LOC8025157 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC8025157
Entrez Gene ID	8025157
Full Name	Ran-binding protein, putative
Gene Type	protein-coding
Organism	Ixodes scapularis(black-legged tick)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002408157.1	XP_002408201.1	Ran-binding protein, putative
XM_029968416.1	XP_029824276.1	importin-7

Pathways from BioSystems
Gene Ontology

The following LOC8025157 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC8025157 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIa01182	XM_002408157.1 Latest version!	Ixodes scapularis Ran-binding protein, putative, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OIa16851	XM_029968416.1 Latest version!	Ixodes scapularis importin-7 (LOC8025157), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC8025157 ( XM_002408157.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_002408201.1 Ixodes scapularis Ran-binding protein, putative, mRNA.

Price & Availability↑

CloneID	OIa01182
Clone ID Related Accession (Same CDS sequence)	XM_002408157.1
Accession Version	XM_002408157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1245600000000
Organism	Ixodes scapularis(black-legged tick)
Product	Ran-binding protein, putative
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002536706). COMPLETENESS: incomplete on both ends.

ATGGACGCTG GTCAGCTCAT CGAGATCCTC AGAGCGACCA TCGACCCAAA CCAGCGGGAA 
AATGCCGAAA AGCAGCTGGA ACAGATCCAC AAGATCATTG GCTTTGCGCC GTCGCTGCTC 
CAAGTGGTGA TGACCAACGG GCTGGACATG CCCGTGCGCC AGGCCGGAGC CATCTACCTC 
AAGAACCTGG TGACGCAGTT CTGGCAGGAG AAGGAGGCAC CCCAGCCGCC CACGGCGCCG 
CTCCACTTCC ACGTGCACGA GCAGGACCGG GCCATGGTGC GGGACGCCAT CGTGGACGCC 
ATGGTGCATG CGCCGGACCT CATCAGGGTA CAGCTTGCCG TGTGCCTGAC GAACATCCTA 
AAGCACGACT TCCCCGGCCG CTGGACGGGC ATTGTGGACA AGGTGTCCAT CTACCTCCAG 
TCGCCAGAGA GCGCCGGCTG GATGGGCTCT CTCCTCGCCC TCTACCAGCT GGTCAAGAAC 
TATGAGTACA AGAAGCCCGA GGAGAGGGAG CCGCTGCACG AGGCGATGCA GCTGCTACTG 
CCGATGCTCC AGCAGCGGCT AGTGCAGCTG CTGCCAGACG CCAGCGAGGC GTCCGTGCTC 
GTCCAGAAGG AGATCCTCAA AATCTTCTTT GCCCTGGTCC AGTACCACTA TCCCCTGGGG 
CTCATTTCCC GCGACGCCTG CACACAGTGG ATGGAGCTCT TCAGGCTTGT CCTGGACCGC 
CCCGTGCCGG ACGTGGCTAA CCAGGTGGAT GAAGACGAAC GGCCCGACCT TCCCTGGTGG 
AAGTGCAAGA AGTGGGCCTT CCACATCATC ACCCGCATGT TCGAAAGGTA CGGGAGCCCG 
GGCTCGGTGA CGAAGGAGTA CGGGGAGTTT GCGGACTTCT TCCTGAAGGC GTACAGCGAG 
GGCATGCTCC AGGTGGTGCT CAAGGTGCTG GACCAGCACC GCCAGAAGGT CTACATCTCC 
CCCAGGGTGC TCCAGCACGC CCTCAACTAC CTACGGCACG CCGTGAGCCA TGCCTTTTCC 
TGGAAATTCC TCAAGCCACA CATAGTTGGC ATCGTCCTGG AAGTGGTGTT TCCCCTGCTG 
TGCCACACGG ATCAGGACGA CGAGCTGTGG AACACAGACC CCCACGAGTA CATCAGACTC 
AAGTTTGACG TGTTTGAGGA CTTCCTGTCT CCTGTGACGG CGGCCCAGAG CCTCGTGCAC 
ACGGTGTGCA AAAAGCGCAA GGGTGTGCTG CAGAGGGCCA TGGCCTTTGT CGTCTCCCTG 
CTCACCAACC CCAGCTGCTC GGCCCGGCAG AAGGACGGGG CCCTACACCT GGTGGGCACC 
GTGGCCACCC TCCTGCTCAA GCGCAAGATG TACAAGGACC AGATGGAGGC AATGCTGGTG 
ACACACGTGT ACCCCGAATT CGGCAGCCCC CACGGCTTCC TGCGGGCCCG GGCCTGCTGG 
