TBXT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TBXT
Entrez Gene ID	6862
Full Name	T-box transcription factor T
Synonyms	SAVA,T,TFT
General protein information	Preferred Names T-box transcription factor T Names brachyury protein T brachyury transcription factor T, brachyury homolog protein T
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q27
Summary	The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012].
Disorder MIM:	601397
Disorder Html:	{Neural tube defects, susceptibility to}, 182940 (3); Sacral agenesis with vertebral anomalies, 615709 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011536080.2	XP_011534382.1	brachyury protein isoform X1
NM_001366285.1	NP_001353214.1	T-box transcription factor T isoform 3
NM_001366286.1	NP_001353215.1	T-box transcription factor T isoform 3
NM_003181.3	NP_003172.1	T-box transcription factor T isoform 1
NM_001270484.1	NP_001257413.1	T-box transcription factor T isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	T	NP_001003092.1
Rattus norvegicus (Norway rat)	T	NP_001099679.1
Gallus gallus (chicken)	T	NP_990271.1
Danio rerio (zebrafish)	LOC100004296	XP_001343633.3
Mus musculus (house mouse)	T	NP_033335.1
Homo sapiens (human)	T	NP_003172.1
Macaca mulatta (Rhesus monkey)	T	XP_001101514.1
Pan troglodytes (chimpanzee)	T	XP_527563.3
Bos taurus (cattle)	T	NP_001179914.1
Xenopus tropicalis (tropical clawed frog)	t	NP_001008139.1

Gene Ontology
Bibliography

Related articles in PubMed

Transfection of T-Box Transcription Factor
Akimoto N, Nakamura K, Hijioka H, Kume K, Matsumura Y, Sugiura T
International journal of molecular sciences19(11)(2018 Nov)

Brachyury oncogene is a prognostic factor in high-risk testicular germ cell tumors.
Pinto F, Cárcano FM, da Silva ECA, Vidal DO, Scapulatempo-Neto C, Lopes LF, Reis RM
Andrology6(4)597-604(2018 07)

Brachyury gene copy number gain and activation of the PI3K/Akt pathway: association with upregulation of oncogenic Brachyury expression in skull base chordoma.
Otani R, Mukasa A, Shin M, Omata M, Takayanagi S, Tanaka S, Ueki K, Saito N
Journal of neurosurgery128(5)1428-1437(2018 05)

The lipodystrophic hotspot lamin A p.R482W mutation deregulates the mesodermal inducer T/Brachyury and early vascular differentiation gene networks.
Briand N, Guénantin AC, Jeziorowska D, Shah A, Mantecon M, Capel E, Garcia M, Oldenburg A, Paulsen J, Hulot JS, Vigouroux C, Collas P
Human molecular genetics27(8)1447-1459(2018 04)

