LOC100214119 cDNA ORF clone, Hydra vulgaris

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100214119
Entrez Gene ID	100214119
Full Name	ras and EF-hand domain-containing protein homolog
Gene Type	protein-coding
Organism	Hydra vulgaris

mRNA and Protein(s)

mRNA	Protein	Name
XM_012705291.1	XP_012560745.1	uncharacterized protein LOC100214119 isoform X1
XM_012705299.1	XP_012560753.1	uncharacterized protein LOC100214119 isoform X3
XM_012705293.1	XP_012560747.1	uncharacterized protein LOC100214119 isoform X2
XM_012705303.1	XP_012560757.1	ras and EF-hand domain-containing protein homolog isoform X4

Pathways from BioSystems
Gene Ontology

The following LOC100214119 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100214119 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHc18048	XM_012705291.1 Latest version!	Hydra vulgaris ras and EF-hand domain-containing protein homolog (LOC100214119), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHc18049	XM_012705293.1 Latest version!	Hydra vulgaris ras and EF-hand domain-containing protein homolog (LOC100214119), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHc18050	XM_012705299.1 Latest version!	Hydra vulgaris ras and EF-hand domain-containing protein homolog (LOC100214119), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHc18051	XM_012705303.1 Latest version!	Hydra vulgaris ras and EF-hand domain-containing protein homolog (LOC100214119), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100214119 ( XM_012705291.1 ) cDNA ORF clone, Hydra vulgaris -> XP_012560745.1 Hydra vulgaris ras and EF-hand domain-containing protein homolog (LOC100214119), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHc18048
Clone ID Related Accession (Same CDS sequence)	XM_012705291.1
Accession Version	XM_012705291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433606400000
Organism	Hydra vulgaris
Product	uncharacterized protein LOC100214119 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004166982.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hydra vulgaris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAG AATTTGTTGA AGTAACGTTT TCTGTGCCAA GAAATAGTTT TTTTCAATAT 
TCACCCAATG TTAATGGAAA CACACATGTT GTTCCTGATA ATAACTACAA TAGTTCTCTA 
AACAATCGTT CTTATTGCTA TGTACAAGAT CATCTTGCAG TAGAAAAAGA ATCATTCTAT 
ACCAATTTTT ATGATTGTAC CAATACAACG CATGCAAGTA AAGAATCTTG TGACCTCAAT 
AACTTATCAA CCAAAAATCT TTTCCAAAAA AATTCTGACA CTCGAACTTC TTGCACTATG 
TACGAATCAA TAAAACAACC ACACAATTCT TCAATACAAA TAAAATCACT CGGTGTCACA 
CCGTGGCGCC TTTTTCCTCA AAATGACAGC ATCTTTACTA GTTCGCCTCT CTGTTTCAAT 
AAAGATAATG TATCTCAAAT TAAACCTAAA ATCTTACTTT CTCAAAAAGA GGTGCCAACT 
TTTGAAGAGT TTTTAAATAA GATACATCCT AGATGGGCAT CTTATTTGCC AGGACAGGAA 
TACCTTGATG ATTTATATGA GATTGCCAGA TCCAGAGATG ATCCGCTGCT TCTTGATTCC 
TTGGAAAATT TTCTTGCTGC AACAGTAGAT AATTTATTGA GAAAACAGGC AGAAAATGAA 
TACTTGGAGC TTTCTCTTAA AAGAGCAACA TTAAAGAATA ATGAGTTTAG TTCGCAGCTT 
GAACAAGAAA TGGATCAACA TGTTCAAATG CTGGAGGAGA AAATTCGACA AGAAGAACAG 
CAAAAACTGG CTATTCAAAA ACAAGAAGCC TATCAACAGT TAATAATTGC TCAAAAGGAA 
CTGGCTGAAA TGGAGAATAA AGTTAAAATG CTAGAAAAAC AAGTCGAGGA GAAATCAGTT 
AAAACTGTCA TTCCTAATAC AATTTTAGAA GAAATAAAGC TGAAGGAAAA GGAAAATGAA 
ACTCTTCATC TTCGTTTGCT TGAATTTCAA ACACAAATGG CTCTTATGAG AACTGAAGTT 
GCAAAACTTA AGTGTGAATA TGATGAACAA TCTGATCGTC TTCAAAAAGA GCGTGTAACT 
GTTTTAGATT GTGTCCAAGA GCAAGAAAAT TTAACACGAC AATTACATTT GCTACATGAG 
GCAAATAAAA AGTTGCACGA TACTAATGAT GATCTTCGTT CAGCAATGGA GGTCAAGCGC 
TCGAACAGTT ATTTTATTGG CTCTCCAGTA AAACGTACTA CAAGTTTGGT GTTAACTAAG 
TATAGACAGC CGAATTTGCA CAGTTCATTC CGAAGTGATC CTGGACCAGA TTTAAATCTT 
AATGACTTTG ATGACACAGA TATTGAGGAT GCTTCAGGAC GATCAATTAT ACCTTACGAT 
GAAGAAGTCA TTGACAAATC TTCACTCATG GCAGAATTAA TGGAGGCTGA TGACCAAGTT 
CATAATGGCT ATATGTCTGG AAAAAAACCT GTTAGAGTAC TCAGTCTTGA AAACTCTGCT 
TCATCTACCC CTCAAAAACA TGTTGATAGA GAGCGGTTTG TACGTCAACT AGAGGTTTTG 
TGCGAAACAA ATAGAAGATT AGGTGAAAGT AATGATGAAC TTAGAGAAGC ACTAGGAATT 
TTATCAGAGC GACTGCGACC ACCAGATTTG ATAAAAAAAT CCTCTGGTTG TCAATCAGCT 
CCAGTGACTC CTTCAAGAAG AATTTACAGC TCTCCCATCT CCAGCTCTCC TCAAAAGTCG 
GCAGAATCAT CCCACAAAGG TTTTGTTGAC ACAATGAAAA AGTCTGCTGA ATCATCCCAC 
AAAGTTTTTG TTGATACTGC AAAAAAAGAA GTAAAACTTC AATGTTTTAC TCCTTTTAAT 
GAGTCTTCTG TGGATTCTAC TACATTTAAC ACTGATGATG CTACTGAATC TTCAATCTTA 
AAAGGAGAAT ATCATAGAAG AATGGTTGAT GATCGCAGTT ATAATAATAA TAATAGTTTA 
ACAGATTCAA GTAATGAAGC TACAAACATT GAATCTACTG CATATGATGA TGAATCTACT 
GTTTATGATG GGGAATCAAC TGTCTATGAT GGAGAATCAA CTGTCTATGA TGGAGAATCT 
ACTGTTTTAA ACACCTGTTG TTGTGGTAAA ATAATTTTTG AAGACAAAAA TTTGTGCAAA 
GATTGCACTT TTACAGACAC TGAAAAAGGC ACTGGAATTT CAGATACATT TTTGTCAAGT 
GATCAGAGTT TGTTTGATAA TATTCACACT GAAAGTTCTG ACTTGAATAG TTTACATGTC 
CTACCAGCAA AAACTGATAT GTGCACTCAA GTAGATAATA ATCTTAGTTT ATGTGCTATT 
TCTGAAAAAC ATGAAGTTTC AGTTCAGTGT GAATTCCCTC GTGAACTTCC AAGTAAAAAT 
GAACTAAATG TCAATCAGTC TTTTGAACAT AATAAAACAA AATTGCAAAG AAAAGAAGCA 
AAAATGAATA ATCATTTACT AAACAAAACC CAATCAACAG AATATCAGTT TAAAGAAATA 
GATGAGATAG TGCATGATAT AGTTGAATCA AGTCTTGATA AAGCTGATAG TATAAGCTTG 
GATCATATTT ATGAAAAACA CAATAGTAAG GAAGTTAGTC AACAAATTTC TGAAAAACCT 
TACTATATAT CTGACTATAA TTTTTATGAC ATATTTAATG ACCATTCGAT AAATCAAGTA 
CCTGAGTCAA CAATTCTTAC AGACTCTGAT GATAGCAATA ATTATAGTGA TCAAGAAACA 
GGGATTGATA TGAGCATGGT AAAACGTTTC AAACCAGTTG GCGAAGAGAG TAATGAGGAC 
GCTACTTACG AAACTACTAA TGAATACATG ACTAACTACT CTTGGTCTGT AAGCTCTGGA 
TCTGCATTTT TATTGAAAAA AAAAAAAAAA GAGTTAAGCA TACGCTCACA ACGCCAGTTT 
GATGAAGATG TTGATGATTA TAATATGCCA GATGAAGAAC TTGCTCGTTT AGTTAATCAA 
ACAAATGATG ACGATTTTTC AAGCACTAGT GAGGAATTAA TCACTGTAGA AGAAAAAATT 
ATTGAGCCAT GTGAAGCTGC AAAGAATCAT GTCCTGAAGC GTGAGTTTGG GGTTGGTGCA 
GATACTTCAG GTGAATCATT ATCTGCAAAG GAAGGAGATG CAACTGTTCC AGAGAGAATG 
TATAAATTAG TTTTAGTTGG AAATGCAGCT GTTGGAAAAT CAAGTTTTAT TATTCGTTTA 
TGCAAGAATA AATTCTATTC TTCTCTTAAC TCAACTCTTG GTGTTGATTT TCAAATAAAA 
AACCTCCAAT TAAATGGAAA AACAGTAGCT CTGCAGCTCT GGGACACGGC TGGACAAGAA 
AGGTTTCGAA GTATTGCAAA AAGTTACTTT CGAAAAGTTG ATGGCGTTAT ACTCATGTAT 
GATGTAACAT GTGAAAGTTC TTTTATGGAT GTTCGAGATT GGCTGGAATC TATAAAAGAG 
GGATCCTCAA AAACAGTTCC TATCATAATT TGCGGCAATA AAACGGACCT TCGACTTGAT 
GCACGTCGGA AAGGTATGCC AGTTGTCAGT CAAGAACAAG GTCAAAGGTT AGCAAATGAC 
ATTGGAGCTC TTTTTATTGA AACCAGCGCA AAAGAAGGAA CAAACATCAC TAACACATGC 
ATAGAATTAA CCAAACAATT AATTCAAAAT GATGAAAAAC GATTGTCTGC CACAGGAATG 
CAACTAAATA AACAGCCGAG TCAAACAAAA AAAAATGGTT GCTGTCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012560745.1
CDS	142..3912
Translation	MNSEFVEVTFSVPRNSFFQYSPNVNGNTHVVPDNNYNSSLNNRSYCYVQDHLAVEKESFYTNFYDCTNTTHASKESCDLNNLSTKNLFQKNSDTRTSCTMYESIKQPHNSSIQIKSLGVTPWRLFPQNDSIFTSSPLCFNKDNVSQIKPKILLSQKEVPTFEEFLNKIHPRWASYLPGQEYLDDLYEIARSRDDPLLLDSLENFLAATVDNLLRKQAENEYLELSLKRATLKNNEFSSQLEQEMDQHVQMLEEKIRQEEQQKLAIQKQEAYQQLIIAQKELAEMENKVKMLEKQVEEKSVKTVIPNTILEEIKLKEKENETLHLRLLEFQTQMALMRTEVAKLKCEYDEQSDRLQKERVTVLDCVQEQENLTRQLHLLHEANKKLHDTNDDLRSAMEVKRSNSYFIGSPVKRTTSLVLTKYRQPNLHSSFRSDPGPDLNLNDFDDTDIEDASGRSIIPYDEEVIDKSSLMAELMEADDQVHNGYMSGKKPVRVLSLENSASSTPQKHVDRERFVRQLEVLCETNRRLGESNDELREALGILSERLRPPDLIKKSSGCQSAPVTPSRRIYSSPISSSPQKSAESSHKGFVDTMKKSAESSHKVFVDTAKKEVKLQCFTPFNESSVDSTTFNTDDATESSILKGEYHRRMVDDRSYNNNNSLTDSSNEATNIESTAYDDESTVYDGESTVYDGESTVYDGESTVLNTCCCGKIIFEDKNLCKDCTFTDTEKGTGISDTFLSSDQSLFDNIHTESSDLNSLHVLPAKTDMCTQVDNNLSLCAISEKHEVSVQCEFPRELPSKNELNVNQSFEHNKTKLQRKEAKMNNHLLNKTQSTEYQFKEIDEIVHDIVESSLDKADSISLDHIYEKHNSKEVSQQISEKPYYISDYNFYDIFNDHSINQVPESTILTDSDDSNNYSDQETGIDMSMVKRFKPVGEESNEDATYETTNEYMTNYSWSVSSGSAFLLKKKKKELSIRSQRQFDEDVDDYNMPDEELARLVNQTNDDDFSSTSEELITVEEKIIEPCEAAKNHVLKREFGVGADTSGESLSAKEGDATVPERMYKLVLVGNAAVGKSSFIIRLCKNKFYSSLNSTLGVDFQIKNLQLNGKTVALQLWDTAGQERFRSIAKSYFRKVDGVILMYDVTCESSFMDVRDWLESIKEGSSKTVPIIICGNKTDLRLDARRKGMPVVSQEQGQRLANDIGALFIETSAKEGTNITNTCIELTKQLIQNDEKRLSATGMQLNKQPSQTKKNGCCR

