TUFM cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TUFM
Entrez Gene ID	7284
Full Name	Tu translation elongation factor, mitochondrial
Synonyms	COXPD4,EF-TuMT,EFTU,P43
General protein information	Preferred Names Tu translation elongation factor, mitochondrial Names elongation factor Tu, mitochondrial EF-Tu
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p11.2
Summary	This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq, Jul 2008].
Disorder MIM:	602389
Disorder Html:	Combined oxidative phosphorylation deficiency 4, 610678 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011545928.2	XP_011544230.1	elongation factor Tu, mitochondrial isoform X2
XM_017023619.1	XP_016879108.1	elongation factor Tu, mitochondrial isoform X1
NM_003321.4	NP_003312.3	elongation factor Tu, mitochondrial precursor
NM_001365360.1	NP_001352289.1	elongation factor Tu, mitochondrial isoform 2 precursor
NM_001365360.2	NP_001352289.1	elongation factor Tu, mitochondrial isoform 2 precursor
NM_003321.5	NP_003312.3	elongation factor Tu, mitochondrial isoform 1 precursor

Pathways from BioSystems

Homologies

Macaca mulatta (Rhesus monkey)	TUFM	XP_001103519.1
Kluyveromyces lactis	KLLA0A04587g	XP_451201.1
Kluyveromyces lactis	KLLA0C05214g	XP_452433.1
Eremothecium gossypii	AGOS_AAR143W	NP_982685.1
Homo sapiens (human)	TUFM	NP_003312.3
Saccharomyces cerevisiae (baker's yeast)	TUF1	NP_014830.1
Magnaporthe oryzae (rice blast fungus)	MGG_08162	XP_003715132.1
Canis lupus familiaris (dog)	TUFM	XP_536924.3
Bos taurus (cattle)	TUFM	NP_776632.1
Rattus norvegicus (Norway rat)	Tufm	NP_001099765.1
Danio rerio (zebrafish)	tufm	NP_001013523.1
Neurospora crassa	NCU03737	XP_961513.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006996	XP_308774.4
Pan troglodytes (chimpanzee)	TUFM	XP_510904.2
Drosophila melanogaster (fruit fly)	EfTuM	NP_001163144.1
Arabidopsis thaliana (thale cress)	AT4G02930	NP_192202.1
Oryza sativa (rice)	Os03g0851100	NP_001051912.1
Schizosaccharomyces pombe (fission yeast)	tuf1	NP_595746.1
Mus musculus (house mouse)	Tufm	NP_766333.1
Caenorhabditis elegans (roundworm)	tufm-1	NP_497623.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.
Kuo SM, Chen CJ, Chang SC, Liu TJ, Chen YH, Huang SY, Shih SR
mBio8(3)(2017 06)

Novel mutation in mitochondrial Elongation Factor EF-Tu associated to dysplastic leukoencephalopathy and defective mitochondrial DNA translation.
Di Nottia M, Montanari A, Verrigni D, Oliva R, Torraco A, Fernandez-Vizarra E, Diodato D, Rizza T, Bianchi M, Catteruccia M, Zeviani M, Dionisi-Vici C, Francisci S, Bertini E, Carrozzo R
Biochimica et biophysica acta. Molecular basis of disease1863(4)961-967(2017 04)

Expression and clinicopathologic significance of TUFM and p53 for the normal-adenoma-carcinoma sequence in colorectal epithelia.
Xi HQ, Zhang KC, Li JY, Cui JX, Zhao P, Chen L
World journal of surgical oncology15(1)90(2017 Apr)

EGFR-targeted mAb therapy modulates autophagy in head and neck squamous cell carcinoma through NLRX1-TUFM protein complex.
Lei Y, Kansy BA, Li J, Cong L, Liu Y, Trivedi S, Wen H, Ting JP, Ouyang H, Ferris RL
Oncogene35(36)4698-707(2016 09)

