ATCCACATCC TGAAGACGCC AAACTTCTCC ACCCTCATCT GCTACGACAA GATCTTCTGC 
GACATCACTT ACACAGTGAC GGCATGCACG GAGAACGAAG CGAACCGCTA CGGGCGCTTC 
CTCTGCTCCA TGCTGGAGAC GGTGATGCGG TGGCACAGCG ACAAGGCCAT CTTTGACAAG 
GAGTGTGCCA ACTTCCCCGG CTTCATGACC AAGTTCCGGG GCGCCAGCCA GTCGTCGGAC 
ATTGCCACGG ACCACGTGGA CTACGAGAAC TACCGGCACG TCTGTCACAA GTGGCACTTC 
AAGATCACTA AGGCCCTGGT CATCTGTCTG GAGTCTGGGG ACTACGTCCA GATCCGGAAC 
AGCCTGATTG TGCTCACCAA GGTGCTGCCC CACTTCCCGG TCATGACCAA CTTGGGCCAG 
GCGCTGGAGC GGCGCATCGA GAAGATCCGC CAGGAGGAGA AGGAGAAGAG GCCGGACCTC 
TTTGCTCTTG CCACCGGCTA CGCCGGCCAG CTGAAGGGCA AGAAATCCGA GCTGATCCCG 
GAGCACGAGT TCCACCTCAA GGACAGCAAA GACGGCCGCG CGGTGAGCGC CACCGCACAG 
GTGAAGAGCG CTGCAGATCA CACGGTGACG ACGGCGGCAC ATGCCGCCAC CTCTTCCCAA 
GTGGCGAGTG TCGATGGTGC CAAGCAGAAG CGGGGAGACT CCGTGAGGGA GTCCAACTCG 
TCACCAGCAC TGTCCGGCAA TTCTTCTGGG AAGCTGTCGA GCAGCGGCGG AGGTCTCGTC 
CAGTCTTCGT CTTCGTCGTC GTCGTCCAAG ATGACATCGT CCAGTAGTTC TTCCGCCACC 
CGCAGGGAGT CCAACAGCTT TGGTGCGGTT GTGGAGAGTG CCACGACGGC CGCTTTTCCT 
CGGAGGTCGC TGAGCAAGGA GTCCGGGAGC GGGGACGGAC ACGACAAGGG CGCCGCCACC 
GACCGCAAGG GCAGCTCGCT GTCCTCCCTC CACGAGCAGG ACGTCAGGCC CTCCAAGGAC 
GACGTCCACA AAAATGACCA CTGGCTGGAG CCCGGGGACG CGCCCGGAGA CCACAAGCGG 
CGGCGCGTGG ACGGGGGCAG CAGCAGCGGC GGCAGCAGCA GTGGCAAGGT GGGAGGCAGT 
CCCCGGGGCC GTAGCCTCGG GGGCACGCAG AGCCCCAAGG TCTCGGAACC CGAGGGGAAG 
TCTGAGGACC GCGGGGGAGG GGGGGACCGC CCTTCTGAGA AGTGGGCTCA CCCCAAGGAC 
TCCCACGACC CGGCCACGGG GGACAAGGGC AAGGCCAAGA AGGGCAGCCG CAAGCGGGAC 
CACTCCGACG AGCCCCCGCT CGAGGGCAAG CGCAAGAAGG ACGAGGACGC CTTGAAGAGC 
TCGAGCAAGC GCAACGGGGA GGACTCTAAG GAGAAGGAGC GGTACCGGAG GGTGAGAGAG 
CAGCAGCCCC TGCTGCTGGG GCTCCCTCGT GACCAGCTGG TCGCACTCTC TCCTCAGGAA 
GAGGACAAGC TGCGAGAGTC CCCCAAGCTG CGAGAGCGCA GGGCCGACCG GGACGACAAG 
AAGCATCATC GATCGAGCAG CAGCAGTGGC AGCTCGACGT CAACGAAAAA GCGATCATCC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8029916 ( XM_029987809.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029843669.1 Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X2, mRNA.

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CloneID	OIa29767
Clone ID Related Accession (Same CDS sequence)	XM_029987809.1
Accession Version	XM_029987809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	THO complex subunit 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021921043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGCC GGTCTGTACC GTTGGACATT TGTAAAAGTT GGGAAAGGAC GGGGAAATCG 
GACTTTGTCA AACTATGTCG CTCAGCGGCA TCATGCATGC AGACGTCGAG GCTGCCTTTC 
GACAAGGGTG GAAAAGAGAT GCAGCTTCTG TTACACGACC TCTGCTGGCA CGTGCTGGAG 
GATCGCCTGA AAGTTGAGCA AGCTGTGTCG GCGCTGGCCG AAGTGACTGC CTTGCATCAG 
GAGCTGCCCT CGATGCTGGC AGACGTCATC TTTCTGCTGG ACATGGAGAC GCTGTCGGTG 
GAGAACCGTG ACCAGAGGGA CCGTTTTTAC TGGCTCGTCG GCGGCTGTGC AAAGGTTGTT 
CCCGACTCGC TGCTGAAGGA GCGGCTGGAC ATCGAGACCT TGGGAGACGG CGGGATCGTG 
AAGAACAGCC GTGTCATGCT CACCAAGTTT GTCAAGATCA AGACACGACT GTTCTACAAG 
CAGAACAAGT ACAACCTGTT CCGGGAGGAG AGCGAGGGCT ACGCCAAGCT GACCACGGAG 
CTGAGCCAGG AGATCACCAG CAAGATCAGC CCCGATTACA TGATAGAAGT GATGCGCTCG 
CTCATTGGCT GCTTCAATCT GGACCCCAAC CGCGTGTTGG ACGTGGTTCT GGAGGCGTTT 
GAGTGCCGAC TGCATCTGGA GGACTTCTTT GTCCCTCTGC TGACAAAGTT CCTTCGCAAC 
CCTGCCACCA TAAGCCAGGT GCTGGGCTTC AAGTTCACCT TCTACCAGGG CGAAGCGGGA 
GAGCCCACCC CAGCGTCCCT GTACCGGCTG GCCGCCCTCC TGATCAGGAA CGAGCTGATT 
GCCCTGGACG ACCTCTACTC CCTGCTGCTG CCCGACGACG GGGCCCTGTC GAGGCAGCAC 
AAGAAGGAGG TGGCGGCGGC TAAGACGTAC GCCCGGAGAG CGACCGTGGT GGTGGTCTCG 
TCGGACAAGA AGGACGAGGA GAAGAAGGAG GAGGACAAGG ACGACGACCT GACCCTGGAG 
GAGAACCAGA AGCTGGGCCT GTGTGAGGCC CTGCTGGAAG TGGGCGACTG GCCCAACGCC 
CAAAAGCTGA TGGCGCGCCT GCCCGAGCAC TACGCCGTGT CGCAGCCCCA CATTGCCCGG 
CGCCTCTGCC TCCTGGTCCA CACCCTGGTG GAGCCCTTGT ACCGCAGGCA CAGCGGGCTG 
CCGGAGGGCT TCCGGCGCTC GTACGCGTCC TCCCGTCGGG GGGGCCTGCG GCCGCCCGTC 
CTCGTGGAGA CCTTCGGGGA CTTCCAGGCA CGCGCCCTGC CCATGCTCGT GGCCCTGGGC 
CCCATGCTGC ACCTGGACCC TGTGCTCATC GTCAAGGTGG TGCGCCTGGC CAGAGCGCAC 
CTGCACAAGA CCAGCTCCTC GGCGGCTGGA GACCTGCGGT TCGACTTGCT CACCCTGGTC 
GGGGAGAGCC TGCTGCCCTC CCTGTCCCTT CTCGACTGCA ACTGCTGCGT GGCCGACGAG 
ATCTGGTCCC TGCTCAAGCT CTACCCCTAC CAGCAGAGGT ACCGTCTGTA CAGCCAGTGG 
AAGAACGAGG CGTACGTGAG TCACCCGTTG CTCATTCGGG TGAAGGCGGA CTCGCTCAAG 
AGGATCAAGT ACATCATGAA GAGACTCAGC AAGGAAAATG TGAAGCCTTC GGGGCGACAG 
ATTGGCAAGC TCAGCCACAG CAACCCTTGC TTTCTCTTTG ACTATATCCT GTCCCAGATC 
CAGACCTGGG ACAACCTGAT CGTGCCCGTG GTGGACTCCC TCAAGTACCT CACCATGCTC 
TCCTACGACG TTCTCGCCTA CTGCGTGGCG GAGGCGCTCT GCAATCCGGA GAAGGACCGC 
ATGAAGCATG ACGGCACCAG CATCTCCCTC TGGCTGCAGA GTCTGGCCAG TTTCTGTGGC 
GCCGTCTTCA AGAAGTACTC CATCGACCTG ACGGGACTGC TGCAGCTCGT GGCAAATCAG 
CTGAAGGCAG AAAAAAGCCT TGACCTGCTG GTGCTGAAGG ACATCGTACA GAAGATGACC 
GGCATCGAGT CGACGGAACA GGCAACGCAG GACCAACTGG AGGCGATGTG CGGAGGCGAA 
CTGCTTAAGG CCGAGGGCGG CTACTTCCAC CAGCTGCGCA ACACCAAGAA GTCGTCCCAG 
CGGCTGAAGG AGGCCCTGCT GGAGCAGGAC CTGGCCCTGC CGCTCTGCCT GCTCATGGCC 
CAGCAGAAGA ACTGCATCCT CTACCGGGAC CAGGAGGCCT CCCACCTCAA GCTGGTTGGA 
AAGCTCTACG ACCAGTGTCA GGACACCCTG GTCCAGTTTG GCAGCTTCCT GTCGAGCAGC 
CTGAGCACCG AAGAGTACGC CGGCCGCCTG CCGCCCGTGG ACCAGCTACT CTCGCGGTTC 
CACGTGCAGG CCGACGTGGC CTTCTTTTTG GCCAGGCCCA TGTTCGGACA TTCCATCGCG 
CTCAAGTTCG ACGACGCCAA GAGGAGGGAC AAGACGTTCA AAAGTCTAAC CGCCGCGCAG 
AAGTTGCAGC GCTACGTCGA CGCGGTGGAC CAGGTGATGT CGCGGGTCGT CGAGAGTGTG 
CGGCCGCTGC ACCCGTCCAA GACGTGGGAG GACCTCAGCC CGCAGTTCTA CGTCACCTTC 
TGGTCGCTCT CGATGTACGA CCTCCACGTG CCCACGTCCA GCTACGAGAG GGAGGTGGGC 
AAGTTGAAGC AGCAGGTGGT GCAGGCGGAG GACAGCAAGG ACATGGTGCC CAGCAAGCGC 
AAGAAGGAGC GCGACCGGTG CGAAGCGCTC ATGGAGAAGC TGCAGGAGGA GGAGCGCAAG 
CAGCAGGACC ACTGCGGGCG CGTCCTGGCA CGGCTGCGCA GCGAGAAGGA TGCCTGGTTC 
CACTCCCGTT CGGCCAAGAA CGAGACCATC ACGCAGTTCC TGCAGCTGTG CGTGTTCCCG 
CGCTGCACGT TCACGGCCCT GGACGCCCTC TACTGTGCCA AGTTTGTGCA CCTGATCCAC 
ATCCTGAAGA CGCCAAACTT CTCCACCCTC ATCTGCTACG ACAAGATCTT CTGCGACATC 
ACTTACACAG TGACGGCATG CACGGAGAAC GAAGCGAACC GCTACGGGCG CTTCCTCTGC 
TCCATGCTGG AGACGGTGAT GCGGTGGCAC AGCGACAAGG CCATCTTTGA CAAGGAGTGT 
GCCAACTTCC CCGGCTTCAT GACCAAGTTC CGGGGCGCCA GCCAGTCGTC GGACATTGCC 
ACGGACCACG TGGACTACGA GAACTACCGG CACGTCTGTC ACAAGTGGCA CTTCAAGATC 
ACTAAGGCCC TGGTCATCTG TCTGGAGTCT GGGGACTACG TCCAGATCCG GAACAGCCTG 
ATTGTGCTCA CCAAGGTGCT GCCCCACTTC CCGGTCATGA CCAACTTGGG CCAGGCGCTG 
GAGCGGCGCA TCGAGAAGAT CCGCCAGGAG GAGAAGGAGA AGAGGCCGGA CCTCTTTGCT 
CTTGCCACCG GCTACGCCGG CCAGCTGAAG GGCAAGAAAT CCGAGCTGAT CCCGGAGCAC 
GAGTTCCACC TCAAGGACAG CAAAGACGGC CGCGCGGTGA GCGCCACCGC ACAGGTGAAG 
AGCGCTGCAG ATCACACGGT GACGACGGCG GCACATGCCG CCACCTCTTC CCAAGTGGCG 
AGTGTCGATG GTGCCAAGCA GAAGCGGGGA GACTCCGTGA GGGAGTCCAA CTCGTCACCA 
GCACTGTCCG GCAATTCTTC TGGGAAGCTG TCGAGCAGCG GCGGAGGTCT CGTCCAGTCT 
TCGTCTTCGT CGTCGTCGTC CAAGATGACA TCGTCCAGTA GTTCTTCCGC CACCCGCAGG 
GAGTCCAACA GCTTTGGTGC GGTTGTGGAG AGTGCCACGA CGGCCGCTTT TCCTCGGAGG 
TCGCTGAGCA AGGAGTCCGG GAGCGGGGAC GGACACGACA AGGGCGCCGC CACCGACCGC 
AAGGGCAGCT CGCTGTCCTC CCTCCACGAG CAGGACGTCA GGCCCTCCAA GGACGACGTC 
CACAAAAATG ACCACTGGCT GGAGCCCGGG GACGCGCCCG GAGACCACAA GCGGCGGCGC 
GTGGACGGGG GCAGCAGCAG CGGCGGCAGC AGCAGTGGCA AGGTGGGAGG CAGTCCCCGG 
GGCCGTAGCC TCGGGGGCAC GCAGAGCCCC AAGGTCTCGG AACCCGAGGG GAAGTCTGAG 
GACCGCGGGG GAGGGGGGGA CCGCCCTTCT GAGAAGTGGG CTCACCCCAA GGACTCCCAC 
GACCCGGCCA CGGGGGACAA GGGCAAGGCC AAGAAGGGCA GCCGCAAGCG GGACCACTCC 
GACGAGCCCC CGCTCGAGGG CAAGCGCAAG AAGGACGAGG ACGCCTTGAA GAGCTCGAGC 
AAGCGCAACG GGGAGGACTC TAAGGAGAAG GAGCGGTACC GGAGGGTGAG AGAGCAGCAG 
CCCCTGCTGC TGGGGCTCCC TCGTGACCAG CTGGTCGCAC TCTCTCCTCA GGAAGAGGAC 
AAGCTGCGAG AGTCCCCCAA GCTGCGAGAG CGCAGGGCCG ACCGGGACGA CAAGAAGCAT 
CATCGATCGA GCAGCAGCAG TGGCAGCTCG ACGTCAACGA AAAAGCGATC ATCCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029843669.1
CDS	13..4632
Translation	MAGRSVPLDICKSWERTGKSDFVKLCRSAASCMQTSRLPFDKGGKEMQLLLHDLCWHVLEDRLKVEQAVSALAEVTALHQELPSMLADVIFLLDMETLSVENRDQRDRFYWLVGGCAKVVPDSLLKERLDIETLGDGGIVKNSRVMLTKFVKIKTRLFYKQNKYNLFREESEGYAKLTTELSQEITSKISPDYMIEVMRSLIGCFNLDPNRVLDVVLEAFECRLHLEDFFVPLLTKFLRNPATISQVLGFKFTFYQGEAGEPTPASLYRLAALLIRNELIALDDLYSLLLPDDGALSRQHKKEVAAAKTYARRATVVVVSSDKKDEEKKEEDKDDDLTLEENQKLGLCEALLEVGDWPNAQKLMARLPEHYAVSQPHIARRLCLLVHTLVEPLYRRHSGLPEGFRRSYASSRRGGLRPPVLVETFGDFQARALPMLVALGPMLHLDPVLIVKVVRLARAHLHKTSSSAAGDLRFDLLTLVGESLLPSLSLLDCNCCVADEIWSLLKLYPYQQRYRLYSQWKNEAYVSHPLLIRVKADSLKRIKYIMKRLSKENVKPSGRQIGKLSHSNPCFLFDYILSQIQTWDNLIVPVVDSLKYLTMLSYDVLAYCVAEALCNPEKDRMKHDGTSISLWLQSLASFCGAVFKKYSIDLTGLLQLVANQLKAEKSLDLLVLKDIVQKMTGIESTEQATQDQLEAMCGGELLKAEGGYFHQLRNTKKSSQRLKEALLEQDLALPLCLLMAQQKNCILYRDQEASHLKLVGKLYDQCQDTLVQFGSFLSSSLSTEEYAGRLPPVDQLLSRFHVQADVAFFLARPMFGHSIALKFDDAKRRDKTFKSLTAAQKLQRYVDAVDQVMSRVVESVRPLHPSKTWEDLSPQFYVTFWSLSMYDLHVPTSSYEREVGKLKQQVVQAEDSKDMVPSKRKKERDRCEALMEKLQEEERKQQDHCGRVLARLRSEKDAWFHSRSAKNETITQFLQLCVFPRCTFTALDALYCAKFVHLIHILKTPNFSTLICYDKIFCDITYTVTACTENEANRYGRFLCSMLETVMRWHSDKAIFDKECANFPGFMTKFRGASQSSDIATDHVDYENYRHVCHKWHFKITKALVICLESGDYVQIRNSLIVLTKVLPHFPVMTNLGQALERRIEKIRQEEKEKRPDLFALATGYAGQLKGKKSELIPEHEFHLKDSKDGRAVSATAQVKSAADHTVTTAAHAATSSQVASVDGAKQKRGDSVRESNSSPALSGNSSGKLSSSGGGLVQSSSSSSSSKMTSSSSSSATRRESNSFGAVVESATTAAFPRRSLSKESGSGDGHDKGAATDRKGSSLSSLHEQDVRPSKDDVHKNDHWLEPGDAPGDHKRRRVDGGSSSGGSSSGKVGGSPRGRSLGGTQSPKVSEPEGKSEDRGGGGDRPSEKWAHPKDSHDPATGDKGKAKKGSRKRDHSDEPPLEGKRKKDEDALKSSSKRNGEDSKEKERYRRVREQQPLLLGLPRDQLVALSPQEEDKLRESPKLRERRADRDDKKHHRSSSSSGSSTSTKKRSSF