Target ORF information:

RefSeq Version	XM_012705291.1
Organism	Hydra vulgaris
Definition	Hydra vulgaris ras and EF-hand domain-containing protein homolog (LOC100214119), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012705291.1

ORF Insert Sequence:

ATGAACTCAG AATTTGTTGA AGTAACGTTT TCTGTGCCAA GAAATAGTTT TTTTCAATAT 
TCACCCAATG TTAATGGAAA CACACATGTT GTTCCTGATA ATAACTACAA TAGTTCTCTA 
AACAATCGTT CTTATTGCTA TGTACAAGAT CATCTTGCAG TAGAAAAAGA ATCATTCTAT 
ACCAATTTTT ATGATTGTAC CAATACAACG CATGCAAGTA AAGAATCTTG TGACCTCAAT 
AACTTATCAA CCAAAAATCT TTTCCAAAAA AATTCTGACA CTCGAACTTC TTGCACTATG 
TACGAATCAA TAAAACAACC ACACAATTCT TCAATACAAA TAAAATCACT CGGTGTCACA 
CCGTGGCGCC TTTTTCCTCA AAATGACAGC ATCTTTACTA GTTCGCCTCT CTGTTTCAAT 
AAAGATAATG TATCTCAAAT TAAACCTAAA ATCTTACTTT CTCAAAAAGA GGTGCCAACT 
TTTGAAGAGT TTTTAAATAA GATACATCCT AGATGGGCAT CTTATTTGCC AGGACAGGAA 
TACCTTGATG ATTTATATGA GATTGCCAGA TCCAGAGATG ATCCGCTGCT TCTTGATTCC 
TTGGAAAATT TTCTTGCTGC AACAGTAGAT AATTTATTGA GAAAACAGGC AGAAAATGAA 
TACTTGGAGC TTTCTCTTAA AAGAGCAACA TTAAAGAATA ATGAGTTTAG TTCGCAGCTT 
GAACAAGAAA TGGATCAACA TGTTCAAATG CTGGAGGAGA AAATTCGACA AGAAGAACAG 
CAAAAACTGG CTATTCAAAA ACAAGAAGCC TATCAACAGT TAATAATTGC TCAAAAGGAA 
CTGGCTGAAA TGGAGAATAA AGTTAAAATG CTAGAAAAAC AAGTCGAGGA GAAATCAGTT 
AAAACTGTCA TTCCTAATAC AATTTTAGAA GAAATAAAGC TGAAGGAAAA GGAAAATGAA 
ACTCTTCATC TTCGTTTGCT TGAATTTCAA ACACAAATGG CTCTTATGAG AACTGAAGTT 
GCAAAACTTA AGTGTGAATA TGATGAACAA TCTGATCGTC TTCAAAAAGA GCGTGTAACT 
GTTTTAGATT GTGTCCAAGA GCAAGAAAAT TTAACACGAC AATTACATTT GCTACATGAG 
GCAAATAAAA AGTTGCACGA TACTAATGAT GATCTTCGTT CAGCAATGGA GGTCAAGCGC 
TCGAACAGTT ATTTTATTGG CTCTCCAGTA AAACGTACTA CAAGTTTGGT GTTAACTAAG 
TATAGACAGC CGAATTTGCA CAGTTCATTC CGAAGTGATC CTGGACCAGA TTTAAATCTT 
AATGACTTTG ATGACACAGA TATTGAGGAT GCTTCAGGAC GATCAATTAT ACCTTACGAT 
GAAGAAGTCA TTGACAAATC TTCACTCATG GCAGAATTAA TGGAGGCTGA TGACCAAGTT 
CATAATGGCT ATATGTCTGG AAAAAAACCT GTTAGAGTAC TCAGTCTTGA AAACTCTGCT 
TCATCTACCC CTCAAAAACA TGTTGATAGA GAGCGGTTTG TACGTCAACT AGAGGTTTTG 
TGCGAAACAA ATAGAAGATT AGGTGAAAGT AATGATGAAC TTAGAGAAGC ACTAGGAATT 
TTATCAGAGC GACTGCGACC ACCAGATTTG ATAAAAAAAT CCTCTGGTTG TCAATCAGCT 
CCAGTGACTC CTTCAAGAAG AATTTACAGC TCTCCCATCT CCAGCTCTCC TCAAAAGTCG 
GCAGAATCAT CCCACAAAGG TTTTGTTGAC ACAATGAAAA AGTCTGCTGA ATCATCCCAC 
AAAGTTTTTG TTGATACTGC AAAAAAAGAA GTAAAACTTC AATGTTTTAC TCCTTTTAAT 
GAGTCTTCTG TGGATTCTAC TACATTTAAC ACTGATGATG CTACTGAATC TTCAATCTTA 
AAAGGAGAAT ATCATAGAAG AATGGTTGAT GATCGCAGTT ATAATAATAA TAATAGTTTA 
ACAGATTCAA GTAATGAAGC TACAAACATT GAATCTACTG CATATGATGA TGAATCTACT 
GTTTATGATG GGGAATCAAC TGTCTATGAT GGAGAATCAA CTGTCTATGA TGGAGAATCT 
ACTGTTTTAA ACACCTGTTG TTGTGGTAAA ATAATTTTTG AAGACAAAAA TTTGTGCAAA 
GATTGCACTT TTACAGACAC TGAAAAAGGC ACTGGAATTT CAGATACATT TTTGTCAAGT 
GATCAGAGTT TGTTTGATAA TATTCACACT GAAAGTTCTG ACTTGAATAG TTTACATGTC 
CTACCAGCAA AAACTGATAT GTGCACTCAA GTAGATAATA ATCTTAGTTT ATGTGCTATT 
TCTGAAAAAC ATGAAGTTTC AGTTCAGTGT GAATTCCCTC GTGAACTTCC AAGTAAAAAT 
GAACTAAATG TCAATCAGTC TTTTGAACAT AATAAAACAA AATTGCAAAG AAAAGAAGCA 
AAAATGAATA ATCATTTACT AAACAAAACC CAATCAACAG AATATCAGTT TAAAGAAATA 
GATGAGATAG TGCATGATAT AGTTGAATCA AGTCTTGATA AAGCTGATAG TATAAGCTTG 
GATCATATTT ATGAAAAACA CAATAGTAAG GAAGTTAGTC AACAAATTTC TGAAAAACCT 
TACTATATAT CTGACTATAA TTTTTATGAC ATATTTAATG ACCATTCGAT AAATCAAGTA 
CCTGAGTCAA CAATTCTTAC AGACTCTGAT GATAGCAATA ATTATAGTGA TCAAGAAACA 
GGGATTGATA TGAGCATGGT AAAACGTTTC AAACCAGTTG GCGAAGAGAG TAATGAGGAC 
GCTACTTACG AAACTACTAA TGAATACATG ACTAACTACT CTTGGTCTGT AAGCTCTGGA 
TCTGCATTTT TATTGAAAAA AAAAAAAAAA GAGTTAAGCA TACGCTCACA ACGCCAGTTT 
GATGAAGATG TTGATGATTA TAATATGCCA GATGAAGAAC TTGCTCGTTT AGTTAATCAA 
ACAAATGATG ACGATTTTTC AAGCACTAGT GAGGAATTAA TCACTGTAGA AGAAAAAATT 
ATTGAGCCAT GTGAAGCTGC AAAGAATCAT GTCCTGAAGC GTGAGTTTGG GGTTGGTGCA 
GATACTTCAG GTGAATCATT ATCTGCAAAG GAAGGAGATG CAACTGTTCC AGAGAGAATG 
TATAAATTAG TTTTAGTTGG AAATGCAGCT GTTGGAAAAT CAAGTTTTAT TATTCGTTTA 
TGCAAGAATA AATTCTATTC TTCTCTTAAC TCAACTCTTG GTGTTGATTT TCAAATAAAA 
AACCTCCAAT TAAATGGAAA AACAGTAGCT CTGCAGCTCT GGGACACGGC TGGACAAGAA 
AGGTTTCGAA GTATTGCAAA AAGTTACTTT CGAAAAGTTG ATGGCGTTAT ACTCATGTAT 
GATGTAACAT GTGAAAGTTC TTTTATGGAT GTTCGAGATT GGCTGGAATC TATAAAAGAG 
GGATCCTCAA AAACAGTTCC TATCATAATT TGCGGCAATA AAACGGACCT TCGACTTGAT 
GCACGTCGGA AAGGTATGCC AGTTGTCAGT CAAGAACAAG GTCAAAGGTT AGCAAATGAC 
ATTGGAGCTC TTTTTATTGA AACCAGCGCA AAAGAAGGAA CAAACATCAC TAACACATGC 
ATAGAATTAA CCAAACAATT AATTCAAAAT GATGAAAAAC GATTGTCTGC CACAGGAATG 
CAACTAAATA AACAGCCGAG TCAAACAAAA AAAAATGGTT GCTGTCGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100214119 ( XM_012705293.1 ) cDNA ORF clone, Hydra vulgaris -> XP_012560747.1 Hydra vulgaris ras and EF-hand domain-containing protein homolog (LOC100214119), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHc18049
Clone ID Related Accession (Same CDS sequence)	XM_012705293.1
Accession Version	XM_012705293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433606400000
Organism	Hydra vulgaris
Product	uncharacterized protein LOC100214119 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004166982.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hydra vulgaris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGAATG TTGATGAAAA TGCTTTAATG AAACGTGAAC TTCTGGATGC AGAACTAGAT 
TCAAGCGGCT TTATTGATGA GGTTGAACTG GCTGCAGTAG TTGATCTAAA ACCATATGAA 
CTGAAACAAA TATTTGCAAG ACTAGACACT GATAAAGATG GAAAGATATC AATCACAGAC 