The driver landscape of sporadic chordoma.
Tarpey PS, Behjati S, Young MD, Martincorena I, Alexandrov LB, Farndon SJ, Guzzo C, Hardy C, Latimer C, Butler AP, Teague JW, Shlien A, Futreal PA, Shah S, Bashashati A, Jamshidi F, Nielsen TO, Huntsman D, Baumhoer D, Brandner S, Wunder J, Dickson B, Cogswell P, Sommer J, Phillips JJ, Amary MF, Tirabosco R, Pillay N, Yip S, Stratton MR, Flanagan AM, Campbell PJ
Nature communications8(1)890(2017 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TBXT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBXT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17747	NM_001270484.1 Latest version!	Homo sapiens T-box transcription factor T (TBXT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu60837	NM_001366285.1 Latest version!	Homo sapiens T-box transcription factor T (TBXT), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu60837	NM_001366286.1 Latest version!	Homo sapiens T-box transcription factor T (TBXT), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu60837	XM_011536080.2 Latest version!	Homo sapiens T brachyury transcription factor (T), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu17741	NM_003181.3 Latest version!	Homo sapiens T-box transcription factor T (TBXT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$251.30 $359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TBXT ( NM_001270484.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257413.1 Homo sapiens T-box transcription factor T (TBXT), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17747
Clone ID Related Accession (Same CDS sequence)	NM_001270484.1
Accession Version	NM_001270484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568476800000
Organism	Homo sapiens(human)
Product	T-box transcription factor T isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE785653.1, BC098425.1 and AL627443.6. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098425.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2158188, SAMEA2161674 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC 
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT 
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT 
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC 
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC 
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC 
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG 
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC 
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC 
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA 
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG 
TACTCCCAAT CCTATTCTGA CAACTCACCT GCATGTTTAT CCATGCTGCA ATCCCATGAC 
AATTGGTCCA GCCTTGGAAT GCCTGCCCAT CCCAGCATGC TCCCCGTGAG CCACAATGCC 
AGCCCACCTA CCAGCTCCAG TCAGTACCCC AGCCTGTGGT CTGTGAGCAA CGGCGCCGTC 
ACCCCGGGCT CCCAGGCAGC AGCCGTGTCC AACGGGCTGG GGGCCCAGTT CTTCCGGGGC 
TCCCCCGCGC ACTACACACC CCTCACCCAT CCGGTCTCGG CGCCCTCTTC CTCGGGATCC 
CCACTGTACG AAGGGGCGGC CGCGGCCACA GACATCGTGG ACAGCCAGTA CGACGCCGCA 
GCCCAAGGCC GCCTCATAGC CTCATGGACA CCTGTGTCGC CACCTTCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257413.1
CDS	427..1560
Translation	MSSPGTESAGKSLQYRVDHLLSAVENELQAGSEKGDPTERELRVGLEESELWLRFKELTNEMIVTKNGRRMFPVLKVNVSGLDPNAMYSFLLDFVAADNHRWKYVNGEWVPGGKPEPQAPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPQRMITSHCFPETQFIAVTAYQNEEITALKIKYNPFAKAFLDAKERSDHKEMMEEPGDSQQPGYSQSYSDNSPACLSMLQSHDNWSSLGMPAHPSMLPVSHNASPPTSSSQYPSLWSVSNGAVTPGSQAAAVSNGLGAQFFRGSPAHYTPLTHPVSAPSSSGSPLYEGAAAATDIVDSQYDAAAQGRLIASWTPVSPPSM

Target ORF information:

RefSeq Version	NM_001270484.1