Target ORF information:

RefSeq Version	XM_029987809.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029987809.1

ORF Insert Sequence:

ATGGCGGGCC GGTCTGTACC GTTGGACATT TGTAAAAGTT GGGAAAGGAC GGGGAAATCG 
GACTTTGTCA AACTATGTCG CTCAGCGGCA TCATGCATGC AGACGTCGAG GCTGCCTTTC 
GACAAGGGTG GAAAAGAGAT GCAGCTTCTG TTACACGACC TCTGCTGGCA CGTGCTGGAG 
GATCGCCTGA AAGTTGAGCA AGCTGTGTCG GCGCTGGCCG AAGTGACTGC CTTGCATCAG 
GAGCTGCCCT CGATGCTGGC AGACGTCATC TTTCTGCTGG ACATGGAGAC GCTGTCGGTG 
GAGAACCGTG ACCAGAGGGA CCGTTTTTAC TGGCTCGTCG GCGGCTGTGC AAAGGTTGTT 
CCCGACTCGC TGCTGAAGGA GCGGCTGGAC ATCGAGACCT TGGGAGACGG CGGGATCGTG 
AAGAACAGCC GTGTCATGCT CACCAAGTTT GTCAAGATCA AGACACGACT GTTCTACAAG 
CAGAACAAGT ACAACCTGTT CCGGGAGGAG AGCGAGGGCT ACGCCAAGCT GACCACGGAG 
CTGAGCCAGG AGATCACCAG CAAGATCAGC CCCGATTACA TGATAGAAGT GATGCGCTCG 
CTCATTGGCT GCTTCAATCT GGACCCCAAC CGCGTGTTGG ACGTGGTTCT GGAGGCGTTT 
GAGTGCCGAC TGCATCTGGA GGACTTCTTT GTCCCTCTGC TGACAAAGTT CCTTCGCAAC 
CCTGCCACCA TAAGCCAGGT GCTGGGCTTC AAGTTCACCT TCTACCAGGG CGAAGCGGGA 
GAGCCCACCC CAGCGTCCCT GTACCGGCTG GCCGCCCTCC TGATCAGGAA CGAGCTGATT 
GCCCTGGACG ACCTCTACTC CCTGCTGCTG CCCGACGACG GGGCCCTGTC GAGGCAGCAC 
AAGAAGGAGG TGGCGGCGGC TAAGACGTAC GCCCGGAGAG CGACCGTGGT GGTGGTCTCG 
TCGGACAAGA AGGACGAGGA GAAGAAGGAG GAGGACAAGG ACGACGACCT GACCCTGGAG 
GAGAACCAGA AGCTGGGCCT GTGTGAGGCC CTGCTGGAAG TGGGCGACTG GCCCAACGCC 
CAAAAGCTGA TGGCGCGCCT GCCCGAGCAC TACGCCGTGT CGCAGCCCCA CATTGCCCGG 
CGCCTCTGCC TCCTGGTCCA CACCCTGGTG GAGCCCTTGT ACCGCAGGCA CAGCGGGCTG 
CCGGAGGGCT TCCGGCGCTC GTACGCGTCC TCCCGTCGGG GGGGCCTGCG GCCGCCCGTC 
CTCGTGGAGA CCTTCGGGGA CTTCCAGGCA CGCGCCCTGC CCATGCTCGT GGCCCTGGGC 
CCCATGCTGC ACCTGGACCC TGTGCTCATC GTCAAGGTGG TGCGCCTGGC CAGAGCGCAC 
CTGCACAAGA CCAGCTCCTC GGCGGCTGGA GACCTGCGGT TCGACTTGCT CACCCTGGTC 
GGGGAGAGCC TGCTGCCCTC CCTGTCCCTT CTCGACTGCA ACTGCTGCGT GGCCGACGAG 
ATCTGGTCCC TGCTCAAGCT CTACCCCTAC CAGCAGAGGT ACCGTCTGTA CAGCCAGTGG 
AAGAACGAGG CGTACGTGAG TCACCCGTTG CTCATTCGGG TGAAGGCGGA CTCGCTCAAG 
AGGATCAAGT ACATCATGAA GAGACTCAGC AAGGAAAATG TGAAGCCTTC GGGGCGACAG 
ATTGGCAAGC TCAGCCACAG CAACCCTTGC TTTCTCTTTG ACTATATCCT GTCCCAGATC 
CAGACCTGGG ACAACCTGAT CGTGCCCGTG GTGGACTCCC TCAAGTACCT CACCATGCTC 
TCCTACGACG TTCTCGCCTA CTGCGTGGCG GAGGCGCTCT GCAATCCGGA GAAGGACCGC 
ATGAAGCATG ACGGCACCAG CATCTCCCTC TGGCTGCAGA GTCTGGCCAG TTTCTGTGGC 
GCCGTCTTCA AGAAGTACTC CATCGACCTG ACGGGACTGC TGCAGCTCGT GGCAAATCAG 
CTGAAGGCAG AAAAAAGCCT TGACCTGCTG GTGCTGAAGG ACATCGTACA GAAGATGACC 
GGCATCGAGT CGACGGAACA GGCAACGCAG GACCAACTGG AGGCGATGTG CGGAGGCGAA 
CTGCTTAAGG CCGAGGGCGG CTACTTCCAC CAGCTGCGCA ACACCAAGAA GTCGTCCCAG 
CGGCTGAAGG AGGCCCTGCT GGAGCAGGAC CTGGCCCTGC CGCTCTGCCT GCTCATGGCC 
CAGCAGAAGA ACTGCATCCT CTACCGGGAC CAGGAGGCCT CCCACCTCAA GCTGGTTGGA 
AAGCTCTACG ACCAGTGTCA GGACACCCTG GTCCAGTTTG GCAGCTTCCT GTCGAGCAGC 
CTGAGCACCG AAGAGTACGC CGGCCGCCTG CCGCCCGTGG ACCAGCTACT CTCGCGGTTC 
CACGTGCAGG CCGACGTGGC CTTCTTTTTG GCCAGGCCCA TGTTCGGACA TTCCATCGCG 
CTCAAGTTCG ACGACGCCAA GAGGAGGGAC AAGACGTTCA AAAGTCTAAC CGCCGCGCAG 
AAGTTGCAGC GCTACGTCGA CGCGGTGGAC CAGGTGATGT CGCGGGTCGT CGAGAGTGTG 
CGGCCGCTGC ACCCGTCCAA GACGTGGGAG GACCTCAGCC CGCAGTTCTA CGTCACCTTC 
TGGTCGCTCT CGATGTACGA CCTCCACGTG CCCACGTCCA GCTACGAGAG GGAGGTGGGC 
AAGTTGAAGC AGCAGGTGGT GCAGGCGGAG GACAGCAAGG ACATGGTGCC CAGCAAGCGC 
AAGAAGGAGC GCGACCGGTG CGAAGCGCTC ATGGAGAAGC TGCAGGAGGA GGAGCGCAAG 
CAGCAGGACC ACTGCGGGCG CGTCCTGGCA CGGCTGCGCA GCGAGAAGGA TGCCTGGTTC 
CACTCCCGTT CGGCCAAGAA CGAGACCATC ACGCAGTTCC TGCAGCTGTG CGTGTTCCCG 
CGCTGCACGT TCACGGCCCT GGACGCCCTC TACTGTGCCA AGTTTGTGCA CCTGATCCAC 
ATCCTGAAGA CGCCAAACTT CTCCACCCTC ATCTGCTACG ACAAGATCTT CTGCGACATC 
ACTTACACAG TGACGGCATG CACGGAGAAC GAAGCGAACC GCTACGGGCG CTTCCTCTGC 
TCCATGCTGG AGACGGTGAT GCGGTGGCAC AGCGACAAGG CCATCTTTGA CAAGGAGTGT 
GCCAACTTCC CCGGCTTCAT GACCAAGTTC CGGGGCGCCA GCCAGTCGTC GGACATTGCC 
ACGGACCACG TGGACTACGA GAACTACCGG CACGTCTGTC ACAAGTGGCA CTTCAAGATC 
ACTAAGGCCC TGGTCATCTG TCTGGAGTCT GGGGACTACG TCCAGATCCG GAACAGCCTG 
ATTGTGCTCA CCAAGGTGCT GCCCCACTTC CCGGTCATGA CCAACTTGGG CCAGGCGCTG 
GAGCGGCGCA TCGAGAAGAT CCGCCAGGAG GAGAAGGAGA AGAGGCCGGA CCTCTTTGCT 
CTTGCCACCG GCTACGCCGG CCAGCTGAAG GGCAAGAAAT CCGAGCTGAT CCCGGAGCAC 
GAGTTCCACC TCAAGGACAG CAAAGACGGC CGCGCGGTGA GCGCCACCGC ACAGGTGAAG 
AGCGCTGCAG ATCACACGGT GACGACGGCG GCACATGCCG CCACCTCTTC CCAAGTGGCG 
AGTGTCGATG GTGCCAAGCA GAAGCGGGGA GACTCCGTGA GGGAGTCCAA CTCGTCACCA 
GCACTGTCCG GCAATTCTTC TGGGAAGCTG TCGAGCAGCG GCGGAGGTCT CGTCCAGTCT 
TCGTCTTCGT CGTCGTCGTC CAAGATGACA TCGTCCAGTA GTTCTTCCGC CACCCGCAGG 
GAGTCCAACA GCTTTGGTGC GGTTGTGGAG AGTGCCACGA CGGCCGCTTT TCCTCGGAGG 
TCGCTGAGCA AGGAGTCCGG GAGCGGGGAC GGACACGACA AGGGCGCCGC CACCGACCGC 
AAGGGCAGCT CGCTGTCCTC CCTCCACGAG CAGGACGTCA GGCCCTCCAA GGACGACGTC 
CACAAAAATG ACCACTGGCT GGAGCCCGGG GACGCGCCCG GAGACCACAA GCGGCGGCGC 
GTGGACGGGG GCAGCAGCAG CGGCGGCAGC AGCAGTGGCA AGGTGGGAGG CAGTCCCCGG 
GGCCGTAGCC TCGGGGGCAC GCAGAGCCCC AAGGTCTCGG AACCCGAGGG GAAGTCTGAG 
GACCGCGGGG GAGGGGGGGA CCGCCCTTCT GAGAAGTGGG CTCACCCCAA GGACTCCCAC 
GACCCGGCCA CGGGGGACAA GGGCAAGGCC AAGAAGGGCA GCCGCAAGCG GGACCACTCC 
GACGAGCCCC CGCTCGAGGG CAAGCGCAAG AAGGACGAGG ACGCCTTGAA GAGCTCGAGC 
AAGCGCAACG GGGAGGACTC TAAGGAGAAG GAGCGGTACC GGAGGGTGAG AGAGCAGCAG 
CCCCTGCTGC TGGGGCTCCC TCGTGACCAG CTGGTCGCAC TCTCTCCTCA GGAAGAGGAC 
AAGCTGCGAG AGTCCCCCAA GCTGCGAGAG CGCAGGGCCG ACCGGGACGA CAAGAAGCAT 
CATCGATCGA GCAGCAGCAG TGGCAGCTCG ACGTCAACGA AAAAGCGATC ATCCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8029916 ( XM_029987810.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029843670.1 Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X3, mRNA.