GTGCTGCACT ACTTCTGCGA GATCCAGTTC CGGACGGAGG GGAACCTGCT TCGGGCGGTG 
GAGCTGCTCA CCCACTGCCT GCTGCGCGAC GCAGACCTCC CGGTCAAGGT GGAGGCTGCT 
GTGGCGCTCC AGGCCTGCCT CACCTGCCAG GAGAAGGCGC AAGCCAGCGT GGAGCCCCAG 
ATCAAGGAGA TCGCTCTGGA GCTGCTCAAG ATAATCCGAG AAACCGAGAA CGACGACCTG 
ACGAGCGTCA TGCAGAAGAT CGTCTGCATG TACACCGAGC AGATCGTGCC CATCGCCGTC 
GAGATGACCC AACACCTTGC GGCAACCTTC AACATGGTGA TAGAAAGCGG CGGGGAGGGG 
GACGAGAAGG CCATGGTTGC CATGGGGGTG CTGAACACCC TGGACACCCT GGTGACGGTG 
CTGGAAGACC AGCGGGAGCT AGTTTCCCTG CTGGAGCCCA CCCTGCTGCA GCTGCTGGTG 
GGGCAGGTGC TGGGGGCCTC TCTGCTCGAG TTCTACGAGG AGGCCTTTTC CCTGCTCTAC 
TCGCTCTCCT GCAAGGGCCC CCTTTCGGCC GACATGTGGA AGGCCTTCGA GGCCCTCTAC 
CACACCTTCC AGAAGGATGG CTTCGACTTC TTCACCGACA TGATGCCTGC CCTGCACAAC 
TTTGTCACTG TGGACACCCC AGCCTTTGTG AGCAATGAAA ACCACCTCCT GGCCATGTAC 
AACATGAGCA AAGCAGTGCT GGAGGGTGAT TCTGGTGAGG ACCCCAAATT CCATGCACTC 
AAGCTGCTCG AAGTCATCAT CCTCCAGTGC AAGGGACAAA TTGACCAGTG CATCCCTTCC 
TTTGTGGAGC TGGCGATCAG GACGCTAGCA GATTCGGGGG CCTGGGTGGA GCTCCAGGTC 
ATGTGCCAGC AGGTGGTGAT TGCTGCACTC TACTACAATC CGGTCCTCCT GTTTGAGACG 
CTCGGCAAGA TCCAGAGGGC GGACACGTCG GAACATCTGG TGGATCACTT CGTGAAGATG 
TGGCTGGGCA ACATCGAGTA CTTCCTCGGG CTGCATGACC GCAAGATGTG CGTGTTGGGC 
CTGTGCACGC TCATCAGCAG CCAGGGGTCC CGGCCACAGT CCCTGACCGA GATGGCCTCC 
CAGGTGGTGC CCTCCCTGCT CGTGCTTTTC GACGGCCTCA AGCGGGCCTA CGCCTGTGAG 
GCCCAGGACG ACGAAGATTC CGACGACGAG GAAGAGGAAG ACGACGACTA CGAGGGAGGT 
GAGCTAGCGC GGCAGGCCAA GAAGGAGTCC CCGTTTCCAG TCACGTCTGC CACCATCGAG 
GACGACCAGG AGGACGACGA CGAGGATGAC GAGGAAGATG ACGACGAGGA GGAGACAGCG 
CTGGAGTCGT TCACGACGCC CCTGGACGAG GAGGACTGCC CCGTGGACGA GTACATGGTC 
TTCAAGGAGG TCATGCAGGC CATCCAAAGC ACGGACCCCA TGTGGTTCAA CGCCCTGACG 
TCCGGTCTCT CGGGCGAGCA GCAGAAAGCC TTACAAGAGG TCTACCTCCT GGCCGACCAG 
AGGAGAGCAG CGGCAGAGTC CAGACGAATC GAGAAGAGCG GCGGTTACGT GTTCCAGAAC 
CAGACGGTGC CGACGTCTTT CAACTTCGGT GGTGCTCCCC TCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002408201.1
CDS	1..3108
Translation	MDAGQLIEILRATIDPNQRENAEKQLEQIHKIIGFAPSLLQVVMTNGLDMPVRQAGAIYLKNLVTQFWQEKEAPQPPTAPLHFHVHEQDRAMVRDAIVDAMVHAPDLIRVQLAVCLTNILKHDFPGRWTGIVDKVSIYLQSPESAGWMGSLLALYQLVKNYEYKKPEEREPLHEAMQLLLPMLQQRLVQLLPDASEASVLVQKEILKIFFALVQYHYPLGLISRDACTQWMELFRLVLDRPVPDVANQVDEDERPDLPWWKCKKWAFHIITRMFERYGSPGSVTKEYGEFADFFLKAYSEGMLQVVLKVLDQHRQKVYISPRVLQHALNYLRHAVSHAFSWKFLKPHIVGIVLEVVFPLLCHTDQDDELWNTDPHEYIRLKFDVFEDFLSPVTAAQSLVHTVCKKRKGVLQRAMAFVVSLLTNPSCSARQKDGALHLVGTVATLLLKRKMYKDQMEAMLVTHVYPEFGSPHGFLRARACWVLHYFCEIQFRTEGNLLRAVELLTHCLLRDADLPVKVEAAVALQACLTCQEKAQASVEPQIKEIALELLKIIRETENDDLTSVMQKIVCMYTEQIVPIAVEMTQHLAATFNMVIESGGEGDEKAMVAMGVLNTLDTLVTVLEDQRELVSLLEPTLLQLLVGQVLGASLLEFYEEAFSLLYSLSCKGPLSADMWKAFEALYHTFQKDGFDFFTDMMPALHNFVTVDTPAFVSNENHLLAMYNMSKAVLEGDSGEDPKFHALKLLEVIILQCKGQIDQCIPSFVELAIRTLADSGAWVELQVMCQQVVIAALYYNPVLLFETLGKIQRADTSEHLVDHFVKMWLGNIEYFLGLHDRKMCVLGLCTLISSQGSRPQSLTEMASQVVPSLLVLFDGLKRAYACEAQDDEDSDDEEEEDDDYEGGELARQAKKESPFPVTSATIEDDQEDDDEDDEEDDDEEETALESFTTPLDEEDCPVDEYMVFKEVMQAIQSTDPMWFNALTSGLSGEQQKALQEVYLLADQRRAAAESRRIEKSGGYVFQNQTVPTSFNFGGAPLC