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box transcription factor T (TBXT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270484.1

ORF Insert Sequence:

ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC 
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT 
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT 
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC 
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC 
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC 
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG 
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC 
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC 
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA 
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG 
TACTCCCAAT CCTATTCTGA CAACTCACCT GCATGTTTAT CCATGCTGCA ATCCCATGAC 
AATTGGTCCA GCCTTGGAAT GCCTGCCCAT CCCAGCATGC TCCCCGTGAG CCACAATGCC 
AGCCCACCTA CCAGCTCCAG TCAGTACCCC AGCCTGTGGT CTGTGAGCAA CGGCGCCGTC 
ACCCCGGGCT CCCAGGCAGC AGCCGTGTCC AACGGGCTGG GGGCCCAGTT CTTCCGGGGC 
TCCCCCGCGC ACTACACACC CCTCACCCAT CCGGTCTCGG CGCCCTCTTC CTCGGGATCC 
CCACTGTACG AAGGGGCGGC CGCGGCCACA GACATCGTGG ACAGCCAGTA CGACGCCGCA 
GCCCAAGGCC GCCTCATAGC CTCATGGACA CCTGTGTCGC CACCTTCCAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBXT ( NM_001366285.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353214.1 Homo sapiens T-box transcription factor T (TBXT), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu60837
Clone ID Related Accession (Same CDS sequence)	XM_011536080.2 , NM_001366285.1 , NM_001366286.1
Accession Version	NM_001366285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568476800000
Organism	Homo sapiens(human)
Product	T-box transcription factor T isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627443.6. On Sep 28, 2018 this sequence version replaced XM_011536080.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC039877.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC 
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT 
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT 
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC 
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC 
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC 
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG 
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC 
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC 
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA 
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG 
TACTCCCAAT CAGGGGGGTG GCTTCTTCCT GGAACCAGCA CCCTGTGTCC ACCTGCAAAT 
CCTCATCCTC AGTTTGGAGG TGCCCTCTCC CTCCCCTCCA CGCACAGCTG TGACAGGTAC 
CCAACCCTGA GGAGCCACCG GTCCTCACCC TACCCCAGCC CCTATGCTCA TCGGAACAAT 
TCTCCAACCT ATTCTGACAA CTCACCTGCA TGTTTATCCA TGCTGCAATC CCATGACAAT 
TGGTCCAGCC TTGGAATGCC TGCCCATCCC AGCATGCTCC CCGTGAGCCA CAATGCCAGC 
CCACCTACCA GCTCCAGTCA GTACCCCAGC CTGTGGTCTG TGAGCAACGG CGCCGTCACC 
CCGGGCTCCC AGGCAGCAGC CGTGTCCAAC GGGCTGGGGG CCCAGTTCTT CCGGGGCTCC 
CCCGCGCACT ACACACCCCT CACCCATCCG GTCTCGGCGC CCTCTTCCTC GGGATCCCCA 
CTGTACGAAG GGGCGGCCGC GGCCACAGAC ATCGTGGACA GCCAGTACGA CGCCGCAGCC 
CAAGGCCGCC TCATAGCCTC ATGGACACCT GTGTCGCCAC CTTCCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353214.1
CDS	261..1571
Translation	MSSPGTESAGKSLQYRVDHLLSAVENELQAGSEKGDPTERELRVGLEESELWLRFKELTNEMIVTKNGRRMFPVLKVNVSGLDPNAMYSFLLDFVAADNHRWKYVNGEWVPGGKPEPQAPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPQRMITSHCFPETQFIAVTAYQNEEITALKIKYNPFAKAFLDAKERSDHKEMMEEPGDSQQPGYSQSGGWLLPGTSTLCPPANPHPQFGGALSLPSTHSCDRYPTLRSHRSSPYPSPYAHRNNSPTYSDNSPACLSMLQSHDNWSSLGMPAHPSMLPVSHNASPPTSSSQYPSLWSVSNGAVTPGSQAAAVSNGLGAQFFRGSPAHYTPLTHPVSAPSSSGSPLYEGAAAATDIVDSQYDAAAQGRLIASWTPVSPPSM

