ALG12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ALG12
Entrez Gene ID	79087
Full Name	ALG12, alpha-1,6-mannosyltransferase
Synonyms	CDG1G,ECM39,PP14673,hALG12
General protein information	Preferred Names ALG12, alpha-1,6-mannosyltransferase Names dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase asparagine-linked glycosylation 12 homolog (S. cerevisiae, alpha-1,6-mannosyltransferase) asparagine-linked glycosylation 12 homolog (yeast, alpha-1,6-mannosyltransferase) asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog asparagine-linked glycosylation protein 12 homolog dol-P-Man dependent alpha-1,6-mannosyltransferase dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichol alpha-1,6-mannosyltransferase dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichyl-alpha-1,6-mannosyltransferase dolichyl-P-mannose:Man-7-GlcNAc-2-PP-dolichyl-alpha-6-mannosyltransferase mannosyltransferase ALG12 homolog membrane protein SB87
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.33
Summary	This gene encodes a member of the glycosyltransferase 22 family. The encoded protein catalyzes the addition of the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation. Mutations in this gene have been associated with congenital disorder of glycosylation type Ig (CDG-Ig)characterized by abnormal N-glycosylation. [provided by RefSeq, Jul 2008].
Disorder MIM:	607144
Disorder Html:	Congenital disorder of glycosylation, type Ig, 607143 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_024105.4	NP_077010.1	dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
XM_017028937.1	XP_016884426.1	dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase isoform X2
XM_011530371.2	XP_011528673.1	dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase isoform X3
XM_017028936.1	XP_016884425.1	dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase isoform X1
NM_024105.3	NP_077010.1	dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	ALG12	NP_001077448.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000102	XP_311046.4
Macaca mulatta (Rhesus monkey)	LOC715172	XP_002798466.1
Oryza sativa (rice)	Os04g0544900	NP_001053463.1
Pan troglodytes (chimpanzee)	ALG12	XP_001140006.1
Mus musculus (house mouse)	Alg12	NP_001135829.1
Gallus gallus (chicken)	ALG12	NP_001034359.2
Danio rerio (zebrafish)	alg12	NP_001092219.1
Drosophila melanogaster (fruit fly)	CG8412	NP_649939.1
Homo sapiens (human)	ALG12	NP_077010.1
Xenopus tropicalis (tropical clawed frog)	alg12	NP_001016120.1
Bos taurus (cattle)	ALG12	NP_001096816.1
Canis lupus familiaris (dog)	ALG12	XP_005625791.1
Rattus norvegicus (Norway rat)	Alg12	NP_001101574.2
Caenorhabditis elegans (roundworm)	CELE_ZC513.5	NP_505071.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
Navarini AA, Simpson MA, Weale M, Knight J, Carlavan I, Reiniche P, Burden DA, Layton A, Bataille V, Allen M, Pleass R, Pink A, Creamer D, English J, Munn S, Walton S, , Willis C, Déret S, Voegel JJ, Spector T, Smith CH, Trembath RC, Barker JN
Nature communications54020(2014 Jun)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Large-scale cDNA transfection screening for genes related to cancer development and progression.
Wan D, Gong Y, Qin W, Zhang P, Li J, Wei L, Zhou X, Li H, Qiu X, Zhong F, He L, Yu J, Yao G, Jiang H, Qian L, Yu Y, Shu H, Chen X, Xu H, Guo M, Pan Z, Chen Y, Ge C, Yang S, Gu J
Proceedings of the National Academy of Sciences of the United States of America101(44)15724-9(2004 Nov)

