LOC100213268 cDNA ORF clone, Hydra vulgaris

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100213268
Entrez Gene ID	100213268
Full Name	golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like
Gene Type	protein-coding
Organism	Hydra vulgaris

mRNA and Protein(s)

mRNA	Protein	Name
XM_012701897.1	XP_012557351.1	golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like

Pathways from BioSystems
Gene Ontology

The following LOC100213268 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100213268 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHc17807	XM_012701897.1 Latest version!	Hydra vulgaris golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC100213268), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100213268 ( XM_012701897.1 ) cDNA ORF clone, Hydra vulgaris -> XP_012557351.1 Hydra vulgaris golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC100213268), mRNA.

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CloneID	OHc17807
Clone ID Related Accession (Same CDS sequence)	XM_012701897.1
Accession Version	XM_012701897.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1433606400000
Organism	Hydra vulgaris
Product	golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004168338.1) and transcript sequence (GAOL01025581.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Hydra vulgaris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 173 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGTTGCCAA AAAATGCTAT TTATATAATA CAAGGAGAAC TTAATCATGT TGTTGCTGCA 
CTAAGGCGAA GTAATCGATG GAGTGGAGCT CACTTTCACC AGGAAGATGA GCAGGACCCT 
TTGATTAATA GTTTTATTCA GCTTAAAGAA ACTTTACAGG AAACCAAAGA CATAAACAGT 
TTAGATACAA ATGTTTTTCT TGGACCATTT CTTGATGTTA TTCGTTCAGA AGACACTACA 
GGACCAATAA CTGGAGTAGC GCTTTCATCA GTTAACAAAT TCTTATCATA TGGATTACTT 
GATCCTTTAT CAGAGAGTGC AAGTTCTGGA ATAGATAATT TGGCAGATGC TGTCACTCAT 
GCACGTTTTG TGGGAACAGA TACCAGCAGT GATGAAGTTG TACTTATGAA GATTCTACAG 
GTACTTCGGT CTTTACTTCT AAGTCCTGTT GGTGTTCATA TGACTAATGA ATCTGTTTGC 
GAGATTATGC AGTCTTGCTT TAGAATCTGC TTTGAAATGA GACTTAGTGA ACTTTTACGT 
CGAAGTGCTG AACAAACTTT GACAGACATG GTTCAGTTAC TCTTTTTACG GTTGCCTCAA 