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CloneID	OIa29768
Clone ID Related Accession (Same CDS sequence)	XM_029987810.1
Accession Version	XM_029987810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	THO complex subunit 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021921043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGCC GGTCTGTACC GTTGGACATT TGTAAAAGTT GGGAAAGGAC GGGGAAATCG 
GACTTTGTCA AACTATGTCG CTCAGCGGCA TCATGCATGC AGACGTCGAG GCTGCCTTTC 
GACAAGGGTG GAAAAGAGAT GCAGCTTCTG TTACACGACC TCTGCTGGCA CGTGCTGGAG 
GATCGCCTGA AAGTTGAGCA AGCTGTGTCG GCGCTGGCCG AAGTGACTGC CTTGCATCAG 
GAGCTGCCCT CGATGCTGGC AGACGTCATC TTTCTGCTGG ACATGGAGAC GCTGTCGGTG 
GAGAACCGTG ACCAGAGGGA CCGTTTTTAC TGGCTCGTCG GCGGCTGTGC AAAGGTTGTT 
CCCGACTCGC TGCTGAAGGA GCGGCTGGAC ATCGAGACCT TGGGAGACGG CGGGATCGTG 
AAGAACAGCC GTGTCATGCT CACCAAGTTT GTCAAGATCA AGACACGACT GTTCTACAAG 
CAGAACAAGT ACAACCTGTT CCGGGAGGAG AGCGAGGGCT ACGCCAAGCT GACCACGGAG 
CTGAGCCAGG AGATCACCAG CAAGATCAGC CCCGATTACA TGATAGAAGT GATGCGCTCG 
CTCATTGGCT GCTTCAATCT GGACCCCAAC CGCGTGTTGG ACGTGGTTCT GGAGGCGTTT 
GAGTGCCGAC TGCATCTGGA GGACTTCTTT GTCCCTCTGC TGACAAAGTT CCTTCGCAAC 
CCTGCCACCA TAAGCCAGGT GCTGGGCTTC AAGTTCACCT TCTACCAGGT GAGGGGCGAA 
GCGGGAGAGC CCACCCCAGC GTCCCTGTAC CGGCTGGCCG CCCTCCTGAT CAGGAACGAG 
CTGATTGCCC TGGACGACCT CTACTCCCTG CTGCTGCCCG ACGACGGGGC CCTGTCGAGG 
CAGCACAAGA AGGAGGTGGC GGCGGCTAAG ACGTACGCCC GGAGAGCGAC CGTGGTGGTG 
GTCTCGTCGG ACAAGAAGGA CGAGGAGAAG AAGGAGGAGG ACAAGGACGA CGACCTGACC 
CTGGAGGAGA ACCAGAAGCT GGGCCTGTGT GAGGCCCTGC TGGAAGTGGG CGACTGGCCC 
AACGCCCAAA AGCTGATGGC GCGCCTGCCC GAGCACTACG CCGTGTCGCA GCCCCACATT 
GCCCGGCGCC TCTGCCTCCT GGTCCACACC CTGGTGGAGC CCTTGTACCG CAGGCACAGC 
GGGCTGCCGG AGGGCTTCCG GCGCTCGTAC GCGTCCTCCC GTCGGGGGGG CCTGCGGCCG 
CCCGTCCTCG TGGAGACCTT CGGGGACTTC CAGGCACGCG CCCTGCCCAT GCTCGTGGCC 
CTGGGCCCCA TGCTGCACCT GGACCCTGTG CTCATCGTCA AGGTGGTGCG CCTGGCCAGA 
GCGCACCTGC ACAAGACCAG CTCCTCGGCG GCTGGAGACC TGCGGTTCGA CTTGCTCACC 
CTGGTCGGGG AGAGCCTGCT GCCCTCCCTG TCCCTTCTCG ACTGCAACTG CTGCGTGGCC 
GACGAGATCT GGTCCCTGCT CAAGCTCTAC CCCTACCAGC AGAGGTACCG TCTGTACAGC 
CAGTGGAAGA ACGAGGCGTA CGTGAGTCAC CCGTTGCTCA TTCGGGTGAA GGCGGACTCG 
CTCAAGAGGA TCAAGTACAT CATGAAGAGA CTCAGCAAGG AAAATGTGAA GCCTTCGGGG 
CGACAGATTG GCAAGCTCAG CCACAGCAAC CCTTGCTTTC TCTTTGACTA TATCCTGTCC 
CAGATCCAGA CCTGGGACAA CCTGATCGTG CCCGTGGTGG ACTCCCTCAA GTACCTCACC 
ATGCTCTCCT ACGACGTTCT CGCCTACTGC GTGGCGGAGG CGCTCTGCAA TCCGGAGAAG 
GACCGCATGA AGCATGACGG CACCAGCATC TCCCTCTGGC TGCAGAGTCT GGCCAGTTTC 
TGTGGCGCCG TCTTCAAGAA GTACTCCATC GACCTGACGG GACTGCTGCA GCTCGTGGCA 
AATCAGCTGA AGGCAGAAAA AAGCCTTGAC CTGCTGGTGC TGAAGGACAT CGTACAGAAG 
ATGACCGGCA TCGAGTCGAC GGAACAGGCA ACGCAGGACC AACTGGAGGC GATGTGCGGA 
GGCGAACTGC TTAAGGCCGA GGGCGGCTAC TTCCACCAGC TGCGCAACAC CAAGAAGTCG 
TCCCAGCGGC TGAAGGAGGC CCTGCTGGAG CAGGACCTGG CCCTGCCGCT CTGCCTGCTC 
ATGGCCCAGC AGAAGAACTG CATCCTCTAC CGGGACCAGG AGGCCTCCCA CCTCAAGCTG 
GTTGGAAAGC TCTACGACCA GTGTCAGGAC ACCCTGGTCC AGTTTGGCAG CTTCCTGTCG 
AGCAGCCTGA GCACCGAAGA GTACGCCGGC CGCCTGCCGC CCGTGGACCA GCTACTCTCG 
CGGTTCCACG TGCAGGCCGA CGTGGCCTTC TTTTTGGCCA GGCCCATGTT CGGACATTCC 
ATCGCGCTCA AGTTCGACGA CGCCAAGAGG AGGGACAAGA CGTTCAAAAG TCTAACCGCC 
GCGCAGAAGC GCTACGTCGA CGCGGTGGAC CAGGTGATGT CGCGGGTCGT CGAGAGTGTG 
CGGCCGCTGC ACCCGTCCAA GACGTGGGAG GACCTCAGCC CGCAGTTCTA CGTCACCTTC 
TGGTCGCTCT CGATGTACGA CCTCCACGTG CCCACGTCCA GCTACGAGAG GGAGGTGGGC 
AAGTTGAAGC AGCAGGTGGT GCAGGCGGAG GACAGCAAGG ACATGGTGCC CAGCAAGCGC 
AAGAAGGAGC GCGACCGGTG CGAAGCGCTC ATGGAGAAGC TGCAGGAGGA GGAGCGCAAG 
CAGCAGGACC ACTGCGGGCG CGTCCTGGCA CGGCTGCGCA GCGAGAAGGA TGCCTGGTTC 
CACTCCCGTT CGGCCAAGAA CGAGACCATC ACGCAGTTCC TGCAGCTGTG CGTGTTCCCG 
CGCTGCACGT TCACGGCCCT GGACGCCCTC TACTGTGCCA AGTTTGTGCA CCTGATCCAC 
ATCCTGAAGA CGCCAAACTT CTCCACCCTC ATCTGCTACG ACAAGATCTT CTGCGACATC 
ACTTACACAG TGACGGCATG CACGGAGAAC GAAGCGAACC GCTACGGGCG CTTCCTCTGC 
TCCATGCTGG AGACGGTGAT GCGGTGGCAC AGCGACAAGG CCATCTTTGA CAAGGAGTGT 
GCCAACTTCC CCGGCTTCAT GACCAAGTTC CGGGGCGCCA GCCAGTCGTC GGACATTGCC 
ACGGACCACG TGGACTACGA GAACTACCGG CACGTCTGTC ACAAGTGGCA CTTCAAGATC 
ACTAAGGCCC TGGTCATCTG TCTGGAGTCT GGGGACTACG TCCAGATCCG GAACAGCCTG 
ATTGTGCTCA CCAAGGTGCT GCCCCACTTC CCGGTCATGA CCAACTTGGG CCAGGCGCTG 
GAGCGGCGCA TCGAGAAGAT CCGCCAGGAG GAGAAGGAGA AGAGGCCGGA CCTCTTTGCT 
CTTGCCACCG GCTACGCCGG CCAGCTGAAG GGCAAGAAAT CCGAGCTGAT CCCGGAGCAC 
GAGTTCCACC TCAAGGACAG CAAAGACGGC CGCGCGGTGA GCGCCACCGC ACAGGTGAAG 
AGCGCTGCAG ATCACACGGT GACGACGGCG GCACATGCCG CCACCTCTTC CCAAGTGGCG 
AGTGTCGATG GTGCCAAGCA GAAGCGGGGA GACTCCGTGA GGGAGTCCAA CTCGTCACCA 
GCACTGTCCG GCAATTCTTC TGGGAAGCTG TCGAGCAGCG GCGGAGGTCT CGTCCAGTCT 
TCGTCTTCGT CGTCGTCGTC CAAGATGACA TCGTCCAGTA GTTCTTCCGC CACCCGCAGG 
GAGTCCAACA GCTTTGGTGC GGTTGTGGAG AGTGCCACGA CGGCCGCTTT TCCTCGGAGG 
TCGCTGAGCA AGGAGTCCGG GAGCGGGGAC GGACACGACA AGGGCGCCGC CACCGACCGC 
AAGGGCAGCT CGCTGTCCTC CCTCCACGAG CAGGACGTCA GGCCCTCCAA GGACGACGTC 
CACAAAAATG ACCACTGGCT GGAGCCCGGG GACGCGCCCG GAGACCACAA GCGGCGGCGC 
GTGGACGGGG GCAGCAGCAG CGGCGGCAGC AGCAGTGGCA AGGTGGGAGG CAGTCCCCGG 
GGCCGTAGCC TCGGGGGCAC GCAGAGCCCC AAGGTCTCGG AACCCGAGGG GAAGTCTGAG 
GACCGCGGGG GAGGGGGGGA CCGCCCTTCT GAGAAGTGGG CTCACCCCAA GGACTCCCAC 
GACCCGGCCA CGGGGGACAA GGGCAAGGCC AAGAAGGGCA GCCGCAAGCG GGACCACTCC 
GACGAGCCCC CGCTCGAGGG CAAGCGCAAG AAGGACGAGG ACGCCTTGAA GAGCTCGAGC 
AAGCGCAACG GGGAGGACTC TAAGGAGAAG GAGCGGTACC GGAGGGTGAG AGAGCAGCAG 
CCCCTGCTGC TGGGGCTCCC TCGTGACCAG CTGGTCGCAC TCTCTCCTCA GGAAGAGGAC 
AAGCTGCGAG AGTCCCCCAA GCTGCGAGAG CGCAGGGCCG ACCGGGACGA CAAGAAGCAT 
CATCGATCGA GCAGCAGCAG TGGCAGCTCG ACGTCAACGA AAAAGCGATC ATCCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029843670.1
CDS	13..4632
Translation	MAGRSVPLDICKSWERTGKSDFVKLCRSAASCMQTSRLPFDKGGKEMQLLLHDLCWHVLEDRLKVEQAVSALAEVTALHQELPSMLADVIFLLDMETLSVENRDQRDRFYWLVGGCAKVVPDSLLKERLDIETLGDGGIVKNSRVMLTKFVKIKTRLFYKQNKYNLFREESEGYAKLTTELSQEITSKISPDYMIEVMRSLIGCFNLDPNRVLDVVLEAFECRLHLEDFFVPLLTKFLRNPATISQVLGFKFTFYQVRGEAGEPTPASLYRLAALLIRNELIALDDLYSLLLPDDGALSRQHKKEVAAAKTYARRATVVVVSSDKKDEEKKEEDKDDDLTLEENQKLGLCEALLEVGDWPNAQKLMARLPEHYAVSQPHIARRLCLLVHTLVEPLYRRHSGLPEGFRRSYASSRRGGLRPPVLVETFGDFQARALPMLVALGPMLHLDPVLIVKVVRLARAHLHKTSSSAAGDLRFDLLTLVGESLLPSLSLLDCNCCVADEIWSLLKLYPYQQRYRLYSQWKNEAYVSHPLLIRVKADSLKRIKYIMKRLSKENVKPSGRQIGKLSHSNPCFLFDYILSQIQTWDNLIVPVVDSLKYLTMLSYDVLAYCVAEALCNPEKDRMKHDGTSISLWLQSLASFCGAVFKKYSIDLTGLLQLVANQLKAEKSLDLLVLKDIVQKMTGIESTEQATQDQLEAMCGGELLKAEGGYFHQLRNTKKSSQRLKEALLEQDLALPLCLLMAQQKNCILYRDQEASHLKLVGKLYDQCQDTLVQFGSFLSSSLSTEEYAGRLPPVDQLLSRFHVQADVAFFLARPMFGHSIALKFDDAKRRDKTFKSLTAAQKRYVDAVDQVMSRVVESVRPLHPSKTWEDLSPQFYVTFWSLSMYDLHVPTSSYEREVGKLKQQVVQAEDSKDMVPSKRKKERDRCEALMEKLQEEERKQQDHCGRVLARLRSEKDAWFHSRSAKNETITQFLQLCVFPRCTFTALDALYCAKFVHLIHILKTPNFSTLICYDKIFCDITYTVTACTENEANRYGRFLCSMLETVMRWHSDKAIFDKECANFPGFMTKFRGASQSSDIATDHVDYENYRHVCHKWHFKITKALVICLESGDYVQIRNSLIVLTKVLPHFPVMTNLGQALERRIEKIRQEEKEKRPDLFALATGYAGQLKGKKSELIPEHEFHLKDSKDGRAVSATAQVKSAADHTVTTAAHAATSSQVASVDGAKQKRGDSVRESNSSPALSGNSSGKLSSSGGGLVQSSSSSSSSKMTSSSSSSATRRESNSFGAVVESATTAAFPRRSLSKESGSGDGHDKGAATDRKGSSLSSLHEQDVRPSKDDVHKNDHWLEPGDAPGDHKRRRVDGGSSSGGSSSGKVGGSPRGRSLGGTQSPKVSEPEGKSEDRGGGGDRPSEKWAHPKDSHDPATGDKGKAKKGSRKRDHSDEPPLEGKRKKDEDALKSSSKRNGEDSKEKERYRRVREQQPLLLGLPRDQLVALSPQEEDKLRESPKLRERRADRDDKKHHRSSSSSGSSTSTKKRSSF