TUFM downregulation induces epithelial-mesenchymal transition and invasion in lung cancer cells via a mechanism involving AMPK-GSK3β signaling.
He K, Guo X, Liu Y, Li J, Hu Y, Wang D, Song J
Cellular and molecular life sciences : CMLS73(10)2105-21(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TUFM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TUFM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu61179	NM_001365360.2 Latest version!	Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu61179	XM_011545928.2 Latest version!	Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu18903	NM_003321.5 Latest version!	Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
OHu18903	XM_017023619.1 Latest version!	Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
View Old Accession Versions >>
OHu61179	NM_001365360.1	Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu18903	NM_003321.4	Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TUFM ( NM_001365360.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352289.1 Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu61179
Clone ID Related Accession (Same CDS sequence)	XM_011545928.2 , NM_001365360.1 , NM_001365360.2
Accession Version	NM_001365360.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1542988800000
Organism	Homo sapiens(human)
Product	elongation factor Tu, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC133550.2. On Aug 20, 2018 this sequence version replaced XM_011545928.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.551915.1, SRR1163657.545971.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGC 
ATTGAGATGT TCCACAAGAG CCTGGAGAGG GCCGAGGCCG GAGATAACCT CGGGGCCCTG 
GTCCGAGGCT TGAAGCGGGA GGACTTGCGG CGGGGCCTGG TCATGGTCAA GCCAGGTTCC 
ATCAAGCCCC ACCAGAAGGT GGAGGCCCAG GTTTACATCC TCAGCAAGGA GGAAGGTGGC 
CGCCACAAGC CCTTTGTGTC CCACTTCATG CCTGTCATGT TCTCCCTGAC TTGGGACATG 
GCCTGTCGGA TTATCCTGCC CCCAGAGAAG GAGCTTGCCA TGCCCGGGGA GGACCTGAAG 
TTCAACCTAA TCTTGCGGCA GCCAATGATC TTAGAGAAAG GCCAGCGTTT CACCCTGCGA 
GATGGCAACC GGACTATTGG CACCGGTCTA GTCACCAACA CGCTGGCCAT GACTGAGGAG 
GAGAAGAATA TCAAATGGGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352289.1
CDS	80..1363
Translation	MTTMAAATLLRATPHFSGLAAGRTFLLQGLLRLLKAPALPLLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

Target ORF information:

RefSeq Version	NM_001365360.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365360.1

ORF Insert Sequence:

ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGC 
ATTGAGATGT TCCACAAGAG CCTGGAGAGG GCCGAGGCCG GAGATAACCT CGGGGCCCTG 
GTCCGAGGCT TGAAGCGGGA GGACTTGCGG CGGGGCCTGG TCATGGTCAA GCCAGGTTCC 
ATCAAGCCCC ACCAGAAGGT GGAGGCCCAG GTTTACATCC TCAGCAAGGA GGAAGGTGGC 
CGCCACAAGC CCTTTGTGTC CCACTTCATG CCTGTCATGT TCTCCCTGAC TTGGGACATG 
GCCTGTCGGA TTATCCTGCC CCCAGAGAAG GAGCTTGCCA TGCCCGGGGA GGACCTGAAG 
TTCAACCTAA TCTTGCGGCA GCCAATGATC TTAGAGAAAG GCCAGCGTTT CACCCTGCGA 
GATGGCAACC GGACTATTGG CACCGGTCTA GTCACCAACA CGCTGGCCAT GACTGAGGAG 
GAGAAGAATA TCAAATGGGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TUFM ( NM_001365360.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001352289.1 Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu61179
Clone ID Related Accession (Same CDS sequence)	XM_011545928.2 , NM_001365360.1 , NM_001365360.2
Accession Version	NM_001365360.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577980800000
Organism	Homo sapiens(human)
Product	elongation factor Tu, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC133550.2. On May 31, 2019 this sequence version replaced NM_001365360.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.551915.1, SRR5189667.403990.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGC 
ATTGAGATGT TCCACAAGAG CCTGGAGAGG GCCGAGGCCG GAGATAACCT CGGGGCCCTG 
GTCCGAGGCT TGAAGCGGGA GGACTTGCGG CGGGGCCTGG TCATGGTCAA GCCAGGTTCC 
ATCAAGCCCC ACCAGAAGGT GGAGGCCCAG GTTTACATCC TCAGCAAGGA GGAAGGTGGC 
CGCCACAAGC CCTTTGTGTC CCACTTCATG CCTGTCATGT TCTCCCTGAC TTGGGACATG 
GCCTGTCGGA TTATCCTGCC CCCAGAGAAG GAGCTTGCCA TGCCCGGGGA GGACCTGAAG 
TTCAACCTAA TCTTGCGGCA GCCAATGATC TTAGAGAAAG GCCAGCGTTT CACCCTGCGA 
GATGGCAACC GGACTATTGG CACCGGTCTA GTCACCAACA CGCTGGCCAT GACTGAGGAG 
GAGAAGAATA TCAAATGGGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352289.1
CDS	80..1363
Translation	MTTMAAATLLRATPHFSGLAAGRTFLLQGLLRLLKAPALPLLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