Target ORF information:

RefSeq Version	NM_001366285.1
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box transcription factor T (TBXT), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366285.1

ORF Insert Sequence:

ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC 
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT 
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT 
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC 
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC 
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC 
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG 
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC 
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC 
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA 
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG 
TACTCCCAAT CAGGGGGGTG GCTTCTTCCT GGAACCAGCA CCCTGTGTCC ACCTGCAAAT 
CCTCATCCTC AGTTTGGAGG TGCCCTCTCC CTCCCCTCCA CGCACAGCTG TGACAGGTAC 
CCAACCCTGA GGAGCCACCG GTCCTCACCC TACCCCAGCC CCTATGCTCA TCGGAACAAT 
TCTCCAACCT ATTCTGACAA CTCACCTGCA TGTTTATCCA TGCTGCAATC CCATGACAAT 
TGGTCCAGCC TTGGAATGCC TGCCCATCCC AGCATGCTCC CCGTGAGCCA CAATGCCAGC 
CCACCTACCA GCTCCAGTCA GTACCCCAGC CTGTGGTCTG TGAGCAACGG CGCCGTCACC 
CCGGGCTCCC AGGCAGCAGC CGTGTCCAAC GGGCTGGGGG CCCAGTTCTT CCGGGGCTCC 
CCCGCGCACT ACACACCCCT CACCCATCCG GTCTCGGCGC CCTCTTCCTC GGGATCCCCA 
CTGTACGAAG GGGCGGCCGC GGCCACAGAC ATCGTGGACA GCCAGTACGA CGCCGCAGCC 
CAAGGCCGCC TCATAGCCTC ATGGACACCT GTGTCGCCAC CTTCCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBXT ( NM_001366286.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353215.1 Homo sapiens T-box transcription factor T (TBXT), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu60837
Clone ID Related Accession (Same CDS sequence)	XM_011536080.2 , NM_001366285.1 , NM_001366286.1
Accession Version	NM_001366286.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568476800000
Organism	Homo sapiens(human)
Product	T-box transcription factor T isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL627443.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.565062.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC 
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT 
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT 
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC 
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC 
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC 
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG 
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC 
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC 
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA 
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG 
TACTCCCAAT CAGGGGGGTG GCTTCTTCCT GGAACCAGCA CCCTGTGTCC ACCTGCAAAT 
CCTCATCCTC AGTTTGGAGG TGCCCTCTCC CTCCCCTCCA CGCACAGCTG TGACAGGTAC 
CCAACCCTGA GGAGCCACCG GTCCTCACCC TACCCCAGCC CCTATGCTCA TCGGAACAAT 
TCTCCAACCT ATTCTGACAA CTCACCTGCA TGTTTATCCA TGCTGCAATC CCATGACAAT 
TGGTCCAGCC TTGGAATGCC TGCCCATCCC AGCATGCTCC CCGTGAGCCA CAATGCCAGC 
CCACCTACCA GCTCCAGTCA GTACCCCAGC CTGTGGTCTG TGAGCAACGG CGCCGTCACC 
CCGGGCTCCC AGGCAGCAGC CGTGTCCAAC GGGCTGGGGG CCCAGTTCTT CCGGGGCTCC 
CCCGCGCACT ACACACCCCT CACCCATCCG GTCTCGGCGC CCTCTTCCTC GGGATCCCCA 
CTGTACGAAG GGGCGGCCGC GGCCACAGAC ATCGTGGACA GCCAGTACGA CGCCGCAGCC 
CAAGGCCGCC TCATAGCCTC ATGGACACCT GTGTCGCCAC CTTCCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353215.1
CDS	413..1723
Translation	MSSPGTESAGKSLQYRVDHLLSAVENELQAGSEKGDPTERELRVGLEESELWLRFKELTNEMIVTKNGRRMFPVLKVNVSGLDPNAMYSFLLDFVAADNHRWKYVNGEWVPGGKPEPQAPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPQRMITSHCFPETQFIAVTAYQNEEITALKIKYNPFAKAFLDAKERSDHKEMMEEPGDSQQPGYSQSGGWLLPGTSTLCPPANPHPQFGGALSLPSTHSCDRYPTLRSHRSSPYPSPYAHRNNSPTYSDNSPACLSMLQSHDNWSSLGMPAHPSMLPVSHNASPPTSSSQYPSLWSVSNGAVTPGSQAAAVSNGLGAQFFRGSPAHYTPLTHPVSAPSSSGSPLYEGAAAATDIVDSQYDAAAQGRLIASWTPVSPPSM

Target ORF information:

RefSeq Version	NM_001366286.1
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box transcription factor T (TBXT), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366286.1

ORF Insert Sequence:

ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC 
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT 
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT 
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC 
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC 
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC 
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG 
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC 
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC 
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA 
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG 
TACTCCCAAT CAGGGGGGTG GCTTCTTCCT GGAACCAGCA CCCTGTGTCC ACCTGCAAAT 
CCTCATCCTC AGTTTGGAGG TGCCCTCTCC CTCCCCTCCA CGCACAGCTG TGACAGGTAC 
CCAACCCTGA GGAGCCACCG GTCCTCACCC TACCCCAGCC CCTATGCTCA TCGGAACAAT 
TCTCCAACCT ATTCTGACAA CTCACCTGCA TGTTTATCCA TGCTGCAATC CCATGACAAT 
TGGTCCAGCC TTGGAATGCC TGCCCATCCC AGCATGCTCC CCGTGAGCCA CAATGCCAGC 
CCACCTACCA GCTCCAGTCA GTACCCCAGC CTGTGGTCTG TGAGCAACGG CGCCGTCACC 
CCGGGCTCCC AGGCAGCAGC CGTGTCCAAC GGGCTGGGGG CCCAGTTCTT CCGGGGCTCC 
CCCGCGCACT ACACACCCCT CACCCATCCG GTCTCGGCGC CCTCTTCCTC GGGATCCCCA 
CTGTACGAAG GGGCGGCCGC GGCCACAGAC ATCGTGGACA GCCAGTACGA CGCCGCAGCC 
CAAGGCCGCC TCATAGCCTC ATGGACACCT GTGTCGCCAC CTTCCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBXT ( XM_011536080.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534382.1 Homo sapiens T brachyury transcription factor (T), transcript variant X1, mRNA.