Target ORF information:

RefSeq Version	XM_029987810.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029987810.1

ORF Insert Sequence:

ATGGCGGGCC GGTCTGTACC GTTGGACATT TGTAAAAGTT GGGAAAGGAC GGGGAAATCG 
GACTTTGTCA AACTATGTCG CTCAGCGGCA TCATGCATGC AGACGTCGAG GCTGCCTTTC 
GACAAGGGTG GAAAAGAGAT GCAGCTTCTG TTACACGACC TCTGCTGGCA CGTGCTGGAG 
GATCGCCTGA AAGTTGAGCA AGCTGTGTCG GCGCTGGCCG AAGTGACTGC CTTGCATCAG 
GAGCTGCCCT CGATGCTGGC AGACGTCATC TTTCTGCTGG ACATGGAGAC GCTGTCGGTG 
GAGAACCGTG ACCAGAGGGA CCGTTTTTAC TGGCTCGTCG GCGGCTGTGC AAAGGTTGTT 
CCCGACTCGC TGCTGAAGGA GCGGCTGGAC ATCGAGACCT TGGGAGACGG CGGGATCGTG 
AAGAACAGCC GTGTCATGCT CACCAAGTTT GTCAAGATCA AGACACGACT GTTCTACAAG 
CAGAACAAGT ACAACCTGTT CCGGGAGGAG AGCGAGGGCT ACGCCAAGCT GACCACGGAG 
CTGAGCCAGG AGATCACCAG CAAGATCAGC CCCGATTACA TGATAGAAGT GATGCGCTCG 
CTCATTGGCT GCTTCAATCT GGACCCCAAC CGCGTGTTGG ACGTGGTTCT GGAGGCGTTT 
GAGTGCCGAC TGCATCTGGA GGACTTCTTT GTCCCTCTGC TGACAAAGTT CCTTCGCAAC 
CCTGCCACCA TAAGCCAGGT GCTGGGCTTC AAGTTCACCT TCTACCAGGT GAGGGGCGAA 
GCGGGAGAGC CCACCCCAGC GTCCCTGTAC CGGCTGGCCG CCCTCCTGAT CAGGAACGAG 
CTGATTGCCC TGGACGACCT CTACTCCCTG CTGCTGCCCG ACGACGGGGC CCTGTCGAGG 
CAGCACAAGA AGGAGGTGGC GGCGGCTAAG ACGTACGCCC GGAGAGCGAC CGTGGTGGTG 
GTCTCGTCGG ACAAGAAGGA CGAGGAGAAG AAGGAGGAGG ACAAGGACGA CGACCTGACC 
CTGGAGGAGA ACCAGAAGCT GGGCCTGTGT GAGGCCCTGC TGGAAGTGGG CGACTGGCCC 
AACGCCCAAA AGCTGATGGC GCGCCTGCCC GAGCACTACG CCGTGTCGCA GCCCCACATT 
GCCCGGCGCC TCTGCCTCCT GGTCCACACC CTGGTGGAGC CCTTGTACCG CAGGCACAGC 
GGGCTGCCGG AGGGCTTCCG GCGCTCGTAC GCGTCCTCCC GTCGGGGGGG CCTGCGGCCG 
CCCGTCCTCG TGGAGACCTT CGGGGACTTC CAGGCACGCG CCCTGCCCAT GCTCGTGGCC 
CTGGGCCCCA TGCTGCACCT GGACCCTGTG CTCATCGTCA AGGTGGTGCG CCTGGCCAGA 
GCGCACCTGC ACAAGACCAG CTCCTCGGCG GCTGGAGACC TGCGGTTCGA CTTGCTCACC 
CTGGTCGGGG AGAGCCTGCT GCCCTCCCTG TCCCTTCTCG ACTGCAACTG CTGCGTGGCC 
GACGAGATCT GGTCCCTGCT CAAGCTCTAC CCCTACCAGC AGAGGTACCG TCTGTACAGC 
CAGTGGAAGA ACGAGGCGTA CGTGAGTCAC CCGTTGCTCA TTCGGGTGAA GGCGGACTCG 
CTCAAGAGGA TCAAGTACAT CATGAAGAGA CTCAGCAAGG AAAATGTGAA GCCTTCGGGG 
CGACAGATTG GCAAGCTCAG CCACAGCAAC CCTTGCTTTC TCTTTGACTA TATCCTGTCC 
CAGATCCAGA CCTGGGACAA CCTGATCGTG CCCGTGGTGG ACTCCCTCAA GTACCTCACC 
ATGCTCTCCT ACGACGTTCT CGCCTACTGC GTGGCGGAGG CGCTCTGCAA TCCGGAGAAG 
GACCGCATGA AGCATGACGG CACCAGCATC TCCCTCTGGC TGCAGAGTCT GGCCAGTTTC 
TGTGGCGCCG TCTTCAAGAA GTACTCCATC GACCTGACGG GACTGCTGCA GCTCGTGGCA 
AATCAGCTGA AGGCAGAAAA AAGCCTTGAC CTGCTGGTGC TGAAGGACAT CGTACAGAAG 
ATGACCGGCA TCGAGTCGAC GGAACAGGCA ACGCAGGACC AACTGGAGGC GATGTGCGGA 
GGCGAACTGC TTAAGGCCGA GGGCGGCTAC TTCCACCAGC TGCGCAACAC CAAGAAGTCG 
TCCCAGCGGC TGAAGGAGGC CCTGCTGGAG CAGGACCTGG CCCTGCCGCT CTGCCTGCTC 
ATGGCCCAGC AGAAGAACTG CATCCTCTAC CGGGACCAGG AGGCCTCCCA CCTCAAGCTG 
GTTGGAAAGC TCTACGACCA GTGTCAGGAC ACCCTGGTCC AGTTTGGCAG CTTCCTGTCG 
AGCAGCCTGA GCACCGAAGA GTACGCCGGC CGCCTGCCGC CCGTGGACCA GCTACTCTCG 
CGGTTCCACG TGCAGGCCGA CGTGGCCTTC TTTTTGGCCA GGCCCATGTT CGGACATTCC 
ATCGCGCTCA AGTTCGACGA CGCCAAGAGG AGGGACAAGA CGTTCAAAAG TCTAACCGCC 
GCGCAGAAGC GCTACGTCGA CGCGGTGGAC CAGGTGATGT CGCGGGTCGT CGAGAGTGTG 
CGGCCGCTGC ACCCGTCCAA GACGTGGGAG GACCTCAGCC CGCAGTTCTA CGTCACCTTC 
TGGTCGCTCT CGATGTACGA CCTCCACGTG CCCACGTCCA GCTACGAGAG GGAGGTGGGC 
AAGTTGAAGC AGCAGGTGGT GCAGGCGGAG GACAGCAAGG ACATGGTGCC CAGCAAGCGC 
AAGAAGGAGC GCGACCGGTG CGAAGCGCTC ATGGAGAAGC TGCAGGAGGA GGAGCGCAAG 
CAGCAGGACC ACTGCGGGCG CGTCCTGGCA CGGCTGCGCA GCGAGAAGGA TGCCTGGTTC 
CACTCCCGTT CGGCCAAGAA CGAGACCATC ACGCAGTTCC TGCAGCTGTG CGTGTTCCCG 
CGCTGCACGT TCACGGCCCT GGACGCCCTC TACTGTGCCA AGTTTGTGCA CCTGATCCAC 
ATCCTGAAGA CGCCAAACTT CTCCACCCTC ATCTGCTACG ACAAGATCTT CTGCGACATC 
ACTTACACAG TGACGGCATG CACGGAGAAC GAAGCGAACC GCTACGGGCG CTTCCTCTGC 
TCCATGCTGG AGACGGTGAT GCGGTGGCAC AGCGACAAGG CCATCTTTGA CAAGGAGTGT 
GCCAACTTCC CCGGCTTCAT GACCAAGTTC CGGGGCGCCA GCCAGTCGTC GGACATTGCC 
ACGGACCACG TGGACTACGA GAACTACCGG CACGTCTGTC ACAAGTGGCA CTTCAAGATC 
ACTAAGGCCC TGGTCATCTG TCTGGAGTCT GGGGACTACG TCCAGATCCG GAACAGCCTG 
ATTGTGCTCA CCAAGGTGCT GCCCCACTTC CCGGTCATGA CCAACTTGGG CCAGGCGCTG 
GAGCGGCGCA TCGAGAAGAT CCGCCAGGAG GAGAAGGAGA AGAGGCCGGA CCTCTTTGCT 
CTTGCCACCG GCTACGCCGG CCAGCTGAAG GGCAAGAAAT CCGAGCTGAT CCCGGAGCAC 
GAGTTCCACC TCAAGGACAG CAAAGACGGC CGCGCGGTGA GCGCCACCGC ACAGGTGAAG 
AGCGCTGCAG ATCACACGGT GACGACGGCG GCACATGCCG CCACCTCTTC CCAAGTGGCG 
AGTGTCGATG GTGCCAAGCA GAAGCGGGGA GACTCCGTGA GGGAGTCCAA CTCGTCACCA 
GCACTGTCCG GCAATTCTTC TGGGAAGCTG TCGAGCAGCG GCGGAGGTCT CGTCCAGTCT 
TCGTCTTCGT CGTCGTCGTC CAAGATGACA TCGTCCAGTA GTTCTTCCGC CACCCGCAGG 
GAGTCCAACA GCTTTGGTGC GGTTGTGGAG AGTGCCACGA CGGCCGCTTT TCCTCGGAGG 
TCGCTGAGCA AGGAGTCCGG GAGCGGGGAC GGACACGACA AGGGCGCCGC CACCGACCGC 
AAGGGCAGCT CGCTGTCCTC CCTCCACGAG CAGGACGTCA GGCCCTCCAA GGACGACGTC 
CACAAAAATG ACCACTGGCT GGAGCCCGGG GACGCGCCCG GAGACCACAA GCGGCGGCGC 
GTGGACGGGG GCAGCAGCAG CGGCGGCAGC AGCAGTGGCA AGGTGGGAGG CAGTCCCCGG 
GGCCGTAGCC TCGGGGGCAC GCAGAGCCCC AAGGTCTCGG AACCCGAGGG GAAGTCTGAG 
GACCGCGGGG GAGGGGGGGA CCGCCCTTCT GAGAAGTGGG CTCACCCCAA GGACTCCCAC 
GACCCGGCCA CGGGGGACAA GGGCAAGGCC AAGAAGGGCA GCCGCAAGCG GGACCACTCC 
GACGAGCCCC CGCTCGAGGG CAAGCGCAAG AAGGACGAGG ACGCCTTGAA GAGCTCGAGC 
AAGCGCAACG GGGAGGACTC TAAGGAGAAG GAGCGGTACC GGAGGGTGAG AGAGCAGCAG 
CCCCTGCTGC TGGGGCTCCC TCGTGACCAG CTGGTCGCAC TCTCTCCTCA GGAAGAGGAC 
AAGCTGCGAG AGTCCCCCAA GCTGCGAGAG CGCAGGGCCG ACCGGGACGA CAAGAAGCAT 
CATCGATCGA GCAGCAGCAG TGGCAGCTCG ACGTCAACGA AAAAGCGATC ATCCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8029916 ( XM_029987811.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029843671.1 Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X4, mRNA.