Congenital disorders of glycosylation: a booming chapter of pediatrics.
Jaeken J, Carchon H
Current opinion in pediatrics16(4)434-9(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ALG12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALG12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu71767	XM_017028936.1 Latest version!	Homo sapiens ALG12 alpha-1,6-mannosyltransferase (ALG12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu71769	XM_011530371.2 Latest version!	Homo sapiens ALG12 alpha-1,6-mannosyltransferase (ALG12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu93579	XM_017028937.1 Latest version!	Homo sapiens ALG12 alpha-1,6-mannosyltransferase (ALG12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu18053	NM_024105.4 Latest version!	Homo sapiens ALG12 alpha-1,6-mannosyltransferase (ALG12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu18053	NM_024105.3	Homo sapiens ALG12, alpha-1,6-mannosyltransferase (ALG12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ALG12 ( XM_017028936.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884425.1 Homo sapiens ALG12 alpha-1,6-mannosyltransferase (ALG12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu71767
Clone ID Related Accession (Same CDS sequence)	XM_017028936.1
Accession Version	XM_017028936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA AGGGGTCATC AGGCAGGCGG CCCCTGCTGC TGGGGCTGCT GGTGGCCGTA 
GCCACTGTCC ACCTGGTCAT CTGTCCCTAC ACCAAAGTGG AGGAGAGCTT CAACCTGCAG 
GCCACACATG ACCTGCTCTA CCACTGGCAA GACCTGGAGC AGTACGACCA TCTTGAGTTC 
CCCGGAGTCG TCCCCAGGAC GTTCCTCGGG CCAGTGGTGA TCGCAGTGTT CTCCAGCCCC 
GCGGTTTACG TGCTTTCGCT GTTAGAAATG TCCAAGTTTT ACTCTCAGCT AATAGTTAGA 
GGAGTGCTTG GACTCGGCGT GATTTTTGGA CTCTGGACGT TACAAAAGGA AGTGAGACGG 
CACTTCGGGG CCATGGTGGC CACCATGTTC TGCTGGGTGA CGGCCATGCA GTTCCACCTG 
ATGTTCTACT GCACGCGGAC ACTGCCCAAT GTGCTGGCCC TGCCTGTAGT CCTGCTGGCC 
CTCGCGGCCT GGCTGCGGCA CGAGTGGGCC CGCTTCATCT GGCTGTCAGC CTTCGCCATC 
ATCGTGTTCA GGGTGGAGCT GTGCCTGTTC CTGGGCCTCC TGCTGCTGCT GGCCTTGGGC 
AACCGAAAGG TTTCTGTAGT CAGAGCCCTT CGCCACGCCG TCCCGGCAGG GATCCTCTGT 
TTAGGACTGA CGGTTGCTGT GGACTCTTAT TTTTGGCGGC AGCTCACTTG GCCGGAAGGA 
AAGGTGCTTT GGTACAACAC TGTCCTGAAC AAAAGCTCCA ACTGGGGGAC CTCCCCGCTG 
CTGTGGTACT TCTACTCAGC CCTGCCCCGC GGCCTGGGCT GCAGCCTGCT CTTCATCCCC 
CTGGGCTTGG TAGACAGAAG GACGCACGCG CCGACGGTGC TGGCACTGGG CTTCATGGCA 
CTCTACTCCC TCCTGCCACA CAAGGAGCTA CGCTTCATCA TCTATGCCTT CCCCATGCTC 
AACATCACGG CTGCCAGAGG CTGCTCCTAC CTGCTGAATA ACTATAAAAA GTCTTGGCTG 
TACAAAGCGG GGTCTCTGCT TGTGATCGGA CACCTCGTGG TGAATGCCGC CTACTCAGCC 
ACGGCCCTGT ATGTGTCCCA TTTCAACTAC CCAGGTGGCG TCGCAATGCA GAGGCTGCAC 
CAGCTGGTGC CCCCCCAGAC AGACGTCCTT CTGCACATTG ACGTGGCAGC CGCCCAGACA 
GGTGTGTCTC GGTTTCTCCA AGTCAACAGC GCCTGGAGGG TCTCACTCTG TCGCCCAGGC 
AGCAGTGCAG TGACGTCATT GCAGCTTACC ACAGCCTCAA CCTCCCAGGC TCAAGTGATC 
CTCCCACCTC AGCCTCCTGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884425.1
CDS	286..1629
Translation	MAGKGSSGRRPLLLGLLVAVATVHLVICPYTKVEESFNLQATHDLLYHWQDLEQYDHLEFPGVVPRTFLGPVVIAVFSSPAVYVLSLLEMSKFYSQLIVRGVLGLGVIFGLWTLQKEVRRHFGAMVATMFCWVTAMQFHLMFYCTRTLPNVLALPVVLLALAAWLRHEWARFIWLSAFAIIVFRVELCLFLGLLLLLALGNRKVSVVRALRHAVPAGILCLGLTVAVDSYFWRQLTWPEGKVLWYNTVLNKSSNWGTSPLLWYFYSALPRGLGCSLLFIPLGLVDRRTHAPTVLALGFMALYSLLPHKELRFIIYAFPMLNITAARGCSYLLNNYKKSWLYKAGSLLVIGHLVVNAAYSATALYVSHFNYPGGVAMQRLHQLVPPQTDVLLHIDVAAAQTGVSRFLQVNSAWRVSLCRPGSSAVTSLQLTTASTSQAQVILPPQPPE

Target ORF information:

RefSeq Version	XM_017028936.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ALG12 alpha-1,6-mannosyltransferase (ALG12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028936.1

ORF Insert Sequence:

