HARS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HARS1
Entrez Gene ID	3035
Full Name	histidyl-tRNA synthetase
Synonyms	CMT2W,HRS,USH3B
General protein information	Preferred Names histidyl-tRNA synthetase Names histidine--tRNA ligase, cytoplasmic HisRS histidine translase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.3
Summary	Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012].
Disorder MIM:	142810
Disorder Html:	Usher syndrome type 3B, 614504 (3); Charcot-Marie-Tooth disease, axonal, type 2W, 616625 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001289092.1	NP_001276021.1	histidine--tRNA ligase, cytoplasmic isoform 5
NM_002109.6	NP_002100.2	histidine--tRNA ligase, cytoplasmic isoform 1
NM_001258042.2	NP_001244971.1	histidine--tRNA ligase, cytoplasmic isoform 4
NM_001289094.1	NP_001276023.1	histidine--tRNA ligase, cytoplasmic isoform 7
NM_002109.5	NP_002100.2	histidine--tRNA ligase, cytoplasmic isoform 1
NM_001289093.1	NP_001276022.1	histidine--tRNA ligase, cytoplasmic isoform 6
NM_001258040.2	NP_001244969.1	histidine--tRNA ligase, cytoplasmic isoform 2
NM_001258041.2	NP_001244970.1	histidine--tRNA ligase, cytoplasmic isoform 3

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0E06381g	XP_454235.1
Bos taurus (cattle)	HARS	NP_001039531.1
Magnaporthe oryzae (rice blast fungus)	MGG_15290	XP_003716150.1
Neurospora crassa	NCU06914	XP_959261.1
Caenorhabditis elegans (roundworm)	hars-1	NP_001023373.2
Schizosaccharomyces pombe (fission yeast)	hrs1	NP_596441.2
Canis lupus familiaris (dog)	HARS	XP_535213.2
Rattus norvegicus (Norway rat)	Hars	NP_001020585.1
Drosophila melanogaster (fruit fly)	Aats-his	NP_728180.1
Oryza sativa (rice)	Os05g0150900	NP_001054669.1
Homo sapiens (human)	HARS	NP_002100.2
Pan troglodytes (chimpanzee)	HARS	NP_001233308.1
Mus musculus (house mouse)	Hars	NP_032240.3
Gallus gallus (chicken)	HARS	NP_001006144.1
Danio rerio (zebrafish)	hars	NP_001004586.1
Saccharomyces cerevisiae (baker's yeast)	HTS1	NP_015358.1
Arabidopsis thaliana (thale cress)	AT3G02760	NP_186925.4
Eremothecium gossypii	AGOS_ACR187W	NP_983589.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000735	XP_311269.5
Xenopus tropicalis (tropical clawed frog)	LOC100489846	XP_002935955.2

Gene Ontology
Bibliography

Related articles in PubMed

Substrate interaction defects in histidyl-tRNA synthetase linked to dominant axonal peripheral neuropathy.
Abbott JA, Meyer-Schuman R, Lupo V, Feely S, Mademan I, Oprescu SN, Griffin LB, Alberti MA, Casasnovas C, Aharoni S, Basel-Vanagaite L, Züchner S, De Jonghe P, Baets J, Shy ME, Espinós C, Demeler B, Antonellis A, Francklyn C
Human mutation39(3)415-432(2018 03)

The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity.
Abbott JA, Guth E, Kim C, Regan C, Siu VM, Rupar CA, Demeler B, Francklyn CS, Robey-Bond SM
Biochemistry56(28)3619-3631(2017 07)

Evolutionary gain of highly divergent tRNA specificities by two isoforms of human histidyl-tRNA synthetase.
Lee YH, Chang CP, Cheng YJ, Kuo YY, Lin YS, Wang CC
Cellular and molecular life sciences : CMLS74(14)2663-2677(2017 07)

Loss of function mutations in HARS cause a spectrum of inherited peripheral neuropathies.
Safka Brozkova D, Deconinck T, Griffin LB, Ferbert A, Haberlova J, Mazanec R, Lassuthova P, Roth C, Pilunthanakul T, Rautenstrauss B, Janecke AR, Zavadakova P, Chrast R, Rivolta C, Zuchner S, Antonellis A, Beg AA, De Jonghe P, Senderek J, Seeman P, Baets J
Brain : a journal of neurology138(Pt 8)2161-72(2015 Aug)

Comparison of histidine recognition in human and trypanosomatid histidyl-tRNA synthetases.
Koh CY, Wetzel AB, de van der Schueren WJ, Hol WG
Biochimie106111-20(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following HARS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HARS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00954	NM_001258042.2 Latest version!	Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu01090	NM_001289094.1 Latest version!	Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu03181	NM_001258040.2 Latest version!	Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu03182	NM_001258041.2 Latest version!	Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu06577	NM_001289093.1 Latest version!	Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu27499	NM_001289092.1 Latest version!	Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu21691	NM_002109.6 Latest version!	Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
View Old Accession Versions >>
OHu21691	NM_002109.5	Homo sapiens histidyl-tRNA synthetase (HARS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5+	$265.30 $379.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HARS1 ( NM_001258042.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244971.1 Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 4, mRNA.

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CloneID	OHu00954
Clone ID Related Accession (Same CDS sequence)	NM_001258042.2
Accession Version	NM_001258042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Homo sapiens(human)
Product	histidine--tRNA ligase, cytoplasmic isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC394573.1, AK295219.1, AC116353.2 and AK225776.1. On Jan 15, 2014 this sequence version replaced NM_001258042.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon and uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295219.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCAGAGC GTGCGGCGCT GGAGGAGCTG GTGAAACTTC AGGGAGAGCG CGTGCGAGGC 
CTCAAGCAGC AGAAGGCCAG CGCCGAGCTG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGTCC TGATGAAAGC AAACAGAAAT TTGTGCTCAA AACCCCCAAG 
GAAACACTGA TGGGAAAGTA TGGGGAAGAC TCCAAGCTTA TCTATGACCT GAAGGACCAG 
GGCGGGGAGC TCCTGTCCCT TCGCTATGAC CTCACTGTTC CTTTTGCTCG GTATTTGGCA 
ATGAATAAAC TGACCAACAT TAAACGCTAC CACATAGCAA AGGATTTTGA CATTGCTGGG 
AACTTTGATC CCATGATCCC TGATGCAGAG TGCCTGAAGA TCATGTGCGA GATCCTGAGT 
TCACTTCAGA TAGGCGACTT CCTGGTCAAG GTAAACGATC GACGCATTCT AGATGGGATG 
TTTGCTATCT GTGGTGTTTC TGACAGCAAG TTCCGTACCA TCTGCTCCTC AGTAGACAAG 
CTGGACAAGG TGTCCTGGGA AGAGGTGAAG AATGAGATGG TGGGAGAGAA GGGCCTTGCA 
CCTGAGGTGG CTGACCGCAT TGGGGACTAT GTCCAGCAAC ATGGTGGGGT ATCCCTGGTG 
GAACAGCTGC TCCAGGATCC TAAACTATCC CAAAACAAGC AGGCCTTGGA GGGCCTGGGA 
GACCTGAAGT TGCTCTTTGA GTACCTGACC CTATTTGGCA TTGATGACAA AATCTCCTTT 
GACCTGAGCC TTGCTCGAGG GCTGGATTAC TACACTGGGG TGATCTATGA GGCAGTGCTG 
CTACAGACCC CAGCCCAGGC AGGGGAAGAG CCCCTGGGTG TGGGCAGTGT GGCTGCTGGA 
GGACGCTATG ATGGGCTAGT GGGCATGTTC GACCCCAAAG GGCGCAAGGT GCCATGTGTG 
GGGCTCAGCA TTGGGGTGGA GCGGATTTTC TCCATCGTGG AACAGAGACT AGAGGCTTTG 
GAGGAGAAGA TACGGACCAC GGAGACACAG GTGCTTGTGG CATCTGCACA GAAGAAGCTG 
CTAGAGGAAA GACTAAAGCT TGTCTCAGAA CTGTGGGATG CTGGGATCAA GGCTGAGCTG 
CTGTACAAGA AGAACCCAAA GCTACTGAAC CAGTTACAGT ACTGTGAGGA GGCAGGCATC 
CCACTGGTGG CTATCATCGG CGAGCAGGAA CTCAAGGATG GGGTCATCAA GCTCCGTTCA 
GTGACGAGCA GGGAAGAGGT GGATGTCCGA AGAGAAGACC TTGTGGAGGA AATCAAAAGG 
AGAACAGGCC AGCCCCTCTG CATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244971.1
CDS	424..1773
Translation	MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC

Target ORF information:

RefSeq Version	NM_001258042.2
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258042.2

ORF Insert Sequence:

ATGGCAGAGC GTGCGGCGCT GGAGGAGCTG GTGAAACTTC AGGGAGAGCG CGTGCGAGGC 
CTCAAGCAGC AGAAGGCCAG CGCCGAGCTG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGTCC TGATGAAAGC AAACAGAAAT TTGTGCTCAA AACCCCCAAG 
GAAACACTGA TGGGAAAGTA TGGGGAAGAC TCCAAGCTTA TCTATGACCT GAAGGACCAG 
GGCGGGGAGC TCCTGTCCCT TCGCTATGAC CTCACTGTTC CTTTTGCTCG GTATTTGGCA 
ATGAATAAAC TGACCAACAT TAAACGCTAC CACATAGCAA AGGATTTTGA CATTGCTGGG 
AACTTTGATC CCATGATCCC TGATGCAGAG TGCCTGAAGA TCATGTGCGA GATCCTGAGT 
TCACTTCAGA TAGGCGACTT CCTGGTCAAG GTAAACGATC GACGCATTCT AGATGGGATG 
TTTGCTATCT GTGGTGTTTC TGACAGCAAG TTCCGTACCA TCTGCTCCTC AGTAGACAAG 
CTGGACAAGG TGTCCTGGGA AGAGGTGAAG AATGAGATGG TGGGAGAGAA GGGCCTTGCA 
CCTGAGGTGG CTGACCGCAT TGGGGACTAT GTCCAGCAAC ATGGTGGGGT ATCCCTGGTG 
GAACAGCTGC TCCAGGATCC TAAACTATCC CAAAACAAGC AGGCCTTGGA GGGCCTGGGA 
GACCTGAAGT TGCTCTTTGA GTACCTGACC CTATTTGGCA TTGATGACAA AATCTCCTTT 
GACCTGAGCC TTGCTCGAGG GCTGGATTAC TACACTGGGG TGATCTATGA GGCAGTGCTG 
CTACAGACCC CAGCCCAGGC AGGGGAAGAG CCCCTGGGTG TGGGCAGTGT GGCTGCTGGA 
GGACGCTATG ATGGGCTAGT GGGCATGTTC GACCCCAAAG GGCGCAAGGT GCCATGTGTG 
GGGCTCAGCA TTGGGGTGGA GCGGATTTTC TCCATCGTGG AACAGAGACT AGAGGCTTTG 
GAGGAGAAGA TACGGACCAC GGAGACACAG GTGCTTGTGG CATCTGCACA GAAGAAGCTG 
CTAGAGGAAA GACTAAAGCT TGTCTCAGAA CTGTGGGATG CTGGGATCAA GGCTGAGCTG 
CTGTACAAGA AGAACCCAAA GCTACTGAAC CAGTTACAGT ACTGTGAGGA GGCAGGCATC 
CCACTGGTGG CTATCATCGG CGAGCAGGAA CTCAAGGATG GGGTCATCAA GCTCCGTTCA 
GTGACGAGCA GGGAAGAGGT GGATGTCCGA AGAGAAGACC TTGTGGAGGA AATCAAAAGG 
AGAACAGGCC AGCCCCTCTG CATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS1 ( NM_001289094.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276023.1 Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu01090
Clone ID Related Accession (Same CDS sequence)	NM_001289094.1
Accession Version	NM_001289094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Homo sapiens(human)
Product	histidine--tRNA ligase, cytoplasmic isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC116353.2 and AK307669.1. Transcript Variant: This variant (7) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded protein (isoform 7) lacks an internal region and is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK307669.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2159080, SAMEA2467147 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCGAGG AGGAGGTGGC GAAACTCCTG AAACTGAAGG CACAGCTGGG TCCTGATGAA 
AGCAAACAGA AATTTGTGCT CAAAACCCCC AAGGGCACAA GAGACTATAG TCCCCGGCAG 
ATGGCAGTTC GCGAGAAGGT GTTTGACGTA ATCATCCGTT GCTTCAAGCG CCACGGTGCA 
GAAGTCATTG ATACACCTGT ATTTGAACTA AAGGAAACAC TGATGGGAAA GTATGGGGAA 
GACTCCAAGC TTATCTATGA CCTGAAGGAC CAGGGCGGGG AGCTCCTGTC CCTTCGCTAT 
GACCTCACTG TTCCTTTTGC TCGGTATTTG GCAATGAATA AACTGACCAA CATTAAACGC 
TACCACATAG CAAAGGTATA TCGGCGGGAT AACCCAGCCA TGACCCGTGG CCGATACCGG 
GAATTCTACC AGTGTGATTT TGACATTGCT GGGAACTTTG ATCCCATGAT CCCTGATGCA 
GAGTGCCTGA AGATCATGTG CGAGATCCTG AGTTCACTTC AGATAGGCGA CTTCCTGGTC 
AAGGTAAACG ATCGACGCAT TCTAGATGGG ATGTTTGCTA TCTGTGGTGT TTCTGACAGC 
AAGTTCCGTA CCATCTGCTC CTCAGTAGAC AAGCTGGACA AGGTGTCCTG GGAAGAGGTG 
AAGAATGAGA TGGTGGGAGA GAAGGGCCTT GCACCTGAGG TGGCTGACCG CATTGGGGAC 
TATGTCCAGC AACATGGTGG GGTATCCCTG GTGGAACAGC TGCTCCAGGA TCCTAAACTA 
TCCCAAAACA AGCAGGCCTT GGAGGGCCTG GGAGACCTGA AGTTGCTCTT TGAGTACCTG 
ACCCTATTTG GCATTGATGA CAAAATCTCC TTTGACCTGA GCCTTGCTCG AGGGCTGGAT 
TACTACACTG GGGTGATCTA TGAGGCAGTG CTGCTACAGA CCCCAGCCCA GGCAGGGGAA 
GAGCCCCTGG GTGTGGGCAG TGTGGCTGCT GGAGGACGCT ATGATGGGCT AGTGGGCATG 
TTCGACCCCA AAGGGCGCAA GGTGCCATGT GTGGGGCTCA GCATTGGGGT GGAGCGGATT 
TTCTCCATCG TGGAACAGAG ACTAGAGGCT TTGGAGGAGA AGATACGGAC CACGGAGACA 
CAGGTGCTTG TGGCATCTGC ACAGAAGAAG CTGCTAGAGG AAAGACTAAA GCTTGTCTCA 
GAACTGTGGG ATGCTGGGAT CAAGGCTGAG CTGCTGTACA AGAAGAACCC AAAGCTACTG 
AACCAGTTAC AGTACTGTGA GGAGGCAGGC ATCCCACTGG TGGCTATCAT CGGCGAGCAG 
GAACTCAAGG ATGGGGTCAT CAAGCTCCGT TCAGTGACGA GCAGGGAAGA GGTGGATGTC 
CGAAGAGAAG ACCTTGTGGA GGAAATCAAA AGGAGAACAG GCCAGCCCCT CTGCATCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276023.1
CDS	424..1866
Translation	MIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC

Target ORF information:

RefSeq Version	NM_001289094.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289094.1

ORF Insert Sequence:

ATGATCGAGG AGGAGGTGGC GAAACTCCTG AAACTGAAGG CACAGCTGGG TCCTGATGAA 
AGCAAACAGA AATTTGTGCT CAAAACCCCC AAGGGCACAA GAGACTATAG TCCCCGGCAG 
ATGGCAGTTC GCGAGAAGGT GTTTGACGTA ATCATCCGTT GCTTCAAGCG CCACGGTGCA 
GAAGTCATTG ATACACCTGT ATTTGAACTA AAGGAAACAC TGATGGGAAA GTATGGGGAA 
GACTCCAAGC TTATCTATGA CCTGAAGGAC CAGGGCGGGG AGCTCCTGTC CCTTCGCTAT 
GACCTCACTG TTCCTTTTGC TCGGTATTTG GCAATGAATA AACTGACCAA CATTAAACGC 
TACCACATAG CAAAGGTATA TCGGCGGGAT AACCCAGCCA TGACCCGTGG CCGATACCGG 
GAATTCTACC AGTGTGATTT TGACATTGCT GGGAACTTTG ATCCCATGAT CCCTGATGCA 
GAGTGCCTGA AGATCATGTG CGAGATCCTG AGTTCACTTC AGATAGGCGA CTTCCTGGTC 
AAGGTAAACG ATCGACGCAT TCTAGATGGG ATGTTTGCTA TCTGTGGTGT TTCTGACAGC 
AAGTTCCGTA CCATCTGCTC CTCAGTAGAC AAGCTGGACA AGGTGTCCTG GGAAGAGGTG 
AAGAATGAGA TGGTGGGAGA GAAGGGCCTT GCACCTGAGG TGGCTGACCG CATTGGGGAC 
TATGTCCAGC AACATGGTGG GGTATCCCTG GTGGAACAGC TGCTCCAGGA TCCTAAACTA 
TCCCAAAACA AGCAGGCCTT GGAGGGCCTG GGAGACCTGA AGTTGCTCTT TGAGTACCTG 
ACCCTATTTG GCATTGATGA CAAAATCTCC TTTGACCTGA GCCTTGCTCG AGGGCTGGAT 
TACTACACTG GGGTGATCTA TGAGGCAGTG CTGCTACAGA CCCCAGCCCA GGCAGGGGAA 
GAGCCCCTGG GTGTGGGCAG TGTGGCTGCT GGAGGACGCT ATGATGGGCT AGTGGGCATG 
TTCGACCCCA AAGGGCGCAA GGTGCCATGT GTGGGGCTCA GCATTGGGGT GGAGCGGATT 
TTCTCCATCG TGGAACAGAG ACTAGAGGCT TTGGAGGAGA AGATACGGAC CACGGAGACA 
CAGGTGCTTG TGGCATCTGC ACAGAAGAAG CTGCTAGAGG AAAGACTAAA GCTTGTCTCA 
GAACTGTGGG ATGCTGGGAT CAAGGCTGAG CTGCTGTACA AGAAGAACCC AAAGCTACTG 
AACCAGTTAC AGTACTGTGA GGAGGCAGGC ATCCCACTGG TGGCTATCAT CGGCGAGCAG 
GAACTCAAGG ATGGGGTCAT CAAGCTCCGT TCAGTGACGA GCAGGGAAGA GGTGGATGTC 
CGAAGAGAAG ACCTTGTGGA GGAAATCAAA AGGAGAACAG GCCAGCCCCT CTGCATCTGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS1 ( NM_001258040.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244969.1 Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03181
Clone ID Related Accession (Same CDS sequence)	NM_001258040.2
Accession Version	NM_001258040.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Homo sapiens(human)
Product	histidine--tRNA ligase, cytoplasmic isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC394573.1, AK302295.1 and AK225776.1. On Jan 15, 2014 this sequence version replaced NM_001258040.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.229301.1, SRR1803616.263570.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCAGAGC GTGCGGCGCT GGAGGAGCTG GTGAAACTTC AGGGAGAGCG CGTGCGAGGC 
CTCAAGCAGC AGAAGGCCAG CGCCGAGCTG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGTCC TGATGAAAGC AAACAGAAAT TTGTGCTCAA AACCCCCAAG 
GAAACACTGA TGGGAAAGTA TGGGGAAGAC TCCAAGCTTA TCTATGACCT GAAGGACCAG 
GGCGGGGAGC TCCTGTCCCT TCGCTATGAC CTCACTGTTC CTTTTGCTCG GTATTTGGCA 
ATGAATAAAC TGACCAACAT TAAACGCTAC CACATAGCAA AGGTATATCG GCGGGATAAC 
CCAGCCATGA CCCGTGGCCG ATACCGGGAA TTCTACCAGT GTGATTTTGA CATTGCTGGG 
AACTTTGATC CCATGATCCC TGATGCAGAG TGCCTGAAGA TCATGTGCGA GATCCTGAGT 
TCACTTCAGA TAGGCGACTT CCTGGTCAAG GTAAACGATC GACGCATTCT AGATGGGATG 
TTTGCTATCT GTGGTGTTTC TGACAGCAAG TTCCGTACCA TCTGCTCCTC AGTAGACAAG 
CTGGACAAGG TGTCCTGGGA AGAGGTGAAG AATGAGATGG TGGGAGAGAA GGGCCTTGCA 
CCTGAGGTGG CTGACCGCAT TGGGGACTAT GTCCAGCAAC ATGGTGGGGT ATCCCTGGTG 
GAACAGCTGC TCCAGGATCC TAAACTATCC CAAAACAAGC AGGCCTTGGA GGGCCTGGGA 
GACCTGAAGT TGCTCTTTGA GTACCTGACC CTATTTGGCA TTGATGACAA AATCTCCTTT 
GACCTGAGCC TTGCTCGAGG GCTGGATTAC TACACTGGGG TGATCTATGA GGCAGTGCTG 
CTACAGACCC CAGCCCAGGC AGGGGAAGAG CCCCTGGGTG TGGGCAGTGT GGCTGCTGGA 
GGACGCTATG ATGGGCTAGT GGGCATGTTC GACCCCAAAG GGCGCAAGGT GCCATGTGTG 
GGGCTCAGCA TTGGGGTGGA GCGGATTTTC TCCATCGTGG AACAGAGACT AGAGGCTTTG 
GAGGAGAAGA TACGGACCAC GGAGACACAG GTGCTTGTGG CATCTGCACA GAAGAAGCTG 
CTAGAGGAAA GACTAAAGCT TGTCTCAGAA CTGTGGGATG CTGGGATCAA GGCTGAGCTG 
CTGTACAAGA AGAACCCAAA GCTACTGAAC CAGTTACAGT ACTGTGAGGA GGCAGGCATC 
CCACTGGTGG CTATCATCGG CGAGCAGGAA CTCAAGGATG GGGTCATCAA GCTCCGTTCA 
GTGACGAGCA GGGAAGAGGT GGATGTCCGA AGAGAAGACC TTGTGGAGGA AATCAAAAGG 
AGAACAGGCC AGCCCCTCTG CATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244969.1
CDS	424..1833
Translation	MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC

Target ORF information:

RefSeq Version	NM_001258040.2
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258040.2

ORF Insert Sequence:

ATGGCAGAGC GTGCGGCGCT GGAGGAGCTG GTGAAACTTC AGGGAGAGCG CGTGCGAGGC 
CTCAAGCAGC AGAAGGCCAG CGCCGAGCTG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGTCC TGATGAAAGC AAACAGAAAT TTGTGCTCAA AACCCCCAAG 
GAAACACTGA TGGGAAAGTA TGGGGAAGAC TCCAAGCTTA TCTATGACCT GAAGGACCAG 
GGCGGGGAGC TCCTGTCCCT TCGCTATGAC CTCACTGTTC CTTTTGCTCG GTATTTGGCA 
ATGAATAAAC TGACCAACAT TAAACGCTAC CACATAGCAA AGGTATATCG GCGGGATAAC 
CCAGCCATGA CCCGTGGCCG ATACCGGGAA TTCTACCAGT GTGATTTTGA CATTGCTGGG 
AACTTTGATC CCATGATCCC TGATGCAGAG TGCCTGAAGA TCATGTGCGA GATCCTGAGT 
TCACTTCAGA TAGGCGACTT CCTGGTCAAG GTAAACGATC GACGCATTCT AGATGGGATG 
TTTGCTATCT GTGGTGTTTC TGACAGCAAG TTCCGTACCA TCTGCTCCTC AGTAGACAAG 
CTGGACAAGG TGTCCTGGGA AGAGGTGAAG AATGAGATGG TGGGAGAGAA GGGCCTTGCA 
CCTGAGGTGG CTGACCGCAT TGGGGACTAT GTCCAGCAAC ATGGTGGGGT ATCCCTGGTG 
GAACAGCTGC TCCAGGATCC TAAACTATCC CAAAACAAGC AGGCCTTGGA GGGCCTGGGA 
GACCTGAAGT TGCTCTTTGA GTACCTGACC CTATTTGGCA TTGATGACAA AATCTCCTTT 
GACCTGAGCC TTGCTCGAGG GCTGGATTAC TACACTGGGG TGATCTATGA GGCAGTGCTG 
CTACAGACCC CAGCCCAGGC AGGGGAAGAG CCCCTGGGTG TGGGCAGTGT GGCTGCTGGA 
GGACGCTATG ATGGGCTAGT GGGCATGTTC GACCCCAAAG GGCGCAAGGT GCCATGTGTG 
GGGCTCAGCA TTGGGGTGGA GCGGATTTTC TCCATCGTGG AACAGAGACT AGAGGCTTTG 
GAGGAGAAGA TACGGACCAC GGAGACACAG GTGCTTGTGG CATCTGCACA GAAGAAGCTG 
CTAGAGGAAA GACTAAAGCT TGTCTCAGAA CTGTGGGATG CTGGGATCAA GGCTGAGCTG 
CTGTACAAGA AGAACCCAAA GCTACTGAAC CAGTTACAGT ACTGTGAGGA GGCAGGCATC 
CCACTGGTGG CTATCATCGG CGAGCAGGAA CTCAAGGATG GGGTCATCAA GCTCCGTTCA 
GTGACGAGCA GGGAAGAGGT GGATGTCCGA AGAGAAGACC TTGTGGAGGA AATCAAAAGG 
AGAACAGGCC AGCCCCTCTG CATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS1 ( NM_001258041.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001244970.1 Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 3, mRNA.

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CloneID	OHu03182
Clone ID Related Accession (Same CDS sequence)	NM_001258041.2
Accession Version	NM_001258041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Homo sapiens(human)
Product	histidine--tRNA ligase, cytoplasmic isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC394573.1 and AK225776.1. On Jan 15, 2014 this sequence version replaced NM_001258041.1. Transcript Variant: This variant (3) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225776.1, SRR1803615.218366.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCAGAGC GTGCGGCGCT GGAGGAGCTG GTGAAACTTC AGGGAGAGCG CGTGCGAGGC 
CTCAAGCAGC AGAAGGCCAG CGCCGAGCTG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGTCC TGATGAAAGC AAACAGAAAT TTGTGCTCAA AACCCCCAAG 
GGCACAAGAG ACTATAGTCC CCGGCAGATG GCAGTTCGCG AGAAGGTGTT TGACGTAATC 
ATCCGTTGCT TCAAGCGCCA CGGTGCAGAA GTCATTGATA CACCTGTATT TGAACTAAAG 
GAAACACTGA TGGGAAAGTA TGGGGAAGAC TCCAAGCTTA TCTATGACCT GAAGGACCAG 
GGCGGGGAGC TCCTGTCCCT TCGCTATGAC CTCACTGTTC CTTTTGCTCG GTATTTGGCA 
ATGAATAAAC TGACCAACAT TAAACGCTAC CACATAGCAA AGGATTTTGA CATTGCTGGG 
AACTTTGATC CCATGATCCC TGATGCAGAG TGCCTGAAGA TCATGTGCGA GATCCTGAGT 
TCACTTCAGA TAGGCGACTT CCTGGTCAAG GTAAACGATC GACGCATTCT AGATGGGATG 
TTTGCTATCT GTGGTGTTTC TGACAGCAAG TTCCGTACCA TCTGCTCCTC AGTAGACAAG 
CTGGACAAGG TGTCCTGGGA AGAGGTGAAG AATGAGATGG TGGGAGAGAA GGGCCTTGCA 
CCTGAGGTGG CTGACCGCAT TGGGGACTAT GTCCAGCAAC ATGGTGGGGT ATCCCTGGTG 
GAACAGCTGC TCCAGGATCC TAAACTATCC CAAAACAAGC AGGCCTTGGA GGGCCTGGGA 
GACCTGAAGT TGCTCTTTGA GTACCTGACC CTATTTGGCA TTGATGACAA AATCTCCTTT 
GACCTGAGCC TTGCTCGAGG GCTGGATTAC TACACTGGGG TGATCTATGA GGCAGTGCTG 
CTACAGACCC CAGCCCAGGC AGGGGAAGAG CCCCTGGGTG TGGGCAGTGT GGCTGCTGGA 
GGACGCTATG ATGGGCTAGT GGGCATGTTC GACCCCAAAG GGCGCAAGGT GCCATGTGTG 
GGGCTCAGCA TTGGGGTGGA GCGGATTTTC TCCATCGTGG AACAGAGACT AGAGGCTTTG 
GAGGAGAAGA TACGGACCAC GGAGACACAG GTGCTTGTGG CATCTGCACA GAAGAAGCTG 
CTAGAGGAAA GACTAAAGCT TGTCTCAGAA CTGTGGGATG CTGGGATCAA GGCTGAGCTG 
CTGTACAAGA AGAACCCAAA GCTACTGAAC CAGTTACAGT ACTGTGAGGA GGCAGGCATC 
CCACTGGTGG CTATCATCGG CGAGCAGGAA CTCAAGGATG GGGTCATCAA GCTCCGTTCA 
GTGACGAGCA GGGAAGAGGT GGATGTCCGA AGAGAAGACC TTGTGGAGGA AATCAAAAGG 
AGAACAGGCC AGCCCCTCTG CATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001244970.1
CDS	424..1893
Translation	MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC

Target ORF information:

RefSeq Version	NM_001258041.2
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258041.2

ORF Insert Sequence:

ATGGCAGAGC GTGCGGCGCT GGAGGAGCTG GTGAAACTTC AGGGAGAGCG CGTGCGAGGC 
CTCAAGCAGC AGAAGGCCAG CGCCGAGCTG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGTCC TGATGAAAGC AAACAGAAAT TTGTGCTCAA AACCCCCAAG 
GGCACAAGAG ACTATAGTCC CCGGCAGATG GCAGTTCGCG AGAAGGTGTT TGACGTAATC 
ATCCGTTGCT TCAAGCGCCA CGGTGCAGAA GTCATTGATA CACCTGTATT TGAACTAAAG 
GAAACACTGA TGGGAAAGTA TGGGGAAGAC TCCAAGCTTA TCTATGACCT GAAGGACCAG 
GGCGGGGAGC TCCTGTCCCT TCGCTATGAC CTCACTGTTC CTTTTGCTCG GTATTTGGCA 
ATGAATAAAC TGACCAACAT TAAACGCTAC CACATAGCAA AGGATTTTGA CATTGCTGGG 
AACTTTGATC CCATGATCCC TGATGCAGAG TGCCTGAAGA TCATGTGCGA GATCCTGAGT 
TCACTTCAGA TAGGCGACTT CCTGGTCAAG GTAAACGATC GACGCATTCT AGATGGGATG 
TTTGCTATCT GTGGTGTTTC TGACAGCAAG TTCCGTACCA TCTGCTCCTC AGTAGACAAG 
CTGGACAAGG TGTCCTGGGA AGAGGTGAAG AATGAGATGG TGGGAGAGAA GGGCCTTGCA 
CCTGAGGTGG CTGACCGCAT TGGGGACTAT GTCCAGCAAC ATGGTGGGGT ATCCCTGGTG 
GAACAGCTGC TCCAGGATCC TAAACTATCC CAAAACAAGC AGGCCTTGGA GGGCCTGGGA 
GACCTGAAGT TGCTCTTTGA GTACCTGACC CTATTTGGCA TTGATGACAA AATCTCCTTT 
GACCTGAGCC TTGCTCGAGG GCTGGATTAC TACACTGGGG TGATCTATGA GGCAGTGCTG 
CTACAGACCC CAGCCCAGGC AGGGGAAGAG CCCCTGGGTG TGGGCAGTGT GGCTGCTGGA 
GGACGCTATG ATGGGCTAGT GGGCATGTTC GACCCCAAAG GGCGCAAGGT GCCATGTGTG 
GGGCTCAGCA TTGGGGTGGA GCGGATTTTC TCCATCGTGG AACAGAGACT AGAGGCTTTG 
GAGGAGAAGA TACGGACCAC GGAGACACAG GTGCTTGTGG CATCTGCACA GAAGAAGCTG 
CTAGAGGAAA GACTAAAGCT TGTCTCAGAA CTGTGGGATG CTGGGATCAA GGCTGAGCTG 
CTGTACAAGA AGAACCCAAA GCTACTGAAC CAGTTACAGT ACTGTGAGGA GGCAGGCATC 
CCACTGGTGG CTATCATCGG CGAGCAGGAA CTCAAGGATG GGGTCATCAA GCTCCGTTCA 
GTGACGAGCA GGGAAGAGGT GGATGTCCGA AGAGAAGACC TTGTGGAGGA AATCAAAAGG 
AGAACAGGCC AGCCCCTCTG CATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS1 ( NM_001289093.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276022.1 Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 6, mRNA.