Target ORF information:

RefSeq Version	XM_002408157.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis Ran-binding protein, putative, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002408157.1

ORF Insert Sequence:

ATGGACGCTG GTCAGCTCAT CGAGATCCTC AGAGCGACCA TCGACCCAAA CCAGCGGGAA 
AATGCCGAAA AGCAGCTGGA ACAGATCCAC AAGATCATTG GCTTTGCGCC GTCGCTGCTC 
CAAGTGGTGA TGACCAACGG GCTGGACATG CCCGTGCGCC AGGCCGGAGC CATCTACCTC 
AAGAACCTGG TGACGCAGTT CTGGCAGGAG AAGGAGGCAC CCCAGCCGCC CACGGCGCCG 
CTCCACTTCC ACGTGCACGA GCAGGACCGG GCCATGGTGC GGGACGCCAT CGTGGACGCC 
ATGGTGCATG CGCCGGACCT CATCAGGGTA CAGCTTGCCG TGTGCCTGAC GAACATCCTA 
AAGCACGACT TCCCCGGCCG CTGGACGGGC ATTGTGGACA AGGTGTCCAT CTACCTCCAG 
TCGCCAGAGA GCGCCGGCTG GATGGGCTCT CTCCTCGCCC TCTACCAGCT GGTCAAGAAC 
TATGAGTACA AGAAGCCCGA GGAGAGGGAG CCGCTGCACG AGGCGATGCA GCTGCTACTG 
CCGATGCTCC AGCAGCGGCT AGTGCAGCTG CTGCCAGACG CCAGCGAGGC GTCCGTGCTC 
GTCCAGAAGG AGATCCTCAA AATCTTCTTT GCCCTGGTCC AGTACCACTA TCCCCTGGGG 
CTCATTTCCC GCGACGCCTG CACACAGTGG ATGGAGCTCT TCAGGCTTGT CCTGGACCGC 
CCCGTGCCGG ACGTGGCTAA CCAGGTGGAT GAAGACGAAC GGCCCGACCT TCCCTGGTGG 
AAGTGCAAGA AGTGGGCCTT CCACATCATC ACCCGCATGT TCGAAAGGTA CGGGAGCCCG 
GGCTCGGTGA CGAAGGAGTA CGGGGAGTTT GCGGACTTCT TCCTGAAGGC GTACAGCGAG 
GGCATGCTCC AGGTGGTGCT CAAGGTGCTG GACCAGCACC GCCAGAAGGT CTACATCTCC 
CCCAGGGTGC TCCAGCACGC CCTCAACTAC CTACGGCACG CCGTGAGCCA TGCCTTTTCC 
TGGAAATTCC TCAAGCCACA CATAGTTGGC ATCGTCCTGG AAGTGGTGTT TCCCCTGCTG 
TGCCACACGG ATCAGGACGA CGAGCTGTGG AACACAGACC CCCACGAGTA CATCAGACTC 
AAGTTTGACG TGTTTGAGGA CTTCCTGTCT CCTGTGACGG CGGCCCAGAG CCTCGTGCAC 
ACGGTGTGCA AAAAGCGCAA GGGTGTGCTG CAGAGGGCCA TGGCCTTTGT CGTCTCCCTG 
CTCACCAACC CCAGCTGCTC GGCCCGGCAG AAGGACGGGG CCCTACACCT GGTGGGCACC 
GTGGCCACCC TCCTGCTCAA GCGCAAGATG TACAAGGACC AGATGGAGGC AATGCTGGTG 
ACACACGTGT ACCCCGAATT CGGCAGCCCC CACGGCTTCC TGCGGGCCCG GGCCTGCTGG 
GTGCTGCACT ACTTCTGCGA GATCCAGTTC CGGACGGAGG GGAACCTGCT TCGGGCGGTG 
GAGCTGCTCA CCCACTGCCT GCTGCGCGAC GCAGACCTCC CGGTCAAGGT GGAGGCTGCT 