TTTAATGAAG ACTCAAAAAG TATTTTATCT TCGCCACATA TTCGTAAGAT GTTCAAGAGA 
ACAGTTCCAA CATCTGGTTC AAGGAAAAAT AGAGCAAAAC CAAAGATGCG TCAATCAAAA 
TCTATGACAA CAGTTAATAC CACAACAAGT CCTTCAAATA AAAATGGTTC TGAAGATGAA 
AAGGAACTTC AAAAAATAGA AACAAAACGT TCTCATTCTG ATACAGAAGT TGTTTCTGAC 
AGCAGTGAAT GTAAAACTAT GGTTGACAGC AGTGAATGTA AAACTATGGT TGACAGCAGT 
GAATGTAAAA CTATGGTTGA AGTAGAACCT TCATCTGCAG ACGAAATGAC ATTTTTAAAA 
AAACTTCATT CACAAAATTC TAGAGAAATT GATTCGACAA CAGTTGTTGA AAATACATTA 
AGTGATTCAA CAGAAGTTAT GAAAAGTATT GAAGATATAA ACCAAGCATG TGATCTTCAA 
CTAGAATCTA CATTATCGAT GGAAATTCCA GATGGTCAAG TAAAGAGCAA GTCATGTGAT 
TCTAATAAAA ATCTCCAAAA CTTTGAAGAC CATGGCGTAA TATCATTACA ATATCCAGAC 
GAGTCAGATC CAACAGTTGG TGATGATGAG GTTGCTAGCC CTGTTCAGAT TACTTCAACA 
ACATTGAAAA CTGAAGAGAC TAATAACCAA GATTATGTGA ATACCCAGGG TGTTCGTTTC 
ACATCACATG ATAAAGAAGG AATGGGTCCT CTTATGCCTT ATGGACTTCC TTGTGTGCGC 
GAGCTGTTAC GATTTCTAAC CTCACTTATC AATCCTAACG ATCGACACAA TACCAACACA 
ATGATACATA TTGGTTTGTC ATTAATCATA GTGGCATTAG AATCTGGAGC TAGCCATATT 
GGACACTTTG CTTCACTTTT GCATATTGTA AAAGATGACT TATGTAAAAA TCTATTTTCG 
CTTCTTCAAT GTGATATTCA CATTCTATTT TCATTATCAA TGCGTGTATG TTTCCTTTTG 
TTTGAAGCAC TTCGTCAAAA TTTAAAGTTT CAAAACGAGC GCTTTTTCTT GAAACTCATG 
GAAATTTTAT CAATTGAGAA TCTTAATGTT CCTTATGAGA AGAGAGAAAT GGCACTAGAA 
TATGTTAATC AGCTGTTTCA TATTGATGGA TTAGTACAAG AACTTTATGT CAATTTTGAT 
TGTGATCTGC ACTGTGCAAA CATACTAGAA GATCTTTGTA AAACTCTTTC AAAGAATGCT 
TTCCGTCCTG GCAATCTGTC ATCTGTAAAT TTTTTGTCTC TTGATGCATT GCTTACAATT 
ATCAATGAGT TTGAAGCACA TTGCAAGGAT GTAAAAGGAA ATCACCAGGA TGAAAAATCT 
TCAGCTGTTG ACTTTATAGT GGAATATGAA TCAGAAGTTG ATGATGTTGA TCATAGTATA 
GGATGCACTG CTCTCTCCAC ATCAGGTTAT GCAACTGGTC AACGATTTCT TGGTAATTCT 
AGTGTTCCAA GCAAGGATTA CAAGACTGAT AGTTTTTCTA GCAATCTACC TTCTCGAGAA 
GAAATTTCTG CTAAAAAAAT TAGGAAAAAA TTGTTGTCAC ATGGTATTGA TGAATTTAAT 
AAAAAGCCAA AAAGTGGTAT AGATTATTTG CAGGAGCATG GTTTACTTTC AGCTAATTTT 
CTTCCACAAG ATTTAATTGT TTATTTGAAA GAAAATCCTC GCGTGGACAA GAAAACACTT 
GGAGATTTTA TTGGTACTCG TAAAAATGCT GAATATCTTA AAGCATTTGT TGGATCATTT 
GCAATGAAAA ATCTGCGTAT TGATGAGGCT CTTCGAAATT TTTTGGAATC TTTTCGACTT 