Target ORF information:

RefSeq Version	NM_001365360.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365360.2

ORF Insert Sequence:

ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGC 
ATTGAGATGT TCCACAAGAG CCTGGAGAGG GCCGAGGCCG GAGATAACCT CGGGGCCCTG 
GTCCGAGGCT TGAAGCGGGA GGACTTGCGG CGGGGCCTGG TCATGGTCAA GCCAGGTTCC 
ATCAAGCCCC ACCAGAAGGT GGAGGCCCAG GTTTACATCC TCAGCAAGGA GGAAGGTGGC 
CGCCACAAGC CCTTTGTGTC CCACTTCATG CCTGTCATGT TCTCCCTGAC TTGGGACATG 
GCCTGTCGGA TTATCCTGCC CCCAGAGAAG GAGCTTGCCA TGCCCGGGGA GGACCTGAAG 
TTCAACCTAA TCTTGCGGCA GCCAATGATC TTAGAGAAAG GCCAGCGTTT CACCCTGCGA 
GATGGCAACC GGACTATTGG CACCGGTCTA GTCACCAACA CGCTGGCCAT GACTGAGGAG 
GAGAAGAATA TCAAATGGGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TUFM ( XM_011545928.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011544230.1 Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu61179
Clone ID Related Accession (Same CDS sequence)	XM_011545928.2 , NM_001365360.1 , NM_001365360.2
Accession Version	XM_011545928.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	elongation factor Tu, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545928.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGC 
ATTGAGATGT TCCACAAGAG CCTGGAGAGG GCCGAGGCCG GAGATAACCT CGGGGCCCTG 
GTCCGAGGCT TGAAGCGGGA GGACTTGCGG CGGGGCCTGG TCATGGTCAA GCCAGGTTCC 
ATCAAGCCCC ACCAGAAGGT GGAGGCCCAG GTTTACATCC TCAGCAAGGA GGAAGGTGGC 
CGCCACAAGC CCTTTGTGTC CCACTTCATG CCTGTCATGT TCTCCCTGAC TTGGGACATG 
GCCTGTCGGA TTATCCTGCC CCCAGAGAAG GAGCTTGCCA TGCCCGGGGA GGACCTGAAG 
TTCAACCTAA TCTTGCGGCA GCCAATGATC TTAGAGAAAG GCCAGCGTTT CACCCTGCGA 
GATGGCAACC GGACTATTGG CACCGGTCTA GTCACCAACA CGCTGGCCAT GACTGAGGAG 
GAGAAGAATA TCAAATGGGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011544230.1
CDS	234..1517
Translation	MTTMAAATLLRATPHFSGLAAGRTFLLQGLLRLLKAPALPLLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

Target ORF information:

RefSeq Version	XM_011545928.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545928.2

ORF Insert Sequence:

ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGC 
ATTGAGATGT TCCACAAGAG CCTGGAGAGG GCCGAGGCCG GAGATAACCT CGGGGCCCTG 
GTCCGAGGCT TGAAGCGGGA GGACTTGCGG CGGGGCCTGG TCATGGTCAA GCCAGGTTCC 
ATCAAGCCCC ACCAGAAGGT GGAGGCCCAG GTTTACATCC TCAGCAAGGA GGAAGGTGGC 
CGCCACAAGC CCTTTGTGTC CCACTTCATG CCTGTCATGT TCTCCCTGAC TTGGGACATG 
GCCTGTCGGA TTATCCTGCC CCCAGAGAAG GAGCTTGCCA TGCCCGGGGA GGACCTGAAG 
TTCAACCTAA TCTTGCGGCA GCCAATGATC TTAGAGAAAG GCCAGCGTTT CACCCTGCGA 
GATGGCAACC GGACTATTGG CACCGGTCTA GTCACCAACA CGCTGGCCAT GACTGAGGAG 
GAGAAGAATA TCAAATGGGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TUFM ( NM_003321.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003312.3 Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), mRNA.