GTGGCGCTCC AGGCCTGCCT CACCTGCCAG GAGAAGGCGC AAGCCAGCGT GGAGCCCCAG 
ATCAAGGAGA TCGCTCTGGA GCTGCTCAAG ATAATCCGAG AAACCGAGAA CGACGACCTG 
ACGAGCGTCA TGCAGAAGAT CGTCTGCATG TACACCGAGC AGATCGTGCC CATCGCCGTC 
GAGATGACCC AACACCTTGC GGCAACCTTC AACATGGTGA TAGAAAGCGG CGGGGAGGGG 
GACGAGAAGG CCATGGTTGC CATGGGGGTG CTGAACACCC TGGACACCCT GGTGACGGTG 
CTGGAAGACC AGCGGGAGCT AGTTTCCCTG CTGGAGCCCA CCCTGCTGCA GCTGCTGGTG 
GGGCAGGTGC TGGGGGCCTC TCTGCTCGAG TTCTACGAGG AGGCCTTTTC CCTGCTCTAC 
TCGCTCTCCT GCAAGGGCCC CCTTTCGGCC GACATGTGGA AGGCCTTCGA GGCCCTCTAC 
CACACCTTCC AGAAGGATGG CTTCGACTTC TTCACCGACA TGATGCCTGC CCTGCACAAC 
TTTGTCACTG TGGACACCCC AGCCTTTGTG AGCAATGAAA ACCACCTCCT GGCCATGTAC 
AACATGAGCA AAGCAGTGCT GGAGGGTGAT TCTGGTGAGG ACCCCAAATT CCATGCACTC 
AAGCTGCTCG AAGTCATCAT CCTCCAGTGC AAGGGACAAA TTGACCAGTG CATCCCTTCC 
TTTGTGGAGC TGGCGATCAG GACGCTAGCA GATTCGGGGG CCTGGGTGGA GCTCCAGGTC 
ATGTGCCAGC AGGTGGTGAT TGCTGCACTC TACTACAATC CGGTCCTCCT GTTTGAGACG 
CTCGGCAAGA TCCAGAGGGC GGACACGTCG GAACATCTGG TGGATCACTT CGTGAAGATG 
TGGCTGGGCA ACATCGAGTA CTTCCTCGGG CTGCATGACC GCAAGATGTG CGTGTTGGGC 
CTGTGCACGC TCATCAGCAG CCAGGGGTCC CGGCCACAGT CCCTGACCGA GATGGCCTCC 
CAGGTGGTGC CCTCCCTGCT CGTGCTTTTC GACGGCCTCA AGCGGGCCTA CGCCTGTGAG 
GCCCAGGACG ACGAAGATTC CGACGACGAG GAAGAGGAAG ACGACGACTA CGAGGGAGGT 
GAGCTAGCGC GGCAGGCCAA GAAGGAGTCC CCGTTTCCAG TCACGTCTGC CACCATCGAG 
GACGACCAGG AGGACGACGA CGAGGATGAC GAGGAAGATG ACGACGAGGA GGAGACAGCG 
CTGGAGTCGT TCACGACGCC CCTGGACGAG GAGGACTGCC CCGTGGACGA GTACATGGTC 
TTCAAGGAGG TCATGCAGGC CATCCAAAGC ACGGACCCCA TGTGGTTCAA CGCCCTGACG 
TCCGGTCTCT CGGGCGAGCA GCAGAAAGCC TTACAAGAGG TCTACCTCCT GGCCGACCAG 
AGGAGAGCAG CGGCAGAGTC CAGACGAATC GAGAAGAGCG GCGGTTACGT GTTCCAGAAC 
CAGACGGTGC CGACGTCTTT CAACTTCGGT GGTGCTCCCC TCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC8025157 ( XM_029968416.1 ) cDNA ORF clone, Ixodes scapularis(black-legged tick) -> XP_029824276.1 Ixodes scapularis importin-7 (LOC8025157), mRNA.