CCTGGTGAAA GTCCAGTCAT ATCTTTGATA TTGGAGGAGT TTTCTTCAGT TTATTTTAAT 
AATAATCCTG ACCCATACAA GAATACAGAT GCTGCATTCA CTATGGCTTA TGCTGTCATC 
ATGTTGAATG TTGATCAACA CAACAAAAAT GTAAAGCAGC AAAAATCTAT GAACCTTGAT 
GATTTTAAAA GAAATCTTCG TAAAACAAAT GGCGGAGAAG ATTTTCCAGC AGAGTTATTA 
GAAGAAATTT TTGAAAACAT AAGAACGAAT GAAATTGTTA TGCCTTCAGA ACGAATTGGT 
AAAGTAAAGG AAGCATACGA GTGGAAGGTG TTAATGCAAA AATGTGAAGG CCCTGATTCA 
CATTATCTCC AAGTTAAATC AGCTCTTTAT GACGAGGAAT TATTTCTAGT TGTTTGGGGT 
CCTACAGTTG CTGCTTTATC ATATATATAT GATAATGGAA GTGACAAAGT ACATATTCAG 
AAAGCTCTTG CAGGATTTAA GAAGTGTGCT GTGATCTCTG CTCATTATGA ACTGTCAGAT 
GTGTTTGATA ATCTTCTTAT ATCGCTTTGT AAATTTACAA GTCTCCTTGT GACTGGTCAG 
CTTAATGACA ATCTTTCTGT TGTGTTTGGA AATAACCTTA AATCACAACT GAGCATGAGA 
ACTGTTTTTG TTCTTGCTCA TAAGCAAGGC GATATGCTTA GAGAAGGTTG GCAAAATTTA 
TTGGTGTGTC TTATGCAGCT GTTCAAAGGA AAATTATTAC CACCTTCACT TACTGAGGTT 
GAAGATTTTG TAGAACAAAG TGGAAAAATT AGCCTGATAA AAGAAGAAGT GCAACCAGTC 
GCAAAGCAGG ACAATACTGG TGTTTTTGCA GCTTTTTATT CCTACATGGT TGCAAATCCA 
GACACTCCTG CAAAATCTGT TTTGTCTGTA GAAGATCGAC AATCACAAGA AGCTGCACTG 
AATTGTATAA AGGAGTGTCA CCCTGAAAAT ATAATAACAG AAAGTAAATT TTTAAGACCG 
GAGTCTTTGC AAGAACTTGT TAAGAGTCTG ATAATTGCAT CAAAACCCTT GCACTTATAT 
GAATCTACTG ATGTCCCTTA TGATGAGGAT GCTGCTGTCT TTTTTCTTGA GTTTTTAATA 
AAAGTTGCTT TACAGAACAA AGATCGCATA TCATTGTTGT GGCATGCTCT TCGAGAACAT 
TTATCCAATA TAATCATAAG TGCCCCAAAA GTGAGTTTTT TAGTAGAAAG AGCTGTTGTT 
GGTTTACTTC GAATAGCTAT AAGACTTTTG GGTCGTGATG AAATTTCATC TCAAATATTA 
ATTACTTTAC GGATTCTACT CATGATGCAC CCTAAAGTTT TGCTCAGTTG CTCTAAACAA 
ATTTCATACG GCCTTCATGA GTTATTGCGA ACTAATGCTG TTAATTTGCG ATTTTCAAGA 
GATTGGATTA CAATATTAAC AATACTTCAG GTTGTGGGAG CAGGAGCAAT TCCTCCTATT 
GTTAAACCAG GTGTAAGTCT AACTTTAGTA TCTGGAGACA TTTACTCTGT TTTACAAGAG 
GAAAAAATTG ATACAGAAAC TGGAGAGTTT TACAGTAATA GCATTGAGCA AATAGATAGA 
GGCTATGTTG CTGATGTAAA AGAAAACACC TACAGTATGA AACATCAAAA TGAACAATGG 
CTTTTAATAA ATAAAAATGA GGATGAAGGG CTACCTATAA ATCAATATGA ACTCAAGTTT 
AAGGAAAAAT TAAACAAACA CAATTGTAAA GTTTTTATTA AGGCTTCGTC TTCTATTAGT 
TTTATAGTCA GAGATATGGC TCATGTTAAT GACTATAACA TATTTCAATG TATCTATGCA 
GTTTTATTGT TTGGTGAAGC AAGCTCAAAT GGTGGTTTAA AGTATAGATT GGATGACTCT 
