KCNK10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KCNK10
Entrez Gene ID	54207
Full Name	potassium two pore domain channel subfamily K member 10
Synonyms	K2p10.1,PPP1R97,TREK-2,TREK2
General protein information	Preferred Names potassium two pore domain channel subfamily K member 10 Names potassium channel subfamily K member 10 2P domain potassium channel TREK2 TREK-2 K(+) channel subunit TWIK-related K+ channel 2 outward rectifying potassium channel protein TREK-2 potassium channel TREK-2 potassium channel, subfamily K, member 10 potassium channel, two pore domain subfamily K, member 10 protein phosphatase 1, regulatory subunit 97
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q31.3
Summary	The protein encoded by this gene belongs to the family of potassium channel proteins containing two pore-forming P domains. This channel is an open rectifier which primarily passes outward current under physiological K+ concentrations, and is stimulated strongly by arachidonic acid and to a lesser degree by membrane stretching, intracellular acidification, and general anaesthetics. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Sep 2008].
Disorder MIM:	605873

mRNA and Protein(s)

mRNA	Protein	Name
XM_011536840.2	XP_011535142.1	potassium channel subfamily K member 10 isoform X2
NM_138317.3	NP_612190.1	potassium channel subfamily K member 10 isoform 2
NM_138318.2	NP_612191.1	potassium channel subfamily K member 10 isoform 3
NM_021161.4	NP_066984.1	potassium channel subfamily K member 10 isoform 1 precursor
NM_138317.2	NP_612190.1	potassium channel subfamily K member 10 isoform 2
XM_024449628.1	XP_024305396.1	potassium channel subfamily K member 10 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	KCNK10	XP_001136938.1
Canis lupus familiaris (dog)	KCNK10	XP_005623781.1
Rattus norvegicus (Norway rat)	Kcnk10	NP_075584.1
Danio rerio (zebrafish)	LOC563228	XP_691684.4
Homo sapiens (human)	KCNK10	NP_612190.1
Gallus gallus (chicken)	KCNK10	XP_004941911.1
Macaca mulatta (Rhesus monkey)	KCNK10	XP_002805229.1
Mus musculus (house mouse)	Kcnk10	NP_084187.2
Xenopus tropicalis (tropical clawed frog)	kcnk10	XP_002933240.1
Bos taurus (cattle)	KCNK10	XP_002691132.2
Danio rerio (zebrafish)	kcnk10b	NP_001154871.1

Gene Ontology
Bibliography

Related articles in PubMed

The glycine hinge of transmembrane segment 2 modulates the subcellular localization and gating properties in TREK channels.
Zhuo RG, Peng P, Zheng JQ, Zhang YL, Wen L, Wei XL, Ma XY
Biochemical and biophysical research communications490(3)1125-1131(2017 08)

Polymodal activation of the TREK-2 K2P channel produces structurally distinct open states.
McClenaghan C, Schewe M, Aryal P, Carpenter EP, Baukrowitz T, Tucker SJ
The Journal of general physiology147(6)497-505(2016 06)

The isoforms generated by alternative translation initiation adopt similar conformation in the selectivity filter in TREK-2.
Zhuo RG, Peng P, Liu XY, Zhang SZ, Xu JP, Zheng JQ, Wei XL, Ma XY
Journal of physiology and biochemistry71(4)601-10(2015 Dec)

The association of DNA methylation and brain volume in healthy individuals and schizophrenia patients.
Liu J, Siyahhan Julnes P, Chen J, Ehrlich S, Walton E, Calhoun VD
Schizophrenia research169(1-3)447-452(2015 Dec)

How ion channels sense mechanical force: insights from mechanosensitive K2P channels TRAAK, TREK1, and TREK2.
Brohawn SG
Annals of the New York Academy of Sciences135220-32(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following KCNK10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KCNK10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu103923	XM_024449628.1 Latest version!	Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu68283	XM_011536840.2 Latest version!	Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu28013	NM_138317.3 Latest version!	Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu28007	NM_021161.4 Latest version!	Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$321.30 $459.00
OHu28024	NM_138318.2 Latest version!	Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu28013	NM_138317.2	Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KCNK10 ( XM_024449628.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305396.1 Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant X1, mRNA.

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CloneID	OHu103923
Clone ID Related Accession (Same CDS sequence)	XM_024449628.1
Accession Version	XM_024449628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	potassium channel subfamily K member 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGCA CCTCCCAAGG GGGCTTGCAG ACCGTCATGA AGTGGAAGAC GGTGGTTGCC 
ATCTTTGTGG TTGTGGTGGT CTACCTTGTC ACTGGCGGTC TTGTCTTCCG GGCATTGGAG 
CAGCCCTTTG AGAGCAGCCA GAAGAATACC ATCGCCTTGG AGAAGGCGGA ATTCCTGCGG 
GATCATGTCT GTGTGAGCCC CCAGGAGCTG GAGACGTTGA TCCAGCATGC TCTTGATGCT 
GACAATGCGG GAGTCAGTCC AATAGGAAAC TCTTCCAACA ACAGCAGCCA CTGGGACCTC 
GGCAGTGCCT TTTTCTTTGC TGGAACTGTC ATTACGACCA TAGGGTATGG GAATATTGCT 
CCGAGCACTG AAGGAGGCAA AATCTTTTGT ATTTTATATG CCATCTTTGG AATTCCACTC 
TTTGGTTTCT TATTGGCTGG AATTGGAGAC CAACTTGGAA CCATCTTTGG GAAAAGCATT 
GCAAGAGTGG AGAAGGTCTT TCGAAAAAAG CAAGTGAGTC AGACCAAGAT CCGGGTCATC 
TCAACCATCC TGTTCATCTT GGCCGGCTGC ATTGTGTTTG TGACGATCCC TGCTGTCATC 
TTTAAGTACA TCGAGGGCTG GACGGCCTTG GAGTCCATTT ACTTTGTGGT GGTCACTCTG 
ACCACGGTGG GCTTTGGTGA TTTTGTGGCA GGGGGAAACG CTGGCATCAA TTATCGGGAG 
TGGTATAAGC CCCTAGTGTG GTTTTGGATC CTTGTTGGCC TTGCCTACTT TGCAGCTGTC 
CTCAGTATGA TCGGAGATTG GCTACGGGTT CTGTCCAAAA AGACAAAAGA AGAGGTGGGT 
GAAATCAAGG CCCATGCGGC AGAGTGGAAG GCCAATGTCA CGGCTGAGTT CCGGGAGACA 
CGGCGAAGGC TCAGCGTGGA GATCCACGAT AAGCTGCAGC GGGCGGCCAC CATCCGCAGC 
ATGGAGCGCC GGCGGCTGGG CCTGGACCAG CGGGCCCACT CACTGGACAT GCTGTCCCCC 
GAGAAGCGCT CTGTCTTTGC TGCCCTGGAC ACCGGCCGCT TCAAGGCCTC ATCCCAGGAG 
AGCATCAACA ACCGGCCCAA CAACCTGCGC CTGAAGGGGC CGGAGCAGCT GAACAAGCAT 
GGGCAGGGTG CGTCCGAGGA CAACATCATC AACAAGTTCG GGTCCACCTC CAGACTCACC 
AAGAGGAAAA ACAAGGACCT CAAAAAGACC TTGCCCGAGG ACGTTCAGAA AATCTACAAG 
ACCTTCCGGA ATTACTCCCT GGACGAGGAG AAGAAAGAGG AGGAGACGGA AAAGATGTGT 
AACTCAGACA ACTCCAGCAC AGCCATGCTG ACGGACTGTA TCCAGCAGCA CGCTGAGTTG 
GAGAACGGAA TGATACCCAC GGACACCAAA GACCGGGAGC CGGAGAACAA CTCATTACTT 
GAAGACAGAA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305396.1
CDS	1521..2975
Translation	MEGTSQGGLQTVMKWKTVVAIFVVVVVYLVTGGLVFRALEQPFESSQKNTIALEKAEFLRDHVCVSPQELETLIQHALDADNAGVSPIGNSSNNSSHWDLGSAFFFAGTVITTIGYGNIAPSTEGGKIFCILYAIFGIPLFGFLLAGIGDQLGTIFGKSIARVEKVFRKKQVSQTKIRVISTILFILAGCIVFVTIPAVIFKYIEGWTALESIYFVVVTLTTVGFGDFVAGGNAGINYREWYKPLVWFWILVGLAYFAAVLSMIGDWLRVLSKKTKEEVGEIKAHAAEWKANVTAEFRETRRRLSVEIHDKLQRAATIRSMERRRLGLDQRAHSLDMLSPEKRSVFAALDTGRFKASSQESINNRPNNLRLKGPEQLNKHGQGASEDNIINKFGSTSRLTKRKNKDLKKTLPEDVQKIYKTFRNYSLDEEKKEEETEKMCNSDNSSTAMLTDCIQQHAELENGMIPTDTKDREPENNSLLEDRN