TTTATGGAAA ACTACACGCT TTTCCAATCG TATGCGGACG TTAAAAACAC GTCAATGCAA 
CCCGAAATTA AAACAAATAG TACTGAGTAT TTTGAAAATG GAAAAATAGC CTTAAAAGAA 
AACTCAAATG ATAGTGGATT TAATTCTACT AATACTGCTA TGAAGATACC TGATGCTTCA 
TCTGACGATA CGTTAAAAGT ACCTGATGAT ATGATGAGGA TACCTGATGC TTCAATGAGT 
TTAAAAAACT CATCATTAAA AGTATCAGAT GCTGCATTCA ATTTAAAAAA TAAACCATTG 
ATTATTGATT TAAAGACCGA GGCGAAAAAT CAACTAGGAC AGGAATACCT TGATGATTTA 
TATGAGATTG CCAGATCCAG AGATGATCCG CTGCTTCTTG ATTCCTTGGA AAATTTTCTT 
GCTGCAACAG TAGATAATTT ATTGAGAAAA CAGGCAGAAA ATGAATACTT GGAGCTTTCT 
CTTAAAAGAG CAACATTAAA GAATAATGAG TTTAGTTCGC AGCTTGAACA AGAAATGGAT 
CAACATGTTC AAATGCTGGA GGAGAAAATT CGACAAGAAG AACAGCAAAA ACTGGCTATT 
CAAAAACAAG AAGCCTATCA ACAGTTAATA ATTGCTCAAA AGGAACTGGC TGAAATGGAG 
AATAAAGTTA AAATGCTAGA AAAACAAGTC GAGGAGAAAT CAGTTAAAAC TGTCATTCCT 
AATACAATTT TAGAAGAAAT AAAGCTGAAG GAAAAGGAAA ATGAAACTCT TCATCTTCGT 
TTGCTTGAAT TTCAAACACA AATGGCTCTT ATGAGAACTG AAGTTGCAAA ACTTAAGTGT 
GAATATGATG AACAATCTGA TCGTCTTCAA AAAGAGCGTG TAACTGTTTT AGATTGTGTC 
CAAGAGCAAG AAAATTTAAC ACGACAATTA CATTTGCTAC ATGAGGCAAA TAAAAAGTTG 
CACGATACTA ATGATGATCT TCGTTCAGCA ATGGAGGTCA AGCGCTCGAA CAGTTATTTT 
ATTGGCTCTC CAGTAAAACG TACTACAAGT TTGGTGTTAA CTAAGTATAG ACAGCCGAAT 
TTGCACAGTT CATTCCGAAG TGATCCTGGA CCAGATTTAA ATCTTAATGA CTTTGATGAC 
ACAGATATTG AGGATGCTTC AGGACGATCA ATTATACCTT ACGATGAAGA AGTCATTGAC 
AAATCTTCAC TCATGGCAGA ATTAATGGAG GCTGATGACC AAGTTCATAA TGGCTATATG 
TCTGGAAAAA AACCTGTTAG AGTACTCAGT CTTGAAAACT CTGCTTCATC TACCCCTCAA 
AAACATGTTG ATAGAGAGCG GTTTGTACGT CAACTAGAGG TTTTGTGCGA AACAAATAGA 
AGATTAGGTG AAAGTAATGA TGAACTTAGA GAAGCACTAG GAATTTTATC AGAGCGACTG 
CGACCACCAG ATTTGATAAA AAAATCCTCT GGTTGTCAAT CAGCTCCAGT GACTCCTTCA 
AGAAGAATTT ACAGCTCTCC CATCTCCAGC TCTCCTCAAA AGTCGGCAGA ATCATCCCAC 
AAAGGTTTTG TTGACACAAT GAAAAAGTCT GCTGAATCAT CCCACAAAGT TTTTGTTGAT 
ACTGCAAAAA AAGAAGTAAA ACTTCAATGT TTTACTCCTT TTAATGAGTC TTCTGTGGAT 
TCTACTACAT TTAACACTGA TGATGCTACT GAATCTTCAA TCTTAAAAGG AGAATATCAT 
AGAAGAATGG TTGATGATCG CAGTTATAAT AATAATAATA GTTTAACAGA TTCAAGTAAT 
GAAGCTACAA ACATTGAATC TACTGCATAT GATGATGAAT CTACTGTTTA TGATGGGGAA 
TCAACTGTCT ATGATGGAGA ATCAACTGTC TATGATGGAG AATCTACTGT TTTAAACACC 
TGTTGTTGTG GTAAAATAAT TTTTGAAGAC AAAAATTTGT GCAAAGATTG CACTTTTACA 
GACACTGAAA AAGGCACTGG AATTTCAGAT ACATTTTTGT CAAGTGATCA GAGTTTGTTT 
GATAATATTC ACACTGAAAG TTCTGACTTG AATAGTTTAC ATGTCCTACC AGCAAAAACT 
GATATGTGCA CTCAAGTAGA TAATAATCTT AGTTTATGTG CTATTTCTGA AAAACATGAA 
GTTTCAGTTC AGTGTGAATT CCCTCGTGAA CTTCCAAGTA AAAATGAACT AAATGTCAAT 
CAGTCTTTTG AACATAATAA AACAAAATTG CAAAGAAAAG AAGCAAAAAT GAATAATCAT 
TTACTAAACA AAACCCAATC AACAGAATAT CAGTTTAAAG AAATAGATGA GATAGTGCAT 
GATATAGTTG AATCAAGTCT TGATAAAGCT GATAGTATAA GCTTGGATCA TATTTATGAA 
AAACACAATA GTAAGGAAGT TAGTCAACAA ATTTCTGAAA AACCTTACTA TATATCTGAC 
TATAATTTTT ATGACATATT TAATGACCAT TCGATAAATC AAGTACCTGA GTCAACAATT 
CTTACAGACT CTGATGATAG CAATAATTAT AGTGATCAAG AAACAGGGAT TGATATGAGC 
ATGGTAAAAC GTTTCAAACC AGTTGGCGAA GAGAGTAATG AGGACGCTAC TTACGAAACT 
ACTAATGAAT ACATGACTAA CTACTCTTGG TCTGTAAGCT CTGGATCTGC ATTTTTATTG 
AAAAAAAAAA AAAAAGAGTT AAGCATACGC TCACAACGCC AGTTTGATGA AGATGTTGAT 
GATTATAATA TGCCAGATGA AGAACTTGCT CGTTTAGTTA ATCAAACAAA TGATGACGAT 
TTTTCAAGCA CTAGTGAGGA ATTAATCACT GTAGAAGAAA AAATTATTGA GCCATGTGAA 
GCTGCAAAGA ATCATGTCCT GAAGCGTGAG TTTGGGGTTG GTGCAGATAC TTCAGGTGAA 
TCATTATCTG CAAAGGAAGG AGATGCAACT GTTCCAGAGA GAATGTATAA ATTAGTTTTA 
GTTGGAAATG CAGCTGTTGG AAAATCAAGT TTTATTATTC GTTTATGCAA GAATAAATTC 
TATTCTTCTC TTAACTCAAC TCTTGGTGTT GATTTTCAAA TAAAAAACCT CCAATTAAAT 
GGAAAAACAG TAGCTCTGCA GCTCTGGGAC ACGGCTGGAC AAGAAAGGTT TCGAAGTATT 
GCAAAAAGTT ACTTTCGAAA AGTTGATGGC GTTATACTCA TGTATGATGT AACATGTGAA 
AGTTCTTTTA TGGATGTTCG AGATTGGCTG GAATCTATAA AAGAGGGATC CTCAAAAACA 
GTTCCTATCA TAATTTGCGG CAATAAAACG GACCTTCGAC TTGATGCACG TCGGAAAGGT 
ATGCCAGTTG TCAGTCAAGA ACAAGGTCAA AGGTTAGCAA ATGACATTGG AGCTCTTTTT 
ATTGAAACCA GCGCAAAAGA AGGAACAAAC ATCACTAACA CATGCATAGA ATTAACCAAA 
CAATTAATTC AAAATGATGA AAAACGATTG TCTGCCACAG GAATGCAACT AAATAAACAG 
CCGAGTCAAA CAAAAAAAAA TGGTTGCTGT CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012560747.1
CDS	292..4047
Translation	MSNVDENALMKRELLDAELDSSGFIDEVELAAVVDLKPYELKQIFARLDTDKDGKISITDFMENYTLFQSYADVKNTSMQPEIKTNSTEYFENGKIALKENSNDSGFNSTNTAMKIPDASSDDTLKVPDDMMRIPDASMSLKNSSLKVSDAAFNLKNKPLIIDLKTEAKNQLGQEYLDDLYEIARSRDDPLLLDSLENFLAATVDNLLRKQAENEYLELSLKRATLKNNEFSSQLEQEMDQHVQMLEEKIRQEEQQKLAIQKQEAYQQLIIAQKELAEMENKVKMLEKQVEEKSVKTVIPNTILEEIKLKEKENETLHLRLLEFQTQMALMRTEVAKLKCEYDEQSDRLQKERVTVLDCVQEQENLTRQLHLLHEANKKLHDTNDDLRSAMEVKRSNSYFIGSPVKRTTSLVLTKYRQPNLHSSFRSDPGPDLNLNDFDDTDIEDASGRSIIPYDEEVIDKSSLMAELMEADDQVHNGYMSGKKPVRVLSLENSASSTPQKHVDRERFVRQLEVLCETNRRLGESNDELREALGILSERLRPPDLIKKSSGCQSAPVTPSRRIYSSPISSSPQKSAESSHKGFVDTMKKSAESSHKVFVDTAKKEVKLQCFTPFNESSVDSTTFNTDDATESSILKGEYHRRMVDDRSYNNNNSLTDSSNEATNIESTAYDDESTVYDGESTVYDGESTVYDGESTVLNTCCCGKIIFEDKNLCKDCTFTDTEKGTGISDTFLSSDQSLFDNIHTESSDLNSLHVLPAKTDMCTQVDNNLSLCAISEKHEVSVQCEFPRELPSKNELNVNQSFEHNKTKLQRKEAKMNNHLLNKTQSTEYQFKEIDEIVHDIVESSLDKADSISLDHIYEKHNSKEVSQQISEKPYYISDYNFYDIFNDHSINQVPESTILTDSDDSNNYSDQETGIDMSMVKRFKPVGEESNEDATYETTNEYMTNYSWSVSSGSAFLLKKKKKELSIRSQRQFDEDVDDYNMPDEELARLVNQTNDDDFSSTSEELITVEEKIIEPCEAAKNHVLKREFGVGADTSGESLSAKEGDATVPERMYKLVLVGNAAVGKSSFIIRLCKNKFYSSLNSTLGVDFQIKNLQLNGKTVALQLWDTAGQERFRSIAKSYFRKVDGVILMYDVTCESSFMDVRDWLESIKEGSSKTVPIIICGNKTDLRLDARRKGMPVVSQEQGQRLANDIGALFIETSAKEGTNITNTCIELTKQLIQNDEKRLSATGMQLNKQPSQTKKNGCCR