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CloneID	OHu18903
Clone ID Related Accession (Same CDS sequence)	XM_017023619.1 , NM_003321.4 , NM_003321.5
Accession Version	NM_003321.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1510329600000
Organism	Homo sapiens(human)
Product	elongation factor Tu, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC133550.2, BC010041.2 and CA420396.1. This sequence is a reference standard in the RefSeqGene project. On Mar 7, 2008 this sequence version replaced NM_003321.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.251890.1, SRR1803612.72950.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGT 
ACACTAGAGC GTGGCATTTT AAAGAAGGGA GACGAGTGTG AGCTCCTAGG ACATAGCAAG 
AACATCCGCA CTGTGGTGAC AGGCATTGAG ATGTTCCACA AGAGCCTGGA GAGGGCCGAG 
GCCGGAGATA ACCTCGGGGC CCTGGTCCGA GGCTTGAAGC GGGAGGACTT GCGGCGGGGC 
CTGGTCATGG TCAAGCCAGG TTCCATCAAG CCCCACCAGA AGGTGGAGGC CCAGGTTTAC 
ATCCTCAGCA AGGAGGAAGG TGGCCGCCAC AAGCCCTTTG TGTCCCACTT CATGCCTGTC 
ATGTTCTCCC TGACTTGGGA CATGGCCTGT CGGATTATCC TGCCCCCAGA GAAGGAGCTT 
GCCATGCCCG GGGAGGACCT GAAGTTCAAC CTAATCTTGC GGCAGCCAAT GATCTTAGAG 
AAAGGCCAGC GTTTCACCCT GCGAGATGGC AACCGGACTA TTGGCACCGG TCTAGTCACC 
AACACGCTGG CCATGACTGA GGAGGAGAAG AATATCAAAT GGGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003312.3
CDS	140..1507
Translation	MTTMAAATLLRATPHFSGLAAGRTFLLQGLLRLLKAPALPLLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

Target ORF information:

RefSeq Version	NM_003321.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003321.4

ORF Insert Sequence:

ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGT 
ACACTAGAGC GTGGCATTTT AAAGAAGGGA GACGAGTGTG AGCTCCTAGG ACATAGCAAG 
AACATCCGCA CTGTGGTGAC AGGCATTGAG ATGTTCCACA AGAGCCTGGA GAGGGCCGAG 
GCCGGAGATA ACCTCGGGGC CCTGGTCCGA GGCTTGAAGC GGGAGGACTT GCGGCGGGGC 
CTGGTCATGG TCAAGCCAGG TTCCATCAAG CCCCACCAGA AGGTGGAGGC CCAGGTTTAC 
ATCCTCAGCA AGGAGGAAGG TGGCCGCCAC AAGCCCTTTG TGTCCCACTT CATGCCTGTC 
ATGTTCTCCC TGACTTGGGA CATGGCCTGT CGGATTATCC TGCCCCCAGA GAAGGAGCTT 
GCCATGCCCG GGGAGGACCT GAAGTTCAAC CTAATCTTGC GGCAGCCAAT GATCTTAGAG 
AAAGGCCAGC GTTTCACCCT GCGAGATGGC AACCGGACTA TTGGCACCGG TCTAGTCACC 
AACACGCTGG CCATGACTGA GGAGGAGAAG AATATCAAAT GGGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TUFM ( NM_003321.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003312.3 Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 1, mRNA.