Target ORF information:

RefSeq Version	XM_024449628.1
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449628.1

ORF Insert Sequence:

ATGGAAGGCA CCTCCCAAGG GGGCTTGCAG ACCGTCATGA AGTGGAAGAC GGTGGTTGCC 
ATCTTTGTGG TTGTGGTGGT CTACCTTGTC ACTGGCGGTC TTGTCTTCCG GGCATTGGAG 
CAGCCCTTTG AGAGCAGCCA GAAGAATACC ATCGCCTTGG AGAAGGCGGA ATTCCTGCGG 
GATCATGTCT GTGTGAGCCC CCAGGAGCTG GAGACGTTGA TCCAGCATGC TCTTGATGCT 
GACAATGCGG GAGTCAGTCC AATAGGAAAC TCTTCCAACA ACAGCAGCCA CTGGGACCTC 
GGCAGTGCCT TTTTCTTTGC TGGAACTGTC ATTACGACCA TAGGGTATGG GAATATTGCT 
CCGAGCACTG AAGGAGGCAA AATCTTTTGT ATTTTATATG CCATCTTTGG AATTCCACTC 
TTTGGTTTCT TATTGGCTGG AATTGGAGAC CAACTTGGAA CCATCTTTGG GAAAAGCATT 
GCAAGAGTGG AGAAGGTCTT TCGAAAAAAG CAAGTGAGTC AGACCAAGAT CCGGGTCATC 
TCAACCATCC TGTTCATCTT GGCCGGCTGC ATTGTGTTTG TGACGATCCC TGCTGTCATC 
TTTAAGTACA TCGAGGGCTG GACGGCCTTG GAGTCCATTT ACTTTGTGGT GGTCACTCTG 
ACCACGGTGG GCTTTGGTGA TTTTGTGGCA GGGGGAAACG CTGGCATCAA TTATCGGGAG 
TGGTATAAGC CCCTAGTGTG GTTTTGGATC CTTGTTGGCC TTGCCTACTT TGCAGCTGTC 
CTCAGTATGA TCGGAGATTG GCTACGGGTT CTGTCCAAAA AGACAAAAGA AGAGGTGGGT 
GAAATCAAGG CCCATGCGGC AGAGTGGAAG GCCAATGTCA CGGCTGAGTT CCGGGAGACA 
CGGCGAAGGC TCAGCGTGGA GATCCACGAT AAGCTGCAGC GGGCGGCCAC CATCCGCAGC 
ATGGAGCGCC GGCGGCTGGG CCTGGACCAG CGGGCCCACT CACTGGACAT GCTGTCCCCC 
GAGAAGCGCT CTGTCTTTGC TGCCCTGGAC ACCGGCCGCT TCAAGGCCTC ATCCCAGGAG 
AGCATCAACA ACCGGCCCAA CAACCTGCGC CTGAAGGGGC CGGAGCAGCT GAACAAGCAT 
GGGCAGGGTG CGTCCGAGGA CAACATCATC AACAAGTTCG GGTCCACCTC CAGACTCACC 
AAGAGGAAAA ACAAGGACCT CAAAAAGACC TTGCCCGAGG ACGTTCAGAA AATCTACAAG 
ACCTTCCGGA ATTACTCCCT GGACGAGGAG AAGAAAGAGG AGGAGACGGA AAAGATGTGT 
AACTCAGACA ACTCCAGCAC AGCCATGCTG ACGGACTGTA TCCAGCAGCA CGCTGAGTTG 
GAGAACGGAA TGATACCCAC GGACACCAAA GACCGGGAGC CGGAGAACAA CTCATTACTT 
GAAGACAGAA ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNK10 ( XM_011536840.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535142.1 Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu68283
Clone ID Related Accession (Same CDS sequence)	XM_011536840.2
Accession Version	XM_011536840.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1575475200000
Organism	Homo sapiens(human)
Product	potassium channel subfamily K member 10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536840.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATGCTC TTGATGCTGA CAATGCGGGA GTCAGTCCAA TAGGAAACTC TTCCAACAAC 
AGCAGCCACT GGGACCTCGG CAGTGCCTTT TTCTTTGCTG GAACTGTCAT TACGACCATA 
GGGTATGGGA ATATTGCTCC GAGCACTGAA GGAGGCAAAA TCTTTTGTAT TTTATATGCC 
ATCTTTGGAA TTCCACTCTT TGGTTTCTTA TTGGCTGGAA TTGGAGACCA ACTTGGAACC 
ATCTTTGGGA AAAGCATTGC AAGAGTGGAG AAGGTCTTTC GAAAAAAGCA AGTGAGTCAG 
ACCAAGATCC GGGTCATCTC AACCATCCTG TTCATCTTGG CCGGCTGCAT TGTGTTTGTG 
ACGATCCCTG CTGTCATCTT TAAGTACATC GAGGGCTGGA CGGCCTTGGA GTCCATTTAC 
TTTGTGGTGG TCACTCTGAC CACGGTGGGC TTTGGTGATT TTGTGGCAGG GGGAAACGCT 
GGCATCAATT ATCGGGAGTG GTATAAGCCC CTAGTGTGGT TTTGGATCCT TGTTGGCCTT 
GCCTACTTTG CAGCTGTCCT CAGTATGATC GGAGATTGGC TACGGGTTCT GTCCAAAAAG 
ACAAAAGAAG AGGTGGGTGA AATCAAGGCC CATGCGGCAG AGTGGAAGGC CAATGTCACG 
GCTGAGTTCC GGGAGACACG GCGAAGGCTC AGCGTGGAGA TCCACGATAA GCTGCAGCGG 
GCGGCCACCA TCCGCAGCAT GGAGCGCCGG CGGCTGGGCC TGGACCAGCG GGCCCACTCA 
CTGGACATGC TGTCCCCCGA GAAGCGCTCT GTCTTTGCTG CCCTGGACAC CGGCCGCTTC 
AAGGCCTCAT CCCAGGAGAG CATCAACAAC CGGCCCAACA ACCTGCGCCT GAAGGGGCCG 
GAGCAGCTGA ACAAGCATGG GCAGGGTGCG TCCGAGGACA ACATCATCAA CAAGTTCGGG 
TCCACCTCCA GACTCACCAA GAGGAAAAAC AAGGACCTCA AAAAGACCTT GCCCGAGGAC 
GTTCAGAAAA TCTACAAGAC CTTCCGGAAT TACTCCCTGG ACGAGGAGAA GAAAGAGGAG 
GAGACGGAAA AGATGTGTAA CTCAGACAAC TCCAGCACAG CCATGCTGAC GGACTGTATC 
CAGCAGCACG CTGAGTTGGA GAACGGAATG ATACCCACGG ACACCAAAGA CCGGGAGCCG 
GAGAACAACT CATTACTTGA AGACAGAAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535142.1
CDS	227..1459
Translation	MHALDADNAGVSPIGNSSNNSSHWDLGSAFFFAGTVITTIGYGNIAPSTEGGKIFCILYAIFGIPLFGFLLAGIGDQLGTIFGKSIARVEKVFRKKQVSQTKIRVISTILFILAGCIVFVTIPAVIFKYIEGWTALESIYFVVVTLTTVGFGDFVAGGNAGINYREWYKPLVWFWILVGLAYFAAVLSMIGDWLRVLSKKTKEEVGEIKAHAAEWKANVTAEFRETRRRLSVEIHDKLQRAATIRSMERRRLGLDQRAHSLDMLSPEKRSVFAALDTGRFKASSQESINNRPNNLRLKGPEQLNKHGQGASEDNIINKFGSTSRLTKRKNKDLKKTLPEDVQKIYKTFRNYSLDEEKKEEETEKMCNSDNSSTAMLTDCIQQHAELENGMIPTDTKDREPENNSLLEDRN