Price & Availability↑

CloneID	OIa16851
Clone ID Related Accession (Same CDS sequence)	XM_029968416.1
Accession Version	XM_029968416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1564329600000
Organism	Ixodes scapularis(black-legged tick)
Product	importin-7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_021918062.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ixodes scapularis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGCTG GTCAGCTCAT CGAGATCCTC AGAGCGACCA TCGACCCAAA CCAGCGGGAA 
AATGCCGAAA AGCAGCTGGA ACAGATCCAC AAGATCATTG GCTTTGCGCC GTCGCTGCTC 
CAAGTGGTGA TGACCAACGG GCTGGACATG CCCGTGCGCC AGGCCGGAGC CATCTACCTC 
AAGAACCTGG TGACGCAGTT CTGGCAGGAG AAGGAGGCAC CTCAGCCGCC CACAGCGCCA 
CTCCACTTCC ACGTGCATGA GCAGGACCGG GCCATGGTGC GGGACGCCAT CGTGGACGCC 
ATGGTGCATG CGCCGGACCT CATCAGGGTA CAGCTTGCCG TGTGCCTGAC GAACATCCTG 
AAGCATGACT TCCCCGGTCG CTGGACGGGC ATTGTGGACA AGGTGTCCAT CTACCTCCAG 
TCGCCAGAGA GCGCCGGCTG GATGGGCTCC CTCCTCGCCC TCTACCAGCT GGTCAAGAAC 
TATGAGTATA AGAAGCCCGA GGAGAGGGAG CCGCTGCACG AGGCGATGCA GCTGCTACTG 
CCGATGCTCC AGCAGCGGCT AGTGCAGCTG CTGCCAGACG CCAGCGAGGC GTCCGTGCTC 
GTCCAGAAGG AGATCCTCAA AATCTTCTTT GCCCTGGTCC AGTACCACTA TCCCCTGGGG 
CTCATTTCCC GCGACGCCTG CACACAGTGG ATGGAGCTCT TCAGGCTTGT CCTGGACCGC 
CCCGTGCCGG ACGTGGCTAA CCAGGTGGAT GAAGACGAAC GGCCCGACCT TCCCTGGTGG 
AAGTGCAAGA AGTGGGCCTT CCACATCATC ACCCGCATGT TCGAAAGGTA CGGGAGCCCG 
GGCTCGGTGA CGAAGGAGTA CGGGGAGTTT GCGGACTTCT TCCTGAAGGC GTACAGCGAG 
GGCATGCTCC AGGTGGTGCT CAAGGTGCTG GACCAGCACC GCCAGAAGGT CTACATCTCC 
CCCAGGGTGC TCCAGCACGC CCTCAACTAC CTACGGCACG CCGTGAGCCA TGCCTTTTCC 
TGGAAATTCC TCAAGCCACA CATAGTTGGC ATCGTCCTGG AAGTGGTGTT TCCCCTGCTG 
TGCCACACGG ATCAGGACGA CGAGCTGTGG AACACAGACC CCCACGAGTA CATCAGACTC 
AAGTTTGACG TGTTTGAGGA CTTCCTGTCT CCTGTGACGG CGGCCCAGAG CCTCGTGCAC 
ACGGTGTGCA AAAAGCGCAA GGGTGTGCTG CAGAGGGCCA TGGCCTTTGT CGTCTCCCTG 
CTCACCAACC CCAGCTGCTC GGCCCGGCAG AAGGACGGGG CCCTACACCT GGTGGGCACC 
GTGGCCACCC TCCTGCTCAA GCGCAAGATG TACAAGGACC AGATGGAGGC AATGCTGGTG 
ACACACGTGT ACCCCGAATT CGGCAGCCCC CACGGCTTCC TGCGGGCCCG GGCCTGCTGG 
GTGCTGCACT ACTTCTGCGA GATCCAGTTC CGGACGGAGG GGAACCTGCT CCGGGCGGTG 
GAGCTGCTCA CCCACTGCCT GCTGCGCGAC GCAGACCTCC CGGTCAAGGT GGAGGCTGCT 
GTGGCGCTCC AGGCCTGCCT CACCTGCCAG GAGAAGGCAC AAGCCAGCGT GGAGCCCCAG 
ATCAAGGAGA TCGCTCTGGA GCTGCTCAAG ATAATCCGAG AAACCGAGAA CGACGACCTG 
ACGAGCGTCA TGCAGAAGAT CGTCTGCATG TACACCGAGC AGATCGTGCC CATCGCTGTC 
GAGATGACCC AACACCTTGC AGCAACCTTC AACATGGTGA TAGAAAGCGG CGGGGAGGGG 
GACGAGAAGG CCATGGTTGC CATGGGGGTG CTGAACACCC TGGACACCCT GGTGACGGTG 