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CloneID	OHu18903
Clone ID Related Accession (Same CDS sequence)	XM_017023619.1 , NM_003321.4 , NM_003321.5
Accession Version	NM_003321.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577635200000
Organism	Homo sapiens(human)
Product	elongation factor Tu, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC133550.2, BC010041.2 and CA420396.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_003321.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.291522.1, SRR1803613.251890.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145313 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000313511.8/ ENSP00000322439.3 RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGT 
ACACTAGAGC GTGGCATTTT AAAGAAGGGA GACGAGTGTG AGCTCCTAGG ACATAGCAAG 
AACATCCGCA CTGTGGTGAC AGGCATTGAG ATGTTCCACA AGAGCCTGGA GAGGGCCGAG 
GCCGGAGATA ACCTCGGGGC CCTGGTCCGA GGCTTGAAGC GGGAGGACTT GCGGCGGGGC 
CTGGTCATGG TCAAGCCAGG TTCCATCAAG CCCCACCAGA AGGTGGAGGC CCAGGTTTAC 
ATCCTCAGCA AGGAGGAAGG TGGCCGCCAC AAGCCCTTTG TGTCCCACTT CATGCCTGTC 
ATGTTCTCCC TGACTTGGGA CATGGCCTGT CGGATTATCC TGCCCCCAGA GAAGGAGCTT 
GCCATGCCCG GGGAGGACCT GAAGTTCAAC CTAATCTTGC GGCAGCCAAT GATCTTAGAG 
AAAGGCCAGC GTTTCACCCT GCGAGATGGC AACCGGACTA TTGGCACCGG TCTAGTCACC 
AACACGCTGG CCATGACTGA GGAGGAGAAG AATATCAAAT GGGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003312.3
CDS	80..1447
Translation	MTTMAAATLLRATPHFSGLAAGRTFLLQGLLRLLKAPALPLLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

Target ORF information:

RefSeq Version	NM_003321.5
Organism	Homo sapiens(human)
Definition	Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003321.5

ORF Insert Sequence:

ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGT 
ACACTAGAGC GTGGCATTTT AAAGAAGGGA GACGAGTGTG AGCTCCTAGG ACATAGCAAG 
AACATCCGCA CTGTGGTGAC AGGCATTGAG ATGTTCCACA AGAGCCTGGA GAGGGCCGAG 
GCCGGAGATA ACCTCGGGGC CCTGGTCCGA GGCTTGAAGC GGGAGGACTT GCGGCGGGGC 
CTGGTCATGG TCAAGCCAGG TTCCATCAAG CCCCACCAGA AGGTGGAGGC CCAGGTTTAC 
ATCCTCAGCA AGGAGGAAGG TGGCCGCCAC AAGCCCTTTG TGTCCCACTT CATGCCTGTC 
ATGTTCTCCC TGACTTGGGA CATGGCCTGT CGGATTATCC TGCCCCCAGA GAAGGAGCTT 
GCCATGCCCG GGGAGGACCT GAAGTTCAAC CTAATCTTGC GGCAGCCAAT GATCTTAGAG 
AAAGGCCAGC GTTTCACCCT GCGAGATGGC AACCGGACTA TTGGCACCGG TCTAGTCACC 
AACACGCTGG CCATGACTGA GGAGGAGAAG AATATCAAAT GGGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TUFM ( XM_017023619.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016879108.1 Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu18903
Clone ID Related Accession (Same CDS sequence)	XM_017023619.1 , NM_003321.4 , NM_003321.5
Accession Version	XM_017023619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1368bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	elongation factor Tu, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGT 
ACACTAGAGC GTGGCATTTT AAAGAAGGGA GACGAGTGTG AGCTCCTAGG ACATAGCAAG 
AACATCCGCA CTGTGGTGAC AGGCATTGAG ATGTTCCACA AGAGCCTGGA GAGGGCCGAG 
GCCGGAGATA ACCTCGGGGC CCTGGTCCGA GGCTTGAAGC GGGAGGACTT GCGGCGGGGC 
CTGGTCATGG TCAAGCCAGG TTCCATCAAG CCCCACCAGA AGGTGGAGGC CCAGGTTTAC 
ATCCTCAGCA AGGAGGAAGG TGGCCGCCAC AAGCCCTTTG TGTCCCACTT CATGCCTGTC 
ATGTTCTCCC TGACTTGGGA CATGGCCTGT CGGATTATCC TGCCCCCAGA GAAGGAGCTT 
GCCATGCCCG GGGAGGACCT GAAGTTCAAC CTAATCTTGC GGCAGCCAAT GATCTTAGAG 
AAAGGCCAGC GTTTCACCCT GCGAGATGGC AACCGGACTA TTGGCACCGG TCTAGTCACC 
AACACGCTGG CCATGACTGA GGAGGAGAAG AATATCAAAT GGGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016879108.1
CDS	397..1764
Translation	MTTMAAATLLRATPHFSGLAAGRTFLLQGLLRLLKAPALPLLCRGLAVEAKKTYVRDKPHVNVGTIGHVDHGKTTLTAAITKILAEGGGAKFKKYEEIDNAPEERARGITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