Target ORF information:

RefSeq Version	XM_012705293.1
Organism	Hydra vulgaris
Definition	Hydra vulgaris ras and EF-hand domain-containing protein homolog (LOC100214119), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012705293.1

ORF Insert Sequence:

ATGTCGAATG TTGATGAAAA TGCTTTAATG AAACGTGAAC TTCTGGATGC AGAACTAGAT 
TCAAGCGGCT TTATTGATGA GGTTGAACTG GCTGCAGTAG TTGATCTAAA ACCATATGAA 
CTGAAACAAA TATTTGCAAG ACTAGACACT GATAAAGATG GAAAGATATC AATCACAGAC 
TTTATGGAAA ACTACACGCT TTTCCAATCG TATGCGGACG TTAAAAACAC GTCAATGCAA 
CCCGAAATTA AAACAAATAG TACTGAGTAT TTTGAAAATG GAAAAATAGC CTTAAAAGAA 
AACTCAAATG ATAGTGGATT TAATTCTACT AATACTGCTA TGAAGATACC TGATGCTTCA 
TCTGACGATA CGTTAAAAGT ACCTGATGAT ATGATGAGGA TACCTGATGC TTCAATGAGT 
TTAAAAAACT CATCATTAAA AGTATCAGAT GCTGCATTCA ATTTAAAAAA TAAACCATTG 
ATTATTGATT TAAAGACCGA GGCGAAAAAT CAACTAGGAC AGGAATACCT TGATGATTTA 
TATGAGATTG CCAGATCCAG AGATGATCCG CTGCTTCTTG ATTCCTTGGA AAATTTTCTT 
GCTGCAACAG TAGATAATTT ATTGAGAAAA CAGGCAGAAA ATGAATACTT GGAGCTTTCT 
CTTAAAAGAG CAACATTAAA GAATAATGAG TTTAGTTCGC AGCTTGAACA AGAAATGGAT 
CAACATGTTC AAATGCTGGA GGAGAAAATT CGACAAGAAG AACAGCAAAA ACTGGCTATT 
CAAAAACAAG AAGCCTATCA ACAGTTAATA ATTGCTCAAA AGGAACTGGC TGAAATGGAG 
AATAAAGTTA AAATGCTAGA AAAACAAGTC GAGGAGAAAT CAGTTAAAAC TGTCATTCCT 
AATACAATTT TAGAAGAAAT AAAGCTGAAG GAAAAGGAAA ATGAAACTCT TCATCTTCGT 
TTGCTTGAAT TTCAAACACA AATGGCTCTT ATGAGAACTG AAGTTGCAAA ACTTAAGTGT 
GAATATGATG AACAATCTGA TCGTCTTCAA AAAGAGCGTG TAACTGTTTT AGATTGTGTC 
CAAGAGCAAG AAAATTTAAC ACGACAATTA CATTTGCTAC ATGAGGCAAA TAAAAAGTTG 
CACGATACTA ATGATGATCT TCGTTCAGCA ATGGAGGTCA AGCGCTCGAA CAGTTATTTT 
ATTGGCTCTC CAGTAAAACG TACTACAAGT TTGGTGTTAA CTAAGTATAG ACAGCCGAAT 
TTGCACAGTT CATTCCGAAG TGATCCTGGA CCAGATTTAA ATCTTAATGA CTTTGATGAC 
ACAGATATTG AGGATGCTTC AGGACGATCA ATTATACCTT ACGATGAAGA AGTCATTGAC 
AAATCTTCAC TCATGGCAGA ATTAATGGAG GCTGATGACC AAGTTCATAA TGGCTATATG 
TCTGGAAAAA AACCTGTTAG AGTACTCAGT CTTGAAAACT CTGCTTCATC TACCCCTCAA 
AAACATGTTG ATAGAGAGCG GTTTGTACGT CAACTAGAGG TTTTGTGCGA AACAAATAGA 
AGATTAGGTG AAAGTAATGA TGAACTTAGA GAAGCACTAG GAATTTTATC AGAGCGACTG 
CGACCACCAG ATTTGATAAA AAAATCCTCT GGTTGTCAAT CAGCTCCAGT GACTCCTTCA 
AGAAGAATTT ACAGCTCTCC CATCTCCAGC TCTCCTCAAA AGTCGGCAGA ATCATCCCAC 
AAAGGTTTTG TTGACACAAT GAAAAAGTCT GCTGAATCAT CCCACAAAGT TTTTGTTGAT 
ACTGCAAAAA AAGAAGTAAA ACTTCAATGT TTTACTCCTT TTAATGAGTC TTCTGTGGAT 
TCTACTACAT TTAACACTGA TGATGCTACT GAATCTTCAA TCTTAAAAGG AGAATATCAT 
AGAAGAATGG TTGATGATCG CAGTTATAAT AATAATAATA GTTTAACAGA TTCAAGTAAT 
GAAGCTACAA ACATTGAATC TACTGCATAT GATGATGAAT CTACTGTTTA TGATGGGGAA 
TCAACTGTCT ATGATGGAGA ATCAACTGTC TATGATGGAG AATCTACTGT TTTAAACACC 
TGTTGTTGTG GTAAAATAAT TTTTGAAGAC AAAAATTTGT GCAAAGATTG CACTTTTACA 
GACACTGAAA AAGGCACTGG AATTTCAGAT ACATTTTTGT CAAGTGATCA GAGTTTGTTT 
GATAATATTC ACACTGAAAG TTCTGACTTG AATAGTTTAC ATGTCCTACC AGCAAAAACT 
GATATGTGCA CTCAAGTAGA TAATAATCTT AGTTTATGTG CTATTTCTGA AAAACATGAA 
GTTTCAGTTC AGTGTGAATT CCCTCGTGAA CTTCCAAGTA AAAATGAACT AAATGTCAAT 
CAGTCTTTTG AACATAATAA AACAAAATTG CAAAGAAAAG AAGCAAAAAT GAATAATCAT 
TTACTAAACA AAACCCAATC AACAGAATAT CAGTTTAAAG AAATAGATGA GATAGTGCAT 
GATATAGTTG AATCAAGTCT TGATAAAGCT GATAGTATAA GCTTGGATCA TATTTATGAA 
AAACACAATA GTAAGGAAGT TAGTCAACAA ATTTCTGAAA AACCTTACTA TATATCTGAC 
TATAATTTTT ATGACATATT TAATGACCAT TCGATAAATC AAGTACCTGA GTCAACAATT 
CTTACAGACT CTGATGATAG CAATAATTAT AGTGATCAAG AAACAGGGAT TGATATGAGC 
ATGGTAAAAC GTTTCAAACC AGTTGGCGAA GAGAGTAATG AGGACGCTAC TTACGAAACT 
ACTAATGAAT ACATGACTAA CTACTCTTGG TCTGTAAGCT CTGGATCTGC ATTTTTATTG 
AAAAAAAAAA AAAAAGAGTT AAGCATACGC TCACAACGCC AGTTTGATGA AGATGTTGAT 
GATTATAATA TGCCAGATGA AGAACTTGCT CGTTTAGTTA ATCAAACAAA TGATGACGAT 
TTTTCAAGCA CTAGTGAGGA ATTAATCACT GTAGAAGAAA AAATTATTGA GCCATGTGAA 
GCTGCAAAGA ATCATGTCCT GAAGCGTGAG TTTGGGGTTG GTGCAGATAC TTCAGGTGAA 
TCATTATCTG CAAAGGAAGG AGATGCAACT GTTCCAGAGA GAATGTATAA ATTAGTTTTA 
GTTGGAAATG CAGCTGTTGG AAAATCAAGT TTTATTATTC GTTTATGCAA GAATAAATTC 
TATTCTTCTC TTAACTCAAC TCTTGGTGTT GATTTTCAAA TAAAAAACCT CCAATTAAAT 
GGAAAAACAG TAGCTCTGCA GCTCTGGGAC ACGGCTGGAC AAGAAAGGTT TCGAAGTATT 
GCAAAAAGTT ACTTTCGAAA AGTTGATGGC GTTATACTCA TGTATGATGT AACATGTGAA 
AGTTCTTTTA TGGATGTTCG AGATTGGCTG GAATCTATAA AAGAGGGATC CTCAAAAACA 
GTTCCTATCA TAATTTGCGG CAATAAAACG GACCTTCGAC TTGATGCACG TCGGAAAGGT 
ATGCCAGTTG TCAGTCAAGA ACAAGGTCAA AGGTTAGCAA ATGACATTGG AGCTCTTTTT 
ATTGAAACCA GCGCAAAAGA AGGAACAAAC ATCACTAACA CATGCATAGA ATTAACCAAA 
CAATTAATTC AAAATGATGA AAAACGATTG TCTGCCACAG GAATGCAACT AAATAAACAG 
CCGAGTCAAA CAAAAAAAAA TGGTTGCTGT CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100214119 ( XM_012705299.1 ) cDNA ORF clone, Hydra vulgaris -> XP_012560753.1 Hydra vulgaris ras and EF-hand domain-containing protein homolog (LOC100214119), transcript variant X3, mRNA.