Target ORF information:

RefSeq Version	XM_011536840.2
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536840.2

ORF Insert Sequence:

ATGCATGCTC TTGATGCTGA CAATGCGGGA GTCAGTCCAA TAGGAAACTC TTCCAACAAC 
AGCAGCCACT GGGACCTCGG CAGTGCCTTT TTCTTTGCTG GAACTGTCAT TACGACCATA 
GGGTATGGGA ATATTGCTCC GAGCACTGAA GGAGGCAAAA TCTTTTGTAT TTTATATGCC 
ATCTTTGGAA TTCCACTCTT TGGTTTCTTA TTGGCTGGAA TTGGAGACCA ACTTGGAACC 
ATCTTTGGGA AAAGCATTGC AAGAGTGGAG AAGGTCTTTC GAAAAAAGCA AGTGAGTCAG 
ACCAAGATCC GGGTCATCTC AACCATCCTG TTCATCTTGG CCGGCTGCAT TGTGTTTGTG 
ACGATCCCTG CTGTCATCTT TAAGTACATC GAGGGCTGGA CGGCCTTGGA GTCCATTTAC 
TTTGTGGTGG TCACTCTGAC CACGGTGGGC TTTGGTGATT TTGTGGCAGG GGGAAACGCT 
GGCATCAATT ATCGGGAGTG GTATAAGCCC CTAGTGTGGT TTTGGATCCT TGTTGGCCTT 
GCCTACTTTG CAGCTGTCCT CAGTATGATC GGAGATTGGC TACGGGTTCT GTCCAAAAAG 
ACAAAAGAAG AGGTGGGTGA AATCAAGGCC CATGCGGCAG AGTGGAAGGC CAATGTCACG 
GCTGAGTTCC GGGAGACACG GCGAAGGCTC AGCGTGGAGA TCCACGATAA GCTGCAGCGG 
GCGGCCACCA TCCGCAGCAT GGAGCGCCGG CGGCTGGGCC TGGACCAGCG GGCCCACTCA 
CTGGACATGC TGTCCCCCGA GAAGCGCTCT GTCTTTGCTG CCCTGGACAC CGGCCGCTTC 
AAGGCCTCAT CCCAGGAGAG CATCAACAAC CGGCCCAACA ACCTGCGCCT GAAGGGGCCG 
GAGCAGCTGA ACAAGCATGG GCAGGGTGCG TCCGAGGACA ACATCATCAA CAAGTTCGGG 
TCCACCTCCA GACTCACCAA GAGGAAAAAC AAGGACCTCA AAAAGACCTT GCCCGAGGAC 
GTTCAGAAAA TCTACAAGAC CTTCCGGAAT TACTCCCTGG ACGAGGAGAA GAAAGAGGAG 
GAGACGGAAA AGATGTGTAA CTCAGACAAC TCCAGCACAG CCATGCTGAC GGACTGTATC 
CAGCAGCACG CTGAGTTGGA GAACGGAATG ATACCCACGG ACACCAAAGA CCGGGAGCCG 
GAGAACAACT CATTACTTGA AGACAGAAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNK10 ( NM_138317.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_612190.1 Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28013
Clone ID Related Accession (Same CDS sequence)	NM_138317.3 , NM_138317.2
Accession Version	NM_138317.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559318400000
Organism	Homo sapiens(human)
Product	potassium channel subfamily K member 10 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL049834.3, AK313499.1, EU978939.1, AF279890.1, AL133279.7, AI693366.1 and U79244.1. On Jun 2, 2019 this sequence version replaced NM_138317.2. Transcript Variant: This variant (2, also known as TREK2 splice variant c) has a different 5' terminal exon compared to transcript variant 1, and encodes a slightly longer isoform (2) with a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.33356.1, SRR1803614.141814.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAATTTC CAATCGAGAC GCCAAGAAAA CAGGTGAACT GGGATCCTAA AGTGGCCGTT 
CCCGCAGCAG CACCGGTGTG CCAGCCCAAG AGCGCCACTA ACGGGCAACC CCCGGCTCCG 
GCTCCGACTC CAACTCCGCG CCTGTCCATT TCCTCCCGAG CCACAGTGGT AGCCAGGATG 
GAAGGCACCT CCCAAGGGGG CTTGCAGACC GTCATGAAGT GGAAGACGGT GGTTGCCATC 
TTTGTGGTTG TGGTGGTCTA CCTTGTCACT GGCGGTCTTG TCTTCCGGGC ATTGGAGCAG 
CCCTTTGAGA GCAGCCAGAA GAATACCATC GCCTTGGAGA AGGCGGAATT CCTGCGGGAT 
CATGTCTGTG TGAGCCCCCA GGAGCTGGAG ACGTTGATCC AGCATGCTCT TGATGCTGAC 
AATGCGGGAG TCAGTCCAAT AGGAAACTCT TCCAACAACA GCAGCCACTG GGACCTCGGC 
AGTGCCTTTT TCTTTGCTGG AACTGTCATT ACGACCATAG GGTATGGGAA TATTGCTCCG 
AGCACTGAAG GAGGCAAAAT CTTTTGTATT TTATATGCCA TCTTTGGAAT TCCACTCTTT 
GGTTTCTTAT TGGCTGGAAT TGGAGACCAA CTTGGAACCA TCTTTGGGAA AAGCATTGCA 
AGAGTGGAGA AGGTCTTTCG AAAAAAGCAA GTGAGTCAGA CCAAGATCCG GGTCATCTCA 
ACCATCCTGT TCATCTTGGC CGGCTGCATT GTGTTTGTGA CGATCCCTGC TGTCATCTTT 
AAGTACATCG AGGGCTGGAC GGCCTTGGAG TCCATTTACT TTGTGGTGGT CACTCTGACC 
ACGGTGGGCT TTGGTGATTT TGTGGCAGGG GGAAACGCTG GCATCAATTA TCGGGAGTGG 
TATAAGCCCC TAGTGTGGTT TTGGATCCTT GTTGGCCTTG CCTACTTTGC AGCTGTCCTC 
AGTATGATCG GAGATTGGCT ACGGGTTCTG TCCAAAAAGA CAAAAGAAGA GGTGGGTGAA 
ATCAAGGCCC ATGCGGCAGA GTGGAAGGCC AATGTCACGG CTGAGTTCCG GGAGACACGG 
CGAAGGCTCA GCGTGGAGAT CCACGATAAG CTGCAGCGGG CGGCCACCAT CCGCAGCATG 
GAGCGCCGGC GGCTGGGCCT GGACCAGCGG GCCCACTCAC TGGACATGCT GTCCCCCGAG 
AAGCGCTCTG TCTTTGCTGC CCTGGACACC GGCCGCTTCA AGGCCTCATC CCAGGAGAGC 
ATCAACAACC GGCCCAACAA CCTGCGCCTG AAGGGGCCGG AGCAGCTGAA CAAGCATGGG 
CAGGGTGCGT CCGAGGACAA CATCATCAAC AAGTTCGGGT CCACCTCCAG ACTCACCAAG 
AGGAAAAACA AGGACCTCAA AAAGACCTTG CCCGAGGACG TTCAGAAAAT CTACAAGACC 
TTCCGGAATT ACTCCCTGGA CGAGGAGAAG AAAGAGGAGG AGACGGAAAA GATGTGTAAC 
TCAGACAACT CCAGCACAGC CATGCTGACG GACTGTATCC AGCAGCACGC TGAGTTGGAG 
AACGGAATGA TACCCACGGA CACCAAAGAC CGGGAGCCGG AGAACAACTC ATTACTTGAA 
GACAGAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612190.1
CDS	472..2103
Translation	MKFPIETPRKQVNWDPKVAVPAAAPVCQPKSATNGQPPAPAPTPTPRLSISSRATVVARMEGTSQGGLQTVMKWKTVVAIFVVVVVYLVTGGLVFRALEQPFESSQKNTIALEKAEFLRDHVCVSPQELETLIQHALDADNAGVSPIGNSSNNSSHWDLGSAFFFAGTVITTIGYGNIAPSTEGGKIFCILYAIFGIPLFGFLLAGIGDQLGTIFGKSIARVEKVFRKKQVSQTKIRVISTILFILAGCIVFVTIPAVIFKYIEGWTALESIYFVVVTLTTVGFGDFVAGGNAGINYREWYKPLVWFWILVGLAYFAAVLSMIGDWLRVLSKKTKEEVGEIKAHAAEWKANVTAEFRETRRRLSVEIHDKLQRAATIRSMERRRLGLDQRAHSLDMLSPEKRSVFAALDTGRFKASSQESINNRPNNLRLKGPEQLNKHGQGASEDNIINKFGSTSRLTKRKNKDLKKTLPEDVQKIYKTFRNYSLDEEKKEEETEKMCNSDNSSTAMLTDCIQQHAELENGMIPTDTKDREPENNSLLEDRN