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CloneID	OHu06577
Clone ID Related Accession (Same CDS sequence)	NM_001289093.1
Accession Version	NM_001289093.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Homo sapiens(human)
Product	histidine--tRNA ligase, cytoplasmic isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC394573.1 and AK303778.1. On Jan 15, 2014 this sequence version replaced XM_005268430.1. Transcript Variant: This variant (6) lacks three alternate in-frame exons in the 5' coding region, compared to variant 1. The encoded protein (isoform 6) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303778.1, SRR1803613.142675.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAGC GTGCGGCGCT GGAGGAGCTG GTGAAACTTC AGGGAGAGCG CGTGCGAGGC 
CTCAAGCAGC AGAAGGCCAG CGCCGAGCTG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGTCC TGATGAAAGC AAACAGAAAT TTGTGCTCAA AACCCCCAAG 
GATTTTGACA TTGCTGGGAA CTTTGATCCC ATGATCCCTG ATGCAGAGTG CCTGAAGATC 
ATGTGCGAGA TCCTGAGTTC ACTTCAGATA GGCGACTTCC TGGTCAAGGT AAACGATCGA 
CGCATTCTAG ATGGGATGTT TGCTATCTGT GGTGTTTCTG ACAGCAAGTT CCGTACCATC 
TGCTCCTCAG TAGACAAGCT GGACAAGGTG TCCTGGGAAG AGGTGAAGAA TGAGATGGTG 
GGAGAGAAGG GCCTTGCACC TGAGGTGGCT GACCGCATTG GGGACTATGT CCAGCAACAT 
GGTGGGGTAT CCCTGGTGGA ACAGCTGCTC CAGGATCCTA AACTATCCCA AAACAAGCAG 
GCCTTGGAGG GCCTGGGAGA CCTGAAGTTG CTCTTTGAGT ACCTGACCCT ATTTGGCATT 
GATGACAAAA TCTCCTTTGA CCTGAGCCTT GCTCGAGGGC TGGATTACTA CACTGGGGTG 
ATCTATGAGG CAGTGCTGCT ACAGACCCCA GCCCAGGCAG GGGAAGAGCC CCTGGGTGTG 
GGCAGTGTGG CTGCTGGAGG ACGCTATGAT GGGCTAGTGG GCATGTTCGA CCCCAAAGGG 
CGCAAGGTGC CATGTGTGGG GCTCAGCATT GGGGTGGAGC GGATTTTCTC CATCGTGGAA 
CAGAGACTAG AGGCTTTGGA GGAGAAGATA CGGACCACGG AGACACAGGT GCTTGTGGCA 
TCTGCACAGA AGAAGCTGCT AGAGGAAAGA CTAAAGCTTG TCTCAGAACT GTGGGATGCT 
GGGATCAAGG CTGAGCTGCT GTACAAGAAG AACCCAAAGC TACTGAACCA GTTACAGTAC 
TGTGAGGAGG CAGGCATCCC ACTGGTGGCT ATCATCGGCG AGCAGGAACT CAAGGATGGG 
GTCATCAAGC TCCGTTCAGT GACGAGCAGG GAAGAGGTGG ATGTCCGAAG AGAAGACCTT 
GTGGAGGAAA TCAAAAGGAG AACAGGCCAG CCCCTCTGCA TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276022.1
CDS	424..1611
Translation	MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC

Target ORF information:

RefSeq Version	NM_001289093.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289093.1

ORF Insert Sequence:

ATGGCAGAGC GTGCGGCGCT GGAGGAGCTG GTGAAACTTC AGGGAGAGCG CGTGCGAGGC 
CTCAAGCAGC AGAAGGCCAG CGCCGAGCTG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGTCC TGATGAAAGC AAACAGAAAT TTGTGCTCAA AACCCCCAAG 
GATTTTGACA TTGCTGGGAA CTTTGATCCC ATGATCCCTG ATGCAGAGTG CCTGAAGATC 
ATGTGCGAGA TCCTGAGTTC ACTTCAGATA GGCGACTTCC TGGTCAAGGT AAACGATCGA 
CGCATTCTAG ATGGGATGTT TGCTATCTGT GGTGTTTCTG ACAGCAAGTT CCGTACCATC 
TGCTCCTCAG TAGACAAGCT GGACAAGGTG TCCTGGGAAG AGGTGAAGAA TGAGATGGTG 
GGAGAGAAGG GCCTTGCACC TGAGGTGGCT GACCGCATTG GGGACTATGT CCAGCAACAT 
GGTGGGGTAT CCCTGGTGGA ACAGCTGCTC CAGGATCCTA AACTATCCCA AAACAAGCAG 
GCCTTGGAGG GCCTGGGAGA CCTGAAGTTG CTCTTTGAGT ACCTGACCCT ATTTGGCATT 
GATGACAAAA TCTCCTTTGA CCTGAGCCTT GCTCGAGGGC TGGATTACTA CACTGGGGTG 
ATCTATGAGG CAGTGCTGCT ACAGACCCCA GCCCAGGCAG GGGAAGAGCC CCTGGGTGTG 
GGCAGTGTGG CTGCTGGAGG ACGCTATGAT GGGCTAGTGG GCATGTTCGA CCCCAAAGGG 
CGCAAGGTGC CATGTGTGGG GCTCAGCATT GGGGTGGAGC GGATTTTCTC CATCGTGGAA 
CAGAGACTAG AGGCTTTGGA GGAGAAGATA CGGACCACGG AGACACAGGT GCTTGTGGCA 
TCTGCACAGA AGAAGCTGCT AGAGGAAAGA CTAAAGCTTG TCTCAGAACT GTGGGATGCT 
GGGATCAAGG CTGAGCTGCT GTACAAGAAG AACCCAAAGC TACTGAACCA GTTACAGTAC 
TGTGAGGAGG CAGGCATCCC ACTGGTGGCT ATCATCGGCG AGCAGGAACT CAAGGATGGG 
GTCATCAAGC TCCGTTCAGT GACGAGCAGG GAAGAGGTGG ATGTCCGAAG AGAAGACCTT 
GTGGAGGAAA TCAAAAGGAG AACAGGCCAG CCCCTCTGCA TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS1 ( NM_001289092.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276021.1 Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu27499
Clone ID Related Accession (Same CDS sequence)	NM_001289092.1
Accession Version	NM_001289092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573747200000
Organism	Homo sapiens(human)
Product	histidine--tRNA ligase, cytoplasmic isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC394573.1, AK293154.1 and AK225776.1. On Jan 15, 2014 this sequence version replaced XM_005268429.1. Transcript Variant: This variant (5) lacks two alternate in-frame exons in the 5' coding region, compared to variant 1. The encoded protein (isoform 5) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.463905.1, AK293154.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAGC GTGCGGCGCT GGAGGAGCTG GTGAAACTTC AGGGAGAGCG CGTGCGAGGC 
CTCAAGCAGC AGAAGGCCAG CGCCGAGCTG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGTCC TGATGAAAGC AAACAGAAAT TTGTGCTCAA AACCCCCAAG 
GGCACAAGAG ACTATAGTCC CCGGCAGATG GCAGTTCGCG AGAAGGTGTT TGACGTAATC 
ATCCGTTGCT TCAAGCGCCA CGGTGCAGAA GTCATTGATA CACCTGTATT TGAACTAAAG 
GATTTTGACA TTGCTGGGAA CTTTGATCCC ATGATCCCTG ATGCAGAGTG CCTGAAGATC 
ATGTGCGAGA TCCTGAGTTC ACTTCAGATA GGCGACTTCC TGGTCAAGGT AAACGATCGA 
CGCATTCTAG ATGGGATGTT TGCTATCTGT GGTGTTTCTG ACAGCAAGTT CCGTACCATC 
TGCTCCTCAG TAGACAAGCT GGACAAGGTG TCCTGGGAAG AGGTGAAGAA TGAGATGGTG 
GGAGAGAAGG GCCTTGCACC TGAGGTGGCT GACCGCATTG GGGACTATGT CCAGCAACAT 
GGTGGGGTAT CCCTGGTGGA ACAGCTGCTC CAGGATCCTA AACTATCCCA AAACAAGCAG 
GCCTTGGAGG GCCTGGGAGA CCTGAAGTTG CTCTTTGAGT ACCTGACCCT ATTTGGCATT 
GATGACAAAA TCTCCTTTGA CCTGAGCCTT GCTCGAGGGC TGGATTACTA CACTGGGGTG 
ATCTATGAGG CAGTGCTGCT ACAGACCCCA GCCCAGGCAG GGGAAGAGCC CCTGGGTGTG 
GGCAGTGTGG CTGCTGGAGG ACGCTATGAT GGGCTAGTGG GCATGTTCGA CCCCAAAGGG 
CGCAAGGTGC CATGTGTGGG GCTCAGCATT GGGGTGGAGC GGATTTTCTC CATCGTGGAA 
CAGAGACTAG AGGCTTTGGA GGAGAAGATA CGGACCACGG AGACACAGGT GCTTGTGGCA 
TCTGCACAGA AGAAGCTGCT AGAGGAAAGA CTAAAGCTTG TCTCAGAACT GTGGGATGCT 
GGGATCAAGG CTGAGCTGCT GTACAAGAAG AACCCAAAGC TACTGAACCA GTTACAGTAC 
TGTGAGGAGG CAGGCATCCC ACTGGTGGCT ATCATCGGCG AGCAGGAACT CAAGGATGGG 
GTCATCAAGC TCCGTTCAGT GACGAGCAGG GAAGAGGTGG ATGTCCGAAG AGAAGACCTT 
GTGGAGGAAA TCAAAAGGAG AACAGGCCAG CCCCTCTGCA TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276021.1
CDS	424..1731
Translation	MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC

Target ORF information:

RefSeq Version	NM_001289092.1
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001289092.1

ORF Insert Sequence:

ATGGCAGAGC GTGCGGCGCT GGAGGAGCTG GTGAAACTTC AGGGAGAGCG CGTGCGAGGC 
CTCAAGCAGC AGAAGGCCAG CGCCGAGCTG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGTCC TGATGAAAGC AAACAGAAAT TTGTGCTCAA AACCCCCAAG 
GGCACAAGAG ACTATAGTCC CCGGCAGATG GCAGTTCGCG AGAAGGTGTT TGACGTAATC 
ATCCGTTGCT TCAAGCGCCA CGGTGCAGAA GTCATTGATA CACCTGTATT TGAACTAAAG 
GATTTTGACA TTGCTGGGAA CTTTGATCCC ATGATCCCTG ATGCAGAGTG CCTGAAGATC 
ATGTGCGAGA TCCTGAGTTC ACTTCAGATA GGCGACTTCC TGGTCAAGGT AAACGATCGA 
CGCATTCTAG ATGGGATGTT TGCTATCTGT GGTGTTTCTG ACAGCAAGTT CCGTACCATC 
TGCTCCTCAG TAGACAAGCT GGACAAGGTG TCCTGGGAAG AGGTGAAGAA TGAGATGGTG 
GGAGAGAAGG GCCTTGCACC TGAGGTGGCT GACCGCATTG GGGACTATGT CCAGCAACAT 
GGTGGGGTAT CCCTGGTGGA ACAGCTGCTC CAGGATCCTA AACTATCCCA AAACAAGCAG 
GCCTTGGAGG GCCTGGGAGA CCTGAAGTTG CTCTTTGAGT ACCTGACCCT ATTTGGCATT 
GATGACAAAA TCTCCTTTGA CCTGAGCCTT GCTCGAGGGC TGGATTACTA CACTGGGGTG 
ATCTATGAGG CAGTGCTGCT ACAGACCCCA GCCCAGGCAG GGGAAGAGCC CCTGGGTGTG 
GGCAGTGTGG CTGCTGGAGG ACGCTATGAT GGGCTAGTGG GCATGTTCGA CCCCAAAGGG 
CGCAAGGTGC CATGTGTGGG GCTCAGCATT GGGGTGGAGC GGATTTTCTC CATCGTGGAA 
CAGAGACTAG AGGCTTTGGA GGAGAAGATA CGGACCACGG AGACACAGGT GCTTGTGGCA 
TCTGCACAGA AGAAGCTGCT AGAGGAAAGA CTAAAGCTTG TCTCAGAACT GTGGGATGCT 
GGGATCAAGG CTGAGCTGCT GTACAAGAAG AACCCAAAGC TACTGAACCA GTTACAGTAC 
TGTGAGGAGG CAGGCATCCC ACTGGTGGCT ATCATCGGCG AGCAGGAACT CAAGGATGGG 
GTCATCAAGC TCCGTTCAGT GACGAGCAGG GAAGAGGTGG ATGTCCGAAG AGAAGACCTT 
GTGGAGGAAA TCAAAAGGAG AACAGGCCAG CCCCTCTGCA TCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS1 ( NM_002109.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_002100.2 Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 1, mRNA.

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CloneID	OHu21691
Clone ID Related Accession (Same CDS sequence)	NM_002109.6 , NM_002109.5
Accession Version	NM_002109.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1573833600000
Organism	Homo sapiens(human)
Product	histidine--tRNA ligase, cytoplasmic isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z11518.1 and AK225776.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002109.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.38063.1, SRR1660803.160355.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000504156.7/ ENSP00000425634.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGAGC GTGCGGCGCT GGAGGAGCTG GTGAAACTTC AGGGAGAGCG CGTGCGAGGC 
CTCAAGCAGC AGAAGGCCAG CGCCGAGCTG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGTCC TGATGAAAGC AAACAGAAAT TTGTGCTCAA AACCCCCAAG 
GGCACAAGAG ACTATAGTCC CCGGCAGATG GCAGTTCGCG AGAAGGTGTT TGACGTAATC 
ATCCGTTGCT TCAAGCGCCA CGGTGCAGAA GTCATTGATA CACCTGTATT TGAACTAAAG 
GAAACACTGA TGGGAAAGTA TGGGGAAGAC TCCAAGCTTA TCTATGACCT GAAGGACCAG 
GGCGGGGAGC TCCTGTCCCT TCGCTATGAC CTCACTGTTC CTTTTGCTCG GTATTTGGCA 
ATGAATAAAC TGACCAACAT TAAACGCTAC CACATAGCAA AGGTATATCG GCGGGATAAC 
CCAGCCATGA CCCGTGGCCG ATACCGGGAA TTCTACCAGT GTGATTTTGA CATTGCTGGG 
AACTTTGATC CCATGATCCC TGATGCAGAG TGCCTGAAGA TCATGTGCGA GATCCTGAGT 
TCACTTCAGA TAGGCGACTT CCTGGTCAAG GTAAACGATC GACGCATTCT AGATGGGATG 
TTTGCTATCT GTGGTGTTTC TGACAGCAAG TTCCGTACCA TCTGCTCCTC AGTAGACAAG 
CTGGACAAGG TGTCCTGGGA AGAGGTGAAG AATGAGATGG TGGGAGAGAA GGGCCTTGCA 
CCTGAGGTGG CTGACCGCAT TGGGGACTAT GTCCAGCAAC ATGGTGGGGT ATCCCTGGTG 
GAACAGCTGC TCCAGGATCC TAAACTATCC CAAAACAAGC AGGCCTTGGA GGGCCTGGGA 
GACCTGAAGT TGCTCTTTGA GTACCTGACC CTATTTGGCA TTGATGACAA AATCTCCTTT 
GACCTGAGCC TTGCTCGAGG GCTGGATTAC TACACTGGGG TGATCTATGA GGCAGTGCTG 
CTACAGACCC CAGCCCAGGC AGGGGAAGAG CCCCTGGGTG TGGGCAGTGT GGCTGCTGGA 
GGACGCTATG ATGGGCTAGT GGGCATGTTC GACCCCAAAG GGCGCAAGGT GCCATGTGTG 
GGGCTCAGCA TTGGGGTGGA GCGGATTTTC TCCATCGTGG AACAGAGACT AGAGGCTTTG 
GAGGAGAAGA TACGGACCAC GGAGACACAG GTGCTTGTGG CATCTGCACA GAAGAAGCTG 
CTAGAGGAAA GACTAAAGCT TGTCTCAGAA CTGTGGGATG CTGGGATCAA GGCTGAGCTG 
CTGTACAAGA AGAACCCAAA GCTACTGAAC CAGTTACAGT ACTGTGAGGA GGCAGGCATC 
CCACTGGTGG CTATCATCGG CGAGCAGGAA CTCAAGGATG GGGTCATCAA GCTCCGTTCA 
GTGACGAGCA GGGAAGAGGT GGATGTCCGA AGAGAAGACC TTGTGGAGGA AATCAAAAGG 
AGAACAGGCC AGCCCCTCTG CATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002100.2
CDS	67..1596
Translation	MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC

Target ORF information:

RefSeq Version	NM_002109.6
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase 1 (HARS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002109.6

ORF Insert Sequence:

ATGGCAGAGC GTGCGGCGCT GGAGGAGCTG GTGAAACTTC AGGGAGAGCG CGTGCGAGGC 
CTCAAGCAGC AGAAGGCCAG CGCCGAGCTG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGTCC TGATGAAAGC AAACAGAAAT TTGTGCTCAA AACCCCCAAG 
GGCACAAGAG ACTATAGTCC CCGGCAGATG GCAGTTCGCG AGAAGGTGTT TGACGTAATC 
ATCCGTTGCT TCAAGCGCCA CGGTGCAGAA GTCATTGATA CACCTGTATT TGAACTAAAG 
GAAACACTGA TGGGAAAGTA TGGGGAAGAC TCCAAGCTTA TCTATGACCT GAAGGACCAG 
GGCGGGGAGC TCCTGTCCCT TCGCTATGAC CTCACTGTTC CTTTTGCTCG GTATTTGGCA 
ATGAATAAAC TGACCAACAT TAAACGCTAC CACATAGCAA AGGTATATCG GCGGGATAAC 
CCAGCCATGA CCCGTGGCCG ATACCGGGAA TTCTACCAGT GTGATTTTGA CATTGCTGGG 
AACTTTGATC CCATGATCCC TGATGCAGAG TGCCTGAAGA TCATGTGCGA GATCCTGAGT 
TCACTTCAGA TAGGCGACTT CCTGGTCAAG GTAAACGATC GACGCATTCT AGATGGGATG 
TTTGCTATCT GTGGTGTTTC TGACAGCAAG TTCCGTACCA TCTGCTCCTC AGTAGACAAG 
CTGGACAAGG TGTCCTGGGA AGAGGTGAAG AATGAGATGG TGGGAGAGAA GGGCCTTGCA 
CCTGAGGTGG CTGACCGCAT TGGGGACTAT GTCCAGCAAC ATGGTGGGGT ATCCCTGGTG 
GAACAGCTGC TCCAGGATCC TAAACTATCC CAAAACAAGC AGGCCTTGGA GGGCCTGGGA 
GACCTGAAGT TGCTCTTTGA GTACCTGACC CTATTTGGCA TTGATGACAA AATCTCCTTT 
GACCTGAGCC TTGCTCGAGG GCTGGATTAC TACACTGGGG TGATCTATGA GGCAGTGCTG 
CTACAGACCC CAGCCCAGGC AGGGGAAGAG CCCCTGGGTG TGGGCAGTGT GGCTGCTGGA 
GGACGCTATG ATGGGCTAGT GGGCATGTTC GACCCCAAAG GGCGCAAGGT GCCATGTGTG 
GGGCTCAGCA TTGGGGTGGA GCGGATTTTC TCCATCGTGG AACAGAGACT AGAGGCTTTG 
GAGGAGAAGA TACGGACCAC GGAGACACAG GTGCTTGTGG CATCTGCACA GAAGAAGCTG 
CTAGAGGAAA GACTAAAGCT TGTCTCAGAA CTGTGGGATG CTGGGATCAA GGCTGAGCTG 
CTGTACAAGA AGAACCCAAA GCTACTGAAC CAGTTACAGT ACTGTGAGGA GGCAGGCATC 
CCACTGGTGG CTATCATCGG CGAGCAGGAA CTCAAGGATG GGGTCATCAA GCTCCGTTCA 
GTGACGAGCA GGGAAGAGGT GGATGTCCGA AGAGAAGACC TTGTGGAGGA AATCAAAAGG 
AGAACAGGCC AGCCCCTCTG CATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HARS1 ( NM_002109.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002100.2 Homo sapiens histidyl-tRNA synthetase (HARS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21691
Clone ID Related Accession (Same CDS sequence)	NM_002109.6 , NM_002109.5
Accession Version	NM_002109.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1530bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	1514304000000
Organism	Homo sapiens(human)
Product	histidine--tRNA ligase, cytoplasmic isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from Z11518.1 and AK225776.1. This sequence is a reference standard in the RefSeqGene project. On Jan 15, 2014 this sequence version replaced NM_002109.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.16843.1, SRR1163655.86606.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCAGAGC GTGCGGCGCT GGAGGAGCTG GTGAAACTTC AGGGAGAGCG CGTGCGAGGC 
CTCAAGCAGC AGAAGGCCAG CGCCGAGCTG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGTCC TGATGAAAGC AAACAGAAAT TTGTGCTCAA AACCCCCAAG 
GGCACAAGAG ACTATAGTCC CCGGCAGATG GCAGTTCGCG AGAAGGTGTT TGACGTAATC 
ATCCGTTGCT TCAAGCGCCA CGGTGCAGAA GTCATTGATA CACCTGTATT TGAACTAAAG 
GAAACACTGA TGGGAAAGTA TGGGGAAGAC TCCAAGCTTA TCTATGACCT GAAGGACCAG 
GGCGGGGAGC TCCTGTCCCT TCGCTATGAC CTCACTGTTC CTTTTGCTCG GTATTTGGCA 
ATGAATAAAC TGACCAACAT TAAACGCTAC CACATAGCAA AGGTATATCG GCGGGATAAC 
CCAGCCATGA CCCGTGGCCG ATACCGGGAA TTCTACCAGT GTGATTTTGA CATTGCTGGG 
AACTTTGATC CCATGATCCC TGATGCAGAG TGCCTGAAGA TCATGTGCGA GATCCTGAGT 
TCACTTCAGA TAGGCGACTT CCTGGTCAAG GTAAACGATC GACGCATTCT AGATGGGATG 
TTTGCTATCT GTGGTGTTTC TGACAGCAAG TTCCGTACCA TCTGCTCCTC AGTAGACAAG 
CTGGACAAGG TGTCCTGGGA AGAGGTGAAG AATGAGATGG TGGGAGAGAA GGGCCTTGCA 
CCTGAGGTGG CTGACCGCAT TGGGGACTAT GTCCAGCAAC ATGGTGGGGT ATCCCTGGTG 
GAACAGCTGC TCCAGGATCC TAAACTATCC CAAAACAAGC AGGCCTTGGA GGGCCTGGGA 
GACCTGAAGT TGCTCTTTGA GTACCTGACC CTATTTGGCA TTGATGACAA AATCTCCTTT 
GACCTGAGCC TTGCTCGAGG GCTGGATTAC TACACTGGGG TGATCTATGA GGCAGTGCTG 
CTACAGACCC CAGCCCAGGC AGGGGAAGAG CCCCTGGGTG TGGGCAGTGT GGCTGCTGGA 
GGACGCTATG ATGGGCTAGT GGGCATGTTC GACCCCAAAG GGCGCAAGGT GCCATGTGTG 
GGGCTCAGCA TTGGGGTGGA GCGGATTTTC TCCATCGTGG AACAGAGACT AGAGGCTTTG 
GAGGAGAAGA TACGGACCAC GGAGACACAG GTGCTTGTGG CATCTGCACA GAAGAAGCTG 
CTAGAGGAAA GACTAAAGCT TGTCTCAGAA CTGTGGGATG CTGGGATCAA GGCTGAGCTG 
CTGTACAAGA AGAACCCAAA GCTACTGAAC CAGTTACAGT ACTGTGAGGA GGCAGGCATC 
CCACTGGTGG CTATCATCGG CGAGCAGGAA CTCAAGGATG GGGTCATCAA GCTCCGTTCA 
GTGACGAGCA GGGAAGAGGT GGATGTCCGA AGAGAAGACC TTGTGGAGGA AATCAAAAGG 
AGAACAGGCC AGCCCCTCTG CATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002100.2
CDS	424..1953
Translation	MAERAALEELVKLQGERVRGLKQQKASAELIEEEVAKLLKLKAQLGPDESKQKFVLKTPKGTRDYSPRQMAVREKVFDVIIRCFKRHGAEVIDTPVFELKETLMGKYGEDSKLIYDLKDQGGELLSLRYDLTVPFARYLAMNKLTNIKRYHIAKVYRRDNPAMTRGRYREFYQCDFDIAGNFDPMIPDAECLKIMCEILSSLQIGDFLVKVNDRRILDGMFAICGVSDSKFRTICSSVDKLDKVSWEEVKNEMVGEKGLAPEVADRIGDYVQQHGGVSLVEQLLQDPKLSQNKQALEGLGDLKLLFEYLTLFGIDDKISFDLSLARGLDYYTGVIYEAVLLQTPAQAGEEPLGVGSVAAGGRYDGLVGMFDPKGRKVPCVGLSIGVERIFSIVEQRLEALEEKIRTTETQVLVASAQKKLLEERLKLVSELWDAGIKAELLYKKNPKLLNQLQYCEEAGIPLVAIIGEQELKDGVIKLRSVTSREEVDVRREDLVEEIKRRTGQPLCIC