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CloneID	OHc18050
Clone ID Related Accession (Same CDS sequence)	XM_012705299.1
Accession Version	XM_012705299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433606400000
Organism	Hydra vulgaris
Product	uncharacterized protein LOC100214119 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004166982.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hydra vulgaris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTCAG AATTTGTTGA AGTAACGTTT TCTGTGCCAA GAAATAGTTT TTTTCAATAT 
TCACCCAATG TTAATGGAAA CACACATGTT GTTCCTGATA ATAACTACAA TAGTTCTCTA 
AACAATCGTT CTTATTGCTA TGTACAAGAT CATCTTGCAG TAGAAAAAGA ATCATTCTAT 
ACCAATTTTT ATGATTGTAC CAATACAACG CATGCAAGTA AAGAATCTTG TGACCTCAAT 
AACTTATCAA CCAAAAATCT TTTCCAAAAA AATTCTGACA CTCGAACTTC TTGCACTATG 
TACGAATCAA TAAAACAACC ACACAATTCT TCAATACAAA TAAAATCACT CGGTGTCACA 
CCGTGGCGCC TTTTTCCTCA AAATGACAGC ATCTTTACTA GTTCGCCTCT CTGTTTCAAT 
AAAGATAATG TATCTCAAAT TAAACCTAAA ATCTTACTTT CTCAAAAAGA GGTGCCAACT 
TTTGAAGAGT TTTTAAATAA GATACATCCT AGATGGGCAT CTTATTTGCC AGGACAGGAA 
TACCTTGATG ATTTATATGA GATTGCCAGA TCCAGAGATG ATCCGCTGCT TCTTGATTCC 
TTGGAAAATT TTCTTGCTGC AACAGTAGAT AATTTATTGA GAAAACAGGC AGAAAATGAA 
TACTTGGAGC TTTCTCTTAA AAGAGCAACA TTAAAGAATA ATGAGTTTAG TTCGCAGCTT 
GAACAAGAAA TGGATCAACA TGTTCAAATG CTGGAGGAGA AAATTCGACA AGAAGAACAG 
CAAAAACTGG CTATTCAAAA ACAAGAAGCC TATCAACAGT TAATAATTGC TCAAAAGGAA 
CTGGCTGAAA TGGAGAATAA AGTTAAAATG CTAGAAAAAC AAGTCGAGGA GAAATCAGTT 
AAAACTGTCA TTCCTAATAC AATTTTAGAA GAAATAAAGC TGAAGGAAAA GGAAAATGAA 
ACTCTTCATC TTCGTTTGCT TGAATTTCAA ACACAAATGG CTCTTATGAG AACTGAAGTT 
GCAAAACTTA AGTGTGAATA TGATGAACAA TCTGATCGTC TTCAAAAAGA GCGTGTAACT 
GTTTTAGATT GTGTCCAAGA GCAAGAAAAT TTAACACGAC AATTACATTT GCTACATGAG 
GCAAATAAAA AGTTGCACGA TACTAATGAT GATCTTCGTT CAGCAATGGA GGTCAAGCGC 
TCGAACAGTT ATTTTATTGG CTCTCCAGTA AAACGTACTA CAAGTTTGGT GTTAACTAAG 
TATAGACAGC CGAATTTGCA CAGTTCATTC CGAAGTGATC CTGGACCAGA TTTAAATCTT 
AATGACTTTG ATGACACAGA TATTGAGGAT GCTTCAGGAC GATCAATTAT ACCTTACGAT 
GAAGAAGTCA TTGACAAATC TTCACTCATG GCAGAATTAA TGGAGGCTGA TGACCAAGTT 
CATAATGGCT ATATGTCTGG AAAAAAACCT GTTAGAGTAC TCAGTCTTGA AAACTCTGCT 
TCATCTACCC CTCAAAAACA TGTTGATAGA GAGCGGTTTG TACGTCAACT AGAGGTTTTG 
TGCGAAACAA ATAGAAGATT AGGTGAAAGT AATGATGAAC TTAGAGAAGC ACTAGGAATT 
TTATCAGAGC GACTGCGACC ACCAGATTTG ATAAAAAAAT CCTCTGGTTG TCAATCAGCT 
CCAGTGACTC CTTCAAGAAG AATTTACAGC TCTCCCATCT CCAGCTCTCC TCAAAAGTCG 
GCAGAATCAT CCCACAAAGG TTTTGTTGAC ACAATGAAAA AGTCTGCTGA ATCATCCCAC 
AAAGTTTTTG TTGATACTGC AAAAAAAGAA GTAAAACTTC AATGTTTTAC TCCTTTTAAT 
GAGTCTTCTG TGGATTCTAC TACATTTAAC ACTGATGATG CTACTGAATC TTCAATCTTA 
AAAGGAGAAT ATCATAGAAG AATGGTTGAT GATCGCAGTT ATAATAATAA TAATAGTTTA 
ACAGATTCAA GTAATGAAGC TACAAACATT GAATCTACTG CATATGATGA TGAATCTACT 
GTTTATGATG GGGAATCAAC TGTCTATGAT GGAGAATCAA CTGTCTATGA TGGAGAATCT 
ACTGTTTTAA ACACCTGTTG TTGTGGTAAA ATAATTTTTG AAGACAAAAA TTTGTGCAAA 
GATTGCACTT TTACAGACAC TGAAAAAGGC ACTGGAATTT CAGATACATT TTTGTCAAGT 
GATCAGAGTT TGTTTGATAA TATTCACACT GAAAGTTCTG ACTTGAATAG TTTACATGTC 
CTACCAGCAA AAACTGATAT GTGCACTCAA GTAGATAATA ATCTTAGTTT ATGTGCTATT 
TCTGAAAAAC ATGAAGTTTC AGTTCAGTGT GAATTCCCTC GTGAACTTCC AAGTAAAAAT 
GAACTAAATG TCAATCAGTC TTTTGAACAT AATAAAACAA AATTGCAAAG AAAAGAAGCA 
AAAATGAATA ATCATTTACT AAACAAAACC CAATCAACAG AATATCAGTT TAAAGAAATA 
GATGAGATAG TGCATGATAT AGTTGAATCA AGTCTTGATA AAGCTGATAG TATAAGCTTG 
GATCATATTT ATGAAAAACA CAATAGTAAG GAAGTTAGTC AACAAATTTC TGAAAAACCT 
TACTATATAT CTGACTATAA TTTTTATGAC ATATTTAATG ACCATTCGAT AAATCAAGTA 
CCTGAGTCAA CAATTCTTAC AGACTCTGAT GATAGCAATA ATTATAGTGA TCAAGAAACA 
GGGATTGATA TGAGCATGGT AAAACGTTTC AAACCAGTTG GCGAAGAGAG TAATGAGTTA 
AGCATACGCT CACAACGCCA GTTTGATGAA GATGTTGATG ATTATAATAT GCCAGATGAA 
GAACTTGCTC GTTTAGTTAA TCAAACAAAT GATGACGATT TTTCAAGCAC TAGTGAGGAA 
TTAATCACTG TAGAAGAAAA AATTATTGAG CCATGTGAAG CTGCAAAGAA TCATGTCCTG 
AAGCGTGAGT TTGGGGTTGG TGCAGATACT TCAGGTGAAT CATTATCTGC AAAGGAAGGA 
GATGCAACTG TTCCAGAGAG AATGTATAAA TTAGTTTTAG TTGGAAATGC AGCTGTTGGA 
AAATCAAGTT TTATTATTCG TTTATGCAAG AATAAATTCT ATTCTTCTCT TAACTCAACT 
CTTGGTGTTG ATTTTCAAAT AAAAAACCTC CAATTAAATG GAAAAACAGT AGCTCTGCAG 
CTCTGGGACA CGGCTGGACA AGAAAGGTTT CGAAGTATTG CAAAAAGTTA CTTTCGAAAA 
GTTGATGGCG TTATACTCAT GTATGATGTA ACATGTGAAA GTTCTTTTAT GGATGTTCGA 
GATTGGCTGG AATCTATAAA AGAGGGATCC TCAAAAACAG TTCCTATCAT AATTTGCGGC 
AATAAAACGG ACCTTCGACT TGATGCACGT CGGAAAGGTA TGCCAGTTGT CAGTCAAGAA 
CAAGGTCAAA GGTTAGCAAA TGACATTGGA GCTCTTTTTA TTGAAACCAG CGCAAAAGAA 
GGAACAAACA TCACTAACAC ATGCATAGAA TTAACCAAAC AATTAATTCA AAATGATGAA 
AAACGATTGT CTGCCACAGG AATGCAACTA AATAAACAGC CGAGTCAAAC AAAAAAAAAT 
GGTTGCTGTC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012560753.1
CDS	142..3816
Translation	MNSEFVEVTFSVPRNSFFQYSPNVNGNTHVVPDNNYNSSLNNRSYCYVQDHLAVEKESFYTNFYDCTNTTHASKESCDLNNLSTKNLFQKNSDTRTSCTMYESIKQPHNSSIQIKSLGVTPWRLFPQNDSIFTSSPLCFNKDNVSQIKPKILLSQKEVPTFEEFLNKIHPRWASYLPGQEYLDDLYEIARSRDDPLLLDSLENFLAATVDNLLRKQAENEYLELSLKRATLKNNEFSSQLEQEMDQHVQMLEEKIRQEEQQKLAIQKQEAYQQLIIAQKELAEMENKVKMLEKQVEEKSVKTVIPNTILEEIKLKEKENETLHLRLLEFQTQMALMRTEVAKLKCEYDEQSDRLQKERVTVLDCVQEQENLTRQLHLLHEANKKLHDTNDDLRSAMEVKRSNSYFIGSPVKRTTSLVLTKYRQPNLHSSFRSDPGPDLNLNDFDDTDIEDASGRSIIPYDEEVIDKSSLMAELMEADDQVHNGYMSGKKPVRVLSLENSASSTPQKHVDRERFVRQLEVLCETNRRLGESNDELREALGILSERLRPPDLIKKSSGCQSAPVTPSRRIYSSPISSSPQKSAESSHKGFVDTMKKSAESSHKVFVDTAKKEVKLQCFTPFNESSVDSTTFNTDDATESSILKGEYHRRMVDDRSYNNNNSLTDSSNEATNIESTAYDDESTVYDGESTVYDGESTVYDGESTVLNTCCCGKIIFEDKNLCKDCTFTDTEKGTGISDTFLSSDQSLFDNIHTESSDLNSLHVLPAKTDMCTQVDNNLSLCAISEKHEVSVQCEFPRELPSKNELNVNQSFEHNKTKLQRKEAKMNNHLLNKTQSTEYQFKEIDEIVHDIVESSLDKADSISLDHIYEKHNSKEVSQQISEKPYYISDYNFYDIFNDHSINQVPESTILTDSDDSNNYSDQETGIDMSMVKRFKPVGEESNELSIRSQRQFDEDVDDYNMPDEELARLVNQTNDDDFSSTSEELITVEEKIIEPCEAAKNHVLKREFGVGADTSGESLSAKEGDATVPERMYKLVLVGNAAVGKSSFIIRLCKNKFYSSLNSTLGVDFQIKNLQLNGKTVALQLWDTAGQERFRSIAKSYFRKVDGVILMYDVTCESSFMDVRDWLESIKEGSSKTVPIIICGNKTDLRLDARRKGMPVVSQEQGQRLANDIGALFIETSAKEGTNITNTCIELTKQLIQNDEKRLSATGMQLNKQPSQTKKNGCCR