GTTATTAATG AAATTGAAAA AGAGCTTAGA CACATTCCTG AAAAGCCTAA ACAAAAAGGC 
AAGCGAGGGT CATCGCCAAA AAGAGATAGC AAAAAGTCAC ATCAAGCATT TGATTCCCCT 
AAAAAAGAAG ATGAAGTTGA GTTTAATGAA AACACTGGTA ATATTTATGA TGCAATCTCT 
TTACAGCTCC TTGATCTTAT GTATGCTTTA CACACAAATG CTTCAGGTAT ACTTGGAAAC 
ATGCAGTGGA ATGATTATCA AGACAAACAA CAGGCTGAAC TAAAAGCCAG ATGGAGTAGT 
AAAGAAAGTA TTGATCAACT TCCCAGTACT TTTAAAGCTG ATAGCGATAA TGCCATGTCA 
TTGTTATGGG TAAAGTGCTG GTGTCCACTC CTTCAAGGAA TTGCTCGACT TTGTTGTGAT 
ACTCGACGTG GTATTCGAAT GACTGCTTTA ACAGTGTTGC AACGATCAAT GTTAGCAGAT 
GACCTCCAAC TACTGTCTGC TGCTGAGTGG GAGAATTGTT TTAACCAGGT TTTATTTCCT 
ATGTTGTCTA ATTTACTTGA ACTTTCAACG GATTGGGATC CAAATGGTTT GGAAGAGACT 
AGAATGAGAG CAGCTACATT GCTATGCAAA GCTTTTCTTT TGCATTTAAA CACTTTAATA 
TCATTGGCAA CATTTACAGC ATTATGGATG ACAATGCTTG ACTTTATGGA CAAGTATATG 
CATGCCGACA ATAGTGACTT ACTTTTTGAG GCTGTCCCGG AGTCACTTAA AAATATGCTG 
CTTGTGATGT CAACAGCGGG AATATTTGAA CATCATCAAG ATATACCAGA GAATGAAAAT 
CCAGATAAAA AAACTAGACG GTACTCAGCA TTGTGGCAGG TTACCTGGGA CCGAATAGAC 
TGCTTTCTGC CAAATTTAAA AGATGATCTT TTAGCGACTC GGTCACCGGT TTCATTGCGT 
GCTTCGTCTC CTCCGGAACC AGATGTTCCT AGACTAATGA CTATTCATAA CATAGAAGAT 
GGCGATTCTG TGCAAATTTT AAGAACTCAT TCACCTGAAC AAAATATCGA GTCTTCGTCG 
TCTTTAAAAG TTAGCTCCGT TTCTTTGACA AAACCACTGT TAGATATTGT TTCTACTCCT 
GTATCATCAC CAGTTTCACA ACGTGCTTCA AGCCCATTAT TAACAGACGA ACCTTCTGGA 
TTAAATGTTC AACTCTTTAC TCCTTTGCCT CTGATTAGTC CTCCTGGCAG TGTGCACGCT 
GTATCAGAAA CTACGAATGT GCATCCAGTA GCAGTCCTTT TGCCACCCAT AGCTATGTTA 
GAACTTTCAC CTAATGATAG GAAAGAGGAA ACTTCGAAAA AAATACTTTT TAATGATGGA 
AACACTGATG TTAGAGCTGT CTTCGATATA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012557351.1
CDS	29..5821
Translation	MLPKNAIYIIQGELNHVVAALRRSNRWSGAHFHQEDEQDPLINSFIQLKETLQETKDINSLDTNVFLGPFLDVIRSEDTTGPITGVALSSVNKFLSYGLLDPLSESASSGIDNLADAVTHARFVGTDTSSDEVVLMKILQVLRSLLLSPVGVHMTNESVCEIMQSCFRICFEMRLSELLRRSAEQTLTDMVQLLFLRLPQFNEDSKSILSSPHIRKMFKRTVPTSGSRKNRAKPKMRQSKSMTTVNTTTSPSNKNGSEDEKELQKIETKRSHSDTEVVSDSSECKTMVDSSECKTMVDSSECKTMVEVEPSSADEMTFLKKLHSQNSREIDSTTVVENTLSDSTEVMKSIEDINQACDLQLESTLSMEIPDGQVKSKSCDSNKNLQNFEDHGVISLQYPDESDPTVGDDEVASPVQITSTTLKTEETNNQDYVNTQGVRFTSHDKEGMGPLMPYGLPCVRELLRFLTSLINPNDRHNTNTMIHIGLSLIIVALESGASHIGHFASLLHIVKDDLCKNLFSLLQCDIHILFSLSMRVCFLLFEALRQNLKFQNERFFLKLMEILSIENLNVPYEKREMALEYVNQLFHIDGLVQELYVNFDCDLHCANILEDLCKTLSKNAFRPGNLSSVNFLSLDALLTIINEFEAHCKDVKGNHQDEKSSAVDFIVEYESEVDDVDHSIGCTALSTSGYATGQRFLGNSSVPSKDYKTDSFSSNLPSREEISAKKIRKKLLSHGIDEFNKKPKSGIDYLQEHGLLSANFLPQDLIVYLKENPRVDKKTLGDFIGTRKNAEYLKAFVGSFAMKNLRIDEALRNFLESFRLPGESPVISLILEEFSSVYFNNNPDPYKNTDAAFTMAYAVIMLNVDQHNKNVKQQKSMNLDDFKRNLRKTNGGEDFPAELLEEIFENIRTNEIVMPSERIGKVKEAYEWKVLMQKCEGPDSHYLQVKSALYDEELFLVVWGPTVAALSYIYDNGSDKVHIQKALAGFKKCAVISAHYELSDVFDNLLISLCKFTSLLVTGQLNDNLSVVFGNNLKSQLSMRTVFVLAHKQGDMLREGWQNLLVCLMQLFKGKLLPPSLTEVEDFVEQSGKISLIKEEVQPVAKQDNTGVFAAFYSYMVANPDTPAKSVLSVEDRQSQEAALNCIKECHPENIITESKFLRPESLQELVKSLIIASKPLHLYESTDVPYDEDAAVFFLEFLIKVALQNKDRISLLWHALREHLSNIIISAPKVSFLVERAVVGLLRIAIRLLGRDEISSQILITLRILLMMHPKVLLSCSKQISYGLHELLRTNAVNLRFSRDWITILTILQVVGAGAIPPIVKPGVSLTLVSGDIYSVLQEEKIDTETGEFYSNSIEQIDRGYVADVKENTYSMKHQNEQWLLINKNEDEGLPINQYELKFKEKLNKHNCKVFIKASSSISFIVRDMAHVNDYNIFQCIYAVLLFGEASSNGGLKYRLDDSVINEIEKELRHIPEKPKQKGKRGSSPKRDSKKSHQAFDSPKKEDEVEFNENTGNIYDAISLQLLDLMYALHTNASGILGNMQWNDYQDKQQAELKARWSSKESIDQLPSTFKADSDNAMSLLWVKCWCPLLQGIARLCCDTRRGIRMTALTVLQRSMLADDLQLLSAAEWENCFNQVLFPMLSNLLELSTDWDPNGLEETRMRAATLLCKAFLLHLNTLISLATFTALWMTMLDFMDKYMHADNSDLLFEAVPESLKNMLLVMSTAGIFEHHQDIPENENPDKKTRRYSALWQVTWDRIDCFLPNLKDDLLATRSPVSLRASSPPEPDVPRLMTIHNIEDGDSVQILRTHSPEQNIESSSSLKVSSVSLTKPLLDIVSTPVSSPVSQRASSPLLTDEPSGLNVQLFTPLPLISPPGSVHAVSETTNVHPVAVLLPPIAMLELSPNDRKEETSKKILFNDGNTDVRAVFDI

Target ORF information:

RefSeq Version	XM_012701897.1
Organism	Hydra vulgaris
Definition	Hydra vulgaris golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1-like (LOC100213268), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012701897.1

ORF Insert Sequence:

ATGTTGCCAA AAAATGCTAT TTATATAATA CAAGGAGAAC TTAATCATGT TGTTGCTGCA 
CTAAGGCGAA GTAATCGATG GAGTGGAGCT CACTTTCACC AGGAAGATGA GCAGGACCCT 
TTGATTAATA GTTTTATTCA GCTTAAAGAA ACTTTACAGG AAACCAAAGA CATAAACAGT 
TTAGATACAA ATGTTTTTCT TGGACCATTT CTTGATGTTA TTCGTTCAGA AGACACTACA 
GGACCAATAA CTGGAGTAGC GCTTTCATCA GTTAACAAAT TCTTATCATA TGGATTACTT 
GATCCTTTAT CAGAGAGTGC AAGTTCTGGA ATAGATAATT TGGCAGATGC TGTCACTCAT 