Target ORF information:

RefSeq Version	NM_138317.3
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138317.3

ORF Insert Sequence:

ATGAAATTTC CAATCGAGAC GCCAAGAAAA CAGGTGAACT GGGATCCTAA AGTGGCCGTT 
CCCGCAGCAG CACCGGTGTG CCAGCCCAAG AGCGCCACTA ACGGGCAACC CCCGGCTCCG 
GCTCCGACTC CAACTCCGCG CCTGTCCATT TCCTCCCGAG CCACAGTGGT AGCCAGGATG 
GAAGGCACCT CCCAAGGGGG CTTGCAGACC GTCATGAAGT GGAAGACGGT GGTTGCCATC 
TTTGTGGTTG TGGTGGTCTA CCTTGTCACT GGCGGTCTTG TCTTCCGGGC ATTGGAGCAG 
CCCTTTGAGA GCAGCCAGAA GAATACCATC GCCTTGGAGA AGGCGGAATT CCTGCGGGAT 
CATGTCTGTG TGAGCCCCCA GGAGCTGGAG ACGTTGATCC AGCATGCTCT TGATGCTGAC 
AATGCGGGAG TCAGTCCAAT AGGAAACTCT TCCAACAACA GCAGCCACTG GGACCTCGGC 
AGTGCCTTTT TCTTTGCTGG AACTGTCATT ACGACCATAG GGTATGGGAA TATTGCTCCG 
AGCACTGAAG GAGGCAAAAT CTTTTGTATT TTATATGCCA TCTTTGGAAT TCCACTCTTT 
GGTTTCTTAT TGGCTGGAAT TGGAGACCAA CTTGGAACCA TCTTTGGGAA AAGCATTGCA 
AGAGTGGAGA AGGTCTTTCG AAAAAAGCAA GTGAGTCAGA CCAAGATCCG GGTCATCTCA 
ACCATCCTGT TCATCTTGGC CGGCTGCATT GTGTTTGTGA CGATCCCTGC TGTCATCTTT 
AAGTACATCG AGGGCTGGAC GGCCTTGGAG TCCATTTACT TTGTGGTGGT CACTCTGACC 
ACGGTGGGCT TTGGTGATTT TGTGGCAGGG GGAAACGCTG GCATCAATTA TCGGGAGTGG 
TATAAGCCCC TAGTGTGGTT TTGGATCCTT GTTGGCCTTG CCTACTTTGC AGCTGTCCTC 
AGTATGATCG GAGATTGGCT ACGGGTTCTG TCCAAAAAGA CAAAAGAAGA GGTGGGTGAA 
ATCAAGGCCC ATGCGGCAGA GTGGAAGGCC AATGTCACGG CTGAGTTCCG GGAGACACGG 
CGAAGGCTCA GCGTGGAGAT CCACGATAAG CTGCAGCGGG CGGCCACCAT CCGCAGCATG 
GAGCGCCGGC GGCTGGGCCT GGACCAGCGG GCCCACTCAC TGGACATGCT GTCCCCCGAG 
AAGCGCTCTG TCTTTGCTGC CCTGGACACC GGCCGCTTCA AGGCCTCATC CCAGGAGAGC 
ATCAACAACC GGCCCAACAA CCTGCGCCTG AAGGGGCCGG AGCAGCTGAA CAAGCATGGG 
CAGGGTGCGT CCGAGGACAA CATCATCAAC AAGTTCGGGT CCACCTCCAG ACTCACCAAG 
AGGAAAAACA AGGACCTCAA AAAGACCTTG CCCGAGGACG TTCAGAAAAT CTACAAGACC 
TTCCGGAATT ACTCCCTGGA CGAGGAGAAG AAAGAGGAGG AGACGGAAAA GATGTGTAAC 
TCAGACAACT CCAGCACAGC CATGCTGACG GACTGTATCC AGCAGCACGC TGAGTTGGAG 
AACGGAATGA TACCCACGGA CACCAAAGAC CGGGAGCCGG AGAACAACTC ATTACTTGAA 
GACAGAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNK10 ( NM_138317.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_612190.1 Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant 2, mRNA.