Target ORF information:

RefSeq Version	XM_012705299.1
Organism	Hydra vulgaris
Definition	Hydra vulgaris ras and EF-hand domain-containing protein homolog (LOC100214119), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012705299.1

ORF Insert Sequence:

ATGAACTCAG AATTTGTTGA AGTAACGTTT TCTGTGCCAA GAAATAGTTT TTTTCAATAT 
TCACCCAATG TTAATGGAAA CACACATGTT GTTCCTGATA ATAACTACAA TAGTTCTCTA 
AACAATCGTT CTTATTGCTA TGTACAAGAT CATCTTGCAG TAGAAAAAGA ATCATTCTAT 
ACCAATTTTT ATGATTGTAC CAATACAACG CATGCAAGTA AAGAATCTTG TGACCTCAAT 
AACTTATCAA CCAAAAATCT TTTCCAAAAA AATTCTGACA CTCGAACTTC TTGCACTATG 
TACGAATCAA TAAAACAACC ACACAATTCT TCAATACAAA TAAAATCACT CGGTGTCACA 
CCGTGGCGCC TTTTTCCTCA AAATGACAGC ATCTTTACTA GTTCGCCTCT CTGTTTCAAT 
AAAGATAATG TATCTCAAAT TAAACCTAAA ATCTTACTTT CTCAAAAAGA GGTGCCAACT 
TTTGAAGAGT TTTTAAATAA GATACATCCT AGATGGGCAT CTTATTTGCC AGGACAGGAA 
TACCTTGATG ATTTATATGA GATTGCCAGA TCCAGAGATG ATCCGCTGCT TCTTGATTCC 
TTGGAAAATT TTCTTGCTGC AACAGTAGAT AATTTATTGA GAAAACAGGC AGAAAATGAA 
TACTTGGAGC TTTCTCTTAA AAGAGCAACA TTAAAGAATA ATGAGTTTAG TTCGCAGCTT 
GAACAAGAAA TGGATCAACA TGTTCAAATG CTGGAGGAGA AAATTCGACA AGAAGAACAG 
CAAAAACTGG CTATTCAAAA ACAAGAAGCC TATCAACAGT TAATAATTGC TCAAAAGGAA 
CTGGCTGAAA TGGAGAATAA AGTTAAAATG CTAGAAAAAC AAGTCGAGGA GAAATCAGTT 
AAAACTGTCA TTCCTAATAC AATTTTAGAA GAAATAAAGC TGAAGGAAAA GGAAAATGAA 
ACTCTTCATC TTCGTTTGCT TGAATTTCAA ACACAAATGG CTCTTATGAG AACTGAAGTT 
GCAAAACTTA AGTGTGAATA TGATGAACAA TCTGATCGTC TTCAAAAAGA GCGTGTAACT 
GTTTTAGATT GTGTCCAAGA GCAAGAAAAT TTAACACGAC AATTACATTT GCTACATGAG 
GCAAATAAAA AGTTGCACGA TACTAATGAT GATCTTCGTT CAGCAATGGA GGTCAAGCGC 
TCGAACAGTT ATTTTATTGG CTCTCCAGTA AAACGTACTA CAAGTTTGGT GTTAACTAAG 
TATAGACAGC CGAATTTGCA CAGTTCATTC CGAAGTGATC CTGGACCAGA TTTAAATCTT 
AATGACTTTG ATGACACAGA TATTGAGGAT GCTTCAGGAC GATCAATTAT ACCTTACGAT 
GAAGAAGTCA TTGACAAATC TTCACTCATG GCAGAATTAA TGGAGGCTGA TGACCAAGTT 
CATAATGGCT ATATGTCTGG AAAAAAACCT GTTAGAGTAC TCAGTCTTGA AAACTCTGCT 
TCATCTACCC CTCAAAAACA TGTTGATAGA GAGCGGTTTG TACGTCAACT AGAGGTTTTG 
TGCGAAACAA ATAGAAGATT AGGTGAAAGT AATGATGAAC TTAGAGAAGC ACTAGGAATT 
TTATCAGAGC GACTGCGACC ACCAGATTTG ATAAAAAAAT CCTCTGGTTG TCAATCAGCT 
CCAGTGACTC CTTCAAGAAG AATTTACAGC TCTCCCATCT CCAGCTCTCC TCAAAAGTCG 
GCAGAATCAT CCCACAAAGG TTTTGTTGAC ACAATGAAAA AGTCTGCTGA ATCATCCCAC 
AAAGTTTTTG TTGATACTGC AAAAAAAGAA GTAAAACTTC AATGTTTTAC TCCTTTTAAT 
GAGTCTTCTG TGGATTCTAC TACATTTAAC ACTGATGATG CTACTGAATC TTCAATCTTA 
AAAGGAGAAT ATCATAGAAG AATGGTTGAT GATCGCAGTT ATAATAATAA TAATAGTTTA 
ACAGATTCAA GTAATGAAGC TACAAACATT GAATCTACTG CATATGATGA TGAATCTACT 
GTTTATGATG GGGAATCAAC TGTCTATGAT GGAGAATCAA CTGTCTATGA TGGAGAATCT 
ACTGTTTTAA ACACCTGTTG TTGTGGTAAA ATAATTTTTG AAGACAAAAA TTTGTGCAAA 
GATTGCACTT TTACAGACAC TGAAAAAGGC ACTGGAATTT CAGATACATT TTTGTCAAGT 
GATCAGAGTT TGTTTGATAA TATTCACACT GAAAGTTCTG ACTTGAATAG TTTACATGTC 
CTACCAGCAA AAACTGATAT GTGCACTCAA GTAGATAATA ATCTTAGTTT ATGTGCTATT 
TCTGAAAAAC ATGAAGTTTC AGTTCAGTGT GAATTCCCTC GTGAACTTCC AAGTAAAAAT 
GAACTAAATG TCAATCAGTC TTTTGAACAT AATAAAACAA AATTGCAAAG AAAAGAAGCA 
AAAATGAATA ATCATTTACT AAACAAAACC CAATCAACAG AATATCAGTT TAAAGAAATA 
GATGAGATAG TGCATGATAT AGTTGAATCA AGTCTTGATA AAGCTGATAG TATAAGCTTG 
GATCATATTT ATGAAAAACA CAATAGTAAG GAAGTTAGTC AACAAATTTC TGAAAAACCT 
TACTATATAT CTGACTATAA TTTTTATGAC ATATTTAATG ACCATTCGAT AAATCAAGTA 
CCTGAGTCAA CAATTCTTAC AGACTCTGAT GATAGCAATA ATTATAGTGA TCAAGAAACA 
GGGATTGATA TGAGCATGGT AAAACGTTTC AAACCAGTTG GCGAAGAGAG TAATGAGTTA 
AGCATACGCT CACAACGCCA GTTTGATGAA GATGTTGATG ATTATAATAT GCCAGATGAA 
GAACTTGCTC GTTTAGTTAA TCAAACAAAT GATGACGATT TTTCAAGCAC TAGTGAGGAA 
TTAATCACTG TAGAAGAAAA AATTATTGAG CCATGTGAAG CTGCAAAGAA TCATGTCCTG 
AAGCGTGAGT TTGGGGTTGG TGCAGATACT TCAGGTGAAT CATTATCTGC AAAGGAAGGA 
GATGCAACTG TTCCAGAGAG AATGTATAAA TTAGTTTTAG TTGGAAATGC AGCTGTTGGA 
AAATCAAGTT TTATTATTCG TTTATGCAAG AATAAATTCT ATTCTTCTCT TAACTCAACT 
CTTGGTGTTG ATTTTCAAAT AAAAAACCTC CAATTAAATG GAAAAACAGT AGCTCTGCAG 
CTCTGGGACA CGGCTGGACA AGAAAGGTTT CGAAGTATTG CAAAAAGTTA CTTTCGAAAA 
GTTGATGGCG TTATACTCAT GTATGATGTA ACATGTGAAA GTTCTTTTAT GGATGTTCGA 
GATTGGCTGG AATCTATAAA AGAGGGATCC TCAAAAACAG TTCCTATCAT AATTTGCGGC 
AATAAAACGG ACCTTCGACT TGATGCACGT CGGAAAGGTA TGCCAGTTGT CAGTCAAGAA 
CAAGGTCAAA GGTTAGCAAA TGACATTGGA GCTCTTTTTA TTGAAACCAG CGCAAAAGAA 
GGAACAAACA TCACTAACAC ATGCATAGAA TTAACCAAAC AATTAATTCA AAATGATGAA 
AAACGATTGT CTGCCACAGG AATGCAACTA AATAAACAGC CGAGTCAAAC AAAAAAAAAT 
GGTTGCTGTC GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100214119 ( XM_012705303.1 ) cDNA ORF clone, Hydra vulgaris -> XP_012560757.1 Hydra vulgaris ras and EF-hand domain-containing protein homolog (LOC100214119), transcript variant X4, mRNA.