ATGGCTGGAA AGGGGTCATC AGGCAGGCGG CCCCTGCTGC TGGGGCTGCT GGTGGCCGTA 
GCCACTGTCC ACCTGGTCAT CTGTCCCTAC ACCAAAGTGG AGGAGAGCTT CAACCTGCAG 
GCCACACATG ACCTGCTCTA CCACTGGCAA GACCTGGAGC AGTACGACCA TCTTGAGTTC 
CCCGGAGTCG TCCCCAGGAC GTTCCTCGGG CCAGTGGTGA TCGCAGTGTT CTCCAGCCCC 
GCGGTTTACG TGCTTTCGCT GTTAGAAATG TCCAAGTTTT ACTCTCAGCT AATAGTTAGA 
GGAGTGCTTG GACTCGGCGT GATTTTTGGA CTCTGGACGT TACAAAAGGA AGTGAGACGG 
CACTTCGGGG CCATGGTGGC CACCATGTTC TGCTGGGTGA CGGCCATGCA GTTCCACCTG 
ATGTTCTACT GCACGCGGAC ACTGCCCAAT GTGCTGGCCC TGCCTGTAGT CCTGCTGGCC 
CTCGCGGCCT GGCTGCGGCA CGAGTGGGCC CGCTTCATCT GGCTGTCAGC CTTCGCCATC 
ATCGTGTTCA GGGTGGAGCT GTGCCTGTTC CTGGGCCTCC TGCTGCTGCT GGCCTTGGGC 
AACCGAAAGG TTTCTGTAGT CAGAGCCCTT CGCCACGCCG TCCCGGCAGG GATCCTCTGT 
TTAGGACTGA CGGTTGCTGT GGACTCTTAT TTTTGGCGGC AGCTCACTTG GCCGGAAGGA 
AAGGTGCTTT GGTACAACAC TGTCCTGAAC AAAAGCTCCA ACTGGGGGAC CTCCCCGCTG 
CTGTGGTACT TCTACTCAGC CCTGCCCCGC GGCCTGGGCT GCAGCCTGCT CTTCATCCCC 
CTGGGCTTGG TAGACAGAAG GACGCACGCG CCGACGGTGC TGGCACTGGG CTTCATGGCA 
CTCTACTCCC TCCTGCCACA CAAGGAGCTA CGCTTCATCA TCTATGCCTT CCCCATGCTC 
AACATCACGG CTGCCAGAGG CTGCTCCTAC CTGCTGAATA ACTATAAAAA GTCTTGGCTG 
TACAAAGCGG GGTCTCTGCT TGTGATCGGA CACCTCGTGG TGAATGCCGC CTACTCAGCC 
ACGGCCCTGT ATGTGTCCCA TTTCAACTAC CCAGGTGGCG TCGCAATGCA GAGGCTGCAC 
CAGCTGGTGC CCCCCCAGAC AGACGTCCTT CTGCACATTG ACGTGGCAGC CGCCCAGACA 
GGTGTGTCTC GGTTTCTCCA AGTCAACAGC GCCTGGAGGG TCTCACTCTG TCGCCCAGGC 
AGCAGTGCAG TGACGTCATT GCAGCTTACC ACAGCCTCAA CCTCCCAGGC TCAAGTGATC 
CTCCCACCTC AGCCTCCTGA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALG12 ( XM_011530371.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528673.1 Homo sapiens ALG12 alpha-1,6-mannosyltransferase (ALG12), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu71769
Clone ID Related Accession (Same CDS sequence)	XM_011530371.2
Accession Version	XM_011530371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530371.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA AGGGGTCATC AGGCAGGCGG CCCCTGCTGC TGGGGCTGCT GGTGGCCGTA 
GCCACTGTCC ACCTGGTCAT CTGTCCCTAC ACCAAAGTGG AGGAGAGCTT CAACCTGCAG 
GCCACACATG ACCTGCTCTA CCACTGGCAA GACCTGGAGC AGTACGACCA TCTTGAGTTC 
CCCGGAGTCG TCCCCAGGAC GTTCCTCGGG CCAGTGGTGA TCGCAGTGTT CTCCAGCCCC 
GCGGTTTACG TGCTTTCGCT GTTAGAAATG TCCAAGTTTT ACTCTCAGCT AATAGTTAGA 
GGAGTGCTTG GACTCGGCGT GATTTTTGGA CTCTGGACGT TACAAAAGGA AGTGAGACGG 
CACTTCGGGG CCATGGTGGC CACCATGTTC TGCTGGGTGA CGGCCATGCA GTTCCACCTG 
ATGTTCTACT GCACGCGGAC ACTGCCCAAT GTGCTGGCCC TGCCTGTAGT CCTGCTGGCC 
CTCGCGGCCT GGCTGCGGCA CGAGTGGGCC CGCTTCATCT GGCTGTCAGC CTTCGCCATC 
ATCGTGTTCA GGGTGGAGCT GTGCCTGTTC CTGGGCCTCC TGCTGCTGCT GGCCTTGGGC 
AACCGAAAGG TTTCTGTAGT CAGAGCCCTT CGCCACGCCG TCCCGGCAGG GATCCTCTGT 
TTAGGACTGA CGGTTGCTGT GGACTCTTAT TTTTGGCGGC AGCTCACTTG GCCGGAAGGA 
AAGGTGCTTT GGTACAACAC TGTCCTGAAC AAAAGCTCCA ACTGGGGGAC CTCCCCGCTG 
CTGTGGTACT TCTACTCAGC CCTGCCCCGC GGCCTGGGCT GCAGCCTGCT CTTCATCCCC 
CTGGGCTTGG TAGACAGAAG GACGCACGCG CCGACGGTGC TGGCACTGGG CTTCATGGCA 
CTCTACTCCC TCCTGCCACA CAAGGAGCTA CGCTTCATCA TCTATGCCTT CCCCATGCTC 
AACATCACGG CTGCCAGAGG CTGCTCCTAC CTGTGGCGTC GCAATGCAGA GGCTGCACCA 
GCTGGTGCCC CCCCAGACAG ACGTCCTTCT GCACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528673.1
CDS	286..1344
Translation	MAGKGSSGRRPLLLGLLVAVATVHLVICPYTKVEESFNLQATHDLLYHWQDLEQYDHLEFPGVVPRTFLGPVVIAVFSSPAVYVLSLLEMSKFYSQLIVRGVLGLGVIFGLWTLQKEVRRHFGAMVATMFCWVTAMQFHLMFYCTRTLPNVLALPVVLLALAAWLRHEWARFIWLSAFAIIVFRVELCLFLGLLLLLALGNRKVSVVRALRHAVPAGILCLGLTVAVDSYFWRQLTWPEGKVLWYNTVLNKSSNWGTSPLLWYFYSALPRGLGCSLLFIPLGLVDRRTHAPTVLALGFMALYSLLPHKELRFIIYAFPMLNITAARGCSYLWRRNAEAAPAGAPPDRRPSAH

Target ORF information:

RefSeq Version	XM_011530371.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ALG12 alpha-1,6-mannosyltransferase (ALG12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011530371.2

ORF Insert Sequence:

ATGGCTGGAA AGGGGTCATC AGGCAGGCGG CCCCTGCTGC TGGGGCTGCT GGTGGCCGTA 
GCCACTGTCC ACCTGGTCAT CTGTCCCTAC ACCAAAGTGG AGGAGAGCTT CAACCTGCAG 
GCCACACATG ACCTGCTCTA CCACTGGCAA GACCTGGAGC AGTACGACCA TCTTGAGTTC 
CCCGGAGTCG TCCCCAGGAC GTTCCTCGGG CCAGTGGTGA TCGCAGTGTT CTCCAGCCCC 
GCGGTTTACG TGCTTTCGCT GTTAGAAATG TCCAAGTTTT ACTCTCAGCT AATAGTTAGA 
GGAGTGCTTG GACTCGGCGT GATTTTTGGA CTCTGGACGT TACAAAAGGA AGTGAGACGG 
CACTTCGGGG CCATGGTGGC CACCATGTTC TGCTGGGTGA CGGCCATGCA GTTCCACCTG 
ATGTTCTACT GCACGCGGAC ACTGCCCAAT GTGCTGGCCC TGCCTGTAGT CCTGCTGGCC 
CTCGCGGCCT GGCTGCGGCA CGAGTGGGCC CGCTTCATCT GGCTGTCAGC CTTCGCCATC 
ATCGTGTTCA GGGTGGAGCT GTGCCTGTTC CTGGGCCTCC TGCTGCTGCT GGCCTTGGGC 
AACCGAAAGG TTTCTGTAGT CAGAGCCCTT CGCCACGCCG TCCCGGCAGG GATCCTCTGT 
TTAGGACTGA CGGTTGCTGT GGACTCTTAT TTTTGGCGGC AGCTCACTTG GCCGGAAGGA 
AAGGTGCTTT GGTACAACAC TGTCCTGAAC AAAAGCTCCA ACTGGGGGAC CTCCCCGCTG 
CTGTGGTACT TCTACTCAGC CCTGCCCCGC GGCCTGGGCT GCAGCCTGCT CTTCATCCCC 
CTGGGCTTGG TAGACAGAAG GACGCACGCG CCGACGGTGC TGGCACTGGG CTTCATGGCA 
CTCTACTCCC TCCTGCCACA CAAGGAGCTA CGCTTCATCA TCTATGCCTT CCCCATGCTC 
AACATCACGG CTGCCAGAGG CTGCTCCTAC CTGTGGCGTC GCAATGCAGA GGCTGCACCA 
GCTGGTGCCC CCCCAGACAG ACGTCCTTCT GCACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALG12 ( XM_017028937.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884426.1 Homo sapiens ALG12 alpha-1,6-mannosyltransferase (ALG12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu93579
Clone ID Related Accession (Same CDS sequence)	XM_017028937.1
Accession Version	XM_017028937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGAA AGGGGTCATC AGGCAGGCGG CCCCTGCTGC TGGGGCTGCT GGTGGCCGTA 
GCCACTGTCC ACCTGGTCAT CTGTCCCTAC ACCAAAGTGG AGGAGAGCTT CAACCTGCAG 
GCCACACATG ACCTGCTCTA CCACTGGCAA GACCTGGAGC AGTACGACCA TCTTGAGTTC 
CCCGGAGTCG TCCCCAGGAC GTTCCTCGGG CCAGTGGTGA TCGCAGTGTT CTCCAGCCCC 
GCGGTTTACG TGCTTTCGCT GTTAGAAATG TCCAAGTTTT ACTCTCAGCT AATAGTTAGA 
GGAGTGCTTG GACTCGGCGT GATTTTTGGA CTCTGGACGT TACAAAAGGA AGTGAGACGG 
CACTTCGGGG CCATGGTGGC CACCATGTTC TGCTGGGTGA CGGCCATGCA GTTCCACCTG 
ATGTTCTACT GCACGCGGAC ACTGCCCAAT GTGCTGGCCC TGCCTGTAGT CCTGCTGGCC 
CTCGCGGCCT GGCTGCGGCA CGAGTGGGCC CGCTTCATCT GGCTGTCAGC CTTCGCCATC 
ATCGTGTTCA GGGTGGAGCT GTGCCTGTTC CTGGGCCTCC TGCTGCTGCT GGCCTTGGGC 
AACCGAAAGG TTTCTGTAGT CAGAGCCCTT CGCCACGCCG TCCCGGCAGG GATCCTCTGT 
TTAGGACTGA CGGTTGCTGT GGACTCTTAT TTTTGGCGGC AGCTCACTTG GCCGGAAGGA 
AAGGTGCTTT GGTACAACAC TGTCCTGAAC AAAAGCTCCA ACTGGGGGAC CTCCCCGCTG 
CTGTGGTACT TCTACTCAGC CCTGCCCCGC GGCCTGGGCT GCAGCCTGCT CTTCATCCCC 
CTGGGCTTGG TAGACAGAAG GACGCACGCG CCGACGGTGC TGGCACTGGG CTTCATGGCA 
CTCTACTCCC TCCTGCCACA CAAGGAGCTA CGCTTCATCA TCTATGCCTT CCCCATGCTC 
AACATCACGG CTGCCAGAGG CTGCTCCTAC CTGCTGAATA ACTATAAAAA GTCTTGGCTG 
TACAAAGCGG GGTCTCTGCT TGTGATCGGA CACCTCGTGG TGAATGCCGC CTACTCAGCC 
ACGGCCCTGT ATGTGTCCCA TTTCAACTAC CCAGGTGGCG TCGCAATGCA GAGGCTGCAC 
CAGCTGGTGC CCCCCCAGAC AGACGTCCTT CTGCACATTG ACGTGGCAGC CGCCCAGACA 
GGTGTGTCTC GGTTTCTCCA AGTCAACAGC GCCTGGAGTT TCCCCAAATC TCTCAATAAA 
AGGAAAACTA GAACAGTAAA ACCACAATCC TCTGATGTTA TCAACAACAA CAAAAAAGAA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884426.1
CDS	286..1611
Translation	MAGKGSSGRRPLLLGLLVAVATVHLVICPYTKVEESFNLQATHDLLYHWQDLEQYDHLEFPGVVPRTFLGPVVIAVFSSPAVYVLSLLEMSKFYSQLIVRGVLGLGVIFGLWTLQKEVRRHFGAMVATMFCWVTAMQFHLMFYCTRTLPNVLALPVVLLALAAWLRHEWARFIWLSAFAIIVFRVELCLFLGLLLLLALGNRKVSVVRALRHAVPAGILCLGLTVAVDSYFWRQLTWPEGKVLWYNTVLNKSSNWGTSPLLWYFYSALPRGLGCSLLFIPLGLVDRRTHAPTVLALGFMALYSLLPHKELRFIIYAFPMLNITAARGCSYLLNNYKKSWLYKAGSLLVIGHLVVNAAYSATALYVSHFNYPGGVAMQRLHQLVPPQTDVLLHIDVAAAQTGVSRFLQVNSAWSFPKSLNKRKTRTVKPQSSDVINNNKKED