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CloneID	OIa29769
Clone ID Related Accession (Same CDS sequence)	XM_029987811.1
Accession Version	XM_029987811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	THO complex subunit 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021921043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGCC GGTCTGTACC GTTGGACATT TGTAAAAGTT GGGAAAGGAC GGGGAAATCG 
GACTTTGTCA AACTATGTCG CTCAGCGGCA TCATGCATGC AGACGTCGAG GCTGCCTTTC 
GACAAGGGTG GAAAAGAGAT GCAGCTTCTG TTACACGACC TCTGCTGGCA CGTGCTGGAG 
GATCGCCTGA AAGTTGAGCA AGCTGTGTCG GCGCTGGCCG AAGTGACTGC CTTGCATCAG 
GAGCTGCCCT CGATGCTGGC AGACGTCATC TTTCTGCTGG ACATGGAGAC GCTGTCGGTG 
GAGAACCGTG ACCAGAGGGA CCGTTTTTAC TGGCTCGTCG GCGGCTGTGC AAAGGTTGTT 
CCCGACTCGC TGCTGAAGGA GCGGCTGGAC ATCGAGACCT TGGGAGACGG CGGGATCGTG 
AAGAACAGCC GTGTCATGCT CACCAAGTTT GTCAAGATCA AGACACGACT GTTCTACAAG 
CAGAACAAGT ACAACCTGTT CCGGGAGGAG AGCGAGGGCT ACGCCAAGCT GACCACGGAG 
CTGAGCCAGG AGATCACCAG CAAGATCAGC CCCGATTACA TGATAGAAGT GATGCGCTCG 
CTCATTGGCT GCTTCAATCT GGACCCCAAC CGCGTGTTGG ACGTGGTTCT GGAGGCGTTT 
GAGTGCCGAC TGCATCTGGA GGACTTCTTT GTCCCTCTGC TGACAAAGTT CCTTCGCAAC 
CCTGCCACCA TAAGCCAGGT GCTGGGCTTC AAGTTCACCT TCTACCAGGT GAGGGGCGAA 
GCGGGAGAGC CCACCCCAGC GTCCCTGTAC CGGCTGGCCG CCCTCCTGAT CAGGAACGAG 
CTGATTGCCC TGGACGACCT CTACTCCCTG CTGCTGCCCG ACGACGGGGC CCTGTCGAGG 
CAGCACAAGA AGGAGGTGGC GGCGGCTAAG ACGTACGCCC GGAGAGCGAC CGTGGTGGTG 
GTCTCGTCGG ACAAGAAGGA CGAGGAGAAG AAGGAGGAGG ACAAGGACGA CGACCTGACC 
CTGGAGGAGA ACCAGAAGCT GGGCCTGTGT GAGGCCCTGC TGGAAGTGGG CGACTGGCCC 
AACGCCCAAA AGCTGATGGC GCGCCTGCCC GAGCACTACG CCGTGTCGCA GCCCCACATT 
GCCCGGCGCC TCTGCCTCCT GGTCCACACC CTGGTGGAGC CCTTGTACCG CAGGCACAGC 
GGGCTGCCGG AGGGCTTCCG GCGCTCGTAC GCGTCCTCCC GTCGGGGGGG CCTGCGGCCG 
CCCGTCCTCG TGGAGACCTT CGGGGACTTC CAGGCACGCG CCCTGCCCAT GCTCGTGGCC 
CTGGGCCCCA TGCTGCACCT GGACCCTGTG CTCATCGTCA AGGTGGTGCG CCTGGCCAGA 
GCGCACCTGC ACAAGACCAG CTCCTCGGCG GCTGGAGACC TGCGGTTCGA CTTGCTCACC 
CTGGTCGGGG AGAGCCTGCT GCCCTCCCTG TCCCTTCTCG ACTGCAACTG CTGCGTGGCC 
GACGAGATCT GGTCCCTGCT CAAGCTCTAC CCCTACCAGC AGAGGTACCG TCTGTACAGC 
CAGTGGAAGA ACGAGGCGTA CGTGAGTCAC CCGTTGCTCA TTCGGGTGAA GGCGGACTCG 
CTCAAGAGGA TCAAGTACAT CATGAAGAGA CTCAGCAAGG AAAATGTGAA GCCTTCGGGG 
CGACAGATTG GCAAGCTCAG CCACAGCAAC CCTTGCTTTC TCTTTGACTA TATCCTGTCC 
CAGATCCAGA CCTGGGACAA CCTGATCGTG CCCGTGGTGG ACTCCCTCAA GTACCTCACC 
ATGCTCTCCT ACGACGTTCT CGCCTACTGC GTGGCGGAGG CGCTCTGCAA TCCGGAGAAG 
GACCGCATGA AGCATGACGG CACCAGCATC TCCCTCTGGC TGCAGAGTCT GGCCAGTTTC 
TGTGGCGCCG TCTTCAAGAA GTACTCCATC GACCTGACGG GACTGCTGCA GCTCGTGGCA 
AATCAGCTGA AGGCAGAAAA AAGCCTTGAC CTGCTGGTGC TGAAGGACAT CGTACAGAAG 
ATGACCGGCA TCGAGTCGAC GGAACAGGCA ACGCAGGACC AACTGGAGGC GATGTGCGGA 
GGCGAACTGC TTAAGGCCGA GGGCGGCTAC TTCCACCAGC TGCGCAACAC CAAGAAGTCG 
TCCCAGCGGC TGAAGGAGGC CCTGCTGGAG CAGGACCTGG CCCTGCCGCT CTGCCTGCTC 
ATGGCCCAGC AGAAGAACTG CATCCTCTAC CGGGACCAGG AGGCCTCCCA CCTCAAGCTG 
GTTGGAAAGC TCTACGACCA GTGTCAGGAC ACCCTGGTCC AGTTTGGCAG CTTCCTGTCG 
AGCAGCCTGA GCACCGAAGA GTACGCCGGC CGCCTGCCGC CCGTGGACCA GCTACTCTCG 
CGGTTCCACG TGCAGGCCGA CGTGGCCTTC TTTTTGGCCA GGCCCATGTT CGGACATTCC 
ATCGCGCTCA AGTTCGACGA CGCCAAGAGG AGGGACAAGA CGTTCAAAAG TCTAACCGCC 
GCGCAGAAGT TGCAGCGCTA CGTCGACGCG GTGGACCAGG TGATGTCGCG GGTCGTCGAG 
AGTGTGCGGC CGCTGCACCC GTCCAAGACG TGGGAGGACC TCAGCCCGCA GTTCTACGTC 
ACCTTCTGGT CGCTCTCGAT GTACGACCTC CACGTGCCCA CGTCCAGCTA CGAGAGGGAG 
GTGGGCAAGT TGAAGCAGCA GGTGGTGCAG GCGGAGGACA GCAAGGACAT GGTGCCCAGC 
AAGCGCAAGA AGGAGCGCGA CCGGTGCGAA GCGCTCATGG AGAAGCTGCA GGAGGAGGAG 
CGCAAGCAGC AGGACCACTG CGGGCGCGTC CTGGCACGGC TGCGCAGCGA GAAGGATGCC 
TGGTTCCACT CCCGTTCGGC CAAGAACGAG ACCATCACGC AGTTCCTGCA GCTGTGCGTG 
TTCCCGCGCT GCACGTTCAC GGCCCTGGAC GCCCTCTACT GTGCCAAGTT TGTGCACCTG 
ATCCACATCC TGAAGACGCC AAACTTCTCC ACCCTCATCT GCTACGACAA GATCTTCTGC 
GACATCACTT ACACAGTGAC GGCATGCACG GAGAACGAAG CGAACCGCTA CGGGCGCTTC 
CTCTGCTCCA TGCTGGAGAC GGTGATGCGG TGGCACAGCG ACAAGGCCAT CTTTGACAAG 
GAGTGTGCCA ACTTCCCCGG CTTCATGACC AAGTTCCGGG GCGCCAGCCA GTCGTCGGAC 
ATTGCCACGG ACCACGTGGA CTACGAGAAC TACCGGCACG TCTGTCACAA GTGGCACTTC 
AAGATCACTA AGGCCCTGGT CATCTGTCTG GAGTCTGGGG ACTACGTCCA GATCCGGAAC 
AGCCTGATTG TGCTCACCAA GGTGCTGCCC CACTTCCCGG TCATGACCAA CTTGGGCCAG 
GCGCTGGAGC GGCGCATCGA GAAGATCCGC CAGGAGGAGA AGGAGAAGAG GCCGGACCTC 
TTTGCTCTTG CCACCGGCTA CGCCGGCCAG CTGAAGGGCA AGAAATCCGA GCTGATCCCG 
GAGCACGAGT TCCACCTCAA GGACAGCAAA GACGGCCGCG CGGTGAGCGC CACCGCACAG 
GTGAAGAGCG CTGCAGATCA CACGGTGACG ACGGCGGCAC ATGCCGCCAC CTCTTCCCAA 
GTGGCGAGTG TCGATGGTGC CAAGCAGAAG CGGGGAGACT CCGTGAGGGA GTCCAACTCG 
TCACCAGCAC TGTCCGGCAA TTCTTCTGGG AAGCTGTCGA GCAGCGGCGG AGGTCTCGTC 
CAGTCTTCGT CTTCGTCGTC GTCGTCCAAG ATGACATCGT CCAGTAGTTC TTCCGCCACC 
CGCAGGGAGT CCAACAGGTC GCTGAGCAAG GAGTCCGGGA GCGGGGACGG ACACGACAAG 
GGCGCCGCCA CCGACCGCAA GGGCAGCTCG CTGTCCTCCC TCCACGAGCA GGACGTCAGG 
CCCTCCAAGG ACGACGTCCA CAAAAATGAC CACTGGCTGG AGCCCGGGGA CGCGCCCGGA 
GACCACAAGC GGCGGCGCGT GGACGGGGGC AGCAGCAGCG GCGGCAGCAG CAGTGGCAAG 
GTGGGAGGCA GTCCCCGGGG CCGTAGCCTC GGGGGCACGC AGAGCCCCAA GGTCTCGGAA 
CCCGAGGGGA AGTCTGAGGA CCGCGGGGGA GGGGGGGACC GCCCTTCTGA GAAGTGGGCT 
CACCCCAAGG ACTCCCACGA CCCGGCCACG GGGGACAAGG GCAAGGCCAA GAAGGGCAGC 
CGCAAGCGGG ACCACTCCGA CGAGCCCCCG CTCGAGGGCA AGCGCAAGAA GGACGAGGAC 
GCCTTGAAGA GCTCGAGCAA GCGCAACGGG GAGGACTCTA AGGAGAAGGA GCGGTACCGG 
AGGGTGAGAG AGCAGCAGCC CCTGCTGCTG GGGCTCCCTC GTGACCAGCT GGTCGCACTC 
TCTCCTCAGG AAGAGGACAA GCTGCGAGAG TCCCCCAAGC TGCGAGAGCG CAGGGCCGAC 
CGGGACGACA AGAAGCATCA TCGATCGAGC AGCAGCAGTG GCAGCTCGAC GTCAACGAAA 
AAGCGATCAT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029843671.1
CDS	13..4590
Translation	MAGRSVPLDICKSWERTGKSDFVKLCRSAASCMQTSRLPFDKGGKEMQLLLHDLCWHVLEDRLKVEQAVSALAEVTALHQELPSMLADVIFLLDMETLSVENRDQRDRFYWLVGGCAKVVPDSLLKERLDIETLGDGGIVKNSRVMLTKFVKIKTRLFYKQNKYNLFREESEGYAKLTTELSQEITSKISPDYMIEVMRSLIGCFNLDPNRVLDVVLEAFECRLHLEDFFVPLLTKFLRNPATISQVLGFKFTFYQVRGEAGEPTPASLYRLAALLIRNELIALDDLYSLLLPDDGALSRQHKKEVAAAKTYARRATVVVVSSDKKDEEKKEEDKDDDLTLEENQKLGLCEALLEVGDWPNAQKLMARLPEHYAVSQPHIARRLCLLVHTLVEPLYRRHSGLPEGFRRSYASSRRGGLRPPVLVETFGDFQARALPMLVALGPMLHLDPVLIVKVVRLARAHLHKTSSSAAGDLRFDLLTLVGESLLPSLSLLDCNCCVADEIWSLLKLYPYQQRYRLYSQWKNEAYVSHPLLIRVKADSLKRIKYIMKRLSKENVKPSGRQIGKLSHSNPCFLFDYILSQIQTWDNLIVPVVDSLKYLTMLSYDVLAYCVAEALCNPEKDRMKHDGTSISLWLQSLASFCGAVFKKYSIDLTGLLQLVANQLKAEKSLDLLVLKDIVQKMTGIESTEQATQDQLEAMCGGELLKAEGGYFHQLRNTKKSSQRLKEALLEQDLALPLCLLMAQQKNCILYRDQEASHLKLVGKLYDQCQDTLVQFGSFLSSSLSTEEYAGRLPPVDQLLSRFHVQADVAFFLARPMFGHSIALKFDDAKRRDKTFKSLTAAQKLQRYVDAVDQVMSRVVESVRPLHPSKTWEDLSPQFYVTFWSLSMYDLHVPTSSYEREVGKLKQQVVQAEDSKDMVPSKRKKERDRCEALMEKLQEEERKQQDHCGRVLARLRSEKDAWFHSRSAKNETITQFLQLCVFPRCTFTALDALYCAKFVHLIHILKTPNFSTLICYDKIFCDITYTVTACTENEANRYGRFLCSMLETVMRWHSDKAIFDKECANFPGFMTKFRGASQSSDIATDHVDYENYRHVCHKWHFKITKALVICLESGDYVQIRNSLIVLTKVLPHFPVMTNLGQALERRIEKIRQEEKEKRPDLFALATGYAGQLKGKKSELIPEHEFHLKDSKDGRAVSATAQVKSAADHTVTTAAHAATSSQVASVDGAKQKRGDSVRESNSSPALSGNSSGKLSSSGGGLVQSSSSSSSSKMTSSSSSSATRRESNRSLSKESGSGDGHDKGAATDRKGSSLSSLHEQDVRPSKDDVHKNDHWLEPGDAPGDHKRRRVDGGSSSGGSSSGKVGGSPRGRSLGGTQSPKVSEPEGKSEDRGGGGDRPSEKWAHPKDSHDPATGDKGKAKKGSRKRDHSDEPPLEGKRKKDEDALKSSSKRNGEDSKEKERYRRVREQQPLLLGLPRDQLVALSPQEEDKLRESPKLRERRADRDDKKHHRSSSSSGSSTSTKKRSSF