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CloneID	OHc18051
Clone ID Related Accession (Same CDS sequence)	XM_012705303.1
Accession Version	XM_012705303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433606400000
Organism	Hydra vulgaris
Product	ras and EF-hand domain-containing protein homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004166982.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hydra vulgaris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTAGCA GAAAGGTTTA CAAGGAATTG AAAGCAAAGA GGAGATCTTT AGATTCTAAA 
GTACAGGAAT ACCTTGATGA TTTATATGAG ATTGCCAGAT CCAGAGATGA TCCGCTGCTT 
CTTGATTCCT TGGAAAATTT TCTTGCTGCA ACAGTAGATA ATTTATTGAG AAAACAGGCA 
GAAAATGAAT ACTTGGAGCT TTCTCTTAAA AGAGCAACAT TAAAGAATAA TGAGTTTAGT 
TCGCAGCTTG AACAAGAAAT GGATCAACAT GTTCAAATGC TGGAGGAGAA AATTCGACAA 
GAAGAACAGC AAAAACTGGC TATTCAAAAA CAAGAAGCCT ATCAACAGTT AATAATTGCT 
CAAAAGGAAC TGGCTGAAAT GGAGAATAAA GTTAAAATGC TAGAAAAACA AGTCGAGGAG 
AAATCAGTTA AAACTGTCAT TCCTAATACA ATTTTAGAAG AAATAAAGCT GAAGGAAAAG 
GAAAATGAAA CTCTTCATCT TCGTTTGCTT GAATTTCAAA CACAAATGGC TCTTATGAGA 
ACTGAAGTTG CAAAACTTAA GTGTGAATAT GATGAACAAT CTGATCGTCT TCAAAAAGAG 
CGTGTAACTG TTTTAGATTG TGTCCAAGAG CAAGAAAATT TAACACGACA ATTACATTTG 
CTACATGAGG CAAATAAAAA GTTGCACGAT ACTAATGATG ATCTTCGTTC AGCAATGGAG 
GTCAAGCGCT CGAACAGTTA TTTTATTGGC TCTCCAGTAA AACGTACTAC AAGTTTGGTG 
TTAACTAAGT ATAGACAGCC GAATTTGCAC AGTTCATTCC GAAGTGATCC TGGACCAGAT 
TTAAATCTTA ATGACTTTGA TGACACAGAT ATTGAGGATG CTTCAGGACG ATCAATTATA 
CCTTACGATG AAGAAGTCAT TGACAAATCT TCACTCATGG CAGAATTAAT GGAGGCTGAT 
GACCAAGTTC ATAATGGCTA TATGTCTGGA AAAAAACCTG TTAGAGTACT CAGTCTTGAA 
AACTCTGCTT CATCTACCCC TCAAAAACAT GTTGATAGAG AGCGGTTTGT ACGTCAACTA 
GAGGTTTTGT GCGAAACAAA TAGAAGATTA GGTGAAAGTA ATGATGAACT TAGAGAAGCA 
CTAGGAATTT TATCAGAGCG ACTGCGACCA CCAGATTTGA TAAAAAAATC CTCTGGTTGT 
CAATCAGCTC CAGTGACTCC TTCAAGAAGA ATTTACAGCT CTCCCATCTC CAGCTCTCCT 
CAAAAGTCGG CAGAATCATC CCACAAAGGT TTTGTTGACA CAATGAAAAA GTCTGCTGAA 
TCATCCCACA AAGTTTTTGT TGATACTGCA AAAAAAGAAG TAAAACTTCA ATGTTTTACT 
CCTTTTAATG AGTCTTCTGT GGATTCTACT ACATTTAACA CTGATGATGC TACTGAATCT 
TCAATCTTAA AAGGAGAATA TCATAGAAGA ATGGTTGATG ATCGCAGTTA TAATAATAAT 
AATAGTTTAA CAGATTCAAG TAATGAAGCT ACAAACATTG AATCTACTGC ATATGATGAT 
GAATCTACTG TTTATGATGG GGAATCAACT GTCTATGATG GAGAATCAAC TGTCTATGAT 
GGAGAATCTA CTGTTTTAAA CACCTGTTGT TGTGGTAAAA TAATTTTTGA AGACAAAAAT 
TTGTGCAAAG ATTGCACTTT TACAGACACT GAAAAAGGCA CTGGAATTTC AGATACATTT 
TTGTCAAGTG ATCAGAGTTT GTTTGATAAT ATTCACACTG AAAGTTCTGA CTTGAATAGT 
TTACATGTCC TACCAGCAAA AACTGATATG TGCACTCAAG TAGATAATAA TCTTAGTTTA 
TGTGCTATTT CTGAAAAACA TGAAGTTTCA GTTCAGTGTG AATTCCCTCG TGAACTTCCA 
AGTAAAAATG AACTAAATGT CAATCAGTCT TTTGAACATA ATAAAACAAA ATTGCAAAGA 
AAAGAAGCAA AAATGAATAA TCATTTACTA AACAAAACCC AATCAACAGA ATATCAGTTT 
AAAGAAATAG ATGAGATAGT GCATGATATA GTTGAATCAA GTCTTGATAA AGCTGATAGT 
ATAAGCTTGG ATCATATTTA TGAAAAACAC AATAGTAAGG AAGTTAGTCA ACAAATTTCT 
GAAAAACCTT ACTATATATC TGACTATAAT TTTTATGACA TATTTAATGA CCATTCGATA 
AATCAAGTAC CTGAGTCAAC AATTCTTACA GACTCTGATG ATAGCAATAA TTATAGTGAT 
CAAGAAACAG GGATTGATAT GAGCATGGTA AAACGTTTCA AACCAGTTGG CGAAGAGAGT 
AATGAGGACG CTACTTACGA AACTACTAAT GAATACATGA CTAACTACTC TTGGTCTGTA 
AGCTCTGGAT CTGCATTTTT ATTGAAAAAA AAAAAAAAAG AGTTAAGCAT ACGCTCACAA 
CGCCAGTTTG ATGAAGATGT TGATGATTAT AATATGCCAG ATGAAGAACT TGCTCGTTTA 
GTTAATCAAA CAAATGATGA CGATTTTTCA AGCACTAGTG AGGAATTAAT CACTGTAGAA 
GAAAAAATTA TTGAGCCATG TGAAGCTGCA AAGAATCATG TCCTGAAGCG TGAGTTTGGG 
GTTGGTGCAG ATACTTCAGG TGAATCATTA TCTGCAAAGG AAGGAGATGC AACTGTTCCA 
GAGAGAATGT ATAAATTAGT TTTAGTTGGA AATGCAGCTG TTGGAAAATC AAGTTTTATT 
ATTCGTTTAT GCAAGAATAA ATTCTATTCT TCTCTTAACT CAACTCTTGG TGTTGATTTT 
CAAATAAAAA ACCTCCAATT AAATGGAAAA ACAGTAGCTC TGCAGCTCTG GGACACGGCT 
GGACAAGAAA GGTTTCGAAG TATTGCAAAA AGTTACTTTC GAAAAGTTGA TGGCGTTATA 
CTCATGTATG ATGTAACATG TGAAAGTTCT TTTATGGATG TTCGAGATTG GCTGGAATCT 
ATAAAAGAGG GATCCTCAAA AACAGTTCCT ATCATAATTT GCGGCAATAA AACGGACCTT 
CGACTTGATG CACGTCGGAA AGGTATGCCA GTTGTCAGTC AAGAACAAGG TCAAAGGTTA 
GCAAATGACA TTGGAGCTCT TTTTATTGAA ACCAGCGCAA AAGAAGGAAC AAACATCACT 
AACACATGCA TAGAATTAAC CAAACAATTA ATTCAAAATG ATGAAAAACG ATTGTCTGCC 
ACAGGAATGC AACTAAATAA ACAGCCGAGT CAAACAAAAA AAAATGGTTG CTGTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012560757.1
CDS	60..3359
Translation	MCSRKVYKELKAKRRSLDSKVQEYLDDLYEIARSRDDPLLLDSLENFLAATVDNLLRKQAENEYLELSLKRATLKNNEFSSQLEQEMDQHVQMLEEKIRQEEQQKLAIQKQEAYQQLIIAQKELAEMENKVKMLEKQVEEKSVKTVIPNTILEEIKLKEKENETLHLRLLEFQTQMALMRTEVAKLKCEYDEQSDRLQKERVTVLDCVQEQENLTRQLHLLHEANKKLHDTNDDLRSAMEVKRSNSYFIGSPVKRTTSLVLTKYRQPNLHSSFRSDPGPDLNLNDFDDTDIEDASGRSIIPYDEEVIDKSSLMAELMEADDQVHNGYMSGKKPVRVLSLENSASSTPQKHVDRERFVRQLEVLCETNRRLGESNDELREALGILSERLRPPDLIKKSSGCQSAPVTPSRRIYSSPISSSPQKSAESSHKGFVDTMKKSAESSHKVFVDTAKKEVKLQCFTPFNESSVDSTTFNTDDATESSILKGEYHRRMVDDRSYNNNNSLTDSSNEATNIESTAYDDESTVYDGESTVYDGESTVYDGESTVLNTCCCGKIIFEDKNLCKDCTFTDTEKGTGISDTFLSSDQSLFDNIHTESSDLNSLHVLPAKTDMCTQVDNNLSLCAISEKHEVSVQCEFPRELPSKNELNVNQSFEHNKTKLQRKEAKMNNHLLNKTQSTEYQFKEIDEIVHDIVESSLDKADSISLDHIYEKHNSKEVSQQISEKPYYISDYNFYDIFNDHSINQVPESTILTDSDDSNNYSDQETGIDMSMVKRFKPVGEESNEDATYETTNEYMTNYSWSVSSGSAFLLKKKKKELSIRSQRQFDEDVDDYNMPDEELARLVNQTNDDDFSSTSEELITVEEKIIEPCEAAKNHVLKREFGVGADTSGESLSAKEGDATVPERMYKLVLVGNAAVGKSSFIIRLCKNKFYSSLNSTLGVDFQIKNLQLNGKTVALQLWDTAGQERFRSIAKSYFRKVDGVILMYDVTCESSFMDVRDWLESIKEGSSKTVPIIICGNKTDLRLDARRKGMPVVSQEQGQRLANDIGALFIETSAKEGTNITNTCIELTKQLIQNDEKRLSATGMQLNKQPSQTKKNGCCR