CTGGAGGACC AGCGGGAGCT AGTTTCCCTG CTGGAGCCCA CCCTGCTGCA GCTGCTGGTG 
GGGCAGGTGC TGGGGGCCTC TCTGCTCGAG TTCTACGAGG AGGCCTTTTC CCTGCTCTAC 
TCGCTCTCCT GCAAGGGCCC CCTTTCGGCC GACATGTGGA AGGCCTTCGA GGCCCTCTAC 
CACACCTTCC AGAAGGATGG CTTCGACTTC TTCACCGACA TGATGCCTGC CCTGCACAAC 
TTTGTCACCG TGGACACCCC AGCCTTTGTG AGCAATGAAA ACCACCTCCT GGCCATGTAC 
AACATGAGCA AAGCAGTGCT GGAGGGTGAT TCTGGTGAGG ACCCCAAATT CCATGCACTC 
AAGCTGCTCG AAGTCATCAT CCTCCAGTGC AAGGGACAAA TTGACCAGTG CATCCCTTCC 
TTTGTGGAGC TGGCGATCAG GACGCTAGCA GATTCAGGGG CCTGGGTGGA GCTCCAAGTC 
ATGTGCCAGC AGGTGGTGAT TGCTGCACTC TACTACAATC CGGTCCTCCT GTTTGAGACG 
CTCGGCAAGA TCCAGAGGGC GGACACGTCG GAACATCTGG TGGATCACTT CGTGAAGATG 
TGGCTGGGCA ACATCGAGTA CTTCCTCGGG CTGCACGACC GCAAGATGTG CGTGTTGGGC 
CTGTGCACGC TCATCAGCAG CCAGGGGTCC CGGCCGCAGT CCCTGACCGA GATGGCCTCC 
CAGGTGGTGC CCTCCCTGCT CGTGCTTTTC GACGGCCTCA AGCGGGCCTA CGCCTACAGG 
GCCCAGGACG ACGAAGATTC CGACGACGAG GAAGAGGAAG ACGACGACTA CGAGGGAGAG 
GTGCTGGACA GTGACGAGGA CGACGTCGAG GGCCAGGACT ACCTGCAGAG GCTAGCGCGG 
CAGGCCAAGA AGGAGTCCCC ATTTCCGGTC ACGTCTGCCA CCATCGAGGA CGACCAGGAG 
GACGACGACG AGGACGACGA GGAAGATGAC GACGAGGAGG AGACAGCGCT GGAGTCGTTC 
ATGACGCCCC TGGACGAGGA GGACTGCCCC GTGGACGAGT ACATGGTCTT CAAGGAGGTC 
ATGCAGGCCA TCCAAAGCAC GGACCCCATG TGGTTCAACG CCCTGACGTC CGGTCTCTCG 
GGTGAGCAGC AGAAAGCCTT ACAAGAGGTC TACCTCCTGG CCGACCAGAG GAGAGCAGCG 
GCAGAGTCCA GACGAATCGA GAAGAGCGGC GGTTACGTGT TCCAGAACCA GACGGTGCCG 
ACGTCTTTCA ACTTCGGTGG TGCTCCCCTC TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029824276.1
CDS	88..3243
Translation	MDAGQLIEILRATIDPNQRENAEKQLEQIHKIIGFAPSLLQVVMTNGLDMPVRQAGAIYLKNLVTQFWQEKEAPQPPTAPLHFHVHEQDRAMVRDAIVDAMVHAPDLIRVQLAVCLTNILKHDFPGRWTGIVDKVSIYLQSPESAGWMGSLLALYQLVKNYEYKKPEEREPLHEAMQLLLPMLQQRLVQLLPDASEASVLVQKEILKIFFALVQYHYPLGLISRDACTQWMELFRLVLDRPVPDVANQVDEDERPDLPWWKCKKWAFHIITRMFERYGSPGSVTKEYGEFADFFLKAYSEGMLQVVLKVLDQHRQKVYISPRVLQHALNYLRHAVSHAFSWKFLKPHIVGIVLEVVFPLLCHTDQDDELWNTDPHEYIRLKFDVFEDFLSPVTAAQSLVHTVCKKRKGVLQRAMAFVVSLLTNPSCSARQKDGALHLVGTVATLLLKRKMYKDQMEAMLVTHVYPEFGSPHGFLRARACWVLHYFCEIQFRTEGNLLRAVELLTHCLLRDADLPVKVEAAVALQACLTCQEKAQASVEPQIKEIALELLKIIRETENDDLTSVMQKIVCMYTEQIVPIAVEMTQHLAATFNMVIESGGEGDEKAMVAMGVLNTLDTLVTVLEDQRELVSLLEPTLLQLLVGQVLGASLLEFYEEAFSLLYSLSCKGPLSADMWKAFEALYHTFQKDGFDFFTDMMPALHNFVTVDTPAFVSNENHLLAMYNMSKAVLEGDSGEDPKFHALKLLEVIILQCKGQIDQCIPSFVELAIRTLADSGAWVELQVMCQQVVIAALYYNPVLLFETLGKIQRADTSEHLVDHFVKMWLGNIEYFLGLHDRKMCVLGLCTLISSQGSRPQSLTEMASQVVPSLLVLFDGLKRAYAYRAQDDEDSDDEEEEDDDYEGEVLDSDEDDVEGQDYLQRLARQAKKESPFPVTSATIEDDQEDDDEDDEEDDDEEETALESFMTPLDEEDCPVDEYMVFKEVMQAIQSTDPMWFNALTSGLSGEQQKALQEVYLLADQRRAAAESRRIEKSGGYVFQNQTVPTSFNFGGAPLC