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CloneID	OHu28013
Clone ID Related Accession (Same CDS sequence)	NM_138317.3 , NM_138317.2
Accession Version	NM_138317.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1529683200000
Organism	Homo sapiens(human)
Product	potassium channel subfamily K member 10 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK313499.1, EU978939.1, AF279890.1, AL133279.7, AI693366.1 and U79244.1. On Sep 20, 2008 this sequence version replaced NM_138317.1. Transcript Variant: This variant (2, also known as TREK2 splice variant c) has a different 5' terminal exon compared to transcript variant 1, and encodes a slightly longer isoform (2) with a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK313499.1, SRR1803614.141814.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAATTTC CAATCGAGAC GCCAAGAAAA CAGGTGAACT GGGATCCTAA AGTGGCCGTT 
CCCGCAGCAG CACCGGTGTG CCAGCCCAAG AGCGCCACTA ACGGGCAACC CCCGGCTCCG 
GCTCCGACTC CAACTCCGCG CCTGTCCATT TCCTCCCGAG CCACAGTGGT AGCCAGGATG 
GAAGGCACCT CCCAAGGGGG CTTGCAGACC GTCATGAAGT GGAAGACGGT GGTTGCCATC 
TTTGTGGTTG TGGTGGTCTA CCTTGTCACT GGCGGTCTTG TCTTCCGGGC ATTGGAGCAG 
CCCTTTGAGA GCAGCCAGAA GAATACCATC GCCTTGGAGA AGGCGGAATT CCTGCGGGAT 
CATGTCTGTG TGAGCCCCCA GGAGCTGGAG ACGTTGATCC AGCATGCTCT TGATGCTGAC 
AATGCGGGAG TCAGTCCAAT AGGAAACTCT TCCAACAACA GCAGCCACTG GGACCTCGGC 
AGTGCCTTTT TCTTTGCTGG AACTGTCATT ACGACCATAG GGTATGGGAA TATTGCTCCG 
AGCACTGAAG GAGGCAAAAT CTTTTGTATT TTATATGCCA TCTTTGGAAT TCCACTCTTT 
GGTTTCTTAT TGGCTGGAAT TGGAGACCAA CTTGGAACCA TCTTTGGGAA AAGCATTGCA 
AGAGTGGAGA AGGTCTTTCG AAAAAAGCAA GTGAGTCAGA CCAAGATCCG GGTCATCTCA 
ACCATCCTGT TCATCTTGGC CGGCTGCATT GTGTTTGTGA CGATCCCTGC TGTCATCTTT 
AAGTACATCG AGGGCTGGAC GGCCTTGGAG TCCATTTACT TTGTGGTGGT CACTCTGACC 
ACGGTGGGCT TTGGTGATTT TGTGGCAGGG GGAAACGCTG GCATCAATTA TCGGGAGTGG 
TATAAGCCCC TAGTGTGGTT TTGGATCCTT GTTGGCCTTG CCTACTTTGC AGCTGTCCTC 
AGTATGATCG GAGATTGGCT ACGGGTTCTG TCCAAAAAGA CAAAAGAAGA GGTGGGTGAA 
ATCAAGGCCC ATGCGGCAGA GTGGAAGGCC AATGTCACGG CTGAGTTCCG GGAGACACGG 
CGAAGGCTCA GCGTGGAGAT CCACGATAAG CTGCAGCGGG CGGCCACCAT CCGCAGCATG 
GAGCGCCGGC GGCTGGGCCT GGACCAGCGG GCCCACTCAC TGGACATGCT GTCCCCCGAG 
AAGCGCTCTG TCTTTGCTGC CCTGGACACC GGCCGCTTCA AGGCCTCATC CCAGGAGAGC 
ATCAACAACC GGCCCAACAA CCTGCGCCTG AAGGGGCCGG AGCAGCTGAA CAAGCATGGG 
CAGGGTGCGT CCGAGGACAA CATCATCAAC AAGTTCGGGT CCACCTCCAG ACTCACCAAG 
AGGAAAAACA AGGACCTCAA AAAGACCTTG CCCGAGGACG TTCAGAAAAT CTACAAGACC 
TTCCGGAATT ACTCCCTGGA CGAGGAGAAG AAAGAGGAGG AGACGGAAAA GATGTGTAAC 
TCAGACAACT CCAGCACAGC CATGCTGACG GACTGTATCC AGCAGCACGC TGAGTTGGAG 
AACGGAATGA TACCCACGGA CACCAAAGAC CGGGAGCCGG AGAACAACTC ATTACTTGAA 
GACAGAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612190.1
CDS	452..2083
Translation	MKFPIETPRKQVNWDPKVAVPAAAPVCQPKSATNGQPPAPAPTPTPRLSISSRATVVARMEGTSQGGLQTVMKWKTVVAIFVVVVVYLVTGGLVFRALEQPFESSQKNTIALEKAEFLRDHVCVSPQELETLIQHALDADNAGVSPIGNSSNNSSHWDLGSAFFFAGTVITTIGYGNIAPSTEGGKIFCILYAIFGIPLFGFLLAGIGDQLGTIFGKSIARVEKVFRKKQVSQTKIRVISTILFILAGCIVFVTIPAVIFKYIEGWTALESIYFVVVTLTTVGFGDFVAGGNAGINYREWYKPLVWFWILVGLAYFAAVLSMIGDWLRVLSKKTKEEVGEIKAHAAEWKANVTAEFRETRRRLSVEIHDKLQRAATIRSMERRRLGLDQRAHSLDMLSPEKRSVFAALDTGRFKASSQESINNRPNNLRLKGPEQLNKHGQGASEDNIINKFGSTSRLTKRKNKDLKKTLPEDVQKIYKTFRNYSLDEEKKEEETEKMCNSDNSSTAMLTDCIQQHAELENGMIPTDTKDREPENNSLLEDRN

Target ORF information:

RefSeq Version	NM_138317.2
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138317.2

ORF Insert Sequence:

ATGAAATTTC CAATCGAGAC GCCAAGAAAA CAGGTGAACT GGGATCCTAA AGTGGCCGTT 
CCCGCAGCAG CACCGGTGTG CCAGCCCAAG AGCGCCACTA ACGGGCAACC CCCGGCTCCG 
GCTCCGACTC CAACTCCGCG CCTGTCCATT TCCTCCCGAG CCACAGTGGT AGCCAGGATG 
GAAGGCACCT CCCAAGGGGG CTTGCAGACC GTCATGAAGT GGAAGACGGT GGTTGCCATC 
TTTGTGGTTG TGGTGGTCTA CCTTGTCACT GGCGGTCTTG TCTTCCGGGC ATTGGAGCAG 
CCCTTTGAGA GCAGCCAGAA GAATACCATC GCCTTGGAGA AGGCGGAATT CCTGCGGGAT 
CATGTCTGTG TGAGCCCCCA GGAGCTGGAG ACGTTGATCC AGCATGCTCT TGATGCTGAC 
AATGCGGGAG TCAGTCCAAT AGGAAACTCT TCCAACAACA GCAGCCACTG GGACCTCGGC 
AGTGCCTTTT TCTTTGCTGG AACTGTCATT ACGACCATAG GGTATGGGAA TATTGCTCCG 
AGCACTGAAG GAGGCAAAAT CTTTTGTATT TTATATGCCA TCTTTGGAAT TCCACTCTTT 
GGTTTCTTAT TGGCTGGAAT TGGAGACCAA CTTGGAACCA TCTTTGGGAA AAGCATTGCA 
AGAGTGGAGA AGGTCTTTCG AAAAAAGCAA GTGAGTCAGA CCAAGATCCG GGTCATCTCA 
ACCATCCTGT TCATCTTGGC CGGCTGCATT GTGTTTGTGA CGATCCCTGC TGTCATCTTT 
AAGTACATCG AGGGCTGGAC GGCCTTGGAG TCCATTTACT TTGTGGTGGT CACTCTGACC 
ACGGTGGGCT TTGGTGATTT TGTGGCAGGG GGAAACGCTG GCATCAATTA TCGGGAGTGG 
TATAAGCCCC TAGTGTGGTT TTGGATCCTT GTTGGCCTTG CCTACTTTGC AGCTGTCCTC 
AGTATGATCG GAGATTGGCT ACGGGTTCTG TCCAAAAAGA CAAAAGAAGA GGTGGGTGAA 
ATCAAGGCCC ATGCGGCAGA GTGGAAGGCC AATGTCACGG CTGAGTTCCG GGAGACACGG 
CGAAGGCTCA GCGTGGAGAT CCACGATAAG CTGCAGCGGG CGGCCACCAT CCGCAGCATG 
GAGCGCCGGC GGCTGGGCCT GGACCAGCGG GCCCACTCAC TGGACATGCT GTCCCCCGAG 
AAGCGCTCTG TCTTTGCTGC CCTGGACACC GGCCGCTTCA AGGCCTCATC CCAGGAGAGC 
ATCAACAACC GGCCCAACAA CCTGCGCCTG AAGGGGCCGG AGCAGCTGAA CAAGCATGGG 
CAGGGTGCGT CCGAGGACAA CATCATCAAC AAGTTCGGGT CCACCTCCAG ACTCACCAAG 
AGGAAAAACA AGGACCTCAA AAAGACCTTG CCCGAGGACG TTCAGAAAAT CTACAAGACC 
TTCCGGAATT ACTCCCTGGA CGAGGAGAAG AAAGAGGAGG AGACGGAAAA GATGTGTAAC 
TCAGACAACT CCAGCACAGC CATGCTGACG GACTGTATCC AGCAGCACGC TGAGTTGGAG 
AACGGAATGA TACCCACGGA CACCAAAGAC CGGGAGCCGG AGAACAACTC ATTACTTGAA 
GACAGAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNK10 ( NM_021161.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_066984.1 Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant 1, mRNA.