Target ORF information:

RefSeq Version	XM_017028937.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ALG12 alpha-1,6-mannosyltransferase (ALG12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028937.1

ORF Insert Sequence:

ATGGCTGGAA AGGGGTCATC AGGCAGGCGG CCCCTGCTGC TGGGGCTGCT GGTGGCCGTA 
GCCACTGTCC ACCTGGTCAT CTGTCCCTAC ACCAAAGTGG AGGAGAGCTT CAACCTGCAG 
GCCACACATG ACCTGCTCTA CCACTGGCAA GACCTGGAGC AGTACGACCA TCTTGAGTTC 
CCCGGAGTCG TCCCCAGGAC GTTCCTCGGG CCAGTGGTGA TCGCAGTGTT CTCCAGCCCC 
GCGGTTTACG TGCTTTCGCT GTTAGAAATG TCCAAGTTTT ACTCTCAGCT AATAGTTAGA 
GGAGTGCTTG GACTCGGCGT GATTTTTGGA CTCTGGACGT TACAAAAGGA AGTGAGACGG 
CACTTCGGGG CCATGGTGGC CACCATGTTC TGCTGGGTGA CGGCCATGCA GTTCCACCTG 
ATGTTCTACT GCACGCGGAC ACTGCCCAAT GTGCTGGCCC TGCCTGTAGT CCTGCTGGCC 
CTCGCGGCCT GGCTGCGGCA CGAGTGGGCC CGCTTCATCT GGCTGTCAGC CTTCGCCATC 
ATCGTGTTCA GGGTGGAGCT GTGCCTGTTC CTGGGCCTCC TGCTGCTGCT GGCCTTGGGC 
AACCGAAAGG TTTCTGTAGT CAGAGCCCTT CGCCACGCCG TCCCGGCAGG GATCCTCTGT 
TTAGGACTGA CGGTTGCTGT GGACTCTTAT TTTTGGCGGC AGCTCACTTG GCCGGAAGGA 
AAGGTGCTTT GGTACAACAC TGTCCTGAAC AAAAGCTCCA ACTGGGGGAC CTCCCCGCTG 
CTGTGGTACT TCTACTCAGC CCTGCCCCGC GGCCTGGGCT GCAGCCTGCT CTTCATCCCC 
CTGGGCTTGG TAGACAGAAG GACGCACGCG CCGACGGTGC TGGCACTGGG CTTCATGGCA 
CTCTACTCCC TCCTGCCACA CAAGGAGCTA CGCTTCATCA TCTATGCCTT CCCCATGCTC 
AACATCACGG CTGCCAGAGG CTGCTCCTAC CTGCTGAATA ACTATAAAAA GTCTTGGCTG 
TACAAAGCGG GGTCTCTGCT TGTGATCGGA CACCTCGTGG TGAATGCCGC CTACTCAGCC 
ACGGCCCTGT ATGTGTCCCA TTTCAACTAC CCAGGTGGCG TCGCAATGCA GAGGCTGCAC 
CAGCTGGTGC CCCCCCAGAC AGACGTCCTT CTGCACATTG ACGTGGCAGC CGCCCAGACA 
GGTGTGTCTC GGTTTCTCCA AGTCAACAGC GCCTGGAGTT TCCCCAAATC TCTCAATAAA 
AGGAAAACTA GAACAGTAAA ACCACAATCC TCTGATGTTA TCAACAACAA CAAAAAAGAA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALG12 ( NM_024105.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_077010.1 Homo sapiens ALG12 alpha-1,6-mannosyltransferase (ALG12), mRNA.