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CloneID	OHu60837
Clone ID Related Accession (Same CDS sequence)	XM_011536080.2 , NM_001366285.1 , NM_001366286.1
Accession Version	XM_011536080.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1311bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1465056000000
Organism	Homo sapiens(human)
Product	brachyury protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC 
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT 
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT 
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC 
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC 
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC 
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG 
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC 
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC 
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA 
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG 
TACTCCCAAT CAGGGGGGTG GCTTCTTCCT GGAACCAGCA CCCTGTGTCC ACCTGCAAAT 
CCTCATCCTC AGTTTGGAGG TGCCCTCTCC CTCCCCTCCA CGCACAGCTG TGACAGGTAC 
CCAACCCTGA GGAGCCACCG GTCCTCACCC TACCCCAGCC CCTATGCTCA TCGGAACAAT 
TCTCCAACCT ATTCTGACAA CTCACCTGCA TGTTTATCCA TGCTGCAATC CCATGACAAT 
TGGTCCAGCC TTGGAATGCC TGCCCATCCC AGCATGCTCC CCGTGAGCCA CAATGCCAGC 
CCACCTACCA GCTCCAGTCA GTACCCCAGC CTGTGGTCTG TGAGCAACGG CGCCGTCACC 
CCGGGCTCCC AGGCAGCAGC CGTGTCCAAC GGGCTGGGGG CCCAGTTCTT CCGGGGCTCC 
CCCGCGCACT ACACACCCCT CACCCATCCG GTCTCGGCGC CCTCTTCCTC GGGATCCCCA 
CTGTACGAAG GGGCGGCCGC GGCCACAGAC ATCGTGGACA GCCAGTACGA CGCCGCAGCC 
CAAGGCCGCC TCATAGCCTC ATGGACACCT GTGTCGCCAC CTTCCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534382.1
CDS	287..1597
Translation	MSSPGTESAGKSLQYRVDHLLSAVENELQAGSEKGDPTERELRVGLEESELWLRFKELTNEMIVTKNGRRMFPVLKVNVSGLDPNAMYSFLLDFVAADNHRWKYVNGEWVPGGKPEPQAPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPQRMITSHCFPETQFIAVTAYQNEEITALKIKYNPFAKAFLDAKERSDHKEMMEEPGDSQQPGYSQSGGWLLPGTSTLCPPANPHPQFGGALSLPSTHSCDRYPTLRSHRSSPYPSPYAHRNNSPTYSDNSPACLSMLQSHDNWSSLGMPAHPSMLPVSHNASPPTSSSQYPSLWSVSNGAVTPGSQAAAVSNGLGAQFFRGSPAHYTPLTHPVSAPSSSGSPLYEGAAAATDIVDSQYDAAAQGRLIASWTPVSPPSM

Target ORF information:

RefSeq Version	XM_011536080.2
Organism	Homo sapiens(human)
Definition	Homo sapiens T brachyury transcription factor (T), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536080.2

ORF Insert Sequence:

ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC 
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT 
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT 
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC 
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC 
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC 
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG 
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC 
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC 
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA 
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG 
TACTCCCAAT CAGGGGGGTG GCTTCTTCCT GGAACCAGCA CCCTGTGTCC ACCTGCAAAT 
CCTCATCCTC AGTTTGGAGG TGCCCTCTCC CTCCCCTCCA CGCACAGCTG TGACAGGTAC 
CCAACCCTGA GGAGCCACCG GTCCTCACCC TACCCCAGCC CCTATGCTCA TCGGAACAAT 
TCTCCAACCT ATTCTGACAA CTCACCTGCA TGTTTATCCA TGCTGCAATC CCATGACAAT 
TGGTCCAGCC TTGGAATGCC TGCCCATCCC AGCATGCTCC CCGTGAGCCA CAATGCCAGC 
CCACCTACCA GCTCCAGTCA GTACCCCAGC CTGTGGTCTG TGAGCAACGG CGCCGTCACC 
CCGGGCTCCC AGGCAGCAGC CGTGTCCAAC GGGCTGGGGG CCCAGTTCTT CCGGGGCTCC 
CCCGCGCACT ACACACCCCT CACCCATCCG GTCTCGGCGC CCTCTTCCTC GGGATCCCCA 
CTGTACGAAG GGGCGGCCGC GGCCACAGAC ATCGTGGACA GCCAGTACGA CGCCGCAGCC 
CAAGGCCGCC TCATAGCCTC ATGGACACCT GTGTCGCCAC CTTCCATGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TBXT ( NM_003181.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003172.1 Homo sapiens T-box transcription factor T (TBXT), transcript variant 1, mRNA.