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CloneID	OHu28007
Clone ID Related Accession (Same CDS sequence)	NM_021161.4
Accession Version	NM_021161.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1577376000000
Organism	Homo sapiens(human)
Product	potassium channel subfamily K member 10 isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL049834.3, AF279890.1, AL133279.7, AI693366.1 and U79244.1. On or before Sep 21, 2008 this sequence version replaced XM_001727026.1, XM_001723547.1, XM_001725959.1, NM_021161.3. Transcript Variant: This variant (1, also known as TREK2 splice variant a) encodes the shortest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.143135.1, SRR1803613.60524.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTTTTC TCTACACAGA CTTCTTTCTT TCCTTGGTGG CCGTTCCCGC AGCAGCACCG 
GTGTGCCAGC CCAAGAGCGC CACTAACGGG CAACCCCCGG CTCCGGCTCC GACTCCAACT 
CCGCGCCTGT CCATTTCCTC CCGAGCCACA GTGGTAGCCA GGATGGAAGG CACCTCCCAA 
GGGGGCTTGC AGACCGTCAT GAAGTGGAAG ACGGTGGTTG CCATCTTTGT GGTTGTGGTG 
GTCTACCTTG TCACTGGCGG TCTTGTCTTC CGGGCATTGG AGCAGCCCTT TGAGAGCAGC 
CAGAAGAATA CCATCGCCTT GGAGAAGGCG GAATTCCTGC GGGATCATGT CTGTGTGAGC 
CCCCAGGAGC TGGAGACGTT GATCCAGCAT GCTCTTGATG CTGACAATGC GGGAGTCAGT 
CCAATAGGAA ACTCTTCCAA CAACAGCAGC CACTGGGACC TCGGCAGTGC CTTTTTCTTT 
GCTGGAACTG TCATTACGAC CATAGGGTAT GGGAATATTG CTCCGAGCAC TGAAGGAGGC 
AAAATCTTTT GTATTTTATA TGCCATCTTT GGAATTCCAC TCTTTGGTTT CTTATTGGCT 
GGAATTGGAG ACCAACTTGG AACCATCTTT GGGAAAAGCA TTGCAAGAGT GGAGAAGGTC 
TTTCGAAAAA AGCAAGTGAG TCAGACCAAG ATCCGGGTCA TCTCAACCAT CCTGTTCATC 
TTGGCCGGCT GCATTGTGTT TGTGACGATC CCTGCTGTCA TCTTTAAGTA CATCGAGGGC 
TGGACGGCCT TGGAGTCCAT TTACTTTGTG GTGGTCACTC TGACCACGGT GGGCTTTGGT 
GATTTTGTGG CAGGGGGAAA CGCTGGCATC AATTATCGGG AGTGGTATAA GCCCCTAGTG 
TGGTTTTGGA TCCTTGTTGG CCTTGCCTAC TTTGCAGCTG TCCTCAGTAT GATCGGAGAT 
TGGCTACGGG TTCTGTCCAA AAAGACAAAA GAAGAGGTGG GTGAAATCAA GGCCCATGCG 
GCAGAGTGGA AGGCCAATGT CACGGCTGAG TTCCGGGAGA CACGGCGAAG GCTCAGCGTG 
GAGATCCACG ATAAGCTGCA GCGGGCGGCC ACCATCCGCA GCATGGAGCG CCGGCGGCTG 
GGCCTGGACC AGCGGGCCCA CTCACTGGAC ATGCTGTCCC CCGAGAAGCG CTCTGTCTTT 
GCTGCCCTGG ACACCGGCCG CTTCAAGGCC TCATCCCAGG AGAGCATCAA CAACCGGCCC 
AACAACCTGC GCCTGAAGGG GCCGGAGCAG CTGAACAAGC ATGGGCAGGG TGCGTCCGAG 
GACAACATCA TCAACAAGTT CGGGTCCACC TCCAGACTCA CCAAGAGGAA AAACAAGGAC 
CTCAAAAAGA CCTTGCCCGA GGACGTTCAG AAAATCTACA AGACCTTCCG GAATTACTCC 
CTGGACGAGG AGAAGAAAGA GGAGGAGACG GAAAAGATGT GTAACTCAGA CAACTCCAGC 
ACAGCCATGC TGACGGACTG TATCCAGCAG CACGCTGAGT TGGAGAACGG AATGATACCC 
ACGGACACCA AAGACCGGGA GCCGGAGAAC AACTCATTAC TTGAAGACAG AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066984.1
CDS	458..2074
Translation	MFFLYTDFFLSLVAVPAAAPVCQPKSATNGQPPAPAPTPTPRLSISSRATVVARMEGTSQGGLQTVMKWKTVVAIFVVVVVYLVTGGLVFRALEQPFESSQKNTIALEKAEFLRDHVCVSPQELETLIQHALDADNAGVSPIGNSSNNSSHWDLGSAFFFAGTVITTIGYGNIAPSTEGGKIFCILYAIFGIPLFGFLLAGIGDQLGTIFGKSIARVEKVFRKKQVSQTKIRVISTILFILAGCIVFVTIPAVIFKYIEGWTALESIYFVVVTLTTVGFGDFVAGGNAGINYREWYKPLVWFWILVGLAYFAAVLSMIGDWLRVLSKKTKEEVGEIKAHAAEWKANVTAEFRETRRRLSVEIHDKLQRAATIRSMERRRLGLDQRAHSLDMLSPEKRSVFAALDTGRFKASSQESINNRPNNLRLKGPEQLNKHGQGASEDNIINKFGSTSRLTKRKNKDLKKTLPEDVQKIYKTFRNYSLDEEKKEEETEKMCNSDNSSTAMLTDCIQQHAELENGMIPTDTKDREPENNSLLEDRN

Target ORF information:

RefSeq Version	NM_021161.4
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021161.4

ORF Insert Sequence:

ATGTTTTTTC TCTACACAGA CTTCTTTCTT TCCTTGGTGG CCGTTCCCGC AGCAGCACCG 
GTGTGCCAGC CCAAGAGCGC CACTAACGGG CAACCCCCGG CTCCGGCTCC GACTCCAACT 
CCGCGCCTGT CCATTTCCTC CCGAGCCACA GTGGTAGCCA GGATGGAAGG CACCTCCCAA 
GGGGGCTTGC AGACCGTCAT GAAGTGGAAG ACGGTGGTTG CCATCTTTGT GGTTGTGGTG 
GTCTACCTTG TCACTGGCGG TCTTGTCTTC CGGGCATTGG AGCAGCCCTT TGAGAGCAGC 
CAGAAGAATA CCATCGCCTT GGAGAAGGCG GAATTCCTGC GGGATCATGT CTGTGTGAGC 
CCCCAGGAGC TGGAGACGTT GATCCAGCAT GCTCTTGATG CTGACAATGC GGGAGTCAGT 
CCAATAGGAA ACTCTTCCAA CAACAGCAGC CACTGGGACC TCGGCAGTGC CTTTTTCTTT 
GCTGGAACTG TCATTACGAC CATAGGGTAT GGGAATATTG CTCCGAGCAC TGAAGGAGGC 
AAAATCTTTT GTATTTTATA TGCCATCTTT GGAATTCCAC TCTTTGGTTT CTTATTGGCT 
GGAATTGGAG ACCAACTTGG AACCATCTTT GGGAAAAGCA TTGCAAGAGT GGAGAAGGTC 
TTTCGAAAAA AGCAAGTGAG TCAGACCAAG ATCCGGGTCA TCTCAACCAT CCTGTTCATC 
TTGGCCGGCT GCATTGTGTT TGTGACGATC CCTGCTGTCA TCTTTAAGTA CATCGAGGGC 
TGGACGGCCT TGGAGTCCAT TTACTTTGTG GTGGTCACTC TGACCACGGT GGGCTTTGGT 
GATTTTGTGG CAGGGGGAAA CGCTGGCATC AATTATCGGG AGTGGTATAA GCCCCTAGTG 
TGGTTTTGGA TCCTTGTTGG CCTTGCCTAC TTTGCAGCTG TCCTCAGTAT GATCGGAGAT 
TGGCTACGGG TTCTGTCCAA AAAGACAAAA GAAGAGGTGG GTGAAATCAA GGCCCATGCG 
GCAGAGTGGA AGGCCAATGT CACGGCTGAG TTCCGGGAGA CACGGCGAAG GCTCAGCGTG 
GAGATCCACG ATAAGCTGCA GCGGGCGGCC ACCATCCGCA GCATGGAGCG CCGGCGGCTG 
GGCCTGGACC AGCGGGCCCA CTCACTGGAC ATGCTGTCCC CCGAGAAGCG CTCTGTCTTT 
GCTGCCCTGG ACACCGGCCG CTTCAAGGCC TCATCCCAGG AGAGCATCAA CAACCGGCCC 
AACAACCTGC GCCTGAAGGG GCCGGAGCAG CTGAACAAGC ATGGGCAGGG TGCGTCCGAG 
GACAACATCA TCAACAAGTT CGGGTCCACC TCCAGACTCA CCAAGAGGAA AAACAAGGAC 
CTCAAAAAGA CCTTGCCCGA GGACGTTCAG AAAATCTACA AGACCTTCCG GAATTACTCC 
CTGGACGAGG AGAAGAAAGA GGAGGAGACG GAAAAGATGT GTAACTCAGA CAACTCCAGC 
ACAGCCATGC TGACGGACTG TATCCAGCAG CACGCTGAGT TGGAGAACGG AATGATACCC 
ACGGACACCA AAGACCGGGA GCCGGAGAAC AACTCATTAC TTGAAGACAG AAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNK10 ( NM_138318.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_612191.1 Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28024
Clone ID Related Accession (Same CDS sequence)	NM_138318.2
Accession Version	NM_138318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1632bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1559750400000
Organism	Homo sapiens(human)
Product	potassium channel subfamily K member 10 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF385399.1, BC075021.2, EU978940.1, AF279890.1, AL133279.7, AI693366.1 and U79244.1. On Sep 20, 2008 this sequence version replaced NM_138318.1. Transcript Variant: This variant (3, also known as TREK2 splice variant b) has a different 5' terminal exon compared to transcript variant 1, and encodes a slightly longer isoform (3) with a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF385399.1, EU978940.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGATG GATTTAAGGG GGACAGGACT GAAGGCTGTC GCAGTGATTC AGTGGCCGTT 
CCCGCAGCAG CACCGGTGTG CCAGCCCAAG AGCGCCACTA ACGGGCAACC CCCGGCTCCG 
GCTCCGACTC CAACTCCGCG CCTGTCCATT TCCTCCCGAG CCACAGTGGT AGCCAGGATG 
GAAGGCACCT CCCAAGGGGG CTTGCAGACC GTCATGAAGT GGAAGACGGT GGTTGCCATC 
TTTGTGGTTG TGGTGGTCTA CCTTGTCACT GGCGGTCTTG TCTTCCGGGC ATTGGAGCAG 
CCCTTTGAGA GCAGCCAGAA GAATACCATC GCCTTGGAGA AGGCGGAATT CCTGCGGGAT 
CATGTCTGTG TGAGCCCCCA GGAGCTGGAG ACGTTGATCC AGCATGCTCT TGATGCTGAC 
AATGCGGGAG TCAGTCCAAT AGGAAACTCT TCCAACAACA GCAGCCACTG GGACCTCGGC 
AGTGCCTTTT TCTTTGCTGG AACTGTCATT ACGACCATAG GGTATGGGAA TATTGCTCCG 
AGCACTGAAG GAGGCAAAAT CTTTTGTATT TTATATGCCA TCTTTGGAAT TCCACTCTTT 
GGTTTCTTAT TGGCTGGAAT TGGAGACCAA CTTGGAACCA TCTTTGGGAA AAGCATTGCA 
AGAGTGGAGA AGGTCTTTCG AAAAAAGCAA GTGAGTCAGA CCAAGATCCG GGTCATCTCA 
ACCATCCTGT TCATCTTGGC CGGCTGCATT GTGTTTGTGA CGATCCCTGC TGTCATCTTT 
AAGTACATCG AGGGCTGGAC GGCCTTGGAG TCCATTTACT TTGTGGTGGT CACTCTGACC 
ACGGTGGGCT TTGGTGATTT TGTGGCAGGG GGAAACGCTG GCATCAATTA TCGGGAGTGG 
TATAAGCCCC TAGTGTGGTT TTGGATCCTT GTTGGCCTTG CCTACTTTGC AGCTGTCCTC 
AGTATGATCG GAGATTGGCT ACGGGTTCTG TCCAAAAAGA CAAAAGAAGA GGTGGGTGAA 
ATCAAGGCCC ATGCGGCAGA GTGGAAGGCC AATGTCACGG CTGAGTTCCG GGAGACACGG 
CGAAGGCTCA GCGTGGAGAT CCACGATAAG CTGCAGCGGG CGGCCACCAT CCGCAGCATG 
GAGCGCCGGC GGCTGGGCCT GGACCAGCGG GCCCACTCAC TGGACATGCT GTCCCCCGAG 
AAGCGCTCTG TCTTTGCTGC CCTGGACACC GGCCGCTTCA AGGCCTCATC CCAGGAGAGC 
ATCAACAACC GGCCCAACAA CCTGCGCCTG AAGGGGCCGG AGCAGCTGAA CAAGCATGGG 
CAGGGTGCGT CCGAGGACAA CATCATCAAC AAGTTCGGGT CCACCTCCAG ACTCACCAAG 
AGGAAAAACA AGGACCTCAA AAAGACCTTG CCCGAGGACG TTCAGAAAAT CTACAAGACC 
TTCCGGAATT ACTCCCTGGA CGAGGAGAAG AAAGAGGAGG AGACGGAAAA GATGTGTAAC 
TCAGACAACT CCAGCACAGC CATGCTGACG GACTGTATCC AGCAGCACGC TGAGTTGGAG 
AACGGAATGA TACCCACGGA CACCAAAGAC CGGGAGCCGG AGAACAACTC ATTACTTGAA 
GACAGAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612191.1
CDS	123..1754
Translation	MEDGFKGDRTEGCRSDSVAVPAAAPVCQPKSATNGQPPAPAPTPTPRLSISSRATVVARMEGTSQGGLQTVMKWKTVVAIFVVVVVYLVTGGLVFRALEQPFESSQKNTIALEKAEFLRDHVCVSPQELETLIQHALDADNAGVSPIGNSSNNSSHWDLGSAFFFAGTVITTIGYGNIAPSTEGGKIFCILYAIFGIPLFGFLLAGIGDQLGTIFGKSIARVEKVFRKKQVSQTKIRVISTILFILAGCIVFVTIPAVIFKYIEGWTALESIYFVVVTLTTVGFGDFVAGGNAGINYREWYKPLVWFWILVGLAYFAAVLSMIGDWLRVLSKKTKEEVGEIKAHAAEWKANVTAEFRETRRRLSVEIHDKLQRAATIRSMERRRLGLDQRAHSLDMLSPEKRSVFAALDTGRFKASSQESINNRPNNLRLKGPEQLNKHGQGASEDNIINKFGSTSRLTKRKNKDLKKTLPEDVQKIYKTFRNYSLDEEKKEEETEKMCNSDNSSTAMLTDCIQQHAELENGMIPTDTKDREPENNSLLEDRN