Target ORF information:

RefSeq Version	NM_002109.5
Organism	Homo sapiens(human)
Definition	Homo sapiens histidyl-tRNA synthetase (HARS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002109.5

ORF Insert Sequence:

ATGGCAGAGC GTGCGGCGCT GGAGGAGCTG GTGAAACTTC AGGGAGAGCG CGTGCGAGGC 
CTCAAGCAGC AGAAGGCCAG CGCCGAGCTG ATCGAGGAGG AGGTGGCGAA ACTCCTGAAA 
CTGAAGGCAC AGCTGGGTCC TGATGAAAGC AAACAGAAAT TTGTGCTCAA AACCCCCAAG 
GGCACAAGAG ACTATAGTCC CCGGCAGATG GCAGTTCGCG AGAAGGTGTT TGACGTAATC 
ATCCGTTGCT TCAAGCGCCA CGGTGCAGAA GTCATTGATA CACCTGTATT TGAACTAAAG 
GAAACACTGA TGGGAAAGTA TGGGGAAGAC TCCAAGCTTA TCTATGACCT GAAGGACCAG 
GGCGGGGAGC TCCTGTCCCT TCGCTATGAC CTCACTGTTC CTTTTGCTCG GTATTTGGCA 
ATGAATAAAC TGACCAACAT TAAACGCTAC CACATAGCAA AGGTATATCG GCGGGATAAC 
CCAGCCATGA CCCGTGGCCG ATACCGGGAA TTCTACCAGT GTGATTTTGA CATTGCTGGG 
AACTTTGATC CCATGATCCC TGATGCAGAG TGCCTGAAGA TCATGTGCGA GATCCTGAGT 
TCACTTCAGA TAGGCGACTT CCTGGTCAAG GTAAACGATC GACGCATTCT AGATGGGATG 
TTTGCTATCT GTGGTGTTTC TGACAGCAAG TTCCGTACCA TCTGCTCCTC AGTAGACAAG 
CTGGACAAGG TGTCCTGGGA AGAGGTGAAG AATGAGATGG TGGGAGAGAA GGGCCTTGCA 
CCTGAGGTGG CTGACCGCAT TGGGGACTAT GTCCAGCAAC ATGGTGGGGT ATCCCTGGTG 
GAACAGCTGC TCCAGGATCC TAAACTATCC CAAAACAAGC AGGCCTTGGA GGGCCTGGGA 
GACCTGAAGT TGCTCTTTGA GTACCTGACC CTATTTGGCA TTGATGACAA AATCTCCTTT 
GACCTGAGCC TTGCTCGAGG GCTGGATTAC TACACTGGGG TGATCTATGA GGCAGTGCTG 
CTACAGACCC CAGCCCAGGC AGGGGAAGAG CCCCTGGGTG TGGGCAGTGT GGCTGCTGGA 
GGACGCTATG ATGGGCTAGT GGGCATGTTC GACCCCAAAG GGCGCAAGGT GCCATGTGTG 
GGGCTCAGCA TTGGGGTGGA GCGGATTTTC TCCATCGTGG AACAGAGACT AGAGGCTTTG 
GAGGAGAAGA TACGGACCAC GGAGACACAG GTGCTTGTGG CATCTGCACA GAAGAAGCTG 
CTAGAGGAAA GACTAAAGCT TGTCTCAGAA CTGTGGGATG CTGGGATCAA GGCTGAGCTG 
CTGTACAAGA AGAACCCAAA GCTACTGAAC CAGTTACAGT ACTGTGAGGA GGCAGGCATC 
CCACTGGTGG CTATCATCGG CGAGCAGGAA CTCAAGGATG GGGTCATCAA GCTCCGTTCA 
GTGACGAGCA GGGAAGAGGT GGATGTCCGA AGAGAAGACC TTGTGGAGGA AATCAAAAGG 
AGAACAGGCC AGCCCCTCTG CATCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Substrate interaction defects in histidyl-tRNA synthetase linked to dominant axonal peripheral neuropathy.
Human mutation39(3)415-432(2018 03)
Abbott JA,Meyer-Schuman R,Lupo V,Feely S,Mademan I,Oprescu SN,Griffin LB,Alberti MA,Casasnovas C,Aharoni S,Basel-Vanagaite L,Züchner S,De Jonghe P,Baets J,Shy ME,Espinós C,Demeler B,Antonellis A,Francklyn C

The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity.
Biochemistry56(28)3619-3631(2017 07)
Abbott JA,Guth E,Kim C,Regan C,Siu VM,Rupar CA,Demeler B,Francklyn CS,Robey-Bond SM

Evolutionary gain of highly divergent tRNA specificities by two isoforms of human histidyl-tRNA synthetase.
Cellular and molecular life sciences : CMLS74(14)2663-2677(2017 07)
Lee YH,Chang CP,Cheng YJ,Kuo YY,Lin YS,Wang CC

Loss of function mutations in HARS cause a spectrum of inherited peripheral neuropathies.
Brain : a journal of neurology138(Pt 8)2161-72(2015 Aug)
Safka Brozkova D,Deconinck T,Griffin LB,Ferbert A,Haberlova J,Mazanec R,Lassuthova P,Roth C,Pilunthanakul T,Rautenstrauss B,Janecke AR,Zavadakova P,Chrast R,Rivolta C,Zuchner S,Antonellis A,Beg AA,De Jonghe P,Senderek J,Seeman P,Baets J

Comparison of histidine recognition in human and trypanosomatid histidyl-tRNA synthetases.
Biochimie106111-20(2014 Nov)
Koh CY,Wetzel AB,de van der Schueren WJ,Hol WG

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HARS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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