Target ORF information:

RefSeq Version	XM_029987811.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029987811.1

ORF Insert Sequence:

ATGGCGGGCC GGTCTGTACC GTTGGACATT TGTAAAAGTT GGGAAAGGAC GGGGAAATCG 
GACTTTGTCA AACTATGTCG CTCAGCGGCA TCATGCATGC AGACGTCGAG GCTGCCTTTC 
GACAAGGGTG GAAAAGAGAT GCAGCTTCTG TTACACGACC TCTGCTGGCA CGTGCTGGAG 
GATCGCCTGA AAGTTGAGCA AGCTGTGTCG GCGCTGGCCG AAGTGACTGC CTTGCATCAG 
GAGCTGCCCT CGATGCTGGC AGACGTCATC TTTCTGCTGG ACATGGAGAC GCTGTCGGTG 
GAGAACCGTG ACCAGAGGGA CCGTTTTTAC TGGCTCGTCG GCGGCTGTGC AAAGGTTGTT 
CCCGACTCGC TGCTGAAGGA GCGGCTGGAC ATCGAGACCT TGGGAGACGG CGGGATCGTG 
AAGAACAGCC GTGTCATGCT CACCAAGTTT GTCAAGATCA AGACACGACT GTTCTACAAG 
CAGAACAAGT ACAACCTGTT CCGGGAGGAG AGCGAGGGCT ACGCCAAGCT GACCACGGAG 
CTGAGCCAGG AGATCACCAG CAAGATCAGC CCCGATTACA TGATAGAAGT GATGCGCTCG 
CTCATTGGCT GCTTCAATCT GGACCCCAAC CGCGTGTTGG ACGTGGTTCT GGAGGCGTTT 
GAGTGCCGAC TGCATCTGGA GGACTTCTTT GTCCCTCTGC TGACAAAGTT CCTTCGCAAC 
CCTGCCACCA TAAGCCAGGT GCTGGGCTTC AAGTTCACCT TCTACCAGGT GAGGGGCGAA 
GCGGGAGAGC CCACCCCAGC GTCCCTGTAC CGGCTGGCCG CCCTCCTGAT CAGGAACGAG 
CTGATTGCCC TGGACGACCT CTACTCCCTG CTGCTGCCCG ACGACGGGGC CCTGTCGAGG 
CAGCACAAGA AGGAGGTGGC GGCGGCTAAG ACGTACGCCC GGAGAGCGAC CGTGGTGGTG 
GTCTCGTCGG ACAAGAAGGA CGAGGAGAAG AAGGAGGAGG ACAAGGACGA CGACCTGACC 
CTGGAGGAGA ACCAGAAGCT GGGCCTGTGT GAGGCCCTGC TGGAAGTGGG CGACTGGCCC 
AACGCCCAAA AGCTGATGGC GCGCCTGCCC GAGCACTACG CCGTGTCGCA GCCCCACATT 
GCCCGGCGCC TCTGCCTCCT GGTCCACACC CTGGTGGAGC CCTTGTACCG CAGGCACAGC 
GGGCTGCCGG AGGGCTTCCG GCGCTCGTAC GCGTCCTCCC GTCGGGGGGG CCTGCGGCCG 
CCCGTCCTCG TGGAGACCTT CGGGGACTTC CAGGCACGCG CCCTGCCCAT GCTCGTGGCC 
CTGGGCCCCA TGCTGCACCT GGACCCTGTG CTCATCGTCA AGGTGGTGCG CCTGGCCAGA 
GCGCACCTGC ACAAGACCAG CTCCTCGGCG GCTGGAGACC TGCGGTTCGA CTTGCTCACC 
CTGGTCGGGG AGAGCCTGCT GCCCTCCCTG TCCCTTCTCG ACTGCAACTG CTGCGTGGCC 
GACGAGATCT GGTCCCTGCT CAAGCTCTAC CCCTACCAGC AGAGGTACCG TCTGTACAGC 
CAGTGGAAGA ACGAGGCGTA CGTGAGTCAC CCGTTGCTCA TTCGGGTGAA GGCGGACTCG 
CTCAAGAGGA TCAAGTACAT CATGAAGAGA CTCAGCAAGG AAAATGTGAA GCCTTCGGGG 
CGACAGATTG GCAAGCTCAG CCACAGCAAC CCTTGCTTTC TCTTTGACTA TATCCTGTCC 
CAGATCCAGA CCTGGGACAA CCTGATCGTG CCCGTGGTGG ACTCCCTCAA GTACCTCACC 
ATGCTCTCCT ACGACGTTCT CGCCTACTGC GTGGCGGAGG CGCTCTGCAA TCCGGAGAAG 
GACCGCATGA AGCATGACGG CACCAGCATC TCCCTCTGGC TGCAGAGTCT GGCCAGTTTC 
TGTGGCGCCG TCTTCAAGAA GTACTCCATC GACCTGACGG GACTGCTGCA GCTCGTGGCA 
AATCAGCTGA AGGCAGAAAA AAGCCTTGAC CTGCTGGTGC TGAAGGACAT CGTACAGAAG 
ATGACCGGCA TCGAGTCGAC GGAACAGGCA ACGCAGGACC AACTGGAGGC GATGTGCGGA 
GGCGAACTGC TTAAGGCCGA GGGCGGCTAC TTCCACCAGC TGCGCAACAC CAAGAAGTCG 
TCCCAGCGGC TGAAGGAGGC CCTGCTGGAG CAGGACCTGG CCCTGCCGCT CTGCCTGCTC 
ATGGCCCAGC AGAAGAACTG CATCCTCTAC CGGGACCAGG AGGCCTCCCA CCTCAAGCTG 
GTTGGAAAGC TCTACGACCA GTGTCAGGAC ACCCTGGTCC AGTTTGGCAG CTTCCTGTCG 
AGCAGCCTGA GCACCGAAGA GTACGCCGGC CGCCTGCCGC CCGTGGACCA GCTACTCTCG 
CGGTTCCACG TGCAGGCCGA CGTGGCCTTC TTTTTGGCCA GGCCCATGTT CGGACATTCC 
ATCGCGCTCA AGTTCGACGA CGCCAAGAGG AGGGACAAGA CGTTCAAAAG TCTAACCGCC 
GCGCAGAAGT TGCAGCGCTA CGTCGACGCG GTGGACCAGG TGATGTCGCG GGTCGTCGAG 
AGTGTGCGGC CGCTGCACCC GTCCAAGACG TGGGAGGACC TCAGCCCGCA GTTCTACGTC 
ACCTTCTGGT CGCTCTCGAT GTACGACCTC CACGTGCCCA CGTCCAGCTA CGAGAGGGAG 
GTGGGCAAGT TGAAGCAGCA GGTGGTGCAG GCGGAGGACA GCAAGGACAT GGTGCCCAGC 
AAGCGCAAGA AGGAGCGCGA CCGGTGCGAA GCGCTCATGG AGAAGCTGCA GGAGGAGGAG 
CGCAAGCAGC AGGACCACTG CGGGCGCGTC CTGGCACGGC TGCGCAGCGA GAAGGATGCC 
TGGTTCCACT CCCGTTCGGC CAAGAACGAG ACCATCACGC AGTTCCTGCA GCTGTGCGTG 
TTCCCGCGCT GCACGTTCAC GGCCCTGGAC GCCCTCTACT GTGCCAAGTT TGTGCACCTG 
ATCCACATCC TGAAGACGCC AAACTTCTCC ACCCTCATCT GCTACGACAA GATCTTCTGC 
GACATCACTT ACACAGTGAC GGCATGCACG GAGAACGAAG CGAACCGCTA CGGGCGCTTC 
CTCTGCTCCA TGCTGGAGAC GGTGATGCGG TGGCACAGCG ACAAGGCCAT CTTTGACAAG 
GAGTGTGCCA ACTTCCCCGG CTTCATGACC AAGTTCCGGG GCGCCAGCCA GTCGTCGGAC 
ATTGCCACGG ACCACGTGGA CTACGAGAAC TACCGGCACG TCTGTCACAA GTGGCACTTC 
AAGATCACTA AGGCCCTGGT CATCTGTCTG GAGTCTGGGG ACTACGTCCA GATCCGGAAC 
AGCCTGATTG TGCTCACCAA GGTGCTGCCC CACTTCCCGG TCATGACCAA CTTGGGCCAG 
GCGCTGGAGC GGCGCATCGA GAAGATCCGC CAGGAGGAGA AGGAGAAGAG GCCGGACCTC 
TTTGCTCTTG CCACCGGCTA CGCCGGCCAG CTGAAGGGCA AGAAATCCGA GCTGATCCCG 
GAGCACGAGT TCCACCTCAA GGACAGCAAA GACGGCCGCG CGGTGAGCGC CACCGCACAG 
GTGAAGAGCG CTGCAGATCA CACGGTGACG ACGGCGGCAC ATGCCGCCAC CTCTTCCCAA 
GTGGCGAGTG TCGATGGTGC CAAGCAGAAG CGGGGAGACT CCGTGAGGGA GTCCAACTCG 
TCACCAGCAC TGTCCGGCAA TTCTTCTGGG AAGCTGTCGA GCAGCGGCGG AGGTCTCGTC 
CAGTCTTCGT CTTCGTCGTC GTCGTCCAAG ATGACATCGT CCAGTAGTTC TTCCGCCACC 
CGCAGGGAGT CCAACAGGTC GCTGAGCAAG GAGTCCGGGA GCGGGGACGG ACACGACAAG 
GGCGCCGCCA CCGACCGCAA GGGCAGCTCG CTGTCCTCCC TCCACGAGCA GGACGTCAGG 
CCCTCCAAGG ACGACGTCCA CAAAAATGAC CACTGGCTGG AGCCCGGGGA CGCGCCCGGA 
GACCACAAGC GGCGGCGCGT GGACGGGGGC AGCAGCAGCG GCGGCAGCAG CAGTGGCAAG 
GTGGGAGGCA GTCCCCGGGG CCGTAGCCTC GGGGGCACGC AGAGCCCCAA GGTCTCGGAA 
CCCGAGGGGA AGTCTGAGGA CCGCGGGGGA GGGGGGGACC GCCCTTCTGA GAAGTGGGCT 
CACCCCAAGG ACTCCCACGA CCCGGCCACG GGGGACAAGG GCAAGGCCAA GAAGGGCAGC 
CGCAAGCGGG ACCACTCCGA CGAGCCCCCG CTCGAGGGCA AGCGCAAGAA GGACGAGGAC 
GCCTTGAAGA GCTCGAGCAA GCGCAACGGG GAGGACTCTA AGGAGAAGGA GCGGTACCGG 
AGGGTGAGAG AGCAGCAGCC CCTGCTGCTG GGGCTCCCTC GTGACCAGCT GGTCGCACTC 
TCTCCTCAGG AAGAGGACAA GCTGCGAGAG TCCCCCAAGC TGCGAGAGCG CAGGGCCGAC 
CGGGACGACA AGAAGCATCA TCGATCGAGC AGCAGCAGTG GCAGCTCGAC GTCAACGAAA 
AAGCGATCAT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8029916 ( XM_029987812.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029843672.1 Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OIa29770
Clone ID Related Accession (Same CDS sequence)	XM_029987812.1
Accession Version	XM_029987812.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	THO complex subunit 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021921043.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGCC GGTCTGTACC GTTGGACATT TGTAAAAGTT GGGAAAGGAC GGGGAAATCG 
GACTTTGTCA AACTATGTCG CTCAGCGGCA TCATGCATGC AGACGTCGAG GCTGCCTTTC 
GACAAGGGTG GAAAAGAGAT GCAGCTTCTG TTACACGACC TCTGCTGGCA CGTGCTGGAG 
GATCGCCTGA AAGTTGAGCA AGCTGTGTCG GCGCTGGCCG AAGTGACTGC CTTGCATCAG 
GAGCTGCCCT CGATGCTGGC AGACGTCATC TTTCTGCTGG ACATGGAGAC GCTGTCGGTG 
GAGAACCGTG ACCAGAGGGA CCGTTTTTAC TGGCTCGTCG GCGGCTGTGC AAAGGTTGTT 
CCCGACTCGC TGCTGAAGGA GCGGCTGGAC ATCGAGACCT TGGGAGACGG CGGGATCGTG 