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CloneID	OHu17741
Clone ID Related Accession (Same CDS sequence)	NM_003181.3
Accession Version	NM_003181.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1568476800000
Organism	Homo sapiens(human)
Product	T-box transcription factor T isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BE785653.1, BU190437.1, AJ001699.1, AL627443.6 and BC098425.1. This sequence is a reference standard in the RefSeqGene project. On Jul 11, 2012 this sequence version replaced NM_003181.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AJ001699.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC 
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT 
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT 
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC 
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC 
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC 
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG 
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC 
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC 
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA 
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG 
TACTCCCAAT GGGGGTGGCT TCTTCCTGGA ACCAGCACCC TGTGTCCACC TGCAAATCCT 
CATCCTCAGT TTGGAGGTGC CCTCTCCCTC CCCTCCACGC ACAGCTGTGA CAGGTACCCA 
ACCCTGAGGA GCCACCGGTC CTCACCCTAC CCCAGCCCCT ATGCTCATCG GAACAATTCT 
CCAACCTATT CTGACAACTC ACCTGCATGT TTATCCATGC TGCAATCCCA TGACAATTGG 
TCCAGCCTTG GAATGCCTGC CCATCCCAGC ATGCTCCCCG TGAGCCACAA TGCCAGCCCA 
CCTACCAGCT CCAGTCAGTA CCCCAGCCTG TGGTCTGTGA GCAACGGCGC CGTCACCCCG 
GGCTCCCAGG CAGCAGCCGT GTCCAACGGG CTGGGGGCCC AGTTCTTCCG GGGCTCCCCC 
GCGCACTACA CACCCCTCAC CCATCCGGTC TCGGCGCCCT CTTCCTCGGG ATCCCCACTG 
TACGAAGGGG CGGCCGCGGC CACAGACATC GTGGACAGCC AGTACGACGC CGCAGCCCAA 
GGCCGCCTCA TAGCCTCATG GACACCTGTG TCGCCACCTT CCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003172.1
CDS	520..1827
Translation	MSSPGTESAGKSLQYRVDHLLSAVENELQAGSEKGDPTERELRVGLEESELWLRFKELTNEMIVTKNGRRMFPVLKVNVSGLDPNAMYSFLLDFVAADNHRWKYVNGEWVPGGKPEPQAPSCVYIHPDSPNFGAHWMKAPVSFSKVKLTNKLNGGGQIMLNSLHKYEPRIHIVRVGGPQRMITSHCFPETQFIAVTAYQNEEITALKIKYNPFAKAFLDAKERSDHKEMMEEPGDSQQPGYSQWGWLLPGTSTLCPPANPHPQFGGALSLPSTHSCDRYPTLRSHRSSPYPSPYAHRNNSPTYSDNSPACLSMLQSHDNWSSLGMPAHPSMLPVSHNASPPTSSSQYPSLWSVSNGAVTPGSQAAAVSNGLGAQFFRGSPAHYTPLTHPVSAPSSSGSPLYEGAAAATDIVDSQYDAAAQGRLIASWTPVSPPSM

Target ORF information:

RefSeq Version	NM_003181.3
Organism	Homo sapiens(human)
Definition	Homo sapiens T-box transcription factor T (TBXT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003181.3

ORF Insert Sequence:

ATGAGCTCCC CTGGCACCGA GAGCGCGGGA AAGAGCCTGC AGTACCGAGT GGACCACCTG 
CTGAGCGCCG TGGAGAATGA GCTGCAGGCG GGCAGCGAGA AGGGCGACCC CACAGAGCGC 
GAACTGCGCG TGGGCCTGGA GGAGAGCGAG CTGTGGCTGC GCTTCAAGGA GCTCACCAAT 
GAGATGATCG TGACCAAGAA CGGCAGGAGG ATGTTTCCGG TGCTGAAGGT GAACGTGTCT 
GGCCTGGACC CCAACGCCAT GTACTCCTTC CTGCTGGACT TCGTGGCGGC GGACAACCAC 
CGCTGGAAGT ACGTGAACGG GGAATGGGTG CCGGGGGGCA AGCCGGAGCC GCAGGCGCCC 
AGCTGCGTCT ACATCCACCC CGACTCGCCC AACTTCGGGG CCCACTGGAT GAAGGCTCCC 
GTCTCCTTCA GCAAAGTCAA GCTCACCAAC AAGCTCAACG GAGGGGGCCA GATCATGCTG 
AACTCCTTGC ATAAGTATGA GCCTCGAATC CACATAGTGA GAGTTGGGGG TCCACAGCGC 
ATGATCACCA GCCACTGCTT CCCTGAGACC CAGTTCATAG CGGTGACTGC TTATCAGAAC 
GAGGAGATCA CAGCTCTTAA AATTAAGTAC AATCCATTTG CAAAAGCTTT CCTTGATGCA 
AAGGAAAGAA GTGATCACAA AGAGATGATG GAGGAACCCG GAGACAGCCA GCAACCTGGG 
TACTCCCAAT GGGGGTGGCT TCTTCCTGGA ACCAGCACCC TGTGTCCACC TGCAAATCCT 
CATCCTCAGT TTGGAGGTGC CCTCTCCCTC CCCTCCACGC ACAGCTGTGA CAGGTACCCA 
ACCCTGAGGA GCCACCGGTC CTCACCCTAC CCCAGCCCCT ATGCTCATCG GAACAATTCT 
CCAACCTATT CTGACAACTC ACCTGCATGT TTATCCATGC TGCAATCCCA TGACAATTGG 
TCCAGCCTTG GAATGCCTGC CCATCCCAGC ATGCTCCCCG TGAGCCACAA TGCCAGCCCA 
CCTACCAGCT CCAGTCAGTA CCCCAGCCTG TGGTCTGTGA GCAACGGCGC CGTCACCCCG 
GGCTCCCAGG CAGCAGCCGT GTCCAACGGG CTGGGGGCCC AGTTCTTCCG GGGCTCCCCC 
GCGCACTACA CACCCCTCAC CCATCCGGTC TCGGCGCCCT CTTCCTCGGG ATCCCCACTG 
TACGAAGGGG CGGCCGCGGC CACAGACATC GTGGACAGCC AGTACGACGC CGCAGCCCAA 
GGCCGCCTCA TAGCCTCATG GACACCTGTG TCGCCACCTT CCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transfection of T-Box Transcription Factor
International journal of molecular sciences19(11)(2018 Nov)
Akimoto N,Nakamura K,Hijioka H,Kume K,Matsumura Y,Sugiura T

Brachyury oncogene is a prognostic factor in high-risk testicular germ cell tumors.
Andrology6(4)597-604(2018 07)
Pinto F,Cárcano FM,da Silva ECA,Vidal DO,Scapulatempo-Neto C,Lopes LF,Reis RM

Brachyury gene copy number gain and activation of the PI3K/Akt pathway: association with upregulation of oncogenic Brachyury expression in skull base chordoma.
Journal of neurosurgery128(5)1428-1437(2018 05)
Otani R,Mukasa A,Shin M,Omata M,Takayanagi S,Tanaka S,Ueki K,Saito N

The lipodystrophic hotspot lamin A p.R482W mutation deregulates the mesodermal inducer T/Brachyury and early vascular differentiation gene networks.
Human molecular genetics27(8)1447-1459(2018 04)
Briand N,Guénantin AC,Jeziorowska D,Shah A,Mantecon M,Capel E,Garcia M,Oldenburg A,Paulsen J,Hulot JS,Vigouroux C,Collas P

The driver landscape of sporadic chordoma.
Nature communications8(1)890(2017 10)
Tarpey PS,Behjati S,Young MD,Martincorena I,Alexandrov LB,Farndon SJ,Guzzo C,Hardy C,Latimer C,Butler AP,Teague JW,Shlien A,Futreal PA,Shah S,Bashashati A,Jamshidi F,Nielsen TO,Huntsman D,Baumhoer D,Brandner S,Wunder J,Dickson B,Cogswell P,Sommer J,Phillips JJ,Amary MF,Tirabosco R,Pillay N,Yip S,Stratton MR,Flanagan AM,Campbell PJ

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TBXT cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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