Target ORF information:

RefSeq Version	XM_029968416.1
Organism	Ixodes scapularis(black-legged tick)
Definition	Ixodes scapularis importin-7 (LOC8025157), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029968416.1

ORF Insert Sequence:

ATGGACGCTG GTCAGCTCAT CGAGATCCTC AGAGCGACCA TCGACCCAAA CCAGCGGGAA 
AATGCCGAAA AGCAGCTGGA ACAGATCCAC AAGATCATTG GCTTTGCGCC GTCGCTGCTC 
CAAGTGGTGA TGACCAACGG GCTGGACATG CCCGTGCGCC AGGCCGGAGC CATCTACCTC 
AAGAACCTGG TGACGCAGTT CTGGCAGGAG AAGGAGGCAC CTCAGCCGCC CACAGCGCCA 
CTCCACTTCC ACGTGCATGA GCAGGACCGG GCCATGGTGC GGGACGCCAT CGTGGACGCC 
ATGGTGCATG CGCCGGACCT CATCAGGGTA CAGCTTGCCG TGTGCCTGAC GAACATCCTG 
AAGCATGACT TCCCCGGTCG CTGGACGGGC ATTGTGGACA AGGTGTCCAT CTACCTCCAG 
TCGCCAGAGA GCGCCGGCTG GATGGGCTCC CTCCTCGCCC TCTACCAGCT GGTCAAGAAC 
TATGAGTATA AGAAGCCCGA GGAGAGGGAG CCGCTGCACG AGGCGATGCA GCTGCTACTG 
CCGATGCTCC AGCAGCGGCT AGTGCAGCTG CTGCCAGACG CCAGCGAGGC GTCCGTGCTC 
GTCCAGAAGG AGATCCTCAA AATCTTCTTT GCCCTGGTCC AGTACCACTA TCCCCTGGGG 
CTCATTTCCC GCGACGCCTG CACACAGTGG ATGGAGCTCT TCAGGCTTGT CCTGGACCGC 
CCCGTGCCGG ACGTGGCTAA CCAGGTGGAT GAAGACGAAC GGCCCGACCT TCCCTGGTGG 
AAGTGCAAGA AGTGGGCCTT CCACATCATC ACCCGCATGT TCGAAAGGTA CGGGAGCCCG 
GGCTCGGTGA CGAAGGAGTA CGGGGAGTTT GCGGACTTCT TCCTGAAGGC GTACAGCGAG 
GGCATGCTCC AGGTGGTGCT CAAGGTGCTG GACCAGCACC GCCAGAAGGT CTACATCTCC 
CCCAGGGTGC TCCAGCACGC CCTCAACTAC CTACGGCACG CCGTGAGCCA TGCCTTTTCC 
TGGAAATTCC TCAAGCCACA CATAGTTGGC ATCGTCCTGG AAGTGGTGTT TCCCCTGCTG 
TGCCACACGG ATCAGGACGA CGAGCTGTGG AACACAGACC CCCACGAGTA CATCAGACTC 
AAGTTTGACG TGTTTGAGGA CTTCCTGTCT CCTGTGACGG CGGCCCAGAG CCTCGTGCAC 
ACGGTGTGCA AAAAGCGCAA GGGTGTGCTG CAGAGGGCCA TGGCCTTTGT CGTCTCCCTG 
CTCACCAACC CCAGCTGCTC GGCCCGGCAG AAGGACGGGG CCCTACACCT GGTGGGCACC 
GTGGCCACCC TCCTGCTCAA GCGCAAGATG TACAAGGACC AGATGGAGGC AATGCTGGTG 
ACACACGTGT ACCCCGAATT CGGCAGCCCC CACGGCTTCC TGCGGGCCCG GGCCTGCTGG 
GTGCTGCACT ACTTCTGCGA GATCCAGTTC CGGACGGAGG GGAACCTGCT CCGGGCGGTG 
GAGCTGCTCA CCCACTGCCT GCTGCGCGAC GCAGACCTCC CGGTCAAGGT GGAGGCTGCT 