AAGAACAGCC GTGTCATGCT CACCAAGTTT GTCAAGATCA AGACACGACT GTTCTACAAG 
CAGAACAAGT ACAACCTGTT CCGGGAGGAG AGCGAGGGCT ACGCCAAGCT GACCACGGAG 
CTGAGCCAGG AGATCACCAG CAAGATCAGC CCCGATTACA TGATAGAAGT GATGCGCTCG 
CTCATTGGCT GCTTCAATCT GGACCCCAAC CGCGTGTTGG ACGTGGTTCT GGAGGCGTTT 
GAGTGCCGAC TGCATCTGGA GGACTTCTTT GTCCCTCTGC TGACAAAGTT CCTTCGCAAC 
CCTGCCACCA TAAGCCAGGT GCTGGGCTTC AAGTTCACCT TCTACCAGGT GAGGGGCGAA 
GCGGGAGAGC CCACCCCAGC GTCCCTGTAC CGGCTGGCCG CCCTCCTGAT CAGGAACGAG 
CTGATTGCCC TGGACGACCT CTACTCCCTG CTGCTGCCCG ACGACGGGGC CCTGTCGAGG 
CAGCACAAGA AGGAGGTGGC GGCGGCTAAG ACGTACGCCC GGAGAGCGAC CGTGGTGGTG 
GTCTCGTCGG ACAAGAAGGA CGAGGAGAAG AAGGAGGAGG ACAAGGACGA CGACCTGACC 
CTGGAGGAGA ACCAGAAGCT GGGCCTGTGT GAGGCCCTGC TGGAAGTGGG CGACTGGCCC 
AACGCCCAAA AGCTGATGGC GCGCCTGCCC GAGCACTACG CCGTGTCGCA GCCCCACATT 
GCCCGGCGCC TCTGCCTCCT GGTCCACACC CTGGTGGAGC CCTTGTACCG CAGGCACAGC 
GGGCTGCCGG AGGGCTTCCG GCGCTCGTAC GCGTCCTCCC GTCGGGGGGG CCTGCGGCCG 
CCCGTCCTCG TGGAGACCTT CGGGGACTTC CAGGCACGCG CCCTGCCCAT GCTCGTGGCC 
CTGGGCCCCA TGCTGCACCT GGACCCTGTG CTCATCGTCA AGGTGGTGCG CCTGGCCAGA 
GCGCACCTGC ACAAGACCAG CTCCTCGGCG GCTGGAGACC TGCGGTTCGA CTTGCTCACC 
CTGGTCGGGG AGAGCCTGCT GCCCTCCCTG TCCCTTCTCG ACTGCAACTG CTGCGTGGCC 
GACGAGATCT GGTCCCTGCT CAAGCTCTAC CCCTACCAGC AGAGGTACCG TCTGTACAGC 
CAGTGGAAGA ACGAGGCGTA CGTGAGTCAC CCGTTGCTCA TTCGGGTGAA GGCGGACTCG 
CTCAAGAGGA TCAAGTACAT CATGAAGAGA CTCAGCAAGG AAAATGTGAA GCCTTCGGGG 
CGACAGATTG GCAAGCTCAG CCACAGCAAC CCTTGCTTTC TCTTTGACTA TATCCTGTCC 
CAGATCCAGA CCTGGGACAA CCTGATCGTG CCCGTGGTGG ACTCCCTCAA GTACCTCACC 
ATGCTCTCCT ACGACGTTCT CGCCTACTGC GTGGCGGAGG CGCTCTGCAA TCCGGAGAAG 
GACCGCATGA AGCATGACGG CACCAGCATC TCCCTCTGGC TGCAGAGTCT GGCCAGTTTC 
TGTGGCGCCG TCTTCAAGAA GTACTCCATC GACCTGACGG GACTGCTGCA GCTCGTGGCA 
AATCAGCTGA AGGCAGAAAA AAGCCTTGAC CTGCTGGTGC TGAAGGACAT CGTACAGAAG 
ATGACCGGCA TCGAGTCGAC GGAACAGGCA ACGCAGGACC AACTGGAGGC GATGTGCGGA 
GGCGAACTGC TTAAGGCCGA GGGCGGCTAC TTCCACCAGC TGCGCAACAC CAAGAAGTCG 
TCCCAGCGGC TGAAGGAGGC CCTGCTGGAG CAGGACCTGG CCCTGCCGCT CTGCCTGCTC 
ATGGCCCAGC AGAAGAACTG CATCCTCTAC CGGGACCAGG AGGCCTCCCA CCTCAAGCTG 
GTTGGAAAGC TCTACGACCA GTGTCAGGAC ACCCTGGTCC AGTTTGGCAG CTTCCTGTCG 
AGCAGCCTGA GCACCGAAGA GTACGCCGGC CGCCTGCCGC CCGTGGACCA GCTACTCTCG 
CGGTTCCACG TGCAGGCCGA CGTGGCCTTC TTTTTGGCCA GGCCCATGTT CGGACATTCC 
ATCGCGCTCA AGTTCGACGA CGCCAAGAGG AGGGACAAGA CGTTCAAAAG TCTAACCGCC 
GCGCAGAAGT TGCAGCGCTA CGTCGACGCG GTGGACCAGG TGATGTCGCG GGTCGTCGAG 
AGTGTGCGGC CGCTGCACCC GTCCAAGACG TGGGAGGACC TCAGCCCGCA GTTCTACGTC 
ACCTTCTGGT CGCTCTCGAT GTACGACCTC CACGTGCCCA CGTCCAGCTA CGAGAGGGAG 
GTGGGCAAGT TGAAGCAGCA GGTGGTGCAG GCGGAGGACA GCAAGGACAT GGTGCCCAGC 
AAGCGCAAGA AGGAGCGCGA CCGGTGCGAA GCGCTCATGG AGAAGCTGCA GGAGGAGGAG 
CGCAAGCAGC AGGACCACTG CGGGCGCGTC CTGGCACGGC TGCGCAGCGA GAAGGATGCC 
TGGTTCCACT CCCGTTCGGC CAAGAACGAG ACCATCACGC AGTTCCTGCA GCTGTGCGTG 
TTCCCGCGCT GCACGTTCAC GGCCCTGGAC GCCCTCTACT GTGCCAAGTT TGTGCACCTG 
ATCCACATCC TGAAGACGCC AAACTTCTCC ACCCTCATCT GCTACGACAA GATCTTCTGC 
GACATCACTT ACACAGTGAC GGCATGCACG GAGAACGAAG CGAACCGCTA CGGGCGCTTC 
CTCTGCTCCA TGCTGGAGAC GGTGATGCGG TGGCACAGCG ACAAGGCCAT CTTTGACAAG 
GAGTGTGCCA ACTTCCCCGG CTTCATGACC AAGTTCCGGG GCGCCAGCCA GTCGTCGGAC 
ATTGCCACGG ACCACGTGGA CTACGAGAAC TACCGGCACG TCTGTCACAA GTGGCACTTC 
AAGATCACTA AGGCCCTGGT CATCTGTCTG GAGTCTGGGG ACTACGTCCA GATCCGGAAC 
AGCCTGATTG TGCTCACCAA GGTGCTGCCC CACTTCCCGG TCATGACCAA CTTGGGCCAG 
GCGCTGGAGC GGCGCATCGA GAAGATCCGC CAGGAGGAGA AGGAGAAGAG GCCGGACCTC 
TTTGCTCTTG CCACCGGCTA CGCCGGCCAG CTGAAGGGCA AGAAATCCGA GCTGATCCCG 
GAGCACGAGT TCCACCTCAA GGACAGCAAA GACGGCCGCG CGGTGAGCGC CACCGCACAG 
GTGAAGAGCG CTGCAGATCA CACGGTGACG ACGGCGGCAC ATGCCGCCAC CTCTTCCCAA 
GTGGCGAGTG TCGATGGTGC CAAGCAGAAG CGGGGAGACT CCGTGAGGGA GTCCAACTCG 
TCACCAGCAC TGTCCGGCAA TTCTTCTGGG AAGCTGTCGA GCAGCGGCGG AGGTCTCGTC 
CAGTCTTCGT CTTCGTCGTC GTCGTCCAAG ATGACATCGT CCAGTAGTTC TTCCGCCACC 
CGCAGGGAGT CCAACAGCTT TGGTGCGGTT GTGGAGAGTG CCACGACGGC CGCTTTTCCT 
CGGAGGTCGC TGAGCAAGGA GTCCGGGAGC GGGGACGGAC ACGACAAGGG CGCCGCCACC 
GACCGCAAGG GCAGCTCGCT GTCCTCCCTC CACGAGCAGG ACGTCAGGCC CTCCAAGGAC 
GACGTCCACA AAAATGACCA CTGGCTGGAG CCCGGGGACG CGCCCGGAGA CCACAAGCGG 
CGGCGCGTGG ACGGGGGCAG CAGCAGCGGC GGCAGCAGCA GTGGCAAGGT GGGAGGCAGT 
CCCCGGGGCC GTAGCCTCGG GGGCACGCAG AGCCCCAAGG TCTCGGAACC CGAGGGGAAG 
TCTGAGGACC GCGGGGGAGG GGGGGACCGC CCTTCTGAGA AGTGGGCTCA CCCCAAGGAC 
TCCCACGACC CGGCCACGGG GGACAAGGGC AAGGCCAAGA AGGGCAGCCG CAAGCGGGAC 
CACTCCGACG AGCCCCCGCT CGAGGGCAAG CGCAAGAAGG ACGAGGACGC CTTGAAGAGC 
TCGAGCAAGC GCAACGGGGA GGACTCTAAG GAGAAGGAGC GGTACCGGAG GGAAGAGGAC 
AAGCTGCGAG AGTCCCCCAA GCTGCGAGAG CGCAGGGCCG ACCGGGACGA CAAGAAGCAT 
CATCGATCGA GCAGCAGCAG TGGCAGCTCG ACGTCAACGA AAAAGCGATC ATCCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029843672.1
CDS	13..4572
Translation	MAGRSVPLDICKSWERTGKSDFVKLCRSAASCMQTSRLPFDKGGKEMQLLLHDLCWHVLEDRLKVEQAVSALAEVTALHQELPSMLADVIFLLDMETLSVENRDQRDRFYWLVGGCAKVVPDSLLKERLDIETLGDGGIVKNSRVMLTKFVKIKTRLFYKQNKYNLFREESEGYAKLTTELSQEITSKISPDYMIEVMRSLIGCFNLDPNRVLDVVLEAFECRLHLEDFFVPLLTKFLRNPATISQVLGFKFTFYQVRGEAGEPTPASLYRLAALLIRNELIALDDLYSLLLPDDGALSRQHKKEVAAAKTYARRATVVVVSSDKKDEEKKEEDKDDDLTLEENQKLGLCEALLEVGDWPNAQKLMARLPEHYAVSQPHIARRLCLLVHTLVEPLYRRHSGLPEGFRRSYASSRRGGLRPPVLVETFGDFQARALPMLVALGPMLHLDPVLIVKVVRLARAHLHKTSSSAAGDLRFDLLTLVGESLLPSLSLLDCNCCVADEIWSLLKLYPYQQRYRLYSQWKNEAYVSHPLLIRVKADSLKRIKYIMKRLSKENVKPSGRQIGKLSHSNPCFLFDYILSQIQTWDNLIVPVVDSLKYLTMLSYDVLAYCVAEALCNPEKDRMKHDGTSISLWLQSLASFCGAVFKKYSIDLTGLLQLVANQLKAEKSLDLLVLKDIVQKMTGIESTEQATQDQLEAMCGGELLKAEGGYFHQLRNTKKSSQRLKEALLEQDLALPLCLLMAQQKNCILYRDQEASHLKLVGKLYDQCQDTLVQFGSFLSSSLSTEEYAGRLPPVDQLLSRFHVQADVAFFLARPMFGHSIALKFDDAKRRDKTFKSLTAAQKLQRYVDAVDQVMSRVVESVRPLHPSKTWEDLSPQFYVTFWSLSMYDLHVPTSSYEREVGKLKQQVVQAEDSKDMVPSKRKKERDRCEALMEKLQEEERKQQDHCGRVLARLRSEKDAWFHSRSAKNETITQFLQLCVFPRCTFTALDALYCAKFVHLIHILKTPNFSTLICYDKIFCDITYTVTACTENEANRYGRFLCSMLETVMRWHSDKAIFDKECANFPGFMTKFRGASQSSDIATDHVDYENYRHVCHKWHFKITKALVICLESGDYVQIRNSLIVLTKVLPHFPVMTNLGQALERRIEKIRQEEKEKRPDLFALATGYAGQLKGKKSELIPEHEFHLKDSKDGRAVSATAQVKSAADHTVTTAAHAATSSQVASVDGAKQKRGDSVRESNSSPALSGNSSGKLSSSGGGLVQSSSSSSSSKMTSSSSSSATRRESNSFGAVVESATTAAFPRRSLSKESGSGDGHDKGAATDRKGSSLSSLHEQDVRPSKDDVHKNDHWLEPGDAPGDHKRRRVDGGSSSGGSSSGKVGGSPRGRSLGGTQSPKVSEPEGKSEDRGGGGDRPSEKWAHPKDSHDPATGDKGKAKKGSRKRDHSDEPPLEGKRKKDEDALKSSSKRNGEDSKEKERYRREEDKLRESPKLRERRADRDDKKHHRSSSSSGSSTSTKKRSSF