Target ORF information:

RefSeq Version	XM_017023619.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Tu translation elongation factor, mitochondrial (TUFM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023619.1

ORF Insert Sequence:

ATGACCACAA TGGCGGCCGC CACCCTGCTG CGCGCGACGC CCCACTTCAG CGGTCTCGCC 
GCCGGCCGGA CCTTCCTGCT GCAGGGTCTG TTGCGGCTGC TGAAAGCCCC GGCATTGCCT 
CTCTTGTGCC GCGGCCTGGC CGTGGAGGCC AAGAAGACTT ACGTGCGCGA CAAGCCACAT 
GTGAATGTGG GTACCATCGG CCATGTGGAC CACGGGAAGA CCACGCTGAC TGCAGCCATC 
ACGAAGATTC TAGCTGAGGG AGGTGGGGCT AAGTTCAAGA AGTACGAGGA GATTGACAAT 
GCCCCGGAGG AGCGAGCTCG GGGTATCACC ATCAATGCGG CTCATGTGGA GTATAGCACT 
GCCGCCCGCC ACTACGCCCA CACAGACTGC CCGGGTCATG CAGATTATGT TAAGAATATG 
ATCACAGGCA CTGCACCCCT CGACGGCTGC ATCCTGGTGG TAGCAGCCAA TGACGGCCCC 
ATGCCCCAGA CCCGAGAGCA CTTATTACTG GCCAGACAGA TTGGGGTGGA GCATGTGGTG 
GTGTATGTGA ACAAGGCTGA CGCTGTCCAG GACTCTGAGA TGGTGGAACT GGTGGAACTG 
GAGATCCGGG AGCTGCTCAC CGAGTTTGGC TATAAAGGGG AGGAGACCCC AGTCATCGTA 
GGCTCTGCTC TCTGTGCCCT TGAGGGTCGG GACCCTGAGT TAGGCCTGAA GTCTGTGCAG 
AAGCTACTGG ATGCTGTGGA CACTTACATC CCAGTGCCCG CCCGGGACCT GGAGAAGCCT 
TTCCTGCTGC CTGTGGAGGC GGTGTACTCC GTCCCTGGCC GTGGCACCGT GGTGACAGGT 
ACACTAGAGC GTGGCATTTT AAAGAAGGGA GACGAGTGTG AGCTCCTAGG ACATAGCAAG 
AACATCCGCA CTGTGGTGAC AGGCATTGAG ATGTTCCACA AGAGCCTGGA GAGGGCCGAG 
GCCGGAGATA ACCTCGGGGC CCTGGTCCGA GGCTTGAAGC GGGAGGACTT GCGGCGGGGC 
CTGGTCATGG TCAAGCCAGG TTCCATCAAG CCCCACCAGA AGGTGGAGGC CCAGGTTTAC 
ATCCTCAGCA AGGAGGAAGG TGGCCGCCAC AAGCCCTTTG TGTCCCACTT CATGCCTGTC 
ATGTTCTCCC TGACTTGGGA CATGGCCTGT CGGATTATCC TGCCCCCAGA GAAGGAGCTT 
GCCATGCCCG GGGAGGACCT GAAGTTCAAC CTAATCTTGC GGCAGCCAAT GATCTTAGAG 
AAAGGCCAGC GTTTCACCCT GCGAGATGGC AACCGGACTA TTGGCACCGG TCTAGTCACC 
AACACGCTGG CCATGACTGA GGAGGAGAAG AATATCAAAT GGGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.
mBio8(3)(2017 06)
Kuo SM,Chen CJ,Chang SC,Liu TJ,Chen YH,Huang SY,Shih SR

Novel mutation in mitochondrial Elongation Factor EF-Tu associated to dysplastic leukoencephalopathy and defective mitochondrial DNA translation.
Biochimica et biophysica acta. Molecular basis of disease1863(4)961-967(2017 04)
Di Nottia M,Montanari A,Verrigni D,Oliva R,Torraco A,Fernandez-Vizarra E,Diodato D,Rizza T,Bianchi M,Catteruccia M,Zeviani M,Dionisi-Vici C,Francisci S,Bertini E,Carrozzo R

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TUFM cDNA ORF clone, Homo sapiens(human)

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