Target ORF information:

RefSeq Version	NM_138318.2
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium two pore domain channel subfamily K member 10 (KCNK10), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138318.2

ORF Insert Sequence:

ATGGAGGATG GATTTAAGGG GGACAGGACT GAAGGCTGTC GCAGTGATTC AGTGGCCGTT 
CCCGCAGCAG CACCGGTGTG CCAGCCCAAG AGCGCCACTA ACGGGCAACC CCCGGCTCCG 
GCTCCGACTC CAACTCCGCG CCTGTCCATT TCCTCCCGAG CCACAGTGGT AGCCAGGATG 
GAAGGCACCT CCCAAGGGGG CTTGCAGACC GTCATGAAGT GGAAGACGGT GGTTGCCATC 
TTTGTGGTTG TGGTGGTCTA CCTTGTCACT GGCGGTCTTG TCTTCCGGGC ATTGGAGCAG 
CCCTTTGAGA GCAGCCAGAA GAATACCATC GCCTTGGAGA AGGCGGAATT CCTGCGGGAT 
CATGTCTGTG TGAGCCCCCA GGAGCTGGAG ACGTTGATCC AGCATGCTCT TGATGCTGAC 
AATGCGGGAG TCAGTCCAAT AGGAAACTCT TCCAACAACA GCAGCCACTG GGACCTCGGC 
AGTGCCTTTT TCTTTGCTGG AACTGTCATT ACGACCATAG GGTATGGGAA TATTGCTCCG 
AGCACTGAAG GAGGCAAAAT CTTTTGTATT TTATATGCCA TCTTTGGAAT TCCACTCTTT 
GGTTTCTTAT TGGCTGGAAT TGGAGACCAA CTTGGAACCA TCTTTGGGAA AAGCATTGCA 
AGAGTGGAGA AGGTCTTTCG AAAAAAGCAA GTGAGTCAGA CCAAGATCCG GGTCATCTCA 
ACCATCCTGT TCATCTTGGC CGGCTGCATT GTGTTTGTGA CGATCCCTGC TGTCATCTTT 
AAGTACATCG AGGGCTGGAC GGCCTTGGAG TCCATTTACT TTGTGGTGGT CACTCTGACC 
ACGGTGGGCT TTGGTGATTT TGTGGCAGGG GGAAACGCTG GCATCAATTA TCGGGAGTGG 
TATAAGCCCC TAGTGTGGTT TTGGATCCTT GTTGGCCTTG CCTACTTTGC AGCTGTCCTC 
AGTATGATCG GAGATTGGCT ACGGGTTCTG TCCAAAAAGA CAAAAGAAGA GGTGGGTGAA 
ATCAAGGCCC ATGCGGCAGA GTGGAAGGCC AATGTCACGG CTGAGTTCCG GGAGACACGG 
CGAAGGCTCA GCGTGGAGAT CCACGATAAG CTGCAGCGGG CGGCCACCAT CCGCAGCATG 
GAGCGCCGGC GGCTGGGCCT GGACCAGCGG GCCCACTCAC TGGACATGCT GTCCCCCGAG 
AAGCGCTCTG TCTTTGCTGC CCTGGACACC GGCCGCTTCA AGGCCTCATC CCAGGAGAGC 
ATCAACAACC GGCCCAACAA CCTGCGCCTG AAGGGGCCGG AGCAGCTGAA CAAGCATGGG 
CAGGGTGCGT CCGAGGACAA CATCATCAAC AAGTTCGGGT CCACCTCCAG ACTCACCAAG 
AGGAAAAACA AGGACCTCAA AAAGACCTTG CCCGAGGACG TTCAGAAAAT CTACAAGACC 
TTCCGGAATT ACTCCCTGGA CGAGGAGAAG AAAGAGGAGG AGACGGAAAA GATGTGTAAC 
TCAGACAACT CCAGCACAGC CATGCTGACG GACTGTATCC AGCAGCACGC TGAGTTGGAG 
AACGGAATGA TACCCACGGA CACCAAAGAC CGGGAGCCGG AGAACAACTC ATTACTTGAA 
GACAGAAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The glycine hinge of transmembrane segment 2 modulates the subcellular localization and gating properties in TREK channels.
Biochemical and biophysical research communications490(3)1125-1131(2017 08)
Zhuo RG,Peng P,Zheng JQ,Zhang YL,Wen L,Wei XL,Ma XY

Polymodal activation of the TREK-2 K2P channel produces structurally distinct open states.
The Journal of general physiology147(6)497-505(2016 06)
McClenaghan C,Schewe M,Aryal P,Carpenter EP,Baukrowitz T,Tucker SJ

The isoforms generated by alternative translation initiation adopt similar conformation in the selectivity filter in TREK-2.
Journal of physiology and biochemistry71(4)601-10(2015 Dec)
Zhuo RG,Peng P,Liu XY,Zhang SZ,Xu JP,Zheng JQ,Wei XL,Ma XY

The association of DNA methylation and brain volume in healthy individuals and schizophrenia patients.
Schizophrenia research169(1-3)447-452(2015 Dec)
Liu J,Siyahhan Julnes P,Chen J,Ehrlich S,Walton E,Calhoun VD

How ion channels sense mechanical force: insights from mechanosensitive K2P channels TRAAK, TREK1, and TREK2.
Annals of the New York Academy of Sciences135220-32(2015 Sep)
Brohawn SG

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KCNK10 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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