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CloneID	OHu18053
Clone ID Related Accession (Same CDS sequence)	NM_024105.4 , NM_024105.3
Accession Version	NM_024105.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1571932800000
Organism	Homo sapiens(human)
Product	dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB858085.1, AF318343.1, BM145575.1 and AL671710.13. On Nov 23, 2018 this sequence version replaced NM_024105.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.115912.1, SRR1803613.2325.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000330817.11/ ENSP00000333813.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGGAA AGGGGTCATC AGGCAGGCGG CCCCTGCTGC TGGGGCTGCT GGTGGCCGTA 
GCCACTGTCC ACCTGGTCAT CTGTCCCTAC ACCAAAGTGG AGGAGAGCTT CAACCTGCAG 
GCCACACATG ACCTGCTCTA CCACTGGCAA GACCTGGAGC AGTACGACCA TCTTGAGTTC 
CCCGGAGTCG TCCCCAGGAC GTTCCTCGGG CCAGTGGTGA TCGCAGTGTT CTCCAGCCCC 
GCGGTTTACG TGCTTTCGCT GTTAGAAATG TCCAAGTTTT ACTCTCAGCT AATAGTTAGA 
GGAGTGCTTG GACTCGGCGT GATTTTTGGA CTCTGGACGT TACAAAAGGA AGTGAGACGG 
CACTTCGGGG CCATGGTGGC CACCATGTTC TGCTGGGTGA CGGCCATGCA GTTCCACCTG 
ATGTTCTACT GCACGCGGAC ACTGCCCAAT GTGCTGGCCC TGCCTGTAGT CCTGCTGGCC 
CTCGCGGCCT GGCTGCGGCA CGAGTGGGCC CGCTTCATCT GGCTGTCAGC CTTCGCCATC 
ATCGTGTTCA GGGTGGAGCT GTGCCTGTTC CTGGGCCTCC TGCTGCTGCT GGCCTTGGGC 
AACCGAAAGG TTTCTGTAGT CAGAGCCCTT CGCCACGCCG TCCCGGCAGG GATCCTCTGT 
TTAGGACTGA CGGTTGCTGT GGACTCTTAT TTTTGGCGGC AGCTCACTTG GCCGGAAGGA 
AAGGTGCTTT GGTACAACAC TGTCCTGAAC AAAAGCTCCA ACTGGGGGAC CTCCCCGCTG 
CTGTGGTACT TCTACTCAGC CCTGCCCCGC GGCCTGGGCT GCAGCCTGCT CTTCATCCCC 
CTGGGCTTGG TAGACAGAAG GACGCACGCG CCGACGGTGC TGGCACTGGG CTTCATGGCA 
CTCTACTCCC TCCTGCCACA CAAGGAGCTA CGCTTCATCA TCTATGCCTT CCCCATGCTC 
AACATCACGG CTGCCAGAGG CTGCTCCTAC CTGCTGAATA ACTATAAAAA GTCTTGGCTG 
TACAAAGCGG GGTCTCTGCT TGTGATCGGA CACCTCGTGG TGAATGCCGC CTACTCAGCC 
ACGGCCCTGT ATGTGTCCCA TTTCAACTAC CCAGGTGGCG TCGCAATGCA GAGGCTGCAC 
CAGCTGGTGC CCCCCCAGAC AGACGTCCTT CTGCACATTG ACGTGGCAGC CGCCCAGACA 
GGTGTGTCTC GGTTTCTCCA AGTCAACAGC GCCTGGAGGT ACGACAAGAG GGAGGATGTG 
CAGCCGGGGA CAGGCATGCT GGCATACACA CACATCCTCA TGGAGGCGGC CCCTGGGCTC 
CTGGCCCTCT ACAGGGACAC ACACCGGGTC CTGGCCAGCG TCGTGGGGAC CACAGGTGTG 
AGTCTGAACC TGACCCAACT GCCCCCCTTC AACGTCCACC TGCAGACAAA GCTGGTGCTT 
CTGGAGAGGC TCCCCCGGCC GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077010.1
CDS	255..1721
Translation	MAGKGSSGRRPLLLGLLVAVATVHLVICPYTKVEESFNLQATHDLLYHWQDLEQYDHLEFPGVVPRTFLGPVVIAVFSSPAVYVLSLLEMSKFYSQLIVRGVLGLGVIFGLWTLQKEVRRHFGAMVATMFCWVTAMQFHLMFYCTRTLPNVLALPVVLLALAAWLRHEWARFIWLSAFAIIVFRVELCLFLGLLLLLALGNRKVSVVRALRHAVPAGILCLGLTVAVDSYFWRQLTWPEGKVLWYNTVLNKSSNWGTSPLLWYFYSALPRGLGCSLLFIPLGLVDRRTHAPTVLALGFMALYSLLPHKELRFIIYAFPMLNITAARGCSYLLNNYKKSWLYKAGSLLVIGHLVVNAAYSATALYVSHFNYPGGVAMQRLHQLVPPQTDVLLHIDVAAAQTGVSRFLQVNSAWRYDKREDVQPGTGMLAYTHILMEAAPGLLALYRDTHRVLASVVGTTGVSLNLTQLPPFNVHLQTKLVLLERLPRPS

Target ORF information:

RefSeq Version	NM_024105.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ALG12 alpha-1,6-mannosyltransferase (ALG12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024105.4

ORF Insert Sequence:

ATGGCTGGAA AGGGGTCATC AGGCAGGCGG CCCCTGCTGC TGGGGCTGCT GGTGGCCGTA 
GCCACTGTCC ACCTGGTCAT CTGTCCCTAC ACCAAAGTGG AGGAGAGCTT CAACCTGCAG 
GCCACACATG ACCTGCTCTA CCACTGGCAA GACCTGGAGC AGTACGACCA TCTTGAGTTC 
CCCGGAGTCG TCCCCAGGAC GTTCCTCGGG CCAGTGGTGA TCGCAGTGTT CTCCAGCCCC 
GCGGTTTACG TGCTTTCGCT GTTAGAAATG TCCAAGTTTT ACTCTCAGCT AATAGTTAGA 
GGAGTGCTTG GACTCGGCGT GATTTTTGGA CTCTGGACGT TACAAAAGGA AGTGAGACGG 
CACTTCGGGG CCATGGTGGC CACCATGTTC TGCTGGGTGA CGGCCATGCA GTTCCACCTG 
ATGTTCTACT GCACGCGGAC ACTGCCCAAT GTGCTGGCCC TGCCTGTAGT CCTGCTGGCC 
CTCGCGGCCT GGCTGCGGCA CGAGTGGGCC CGCTTCATCT GGCTGTCAGC CTTCGCCATC 
ATCGTGTTCA GGGTGGAGCT GTGCCTGTTC CTGGGCCTCC TGCTGCTGCT GGCCTTGGGC 
AACCGAAAGG TTTCTGTAGT CAGAGCCCTT CGCCACGCCG TCCCGGCAGG GATCCTCTGT 
TTAGGACTGA CGGTTGCTGT GGACTCTTAT TTTTGGCGGC AGCTCACTTG GCCGGAAGGA 
AAGGTGCTTT GGTACAACAC TGTCCTGAAC AAAAGCTCCA ACTGGGGGAC CTCCCCGCTG 
CTGTGGTACT TCTACTCAGC CCTGCCCCGC GGCCTGGGCT GCAGCCTGCT CTTCATCCCC 
CTGGGCTTGG TAGACAGAAG GACGCACGCG CCGACGGTGC TGGCACTGGG CTTCATGGCA 
CTCTACTCCC TCCTGCCACA CAAGGAGCTA CGCTTCATCA TCTATGCCTT CCCCATGCTC 
AACATCACGG CTGCCAGAGG CTGCTCCTAC CTGCTGAATA ACTATAAAAA GTCTTGGCTG 
TACAAAGCGG GGTCTCTGCT TGTGATCGGA CACCTCGTGG TGAATGCCGC CTACTCAGCC 
ACGGCCCTGT ATGTGTCCCA TTTCAACTAC CCAGGTGGCG TCGCAATGCA GAGGCTGCAC 
CAGCTGGTGC CCCCCCAGAC AGACGTCCTT CTGCACATTG ACGTGGCAGC CGCCCAGACA 
GGTGTGTCTC GGTTTCTCCA AGTCAACAGC GCCTGGAGGT ACGACAAGAG GGAGGATGTG 
CAGCCGGGGA CAGGCATGCT GGCATACACA CACATCCTCA TGGAGGCGGC CCCTGGGCTC 
CTGGCCCTCT ACAGGGACAC ACACCGGGTC CTGGCCAGCG TCGTGGGGAC CACAGGTGTG 
AGTCTGAACC TGACCCAACT GCCCCCCTTC AACGTCCACC TGCAGACAAA GCTGGTGCTT 
CTGGAGAGGC TCCCCCGGCC GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ALG12 ( NM_024105.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_077010.1 Homo sapiens ALG12, alpha-1,6-mannosyltransferase (ALG12), mRNA.