GTGGCGCTCC AGGCCTGCCT CACCTGCCAG GAGAAGGCAC AAGCCAGCGT GGAGCCCCAG 
ATCAAGGAGA TCGCTCTGGA GCTGCTCAAG ATAATCCGAG AAACCGAGAA CGACGACCTG 
ACGAGCGTCA TGCAGAAGAT CGTCTGCATG TACACCGAGC AGATCGTGCC CATCGCTGTC 
GAGATGACCC AACACCTTGC AGCAACCTTC AACATGGTGA TAGAAAGCGG CGGGGAGGGG 
GACGAGAAGG CCATGGTTGC CATGGGGGTG CTGAACACCC TGGACACCCT GGTGACGGTG 
CTGGAGGACC AGCGGGAGCT AGTTTCCCTG CTGGAGCCCA CCCTGCTGCA GCTGCTGGTG 
GGGCAGGTGC TGGGGGCCTC TCTGCTCGAG TTCTACGAGG AGGCCTTTTC CCTGCTCTAC 
TCGCTCTCCT GCAAGGGCCC CCTTTCGGCC GACATGTGGA AGGCCTTCGA GGCCCTCTAC 
CACACCTTCC AGAAGGATGG CTTCGACTTC TTCACCGACA TGATGCCTGC CCTGCACAAC 
TTTGTCACCG TGGACACCCC AGCCTTTGTG AGCAATGAAA ACCACCTCCT GGCCATGTAC 
AACATGAGCA AAGCAGTGCT GGAGGGTGAT TCTGGTGAGG ACCCCAAATT CCATGCACTC 
AAGCTGCTCG AAGTCATCAT CCTCCAGTGC AAGGGACAAA TTGACCAGTG CATCCCTTCC 
TTTGTGGAGC TGGCGATCAG GACGCTAGCA GATTCAGGGG CCTGGGTGGA GCTCCAAGTC 
ATGTGCCAGC AGGTGGTGAT TGCTGCACTC TACTACAATC CGGTCCTCCT GTTTGAGACG 
CTCGGCAAGA TCCAGAGGGC GGACACGTCG GAACATCTGG TGGATCACTT CGTGAAGATG 
TGGCTGGGCA ACATCGAGTA CTTCCTCGGG CTGCACGACC GCAAGATGTG CGTGTTGGGC 
CTGTGCACGC TCATCAGCAG CCAGGGGTCC CGGCCGCAGT CCCTGACCGA GATGGCCTCC 
CAGGTGGTGC CCTCCCTGCT CGTGCTTTTC GACGGCCTCA AGCGGGCCTA CGCCTACAGG 
GCCCAGGACG ACGAAGATTC CGACGACGAG GAAGAGGAAG ACGACGACTA CGAGGGAGAG 
GTGCTGGACA GTGACGAGGA CGACGTCGAG GGCCAGGACT ACCTGCAGAG GCTAGCGCGG 
CAGGCCAAGA AGGAGTCCCC ATTTCCGGTC ACGTCTGCCA CCATCGAGGA CGACCAGGAG 
GACGACGACG AGGACGACGA GGAAGATGAC GACGAGGAGG AGACAGCGCT GGAGTCGTTC 
ATGACGCCCC TGGACGAGGA GGACTGCCCC GTGGACGAGT ACATGGTCTT CAAGGAGGTC 
ATGCAGGCCA TCCAAAGCAC GGACCCCATG TGGTTCAACG CCCTGACGTC CGGTCTCTCG 
GGTGAGCAGC AGAAAGCCTT ACAAGAGGTC TACCTCCTGG CCGACCAGAG GAGAGCAGCG 
GCAGAGTCCA GACGAATCGA GAAGAGCGGC GGTTACGTGT TCCAGAACCA GACGGTGCCG 
ACGTCTTTCA ACTTCGGTGG TGCTCCCCTC TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC8025157 cDNA ORF clone, Ixodes scapularis(black-legged tick)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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