GCACGTTTTG TGGGAACAGA TACCAGCAGT GATGAAGTTG TACTTATGAA GATTCTACAG 
GTACTTCGGT CTTTACTTCT AAGTCCTGTT GGTGTTCATA TGACTAATGA ATCTGTTTGC 
GAGATTATGC AGTCTTGCTT TAGAATCTGC TTTGAAATGA GACTTAGTGA ACTTTTACGT 
CGAAGTGCTG AACAAACTTT GACAGACATG GTTCAGTTAC TCTTTTTACG GTTGCCTCAA 
TTTAATGAAG ACTCAAAAAG TATTTTATCT TCGCCACATA TTCGTAAGAT GTTCAAGAGA 
ACAGTTCCAA CATCTGGTTC AAGGAAAAAT AGAGCAAAAC CAAAGATGCG TCAATCAAAA 
TCTATGACAA CAGTTAATAC CACAACAAGT CCTTCAAATA AAAATGGTTC TGAAGATGAA 
AAGGAACTTC AAAAAATAGA AACAAAACGT TCTCATTCTG ATACAGAAGT TGTTTCTGAC 
AGCAGTGAAT GTAAAACTAT GGTTGACAGC AGTGAATGTA AAACTATGGT TGACAGCAGT 
GAATGTAAAA CTATGGTTGA AGTAGAACCT TCATCTGCAG ACGAAATGAC ATTTTTAAAA 
AAACTTCATT CACAAAATTC TAGAGAAATT GATTCGACAA CAGTTGTTGA AAATACATTA 
AGTGATTCAA CAGAAGTTAT GAAAAGTATT GAAGATATAA ACCAAGCATG TGATCTTCAA 
CTAGAATCTA CATTATCGAT GGAAATTCCA GATGGTCAAG TAAAGAGCAA GTCATGTGAT 
TCTAATAAAA ATCTCCAAAA CTTTGAAGAC CATGGCGTAA TATCATTACA ATATCCAGAC 
GAGTCAGATC CAACAGTTGG TGATGATGAG GTTGCTAGCC CTGTTCAGAT TACTTCAACA 
ACATTGAAAA CTGAAGAGAC TAATAACCAA GATTATGTGA ATACCCAGGG TGTTCGTTTC 
ACATCACATG ATAAAGAAGG AATGGGTCCT CTTATGCCTT ATGGACTTCC TTGTGTGCGC 
GAGCTGTTAC GATTTCTAAC CTCACTTATC AATCCTAACG ATCGACACAA TACCAACACA 
ATGATACATA TTGGTTTGTC ATTAATCATA GTGGCATTAG AATCTGGAGC TAGCCATATT 
GGACACTTTG CTTCACTTTT GCATATTGTA AAAGATGACT TATGTAAAAA TCTATTTTCG 
CTTCTTCAAT GTGATATTCA CATTCTATTT TCATTATCAA TGCGTGTATG TTTCCTTTTG 
TTTGAAGCAC TTCGTCAAAA TTTAAAGTTT CAAAACGAGC GCTTTTTCTT GAAACTCATG 
GAAATTTTAT CAATTGAGAA TCTTAATGTT CCTTATGAGA AGAGAGAAAT GGCACTAGAA 
TATGTTAATC AGCTGTTTCA TATTGATGGA TTAGTACAAG AACTTTATGT CAATTTTGAT 
TGTGATCTGC ACTGTGCAAA CATACTAGAA GATCTTTGTA AAACTCTTTC AAAGAATGCT 
TTCCGTCCTG GCAATCTGTC ATCTGTAAAT TTTTTGTCTC TTGATGCATT GCTTACAATT 
ATCAATGAGT TTGAAGCACA TTGCAAGGAT GTAAAAGGAA ATCACCAGGA TGAAAAATCT 
TCAGCTGTTG ACTTTATAGT GGAATATGAA TCAGAAGTTG ATGATGTTGA TCATAGTATA 
GGATGCACTG CTCTCTCCAC ATCAGGTTAT GCAACTGGTC AACGATTTCT TGGTAATTCT 
AGTGTTCCAA GCAAGGATTA CAAGACTGAT AGTTTTTCTA GCAATCTACC TTCTCGAGAA 
GAAATTTCTG CTAAAAAAAT TAGGAAAAAA TTGTTGTCAC ATGGTATTGA TGAATTTAAT 