Price & Availability↑

CloneID	OHu18053
Clone ID Related Accession (Same CDS sequence)	NM_024105.4 , NM_024105.3
Accession Version	NM_024105.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1506700800000
Organism	Homo sapiens(human)
Product	dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB858085.1, AF318343.1 and BM145575.1. This sequence is a reference standard in the RefSeqGene project. On Jan 6, 2005 this sequence version replaced NM_024105.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.161923.1, SRR1803613.273643.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGAA AGGGGTCATC AGGCAGGCGG CCCCTGCTGC TGGGGCTGCT GGTGGCCGTA 
GCCACTGTCC ACCTGGTCAT CTGTCCCTAC ACCAAAGTGG AGGAGAGCTT CAACCTGCAG 
GCCACACATG ACCTGCTCTA CCACTGGCAA GACCTGGAGC AGTACGACCA TCTTGAGTTC 
CCCGGAGTCG TCCCCAGGAC GTTCCTCGGG CCAGTGGTGA TCGCAGTGTT CTCCAGCCCC 
GCGGTTTACG TGCTTTCGCT GTTAGAAATG TCCAAGTTTT ACTCTCAGCT AATAGTTAGA 
GGAGTGCTTG GACTCGGCGT GATTTTTGGA CTCTGGACGT TACAAAAGGA AGTGAGACGG 
CACTTCGGGG CCATGGTGGC CACCATGTTC TGCTGGGTGA CGGCCATGCA GTTCCACCTG 
ATGTTCTACT GCACGCGGAC ACTGCCCAAT GTGCTGGCCC TGCCTGTAGT CCTGCTGGCC 
CTCGCGGCCT GGCTGCGGCA CGAGTGGGCC CGCTTCATCT GGCTGTCAGC CTTCGCCATC 
ATCGTGTTCA GGGTGGAGCT GTGCCTGTTC CTGGGCCTCC TGCTGCTGCT GGCCTTGGGC 
AACCGAAAGG TTTCTGTAGT CAGAGCCCTT CGCCACGCCG TCCCGGCAGG GATCCTCTGT 
TTAGGACTGA CGGTTGCTGT GGACTCTTAT TTTTGGCGGC AGCTCACTTG GCCGGAAGGA 
AAGGTGCTTT GGTACAACAC TGTCCTGAAC AAAAGCTCCA ACTGGGGGAC CTCCCCGCTG 
CTGTGGTACT TCTACTCAGC CCTGCCCCGC GGCCTGGGCT GCAGCCTGCT CTTCATCCCC 
CTGGGCTTGG TAGACAGAAG GACGCACGCG CCGACGGTGC TGGCACTGGG CTTCATGGCA 
CTCTACTCCC TCCTGCCACA CAAGGAGCTA CGCTTCATCA TCTATGCCTT CCCCATGCTC 
AACATCACGG CTGCCAGAGG CTGCTCCTAC CTGCTGAATA ACTATAAAAA GTCTTGGCTG 
TACAAAGCGG GGTCTCTGCT TGTGATCGGA CACCTCGTGG TGAATGCCGC CTACTCAGCC 
ACGGCCCTGT ATGTGTCCCA TTTCAACTAC CCAGGTGGCG TCGCAATGCA GAGGCTGCAC 
CAGCTGGTGC CCCCCCAGAC AGACGTCCTT CTGCACATTG ACGTGGCAGC CGCCCAGACA 
GGTGTGTCTC GGTTTCTCCA AGTCAACAGC GCCTGGAGGT ACGACAAGAG GGAGGATGTG 
CAGCCGGGGA CAGGCATGCT GGCATACACA CACATCCTCA TGGAGGCGGC CCCTGGGCTC 
CTGGCCCTCT ACAGGGACAC ACACCGGGTC CTGGCCAGCG TCGTGGGGAC CACAGGTGTG 
AGTCTGAACC TGACCCAACT GCCCCCCTTC AACGTCCACC TGCAGACAAA GCTGGTGCTT 
CTGGAGAGGC TCCCCCGGCC GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077010.1
CDS	275..1741
Translation	MAGKGSSGRRPLLLGLLVAVATVHLVICPYTKVEESFNLQATHDLLYHWQDLEQYDHLEFPGVVPRTFLGPVVIAVFSSPAVYVLSLLEMSKFYSQLIVRGVLGLGVIFGLWTLQKEVRRHFGAMVATMFCWVTAMQFHLMFYCTRTLPNVLALPVVLLALAAWLRHEWARFIWLSAFAIIVFRVELCLFLGLLLLLALGNRKVSVVRALRHAVPAGILCLGLTVAVDSYFWRQLTWPEGKVLWYNTVLNKSSNWGTSPLLWYFYSALPRGLGCSLLFIPLGLVDRRTHAPTVLALGFMALYSLLPHKELRFIIYAFPMLNITAARGCSYLLNNYKKSWLYKAGSLLVIGHLVVNAAYSATALYVSHFNYPGGVAMQRLHQLVPPQTDVLLHIDVAAAQTGVSRFLQVNSAWRYDKREDVQPGTGMLAYTHILMEAAPGLLALYRDTHRVLASVVGTTGVSLNLTQLPPFNVHLQTKLVLLERLPRPS