Target ORF information:

RefSeq Version	XM_012705303.1
Organism	Hydra vulgaris
Definition	Hydra vulgaris ras and EF-hand domain-containing protein homolog (LOC100214119), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012705303.1

ORF Insert Sequence:

ATGTGTAGCA GAAAGGTTTA CAAGGAATTG AAAGCAAAGA GGAGATCTTT AGATTCTAAA 
GTACAGGAAT ACCTTGATGA TTTATATGAG ATTGCCAGAT CCAGAGATGA TCCGCTGCTT 
CTTGATTCCT TGGAAAATTT TCTTGCTGCA ACAGTAGATA ATTTATTGAG AAAACAGGCA 
GAAAATGAAT ACTTGGAGCT TTCTCTTAAA AGAGCAACAT TAAAGAATAA TGAGTTTAGT 
TCGCAGCTTG AACAAGAAAT GGATCAACAT GTTCAAATGC TGGAGGAGAA AATTCGACAA 
GAAGAACAGC AAAAACTGGC TATTCAAAAA CAAGAAGCCT ATCAACAGTT AATAATTGCT 
CAAAAGGAAC TGGCTGAAAT GGAGAATAAA GTTAAAATGC TAGAAAAACA AGTCGAGGAG 
AAATCAGTTA AAACTGTCAT TCCTAATACA ATTTTAGAAG AAATAAAGCT GAAGGAAAAG 
GAAAATGAAA CTCTTCATCT TCGTTTGCTT GAATTTCAAA CACAAATGGC TCTTATGAGA 
ACTGAAGTTG CAAAACTTAA GTGTGAATAT GATGAACAAT CTGATCGTCT TCAAAAAGAG 
CGTGTAACTG TTTTAGATTG TGTCCAAGAG CAAGAAAATT TAACACGACA ATTACATTTG 
CTACATGAGG CAAATAAAAA GTTGCACGAT ACTAATGATG ATCTTCGTTC AGCAATGGAG 
GTCAAGCGCT CGAACAGTTA TTTTATTGGC TCTCCAGTAA AACGTACTAC AAGTTTGGTG 
TTAACTAAGT ATAGACAGCC GAATTTGCAC AGTTCATTCC GAAGTGATCC TGGACCAGAT 
TTAAATCTTA ATGACTTTGA TGACACAGAT ATTGAGGATG CTTCAGGACG ATCAATTATA 
CCTTACGATG AAGAAGTCAT TGACAAATCT TCACTCATGG CAGAATTAAT GGAGGCTGAT 
GACCAAGTTC ATAATGGCTA TATGTCTGGA AAAAAACCTG TTAGAGTACT CAGTCTTGAA 
AACTCTGCTT CATCTACCCC TCAAAAACAT GTTGATAGAG AGCGGTTTGT ACGTCAACTA 
GAGGTTTTGT GCGAAACAAA TAGAAGATTA GGTGAAAGTA ATGATGAACT TAGAGAAGCA 
CTAGGAATTT TATCAGAGCG ACTGCGACCA CCAGATTTGA TAAAAAAATC CTCTGGTTGT 
CAATCAGCTC CAGTGACTCC TTCAAGAAGA ATTTACAGCT CTCCCATCTC CAGCTCTCCT 
CAAAAGTCGG CAGAATCATC CCACAAAGGT TTTGTTGACA CAATGAAAAA GTCTGCTGAA 
TCATCCCACA AAGTTTTTGT TGATACTGCA AAAAAAGAAG TAAAACTTCA ATGTTTTACT 
CCTTTTAATG AGTCTTCTGT GGATTCTACT ACATTTAACA CTGATGATGC TACTGAATCT 
TCAATCTTAA AAGGAGAATA TCATAGAAGA ATGGTTGATG ATCGCAGTTA TAATAATAAT 
AATAGTTTAA CAGATTCAAG TAATGAAGCT ACAAACATTG AATCTACTGC ATATGATGAT 
GAATCTACTG TTTATGATGG GGAATCAACT GTCTATGATG GAGAATCAAC TGTCTATGAT 
GGAGAATCTA CTGTTTTAAA CACCTGTTGT TGTGGTAAAA TAATTTTTGA AGACAAAAAT 
TTGTGCAAAG ATTGCACTTT TACAGACACT GAAAAAGGCA CTGGAATTTC AGATACATTT 
TTGTCAAGTG ATCAGAGTTT GTTTGATAAT ATTCACACTG AAAGTTCTGA CTTGAATAGT 
TTACATGTCC TACCAGCAAA AACTGATATG TGCACTCAAG TAGATAATAA TCTTAGTTTA 
TGTGCTATTT CTGAAAAACA TGAAGTTTCA GTTCAGTGTG AATTCCCTCG TGAACTTCCA 
AGTAAAAATG AACTAAATGT CAATCAGTCT TTTGAACATA ATAAAACAAA ATTGCAAAGA 
AAAGAAGCAA AAATGAATAA TCATTTACTA AACAAAACCC AATCAACAGA ATATCAGTTT 
AAAGAAATAG ATGAGATAGT GCATGATATA GTTGAATCAA GTCTTGATAA AGCTGATAGT 
ATAAGCTTGG ATCATATTTA TGAAAAACAC AATAGTAAGG AAGTTAGTCA ACAAATTTCT 
GAAAAACCTT ACTATATATC TGACTATAAT TTTTATGACA TATTTAATGA CCATTCGATA 
AATCAAGTAC CTGAGTCAAC AATTCTTACA GACTCTGATG ATAGCAATAA TTATAGTGAT 
CAAGAAACAG GGATTGATAT GAGCATGGTA AAACGTTTCA AACCAGTTGG CGAAGAGAGT 
AATGAGGACG CTACTTACGA AACTACTAAT GAATACATGA CTAACTACTC TTGGTCTGTA 
AGCTCTGGAT CTGCATTTTT ATTGAAAAAA AAAAAAAAAG AGTTAAGCAT ACGCTCACAA 
CGCCAGTTTG ATGAAGATGT TGATGATTAT AATATGCCAG ATGAAGAACT TGCTCGTTTA 
GTTAATCAAA CAAATGATGA CGATTTTTCA AGCACTAGTG AGGAATTAAT CACTGTAGAA 
GAAAAAATTA TTGAGCCATG TGAAGCTGCA AAGAATCATG TCCTGAAGCG TGAGTTTGGG 
GTTGGTGCAG ATACTTCAGG TGAATCATTA TCTGCAAAGG AAGGAGATGC AACTGTTCCA 
GAGAGAATGT ATAAATTAGT TTTAGTTGGA AATGCAGCTG TTGGAAAATC AAGTTTTATT 
ATTCGTTTAT GCAAGAATAA ATTCTATTCT TCTCTTAACT CAACTCTTGG TGTTGATTTT 
CAAATAAAAA ACCTCCAATT AAATGGAAAA ACAGTAGCTC TGCAGCTCTG GGACACGGCT 
GGACAAGAAA GGTTTCGAAG TATTGCAAAA AGTTACTTTC GAAAAGTTGA TGGCGTTATA 
CTCATGTATG ATGTAACATG TGAAAGTTCT TTTATGGATG TTCGAGATTG GCTGGAATCT 
ATAAAAGAGG GATCCTCAAA AACAGTTCCT ATCATAATTT GCGGCAATAA AACGGACCTT 
CGACTTGATG CACGTCGGAA AGGTATGCCA GTTGTCAGTC AAGAACAAGG TCAAAGGTTA 
GCAAATGACA TTGGAGCTCT TTTTATTGAA ACCAGCGCAA AAGAAGGAAC AAACATCACT 
AACACATGCA TAGAATTAAC CAAACAATTA ATTCAAAATG ATGAAAAACG ATTGTCTGCC 
ACAGGAATGC AACTAAATAA ACAGCCGAGT CAAACAAAAA AAAATGGTTG CTGTCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100214119 cDNA ORF clone, Hydra vulgaris

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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