Target ORF information:

RefSeq Version	XM_029987812.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis THO complex subunit 2 (LOC8029916), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029987812.1

ORF Insert Sequence:

ATGGCGGGCC GGTCTGTACC GTTGGACATT TGTAAAAGTT GGGAAAGGAC GGGGAAATCG 
GACTTTGTCA AACTATGTCG CTCAGCGGCA TCATGCATGC AGACGTCGAG GCTGCCTTTC 
GACAAGGGTG GAAAAGAGAT GCAGCTTCTG TTACACGACC TCTGCTGGCA CGTGCTGGAG 
GATCGCCTGA AAGTTGAGCA AGCTGTGTCG GCGCTGGCCG AAGTGACTGC CTTGCATCAG 
GAGCTGCCCT CGATGCTGGC AGACGTCATC TTTCTGCTGG ACATGGAGAC GCTGTCGGTG 
GAGAACCGTG ACCAGAGGGA CCGTTTTTAC TGGCTCGTCG GCGGCTGTGC AAAGGTTGTT 
CCCGACTCGC TGCTGAAGGA GCGGCTGGAC ATCGAGACCT TGGGAGACGG CGGGATCGTG 
AAGAACAGCC GTGTCATGCT CACCAAGTTT GTCAAGATCA AGACACGACT GTTCTACAAG 
CAGAACAAGT ACAACCTGTT CCGGGAGGAG AGCGAGGGCT ACGCCAAGCT GACCACGGAG 
CTGAGCCAGG AGATCACCAG CAAGATCAGC CCCGATTACA TGATAGAAGT GATGCGCTCG 
CTCATTGGCT GCTTCAATCT GGACCCCAAC CGCGTGTTGG ACGTGGTTCT GGAGGCGTTT 
GAGTGCCGAC TGCATCTGGA GGACTTCTTT GTCCCTCTGC TGACAAAGTT CCTTCGCAAC 
CCTGCCACCA TAAGCCAGGT GCTGGGCTTC AAGTTCACCT TCTACCAGGT GAGGGGCGAA 
GCGGGAGAGC CCACCCCAGC GTCCCTGTAC CGGCTGGCCG CCCTCCTGAT CAGGAACGAG 
CTGATTGCCC TGGACGACCT CTACTCCCTG CTGCTGCCCG ACGACGGGGC CCTGTCGAGG 
CAGCACAAGA AGGAGGTGGC GGCGGCTAAG ACGTACGCCC GGAGAGCGAC CGTGGTGGTG 
GTCTCGTCGG ACAAGAAGGA CGAGGAGAAG AAGGAGGAGG ACAAGGACGA CGACCTGACC 
CTGGAGGAGA ACCAGAAGCT GGGCCTGTGT GAGGCCCTGC TGGAAGTGGG CGACTGGCCC 
AACGCCCAAA AGCTGATGGC GCGCCTGCCC GAGCACTACG CCGTGTCGCA GCCCCACATT 
GCCCGGCGCC TCTGCCTCCT GGTCCACACC CTGGTGGAGC CCTTGTACCG CAGGCACAGC 
GGGCTGCCGG AGGGCTTCCG GCGCTCGTAC GCGTCCTCCC GTCGGGGGGG CCTGCGGCCG 
CCCGTCCTCG TGGAGACCTT CGGGGACTTC CAGGCACGCG CCCTGCCCAT GCTCGTGGCC 
CTGGGCCCCA TGCTGCACCT GGACCCTGTG CTCATCGTCA AGGTGGTGCG CCTGGCCAGA 
GCGCACCTGC ACAAGACCAG CTCCTCGGCG GCTGGAGACC TGCGGTTCGA CTTGCTCACC 
CTGGTCGGGG AGAGCCTGCT GCCCTCCCTG TCCCTTCTCG ACTGCAACTG CTGCGTGGCC 
GACGAGATCT GGTCCCTGCT CAAGCTCTAC CCCTACCAGC AGAGGTACCG TCTGTACAGC 
CAGTGGAAGA ACGAGGCGTA CGTGAGTCAC CCGTTGCTCA TTCGGGTGAA GGCGGACTCG 
CTCAAGAGGA TCAAGTACAT CATGAAGAGA CTCAGCAAGG AAAATGTGAA GCCTTCGGGG 
CGACAGATTG GCAAGCTCAG CCACAGCAAC CCTTGCTTTC TCTTTGACTA TATCCTGTCC 
CAGATCCAGA CCTGGGACAA CCTGATCGTG CCCGTGGTGG ACTCCCTCAA GTACCTCACC 
ATGCTCTCCT ACGACGTTCT CGCCTACTGC GTGGCGGAGG CGCTCTGCAA TCCGGAGAAG 
GACCGCATGA AGCATGACGG CACCAGCATC TCCCTCTGGC TGCAGAGTCT GGCCAGTTTC 
TGTGGCGCCG TCTTCAAGAA GTACTCCATC GACCTGACGG GACTGCTGCA GCTCGTGGCA 
AATCAGCTGA AGGCAGAAAA AAGCCTTGAC CTGCTGGTGC TGAAGGACAT CGTACAGAAG 
ATGACCGGCA TCGAGTCGAC GGAACAGGCA ACGCAGGACC AACTGGAGGC GATGTGCGGA 
GGCGAACTGC TTAAGGCCGA GGGCGGCTAC TTCCACCAGC TGCGCAACAC CAAGAAGTCG 
TCCCAGCGGC TGAAGGAGGC CCTGCTGGAG CAGGACCTGG CCCTGCCGCT CTGCCTGCTC 
ATGGCCCAGC AGAAGAACTG CATCCTCTAC CGGGACCAGG AGGCCTCCCA CCTCAAGCTG 
GTTGGAAAGC TCTACGACCA GTGTCAGGAC ACCCTGGTCC AGTTTGGCAG CTTCCTGTCG 
AGCAGCCTGA GCACCGAAGA GTACGCCGGC CGCCTGCCGC CCGTGGACCA GCTACTCTCG 
CGGTTCCACG TGCAGGCCGA CGTGGCCTTC TTTTTGGCCA GGCCCATGTT CGGACATTCC 
ATCGCGCTCA AGTTCGACGA CGCCAAGAGG AGGGACAAGA CGTTCAAAAG TCTAACCGCC 
GCGCAGAAGT TGCAGCGCTA CGTCGACGCG GTGGACCAGG TGATGTCGCG GGTCGTCGAG 
AGTGTGCGGC CGCTGCACCC GTCCAAGACG TGGGAGGACC TCAGCCCGCA GTTCTACGTC 
ACCTTCTGGT CGCTCTCGAT GTACGACCTC CACGTGCCCA CGTCCAGCTA CGAGAGGGAG 
GTGGGCAAGT TGAAGCAGCA GGTGGTGCAG GCGGAGGACA GCAAGGACAT GGTGCCCAGC 
AAGCGCAAGA AGGAGCGCGA CCGGTGCGAA GCGCTCATGG AGAAGCTGCA GGAGGAGGAG 
CGCAAGCAGC AGGACCACTG CGGGCGCGTC CTGGCACGGC TGCGCAGCGA GAAGGATGCC 
TGGTTCCACT CCCGTTCGGC CAAGAACGAG ACCATCACGC AGTTCCTGCA GCTGTGCGTG 
TTCCCGCGCT GCACGTTCAC GGCCCTGGAC GCCCTCTACT GTGCCAAGTT TGTGCACCTG 
ATCCACATCC TGAAGACGCC AAACTTCTCC ACCCTCATCT GCTACGACAA GATCTTCTGC 
GACATCACTT ACACAGTGAC GGCATGCACG GAGAACGAAG CGAACCGCTA CGGGCGCTTC 
CTCTGCTCCA TGCTGGAGAC GGTGATGCGG TGGCACAGCG ACAAGGCCAT CTTTGACAAG 
GAGTGTGCCA ACTTCCCCGG CTTCATGACC AAGTTCCGGG GCGCCAGCCA GTCGTCGGAC 
ATTGCCACGG ACCACGTGGA CTACGAGAAC TACCGGCACG TCTGTCACAA GTGGCACTTC 
AAGATCACTA AGGCCCTGGT CATCTGTCTG GAGTCTGGGG ACTACGTCCA GATCCGGAAC 
AGCCTGATTG TGCTCACCAA GGTGCTGCCC CACTTCCCGG TCATGACCAA CTTGGGCCAG 
GCGCTGGAGC GGCGCATCGA GAAGATCCGC CAGGAGGAGA AGGAGAAGAG GCCGGACCTC 
TTTGCTCTTG CCACCGGCTA CGCCGGCCAG CTGAAGGGCA AGAAATCCGA GCTGATCCCG 
GAGCACGAGT TCCACCTCAA GGACAGCAAA GACGGCCGCG CGGTGAGCGC CACCGCACAG 
GTGAAGAGCG CTGCAGATCA CACGGTGACG ACGGCGGCAC ATGCCGCCAC CTCTTCCCAA 
GTGGCGAGTG TCGATGGTGC CAAGCAGAAG CGGGGAGACT CCGTGAGGGA GTCCAACTCG 
TCACCAGCAC TGTCCGGCAA TTCTTCTGGG AAGCTGTCGA GCAGCGGCGG AGGTCTCGTC 
CAGTCTTCGT CTTCGTCGTC GTCGTCCAAG ATGACATCGT CCAGTAGTTC TTCCGCCACC 
CGCAGGGAGT CCAACAGCTT TGGTGCGGTT GTGGAGAGTG CCACGACGGC CGCTTTTCCT 
CGGAGGTCGC TGAGCAAGGA GTCCGGGAGC GGGGACGGAC ACGACAAGGG CGCCGCCACC 
GACCGCAAGG GCAGCTCGCT GTCCTCCCTC CACGAGCAGG ACGTCAGGCC CTCCAAGGAC 
GACGTCCACA AAAATGACCA CTGGCTGGAG CCCGGGGACG CGCCCGGAGA CCACAAGCGG 
CGGCGCGTGG ACGGGGGCAG CAGCAGCGGC GGCAGCAGCA GTGGCAAGGT GGGAGGCAGT 
CCCCGGGGCC GTAGCCTCGG GGGCACGCAG AGCCCCAAGG TCTCGGAACC CGAGGGGAAG 
TCTGAGGACC GCGGGGGAGG GGGGGACCGC CCTTCTGAGA AGTGGGCTCA CCCCAAGGAC 
TCCCACGACC CGGCCACGGG GGACAAGGGC AAGGCCAAGA AGGGCAGCCG CAAGCGGGAC 
CACTCCGACG AGCCCCCGCT CGAGGGCAAG CGCAAGAAGG ACGAGGACGC CTTGAAGAGC 
TCGAGCAAGC GCAACGGGGA GGACTCTAAG GAGAAGGAGC GGTACCGGAG GGAAGAGGAC 
AAGCTGCGAG AGTCCCCCAA GCTGCGAGAG CGCAGGGCCG ACCGGGACGA CAAGAAGCAT 
CATCGATCGA GCAGCAGCAG TGGCAGCTCG ACGTCAACGA AAAAGCGATC ATCCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC8029916 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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