Target ORF information:

RefSeq Version	NM_024105.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ALG12, alpha-1,6-mannosyltransferase (ALG12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024105.3

ORF Insert Sequence:

ATGGCTGGAA AGGGGTCATC AGGCAGGCGG CCCCTGCTGC TGGGGCTGCT GGTGGCCGTA 
GCCACTGTCC ACCTGGTCAT CTGTCCCTAC ACCAAAGTGG AGGAGAGCTT CAACCTGCAG 
GCCACACATG ACCTGCTCTA CCACTGGCAA GACCTGGAGC AGTACGACCA TCTTGAGTTC 
CCCGGAGTCG TCCCCAGGAC GTTCCTCGGG CCAGTGGTGA TCGCAGTGTT CTCCAGCCCC 
GCGGTTTACG TGCTTTCGCT GTTAGAAATG TCCAAGTTTT ACTCTCAGCT AATAGTTAGA 
GGAGTGCTTG GACTCGGCGT GATTTTTGGA CTCTGGACGT TACAAAAGGA AGTGAGACGG 
CACTTCGGGG CCATGGTGGC CACCATGTTC TGCTGGGTGA CGGCCATGCA GTTCCACCTG 
ATGTTCTACT GCACGCGGAC ACTGCCCAAT GTGCTGGCCC TGCCTGTAGT CCTGCTGGCC 
CTCGCGGCCT GGCTGCGGCA CGAGTGGGCC CGCTTCATCT GGCTGTCAGC CTTCGCCATC 
ATCGTGTTCA GGGTGGAGCT GTGCCTGTTC CTGGGCCTCC TGCTGCTGCT GGCCTTGGGC 
AACCGAAAGG TTTCTGTAGT CAGAGCCCTT CGCCACGCCG TCCCGGCAGG GATCCTCTGT 
TTAGGACTGA CGGTTGCTGT GGACTCTTAT TTTTGGCGGC AGCTCACTTG GCCGGAAGGA 
AAGGTGCTTT GGTACAACAC TGTCCTGAAC AAAAGCTCCA ACTGGGGGAC CTCCCCGCTG 
CTGTGGTACT TCTACTCAGC CCTGCCCCGC GGCCTGGGCT GCAGCCTGCT CTTCATCCCC 
CTGGGCTTGG TAGACAGAAG GACGCACGCG CCGACGGTGC TGGCACTGGG CTTCATGGCA 
CTCTACTCCC TCCTGCCACA CAAGGAGCTA CGCTTCATCA TCTATGCCTT CCCCATGCTC 
AACATCACGG CTGCCAGAGG CTGCTCCTAC CTGCTGAATA ACTATAAAAA GTCTTGGCTG 
TACAAAGCGG GGTCTCTGCT TGTGATCGGA CACCTCGTGG TGAATGCCGC CTACTCAGCC 
ACGGCCCTGT ATGTGTCCCA TTTCAACTAC CCAGGTGGCG TCGCAATGCA GAGGCTGCAC 
CAGCTGGTGC CCCCCCAGAC AGACGTCCTT CTGCACATTG ACGTGGCAGC CGCCCAGACA 
GGTGTGTCTC GGTTTCTCCA AGTCAACAGC GCCTGGAGGT ACGACAAGAG GGAGGATGTG 
CAGCCGGGGA CAGGCATGCT GGCATACACA CACATCCTCA TGGAGGCGGC CCCTGGGCTC 
CTGGCCCTCT ACAGGGACAC ACACCGGGTC CTGGCCAGCG TCGTGGGGAC CACAGGTGTG 
AGTCTGAACC TGACCCAACT GCCCCCCTTC AACGTCCACC TGCAGACAAA GCTGGTGCTT 
CTGGAGAGGC TCCCCCGGCC GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
Nature communications54020(2014 Jun)
Navarini AA,Simpson MA,Weale M,Knight J,Carlavan I,Reiniche P,Burden DA,Layton A,Bataille V,Allen M,Pleass R,Pink A,Creamer D,English J,Munn S,Walton S,,Willis C,Déret S,Voegel JJ,Spector T,Smith CH,Trembath RC,Barker JN

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Large-scale cDNA transfection screening for genes related to cancer development and progression.
Proceedings of the National Academy of Sciences of the United States of America101(44)15724-9(2004 Nov)
Wan D,Gong Y,Qin W,Zhang P,Li J,Wei L,Zhou X,Li H,Qiu X,Zhong F,He L,Yu J,Yao G,Jiang H,Qian L,Yu Y,Shu H,Chen X,Xu H,Guo M,Pan Z,Chen Y,Ge C,Yang S,Gu J

Congenital disorders of glycosylation: a booming chapter of pediatrics.
Current opinion in pediatrics16(4)434-9(2004 Aug)
Jaeken J,Carchon H

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ALG12 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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