AAAAAGCCAA AAAGTGGTAT AGATTATTTG CAGGAGCATG GTTTACTTTC AGCTAATTTT 
CTTCCACAAG ATTTAATTGT TTATTTGAAA GAAAATCCTC GCGTGGACAA GAAAACACTT 
GGAGATTTTA TTGGTACTCG TAAAAATGCT GAATATCTTA AAGCATTTGT TGGATCATTT 
GCAATGAAAA ATCTGCGTAT TGATGAGGCT CTTCGAAATT TTTTGGAATC TTTTCGACTT 
CCTGGTGAAA GTCCAGTCAT ATCTTTGATA TTGGAGGAGT TTTCTTCAGT TTATTTTAAT 
AATAATCCTG ACCCATACAA GAATACAGAT GCTGCATTCA CTATGGCTTA TGCTGTCATC 
ATGTTGAATG TTGATCAACA CAACAAAAAT GTAAAGCAGC AAAAATCTAT GAACCTTGAT 
GATTTTAAAA GAAATCTTCG TAAAACAAAT GGCGGAGAAG ATTTTCCAGC AGAGTTATTA 
GAAGAAATTT TTGAAAACAT AAGAACGAAT GAAATTGTTA TGCCTTCAGA ACGAATTGGT 
AAAGTAAAGG AAGCATACGA GTGGAAGGTG TTAATGCAAA AATGTGAAGG CCCTGATTCA 
CATTATCTCC AAGTTAAATC AGCTCTTTAT GACGAGGAAT TATTTCTAGT TGTTTGGGGT 
CCTACAGTTG CTGCTTTATC ATATATATAT GATAATGGAA GTGACAAAGT ACATATTCAG 
AAAGCTCTTG CAGGATTTAA GAAGTGTGCT GTGATCTCTG CTCATTATGA ACTGTCAGAT 
GTGTTTGATA ATCTTCTTAT ATCGCTTTGT AAATTTACAA GTCTCCTTGT GACTGGTCAG 
CTTAATGACA ATCTTTCTGT TGTGTTTGGA AATAACCTTA AATCACAACT GAGCATGAGA 
ACTGTTTTTG TTCTTGCTCA TAAGCAAGGC GATATGCTTA GAGAAGGTTG GCAAAATTTA 
TTGGTGTGTC TTATGCAGCT GTTCAAAGGA AAATTATTAC CACCTTCACT TACTGAGGTT 
GAAGATTTTG TAGAACAAAG TGGAAAAATT AGCCTGATAA AAGAAGAAGT GCAACCAGTC 
GCAAAGCAGG ACAATACTGG TGTTTTTGCA GCTTTTTATT CCTACATGGT TGCAAATCCA 
GACACTCCTG CAAAATCTGT TTTGTCTGTA GAAGATCGAC AATCACAAGA AGCTGCACTG 
AATTGTATAA AGGAGTGTCA CCCTGAAAAT ATAATAACAG AAAGTAAATT TTTAAGACCG 
GAGTCTTTGC AAGAACTTGT TAAGAGTCTG ATAATTGCAT CAAAACCCTT GCACTTATAT 
GAATCTACTG ATGTCCCTTA TGATGAGGAT GCTGCTGTCT TTTTTCTTGA GTTTTTAATA 
AAAGTTGCTT TACAGAACAA AGATCGCATA TCATTGTTGT GGCATGCTCT TCGAGAACAT 
TTATCCAATA TAATCATAAG TGCCCCAAAA GTGAGTTTTT TAGTAGAAAG AGCTGTTGTT 
GGTTTACTTC GAATAGCTAT AAGACTTTTG GGTCGTGATG AAATTTCATC TCAAATATTA 
ATTACTTTAC GGATTCTACT CATGATGCAC CCTAAAGTTT TGCTCAGTTG CTCTAAACAA 
ATTTCATACG GCCTTCATGA GTTATTGCGA ACTAATGCTG TTAATTTGCG ATTTTCAAGA 
GATTGGATTA CAATATTAAC AATACTTCAG GTTGTGGGAG CAGGAGCAAT TCCTCCTATT 
GTTAAACCAG GTGTAAGTCT AACTTTAGTA TCTGGAGACA TTTACTCTGT TTTACAAGAG 
GAAAAAATTG ATACAGAAAC TGGAGAGTTT TACAGTAATA GCATTGAGCA AATAGATAGA 
GGCTATGTTG CTGATGTAAA AGAAAACACC TACAGTATGA AACATCAAAA TGAACAATGG 
CTTTTAATAA ATAAAAATGA GGATGAAGGG CTACCTATAA ATCAATATGA ACTCAAGTTT 
AAGGAAAAAT TAAACAAACA CAATTGTAAA GTTTTTATTA AGGCTTCGTC TTCTATTAGT 
TTTATAGTCA GAGATATGGC TCATGTTAAT GACTATAACA TATTTCAATG TATCTATGCA 
GTTTTATTGT TTGGTGAAGC AAGCTCAAAT GGTGGTTTAA AGTATAGATT GGATGACTCT 
GTTATTAATG AAATTGAAAA AGAGCTTAGA CACATTCCTG AAAAGCCTAA ACAAAAAGGC 
AAGCGAGGGT CATCGCCAAA AAGAGATAGC AAAAAGTCAC ATCAAGCATT TGATTCCCCT 
AAAAAAGAAG ATGAAGTTGA GTTTAATGAA AACACTGGTA ATATTTATGA TGCAATCTCT 
TTACAGCTCC TTGATCTTAT GTATGCTTTA CACACAAATG CTTCAGGTAT ACTTGGAAAC 
ATGCAGTGGA ATGATTATCA AGACAAACAA CAGGCTGAAC TAAAAGCCAG ATGGAGTAGT 
AAAGAAAGTA TTGATCAACT TCCCAGTACT TTTAAAGCTG ATAGCGATAA TGCCATGTCA 
TTGTTATGGG TAAAGTGCTG GTGTCCACTC CTTCAAGGAA TTGCTCGACT TTGTTGTGAT 
ACTCGACGTG GTATTCGAAT GACTGCTTTA ACAGTGTTGC AACGATCAAT GTTAGCAGAT 
GACCTCCAAC TACTGTCTGC TGCTGAGTGG GAGAATTGTT TTAACCAGGT TTTATTTCCT 
ATGTTGTCTA ATTTACTTGA ACTTTCAACG GATTGGGATC CAAATGGTTT GGAAGAGACT 
AGAATGAGAG CAGCTACATT GCTATGCAAA GCTTTTCTTT TGCATTTAAA CACTTTAATA 
TCATTGGCAA CATTTACAGC ATTATGGATG ACAATGCTTG ACTTTATGGA CAAGTATATG 
CATGCCGACA ATAGTGACTT ACTTTTTGAG GCTGTCCCGG AGTCACTTAA AAATATGCTG 
CTTGTGATGT CAACAGCGGG AATATTTGAA CATCATCAAG ATATACCAGA GAATGAAAAT 
CCAGATAAAA AAACTAGACG GTACTCAGCA TTGTGGCAGG TTACCTGGGA CCGAATAGAC 
TGCTTTCTGC CAAATTTAAA AGATGATCTT TTAGCGACTC GGTCACCGGT TTCATTGCGT 
GCTTCGTCTC CTCCGGAACC AGATGTTCCT AGACTAATGA CTATTCATAA CATAGAAGAT 
GGCGATTCTG TGCAAATTTT AAGAACTCAT TCACCTGAAC AAAATATCGA GTCTTCGTCG 
TCTTTAAAAG TTAGCTCCGT TTCTTTGACA AAACCACTGT TAGATATTGT TTCTACTCCT 
GTATCATCAC CAGTTTCACA ACGTGCTTCA AGCCCATTAT TAACAGACGA ACCTTCTGGA 
TTAAATGTTC AACTCTTTAC TCCTTTGCCT CTGATTAGTC CTCCTGGCAG TGTGCACGCT 
GTATCAGAAA CTACGAATGT GCATCCAGTA GCAGTCCTTT TGCCACCCAT AGCTATGTTA 
GAACTTTCAC CTAATGATAG GAAAGAGGAA ACTTCGAAAA AAATACTTTT TAATGATGGA 
AACACTGATG TTAGAGCTGT CTTCGATATA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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LOC100213268 cDNA ORF clone, Hydra vulgaris

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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